Models 6400 & 6404
Contents
1. RHt o MP 2 RH 02 The table below represents the range of parameter values Controller Value Output 0 5V Output 4 20mA Temperature Low 0 degrees C 0 0 VDC 4 mA Temperature High 100 degrees C 5 0 VDC 20 mA CO2 Low 0 0 CO2 0 0 VDC 4 mA CO2 High 20 0 CO2 5 0 VDC 20 mA Humidity Low 0 rH 0 0 VDC 4 mA Humidity High 100 rH 5 0 VDC 20 mA O2 Low 0 0 O2 0 0 VDC 4 mA O2 High 20 796 O2 5 0 VDC 20 mA 6400 6404 Series Operations Manual Page 20 of 54 Rev F 09 18 14 SECTION 5 EQUIPMENT OPERATION Once the incubator is properly installed with the humidity pan filled and source connected turn on the power switch on the front upper right side of the incubator When power is applied the incubator will proceed through a series of startup diagnostics After this is complete the incubator will begin to cycle heat and inject CO2 The displays will begin to move toward their setpoints An inner door switch is located on the front of the unit When the outer door is opened the blower and gas inject are disabled Do not manually override the switch The incubator keypad is designed to produce a simple easy to understand user interface that is intuitive and user friendly With just a few presses of the keys the logic behind the user interface is very easy to learn and remember There2. indicates N2 tank 2 has low pressure Continuous Low Reading inlet Display continues to show O2 reading Status message is shown in the message center status menu immediately unless in startup delay To avoid nuisance alarms on startup alarm is delayed until parameter reaches alarm limit the first time 6400 6404 Series Operations Manual Page 48 of 54 Rev F 09 18 14 SECTION 11 SERVICE MODE Your CARON incubator has a built in read only Service Diagnostics mode that can provide information regarding operation of the incubator for troubleshooting purposes There are no adjustments available or necessary in this mode The screens available in this mode allow a service technician to quickly troubleshoot any system level problems that occur with the incubator 6400 6404 Series Operations Manual Page 49 of 54 Rev F 09 18 14 SECTION 12 PREVENTATIVE MAINTENANCE Your CARON incubator is robustly designed to minimize performance problems However regular maintenance is very important for continuous trouble free operation As a general rule CARON recommends an annual calibration check of the temperature humidity optional and CO2 systems CARON offers a full range of on site calibration and validation services We also offer preventative maintenance contracts on our equipment Contact our customer service department for details or visit us on the web at www caronproducts com Recommended Daily Mai
3. LE LE d fe s x 2 8 AC MAIN 5 2 I FAN je TMg d 1080445 BOTTOM HEATER pe 34 DOOR HTR Ha 1 m 33 TRIAC 40A Id 1080445 5 RED BASE HTR Im Ip i 1080434 1 CO2 INCUBATOR MAINBOARD TRIAC 104 14 MAIN HTR TRIAC 1080443 HEATER 1050588 PUMP 37 COLD SPOT xs B j S HEATER 5 29 uv 211 RESERVED 112 DECON Bs 1 1 1 244 5 HA E r p Les 30 k ea 1080418 l a booRswircH 296 5 1080436 DISPLAY BOARD pu kank Rete LCD INTERFACE CO NN CONN LCD DISPLAY MEMBRANE KEYPAD 6400 6404 Series Operations Manual Page 52 of 54 Rev F 09 18 14 SECTION 14 ELECTRICAL SCHEMATICS 6400 4 220 240 VAC 50 60 HZ SINGLE PHASE 3 4 A 785 WATTS oy 102 e103 EQR EL Adaptor 4 SMPS 5 24 VDC 1080426 DC COOLING FAN 1080425 THERMOSTAT S5 pt Sy R Bik 800 1080424 254 9263 16 5 RELAY CONTACT Rum EE Wee mz x z 1080436 ANALOG OUTPUT PRESSURE SW 1080435 RS485 INTERFACE BOARD 1080434 99 2 128 1050775 B CO2INJVALVE sii si SENS Hos ERI ald rie Lise RH CO2 INI 30 4080428 E DECONSLN VALVE 33 eua LIBRA ERR Ls mns 0 DECONSLN 1 89 EBRIELSA TEMP IH TEMP SENSOR NTC THERMIS
4. To change the alarm ring back timer 1 Press the key from the main menu 2 Press the to select Admin 3 Press the to enter Admin 4 Press the select Alarm Ring Back 5 Press the to enter Alarm Ring Back 6 Press the s to adjust the Alarm Ring Back Setting 7 Press the key to save the Alarm Ring Back setting 8 Press the ca Bi key twice to return to the main menu 6400 6404 Series Operations Manual Page 37 of 54 Rev F 09 18 14 Setting the Door Alarm Delay The incubator has a programmable door alarm delay that can be set between 1 to 15 minutes The factory default is 15 minutes To change the door alarm delay time 1 Press the key from the main menu 2 Press the to select Admin 3 Press the to enter Admin 4 Press the select Door Alarm Delay 5 Press the to enter Door Alarm Delay 6 Press the s to adjust the door alarm delay time 7 Press the to save the door alarm delay time 8 Press the 8 key twice to return to the main menu 6400 6404 Series Operations Manual Page 38 of 54 Rev F 09 18 14 Configuring the Temp 2 2 Alarm Relay The incubator is equipped a set of dry alarm contacts Rating of the relay contacts is a maximum of 30V DC 2A A set of NO normally open and NC normally closed contacts are available on the back of the incubator The software can confi
5. are four basic keys to setup or change any incubator parameter to be setup or changed Enter into a submenu when at Main Menu or Exit submenu to return to Main Saves any adjusted parameter Scroll Up Down inside a menu or adjust a selected parameter 6400 6404 Series Operations Manual Page 21 of 54 Rev F 09 18 14 Changing the Temperature Set point The incubator has an operating temperature range of 20 0 C to 60 0 C The lowest temperature set point that the incubator will control temperature is 5 C above ambient A setting of 20 0 C will disable the temperature control and all heaters and alarms will be disabled To change the operating temperature setpoint E key from the main menu Press the to select settings Press the key to enter Settings Press the to select Set Temp Press the key to enter Set Temp Press the to increase decrease temperature setpoint hold for scroll mu Press the E key to save the setpoint Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 22 of 54 Rev F 09 18 14 Changing the 2 Set point The incubator has an operating CO2 range of 0 0 CO2 to 20 0 CO2 A setting of 0 0 CO2 will disable the CO2 control and all CO2 control and alarms To change the operating CO2 setpoint 1 Press the from the main menu 2 Press the s to select settings 3 Press the to enter Settings 4 Press the s to sele
