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1. 0 82min 40 Activities 31 Movements during the 4th 60sec period Animal was not in the White Zone during the 5th 60 sec period Animal was not in the White Zone during the 6th 60 sec period DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc CHAPTER 5 DATA ANALYSIS USING MED PC TO EXCEL Using a Pre Formatted Table Profile MTP file MED PC to Excel MPC2XL is a program that helps to import data from MED PC the raw data file format previous section to a spreadsheet program such as Microsoft Excel MPC2XL needs to be installed separately from MED PC Please refer to the User s Manual for MPC2XL for installation instructions Once MPC2XL is installed open the data folder The Place Preference MTP files have been automatically transferred from the CD to this file Follow the step by step instructions below for importing data 1 Open Microsoft Excel and then minimize the window to gain access to the MED PC to Excel icon on the desktop and double click on the icon The MED PC to Excel display see Figure 5 1 will appear The uppermost file display should be titled Transfer Data see the figure below Under the Table Transfer window at the bottom of the screen click on Select Figure 5 1 Table Transfer MED PC to Excel Bil x File Help Transfer Data Edit Row Profiles Edit Table Profiles MO PROFILE ASSIGNED Orientation Horizontal Rows Iraneten Vertical Columns WO PROFILE ASSIGNED Sie
2. MED ASSOCIATES INC W SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Array W holds the experiment values for what the animal did in the White Zone The first five elements in the array are the total counts for the entire Test default 15min The next five elements are the counts that happened in the first Bin default 60sec Exploration Entrance Zone Counts Time Counts 0 4 00 3 00 112 88 103 00 77 00 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 1 00 1 00 43 19 47 00 33 00 0 00 2 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 1 00 0 00 0 00 0 00 1 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 1 00 0 00 0 00 0 00 11 18 49 29 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 9 22 0 00 0 00 Activity Movement Counts 0 00 9 00 6 00 40 00 31 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 7 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 7 00 0 00 0 00 ae 4 Explorations 3 Entrances 112 88 60 1 88min 103 Activities 77 Movements Totals for the entire 15min 1 Exploration 1 Entrance 43 19 60 0 72min 47 Activities 33 Movements during the 1st 60sec period Animal was not in the White Zone during the 2nd 60 sec period 2 Explorations 1 Entrance 11 18 60 0 19min 9 Activities 6 Movements during the 3rd 60sec period O Explorations O Entrances 49 29 60
3. lt a Wranster 26 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 2 Choose the MTP file in the data folder that corresponds to the MED State Notation Procedure that was run and click Open For this example Place Pref Auto Doors mtp will be used Figure 5 2 Select File to Open Look in 73 Data Place Pref Auto Doors mtp Place Pref Man Doors mtp My Recent Place Pref Train 2 Animals mkp Documents Desktop hy Documents js hy Computer a File name My Network Files of type MED PC Table Frofile Cancel Pl aces Open as read only 3 Note that Place Pref Auto Doors MTP is listed under the Table Transfer Profile Select Labels and Data because selecting these options will print data labels as well as import data Click Transfer Figure 5 3 Transfer Data MED PC to Excel File Help Transfer Data Edit Row Profiles Edit Table Profiles Row Transfer Profile NO PROFILE ASSIGNED Select Transfer Orientation Column Labels f Horizontal Rows iv Data C Vertical Columns Table Transfer Profile CAMED PC IM Data Place Pref Auto Doors mtp Transfer iv Label maul Transfer iv Data 27 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 4 Specify the raw data file to transfer Raw dat
