Biomek NX Multichannel Quick-Start Guide - QB3
Contents
1. Method1 BiomekXMC BiomekNX MC ETC 0 00 02 Figure 2 3 Instrument Setup step with labware added Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 6 Naming Labware It is helpful to name labware particularly when you use lots of labware on the deck You can assign a name that identifies the contents of the labware or a descriptive name that fits the work being done in the laboratory Beckman Coulter Inc 3 14 15 Learning to Create Methods for the Biomek NX From the Deck Layout Display double click the reservoir on P4 to display Labware Properties Figure 2 4 tesy Labware Type Reservoir Maximum Yolume 110000 uL Bar Code E Labware contains a known x volume 100000 a uL of liquid type MERS Sense the liquid level the first time a well with Unknown or Nominal volume is accessed from the Liquid Sense the liquid level every time a well is accessed from the Liquid Show Labware Volumes OK Cancel Figure 2 4 Labware Properties for Reservoir on P4 In Name enter Resv in Labware Properties In Labware contains a select Known from the pull down menu In volume enter 100000 This means that you know you have 100 000 microliters of liquid in the reservoir In liquid type select Water from the pull down menu Labware Properties for the Reservoir should look like Figure 2 4 Choose OK to close La2. Method1 BiomekNX MC BiomekNX MC ETC 0 00 03 Figure 2 7 Transfer step inserted into the Method View Beckman Coulter Inc ring to Create Methods for the Biomek NX 2 9 2 4 2 Configuring Tip Handling Tip Handling Configuring Tip To configure tip handling Handling is not always 1 In Tip Handling make sure Load is checked Figure 2 7 required because the BONE ENANA ESA 2 Click on the AP96_200uL tip box on TL1 in the Current Deck Display to load AP96_200uL tips as part of the Transfer step This change is displayed in the loads clean tips i i Load field of the Tip Handling configuration automatically during a Transfer step Refer to the Biomek Software User s Manual Chapter 15 3 1 Configuring Tip Handling for ae information on other tip Your tips are configured for your liquid transfer so click the up arrow next to handling options such as Tip Handling to collapse the configuration This allows more room for the step Wash tips in and configuration Figure 2 8 The text displayed reflects your tip handling Change tips between configuration destinations Make sure unload them is selected in the next field Check Change tips between sources Uncheck Change tips between destinations nH wn A U Biomek Software Method1 New File Edit Project Instrument Execution Options Help De eB RS eM oe aly wo B Start Use pod Pod1 v for transfer ARA a
3. 23 2004 2 16 00 PM Figure 2 17 Deck confirmation prompt for Quick Start method Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 18 Learning to Create Methods for the Biomek NX INGA 5 Choose OK The prompt closes and the method continues View the method on screen as it proceeds in the simulator The method can also be followed in the Method View as steps are highlighted in green as they are executed Note If you are running this method on your instrument instead of the simulator make sure that the physical deck layout matches the expected deck layout shown in the deck confirmation prompt before you choose OK You can proceed directly to Chapter 3 Using the Editors While Creating Methods to learn about the editors you ll use for customizing your methods Beckman Coulter Inc Using the Editors While Creating Methods 3 1 Overview In this chapter you will be given information and instructions for using the editors in the Biomek Software The editors allow you to customize a method to your special requirements You can go beyond the predefined project items and instrument configurations manually control the pod restore instrument settings and project files and configure external devices using specific editors The editors are accessed from the Project or Instrument menus located on the toolbar of the Biomek main editor Figure 3 1 Read the information in thi
4. COULTER Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide Beckman Coulter PN 987893 Revision AB September 2004 B ule Coulter Inc 4300 N Harbor Boulevard Fulle rton CA sae 3100 Copyright 2004 Bec invited ulter Inc Printed in U S A Except as provided in writing signed by an officer to Beckman Coulter Inc this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but Beckman Coulter Inc does not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability currentness omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user Except as expressly provided herein Beckman Coulter Inc makes no other warranty whether oral or written expressed or implied as to any matter whatsoever including but not limited to those concerning merchantability and fitness for a particular purpose nor is freedom from any patent owned by Beckman Coulter Inc or by others to be inferred LIMITATIONS OF LIABILITY Beckman Coulter Inc shall not be liable to any extent whatsoever for any damages resulting from or arising out of the use or performance of this sy
5. Overrides Technique wa amp Destination Dest1 JECFIat96 x at Dest x 100 uL of Water x JV Auto Select Customize Technique Standard ve ay amp Stop when finished with destinations Dispense up to 1 time per draw Transfer Detai Is collapsed Method1 BiomekNx MC BiomekNxX MC ETC 0 00 03 Figure 2 10 Transfer step with Destination configured Note The method view changes to reflect the amount transferred when the next step is configured The Transfer step configuration is now complete Proceed to the next section to configure a Move Labware step in your method Beckman Coulter Inc fing to Create Methods for the Biomek NX 2 13 2 5 Configuring a Move Labware Step The Move Labware step uses the gripper to move Gripper labware from one position to another on the deck or from Th has t one ALP to another Move Labware can also be used to S EEPE Tas WS mechanical fingers that remove labware from the deck or to an external hardware df he vod device without halting the Biomek instrument or the exten rom the po to method grip labware Gripper fingers grasp labware Note Refer to the Biomek Software User s Manual along the long side Chapter 15 4 Move Labware Step Refer to the Biomek NX Multichannel To configure a Move Labware step to move your Dest1 Laboratory Automation labware at position P5 to another deck position Workstation User 5 1 Inser
6. Figure 3 7 Deck Editor 2 Select New Deck Choose a name for this deck appears Figure 3 8 Choose a name for this deck Please enter a name em Figure 3 8 Choose a name for this deck 3 Enter QuickStartDeck and choose OK QuickStartDeck appears 4 Click and hold the mouse button on TrashRight from the ALP Types List Locations capable of supporting that ALP are indicted in the Deck View by dashed boxes Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 10 Using the Editors While Creating Methods 5 Drag and drop TrashRight to any location capable of supporting it Figure 3 9 A TrashRight ALP has been added to the deck This deck is now the default deck and is available in the Instrument Setup step yo x E fF al al Pp NewDeck Delete Deck Rename Deck OpenDeck Clear Deck Renumber Delete ALP Properties Save TrashRight ALP added to the deck Coords cm 0 303 13 436 fis 935 A Figure 3 9 QuickStartDeck with TrashRight added 6 Select Open Deck Select a Deck appears Figure 3 10 Select a Deck Figure 3 10 Select a Deck 7 Choose Deck1 to make it the default deck since it is the deck you used when you created the quick start method and choose OK 8 Choose Save to exit the Deck Editor Beckman Coulter Inc Using the Editors While Creating Methods 3 11 3 6 Manually Controlling the Pod If you
