User`s manual - SI Analytics
Contents
1. debt do een 18 TAUTOTESTE cites cide 19 T DESCRIPTION caia 19 7 2 ACCESS TO THE AUTOTEST 19 8 AB SORBAN OE eee ea anne 21 8 1 ACCESS THE ABSORBANCE 2 nnne 21 8 1 1 Upon starting up 2 22 4 00000000 0 21 8 1 2 From the main menu 0 0 00000010100000000 21 2 i etse corta va cesse cara 21 8 3 MEASURE OE THE cett beth dd 21 8 4 MEASURE OF THE SAMPLE 21 8 5 PRINTING OF RESULTS ici 22 8 6 QUITTING THE MEASURING 22 9 SPEGTIRUM it em 23 10 20 2 rette nennen 25 10 1 KEY IN THE WAVELENGTH iu drenan 25 10 2 KEY IN STANDARD OR FACTOR VALUE naaeasannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 25 10 3 MEASURE OF THE BLANK irere urnana uaan a a a atia ae 26 10 4 MEASURE OF THE STANDARD cccccccecececeeeeeceeeeeeeeeceeeeeeeeeeeeeeeceseceseeeeeeeceeeeeees 26 10 5 MEASURE OF SEVERAL 52. 0 26 10 6 MEASURE OF THE SAMPLE nosvin tiani tinaaa avie a a aida aia 27 10 7 PRINTING OF RESULTS irt voi d a read ii du dag 27 10 8 QUITTING THE MODE ini dada acad d dd oda dn ia 28 SCHOTT INSTRUMENTS UVI LIGHT xs a ea 29 11 1 KEY IN THE 06 29 11 2 PROGRAMMING MEASURE 2 00 29 11 217 MM Al OGL AY coi ita 29 11 2 2 TIME ot Interval ise tr re Dia a decai dia 29 11 2 3 Number of intervals idad nassen EA VE EE FE sh 29 11 3 KEY IN FACTOR OR 5 0 00000 30 11 4 MEASURE OF THE 30 11 5 MEASURE OF THE 5 30 11 6 MEASURE OF THE 5 31 T4 ZPRINTING OF RESULT instet nte a na Rd lr rad Gr a 31 11 8 QUITTING THE KINETICS 32 12 MULTI 00 0000 0 2 33 12 1 2552 d n e d dra 33 12 2 MEASURE OF THE 33 12 3 MEASURE OF THE 5 34 12 4 PRINTING OF RESULTS a bi d cerea tete P per d 34 12 5 QUITTING THE MODE cit en rr e rea ek a da rd dr
3. The lamps the cells and generally any item which appears in the accessories section on the price list Our customers are kindly asked to apply for our consent before returning any instrument for repair No return of materials may be accepted whithout the prior written consent of our Sales Management which will precise the terms of such return If the above consent is given articles shall be returned in their original packaging on a prepaid basis to the following address SCHOTT INSTRUMENTS Hattenbergstra e 10 D 55122 Mainz We reserve the right to reship all instruments received collect failing such consent Whatever method and conditions of transport are chosen for the shipment of the equipment to be repaired under guarantee in the original packaging the corresponding costs and the insurance costs will be payable by the customer Any damage connected to the return transport of the equipment falls within the framework of the guarantee on the express condition that the customer has sent his complaint within forty eight hours by registered letter with acknowledgement of receipt to the carrier A copy of the letter should be sent to SCHOTT INSTRUMENTS For equipment with a guaranty card this is only applicable if the card delivered with the equipment is returned to SCHOTT INSTRUMENTS duly completed SOFTWARE GUARANTEE The software is guaranteed by the designer or the distributor of the software under the conditions specified in t
4. 17 1509001 CERTIFICATE gt o m cc o gt lt gt cc S gt gt x e a ae ZERTIFIKAT CERTIFICATE 22 e anagement Service CERTIFICATE The Certification Body of TUV Management Service GmbH certifies that Schott Instruments GmbH Hattenbergstra e 10 D 55122 Mainz has established and applies Quality Management System for Development production and sale of sensors and analytical instruments An audit was performed Report No 70097265 Proof has been furnished that the requirements according to ISO 9001 2000 are fulfilled The certificate is valid until 2008 07 26 Certificate Registration No 12 100 25805 TMS Munich 2008 08 08 ly TGA ZM 07 62 TUV Management Sereca Gmbt Gruppe Zer werstalle Rdlarstrasse 05 80398 M nchen Dermany 43
