User guide Alias - Scientific Systems
Contents
1. Figure 5 Alias without cover slide out the cooling cover by pulling it gently towards you You can now place well plates If the cooling option is installed i N it N ET aK TA iN 03 b Sor iN Figure 6 Alias with cooling cover 0060 284 12 User guide Alias Alias autosampler front The Alias sampling compartment houses the following parts Figure 7 Alias sampling compartment Syringe Needle arm Injection valve Valve leak bin Sample compartment Needle wash position Wash liquid bottle o0 fF WD 0060 284 12 Figure 8 Alias with cooling A WO N Tubing guide Wash waste Condensed water leakage Cooling cover User guide Alias 0060 284 12 User guide Alias Alias autosampler back The back of the autosampler has the following items Figure 9 Back of Alias oOoOoooooooooooooo o000000000000000 1 9 pin male connector inputs output 2 9 pin female connector serial interface 3 warning label see Control I O connections on page 54 4 on off switch 5 fusebox 6 power connector 7 cooling fan if cooling option is installed do not obstruct 8 type label 10 0060 284 12 User guide Alias Options The following factory installed options are available for the Alias autosampler Cooling if installed a cooling fan is visible at t2. Next Injection Input default this input will start the next injection sequence After finishing the injection sequence the Alias will wait for the Next Injection Input Freeze input the Alias will freeze the analysis time for the time this input is active If the Freeze Input is activated while the analysis time is not running the Alias will perform all programmed pre injection sample handling sample loop But the Alias will wait with injecting the sample until the Freeze Input is no longer active Stop Input with this input the run of the Alias is immediately aborted Active high Active low Autosanple T Mid i ameamplr 7 l YCC l l aj l 55 0060 284 12 APPENDIX C List of errors User guide Alias The following errors may occur with Alias Tray unit ERROR 294 ERROR 295 ERROR 296 ERROR 297 ERROR 298 Needle unit Home sensor not reached Deviation of more than 2mm towards home Home sensor not de activated Home sensor activated when not expected Tray position is unknown ERROR 303 ERROR 304 ERROR 306 ERROR 307 ERROR 312 ERROR 313 ERROR 315 ERROR 317 ERROR 318 ERROR 319 Horizontal needle position is unknown Horizontal home sensor not reached Horizontal home sensor not de activated Horizontal home sensor activated when not expected Vertical needle position is unknown Vertical home sensor not reached Vertical hom
3. Transport liquid 4 The programmed injection volume is aspirated from the sample vial a Syringe Column E Sample Transport liquid 20 0060 284 12 User guide Alias 5 The sample needle moves back to the transport position A second transport plug is aspirated The sample is quantitatively transported into the loop Transport liquid Somple 6 The injection valve switches to INJECT The sample loop is now part of the HPLC mobile phase flow path sample is transported to the column The analysis timer starts n Syringe O gt Transport liquid Sample The sequence is repeated for each injection 21 0060 284 12 User guide Alias Air segment with uL Pickup injections If an air segment has been programmed it appears at the front of the first plug of transport liquid and at the front of every sample plug In this injection mode the air segment at the front of the sample plug is injected into the HPLC system npo headspace pressure can be applied on vials wells in this mode to avoid sample errors due to air expansion during exchange from the sample vial well to the transport position Needle njection loop Buffer tubing fronspor Sample Air l Fluent Transpor Alr C RYQIAMAA WA SQ wy B Tronsport Sample Eluent Transport 22 0060 284 12 User guide Alias Mix amp dilute A Mix amp Di
4. Sample Aspiration PASA is selectable It is a proven concept that combines high precision with simplicity and reliability no moving around with the sample needle reduced risk for bubbles in the sample line po needle port that wears and contaminates There is only intelligent valve switching and highly accurate syringe control buffer tubing Air pressure N HPLC Pump dy HPLC Column The syringe is used to aspirate the sample from a vial into the sample loop Buffer tubing between the syringe and the injection valve prevents contamination of the syringe Wash solvent is used to remove the sample from the buffer tubing and sample needle to rinse the buffer tubing and sample needle 0060 284 12 User guide Alias Syringe and buffer tubing Two sizes of syringes are available for the Alias 500 and 2500 uL The 2500 uL syringe is installed in the Prep version see Specifications Prep version on page 52 of the Alias The 500 uL syringe is the standard syringe combined with the standard 1000 uL buffer and the standard 100 uL sample loop the following injection volume range is available for the various injection modes Fullloop 100 pL Partial loopfill 0 50 uL uL pick up 0 27 uL The maximum injection volumes are calculated with the following formulas Full loop injection volume loop volume Partial loopfill max inj volume x of loop volume uL Pick up max inj v
5. a partial loopfill injection is 1 The initial situation the injection valve is in the INJECT position The sample needle with air needle has entered the vial well Headspace pressure applied through the outer air needle ensures that no air or vapor bubbles are formed during sample aspiration gt Column a Syringe Sample 2 The syringe dispenser aspirates the flush volume from the sample vial to fill the sample line with sample and remove wash solvent 17 User guide Alias 0060 284 12 3 The injection valve switches to LOAD placing a distinct sample plug at the beginning of the sample loop a Column gt loop 5 The injection valve switches to INJECT The sample loop is now part of the HPLC mobile phase flow path sample is transported to the column The analysis starts E a Syinge Sample 18 0060 284 12 User guide Alias If an injection from the same vial and no wash routine is programmed the next injection sequence will start with a flush of 50 of the programmed flush volume Otherwise it will start with a flush of the programmed flush volume If the withdrawal of sample for the next injection exceeds the total volume of the sample buffer tubing the buffer tubing is rinsed before the next injection The next injection will start with the programmed flush Air segmen
6. as listed in the list of accessories and spares appendix D in this manual Replace or repair faulty or frayed insulation of power cords Check actual line voltage to confirm it is the value for which this instrument is wired Make sure power cords are plugged into the correct voltage sources Perform periodic leak checks on supply lines Do not allow flammable and or toxic solvents to accumulate Follow a regulated approved waste disposal program Never dispose of such products through the municipal sewage system 0060 284 12 CHAPTER 2 User guide Alias Introduction to Alias The Alias autosampler is a high throughput autosampler of robust design developed to meet the challenge of the modern analytical laboratory It is a very complete autosampler that needs little bench space the Alias is designed for indoor use The Alias autosampler features among other things PASA injection concept see Injection principles on page 12 High resolution syringe control this ensures very high precision for injection and reagent addition Internal standard addition sample dilution or derivatization can simply be programmed PC control ensures easy to understand operation context sensitive online help is available with every window and dialog Special attention has been paid to ensure a service friendly design To enhance safety speed of operation of the Alias will decrease when the door is opened Optional sample cool
