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USER MANUAL - Loligo Systems

1. On Screen 5 the product information of the products to be installed are shown Screen 5 Jil AutoResp 4 Lica bal Product Information Please read the following information about the products to be installed NI DAQ 8 5 Device Support MI DAQme 6 5 supports the latest DAQ devices including C Senes M Seres E Seres 5 Seres B Seres USB Seles Analog Output avelomm Counter Timer Digital 1 0 Nl 653s D54 Ga and SCC devices The last version of NI DAQms to support the PLI 7047 6040E device is 8 7 if you need support for this device do not install thie version of Nl DAlme Refer to the NI DAQ Readme for supported device models Traditional NI DAQ Legacy is an older diver with an outdated API that supports some additional alder DAQ devices but not all of the latest DAQ devices Applications developed with earlier versions of NI DAQ wll run under Traditional NI DAQ Legacy ou can use both NI DAQms and Traditional NI DAQ Legacy in the same computer but must reset devices before changing from Traditional NI DU Legacy to Nl DAGmes Refer to the NI DAQ Readme for guidelines Support Removed from NI DAQ 7 0 and later NI DAQ 7 0 and later supports differant devices operating systems application software and programming language versions than previous releases of NI DAG Starting with version 7 0 NI DAGQ device support excludes Val DAC Remote SCH 1200 Seres DAG the DAQCard 500 700 family parallel port DAGP ads and several ot
2. 1100 00 10 34 45 10 35 45 Page 27 of 55 L oLico LOLIGOSYSTEMS COM 5 2 2 Sample frequency button Use this button to set a common sample frequency for all inputs The frequency can be set from one sample to ten samples per second Please note All inputs are always sampled at the same rate 5 2 3 Barometic pressure hPa Use this button to set the days actual barometic pressure in hPa This field is used only in fiber optic measurements and is disabled enabled together with the fiber optic button status OFF ON The default value is 1013 1 atm 760 mmHg 5 2 4 pO2 sat kPa Use this button to set the saturated partial pressure of oxygen in kPa see 8 1 pO2 kPa table on page 50 This field is needed to convert the oxygen values in kPa to Yair sat and oxygen sat via the Unit button see 5 2 11 2 Unit button on page 34 The default value is 20 859 which corresponds to 1013 kPa at 15 ga 5 2 5 Fiber optic button Use this button to select the type of oxygen input When the button displays Fiber optic off chamber oxygen input should be 0 5 VDC e g from OXY REG instrument When the button displays Fiber optic on the chamber oxygen input is acquired via the PreSens fiber optic instrument and the RS232 port of the PC Pressing the button toggles Screen 27 and Screen 29 seen on page 35 Before proceeding to Fiber Optic on the user is prompted to choose the COM port the fiber optic instruments from PreSens
3. 19 413 19 464 19 516 19 568 19 620 19 672 EAZ EAA 19 827 19 879 19 930 ETETA 20 034 20 086 Page 50 of 55 LOLIGOSYSTEMS COM Oxygen solubility in mg O2 liter kPa at From Green amp Carrit 1967 J Mar Biol 25 140 147 1 kPa 7 501 mmHg different temperatures and salinities Salinity o o o tat el a of a tw wi 20 22 24 ze za aa 32 34 86 Temperature deg Ch Page 51 of 55 Constants 7 424 0 1288 273 16 John Fleng Steffensen 2002 4417 53 44 2 927 0 0444 0 04236 0 00071 8 2 Oxygen solubility L oLico LOLIGOSYSTEMS COM 8 3 DAQ 4 Instruction manual The DAQ 4 instrument is used for data acquisition and relay controlling It is designed to run with the software AutoResp 4 but it can be used with other applications LIST OF PARTS DAQ 4 instrument AutoResp 4 Power Cord USB cable 4 fold extension cord for pumps qty 2 User manual SETUP TO power up the instrument connect the power cord to the outlet 100 240 VAC 50 60Hz on the back side of the instrument Connect the USB cable to an USB port on your PC and to the outlet on the front side of the instrument named PC USING THE DAQ 4 FOR DATA ACQUISITION The DAQ 4 instrument acquires data on 6 channels and converts the analog signals to 16 bit values Connect the inputs to controller instruments e g OXY AM to an OXY REG with a data cable If you want to use controller instruments not from Lo
4. 19 56 101 05 21 033 21 013 20 991 20 965 20 937 20 904 20 868 20 827 20 781 20 730 20 672 20 499 20 274 19 986 19 850 19 62 101 32 21 088 21 069 21 046 21 021 20 992 20 959 20 923 20 882 20 836 20 784 20 727 20 553 20 328 20 039 19 902 19 67 AHB 21 144 21 124 21 102 21 076 21 047 21 015 20 978 20 937 20 891 20 639 20 782 20 607 20 381 20 092 19 955 19 72 101 85 21 199 21 180 21 157 21 131 21 102 21 070 21 033 20 992 20 946 20 894 20 836 20 661 20 435 20 144 20 007 19 77 P1022 21 255 21 235 21 212 21 187 21 158 21 125 21 088 21 047 21 000 20 948 20 891 20 715 20 488 20 197 20 059 19 82 102 39 21 310 21 290 21 268 21 242 21 213 21 180 21 143 21 102 21 055 21 003 20 945 20 770 20 542 20 250 20 112 19 87 102 65 21 366 21 346 21 323 21 297 21 268 21 235 21 198 21 157 21 110 21 058 21 000 20 824 20 595 20 303 20 164 19 93 102 92 21 421 21 401 21 378 21 353 21 323 21 290 21 253 21 212 21 165 21 113 21 054 20 678 20 649 20 355 20 216 19 96 Pp 103 19 21 477 21 457 21 434 21 408 21 379 21 346 21 308 21 267 21 220 21 167 21 109 20 932 20 702 20 408 20 269 20 03 po 203 45 21 532 21 512 21 489 21 463 21 434 21 401 21 363 21 321 21 275 21 222 21 163 20 986 20 756 20 461 20 321 20 08 J IO mn 18 895 18 947 18 999 19 050 19 102 19 154 19 206 EEE 19 309 19 361
5. Back side ANALOG OUT Analog oxygen output The output range is 0 5 VDC For using the OXY REG instrument in general the signal is available via pin 1 and pin 4 GND in the input connector REI Relay socket for 110 230VAC max 3A solenoid valve RE2 Relay socket for 110 230VAC max 3A solenoid valve POWER 24 230V UC Power input for the OXY REG instrument Connect the instrument to a grounded 24 230 VDC power supply using a standard pc type cable with a grounded wall plug For further information about the OXY REG instrument see 8 5 OXY REG manual page 55 Page 20 of 55 L oLico LOLIGOSYSTEMS COM 4 Introduction to intermittent flow respirometry Three different methods are commonly used for measuring oxygen consumption in water breathers e g fish e Closed respirometry e Flow through respirometry e Intermittent flow respirometry 4 1 Closed respirometry or constant volume respirometry Measurements are in a sealed chamber of known volume a closed respirometer The oxygen content of the water is measured initially t then the respirometer is closed and at the end of the experiment t the oxygen content is measured again Knowing the body weight of the animal the respirometer volume and the oxygen content of the water at time t and t the mass specific oxygen consumption rate can be calculated as follows VO Oa to O2 t1 V t BW VO oxygen consumption rate mg O2 kg hour O
6. Oo out oxygen content in water outflow mg O liter BW body weight of experimental animal kg The advantages of this method are several 1 the duration of the experiment is in principle unlimited 2 no accumulation of CO and other metabolites 3 its possible to measure at a constant oxygen level 4 by controlling the quality of the inflowing water it s possible to measure metabolism at different desired levels of oxygen salinity etc However this method bring along one significant disadvantage in order to determine oxygen consumption by open respirometry it is crucial that the system is in steady state This means that the oxygen content of the in flowing and out flowing water AND the oxygen consumption of the animal have to be constant If the oxygen consumption of the animal for some reason changes during the experiment steady state will not exist for a while Not until the system is in steady state again will the above formula give the correct oxygen consumption rate The duration of the time lag depends on the relationship between chamber volume and flow rate Thus open respirometry measurements have poor time resolution and are not suitable for determination of oxygen consumption on organisms with a highly variable respiration like fish Page 22 of 55 L oLico LOLIGOSYSTEMS COM 4 3 Intermittent flow respirometry or open closed respirometry Our systems for automatic respirometry works by intermittent flow respirometry