4. Bi496 Big Bi499 Bi499 Gray Expl Gray Ente Gray Zone Gray Acti GI Gu GZ Gi 5 5 5 5 G 1395 GASA GASA Gi456 White Exe White Ent White Zon White Act White ilo 1st Column Element AMI WT WZ WSJ VN Increment 5 5 5 5 5 Last Column Element WVi495 Vv 4Bb wv 43 voo Wida Protile in Editor CAMED PC Iv Data Place Pref Auto Doors mtp To edit either the Header Titles or Header Elements click on the appropriate cell in the Edit Table Profiles window Rows and columns can be added to the file First select the desired location then right click to add either the desired row or column Use the right click option titled Paste an Identifier to include subject or session identifying information Note that when using the Paste an Identifier function Header Titles and Header Elements are edited and pasted automatically To save the edited MTP file select Save and create a new filename in the data folder To use this newly edited and saved MTP file verify that the file is selected in the Table Transfer Profile display Figure 5 3 and then click Transfer 29 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc Appendix A Contact Information Please contact MED Associates Inc for information regarding any of our products Visit our website at www med associates com for contact information For technical questions email support med associates com 30 DOC 078 Rev 1 8 Copyright O 2012 MED Associate
5. Box Additional Bases to Update i DN Select All Deselect Al 21 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc CHAPTER 4 UNDERSTANDING THE DATA FILES Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data in is dependent on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC load wizard Figure 3 4 Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC to Excel might even be used Data file formats are explained in detail in the MED PC User s Manual Sample Data File The sample data file shown below is for Place Pref Man Doors mpc File C PROGRAM FILES MED ASSOCIATES MED PC DATA 2008 01 30 Start Date 01 30 08 Date that the program started End Date 01 30 08 Date that the program ended Subject Animal 1 Subject name Experiment Experiment 1 Experiment name Group Group 1 Group name Box 1 Box in MED PC that the program ran Start Time 9 35 33 Time that the program started End Time 10 43 06 Time that the program ended MSN Place Pref Man Doors Name
6. ENV 226B Connect the light in the white chamber to Channel 1 the light in the gray chamber to Channel 2 and the light in the black chamber to Channel 3 as shown below Repeat this step for each chamber 11 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 5 Using an SG 226B 25 cable connect the Power Operate connectors on the SG 226B associated with Chamber 1 to a 28 VDC power connector on the back of the SG 6080D cabinet SG 6080D 28 voLTs 5 i e H ANA B Fuse 8 Amp 250V MINI FB 5 me d associates ING SG 226B 25 CABLE ess Channel 1 Channel 2 Channel 3 E 6 Daisy chain each additional ENV 226B using an SG 226B 12 cable as shown below SG 6080D 28 VOLTS gt e POWER IN 110 Volts Fuse 8 Amp 250V MINI FB m eral E Med associates inc I SG 226B 12 op lt ee CABLE Tese Ieee Channel 1 Channel 2 Channel 3 e Channel 1 Channel 2 Channel 3 e CHAMBER 1 CHAMBER 2 12 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc 7 Apply power to the SG 6080D Interface Cabinet and the computer The wiring for the system is now complete Hardware Test Procedure Once the wiring is complete and power has been applied to the system the hardware can be tested using the MED Test software application If further information is necessary regarding the use of MED Test please refer to the MED Te
7. counts that happened in the first Bin default 60sec Movements are not recorded while the animal is in the Gray Zone Exploration Entrance Zone Activity Movement Counts Counts Time Counts Counts 13 00 6 00 107 13 74 00 0 00 13 Explorations 6 Entrances 107 13 60 1 79min 74 Activities O Movements Totals for the entire 15min 5 3 00 1 00 16 80 15 00 0 00 3 Explorations 1 Entrance 16 80 60 0 28min 15 Activities O Movements during the 1st 60sec period 10 0 00 0 00 11 60 8 00 0 00 0 Explorations O Entrances 11 60 60 25 40 45 50 55 60 65 70 75 0 19min 8 Activities 0 Movements during the 2nd 60sec period 15 3 00 1 00 16 81 8 00 0 00 3 Explorations 1 Entrance 16 81 60 0 28min 8 Activities O Movements during the 3rd 60sec period 20 1 00 1 00 10 71 8 00 0 00 1E xploration 1 Entrance 10 71 60 0 18min 8 Activities O Movements during the 4th 60sec period 0 00 0 00 10 28 5 00 0 00 0 Explorations O Entrances 10 28 60 0 17min 5 Activities O Movements during the 5th 60sec period 30 0 00 0 00 0 00 0 00 0 00 Animal was not in the Gray Zone during the 6th 60 sec period 35 0 00 0 00 0 00 0 00 0 00 1 00 0 00 0 00 0 00 0 00 2 00 1 00 22 18 20 00 0 00 0 00 0 00 0 00 0 00 0 00 1 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 2 00 2 00 18 75 10 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 24 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc
8. defined at the beginning of the experiment in the Named Variables DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION CHAPTER 2 HARDWARE Hardware Guide The hardware included with the Three Chamber Place Preference system will depend on whether it is equipped with automatic or manual guillotine doors as well as the number of chambers in the system All Systems Figure 2 1 ENV 256C 16 Channel IR Controller med associates inc E ENV 256C 16 CHANNEL IR CONTROLLER Receivers Transmitters OUTPUT Figure 2 2 3 Chamber Place Preference Chamber with ENV 256C Channel 1 Channel 2 Channel 3 amp DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Figure 2 4 Computer with DIG 704PCI Card Systems with Automatic Guillotine Doors Figure 2 5 SG 6510D Cabinet with DIG 700F DIG 721 DIG 709A and DIG 713A Cards Output Output Output Output Output o o Ev Super Super Super Pot Pote Pote d 3 Do oc zi d 5 2 z 0 09090000090 O 0 0 I n2 4 00000000 e09000000 Cu O da G3 MO 3 eo000000 09009000007 on Ow D wh 2 09000000 eoo000000 DO 9 U D D hn 3 KEKEL D 0609090900007 fO Cb U de Q3 MO a pL ve0000000 KIKIKI E nn ow D wh eo000000 eoo0000 eoc00000 eo
9. of the program that created this file C 1 00 Flag animal in maze when C 1 D 0 00 Not Used E 900 00 Elapsed Session Time in seconds Displayed as Decimal Minutes F 60 00 Elapsed Bin Time in seconds H 0 00 Not Used 75 00 Subscript for Data Time Bins J 0 00 Not Used K 0 00 Not Used L 0 00 Not Used M 14 00 Movement Flag N 0 00 Not Used O 0 00 Not Used P 0 00 Not Used Q 14 00 Movement Flag R 0 00 Not Used S 0 00 Not Used T 0 00 Not Used U 0 00 Not Used V 0 00 Not Used X 0 00 Not Used Y 0 00 Not Used Z 0 00 Not Used 22 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Array A holds the times that the program will run for the Acclimation and Test talso holds the size of the Bin in seconds The Bin is used for closer examination of what the animal did during each minute Defaults 5min 15min 60sec Acclimation Test Bin A Time Time Time 0 5 00 15 00 60 00 Array B holds the experiment values for what the animal did in the Black Zone The first five elements in the array are the total counts for the entire Test default 15min The next five elements are the counts that happened in the first Bin default 60sec Exploration Entrance Zone Activity Movement B Counts Counts Time Counts Counts 0 6 00 4 00 679 99 643 00 534 00 6 Explorations 4 E
10. 0 60 54 46 20 0 0 60 51 33 21 1 1 32 03 32 27 eS 0 60 66 57 23 60 01 80 70 24 25 Gray Exploration Counts Gray Entrance Counts Gray Zone Time Gray Activity Counts 26 13 6 107 13 74 ad 3 1 15 8 15 28 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc Editing the MTP file The MTP file can be edited to customize the transfer process and display the data of most interest See the User s Manual for MPC2XL for explicit instructions about how to modify the MTP file using the Edit Table Profiles screen see Figure 5 6 Header Titles are user defined and can include any information that will help label the data listed below the title Header Elements are the data points that will get transferred from the raw data file into Excel The raw data file will list the elements that can be included in the MTP file e g A Z Figure 5 6 Edit Table Profiles MED PC to Excel File Help Transfer Data Edit Row Profiles Edit Table Profiles Edit Made e 3 4 5 b Header Titles IEEE StartDate StartTime Experimer Group Box Comment Header Elements subject StartDate lStartTime Experimer Group Box Comment Header Titles Adaptatiar Test Time Bin Time Header Elements A A Al Column Titles 1st Calumn Element Increment Last Column Element Column Titles 1st Calumn Element Increment Last Column Element Column Titles Black Exe Black Ent Black Zon Black Act Black Moy Bi B B2 Bis B4 5 5 5 5 5 B 485