5. 12 5 QUITTING THE MODE Use ESC key to quit the Multi WL mode and to return to the main menu 34 UVI LIGHT xs SCHOTT INSTRUMENTS 13 TRANSMISSION From the main menu select Transmittance mode using Upper amp Lower Navigator keys Trans 88 0 540 nm 05223 Printing of results takes place by pressing 7 Example of printed results SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 11 06 2003 5 10 pm 11 06 2003 5 10 pm Mode Transmittance Wavelength 540 nm Sam 001 87 0 0 213Abs Sam 002 23 5 T 0 832Abs Sam 003 17 9 T 1 352Abs 13 1 QUITTING THE MODE Use ESC key to leave the Transmittance mode and to return to the main menu 35 UVI LIGHT xs The menu Methods appears only if at least one method has been saved All parameters of the method displayed can be seen by pressing the Upper amp Lower arrows 4 SCHOTT INSTRUMENTS 14 metHops From the main menu select Methods mode using the MEM key ame AMYLASE Kinetics 7 50 Methods 4 5 4 7 50 Methods 7 50 Methods ACID P 2 gt 4 AMYLASE L 540 nm Factor 12 3 Intervalles 4 Tp inter 30sec 30sec e 0 M The upper line indicates how many methods are in file out of 50 which the file can contain The lower line indicates the method name as well as its file
6. 34 13 0000 0 0 35 13 1 QUITTING THE 0 35 TA METEODS3 san e eb 37 14 1 LOADING A 2 37 14 2 5 37 5iSAVE A METHOD cris 39 16 41 dae d tct aversa dde atu 41 MI 41 6 tarea rad e elu Feind Pa re 41 674952329 A A 41 17 ISO 9001 2 4 4 0000 5 0 0 Ea ANETA 43 UVI LIGHT xs SCHOTT INSTRUMENTS 1 REMARKS Te information contained in this document may be the object of patents or patent applications by SCHOTT INSTRUMENTS The possession of the document in force does not confer any licence rights in and to such patents The following names are SCHOTT INSTRUMENTS trademarks throughout the world SCHOTT INSTRUMENTS UVI LIGHT This guide is updated regularly Updatings are incorporated in the new editions The information furnished by this edition may be amended before the products defined herein become available All Reproduction Rights Reserved Reference Date of last Version Date of document Writer Controller Approving modification 0M8123 06 12 05 11 Nove
7. me Retry or Abandon For any case other than the 15 case call after sales service 6 10 AUTOTEST During the autotest UVI LIGHT displays the type of test in progress None of the problems detected will block the UVI LIGHT You have the choice to either pursue or restart the autotest Before starting the autotest UVI LIGHT displays the type of UVI LIGHT the software version as well as the device serial number UVI LIGHT XS 3 8 S N 218 It is recommended to note these informations down on the manual It will be needed the day you call our authorized servicing network to report a problem 17 SCHOTT INSTRUMENTS 6 11 FLOW CHART AUTOTEST upon turning on ABSORBANCE Enter WL Abs reading CONCENTRATION Enter WL Abs reading KINETICS Enter WL Abs reading MULTI WL Enter WL Abs reading TRANSMITTANCE Enter WL Abs reading CONFIGURATION Enter wavelength Enter standard or factor Reading of blank Reading standard s if needed Reading standard in continuous Register a method Enter wavelength Enter initial delay number and time of interval Enter standard or factor Reading of blank Reading standard s if needed Reading samples Register a method Enter wavelength 1 Enter wavelength 2 Reading of blank Reading samples Printing results Register a method Printing results Choice of language Date amp time set up D
8. permanently amp in real time line of status 540 nm 0 362 15 SCHOTT INSTRUMENTS UVI LIGHT xs 6 3 THE UPPER amp LOWER NAVIGATOR KEYS By using the Upper and Lower arrows you can display different informations according to the menu used Thus you can e move from one mode of the vertical main line to another see chapter 5 11 for example from Absorbance to Configuration e have access to an analysis by name when using Method menu e have the limits to choose displayed upon keying in a number e have the parameters of a method displayed when you are working within the menu Save a method 6 4 THE RIGHT amp LEFT NAVIGATOR ARROWS To inform you that you are given several choices two