998-2300 Revision E TURNER DESIGNS 845 W. Maude Ave
Contents
1. gt gt gt gt MH Mechanical Wiper Pressure sensor P N 2300 360 Factory installed C3 Mechanical Wiper P N 2300 450 Factory installed Note The Pressure sensor and mechanical wiper can only be installed during the manufacturing of the unit Replaceable Brush P N 2200 460 Submersible Battery Kit includes charger P N 2200 601 C3 Submersible Battery Bracket P N 2300 603 Flow Cap P N 2300 700 Shade Cap P N 2300 500 Shade Cap Weight P N 2300 510 Booster Kit P N 2200 900 Note Required for cable lengths greater than 10 meter Extended Cables 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request Secondary solid standard P N 2300 900 for multiple sensors Note Not available for turbidity or ultraviolet sensors C Series Analog Adapter P N 2300 480 Integration Cable P N 2200 160 Note Only used for continuous data output not intended for communication with C3 Submersible Battery 998 2300 Rev E Page 7 C3 Submersible Fluorometer Battery Bracket Flow Cap Shade Cap Booster Kit Booster 1 Locking Solid Standard Insert Secondary Solid Standard 998 2300 Rev E Page 8 C3 Submersible Fluorometer 2 3 Identification Sensors are marked with numbers 1 2 or 3 located adjacent to the optics on the face of the C3 Submersible Fluorometer These numbers correspond to channels2. Battery Lithium lon Protection Features Protects against overcharge overheating short circuiting 998 2300 Rev E Page 31 C3 Submersible Fluorometer Appendix B C3 Submersible Fluorometer Operating Instructions Identification Sensors are labeled 1 2 or 3 adjacent to the optics on the face of the instrument Each channel is factory set in the C3 Windows Software Note The channel labels can be modified in the C Soft software Light Emitting Diode Color Table Do not look directly onto the optics Ultra Violet light can be damaging to the eyes Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a Glass Container for your standards and or samples Note Avoid plastic beakers plastic may fluoresce and might interfere with measurements 2 Place the glass beaker on a non reflective surface preferably black 3 Ensure that the sensor is gt 3 inches above the bottom of the glass beaker 4 Ensure that the sensor is in the center of the glass beaker and has gt 2 inches clearance between the sensor and the inside surface of the beaker 5 Check that the optical surface of the sensor is free of air bubbles Calibrated Sensor gt 2 inches all round On Optical Dark Black Surface Non Reflective 998 2300 Rev E Page 32 C3 Submersible Fluorometer Appendix C C3 Submersible Fluo
3. Programs Accessories Communications HyperTerminal 2 The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C3 Submersible Fluorometer and can be used in the future to establish communication with the instrument quickly 998 2300 Rev E Page 20 C3 Submersible Fluorometer 3 The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK 4 The Port Settings screen will appear Choose the following port settings Bits per second 9600 Data bits 8 Parity None Stop bits 1 Flow control Hardware Click Apply and OK ee COMI Properties Port Settings Bits per second Emai Data bits fe Parity None Stop bits fi Flow control Hardware Restore Defaults coed w 5 Next the HyperTerminal window will appear You are now ready to connect the C3 Submersible Fluorometer to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time C Soft HyperTerminal Pisi x File Edit View Call Transfer Help Dar 13 RhodaminePhycocyani Depth Temp C 90 65 129 70 00 23 71 91 09 130 71 0 00 23 72 15 82 91 01 130 83 0 00 23 72 6 16708 15 07 27 SLAI 130 95 0 00 23 72 6 16 08 15 07 28 91 09 130 95 6 00 23 72 6 16 08 15 07 29 01 61 130 92 0 00 23 72 6 16708 15 07 3
4. C3 Submersible Fluorometer remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 2 3 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected and reconnected please allow 100 seconds for the data stream to begin The C3 Submersible Fluorometer s standard output is in digital ASCH format If the C Series Analog Adapter accessory is used the adapter will convert the standard digital ASCII data output to analog voltage data output for integration with instruments or dataloggers that will accept a 0 5 volt signal C Series Analog Adapter The C Series Analog Adapter PN 2300 480 allows users to convert the standard digital output from the C3 to a 0 5 VDC analog signal for 1 2 or 3 channels installed on a C3 Submersible Fluorometer The voltage output is proportional to the fluorescence intensity measured by the sensors The C Series Analog Adapter is modeled after the C3 s Submersible Battery Pack and is designed to connect directly to the C3 The C3 s Battery Bracket PN 2300 603 is required to hold the C Series Analog Adapter in place and secure it preve