7. AutoResp 4 Page 10 of 55 LOLIGOSYSTEMS COM 13 Now the WiBu drivers will be installed When Screen 11 appears click Next LOLIGOSYSTEMS COM Screen 11 AutoResp 4 Installation Readme Please read the following Information WIBU KEY Setup ga Welcome to the WIBU KEY Software Setup program LW This program will install WIBU KE Y Software on your computer Ibis strongly recommended that you exit all Windows programe and the control panel before running this Setup program Click Cancele to quit Setup and then close any programs you have tunning ae i Click sMexte to continue with the Setup program Version 5 20b e Back Install Cancel 14 Select supported language and click Next 15 Select the options seen on Screen 12 and click Next Screen 12 AutoResp 4 Installation Readme Please read the following Information d WIBU KEY Setup Select the WIBLI KEY components you like to install e 5 Gi WENEEAWELAN Nanak Server rette TE he te tee T Install as NT service with autostart P WkNet Network Server for Novell Netware I WELAN Aa kNet Network Monitor 32 bit T wkNet Network Mentor 16 bit Additional WIBU KEY Tools Once you have selected the components you wish to install press the sNexts button to complete the installation lt Back Ven Cancel Page 11 of 55 16 Click Next again after you have accepted the summary of the installati
8. E 36 37 AMDIENE e EE 44 POU EE 37 ANNOG EE 4 Chamber EE 44 CheCkDOX E 40 41 Closed respirometr 21 CoO A T cecdteadacnconateniesnsconmenaeuaninennacouns 41 DAO EE 5 OY EE 17 46 Be Me nn 46 Riegel 25 Bike 8 13 EGE gt OXY TEE 45 Edit gt Temperature 45 Enable channel REENEN 36 Experiment settings EE 42 PID CE en Le 28 File gt Continue evperiment 41 SI LEE 41 File gt New experiment eee ees 26 File gt Stop experiment ccccseeeeeenees 45 Pie aC ar OTN 38 Fish respiration EE 21 Flow through respirometry 00cee ees 22 SEL EE 43 US 39 ISH DUD BEN 23 Gia errai 43 45 Help gt AD OUL EE 41 45 ALIDU se ssciennesestotnssenwonessterescen 31 32 33 PIV DOr e EE 5 40 EE eege 5 40 PLY SUCECS S eegene eege 40 41 ole EEN 30 31 32 33 EI tee 32 TUG Veto ee E 31 Intermittent flow respirometry 5 23 Ee E EE 4 EO Dal E 1 Sekt ei 30 31 33 Loop number EEN 43 L oLico LOLIGOSYSTEMS COM LOOP remaining cece cece eee e neces eeeee ee eaes 43 Measure period aa ENNER ENN 20 Measurement amp Automation 4 16 Metabolites NEEN ENN EEN 23 MO gt estiunsdasneusd tia tegsdeeanancuetsanasdannesnedon 44 MOV AVG WE 30 31 32 36 VE a 29 34 Note Ge BEE 20 Ee Ee TT e EE 20 GAN DEE 40 Open TESDIFOMCH EE 22 BA Ge E SA Oxygen partial pressure cceeee eee 21 27 OXYGEN regulation cccccceeeeeesseeeeeeeees A4 Reeg 27 Bit 37 KE 36 DO lower control cece cece eeeeee
9. NTI Backup NOW 4 EN NTI CH amp DVD Maker 7 E Philips SPC S00NC PC Camera ei Philips ToUcam Camera E Pinnacle ECH USB2 Completing the Found New Hardware Wizard The wizard has finished installing the software for Ke USB 6215 y Click Finish to close the wizard LoliResp Microsoft Access Microsoft Excel Microsoft FrontPage Ki Microsoft Outlook Microsoft PowerPoint Microsoft Word 4 Outlook Express Ke Remote Assistance Windows Media Player a Windows Movie Maker E QuickTime Sr Windows Live Messenger EN WIEL KE EN LoliTRACK E Canon Utilities E AutoResp4 E NI DAQ QS Measurement amp Automation NI Registration Wizard E DataSocket E VISA EA NI License Manager E REALTEK Gigabit and Fast Ethernet NIC Driver ei Startup E Vernier Software WinZip Cl Adobe Reader 7 0 S Internet Explorer Windows Home Edition QARAR TTY Sh zen LOLIGOSYSTEMS COM 28 Open Measurement amp Automation by following the folder hierarchy shown on Screen 21 Page 16 of 55 Screen 22 LOLIGOSYSTEMS COM OH NI USB 6215 Devi Measurement amp Automation Explorer File Edit View Tools Help Ou ration Al Properties X Delete Zb Self Test A Test Panels fJ ResetDevice ini Create Task d Configure TEDS EZ Hide Help EI Ex My System A a DN Data Neighborhood Name Value Back El E we Devices and Interfaces e serial Number OxE
10. cable For further information about the OXY 4 mini instrument see 8 6 OXY 4 Instruction manual page 55 Page 18 of 55 L oLico LOLIGOSYSTEMS COM 3 5 TMP REG instrument The TMP REG instrument is designed for monitoring and regulating water temperature in fish tanks respirometers etc 3 5 1 Front side IN Pt100 temperature input Connect the Pt100 temperature sensor to this input 3 5 2 Back side ANALOG OUT Analog temperature output The output range is 0 5 VDC For using the TMP REG instrument in general the signal is available via pin 1 and pin 4 GND in the input connector RE1 Relay socket for 110 230VAC max 3A pump cooling or heating coil RE2 Relay socket for 110 230VAC max 3A pump cooling or heating coil POWER 24 230V UC Power input for the TMP REG instrument Connect the instrument to a grounded 24 230 VDC power supply using a standard pc type cable with a grounded wall plug For further information about the TMP REG instrument see 8 4 TMP REG manual page 55 Page 19 of 55 L oLico LOLIGOSYSTEMS COM 3 6 OXY REG instrument The OXY REG instrument is designed for monitoring and regulating dissolved oxygen content of sea or fresh water in fish tanks respirometers aquaria etc 3 6 1 Front side IN Analog oxygen input The input range is 0 50 mVDC Connect the galvanic oxygen probe to this input O cal button Button for use for calibration of oxygen signal 3 6 2
11. eens 31 32 DO upper Control EEN 30 32 Bee 45 RAW data 29 38 Del Lee SE Relays are NOt workimg cece cece eeeeees 48 eebe 3 Sample frequency ccccceeeeeseeeeaeeees 28 SEELEN 40 41 Start EE 41 Steady Ee 22 EE EN Temp lower control cccceeeseeeeeeeeeees 33 Temp upper Control aa 33 Temperature E 44 Temperature regulation sssesssrerssrsrss 44 Time resolution cceceeeeeeeeeeeeaeeneees 22 23 TMP REG Ipmstrument 19 20 Troublesbootmg cece cece eee eeeeeeeeeeanee 46 WICW ee 45 Voltage INPut ccccee cece eee e eens 30 31 32 VOIUME saxeeaeesanedaeeunrt ai vsaneeondans cates anecas 40 Wait period ccccccceeeeeenssecseeeennnnnnees 39 Wet Ce LEE 40 Page 49 of 55 LOLIGOSYSTEMS COM 8 Appendix pO kPa table 8 1 File PO2 kPa xls Partial pressure of oxygen p02 at different barometric pressures and temperatures Temperature deg O OI 2 a ae ET EE ee ee ee ee Pvap kPa 0 61 0 71 0 81 0 93 1 07 1 23 1 40 1 60 1 82 2 06 2 34 3 17 4 24 5 62 6 28 7 38 Pop kPa Le 97 32 20 256 20 237 20 215 20 191 20 163 20 132 20 097 20 058 20 013 19 964 19 909 19 742 19 526 19 248 19 117 18 89 p97 59 20 312 20 292 20 271 20 246 20 218 20 187 20 152 20 113 20 068 20 019 19 963 19 796 19 579 19 301 19 169 18 94 97 85 20 367 20 348 20 326 20 302 20 274 20 242 20 207 20 167 20 123 20 073 20 018 19 850 19 633 19 353
12. is connected too Please note When fiber optic is on as seen on Screen 29 on page 35 the Sample frequency cannot be altered as it is predetermined by the fiber optic instrument from PreSens The frequency is approx 1 Hz when reading 1 channel and 2 Hz when reading all 4 channels 5 2 6 Set temp C Use this button to set the temperature of the ambient water in C This field is used only in fiber optic measurements and is disabled enabled together with the fiber optic button status OFF ON The default value is 20 C If the temperature cannot be kept during measurements within the entered value with 0 2 C choose to compensate the fiber optic oxygen signal with the measured temperature signal by enable the checkmark Analog Comp see 5 2 14 1 page 37 If Analog Comp is enabled the Set temp C field gets disabled and will be updated with the measured temperature The fiber optic oxygen value will now be real time compensated for the temperature Page 28 of 55 L oLico LOLIGOSYSTEMS COM 5 2 7 Next gt button Screen 28 Use this button to accept the calibration settings and move on to setting up a resp experiment see 5 2 14 page 37 Before proceeding to the setup the user is prompted to enter a data file name and path see Screen 28 The path specifies the location of the main data file and a folder containing raw data files from the experiment The path is by default the installation directory of AutoResp4 Auto
13. predetermined to approx 1 2 Hz by the OXY 4 mini instrument Please note The look and functionality of the Ambient Oxygen field and the Oxygen graph field are similar to those of Screen 27 Screen 29 An AutoResp 4 File Help Sample Frequency Barometic pressure hPa DO sat kPa Fiber Optic ON Set temp C D LEE 8 a SS d GE 1 Hz 1013 20 859 lt gt 20 00 Chamber oxygen 1 Enable Chamber oxygen 2 Enable 7 Oxygen graph kPa 10 51 Mov Avg pO2 kPa Mov Avg pO kPa Gaid 3 SE 14 16 I point lt 10 58 1 point lt 14 22 25 14 94 LO Temp C Phase LO Temp C Phase 15 68 d acinentnmeni y r vdana praa 7 d Lock LO J 120 00 0 Lock LO F 120 00 0 HI Temp C Amplitude HI Temp C Amplitude Lock HI J 20 00 0 Lock HI 20 00 de Chamber oxygen 3 _ Enable ES Chamber oxygen 3 Enable a Mov Avg pO kPa Mov Avg poz kPa 1 point l 15 00 1 point v 15 73 LO Temp C Phase LO Temp C Phase Lock LO e 20 00 0 Lock LO 20 00 f HI Temp C Amplitude HI Temp C Amplitude Lock HI St 0 Lock HI J 20 00 o Ambient oxygen Ir Temperature Analog Comp 7 Mov Avg Input O DO kPa Mov Avg Input O 1 point si 2 78 11 64 1 point si 2 96 LO input Ni LO pO kPa LO input LO Temp C Lock LO sl 0 00 S 10 00 Lock LO 10 00 F hf HI input WA Hip kPa Hi input WA Hi Temp C pn Lock HI F 5 00 g 120 96 Lock HI S 15 00 110