arrows appear on both sides of the display use Right and Left Navigator Arrows to switch between different options proposed e use ENT to validate e use ESC to return to the main menu without considering the modification in the main menu it allows you to return directly to the Absorbance mode Numerical keypad as well as DEL and keys are not employed in menus except when naming an analysis for the purpose of safeguarding it LANGUAGE N p English 6 5 MEASURE OF THE ZERO Within one measuring mode when UVI LIGHT asks BLANK READY you should reply by ENT to have your choice taken into account In any case you keep the possibility to run the zero by pressing the 0 ke
9. 10 3 MEASURE OF THE BLANK UVI LIGHT requests for the blank The measure of the Blank Ready blank resets the automatic incrementation 340 nm 0 312 of samples Insert the blank then validate by ENT UVI LIGHT does the zero then proceeds to the next step 10 4 MEASURE OF THE STANDARD Insert the standard then validate UVI LIGHT does the measure the display indicates the value of the calculated Factor as well as the concentration at O absorbance Origin 15 3 340 nm 0 1 5 9 Factor 15 13 340 nm 0 159 The calculation done is a linear regression non obligatorily through zero Use ENT key to print the measured values and proceed with sample readings Use DEL key to measure a new standard 10 5 MEASURE OF SEVERAL STANDARDS The straight standard is of linear regression type You have to program at least 2 standards to reach this menu Insert the standard indicated then validate Standard 1 Ready 340 nm 0 000 UVI LIGHT runs the measure displays the standard value Validate to print the value and proceed to the next standard 26 UVI LIGHT xs SCHOTT INSTRUMENTS Repeat the operation until UVI LIGHT displays the value of the calculated factor from the straight regression Use the Right amp Left Navigator arrows to display the ordinate at the Extrapolation limits of the straight standard are 0 origin of the standard curve for the minimal value and 10 beyond the value
10. 40 nm 513 6 No Std tol Factor 0 213Abs 0 832Abs Std 5 0 812 1 352Abs Sam 001 0 213Abs Sam 002 0 832Abs 27 SCHOTT INSTRUMENTS UVI LIGHT xs the case of several standards SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 11 06 2003 5 10 pm 11 06 2003 5 10 pm Mode Concentration wavelength 540 nm No Std 4 Origin 0 025 Correlation 0 717 Factor 16 7 Std 212Abs Std 325Abs Std 421Abs Std 2 519Abs Sam 0 213Abs Sam 0 832Abs 10 8 QUITTING THE MODE Use the ESC key to quit the Concentration mode and to return to the main menu 28 UVI LIGHT xs SCHOTT INSTRUMENTS This step is no longer necessary in preprogrammed method The limits of a value can be displayed at once by pressing Upper or Lower Navigator keys En This step is no longer necessary in preprogrammed method 11 From the main menu select the KINETICS mode with the help of the Upper amp Lower Navigator keys Kinetics 540 nm 0 213 Validate the KINETICS mode by ENT Wavelength 540 nm 0 213 11 1 KEY IN THE WAVELENGTH Modify the wavelength by keying a new value directly with the help of numerical keys this will interrupt the permanent display of value read then ENT Wavelength nm CO UVI LIGHT sets and displays successively 350 nm Setting In
11. GHT sets and displays successively 350 nm setting The display indicates the values of the wavelength and the Absorbance in real time Absorbance 350 nm 0 000 8 3 MEASURE OF THE ZERO The zero blank is done by pressing the 0 key 8 4 MEASURE OF THE SAMPLE Sample measure is done in continuous Display indicates the mode wavelength and Absorbance simultaneously 21 SCHOTT INSTRUMENTS UVI LIGHT xs 8 5 PRINTING OF RESULTS Printing of results is done by pressing 277 key SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 06 11 2003 5 10 pm 11 06 2003 5 10 pm Absorbance Absorbance longueur d onde 540 nm 540 nm Ech 01 0 213Abs e 0 213Abs Ech 02 0 832Abs ei 0 832Abs 03 1 352Abs 1 352Abs 8 6 QUITTING THE MEASURING MODE Use the Upper amp Lower Navigator arrows to exit from the Absorbance mode and proceed to the next measuring mode 22 UVI LIGHT xs SCHOTT INSTRUMENTS 9 SPECTRUM Once in the spectrum mode choose start wavelength and stop wavelength avelength 1 avelength 2 500 nm 540 nm It displays Spectrum 500 nm A 02729 And measures baseline Blank Ready 500 nm 0 719 Blank Spectrum Sampl 1 Ready 500 nm 0 719 The spectrum is automaticaly printed on the external printer for sample 1 Sampl 2 Ready By pressing ENT another spectrum is automaticaly printed on the external printer for sample 2 Example SCHOTT INSTRUMENTS