11. 000000 e000000 0000000000000 a o000000000000 cm c A Q T Med associates ino SG 21503 iit DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Systems with Manual Guillotine Doors Figure 2 7 SG 6080D Cabinet with DIG 700F and DIG 713A Cards Decode Super Super Super Super Super Super Super Port Port Port Port Port Input input P Input input p Input ut input A 1 ee 2 Uu e0000000 ec0000007 on tn Co h3 m eococc0000 eveo000000 Oo C 4 Wh e0c000000 eoeoo00000 on O amp Gh ec000000 aon U 4A Wh eo000000 KEKEKE vec000000 eeoccocoo n eveoe000000 ecoc00000 d __D d 9 d 9 s ug e gt gt DIG 700FpiG 713A DIG 713A DIG713A DIG 713A DIG 713A DIG 713A DIG 713A DIG 713A t 5 19 49 C t Wiring Instructions for System with Automatic Guillotine Doors Some of the wiring for the 3 Chamber Place Preference system will be completed prior to shipping Complete the remaining wiring by following these steps NOTE Be sure that all hardware is unplugged prior to completing any wiring 1 Using the SG 210CP 2 cable connect the DIG 700F decoder card to the 28 VDC power card as shown below Decode Output Output Output Sup
12. 1 Experiment PlacePreference 1 Group Group 1 Comment Place Preference Data Collection Filename PP Subjectl Groupl Click Next to finish loading the box or Previous to correct errors 17 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION The Alter Session Parameters Screen shown in Figure 3 6 is the next screen to appear and is an important screen for the researcher The Alter Session Parameters screen allows the researcher to alter the parameters by which a procedure executes The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration Figure 3 6 Alter Session Parameters Screen Adaption Time min Test Time min Bin Size sec In this example only 1 box is described in the Hardware Configuration so Figure 3 8 will appear next If more than 1 box is in the Hardware Configuration then Figure 3 7 will appear Figure 3 7 Send Start Command Screen for Multiple Box Configuration 18 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Figure 3 8 Send Start Command Screen for Single Box Configuration In both cases Figure 3 7 and Figure 3 8 the screens are where the researcher decides to either load more boxes send a start signal to boxes that are alrea
13. 3 Each SG 211F cable terminates at one end with a female 25 pin connector The other end terminates with eight tinned wires and two 080 pin connectors These cables are used to control and power the automatic guillotine doors The tinned wires connect to the DIG 721 output cards and the 080 pin connectors connect to the DIG 709A 28 VDC power distribution card The figure and table below illustrate the locations of each of these wires Output DIG 721 Output Gray amp Purple DIG 709A Distribution Pink amp Tan 00000000000 NO co lt o000000000000 c q Q y e gt 9 4 Connect the other end of the SG 211F cable to the male end of an SG 210A cable 5 Connect the female end of the SG 210A cable to the SG 215D3 that corresponds to the DIG 721 card it is connected to For example connect the card addressed as 1 to Chamber 1 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION N co lt 1 05 1 06 1 0 7 1 0 8 BEER 1 01 1 0 2 1 03 1 04 Medassociates ma SG 215D3 dj SG 211F CABLE SG 210A CABLE 6 Repeat Steps 2 5 for each additional DIG 721 output card 7 Using an SG 210TTL 20 cable connect each DIG 713A input card to the corresponding ENV 256C IR controller For example connect the DIG 713A card addressed as 1 to the ENV 256C IR contr
14. ACE PREFERENCE DATA COLLECTION Figure 1 1 Place Preference Chamber Zones and Beam Numbers For example if the animal is in the white zone refer to the White column in Table 1 1 The table indicates that any movement within that zone beams White1 White6 is considered Activity If the animal breaks the first beam in the gray zone Gray7 it is counted as Exploration If the animal breaks the second beam in the gray zone Gray8 then it is considered an Entrance Now that the animal has entered the gray zone refer to the Gray column Table 1 1 Reference Table Gray White Black White1 Entrance Activity Entrance White2 Entrance Activity Entrance White3 Entrance Activity Entrance White4 Entrance Activity Entrance White5 Entrance Activity Entrance White6 Exploration Activity Exploration Gray7 Activity Exploration Entrance Gray8 Activity Entrance Entrance Gray9 Activity Entrance Exploration Black10 Exploration Exploration Activity Black11 Entrance Entrance Activity Black12 Entrance Entrance Activity Black13 Entrance Entrance Activity Black14 Entrance Entrance Activity Black15 Entrance Entrance Activity Data File Arrays The data file will have arrays B G and W which correspond to the Black Gray and White zones Refer to Chapter 4 Understanding the Data Files for a sample data file The first row of each array contains the Totals for the entire session Each row that follows is the data for each time bin which is
