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Maintainor® Tissue, Instructions for use

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1. foil adhesive Tissue User Manual Product description The Maintainor Tissue is designed for use with Stabilizor T1 instrument to quickly stop ex vivo degradation of proteins in tissue samples Rapid inactivation stops all enzymatic protein degradation processes and heat stabilizes the sample for further analysis Fresh or frozen samples up to 7 mm thick can be treated in the Maintainor Tissue card Intended use For research use only Maintainor Tissue is designed for single use only Dispose of used Maintainor Tissue card in accordance with local regulations Storage Un used 10 to 50 C Containing sample 20 to 80 C Do not place the card in liquid nitrogen Main parts MARKING AREA e SAMPLE CHAMBER 33x7 mm e AIR SEAL e _ _ VALVE e o al COVER LID e Ordering Information Product Pack size Order number Maintainor Tissue 12 cards DMT0001 Maintainor Tissue User manual DPM0009 rev 3 2012 Stabilizor and Maintainor are regisered trademarks of Denator AB AN denalor Denator AB Arvid Wallgrens Backe 20 SE 413 46 Gothenburg Sweden e mail order denator com web www denator com
2. 1 Prepare amp set up Stabilizor T1 In order to minimize the ex vivo time on tissue samples make sure that everything is ready for heat stabilization prior to sample excision Choose the appropriate program for your application or prefered custom settings see Stabilizor T1 User manual Samples can be heat stabilized from both fresh and frozen state 2 Prepare Maintainor card o Mark Maintainor card with sample ID and additional information or o Mark Maintainor Card with a custom barcode label Please check temperature spec for barcode print Open the cover lid once fully to enable easy access when mounting sample 3 Mount sample o For fresh samples place the opened Maintainor card in the Stabilizor instrument for direct heat stabilization o For frozen samples place the opened Maintainor Card on ice m ail did a x K o Place one 1 sample in the chamber center 7 wet xt oe Close the cover gently with a swirling movement of your fingers 4 Heat stabilize sample Place the Maintainor card immediately in Stabilizor instrument for stabilization or store frozen until heat stabilized Be careful not to damage the cover foil If the cover is damaged air leakage can result in failed heat stabilization Do not put the Maintainor card in liquid nitrogen The Maintainor card can withstand temperature down to 80C Do not use high concentration organic solvents as these can damage the

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