12. UVI LIGHT XS User s guide SCHOTT Instruments UVI LIGHT xs SCHOTT INSTRUMENTS CONTENTS IEREMARKS n teet ee eri e ef 5 2 40 02 06 7 5 ee 9 4 PRECAUTIONS OF 06 00 0 0000 0 11 5 INSTAEPATION ede a o 13 INSTALLATION MT 13 5 2 FEATURE OF THE NETWORK 2 1 nnn nnn 13 6 GENERAL 0 15 5295 eb cei Deb bee i a pe 15 6 2 INE OFPSTAIE GG tte 15 6 3 THE UPPER amp LOWER NAVIGATOR KEYS nnn nnne 16 6 4 THE RIGHT amp LEFT NAVIGATOR 5 16 6 5 MEASURE OF THE ZERO 2 1 nennt 16 6 6 LIMITS OF CONCENTRATION eene nnne nnn nnn nnn nnn rrr nnn 16 6 7 PRINTING THE RESULTS nn n 16 6 8 FLAGS TO USERS Gatita Cot revente da HE Tav La E N Le E ELI 17 6 9 ERROR MESSAGES da 17 GH O AUTOTESTxX aaa dida 17 FEOW CHART cc
13. UVI LIGHT V1 0 Nr 218 11 06 2003 17h38 ode Spectrum Wavelength 1 500 nm Wavelength 2 540 nm Sampl 01 0 0 205 500 540 23 UVI LIGHT xs SCHOTT INSTRUMENTS EL era ee This step is no longer necessary in preprogrammed method u This step is no longer necessary in preprogrammed method pum M MM 10 CONCENTRATION From the main menu select Concentration mode with the help of Upper amp Lower Navigator keys Concentration 540 nm 0 213 Validate the Concentration mode by ENT Wavelength 540 nm 0 213 10 1 KEY INTHE WAVELENGTH Modify the wavelength by keying a new value directly using numerical keys this will interrupt the permanent display of value read then ENT Wavelength nm w UVI LIGHT sets and displays successively 4 Facteur 350 0 312 10 2 KEY STANDARD FACTOR VALUE Select Factor n Standard using the Right amp Left Navigator keys UVI LIGHT 1108 Validate the choice by ENT in the case of a Factor maximal value 9999 Facteur 15 0 340 0 312 in the case of one Standard maximal range 0 001 to 9999 Etalon 0 0 340 nm 1 L 0 312 SCHOTT INSTRUMENTS UVI LIGHT xs in the case of n Standards 0 001 to 9999 Validate or modify the proposed numerical value last value used or calculated by keying the new value using the numerical keys
14. elay preceeding stand by 18 UVI LIGHT xs UVI LIGHT xs SCHOTT INSTRUMENTS AUTOTEST 7 1 DESCRIPTION Upon starting up the UVI LIGHT these are the successive stages of automatic test e Indication of UVI LIGHT model Indication of the software version number Indication of UVI LIGHT serial number Wavelength recalibration Filter wheel positioning Memory test Internal clock test 7 2 ACCESS TO THE AUTOTEST MODE To go from the stand by mode to the autotest mode simply press any key SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 06 11 2003 17h38 Autotest de mise en route Test lampe Passed Test EEPROM Passed Passed 441 9 476 2 528 7 584 4 684 7 744 2 Passed 19 UVI LIGHT xs SCHOTT INSTRUMENTS pt The displayed values are always the latest used value arbitrarily KED ABSORBANCE 8 1 ACCESS TO THE ABSORBANCE MODE ABSORBANCE 540 NM 0 213 8 1 1 Upon starting up UVI LIGHT After the autotest the Absorbance mode is selected automatically 8 1 2 From the main menu From whatever position of the main menu there are two ways to go back to the Absorbance mode by using the Upper amp Lower Navigator keys by pressing the ESC key 8 2 KEY IN THE WAVELENGTH Modify the wavelength by entering a new value using the numerical keys This will interrupt the permanent display of value read confirm the wavelength by ENT Wavelength 3 nm UVI LI