15. a files are listed by date and then click Open This step performs the transfer and now the data has been sent to Microsoft Excel Figure 5 4 Specify Data Files to Transfer Specify Data Files to Transfer Look in Co Data er E3 E 2006 09 01 4 E 2006 09 05 My Recent 2006 09 07 Documents E 2006 09 13 s Place Pref Auto Doors mtp s Place Pref Man Doors mtp Desktop i Place Pref Train 2 Animals mtp JO My Documents 48 My Computer File name My Network Files of type Any File Cancel Places Open as read only 5 Expand the Microsoft Excel screen and the data should appear in the following format Figure 5 5 Excel Spreadsheet E3 Microsoft Excel Book ag File Edit View Insert Format Tools Data Window Help Adobe PDF Dc BGBl T SBA noA E A Sh ZI MB 100 g Arial 10 JBZU EZEBE S276 18 EEDA Al M Subject B C D E lel E E cH 1 I StartDate StartTime Experiment Group Box Comment 2 Animal 1 b 7 2007 9 35 33 Experiment 1 Group 1 1 E 4 Adaptation Time Test Time Bin Size 5 5 15 60 6 Black Exploration Counts Black Entrance Counts Black Zone Time Black Activity Counts Black Movement Counts ieee 6 4 679 99 643 534 9 0 10 3 1 48 4 43 36 11 0 0 32 01 27 22 12 0 13 1 1 49 72 46 38 14 60 42 17 15 60 61 52 16 60 60 54 17 1 1 37 82 22 23 18 60 59 59 ee 0
16. dy loaded or enter the MED PC run time environment without sending a start signal by selecting I am finished with the wizard This option results in the screen shown in Figure 3 9 Figure 3 9 The MED PC Run Time Screen MED PC IV File Configure View Macros Help alele sai amp elt el Box 1 Acclimation Time 5 00 Test Time 15 00 Bin Size 60 00 Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC run time screen Simply highlight the value to change and then enter the new value Once a session is in progress change variables by selecting Configure on the main menu and select Change Variables or click the 4th tool bar item BIX to reveal the window in Figure 3 10 In the lower left hand corner of the Change Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes 19 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Figure 3 10 Changing Variables Screen Displaying Variables from Box 1 Sera E array EUR BET Tm j Sahe m ELM cos An x i yars ias Won opos en cns l Refresh cs Cini tms Samo m Issue a Wu V Array jm Van Close
17. er Super Super Super Super Super ji P Port 4 Port Pot Port Port Port r i Ino nou 1 mE XP Td 96 cS 009900907 T 3 z 21 P2 o 20 3 e N d rR 0 o D cn A GW 8900000005 o 5 5 7 8e i DIG 713A DIG i 13 A 90090090 an o nod wr eeeeeee0 gt J 000000003 LEELEE EE O5 h3 eoococcc ono eon oO ho Why 006000000 st ee I e 00000900 a ae ceo o n2 TEISINA INTATT oo Nee ES LJ a Ld E L sd cem Ea P1 L o o e L4 e e o im a G2 C 0000000000000 K Is e 5 z J gt e 9 o o n DIG a WG 71 D SG 210CP 2 Cable 2 Using the SG 216B2 cable connect the 28 VDC power card to the DIG 709A 28 VDC power distribution card as shown below Red pin to red connector and black pin to black connector DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Output Output Output Output Output Output Output Super Super Super Port Port amp amp Port D redo p Indos s Ire eo 0 X j t nN 1 cw Au sew A 3 ee et 5 o5 o5 6 6 eeo0000 eeeseeeceo CO Ch Ch A WH o M v 00000900 e0000000 e0o000000 eo000000 3 ve0000000 eo000000 000000000000 DIG 713A DIG 713A c SG 216B2 Cable