6. both the outer solid and inner glass doors 9 Press and hold the door switch while the calibration counts down for 60 seconds 10 Let go of door switch once the display shows Calibrating XX X 11 Wait until the display reads Calibrating 20 7 12 13 6400 6404 Series Operations Manual Page 29 of 54 Rev F 09 18 14 SECTION 7 ADMIN MODE Many incubator settings are set to common defaults to minimize initial setup of the incubator However these settings are also adjustable to allow a user to customize the incubator These adjustments are all available in ADMIN Mode Setting Over Low Temperature Alarm Level The incubator has both an over and low programmable temperature alarm The factory default for the over temperature alarm is 1 0 C above setpoint The factory default for the low temperature alarm is 1 0 C below setpoint To change either alarm level key from the main menu 2 Press the to select Admin 3 Press the to enter Admin 4 Press the s to select Over Temp Alarm Low Temp Alarm key to enter Over Temp Alarm Low Temp Alarm 6 Press the change alarm level key to save the alarm level key twice to return to the main menu 6400 6404 Series Operations Manual Page 30 of 54 Rev F 09 18 14 Setting High Low CO2 Alarm Level The incubator has both a high and low programmable CO2 alarm The factory default for the high CO2 alarm is 1 0 above 2 setpoi
7. connection tubing with filters stacking adaptors with hardware and spare incubator hardware Choosing a Location This product weighs approx 250 pounds Ensure that sufficient resources are available to safely move the product When moving your incubator do not lift the unit by the door handle or the outer door as structural damage could occur To ensure proper operation you must locate the unit on a firm level surface capable of supporting approximately 250 pounds 500 pounds if stacked The unit should be located in an 18 25 ambient area and where there is no direct airflow from heating and cooling ducts as well as out of direct sunlight Allow four to six inches of clearance on all sides of the product to allow for connections and airflow The unit requires a dedicated electrical connection and a regulated pressurized CO2 supply Choose a location where these facilities are or can be made available Your incubators are designed to stack on top of each other to optimize floor space Stacking brackets are provided with each incubator to secure them together 6400 6404 Series Operations Manual Page 12 of 54 Rev F 09 18 14 Tighten this nut to secure the stacking brackets as shown below TE Tighten this nut to secure the stacking brackets The incubator specifications were developed by testing the product in controlled environment conditions of 72 F to 77 F degrees with a volt
8. kit will need to be installed in the rear access port located on the top left side of the back wall as seen in the picture below If higher humidity levels higher than 90 are required install the brass plug that is supplied in the accessory kit into the rear panel of the cabinet labeled Control Leak This will disable the filtered air exchange With the filtered air exchange disabled the humidity level may increase above saturation resulting in some condensation developing on incubator surfaces 6400 6404 Series Operations Manual Page 14 of 54 Rev F 09 18 14 Filling the Humidity Pan Use only distilled or deionized water with a resistivity between 50KQ CM and 1MQ CM and a neutral pH Using water outside this range will void your warranty To ensure proper operation distilled or deionized water is required in the humidity pan Fill the humidity pan approximately half full of water Place the pan directly under the hinged airflow duct sitting on the bottom floor of the incubator to insure optimum temperature and humidity response The hinged airduct has stops designed into it to ensure the pan is located in the ideal position Slide the humidity pan back under the duct until it hits the stops Connecting a CO supply A High concentrations of carbon dioxide can cause asphyxiation The use of monitors and alarms is recommended for areas where can collect should not contain a siphon tube Install a
9. specification e Cost of express shipment of equipment or parts Any customer modifications of this equipment or any repairs undertaken without the prior written consent of CARON will render this limited warranty void CARON and their representative are not responsible for consequential incidental or special damages whether shipping damage or damages that may occur during transfer to the customer s point of use When the equipment is signed for at the customer s site ownership is transferred to the customer Any damage claims against the shipping company become the responsibility of the customer Repairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use Repair due to the customer s failure to follow normal maintenance instructions Parts considered consumable including light bulbs filters gases etc e Damage from use of improper water quality e Damage from chemicals or cleaning agents detrimental to equipment materials e Force Majeure or Acts of God 6400 6404 Series Operations Manual Page 7 of 54 Rev F 09 18 14 This writing is a final and complete integration of the agreement between CARON and the customer CARON makes no other warranties express or implied of merchantability fitness for a particular purpose or otherwise with respect to the goods sold under this agreement This warranty cannot be altered unless CARON agrees to an alteratio
10. the inner glass door to allow an air sample to be drawn from the incubator If the incubator has just been installed and started up allow 8 12 hours for the incubator to stabilize before making a calibration adjustment If the unit has been in operation allow a full hour after the display reaches the O2 setpoint before making any calibration adjustments After stabilization open the outer door insert the calibration device s sample tube into the port to take a sample key from the main menu 2 Press the to select Calibration 3 Press the to enter Calibration 4 Press the select 2 Sensor to enter O2 Sensor 6 Press the s to increase decrease the O2 Sensor value hold for scroll 6400 6404 Series Operations Manual Page 28 of 54 Rev F 09 18 14 Ambient reference calibration The O2 sensor may be calibrated by using the ambient air as a reference This process involves filling the incubator with ambient air and then setting the O2 calibration reading to the expected O2 level ENT to access the factory configuration setup menu Press the sequence W W 2 Use the s and EE to program 0 3 9 7 as the PIN number 3 Press the select O2 control 4 Press the to enter O2 control 5 Press the select Install 6 Press the key to install O2 control 7 Press the key to skip the 100mV and 4 5V DC calibration prompts 8 Open
