Gel Doc XR+ Bio
Contents
1. for SYBR Green GFP SYBR 170 8199 Image Lab Upgrade for Gel Doc XR System Gold fluorescein includes Image Lab software focus calibration target flat fielding disk installation guide and user manual Included with the imaging system Acrobat and Photoshop are trademarks of Adobe Systems Incorporated Alexa Fluor Coomassie Fluor Orange Qdot SYBR SYPRO and Texas Red are trademarks of Invitrogen Corp Coomassie is a trademark of BASF Aktiengesellschaft Cy is a trademark of GE Healthcare group companies DyLight and Krypton are trademarks of Thermo Fisher Scientific Excel Microsoft and PowerPoint are trademarks of Microsoft Corporation GelGreen and GelRed are trademarks of Biotium Inc Hoechst is a trademark of Hoechst GmbH Mac is a trademark of Apple Inc Mitsubishi is a trademark of Mitsubishi Companies CHEF U S Patent Number 5 549 796 issued to Stanford University is exclusively licensed to Bio Rad Laboratories Inc Practice of the polymerase chain reaction PCR may require a license Precision Plus Protein standards are sold under license from Life Technologies Corporation Carlsbad CA for use only by the buyer of the product The buyer is not authorized to sell or resell this product or its components Bio Rad BIO RAD Laboratories Inc Life Science Web site www bio rad com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600 G Canada 905 364 3435 China 862. 20 8732 2339 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65 roup Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 0508 805 500 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 1404 Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000 Bulletin 5838 Rev C US EG 10 1301 0910 Sig 1109
3. Imaging Gel Doc XR Imager Effortless and Accurate Gel Imaging and Analysis Fast Automated Reproducible Imaging for Perfect Images Every lime For more than two decades the Molecular Imager systems from Bio Rad have been widely recognized and trusted high quality imaging instruments Whether you perform routine imaging of PCR products or protein purifications the Gel Doc XR imaging system will meet your needs Designed for ease of use and an ability to support a wide array of applications it is a perfect fit for individual laboratories multiuser core facilities or research on infectious diseases such as avian flu and H1N1 flu Quality standards of engineering and manufacturing make the system adaptable to academic or biopharmaceutical laboratories Thousands of researchers over the years have published Gel Doc XH system images in dozens of journals and established a record of reliability and excellence for the system The Gel Doc XR system enables quick and easy visualization documentation and analysis of nucleic acid and protein gels blots and macroarrays with a few clicks of the mouse It is a flexible and easy to use system that supports fluorescence and colorimetric detection methods The large imaging area accommodates a broad range of gel sizes from large handcast polyacrylamide gels to small ReadyAgarose gels The Gel Doc XR system is the ideal accompaniment to your PCR and electrophoresis system
4. ftware Automated workflows the entire workflow image capture results report is recorded in a protocol file Protocols can be edited resaved reused and shared among multiple users Allows 10096 repeatability of the workflow and ensures optimized image data and analysis specific to the selected application Auto focus Image Lab software s proprietary algorithms calibrate the system at setup for an automatic focused image at any zoom level Eliminates user error and the need for manual camera adjustments to obtain an image leading to higher image quality Auto camera aperture control you do not have to focus nor adjust aperture setting Only adjust the zoom to position sample Allows you to quickly image across different applications with different aperture setting requirements Flat fielding flat fielding calibrations are performed for each application automatically Delivers image data that is always optimized and reproducible without imaging artifacts for Superior image uniformity and quantification Increased image resolution decreases pixelation when images are cropped or zoomed Allows smooth clean images at any zoom level ee P ee ES Eee i x i554 fs SS EE eee oe e TESI Images can be zoomed in without losing resolution Greater export functionality no need to export images to another program such as Photoshop image edit
5. ing software to change the dpi before importing for publication You can now define your desired resolution within Image Lab software Molecular Biology Applications The Gel Doc XR system can be used in multiple workflows Gel electrophoresis remains an important tool for quantitative analysis of complex protein and nucleic acid samples Applications include nucleic acid isolation or amplification for molecular cloning techniques nucleic acid quality evalution prior to quantitative PCR gene silencing gene modulation and gene expression DNA gel electrophoresis is also used for fine separations of nucleic acid fragments or entire plasmids for analysis cloning transfection environmental testing and quality assurance testing Gel Doc XR systems are suitable for laboratories engaged in RNAi analysis molecular diagnostics epigenetics opharmacogenomics and forensic testing of genomic DNA d N E E coli colony screening by PCR PX M ZI E AZ NH Nl PFGE gel stained with EtBr Safe DNA Imaging Acquire images of DNA electrophoresis samples without exposing samples or users to harmful UV radiation by adding the XcitaBlue conversion screen to your Gel Doc XR system The screen enables detection of DNA with SYBR Safe stain and other blue light excitable fluorescent stains such as GelGreen and SYBR Green Images and analyzed data produced by Gel Doc XR systems are often incorp
