Leica M50 Leica M60 Leica M80 User Manual
Contents
1. ON OFF button The green LED on the upper left corner now lights up User Manual 2 Adjust the brightness by briefly pressing the or buttons 3 Switch off the illuminator by briefly press ing the button Leica LED5000 CXI Coax Illumination H The intensity of the illuminator can be adjusted in 10 increments A The Leica LED3000 NVI can also be controlled via the Leica Application Suite LAS or the Leica SmartTouch H Using the Leica LED5000 CXI results in an increased magnification level of 1 5x Depending on the components used different intensities of vignetting may occur at low magnification Vignetting is normal and not a malfunction Leica M series Use The light of the Leica LED5000 CXI can be very bright Therefore always switch on the illuminator before you look through the eyepieces 1 Switch on the illuminator by briefly press ing the ON OFF button The green LED on the upper left corner now lights up User Manual Adjust the brightness by briefly pressing the or buttons Switch off the illuminator by briefly press ing the button Dimensional Drawings Leica M50 Leica M50 with small incident light base transmitted light base Leica L2 illumination and 45 binocular tube il n 300 321 205 _ S 277 220 R2. Lamp housing Never open the lamp housing while the lamp is switched on Risk of explosion UV exposure blinding Before opening the lamp housing allow it to cool off for at least 15 minutes Danger of explosion Never cover the air duct on the lamp hous ing Danger of fire Leica M series Mercury lamp Follow the user manual and safety regula tions provided by the lamp manufacturer and in particular the section on how to proceed if a lamp breaks and releases mercury For transport remove the mercury lamp transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors When it has reached the end of its rated life follow the manufacturer s specifications and the minute meter on the supply unit To minimize the risk of explosion replace a discolored mercury lamp promptly Leica Microsystems assumes no liability for damage caused by exploding incor rectly installed or improperly used mercury lamps User Manual Table of Contents Inhaltsverzeichnis Leica LED5000 CXI cont d 24 Cenerai instructions 9 Leica LED3000 NVI Near Vertical Illumination 25 Important Safety Notes 3 Leica LED3000 NVI cont d 26 Symbols Used 4 l i Safety Regulations 5 Quick Start Guide Safety Regulations cont d 6 The Reliable Route to Success 28 Safety Reg
3. Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove 5 While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct position Leica M series User Manual Leica LED3000 NVI Near Vertical Illumination Required tools Installing the Leica LED3000 NVI None 1 Connect the Leica LED3000 NVI to the focusing column via the CTL2 connection if the focusing column is equipped with inte The Leica LED 3000 NVI can be used grated electronics with any objectives that have an outer diameter of 58 mm The supported working distance is between 50 mm and 150 mm Leica M series User Manual Alternative installation for manual columns 1 Connect the external power supply unit 10 450 266 to the Leica LED3000 NVI Place the Leica LED3000 NVI on the optics carrier from below and tighten the locking screw 25 Leica LED3000 NVI cont d Ensure that the locking screw of the A illuminator and the locking screw of the optics carrier are on a straight line as otherwise image shading may occur Leica M series User Manual Quick Start Guide The Reliable Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner and naturally you
4. Leica M50 Leica M60 Leica M80 User Manual MICROSYSTEMS General Instructions Safety concept Before using your microscope for the first time please read the Safety concept brochure included with your instrument It contains addi tional information about handling and care Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept i Concepto de seguridad Concept de s curit Concetto di sicurezza Dro bas noteikumi Saugos samprata Biztons gi el r sok Veiligheidsconcept Sikkerhetskonsept Koncepcja bezpiecze stwa Conceito de seguran a Bezpe nostn pokyny Varnostni koncept Turvallisuus S kerhetskoncept ERE AA ee ROSTERS zekat SERE Leica M series Use in clean rooms The Leica M series can be used in clean rooms without any problems Cleaning Do not use any unsuitable cleaning agents chemicals or techniques for cleaning ve Never use chemicals to clean colored surfaces or accessories with rubberized parts This may damage surfaces and abraded particles may contaminate prepa rations In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms User Manual Servicing ve Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Responsibilities of person in
5. Rotate the eyepieces counterclockwise in the direction as far as they will go 5 diopter settings User Manual 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the lowest to the highest level the specimen is always brought into sharp focus If not repeat the process Graticules Use Leica graticules make length measurements and counting easier particularly for work stations that are not equipped with a digital camera and LAS software The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica M series Clamp the graticule on the insert applying 4 You can now align the graticule by rotating moderate pressure Ensure that the grati the eyepiece in the tube and then tighten cule fits tightly ing it using the clamping screw Screw the insert and graticule firmly into place and replace the eyepiece in the tube User Manual 41 ErgoTube 10 to 50 ErgoTube 10 to 50 The ErgoTube with continuously adjustable viewing angle from 10 to 50 allows for opti mum sitting posture Users who work with the same stereomicroscope can adjust the viewing height to their height with a s