7. be insignificant for the low well plates If the 10 mL vials are used the air needle is lowered pretty far into the vial If the vial is not filled for more than 60 the air needle can be applied as usual The same applies for the deep wells If you need to deviate from these standard settings use one of the optional needle types Which air needle for which titre plate or vial To determine which air needle to use the following dimensions need to be considered the height of the titre plate in mm Ht well depth in mm Dw a or A thickness of capmat or seal in mm Cd PE set needle height in mm Nh EN distance air needle point through the capmat or seal in mm min 2 mm Ac The following must be true Hy Ht Dw must be between 2 and 6 mm If this is true the protrusion length of the sample needle can be calculated this is the distance between the point of the sample needle and the point of the air needle It can i be calculated as as follows Protrusion length Ht Cd Nh Ac You can select the most suitable air needle on the basis of the protrusion length Protrusion length Air needle type from to 50 mm yellow 34 40 56 mm red 28 34 62 mm white 22 28 standard needle 68 mm blue 16 22 74 mm green 10 16 80 mm black 4 10 32 0060 284 12 User guide Alias 10 mL vial 50 mm air needle 2 mL vial 62 mm air needle Greiner deep well M53000 Greiner low well 80 mm air
8. The two inputs can be programmed as Next Injection Input Freeze Input or Stop Input The Next Injection Input Freeze Input and Stop Input can be used to control the Alias by other devices The contact closure output can be programmed as Inject Marker Auxiliary or Alarm output Table IO connector Contact closure output and TTL inputs Pinno Description Cable colors 1 Output Common RED 3 wired 2 Output Normally open BLACK 3 wired 3 Input 1 RED 4 wired 4 Input 2 BLACK 4 wired 5 GND 6 Output Normally closed BROWN 3 wired 7 GND 8 GND ORANGE 4 wired 9 GND BROWN 4 wired 54 0060 284 12 Figure 17 Contact closure output Figure 18 TTL input User guide Alias Contact closure output Inject Marker Output default an Inject marker output will be generated when the injection valve switches from LOAD to INJECT Status duration of the Inject Marker is the same as for the setting for the SparkLink Inject marker pulse Range of the adjustment of the inject marker pulse is 0 1 2 0 seconds Alarm Output the Alarm Output will be activated whenever an error occurs see appendix C for a description of the error codes of the Alias Auxiliary the contact closure output can be used as an Auxiliary which can be programmed on a time base up to 4 times On Off EM 2 Normally closed NC Common COM Normally open NO Contact closure output Vmax 28 Vdc Vac Imax 0 25 A TTL inputs
9. User guide Alias version 1 2 document number 0060 284 12 DECLARATION OF CONFORMITY We Spark Holland BV Pieter de Keyserstraat 8 NL 7825 VE Emmen Declare that the product Autosampler Alias type 840 is in conformity with the following documents e EEC directives 89 392 incl 91 368 and 93 44 machine safety and EEC directives 73 23 and 93 68 low voltage safety applied with the following standard EN 61010 1 Safety requirements for laboratory equipment Class I Installation cat II Pollution degree 2 Spark Holland will not accept any liability for damages directly or indirectly caused by connecting this instrument to devices which do not meet relevant safety standards WARNING f the autosampler is used in a manner not specified by the manufacturer the protection provided by the autosampler may be impaired e EEC directives 89 336 92 31 and 93 68 EMC requirements applied with the following standards Emission EN 61326 1997 A1 1998 A2 2001 A3 2003 Immunity EMC standard for electrical equipmant for measurement control and laboratory use Emission EN 61000 3 2 2000 Electromagnetic compatiblity Part 3 2 Limits Limits for Harmonic current emissions equipment input current 16A per phase Emission EN 61000 3 3 1995 A1 2001 Electromagnetic compatiblity Part 3 Limits Section 3 Limitation of voltage fluctuations and flicker in low voltage su
10. able between the Alias and the power outlet Switch on the Alias On your PC open Alias Service Manager and enter the required settings Connect the drain tubing to the waste outlet Fill the wash solvent bottle inside the sampling compartment of the Alias with distilled water and propanol 80 20 v v or mobile phase Only water or organic solvents should be used Do not use crystalline or buffer solutions as these may block the system and cause severe damage Degas the wash solvent to prevent air bubbles from forming in the syringe 10 Fill the wash solvent tubing syringe and buffer tubing by washing the system two or three times Use 100 IPA for better degassing or removing of air bubbles 11 Check if air bubbles are trapped in the syringe remove them by gently tapping the syringe 44 0060 284 12 User guide Alias 12 Connect your HPLC pump to port 1 of the injection valve and the column or the capillary to port 6 of the injection valve Check for leakage and let the system stabilize for at least 5 minutes ASM software A software package is supplied with the Alias autosampler Alias service manager ASM Alias Service Manager allows you to upload Alias firmware It offers the following functionality You can define a port through which you want to do the update You can access the Communication Settings Direct control Service and Adjustment windows for Alias You can access ASM help and About Exe
11. ample 0060 284 12 User guide Alias 3 The injection valve is switched to LOAD position placing a distinct sample plug at the inlet of the sample loop a a Syringe es Column tc 4 The sample loop is quantitatively filled by transporting a number of times the loop volume through the loop depending on the volume of the loop 3 x loop volume for loop lt 100 uL 2 x loop volume for loops 100 uL 500 uL 1 5 x loop volume for loop 500 uL 0060 284 12 User guide Alias 5 The injection valve switches to the INJECT position The sample loop is not part of the HPLC mobile phase flow path sample is transported to the column The analysis starts A wash routine is performed after each injection Air segment with Full loop injections An air segment of 5 uL can be used to reduce the amount of flush volume This air segment is at the front of the flush volume and will not be injected With a standard needle the flush volumes must be a minimum of 30 uL for injections with air segment and 35 uL for injections without air segment If samples are highly viscous it may be necessary to program larger flush volumes and reduce the syringe speed for better performance Needle Injection loop Buffer tubing A l Sampa Erud Flush Am B Sample Fluent Flush gt L L EEE MA gt 16 0060 284 12 User guide Alias Partial loopfill injections The switching sequence for