5. at desired location select Set Zero Step 2 Method 2 Select Atmospheric and gravity offset Enter the latitude Step 3 Method 2 Select Save Calibration button Future depth values will be scaled to the offset 3 2 3 Log Setup Screen The Log Setup screen allows the user to configure a logging session activate the mechanical wiper and enable logging The C3 Submersible Fluorometer can log just over 64 000 lines of data Enter a log start date MM DD YY YY and time HH MM SS Enter the sampling interval HH MM SS The minimum sampling interval is 1 second Connected 1 File Settings COMPort Window Help Settings Current Data Log Setup Download Data Log Timing Log Start 10 24 15 06 16 08 Sampling Interval HH MM SS 0 00 30 Wiper Setup Test Wiper Ifthe mechanical wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement 998 2300 Rev E Page 17 C3 Submersible Fluorometer Note If users select revolutions that exceed sampling interval a warning message will be displayed Note Do not click the Wiper Box to enable wiper if the wiper is not installed C Soft Manager E x The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time less revolutions or tu
6. be installed on C3 Submersible Fluorometers with or without the mechanical wiper 998 2300 Rev E Page 24 C3 Submersible Fluorometer Installation 1 Turning clockwise fully hand tighten the Flow Cap housing onto the C3 Submersible Fluorometer The port located on the Flow Cap Housing should be closest to the optical head Turning clockwise hand tighten the End Cap onto the Flow Cap Housing until it is fully tightened Flow Cap Housing End Cap f Cy D aa OUT FLOW PORT a o B IN FLOW Z PORT li R Note Turner Designs recommends the following configuration for the Flow Cap left For optimal performance position the instrument vertically with the sensor head facing upward to expel any air from the system that might cause skewed readings ii Using the Flow Cap as a Calibration Cup l 2 Remove the End Cap gt Use a port plug to plug the port located on the Flow Cap Port Pug Housing Position the unit vertically with optical head facing upward see diagram to the right In this position the Flow Cap Housing can be used to hold standard solutions for calibrating the C3 sensors Note Make sure to completely fill the Flow Cap with solution and thread on the end cap before taking measurements to ensure a darkened environment thereby minimizing interference 998 2300 Rev E Page 25 C3 Submersible Fluorometer 4 5 Using the Secondary Solid Standard The C3 Second
7. 0 DLi2d 131 10 0 00 23 72 Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC 998 2300 Rev E Page 21 C3 Submersible Fluorometer To save data 1 On the HyperTerminal window toolbar choose Transfer and then Capture Text File Edit View Call Transfer Help Send File oel 2 3l Receive File oe capture Text Send Text File Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest Folder C Documents and File SEA TURNERDESIGNSS CAPTURE To ed ele 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File Edit View Call Transfer Help Send File Receive File ma Capture Text Send Text File Pause Resume Capture to Printer T 998 2300 Rev E Page 22 C3 Submersible Fluorometer 4 0 Deployment amp Accessories 4 1 Connecting the Submersible Battery Pack The Battery Pack comes with a charger There is also an optional 12 inch cable P N 105 2590 that is not needed if the battery bracket is installed The battery is fully charged after 8 hours Once the Data Output Ready State is enabled attach the battery to the C3 Submersible Fluorometer with the 12 inch cable or via battery brac
8. 1 2 and 3 in C Soft C3 software Note Channel names can be modified using C Soft Light emitting diode color table Light Source Color Blue Infra Red No Colon Yellow Blue See important note on page 25 Green Ultra Violet No Color Ultra Violet No Color Ultra Violet No Color Optical Brighteners Ultra Violet No Color Do not look directly onto the optics Ultraviolet light can be damaging to the eyes Phycoerythrin Green 3 0 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 Exit all Windows programs 2 Insert the C Soft CD also located on the Turner Designs Software web page http www turnerdesigns com t2 sw main html 3 Open the C Soft software folder and double click on the setup icon 4 The software will automatically be installed in the PC s C Program Files C Soft folder unless user selects alternate location 998 2300 Rev E Page 9 C3 Submersible Fluorometer 5 After restarting the PC double click on the C Soft icon located on the desktop Gott C 5oft C Soft Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1
