Home

AquaMate Operator`s Manual

1. DPD H1480 FXD Chlorine Dioxide H1500 FXD H1510 EXD Direct Reading 0 1000 mg l 1520 H1520 FXD Chromium Hexavalent H1560 QNT H1570 QNT H1580 QNT Chromium Trivalent Direct Reading 0 20 0 g l 1550 H1550 FXD H1600 QNT COD Oxygen Demand H2700 QNT Chemical Reactor Digestion 0 150 mg l 2710 H2710 QNT Reactor Digestion Hg Free 0 150mgl H2715QNT Reactor Digestion 0 1500 mg l 2720 H2720 QNT Reactor Digestion Hg Free 0 1500mgil H2725 0NT Reactor Digestion 0 15 g l 2720 H2720 QNT 20 1000mg l 2730 H2730 QNT Colour True and Apparent Platinum Cobalt 0 500 units 1670 H1670 QNT Platinum Cobalt 0 500 units 1680 H1680 QNT Copper Porphyrin 0 210 0 ug 1720 H1720 QNT icinchoninate 0 5 000 mg 1700 H1700 QNT Bicinchoninate AccuVac 0 5 000 mg 1710 H1710 QNT Copper Autocatalytic 0 3 00 g l 1690 H1690 QNT Pyridine Pyrazalone 0 0 240 mg l 1750 H1750 QNT Crystal Violet 0 0 275 mg l 1850 H1850 QNT Fluoride PADNS 0 2 00 mg l H1900 QNT H1910 QNT Magnesium ecco ONT Hydrazine p Dimethylamino benzaldehyde 0 600 ug l 2050 H2050 QNT p Dimethylamino benzaldehyde 0 600 ug l 2060 H2060 QNT AccuVac D 2 fel D 5 o o o DPD 1 5 7 00 mgt 2100 H2100 FXD 2110 H2110 FXD i H2175 QNT H2160 QNT H2190 QNT H2195 QNT H2165 QNT H2170 QNT Iron Total 2175 2160 2190 2195 2165 erroVer AccuVac 2170
2. 54 English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS finale Method Range __ Program no Lead Fast Column Extraction 0 150 ug l 2210 H2210 QNT LeadTrak 0 300 ug l 2200 H2200 QNT Manganese PAN 0 0 700 mg l 2260 H2260 QNT Molybdenum Molybdate Nitrogen Ammonia Salicylate 0 0 80 mg l and free ammonia Nitrogen Nitrate Cadmium Reduction 0 5 0 mg l 2520 H2520 QNT Cadmium Reduction AccuVac 0 5 0 mg l 2525 H2525 QNT Cadmium Reduction 0 30 0 mg l 2530 H2530 QNT Cadmium Reduction AccuVac Chromotropic Acid TNT Nitrogen Nitrite Diazotization Diazotization AccuVac 0 0 3000 mg l Diazotization TNT errous Sulphate itanium Reduction TNT 4 Aminoantipyrine 0 0 200 mg l H2900 QNT Phosphonates Persulphate UV Oxidation 0 2 50 to 0 2950 H2950 QNT 125 mg l D n en Phosphorus Reactive AM Issue 3b 07 2001 English 55 AQUAMATE METHODS DISKS HACH Disk 2 Analyte Method Range Program no Hydrolyzable Direct Binary Complex 0 5 00 mg l H3200 QNT Compounds Silica Heteropoly Blue 0 1 600 mg l 3360 H3360 QNT Silicomolybdate 0 100 mg l 3350 H3350 QNT 0 0 700 mg l 3400 H3400 FXD SulfaVer 4 0 70 0 mg l 3450 H3450 QNT SulfaVer 4 AccuVac 0 70 0 mg l 3460 H3460 QNT Sulphide Methylene Blue 0 800
3. i 42 Operation aieiaiei aia iii 43 EEEIEI E E E E E TEE ETE 48 AM Issue 3b 07 2001 English Contents i Cell Programmer modes of operation 61 Removal and Refit nnns te ee 62 SuperSipper modes of operation 63 Sipper Calibration gaan inaia 64 MiniSipper modes of operation nr neneseerr rnnr 66 MiniSipper Calibration sse 67 CALIBRATION VALIDATION CAROUSEL CVC SIUE CHEM 69 CVC modes of operation rn rsresrrrnr nn 70 BOUM 71 Removal and Refit 72 Routine Maintenance i 75 Removal and Replacement of Lamps 75 Fault Finding Guide i 78 Connecting AquaMate to a PC sese 80 English AM Issue 3b 07 2001 INSTALLATION Getting Started with AquaMate spectrophotometers AquaMate spectrophotometers are entire instruments which are operated through their integral control panels Hard copy of results can be made with a pre installed internal printer or a separate external printer THIS SYSTEM IS DESIGNED TO BE USER INSTALLED So use the following procedures to quickly get your new spectrophotometer system in position and running the way you want it 1 Read the Safety instructions on page 3 of this manual
4. and bracketing Allowed operands include Measurements Constants entered via the numeric keypad Fixed amp Variable Factors input by the user at run time and uvcalc Results from preceeding equations Each formula may have up to 20 characters Equations will be saved with the current application method and uvcalc results saved with the current results Equations results units and pass fail results are included on the hardcopy output English AM Issue 3b 07 2001 UVCALC OPERATION a UVCALC appears as a menu item on both the FIXED and QUANT method pages Q Pressing enter when the UVCALC line is highlighted will present a list of 4 uvcalc equations Q Highlighting and entering one of these will present the EQUATION PARAMETERS page in the following format FORMULA TITLE UNITS TEST RESULTS NO UPPER LIMIT 0 000 LOWER LIMIT 0 000 In QUANT mode there is an additional parameter CONC VALUES YES O To create a formula select FORMULA and press ENTER A keyboard will appear with the following symbols M F R SPACE M Measurement On selecting this a pop up appears providing the choice between the different types of measurements available Up to 9 different measured results may be specified for each uvcalc equation in FIXED mode These measurements will comprise a combination of up to 9 different one off measurements measured at the start of the run only and one measurement wh
5. a A red warning stripe is printed on the paper within 1 metre of the end of the roll It is recommended that the roll is replaced as soon as the warning stripe is visible to avoid possible paper jams Q The printer is fitted with a Line Feed Button Press this once to switch the printer off line and feed the paper though the printer Press the button a second time to put the printer back on line Do not press the Line Feed Button during printing as this will cause a internal error condition which can only be rectified by restarting the instrument To replace the paper roll Q Cutthe paper at the end of the new roll in a V to make a point at the end of the paper Q Carefully feed the printer paper from the bottom of the roll i e shiny side down into the back of the printer using the Line Feed Button Q Keep the Line Feed Button depressed until the paper emerges squarely through the top slot of the printer housing AM Issue 3b 07 2001 English 69 MAINTENANCE MAINTENANCE Q Q 74 The information given in this section deals only with those parts of maintenance or service which can be safely carried out by the user Work other than that detailed should be carried out by a service engineer ALWAYS ENSURE THAT THE SAMPLE BEAM IS CLEAR BEFORE SWITCHING ON THE INSTRUMENT Failure to do so will produce abnormal results Low lamp energy values can be caused by leaving cells in the sample beam during energ
6. PRINT METHOD Prints the current method parameters and the standards table on the printer CALIBRATE Switches to the Standards Entry page QUANT STANDARDS Page Q This page lists the standards as defined in the QUANT METHOD Before the system can be calibrated each standard must have a concentration entered Q To enter Standard concentrations use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display the Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry Page with the highlight on the next standard on the list Up to 20 standards can be specified When all the standards have been entered press the ACCEPT function key to return to the QUANT Page with the new list of standards or CANCEL to return leaving the old list unchanged Q Ifa calibration has been done then the correlation coefficient and the equation are displayed a Ifa calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and Switches to the Quant Results screen Pressing ZERO BASE starts a zero using the current method 10 English AM Issue 3b 07 2001 QUANT STANDARDS VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration exists VIEW RESULTS Switch
7. MARK ABS A WAVELENGTH nm 1 0 472 447 0 2 2 002 463 0 Cc 0 375 480 0 Ello SCAN Q The list shows the positions and values of the peaks as calculated by the function selected in MANIPULATE PEAKS The measured values will be ABS T or INTENSITY depending on the current mode and are sorted by wavelength CHART OUTPUT Plots the spectrum on an analogue chart recorder PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page Q The page shows the positions and values of the wavelengths and the ratio as selected by the RATIO or CORR RATIO functions VIEW GRAPH Returns to the SCAN GRAPH page CHART OUTPUT Plots the spectrum on an analogue chart recorder PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page PEAK HEIGHT Page PEAK HEIGHT ABS A WAVELENGTH nm 0 472 447 0 2 002 463 0 0 375 480 0 AM Issue 3b 07 2001 Q This page shows the position and values of the wavelengths and the peak height as selected by the PEAK HEIGHT function VIEW GRAPH Returns to the SCAN GRAPH page CHART OUTPUT Plots the spectrum on an analogue chart recorder PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page English 23 UVCALC UVCALC Q Almost by definition quantitative analytical procedures are built around two fundamental key principles me
8. PRINT DIR Prints the list of files on the disk COPY ALL Copies all AquaMate method files from the Disk to the Library ENSURE THAT THE DISK CONTAINS ONLY VALID AQUAMATE DATA OR METHOD FILES 32 English AM Issue 3b 07 2001 SETUP SETUP Overview of Setup Options Q This section describes how to set up the instrument Q The main system setup options are available directly from the SETUP function key on the HOME page SETUP Page Q From the SETUP page move the cursor to the required option using the Up Down Arrow keys Select the option by pressing the ENTER key 1 DI NAMES 1 CLOCK Switches to the CLOCK page SETUP PRINTER Switches to the PRINTER page CLOCK ENVIRONMENT Switches to the ENVIRONMENT page PRINTER INITIALISE Switches to the OPTICAL INITIALISE WHITE LIGHT suede Wane LIGHT P I I Switches to the age G CVC Switches to the SETUP CVC Page RECORDER _________ 1 RECORDER Switches to the RECORDER Page To return to the HOME page press HOME CLOCK Page LS Re e CR i ER EC q CLOCK Q From this page the internal spectrophotometer clock calendar can be reset i TIME H Q To reset the time or date highlight the required parameter and et i Si press ENTER Enter the new value using the number keys and press ENTER Q Once all the parameters have been changed press ACCEPT I I I DERE The date or time will not
9. 50mm Rectangular mg l Ni 14785 14785P50 FXD 0 20 5 00 mg l 20mm Rectangular mg l Ni 14785 14785P20 FXD 0 10 5 00 mg l 10mm Rectangular mg l Ni 14785 14785P10 FXD 0 10 5 00 mg l 10mm Rectangular mg l Ni 14554 14554P10 FXD 5 00 mg l 10mm Rectangular mg l Fe 14761 ES P a O ER U Pre o x U 50 English AM Issue 3b 07 2001 Range Nitrogen Ammonia 0 01 2 00 mg l 0 01 2 60 mg l 0 01 2 00 mg l 0 01 2 60 mg l 0 20 8 00 mg l 0 30 10 00 mg l 0 20 8 00 mg l 0 30 10 00 mg l 0 5 16 0 mg l 0 6 21 0 mg l 0 5 16 0 mg l 0 6 21 0 mg l 4 0 80 0 mg l 5 0 100 0 mg l 4 0 80 0 mg l 5 0 100 0 mg l 0 010 0 500 mg l 0 010 0 650 mg l 0 03 1 50 mg l 0 04 1 90 mg l 0 05 3 00 mg l 0 06 3 90 mg l Nitrogen Nitrate 0 11 3 40 mg l 0 5 15 0 mg l 0 05 1 50 mg l 0 25 6 50 mg l 0 10 3 00 mg l 0 5 13 0 mg l 1 0 50 0 mg l 4 220 mg l 1 0 50 0 mg l 4 220 mg l 0 5 18 0 mg l 2 0 80 0 mg l 0 02 10 0 mg l 1 0 45 0 mg l 0 5 20 0 mg l 2 0 90 0 mg l 0 5 25 0 mgll 2 110 mg l 0 25 12 5 mg l 1 0 55 0 mg l 0 5 25 0 mgll 2 110 mg l 0 2 10 0 mg l 1 0 45 0 mg l 0 5 20 0 mg l 2 0 90 0 mg l AM Issue 3b 07 2001 AQUAMATE METHOD DISKS Cell Type Units Program 16mm Round mg l NH4 N 14739 mg l NH4 10mm Rect
10. AquaMate User Manual Note This manual is being revised so some of the information you will find in it is out of date Please accept our apologies for any confusion this may cause Any reference to Unicam Spectronic Instruments or Thermo Spectronic has changed to Thermo Electron Corporation and the contact and trademark information has also changed On the next page you will find a current disclaimer and up to date contact and trademark information Please contact Thermo Electron if you have any questions or concerns P N 949923046011 Thermo ELECTRON CORPORATION Copyright 2003 by Thermo Electron Corporation Madison WI 53711 The information in this publication is provided for reference only All information contained in this publication is believed to be correct and complete Thermo Electron Corporation shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material All product specifications as well as the information contained in this publication are subject to change without notice This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under our patent rights nor the rights of others We do not assume any liability arising out of any infringements of patents or other rights of third parties We make no warranty of any kind wi
11. From HOME page press SETUP select PRINTER using the cursor keys press ENTER The PRINTERS page is displayed with the default printer HP Mono Internal selected press ENTER again to display the Printer Menu and using the cursor keys select the required printer press ENTER THIS COMPLETES THE INSTALLATION OF AN AQUAMATE SYSTEM 2 English AM Issue 3b 07 2001 SAFETY SAFETY THIS SPECTROPHOTOMETER SYSTEM HAS BEEN DESIGNED TO BE USER INSTALLED READ THIS PAGE CAREFULLY BEFORE INSTALLING AND USING THE INSTRUMENT AND ITS ACCESSORIES The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 The instrument and accessories described in this manual are designed to be used by properly trained personnel only Adjustment maintenance and repair of exposed equipment must be carried out only by qualified personnel who are aware of the hazards involved Where indicated in the relevant manual certain maintenance processes may be carried out by the user who must be fully aware of and apply the following safety precautions For the correct and safe use of this instrument and its accessories it is essential that both operating and servicing personnel follow generally accepted safety procedures in addition to the safety precautions specified in this manual Specific warning and caution statements where applicable can be found throughout the manual Warning and c
12. How to set up a FIXED calculation M1 50 0 O On the FIXED page select the UVCALC item and press ENTER Q Select EQUATION 1 and press ENTER Q Select FORMULA and press ENTER Q Highlight M and press ENTER A pop up will appear giving the choice of ONCE ONLY CONSTANT MEASURE EACH RUN Q Highlight MEASURE EACH RUN and press ENTER Q Highlight and press ENTER Q Key in 50 a Press ACCEPT This will return you to the main uvcalc page Q Make appropriate entries for the TITLE and UNITS line Q Press ACCEPT twice This will return you to the FIXED page Q Insert sample and press RUN The results page will display the actual absorbance value and also the result of the calculation 26 English AM Issue 3b 07 2001 UVCALC How to add parameters to the end of an existing FIXED uvcalc equation e g to modify M1 50 0 by adding a weight correction so the equation becomes M1 50 0 F1 F2 Where F1 Nominal weight Fixed Factor F2 Actual weight Variable Factor 1 On the FIXED page go to the uvcalc line and press ENTER O Select EQUATION 1 and press ENTER The equation prepared earlier should be displayed O Select FORMULA and press ENTER The cursor should be at the end of the existing formula O Highlight and press ENTER A Highlight and press ENTER A Highlight F and press ENTER A pop up will appear giving the choice of FIXED FACTOR VARIABLE FACTOR O Highligh
13. OFF Sets Peak Table to Off No peaks information is produced as part of the scan PEAKS Picks the highest peaks in a spectrum up to a maximum of 10 peaks TRACK This function allows the Absorbance or other mode values to be reported at up to 10 user selected wavelengths To enter the Desired wavelengths select PEAK TABLE TRACK then press VIEW GRAPH You do not have to have a spectrum present on the graph to enter the selected wavelengths Press MANIPULATE and select TRACK For each wavelength move the cursor to the desired position press ENTER Once all the wavelengths have been entered go back to the SCAN METHOD page and save the method RATIO This function allows the ratio X A5to be automatically calculated at the end of the scan To enter the desired wavelengths select PEAK TABLE and press ENTER then select RATIO A pop up box Appears in which to enter the first wavelength Enter the desired wavelength and press ENTER Repeat for the second wavelength Once all the method parameters have been set save the method CORR RATIO This function allows the ratio of two wavelengths to be calculated relative to a third wavelength A A3 A A3 automatically at the end wavelength and press ENTER Repeat for the second and correction wavelengths Once all the method parameters have been set save the method PEAK HEIGHT This function allows the height of a peak to be calculated relative To a local baseline rather than y 0 To enter the
