Warner Instrument Corporation
Contents
1. priming gently shake the submerged pump in the reservoir to remove any trapped air from the pump body Proper filling of the pump with water will result in its quiet operation Once fluid is flowing and all lines are filled check for leaks and top off the reservoir to the bottom of the handle before sealing the reservoir cap The system is now ready for use J Warner Instruments BLM TC Manual Rev 060331 9 OPERATION CL 100 bipolar temperature controller Temperature control of the BLM TC is maintained by the CL 100 Please review the user s manual for this instrument for its operation All aspects of the CL 100 function are compatible with the BLM TC This includes use of the T2 MONITOR THERMISITOR CAUTION While the CL 100 provides control over the Peltier device within the platform it is the cooling action of the water jacket which prevents failure or even destruction of the Peltier device during use Therefore it is absolutely imperative that the pump be running AT ALL TIMES while power is applied to the PELTIER PLATFORM This condition remains true regardless if the device is being used for heating or for cooling BCH M13 BCH M22 bilayer chamber While much attention in this manual has focused on the Peltier component and it s cooling system the heart of the device resides within the bilayer chamber Warner offers thermocycler bilayer chambers in two sizes 1 ml BCH M13 and 3 ml BCH M22 These chambers ha2. BLM TC Manual Rev 060331 Marner Instruments Planar Lipid Bilayer Thermocycler Model BLM TC Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax J Warner Instruments BLM TC Manual Rev 060331 Table of Contents INTRODUCTION roi 5 NOMENCLATURE c AAA EAE A E EA E 4 Text CONY CIUIONS ss cssssssccccesens cesavnesssaseicnctcssascctetsapasesaveessaeshicns sevesndecisusaddacsescuadunzeiasbiescsenmuwsssesnenictecsiereicts 4 INTRODUC Neca scecctesdnnn sa ccts vacdecenunsstscauccissisussscstaccdecesunsstssauceisscsusssastsventecesuvsstesauccissisuxssastevactesesuesaes 5 SETUP oo pene erie err BP rr ee E ene enero rere eer eter eer reer reer errr reece rere 6 General COC ECU C scarccsiecevseaceceeensasosecncuaeyssnseshnsvatesusvssucesasecesevosncesssccuseds yee eocnseeadeassetysecnssiaseevsenzeseesuseuies 6 Step 1 CL 100 placement cssccccecccccccccccocccccccccecceccococeccocssoscseccccececececcocoososoccceccecececeesccossssssssocccesceceeee 6 Step 2 Water pump assembly placement ccccccccssssssssssscccccssssssssssccccccsccssssscssccccsscsssssssssccesssseass 6 SED Pelier Platform PlACEMEIE sscccscsscsicessssescessiaccsasescocs cesses tevscecsacesescussescossasevscceseoesessievsexcseoeeceenes 7 Step 4 Warer jacket decoupler placement xiscssccecseisssisssceecscestasesssecosasevesasncassussadevesbescauscvssesetecedsenssse s 7 Step S COMnectime the water Ines ecace
3. M TC Manual Rev 060331 4 NOMENCLATURE Text conventions This manual refers to controls at two functional levels specific controls and settings of these controls To minimize the potential for confusion we have employed several text conventions which are specified below Since our goal is to provide clarity rather than complexity we welcome any feedback you may wish to provide Warner Instrument product numbers are presented using bold type gt References to components are specified using SMALL CAPS gt References to control settings are specified using ale type gt Special comments and warnings are presented in highlighted text Any other formatting should be apparent from context i Warner Instruments BLM TC Manual Rev 060331 5 INTRODUCTION The BLM TC is a Peltier driven thermocycler specifically designed for use with Warner s Planar Lipid Bilayer Workstation A complete thermocycler system is comprised of 4 major components a special BILAYER CHAMBER the PELTIER PLATFORM a BIPOLAR TEMPERATURE CONTROLLER and a pump drtiven PLATFORM COOLING SYSTEM Actual temperature control both heating and cooling is provided by a thermoelectric device embedded within the PELTIER PLATFORM Heat energy is added or removed from the solution surrounding the bilayer membrane by a thermally conductive material inserted into the body of a special bilayer chamber the BCH 13M or BCH M22 chambers These chambers in turn are