9. 3 PC Software Interface 1 Connect the 9 pin serial end of the interface cable to the serial port on the back of the PC Note If your computer only has USB ports we recommend purchasing a USB to serial adapter cable See Turner Designs Support web page for recommendations 2 Connect the 8 pin female end of the interface cable to the C3 Submersible Fluorometer s 8 pin male connector Connect the interface cable s 12V pin to the 12V power supply Plug the 12V power supply into a power source Initializing Unit 5 Wait for initialization process to complete Initializing Unit Progress The connection icon located on the upper right of the screen will turn green if connected Note f the connection icon does not turn green disconnect and reconnect power C Soft Manager Connected i File Settings COM Port window Help 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows users to set the time and date identify each optical sensor update serial numbers and enable disable sensors Users can manually set the date and time or choose to synchronize the C3 Submersible Fluorometer to the PC date and time Each channel 1 3 in the Settings Screen has a location associated with it on the C3 Submersible Fluorometer that is numerically labeled 1 3 respectively Channels 4 6 are designated for the C6 Multi Sensor Platform 998 2300 Rev E Page 10 C3 Submersible Fluorometer Click
10. 5 64 Calibrate Chan 6 OM 23 5 Calibrate Depth meters Calibrate Temp C 23 1 View Cabbration Data Start Data Capture Calibration Screen Users can calibrate sensors in either the direct concentration mode raw fluorescence mode or blank subtracted raw fluorescence mode eis Channel Calibration Unit Label Chan 1 Rhodamine Current RFU 83 20 Step 1 Press Set Blank to set the blank Blank RFU value to the current RFU xi Set Blank x10 x100 Step 2 Enter the value of your standard Standard Value units and select the units of measure Step 3 Press Set Standard to set the Standard RFU standard value to the current Set Standard RFU Step 4 Optional Click the temperature Temp Compensation compensation box to activate Step 5 After calibration is complete press 998 2300 Save Calibration Cancel Save Calibration Click to use uncalibrated Raw Fluorescence Mode Rev E Page 13 C3 Submersible Fluorometer Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode is Standard Value units x RFU Response Concentration units Standard RFU Raw Fluorescence Mode Blank Subtracted Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that the
11. Start Date Time End Date Time Records Save 0 a Start Date Time End Date Time Records Save i J J Start Date Time End Date Time Records Save 0 Start Date Time End Date Time Records Save 0 B Start Date Time End Date Time Records Save 0 2 Start Date Time End Date Time Records Save i a i Download Data Clear Datalog 3 3 Enabling Data Output Ready State Users interested in integrating the C3 Submersible Fluorometer into CTDs or Third Party Platforms must enable the C3 s Data Output Ready State using C Soft Software There are five necessary steps to ensure the instrument is properly setup for data output 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensors you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 2 2 2 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note wiping interval is optional and depends on the sampling interval see section 3 2 3 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will 998 2300 Rev E Page 19
12. TURNER D DESIGNS Reliable Instruments for an Unreliable World Submersible Fluorometer May 5 2010 P N 998 2300 Revision E TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 C3 Submersible Fluorometer Table of Contents TIDE OFC ONI U EE soe wasn ieee OE Rs Gea ean O Decade eet AOON 2 1 0 TEV OO COM ieasintisccsiacee T E Oa eave ghia te alsa euro ee eats 5 DOTS PCCIOINGNG SCHIP EE eaten veanss COR chat veslse EEE EA sua c Buy vOineae alan Ronin ec oie sOnedeeaulaes 6 2 1 Instrument Checklisten coarse teabeeseduwe cabs AE AE 6 DO OPONI ACCESSO 9 iia leaa ahaa aa E latte nok ss ue abhouaicen iene eae oh tet a a 7 P Os His sated EE isis elu E APE sete aoe sa A tesa lee EA ren eS oleate Ae toa tes Aaa aah ant 9 PGS TIINC ALLO areren aA banbyeeease AA Na 9 30C Sol Windows SCF TIC ace eruere shat tunes sean viele tats a oii atest 9 3 1 Software Installation and PC Requirements cits aca ssicssuis ooaeevashcobebonsesonssuiiaaioveamass eiabiatavensnes 9 2L M mimon PE Require Men eanan a cane aides 9 h2 Sowar Mtalaa Onenna E acne 9 3 L3 PC SoftWare MUG di CE srna a e a i a 10 3 2 DOL Wale ODEIO soos si csvotvas cat a a a a a anes 10 i Pony Sens S CE RRR ea ee Se ee ee Se ren EN 10 3 2 2 Current Data and Calibration SCreen c ccc ecccceecccesecceseceeeeceeeesseeeeens 11 TURA ID Al E yaoi age eo aie aot tio ae ea EN aan eae 11 PADUS T 1 er Umer RR on reer enn e re mr MTR me