7. HEE eR AS 3 9 3 6 Manually Controlling the Pod nnannananaa nanana 3 11 3 7 Quick Start Guide Summary ooon nannan enna 3 13 Beckman Coulter Inc Introducing the Biomek NX Laboratory Automation Workstation 1 1 Introduction Welcome to the Biomek NX Laboratory Automation Workstation This quick start guide is designed to introduce you to the Biomek NX Multichannel instrument and Biomek Software operating system This quick start guide includes Getting to Know the Biomek NX Laboratory Automation Workstation this chapter an overview of the hardware and operating software Learning to Create Methods for the Biomek NX Chapter 2 an overview of how to create methods using the Biomek Software e Using the Editors While Creating Methods Chapter 3 an overview of how to use the editors in Biomek Software to accommodate unique method requirements Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 1 2 Beckman Coulter Inc Introducing the Biomek NX Laboratory Autome 1 1 1 How to Use This Quick Start Guide This quick start guide is intended to help you start using the Biomek NX instrument and Biomek Software immediately Read through this first chapter to acquaint yourself with the system proceed to Chapter 2 and complete the tutorial steps for building a small method then use Chapter 3 to complete the tutorial steps for using the edito
8. ror in the Dispense operation of the Defa Template pipetting template Cannot move relative to liquid leveli K Method1 Biomeknx Mc BiomekNx MC Error trying to dispense to P5 coming from P4 Error in the Dispense operation of the Default Beckman Coulter Inc Figure 2 12 Quick Start method with error 2 7 Methods and Project Files Methods are saved as elements of a project file not as separate method files Saving a method saves only the current status of the method It does not create a revision or check in project items as revisions Refer to the Biomek Software Users Manual Chapter 6 Understanding and Using Project Files ling to Create Methods for the Biomek NX Saving a Method After you ve clicked on Finish step you can save or check in your method You will just save it for now 1 Choose File gt Save from the toolbar Save Method AS appears Save Method As Enter method name Cancel Figure 2 13 Save Method As 2 Enter Quick Start Method and choose OK The method editor now looks like Figure 2 14 Biomek Software Quick Start Method Development x File Edit Project Instrument Execution Options Help PLAC ORS YBBoo gl wo K B Start Instrument E Instrument Setup Q Transfer 100 pL frc Move Labware fror od lg Finish Setup Comment Vv Clear current instrument setup of all labware after the method completes Vv
9. Nme o fj AccuFrame 8 Podi Port simulate bat Devices A DeviceControllerO Pod Type span Pod x g7 ShakerALPO oF ShakerALP1 oF StirrerALPO oF StirrerALP1 47 TiploaderO 67 Tiploader1 Digital Devices B E Simulator OP Camera E Materials f Stacker Carousels BiomekNx Span Figure 2 15 Hardware Setup 2 In Port select Simulate 3 Choose Accept to save changes and close Hardware Setup Biomek Software is now running in simulation mode Beckman Coulter Inc Learning to Create Methods for the Biomek NX 2 17 4 Click on the green arrow button on the toolbar The simulation display is automatically launched and the step configuration area is replaced with a three dimensional model of your Biomek NX Figure 2 16 The deck confirmation prompt Figure 2 17 appears on top of the main editor Biomek Software Quick Start Method Development File Edit Project Instrument Execution Options Help Dee BRS BRB oo 8 k Instrument Instrument Setup a Transfer 100 L fre Move Labware frorn IB Finish ft 2l l Quick Start Method BiomekNX MC BiomekNx MC ETC 0 00 00 Figure 2 16 Biomek NX simulator displayed Biomek Software The left pod should have no tips loaded Does the Biomek Software deck match the above layout including the labware and their locations If yes choose OK to continue the method If no choose Abort to stop the method
10. Tip and Labware Types e Technique Browser allows you to customize techniques for your specific needs and save them for use in future methods refer to the Biomek Software Users Manual Chapter 9 Understanding and Creating Techniques e Liquid Type Editor allows you to create new liquid types or to modify existing liquid types refer to the Biomek Software User s Manual Chapter 8 Understanding and Creating Liquid Types e Pipetting Template Editor allows you to create or modify pipetting templates that configure the precise actions during pipetting operations refer to the Biomek Software Users Manual Chapter 10 Using the Pipetting Template Editor 3 2 2 Instrument Menu The editors on the Instrument menu Figure 3 1 allow instrument configuration to be changed They include the following e Hardware Setup if you change the configuration of your instrument it is used to install configure and remove other devices refer to the Biomek NX Multichannel Laboratory Automation Workstation User s Manual Chapter 3 1 1 Accessing Hardware Setup e Deck Editor used to define and change deck configurations refer to the Biomek Software User 5 Manual Chapter 5 Preparing and Managing the Deck Note Refer to Section 3 5 Creating a New Deck in this quick start guide to follow step by step instructions to create a new deck layout in the software e Device Editor external devices may
11. Volume field Note The volume is measured in microliters uL automatically and indicates the amount of liquid that will be dispensed 100 uL into each of the 96 wells so in this case you re dispensing a total of 9600 uL Right click on the large tip illustration and choose Measure from Bottom Set the dispense height in the large tip illustration to 1 00 mm from bottom using the same technique as you used for setting the aspirate height Note If you accidentally open too many destination configurations right click on the title above the graphic of the plate in the configuration Click on Delete from the popup menu and the entire configuration goes away Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 12 Learning to Create Methods for the Biomek N The destination labware is now configured and the main editor looks like Figure 2 10 The Transfer step is now configured to transfer 100 uL from the source reservoir Resv to the destination microplate Dest1 Biomek Software Method1 New Fie Edit Project Instrument Execution Options Help PEALE ELI CETACEI Start Instrument Setup Q Transfer 100 uL frc B Finish Changes to reflect the amount transferred when the next step is configured Use pod Pod1 w for transfer V Load AP96_200uL tips change between sources and unload them when finished 1 00 mm from bottom
12. about the possibilities designed into the Biomek NX In this chapter you ll learn Creating a New Method Section 2 2 e Configuring the Instrument Setup Step Section 2 3 Configuring a Transfer Step Section 2 4 e Configuring a Move Labware Step Section 2 5 e Using the Finish Step Section 2 6 e Saving a Method Section 2 7 e Running a Method Section 2 8 Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 2 2 2 If using Accounts amp Permissions You must have Develop Methods permission to create methods Refer to the Biomek Software User 5 Manual Chapter 2 Using Accounts amp Permissions Learning to Create Methods for the Biomek Creating a New Method In this chapter you will build a simple method that transfers liquid between labware In building this method you will e Populate the deck with labware using an Instrument Setup step e Transfer liquid using a Transfer step This requires that a Tip Loader ALP be installed on the deck e Move labware via the gripper using a Move Labware step Tip Loader ALP The Tip Loader ALP loads disposable tips onto a 96 well head or a 384 well head mounted on a Multichannel Pod Refer to the ALPs User s Manual Chapter 23 Tip Loader ALP You launched Biomek Software in Chapter 1 you are now going to create a small method 1 Drag and drop an Inst