6. is supported by the array of analysis tools in the Gel Doc XRt system The gel stained with silver stain shows salmon muscle soybean and rat brain extracts protein mixtures and E coli extracts compared with Precision Plus Protein Unstained standards Capturing Gene Typing Results Gel Doc XR systems are the imagers of choice for pulsed field gel electrophoresis PFGE Gel Doc XR system images generated from CHEF Mapper XA systems for PFGE have been used often by PulseNet International a global network of health and food regulatory agency laboratories coordinated by the Centers for Disease Control and Prevention for strain typing and molecular epidemiology studies Image data and analysis of PFGE gels have contributed to new findings in cancer research food safety public health quality control and genome mapping Protein gels fluorescence stained with Oriole stain top and Flamingo fluorescent gel stain bottom Achieve higher levels of sensitivity with fluorescent stains to differentiate proteins with low expression Resolve closely spaced spots or bands for protein profiling quantitation and characterization Images produced by the Gel Doc XR system have contributed to new findings in many fields This gel shows a strain of Salmonella identified in a food safety experiment Specifications Automation Capabilities Workflow automated selection Application driven user selected or recalled by a protocol Wo
7. oftware lets you devote your valuable time to research and discovery instead of to wondering if you used your imaging system properly Aud ieu mono d acutum aum Li Bimer ond mario aes Dot ett ERO pea emm S haa geb ped Le Kk Ei igi E F LI E Q mox ti d ini D The image stays focused at any zoom point Lane profiles depict band intensity and represent quantities of sample components separated in a gel Results and Reports In addition to printing a picture of your gel for your records Image Lab software creates and prints reports of your experimental data Any part of the report can be copied into popular document processing applications such as Adobe Acrobat and Microsoft Word PowerPoint or Excel files To include a 3 D view of your gel copy it using the onapshot tool and paste it into your presentation slide High quality good looking reports are easy to produce with the combined power of the Gel Doc XR system and Image Lab software When an analysis parameter is changed the results tables are updated instantly to reflect the new data Tutorials With Image Lab software you don t need previous imaging experience to produce optimal gel images Detailed tutorials are accessible via the toolbar and start up page to acquaint you with all of the Image Lab software capabilities 3 4 X 7 8 9 it nm Tutorials for novice users Sophisticated So
8. orated into sample preparation and sample quantitation workflows Ethidium Bromide vs SYBR Safe DNA Stain Detection 18 000 SYBR Safe and XcitaBlue conversion screen m EtBr and UV light 16 000 3 14 000 4 12 000 RU Fluorescence 10 000 8 000 4 6 000 4 4 000 4 2 000 4 0 i B T MUN LES 51 2 25 6 12 8 6 4 32 1 62 0 8 0 4 0 2 0 1 Sample load ng Alternative to UV illumination to better preserve DNA samples Top serial dilutions of EtBr stained precision molecular mass ruler Bio Rad Laboratories Inc on agarose gel imaged with UV light bottom serially diluted precision molecular mass ruler stained with SYBR Safe stain on agarose gel imaged with XcitaBlue conversion screen There is no loss in sensitivity when a combination of SYBR Safe nucleic acid fluorescent stain and less harmful blue excitation is used instead of UV excitable EtBr The image of the gel stained with SYBR Safe stain was taken using the XcitaBlue conversion screen and a filter for SYBR Safe stain and GFP Less harmful detection methods preserve samples better and more efficiently for downstream uses such as cloning Protein gels stained with silver stain top and Coomassie Blue stain bottom Documentation of protein gels for lab notebooks or sample analysis including protein purity and concentration assessment for differential protein expression studies or to monitor gene modulation products