18. icator lights up on the MED Test screen When this is complete click Clear Card and allow MED Test to run overnight When testing is complete there should not be any counts recorded on MED Test Be sure to test each Input Card by incrementing the Port Address NOTE The indicator lights on the MED Test screen and the LEDs on the DIG 713A card that correspond with P2 8 will remain on at all times as there is not an IR Beam associated with this input Figure 2 9 MED Test SuperPort Input Card Screen SuperPort Input DIG 12 713 Super Port Input P2 P1 Port Address 1 eo Offset Value 200 3 ee F1 Repeat Count 4 0O P2 Repeat Count 5 0 0 o eo 7 Q ON AT ALL TIMES 8 DIG 713A 14 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION CHAPTER 3 SOFTWARE Installation Please refer to the MED PC User s Manual for a complete guide to installing the MED PC software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility The MSN procedures utilized by MED PC are found by the application in a folder on the PC s hard drive Using the MED PC Load Wizard MED PC is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describe how to initiate an experiment based upo
19. le Values esesesseeeeeeeee nennen enne 19 Chapter 4 Understanding the Data Files eese 22 Samble Deta Filed uan a dede torte Ce de mittebat a denies 22 Chapter 5 Data Analysis Using MED PC to Excel 26 Using a Pre Formatted Table Profile MTP file 26 Fauna Mei RE E EU USUS 29 Appendix A Contact Information ccccccsssssccccccsssssecccccessssecccccesssecceceeeeeses 30 CHAPTER 1 INTRODUCTION Overview of the Procedures If the Place Preference Test chamber is equipped with auto guillotine doors then Place Pref Auto Doors mpc should be run All lights will be on when the procedure is loaded so that the user may make any necessary adjustment or perform testing When the start command is issued all lights are turned off Following the acclimation period the auto guillotine doors are opened and the lights turned on Doors are closed and lights turned off at the end of the session time If the Place Preference Test chamber is equipped with manual guillotine doors then Place Pref Man Doors mpc or Place Pref Train 2 Animals mpc should be run Both programs use the computer s speaker to signal the end of the acclimation and test periods The default duration for the audible alert is 5 seconds however the duration of the audible alarm may be modified or the alarm may be turned off using the menu item Shu
20. ly compiled procedures Simply select the procedure to be run and then click Next Figure 3 3 Select a Procedure Screen PLACE PREF AUTO DOORS PLACE PREF MAN DOORS PLACE PREF TRAIN 2 ANIMALS 16 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION The Enter Experiment Information Screen should display next as shown in Figure 3 4 The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and hit Next Figure 3 4 Enter Experiment Information Screen Subject_1 Subject PlacePreference 1 Experiment G roup Group_1 Comments Place Preference Data Collectior Optional Custom Filename PP_Subject _Group a J Close Previous The next screen to appear is the Review Choices screen as seen in Figure 3 5 This is a method of confirming that the information received from the Box Procedure Selected is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 3 5 Review Choices Screen Review the data below Box 1 Procedure PLACE PREF AUTO DOORS Subject Subject
21. m Help Display Data from Box Additional Boxes to Update C from Box 1 T reo 0 000 pO Select All Deselect Al The value of any simple variable may be viewed from this screen see Figure 3 11 Click an array on the table and each element in that array can be viewed To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 3 12 Change variables here as needed Figure 3 11 Displaying Array A from Box 1 Displaying Array G from Box 1 di Git Giz 3 G4 Named Wars 0 000 o 000 o 000 0 000 B Gl 8 G3 M arg D 000 o 000 0 000 0 000 i Grit G12 Gt G g 0 000 0 000 0 000 0 000 Refresh G16 G 17 Gite G8 0 000 0 000 0 000 0 000 i Issue G21 G22 G23 24 0 000 0 000 0 000 0 000 Close G2 27 G 28 i23 E 1 000 0 000 1 000 0 000 Help Display Data from Box Additional Boxes to Update GO from Box 1 A so 0 000 Ei bo Select All Deselect Al 20 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Figure 3 12 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Adaption Time min Named Wars Test Time min ME V Bin size sec zi Refresh zsue Clase Help nr Display Data from