13. ary Solid Standard is designed with a specific filter that provides a stable fluorescent signal that is adjustable The solid standard can be used in place of a primary liquid standard once a correlation between a primary standard and the solid standard has been established It can also be used to check the stability of the instrument and or check for loss in sensitivity resulting from the growth of bio fouling organisms on the sensor optics Installing the Secondary Standard 1 Align Solid Standard Cap numbers with C3 sensor head numbers then snap the Solid Standard Cap in place Solid Standard Cap 2 Slightly rotate cap until it is set into position Note you will feel hear a click when cap is positioned 3 Insert up to 3 Solid Standards into the desired optical port position of the Solid Standard Cap p 4 Rotate the Solid Standards until they set into position 5 If there is a mechanical wiper it must be removed before using the solid standard Individual Solid Standard 1 To adjust each solid standard use the green screwdriver to unscrew the locking nut as far as it will go Adjustable Locking Screw 998 2300 Rev E Page 26 C3 Submersible Fluorometer 2 To change the signal level insert the blade of the green screwdriver through the hole in the locking nut Rotate it until it engages with the adjustment screw that is beneath the locking nut 3 The signal can be adjusted to desired reading by turning t
14. aved in csv format 998 2300 Rev E Page 11 C3 Submersible Fluorometer Tabular Screen Real time data including time date temperature and units are continuously scrolling on the tabular screen for enabled channels Channel names will appear in the associated column headings I f units are not selected during calibration a relative fluorescence units RFU label will appear C Soft Manager 7 E laj x File Settings COM Port window Help Data Table Display Waveform Depth meters 0 00 Phycocyanin RFU 70 72 Rhodamine RFU 69 08 Date Time Settings Current Data Log Setup Download Data 06 16 08 10 34 57 06 16 08 10 34 58 06 16 08 10 34 59 06 16 08 10 35 00 70 40 74 72 0 00 21 48 06 16 08 10 35 01 Current Data Chan 1 Rhodamine 80 44 Calibrate Chan 2 Chlorophyll a o Calibrate 06 16 08 10 35 02 06 16 08 10 35 03 Chan 3 Phycocyanin 76 8 Calibrate 06 16 08 10 35 04 71 76 77 04 0 00 21 48 06 16 08 10 35 05 A TENIA 06 16 08 10 35 06 Chan 4 0 Calibrate 06 16 08 10 35 07 72 48 74 00 0 00 21 47 duce 06 16 08 10 35 08 Chan 5 T f ra t t eine Q Calibrate 06 16 08 10 35 09 74 48 79 60 0 00 21 48 06 16 08 10 35 10 Chan 6 0 Calibrate 06 16 08 10 35 11 06 16 08 10 35 12 Depth meters 0 Calibrat
15. der the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States 998 2300 Rev E Page 28 C3 Submersible Fluorometer For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 5 3 1 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2300 Rev E Page 29 C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste 998 2300 Rev E Page 30 C3 Submersible Fluorometer Appendix A C3 Submersible Fluorometer Specifications Signal Output Digital ASCII or Analog Note analog requires accessory Nonvolatile Memory 64 000 Data Lines Power Draw Max 1 Watt
16. e 06 16 08 10 35 13 73 60 74 40 0 00 21 47 06 16 08 10 35 14 Temp c iS 06 16 08 10 35 15 06 16 08 10 35 16 06 16 08 10 35 17 76 44 73 20 0 00 21 48 06 16 08 10 35 18 06 16 08 10 35 19 06 16 08 10 35 20 78 08 75 12 0 00 21 48 06 16 08 10 35 21 79 04 72 28 0 00 21 48 06 16 08 10 35 22 06 16 08 10 35 23 06 16 08 10 35 24 06 16 08 10 35 25 81 36 73 28 0 00 06 16 08 10 35 26 80 52 72 92 0 00 06 16 08 10 35 27 06 16 08 10 35 28 06 16 08 10 35 29 80 44 76 80 0 00 21 48 View Calibration Data Start Data Capture Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Channel names and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Data can be saved during waveform mode by clicking Start Data Capture 998 2300 Rev E Page 12 C3 Submersible Fluorometer Colt Manager F Fit Settings COMPart Han Settings Curent Data LogSetup Download Data ie a F nA Mal Wna A A Ae i ij i i i ly Vy i ial fi im Current Data yee yyy re hay Chani Chorophylla 15 52 Calibrate Chan 2 Brighteners 1426 Calibrate Chan Fluorescein 35 26 Calibrate Chan 4 ODOM 12 8 Calibrate Chan 5 Phycocyanini 6
17. e TRE AARNE eee HES MIRE pe IRES A A Re 12 WA COR Mi SCO I aeaea tac ventas saline heat sodas NS 12 Canbro O ChEON Gi treiieeuiaiiaiestadeteisrickohoa iheiwie O 13 Direct Concentration MOG siise aiins a e A E A A AE 14 Raw Fluorescence Mode Blank Subtracted ccc ccccceeecceeecceeeeseeeeeees 14 Raw Fluorescence MOde sirere r AEE E A leat oiseauscscnes 14 3 2 2 1 Direct Concentration Calibration eeeseeeseeesecesecesscesseessersrersseesecrseerses 14 Heo BINK 0 syes a a a fries ected aSaealiueen oe tance 14 Step 2 Standard Value and Units ireorieteraen iaa td taalies 14 SED OCES dar aa a eee 15 Step 4 Optional Temperature Compensation esssesssseoeeessssssseseerrsssssssee 15 Step D gt Save Cali PratiOnasis eras u a E 15 3 2 2 2 Raw Fluorescence Calibration Blank Subtracted eee 15 P BANKIN ase rc ead era gence earned anced une a 15 ADS Ope PLCE af Career cent E E 16 Step 3 SKID ANd 20 tO STOD 4viig achat ch ede e ends eau T 16 Step 4 Optional Temperature Compensation ccccccccsssseseeeeeeeeeeeeeeeeees 16 SE oae C AAU OM Gaerne ien cna weenie eee E 16 3 2 2 3 Raw Fluorescence Calibration 0 ccccccccccccccsscccssccesecceeceeesceeeeseeeees 16 3 2 2 4 Depth Calibration Optional ACCESSOTY cccccccsssseeseeeeeeeeeeeaeessseeeeees 16 PATIOS EPIC ONS Ci sess 2a ct vas aawcsceaanrtet yhacteatsiesan bacinaact tutomsanda abuser aauieuaaaleecysartantenaantte 16 Atmosphere and Grav