6. The focusing drive can be operated either left or right handed The coarse fine adjustment carries a load of up to 15 kg The resolution of the coarse fine adjust ment is 1 um Leica M series User Manual 32 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focus movement too loose or too tight Does the outfit tend to slide downwards The resistance can be adjusted individually depend ing on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing Leica M series User Manual 33 Changing the Magnification Zoom All M series microscopes have an integrated zoom The name indicates the zoom range covered ve Leica M50 5 increments Leica M60 6 1 zoom Leica M80 8 1 zoom The rotary knob for the zoom can be used either left or right handed Leica M series Zooming 1 Look into the eyepieces 2 Focus on the specimen 3 Rotate the magnification changer until the desired magnification is configured User Manual 34 Ratchet Steps and Magnification Levels The zoom button can optionally be operated either with or without ratchet steps Continu ous zoom is possible when the ratchet steps are disabled which many users find convenient On the other hand when the ratchet steps are enabled photographs measurem
7. transmission path for bacterial and viral infec tions of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Safety Regulations cont d Light sources safety regulations Protective measures of the manufacturer UV protection screen in front of the speci men plane prevents the user from looking directly into the UV rays Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes UV filters are installed in the observation beam paths to protect the eyes The stray light protection on the lamp housing prevents irradiation of the hands Leica M series Warning UV radiation could damage the eyes Therefore Never look into the light spot on the speci men plane without a UV protection screen Never look into the eyepieces if no excita tion filter is in the beam path Fill empty filter positions with dummy filter carriers e g M165 FC Do not select a white strongly reflective background for the specimen User Manual Supply unit Always unplug the supply unit from the power supply When installing and disassembling the lamp housing Before opening the lamp housing When replacing the high pressure mercury lamp and other parts such as the heat absorbing filter or the collector During maintenance work on the supply unit Safety Regulations cont d
8. work as well as it did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica M series Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Schweiz AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com User Manual Care Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or replaced unless doing so is specifically permitted and described in this manual Protect your microscope from oil and grease Do not grease guide surfaces or mechani cal parts 64 Care Maintenance Contact Persons cont d Protection from dirt Dust and dirt will affect the quality of your results Puta dust cover over the microscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica M series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuitable cl
9. charge of instrument Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Important Safety Notes User Manual The individual modules of the Leica M stereo microscopy series include an interactive CD ROM with all relevant user manuals in 20 other languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site at www stereomi croscopy com This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains impor tant instructions for their operational safety maintenance and accessories The Safety concept booklet contains additional safety information regarding the service work requirements and the handling of the stereomi croscope accessories and electrical accessories as well as general safety instructions Leica M series You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety regulations To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings containe
10. column using the screw provided Leica M series Alternative installation options Depending on the desired working distance the microscope carrier can be installed in the stan dard way or upside down see figure below User Manual Optics Carrier Tools used None Leica M series Assembling the optics carrier 1 Unscrew the clamping screw on the holder for the optics carrier 2 Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other User Manual 2 Tighten the clamping screw below the Optics carrier Tube All intermediate tubes that fit between the Assembling the tube optics carrier and the binocular tube are fitted 2 Push the tube for example the inclined in the same manner binocular tube into the dovetail ring and rotate it slightly in both directions until the Tools used positioning screw meshes with the guide No tools required groove Preparation 1 Unscrew the positioning screw and remove the protective cover Leica M series User Manual While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct position 20 Eyepieces Tools used Preparation Inserting the eyepieces No tools required 1 If you want to use an optional graticule 3 Push the eyepieces into the tubes as far as insert