14. REPLICATES Enter number of replicates for each standard Q Sets the number of times each standard is measured maximum 3 Each value obtained is used in the calibration UNITS Enter units for concentration using the TEXT ENTRY page CURVE FIT Select from LINEAR LINEAR TO 0 QUADRATIC QUAD TO 0 Q Selects the curve fit algorithm used in the calibration LINEAR performs a linear calibration At least two standards are required LINEAR TOO performs a linear calibration forced through zero QUADRATIC performs a quadratic fit on the data At least three standards are required QUAD TO 0 performs a quadratic fit with the data forced through zero At least two standards are required Changing the curve fit will cause the existing calibration to be recalculated Any results associated with the previous calibration will be lost MEASURE STDS Toggles between YES and NO Q When YES and Standard concentrations have been entered from the Quant Standards page pressing Calibrate causes the instrument to prompt the user to put the standard in the beam and press Run to measure for each Standard in turn Q When NO pressing Calibrate causes the system to prompt for an absorbance to be entered manually for each Standard effectively enabling a calibration originating elsewhere to be entered TIMER S Displays the AquaMateTimers Page Up to four countdown timers may be defined Use the arrow keys to move around the list of timer
15. indicating the the presence of the accessory and its setting If this status box is displayed then the Cell Programmer can be moved manually using the lt gt arrow keys To step foward backward many positions repeated pressing of the key will be remembered by the system p Q0 This page allows the Cell Programmer to be set up for the required analysis To reach this page highlight the CELL PROGRAMMER option from the I I CELL POS D QUANT or FIXED method pages and press MODE MANUAL ENTER 1 REF MODE OFF I i LAST CELL E CELL CYCLES 5 A Alternatively press ACCESSORIES from the SPEED HIGH HOME page then select CELL PROG and press Emu a I ENTER CELL POS Used to change the current cell position using arrow keys This change is reflected in the Status box MODE Selects from MANUAL RUN amp STEP AUTO OFF MANUAL Cell position is only changed by use of the key on the keyboard RUN amp STEP Allows measurement on the current cell then automatically moves to the next cell ready for the next measurement AUTO Performs a measurement on each of the cells in turn OFF Turns off the Cell Programmer The instrument now behaves as if it has a single cell only The number of cells automatically measured in sequence maximum of 7 will be dependent on the value of the LAST CELL parameter REF MODE Toggles between ON and OFF with Enter Q Wh
16. 10mm Rectangular mg l PO4 P 14848P10 FXD 0 2 15 0 mg l mg l PO4 0 05 5 00 mg l 16mm Round mg l PO4 P 14543 14543P16 FXD 0 2 15 3 mg l mg l PO4 0 03 2 50 mg l 20mm Rectangular mg l PO4 P_ 14543 14543P20 FXD 0 10 7 50 mg l mg l PO4 0 05 5 00 mg l 10mm Rectangular mg l PO4 P_ 14543 14543P10 FXD 0 2 15 0 mg l mg l PO4 0 5 25 mg l 16mm Round mglPO P 14729 14729P16 FXD 1 5 75 0 mg l mg l PO4 P 3 0 5 25 mg l 10mm Rectangular mg l PO4 14729 14729P10 FXD 1 5 75 0 mg l mg l PO4 Phosphorus VM 0 5 25 0 mg l 16mm Round mg l PO4 P 14546 14546P16 FXD 1 5 75 0 mg l mg l PO4 0 5 15 0 mg l 20mm Rectangular mg l PO4 P 14546 14546P20 FXD 1 5 45 0 mg l mg l PO 1 0 30 0 mg l 10mm Rectangular mg l PO4 P 14546 14546P10 FXD 3 0 90 0 mg l mg l PO 45 3 0 5 15 0 mg l 20mm Rectangular mg l PO4 P 14842 14842P20 FXD 1 5 45 0 mg l mg l PO 1 0 30 0 mg l 10mm Rectangular mg l PO4 P 14842 14842P10 FXD 3 0 90 0 mg l mg l PO4 5 0 50 0 mg l 20mm Rectangular mg l K 14562 14562P20 FXD 5 0 50 0 mg l 10mm Rectangular mg l K 14562 14562P10 FXD 0 005 0 750 mg l 50mm Rectangular mgll Si 14794 14794P50 FXD 5 0 50 0 mg l 16mm Round mg l K 14562 14562P16 FXD 0 05 2 50 mg l 20mm Rectangular mg l Si 14794 14794P20 FXD 0 1 5 00 mg l 10mm Rectangular mg l Si 14794 14794P10 FXD 52 English AM Issue 3b 07 2001 AQUAMATE METHO
17. HISTORY FILE name and the instrument history is saved in CSV format which may be read by a suitable spreadsheet or text SAVE HISTORY ON DISK editor CLEAR HISTORY PRINT HISTORY CLEAR HISTORY Clears the Instrument History PRINT HISTORY The Instrument History is printed out Make sure the printer is connected and ready before selecting PRINT HISTORY The History File contains a maximum of 400 entries When the number of entries reaches 390 a warning message is displayed and it is necessary for the Administrator or a user with the History File privilege save to disk and or print the existing history file then clear the history file to make room for more entries 0000 HISTORY FILE Appears when History File is enabled CHANGE USERS Available only to Administrator when User Log on enabled SETUP PAGE Returns to the SETUP page AM Issue 3b 07 2001 English 37 SETUP OPTICAL INITIALISATION Page This page is used to reset the instrument and define its initialisation and default baseline These procedures ensure the optimum performance of the spectrophotometer INITIALISATION TYPE When selected this displays a pop up menu to choose between initialisation of optics or baseline OPTICS During initialisation the instrument performs some simple hardware checks calculates various data tables and measures the dark current The filter wheel is then initialised before the instrument drives to the default wavel
18. Switches to the DISK page COPY ALL Copies all files from Library to Disk FORMAT LIBRARY Clears all files from the library THIS WILL DELETE ALL THE FILES IN THE LIBRARY PRINT DIR Prints a list of all the files in the Library Using the Library LIBRARY TYPE ID FILE NAME M QUANT UV123 AB123B QNT M FIXED DATAI FXD FXD D QUANT QNT D FIXED FXD LOAD M FIXED RENAME FXD SAVE TO DISK DELETE 76 LIBRARY SPACE REMAINING HIGHLIGHT FILE AND PRESS ENTER PRINT FORMAT COPY VIEW DIR LIBRARY ALL DISK To perform an operation on a library file first select the file To do this move the cursor to highlight the required file using the Up Down Arrow keys If the file does not appear on the list the gt and lt arrow keys will scroll one page at a time down and up the list There is a slight delay after the key is pressed while the next section of the directory is loaded Once the required file is in the window move the cursor to it using the Up Down arrow keys as above With the required file highlighted press ENTER to display the LIBRARY pop up menu Use the Up Down arrow keys to highlight the required operation and press ENTER LOAD Loads the file from the Library RENAME Switches to the SAVE RENAME page where file name and description can be changed SAVE TO DISK Copies the highlighted file to the disk DELETE Removes the highlighted file from the library AM Issue 3b 07 2001 English
19. 14561P10 EXD 14800P50 FXD 0 005 0 250 mg l 20mm Rectangular mg l CN 14800 14800P20 FXD 0 010 0 500 mg l 10mm Rectangular mg l CN 14800 14800P10 FXD 0 002 0 100 mg l 50mm Rectangular mg l CN 109701 09701P50 FXD 0 005 0 250 mg l 20mm Rectangular mg l CN 109701 09701P20 FXD 0 010 0 500 mg l 10mm Rectangular mg l CN 109701 09701P10 FXD AM Issue 3b 07 2001 English AQUAMATE METHODS DISKS EDITI Cell Type Units Program AquaMate File no Fluoride 0 10 1 50 mg l 16mm Round mg l F 14557 14557P16 FXD 0 10 1 50 mg l 20mm Rectangular mg l F 14557 14556P20 FXD 0 10 1 50 mg l 10mm Rectangular mg l F 14557 14557P10 FXD 0 025 0 500 mg l 50mm Rectangular mg l F 14557 14557P50 FXD 14500P16 FXD 0 05 6 00 mg l 20mm Rectangular 14500P20 FXD 14500P10 FXD 14678P50 FXD 14678P20 FXD 14678P10 FXD 14821P10 FXD Hardness Residual 14683P16 FXD 14683P20 FXD 0 50 5 00 mg l 10mm Rectangular mg l Ca 14683 14683P10 FXD Hardness Total 5 150 mg l 16mm Round mg l Ca 14565 14565P16 FXD 5 100 mg l 20mm Rectangular mg l Ca 14565 14565P20 FXD 150 mg l 10mm Rectangular mg l Ca 14565 14565P10 FXD Hydrazine 0 02 1 00 mg l 50mm Rectangular mg l N2H4 14797 14797P50 FXD 0 10 2 50 mg l 20mm Rectangular mg l N2H4 14797 14797P20 FXD 0 20 5 00 mg l 10mm R
20. 2 Check the component parts of the system against the Despatch Note and Packing List Immediately report by telephone confirmed in writing any discrepancies 3 Find a suitable location see below AVOID STATIC ELECTRICITY 4 Connect the supplied power cord to the spectrophotometer Connect power cord here tn 5 Ensure NO SAMPLE S are in place in the sample compartment Turn the spectrophotometer on AM Issue 3b 07 2001 English 1 INSTALLATION 6 Local Control display shows this sequence if OK V appear sequentially as each test is completed SPECTROPHOTOMETER INITIALISING 4 INITIALISE OPTICS 4 TEST W LAMP 4 INITIALISE MONOCHROMATOR 4 TEST OPTICS 4 OPTIMISE MONOCHROMATOR 4 SET DEFAULTS Approximately PLEASE WAIT 3 minutes duis THIS COMPLETES THE x INSTALLATION OF AN DSK AQUAMATE SYSTEM IF YOU WISH TO INSTRUMENT HOURS 1245 INSTALL AN EXTERNAL PRINTER SETUP CAL ACCESS LAMPS REMOTE GO TO POINT T VAL ORIES Connecting up a Printer to an AquaMate system PRINTERS 7 ENSURE THAT THE RM ee MONO SPECTROPHOTOMETER IS TURNED OFF EPSON 9 PIN HP LASERJET a Now connect the printer to the HP 690C Parallel port on the HP 400 INTERNAL spectrophotometer using the cable supplied SETUP PAGE 8 To set up the Printer in the software POWER UP THE SYSTEM AND ALLOW TO INITIALISE
21. 31 LIBRARY DISK Page This page allows access to the instrument disk drive It lists all the files on the disk currently in the instrument drive including file type description and file name FILE TYPE This describes the type of information contained in the file M QUANT Quant method including calibration D QUANT Quant results including method and calibration M FIXED Fixed wavelength method D FIXED Fixed wavelength results and method M SCAN Scan method Q Move the cursor to highlight the required file using the Up Down Arrow keys If the file does not appear on the list the gt and lt arrow keys will scroll one page at a time down and up the list There is a slight delay after the key is pressed while the next section of the directory is loaded Once the required file is in the window move the cursor to it using the Up Down arrow keys as above Q When the required file is highlighted select the file by pressing the ENTER key The popup menu will appear Then highlight the required operation and press ENTER LOAD Loads the file from Disk RENAME Switches to the SAVE RENAME page were file name and description can be changed DELETE Removes the highlighted file from the Disk DISK TYPE ID FILENAME VIEW LIBRARY Switches to the LIBRARY page READ DISK Reads the disk and refreshes the directory listing FORMAT DISK Formats the disk as a 1 44 Mb disk THIS WILL DELETE ALL THE FILES ON THE DISK
22. CHANGE MODE function key to set the OPERATING MODE as MULTIPLE USE or SINGLE USE In SINGLE USE mode the timers will be run before the first measurement only When the operating mode is set to MULTIPLE USE all timers will be used before each measurement Use the RUN TIMERS function key to operate the timers without initiating a measurement sequence Once the timers have been set up press the ACCEPT function key to accept the new list or the CANCEL function key to return to the FIXED METHOD page without changing the timers 14 English AM Issue 3b 07 2001 FIXED a When RUN is pressed to start a measurement the specified sequence of timers will be started with the remaining time for the current timer being displayed on the screen Any timer may be interrupted by selecting the STOP softkey Once the last timer has finished the measurement will be made in the normal way N B Timers can be used in conjunction with a sipper in AUTOMATIC mode They cannot be used with a cell programmer in AUTO mode LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Not available in AquaMate Vis Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 or W overrides any changeover and the selected lamp will be used regardless of the wavelength set OPERATOR Switches to the TEXT ENTRY screen Q The operator name is automatically saved with the method and any data produced by the met
23. FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater The function key label shows the next speed ie the opposite to the one selected CLEAR ALL Clears all the markers and the TRACK TABLE PRINT GRAPH Provides a hardcopy of the results showing the markers and x and y axis values SCAN GRAPH Returns to the SCAN GRAPH page RESCALE Q This option gives pop up menus for changing the graph x and y axis scales AUTO GRAPH HIGH Q Move the cursor to one of the options and press ENTER GRAPH LOW t lect ii GRAPH START O Select an operation GRAPH STOP PROCEED AUTO Displays the SCAN GRAPH with the x and y axes rescaled so that the Spectrum fills the screen GRAPH HIGH Pops up a window for entry of the GRAPH HIGH limit GRAPH LOW Pops up a window for entry of the GRAPH LOW limit GRAPH START Pops up a window for entry of the required start wavelength GRAPH STOP Pops up a window for entry of the required stop wavelength PROCEED Used after GRAPH HIGH GRAPH LOW GRAPH START or GRAPH STOP to return to the SCAN GRAPH page with the graph rescaled using the new parameters AM Issue 3b 07 2001 English 21 SCAN MODE Select from ABS T ABS Selects Absorbance T Selects transmittance PEAKS Q This option enables the spectrum to be automatically searched for
24. FXD i 50mm Rectangular LCW 028 W028P50 FXD 0 005 0 40 mg l i 0 8 20 mg l i 11mm round WO28CT FXD 0 4 10 mg l i 20 100 mg l i 10 50 mg l Surfactants Determination 0 2 2 0 mg l LCK 332 K332CT FXD 50mm Rectangular LCW 018 W018P50 FXD LCK360 K360CT FXD Sulphite 0 1 5 0 mg l 10mm Rectangular LCW 054 W054P10 FXD 0 5 25 0 mg l 0 02 0 80 mg l 58 English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS CHEMetrics Disk 4 Method Range Ammonia Nessler 0 7mg L Nessler 0 14mg L 0 9 mg L Chlorine DDPD 2513 C2513 FXD 0 Amg L 0 6mg L COLTE po p Wmec emspo Iron Phenanthroline 0 6mg L 6003 Program No Nitrate Cd Reduction Chromotrophic Acid 0 1 5mg L 6903 C6903 FXD Cd Reduction Chromotrophic Acid 0 3mg L 6923 C6923 FXD AM Issue 3b 07 2001 English 59 60 AQUAMATE METHODS DISKS This page is intentionally left blank English AM Issue 3b 07 2001 CELL PROGRAMMER CELL PROGRAMMER Q The 7 Cell Programmer accessory enables up to seven samples to be presented for measurement sequentially Q This section describes the operation of the accessory with the software and also describes its removal refit When the Cell Programmer is installed then a status box appears on the top right hand side of the screen to the left of the Absorbance Wavelength box on all the pages except HOME
25. However the values of these factors may be affected by local conditions We recommend that in all cases the factors are checked with standard solutions appropriate to the laboratory and the equation modified accordingly O It should be noted that timers are not incorporated into the Dr Lange methods programmes These may be easily added if desired by referring to the TIMERS section of the FIXED applications pages in this manual Disk 4 CHEMetrics Vacu Vial amp Methods Q All CHEMetrics Vacu Vial Methods are FXD files These files have the following format Cxxxx FXD Q Once the relevant method has been loaded the FIXED page is displayed The file name appears at the top of the screen Operation 1 The sample preparation should be carried out according to the instructions supplied with the test kit 2 Prepare the blank following the instructions supplied with the test kit Place it into the cell holder and press the ZERO BASE key The instrument will then zero against the blank 3 Insert the prepared sample into the cell holder and press the RUN key A measurement will be carried out and the FIXED RESULTS page will be displayed 4 From the FIXED RESULTS page additional samples can be measured by inserting the sample and pressing the RUN key O Inall methods the relationship between absorbance and concentration is linear and takes the general form C A x FACTOR INTERCEPT Q Therefore the uvcalc equation typically takes th
26. IR Microcast uView Molecular Micro Mapping Momentum MonitIR Mosaic Nexus NicALAS Nicolet Nicolet Compact Four Nicolet Pathfinder Nic Plan NXR NYSTAR OMNI Sampler OMNI Transmission OMNIC Passport Performer Phoenix PLS Quant PLUS ProfilIR Progressive Sampling PROSTAR Prot g QC Complete QCIRCLE Quadrascan QuantPad Quick IR ReactIR Recorder Redundant Aperturing Reflachromat Reflex REGA RESULT RSVP SabIR SAGA Sample Plan Satellite SeaPort Si Carb SID Simul View Slide ON ATR Smart Accessory Smart ARK Smart Collector Smart Purge Snap In Solid Substrate SpecID SpecID Plus Spectra Bench Spectra Fax Spectra Scope SpectroPro SpeculATR SPECTRONIC SPIRIT SST Swap Top SX Sync TACT Talon Targeting Thermo Nicolet Corporation Thunderdome Total Coverage TQ Analyst Triton TruView TVF UpDRIFT Validate Validator ValPro Val Q Val Q DS Vectra Vectra Plus ViewThru Viking Viking II VISION ife VISIONIite VISIONpro VISIONsecurity WinFIRST WinFIRST Lite and WinSpec are trademarks of Thermo Electron Scientific Instruments Corporation a subsidiary of Thermo Electron Corporation r AQ AMAA E i User Manual Issue 3b 010718 Thermo Spectronic Europe Thermo Spectronic North America Middle East and Africa and Latin America Mercers Row Cambridge CB5 8HY UK 820 Linden Avenue Rochester NY 14625 Tel 44 0 1223 446600 USA Fax 44 0 1223 446644 Tel 800 654 9955 or 7
