MATRIX STANDARD KIT - Applied Biosystems
Contents
1. DNA sequencing or fragment analysis methods is conveyed expressly or by implication to the purchaser by the purchase of this product A license to use the DNA sequencing or fragment analysis methods for certain research and development activities accompanies the purchase of certain Applied Biosystems reagents when used in conjunction with an authorized DNA sequencing machine or is available from Applied Biosystems Further information on purchasing licenses to practice the DNA sequencing or fragment analysis methods may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA ABI PRISM Applied Biosystems MicroAmp and VIC are registered trademarks and AB Design Applera 6FAM Hi Di NED and ROX are 8 Dec 2004 trademarks of Applera Corporation or its subsidiaries in the U S and or certain other countries ey Copyright 2004 Applied Biosystems All rights reserved Product Insert Science VENDOR INFORMATION gt Applied PRODUCT INSERT BS Biosystems FULL SHEET Insert P N 4362883 REV A Printed in USA GUIDELINE INSERT FOLD INSTRUCTIONS Specification is intended for Full Sheet two sheets max Material 50 Offset White Dimensions Full Sheet 8 4 x 11 folded size 1 833 x 2 125 125 Print on one sheet front and back After final folding upper left hand corner of page 1 is to face out with title and part number displayed Folded inse2. MULTI CAPILLARY DS 31 DYE SET D wiVIC Applied MATRIX STANDARD KIT AS Biosystems Product P N 4345829 For Research Use Only Insert P N 4362883 REV A Not for use in diagnostic procedures Printed in USA The Matrix Standard Set DS 31 is used to generate the multicomponent matrix required when analyzing 6FAM VIC NED and ROX labeled DNA fragments on the Applied Biosystems 3130 and 3100 Series Systems and 3700 DNA Analyzer The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye labeled samples in a single capillary Matrix standards do not need to be run with every set of sample injections The standard only needs to be run once in order to generate a matrix file which is then applied to samples run under similar conditions For more information on the use of matrix standards refer to the instrument User s Manual or Getting Started Guide The kit consists of one tube of matrix standard which is sufficient for a minimum of eight array runs The Matrix Standard Set DS 31 contains specific sizes of DNA fragments labeled with a unique fluorescent dye label This standard is diluted in 1X TE buffer and is stable for one year when stored at 2 C to 8 C Do not freeze Preparing the Matrix Standard Set DS 31 for the Applied Biosystems 3130 and 3100 Series Systems WARNING CHEMICAL HAZARD Hi Di Formamide Exposure causes eye skin and r
3. e on the plate deck of the instrument 6 For specifics on setting up a run refer to your User s Manual or Getting Started Guide Preparing the Matrix Standard Set DS 31 for the ABI PRISM 3700 DNA Analyzer 1 Thaw and thoroughly mix the contents of the tube and spin briefly in a microcentrifuge 2 Prepare the matrix standard by combining 10 uL of standard with 390 uL of Hi Di Formamide P N 4311320 in a 1 5 mL microcentrifuge tube Mix thoroughly 3 Heat the mixture at 95 C for 5 minutes to denature and immediately place on ice 4 Divide the mixture into two 200 uL MicroAmp Reaction Tubes and place tubes at position 9 and 10 in the right 8 bar of the ABI PRISM 3700 DNA Analyzer plate deck 5 For specifics on setting up a run refer to the ABI PRISM 3700 DNA Analyzer User s Reference Manual NOTE If the signal height of any one of the matrix standard fragments is saturated on the above instruments reprepare the matrix standard mix with half the volume of matrix required in step 2 For example in step 2 for the 3130 and 3100 Series Systems with a 36 cm array reprepare the matrix standard mix with 5 uL of matrix standard and 195 uL of Hi Di Formamide P N 4311320 Rerun the spectral calibration Notice to Purchaser Disclaimer of License This product is optimized for use in the DNA sequencing or fragment analysis methods covered by patents owned or licensable by Applied Biosystems No license under these patents to use the
4. espiratory tract irritation It is a possible developmental and birth defect hazard Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves 1 Thoroughly mix the contents of the tube and spin briefly in a microcentrifuge 2 Prepare the matrix standard For a 36 cm array combine 10 uL of standard with 190 uL of Hi Di Formamide P N 4311320 in a 1 5 mL microcentrifuge tube For a 50 cm array combine 5 uL of standard with 195 uL of Hi Di Formamide P N 4311320 in a 1 5 mL microcentrifuge tube 3 Mix thoroughly and spin briefly in a microcentrifuge 4 Ifa GeneAmp 9600 9700 thermal cycler is available for denaturation follow steps A and B below A Dispense 10 uL of the matrix standard Hi Di formamide mixture into a 96 well microtiter plate For 16 capillaries dispense into two columns e g wells A1 H1 A2 H2 For 4 capillaries dispense into 4 wells e g wells A1 D1 B Cover the plate and denature at 95 C for 5 minutes Immediately place on ice Ifa GeneAmp 9600 9700 thermal cycler is not available follow steps C and D below C Heat the mixture at 95 C for 5 minutes to denature and immediately place on ice D Dispense 10 uL of the matrix standard Hi Di formamide mixture into a 96 well microtiter plate For 16 capillaries dispense into two columns e g wells A1 H1 A2 H2 For 4 capillaries dispense into 4 wells e g wells A1 D1 5 Place the 96 well microtiter plat
5. rts to be packaged as follows e Bundled in groups of 10ea e Shrink wrap 10ea groups for 100 inserts total e Label shrink wrapped bundles Part amp Rev font large enough to be legible Qty Manufacturer and Barcode Part 128C e Seal shrink wrapped bundles in an appropriately sized corrugated shipper not to exceed 25 or have outside dimensions greater than 12 x 13 4 x 24 e Appropriately label shipper Part amp Rev font large enough to be legible at 8 feet Description P O Qty Mfg Date Manufacturer Country of Origin and Barcode Part in 128C on at least one end panel 12 x 13 side On A won Company Logo Title of the Insert Up to 3 Lines of Information Product P N 00000000 Insert P N 00000000 Printed in Country NOTICE TO PRINTER Print page s prior to this page ONLY For Research Use Only ir use in diagnostic procedu This page is folding instructions which is NOT and MUST NOT BE PRINTED as part of this insert THIS DOCUMENT CONTAINS PROPRIETARY INFORMATION OF APPLIED BIOSYSTEMS AND IS TO BE RETAINED IN CONFIDENCE NOT TO BE REPRODUCED COPIED OR INCORPORATED IN ANY MANNER APPLIED BIOSYSTEMS 2004 1 Copyright 2002 Applied BiosystemsIAI rights reserved are registered trademarks and ahd are trademarks of Applera Corporation or it subsidi Product Insert other countries 1 DD Mon YYY 4343196 REV B
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