15. etc The deuterium lamp used in this unit emits UV radiation gt Install the instrument in a ventilated area because it is likely to generate ozone which beyond the limits below can harm health Exposure average value 100 ppb Exposure limits value 200 ppb gt All operations made inside the instrument must be done by SCHOTT INSTRUMENTS or by SCHOTT INSTRUMENTS s authorized technicians 11 UVI LIGHT xs SCHOTT INSTRUMENTS D INSTALLATION 5 1 INSTALLATION All precautions of rigidity and plan it s of the support of the instrument must be taken so that the optic bench didn t undergo any distortion 5 2 FEATURE OF THE NETWORK UVI LIGHT is equiped with an automatic switching adaptor for 230V 50Hz and 115V 60Hz No voltage selection is required before pluging the instrument The plug should comply with the existing norms and should be equiped with an earth wire WARNING THIS DEVICE IS NOT RACCORDABLE TO A NETWORK IT 13 UVI LIGHT xs SCHOTT INSTRUMENTS 6 GENERAL INFORMATION 6 1 THE KEYBOARD The key 0 has a double fonction Numerical entry 0 Zero photometric blank 9 9 9 9 9 1 Return to main menu 5 Numerical keypad 2 Correction of entries 6 Navigation menu 3 backup access to methods 7 Validation of the entries 4 Printing results 8 Back lit screen 2 lines 6 2 LINE OF STATE The second line of the LCD indicates the wavelength and the Absorbance value
16. he literature accompanying the aforementioned software packages Under no circumstances whatsoever will SCHOTT INSTRUMENTS supply any type of guarantee for software packages 27 SCHOTT INSTRUMENTS UVI LIGHT xs By express agreement all damages notably for staff costs lost of earnings business trouble etc are strictly excluded from our guarantee The customer is informed that the software cannot be guaranteed exempt from defects or bugs TRADE SECRET AND PROPERTY RIGHTS This document is protected by a SCHOTT INSTRUMENTS copyright c 2003 and the copying rights are explicitly reserved The software supplied with the equipment or referenced contains trade secrets and confidential information which are SCHOTT INSTRUMENTS s property It is legally protected by the international copyright c laws SCHOTT INSTRUMENTS grants a licence to use its software to the user This may not be disclosed used or duplicated with the intention to save it without SCHOTT INSTRUMENTS s written permission The beneficiary must attach a copy of this document to all authorised partial or total reproductions UVI LIGHT xs If the material is not immediately used it agrees that it is stored in an own and dry place To respect temperatures of storage 10 35 SCHOTT INSTRUMENTS 3 RECEPTION The material SCHOTT INSTRUMENTS has been conceived made tested and inspected while respecting procedures bound to t
17. he norm ISO 9001 ex Receipt The material SCHOTT INSTRUMENTS is inspected carefully before his her its conditioning Since receipt of your device control the state of the packing and if you note an anomaly make in the 48 hours reserves of use close to the carrier Consult then the list of colisage and verify that all is in order Finally if you note that he it misses you something or if the material is damaged DO NOT WAIT CALL SCHOTT INSTRUMENTS To benefit some SCHOTT INSTRUMENTS services cards of applications SCHOTT INSTRUMENTS information technical advices send back us from now on the joined guarantee questionnaire duly completed to the following address SCHOTT INSTRUMENTS HattenbergstraBe 10 D 55122 Mainz GERMANY Phone 49 6131 66 5111 Fax 49 6131 66 5111 E mail support schottinstruments com UVI LIGHT xs SCHOTT INSTRUMENTS PRECAUTIONS OF USE Always make sure that the instrument is connected on the good voltage Between 100 240V 50 60Hz Always disconnect the mains plug before starting any work inside the instrument When dangerous substances for health and environment are used the laboratory or site rules where the instrument is installed must be followed Take all the necessary precautions during the use the instrument to protect the operator from eventual liquids leaks or spills or possible radiations protective gloves anti UV radiation glasses protected clothes