14. 0 00 10 39 23 10 40 23 Page 35 of 55 L oLico LOLIGOSYSTEMS COM 5 2 13 Chamber oxygen field 1 4 5 2 13 1 Mov Avg button Use this button to select if the chamber oxygen input signal should be averaged or not The default value is 1 point which means no averaging of the input If any other value is chosen the chamber oxygen input will be averaged and the averaged values will be used for all subsequent computations screen and file outputs Use moving average if you wish to smoothen the chamber oxygen Signal Please note No calibrated values will be displayed either on screen or in the data file before sufficient samples have been collected to compute the moving average E g if the moving average is set to 10 points and all four channels are enabled there will be no chamber oxygen values the first ten samples equivalent to the first twenty seconds of samples 5 2 13 2 Enable channel By clicking on this field the channel gets enabled and oxygen data will be received Please note It is only possible to enable a channel when a sensor is connected to the OXY 4 mini instrument Please note If a sensor is not used then disable the channel This will increase the lifetime of the sensor 5 2 13 3 pO kPa indicator This field displays the chamber oxygen pressure from the OXY 4 fiber optic instrument from PreSens relative the calibration 5 2 13 4 Lock LO button Press this button to calibrate the OXY 4 min
15. 02 30 20 D Som 10 0 LI et PEN bem Kod Gen et Lae Mia i TXR TERR CI poh aa D E sree at U SES Ke pclae Temp C ASSOSS KS EIS ERR i WA 11 04 23 11 14 23 E r 58 86 5 4 2 Oxygen graph field This field shows the measured oxygen values vs time By right clicking the graph the user can choose which chamber graph is to be shown It is also possible to show the ambient oxygen as a graph and modify the time interval on the x scale 10 30 and 90 min Another option is to save the graph to a file and to show the experiment settings Page 42 of 55 L oLico LOLIGOSYSTEMS COM 5 4 3 Graph field This field shows some different graphs for the experiment As default the current temperature vs time graph is shown The user can choose between the following graphs by clicking with the mouse on the view button in the menu MO vs time MO vs avg Chamber O Avg temperature vs time Current temperature vs time R vs time Current R vs time The MO gt avg temperature and the R vs time graph shows data from the whole experiment The current temperature and the current R vs time graph shows data for the last 10 30 or 90 min depended on the x scale from the oxygen graph see 5 4 2 By right clicking on the graph the user can save the shown graph to a file 5 4 4 Status field 5 4 4 1 Loop remaining s This field shows the remaining seconds left of the current measurement loop The loop is def
16. 19 221 18 99 98 12 20 423 20 403 20 382 20 357 20 329 20 298 20 262 20 222 20 178 20 128 20 072 19 904 19 686 19 406 19 274 19 05 98 39 20 478 20 459 20 437 20 412 20 384 20 353 20 317 20 277 20 233 20 183 20 127 19 958 19 739 19 459 19 326 19 10 9865 20 534 20 514 20 492 20 467 20 439 20 408 20 372 20 332 20 288 20 237 20 182 20 012 19 793 19 512 19 378 19 15 98 92 20 589 20 570 20 548 20 523 20 495 20 463 20 427 20 387 20 342 20 292 20 236 20 066 19 846 19 564 19 431 19 20 99 19 20 645 20 625 20 603 20 578 20 550 20 518 20 482 20 442 20 397 20 347 20 291 20 121 19 900 19 617 19 483 19 25 99 45 20 700 20 681 20 658 20 633 20 605 20 573 20 537 20 497 20 452 20 402 20 345 20 175 19 953 19 670 19 536 19 30 p99 72 20 756 20 736 20 714 20 689 20 660 20 628 20 593 20 552 20 507 20 456 20 400 20 229 20 007 19 722 19 588 19 36 99 99 20 811 20 791 20 769 20 744 20 716 20 684 20 648 20 607 20 562 20 511 20 454 20 283 20 060 19 775 19 640 19 41 100 25 20 866 20 847 20 825 20 799 20 771 20 739 20 703 20 662 20 617 20 566 20 509 20 337 20 114 19 828 19 693 19 46 100 52 20 922 20 902 20 880 20 855 20 826 20 794 20 758 20 717 20 671 20 620 20 563 20 391 20 167 19 881 19 745 19 51 100 79 20 977 20 958 20 935 20 910 20 881 20 849 20 813 20 772 20 726 20 675 20 618 20 445 20 221 19 933 19 797
17. 2 8 8 HI input V LOLIGOSYSTEMS COM Use this field to enter the high input voltage from the Input V indicator during the chamber oxygen calibration Please note The field is limited to values between OV to 5V 5 2 8 9 HI pO kPa Use this field to enter the oxygen pressure during the high level oxygen calibration of the chamber oxygen input Please note The field is limited to values between 0 00 kPa to 60 00 kPa 5 2 9 Ambient oxygen field 5 2 9 1 Mov Avg button Use this button to select if the ambient oxygen input signal should be averaged or not The default value is 1 point which means no averaging of the input If any other value is chosen the ambient oxygen input will be averaged and the averaged values will be used for all subsequent computations screen and file outputs Use moving average if you wish to smoothen the ambient oxygen Signal Please note No calibrated values will be displayed either on screen or in the data file before sufficient samples have been collected to compute the moving average E g if the moving average is set to 30 points and the sample rate is set to 10 Hz there will be no ambient oxygen values the first 30 samples equivalent to the first three seconds of samples 5 2 9 2 Input V indicator This field displays the actual voltage input 5 2 9 3 pO kPa indicator This field displays the actual calculated oxygen partial pressure from the voltage input 5 2 9 4 Lock LO button T
18. 60 kPa 5 3 5 3 Hysteresis kPa Use this field to change the oxygen hysteresis value Use a hysteresis value gt 0 kPa to ensure that the O2 N gt valve does not flicker on the sharp edge of the setpoint If the hysteresis pressure is 0 kPa the O2 N gt valve is very susceptible to electronic noise on the ambient oxygen channel Please note The field is limited to values between O kPa to 30 kPa 5 3 5 4 Hypoxic Hyperoxic Use these buttons to select if the system must maintain the setpoint as a maximum Hypoxic or a minimum Hyperoxic oxygen pressure Page 40 of 55 L oLico LOLIGOSYSTEMS COM 5 3 6 Temperature control field 5 3 6 1 Checkbox Use the checkbox to enable or disable the TEMP relay on the DAQ 4 instrument If the checkbox is checked AutoResp 4 can control the temperature in the ambient water as specified in the temperature control field 5 3 6 2 Setpoint C Use this field to set a temperature level in the ambient water that the system Should maintain during an experiment Please note The field is limited to values between 2 C to 100 C 5 3 6 3 Hysteresis C Use this field to change the temperature hysteresis value Use a hysteresis value gt 0 C to ensure that the cooler heater pump does not flicker on the sharp edge of the setpoint If the hysteresis temperature O C the cooler heater pump is very susceptible to electronic noise on the temperature channel Please note The f
19. 669F 2 o Ri NI DAQmx Devices NI ee RASH Sera SOP e 2 W NIUSB 6215 Devi DAQmx Device PXI PXI System Unidentified Lu Basics Gg Scales a Software Q Remote Systems What do you want to do ab Run the NI DAQmx TestPanels Remove the device View or change device properties SI Calibration il Im QXAGT IEW SL 9 56AM Make sure the NI USB 6215 is installed as device name Devi If so the DAQ 4 instrument is now properly correct installed and ready for use with AutoResp 4 29 Remove CD labelled AutoResp 4 30 You have now installed AutoResp 4 and all necessary drivers AutoResp 4 is now ready for use see Using AutoResp 4 page 25 for help starting AutoResp 4 Page 17 of 55 L oLico LOLIGOSYSTEMS COM 3 4 OXY 4 mini instrument 3 4 1 Front side CH1 Fiber optic oxygen input for chamber 1 CH2 Fiber optic oxygen input for chamber 2 CH3 Fiber optic oxygen input for chamber 3 CH4 Fiber optic oxygen input for chamber 4 Connect an oxygen sensor e g oxygen needle type probe to an input LINE Indicates instruments ON OFF 3 4 2 Back side Power connector Power input for the OXY 4 mini instruments Connect the instrument to a grounded 230 VDC power supply using a standard pc type cable with a grounded wall plug Power button Turns instrument on and off DATA RS 232 interface male Connect the OXY 4 mini instrument to the PC via a RS 232
20. CK THE APPROPRIATE BUTTON TO CANCEL THE INSTALLATION PROCESS DO NOT INSTALL OR USE THE SOFTWARE AND RETURN THE SOFTWARE WITHIN THIRTY 30 DAYS OF RECEIPT OF THE SOFTWARE INCLUDING ALL ACCOMPANYING WRITTEN MATERIALS ALONG WITH THEIR CONTAINERS TO THE PLACE YOU OBTAINED THEM ALL RETURNS SHALL BE SUBJECT TO NI S THEN CURRENT RETURN POLICY 1 Definitions 4s used in this Agreement the following terms have the following meanings cl accept the License Aoreementtz I do not accept the License Agreement s 10 Then click Next On Screen 8 a summary is given of the products to be installed Screen 8 ite AutoResp 4 Start Installation Review the following summam before continuing Adding or Changing e AutoRespl 4 Files e N i 5 5 e NI LabvlEe Run Time Engine 7 1 1 e NI Measurement amp Automation Explorer 4 2 Click the Next button to begin installation Click the Back button to change the installation settings 11 Click Next Page 9 of 55 On Screen 9 the status of the installation is shown Screen 9 7 AutoResp 4 Overall Progress Curmently installing NI Assistant Framework LabVIEW Code Generator 6 1 Part 1 of 58 vc Back Hext gt gt The installation may take a while When installation is complete Screen 10 becomes present Screen 10 Installation Complete The installer has finished updating your system lt i Back Netz Cancel 12 Click Next to end installation of