27. Q Identify the position of the keyway on the motor shaft Q View the underside of the basket to locate the position of the moulded key a Re locate the basket on the shaft ensuring a positive location of the key in the keyway and tightened the central screw clockwise Q From the CVC page use the INITIALISE function key to correctly identify the carousel with the spectrophotometer At this point check that the two serial numbers match CVC HOME Page CVC TEST I I I I I I TEST STATUS TIME DATE 1 WAVELENGTH PASS 11 05 03 12 96 2 ABSORBANCE PASS 11 20 03 12 96 i i 3 UV ABSORBANCE PASS 11 25 03 12 96 i 4 STRAY LIGHT PASS 11 28 03 12 96 i 5 BANDWIDTH PASS 11 33 03 12 96 H 6 NOISE PASS 11 38 03 12 96 i 7 DRIFT PASS 12 40 03 12 96 1 The UV Absorbance and Bandwidth tests are not available in AquaMateVis Q This page is reached by pressing CAL VAL from the HOME page Q This page lists the available tests and for each test reports the time and date on which the last test was run and whether it passed or failed Q To perform a test highlight the required option s either individually using the Arrow keys or as a group using the appropriate function keys and press RUN The instrument and lamp hours and energies are calculated and displayed on the appropriate page as each test is run 70 English AM Issue 3b 07 2001 CVC FUNCTION KEYS SAVE RESULTS Goes to the SAVE page from
28. TM measuring cylinder filled to the highest NO SIPS DONE t5 gradation to the sipper uptake tube and press the switch plate The sipper will pump a sample and the spectrophotometer will issue a beep Withdraw the measuring cylinder and the sipper will pump the air gap The values used for sample volume and air gap are those set on the SIPPER page Q Repeat this process for a number of cycles up to a maximum of 10 then press ENTER Q Measure the total volume taken from the measuring cylinder and enter this The calibration will be displayed FUNCTION KEY SIPPER PAGE Returns to the SIPPER page and abandons the calibration SIPPER CALIBRATION Page MOOR ee ee ee eee Q This page displays the current sipper i SIPPER CALIBRATION i sn pay PP 25 10 96 16 47 i NOMINAL VOL 1 000 ml Q To alter the calibration press CALIBRATE NO SIPS DONE 5 i TOTAL VOL SIPPED 5 100 ml i TUBING CAL 1 020 FUNCTIONS KEYS SIPPER PAGE Returns to the SIPPER page CALIBRATE Starts the calibration procedure AM Issue 3b 07 2001 English 65 MINISIPPER MINISIPPER Q The MiniSipper is an optional accessory that enables samples to be drawn into a flowcell of the user s choice for automatic measurement After the measurement is complete the sample is sent to Waste A continuous pumping mode allows the system to be washed through when required e g between applications This section describes the operation of th
29. The FXD files have the following format Hxxxx FXD where xxxx Hach Program Number FXD FIXED application in software Q Once the relevant FXD method has been loaded the FIXED page is displayed The file name appears at the top of the screen O If the Hach method indicates that timers are required then a number between 1 and 4 will be seen next to the TIMER S option on the FIXED page Operation 1 The Hach procedure should be followed until the first timer is required In order to start the first timer press the RUN key The screen will show the action to be carried out and the time remaining i e SHAKE REMAINING TIME 02 46 2 The instrument will beep to indicate the end of the time period and a TIMER FINISHED menu box will appear with the following options PROCEED ZERO STOP 3 In order to start the next timer select PROCEED using the arrow keys and press ENTER The next timer will begin At the end of this timer the same TIMER FINISHED menu box will appear 4 If the method procedure requires that a zero measurement be taken insert the blank select the ZERO option using the arrow keys and press ENTER A zero measurement will be taken and a menu box will appear with options to PROCEED or STOP 5 To take a measurement insert the sample into the cell holder and select PROCEED A sample measurement will be carried out and the FIXED RESULTS page is displayed 44 English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS Fr
30. desired wavelengths select PEAK TABLE and press ENTER then select PEAK HEIGHT A pop up box appears in which to enter the wavelengths required A and A define the baseline A defines the peak Once all the parameters have been set save the method 18 English AM Issue 3b 07 2001 SCAN GRAPH HIGH Select from range GRAPH LOW 0 01 to 6 00 Sets the upper graph limits on the SCAN GRAPH page GRAPH HIGH must be 0 01 greater than GRAPH LOW GRAPH LOW f Select from range 0 3 to GRAPH HIGH 0 01 Sets the lower graph limits on the SCAN GRAPH page GRAPH LOW must be 0 01 less than GRAPH HIGH CELL PROG Appears when the Cell Programmer is fitted Switches to the CELL PROG screen REF MODE Appears when the Cell Programmer is fitted Toggles the status of the Cell Programmer reference mode SCAN PARAMETERS page function keys SCAN VIEW RESULTS Switches to the Scan Peak Table screen if any of the peak functions have been performed or the Track Table screen if track has been used VIEW GRAPH Switches to the Scan Graph screen SAVE METHOD Brings up the Filename Function screen and then saves the method including ID and track wavelengths if the PEAK TABLE parameter is set to TRACK AM Issue 3b 07 2001 English 19 SCAN SCAN GRAPH Page O This page displays spectra and allows them to be manipulated L VIEW RESULTS Switches to the PEAK TABLE page SCAN PAGE Returns to the
31. from the file menu In the dialogue that appears type in AquaMate for the name and choose an icon Key OK In the next dialogue box select Direct to COM1 and key OK Fill in the COM 1 properties dialogue box as shown below Comat Fropiiteta Then from the FILE menu select PROPERTIES and then click on the SETTINGS tab Fill in the dialogue box as shown below Click on OK 80 English AM Issue 3b 07 2001 FAULT FINDING Helios Properties onnecting or disconnecting CONNECTING AQUAMATE TO A PC 1 Usea null modem cable part number 4013 172 82111 to connect the RS232C port of AquaMate to a free COM port on the PC Here is the pin out data for the RS232 Cable Pin Pin 9 to 9 2 3 3 2 4 8 8 4 6 1and7 1and76 5 5 Earth Earth Switch on the PC and set up a terminal programme as described above COLLECTING DATA Once you have established control of the instrument and switched on the debug software you will need to collect the data that is returned and save it to file This can be done by several methods the next section details one of them AM Issue 3b 07 2001 English 81 FAULT FINDING USING HYPERTERMINAL TO COLLECT DATA 1 From the menu bar select TRANSFERS and then CAPTURE TEXT Helios HyperT erminal Capture Text 2 The CAPTURE TEXT dialogue box appears Type in a path and file name then key START Capture Text 3 Oncethe instrument has stopped sending data from the
32. from the flowcell SAMPLE VOL SAMPLE WASTE RETURN LOW VOL OFF ON FUNCTION KEYS Enter a value between 0 2 and 9 999 ml Sets the volume of sample to be pumped Selects from WASTE or RETURN After measurement the sample is pumped through the flowcell to waste by the act of pumping the next sample After measurement the pump direction is reversed and the sample is returned to the sample vessel Toggles ON or OFF Automatically adjusts the pumping time to maintain the correct air gap for narrow uptake tubing Use standard internal diameter 1 1mm uptake tube Use narrow internal diameter 0 8mm uptake tube VIEW CALIB Goes to the SIPPER CALIBRATION page CALIBRATE Starts the Sipper calibration procedure 64 English AM Issue 3b 07 2001 SUPERSIPPER Sipper Calibration Q This calibrates the SuperSipper to take account of variations in pump and uptake tubing and sample viscosities A volume is set and using the appropriate solvent and tubing several sips are performed The actual volume sipped is entered and a calculation done to produce a calibration factor This factor is then used to adjust the pumping time to ensure that the correct volume of sample is always used Q Details of the calibration used are displayed on the SIPPER CALIBRATION page CALIBRATE SIPPER Page CALIBRATE SIPPER Q Using the solvent and tubing which will be used for the sample solutions offer a I NOMINA VOT
33. peaks Move the cursor to one of the options PEAKS and press ENTER to perform a search When the search is RADO complete the spectrum is displayed with the peak positions CORR RATIO PK HEIGHT marked For a peak to be found there must be more than 15 data points between that point and a previous peak For RATIO and CORR RATIO enter the wavelengths as prompted All results can be viewed by pressing OIOIO O view RESULTS PEAKS Marks the 10 highest peaks RATIO Calculates the ratio 2 4 CORR RATIO Calculates the ratio A A3 A2 A3 PK HEIGHT Calculates the peak maximum relative to a local baseline ORIGINAL Q This removes any manipulation and displays the spectrum as originally collected and specified by the scan method TRACK TABLE Page C CRACK LIST O The list shows the y axis values of the spectrum for the MARK ABS A WAVELENGTH nm wavelengths marked during TRACK The measured values sn Li will be ABS T or INTENSITY depending on the current 2 2 002 463 0 mode O VIEW GRAPH Switches to the TRACK page PRINT LIST Prints the list on the selected printer CHART OUTPUT Plots the spectrum on an analogue chart recorder SCAN GRAPH Returns to the SCAN GRAPH page 22 English AM Issue 3b 07 2001 PEAK TABLE PAGE PEAK TABLE ABS A WAVELENGTH nm 1 PEAK 0 472 447 0 2 PEAK 2 002 463 0 O O O RATIO TABLE Page RATIO TABLE
34. where the current set of results can be saved to Library or Disk Files are saved with a TST extension PRINT SUMMARY Prints the summary of results as shown on the page PRINT ALL Prints the summary of results as shown on the page and full details of the results of each of the tests TESTS 1 3 AquaMate only Selects the first three tests in the list These will be run in sequence when RUN is pressed TESTS 1 2 AquaMate Vis only Selects the first two tests in the list These will be run in sequence when RUN is pressed ALL TESTS Selects all the tests in the list These will be run in sequence when RUN is pressed Every time a test is run the carousel serial number is read and recorded on the results If this does not match the Calibration Data then the error E3083 Serial Numbers do not match is reported RESULTS Pages Q Each results page fully details the test performed the actual and measured values the differences tolerances etc as appropriate the spectrophotometer and CVC serial numbers the instrument hours and Tungsten lamp hours and energy From each Test Page the following functions are available FUNCTION KEYS TEST PAGE Returns to the TEST HOME page SAVE RESULTS Goes to the SAVE page from where the results can be saved to Disk PRINT RESULT Prints the result of the test STOP Stops a test Only present whilst a test is running If STOP is pressed then any results obtained f
35. 1 UVCALC 0 I CELL PROG AUTO l REF MODE ouer T rile OWE ini 1 MODE Select from ABS and T ABS Selects Absorbance T Selects transmittance ID f Enter a description using the TEXT ENTRY screen Q The ID identifies the method and unless changed will be saved with the method and any results produced by the method SELECT Select from SINGLE X MULTI A SERIAL A SINGLE This option is used to measure each sample at a single wavelength which is the same for each sample MULTI A This option allows each sample to be measured at up to 20 wavelengths which are the same for each sample SERIAL A This option allows a single wavelength measurement to be made ata different wavelength on up to 9 samples WAVELENGTH S SINGLE Use the numeric key pad to enter the required wavelength into the pop up box Press ENTER when finished AM Issue 3b 07 2001 English FIXED MULTI Use the up and down arrow keys to move to the wavelength to be entered or edited and press ENTER to display the edit box Use the numeric keypad to enter the wavelength and press ENTER when finished The instrument returns to the MULTI A screen with the next wavelength in the list highlighted Up to 20 wavelengths may be entered When the list is finished press the ACCEPT function key to accept the new list or the CANCEL function key to return to the FIXED METHOD page without changing the wavelength list SERIAL 7 Press ENTER to display the edit box
36. 16 248 4000 E mail info thermospectronic co uk Fax 716 248 4014 www thermospectronic com E mail info thermospectronic com www thermospectronic com Thermo Spectronic For Indian Sub continent Far East and Australasia Contact Thermo Spectronic Europe Middle East and Africa A Thermo Electron business USER MANUAL CONTENTS Navigate through this document by scrolling or click on a section title to move to that section directly Section Page Unpacking locating 3 1 Making connections Initialising eneee 1 SAFETY Safety notes Mia hill 3 ser Interface ec enn 4 ISAAC lads iaia 5 Data Store e sisisi snanar a ia i iaia 6 Quant Method LL aa iii eee 7 Quant Standards e etd redde dede 10 Quant Calibration i 11 Quant Results enne 12 Fixed Method ais eet eati eid e cete doa 13 Fixed Results essent 16 Scan Method eiie pereat dye nudes 17 ESI 20 Specification enn kainai niana ne nd Que e Lex nh dun ae eua 24 Operation 25 ule Ero 26 Error Messages rioni bu etes iners 29 B gr mE 30 DISK Drive ome ia aironi ni 32 Overview Clock and Printers 33 Environtnient uice cete etiem pedi s aa 34 Optical Initialisation aeee neet nns eese renr tnnseesrrnr nn 38 White Light Setup CVC 38 Recorder iubeo un a elus 40 E ce ER 41 ayireTo Ue fo REPERI 42 Set Up Procedure
37. 75 MAINTENANCE OA Hold the W lamp and pull upwards to remove When fitting the new tungsten halogen lamp avoid handling the silica envelope Finger marks become burnt on and cannot be removed after the lamp is switched on As this can affect the output characteristics handle only the base of the lamp If the silica envelope does become contaminated clean with a powerful degreasing solvent such as absolute alcohol before the lamp is switched on Q Use the new lamp s protective sleeve a polythene bag or a piece of tissue paper wrapped around the lamp and insert the pins into the socket a Replace the spring clip These lamps are manufactured to very high tolerances but to ensure optimum energy throughput align the lamp filament exactly as shown in the diagram with the white line on the lamp base facing towards the front of the instrument O Refit both the metal and rear covers Q Reconnect the spectrophotometer to the mains supply and switch on Q Lamp hours and energy must be reset from the controlling software REMOVAL AND REPLACEMENT OF DEUTERIUM LAMP not AquaMate Vis WARNING 1 Switch off and disconnect the spectrophotometer from the mains supply and allow the lamp to cool for at least 15 minutes before proceeding 2 UV radiation from a Deuterium lamp can be harmful to the skin and eyes Always view the lamp through protective glasses that will absorb UV radiation Avoid looking directly at the Deuterium ar
38. ACCEPT Numbers are entered using the numeric keypad If you make a mistake OJOIOIOIe will act as a backspace or CLEAR will clear the whole entry Filenames are limited to 8 characters Q To change the FILE TYPE highlight the field and press ENTER to display the pop up menu Available formats are NORMAL the native file type of the Local Control Software and CSV Comma separated variable Highlight your choice and press ENTER to select The ID field will contain the characters entered for the method To edit the ID highlight the ID field and press ENTER change as necessary then press ACCEPT To select the destination of the file highlight the DRIVE field Pressing ENTER will toggle between LIBRARY and DISK lt Acts as backspace and clears the last character in the line ACCEPT SAVE ACCEPT if edit is in progress Accepts the edited field ready for saving SAVE not present during an edit saves file to Library or Disk CANCEL Abandons the naming operation No files will be changed and the software returns to the calling screen English AM Issue 3b 07 2001 QUANT QUANT Instrument and analysis parameters are set up on the QUANT page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q Once all results have been collected save the data QUANT METHOD Page I QUANT 1 I I I ID i W