13. and approved by an expressly authorized officer of the Company Parts replaced during the warranty period are warranted to the end of the instrument warranty Note Performance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide Table of Contents Warranty and Returned Goods Requirements 00 cece cece eens iii Table of Contents 00 cee eee ee ee eee v 1 introducing the Biomek NX Laboratory Automation Workstation 1 1 TOU NOHO i 2 3 6 605 aaue eera ened aaa a a aes 1 1 1 1 1 How to Use This Quick Start Guide 0 0 0 0 ccs 1 2 1 2 Bi mek INK pc ee cca ae aos KAA RE RA eta RSA ARAM EEEE EREEREER EEA 1 3 1 2 1 Automated Labware Positioners ALPs 0 000 cee eeae 1 4 1 2 2 Light Curtain and Other Protective Barriers 004 1 5 1 3 Biome k SOUWAC 24 ccvesadenctusedenn Ce ei oies i ead hua taneaa eee eden 1 6 1 4 Starting the Biomek NX win ee sadeea seen aba eked peed desu ead eee ns 1 7 1 4 1 Turning On the Biomek NX i dacecaeiadaunad pea daundGes beens 1 7 1 4 2 Launching the Software 2444 4 Ses Ss aa wean dh heed dn beees 1 7 1 4 3 Homing All Axes of the Biomek NX Pod 00 000 1 8 2 Learning to Create Methods for the Biomek NX 2 1 OVETVIEW aitia RiGee hha ag Geeks eRe a a e ae e 2 1 2 2 Creating a New Method 00 cnc ce
14. be integrated to your Biomek NX In order to use the integrated device it must be configured through the Device Editor refer to the Biomek Software User s Manual Chapter 22 4 1 Configuring Devices Using the Device Editor e Manual Control allows you to control the movement of the bridge head and gripper independently of a method It is used to control the pod when framing the deck or gripper and when recovering from errors refer to the Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 4 Using the Editors While Creating Methods Biomek NX Multichannel Laboratory Automation Workstation User 5 Manual Chapter 5 Manually Controlling the Biomek NX in Biomek Software Note Refer to Section 3 6 Manually Controlling the Pod in this quick start guide to learn how to manually move a pod Import Export Utility allows you to import and export instrument settings decks framing tools and pod settings as import files imp refer to the Biomek Software User s Manual Chapter 3 Using Instrument Files and Settings Beckman Coulter Inc Using the Editors While Creating Methods 3 5 3 3 Creating New Labware in the Labware Type Editor The Labware Type Editor is used to create labware definitions so you can use labware that is not predefined in Biomek Software Labware Definitions Assume that you have new labware that is very similar to a BCFlat96 but some
15. labware during operation A Beckman Coulter Service Engineer frames the instrument during installation but it may be necessary to repeat the framing procedure if ALPs or devices are added moved or removed from the deck or if the head on the pod is changed Refer to the Biomek NX Multichannel Laboratory Automation Workstation User 5 Manual Appendix 4 Framing Using the Editors While Creating Methods 3 9 Creating a New Deck A deck in Biomek Software refers to the software representation of the physical Biomek NX deck The Deck Editor is used to define and change deck configurations and stores deck information used for multiple methods A deck also stores framing information and device associations for each position on the deck Note Refer to the Biomek Software User s Manual Chapter 5 Preparing and Managing the Deck Assume you have added a Trash ALP to your instrument deck and now must create another deck in the Deck Editor that reflects that addition to the physical deck To create a new deck in the Deck Editor 1 Choose Instrument gt Deck Editor Deck Editor appears Figure 3 7 x t E my NewDeck Delete Deck Rename Deck OpenDeck ClearDeck Renumber Delete ALP Properties Save Cancel BarcodeReaderRight FourByThree MagBeadALP OneByFive OneByOne OneByThree OrbitalShakerALP PositivePosition amp LP Coords cm fo s03 13 436 fis 935
16. need to manually control the Biomek NX instrument outside of a method including moving the bridge head and gripper you II use the Manual Control editor Manual Control is also used to control the pod when framing the deck or gripper and when recovering from errors Refer to the Biomek NX Multichannel Laboratory Automation Workstation User 5 Manual Chapter 5 Manually Controlling the Biomek NX in Biomek Software for additional information Note Manual Control is used also for controlling individual ALPs and Device Controller outside a method refer to the ALPs User 5 Manual Using the following instructions you ll use Manual Control to move the pod to a different position on the deck You ll move the pod to the Z max height first to make sure you don t hit anything on the deck and then you ll move the pod to a position To move the pod using Manual Control 1 Choose Instrument gt Manual Control Manual Control appears Figure 3 11 Here is where you choose the position to which you want to move the pod s a tt Advanced Controls Home All Axes Get Version Stop Exit Click on a position to move the pod to it Figure 3 11 Manual Control Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 12 Using the Editors While Creating Methods 2 Select Advanced Controls and highlight Pod1 Advanced Manual Control Pod1 appears Figure 3 12 Advanced Manua
17. round flat bottomed wells Preview The shape and general description of the There are no errors in this labware type SHSSSSSSSESE labware definition Figure 3 5 NewLabwareQuickStart Note Each piece of labware in Labware Types has its own mini editor similar to Figure 3 5 to allow specifications to be revised and saved 5 In Height enter 1 46 and choose Save You have created a new piece of labware in the Labware Type Editor 6 Choose Exit to close the Labware Type Editor Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 8 3 4 Revisions Changes made to any item in a project file are saved but do not become revisions until checked in Once checked in a new revision is created for the project items Revisions can never be lost If a project item is later modified or deleted any revision of the project item may be restored Validated Methods When Accounts amp Permissions is enabled methods may be validated A validated method is a revision of a method that is checked in approved with an electronic signature and protected from further modification Revisions of project items required to run the validated method are also checked in and protected from further modification This ensures that validated method runs are reproducible Refer to the Biomek Software User s Manual Chapter 12 15 Checking Out a Method Beckman Coulter Inc Using the Ed