21. OLIGOSYSTEMS COM 5 3 4 Chamber field 1 4 5 3 4 1 Resp vol I Use this field to specify the volume of water used for the calculation of the MO gt value Resp vol chamber volume tube volume volume of organism s To specify the Resp vol fill the chamber and the tubes with water and measure the weight on a scale Now empty the chamber and the tubes for water and measure the weight again The difference between the two measurements will be the weight of the chamber volume and the tube volume Multiply the calculated weight with the mass specific volume of water for finding the volume for the chamber and the tubes To find the volume of the organism measure the weight of the organism and multiply with the mass specific volume of the organism Please note This field is limited to values between 0 to 2500 I 5 3 4 2 Wet weight g Use this field to specify the weight of the experimental organism s Please note This field is limited to values between 0 001g to 100 kg 5 3 5 Oxygen control field 5 3 5 1 Checkbox Use the checkbox to enable or disable the O2 N gt relay on the DAQ 4 instrument If the checkbox is checked AutoResp 4 can control the O pressure in the ambient water as specified in the oxygen control field 5 3 5 2 Setpoint kPa Use this field to set an oxygen level in the ambient water that the system should maintain during an experiment Please note The field is limited to values between O kPa to
22. Resp4Data The current time and date xls is suggested as a filename in the prompt Both the path and filename can be altered by choice The system suggests two file extensions xls and txt The xls extension will create a tabulator separated text file which is readable by Microsoft Excel The txt extension will create a semicolon separated text file which is readable by any Microsoft Windows PC In addition to the data file the system will also generate a folder named as the data file The dot in the filename will however be replaced by an underscore _ in the folder name In this folder files containing raw data will be generated for each measurement loop The raw data files contain data from the measure period on which the calculated values in the main data file are based Save data file as Gemi AutoResp4Data Seneste dokumenter Skrivebord Dokumenter dp Denne computer Filnavn 06 06 2007 11 36 52 xl D Netveerkssteder Filtype Excel compatible or delimited text file Tale v Page 29 of 55 L oLico LOLIGOSYSTEMS COM 5 2 8 Chamber oxygen field 1 4 5 2 8 1 Mov Avg button Use this button to select if the chamber oxygen input signal should be averaged or not The default value is 1 point which means no averaging of the input If any other value is chosen the chamber oxygen input will be averaged and the averaged values will be used for all subsequent computations scree
23. Self Test A Test Panels fJ ResetDevice ini Create Task amp Configure TEDS E3 Hide Help E Q My System a A Data Neighborhood Name Value Geck fezi G Devices and Interfaces Biserial Number 0xE669F2 NI DAQmx Devices NI W NIUSB 6215 Dev Isimu DAQmx Device si NI USB 6215 Devi b Basics BI PXI System Unidentified e BM Scales E Software H N a EN Remote Systems What do you want to do ab Run the NI DAQmx Test Panels af Remove the device View or change device properties D te E ES pc Nesen 0 215 De QZMA IALA SE zm s RE Please note By changing the NI USB 6215 device to devi another applications using devices from National Instruments might not work properly anymore 6 1 2 Another USB device was improperly removed If an USB device like a USB memory stick is not properly removed while AutoResp 4 is running there can be a connection problem with the DAQ 4 instrument To solve this problem close AutoResp 4 Then disconnect the USB cable between the PC and the DAQ 4 instrument Wait 30 seconds Then connect the cable and wait When the green diode on the front side of the DAQ 4 instrument is flashing the connection is re established Choose Continue experiment to avoid loosing data 6 2 Relays are not working Make sure the DAQ 4 instrument is connected with a power cable and the Power button is ON Page 48 of 55 7 iIndex Oa
24. aiming at combining the best of both 1 closed and 2 flow through respirometry Reference Steffensen J F 1989 Some errors in respirometry of aquatic breathers how to avoid and correct for them J Fish Physiol Biochem 6 49 59 The experimental animal is placed in a closed chamber respirometer immersed in an ambient tank A recirculation pump ensures proper mixing of the water inside the respirometer and adequate flow past the oxygen probe A second pump can change the water inside the respirometer with ambient water During measurements of oxygen consumption this flush pump is turned off and the systems operates like 1 closed respirometry Then the pc controlled flush pump turns on pumping ambient water into the respirometer and bringing the oxygen content back to pre measurement values In this way problems with accumulating metabolites and severe changes in oxygen level due to animal respiration are avoided As with open respirometry the duration of the experiment is in principle unlimited However the most important advantage is the great time resolution of this method Oxygen consumption rates of animals can be determined for every 10 minutes over periods of hours or days making the system extremely suited for uncovering short term variations minutes in metabolism In summary our systems for respirometry are developed for prolonged and automatic measurements of oxygen consumption rate in a controlled laboratory enviro
25. d to values between 0 C to 100 C 5 2 10 7 Lock HI button This button can be used to copy the actual value from the Input V indicator to the HI input V field instead of manually reading and retyping the value 5 2 10 8 HI input V Use this field to enter the high input voltage from the Input V indicator during the high temperature calibration Please note The field is limited to values between OV to 5V 5 2 10 9 HI Temp C Use this field to enter the temperature during the high temperature calibration Please note The field is limited to values between 0 C to 100 C Page 33 of 55 L oLico LOLIGOSYSTEMS COM 5 2 11 Chamber Oxygen graph field This graph shows the oxygen pressure vs time measured for the 4 chambers going back 1 min By right clicking the graph it is possible to show hide the output data from a chamber It is also possible to show hide the ambient oxygen This is only possible if the Play button is ON When OFF and right clicking it is possible to copy data and export a simplified image of the graph Right to the graph there are six fields where current data are shown The first field shows the chosen units which can be altered by the unit button It is possible to change the color for every signal by clicking on the color field for every signal 5 2 11 1 Save button Use this button to save the data displayed in the Oxygen graph Furthermore the temperature will be saved When pres
26. dware Wizard Windows will search for curent and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your pennission Can Windows connect to Windows Update to search for software Yes this time only O Yes now and every time connect a device Gei No not this time Click Next to continue 22 Click on No not this time and then on Next Page 13 of 55 li OLIGO Screen 16 LOLIGOSYSTEMS COM Found New Hardware Wizard This wizard helps you install software for LISB 62 1x Loader H your hardware came with an installation CD lt a or floppy disk insert it now What do you want the wizard to do Gei Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue E 23 Click on Install the software automatically Recommended and then on Next Screen17 _ Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for bY USB 62 lx Loader amp Click Finish to close the wizard lt Back Finish cancel 24 Click Finish Page 14 of 55 L oLico Screen 18 LOLIGOSYSTEMS COM 4 Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or