39. AVELENGTH 550 0 nm I BANDWIDTH 2 0 nm i INTEGRATION 1s i STANDARDS 3 i REPLICATES 1 UNITS CURVE FIT LINEAR MEASURE STDS YES TIMER S 0 LAMP CHANGE 325 nm l OPERATOR UVCALC 0 CELL PROG AUTO REF MODE 315 RA f ID Enter a description using the TEXT ENTRY screen Q The ID identifies the method and will be saved with the method and any results produced by the method WAVELENGTH A Select a wavelength between 190 0 nm or 315 0nm for AquaMate Vis and 1100 0 nm If the wavelength requires the Deuterium lamp if fitted then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH 3 This is fixed at 2 0 nm INTEGRATION Enter integration time in seconds This sets the integration time for which the result is measured The current data will be lost if the integration time is changed STANDARDS Brings up the Standards Entry Page Use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display the Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry page with the highlight on the next standard on the list Up to 20 standards can be specified Changing the standards will cause any current data to be lost AM Issue 3b 07 2001 English 7 QUANT
40. BRATION calibration 25 08 99 16 47 NOMINAL VOL sai i Q To alter the calibration press CALIBRATE NO SIPS DONE 5 TOTAL VOL SIPPED 5 100 ml l TUBING CAL 1 020 1 FUNCTIONS KEYS SIPPER PAGE Returns to the SIPPER page CALIBRATE Starts the calibration procedure 68 English AM Issue 3b 07 2001 CVC CALIBRATION VALIDATION CAROUSEL CVC Q The Calibration Validation Carousel replaces the standard cell basket and allows for the automatic checking of the spectrophotometer to specification by measurement of the fundamental operating parameters THE PROCESS OF CALIBRATION OF ITS WAVELENGTH AND ABSORBANCE FILTERS IS ACCREDITED BY THE UNITED KINGDOM ACCREDITATION SERVICE UKAS TO AN ISO IEC GUIDE 25 APPROVED PROCEDURE Q Calibration can be performed automatically on start up if the CVC is fitted From the SETUP menu select ENVIRONMENT select AUTOMATIC CAL VAL and toggle to ON with the Enter key On start up the instrument will then automatically wait for the warm up period 60 minutes and then perform tests 1 2 and 3 tests 1 and 2 in AquaMate Vis Calibration can be aborted by pressing Clear Q Calibration values are provided on a PC format floppy disk which is loaded on installation see below IT 1S RECOMMENDED THAT A BACK UP COPY OF THIS DISK IS MADE BEFORE USE AND THE MASTER RETAINED IN A SECURE SAFE LOCATION Q This section describes the installation and operation of th
41. D 0 015 2 0 mg l NH4 N 1 3 15 0 mg l NH4 11mm round LCK 305 K305CT FXD E CES SN LOS UN 2 5 60 0 mg l NH4 11mm round LCK 303 K303CT FXD Er M ED e RENT 60 167 mg l NH4 11mm round LCK 302 K302CT FXD EXER SE I RT 0 23 13 50 mg l NOs N 5 35 mg l NO3 N 0 015 0 6 mg l NO2 N 0 005 0 100 mg l NO 50mm Rectangular LCK 341 K341P50 FXD 0 002 0 030 mg l NO2 N 2 20 mg l 11mm round LCK 342 K342CT FXD 0 6 6 0 mg l Nitrogen Total Kjeldahl 1 10 mg l mg l TKN 11mm round LCW909 W909CT FXD 10 200 mg l mg l TKN 200 2000 mg l mg l TKN Phenols Organic Complexing 3 20 mg l NTA LCW907 W907CT FXD Agents 5 90 mg l PO4 11mm round LCK 049 K049CT FXD 1 6 30 0 mg l PO4 P 3 7 70 0 mg l P205 AM Issue 3b 07 2001 English 57 AQUAMATE METHODS DISKS Lange Disk 3 Analyte Range Units Cell Program no AquaMate File no Phosphorus Total 0 01 0 50 mg l PO4 P 50mm Rectangular LCK349 K349P50 FXD 0 03 1 50 mg l PO 0 02 1 20 mg l P205 0 05 1 50 mg l PO4 P 11mm round LCK 349 K349CT FXD 0 15 4 50 mg l PO 0 15 3 50 mg l P205 0 5 5 0 mg l PO4 P 11mm round LCK 348 K348CT FXD 1 5 15 0 mg l PO4 1 2 11 5 mg l P205 2 20 mg l PO4 P 11mm round LCK 350 K350CT FXD 6 60 mg l 4 5 45 0 mg l 5 50 mol eK as KETO Silver 5 400 mg l 11mm round LCK 355 K355CT FXD 400 2500 mg l Si Sulphate 40 150 mg l LCK 153 K153CT FXD ulphide 0 1 2 0 mg l 10mm Rectangular LCW 053 W053P10
42. D DISKS Cell Type Units Program AquaMate File no 0 25 1 50 mg l 20mm Rectangular mg l Ag 14831 14831P20 FXD Sulpha Sulphite 0 05 3 00 mg l 50mm Rectangular mg l SO3 14394 14394P50 FXD 0 5 15 0 mg l 20mm Rectangular mg l S037 14394 14394P20 FXD 1 0 25 0 mg l 10mm Rectangular mg l S037 14394 14394P10 FXD 1 0 25 0 mg l 16mm Round mg l SOs 14394 14394P16 FXD Surfactants 0 05 2 0 mg l 16mm Round mg l MBAS 14697 14697P16 FXD Determination 0 10 2 50 mg l 16mm Round mg l Sn 14622 14622P16 FXD inc 5 250 mg l 10mm Rectangular mg l SO4 14548 14548P10 FXD 0 50 3 00 mg l 10mm Rectangular mg l Ag 14831 14831P10 FXD te 5 250 mg l 16mm Round mg l SO4 14548 14548P16 FXD 5 250 mg l 20mm Rectangular mg l SO 14548 14548P20 FXD 0 10 1 50 mg l 20mm Rectangular mg l Sn 14622 14622P20 FXD 0 050 0 500 mg l 50mm Rectangular mg l Zn 14566 14566P50 FXD 0 10 2 50 mg l 10mm Rectangular mg l Sn 14622 14622P10 FXD AM Issue 3b 07 2001 English 53 AQUAMATE METHODS DISKS Analyte CEC Method Range _ Program no AquaMate File no Aluminium Barium Boron Bromine IDPD____ 0 450mgi 1300 H1300FXD H1350 QNT 0 25 0 mg l H1400 QNT Chlorine Free H1450 FXD H1460 FXD DPD 0 500mgl 1470 H1470FXD H1480 FXD Chlorine Total DPD X J 0 2 00mgi 1450 H1450FXD H1460 FXD H1470 FXD
43. DING Fault Finding Guide Problem Symptom Pos sible Cause Instrument dead The fans are not running 1 N ooo Ensure the mains lead is firmly pressed home Some leads are a very tight fit in the three pin IEC connector on the rear of the instrument If a switched outlet is being used ensure it is on Check the fuse in the plug Try another mains lead Try another mains outlet Ensure the power switch on the instrument has been fully operated If all of the above are OK the power supply may have failed Contact your local agent Display Blank No text on display a slight glow can be Seen around the edges of the display in subdued lighting The contrast control is incorrectly set Go to the HOME page the right and left arrow keys adjust the contrast If nothing happens it may be that error messages are being displayed Press the CLEAR key 5 times at 10 second intervals to clear the error list and try again The display contrast is affected by temperature and may need adjusting from day to day or as the instrument warms up The instrument has been put into remote control without allowing it to initialise first The display will return next time that the instrument is switched on An unsuccessful attempt has been made to update the software Connect the instrument to a PC and repeat the software upgrade process Any performance problem Failure to initialise or error 1053 N
44. ER MODE An invalid cell programmer mode has been selected Check settings AM Issue 3b 07 2001 English 29 LIBRARY LIBRARY Page Q To access the Library select LIBRARY on the HOME page and press ENTER The library is where all the method and data files are stored and consists of two areas the instrument library and the disk drive From the library files can be loaded renamed copied to or from the disk drive and deleted Saving files to the library is done from the method and result pages of the application LIBRARY TYPE ID FILE NAME M QUANT UV123 AB123B QNT M FIXED UV146 DE146G FXD D QUANT UV146 TEST QNT D FIXED UV146 TEST2 FXD M FIXED IX2 THRIB FXD 76 LIBRARY SPACE REMAINING HIGHLIGHT FILE AND PRESS ENTER PRINT FORMAT COPY VIEW DIR LIBRARY ALL DISK Q The LIBRARY Page lists all the files in the Library including the file type description and file name FILE TYPE This describes the type of information contained in the file M QUANT Quant method including calibration D QUANT Quant results including method and calibration M FIXED Fixed wavelength method D FIXED Fixed wavelength results and method M SCAN Scan method DESCRIPTION This is the description entered when the file was saved and corresponds to the Method ID FILENAME This is the DOS compatible filename used by the instrument and a computer 30 English AM Issue 3b 07 2001 LIBRARY LIBRARY 290000 VIEW DISK
45. ERS RETAINED IN A SECURE SAFE LOCATION With this procedure the small number of method files required for a specific laboratory environment are copied from one or more of the methods disks on to another disk These methods can either be run directly from this disk or saved to the LIBRARY and run from there Whilst not essential to the use of the software this procedure will allow for the production of a Methods Disk that is User specific and may contain files from any of the three methods disks It will also allow fast access to any required method from the DISK or LIBRARY options on the HOME page From the REFERENCE section identify the required FXD or QNT file or files On a PC using the back up copy of the chosen method disk and any appropriate File Management software e g File Manager Explorer etc copy these files onto a formatted 3 HD disk and label the disk Methods Disk These methods can then be used by selecting the DISK option from the HOME page highlighting the chosen method using the arrow keys and pressing ENTER A menu box will appear with the following options LOAD RENAME SAVE TO LIBRARY DELETE English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS To run the method from the disk select LOAD and press ENTER To save the method to the LIBRARY select SAVE TO LIBRARY The 20 preinstalled methods found in the library can be deleted to make space for the user s preferred methods These 20 methods can
46. LOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE MEASURING ITS ENERGY CLEAR BOTH SAMPLE AND REFERENCE BEAMS BEFORE MEASURING LAMP ENERGIES W ENERGY ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE MEASURING ITS ENERGY CLEAR BOTH SAMPLE AND REFERENCE BEAMS BEFORE MEASURING LAMP ENERGIES SWITCH D2 Turns the Deuterium lamp ON or OFF depending on its current status English AM Issue 3b 07 2001 SETUP LAMPS Page AquaMate Vis Q The lamp functions are available directly from the LAMPS key on the SETUP page If User Log on is not in use the HOME page will also have a LAMPS key Q This page shows the status of the Tungsten Halogen lamp whether ON OFF or FAILED and its energy level It also allows the lamp hours to be reset Q The HOURS parameter states the number of hours that the lamp has been in use The Tungsten Halogen lamp should be replaced after 2000 hours Whenever a lamp is changed then the hours parameter should be reset to zero LAMPS TUNGSTEN HOURS ENERGY OIOIQIOI RESET W HRS Resets hours and measures energy at the appropriate wavelength ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE RESETTING ITS HOURS W ENERGY ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE MEASURING ITS ENERGY CLEAR BOTH SAMPLE AND REFERENCE BEAMS BEFORE MEASURING LAMP ENERGIES AM Issue 3b 07 2001 English 41 AQUAMATE METHODS DISKS AQUAMATE METHOD DISKS INTRODUCTION a Water an
47. M Issue 3b 07 2001 AQUAMATE METHOD DISKS L Merk Diski TTT nale Range Gell type Units Program AquaMate File no 0 05 0 75 mg l 20mm Rectangular 14825P20 FXD 14825P50 FXD 14839P 10 FXD i 14834P16 FXD 14834P20 FXD 14834P10 FXD 14834P50 FXD 14815P20 FXD 10 160 mg l 10mm Rectangular mg l Ca 14815 14815P10 FXD 1 0 15 0 mg l mg l Ca 5 125 mg l 16mm Round mg l CI 14730 14730P16 FXD 5 125 mg l 20mm Rectangular mg l CI 14730 14730P20 FXD 5 125 mg l 10mm Rectangular mg l CI 14730 14730P10 FXD 0 01 1 50 mg l 50mm Rectangular mg l Cla 14828 14828P50 FXD 0 05 4 00 mg l 20mm Rectangular mg l Cla 14828 14828P20 FXD 0 10 7 50 mg l Omm Rectangular mg l Cla 14828 14828P10 FXD 1 Chlorine Chlorine 0 01 1 00 mg l 50mm Rectangular C 14732 14732P50 FXD Dioxide amp Ozone 0 02 1 00 mg l 0 01 1 00 mg l 0 05 2 50 mg l 20mm Rectangular 14732 14732P20 FXD 0 05 2 50 mg l 0 05 2 50 mg l 0 10 5 00 mg l 10mm Rectangular 14732 14732P10 FXD 0 10 5 00 mg l 0 10 5 00 mg l 0 05 2 00 mg l 16mm Round mg l Cr 14552P16 FXD 14562P20 FXD 14552P10FXD 14758P50FXD 14758P20FXD 14758P10 FXD COD Oxygen 4 0 40 0 mg l 16mm Round mg l COD 14560 14560P16 FXD Demand Chemical PEPO 14895P16 FXD 14690P16 FXD 14541P16 FXD 14691P 16 PXD T4555P16 FXD 14553P16 FXD 14553P20 FXD 14553P 10 FXD 14767P50 FXD 14767P20 FXD 14767P 10 FXD 14561P16 EXD 14561P20 EXD
48. Mate File no Nitrogen Nitrite 0 020 0 610 mg l 16mm Round mgl NO N 14547 14547P16 FXD 0 05 2 00 mg l mg l NOz 0 010 0 500 mg l 20mm Rectangular mg l NOzN 14547 14547P20 FXD 0 03 1 60 mg l mg l NO7 0 020 1 000 mg l 10mm Rectangular mg l NOzN 14547 14546P10 FXD 0 100 3 00 mg l mg l NOz 0 005 0 200 mg l 50mm Rectangular mg l NO2 N 14776 14776P50 FXD 0 015 0 650 mg l mg l NO2 0 010 0 500 mg l 20mm Rectangular mg l NO2 N 14776 14776P20 FXD 0 03 1 60 mg l mg l NOz 0 02 1 00 mg l 10mm Rectangular mg l NO2 N 14776 14776P10 FXD 0 10 3 00 mg l mg l NOz Nitrogen Total 0 5 15 0 mg l 16mm Round mg l N 14537 14537P16 FXD 0 3 10 0 mg l 20mm Rectangular mg l N 14537 14537P20 FXD 1 14763P10 FXD Onygen Dissolved 14094P16 FXD TA094P20 FXD 14604P 10 FXD 0 5 15 0 mg l Omm Rectangular mg l N 14537 14537P10 FXD 10 150 mg l 16mm Round mg l N 14763 14763P16 FXD 0 10 2 50 mg l 16mm Round mg l phenol 14551 14551P16 FXD 0 025 1 000 mg l 50mm Rectangular mg l phenol 14551 14551P50 FXD 0 10 2 50 mg l 20mm Rectangular mg l phenol 14551 14551P20 FXD 0 10 2 50 mg l 10mm Rectangular mg l phenol 14551 14551P10 FXD Phosphorus PMB 0 01 1 00 mg l 50mm Rectangular mg l PO4 P 14848 14848P50 FXD 0 05 3 00 mg l mg l PO4 0 03 2 50 mg l 20mm Rectangular mg l PO4 P 14848 14848P20 FXD 0 10 7 50 mgll mg l PO4 0 05 5 00 mg l
49. Now navigate to your chosen method insert a blank sample and press ZERO Once the instrument has finished zeroing adjust the chart recorder backoff so that its baseline is at the required position Q N B The chart recorder will be driven off scale while the instrument is zeroing AM Issue 3b 07 2001 English 39 LAMPS Page AquaMate SETUP Q The lamp functions are available directly from the LAMPS key on the SETUP page If User Log on is not in use the HOME page will also have a LAMPS key Q This page shows the status of the Tungsten Halogen and Deuterium lamps whether ON OFF or FAILED and their appropriate energy levels It also allows the lamp hours to be reset and the Deuterium lamp to be switched on or off Q The HOURS parameter states the number of hours that the lamp has been in use The Tungsten Halogen lamp should be replaced after 2000 hours The Deuterium lamp should be replaced after 1000 hours Whenever a lamp is changed then the hours parameter should be reset to zero LAMPS TUNGSTEN HOURS ENERGY D2 HOURS ENERGY 40 ON 987 9996 STRIKING 234 9696 RESET W HRS Resets hours and measures energy at the appropriate wavelength ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE RESETTING ITS HOURS RESET D2 HRS Resets hours and measures energy at the appropriate wavelength ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE RESETTING ITS HOURS W ENERGY AL
50. RDER Page RECORDER I I I I I I I I i i CHART HIGH ABS 3 000 CHART LOW ABS 0 300 I I CHART HIGH T 200 0 CHART LOW T 0 1 I I I I CHART HICH I 99 9999 CHART LOW I 0 0000 i i I I Ta jaa i n n n cp E a a aa a Q The Chart High and Chart Low parameters set the full scale deflection on the analogue chart recorder output for each of the available measurement modes On startup these limits are set to the maximum measurement ranges as shown above Q To reset the limits highlight the required parameter and press ENTER Enter the new value using the number keys and press ENTER Using a Chart Recorder Q Use the Recorder Lead part number 4401 172 00401 to connect the recorder to the socket labelled REC located to the rear of the spectrophotometer This lead is used for both 0 10mV and 0 1V full scale deflection fsd chart recorders Use the blue plug for 0 10mV or the red plug for 0 1V a NB If your chart recorder is capable of either voltage range it is advisable to use the 0 1V setting and only use the 2 appropriate plugs red and black Q The Chart High and Chart Low parameters set the 0 to 1V full scale deflection fsd on the analogue chart recorder output for each of the available measurement modes Q Operating tip if you are working with absorbances close to zero best results are obtained if the Chart Low ABS limit is set to a small negative value say 0 1A Q
51. SCAN page CHART OUTPUT Plots the spectrum on an analogue chart recorder PRINT GRAPH Provides a hardcopy of the results as shown on screen MANIPULATE Displays the Manipulate options Q Pressing RUN starts a scan using the current method Q Pressing ZERO BASE starts a baseline using the current method See page 5 MANIPULATE OPTIONS TRACK Reports x and y axis values using the TRACK tracking cursor RESCALE as RESCALE Changes x and y axis scales automatically ORIGINAL or manually MODE Changes mode Select from T or ABS PEAKS Finds spectral peaks Select from PEAKS RATIO CORR RATIO PK HEIGHT ORIGINAL Resets the graph to display the data as originally collected 20 English AM Issue 3b 07 2001 SCAN TRACK Q Tomovethe vertical cursor across the screen use the Left and Right Arrow keys The cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current wavelength Up to 10 wavelengths can be selected TRACK page function keys Q Pressing CLEAR will delete markers in turn highest number first Q The x axis values are listed on the TRACK table page Further markers can be added to the spectrum at any time 600 0 however selecting TRACK will cause any previous PEAK 500 0 TABLE information to be lost 400 0 VIEW TABLE Switches to the TRACK TABLE page FAST SLOW Toggles between two cursor speeds In