18. tell the software what labware and ALPs are on the Avoidance deck for obstacle avoidance see sidebar You ll learn more about the Deck Editor in When building a method the next chapter refer to Section 3 5 Creating a New Deck you must ALWAYS use the Deck Editor and Instrument Setup step to tell the software what labware and ALPs are on the deck and the position each occupies If this is not done the pod could collide into these objects as it moves around the deck during a method run Biomek Software Method1 New File Edit Project Instrument Execution Options Help D ee BRS seeBlor alr no x B Start Deck Deckt x JV Pause to confirm setup Pause for bar code input Serena Instrument Setup Labware Category lt Any gt 7 IV verify Pod Setup Configure 7 BGS Setup a AB84M Ap384 3C AP96_20C AP96_20C AP36_20 AP96_20 BCDeep BCDeep9 BCFIatIE BCFulRe OSeBEa80 z CostarCe CostaFl CostarFl i il Greineri Greinerit p Collar_36r CollarSpa CostarDer Drainable FilterHole il iner ET BCSPECol Comment Method1 BiomekWXMC BiomekNX MC ETC 0 00 02 Figure 2 2 Instrument Setup step in the Method View Beckman Coulter Inc Learning to Create Methods for the Biomek NX 2 5 To configure the Instrument Setup step position TL 1 In Deck make sure the default deck layout Deck1 is selected The Instrument Setup s
19. 2 1 Automated Labware Positioners ALPs and for integrating additional devices such as detectors and washers A gripper is installed on the Multichannel Pod for grasping and moving labware Note Refer to the Biomek NX Multichannel Laboratory Automation Workstation User s Manual Chapter 2 Multichannel Pod for detailed information on the Multichannel Pod and interchangeable heads bee 1 tt Pein i Zz Multichannel Pod and he with extended gr A KZ Lf FANS ASS The deck is the work surface of the Biomek NX instrument and positions ALPs to be accessed by the Multichannel Pod CES 1 3 Multichannel Pod The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions Heads Interchangeable heads aspirate and dispense liquid using disposable tips The tip compatibility and maximum aspirate and dispense volumes vary for each head Power switch Light curtain Figure 1 1 Biomek NX with Multichannel Pod Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 1 4 Introducing the Biomek NX Laboratory Automati 1 2 1 Automated Labware Positioners ALPs Automated Labware Positioners ALPs are interchangeable structures that are installed on the Biomek NX deck in suitable positions to accommod
20. Clear current device setup of all labware after the method completes v Unload disposable tips from all pods after the method completes JV Clear all global variables after the method completes No Reporting 2 15 Check In Checking in a method is similar to saving a method but in addition creates a new revision of the method checks in all project items of the project file and creates new revisions for any items that have changed You will check in the method later in Section 3 4 Checking In a Method and Project File Quick Start Method BiomekNXMC BiomekNX MC ETC 0 00 42 Figure 2 14 Quick Start method saved and named Move on to Section 2 8 Running a Method to run your saved Quick Start method Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 16 Learning to Create Methods for the Biomek 2 8 Running a Method Next you will run the method you just created using the simulator The simulator allows you to watch the method on your screen without actually running the method on an instrument To run your saved method 1 If you do not see the Simulation Display button on the toolbar Figure 2 15 from the Instrument menu choose Hardware Setup Biomek Hardware Setup appears Biomek Hardware Setup p Reconnect ft Home All Axes Add Device s Remove Device Y Accept gt lt Cancel EE F Biomek NX SN None Serial
21. To retain the dimensions of predefined labware in Biomek Software it is important to copy and then edit the copy of the labware type rather than simply editing the predefined labware to meet the new specifications Copy Labware Type x Please enter a name Figure 3 3 Copy Labware Type 3 Enter NewLabwareQuickStart and choose OK The copy of BCFlat96 is created with the specified name and appears in Labware Type Editor Figure 3 4 Labware Types ne b x Filter lt any gt xl New Copy Delete Rename Edit Exit Greiner1536ConeF Greineri536FlatPE Greiner384ConePF Greiner384ConePP Greiner384FlatPP Greiner384 Lid Greiner384Therma Greiner36ConeHalf GreinerS6ConePP GreinerS6ConePS Greinerg6Flat Greiner6Roun 7 a rah FERA FE oon GreinerS6RoundPP GreinerS6RoundPS Greiner36Thermal Copy of BCFlat 96 renamed to NewLabwareQuickStart atse45qu Greinershallows34 IMReservoir96 sessseses SOSeoeeoese Figure 3 4 NewLabwareQuickStart in Labware Type Editor Beckman Coulter Inc Using the Editors While Creating Methods 3 7 4 Double click the NewLabwareQuickStart graphic NewLabwareQuickStart appears Figure 3 5 NewLabwareQuickStart Basic Information x Y Miscellaneous Span fi 2 7762 fe 5471 cm Movement Information Orbital Shaker Height fi 4224 em Ordering Information Stacking Colors Edit Wells_1 Bitmap FO es Description Beckman Coulter microplate with 96
22. When creating a new specifications are slightly different You must create a new labware type in the labware type it is Labware Type Editor to accommodate the difference Refer to the Biomek Software important that the User 5 Manual Chapter 7 Creating and Modifying Tip and Labware Types labware dimensions are TESRENASNEN E entered precisely per the Note Do not run a method outside of the simulator using the labware you create in americans this tutorial the specifications may not be accurate for the physical piece of labware you use in this method see sidebar specifications An incorrect labware definition could result in a number of problems 1 From the Project menu choose Labware Type Editor Labware Types including collisions appears Figure 3 2 gripper errors or poor pipetting performance To create new labware in the Labware Type Editor Labware Types ap Filter New Copy Delete Hename Edit Exit AB334WellReaction 4P384 30uL e eeeee tee LULIT TT TTT BCUpsideDownTil CostarCone36Rour Collar 36mm CollarSpacer_23mm Pm CostarDeep96Squat CostarFlat384Squz CostarFlat384Squ GranableRefilabler FilterHolder EE i Figure 3 2 Labware Types AP9I6_20uL_Barier Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 6 Using the Editors While Creating Methods 2 Click on BCFlat96 and choose Copy Copy Labware Type appears Figure 3 3 Note
23. ate Dest1 Note For information on the extensive selections available for configuring the Transfer step refer to the Biomek Software User s Manual Chapter 15 3 Configuring Transfer and Combine Steps 2 4 1 To insert a Transfer step into your method Inserting a Transfer Step Drag and drop a Transfer step from the Step Palette into the Method View just below the Instrument Setup step Notice that the Current Deck Display shows the current position of all labware on the deck Figure 2 7 You will complete three sections to configure the Transfer step for this method e Section 2 4 2 Configuring Tip Handling e Section 2 4 3 Configuring Source Labware e Section 2 4 4 Configuring Destination Labware Biomek Software Method1 New File Edit Project Instrument Execution Options Help PLACII CEIC xel Instrument Start Instrument Setup Use pod Pod1 x for transfer Tip Handling Setup JV Load AP96_200uL tips and unload them z when the transfer is done J Wash tips in p ater z p Ads of 7110 J Change tips between sources S oz i Finish IV Change tips between destinations Click here to add a source Stop when finished with Destinations x Dispense up to f1 time per draw Aspirate at most fo pL per transfer for repeated dispensmg Transfer Details Advanced Comment Current Deck Display