12. arious types of titre plates Capmats Syringes 4400 500 Syringe 500 uL 4400 255 Syringe 2500 uL Prep Sample needles 0840 303 STD with Rheodyne connections 0840 304 BIO with Rheodyne connections 0840 313 STD with Valco connections 0840 314 BIO with Valco connections 0840 319 serum sample needle with Valco connections 6 different types of air needles for different heights of titre plates or vials Protrusion length Air needle type from to 0045 503 50 mm yellow 34 40 0045 504 56 mm red 28 34 0045 505 62 mm white standard 22 28 needle 0045 506 68 mm blue 16 22 0045 507 74 mm green 10 16 0045 508 80 mm black 4 10 58 H uL Pickup injections 12 19 A About this guide 1 Air needle replacement 33 Air needles for Alias 11 31 Air segment with uL Pickup injections 22 Air segment with Full loop injections 16 Air segment with Partial loopfill injections 19 Alias autosampler 43 Alias autosampler back 10 Alias autosampler front 8 27 Alias fluid connections 46 Alias tubing 46 Analytical trouble shooting 38 40 ASM software 44 45 C Cleaning 27 Control I O connections 10 54 F Full loop injections 12 14 Fuses 37 Injection principles 4 12 Injection valve and rotor seal 28 Installation 43 Instrument description 5 Instrument errors 38 Introduction to Alias 4 L List of accessories and spares 5 58 List of errors 56 Mainte
13. cute the following steps to install ASM 1 3 Insert the CD into the CD drive of your PC If the autorun feature is active the installation wizard will appear If autorun is not active use the browser to go to the CD drive and double click install exe to start the installation wizard Answer all questions that pop up in the wizard click Next to go to the next step in the installation procedure Click Finish to end the installation procedure The software is now installed Refer to online help of ASM for more information Upload notification If the upload notification is displayed the ASM has discovered a problem in PC settings that prevents correct uploading of the new files This problem may be solved by adapting the size of the serial ports FIFO Execute the following steps to adapt settings 1 Open the Windows Device Manager Configuration settings System settings Communication port In the COM port properties select the Port settings tab Click Advanced Use the sliders to change the size of the receive buffer The default values for Receive buffer high 14 and Transmit buffer high 16 should be sufficient However decrease the values to 8 if buffer overflow errors appear Click OK and close the Windows Device Manager Restart your PC Open ASM and try to upload again 45 0060 284 12 User guide Alias Contact service if there still are problems during uploading Alias fluid connections When all
14. d line first sample position 2C1 last sample position 2C1 destination position 2C7 Reagent A 1C1 Reagent B 1C7 etc The mix method can be executed before the injection method The injection is performed from the destination position when a mix method is programmed and TO DESTINATION is the last step in the mix method The injection is performed from the sample position when a mix method is programmed and TO SAMPLE is the last step in the mix method The mix method can also be executed without an injection method 26 0060 284 12 User guide Alias CHAPTER 3 Maintenance For all maintenance procedures Open the door of the Alias 2 If the cooling option is installed remove the cooling cover by sliding it towards you 3 Press the two buttons at the top sides of the Alias simultaneously Remove the cover by pulling it towards you You need not disconnect the Alias from the power source for any of the maintenance procedures In this way software control will still be possible Use the Direct Control function in the Alias Service Manager ASM software to check operation of the various parts of the autosampler Cleaning In general the Alias autosampler needs very little maintenance You can clean the outside with a damp cloth with non aggressive cleaning liquid Other items that may need periodic cleaning valve leak bin see Alias autosampler front on page 8 a special leak bin is installed undernea
15. dle unit use the tubing guide integrated in the leakage drain N 47 0060 284 12 User guide Alias Waste tubing Make the following connections for disposal of waste liquids General waste connect the drain tubing in the shipkit of the Alias to the right hand drain hose connector see figure 8 number 2 Place the other end in a bottle for waste on the floor Through this drain all the liquid dispensed to the wash position is removed Sample liquid that is not injected is also removed through this tubing Condensation water and leakage drain through the left hand hose connector see figure 8 number 3 all leaked solvents and condensation from cooling are drained If the cooling is used you are advised to connect this hose connector in the shipkit of the Alias to a waste container on the floor Make sure that none of the drain or waste tubes is twisted this might obstruct the flow path Wash solvent and syringe rinse Use a clean bottle for the wash solvent and place it on the left hand side of the Alias You are recommended to use a mixture of distilled water and isopropanol 80 20 or mobile phase as wash solvent Before using the wash solvent degas the solvent with helium or an ultrasonic bath Do not use salts or buffer solutions crystals may block or damage the system To fill the wash solvent tubing execute the following steps 1 Place the end of the wash solvent tubing in the fil
16. e pre wontons 40 Installation 43 Alas autosampler 52d neenon eel Cue atten 43 A NAUETINPICHN EI ELEM 45 Alias fluid Connections oh ere ro e dede qe t d a And ho Ra e te rt Y ce ERU 46 Specifications 50 Specifications Prep version ederet ed erede Reda dates o PL ed eet da a EEEa dete de e de anian 52 Control I O connections 54 List of errors 56 List of accessories and spares 58 0060 284 12 User guide Alias Index 59 0060 284 12 CHAPTER 1 User guide Alias About this guide This guide is written for laboratory technicians who use the Alias autosampler for execution of analytical runs This guide offers the following information Chapter 2 describes the Alias autosampler and injection principles Chapter 3 describes maintenance procedures for the Alias autosampler Chapter 4 describes trouble shooting Chapter 5 describes installation and upgrading procedures for the Alias autosampler and Alias Service Manager software Appendix A lists specifications of the Alias autosampler Appendix B provides information on Control I O Appendix C lists error messages Appendix D lists accessories and spare parts available for the Alias autosampler An index has been provided for easy reference 0060 284 12 User guide Alias Pictorials used in this manual The following pictorials are used in this guide gt WARNING gt CAUTION 29 T
17. e sensor not de activated Vertical stripper did not detect plate or wash waste Vertical stripper stuck Vertical The sample needle arm is at an invalid position Syringe dispenser unit ERROR 324 ERROR 330 ERROR 331 ERROR 334 ERROR 335 Injection valve Syringe valve did not find destination position Syringe home sensor not reached Syringe home sensor not de activated Syringe position is unknown Syringe rotation error ERROR 340 ERROR 341 ERROR 342 Destination position not reached Wear out limit reached Illegal sensor readout Cooling unit ERROR 347 Temperature above 48 C at cooling ON 56 0060 284 12 User guide Alias Electronics ERROR 280 ERROR 282 ERROR 283 ERROR 284 ERROR 290 EEPROM write error EEPROM error in settings EEPROM error in adjustments EEPROM error in log counter Error occurred during initialization the Alias cannot start 57 0060 284 12 APPENDIX D User guide Alias List of accessories and spares The standard shipkit for the Alias contains a shipkit list a silicon drain hose shipkit for the injection valve Valco Rheodyne or other 2 x 2 5A TL 250V fuse for power connection Wash bottle CD with user manual Needle for high titre plates needle for low titre plates is in instrument IO cable serial cable The following accessories are available for the Alias autosampler Vial adapter 2 mL vials Vial adapter 10 mL vials V