11. there are repairs that are not covered under warranty WARRANTY DEFINED Caron Products amp Services Inc herein after CARON hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON COVERED Parts for a period of two 2 years from date of shipment Any part found defective will be either repaired or replaced at CARON s or their authorized representative s discretion Parts that are replaced will become the property of CARON If CARON or their authorized representatives determine that the customer s unit requires further service CARON or the representative may at its sole discretion provide a service technician to correct the problem or require the return of the equipment to the an authorized service depot CARON or their authorized representative will have the right to inspect the equipment and determine the repairs or replacement parts necessary The customer will be notified within a reasonable time after inspection of any costs incurred that are not covered by this warranty prior to initiation of any such repairs NOT COVERED Calibration of control parameters Improper installation including electrical service gas and water supply tubing gas supplies room ventilation unit leveling facility structural inadequacies or ambient conditions that are out of
12. to enter New Pin Press the toggle set Pin number key to save the Pin number value Press the Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 35 of 54 Rev F 09 18 14 Enabling Disabling Audible Alarm The incubator is equipped with an audible and visual alarm The audible alarm can be silenced if an alarm condition occurs by pressing the silence key However the alarm will ring back if the alarm condition is not corrected or a new alarm condition occurs see Alarm Section 11 for more details To enable disable the audible alarm setting 1 Press the key from the main menu 2 Press the 7 to select Admin 3 Press the to enter Admin 4 Press the select Audible Alarm 5 Press the key to enter Audible Alarm 6 Press the toggle between Disable Enable 7 Press the EB key to save the Audible Alarm setting 8 Press the twice to return to the main menu 6400 6404 Series Operations Manual Page 36 of 54 Rev F 09 18 14 Setting the Alarm Ring Back Time The incubator is equipped with an audible alarm ring back timer The ring back timer will begin when an alarm is silenced using the silence key on the control panel When the ring back timer hits the programmed value the audible alarm will sound again reminding the user that the alarm condition needs addressed The factory default for the alarm ring back timer is 15 minutes
13. 2 A The gas supply should be 99 5 pure and stage gas pressure regulator with a maximum adjustment of 25 psi Inlet pressure must be regulated to 15 psi The CO supply should be 99 5 and not have siphon tubes Regulate CO pressure to 15 psi CO tank regulators are available to purchase through CARON customer service Once the cylinder regulator is installed connect the outlet of the regulator to the hose barb fitting using the tubing and clamps provided An inline HEPA filter is provided to remove any contaminants in the CO2 gas supply Check the connections closely for leaks 6400 6404 Series Operations Manual Page 15 of 54 Rev F 09 18 14 If the unit is equipped with an optional built in gas guard system there will be 2 gas inlets Each of the inlets should be connected to an individual gas tank as described above Connecting a supply for suppressed 6404 models The use of monitors and alarms is recommended for areas where is used to suppress oxygen A Low levels of oxygen can cause suffocation The gas supply should be 99 5 and should not contain a siphon tube Install a 2 stage gas pressure regulator with a maximum adjustment of 25 psi Inlet pressure must be regulated to 15 psi A supply should be 99 5 and not have siphon tubes Regulate N pressure to 15 psi tank regulators are available to purchase through CARON customer service Once the cylinder
14. 46 5 cm Electrical 110 130 VAC 50 60 HZ Single 220 240 VAC 50 60 HZ Single Phase 840 Watts Phase 785 Watts Shipping Weight 264 Ibs 120 kg Specifications are subject to change without notice Add 2 75 for handle 6400 6404 Series Operations Manual Page 51 of 54 Rev F 09 18 14 SECTION 14 ELECTRICAL SCHEMATICS 6400 1 110 130V 50 60 HZ SINGLE PHASE 7 0 AMPS 840 WATTS EQR EL Adaptor 4 SMPS 5 24 VDC 1080424 RELAY CONTACT MED 2 7 IM 1080436 ANALOG OUTPUT BOARD 14 1080435 RS485 INTERFACE BOARD RED PRESSURE SW 329 O2N2 INI 28 4050775 02 INJ VALVE 31 2 BOK NN 12 995 LS issuer CO2 IN Bo 1080428 D ECONS SLN VALVE 33 a ia SS 31 Pobre 8S Sst uf PHA Blk rrr TEMPZ NTC THERMISTOR a 02 SENSOR I SENSOR 22 To d a 8 3 m 5 p 1W3GND 3V1 2 EI EQR EL TB DFT LIVE AND NEUTRAL TERMINAL BLOCK Power Supply 110 130 50 60HZ sinde phase 1080433 POWER INLET W FILTER 2 2 RED RED 4 1080421 les EQR EL SW R1 Do MAIN SWITCH i 1080426 uo 1 DC COOLING FAN Bit Ne j TA GN 37 Main Panel Plate BLK RED i ToC 83 1080425 EQR EL GBB G9606 ahinet hamber THERMOSTAT GEREN EDR ay 62 14 SS BU al al 4 5 16H 15
15. CAR amp N 6 CUFT BENCHTOP CO2 INCUBATOR OPERATIONS MANUAL FOR MODELS 6400 1 and 6400 4 6404 1 and 6404 4 Dear Valued Customer Thank you for purchasing CARON Products amp Services equipment We appreciate your business and look forward to being your preferred supplier of controlled environment equipment products in the future At CARON we are committed to continuous quality improvement Our goal is to supply our customers with highly reliable equipment at a fair price In order to openly monitor our performance we would appreciate your feedback on our products and services If you have questions or any suggestions for improvement based on the installation or operation of the equipment you have purchased please contact our service department at service caronproducts com or 740 373 6809 Thanks again for your business 6400 6404 Series Operations Manual Page 2 of 54 Rev F 09 18 14 TABLE OF CONTENTS Section 1 Warranty cio cos ta needa Settee a esta Wadd ain Sa ded edad ede 5 Section 2 Equipment 10 Section 3 Equipment Installation eee eese eee eene 12 Unpacking Choosing a Location Preliminary Cleaning Leveling the Unit Filing the Humidity Pan Connecting a CO2 Supply Connecting a N2 supply Connecting Electrical Power Connecting Alarm Contacts Section 4 Optional Accessory Installation Ope