24. ate a wide variety of labware ALPs are either active or passive e Passive ALPs hold labware in place or act as receptacles for method by products or consumables Figure 1 2 e Active ALPS contain mechanisms that may use power and or air sources for various mechanical operations such as the Orbital Shaker and Stirring ALPs refer to the ALPs User s Manual Figure 1 2 Example of a passive ALP Note Refer to the ALPs User s Manual for detailed information on the ALPs on your system Beckman Coulter Inc nek NX Laboratory Automation Workstation 1 5 1 2 2 Light Curtain and Other Protective Barriers WARNING To reduce the risk of personal injury safety shields and light curtain must be in place before operating Safety shields and light curtain prevent entry into the work area during machine movement WARNING Dark non reflective material affects the sensitivity of the light curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex gloves do not degrade light curtain operation however it is advisable to test the impact of all lab attire on light curtain sensitivity before operating the Biomek NX gt amp The Biomek NX is equipped with protective barriers e Front light curtain e Side protective shields e Overhead canopy When an object larger than approximately 1 in diameter penetrates this protective zone the instrument shuts down immediately stopping all pod a
25. bware Properties Double click the labware on P5 and name it Dest1 in Labware Properties In Labware contains a select Known In volume enter 50 In liquid type select Water from the pull down menu Labware Properties for the BCFlat96 should look like Figure 2 5 Choose OK to close Labware Properties Labware Properties g Name Pest1 Labware Type BCFlat96 Maximum Volume 362 76 uL Bar Code Labware contains a known x volume 50 a uL of liquid type Water 7 Sense the liquid level the first time a well with Unknown or Nominal volume is accessed from the Liquid Sense the liquid level every time a well is accessed from the Liquid Show Labware Volumes OK Cancel Figure 2 5 Labware Properties for Dest1 Confirm that Pause to confirm setup is checked This will result in a confirmation prompt being displayed later when you run your method in Section 2 8 Running a Method Leave the Verify Pod Setup option as is This setup is used to inform the software of the current state of the pod for example if the pod already has tips loaded arning to Create Methods for the Biomek NX 2 7 Your Instrument Setup step is now configured and the main editor should look like Figure 2 6 Note To learn more about the Instrument Setup step refer to the Biomek Software User s Manual Chapter 15 2 Instrument Setup Step Biomek Software Method1 New i Fie Edit Project I
26. ca eens aces eendenensenneneeues 2 2 2 3 Configuring the Instrument Setup Step 00 0 2 4 24 Configuring a Transfer Step 424 scas4 nes as ba wet heursi esd duarsaarweas 2 8 2 4 1 Inserting a Transfer Step docs dee eeaitenaneeaidenaianked 2 8 2 4 2 Configuring Tip Banding y 0 00ve14 sau 64 ananuna 2 9 2 4 3 Configuring Source Labware 00 cece cece eens 2 10 2 4 4 Configuring Destination Labware 2 0 0 0 000 cece eee 2 11 2 5 Configuring a Move Labware Step 0 0 csc e cece ence ee esenees 2 13 2 6 Using the Finish Stopes lt scs lt die ded dee ad AS Abee oe dee eRe ee dd eee de de 2 14 2 6 1 Handling Configuration Errors 0 0 0 cece cece ee eee enee 2 14 2 7 Saving Method crcr cache a aeee nae egeednn UREE ER EDERE REEERE EA 2 15 2 8 R nnngaMethod srecrsceirrisrerer Rees edna Ekr G re EEE AEE Re 2 16 Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 Using the Editors While Creating Methods 3 1 OVETVIEW errad peder iara er ea eN Eea REA A Aer h 3 1 3 2 General Description of Editors 2 0 0 0 ccc cette nee 3 2 3 2 1 Project Mees srererg Bakers An aR ES eh E EES 3 3 3 2 2 Instrument Men peser eaweneeundawt eos eet ek eee beaten eed eae 3 3 3 3 Creating New Labware in the Labware Type Editor 3 5 3 4 Checking In a Method and Project File 2 0 0 2 eee 3 8 3 5 Creating a New Deck nnnunnnnn nnne con CaO
27. ditor showing steps available for insertion in a method They are located on the left of the main editor Use the Step Palette Builder to add other step palettes Refer to the Biomek Software User s Manual Chapter 29 5 Using the Step Palette Builder for more information jiomek NX Laboratory Automation Workstation 1 7 1 4 Starting the Biomek NX To start the Biomek NX you will follow the instructions in this chapter to turn on the Accounts amp instrument launch Biomek Software and home all axes Permissions When enabled Accounts 4 44 Turning On the Biomek NX amp Permissions is an integrated set of features To turn on the Biomek NX instrument built into the software to assist users in complying with electronic signature 2 Flip the switch to the On position The indicator light comes on in solid green requirements for closed systems The functionality for 1 4 2 Accounts amp Permissions is included in the Biomek Software installation and provides control over 2 If Accounts amp Permissions is enabled see sidebar a dialogue box appears who can create and run requesting you to log in using the user name and password assigned to you by methods and who may your system administrator Enter the required information Your account must only run validated have Develop Methods Develop Projects and Setup Instrument methods Refer to the permissions to complete all parts of this quick start
28. e left mouse button and move the hand up and down until the Auto Select is chosen in depth is as close to 1 00 mm from bottom as you can get a pipetting step such as Transfer each time a method is created Biomek Software OR automatically selects the technique most appropriate for the This will be your aspirate height for this transfer pipetting operation 4 Right click on the large tip illustration next to the microplate graphic in the configuration and choose Measure from the Bottom 6 To adjust the height precisely to 1 00 mm click on the tip and use the up or down arrow keys on your keyboard while holding the Alt key Right click on the graphic then select the height from the menu that appears The source labware configuration is complete and the main editor now looks like Figure 2 9 Yov ll now configure the destination labware to complete the transfer Biomek Software Method1 New File Edit Project Instrument Execution Options Help D s ORS Leo een nxe Start Use pod Pod1 gt for transfer ea Instrument Setup Y Load AP96_200uL tips change between sources and unload them when finished Setup Q Transfer Source Resv ig Finish E Reservoir x at Resv x Using liquid type water x IV Auto Select Customize 1 00 mm from botto Overrides Techni Stop when finished with Destinations x Advanced Dispense up to 1 time per draw C Aspirate at most jo uL per tra
29. eckman Coulter Inc policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Company warrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such one year period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows a Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list of such components and accessories is maintained at the factory b The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company will give reasonable assistance to Purchaser in obtaining from the manufacturer s own warranty c Any product claimed to be d
30. efective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportation charges collect unless the product is found to be defective in which case the product must be properly decontaminated of any chemical biological or radioactive hazardous material d The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company e Ifthe product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement Representatives and warranties made by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing
31. er Inc Learning to Create Methods for the Biomek NX 2 3 ZF As you can see the following two steps appear automatically when you drop the first step into the Method View e Start the first step in a method allows Variables Start creation of global variables that are applicable A variable is a value that to the entire method Refer to the Biomek Software Users Manual Chapter 12 2 1 Configuring the Start Step has been assigned a name that may be referenced repeatedly a e Finish the last step in a method provides during a method Refer Finish options to clear the Biomek NX deck clear the to the Biomek Software devices from the deck unload tips from the pod User s Manual Chapter and clear all global variables Refer to the 13 Using Variables and Biomek Software User s Manual Chapter Expressions in a 12 2 2 Configuring the Finish Step Method Proceed to Section 2 3 Configuring the Instrument Setup Step to configure the Instrument Setup step you just inserted Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 4 Learning to Create Methods for the Biomek N 2 3 Configuring the Instrument Setup Step CAUTION An inaccurate Instrument Setup may result in pod and A N labware collisions or in inappropriate pipetting When creating Biomek methods it is important to use the Instrument Setup step Obstacle along with the Deck Editor to
32. guide The Biomek Software Biomek Software User 5 main editor appears Figure 1 3 Manual Chapter 2 Using Accounts amp Note Ifyou are logging into Biomek Software for the first time you must enter Permissions a new password Remember your new password you will need to enter it each time you access Biomek Software 1 Locate the power switch on the right side of the instrument Figure 1 1 Launching the Software To launch Biomek Software 1 Choose Start gt All Programs gt Beckman Coulter gt Biomek Software Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 1 8 Introducing the Biomek NX Laboratory A Biomek Software Fie Edit Project Instrument Execution Options Help Ag ji pel Decaan ee K Instrument Setup Other editors are available from the Project and Instrument menus Step Configuration Area When a step is highlighted in the Transfer Combine Method View The method is built step by step in this area Comment I Basic Step Palette Displays the steps available for insertion into a method More steps are available through additional step palettes accessed from the Method View the corresponding configuration appears in this area Current Deck Display Changes to display the status of the deck upon completion of the previous step Options men
33. itors While Creating Methods Checking In a Method and Project File Checking in a method is similar to saving a method but also creates a revision record of the method checks in all project items of the project file and creates new revisions for any items that have changed You will check in your quick start method along with the new labware you just created To check in your method with the new labware 1 From the File menu choose Check In Check In appears with the new labware type Select the project items to check in Project Item Modified Time E M g Labware Classes Change La NewLabwareQuick E New mes Figure 3 6 Check In 2 3 2004 2 47 00 PM 2 Choose OK to check in the new revisions of project items Notice that the method name in the Biomek main editor title bar changes to Quick Start Method Revision 1 Development If Accounts amp Permissions is enabled other dialogues appear and more steps are required for checking in methods Enter a reason and your password to check in the method and project refer to the Biomek Software User s Manual Chapter 12 10 Checking In a Method Note Ifusing Accounts amp Permissions methods may also be validated by choosing Validate from the File menu see sidebar 3 5 Framing Framing teaches Biomek Software the location of ALPs and devices on the deck The software uses this framing information to move the pod and manipulate
34. l Control Pod1 Multichannel gt Vector Buld e gt Home D z Back Home Z xY Extend Gripper Left 4m vam gt Right Retract Gripper Move 2 Max D Units sie lt i gt cm Auchan Maes Squeeze Aspirate UnSgueeze Dispense CT pl M Current Position 36 895 cm Y 25 788 cm Z 22 cm D 0 2 cm Movement Vector x fo cm Y fo cm Z jo cm D jo cm M AutoClear Clear Speed 100 o Stop Figure 3 12 Advanced Manual Control for a Multichannel Pod Select Move Z Max The pod moves to the highest configured height Select Close Advanced Manual Control Pod1 closes From Manual Control click on P5 The pod moves to position P5 on the deck NH Wn A U You can click on additional positions to move the pod to that position When you are finished moving the pod with Manual Control choose Exit to close Manual Control 7 From the toolbar chose Close to close the quick start method OR From the File menu choose Close The method closes and you are now ready to create a method of your own Beckman Coulter Inc Using the Editors While Creating Methods 3 13 3 7 Quick Start Guide Summary Congratulations You ve just completed this quick start guide You ve completed e a small method e steps using the editors e steps creating some new project items e steps to manually control the Biomek NX If you ve used the links to other manuals you ve a
35. lso learned where to find hardware information on the Biomek NX ALPs and more information on Biomek Software Enjoy your new Biomek NX Multichannel Laboratory Automation Workstation Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide
36. mp Instrument Setup Load AP96_200uL tips change between sources and unload them when finished Setup a Transfer Click here to add a source lg Finish Use this arrow to collapse and expand Tip Handling view Stop when finished with Destinations x Dispense up to 1 time per draw Aspirate at most fo uL per transfer for repeated dispensing Transfer Details Comment Method1 BiomekWXMC BiomekNx MC ETC 0 00 03 Figure 2 8 Transfer step with Tip Handling collapsed Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide Learning to Create Methods for the Biomek NX 2 4 3 Configuring Source Labware To configure the labware named Resv as source labware in the Transfer step 1 Click on Click here to add a source 2 Click on Resv labware sitting on P4 in the Current Deck Display Note The information you supplied during Instrument Setup is displayed in the source labware configuration 3 Leave the Auto Select checkbox selected to use the technique chosen by Techniques Biomek Soft iomek Software Techniques allow pipetting settings to be saved globally and used in any method Biomek 5 To set the aspirate height for the depth the tip descends into the reservoir place Software includes several the mouse cursor over the tip illustration When the cursor turns into a predefined techniques If hand hold down th
37. nd head operations Some ALP operations such as shaking or stirring continue refer to the ALPs User 5 Manual When the instrument is sitting idle or in the paused mode no violations are registered when the protective zone is penetrated Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 1 6 Introducing the Biomek NX Laboratory Automati 1 3 Biomek Software Biomek Software is designed to Method e Do a substantial amount of the method building work for you Allow you to take as much direct and precise control over the method building process as you want A method is a series of steps controlling the operations of your Biomek NX The main editor that appears when the software is launched Figure 1 3 is used to build and configure methods Beckman Coulter Inc e Each step is dragged and dropped from a Step Palette into the Method View refer to Chapter 2 Learning to Create Methods for the Biomek NX When a step in the Method View is highlighted the corresponding configuration options appear in the Step Configuration Area refer to Chapter 2 Learning to Create Methods for the Biomek NX The method is customized to the desired level of precise control via the editors accessed from the Project and Instrument menus refer to Chapter 3 Using the Editors While Creating Methods Step Palettes Step Palettes are panes in the Biomek main e