18. eagent B 1E3 However if you were to use a 12 vial tray for the Reagent you could use reagent from the same vial a number of times For example first line first sample position 2A1 last sample position 2A1 destination position 2E1 Reagent A 1A1 and Reagent B 1E1 Second line first sample position 2A2 last sample position 2A2 destination position 2E2 Reagent A 1A1 and Reagent B 1E1 third line first sample position 2A3 last sample position 2A3 destination position 2E3 Reagent A 1A1 Reagent B 1E1 etc If you chose to process plates in columns the following positions are available for sample destination reagent A and reagent B OOOOOOOO OOOOOOOO OOOOOOOO OOOOOOOO OOOOOOOO OOOOOOOO 25 eeeeeeen eeeeeeee eeeeeeee eeeeeeen eeeeeeee eeeeeeee e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e Sample e Destination Q Reagent A e Reagent B 0060 284 12 User guide Alias For example you can now program the following two 96 low plates processed in columns first line first sample position 2A last sample position 2A1 destination position 2A7 Reagent A 1A1 and Reagent B 1A7 second line first sample position 2B1 last sample position 2B1 destination position 2B7 Reagent A 1B1 and Reagent B 1B7 thir
19. er 3 twice 5 Rinse buffer tubing and needle with wash solvent The mix is performed from the destination position when the previous ADD action is TO DESTINATION When the previous ADD action is TO SAMPLE the mix is performed from the sample position 23 0060 284 12 User guide Alias Wait the programmed period of time before continuing with the next step reaction time A maximum of 15 steps can be created for the Mix amp Dilute routine Sample positions Mix amp Dilute If a mix is programmed the reagent solvents destination and sample positions in the trays are as follows Q Reagent A Left plate Reagent B Left plate Samples Right plate Destination Right plate If you chose to process plates in rows the following positions are available for sample destination reagent A and reagent B eee V sampe ee e e e Destination OOOO0eeee OoOOoOO0O0eeee ee e e 24 0060 284 12 For example you can program the following two 96 low plates processed in rows User guide Alias first line first sample position 2A1 last sample position 2A1 destination position 2E1 Reagent A 1A1 and Reagent B 1E1 Second line first sample position 2A2 last sample position 2A2 destination position 2E2 Reagent A 1A2 and Reagent B 1E2 third line first sample position 2A3 last sample position 2A3 destination position 2E3 Reagent A 1A3 R
20. er guide Alias Loosen the lower socket head screw number 2 Loosen the upper socket head screw number 1 Remove the syringe pull out the top of the syringe first Remove the syringe valve and install a new one Install the new syringe o0 Ff Q I Fasten the two socket head screws again fingertight 1 4 turn Fuses Fuses of the following types are installed in the Alias 2x2 5A Disconnect the Alias from its power source if you need to replace fuses If you need to replace the fuses make sure that you install fuses of the same type and rating Fuses are in the fusebox at the back of the autosampler Contact Service if problems with fuses are recurring 37 0060 284 12 User guide Alias CHAPTER 4 Trouble shooting Even though great care was taken in the design of the Alias problems may occur instrument errors on page 38 these can be caused by a variety of reasons software errors on page 39 usually caused by faulty communication between instruments or by faulty installation of the software analytical problems see Analytical trouble shooting on page 40 these may occur e g as a result of wear of parts errors in injection settings and methods or a wrong combination of sample loop buffer tubing and syringe Alias Service Manager contains a Service option select Alias Service Note that an access code is required for this option and that the service option is intended for ser
21. esigned for Large Sample Volumes LSV Sampling Sampling capacity 24 vials of 10 mL LSV Vial dimensions cap included Maximum vial height 47 mm Minimum vial height 32 mm Loop volume Not programmable injection volume determines the aspirated sample volume Dispenser syringe 2500 uL syringe Injection volume 0 uL 19 999 uL with 1 uL increments Valve Valco 0 75 bore valve Sample loop 10 mL SS sample loop 1 8 tubing with 1 16 tubing ends and fittings Valco Buffer tubing 2 mL Needle LSV needle with LSV air needle Promis and seal 52 0060 284 12 Analytical performance Injection method Reproducibility Viscosity range Memory effect 53 User guide Alias Partial loopfill injection mode RSD lt 1 0 for partial loopfill injections injection volumes gt 10 uL up to 50 of the installed sample loop 0 1 5cP lt 0 1 with programmable needle wash 0060 284 12 User guide Alias APPENDIX B Control I O connections The Alias has two I O connections RS232 connector for serial communication using the SparkLink protocol Contact closures output and TTL inputs connector The manufacturer will not accept any liability for damages directly or indirectly caused by connecting this machine to instruments which do not meet relevant safety standard WARNING The IO connector contains active high or active low TTL inputs and one contact closure output user definable in the System Settings
22. h a 500 uL syringe but a 2500 uL syringe can also be installed for the Prep version Figure 12 Syringe VU T 34 0060 284 12 tip User guide Alias Execute the following steps to install a different syringe 9 In Direct Control click Exchange in the Syringe group box Unscrew the syringe from syringe valve but make sure that the connector in the valve remains in place Disconnect the plunger from the syringe drive Fill the new syringe with wash solvent preferably IPA isopropanol Make sure that most air bubbles are removed from the syringe Connect the plunger of the filled syringe to the syringe drive and connect the syringe with the connector at the syringe valve Screw the syringe firmly into the connector In Direct control click Home in Syringe group box The syringe moves to home position and its content will be dispensed to syringe waste If there is still some air in the syringe click End again in Direct control The syringe is filled with wash solvent Use IPA Click Home again to dispense the wash solvent to waste If there is still air in the syringe repeat steps 8 and 9 and gently tap the syringe as the wash solvent is dispensed to syringe waste Perform a standard wash routine Direct control click Start Stop in de Initial wash group box All tubing connected to the syringe valve will be refilled and flushed Syringe plunger amp plunger Execu