52. alysis using UV visible spectroscopy is built around two fundamental principles measurement of an analyte and subsequent calculations based on these measurements to yield results in terms of concentration In this day and age water analysis has advanced to such a degree that there are fully validated test kits available from leading suppliers that facilitate the practical aspects of performing water analyses The AquaMate system takes full advantage of the vast range of test kits from these sources not only through its ability to house the various cells cuvettes used but also by providing the methods required The AquaMate system comes with 20 methods programmed into its library The full range of methods are available on the four PC format floppy disks supplied Disk 1 provides the set ups and all defined equations for the Merck Spectroquant tests Disk 2 provides the methods for the Hach water analysis kits Disk 3 contains the methods for the Dr Lange cuvette and pipette test kits and disk 4 contains the methods for CHEMetrics Vacu Vials kits In addition to this the AquaMate software allows the user to modify or create new methods by entering formulae into its unique uvcalc software Further information on available methods and method formats etc can be found in the REFERENCE section at the end of this chapter SET UP PROCEDURE a 42 BEFORE BEGINNING IT IS RECOMMENDED THAT BACK UP COPIES OF THE METHODS DISKS ARE MADE AND THE MAST
53. angular mg l NH4 N 14739 mg l NH4 16mm Round mg l NH4 N 14558 mg l NH4 10mm Rectangular mg l NH4 N 14558 mg l NH4 16mm Round mg l NH4 N 14544 mg l NH4 10mm Rectangular mg l NH4 N 14544 mg l NH4 16mm Round mg l NH4 N 14559 mg l NH4 10mm Rectangular mg l NH4 N 14559 mg l NH4 50mm Rectangular mg l NH4 N 14752 mg l NH4 20mm Rectangular mg l NH4 N 14752 mg l NH4 10mm Rectangular mg l NH4 N 14752 mg l NH4 16mm Round mg l NO3 N 14556 mg l NO3 20mm Rectangular mg l NOs N 14556 mg l NO3 10mm Rectangular mg l NOs N 14556 mg l NO3 16mm Round mg l NO3 N 14764 mg l NO3 10mm Rectangular mg l NOs N 14764 mg l NO3 16mm Round mg l NO3 N 14542 mg l NO3 20mm Rectangular mg l NOs N 14542 mg l NO3 10mm Rectangular mg l NOs N 14542 mg l NO3 16mm Round mg l NO3 N 14563 mg l NO3 20mm Rectangular mg l NOs N 14563 mg l NO3 10mm Rectangular mg l NOs N 14563 mg l NO3 20mm Rectangular mg l NOs N 14773 mg l NO3 10mm Rectangular mg l NOs N 14773 mg l NO3 English AquaMate File no 14739P16 FXD 14739P10 FXD 14558P16 FXD 14558P10 FXD 14544P16 FXD 14544P10 FXD 14559P16 FXD 14559P10 FXD 14752P50 FXD 14752P20 FXD 14752P10 FXD 14556P16 FXD 14556P20 FXD 14556P10 FXD 14764P16 FXD 14764P10 FXD 14542P16 FXD 14542P20 FXD 14542P10 FXD 14563P16 FXD 14563P20 FXD 14563P10 FXD 14773P20 FXD 14773P10 FXD 51 AQUAMATE METHODS DISKS Cell Type Units Program Aqua
54. asurement of the parameter and subsequent calculations based on these measurements In UV Visible spectrophotometry and many other mature techniques the science associated with the measurement of the parameter is well developed to such a degree that there are fully validated test kits available from the leading chemical suppliers in the key areas of bio chemical and environmental water chemistry In addition most laboratories also have their own fully developed internal procedures In these days of instruments controlled by on board software it is some what surprising that the analyst at the bench may still have to manually use a calculator to perform a final calculation and then make an informed decision as to whether the result meets expected criteria At this point even the most experienced analyst can still make a transcription error mis interpret a written figure etc With defined procedures many standard methods will document the final calculation in the form of an algebraic formula Uvcalc allows these formulae to be entered in the software method together with the control limits Uvcalc provides automatic calculation of results from measurements using user defined equations The measurement is obtained from the spectrophotometer in the form of individual results in Fixed and Quant methods SPECIFICATION 24 Up to 4 different equations may be applied to each measurement The formula editor supports
55. ate on the same page until it is full O To move up or down pages of results use the Up Down arrow keys a Results are numbered sequentially from 1 to 600 FIXED RESULTS ABS ABS 1 0 201 15 2 0 201 16 CLEAR RESULTS All results are cleared ready for the start of the next batch FIXED PAGE Returns to the FIXED METHOD page SAVE DATA Brings up the SAVE page from where the results can be saved to Disk PRINT LIST Prints the list on the selected printer LIMS EXPORT Sends the results via the RS232 port Q Press RUN to take another sample measurement a Press ZERO BASE to zero the instrument at the wavelength s specified in the method See page 5 16 English AM Issue 3b 07 2001 SCAN SCAN Q To select Scan highlight the SCAN option on the HOME PAGE and press ENTER The SCAN Methods page is displayed and from here the instrument and analysis parameters can be set up Q Move the cursor to the required parameter using the Up Down Arrow keys Press ENTER to enable a parameter to be changed Q Once the method has been set up press ZERO BASE to perform a baseline scan with the current method see page 5 and then press RUN The spectrophotometer will perform the scan and display the result on the SCAN GRAPH page From here the spectrum can be manipulated and printed Q The scan speed is set to 600 nm per min and the data interval is 0 5nm SCAN PARAMETERS Page Note The current spect
56. aution statements and or symbols are marked on the apparatus where necessary The instrument covers and accessories should only be removed by personnel who have been trained to avoid the risk of electric shocks The mains electricity supply to the instrument must be disconnected at the mains supply connector and at least three minutes allowed for capacitors to discharge Some of the chemicals used in spectrophotometry are corrosive and or flammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals The UV radiation from a deuterium lamp can be harmful to the skin and eyes Always view the lamp through protective glasses goggles that will absorb the UV radiation and avoid looking directly at the deuterium arc Do not expose the skin to direct or reflected UV radiation Whenever it is likely that safety protection has been impaired the instrument and or accessory must be made inoperative and secured against any unintended operation The matter should then be referred to the appropriate servicing authority Safety protection is likely to be impaired if for example the instrument fails to perform the intended measurements or shows visible damage AM Issue 3b 07 2001 English 3 GENERAL GENERAL Introduction Q The range comprises a group of UV Visible and Visible spectrophotometers which can be controlled independently via an integ
57. be changed unless ACCEPT is pressed I I DAY 25 I MONTH 12 Q CANCEL cancels the edit leaving the previous values unchanged YEAR 96 I I PRINTERS Page Q This page sets the system to work with the selected printer Q The printer type is always highlighted If an internal printer is supplied this will be the default printer otherwise the default printer is HP Mono To choose a printer press ENTER to display the list of supported printers and select using cursor keys Press ENTER to confirm entry AM Issue 3b 07 2001 English 33 SETUP Printer options PRINTER EPSON 9 PIN EPSON 9 PIN FR SEIT HP LASERJET HP MONO HP MONO HP PLOTTER HP 690C HP PLOTTER HP 400 INTERNAL HP 690C HP 400 INTERNAL Supported Printers Epson 9 or 24 Pin Dot Matrix using ESC P language HP Laserjet Series HP Deskjet 500 Series and above Black amp White Compatible with plotters using HPGL language HP Deskjet 690C Colour HP Deskjet 400 Mono Supplied thermal printer only Printers not on the above list that claim Epson 9 pin 24 pin ESC P or HP PCL Programming Control Language Level 3 compatibility should work with the instrument but are not guaranteed to do so and are therefore not supported If in doubt contact Thermo Spectronic approved Customer Support Note Printers designed to work only in a Windows environment are not compatible with AquaMate Software Before attempting to print using an exte
58. be found on the Merck Hach methods disks should the user wish to use these methods in the future OPERATION Running files from a Methods Disk or the library 1 To run from the Methods Disk insert the disk into the disk drive select DISK from the HOME page Alternatively to run a file from the library select LIBRARY from the HOME page From the directory of methods shown on the screen use the arrow keys to highlight the desired method Pressing Enter Load will now load the method Running from Disk 1 Disk 2 Disk 3 or Disk 4 a Methods can be run directly from these disks but with between 70 and 180 files on each disk selection of the desired file may prove to be laborious Running Individual Methods Disk 1 Merck Spectroquant Methods a All Merck Spectroquant Methods are FXD files The majority of files have the following format 14xxxPyy FXD where 14xxx Merck Catalogue Number yy pathlength of cell in mm FXD FIXED application in software In some cases the Merck catalogue number is of the format 10xxxx In this case the AquaMate files have the following format OxxxxPyy FXD Once the relevant method has been loaded the FIXED page is displayed The file name appears at the top of the screen Operation 1 2 3 4 The sample preparation should be carried out according to the instructions supplied with the test kit When the blank has been prepared place it into the cell holder an
59. c Do not expose the skin to direct or reflected UV radiation Q Setthe power switch to off and disconnect the spectrophotometer from the mains supply a Remove the back corner cover by turning the fastener one quarter turn anti clockwise and sliding the cover up to remove 76 English AM Issue 3b 07 2001 MAINTENANCE Q Make sure the lamp has cooled disconnect the lamp at the in line Inline connector connector Using the key provided loosen the three locating screws rotate lamp assembly anti clockwise and lift lamp out When fitting the Deuterium lamp avoid handling the silica envelope Finger marks become burnt on and cannot be removed after the lamp is switched on This can affect the light output characteristics Handle only the base of the lamp or the mounting plate If the silica envelope becomes contaminated clean with a powerful degreasing solvent Mounting such as absolute alcohol before the lamp plate is switched on O Take the new lamp identify the notch in the mounting plate Locate the lamp such that the notch points towards the lamp change mirror Tighten locating screws down with the key provided Locating screws a Re connect the new lamp at the in line connector Q Refitthe rear cover Q Reconnect the spectrophotometer to the mains supply and switch on Allow half an hour for warm up time Q Lamp hours must be reset by the controlling software AM Issue 3b 07 2001 English 77 FAULT FIN
60. d press the ZERO BASE key The instrument will then zero against the blank Insert the prepared sample into the cell holder and press the RUN key A measurement will be carried out and the FIXED RESULTS page will be displayed From the FIXED RESULTS page additional samples can be measured by inserting the sample and pressing the RUN key AM Issue 3b 07 2001 English 43 AQUAMATE METHODS DISKS Q Inall but one method 14825P50 FXD the relationship between absorbance and concentration is linear over the specified measuring range and takes the general form C AxFACTOR O Therefore the uvcalc equation typically takes the following form Method 14566P16 FXD Zinc Equation M1 4 88 O The factors entered are those documented by Merck However the values of these factors may be affected by local conditions We recommend that in all cases the factors are checked with standard solutions appropriate to the laboratory and the equation modified accordingly Q It should be noted that timers are not incorporated into the Merck method programmes These may easily be added if desired by referring to the TIMERS section of the FIXED application pages of this manual Disk 2 Hach Test Kit Methods Q The Hach test kit methods are of two types either FXD files or QNT files Q Instructions for running the Hach methods on Aquamate are stored in PDF format on the accompanying CD together with a copy of Adobe Acrobat Reader version 5 FXD Files Q
61. e MiniSipper with the Local Control software Full details of the installation and operation of the accessory are described in the MiniSipper Installation and Maintenance Manual 9499 230 45111 supplied with the MiniSipper Q To operate the MiniSipper install as described in the above manual Present the sample to the MiniSipper and press the switch The required sample volume will be drawn into the tubing When the system beeps remove the sample and the required air gap will be drawn in Once the measurement has taken place press the switch again The sample will be pumped out of the flowcell to waste When the sipper is connected a status box is displayed on the right hand side of all the method screens that indicates the presence of the MiniSipper and its status SIPPER Page O This page allows the MiniSipper to be set up and press ENTER SIPPER i for the required analysis The method set on I f I this page will be saved with any data MODE 2 d produced by the software I AIR GAP 50 cm I SAMPLE VOL 1 000 ml i O To reach this page press ACCESSORIES SAMPLE C WASTE from the HOME page then select SIPPER LOW VOL OFF To change a parameter highlight the required value using the Up Down Arrow keys and press ENTER SIPPER Toggle ON or OFF Switches the MiniSipper ON or OFF MODE Selects from SIP SIP amp RUN CONTINUOUS AUTOMATIC SIP Sets the system to fill the flowcell SIP amp RUN Sets the sy
62. e accessory with the software Q This section also describes its removal refit SETUP CVC De et AI 1 SETUP CVC 1 Q This page allows the CVC calibration data i provided on disk to be loaded into the CALIBRATION DATA spectrophotometer memory To reach this LC CeRRIAIINUMBER ES page highlight the CVC option from the CALIBRATION DATE 03 12 96 SETUP page and press ENTER CAROUSEL SERIAL NUMBER 32764 PEER EE ERE EA MAFIA RIAPERTO 1 SERIAL NO Displays the serial number of the calibration and therefore the actual parameter values loaded into the memory of the spectrophotometer CALIBRATION DATE f Date of original calibration CAROUSEL SERIAL NO Unique identifier read by initialising the carousel FUNCTION KEYS LOAD DATA Allows calibration data to be loaded into the spectrophotometer INITIALISE Initialises the carousel and reads the serial number SETUP PAGE Returns back to SETUP AM Issue 3b 07 2001 English 69 CVC SETUP PROCEDURE Q From SETUP choose CVC and place the disk in the drive on the spectrophotometer The first time this procedure is actioned a warning message W1022 NVM Checksum will be displayed This is expected Press C to clear Q With the disk in place press LOAD DATA Appearance of the appropriate Serial Number and Calibration Date confirms storage of the data in NVM CAROUSEL INSTALLATION a Remove the cover from the optosensor
63. e following form Method C1603 FXD Bromine Equation M1 7 89 0 04 Q The FACTOR and INTERCEPT values have been determined by CHEMetrics specifically for Aquamate However the values of these factors may be affected by local conditions We recommend that in all cases the factors are checked with standard solutions appropriate to the laboratory and the equation modified accordingly Q It should be noted that timers are not incorporated into CHEMetrics methods programmes These may be easily added if desired by referring to the TIMERS section of the FIXED applications pages in this manual AM Issue 3b 07 2001 English 47 AQUAMATE METHODS DISKS FIXED RESULTS and QUANT RESULTS Pages Q The FIXED RESULTS and QUANT RESULTS pages clearly show the absorbance of the sample the concentration of the analyte PASS FAIL indicator Q The pass fail indicates whether the recorded concentration of the sample falls within the measurement range of the test If PASS is displayed then the analyte concentration is within the measuring range of the selected test If lt FAIL is seen the analyte concentration in the sample is too low and if gt FAIL is displayed the analyte concentration is too high In either of these cases select a different method with an appropriate measuring range or dilute the sample accordingly REFERENCE Q The following section contains charts which detail the programmes available on the four AquaMate methods disks 48 English A