38. nsfer for repeated dispensing Y Transfer Details Method1 BiomekXMC BiomekNx MC ETC 0 00 03 Figure 2 9 Transfer step with source labware configured Beckman Coulter Inc Transfer Details Transfer details include a stop condition for transfer operations a repeat pipetting configuration and a maximum tolerance in timing between transfer operations Refer to the Biomek Software User s Manual Chapter 15 3 4 Configuring Transfer Details 2 4 4 Learning to Create Methods for the Biomek NX 2 11 Configuring Destination Labware To configure the labware named Dest as destination labware in the Transfer step 1 Before you configure the destination labware click the down arrow in Transfer Details located just above the Current Deck Display to collapse that section You will not be making any changes to the options in this section Click on Dest sitting on P5 in the Current Deck Display This one operation accomplishes the same task as steps 1 and 2 of Section 1 5 2 Configuring Source Labware Note Notice that the source labware configuration is collapsed and replaced with a text summary of the setup If you want to reopen the source configuration click anywhere in the collapsed area The Volume field is highlighted which allows you to designate the amount of liquid to be dispensed For this tutorial you Il transfer 100 uL so type 100 into the
39. nstrument Execution Options Help PEIC CCORI CEIC amp Start Deck Joeckt 7 JV Pause to confirm setup Pause for bar code input ie Instrument Setup Labware Category lt Any gt IV Verify Pod Setup Configure B Finish BCSPECol Collar 36r CollarSpa CostarCe CostarDe Costal CostarFl Drainable FilterHolc Greinerl Greiner1t Method1 BiomekNx MC BiomekNX MC ETC 0 00 02 Figure 2 6 Instrument Setup step with labware named Proceed to the next section to configure a Transfer step in your method Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 8 2 4 Transfer Step A Transfer step loads tips aspirates and dispenses liquid and unloads tips This eliminates the need to insert four separate steps to complete a liquid transfer However there are also steps in Biomek Software that allow these steps to be performed individually using the Intermediate Step Palette refer to the Biomek Software User s Manual Chapter 16 Using the Intermediate Step Palette Learning to Create Methods for the Biomek N Configuring a Transfer Step The Transfer step transfers liquid from a single source to one or more destinations You are going to insert a Transfer step into the method and configure it to move liquid from your source reservoir ReSv to your destination micropl
40. oximately 0 41 Note Your ETC may vary slightly That s OK Refer to the Biomek Software User s Manual Chapter 12 2 2 Configuring the Finish Step 2 6 1 Handling Configuration Errors If there is a step configuration error in your method the step with the error is highlighted in red Figure 2 12 and the ETC is displayed as Failed when you click on the Finish or any step that occurs after the one with the error The error message also is displayed in the status bar or when hovering the mouse over the highlighted step Use the displayed message to correct the error and reconfigure the step Note Refer to the Biomek Software User s Manual Chapter 25 Handling and Preventing Errors Biomek Software Method1 New File Edit Project Instrument Execution Options Help ALCELI CELERE Instrument Setup Transfer Combine Comment Start Instrument Setup Clear current device setup of all labware A UCAC oN after the method completes N Move Labware from P Vv Unload disposable tips from all pods i Vv Clear current instrument setup of all labware after the method completes after the method completes IV Clear all global variables after the method completes Error Message The error message is displayed in the status bar and as a tool tip ETC Displayed as Failed when there is an error feporting Error trying to dispense to PS coming from P4
41. rs for customizing a method To understand the depth of operating opportunities designed into this system as well as to familiarize yourself with the suite of comprehensive user s manuals made available at installation links are provided throughout this guide to specific sections in the Biomek NX Multichannel Laboratory Automation Workstation User s Manual the Biomek Software User s Manual and the ALPs User s Manual In addition to the instructions graphics and links to other sources you will see in this guide additional supportive information in the following forms Definition Important Concept These boxes contain r brief definitions of terms These boxes emphasize and concepts that may important concepts be specific to the relevant to the Biomek Biomek NX or NX instrument and generally unfamiliar Biomek Software Refer to the links in this quick start guide or the Biomek NX Multichannel Laboratory Automation Workstation User s Manual or the Biomek Software User 5 Manual for more information on these important concepts 1 2 iomek NX Laboratory Automation Workstation Biomek NX The Biomek NX Figure 1 1 is a multiaxis instrument designed with an open architecture to allow expandability of the system The design provides a foundation for interchangeable specific use components such as the 96 and 384 Channel Heads and a variety of ALPs refer to Section 1
42. rument Setup step from the palette into the Method View A new method is created and assigned the default name Method1 New Figure 2 1 Note The Instrument Setup Step tells Biomek Software where you have physically placed labware on your deck Biomek Software Method1 New l0 x Fie Edit Project Instrument Execution Options Help PECI ELI DEIC o amp Start Deck Deckt x aa Instrument Setup Labware Category lt Any gt x Setup Step Configuration Area verify p When a step is highlighted in the 4 Method View the corresponding configuration appears in this area IV Pause td Q ig Finish E E mee Method View The method is built step by step in this area BCUpsid CostarCe CostarFl Costal Drai il Greineri Greiner 5 Collar 36r CollarSpa CostarDe Drainable FilterHolc i Brainard GuninavIe rainards Greiner rainart Grainarat GrainarGt Arainarak Basic Step Palette Displays the steps available for insertion into a method Additional steps are available through other step palettes Current Deck Display Displays the status of the P8 deck upon completion of the previous step Comment Methodi BiomekXMC BiomekNX MC ETC 0 00 02 Figure 2 1 Biomek Software main editor for multichannel Biomek NX Beckman Coult