23. he back of the autosampler and a cooling cover is installed inside the sampling compartment Prep version Alias suitable for large volume sampling Because larger volumes must be injected for the Prep mode see Specifications Prep version on page 52 Alias is fitted with a 2500 uL syringe and a 10000 uL sample loop The following user installable options are available Bio compatible sample flow path and valve Prep Kit Air needles Valve unit Inert sample needle Silco steel and bio compatible valve PEEK 2 5 mL syringe Prep valve 10 mL sample loop LSV needle and sample tray for 10 mL vials 6 different types of air needles are available for the Alias each for a different type of well vial plate However it is not just the type of well vial plate that determines which air needle must be used Refer to the section on Air needles see Air needles for Alias on page 31 for more information Special valve unit that cab be replaced quickly and easily 0060 284 12 User guide Alias Injection principles Figure 10 PASA injection concept Three injection modes can be used Full loop injections on page 14 for maximum precision Partial loopfill injections on page 17 for maximum flexibility JL Pickup injections on page 19 for zero sample loss These three injection modes accommodate use of a wide variety of applications For all injection modes loop injection with Pressure Assisted
24. he danger sign warns about a hazard It calls attention to a procedure or practice which if not adhered to could result in injury or loss of life Do not proceed beyond a danger sign until the indicated conditions are fully understood and met The warning sign denotes a hazard It calls attention to a procedure or practice which if not adhered to could result in severe injury or damage or destruction of parts or all of the equipment Do not proceed beyond a warning sign until the indicated conditions are fully understood and met The caution sign denotes a hazard It calls attention to a procedure or practice which if not adhered to could result in damage or destruction of parts or all of the equipment Do not proceed beyond a cautions sign until the indicated conditions are fully understood and met The attention sign signals relevant information Read this information as it might be helpful The note sign signals additional information It provides advice or a suggestion that may support you in using the equipment 0060 284 12 User guide Alias Safety practices The following safety practices will ensure safe operation of the autosampler and should only be executed by authorized personnel A Removal of some panels exposes potentially dangerous voltages Disconnect the instrument from all power sources before removing protective panels Replace blown fuses with size and rating indicated on the fuse panel or holder and
25. ing ensures consistent results Read this chapter to help identify parts of the Alias autosampler and to learn more about injection principles 0060 284 12 User guide Alias Instrument description Figure 1 Open the door Figure 2 Push the door upward The Alias is a complete autosampler that requires very little bench space Standard high or low well plates or vial trays can be used The sampling compartment of the Alias can house two different well plates Any combination of well plates is allowed except for 384 Low on the left and 96 High on the right The Alias autosampler is standard fitted with 15 uL injection needle 500 uL syringe 1000 uL buffer tubing 100 uL sample loop All replaceable parts are easily accessible Refer to the List of accessories and spares see List of accessories and spares on page 58 for more information To open the door execute the following steps 1 Get hold of the door handle 2 Gently pull it towards you and push it upward until it is in horizontal position 0060 284 12 Figure 3 Slide the door into the autosampler Figure 4 Location of Black push buttons User guide Alias Slide the door into the autosampler To remove the cover of the Alias 1 Press the two black buttons on either side top of the autosampler simultaneously User guide Alias 0060 284 12 2 Gently pull the cover towards you
26. items have been installed the following fluid connections are in place Figure 14 Alias fluid MR I connections Jump BOTTLE CAI INECT position War 04D position The Alias is factory installed with 500 uL syringe 100 uL sample loop 1000 uL buffer tubing 15 uL stainless steel sample needle Make sure that the following are correctly connected HPLC pump to port 1 of the injection valve HPLC column to port 6 of the injection valve Alias tubing The Alias is standard fitted with the following tubing Tubing Materials Dimensions Standard sample needle and SS 97 mm x 0 8 mm OD x 0 25 mm ID tubing label 15 uL ETFE Tefzel 200 mm x 1 16 OD x 0 25 mm ID Buffer tubing from high ETFE Tefzel 1275 mm x 1 16 OD x 1 0 pressure valve to syringe valve mm ID label 1000 uL 46 0060 284 12 Figure 15 Tubing guide Figure 16 Top view of tubing guide User guide Alias Tubing syringe valve to wash PTFE 400 mm x 1 8 OD x 1 6 mm ID solvent bottle Tubing syringe valve to waste PTFE 400 mm x 1 8 OD x 1 6 mm ID Note the following if you need to install new tubing insert tube ends always flush with ferrule ends do not overtighten nuts as this may cause blockage in the flow path make sure that you always use tubing volumes that are suitable for use with the other items in the flow path Tubing guide To prevent that the wash tubing obstructs the horizontal movement of the nee
27. led wash solvent bottle Open Direct Control in Alias Service Manager In the Syringe group box click End A syringe volume of wash solvent is aspirated from the wash solvent bottle and the wash solvent tubing is filled Click Home The syringe contents is dispensed to syringe waste Repeat steps 3 and 4 until the wash solvent tubing and the syringe are completely filled When wash solvent tubing and syringe are completely filled click Start in the Initial wash group box to perform a standard wash routine All tubing connected to the syringe valve will be rinsed with wash solvent Click Close to leave the Direct control screen The Alias is initialized 48 0060 284 12 User guide Alias Syringe A 500 uL syringe is standard installed in the Alias However it is also possible to install a 2500 uL syringe Refer to Syringe dispenser on page 34 for more information on how to replace the syringe Note that the Alias will give the best results if all air is removed from the syringe Execute an extra wash to remove air from the syringe Sample handling Take the following into account when handling samples Standard vials can best be filled by means of a narrow end pipette to allow air to escape when filling the vial Do not fill vials wells to the edge If you do sample will be forced into the air needle risking cross contamination of samples and soiling the needles tis important that seals and capmats are air
28. lute routine can be created for the Alias This routine allows you to process the sample before injection You can program three different types of actions Add the indicated volume from the Sample Reagent A Reagent B Wash position and dispense it to the Sample Destination position To prevent cross contamination the Alias will aspirate an additional volume of 25 of the programmed volume to flush the tubing and needle The aspirate and dispense speed depends on the selected syringe and the programmed syringe speed Example ADD 200 uL from Reagent A to Destination will result in the following actions 1 Aspirate an air segment of 5 uL to separate the wash solvent in the buffer tubing from Reagent A 2 Aspirate 50 uL Reagent A to flush the tubing and needle 3 Empty the syringe to the syringe waste position Aspirate 200 uL Reagent A and dispense it to the destination vial 5 Rinse buffer tubing and needle with wash solvent Mix aspirate and dispense a number of the times the programmed volume from the destination vial If no destination vial is available the mix is performed in the sample vial Example Mix 3 times with 250 uL will result in the following actions 1 Aspirate an air segment of 50 uL to separate the wash solvent in the buffer tubing from the solvent to be mixed Empty the syringe to the syringe waste position 3 Aspirate 250 uL solvent and dispense it back into the vial well Repeat step numb