16. N in Marietta OH Parts that are replaced will become the property of CARON If CARON factory service personnel determine that the customer s unit requires further service dependent of the model involved CARON may at its sole discretion provide a service technician to correct the problem or require the return of the equipment to the factory or authorized service depot CARON will have the right to inspect the equipment and determine the repairs or replacement parts necessary The customer will be notified within a reasonable time after inspection of any costs incurred that are not covered by this warranty prior to initiation of any such repairs NOT COVERED Calibration of control parameters Improper installation including electrical service gas and water supply tubing gas supplies room ventilation unit leveling facility structural inadequacies or ambient conditions that are out of specification Cost of express shipment of equipment or parts Any customer modifications of this equipment or any repairs undertaken without the prior written consent of CARON will render this limited warranty void CARON is not responsible for consequential incidental or special damages whether shipping damage or damages that may occur during transfer to the customer s point of use When the equipment is signed for at the customer s site ownership is transferred to the customer Any damage claims against the shipping company become the
17. TOR H uir 02 SENSOR 121 SENSOR z o 22 4 3 2 xe 2 g A S 00 S ne p S5 Bajas LI VAISALA LED 2 KEYPAD INTERFACE CONN 6400 6404 Series Operations Manual CO2 INCUBATOR MAINBOARD MEMBRANE KEYPAD Power Supply 220 240VAC 50 60HZ single phase 1080433 POWER INLET W EMI FILTER EGR EL TB DFT LIVE AND NEUTRAL TERMINAL BLOCK gt 2 RED R EQRELSW RI MAINSWITCH gZ Cm 2 Lo EQR EL GBB G9606 GROUND BAR 2 613 i4 ji amp BLU AC MAIN UP J FAN JAC 4 J4 DOOR HTR TRIAC 10 h BASE HTR TRIAC 10A 14 MAIN HTR TRIAC 10A 1080438 TOP HEATER 1050588 PUMP B COLD SPOT 23ovAC J o HEATER 19 Jii RESERVED 112 DECON 1 1 1 LL 295 2964 E 1080418 z pooRSwirCH 25 LCD INTERFACE 2 LCD DISPLAY Page 53 of 54 Rev F 09 18 14 DECLARATION OF CONFORMITY In accordance with EN 45014 1998 We based at Caron Products and Services Inc 27640 State Route 7 Marietta OH 45750 USA Declare that the product Equipment Forced Convection CO2 Incubator Model 6400 Oasis CO2 Incubator In accordance with the following directives e 2006 95 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromag
18. age fluctuation of 10 Operating the incubator outside of these specifications will affect the incubator s performance Preliminary Cleaning Your new incubator was thoroughly cleaned prior to leaving the factory It is recommended however to disinfect all interior surfaces with a general purpose laboratory cleaning agent prior to using the product After cleaning dry all interior components with a sterile cloth as necessary Chloride containing cleaners can attack and corrode stainless steel Cleaners with strong acids can damage plastic components 6400 6404 Series Operations Manual Page 13 of 54 Rev F 09 18 14 Before relocating an incubator remove the water in the humidity pan and transfer any product to another incubator Leveling the Unit Place a level on the middle shelf of the incubator The cabinet has built in adjustable leveling feet designed to level the unit Adjust the feet appropriately until the unit sits level left to right and front to back Installing Filtered Air Exchange Port Stopper The Oasis incubator is designed with a filtered air exchange that allows a small amount of ambient air to be drawn into the incubator to slightly reduce the relative humidity to approximately 90 and just below saturation The recirculating fan pulls HEPA filtered ambient air into the incubator and exhausts the air through a HEPA filtered stopper The silicone port stopper HEPA assembly that is supplied in the accessory
19. alarm level key to enter Admin 4 Press the s to select High O2 Alarm Low O2 Alarm to enter High O2 Alarm Low O2 Alarm 6 Press the s to change alarm level 7 Press the key to save the alarm level 8 Press the RES key twice to return to the main menu 6400 6404 Series Operations Manual Page 33 of 54 Rev F 09 18 14 Setting Low RH Alarm Level for unit with optional RH display The incubator will display sound the alarm when the RH level goes below the low RH alarm value The default low RH alarm is 75 To change the alarm level 1 Press the 2 Press the to select Admin 3 Press the EE key to enter Admin 4 Press the s to select Low RH Alarm 5 Press the to enter Low RH Alarm 6 Press the change alarm level 7 Press the 8 Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 34 of 54 Rev F 09 18 14 Setting a System Password PIN It is optional to setup a system password to limit access to the control panel functions If the control panel is protected by a password you cannot go into the menus until the PIN is entered The factory default is 0000 which does not limit access to these menus To setup a system password Press the key from the main menu Press the 7 to select Admin Press the key to enter Admin Press the 4 to select New Pin Press the key
20. arm delays protect the user from nuisance alarms created by routine use such as door openings A list of alarm descriptions the conditions they look for and how they are delayed and displayed are in the table below Alarm Description Condition Delay Audible Alarm Other Notes Detected Visual Rings or Both Back TEMP SENSOR ERROR Faulty Temp Sensor No Delay Both Yes Display shows Heating will Detected be disabled CO2 SENSOR ERROR Faulty CO2 Sensor No Delay Both Yes Display shows CO2 will be Detected disabled RH SENSOR ERROR Faulty Humidity No Delay Both Yes Display shows Sensor Detected OVER TEMPERATURE Temperature reading No Delay Both Yes Heating is disabled Display greater than continues to show Temp Overtemp Alarm reading HIGH CO2 LEVEL CO2 reading greater 15 Minute Delay of Both Yes CO2 injection is disabled than Overtemp Continuous High Reading display continues to show CO2 Alarm reading DOOR OPEN Outer Door Open Programmable 1 15 Both Yes CO2 injection is disabled minutes internal fan shuts down LOW RH Humidity reading Startup Delay amp 30 Both Yes Display continues to show Rh optional Lower than Low Minute Delay of reading Alarm Continuous Low Reading LOW TEMPERATURE Temperature reading Startup Delay amp 15 Both Yes Display continues to show lower than Low Minute Delay of Temp reading Temp Alarm Continuous Low Read