64. e passwords or edit the Current Users Page It is strongly recommended that a new user name and password for the Administrator are set as soon as USER LOG ON is activated and that USER LOG ON is activated whenever the instrument is to be used in a multi user environment After USER LOG ON is enabled each time the instrument is powered up the user will be prompted to log on by entering name and password At the close of a session the user logs off by pressing the LOG OFF function key on the Home Page and choosing whether to PROCEED or STOP When PROCEED is chosen the system waits for the next user to enter their name USER LOG ON can be reset to OFF only by the Administrator After USER LOG ON has been set to OFF the list of users is cleared and the default User Name and Password are both reset to ADMIN HISTORY FILE Toggles between ON and OFF The History File contains the history of the instrument An entry is put into the file when there are changes to EHT calibrations sipper calibrations and CVU tests noting date time and user The file will also contain records of operations carried out by engineers during maintenance visits When HISTORY FILE is set to ON the HISTORY FILE function key becomes available on the Environment Page unless USER LOG ON is enabled and the user has been denied access by the Administrator Pressing this function key brings up the History File Pop up box SAVE HISTORY ON DISK The user is prompted for a file
65. ectangular mg l N2H4 14797 14797P10 FXD 0 005 0 400 mg l 50mm Rectangular mg l N2H4 109711 09711P50 FXD 1 1 00 mg l 20mm Rectangular mg l N2H4 109711 09711P20 FXD Hydrogen Peroxide 2 0 20 0 mg l 16mm Round mg l H202 14731 14731P16 FXD 0 25 5 00 mg l 50mm Rectangular mg l H202 14731 14731P50 FXD 0 05 4 00 mg l 16mm Round mg l Fe 14549 14549P16 FXD 0 03 2 50 mg l 20mm Rectangular mg l Fe 14549 14549P20 FXD 0 02 2 00 mg l 10mm Rectangular mg l N2H4 109711 09711P10 FXD 0 05 5 00 mg l 10mm Rectangular mg l Fe 14549 14549P10 FXD 0 005 1 000 mg l 50mm Rectangular mg l Fe 14761 14761P50 FXD 0 03 2 50 mg l 20mm Rectangular 14761 14761P20 FXD 0 gt 0 0 0 5 1 0 50 0 mg l 16mm Round mg l Fe 14896 14696P16 FXD 1 0 50 1 mg l 20mm Rectangular mg l Fe 14896 14896P20 FXD 0 10 5 00 mg l 16mm Round mg l Pb 14833 14833P16 FXD 0 10 5 00 mg l 20mm Rectangular mg l Pb 14833 14833P20 FXD 0 10 5 00 mg l 10mm Rectangular mg l Pb 14833 14833P10 FXD 5 0 50 0 mgll 16mm Round mg l Mg 14684 14684P16 FXD 5 0 50 0 mg 20mm Rectangular mg l Mg 14684 14684P20 FXD 1 0 50 0 mg l 10mm Rectangular mg l Fe 14896 14896P10 FXD 5 0 50 0 mg l 10mm Rectangular mg l Mg 14684 14684P10 FXD 0 01 2 00 mg l 50mm Rectangular mg l Mn 14770 14770P50 FXD TATTOP20 FXD 14770P40 FXD TASSAPTGFXD 14554P20 FXD 0 05 2 00 mg l
66. ed by labels at bottom of screen Clear Clears entry leaving field or parameter ready for new entry clears pop up and clears error messages Enter Enters changes to field or parameter Run Starts instrument measurement according to current method Home Returns to Home page Zero Base Performs a zero REMOVE THE SAMPLE AND ENSURE THAT THE SAMPLE BEAM IS CLEAR OR CONTAINS THE BLANK SAMPLE RELEVANT TO THE ANALYSIS BEFORE ZEROING THE INSTRUMENT Software The Local Control Software is organised in a tree structure with all functions accessed initially from the HOME Page Movement between the software pages is controlled by function keys or by highlighting with cursor keys and pressing ENTER Home will always go to the HOME page The QUANT and FIXED applications are entirely separate Only one application can be operational at any one time Loading another application will overwrite any current data Local and Computer Control Q From switch on the instrument will automatically be under the HOME control of the LOCAL CONTROL software To enable control from an external computer press REMOTE on the HOME page Control O O O O0 9 will go to the computer providing the instrument is idle REMOTE Q To return control to the Local Control software press the HOME key Control will revert to the Local Control software providing that the instrument is idle and the PC software has relinquished control TEXT ENTRY Page This page is us
67. ed for entry of method operator and sample identities and units in all applications The title displayed at the top of the page depends on which parameter is being edited On entry to this page the name field is filled with the current value AM Issue 3b 07 2001 English 5 GENERAL OPERATOR Enter up to 11 characters for operator ID METHOD ID Enter up to 11 characters for method description UNITS Enter up to 11 characters for units SAMPLE ID Enter up to 11 characters for sample ID Q Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters have been entered press ACCEPT Numbers are entered using the numeric keypad If you make a mistake OjO O ole will act as a backspace or CLEAR will clear the whole entry TEXT ENTRY lololol lt Acts as backspace and clears the last character in the line ACCEPT Enters the names and returns to the calling screen CANCEL Abandons the naming operation and returns the calling page The original entry is not changed SAVE RENAME This page is used for saving or renaming files from any part of the LOCAL CONTROL software The page is used to name a file and or change the ID Q On coming to the page the Filename field is ready for editing Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters have been entered press
68. en ON in all the above modes CELL 1 is assigned as the REFERENCE position and a zero will be performed LAST CELL Sets the number of cells to be used in the range 1 to 7 CELL CYCLES Sets the number of cycles up to 300 For example if set to 4 then each cell will be measured in turn according to the application method being used four times MODE must be set to AUTO SPEED Brings up a pop up list to enable HIGH MEDIUM or LOW rotation speed to be selected AM Issue 3b 07 2001 English 61 CELL PROGRAMMER FUNCTION KEYS INITIALISE Resets the accessory and places cell 1 in the sample beam CELL PROGRAMMER REMOVAL AND REFIT This procedure is essential for the fitting of any of the alternative cell holders available for long pathlength cells single cell thermostatting or the MiniSipper If the Cell Programmer basket is removed replaced at any time the Refit procedure must always be repeated REMOVAL O Holding the basket firmly with one hand unscrew the central screw anti clockwise until the basket is released MD REPLACE THE COVER ON THE OPTOSENSOR and secure the cell holder REFIT O Remove the cover from the optosensor and remove the cell holder A Indentify the position of the keyway on the motor shaft O View the underside of the basket to locate the position of the moulded key A Re locate the basket on the shaft ensuring a positive location of the key in the keyway and tight
69. ened the central screw clockwise C From the CELL PROG page use the INITIALISE function key to correctly align the basket with the spectrophotometer This last action is important to ensure the correct operation of the system Whilst there is significant resistance to manual movement of the motor it is suggested that as routine initialisation of the Cell Programmer should be performed as a check before any measurements are performed 62 English AM Issue 3b 07 2001 SUPERSIPPER SUPERSIPPER Q The SuperSipper is an optional accessory that enables samples to be drawn into a flowcell of the user s choice for automatic measurement After the measurement is complete the sample may be sent to waste or returned to its original vessel A continuous pumping mode allows the system to be washed through when required e g between applications O This section describes the operation of the SuperSipper with the Local Control software Full details of the installation and operation of the accessory are described in the SuperSipper Installation and Maintenance Manual 9499 230 29611 supplied with the SuperSipper Q To operate the SuperSipper install as described in the above manual Present the sample to the SuperSipper and press the switch The required sample volume will be drawn into the tubing When the system beeps remove the sample and the required air gap will be drawn in Once the measurement has taken place press the switch again T
70. ength and performs an autozero BASELINE This re measures the default baseline Ensure that both lamps are on and that the spectrophotometer is fully warmed up This process will take about one hour The default baseline should be re measured whenever one of the source lamps is changed or if the instrument is working at temperatures significantly different from 25 C or if the wavelength calibration is altered INITIALISE WITH D2 Not available in AquaMate Vis This sets the instrument to initialise with or without the Deuterium lamp on If set to ON then the instrument will automatically strike the Deuterium lamp during initialisation WHITE LIGHT Page Q The WHITE LIGHT feature is used to facilitate alignment of optical accessories in the sample compartment Q When the INITIALISE function key is pressed the instrument will align the grating so that the zero order diffraction passes through the sample compartment This provides a beam of white light which can be seen when a white card or similar is placed in the light path Q When alignment is completed pressing the STOP function key returns the grating to its normal position and pressing the SETUP PAGE function key returns to the Setup Page SET UP CVC Page This page allows the CVC calibration data provided on disk to be loaded into the spectrophotometer memory For full details please see the CVC section of this manual 38 English AM Issue 3b 07 2001 SETUP RECO
71. es to the QUANT RESULTS page if there are any results in the table QUANT PAGE Switches back to the Quant page EDIT STD Available after calibration Allows each standard to be used ignored or remeasured EDIT CURVE Available after calibration Allows curve fit to be changed QUANT CALIBRATION Q Press ZERO BASE to zero the instrument with the current method See page 5 Q To calibrate the system press return to the QUANT page and press CALIBRATE The QUANT CALIBRATION graph will be displayed and the instrument will prompt for each standard and replicate in turn As the measurements of the standards proceed the datapoints are marked on the graph When all the standards have been measured the system calculates the equation rescales the graph then draws and displays the line of best fit on the graph Q A calibration can be stopped by pressing the STOP function key The calibration will be aborted and the software will return to the QUANT STANDARDS page Any values obtained will be lost Q Ifa calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and switches to the Quant Results screen AM Issue 3b 07 2001 English 11 QUANT 0 0000 50 000 COEFFICIENT 0 9999 VIEW RESULTS Switches to the Quant Results page if there are results QUANT PAGE Switches back to the Quant page STANDARDS Switc
72. for the wavelength to be used for the first sample Data entry is as for MULTI above When the required wavelengths have been entered press ACCEPT to accept the new list or press CANCEL to return to the FIXED METHOD page leaving the original list unchanged If the wavelength requires the Deuterium lamp if fitted then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH This is fixed at 2 0 nm INTEGRATION Enter integration time in seconds Q This sets the integration time for which the result is measured The current data will be lost if the integration time is changed TIMER S Displays the AquaMateTimers Page Up to four countdown timers may be defined Use the arrow keys to move around the list of timers and press ENTER to change an individual item For each timer select the title to be displayed the duration of the timer and the required action on timeout TITLE Select from TIMER WAIT SHAKE INVERT SWIRL BOIL HEAT DURATION Set a time in the range 00 01 to 99 59 using to separate minutes and seconds ACTION Select from PAUSE CONTINUE If the timeout action is set to PAUSE the instrument will display a prompt when the timer has finished The user is given the choice between stopping zeroing and continuing the measurement sequence If CONTINUE is selected the instrument will automatically continue to the next stage in the measurement sequence Use the
73. glish AM Issue 3b 07 2001 SETUP LIMS SUPPORT Toggles between OFF and ON Q When ON results methods and sample IDs when selected are exported automatically after each measurement to the central LIMS computer via the RS232 port THIS INTERFACE MUST BE CONNECTED BEFORE LIMS SUPPORT IS ACTIVATED USE SAMPLE IDS Choose between OFF SEEDED and PROMPT USER a OFF The system does not attach an identity to the sample a SEEDED Enables the system to be set up to attach an identity to each sample automatically This appears on the screen and the print out It is also exported to the LIMS when enabled with the results of the run and method used Selection of SEEDED causes two additional items to appear in the Environment Menu SAMPLE ID A Enter the name of the sample via the Text Entry Page Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters up to 11 have been entered press ACCEPT If you make a mistake olololoj will act as a backspace or CLEAR will clear the whole entry SAMPLE ID SEED Sets the number to be used for the first sample via the numeric keypad The Sample ID is incremented automatically before each run Set the seed to zero for the results of the first run to be numbered 1 Q PROMPT USER Before each run the Text Entry Page appears and the user is prompted to enter an identity for the sample NOTE a When the Cell Programmer is used i
74. he first timer press the RUN key The screen will show the action to be carried out and the time remaining i e SHAKE REMAINING TIME 02 46 2 The instrument will beep to indicate the end of the time period and a TIMER FINISHED menu box will appear with the following options PROCEED ZERO STOP 3 In order to start the next timer select PROCEED using the arrow keys and press ENTER The next timer will begin At the end of this timer the same TIMER FINISHED menu box will appear 4 Ifthe method procedure requires that a zero measurement be taken insert the blank select the ZERO option using the arrow keys and press ENTER A zero measurement will be taken and a menu box will appear with options to PROCEED or STOP 5 To take a measurement insert the sample into the cell holder and select PROCEED A sample measurement will be carried out and the FIXED RESULTS page is displayed From this page additional samples can be measured by inserting a sample and pressing the RUN key By selecting the STOP option from any of the menu boxes the procedure will be aborted Some procedures require that a zero measurement is taken before the timer sequence is activated In this case perform a zero measurement by inserting the blank and pressing the ZERO BASE key The RUN key can then be pressed to activate the first timer No Q In QUANT mode measurements are automatically taken from the calibration graph in concentration units The uvcalc equation i
75. he sample will be pumped out of the flowcell either to waste or returned to the sample vessel When the sipper is connected a status box is displayed on the right hand side of all the method screens that indicates the presence of the SuperSipper and its status SIPPER Page l SIPPER I Q This page allows the SuperSipper to be set i I up for the required analysis The method set I SIPPER OFF I MODE SIP on this page will be saved with any data AIR GAP 1 50 cm i produced by the software I SAMPLE VOL 1 000 ml I SAMPLE C WASTE Q Toreach this page press ACCESSORIES LOW VOL OFF from the HOME page then select SIPPER i tigre Sate Sap Neal Eaa a Sai RIA TISSUE RIETI and press ENTER To change a parameter highlight the required value using the Up Down Arrow keys and press ENTER SIPPER Selects from ON OFF or STANDBY In STANDBY mode the sipper pumps a small volume approximately every 30 minutes This is to change the point at which pressure is applied to the Sipper tubing thus preventing the formation of a permanent kink The first 6 movements are in the Return direction and the next 6 are in the Waste direction The total volume pumped is sufficiently small as to ensure that any sample present remains in the tubing When the sipper is operated normally its clock is reset and the Standby process re starts MODE Selects from SIP SIP amp RUN CONTINUOUS AUTOSAM AUTOMATIC SIP Sets the system to f