18. e linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve 3 Graph showing Linear and I S ampl e Linear Region Quenching Regions of the ki Sample Comm sample s response Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll a please refer to the application section of Turner Designs web site at the following URL http www turnerdesigns com esupport understanding html 998 2300 Rev E Page 35
19. he screw Turning the screw clockwise will move the filter closer to the optical head causing an increase in signal 4 Once the desired reading has been obtained the locking nut should be screwed down so that the adjustment screw is held firmly in place Note The response of every solid standard is unique Therefore a new correlation must be determined for every sensor Use the ID space on the Secondary Standard s label to uniquely identify each Standard Important Note Regarding Use of Fluorescein Sensor The Fluorescein sensor when enabled with other sensors specifically Chlorophyll and Rhodamine sensors for simultaneous data collection will produce unreliable results Therefore the Fluorescein sensor should not be used for simultaneous data collection with Chlorophyll or Rhodamine sensors All other sensors can be used simultaneously with the Fluorescein sensor 998 2300 Rev E Page 27 C3 Submersible Fluorometer 5 0 Maintenance and Warranty 5 1 Maintenance 5 1 1 Rinsing The C3 Submersible Fluorometer should be rinsed or soaked in freshwater following each deployment 5 1 2 Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone based grease should be used on the rubber of the male pins of the bulkhead to aid in sealing 5 2 Terms Turner Designs warrants the C3 and accessories to be free from defects in materials and workmanship under normal u
20. ity OMset sra2ss sistcatearece th bared Ohne Msieeeadean et teres 17 32 OS DCU SCLC CM enkenc asstisasumebeca leu tiahiiaatend a aswel 17 3 2 4 Downloading and File Management ccccccccccssseseeseeeeeeeeeeaeseseeeeees 18 3 9 Enabling Data Output Ready State ss secss coisa ree cicncsnas dud nates a a e a 19 C Senes ANd OS AC aplet ions satataicta oie etectanerset eesdateeaetn teed an eet ees 20 Integration Cable for Digital ASCII Output ccccccceceeeessseeeeeeees 20 C3 Submersible Fluorometer 3 3 1 How to setup Hyperterminal for Digital ASCII Data Capture 20 AO DeEDlOVINCNE Se CCOSSOTIOS fc caccleissinorah tend A AEE A 23 4 1 Connecting the Submersible Battery Pack ccccccccccccccccccccessseeseeeeecccececeeeeeaaaaaseseseseseeeeeees 23 A Batory BracketInstala nonen A a 23 4 3 Installing the Mechanical Wipet ccccccccccsssssssssssseeeecceeeeeeeeeeeaeesssssseeeeeeeeeeeeeeeseeaaaeagseeseseeees 23 Bon iE ae A 24 Misah A muanedanmeciasesraadenordseabeaaels 24 AA ATS Cau the FOW ADD esiacidrayon aoire r A A 24 stao E en aenceseh ane laeane ae iaee 23 Using the Flow Cap as a Calibration Cup esesssssssoeeessssssssseerssssssssseerrssss 29 4 5 Using the Secondary Solid Standard csser e a estas nok aks 26 Installing the Secondary Standard nneesseeoeeensessssseeeresssssssseerresssssssseeeeeo 26 Important Note Regarding Use of Fluorescein Sensor ccccccccccsssssss
21. k solution Note A blank solution is a solution without the fluorophore of interest i e de ionized water artificial water or filtered water Note Ensure the optical head is free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Future measurements for this sensor will be blank subtracted based on the blank response recorded during calibration 998 2300 Rev E Page 15 C3 Submersible Fluorometer Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps under the Direct Calibration procedure Step 5 Save Calibration After calibration is complete select Save Calibration Data for the selected sensor will now be in relative fluorescence units and blank subtracted 3 2 2 3 Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen click to use Uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU 3 2 2 4 Depth Calibration Optional Accessory Depth is displayed in meters only There are two calibration options for depth 1 The first uses only the atmospheric pressure as an offse