4. ck or other convenient location The PELTIER PLATFORM within the Faraday cage will eventually connect to the CL 100 via its connecting cable Make all necessary ground and power connections at this time see CL 100 User s Manual but do not power the instrument Step 2 Water pump assembly placement Next position the WATER PUMP ASSEMBLY at a convenient location near the CL 100 and Faraday cage While one option is on the floor below the bilayer setup bear in mind that the lift capability of the pump is J Warner Instruments BLM TC Manual Rev 060331 7 limited to 1 m 3 feet The WATER PUMP ASSEMBLY will eventually connect to the WATER JACKET DECOUPLER on the back of the Faraday cage via the supplied Tygon tubing Plug the water pump assembly into the supplied power strip but do not power the device at this time Step 3 Peltier platform placement Place the PELTIER PLATFORM within your Faraday cage and run the controller cable out through the Faraday cage access port to the rear of the CL 100 Make a connection between the PELTIER PLATFORM and the CL 100 The PELTIER PLATFORM is supplied with a ground cable attached to it Make a connection from the PELTIER PLATFORM to the common ground within your Faraday cage Step 4 Warer jacket decoupler placement The WATER JACKET DECOUPLER attaches to the side wall of your Faraday cage at the access port and provides grounding of the water lines entering the ca
5. ge The DECOUPLER attaches to the cage wall via a set screw which pierces the cage material providing a ground connection at that point Place the WATER JACKET DECOUPLER onto one side of the access port on the rear of the cage and tighten it into place using the supplied Allen wrench Step 5 Connecting the water lines Water lines run in a circular path from the PUMP ASSEMBLY to the DECOUPLER to the PELTIER PLATFORM and back again Begin by running a short length of Tygon tubing from each attachment port on the rear of the PELTIER PLATFORM to the inside attachment ports on the WATER JACKET DECOUPLER J Warner Instruments BLM TC Manual Rev 060331 8 Next run a pair of water lines from the external attachment ports on the DECOUPLER to the top of the pump assembly Make sure all connections are secure avoid leaks Step 6 Loading the pump reservoir and checking flow Verify that the PUMP ASSEMBLY is unplugged Open the reservoir by removing the cap on the reservoir top Fill the reservoir with clean water up to the bottom of the handle Verify that all flow lines between the PUMP ASSEMBLY and PELTIER PLATFORM are secure and plug the pump into the supplied power strip Turn the power strip on to activate the pumping action It is likely that on first use the pump will need to be primed This need will be apparent by 1 a lack of fluid flow in the lines and 2 a lot of noise coming from the pump If the pump needs
6. heated or cooled via a tight physical coupling between the chamber and the PELTIER PLATFORM While the temperature of the PELTIER PLATFORM is set by the BIPOLAR TEMPERATURE CONTROLLER Warner model CL 100 excess heat is removed from the platform by a pump driven circulating water bath This cooling system is provided only as a means to keep the associated Peltier device within its thermal operating limits A great deal of effort has been dedicated towards making the pump drive both mechanically and electrically quiet i Warner Instruments BLM TC Manual Rev 060331 6 SETUP Begin by taking assessment of the supplied components These include the PELTIER PLATFORM a WATER PUMP ASSEMBLY a WATER JACKET DECOUPLER a CL 100 BIPOLAR TEMPERATURE CONTROLLER 25 feet of Tygon tubing and a power strip Additional components you should have on hand are the BCH M13 or BCH M22 chamber along with its CUP CAP and a bilayer cup These items are shown below Peltier platform water jacket decoupler ml j Sms cup cap BCH M13 General procedure The general setup procedure is to install the CL 100 into your equipment rack place the WATER PUMP ASSEMBLY near the setup position the PELTIER PLATFORM in the cage connect the WATER JACKET DECOUPLER to the cage wall and to make necessary connections between components Step 1 CL 100 placement The first step is to install the CL 100 into your equipment ra
7. ight check the fuse at the rear panel located in the black POWER INPUT MODULE If the fuse is found to be defective replace it with a 5x20 mm 0 5 A slow blow fuse 0 25 A for facilities using 220 240 V line voltages If the replacement fuse also fails please call Warner Instruments for assistance B Occasionally a knob on the front panel will loosen after long use These are collet style knobs and are tightened with a screw located under the knob cap To gain access to the adjustment screw pry the cap off with a thin bladed screwdriver or similar tool C Should service be required please contact the factory The problem may often be corrected by our shipping a replacement part Factory service if required will be expedited to minimize the customer inconvenience D Instruments are inspected immediately upon receipt and the customer is notified if the repair is not coveted by the warranty Repairs can often be completed in 1 2 days from our receipt of the instrument E If factory service is required please observe the following instructions 1 Package the instrument with at least 3 inches of cushioning on all sides Use the original shipping carton if it is available 2 Insure the shipment for its full value 3 Include with the shipment an explanation of the problem experienced i Warner Instruments