27. es 22 4 3 Intermittent flow respirometry or open closed respirometr 23 5 USING AUCOR CSD 2 EE 25 Dd AUD EE 25 5 2 Calibration and selection Of OXYGEN INPUT ss sssssssssrssrsnrrerrerrnnrsrrnnnnnrnrrnrrnrennn 27 5 3 iol HU em o gt 40 lt 1 9 4 io gedeien 38 5 4 RUNNING ug Rn ER 42 6 TROUPE SINC el e rE A E TA E EE AA ae tars 46 6 1 No connection to the DAQ 4 metrument 46 6 2 Relays are NOE WOKING EE 48 te EE 49 PDD Gre EE 50 8 1 S ces TEE 50 8 2 EECHER e iulm ER eer 51 8 3 DAQ 4 TSE CLOW Bn VUE 52 E Bh LEE el eee ee ee ee ee ee eee ee ee er ee er ee 55 8 5 OREG E 55 8 6 O74 Tisch leien Dn TE E 55 Page 2 of 55 L oLico LOLIGOSYSTEMS COM 3 Installation procedure for DAQ PAC F4X 3 1 General e AutoResp 4 is a piece of PC software designed to perform automated intermittent flow respirometry together with the DAQ 4 instrument e AutoResp 4 requires Windows XP running on a PC with an Intel Pentium IV processor of minimum 2 66 GHz or equivalent The PC must have at least 256 MB memory and at least 1GB of free hard disk space A minimum resolution of 1024x768 pixels is required e AutoResp 4 communicates with the DAQ 4 instrument via the accompanying USB cable which must be connected e AutoResp 4 can handle two different types of oxygen inputs Either 0 5 VDC analog input OXY CH1 4 or serial inputs when using OXY 4 mini instrument manufactured by PreSens e If chamber oxygen is measured usin
28. g data cable PC USB port for PC communication Use the USB cable to connect the DAQ 4 instrument to the PC LEDS Relay status LEDs IMPORTANT DO NOT connect DAQ 4 instrument to PC before installation of driver from Measurement amp Automation software from National Instruments is completed The software will be installed together with the installation of AutoResp 4 see AutoResp 4 software for Windows page 6 Page 4 of 55 L oLico LOLIGOSYSTEMS COM 3 2 2 Back side FLUSH Relay socket for 110 230VAC max 3A flush pumps Connect one or more pumps by using one of the 4 fold power strips RECIRC Relay socket for 110 230VAC max 3A recirculation pump Connect one or more pumps by using one of the 4 fold power strips O2 N2 Relay socket for 110 230VAC max 3A for solenoid valve Use oxygen or nitrogen for hyperoxic or hypoxic control Connect the solenoid valve by using the push in fittings TEMP Relay socket for 110 230VAC max 3A heating cooling pump Connect the heating cooling pump by using the adapter cable 100 240VAC 50 60 Hz Power input for the DAQ 4 instrument Connect the instrument to a grounded 110 230VAC power supply using a standard pc type cable with a grounded wall plug Power button Turns the instrument on and oft IMPORTANT DO NOT connect any of the relay sockets to gt 3 amps equipment USE grounded outlets only For further information about the DAQ 4 instrument see 8 3 DAQ 4 Instruct
29. g the OXY 4 mini instrument one RS232 male DB9 connector must be available on the PC If not available use an USB 232 adapter e g USB Serial Adapter manufactured by VSCOM e If using the OXY 4 mini instrument turn this on before starting AutoResp 4 Input labels see Table 1 Table 1 DAQ 4 instrument label AutoResp 4 signal name OXY CH1 Chamber oxygen 1 OXY CH2 Chamber oxygen 2 OXY CH3 Chamber oxygen 3 OXY CH4 Chamber oxygen 4 OXY AM Ambient oxygen TEMP Temperature Output labels see Table 2 Table 2 DAQ 4 instrument label AutoResp 4 indicator name FLUSH Flush RECIRC Recirc D2 N2 Ambient O kPa TEMP Temperature C Page 3 of 55 L oLico LOLIGOSYSTEMS COM 3 2 DAQ 4 instrument 3 2 1 Front side OXY CH1 Analog oxygen input for chamber 1 The input range is 0 5 VDC OXY CH2 Analog oxygen input for chamber 2 The input range is 0 5 VDC OXY CH3 Analog oxygen input for chamber 3 The input range is 0 5 VDC OXY CH4 Analog oxygen input for chamber 4 The input range is 0 5 VDC Connect analog oxygen inputs e g from an OXY REG via pin 1 and pin 4 GND in the input connector OXY AM Analog oxygen input for ambient water The input range is 0 5 VDC Connect analog oxygen input to the OXY REG instrument via the accompanying data cable TEMP Analog temperature input for ambient water The input range is 0 5 VDC Connect analog temperature input to the TMP REG instrument via the accompanyin
30. gt lte oxygen concentration at time tp mg O gt liter O 2 t oxygen concentration at time t mg O gt liter V respirometer volume minus volume of experimental animal liter t t t hour BW body weight of experimental animal kg An advantage of this method is its simplicity A disadvantage is that the measurements are never made at a constant oxygen level due to the continuous use of oxygen by the animal inside the respirometer This might cause problems when interpreting data since animal respiration often changes with ambient oxygen partial pressure Furthermore metabolites from the experimental animal Le CO accumulate in the water thus limiting the duration of measurements This limited time for measurements prevents the experimental animal to recover from initial handling stress that often increase fish respiration Significantly and for several hours thus overestimating oxygen consumption rates Page 21 of 55 L oLico LOLIGOSYSTEMS COM 4 2 Flow through respirometry or open respirometry This is a more sophisticated method for oxygen consumption measurements Experimental animals are placed in a flow through chamber with known flow rate Oxygen is measured in the inflow and outflow and oxygen consumption rate can be calculated as VO F O2 in TO leur BW VO oxygen consumption rate mg O2 kg hour F water flow rate l hour Oo in oxygen content in water inflow mg O gt liter
31. her older devices Do not install HI DAG 7 0 or later if your existing applications include unsupported components Refer to the NI DAQ Readme file on the distribution 6 Click Next 7 Select destination directory for AutoResp 4 and for the National Instruments driver Screen 6 Y AutoResp 4 LG m a Destination Directory Select the primary installation directory All software will be installed in the following locationis To install software into a different locationis click the Browse button and select another directory Directory for 4utoRespl 4 C Program Files Auto Resp 4 Directory for National Instruments products C Program Files National Instruments 8 Click Next Page 8 of 55 LOLIGOSYSTEMS COM 9 If you accept the License Agreement please select I accept the License Agreement s Screen 7 SJ AutoResp 4 License Agreement You must accept the license s displayed below to proceed NATIONAL INSTRUMENTS SOFTWARE LICENSE AGREEMENT INSTALLATION NOTICE THIS IS A CONTRACT BEFORE YOU DOWNLOAD THE SOFTWARE AND OR COMPLETE THE INSTALLATION PROCESS CAREFULLY READ THIS AGREEMENT BY DOWNLOADING THE SOFTWARE ANDOR CLICKING THE APPLICABLE BUTTON TO COMPLETE THE INSTALLATION PROCESS YOU CONSENT TO THE TERMS OF THIS AGREEMENT AND YOU AGREE TO BE BOUND BY THIS AGREEMENT IF YOU DO NOT WISH TO BECOME APARTY TO THIS AGREEMENT AND BE BOUND BY ALL OF ITS TERMS AND CONDITIONS CLI
32. his button can be used to copy the actual value from the Input V indicator to the LO input V field instead of manually reading and retyping the value 5 2 9 5 LO input V Use this field to enter the low input voltage from the Input V indicator during the ambient oxygen calibration Please note The field is limited to values between OV to 5V Page 31 of 55 L oLico 5 2 9 6 LO pO kPa LOLIGOSYSTEMS COM Use this field to enter the oxygen pressure during the low level oxygen calibration of the ambient oxygen input Please note The field is limited to values between 0 00 kPa to 60 00 kPa 5 2 9 7 Lock HI button This button can be used to copy the actual value from the Input V indicator to the HI input V field instead of manually reading and retyping the value 5 2 9 8 HI input V Use this field to enter the high input voltage from the Input V indicator during the ambient oxygen calibration The lower Lock button can be used to copy the actual value from the Input V indicator to the HI input V field instead of manually reading and retyping the value Please note The field is limited to values between OV to 5V 5 2 9 9 HI pO kPa Use this field to enter the oxygen pressure during the high level oxygen calibration of the ambient oxygen input Please note The field is limited to values between 0 00 kPa to 60 00 kPa 5 2 10 Temperature field 5 2 10 1 Mov Avg button Use this button to select if the te
33. i instrument in a 0 air saturation O sample Please note It is important not to push the Lock LO button before the Phase and Amplitude indicators has stabilized Please note A full calibration must consist of a 0 calibration and a 100 calibration before the oxygen readouts are reliable Please note The calibration will be written down on an EEPROM in the OXY 4 mini instrument If you want to use the OXY 4 mini instrument with the software from PreSens a new calibration may be done using this software 5 2 13 5 LO Temp C Use this field to specify the temperature during calibration of the OXY 4 fiber optic instrument with the 0 air saturation O solution Page 36 of 55 L oLico LOLIGOSYSTEMS COM 5 2 13 6 Lock HI button Press this button to calibrate the OXY 4 mini instrument in a 100 air saturation O sample Please note It is important not to push the Lock HI button before the Phase and Amplitude indicators has stabilized Please note A full calibration must consist of a 0 calibration and a 100 calibration before the oxygen readouts are reliable Please note The calibration will be written down on an EEPROM in the OXY 4 mini instrument If you want to use the OXY 4 mini instrument with the software from PreSens a new calibration may be done using this software 5 2 13 7 HI Temp C Use this field to specify the temperature during calibration of the OXY 4 fiber optic instrument with the 100 a