21. ator is equipped with an O2 sensor remove the four screws that hold the O2 sensor in place Replace the sensor plate with the blank sensor plate provided with the incubator Replace the top air duct close the inner and outer doors and press ENTER on the control panel 6400 6404 Series Operations Manual Page 46 of 54 Rev F 09 18 14 The decon cycle is now initiated not open the outer door during the decontamination process The message center on the display will notify you how much time remains to complete the cycle and when the cycle is complete The total time to complete the decon cycle will vary slightly depending upon ambient conditions and line voltage to the incubator The overall time to complete the cycle is approximately 15 hours Heat up time is approximately 2 hours followed by 9 hours of soak time at elevated temperature and humidity The drying cycle lasts 30 minutes followed by a cool down time of approximately 3 hours Upon completion of the decon cycle fill the humidity pan as instructed in the installation instructions Replace the IR sensor reversing the process followed to remove it Replace the O2 sensor reversing the process followed to remove it Press ENTER for the incubator to return to normal operation mode 6400 6404 Series Operations Manual Page 47 of 54 Rev F 09 18 14 SECTION 10 ALARMS A sophisticated alarm system monitors all system parameters for any fault condition At the same time built in al
22. ce the alarm is cleared the contacts will return to their normal position 6400 6404 Series Operations Manual Page 17 of 54 Rev F 09 18 14 SECTION 4 OPTIONAL ACCESSORY INSTALLATION Installing the units on a Roller Base or Floor Stand This product weighs approx 250 pounds Ensure that sufficient resources are available to safely move the product When moving your incubator do not lift the unit by the door handle or the outer door as structural damage could occur Caron offers three different configurations of roller bases and floor stands The units are designed to both add mobility to the incubators and to lift them up off the floor to simplify cleaning underneath them Accessory RLBS401 in the figure below provides swivel casters for added mobility to either single or stacked incubators The two front casters have brakes which can be securely locked once the unit is rolled to its final location When placing stacked units on any roller base or floor stand lock the roller base brakes by pressing down on them lift a single unit onto the base first next lift the second unit onto the first unit Then attach the stacking brackets as shown in the equipment installation section of the manual Accessory FLST401 in the figure below provides adjustable leveling feel for increasing the height of the incubator by approximately 8 to 10 6400 6404 Series Operations Manual Page 18 of 54 Rev F 09 18 14 Accessory FLST402
23. ct Set CO2 5 Press the key to enter Set CO2 6 Press the s to increase decrease CO2 setpoint hold for scroll 7 Press the key to save the setpoint 8 Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 23 of 54 Rev F 09 18 14 Changing the 2 Set point The incubator has an operating O2 range of 20 6 O2 to 1 0 O2 A setting of either 20 7 or 1 0 O2 will disable the O2 control and all O2 control and alarms To change the operating O2 setpoint 1 Press the from the main menu 2 Press the s to select settings 3 Press the to enter Settings 4 Press the select Set 2 5 Press the key to enter Set O2 6 Press the s to increase decrease O2 setpoint hold for scroll 7 Press the key to save the setpoint 8 Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 24 of 54 Rev F 09 18 14 SECTION 6 CALIBRATION After the incubator has stabilized at the user selected setpoints the control systems can be calibrated When in calibration mode the temperature display CO2 display and rh display optional can all be adjusted to match a reference standard Before any system is calibrated it is critical that the incubator has been allowed to stabilize properly In the instructions below each individual system has a recommended stabilization time that is required before calibration Calibrating the Temperature Dis
24. e drying phase the heaters maintain 90 C while a built in pump pulls in HEPA filtered clean air Next the unit cools down to the setpoint from which the decon cycle was initiated The total cycle time is approximately 15 hours The graph below represents the various cycles of the decon cycle Heating Decontamination Drying Cooling down 90 C 60 C 30 C Start End To initiate the decon cycle press the DECON button on the control panel and follow the on screen prompts The first step of the cycle is to replace the water in the pan with 400 mL of water This is the amount of water required to effectively create the moist heat decontamination environment resistivity between 50 and 1 and A Use only distilled or deionized water with a neutral pH Using water outside this range will void your warranty 6400 6404 Series Operations Manual Page 45 of 54 Rev F 09 18 14 You are then prompted to remove the IR CO2 sensor Follow the instructions below to remove the IR sensor equipped during the moist heat decontamination cycle will damage the sensor s f Failure to remove the IR sensor or O2 sensor if Remove the two thumbscrews and lower the top air duct from inside the incubator as shown below Disconnect the cable from the CO2 sensor by rotating the connector counter clockwise and store the sensor in a safe place until it can be replaced following the decon cycle If the incub
25. eM EE 46 Section 11 Service Modo baw ee etna Pech seem 47 Section 12 Preventative 48 Section 13 SpecifIcatlolis inii oo ties 49 Section 14 Electrical 1 50 Appendix A Declaration 52 6400 6404 Series Operations Manual Page 4 of 54 Rev F 09 18 14 SECTION 1 WARRANTY INFORMATION CO2 INCUBATOR LIMITED WARRANTY Please review this section before requesting warranty service At CARON one of our primary goals is to provide customers with high levels of personal service and top quality products delivered on time backed by technical service and supported for the life of the product Before contacting us for warranty service please be aware that there are repairs that are not covered under warranty WARRANTY DEFINED Caron Products amp Services Inc herein after CARON hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON COVERED e Parts and labor for a period of two 2 years from date of shipment Any part found defective will be either repaired or replaced at CARON s discretion free of charge by CARO