22. ket Secure C3 Submersible Fluorometer and battery to a fixed structure Data logging will begin after a 100 second delay or at specified time and date Note The battery is rated for 10 000 data lines each data line can range from 3 6 data points 4 2 Battery Bracket Installation The Battery Bracket was designed for easy self contained compact deployment of the C3 Submersible Fluorometer 1 Align the stainless steel pin on the battery bracket with the associated pinhole on the C3 Submersible Fluorometer 2 Apply pressure until the battery bracket is firmly attached the C3 3 Thread the green plastic support line through the holes on the side of the bracket Green Line 4 The line should be flush with the ends of the support line holes on the bracket 5 Slip the battery through the bracket and line up the 8 pin end of the battery with the port on the C3 6 Apply pressure until the battery is firmly attached to the C3 7 Use an Allen wrench to tighten the bracket so it will grip the end of the battery 4 3 Installing the Mechanical Wiper The C3 Submersible Fluorometer s mechanical wiper is an optional accessory that minimizes biofouling during extended deployments The wiper uses non destructive brush material to clean the optics allowing for accurate readings under extreme biofouling conditions Users are able to select the number of wiper rotations before sampling 998 2300 Rev E Page 23 C3 Submersible Fluor
23. nting any break in communication or power The C Series Analog Adapter comes with installation and calibration instructions and customers may download these instructions from the following website http www turnerdesigns com t2 doc Instructions 998 2250 pdf Integration Cable for Digital ASCII Output The Integration Cable is recommended for digital integrations with CTDs or Third Party Platforms This cable is designed so that flow control is disabled therefore it may only be used for integrations and cannot be used for communicating with the C3 As long as the C3 is in the Data Output Ready State power is supplied to the instrument and a 100 second delay has been allowed the C3 will output digital data continuously without interruptions if the integration cable is used Using any other cable for digital integrations may cause interruptions caused by hardware flow control The Integration Cable has 3 connectors 1 A serial DB9 plug RS232 2 A female plug for supplying power to the instrument 3 An 8 pin female connector for connecting to the C3 And can be ordered in either a 2 foot PN 2200 160 or 5 meter PN 2200 165 cable length Note The Integration Cable will have a yellow band which distinguishes it from the standard Interface Cable used to communicate with the C3 Submersible Fluorometer 3 3 1 How to setup Hyperterminal for Digital ASCII Data Capture 1 Onan MS Windows computer open the HyperTerminal program Start All
24. ometer Benefits Limits debris and biofouling organisms from interfering with readings Easy brush replacement design Adjustable wiping revolutions Installation 1 Place the brush assembly on the motor shaft of the C3 Submersible 2 Center the wiper brush between two of the sensors 3 Hand tighten the wiper nut clockwise to lock the brush assembly into position and make sure not to over tighten 4 Open the C Soft Windows software In the Log Setup screen see section 3 2 3 click the Test Wiper button to home the wiper motor The mechanical wiper will make 1 2 revolutions setting the wiper motor shaft to its home position Wiper Nut Brush Assembly Motor Shaft 5 Readjust the wiper brush position by loosening the wiper nut and manually rotating the wiper if necessary 6 Tighten the wiper nut once the wiper brush repositioned 7 Click the Test Wiper button to ensure wiper will home to that position 4 4 Installing the Flow Cap The Flow Cap for the C3 Submersible Fluorometer was designed to enable flow through mode and can be configured with other instruments with online flow through systems The flow cap eliminates the possibility of ambient light interference and it can be used as a calibration cup for holding standard solutions when calibrating optical sensors The flow cap also offers protection for the optical head during deployment or transportation and will help limit biofouling The Flow Cap can
25. on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name The serial number boxes are designed for the C6 Multi Sensor Platform Settings will automatically be saved after exiting the Settings screen Connected Settings Current Data Log Setup Download Data Channel Settings 3 C Soft Manager E Enable Name Serial Chani M Rhodamine E Unit Current Time Chan 10 32 20 Retrieve Unit Time an Chlorophyll a 6 16 2008 Chan 3 MW phycoeysnin Chan 4 Current Time E i Loise2 27 Set Unit To PC Date amp Time Chan 5 6 16 2008 Chan 6 Depth W Manually Set Unit Date amp Time Temp Set Unit Clack 2 2 Current Data and Calibration Screen The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel Current Data Channel names are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button is available for each channel and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be s