34. ield is limited to values between 0 C to 49 99 C 5 3 6 4 Cool Heat Use these buttons to select if the system must maintain the setpoint as a maximum Cool or a minimum Heat temperature 5 3 7 Start experiment button Use this button to accept the setup settings as they are displayed on Screen 30 and proceed to Screen 31 5 3 8 Menus in Screen 27 Screen 29 and Screen 30 5 3 8 1 File gt Exit Exits AutoResp 4 without saving changes to the calibration screens 5 3 8 2 File gt Continue experiment Continues the last experiment if stopped by user Data on the graphs will only be in memory when AutoResp 4 has not been shut down Please note This option is only available if a calibration has been made and the user has stopped a running experiment 5 3 8 3 Help gt About Displays contact information about Loligo Systems Page 41 of 55 L oLico LOLIGOSYSTEMS COM 5 4 Running experiment 5 4 1 General about Screen 30 On Screen 31 the intermittent respirometry experiment is running Screen 31 An AutoResp 4 File Edit View Help ee Status Remaining time Lei ua J FLUSH State e ek Oxygen kPa Chamber 1 Chamber 2 1 Br eed AD a ay RX Ka ei WR ei t 4 E eee Gu Ee U PE DL el aoe eel A Som Era Cras P Sey Berea I 11 04 23 11 14 23 J 100 ge Chamber 3 KPa D IER ms Chamber 4 60 O2 kPa sa EI Dee J 40 80 Current Temp C Ambient
35. ined as the summed duration of flush wait and measure periods 5 4 4 2 Loop number s This field shows the actual state of the loop and the number of loops The letter F means flush W means wait and M means measure Behind the letter a number is shown which shows the actual number of the loop 5 4 4 3 Flush indicator This diode indicates if the flush pump is activated or not The flush pump is running only in a flush period 5 4 4 4 Recirc indicator This diode indicates if the recirculation pump is activated or not The recirculation pump is running in a flush and a measure period Page 43 of 55 L oLico LOLIGOSYSTEMS COM 5 4 5 Ambient measure field 5 4 5 1 Ambient O2 kPa This field shows the actual measured ambient water oxygen pressure 5 4 5 2 Temperature C This field shows the actual measured temperature 5 4 5 3 Oxygen regulation This field shows if the oxygen regulator is working to maintain the oxygen level in the ambient tank as hypoxic or hyperoxic see 5 3 5 Oxygen control field page 40 5 4 5 4 Temperature regulation This field shows if the temperature regulator is working to maintain the temperature level in the ambient water see 5 3 6 Temperature control field page 41 5 4 6 Chamber measure field 1 4 5 4 6 1 Chamber O kPa This field shows the actual measured oxygen pressure for the chamber 5 4 6 2 MQ field This field shows the calculated MO value for the cha
36. ion manual page 52 Page 5 of 55 L oLico LOLIGOSYSTEMS COM 3 3 AutoResp 4 software for Windows The following steps will explain how to install AutoResp 4 and drivers on your computer 1 Insert the CD labelled AutoResp 4 and wait until you see Screen 1 If you do NOT se the screen browse to the root of your CD and double click on the icon labelled AR4 exe Screen 1 AutoResp 4 Installation The installation is initializing Please walt weem 2 After the installation is initialized click Setup to start the installation Screen 2 Win ip Self Extractor AutoResp exe Ge Click Setup to install AutoResp 4 Cancel About Page 6 of 55 LOLIGOSYSTEMS COM 3 Click Install to start the installation of AutoResp 4 and all necessary drivers Screen 3 AutoResp 4 Installation Readme Please read the following Information welcome to the AutoResp 4 Installer You will now install Auto esp 4 and all necessary drivers If asked if pou want to restart please don t click on restart until later our pe should not be restarted until the entire installation e completed 4 Click Next to start installation of AutoResp 4 Screen 4 Welcome to the AutoRespl 4 installer Please follow the instructions on screen and wait until you are asked to restart Your computer Back 5 Click Next Page 7 of 55 l oLico LOLIGOSYSTEMS COM
37. ir saturation O solution 5 2 13 8 Phase indicator This field indicates the phase angle of the optical signal that the OXY 4 mini instrument uses for computations of the chamber oxygen pressure For further information about the OXY 4 mini instrument see 8 6 OXY 4 Instruction manual page 55 5 2 13 9 Amplitude indicator This field indicates the amplitude of the optical signal that the OXY 4 mini instrument measures The signal strength is an indicator for the sensor condition See the manual of the OXY 4 fiber optic instrument from PreSens for further explanation 5 2 14 Temperature field When the fiber optic button is ON the Temperature field gets changed from Screen 27 page 27 to Screen 29 page 35 A checkmark appears named Analog Comp 5 2 14 1 Analog Comp Use this checkmark to enable disable the real time compensation of the fiber optic oxygen signal with the analog measured temperature If the temperature changes with 0 2 C the new temperature will be send to the fiber optic instrument Page 37 of 55 5 3 Setup experiment 5 3 1 Screen 30 P AutoResp 4 Help File name Setup Flush period s Si e 0 4855 0 solubility mo ka General about Screen 30 On Screen 30 the intermittent respirometry experiment is configured Wait period s Een These are the notes about the experiment f 300 4 Measure period ei Chamber 1 Resp vol 1 1 0000 Wet weigh
38. l oLico LOLIGOSYSTEMS COM USER MANUAL DAQ PAC F4X With OXY 4 mini 1 List of parts e DAQ 4 instrument o AutoResp 4 o Power Cord o USB cable o 4 fold power strips for pumps qty 2 o User manual e OXY 4 mini instrument o Power Cord o RS232 cable o User manual o PC operating software for Windows 98 00 ME NT XP e OXY REG instrument o Galvanic O probe Maintenance kit for O probe Power Cord Data cable Solenoid valve w push in fittings PU tubing Air stone User manual O O O O E 3 2 e TMP REG instrument o Pt100 probe Power Cord Data cable Submersible Eheim pump Soft PVC tubing Stainless steel cooling coil Adapter cable for pump User manual O O O O O sO 33 Page 1 of 55 L oLico LOLIGOSYSTEMS COM 2 Contents 1 ETS CAO Fs Eeer ee EA T P eege ee eases esses ee 1 2 Co iss eh ee eT ee ee ee ee 2 3 Installation procedure for DAQ PAC F4X sssssssssssrssrerrsrrnsnrrnrrreerrereererrrerrerrerrererenre 3 3 1 Ene TN E 3 3 2 R NNEN 4 3 3 AutoResp 4 software for Windows 6 3 4 SPOP SCPC EE 18 3 5 TMERRE GHA Ra in SEET 19 3 6 OX REG INS ONE et See EE EE e 20 4 Introduction to intermittent flow respirometry cccceeeee cece cece eeeeee tense eee eeeeeeeeeeeees 21 4 1 Closed respirometry or constant volume respirometry c cece cece cece eeeeeeeeeeees 21 4 2 Flow through respirometry or OPEN respirometrv cece cece eeeeeeeeeeeeeeeenne
39. les containing raw data will be generated for each measurement loop The raw data files contain data from the measure period on which the calculated values in the main data file are based Please note The start experiment button will be disabled until a valid path filename is selected Page 38 of 55 L oLico 5 3 3 Setup field LOLIGOSYSTEMS COM 5 3 3 1 Flush period s Use this field to specify the duration of flush periods in seconds during the intermittent flow respirometry experiment Please note The field is limited to values between 0 s to 35 weeks 5 3 3 2 Wait period s Use this field to specify the duration of the wait periods in seconds during the intermittent flow respirometry experiment Please note The field is limited to values between 1 s to 35 weeks 5 3 3 3 Measure period s Use this field to specify the duration of the measure periods during the intermittent flow respirometry experiment Please note The field is limited to values between 1 s to 35 weeks 5 3 3 4 O solubility mg O2 I kPa Use this field to specify the oxygen solubility of sample water Please note This field is limited to values between 0 to 0 75 mg L kPa The default value is 0 4855 corresponding to a 15 C fresh water For further information see the appendix page 50 and 51 5 3 3 5 Note field In this field the user can write a note about the experiment The note is saved to the data file Page 39 of 55 L oLico L
40. ligo Systems make sure the input range is 0 5 VDC The signal must be on pin 1 and GND on pin 4 on an input connector Using the DAQ 4 in other applications It is possible to use the DAQ 4 instrument with other applications than AutoResp 4 See Table 3 for input connections and the wiring diagram on Figur 1 Table 3 DAQ 4 connector NI USB 6215 pins OXY CH 1 pay AS OXY CH 2 OXY CH 3 a ee E Page 52 of 55 LOLIGOSYSTEMS COM Figur 1 AW3L eNn eo JHII HSMAI TLAGLAD TDHLNOI i i i i i i i i i i i i X9 PE X8 PE X7 PE dwal 2N 20 ORLO20 HG V s zA w A o h S Seite cl zk ee amp 1qe3 Yoddty pM joerg D se es oe Bt MERE Ae si KT g S lt H 8 Joerg Ze S 5 umoug Le a EN i d E L 8 EE ba et UMOJG BE D m D x 1 I x E goe D z WER D 1 i bw a 1 p d JTA x ps pue ge DH p l z a s l yq sT i WER Je S i os s kal gt j 4 Le j D D al ms H MOTT A uUsSuE i Gei s FEI uMoug E i Ds T 1 rH SNISVO LNAWNYLSNI E ee ee ee ae ee eee ee eee gi CHL WY AXO HO AXO HI AXO eHO AXO FHO AXO LAGNI HOSNIS Jd ZHE9 8S JS ASEc OOT A Tedd AS Page 53 of 55 For using the LEDs and the rela