26. gure the relay so it changes state during a temperature or CO2 alarm To enable disable a temp CO2 O2 alarm from changing the state of the relay 1 Press the key from the main menu 2 Press the to select Admin 3 Press the to enter Admin 4 Press the to select the desired alarm relay configuration ES key to modify the desired alarm relay configuration 5 Press the 6 Press the to toggle between Disable Enable 7 Press the 3 key to save the alarm relay configuration 8 Press the ca Bi key twice to return to the main menu 6400 6404 Series Operations Manual Page 39 of 54 Rev F 09 18 14 Selecting Language A user may select one of two languages for the control panel display including English and Spanish The default language is English To change the language setting 1 Press the key from the main menu 2 Press the 4 to select Admin 3 Press the to enter Admin 4 Press the select Set Language 5 Press the to enter Set Language 6 Press the toggle between the selections 7 Press the key to save the Set Language value 8 Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 40 of 54 Rev F 09 18 14 Setting System Date Time The incubator is equipped with a real time clock that keeps track of date and time even when power is off Accu
27. in the figure below provides swivel casters for added mobility to either single or stacked incubators The two front casters have brakes which can be locked once the unit is rolled to its final location The unit is designed for a single incubator and elevates it from the floor by approximately 25 _ A pe aa Connecting the CO2 or N2 Gas Guard Accessory GASG401 CO2 backup system and GASG403 N2 backup system provide an optional built in system to allow two regulated CO2 or N2 supplies to connect to an incubator Refer to the equipment installation section connecting a CO2 or N2 gas supply The gas guard is designed to automatically switch from the primary tank to the secondary tank when low gas pressure is detected on the primary tank This allows for a temporary uninterrupted supply of CO2 or N2 to the incubator should the primary tank go empty 6400 6404 Series Operations Manual Page 19 of 54 Rev F 09 18 14 Connecting Analog Outputs Accessory OUTP401 provides terminals at the rear of the incubator to output analog signals representing the temperature CO2 and optional relative humidity levels inside the incubator Connections can be made to in house data acquisition systems to monitor incubator performance The accessory can be configured at the factory to supply either 4 20mA or 0 5V signals that represent the parameters Analog outputs Analog outputs HH
28. ing LOW CO2 LEVEL CO2 reading lower Startup Delay amp 15 Both Yes Display continues to show CO2 than Low 2 Alarm Minute Delay of reading Continuous Low Reading HIGH O2 LEVEL 2 reading greater Startup Delay amp 15 Both Yes Display continues to show O2 than High O2 alarm Minute Delay of reading Continuous High Reading LOW O2 LEVEL O2 reading lower 15 Minute Delay of Both Yes N2 injection is disabled display than Low O2 Alarm Continuous Low Reading continues to show O2 reading ALL CO2 TANKS LOW Optional Gas Guard No Delay Both No Display continues to show CO2 optional indicates both CO2 reading tanks are low CO2 TANK 1 LOW Optional Gas Guard 15 Minute Delay of Both No Solenoid switches to tank 2 optional indicates tank 1 has Continuous Low Reading inlet Display continues to low pressure show 2 reading CO2 TANK 2 LOW Optional Gas Guard 15 Minute Delay of Both No Solenoid switches to tank 1 optional indicates tank 2 has Continuous Low Reading inlet Display continues to low pressure show CO2 reading ALL N2 TANKS LOW Optional Gas Guard No Delay Both No Display continues to show O2 optional indicates both N2 reading tanks are low N2 TANK 1 LOW Optional Gas Guard 15 Minute Delay of Both No Solenoid switches to tank 2 optional indicates N2 tank 1 Continuous Low Reading inlet Display continues to has low pressure show O2 reading N2 TANK 2 LOW Optional Gas Guard 15 Minute Delay of Both No Solenoid switches to tank 1 optional
29. n in writing and expressly stated herein shall be recognized to vary or modify this contract Ohio Law governs this warranty Caron Products amp Services Inc PO Box 715 Marietta OH 45750 740 373 6809 6400 6404 Series Operations Manual Page 8 of 54 Rev F 09 18 14 INTERNATIONAL SYMBOLS AND DEFINITIONS Help Information Warning of hazardous area Warning of hot surface Warning of dangerous electric voltage OPEB POO Earth ground protective conductor WARNINGS Local government may require proper disposal 6400 6404 Series Operations Manual Page 9 of 54 Rev F 09 18 14 SECTION 2 EQUIPMENT OVERVIEW Congratulations You have just purchased the latest technology in CO2 incubators Before using the equipment familiarize yourself with key components of the product and thoroughly read this manual Outer Door Control Switch TRY ay Panel Temp Humidity Power and IR CO2 Switch Sensors Sealed Inner CO2 Sample Glass Door Port Inner Door Adjustable Handle Sliding Shelf 4 B Heated Outer Door Humidity Pan a Leveling Foot 2 6400 6404 Series Operations Manual Page 10 of 54 Rev F 09 18 14 SECTION 2 EQUIPMENT OVERVIEW CONTINUED INCUBATOR CONTROL PANEL e 9 Silence Key Press to Silence audible alarm Alarm Indicator Illuminates on and off during an alarm condition Heat Indicator Illuminates when any system heater is on Injec
30. netic Compatibility Directive and its amending directives Has been designed to comply with the requirements of the following Harmonized Standard e Low Voltage EN 61010 2001 61626 1 2006 Class Vawid By Dave Figel Engineering Production Manager CARON Products amp Services Inc 6400 6404 Series Operations Manual Page 54 of 54 Rev F 09 18 14