29. nance 27 Mix amp dilute 23 59 User guide Alias O Options 11 P Partial loopfill injections 12 17 Pictorials used in this manual 2 R Replacing the sample needle 29 33 Replacing the Syringe dispenser valve 36 S Safety practices 3 Sample handling 49 Sample loop 29 Sample positions Mix amp Dilute 24 Software errors 38 39 Specifications 50 Specifications Prep version 11 13 52 Syringe 49 Syringe and buffer tubing 13 29 Syringe dispenser 34 49 Syringe plunger amp plunger tip 35 T Trouble shooting 38 U Upload notification 45 W Wash solvent and syringe rinse 48 Waste tubing 48
30. needle with capmat 56 mm air needle Example You have a Greiner deep well with Micronic capmat M53000 the Alias has a standard needle height setting Calculations will be as follows Ht 2 41 4 mm The following is true Dw 37 8 mm 41 4 37 8 2 3 6 is between 2 and 6 mm Cd 3 8 mm Protrusion length 41 4 3 8 6 0 2 0 29 6 Nh 6 0 mm standard Ac 2 0 mm minimum An air needle of 56 mm is required Air needle replacement Execute the following steps to replace the air needle 1 Remove see Replacing the sample needle on page 29 the sample needle Unscrew the chrome locking nut to remove the air needle Unscrew the chrome locking nut from the adjustment nut 33 0060 284 12 User guide Alias Get the new air needle Screw the height adjustment nut to the chrome locking nut thread of the height adjustment nut must be level with the lower part of the locking nut Make sure the O ring seal is in the locking nut 6 Install the air needle Install the sample needle Program the proper needle height for the new needle in the ASM settings window Go to Adjustments to adapt Needle Tray settings if necessary If you use trays with 12 vials or 48 vials make sure that the needle height settings is 2mm to prevent the WARNING needle from touching the bottom of the vials 9 Doaninitial wash from Direct control to rinse the needle Syringe dispenser The Alias is standard supplied wit
31. o SBS standards 96 well high low and 384 well low formats 48 vial or 12 vial trays any combination of plates is allowed except for 384 Low left and 96 High right Max plate vial height 47 mm incl septa or capmat 1 5000 uL programmable 10 mL loop optional 500 uL standard or 2500 uL for Prep option Missing vial well plate detection by sensor Built in compressor but only for vials with septa Electrically 100 msec 0060 284 12 Piercing precision needle Wash solvent Wetted parts in flow path Injection cycle time Analytical performance Injection modes Reproducibility valid at 1 0 cP Memory effect Programming Interface Injection methods Injection volume Max injection volume Injections per vial well Analysis time Wash Timed events Priority sample Communication Outputs Inputs Serial communication port 51 User guide Alias 0 6mm Integrated wash solvent bottle SS316 PTFE TEFZEL VESPEL Glass Teflon Optional PEEK lt 60 sec in all injection modes for 1 injection lt 100 uL including 300 uL wash Full loop partial loopfill and uL pickup mode PASA pressure assisted sample aspiration RSD lt 0 3 for full loop injections RSD lt 0 5 for partial loopfill injections injection volumes gt 10 uL RSD lt 1 0 for uL pickup injections injection volumes gt 10 uL lt 0 05 with programmable needle wash via Alias Service Manager
32. o reset the system and prepare it for normal use Execute the following steps if you are asked to switch the system off and then on again 1 Check that the communication cable between Alias and PC is properly installed 2 Turn the instrument off with the on off switch at the back of the autosampler 3 Turn the system on again with the on off switch The system is initialized and is now ready for use Software errors Software errors usually are caused by faulty installation of the software or by faulty communication between instruments you will be asked to re install the software on the PC that controls the system If a software error message appears first check if it may be caused by faulty communication between instruments Check all cable connections between instruments Open Alias Service Manager Select Alias Direct Control Click Initialize A O N 39 0060 284 12 User guide Alias Analytical trouble shooting Analytical problems like bad reproducibility or carry over may occur in any HPLC system It may be hard to find the cause you may have to try out several procedures The first thing to do is to determine whether the problem is caused by the autosampler or by the rest of the system 1 Replace the valve by a manual injection valve to discriminate between valve problems and other problems 2 Do a number of Full loop injections If the results are fine the fault is in the autosampler if not check
33. olume loop volume 3 x needle volume 2 Full loop gives maximum possible reproducibility 0 346 but not maximum accuracy since the loop volume is specified with an accuracy of 10 Minimum sample loss 230 uL 2 x loop overfill flush volume for 15 uL needle Partial loopfill gives maximum accuracy plus reproducibility better than 0 5 RSD for injection volumes gt 10 uL Minimum sample loss Flush volume 30 uL 30 uL is the recommended minimum flush volume smaller flush volumes can be programmed but will result in decreasing performance uL Pick up offers no sample loss maximum accuracy same as partial loopfill but slightly lower reproducibility RSD better than 1 for injection volumes gt 10 uL User guide Alias 0060 284 12 Full loop injections The sample loop is completely filled quantitatively with sample This type of injection results in extremely good reproducibility The initial situation the injection valve is in INJECT position The sample needle with air needle has entered the well or vial Headspace pressure applied through the air needle ensures that no air or vapor bubbles are formed during sample aspiration 1 gt Column a Syringe Sample 2 The syringe dispenser aspirates the flush volume from the sample well vial to fill the sample line with sample and remove wash solvent Column c a Syinge S
34. pply systems for equipment with rated current lt 16A Use shielded cables and connectors for all remote connections Emmen February 2006 Rein van den Berg QA manager 0060 284 12 User guide Alias Contents About this guide 1 Pictorials used in this manual ineei eeii R E R E R REEE 2 Satety practiCes cuoc tg ue aaea e a aa n sass E HP a ee a aa chee EEE e N tenet 3 Introduction to Alias 4 Instrument description 2 o eee Uu E ese Pe E E E 5 Se D 11 Hausen e diene 12 Mix Se dilute iter e e et e EDO REO EH te P RED MEE ir RE Eaa 23 Maintenance 27 leui 27 Injection valve and rotor seal ete tira tere be hir tie RR hene 28 Sarmple loop 54 e o ege o ID nO Dpme npe ine aquitib aci ater 29 Replacing the sample ncedle 3 5 3 ites mino os Bae idtm ae eletto batteries amides 29 Air needles for ATIaS 4 co re te ee tates Such Sine ele d Regni Led e es tpe et Dv ir ee tenes 31 SYTIN GS dispenser cn eto oer utei ad die e Re pta or e etr ER eco 34 Syringe plunger amp plutigertip 3 ue oe eo pete nb aep eniti be ake 35 Replacing the Syringe dispenser valve sessi enne nennen enne nre nenne nnne 36 ll MEME 37 Trouble shooting 38 Instr ment errors 3 58 E E E ERREUR RESTER E ERAI E EEEE G 38 SoftWare STLOMS 3 urere E E TE EE Ee EERE p Ae E AEEA A E T qn MUSEI EE r E 39 Analytical trouble shooting 2 So e t eb tr peri ene aerei i ie e ded