22. m with Manual Guillotine Doors Some of the wiring for the 3 Chamber Place Preference system will be completed prior to shipping Complete the remaining wiring by following these steps NOTE Be sure that all hardware is unplugged prior to completing any wiring 1 Using the SG 210CP 2 cable connect the DIG 700F decoder card to any 28 VDC power connector on the back of the SG 6080D cabinet as shown below 1 1 zr Ws Fas SG 6080D 28 VOLTS Inout input 4 POWER IN 2 TAN 110 Volts Fuse 8 Amp 250V MINI FB n g 00000000 ALLEELE E ec000000 eeeeeeo 3 gt v00060000 7 ve00000007 Ing ASSOCIS ES ING SG 210CP 2 CABLE 2 Using an SG 210TTL 20 cable connect each DIG 713A input card to the corresponding ENV 256C IR controller For example connect the DIG 713A card addressed as 1 to the ENV 256C IR controller associated with Chamber 1 med associates inc E ENV 256C EJ Super 16 CHANNEL IR CONTROLLER Receivers Transmitters OUTPUT DIG 713A SG 210TTL 20 CABLE 10 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 3 Using the DIG 700C ribbon cable connect the DIG 700F decoder card to the DIG 704PCI card on the back of the computer 0 O _ gt F 3 Un lt 2 S raol DIG 700F 4 Connect the stimulus lights to the Lamp Output connectors on the
23. n the Place Pref Auto Doors mpc procedure The following steps that start this procedure will also apply to the Place Pref Manual Doors mpc and Place Pref Train 2 Animals mpc procedures Clicking either the MED PC icon from the Start Programs list or the desktop shortcut and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 3 1 will appear Figure 3 1 The MED PC Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 3 9 If the choice to continue with the Loading Wizard is made then select the Next button The next screen seen is the Box Selection screen as shown in Figure 3 2 It is in this screen that the 15 DOC 078 Rev 1 8 Copyright 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Figure 3 2 The Box Selection Screen The next screen seen is the Select a Procedure screen as seen in Figure 3 3 This is where the procedure to be run is made The screen displays a list of all the current
24. ntrances 679 99 60 11 33min 643 Activities 534 Movements Totals for the entire 15min 5 0 00 0 00 0 00 0 00 0 00 Animal was not in the Black Zone during the 1st 60 sec period 10 3 00 1 00 48 40 43 00 36 00 3 Explorations 1 Entrance 48 40 60 0 81min 43 Activities 36 Movements during the 2nd 60sec period 15 0 00 0 00 32 01 27 00 22 00 0 Explorations O Entrances 32 01 60 0 53min 27 Activities 22 Movements during the 3rd 60sec period 20 0 00 0 00 0 00 0 00 0 00 Animal was not in the Black Zone during the 4th 60 sec period 25 1 00 1 00 49 72 46 00 38 00 1 Exploration 1 Entrance 49 72 60 0 83min 46 Activities 38 Movements during the 5th 60sec period 30 0 00 0 00 60 00 42 00 17 00 0 Explorations O Entrances 60 60 1min 42 Activities 17 Movements during the 6th 60sec period 35 0 00 0 00 60 00 61 00 52 00 40 0 00 0 00 60 00 60 00 54 00 45 1 00 1 00 37 82 22 00 23 00 50 0 00 0 00 60 00 59 00 59 00 55 0 00 0 00 60 00 54 00 46 00 60 0 00 0 00 60 00 51 00 33 00 65 1 00 1 00 32 03 32 00 27 00 70 0 00 0 00 60 00 66 00 57 00 75 0 00 0 00 60 01 80 00 70 00 223 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 0 Array G holds the experiment values for what the animal did in the Gray Zone The first five elements in the array are the total counts for the entire Test default 15min The next five elements are the
25. oller associated with Chamber 1 med associales inc b ENV 256C Super 16 CHANNEL IR CONTROLLER Port Input P2P1 100 2 00 3 eoe 400 5 ee 6 ee 7 ee 8 Receivers Transmitters EHHEHH EHHHHE lt gt m OUTPUT SG 210TTL 20 CABLE 8 Using the DIG 700C ribbon cable connect the DIG 700F decoder card to the DIG 704PCI card on the back of the computer DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION 9 Connect the stimulus lights to the Lamp Output connectors on the ENV 226B Connect the light in the white chamber to Channel 1 the light in the gray chamber to Channel 2 and the light in the black chamber to Channel 3 as shown below 10 Using the included SG 216A cables connect the Power Operate connectors on the ENV 226B to the SG 215D3 I O connectors 3 5 as shown below DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Channel 1 Channel 2 Channel 3 11 Connect the auto doors to the SG 215D3 I O connectors as shown below T 1 05 1 06 1 07 1 08 BERE 12 Apply power to the SG 6510D Interface Cabinet and the computer The wiring for the system is now complete DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Wiring Instructions for Syste