31. nt The factory default for the low Temperature alarm is 1 096 below CO2 setpoint To change either alarm level EE to enter Admin 4 Press the s to select High CO2 Alarm Low CO2 Alarm to enter High CO2 Alarm Low CO2 Alarm 6 Press the change alarm level IB to save the alarm level key twice to return to the main menu 6400 6404 Series Operations Manual Page 31 of 54 Rev F 09 18 14 Setting New CO2 Tank for unit without the optional gas backup switch system The CO2 tank depletion reminder automatically calculates how much CO2 gas is left in the tank It alerts the user approximately one week before the gas is depleted which gives the user some buffer time to order new tanks To change CO2 tank net weight and to set new 2 tank Press the 7 to select Admin Press the key to enter Admin Press the 4 to select New CO2 Tank Press the to enter New CO2 Tank Press the to change CO2 tank net weight cu Press the to set new CO2 tank Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 32 of 54 Rev F 09 18 14 Setting High Low O2 Alarm Level suppressed 2 model 6404s only The incubator has both a high and low programmable O2 alarm The factory default for the high O2 alarm is 1 0 above O2 setpoint The factory default for the low Temperature alarm is 1 0 below O2 setpoint To change either
32. ntenance Checks Check the Temperature and CO2 displays versus set points O Check for and correct any alarm condition Check the CO2 and 2 gas tank levels O Check the water level in the humidity pan Recommended Annual Maintenance Checks O Replace inline HEPA filters on both CO2 and N2 gas supplies O Replace O2 sensor O Check tightness of hinge screws on inner and outer door moving parts O Disinfect all interior surfaces with a general purpose laboratory cleaning agent O Perform a complete calibration of the temperature humidity and CO2 and O2 systems O A full validation is recommended for GMP facilities each time a unit is installed moved or undergoes significant repair Contact CARON s service department to schedule on site validation 6400 6404 Series Operations Manual Page 50 of 54 Rev F 09 18 14 SECTION 13 SPECIFICATIONS Humidity Range Elevated up to 95 37 C 0 20 CO2 CO Control 0 1 CO2 CO2 Sensor Infrared CO2 Sensor O2 Range 20 1 O2 O2 Control 0 1 O2 O2 Sensor Fuel Cell Interior Dimensions 20 W x 21 D x 25 H 50 8 cm x 53 3 cm x 63 5 cm Interior Construction Polished Stainless Steel Exterior Dimensions 26 W x 2670 x 36 H 66 cm x 66 cm x 91 4 cm Exterior Construction Powder Coated Cold Rolled Steel Work Space 6 cu ft 170 liters of Shelves 4 Standard 13 Maximum Shelf Construction Perforated Stainless Steel Shelf Dimensions 18 5 x 18 3 47 cm x
33. play Place the temperature reference device in the center of the chamber If the incubator has just been installed and started up allow 8 12 hours for the incubator to stabilize before making a calibration adjustment If the unit has been in operation allow a full hour after the display reaches the temperature setpoint before making any calibration adjustments Press the 2 Press the to select Calibration 3 Press the key to enter Calibration 4 Press the select Temp Sensor 5 Press the to enter Temp Sensor 6 Press the s to increase decrease the Temp Sensor value hold for scroll 7 Press the key to save the Temp Sensor calibration value 8 Press the twice to return to the main menu 6400 6404 Series Operations Manual Page 25 of 54 Rev F 09 18 14 Calibrating the CO2 Display A port is available in the center of the inner glass door to allow an air sample to be drawn from the incubator If the incubator has just been installed and started up allow 8 12 hours for the incubator to stabilize before making a calibration adjustment If the unit has been in operation allow a full hour after the display reaches the CO2 setpoint before making any calibration adjustments After stabilization open the outer door insert the calibration device s sample tube into the port to take a sample 1 Press the Sj 2 Press the 3 Press the to enter Calibration 4 Pre
34. r Setting the Data Log Time Period Press the to enter Data Logging Press the s to select Data Log Period Press the to enter Data Log Period Press the to change the Data Log Period Press the key to save the Data Log Period Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 43 of 54 Rev F 09 18 14 Reviewing Data Logs Time stamped data log information can be viewed on screen and the history scrolled through To review the data logs of a particular parameter key to enter Data Logging 4 Press the s to select Parameter that you want to view key to enter view the data logs 6 Press the s to view the scroll through data logs 7 Press the key twice to return to the main menu 6400 6404 Series Operations Manual Page 44 of 54 Rev F 09 18 14 SECTION 9 USING THE AUTOMATIC DECON CYCLE The incubator is equipped with a built in automatic 90 C moist heat decontamination cycle The cycle is designed to decontaminate the entire interior workspace including all installed components Moist heat at 90 C is proven to effectively kill common organisms known to contaminate incubators The decon cycle consists of a heating phase in which the temperature and humidity rise to approximately 90 C and 60 relative humidity After reaching these temperatures the unit soaks for several hours before entering a drying phase During th
35. rate date and time stamping are necessary for the data logging feature The 24 hour clock is factory set to US Eastern Standard Time To change the date and time 7 8 Press the Press the key from the main menu Press the 7 to select Admin Press the key to enter Admin Press the 4 to select Set Date Time Press the EI key to enter Set Date Time Press the 4 to change the Date Time Date is entered in YYYY MM DD format one digit at a time Time is entered in HH MM SS format one digit at a time Press the to save each Digit of the Date Time value li key twice to return to the main menu 6400 6404 Series Operations Manual Page 41 of 54 Rev F 09 18 14 Resetting Factory Defaults A user can reset factory defaults for temperature and CO2 setpoints and alarm conditions by selecting Reset Defaults To reset defaults Bkey to enter Admin 4 Press the select Reset Defaults key to enter Reset Defaults 03 to again to Reset Defaults Press the E 7 Press the MES key twice to return to the main menu 6400 6404 Series Operations Manual Page 42 of 54 Rev F 09 18 14 SECTION 8 DATALOGGING The incubator is equipped with a built in data logging system The data logger records time stamped records of temperature CO2 and humidity O2 if equipped that can be viewed on screen The information is valuable in determining performance of the incubato