18. it delay 20 350 nm 04312 The display indicates the value of the wavelength and the Absorbance in real time 11 2 PROGRAMMING MEASURE TIME 11 2 1 Initial delay Limit 0 to 240 seconds Init delay 20 Validate or modify the proposed numerical value which 350 nm 0 312 is the lastest value used using the numerical keys by entering the new value directly 11 2 2 Time of interval Limits 1 to 240 seconds Validate or modify the proposed numerical value 350 nm 0 312 which is the lastest value used using the numerical keys by entering the new value directly inter 6 11 2 3 Number of intervals Limits 1 to 25 intervals 29 SCHOTT INSTRUMENTS UVI LIGHT xs Number 5 Validate or modify the proposed numerical value which 350 nm 04312 is the lastest value used using the numerical keys by entering the new value directly 11 3 KEY IN FACTOR OR STANDARD Use factor to calculate enzymatic activity By default it is equal to 1 Use standard to run KINETICS on a standard The OD min is calculated then the corresponding factor is memorized Select Factor or Standard by using the Right amp Left arrows Factor 4 4 Standard 4 340 0 312 340 nm 0 312 Validate the choice by ENT inthe case of a Factor 9999 Max in the case of one standard 11 4 MEASURE OF THE BLANK Blank Ready 340 NM 0 312 Insert the blank then validate UVI LIGHT does the zero proceeds
19. mber 2003 UVI LIGHT xs SCHOTT INSTRUMENTS 2 GUARANTEE The new equipment and material sold by SCHOTT INSTRUMENTS is guaranteed against any manufacturing defects for one year unless otherwise stated by SCHOTT INSTRUMENTS with effect From the technical acceptance of the equipment in the factory by the buyer or his designee or failing this For Metropolitan France from the date on the delivery note For other destinations from the date of factory shipment certified by air waybill consignment note or bill of lading The SCHOTT INSTRUMENTS company guarantee applies exclusively to defectiveness arising from a design fault or from a concealed defect It is strictly limited to the free dispatching of replacement parts except for consumable items or to the repairing of the equipment in our workshops within a deadline of 10 working days shipping delay not included By express agreement the following are strictly excluded from our guarantee All damages notably for staff costs loss of earnings business trouble etc Any breakdown due to an incorrect use of the equipment non adapted mains fall attempt at transformation etc or to a lack of maintenance by the user or to poor storage conditions Any breakdown due to the use of parts not supplied by SCHOTT INSTRUMENTS on SCHOTT INSTRUMENTS equipment Any breakdown due to the transporting of the equipment in packaging which is not its original packaging
20. n Use ENT key to launch a new KINETICS without modifiying the parameters 11 7 PRINTING OF RESULT Printing of results takes place e automatically at the end of the KINETICS e manually by pressing on aF 31 SCHOTT INSTRUMENTS Example of printed result SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 11 06 2003 5 10 pm 11 06 2003 5 10 pm Mode Wave length Delai initial Nombre intervalle Temps intervalle Facteur Ech 01 TO TI T3 0 0 T2 0 0 T4 0 Activit Ech 02 TO T1 T2 T3 T4 Ech Ech 210Abs 230Abs 250Abs 270Abs 300Abs 4 161 317Abs 335Abs 355Abs 370Abs 400Abs kinetics nm 30 secondes 4 25 secondes 13 6 Pl 0 306 minute P2 0 307 minute P3 0 309 minute Pente 0 306 minute 0 016 minute 0 017 minute 0 019 minute 832 352 11 8 QUITTING THE 5 MODE Use ESC to leave the KINETICS mode and return to the main menu 32 UVI LIGHT xs UVI LIGHT xs SCHOTT INSTRUMENTS E This step is no longer necessary in preprogrammed method 12 MULTI WAVELENGTH From the main menu select Multi WL mode using the Upper amp Lower Navigator keys Wavelength 1 540 nm 0 213 Validate the Multi WL mode using ENT Wavelength 1 540 nm 0 213 12 1 KEY INWAVELENGTH Modify wavelength 1 by entering a new value directly using the numerical keys This will interrupt the pe