11. extension and binocular ErgoTube 0dr xew LIA 067 Ul 600 1E9 61 User Manual Leica M series Appendix Leica M series User Manual 62 Calculating the Total Magnification and Field of View Diameter Parameter Example MO Magnification of objective MO 1x objective ME Magnification of eyepiece ME 25x 9 5 eyepiece Z Magnification changer position Z Zoom position 4 q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 q Coaxial reflected light 1 5x tube factor ErgoTube r Factor 1 25x r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Calculation example magnification in binocular tube Calculation example field of view diameter in the specimen MTOTVIS MO x MExzxqxr or 1x 25x 4x 1 5 x 1 25 187 5x Leica M series User Manual 63 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance microscope Leica microscopes are renowned for their robustness and long service life Observing the following care and clean ing tips will ensure that even after years and decades your Leica microscope will continue to
12. of the Leica M50 M60 and M80 adds three new high quality routine stereomicroscopes to the proven M Series range from Leica Microsystems The optical brilliance and the wide range of acces sories make them the ideal microscope for quality assurance and similar industrial applications Magnification in stages or zoom The Leica M50 stereomicroscope includes precisely reproducible magni fication levels for repeated examinations measurements drawing or photography of objects under absolutely identical conditions The five easily selectable positions can be set without removing the eyes from the eyepieces This ensures that your results remain comparable at all times without great efforts The Leica M60 and M80 stereomicroscopes can be used for a wide range of routine applications with the 6 1 8 1 zoom and switchable ratchet steps The large working distance and the brilliant imaging power shows you the finest details of your specimens without losing your overview of large workpieces Leica M series Common to all microscopes is the Leica range of accessories Whether you need a variety of illumination types a wide selection of objectives or the Leica swinging arm system there is a solution for everything there Do you already own stereomicroscopic equipment and are thinking of switching to Leica No problem Leica M50 M60 and M80 fit into micro scope carrier with a diameter of 76 mm and are therefore compatible with many suppliers Y
13. to photography the digital Leica DFC cameras are exactly right for you Together with the Leica Application Suite they provide virtually limitless freedom of use For additional informa tion about Leica cameras refer to the camera s documentation User Manual Leica Application Suite The Leica Application Suite or LAS for short is as it were the digital extension of the Leica M series microscopes In addition to capturing images it lets you control the microscope illu minator stages cameras and more For addi tional information refer to the LAS online help 44 Photo Tubes and C mounts Application All Leica DFC cameras are equipped with a standardized C mount interface In turn the C mount adapter for the respective trinocular tube is connected to this interface This adapter creates a solid mechanical connection between the microscope and camera and ensures opti mum rendering of the microscopic image on the image sensor of the camera Usually the ideal is for the digital camera to capture as much of the field of vision as possi ble while excluding as much of the black edge of the field of vision as possible To do so the magnification factor of the C mount adapter must match the image format of the sensor as closely as possible see table Leica M series If there is unwanted shading at the corners even with a compatible C mount adapter installed it can be corrected using the Shading functio
14. want to get right to work This manual will familiarize you with the finer details of your micro scope The following pages contain important practical information that makes using it every day easier Take time to read it it s worth it Leica M series User Manual 28 Overview of an M Series Microscope _ Eyepieces for spectacle wearers with dioptric correction and eyecups Fastening screws for the eyepieces Ergo tube Optics carrier Magnification changer zoom Interchangeable objective Incident light base Column io O N DA HF KR W N Cable duct with cover 10 Coarse fine focusing Leica M series User Manual 29 The Correct Interpupillary Distance The interpupillary distance is correctly set if you Adjusting the interpupillary distance see a single circular image field when looking 1 Look into the eyepieces at a specimen 2 Hold the eyepieces with both hands Push If you are still a novice microscope user you the eyepieces together or separate them may need a short time to become accustomed until you see a circular image to this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measures approx 22 mm for 10 23B wide field eyepieces for persons wearing glasses Leica M series VENETE 3 Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting 30 Using t
15. M series Tubes The interface between the optics carrier and the tube has remained the same so existing tubes fit the new M series The new tubes are designed for eyepieces with field number 23 while the predecessor models were only designed for field number 21 resulting in a smaller object field Eyepieces The new M series eyepieces have an audible and tangible click to provide immediate feed back in case of accidental adjustment User Manual On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on Page 29 Leica M series If on the other hand you are assembling the microscope yourself continue with the Assem bly chapter which begins on Page 16 User Manual Assembly Leica M series User Manual 16 Base and Focusing Column The first step is to connect the focusing column Assembling the column adapter 2 Install the base on the column using the of the M series to the corresponding base 1 Remove the three screws provided from the three screws column Tools used Hex socket screwdriver 3 mm Tip Place the column on the edge of a table to attach the base and have another person help you Leica M series User Manual 17 Microscope Carrier Tools Allen screwdriver provided Assembly The microscope carrier is fastened to the