36. ration 17 Installing Incubators on a Roller Base or Floor Stand Connecting the CO2 and or N2 Gas Guard Connecting Analog Outputs Section 5 Equipment 20 Changing the Temperature Set point Changing the CO2 Set point Changing the O2 Set point Section 6 Gallbrattohi usu dox ora DE edo mera E vau dus 24 Calibrating the Temperature display Calibrating the CO2 display Calibrating the O2 display Calibrating the Relative Humidity display Section 7 Admin Mode coe ace Cove nul dec x nya uat kai eR dv udo Fire 28 Setting the Over Low Temperature Alarm Setting the High Low CO2 Alarm Setting New CO2 Tank Setting the High Low O2 Alarm Setting the Low RH Alarm Setting a System Password Enabling Disabling Audible Alarm Setting the Alarm Ring Back Time Setting the Door Alarm Delay 6400 6404 Series Operations Manual Page 3 of 54 Rev F 09 18 14 Configuring Temp 2 O2 Alarm Relay Selecting Language Setting System Date Time Resetting Factory Defaults Section 8 plene Qua tieu nrw ota rt iia Hi 41 Setting the Data Log Time Period Reviewing Data Logs Section 9 Using the Automatic Decon 43 Section 10 ATarmms iueo Cou e xe cx ues nex uius eene
37. regulator is installed connect the outlet of the regulator to the hose barb fitting using the tubing and clamps provided An inline HEPA filter is provided to remove any contaminants the gas supply Check the connections closely for leaks If the unit is equipped with an optional built in gas guard system there will be 2 gas inlets Each of the inlets should be connected to an individual gas tank as described above 6400 6404 Series Operations Manual Page 16 of 54 Rev F 09 18 14 Connecting Electrical Power Connect each incubator to a dedicated grounded circuit Failure to do so could result in electrical shock Ensure that there is unobstructed access to the main power plug disconnect Model 6400 1 requires a 100 130 VAC 50 60Hz 15A power connection The power cord connection is a NEMA 5 15P plug Model 6400 4 requires a 220 240 VAC 50 60Hz 16A power connection The power cord connection is a CEE 7 7 plug When the required electrical connection is available plug the provided power cord into the unit and the electrical outlet Connecting Alarm Contacts A set of dry relay alarm contacts are provided standard with each incubator They are located on the back of the incubator The terminals on the relay contact allow for a NO normally open output a NC normally closed and COM common connection In the event of an alarm condition or power failure the NO contact will close and the NC contact will open On
38. responsibility of the customer Repairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use Repair due to the customer s failure to follow normal maintenance instructions Parts considered consumable including light bulbs filters gases etc Damage from use of improper water quality Damage from chemicals or cleaning agents detrimental to equipment materials Force Majeure or Acts of God 6400 6404 Series Operations Manual Page 5 of 54 Rev F 09 18 14 This writing is a final and complete integration of the agreement between CARON and the customer CARON makes no other warranties express or implied of merchantability fitness for a particular purpose or otherwise with respect to the goods sold under this agreement This warranty cannot be altered unless CARON agrees to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract Ohio Law governs this warranty 6400 6404 Series Operations Manual Page 6 of 54 Rev F 09 18 14 EQUIPMENT INTERNATIONAL LIMITED WARRANTY Please review this section before requesting warranty service At CARON one of our primary goals is to provide customers with high levels of personal service and top quality products delivered on time backed by technical service and supported for the life of the product Before contacting your distributor for warranty service please be aware that
39. ss the select 2 Sensor 5 Press the to enter CO2 Sensor 6 Press the s to increase decrease the CO2 Sensor value hold for scroll 7 Press the key to save the CO2 Sensor calibration value 8 Press the 6400 6404 Series Operations Manual Page 26 of 54 Rev F 09 18 14 Calibrating the Relative Humidity Display If your incubator is equipped with an optional humidity sensor and display the humidity system can be calibrated If the humidity option was not purchased the calibration menus will not be displayed in the calibration mode Place the relative humidity reference device to which the incubator temperature will be calibrated to in the center of the chamber If the incubator has just been installed and started up allow 8 12 hours for the incubator to stabilize before making a calibration adjustment If the unit has been in operation allow a full hour after the temperature display reaches the temperature setpoint before making any calibration adjustments Press the to enter Calibration Press the s to select Rh Sensor Press the key to enter Rh Sensor Press the s to increase decrease the Rh Sensor value hold for scroll key to save the Rh Sensor calibration value key twice to return to the main menu 6400 6404 Series Operations Manual Page 27 of 54 Rev F 09 18 14 Calibrating the O2 Display Independent device calibration A port is available in the center of
40. t Indicator Illuminates when gas 15 being injected Temperature Humidity Display CO2 O2 Display 000000 6400 6404 Series Operations Manual 9 000000 Message Center Displays incubator setpoints and any alarm conditions Menu Select Allows user to select programming menu Enter Allows user to enter any adjusted system variable Allows user to adjust system variables up and down Active Indicator Illuminates when incubator is in Decon Cycle Decon Press to initiate decon cycle Page 11 of 54 Rev F 09 18 14 SECTION 3 EQUIPMENT INSTALLATION Unpacking Your new unit has been thoroughly packaged to avoid shipping damage However the unit should be fully inspected upon arrival before signing for receipt If the package has visual damage make notes accordingly on the freight bill and have it signed by the delivery company In the event of concealed damage after the unit is uncrated keep the carton and packaging material Call the shipping company within 7 days of receipt request inspection and retain a copy of the inspection report For added convenience your incubator was shipped with shelving and internal components installed Remove all foam protective pieces from both outside and inside the incubator Remove any tape holding components in position during shipping A boxed shipping kit is also included inside the incubator The shipping kit includes a user s manual electrical cord gas
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