21. number Methods are classified in alphabetical order Select the method you want using the Right amp Left arrows 14 1 LOADING A METHOD To load the selected test just validate by ENT 7 50 Methods ACID 2 Test parameters are printed automatically The software sets itself in the selected mode ready to do a measure Upon next access to methods the software would be set on the last method loaded 14 2 ERASING A METHOD To erase the pre selected analysis simply validate DEL 7 50 M thodes ACID Bi 2 2 For every suppression of predefined method the software requests confirmation the choice is by default NO Erase ACI UVI LIGHT xs SCHOTT INSTRUMENTS 15 SAVE A METHOD Use MEM key to save a method This key takes the function Protects solely in the following cases If one or several memories remain free If in KINETICS mode the initial delay the number of interval the time of interval are already determined If in Concentration mode the type of calculation is already specified Standard or Factor as well as the value of the Factor concentration AND Absorbance of standard s Then UVI LIGHT requests you to enter the name of the method using 8 letters max At first the name is composed of 8 blanks by default or the preregistered method e Use Right and Left Arrows to modify the letter e Use ESC during operation to quit the menu
22. of Factor 15 3 Origin 0 025 the last standard for the 340 nm 0 159 340 nm 0 159 maximal value Use ENT key to print the measured values and proceed with sample readings Use DEL key to measure a new standard 10 6 MEASURE OF THE SAMPLE Insert sample of unknown concentration Sam 0 0 340 nm 0 000 UVI LIGHT runs the measure in continuous upper display indicates the value of the sample in Concentration Sam 13 2 Use 0 key to redo the 340 nm 0 212 measure of the blank andto initialize the counter of samples to 1 Inthe case of a multi standard concentration values of samples beyond more than 10 to for a new set that of the last standard of the range not accepted the display indicates gt Lin Negative values of concentration are not accepted the display indicates Conc lt 0 10 7 PRINTING OF RESULTS Use the ENT key to print the value of the sample and adding the number of the sample Use the 0 7 key to print the sample without adding the number of the sample it allows to attempt another printing instruction if the software is set up without printer Sam 2 13 2 340 nm 0 212 Example of printed results in the case of a factor in the case of a standard SCHOTT INSTRUMENTS UVI LIGHT V1 0 l SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr Nr 218 218 11 06 2003 5 10 pm 11 06 2003 5 10 pm Concentration Mode Concentration 540 nm wavelength 5
23. rmanenet display of the value read confirm by ENT Wavelength 1 nm w Repeat the above operations to enter the 2nd wavelength 550 nm UVI LIGHT sets and displays successively Blank Ready 550 nm Setting 550 nm 0 312 12 2 MEASURE OF THE BLANK Insert the blank then validate UVI LIGHT does the zero on the 2 wavelengths Blank 550 nm Setting UJ lank 50 nm 0 000 Blank 340 nm Setting Blank 340 nm 0 000 then displays Sam Ready 340 nm 0 000 SCHOTT INSTRUMENTS UVI LIGHT xs 12 3 MEASURE OF THE SAMPLE Insert the sample then validate UVI LIGHT does the measure on the 2 wavelengths Sam 550 nm Setting 550 nm 1 250 340 nm Setting 340 nm 15567 then displays results in ratio and difference of absorbance delta 1 LO2 1 254 550 nm 1 250 1 LO2 0 317 1340 nm 1 567 Values of absorbances of the two respective wavelengths can be displayed using the Right Navigator key Use ENT key to launch a new measure without modifying parameters 12 4 PRINTING OF RESULTS Printing of results takes place automatically at the end of the measure manually by pressing 777 SCHOTT INSTRUMENTS UVI LIGHT V1 0 Nr 218 11 06 2003 5 10 pm 11 06 2003 Mode Multi wavelength Wavelength 540 nm Wavelength 610 nm Sam LO1 0 104 101 102 1 090 127Abs 217Abs 0 284 101 102 0 691 274Abs 965Abs