16. d in these user manuals User Manual Symbols Used Warning of a danger This symbol indicates especially impor A tant information that must be read and complied with Failure to comply can cause the following Hazards to personnel Functional disturbances or instruments damaged Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following x Hazards to personnel Functional disturbances or instruments damaged Leica M series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional informa H tion or explanations that are intended to provide clarity Explanatory notes x This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Safety Regulations Description The individual modules fulfill the highest requirements for observation and docu mentation of Leica stereomicroscopes of the M series Designated use Refer to Safety Concept booklet Non intended use Refer to Safety Concept booklet Never use M series microscopes or their compo nents for surgical procedures e g on the eye unless they are speci
17. eaning agents and techniques can damage polymers User Manual Permitted measures Clean the microscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a pneumatic rubber bulb or with a soft brush Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol 65
18. ent results etc can be reproduced more accurately Leica M series Enabling and disabling ratchet steps 1 Push the button towards the knob to enable the ratchet steps 2 Push the button away from the knob to disable the ratchet steps User Manual Magnifications and fields of view The formula on page 62 provide additional information about the magnifications and field of view diameters with consideration given to the position of the magnification changer and the eyepiece and objective combination used Eyepieces amp Tubes Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the micro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The following eyepieces are available for the M series Magnification Dioptric correction Order number 10x 5 diopter settings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 026 Leica M series User Manual 37 Health Notes Potential sources of infection Direct contact with eyepieces is a A potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner Separate eyecups are an effective way of p
19. ferent cameras Separation ratios The HD V allows for selecting three different separation ratios 50 light at all outputs i e 50 light for stereoscopic observation and recording at 50 light 100 light in the right eyepiece for moni toring the object and 100 light in the camera to have the maximum light for the recording under extremely poor light conditions Leica M series Three dimensional observation with 100 light in the binocular tube The HD F features a fixed separation ratio of 50 in the binocular tube and 50 in the photo beam path This allows the user to conduct three dimensional observations of the samples in the binocular tube manipulate and edit them and simultaneously project the image live on the monitor Since the sensors of state of the art cameras are highly sensitive the exposure time for most applications is short in spite of lower light intensity User Manual Videotube HD V Phototube HD F 46 Objectives and Optical Accessories The Different Types of Objectives To meet the various requirements regarding imaging properties there is a choice of high quality interchangeable planachromatic and planapochromatic objectives and also lower priced interchangeable achromatic objectives Achromatic objectives are particularly suited for specimens with high contrast structures x Flat field planachromatic objectives are particularly well suited for studyin
20. fically intended for that purpose The instruments and accessories described in this operating manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Leica M series Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use Refer to Safety Concept booklet Set up the electrical components at least 10 cm from walls and combustible objects Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort measurements and micro graphic images In warm and warm damp climatic zones the individual components require special care in order to prevent the buildup of fungus User Manual Responsibilities of person in charge of instrument Refer to Safety Concept booklet Ensure that The M series stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only All operators have read understood and observe this User Manual and particularly the safety regulations Safety Regulations cont d Repairs service work Refer to Safety Concept booklet Only original Leica Microsystems spare parts may be used Before opening the instruments switch off the po
21. g flat objects such as wafers and thin sections With planapochromatic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Leica M series Achromatic objectives The 0 32x 0 5x 0 63x 0 8x 1x 1 5x 2x achro matic objectives offer countless variants for selecting the object field diameter magnification ranges and working distances see page 63 Planachromatic objective 1x For the highest requirements for overall image quality we recommend equipping the micro scope with the 1x plan flat field objective which returns sharp contrast rich object fields User Manual Achromatic objectives with a long focal length For special applications achromatic objectives with long working distances and focal lengths of f 100 mm to 400 mm are available 48 System Illumination Leica LED3000 NVI Near Vertical Illumination H The intensity of the illuminator can be adjusted in 10 increments H The Leica LED3000 NVI can also be controlled via the Leica Application Suite LAS or the Leica SmartTouch The supported working distance is between 50 mm and 150 mm Leica M series Use The light of the Leica LED3000 NVI can be very bright Therefore always switch on the illuminator before you look through the eyepieces 1 Switch on the illuminator by briefly press ing the