26. ors ranging in wavelengths from ultra violet to infrared Each optical sensor is designed with a static excitation and emission filter The C3 Submersible Fluorometer comes with a factory installed temperature probe and can be configured with a pressure sensor for depth measurements up to 600 meters and or a wiper motor to minimize biofouling during extended or short term deployments The C3 Submersible Fluorometer Windows based user interface allows for easy calibration of each sensor digital data reporting data logging file downloading and digital or analog export of data Available Optical Sensors Turbidity In Vivo Chlorophyll a Blue Green Algae Phycoerythrin Blue Green Algae Phycocyanin CDOM Crude Oil Refined Oils and Fuels Fluorescein See important note on page 25 Rhodamine Dye Optical Brighteners gt gt gt gt gt gt A Custom Optics 998 2300 Rev E Page 5 C3 Submersible Fluorometer 2 0 Inspection and Setup 2 1 Instrument Checklist The C3 Submersible Fluorometer package P N 2300 000 includes 998 2300 Rev E C3 Submersible Fluorometer Factory installed temperature sensor Interface cable for communicating with your PC and supplying power to the instrument CD includes C Soft software user s manual and quick start guide emperature Sensor Computer interface cable Page 6 C3 Submersible Fluorometer 2 2 Optional Accessories
27. rn the wiper off Sampling Maximum Interval of Revs 0 1 2 31 40 3 4 J 1 m Cancel After log set up is complete select Enable Datalog see section 3 3 Enabling Data Output Ready State A prompt will appear asking if user is ready to enable logging select Yes Note If data logging is set to start immediately there will be a minimum delay of 100 seconds before startup after power has been supplied 3 2 4 Downloading and File Management Users can download new and exisiting files saved in the C3 Submersible Fluorometer memory Select Download Data Note This step may take up to 10 minutes depending on file size When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in csv format Select Clear Datalog to erase existing data from memory 998 2300 Rev E Page 18 C3 Submersible Fluorometer Users are able to graphically view downloaded data using C Soft software if desired File Settings COMPort Window Help Connected Q C Soft Manager x Settings Current Data Log Setup Download Data Select files to save Total Files 0 Used Data Log Start Date Time End Date Time Records Save l A N R E a as O E e a a A a a a a a a aa a E aaa aa o S a a A ea A a D s De aa A e a a O E aa ae a WA f is 0 10 20 a A S o w Ba D CD
28. rometer Wiring Guide The C3 Submersible Fluorometer outputs digital data in three formats 1 Digital data that can only be read by C3 Submersible Fluorometer s software 2 Digital data in ASCII format that can be read by larger multi parameter systems such as CTD s and 3 Digital data that can be read and converted to analog data by the C Series Analog Adapter An 8 pin impulse cable provides two end connections 1 a 9 pin RS232 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C3 Submersible Fluorometer bulkhead and serial port connectors GOO O Sub D Connector Wire Guide Pin l Out Color C3 Function Corresponding Sub D Connector Pin l Black V BATT Power Connector Cable Center Pin White V BATT Power Connector Cable Housing N A Power ground and V Batt are not common 998 2300 Rev E Page 33 C3 Submersible Fluorometer Appendix D Solid Secondary Standard Use of the Solid Secondary Standard The Solid Secondary Standard can be use to Check signal stability Determine drift e Recalibrate 1 To establish a correlation between a known concentration and its respective fluorescence output immerse the sensor in a sample with a known concentration of your fluorophore 2 Dry off the C3 Submersible Fluorometer s optics attach the Solid Standard and adjust the Solid Standard to produce the same fluorescence output
29. se and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 5 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered un