24. to the next stage then 11 5 MEASURE OF THE STANDARD Standard Ready 340 nm 0 000 Insert the standard then validate UVI LIGHT does the measure the display indicates the value of the calculated Factor Once the total time of KINETICS has elapsed the display indicates the activity as well as the slope in OD minute 30 UVI LIGHT xs SCHOTT INSTRUMENTS Use Right Left Navigator Arrows to display the standard slope Standard 10 0 Standard 10 0 Factor 15 3 Pente 0 310 mn Validate and print the value using ENT Intermediate slope values in OD min can be displayed using the Right Left arrows Standard 10 0 P01 0 305 mn Use ENT key to print the measured values and proceed to sample readings Use DEL key to remeasure the standard 11 6 MEASURE OF THE SAMPLE Sam Ready 340 nm 0 000 Insert the sample and launch the KINETICS using ENT key Delay 027 340 0 123 The initial delay is indicated and time count down started Absorbance value is displayed continuous Once the initial delay has elapsed display indicates time count down for KINETICS The absorbance value is displayed in continuous Sam 1 1103 340 nm 0 513 Once the total time of KINETICS has elapsed display indicates the activity as well as the slope in OD minute Intermediate slope values in OD min can be displayed using the Right Navigator key Sam 1 4 65 Sam 1 4 65 Pente 0 310 Pl 0 305 m
25. without protecting and return to the main menu e Use ENT to validate a letter e Use DEL to modify the previous letter If the name of the method already exists the software asks for confirmation before deleting the previous method the choice is by default NO 39 UVI LIGHT xs SCHOTT INSTRUMENTS 16 coNFIGURATION From the main menu select the Configuration mode using the Upper amp Lower Navigator keys Configuration Configuration Configuration 4 Language Stand by 4 4 Date amp Hour Select the option you want using the Right amp Left Arrows then ENT 16 1 LANGUAGE LANGUAGE Select the option you want among French English and Francais Deutsch using the Right amp Left arrows then ENT Modify values by pressing SUP key or by entering the 26 14 2231 new value of the day directly Validate parameters by 15 27 ENT mb e N gt m mb o gt Z UJ lt Stand by function of the UVI LIGHT permit to sustain the UV lamp s time life Ez Select among the options 15 30 60 minutes and No using bx the Right amp Left Navigator keys then ENT 30 minutes mb o AZ J o N N This option activates the RS232 port for computer communication This function is not stored by default and should be reactivated after each start up 41 UVI LIGHT xs SCHOTT INSTRUMENTS
26. y 6 6 LIMITS OF CONCENTRATION One standard and factor Mode the concentration of a sample is only limited by UVI LIGHT s Absorbance limit 2 500Abs Multi standards Mode the concentration of a sample should not exceed 10 the value of the last standard of the curve 6 7 PRINTING THE RESULTS Printing included method name date and hour A space is reserved for the user s signature or identification in accordance to the requirements of the Good Laboratory Practices It is not intended to print graphs 16 UVI LIGHT xs SCHOTT INSTRUMENTS 6 8 FLAGS TO USERS LCD Problems LCD Line 2 A The Absorbance is superior to 2 500 UVI M Blinking LIGHT is not linear to all wavelength Blinking Conc lt 0 The value in concentration is negative stin The value in concentration is beyond the _ linearity limit of the straight standard Memory empty No analysis is present in memory Insufisant space for the safeguard of a new Saturated memory analysis The printer doesn t answer Retry ou Abandon 6 9 ERROR MESSAGES LCD Problems LCD Line 2 Lamp or sensor out of order Lmp Pb EEPROM nonaccessible back up or loading Memory problem impossible Clock problem Nonaccessible clock hour and date are not correct WL problem UVI LIGHT s WL doesn t set Retry or Abandon Energy problem The light beam is shifted from the cell axis Retry or Abandon Black probl
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