35. proper combination of syringe and tubing see Syringe and buffer tubing on page 13 to ensure good results Take the following into account when you have installed a sample loop connect the loop between ports 2 and 5 of the injection valve go to the configuration settings and adapt settings in the flowpath group box if you have installed a loop with a different volume Remember that the maximum injection volumes are calculated with the following formulas full loop injection volume loop volume partial loopfill maximum injection volume 50 of the loop volume uL pickup maximum injection volume loop volume 3x needle volume 2 Replacing the sample needle Execute the following steps to replace the sample needle Open Direct control Alias Service Manager Click Exchange in the Needle group box The needle moves to exchange position Loosen the needle connection nut number 3 Loosen the nut number 1 that connects the tubing number 2 to port 4 of the injection valve Remove the sample needle by pulling it out of its fitting by the tubing Install a new needle assembly make sure that the air seal is around the needle Tighten the needle assembly with the needle connection nut Connect the other end of the needle connection tubing to port 4 of the injection valve Do not tighten too much as this may block the tubing Click Initialize in Direct control The sample needle moves back to home po
36. s for initial installation of the Alias autosampler 1 Liftthe Alias from its packaging using both hands at the marked position 2 With both hands under the instrument lift the Alias to its operating location Keep the instrument upright 43 0060 284 12 User guide Alias Make sure that the ventilation holes at the back of the autosampler are not blocked Note that if the ventilation holes are blocked this may influence performance and cooling capabilities of the autosampler WARNING If objects are placed on top of the Alias this may also influence the cooling capabilities Objects can be placed on any side of the Alias however make sure these objects are placed at a distance of 5cm from the Alias if objects are placed at only one side of the Alias 10cm from the Alias if objects are placed on more than one side of the Alias Do not place the Alias in an area subject to excessive dust or shocks Use the Alias indoors only Do not place it near a source of heat or in direct sunlight as this may influence the cooling capabilities of the system Leave the Alias to adopt ambient temperature for at least one hour Install Alias Service Manager see ASM software on page 45 on your PC Check that fuses and voltage range on the rear side of the instrument match that for the power outlet to be used Connect the Alias to the PC COM port with the cable provided with the Alias Connect the power c
37. sition 29 0060 284 12 User guide Alias 10 Perform a wash routine to clean the new needle by clicking Start in the Initial wash group box of Direct Control Click Stop to end the wash routine 11 Use the Alias Adjustments option to adapt Needle Tray settings If you use trays with 12 vials or 48 vials make sure that the needle height settings is gt 2mm to prevent the needle from touching the bottom of the vials WARNING Figure 11 Replacing the sample needle 30 0060 284 12 User guide Alias Air needles for Alias Six types of air needles are available for the Alias autosampler all different in length difference of 6 mm These air needles are required to accommodate use of different plate heights in the Alias For every well vial plate the correct air needle is available Apart from the 6 mm difference in length between the air needle types the needle holder allows for an extra 6 mm variation in needle height Standard air Needle The standard air needle is a 62 mm needle no 0045 505 This air needle accommodates use of a wide range of high and low plates See the illustrations below for the puncturing depth of the needle hii 10 mL vial 2 mL vial Greiner deep well with capmat Greiner low well 31 0060 284 12 User guide Alias Note that no PASA should be used for low wells as the sample needle sufficiently punctures the seal to prevent vacuum the function of the air needle will
38. software Full loop partial loopfill and uL pickup 0 uL 9 999 uL with 1 uL increment depending on system settings Full loop loop volume Partial loopfill 12 x of loop volume uL Pick up loop volume 3 x needle volume 2 max 9 injections max 9 hr 59 min 59 sec Programmable Wash between injections and Wash between vials Programmable 4 x AUX ON OFF Programmable 1 programmable relay output programmable as Inject marker default Auxiliary Alarm 2 programmable TTL inputs programmable as Next injection input default Freeze input Stop input RS232C standard 0060 284 12 User guide Alias Options factory installed Sample tray cooling Built in Peltier cooling Range 4 C to Ambient 3 C Temp air temperature in sample compartment 4 C 2 C at temperature sensor Temperature at relative humidity of 80 and ambient temperature of 25 C Communication hardware On request RS422 485 CAN Ethernet Options user installable Bio compatible sample flow Inert sample needle Silco steel and bio path and valve compatible valve PEEK Prep Kit see specifications Prep 2 5 mL syringe Prep valve 10 mL sample version see Specifications loop LSV needle and sample tray for 10 Prep version on page 52 mL vials Specifications Prep version Note that this specification only lists items that are different from the standard Alias specification The Prep version of Alias is d
39. t with Partial loopfill injections An air segment can be used to reduce the amount of flush volume The air segment is at the front of the flush volume and will not be injected With a standard needle the flush volumes must be a minimum of 30 uL for injections with air segment and 35 uL for injections without air segment If the samples are highly viscous it may be necessary to program larger flush volumes and reduce the syringe speed for better performance Needle njection loop Buffer tubing A Sample Eluent Flush Air C KALLE LA Epi l l l l B Sample l Eluent Flush t T DAAA Y N uL Pickup injections The switching sequence for uL pickup injections is 1 Inthe initial situation the injection valve is in INJECT position The sample needle has entered the transport position a Syringe gt Sample Transport liquid 0060 284 12 User guide Alias 2 For the first injection after a wash or after emptying of the buffer tubing the syringe dispenser aspirates a transport plug from the transport position to fill the sample line with transport liquid and remove wash solvents a lt Syringe Z Column gt Transport liquid 3 The needle moves from the transport position to the sample vial The injection valve switches to LOAD position lt a Syinge amp Column gt Sample
40. te the following steps to replace the plunger or plunger tip ON Oa FW DN In Direct control click Exchange in the Syringe group box Remove the syringe Slide the plunger out of the glass part of the syringe With pliers remove the tip Dampen the new tip with for example isopropanol Mount the new tip on the plunger Insert the plunger in the glass part the syringe Install the syringe in the autosampler again 35 0060 284 12 User guide Alias Replacing the Syringe dispenser valve Figure 13 Replacing the syringe dispenser valve The syringe valve is a 4 port selection valve Ports are assigned as follows Waste Use this port as a drain for the syringe dispenser Wash Use this port to aspirate wash liquid from the wash bottle or in case of multiple wash liquids connect it to the solvent selection valve Needle Connect the buffer tubing to this port All connections to the syringe valve must be made using fingertight fittings An exception can be made for the waste outlet the port on the rear of the valve Execute the following steps to replace the syringe dispenser valve Place the syringe valve in waste position before you replace the syringe valve In this position the mounting screws are opposite in line with the holes 1 N x I L Je i al re o f 9 Ac A Move the syringe to exchange position use the Exchange button in Direct control 0060 284 12 Us