26. s Inc
27. se Img d JSSOCIA ES ING instrumentation and software for research PLACE PREFERENCE DATA COLLECTION SOF 700RA 4 MED STATE NOTATION PROCEDURE DOC 078 Rev 1 8 Copyright 2012 All Rights Reserved Med Associates Inc P O Box 319 St Albans Vermont 05478 Phone 802 527 2343 Fax 802 527 5095 www med associates com SOF 700RA 4 CPP SOF 700RA 4 CPP Table of Contents Chapter 1 INTrOCUCTION ccccccssssssccccccsssscecccccessscecccceesseccccecassseeeceeceesseeseesees 1 Overview or the PFOCedul 85 riter Ip ERA UEFA NM EM n UN PREV 1 Definitions of Data ValUle5 stem ideft a tom petet em de aedem dn 1 Bye ea el ete An ene eer mm 2 Chapter 2 HardWare eenaa A eee 3 Hardware GUE eR E 3 Systems with Automatic Guillotine DOors eseesseeeeeeennnnnnn nnn 4 Systems with Manual Guillotine Doors esssseeeeeeeeeennnn nnne 5 Wiring Instructions for System with Automatic Guillotine Doors 5 Wiring Instructions for System with Manual Guillotine Doors 10 Hardware Pest PFOCeGlE8 ie noo uei tS attese IU ea co nsum te bte lese tuo Caso dt mop utu ge runde 13 Chapter 3 SOfDBWOFe uoi crei ate n iessbM vid ea Co MR E ES MEME OS ENDE 15 MAST lleno eer Tc T TS 15 Using tbe MED PC Logd MIZGaEG uit Coa bet ue eps idi eb uten ol ub Euer eu E e E DELE 15 Viewing Changing Variab
28. st User s Manual If the system fails any portion of these tests verify that the system is wired properly and run the test again If the failure persists it should be reported to MED Associates Technical Support Output Card Lights Auto Doors From the MED Test main screen select Standard Card Output Card DIG 720 721 722 and the screen shown below will appear If the system is equipped with Automatic Guillotine Doors activating Output 1 should raise Door 1 and Output 2 should raise Door 2 Activating Output 3 should turn on the stimulus light in the white chamber Output 4 should turn on the stimulus light in the gray chamber and Output 5 should turn on the stimulus light in the black chamber When Outputs 3 5 are activated adjust each Lamp Intensity knob on the ENV 226B to verify that the light intensity can be adjusted Be sure to test each Output Card by incrementing the Port Address Figure 2 8 MED Test Standard Output Card Screen Standard Output Card DIG 720 721 727 Port Address 780 Offset salue Toggle Bits AutoTest Clear Output Exit 13 DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc MED ASSOCIATES INC SOF 700RA 4 PLACE PREFERENCE DATA COLLECTION Input Card IR Beams From the MED Test main screen select SuperPort Input Card DIG 712 713 and the screen shown below will appear Break each of the IR Beams and verify that the corresponding ind
29. t Off Beep under the Configure menu If Place Pref Train 2 Animals is run then one animal should be placed in the Black Zone and the other in the White Zone prior to starting the program Select a Place Preference procedure and the Load Wizard presents a detailed list of session parameters From this window the user may set the adaptation time test time and bin size The default settings shown make the adaptation time 5 minutes the session time 15 minutes and the bin size 60 seconds Definitions of Data Values The Place Preference Data Collection procedures generate data files that contain values for Activity Movement Exploration Entrance and Zone Time Activity Any beam break within the current zone Movement A change in the beam broken in the current zone Exploration Breaking of the first beam in a zone Entrance Breaking of any beam beyond the first in a zone Zone Time The amount of time in seconds spent in the zone Figure 1 1 illustrates the three different zones as well as the beam numbers Table 1 1 helps to explain Entrance Exploration and Activity The leftmost column contains all of the beams on the Place Preference chamber The next three columns represent the three different zones in the Place Preference chamber Wherever the animal is in the Place Preference Chamber refer to the corresponding column DOC 078 Rev 1 8 Copyright O 2012 MED Associates Inc l SOF 700RA 4 PL

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