41. mber as described in background see 4 Introduction to intermittent flow respirometry page 21 The MO gt value is calculated as follows MO ea 3600 O solubility mus stopa resp eee h k l kPa S wet weight Where slope is calculated as follows Ka O 7 Oza slope e e A me Me Please note MO is only calculated in each measurement period e g 29 MO values after 30 seconds measure period at 1 Hz Page 44 of 55 L oLico 5 4 6 3 R field LOLIGOSYSTEMS COM This field shows the calculated R linear correlation coefficient value for the chamber when MO is calculated The R value can be used to validate the linear fit of the O curve 5 4 7 Menus in Screen 31 5 4 7 1 File gt Stop experiment This option stops the current experiment Please note To immediately turn on the flush pump use this option 5 4 7 2 Edit gt Oxygen While the experiment is running the user can use this option to alter the oxygen regulation This can be done by changing the setpoint the hysteresis or the mode hypoxic hyperoxic 5 4 7 3 Edit gt Temperature While the experiment is running the user can use this option to alter the temperature regulation This can be done by changing the setpoint the hysteresis or the mode cool heat 5 4 7 4 View By this option the user decides which graph is to be shown in the graph field see 5 4 3 Graph field on page 43 5 4 7 5 Help gt About Displays contact info
42. mperature input signal should be averaged or not The default value is 1 point which means no averaging of the input If any other value is chosen the temperature input will be averaged and the averaged values will be used for all subsequent computations screen and file outputs Use moving average if you wish to smoothen the temperature signal Please note No calibrated values will be displayed either on screen or in the data file before sufficient samples have been collected to compute the moving average E g if the moving average is set to 30 points and the sample rate is set to 10 Hz there will be temperature values the first 30 samples equivalent to the first three seconds of samples 5 2 10 2 Input V indicator This field displays the actual voltage input 5 2 10 3 Temp C indicator This field displays the actual calculated temperature from the voltage input Page 32 of 55 L oLico 5 2 10 4 Lock LO button LOLIGOSYSTEMS COM This button can be used to copy the actual value from the Input V indicator to the LO input V field instead of manually reading and retyping the value 5 2 10 5 LO input V Use this field to enter the low input voltage from the Input V indicator during the low temperature calibration Please note The field is limited to values between OV to 5V 5 2 10 6 LO Temp C Use this field to enter the temperature during the low temperature calibration Please note The field is limite
43. n and file outputs Use moving average if you wish to smoothen the chamber oxygen Signal Please note No calibrated values will be displayed either on screen or in the data file before sufficient samples have been collected to compute the moving average E g if the moving average is set to 30 points and the sample rate is set to 10 Hz there will be no chamber oxygen values the first 30 samples equivalent to the first three seconds of samples 5 2 8 2 Input V indicator This field displays the actual voltage input 5 2 8 3 pO kPa indicator This field displays the actual calculated oxygen partial pressure from the voltage input 5 2 8 4 Lock LO button This button can be used to copy the actual value from the Input V indicator to the LO input V field instead of manually reading and retyping the value 5 2 8 5 LO input V Use this field to enter the low input voltage from the Input V indicator during the chamber oxygen calibration Please note The field is limited to values between OV to 5V 5 2 8 6 LO pO kPa Use this field to enter the oxygen pressure during the low level oxygen calibration of the chamber oxygen input Please note The field is limited to values between 0 00 kPa to 60 00 kPa 5 2 8 7 Lock HI button This button can be used to copy the actual value from the Input V indicator to the HI input V field instead of manually reading and retyping the value Page 30 of 55 L oLico 5
44. nment Page 23 of 55 Screen 23 DAQ 4 instrument FLUSH RECIRC O2 N2 TEMP 100 240VAC L oLico LOLIGOSYSTEMS COM A Respirometer Flush pump Recirc pump Screen 23 shows how to connect the flush and the recirc pump to the respirometer by tubes The connection to the DAQ 4 instrument dotted lines is described above Page 24 of 55 LOLIGOSYSTEMS COM 5 Using AutoResp 4 5 1 Startup e Click on the AutoResp 4 icon in the Start menu by following the folder hierarchy Shown on Screen 24 Screen 24 SI New Office Document LoliResp E NewShortcut2 Microsoft Access E Open Office Document Microsoft Excel Set Program Access and Defaults Microsoft FrontPage SW Windows Catalog Microsoft Outlook Ah Windows Update Microsoft PowerPoint SI WinZip Microsoft Word EH Accessories S Outlook Express Lan Acer ePM mi Acer GridVista Lan AcerSystem IEN Antivir PersonalEdition Classic EH C950 Multi Channel Gas Exchange Software Internet an CyberLink PowerDVD Internet Explorer 5 St Software e Remote Assistance Windows Media Player a Windows Movie Maker EH QuickTime Si Windows Live Messenger E WIBU KEY EN LoliTRACK gt Canon Utilities Ai E mail Games m EJ Microsoft Outlook an EN LABTECH NOTEBOOKpro Version 12 1 E Quattro Track fn Launch Manager EH Measurement Computing LoliTRACK EN Microsoft Office Tools EN National In
45. on Screen 13 AutoResp 4 Installation Readme Please read the following Information 2 WIBU KEY Setup i amme Operating system windows XP Source path Selected languages English The following components will be installed IWIBL KEY driver fles WIBL KEY COM control WIBLI S YSTEMS Shell Extension WIBLL EEY network server WIBL KEY tools Pot Back Install 17 When installation is done click Next again 18 Click Finish to end installation of the WiBu drivers LOLIGOSYSTEMS COM 19 Close all open programs and choose Yes I want to restart my computer now and click on Finish Screen 14 AutoResp 4 Installation Finished AutoResp 4 installation successful To finalize the installation your computer needs to be restarted Do wou want to restart your computer rnos es want to restart my computer now i Ho l will restart it later Click Finish to end the installation lt Back H je Cancel Page 12 of 55 L oLico LOLIGOSYSTEMS COM The following steps will explain how to finish the installation of the drivers together with your instruments 20 Connect the WiBu dongle to your computer via an USB port The dongle will be detected automatically 21 Connect the DAQ 4 instrument to the PC via the USB cable After a few seconds Screen 15 becomes present Screen 15 Found New Hardware Wizard Welcome to the Found New Har
46. on the Windows Update Web site with your pennission Can Windows connect to Windows Update to search for software Yes this time only O Yes now and every time connect a device No not this time Click Next to continue 25 Click on No not this time and then on Next Screen 19 Found New Hardware Wizard This wizard helps you install software for USB 6215 J if your hardware came with an installation CD lt or floppy disk insert it now What do you want the wizard to do Gei Install the software automatically Recommended O Install from a list or specific location Advanced Click Next to continue 26 Click on Install the software automatically Recommended and then on Next Page 15 of 55 Screen 20 27 Click Finish Screen 21 i E mail E osoft Outlook Quattro Track LoliTRACK Microsoft Excel of AutoResp 4 Measurement amp Auto lt Of FI Found New Hardware Wizard a New Office Document E NewShortcut2 a Open Office Document Set Program Access and Defaults W Windows Catalog Hi Windows Update D WinZip an Accessories ei Acer ePM Acer GridVista fl AcerSystem EN Antivir PersonalEdition Classic mi C950 Multi Channel Gas Exchange Software E CyberLink PowerDVD EN Eltima Software Lan Games e LABTECH NOTEBOOKpro Version 12 1 Launch Manager Measurement Computing EN Microsoft Office Tools La National Instruments