76. hes to the Quant Standards page PRINT GRAPH Prints the Quant method and calibration graph SAVE METHOD Switches to the SAVE page QUANT RESULTS Page a To view further pages of results use the Up and Down arrow keys Q Pressing RUN takes another sample measurement and displays the result Q Results are numbered sequentially and any batch can be of up to 600 samples QUANT RESULTS ABS CONC mg l py 0 201 10 122 2 0 201 10 122 CLEAR RESULTS Deletes all results from the results table QUANT PAGE Switches back to the Quant page SAVE DATA Brings up the SAVE page PRINT LIST Gives a printout of the results LIMS Sends the results via the RS232 port 12 English AM Issue 3b 07 2001 FIXED FIXED Q Instrument and analysis parameters are set up on the FIXED page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q Once the method has been set up press ZERO BASE to zero the instrument for the current method See page 5 and press RUN The spectrophotometer will perform a measurement and display the result on the FIXED RESULTS page Q Once all results have been collected save the data FIXED METHOD Page FIXED l MODE ABS ID l X SELECT SINGLE WAVELENGTH S 550 0 nm BANDWIDTH 2 0 nm INTEGRATION 1s TIMER S 0 LAMP CHANGE 325 nm 1 OPERATOR
77. hod Changing the operator name will not cause any current data to be lost N B If user log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer reference mode if fitted FIXED Method Page Function Keys FIXED OIIO EN VIEW RESULTS Switches to the FIXED RESULTS page SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk PRINT METHOD Prints the current method parameters on the printer Q Pressing RUN starts a fixed measurement using the current method and switches to the FIXED RESULTS page Q Pressing ZERO starts a zero using the current method Any changes to the WAVELENGTH BANDWIDTH INTEGRATION or LAMP CHANGE parameters will invalidate the current results If AUTOPRINTING is selected see SETUP for details a change to the MODE parameter will invalidate the current results AM Issue 3b 07 2001 English 15 FIXED FIXED RESULTS Page Q The layout of the page depends on the Mode and Select in use SINGLE 7 In ABS or T modes up to 2 columns of results are displayed per page Results accumulate on the same page until it is full MULTI Two columns of results are displayed per page Results of each sample always start on a new page SERIAL 7 One column of results is displayed per page Results accumul
78. ich will be remeasured with each press of RUN Up to 9 different QUANT measurements may be specified These measurements may be either a standard S1 S6 measured as part of the normal calibration process or a sample X F Numerical factor to be input by the user at run time Fixed factors are input once before the first measurement of a sequence The value of a Fixed factor will be cleared if you select CLEAR RESULTS from the results page or if you return to the main menu Variable factors are input before every sample measurement R A uvcalc result from a preceeding equation in the list The required formula can then be built up by highlighting the required symbol and pressing ENTER NB Navigation around the screen is achieved in all cases as per the normal operation of the software ie using the arrow keys to move around Q To clear the formula press C To accept it press ACCEPT TITLE amp UNITS allow the user to enter a suitable description and units for the equation AM Issue 3b 07 2001 English 25 UVCALC TEST RESULTS toggles between ON amp OFF whilst the LIMIT lines set the allowable limits for the test CONC VALUES QUANT only toggles between YES and NO If YES is selected the concentrations of standards or samples are used for the Measurement items in the equation Otherwise the measured absorbance values will be used in the calculations HOW TO
79. ill the flowcell If SAMPLE is set to RETURN then alternate switch presses will fill and empty the flowcell In this mode instrument operation is completely independent of the SuperSipper SIP amp RUN Sets the system to fill the flowcell and automatically perform a measurement If SAMPLE is set to RETURN then alternate switch presses will fill and empty the flowcell The current method used to produce the result e g if FIXED is current then the sample will be scanned using the FIXED METHOD as set AM Issue 3b 07 2001 English 63 SUPERSIPPER CONTINUOUS Sets the system to pump continuously to waste Alternate switch AUTOSAM AUTOMATIC AIR GAP presses will start and stop pumping In this mode instrument operation is completely independent of the SuperSipper Sets the system to work with the Gilson 221XL and 222XL Autosamplers Refer to the Autosampler Interface manual for further details In this mode the sipper pump is controlled by the instrument The flowcell is automatically filled when the user presses RUN The sample is always pumped to WASTE in this mode In addition the sipper switch is used to pump continuously to WASTE Alternate switch presses will start and stop pumping Enter value between 0 and 500 cm Sets the gap between the trailing meniscus of the current sample and the leading meniscus of the next sample The gap is measured to the nearest centimetre For best results set the airgap no less than 8cm
80. menu bar Select TRANSFER then CAPTURE TEXT then key STOP to terminate the transfer and save the file Helios HyperT erminal Capture Text gt i Stop 82 English AM Issue 3b 07 2001
81. n AUTO mode the Sample ID is incremented automatically without stopping for ID confirmation between samples b PROMPT USER is not compatible with the Sipper used in Sip and Run or AutoSampler modes DEFAULT FILE TYPE Selects the default file type on the SAVE RENAME page Q Available formats are NORMAL The native file type used by the Local Control software CSV Comma separated variable Use up down arrow keys to highlight choice and press ENTER to confirm selection AM Issue 3b 07 2001 English 35 AUTOSAVE RESULTS SETUP Toggles between ON and OFF When ON results are saved automatically after each run Selecting ON causes two additional items to appear in the Environment menu FILENAME FILE NUMBER Enter a filename of up to 5 characters via the Text Entry Page Enter a number between 0 and 999 via the numeric keypad The number is appended to the filename and incremented automatically after each run AUTOPRINT RESULTS Toggles between ON and OFF When ON results are printed automatically after each run Before attempting to print at any point during operation of the instrument ensure that the printer is ready to print ie switched on connected to the instrument and supplied with paper Failure to do so will result in an error condition Press Clear to clear the error message the system may take a little time to respond Ensure the printer is ready and retry USER LOG ON The default setting i
82. o light can be seen from the ventilation slots in the lamphouse cover The tungsten lamp has failed fit a new lamp A small number of tungsten lamps do fail very rapidly If this happens fit a new lamp and contact your agent for a free replacement It is likely that the spare lamp shipped with the instrument will be from the same batch as the one in the instrument Lamp energy low Error 1053 Drift at visible wavelengths Instrument noisy in the visible region Check the tungsten lamp is correctly fitted and in good condition The envelope should not show any signs of blackening or opacity If in doubt replace with a new one A small number of tungsten lamps do rapidly degrade If this happens fit a new lamp and contact your agent for a free replacement Lamp energy low Error 1053 Noisy signal Cell programmer partially blocking the beam If the carousel is fitted after using another cell holder go to the Cell Programmer page and key Initialise 78 English AM Issue 3b 07 2001 FAULT FINDING 2 Make sure the carousel is not jamming or being fouled on tubing 3 Make sure there are no cells in the beam Fails to initialise Error 3093 1 Check that the sample compartment lid is correctly shut 2 Check that the accessory panel found at the left hand side of the sample compartment is in place 3 Position the instrument so that it is not directl
83. om this page additional samples can be measured by inserting a sample and pressing the RUN key 6 By selecting the STOP option from any of the menu boxes the procedure will be aborted 7 Some procedures require that a zero measurement is taken before the timer sequence is activated In this case perform a zero measurement by inserting the blank and pressing the ZERO BASE key The RUN key can then be pressed to activate the first timer Q Inall cases the relationship between absorbance and concentration takes the general form C A x FACTOR O Therefore the uvcalc equation typically takes the following form Method H1310 FXD Bromine Equation M1 2 25 Q The factors entered are generic We recommend that in all cases the factors are checked with standard solutions appropriate to the laboratory and the equation modified accordingly QNT Files Q The QNT files have the following format Hxxxx QNT where xxxx Hach Program Number QNT QUANT application in software O QNT files are set up for methods that require calibration graphs for each new batch of reagent Q Once the relevant QNT file has been loaded the QUANT page is displayed The file name appears at the top of the screen Operation Q Calibrations have been prepared for most of the Hach QUANT methods These methods are ready for use as soon as they have been loaded However these calibrations may be affected by local conditions We recommend that in all cases user calibra
84. pectronic approved Customer Support Organisation English AM Issue 3b 07 2001 MAINTENANCE ROUTINE MAINTENANCE Very little maintenance is required to keep the spectrophotometer in good working condition The interior should be kept as dust free as possible and the sample compartment cleaned regularly wipe off spilt chemicals immediately Replacement sample compartment liners are available under the following part number Helios Single Beam 9423 UV9 7000E CLEANING INSTRUMENT EXTERIOR The exterior of the instrument can be cleaned periodically as follows CAUTION Do not allow moisture to leak into the instrument Q Switch off the spectrophotometer and disconnect from the mains supply Q Using a lint free cloth dampened with a weak solution of detergent and water wipe the exterior surface of the instrument as necessary Q Wipe over with a cloth dampened with plain water Q Dry the surface with another cloth REMOVAL AND REPLACEMENT OF TUNGSTEN HALOGEN LAMP WARNING Switch off and disconnect the spectrophotometer from the mains and allow the lamp to cool for at least 15 minutes before proceeding a Remove the back corner cover by turning the fastener one quarter turn anti clockwise and slide the cover up to remove a Now remove vertically upwards the metal lamp cover P d Protective Sleeve Silica Envelope Lamp Base Spring Clip O If fitted remove the spring clip AM Issue 3b 07 2001 English
85. ral keypad and LCD display Q The system is composed of a spectrophotometer with integral keypad LCD display 1 44 Mbyte Disk Drive Local Control Software and output device LCD display contrast can be controlled during initialisation or from the HOME page using the left and right arrow keys Q Always remove disks from the disk drive when not in use Never power up the instrument with a disk in place as permanent damage may be caused to the disk The only exception to this rule is when upgrading the instrument software Then automatic recognition of a software disk causes an automatic upgrade of the software Q The Local Control software controls all aspects of the systems operation Q The Local Control software provides automatic calculation of results from measurements using user defined equations in QUANT and FIXED modes User Interface ifj LCD DISPLAY HOME KEY Dea ZERO BASE KEY Ke run KEY OO OO De mcr b C O TI O ARROW KEYS C 1O _ 00 r 89 O NUMERICAL KEYS ENTER KEY CLEAR KEY 4 English AM Issue 3b 07 2001 GENERAL Key Operation Arrow Keys Highlight menu options or move track cursor or move Cell Programmer depending on page in use Change display contrast with lt gt from Home Page Numerical Keys Enter numbers minus and decimal point Function Keys Access and perform system functions Operation depends on screen in use and is indicat
86. rnal printer at any point during operation of the instrument ensure that the printer is ready to print Failure to do so will result in an error condition Press CLEAR to clear the error message Then rectify the problem with the printer and try again ENVIRONMENT Page This page is used to select the language used for the software the use of the beep date format and to enable disable Automatic Calibration Validation and LIMS Laboratory Information Management System Support and to select the default filetype used when saving results i i LANGUAGE ENGLISH SOUND OFF DATE FORMAT dd MM yy AUTOMATIC CAL VAL OFF DEFAULT FILE TYPE NORMAL LIMS SUPPORT OFF USE SAMPLE IDS OFF i AUTOSAVE RESULTS OFF AUTOPRINT RESULTS OFF USER LOG ON OFF HISTORY FILE OFF AUTOMATIC CAL VAL LANGUAGE Select from the list The language used immediately changes to the one selected SOUND Turns the warning beeper ON or OFF If set to OFF then the only indication of any error is the screen message DATE FORMAT Defaults to dd MM yy but will toggle with ENTER to MM dd yy Toggles between OFF and ON with Enter Q When ON and the CVC Calibration Validation Carousel is fitted the instrument automatically waits on start up for the warm up period 60 minutes and then performs the Wavelength and Absorbance calibration tests See CVC Section Pressing Clear aborts 34 the calibration En
87. rom a test up to that point are discarded AM Issue 3b 07 2001 English 71 CVC CVC REMOVAL AND REFIT REMOVAL a Holding the basket firmly with one hand unscrew the central screw anti clockwise until the carousel is released a Replace the CVC as soon as convenient in its protective storage box Q REPLACE THE COVER ON THE OPTOSENSOR unless the Cell Programmer is to be fitted REFIT Q Remove the cover from the optosensor Q Identify the position of the keyway on the motor shaft Q View the underside of the basket to locate the position of the moulded key a Re locate the basket on the shaft ensuring a positive location of the key in the keyway and tightened the central screw clockwise Q From the CVC page use the INITIALISE function key to correctly identify the carousel with the spectrophotometer This last action is important to ensure the correct operation of the system Whilst the spectrophotometer will check for data to carousel match on running any test an initial confirmation is recommended 72 English AM Issue 3b 07 2001 INTERNAL PRINTER INTERNAL PRINTER Q The Internal Printer is a factory fitted thermal head printer It is supplied with a roll of 11 2cm wide single ply thermal paper Paper rolls may be ordered separately under the following part number 4401 161 00391 Q Forlong term storage it is recommended that the paper is kept away from light and at room temperature
88. rum will be lost if any of the method parameters are changed I SCAN MODE ABS I I I I ID TEST 1 START 400 0 nm STOP 600 0 nm PEAK TABLE OFF I GRAPH HIGH 2 000 GRAPH LOW 0 000 I I CELL PROG AUTO I REF MODE _____________i_OFF_________ A MODE Select from ABS T ABS Selects Absorbance T Selects transmittance I Selects Intensity mode This will measure the intensity of the signal in the sample beam The current spectrum will be lost if the Scan Mode is changed ID Enter a description using the TEXT ENTRY screen START Select a wavelength between 190 0 nm 315 0 nm for AquaMate Vis and 1096 0 nm Q Selects start wavelength The Deuterium lamp will be switched on if a wavelength less than 325 0 nm is selected for an AquaMate Vis The Start wavelength must be at least 4 nm less than Stop wavelength The current spectrum will be lost if the Start wavelength is changed AM Issue 3b 07 2001 English 17 SCAN STOP Select a wavelength between 194 0 nm 319 0 nm for AquaMate Vis and 1100 0 nm Q Selects stop wavelength The Stop wavelength must be at least 4 nm greater than the Start wavelength The current spectrum will be lost if the Stop wavelength is changed PEAK TABLE Select from OFF PEAKS TRACK RATIO CORR RATIO PEAK HEIGHT This selects the type of peak picking done automatically as part of the method Results are reported on the Peaks Page
89. s OFF Changing the setting to ON is password protected When set to OFF any user has full access to all the functions of the instrument When set to ON users must identify themselves by user name and password at log on and then have access to whichever functions are enabled for them by the System Administrator Setting User Log on to ON is password protected Use the up and down arrow keys to move the highlight to USER LOG ON and press Enter This brings up the Text Entry Page When the correct password is entered USER LOG ON is set to ON otherwise an error message is displayed and it remains set to OFF The default password is ADMIN Note that the password is case sensitive and in this password all the letters are upper case Logging on as ADMIN gives access to the system at Administrator level and the CHANGE USERS function key is enabled E EDIT METHODS C CALIBRATIONS F DELETE FILES I INITIALISATIONS CURRENT USERS PASSWORD H HISTORY FILE L RESET LIFETIMES CANCEL USERS 11 20 ACCEPT Pressing the CHANGE USERS function key brings up the CURRENT USERS page Up to 20 users can be listed by name and password The privileges of each user can be set individually by the Administrator and can be any combination of Edit Methods Calibrations Delete Files Initialisations History File Reset Lifetimes 36 English AM Issue 3b 07 2001 SETUP Only the Administrator is able to chang