41. th the injection valve You can clean this bin with a damp cloth with non aggressive cleaning liquid sample tray if sample has been spilled on the sample tray clean the tray with a damp cloth with non aggressive cleaning liquid drain tubing regularly flush the drain tubing with solvent to prevent clogging and to ensure that liquids and condensate are disposed of 27 0060 284 12 User guide Alias Injection valve and rotor seal The Alias is equipped with an injection valve either with quick connect mounting or with fixed mounting Execute the following steps to remove the injection valve 1 A o ON O0 Oi Disconnect all tubing from the valve Only the sample loop can stay in place Remove the valve Remove the screws from the stator part of the valve Gently open the valve and take out the rotor seal Clean and or replace the seal Place the stator back on the rotor and fasten the screws Hold the valve for mounting with port 1 pointing upward Place the valve into its slot and fasten it Reconnect all tubing to the valve In Direct control click Initialize to make sure that the valve is in Inject position 10 Perform a standard wash Direct control Initial wash group box The Alias is now ready for use 28 0060 284 12 User guide Alias Sample loop The Alias is standard fitted with a 100 uL sample loop A different sample loop size can be installed but note that you will need the
42. the rest of the HPLC system Please bear in mind that analytical problems may also be caused by external influences like temperature or light sensitive samples Make sure that the application was running trouble free before and that no changes have been made to the system A number of causes and possible solutions for analytical problems is listed below Contact service if you need further help If reproducibility is not according to specifications check the following possible causes Causes Solutions Air in flow path Do an initial wash select Alias Direct Control in Alias Service Manager Leaking syringe If leakage occurs at the top of the syringe check whether it has been properly mounted If leakage occurs at the bottom of the syringe replace plunger tip or syringe Leaking syringe valve Check or replace valve Rotor seal worn out Replace seal Check stator Dead volumes in tubing Redo connections with new ferrules and nuts connections If a blank gives a peak that is too high for your criteria Causes Solutions Solubility problem You can either modify your sample or accept carry over 40 0060 284 12 Bad match between sample characteristics and hardware The blank you use has been soiled Cause not clear 41 User guide Alias Check hardware Needle either use an extra wash to wash the inside and outside needle or install a different type of needle Steel or Silica coated Val
43. tight to prevent air bubbles from forming and to block evaporation of volatile samples We recommend use of the following seal types for standard low well plates sealing tape for deep well plates pierceable capmats Pre slit or silicon or sealing tape for vials standard septa thin types do not use vials with hard caps that are not designed for being pierced by an injection needle do not use e g Eppendorf SafeLock micro test tubes When you use uncapped vials wells injection performance may not be to specification 49 0060 284 12 APPENDIX A Specifications General Sound pressure level Working temperature Storage temperature Humidity Safety and EMC compatibility Installation class Pollution degree Altitude Dimensions Weight Max weight that can be placed on top of Alias Power requirements Viscosity range Sampling Sample capacity Vial Plate dimensions incl cap Loop volume Dispenser syringe Vial detection Headspace pressure Switching time injection valve 50 User guide Alias LeAq 70 dB 10 40 C indoor use only 25 60 C 20 80 RH According to EC directives CSA UL approved II 2 up to 2000 m 300 mm x 510 mm x 360 mm without cooling option 300 mm x 575 mm x 360 mm with cooling option 19 kg without cooling 21 kg with cooling 65 kg 95 240 Volt AC 10 50 60 Hz 200VA 0 1 5 cP 2 Micro Titre Plates according t
44. ve replace rotor in valve by Valco E or H type Tubing install different tubing Steel Peek between autosampler and column or use different wash solvents Use a new blank Check if you can solve the problem by using more variation in solvents 0060 284 12 If no injection takes place Causes Blockage in flow path Leakage in the injection valve CAUTION 42 User guide Alias Solutions 1 2 3 o0 5 W wm Disconnect needle from valve Start a manual wash If solvent flows from the injection port check the needle if no solvent flows from the injection port disconnect buffer tubing from valve Start a manual wash If solvent flows from open end check rotor seal if not disconnect buffer tubing from syringe valve Start a manual wash If solvent flows from syringe valve check buffer tubing if not check for over tightened connections in the entire flow path and check the syringe valve Disconnect the needle tubing and buffer tubing Connect port 1 to an HPLC pump Block port 6 Start the pump at a low flow Observe ports 3 and 4 for leakage If leakage occurs at ports 3 and 4 check rotor seal if not recheck with manual valve Observe the maximum allowed pressure of 350 bar to prevent leakage in the valve 0060 284 12 User guide Alias CHAPTER 5 Installation Check that all items refer to packing list are present Alias autosampler Execute the following step
45. vice engineers only Contact your supplier if a problem occurs that you cannot solve Instrument errors Incidental fault conditions may occur in any instrument The Alias will generate an instrument error message with an error number a short description of the error and instructions on how to proceed In most cases you will be asked to either initialize the system or to switch the system off and then on again Always click OK and follow the instructions to resolve the error status Use Alias Direct control in Alias Service Manager to monitor the error Initialize the system in the Alias Direct control window Make sure Alias is connected to a grounded power source WARNING Ifthe LED is not lighted a fuse may have blown Checking a valve implies that you remove the valve and check all parts for wear and dirt Execute the following steps after any problem with a valve has been resolved 1 Select Alias Direct control The Direct control window appears 2 Click Initialize 38 0060 284 12 User guide Alias 3 Inthe Initial wash group box click Start to start the wash 4 Click Stop to end the wash 5 Click Close to exit the Direct control window Execute the following steps if you are asked to initialize the system 1 Select Alias Direct control The Direct control window appears From this window you can control separate parts of the autosampler to check whether they function as intended 2 Click Initialize t
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