47. rmation about Loligo Systems Page 45 of 55 L oLico LOLIGOSYSTEMS COM 6 Troubleshooting 6 1 No connection to the DAQ 4 instrument Screen 32 Error 201003 occurred at DAQm Create Channel D0 Digital Qutput vi 1 Possible reason s Measurements Device cannot be accessed Possible Causes Device is no longer present in the system Device is not powered Device is powered but was temporarily without power Device is damaged Ensure the device is properly connected and powered Turn the computer off and on again IF vou suspect that The error shown on Screen 32 occurs when the NI USB 6215 DAQ card has no properly connection to the PC This error can occur in 2 ways 6 1 1 Name is not Devi To change the device name open Measurement amp Automation by following the folder hierarchy shown on Screen 21 page 16 The following example shows on Screen 33 a simulated device named as Dev1 The NI USB 6215 DAQ card is registered as Dev2 Page 46 of 55 X E Gf Properties X Delete Kn Self Test MA Test Panels fJ Reset Device init Create Task Gan Configure TEDS ei 3 Hide Help A o J Data Neighborhood Name Value Back fez Al 6 Devices and Interfaces ES serial Number 0x0 S a NI DAQmx Devices NI NI USB 6215 Dev2 4 i ec Basics PX PXI System Unidentified a a Scales a Software H E Remote Systems What do you want
48. se look into the DAQ PAC F4X package 8 6 OXY 4 Instruction manual The instruction manual for the OXY 4 fiber optic instrument from PreSens manual is included as an individual document Please use this manual for further information about the OXY 4 mini instrument and the OXY 4 software Please look into the DAQ PAC F4X package Page 55 of 55
49. sed the user is prompted to enter a file name and path see Screen 28 The path is by default the installation directory of AutoResp4 AutoResp4Data The current time and date xls is suggested as a filename in the prompt Both the path and filename can be altered by choice The system suggests two file extensions xls and txt The xls extension will create a tabulator separated text file which is readable by Microsoft Excel The txt extension will create a semicolon separated text file which is readable by any Microsoft Windows PC 5 2 11 2 Unit button Use this button to convert the displayed oxygen partial pressure values on the chamber oxygen graph into Torr oxygen saturation or air saturation 5 2 11 3 Play button Use this button to start stop data from being graphed When the play button is ON new data will be shown on the oxygen graph and the Unit button is enabled When the play button is OFF the Unit button is disabled Furthermore a scroll bar is shown for the chamber oxygen graph The state of the Play button also changes the menu of the Chamber oxygen graph see 5 2 11 page 34 Page 34 of 55 L oLico LOLIGOSYSTEMS COM 5 2 12 General about Screen 29 Screen 29 is the calibration screen as it looks if the chamber oxygen signal is acquired from the OXY 4 OXY 4 mini instrument and the RS232 port of the PC Please note When fiber optic is on as seen on Screen 29 the sample frequency cannot be altered as it is
50. struments Mi gt lt icrosoft Excel e NTI Backup NOW 4 ff NTI CD amp DVD Maker 7 AutoResp 4 a E Philips SPC 900NC PC Camera Le AutoResp4 gt l A r AutoResp 4 Location C Program Files AutoResp4 Windows Home Edition fn Pinnacle PCTV USB2 Paint EN REALTEK Gigabit and Fast Ethernet NIC Driver ei Startup e 1 E Vernier Software e Winzip CH Adobe Reader 7 0 geegent A Stait Internet Explorer DC e Er s WE V GE a a Er s NW SE 9 19 a 20 F g e If the USB hardware protection dongle is not connected to the PC an error dialog box pops up see Screen 25 Screen 25 cence e Connect the USB hardware protection dongle to the PC via an USB port and click on Retry Screen 26 now becomes present Page 25 of 55 Screen 26 LOLIGOSYSTEMS COM e When AutoResp 4 has started properly click on the File menu gt New experiment Screen 27 is now present Page 26 of 55 L oLico LOLIGOSYSTEMS COM 5 2 Calibration and selection of oxygen input 5 2 1 General about Screen 27 Screen 27 is the calibration screen as it looks if chamber oxygen input is a 0 5 VDC analog signal e g from an OXY REG or some other O instrument On this screen a common sample frequency for all inputs can be chosen and the inputs can be calibrated from voltages into oxygen partial pressures and temperature This screen can also be used to do a quick oxygen or temperat
51. t oO 100 Chamber 2 Resp vol Oi 211 0000 Wet weight oi e 100 Chamber 3 Resp vol l 211 0000 d Wet weight o 100 Chamber 4 Resp vol l 11 0000 Wet weight oi ST Oxygen control 20 50 aa Setpoint kPa d a Hysteresis kPa n 10 Temperature control L Setpoint C 115 00 Hysteresis C 0 10 Heat L oLico LOLIGOSYSTEMS COM ypoxic Hyperoxic Cool O C O ne A L _ Start experiment 5 3 2 File name button Use this button to change the path and filename of the data file and raw data folder The path specifies the location of the main data file and the folder containing raw data from the experiment The path is by default the installation directory of AutoResp4 AutoResp4Data The current time and date xls is suggested as a filename in the prompt Both the path and filename can be altered by choice The system suggests two file extensions xls and txt The xls extension will create a tabulator separated text file which is readable by Microsoft Excel The txt extension will create a semicolon separated text file which is readable by any Microsoft Windows PC In addition to the data file the system will also generate a folder named as the data file The dot in the filename will however be replaced by an underscore _ in the folder name In this folder fi
52. to do Run the NI DAQmx Test Panels ab Remove the device View or change device properties Ca D tg Stalt PY sceen 18 omp Pant screen 18 bmp Paint Q NL USB 6215 Devi Devi QOxmext i Myo or 9 53 AM Right click with mouse on the Devi and choose rename or press F2 Change the name to something different than Dev1 In this example the name is changed to devisimu see Screen 34 Screen 34 File Edit View Tools Help Configi ration E Gf Properties X Delete Self Test PR Test Panels QyResetDevice ini Create Task amp Configure TEDS d i Hide Help ER My System snide Nene KS EES Ss E Devices and Interfaces D Number Ox0 S NI DAQmx Devices NI WA NIUSB 6215 Dev isimu DAQmx Device i NI USB 6215 Devi Basics PXI PXI System Unidentified aE Scales D IS Software E Remote Systems What do you want to do ab Run the N EDAQmx Test Panels Remove the device View or change device properties el IL Lal j xma H Boho ty 9 53 AM Page 47 of 55 LOLIGOSYSTEMS COM l oLico LOLIGOSYSTEMS COM Now change the device name of the NI USB 6215 DAQ card to Dev1 The DAQ 4 instrument should now work properly with AutoResp 4 Screen 35 NI USB 6215 Devi Measurement amp Automation Explorer AX File Edit View Tools Help Configuration pg Properties X Delete b
53. ure measurement Please note The calibration values is not stored until an experiment has been started That means the calibration values are NOT stored when the Next gt button is pressed Screen 27 WA utoR sp 4 Help Sample frequency Barometic pressure hPa D sat kPa Fiber Optic OFF Set temp C cm me te 1Hz x l 1013 20 859 Cp 20 00 kPa Chamber oxygen 1 PS Chamber oxygen 2 Li Oxygen graph s 12 16 Mov Avg Input Di pO2 kPa Mov Avg Input Ni DO kPa Ca 15 66 2 z J 1 point l 2 92 12 23 1 point l 3 75 15 71 25 116 37 LO input v LO input OG LO pOe kPa om IA ri e ry Hi input i HI pO2 kPa HI input Vv HI pO kPa Lock HI op 5 00 20 96 Lock HI e Op A 20 96 Chamber oxygen 3 E Chamber oxygen 4 ei Mov Avg Input O pO kPa Mov Avg Input DI D kPa 1 point 3 92 16 42 tpoint vi 4 07 17 07 LO input V LO pOo kPa LO input O LO pOo kPa I ry V r Lock LO 0 00 10 00 Lock LO 10 00 10 00 Gei A a 2 HI input OI HI pO2 kPa HT input WA HI pO kPa Lock HI e op 20 96 Lock HI 5 00 20 96 a 4 A4 Ambient oxygen Is Temperature ba Mov Avg Input 4 DO kPa Mov Avg Input v Temp C 1 point gt l 3 16 13 27 1 point l 3 34 66 76 LO input LO pO2 kPa LO input v LO Temp C Lock LO r 0 00 J 0 00 Lock LO S o 00 J 0 00 HI input WA Hi pO2 kPa Hi input DI HI Temp C dabe 0 I Lock HI 5 00 s 120 96 Lock HI S 15 00
54. ys see Table 4 and the wiring diagram on Figur 1 Table 4 DAQ 4 connector NI USB 6215 pins Channel name linel Lo OLIGO LOLIGOSYSTEMS COM Through the Solid state PCB in the DAQ 4 instrument it is possible to connect up to 500 W equipment to every relay SPECIFICATIONS Specifications range 20 C to 60 C Common specifications Supply voltage universal 21 6 253 VAC 50 60 Hz or 19 2 300 VDC Internal consumption Max consumption Isolation voltage test operation Signal noise ratio Response time programmable Calibration temperature Accuracy Temperature Coefficient EMC immunity influence Potentiometer input min Potentiometer input max Relay outputs Relay function Hysteresis in display counts On and Off delay Sensor error detection Setpoint 0 3600 s Max voltage 250 VRMS Max current 2A AC Max AC power 500 VA Max current at 24 VDC 1A 3 2 W 3 5 W 2 3 KVAC 250 VAC Min 60 dB 0 100 kHz 0 4 60 s 20 28 C lt 0 1 of reading lt 0 01 of reading C lt 0 5 of reading 10 Q 100 kQ 0 1 25 1 2999 Make Break Hold Page 54 of 55 L oLico LOLIGOSYSTEMS COM 8 4 TMP REG manual The instruction manual for the TMP REG instrument is included as an individual document Please look into the DAQ PAC F4X package 8 5 OXY REG manual The instruction manual for the OXY REG instrument is included as an individual document Plea

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