22. he Eyepieces The eyepieces form the connection between If you do not wear glasses If you wear glasses the tube and the eye of the observer Simply 1 Hold the eyepiece firmly and rotate the 1 Hold the eyepiece firmly and rotate the push them into the tube and they are ready to eyecups forwards counterclockwise eyecups counterclockwise towards the rear use as otherwise the viewing distance is too great Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens 2 If an eyepiece is equipped with the inte Dioptric correction grated dioptric correction turn the value to A built in dioptric correction is available for the 0 mark 2 If an eyepiece is equipped with the inte eyeglass wearers For more information refer grated dioptric correction turn the value to to page 39 the 0 mark By the way one benefit of viewing with eyeglasses is a drastically lower risk of bacte rial transmission see page 38 The soft mate rial of the eyecup also ensures that your glasses will not be scratched even if they contact the eyepiece Leica M series User Manual 31 Focusing Focusing raises or lowers the stereomicroscope using the focusing drive The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective
23. i la 390 Leica M series User Manual 53 Leica M50 Leica M50 with large incident light base and ErgoTube 45 514 II pei Ill 500 24 User Manual Leica M series Leica M50 Leica M50 with small swinging arm stand and 45 binocular tube bo 260 fini T ti S 709 220 Leica M series User Manual 55 Leica M60 Leica M60 with large incident light base and 45 binocular tube i i 288 301 User Manual Leica M series Leica M80 Leica M80 with large incident light base and 45 binocular tube Leica M series User Manual 57 Leica M80 Leica M80 with transmitted light base TL ST and binocular ErgoTube Leica M series User Manual 58 Leica M80 Leica M80 with standard swinging arm stand and 45 binocular tube 598 173 IL Leica M series User Manual 59 Leica M80 Leica M80 with Leica LED2500 and binocular ErgoTube Leica M series User Manual 60 Leica M80 Leica M80 with universal stand XL
24. imple hand move ment If the head posture is changed the view ing height can also be readjusted without having to adjust the height of the chair Special long tubes enable the user to maintain an upright posture In order to safeguard against ESD the ErgoTube has been constructed from anti static materials Leica M series Assembly Assembly of the ErgoTube 10 to 50 is exactly the same as that of any other tube see Page 20 Handling 1 Then grasp the tubes with both hands and adjust the desired tilt User Manual Photography amp Video Photography amp Video For most microscope users digital documen tation has become an invaluable part of their work Research results can be presented in an attractive manner measurements on the digi tal image provide clarity and in conjunction with the motorized IsoPro cross stage even images of large specimens can be captured step by step and automatically joined to create a new complete image Adapter If camera control using the Leica Application Suite is not required conventional mirror reflex and rangefinder cameras from third party manufacturers can be used For this purpose Leica Microsystems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica M series Leica DFC cameras However if you require absolute control over the camera and need the capability for measurement evaluation and more in addition
25. it now Page 43 they will go and check to ensure that they Magnification range fit tightly and accurately You can extend the overall magnification range 2 Removethe plastic tube guard using available 10x 16x 25x and 40x wide field eyepieces for persons wearing glasses 4 Securely tighten the clamping screws Leica M series User Manual 21 Objective Tools used No tools required Preparation 1 Remove the protective cap on the optics carrier by turning it Leica M series Attaching the objective Hold the objective firmly during assem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove all specimens from the stage plate first 2 Screw the objective clockwise into the optics carrier User Manual 22 Leica LED5000 CXI Coax Illumination Required tools Installing the Leica LED5000 CXI 2 Unscrewthe positioning screw and remove None 1 Connect the Leica LED5000 CXI to the the protective cover focusing column via the CTL2 connection if the focusing column is equipped with inte grated electronics Alternative installation for manual columns 1 Connect the external power supply unit 10 450 266 to the Leica LED5000 CXI Leica M series User Manual 23 Leica LED5000 CXI cont d 3 Place the Leica LED5000 CXI on the optics 4 carrier and tighten the positioning screw