90. s and press ENTER to change an individual item For each timer select the title to be displayed the duration of the timer and the required action on timeout TITLE Select from TIMER WAIT SHAKE INVERT SWIRL BOIL HEAT DURATION Set a time in the range 00 01 to 99 59 using to separate minutes and seconds ACTION Select from PAUSE CONTINUE If the timeout action is set to PAUSE the instrument will display a prompt when the timer has finished The user is given the choice between stopping zeroing and continuing the measurement sequence If CONTINUE is selected the instrument will automatically continue to the next stage in the measurement sequence Use the CHANGE MODE function key to set the OPERATING MODE as MULTIPLE USE or SINGLE USE In SINGLE USE mode the timers will be run before the first measurement only 8 English AM Issue 3b 07 2001 QUANT When the operating mode is set to MULTIPLE USE all timers will be used before each measurement Use the RUN TIMERS function key to operate the timers without initiating a measurement sequence Once the timers have been set up press the ACCEPT function key to accept the new list or the CANCEL function key to return to the FIXED METHOD page without changing the timers Q When RUN is pressed to start a measurement the specified sequence of timers will be started with the remaining time for the current timer being displayed on the screen Any timer may be interrup
91. s therefore of the form Method H1260 QNT Boron Equation M1 In effect the uvcalc equation is used to indicate the chemical form and set the measuring range limits Disk 3 Dr Lange Cuvette and Pipette Test Kit Methods Q All Dr Lange cuvette and pipette test methods are FXD files These files have the following format Kxxxyyy FXD Wxxxyyy FXD where Kxxx or Wxxx last four digits of the Lange test kit FXD FIXED application in software Q Once the relevant method has been loaded the FIXED page is displayed The file name appears at the top of the screen Operation 1 The sample preparation should be carried out according to the instructions supplied with the test kit 2 When the blank has been prepared place it into the cell holder and press the ZERO BASE key The instrument will then zero against the blank 3 Insert the prepared sample into the cell holder and press the RUN key A measurement will be carried out and the FIXED RESULTS page will be displayed 4 From the FIXED RESULTS page additional samples can be measured by inserting the sample and pressing the RUN key 46 English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS Q Inall methods the relationship between absorbance and concentration is linear and takes the general form C A x FACTOR Q Therefore the uvcalc equation typically takes the following form Method K307CT FXD Boron Equation M1 1 74 Q The factors entered are those documented by Dr Lange
92. stem to fill the flowcell and automatically perform a measurement The current method is used to produce the result e g if FIXED is current then the sample will be measured using the FIXED METHOD as set CONTINUOUS Sets the system to pump continuously to waste Alternate switch presses will start and stop pumping The instrument will not process any key presses while the MiniSipper is pumping in continuous mode AUTOMATIC In this mode the sipper pump is controlled by the instrument The flowcell is automatically filled when the user presses RUN The sample is always pumped to WASTE in this mode In addition the sipper Switch is used to pump continuously to WASTE Alternate switch presses will start and stop pumping 66 English AM Issue 3b 07 2001 MINISIPPER AIR GAP Enter value between 0 and 500 cm Sets the gap between the trailing meniscus of the current sample and the leading meniscus of the next sample The gap is measured to the nearest centimetre For best results set the airgap no less than 8cm from the flowcell SAMPLE VOL Enter a value between 0 5 and 9 999 ml Sets the volume of sample to be pumped SAMPLE Set to WASTE After measurement the sample is pumped through the flowcell to waste by the act of pumping the next sample LOW VOL Set to OFF A standard internal diameter 4 0mm uptake tube is used FUNCTION KEYS VIEW CALIB Goes to the SIPPER CALIBRATION page CALIBRATE Starts the Sipper calibra
93. t FIXED and press ENTER O Enter a suitable ID for the factor and press ACCEPT A Highlight and press ENTER A Highlight F and press ENTER This time select Variable Factor from the pop up Q Enter a suitable ID for the factor and press ACCEPT Q Highlight and press ENTER Q Press ACCEPT to return to the main uvcalc page a Press ACCEPT twice more to return to the FIXED page Q Insert sample and press RUN You will be prompted when to input the factor values AM Issue 3b 07 2001 English 27 UVCALC How to modify an existing FIXED uvcalc equation e g to modify the initial equation M1 50 0 so the equation becomes M2 M1 50 0 Where M1 becomes a once only constant and M2 is measured with each run a On the FIXED page select UVCALC and press ENTER Q Select EQUATION 1 and press ENTER The equation should now be displayed Q Select FORMULA and press ENTER The cursor should be at the end of the existing formula Q Press SWITCH FIELDS and navigate the cursor to M1 Press ENTER Q Alter selection to ONCE ONLY Q Press SWITCH FIELDS Q Enter the M2 and For M2 select MEASURE WITH EACH RUN Q Press SWITCH FIELDS and move the cursor to the Q Press SWITCH FIELDS again and add the closing bracket Q Press ACCEPT This will return you to the main uvcalc page O Make appropriate entries for the TITLE and UNITS line Press ACCEPT twice O This will return yo
94. ted by selecting the STOP softkey Once the last timer has finished the measurement will be made in the normal way N B Timers can be used in conjunction with a sipper in AUTOMATIC mode They cannot be used with a cell programmer in AUTO mode LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Not available in AquaMate Vis Q Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 or W overrides any changeover and the selected lamp will be used regardless of the wavelength set Any current data will be lost if the lamp changeover wavelength is changed OPERATOR Switches to the TEXT ENTRY screen The operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost If User Log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen Q See UVCalc Section CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer Reference Mode if fitted AM Issue 3b 07 2001 English 9 QUANT QUANT Page function keys QUANT VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration exists VIEW RESULTS Switches to the Quant Results page if there are any sample results SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk
95. th regard to this material including but not limited to the implied warranties of merchantability and fitness for a particular purpose All world rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other record without the prior written permission of Thermo Electron Corporation For technical assistance please contact Technical Support Thermo Electron Corporation 5225 Verona Road Madison WI 53711 4495 U S A Telephone 1 800 642 6538 U S A or 1 608 273 5015 worldwide Fax 1 608 273 5045 worldwide E mail techsupport analyze thermo com ABBE 3L AIR Almega AMINCO Bowman Antaris Analyze Detect Measure Control ApPro AquaMate ARK Atlus ATMOS Aurora Avatar BACKCOMM Baseline BEAM BioMate Blocker Centaurus Chemigram CIRCLE Collector Contact Alert Contact Sampler Continuum Corner Cube CSA 4K DeterminatIR DSolve DX ECO Educator ENCORE Encompass Endurance E S P Ever Glo Evolution EZ Flow EZ Scope Far IR Solid Substrate FiberLink FiberPort FLEXSYS Flex Top Foundation Series Full Field Aperture Gemini Genesis II GENESYS G XT Helios ImageMax Impact Import Infinity Infinity Gold InspectIR Instruments of Discovery Integra Interlinking InterpretIR InterpretIR IR Plan IRus ISOBE Laser Quadrature Lock in Line Macros Basic Macros Pro Magna
96. tion procedure Sipper Calibration Q This calibrates the SuperSipper to take account of variations in pump and uptake tubing and sample viscosities A volume is set and using the appropriate solvent and tubing several sips are performed The actual volume sipped is entered and a calculation done to produce a calibration factor This factor is then used to adjust the pumping time to ensure that the correct volume of sample is always used Q Details of the calibration used are displayed on the SIPPER CALIBRATION page CALIBRATE SIPPER Page ALA ALA Q Using the solvent which will be used for the sample solutions offer a measuring cylinder CALIBRATE SIPPER filled to the highest gradation to the sipper NOMINAL VOL 1 000 ml 25 NO SIPS DONE uptake tube and press the switch The sipper will pump a sample and the spectrophotometer will issue a beep Withdraw the measuring cylinder and the sipper will pump the air gap The values used for sample volume and air gap are those set on the SIPPER page Q Repeat this process for a number of cycles up to a maximum of 10 then press ENTER Q Measure the total volume taken from the measuring cylinder and enter this The calibration will be displayed AM Issue 3b 07 2001 English 67 MINISIPPER FUNCTION KEY SIPPER PAGE Returns to the SIPPER page and abandons the calibration SIPPER CALIBRATION Page OI 1 O This page displays the current sipper i SIPPER CALI
97. tions should be performed with standard solutions appropriate to the laboratory and stored under a new method name Q Ina few cases new calibrations are required for each reagent batch or plating bath formulation These methods must be calibrated by the user before they can be used Q General instructions for performing a calibration follow below Specific instructions and details of standard preparation are included in the PDF file for the method a The standards to be prepared can be viewed by selecting STANDARDS from the QUANT page using the arrow keys and pressing ENTER These standards should be compared to those detailed in the on the Hach procedure sheet and should then be prepared If the preparation of standards requires the same timers as the samples then the timers can be run by selecting the TIMER S option from the QUANT page using the arrow keys and pressing the softkey RUN TIMERS b Once the standards are ready for measurement press the CALIBRATE softkey Follow the on screen instructions to measure the standards When all standards have been measured the calibration graph is shown along with the coefficient AM Issue 3b 07 2001 English 45 AQUAMATE METHODS DISKS c Atthis point press the SAVE METHOD softkey and save the method file either to the library or methods disk The programme is now ready to use for measuring samples 1 The Hach procedure should be followed until the first timer is required In order to start t
98. u to the FIXED page 28 English AM Issue 3b 07 2001 UVCALC ERROR MESSAGES The following error messages may occur if you make a mistake in entering an equation or in setting the system up ONLY 1FACTOR MAY BE ENTERED WITH SAMPLE You have attempted to create a formula with two or more factors for each sample THIS FORMULA HAS TOO MANY CONSTANTS You have created a formula with more than 9 numbers in it FORMULA CONTAINS AN INVALID NUMBER You have attempted to create a formula with an invalid input BRACES DO NOT MATCH IN FORMULA You have created a formula with too many brackets at one end ALL BINARY OPERATIONS REQUIRE TWO OPERANDS You have created a formula with an incomplete arithmetical operation e g 3 4 INVALID COMBINATION OF OPERANDS You have created a formula with missing operator s e g F1 M1 BRACE MISSING UNMATCHED CLOSE BRACE You have created a formula with a close brace bracket before or without an open brace THIS FORMULA CANNOT START WITH THIS TOKEN You have created a formula with an invalid initial token i e an operator rather than an operand FORMULA CONTAINS OUT OF RANGE STANDARD In QUANT mode a specified standard is no longer in the calibration ONLY ONE MULTIPLE MEASUREMENT IS ALLOWED In FIXED mode you have created a formula with an invalid initial token FORMULA CONTAINS INVALID RESULT TOKEN The result from an earlier calculation is no longer being produced UVCALC INVALID CELL PROGRAMM
99. ug l 3500 H3500 FXD Tannin and Lignin 0 9 0 mg l 3550 H3550 QNT Turbidity 0 5000 FAU 3750 H3750 QNT Volatile Acid 0 2800 mg l 3800 H3800 QNT 0 3 000 mg l H3850 QNT 56 English AM Issue 3b 07 2001 AQUAMATE METHOD DISKS Lange Disk 3 anaye Range Units con Programno AquaMate File no BOD Oxygen demand 0 5 12 mg l BOD5 11 mm round LCK554 K554CT FXD biological 5 day Carbonate Carbon dioxide 55 550 mg l LCK 388 K388CT FXD Chloride 70 1000 mg l LCK 311 K311CT FXD Chlorine Total 0 05 1 5 mg l mg l Cla 11mm round LCW 510 W510RC FXD 0 05 1 5 mg l mg l O3 0 03 0 4 mg l mg l Cla 50mm Rectangular LCW 510 W510P50 FXD 0 03 0 4 mg l mg l O3 Chromium 0 03 1 0 mg l mg l Cr 11mm round LCK 313 K313CT FXD 0 005 0 25 mg l mg l Cr 50mm Rectangular LCK 313 K313P50 FXD COD Oxygen Demand Chemical Copper Cyanide Detergents Anionic Tomm Rectangular Formaldehyde 0 01 1 0 mg l mg l HCHO 50mm Rectangular LCK 325 K325P50 FXD 0 5 10 0 mg l mg l HCHO 11mm round LCK 325 K325CT FXD Hydrazine 0 01 2 0 mg l mg l N2H4 10mm Rectangular LCW 025 W025P10 FXD Iron 0 01 1 0 mg l mg l Fe LCK 521 K521CT FXD 0 2 6 0 mg l mg l Fe LCK 321 K321CT FXD 0 2 6 0 mg l mg l Fe II LCK 320 K320CT FXD Manganese 0 02 1 0 mg l mg l Mn 50mm Rectangular LCW 032 W032P50 FXD 0 2 5 0 mg l mg l Mn 10mm Rectangular LCW 032 W032P10 FXD Nickel 0 05 1 0 mg l mg l Ni 50mm Rectangular LCK 537 K537P50 FX
100. y measurements ALWAYS CHECK THAT THE SAMPLE BEAM IS CLEAR BEFORE MEASURING LAMP ENERGIES Abnormal results will be produced if a sample is left in the beam when ZERO BASE is pressed ALWAYS ENSURE THAT THE SAMPLE IS REMOVED AND THAT THE SAMPLE BEAM IS CLEAR OR CONTAINS THE APPROPRIATE ZERO REFERENCE BEFORE ZEROING THE INSTRUMENT VERY OFTEN POOR INSTRUMENT PERFORMANCE OR FAILURE CAN BE ATTRIBUTED TO SIMPLE FAILURE OF THE TUNGSTEN LAMP THEREFORE REPLACE AS BELOW USING THE SPARE LAMP PROVIDED BEFORE SEEKING FURTHER ASSISTANCE If any fault occurs including the above lamp failure these are reported by the system as an Error condition and an Exxxx number is generated Descriptive text is also included with this message DETAILED BELOW ARE THE ERROR CODES PRODUCED IF 1 The tungsten lamp fails or is poorly aligned E3010 E3011 E3104 E3015 E3030 2 The deuterium lamp fails not in AquaMate Vis E3003 E3004 E3005 E3006 E3007 E3008 E3009 E3012 E3013 E3022 E3029 E3044 E3045 3 The beam is blocked on initialisation E3027 E3056 4 The cell programmer is stalled in use or fails to initialise E3001 E3002 E3054 E3055 E3082 E3084 5 The sample compartment is open E3053 E3062 E3068 E3069 E3071 A comprehensive list of these codes is available in the Service Manual for this product Generation of a code not related to replacement of either the tungsten or deuterium lamps usually requires you to contact your local Thermo S
101. y in strong sunlight 4 If using a water circulator ensure that black water tubing is used Deuterium lamp Lamp energy is 1 Lamp energy was measured with a cell in energy low indicated as low but the beam Remove all cells and re performance seems measure the lamp energy OK Deuterium Lamp Performance is poor 1 Plastic cells that do not transmit in the UV energy low in the UV region are being used 2 The deuterium lamp may need replacing Fails to initialise Error 3027 1 Check that the beams are clear and retry 2 Record all the error messages 3027 is a general failure message it is usually preceded by more specific error codes 3 Check that the tungsten lamp is working 4 Check that the cell programmer is not blocking the beam 5 If the problem persists insert a blank formatted disk into the disk drive Switch the instrument on whilst holding down the RUN key Debug data will be sent to the disk Email the file to customer support for further help AM Issue 3b 07 2001 English 79 FAULT FINDING Connecting AQUAMATE to a PC Terminal programmes You will need software that allows you to write to and read from the computer s serial port The communications parameters are Baud rate 9600 Data bits 8 Stop bits 1 No parity Flow control off Local Echo on HYPERTERMINAL You can also use HyperTerminal that comes with Windows 95 To establish a connection start HyperTerminal and select New Connection

AquaMate Operator`s Manual

Contents

Download Pdf Manuals

Related Search

Related Contents