9. rkflow automated execution Controlled by a protocol via application specific setup for image area illumination source filter analysis and reporting Workflow reproducibility 10096 repeatability via recallable protocols from image capture to quantitative analysis and reports Autofocus patent pending Precalibrated focus for any zoom setting or sample height Image flat fielding Dynamic precalibrated and optimized for every application Autoexposure 2 user defined modes intense or faint bands Hardware Specifications Maximum sample size 28 x 36 cm Maximum image area 25 x 26 cm Excitation source Trans UV and epi white are standard 302 nm included with 254 and 365 nm available as options optional trans white self powered or conversion screen optional XcitaBlue UV blue conversion screen Illumination control 3 modes trans UV trans white epi white Detector CCD Image resolution H x V gt 4 megapixels Pixel size H x V 4 65 x 4 65 um Cooling system Not applicable Camera cooling temperature Not available Filter holder 3 positions 2 for filters 1 without filter Emission filters 1 included standard 3 optional Dynamic range gt 3 0 orders of magnitude Pixel density gray levels 4 096 Instrument size L x W x H 36 x 60 x 96 cm Instrument weight 32 kg Operating Ranges Operating voltage 110 115 230 V AC nominal Operating temperature 10 28 C 21 C recommended Operating humidity lt 0 noncondensing U S pa
10. s enabling image analysis and documentation of restriction digests amplified nucleic acids and genetic fingerprinting What the Gel Doc XR System Can Do for You Automated workflow from image to results Makes your gel imaging and analysis quick and effortless Eliminates the need for training Allows any user to repeat the same workflow in exactly the same way Automated image capture Produces beautiful pictures of your gels Delivers quantitative analysis of your protein and DNA samples Automated image optimization Delivers image data that is always optimized and reproducible without imaging artifacts Automated image analysis Produces reports with data organized the way you want a Minimizes time from lab work to presentations gu b Be Gel Doc XR System Applications Nucleic Acid Protein Gel Electrophoresis Electrophoresis Blotting Ethidium bromide Coomassie Blue Colorimetric SYBR Green Copper stain Qdots 525 SYBR Safe Zinc stain Qdots 565 SYBR Gold Flamingo fluorescent gel stain Qdots 625 GelGreen Oriole stain CY2 GelRed Silver stain Alexa Fluor 488 Fast Blast DNA stain Coomassie Fluor Orange DyLight 488 SYPRO Ruby Fluorescein Krypton SYBR Safe stain Coomassie Brilliant Blue R 250 stain Oriole stain Qdot blot Fast Results from a Completely Automated Workflow The Gel Doc XR system is controlled by Image Lab software to automatically and reprod
11. tent 5 951 838 Use the optional XcitaBlue kit if performing SYBR Safe DNA applications the UV to blue conversion screen allows visualization of DNA samples while protecting user and sample from UV radiation damage Catalog Description Catalog Description 170 8195 Molecular Imager Gel Doc XR System with 170 8075 Filter 560DF50 62 mm for SYBR Safe Cy3 rhodamine Image Lab Software PC or Mac includes darkroom 170 8076 Filter GS30BP30 62 mm for SYPRO Ruby Texas Red UV transilluminator epi white illumination camera cables Image Lab software 170 8077 Filter 480BP70 62 mm for Hoechst coumarin Accessories 170 8098 254 nm UV Lamps pkg of 6 170 7950 White Light Transilluminator 170 6887 365 nm UV Lamps pkg of 6 170 8001 White Light Conversion Screen 170 8097 Standard 302 nm UV Lamps pkg of 6 170 8182 XcitaBlue Conversion Screen includes view goggles 170 8089 Mitsubishi PS3DW Printer blue conversion screen for viewing SYBR Green 170 7581 Mitsubishi Thermal Printer Paper 4 rolls SYBR Safe GFP Flamingo and other fluorescent l gel stains 170 8184 Gel Alignment Templates pkg of 3 170 8183 XcitaBlue Conversion Screen and Filter includes view Software goggles and SYBR Safe filter 170 8075 560DF50 170 9690 Image Lab Software PC or Mac for automated blue conversion screen for viewing SYBR Green image capture optimization and 1 D data SYBR Safe and other fluorescent gel stains analysis 170 8074 Filter 520DF30 62 mm
12. ucibly generate gel images Image Lab software is fast taking you from gel to printed results in seconds Your results are visible with a single click of the mouse Image Lab software eliminates the guesswork in imaging You won t have to perform tedious repetitive steps to find the right focus setting You won t have to guess which exposure times will best visualize the bands of interest Image calculations and corrections are done automatically for your application Whether you are working with protein gels nucleic acid gels or your own customized imaging application the Gel Doc XR system will select the proper settings for optimum detection conditions of the stain or label You also won t need to set aside time for training new users the system and the software are easy to use and work together from setup to finish Imaging and image analysis can become the simplest work in the laboratory Simple and Reproducible Image Capture Place your gel on the Gel Doc imager s sample tray and run your protocol Your work is done in just one step Design and save protocols for the imaging steps in your experiments and Image Lab software will run the protocols exactly the same way every time The protocol feature negates variability in results due to different people operating the imaging system When a new project is related to an existing one you can reuse the previously created protocol by revising its parameters and renaming it Image Lab s
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