30. se values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU It is important to note that these values are not blank subtracted or scaled to a standard they are relative values 3 2 2 1 Direct Concentration Calibration Following steps 1 5 will ensure values are reported as blank subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blank solution and ensure the optical head is free from air bubbles Note A blank solution is a solution without the fluorophore of interest i e de ionized water artificial water or filtered water Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Future measurements for this sensor will be blank subtracted based on the blank recorded during calibration Step 2 Standard Value and Units Place the C3 Submersible Fluorometer in a standard solution or use a secondary solid standard see Appendix D for more information on solid standards Note Not all sensors can be used with solid standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not known enter an arbitrary value Future measuremen
31. sseseeeeceeeeeeeeeeaasasaaseseseeseeeeees 2 DO MGiatenanCe Ina Warranty sings A a a EE 28 Dek Ma CMAN GC e a dean tactics e E N 28 BY SAL Mh TERING HNO seca esr Been ago as heed ae he cacad Matted eet Aer art ae hauedel 28 5 1 2 Care for the bulkhead COnneCtOP scvsascsecadeacicestaneiwceseraicdatissiialeadiestianiwcaces 28 BZ NSTI aesete cies nana a e cae onsen E dh Gaduenenaneecee tonics 28 SF Warranty SEL VICE sieran a a asansadnel waaedaciadoeseonod a e a don i aausaten ane Aen atnededonae 28 5 3 1 Out of Warranty Service a sacendcinetseadiencenractsereadoondenndinedseadVendencacraaseadiondinnce 29 Appendix A C3 Submersible Fluorometer SPecifiCQtions ccccccccccccccccceneessceecceeeeaaeseseeceeeeeaaaaseeseeeeeaas 3 Appendix B C3 Submersible Fluorometer Operating INStructiOns cccccccccccccceeeeneseseececeeeaaesseseeeeeaas 32 NGG WUTIG ano aesa parece usa eceds eat 32 Appendix C C3 Submersible Fluorometer Wiring Guide ssnsnnnnnnnneeeesssssssssssssererrrrssssssssssssesees 33 C3 Submersible Fluorometer bulkhead and serial port connectots 33 Appendix D Solid Secondary SIQNOGIG ice iainieciccinteckute wewtsee A E iE E eiaa E udniese temas 34 C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste C3 Submersible Fluorometer 1 0 Introduction The C3 Submersible Fluorometer is manufactured according to user s optical specifications with one two or three optical sens
32. t This is suitable for any shallow application including most fresh water applications 2 The second compensates for gravity variations with latitude as designed from the UNESCO Technical Papers in Marine Science 44 This method assumes 0 C and eae SAUN iix Channel Calibration Unit Label Depth Current Pressure 0 00 PSI Step 1 Press Set Zero to set the Zero Reading atmospheric zero Set Zero Step 2 Select a pressure compenstion method Atmospheric offset only Atmospheric and gravity offset Step 3 When you are done press Save Calibration or press Cancel at Save Calibration Cancel any time to abort this calibration Atmospheric Offset Step 1 Method 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero 998 2300 Rev E Page 16 C3 Submersible Fluorometer Step 2 Method 1 Select Atmospheric offset only Step 3 Method 1 Select Save Calibration button Future depth values will be scaled to the offset Atmospheric and Gravity Offset Step 1 Method 2 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable
33. ts will be calculated based on the correlated response to the standard value Enter manually or select desired units from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode is used 998 2300 Rev E Page 14 C3 Submersible Fluorometer Step 3 Set Standard When readings in Current RFU box have stabilized select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional and allows the user to automatically compensate to the factory installed temperature sensor Temperature compensation is available for Chlorophyll a and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either the Chlorophyll a or Rhodamine from the dropdown menu Data will now be corrected for temperature fluctuations The temperature compensation coefficients for each fluorophore are listed in the table below Fluorophore Coefficient Chlorophyll a 1 4 per degree C Step 5 Save Calibration After calibration is complete select Save Calibration Data for the selected sensor will now be calibrated 3 2 2 2 Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blan
34. value from the sensor as in step 1 Note turning the Secondary Standard adjustment screw clockwise produces a higher signal 3 Now your Solid Secondary Standard has been correlated to a known concentration and as long as it is no longer adjusted stored and used properly that signal will remain a stable reference point 4 Comprehensive information on fluorescence applications can be found at the following website http www turnerdesigns com t2 doc appnotes main html fluorescent Note There are no Solid Secondary Standards available for the Turbidity or UV optical sensors 998 2300 Rev E Page 34 C3 Submersible Fluorometer Linear Range and Quenching The linear range is the concentration range in which the C3 sensor output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in th
Download Pdf Manuals
Related Search