26. n of the camera software Alternatively you can also use a C mount adapter with higher magnification This primar ily avoids the critical border area of the field of vision and concentrates on the center of the field of vision Camera optimal suitable large smaller image field image field DFC295 DFC290 HD 0 5x 0 63x DFC425 DFC425 C 0 5x 0 63X DFC495 0 63x 0 8x DFC500 0 63x 0 8x User Manual Cameras from third party suppliers In addition to Leica DFC cameras with the stan dardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayonet adapter To do so instead of the C mount adapter simply use the correspond ing SLR adapter with T2 connection However these third party cameras are not integrated into the Leica Application Suite and have to be operated using the corresponding software from the camera manufacturer The Leica digital cameras are detailed in a sepa rate user manual along with instructions for their assembly and use 45 Video Phototubes HD F amp HDV The video phototubes HD F and HD V are opti mized for adapting state of the art digital cameras to obtain perfect results for image acquisition The video phototubes can be combined with the binocular tubes and the ErgoModules from the Leica ergonomics line A range of high quality video objectives with different self magnifications and C mount allows for the selection of dif
27. ou can simply continue to use your components and add the proven Leica stereomicroscopes User Manual 12 The Modular Design Everything is Relative The Leica M series provides maximum flexibil ity in choosing equipment thanks primarily to the modular configuration and the compatibil ity that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be combined in any way you choose allowing you to create the microscope that best suits your needs Leica M series Despite this you will notice that the controls and individual components do not differ signif icantly Whichever configuration you choose you will quickly feel right at home Have a special request Let us know Leica Microsystems enjoys an exceptional repu tation when it comes to devising customer User Manual specific solutions If you have a special request that cannot be met with standard parts contact your Leica consultant We have a solution for every problem Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series like its predecessors remains compatible with existing series This means that objectives bases tubes and so on can be reused Objectives All objectives of this new generation have the same reference focal length as the objectives of the MZ generation If you prefer you can there fore continue to use the previous Leica objec tive series Leica
28. reventing infections Leica M series User Manual 38 Dioptric Correction All Leica eyepieces are also available with built in dioptric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica M series Using the Dioptric Correction 1 Set the dioptric correction of both eyepieces to the mid position 0 diopter settings While wearing your glasses look through the eyepieces and focus on the specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in direction until the specimen appears sharp Then open the other eye and correct the diopter settings until the image is uniformly sharp User Manual 39 Dioptric Correction and Parfocality Leica stereomicroscopesare parfocally matched The prerequisite for this is the correct setting of the diopters and the parfocality The following adjustments only have to be carried out once by each user Preparation Move the lever of the video phototube to the observation position and open the diaphragm Leica M series Adjusting 1 Set the dioptric correction for both eyepieces to 0 Select the lowest magnification and focus on a flat specimen Select the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces
29. ulations cont d 7 Overview of an M Series Microscope 29 Safety Regulations cont d g The Correct Interpupillary Distance 30 Using the Eyepieces 31 Focusing 32 i 7 Congratulations 12 Changing the Magnification Zoom 34 The Modular Design Everything is Relative 13 Ratchet Steps and Magnification Levels 35 Maximum Compatibility 14 On We Go 15 Eyepieces amp Tubes Magnification Factor of the Eyepieces 37 Assembly Health Notes 38 Base and Focusing Column 17 DioptricCorrection 39 Microscope Carrier 18 Dioptric Correction and Parfocality 40 Optics Carrier q9 Graticules 41 Tube 20 ErgoTube 10 to 50 42 Eyepieces 21 Objective 22 Leica LED5000 CXI Coax Illumination 23 Leica M series User Manual 9 Photography amp Video Photography amp Video 44 Photo Tubes and C mounts 45 Video Phototubes HD F amp HD V 46 Optical Accessories The Different Types of Objectives 48 Leica LED3000 NVI Near Vertical Illumination 50 Leica LED5000 CXI Coax Illumination 51 Leica M50 53 Leica M50 54 Leica M50 55 Leica M60 56 Leica M80 57 Leica M80 58 Leica M80 59 Leica M80 60 Leica M80 61 Leica M series Appendix Calculating the Total Magnification and Field of View Diameter 63 Care Maintenance Contact Persons 64 Care Maintenance Contact Persons cont d 65 User Manual 10 The Leica M series Congratulations The New Leica M Series The introduction
30. wer and unplug the power cable Touching the live circuit can cause injury Transport Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components In order to prevent damage from vibrations disassemble all moving parts that accord ing to the user manual can be assembled and disassembled by the customer and pack them separately Leica M series Installation in third party products Refer to Safety Concept booklet Disposal Refer to Safety Concept booklet Legal requirements Refer to Safety Concept booklet EC Declaration of Conformity Refer to Safety Concept booklet Health risks Workplaces equipped with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthe nopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken optimal arrangement of workplace work User Manual assignments and work flow changing tasks frequently thorough training of the personnel giving consideration to ergonomic and organi zational aspects The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum Direct contact with eyepieces can be a potential
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