8. ned as a thermocycler we have provided CUP CAPS which can be placed on the top of the bilayer cup before the beginning of the experiment A small slot with PE tubing is provided in the CUP CAP to allow access of the silver wire electrode i Warner Instruments BLM TC Manual Rev 060331 10 Warranty and service Warranty The model BLM TC is covered by our Warranty to be free from defects in materials and workmanship for a period of one year from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not cover instrument failure or damage caused by physical abuse electrical stress inputs exceeding specified limits or pump failure In the event that instrument repairs are necessary shipping charges to the factory are the customer s responsibility Return charges will be paid by Warner Instruments Inc Normal business hours are 8 30 AM to 5 30 PM EST Monday through Thursday and 8 30 AM to 5 00 PM on Friday Our offices are located at 1125 Dixwell Avenue Hamden CT 06514 and we can be reached by phone at 800 599 4203 or 203 776 0664 Our fax number is 203 776 1278 In addition we can be reached by e mail at support warneronline com or through our Web page at http www warneronline com CL 100 Service notes Please refer all questions regarding service to our Engineering Department A If the instrument POWER light fails to l
9. scesseshessccerecsssevessctecncecessaveaccsneszecoussusces ccbascesseveeseccensecentaveeseceaniess 7 Step 6 Loading the pump reservoir and checking flow ssssssssecccccsssscecccocsssssecococsssssececoosssssecesesssse 8 RO TIN areca E E E E EE EE ES 9 CL 100 bipolar temperature controller ccsocssssseccccccsssecececcossssessececsossssecececsssssscccocssssssseeecsssssssssesoo 9 BCH M15 BCH M22 bilayer chamber scssesssscscavesccetseressescaccccatansescassoscesccasasestanvssesccusescarenssacscauessisss 9 IOE Ce 1 PEPE A S TE E E E EON SE EE IE ET OEA 9 Warr y AIC SOTVA E 10 E oee E osseeaceatedetctessdesaacdee wea emneetosanseciueedenasetesesacen 10 CEUS I T O T T T T EA ee 10 i Warner Instruments BLM TC Manual Rev 060331 3 The BLM TC Planar Lipid Bilayer Thermocycler from Warner Instruments is a versatile and simple to use thermal control device This unique device uses Peltier technology to provide both heating and cooling to a planar lipid bilayer membrane Total automatic control of heating cooling power is provided by a Warner CL 100 Bipolar Temperature Controller Features of the BLM TC include V Optimized specifically for use on planar lipid bilayer studies Peltier driven technology Heats and cools Quiet operation does not interfere with single channel recording Automatic and manual modes XK NNN Simultaneous monitoring of system temperature and separate point of interest i Warner Instruments BL
10. ve a thermally conducting assembly attached to their body which serve to transfer heat from the PELTIER PLATFORM to the solution surrounding the bilayer membrane Begin by preparing and loading your bilayer cup in the usual manner Place the cup into the BCH xxM chamber and prepare the chamber for use as you would normally Place the cup chamber assembly into the PELTIER PLATFORM and make any necessary attachments to the headstage You may also place and adjust any stirbars you will use at this time Once a stable membrane has formed temperature control may be applied via the CL 100 As always verify that the pump is supplying water to the PELTIER PLATFORM before supplying power to the PELTIER PLATFORM The rate of thermal transfer to the bilayer cup and chamber is on the order of 3 5 C min The actual temperature within the either the cup or chamber can be directly measured using the supplied T2 MONITOR THERMISTOR Cup cap A performance difficulty is revealed whenever the BLM TC is used for cooling of a bilayer membrane In a word condensation In general condensation occurs whenever the temperature of an object goes below the ambient dew point In a bilayer environment the effect of condensation is to provide an undesirable conducting pathway connecting the ws and rans sides of the bilayer membrane This is most often revealed as a highly variable large scale offset current which is sudden in onset Since the BLM TC is desig
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