43. s chapter and complete the tutorial steps provided After you have completed the instructions or if you need additional information along the way use the links provided to access the Biomek Software User s Manual In this chapter you ll learn General Description of Editors Section 3 2 e Creating New Labware in the Labware Type Editor Section 3 3 e Checking In a Method and Project File Section 3 4 e Creating a New Deck Section 3 5 Manually Controlling the Pod Section 3 6 Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 3 2 Using the Editors While Creating Methods 3 2 General Description of Editors If using Accounts amp Permissions You must have Develop Projects and Setup Instrument permissions to access the editors on the Project and Instrument menus Refer to the Biomek Software User 5 Manual Chapter 2 Using Accounts amp Permissions for more information Biomek Software Several editors are used to configure Biomek Software to control your Biomek NX instrument Editors are accessed from one of two menus e Project menu Figure 3 1 OR Instrument menu Figure 3 1 File Edit Instrument lt xecutien Options Help De cf Go Ee P xo c AlS m E xel Instrun Sety Labware Tran TIP Type Techniqu Liquid Ty Pipetting Comb Includes access to Includes acce
44. ss to Project Instrument Type Editor Hardware Setup Editor Deck Editor e Browser Device Editor pe Editor Manual Control Template Editor Import Export Utility SY k Comment BiomekNX MC BiomekNX MC ETC Beckman Coulter Inc Figure 3 1 Biomek main editor Following is a brief description of the editors available from the two menus displayed in Figure 3 1 If you would like to learn more about a specific editor refer to the referenced chapter for more information You will work with two of the more commonly used editors and Manual Control in this quick start guide Using the Editors While Creating Methods 3 3 3 2 1 Project Menu The editors on the Project menu Figure 3 1 allow project items to be created copied revised and renamed They include the following e Labware Type Editor allows you to add new labware types copy labware types delete labware types rename labware types and edit labware type properties refer to the Biomek Software User s Manual Chapter 7 Creating and Modifying Tip and Labware Types Note Refer to Section 3 3 Creating New Labware in the Labware Type Editor in this quick start guide to follow step by step instructions to create a new piece of labware e Tip Type Editor allows you to add new tip types remove tip types and change tip properties refer to the Biomek Software User s Manual Chapter 7 Creating and Modifving
45. stem and related documentation or the procedures specified in this manual regardless of foreseeability or the form of action whether in contract tort including negligence breach of warranty strict liability or otherwise and including but not limited to damages resulting from loss of data loss of anticipated profits or any special indirect incidental or consequential damages In no event shall Beckman Coulter Inc s liability to the user exceed the amount paid by the user to Beckman Coulter Inc hereunder The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results Beckman Coulter SAGIAN Operations Indianapolis Indiana www beckmancoulter com Biomek Beckman Coulter and the Beckman Coulter logo are registered trademarks of Beckman Coulter Inc Microsoft and MS DOS are registered trademarks and Windows is a trademark of Microsoft Corporation All other trademarks appearing in this manual are owned by their respective companies Copyright 2004 by Beckman Coulter Inc All rights reserved No part of this publication may be reproduced distributed or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise or stored in a database or retrieval system without the prior written permission of Beckman Coulter Inc Beckman Coulter Inc Warranty and Returned Goods Requirements All standard B
46. t a Move Labware step into the Method View Manual Chapter 2 3 after the Transfer step Gripper 2 In Move labware from select P5 from the drop down menu 3 In Move labware to select P8 from the drop down menu The main editor should now look like Figure 2 11 Biomek Software Method1 New Fie Edit Project Instrument Execution Options Help Dee ORS Y DELCE i Start Using pod Podt 7 UN rs Instrument Setup Move labware from P5 x to Setup Q b Transfer 100 uL from Move the entire stack of labware 1 Move Labware from P C Move stack leaving the bottom piece of labware at the source position Transfer rar es Ket C Move the topmost 1 piece of labware from the stack Finish Comment E Laced Method1 BiomekXMC BiomekNX MC ETC 0 00 32 Figure 2 11 Configured Move Labware step The Move Labware step configuration is complete Now move on to Section 2 6 Using the Finish Step to learn how long the method will take to run Biomek NX Multichannel Laboratory Automation Workstation Quick Start Guide 2 14 Learning to Create Methods for the Biomek 2 6 Using the Finish Step Use the Finish step to see how long it will take to run your method 1 Click on the Finish step in the Method View 2 Check the status bar at the bottom of the main editor for a display of the Estimated Time for Completion ETC For this method the ETC is appr
47. tep uses a deck layout display as a map for the placement of labware ALPs and external hardware devices such as stacker carousels CAUTION Do not place labware other than a tip box on a tip loader 2 Drag and drop the following labware from the available labware list onto the Creating Labware Deck Layout Display HOU wantin use an AP96_200uL to TL1 labware type that does not appear in the e a Reservoir to P4 available labware list use lt a BCFlat96 to P5 the Labware Type Editor to create a new Note To remove unwanted labware from the Deck Layout Display during labware type Refer to setup drag and drop the labware to the Clear trash icon Section 3 3 Creating New Labware in the Your Biomek NX main editor should look like Figure 2 3 Labware Type Editor Biomek Software Method1 New 7 2 File Edit Project Instrument Execution Options Help Available labware list D amp a Rs Belo a 01 O Drag the desired labware graphic to the Start Deck Decki x N desired position on the Deck Layout Display RA E Instrument Setup Labware Category lt Any gt 7 Setup IB Finish Hm e i Collar 36r CollarSpa CostarCe CostarDe CostarFl CostarFl Drainable FilterHole Greinerl Greinerit Reservoir L Deck Layout on P4 Display Comment From the Deck Layout Display double click the labware graphic to display Labware Properties
48. u 1 4 3 MC ETC Figure 1 3 Biomek Software main editor Homing All Axes of the Biomek NX Pod Every time the Biomek NX is turned on yov ll have to Home All Axes This automatic process initiates the pod and defines the home position from which all subsequent moves are determined To home all axes 1 From the Instrument menu choose Home All Axes A Warning appears 2 Make sure that all warnings are addressed and choose OK The pod is homed and the instrument is now ready for use Beckman Coulter Inc Learning to Create Methods for the Biomek NX 2 1 Overview In this chapter you will be given information and instructions for building a small method using Biomek Software A method is a series of steps that control the operation of your Biomek NX The method you complete in this chapter represents basic operations and is intended simply to familiarize you with the Biomek NX and to create a basic understanding of method building opportunities Read the information in this chapter and complete the tutorial steps provided After you have completed the instructions or if you need additional information along the way use the links provided to access the suite of user s manuals provided during installation These manuals include Biomek Software User s Manual Biomek NX Multichannel Laboratory Automation Workstation User 5 Manual and ALPs User s Manual Each manual contains comprehensive information
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