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UviLine 9100-9400

1. Press F4 key Open to enter in the safeguard sub menu Press F1 key Location Select USB memory Give aname Confirm with lt START E NTER gt key If the safeguard in csv format were not carried out the measurement data are lost when the user leaves the measurement mode Remark With kinetic recordings the current measurement is always saved in the KineticsBackup csv file for safety reasons 11 3 6 1 2 Spectrum and kinetic curve are memorized inside the Internal file DataB already lt HOME gt Spectre Cin tique Open Downloading of spectrum or kinetics curve lt STORE gt Location Select USB memory Memory 209 Press F4 key Open Select a spectrum or kinetics curve Confirm with lt START E NTER gt key Press lt STORE gt key to open save dialog box Press F1 key Location Select USB memory Change the name if necessary Confirm with lt START ENTER3 gt key 11 3 6 1 3 Absorption Transmission Concentration Multi wavelengths mode lt HOME gt Concentration Absorbance Transmission Multi wavelengths Setup or General setup from the main menu Measured value memory Selection of files to be exported lt STORE gt Location Select USB memory The measurement data of Absorbance Transmission Concentration and multi wavelengths are stored in the measurement data memory first either automatically
2. Select Edit method with A and Y keys then confirm with lt START ENTER gt key 154 Absorption Transmission mode 7 Absorption Transmission Mode 7 1 General The absorbance or transmittance is measured respectively without the use of any methods All settings are configured during measurement The absorbance or transmittance can alternatively be measured against the air or against a reference so lution determined by the operator lt HOME gt Absorption transmission If necessary come back to the main menu with lt HOMEs gt key Select with A and W keys Absorption transmission mode Home w 03 25 08 14 03 Absorbance Transmission Multi wavelengths Spectrum Kinetics Enter in the absorption mode It displays Absorbance 03 25 08 14 04 Zero measurement required Setup The settings of the last measurement are active Absorption Transmission mode 155 7 2 Measurement Press F2 key Wavelength to enter a new wavelength Press lt ZERO BLANKS key to start the zero measurement Press lt START ENTER gt key to start the sample measurement It displays 0 537 29 0 Start measurement with lt START ENTER gt Continuous Absorbance and transmittance are displayed Press lt START ENTERS key to start a new measurement 03 25 08 14 14 0 340 45 7 0 589 29 8 Yo 0 345 45 2 Yo 0 467 34 1 o 0 537 29 0 Start me
3. Select method all 03 25 08 16 30 Remark Note the case sensitivity when searching 164 Multi wavelengths mode 8 7 Method application lt HOME gt Multi wavelengths Select method Select method all 03 25 08 16 35 Be 2001 PROT majmi 2002 DNA DNA Purity Select a method with A and W keys and confirm with lt START ENTERs gt key It displays Multi wavelengths 03 25 08 16 42 Zero measurement required 2002 DNA Method list Press lt ZERO BLANK key Zero is performed at the programmed wavelengths Then it displays Multi wavelengths H 03 26 08 13 34 Start measurement with lt START ENTER gt 2002 DNA Press lt START ENTER key to start the sample measurement Multi wavelengths mode 165 woven BIE ove os A 280 nm 2 220 A 260 nm 0 925 2 138 mom 2002 DNA DNA Furity Method list The spectrophotometer runs the measurement automatically Press on F3 key Transmission to have the transmission values It displays at the same time the absorbance values or transmittance values of the sample read at the differ ent wavelengths Press on lt START ENTER gt key to run a new measurement 166 Multi wavelengths mode 8 8 Printing results UviLine 9100 7449001 1 14 Administrator Time of measurement 07 01 2008 14 05 46 Method 2002 Measured value 1 02 1 000 A 260 nm Function R 1 000 A 280 nm 35 57 R
4. Setup Deactivate user management Change password Select Deactivate user management and confirm It displays User management 213 Name User group Adm Deactivate user management Deactivating deletes all entries in the user management If the user management is reactivated later it has to be readjusted Change Select OK and confirm The user management function is inactive Each user has administrator rights REMARK If the user management is deactivated by a user of the Administrator user group all user accounts that were set up are lost The password for the administrator is reset to admin 12 4 Creating editing and deleting a user account Bei aktivierter Benutzerverwaltung kann ein Benutzer mit Administratorrechten Benutzerkonten verwalten 12 4 1 Creating a user account lt HOME gt General setup User management Ada During the creation of a user account the Name User group and Password are defined Name User grou Admir ER Enter user name Change 1 Press F2 key Add The input field for the new user name opens up 2 Enter the user name lt A 9 gt and confirm The selection field for the user group Administrator User opens up 3 Select and confirm the user group The input field for the password opens up 4 Enter the password lt A 9 gt and confirm The user account is created and appears in the list of user accounts 214 Us
5. Standard printer ink or laser connected to the USB A port 202 Memory fat The printer icon indicates that the display contents can be printed To print press lt PRINT gt key The complete displayed list is then printed The filter settings are applied to this printing operation 11 3 2 3 Transferring data to PC Hyper terminal The data can also be received by a PC with a terminal program instead of a printer For this the PC is con nected to the photometer via the RS2382 interface The output is identical 11 3 3 Recording results 11 3 3 1 Manual recording After each measurement it is possible to store the measurement data manually with the lt STORE gt key It is stored in the measurement data memory The memory symbol E in the header indicates that the measure ment data displayed on the screen is ready to be stored With the measuring modes Concentration and Multi wavelengths there is the additional option to automatically store all new measured values at the time of the measurement AutoStore see paragraph 11 3 3 2 When storing manually an input field for the identification ID appears after pressing the lt STORE gt key Here it is possible to enter an individual combination of alphanumeric characters for later easier identification of the measurement datasets 30 digits are available for this WARNING MODE Absorption transmission In Absorption transmission mode the AutoStore function
6. function it is possible to hide all measurement datasets that corre spond to the specified criteria of the filter see paragraph 11 3 4 Remark It is possible to use this function to select and delete measurement datasets no longer used Measurement data me EZ ee 16 00 03 12 08 10 gt 50 00 ma NO3 Administr 03 12 08 10 57 gt 50 00 mg l NOS Administr cee 03 12 08 10 56 03 12 08 10 56 03 12 08 10 55 03 12 08 10 55 03 12 08 10 55 03 12 08 9 50 20 40 ma NO3 Administ Assay01 20 40 mg l NOS 12 60 mg l NO3 12 60 mg l NOS 12 60 mg l NO3 9 00 mg l NO3 Administr Administr Autostore Test0 Test01 AutoStore AutoStore 03 12 08 9 49 09 00 mol MO Administr AutoStore 03 12 08 9 49 9 00 mgANO3 Administr AutoStore 03 12 08 9 49 9 00mg NO3 Administr AutoStore Filter v SE allocation Se 1000 The list of the results which are not taken into account in the criteria of selection is displayed These results are isolated from the results taken into account by the criteria of selection 11 3 4 3 Filtering a single result lt HOME gt Concentration Absorption transmission or Multi longueurs d onde Setup or General setup from main menu Measured value memory Single value Measurement data me 03 18 08 16 16 03 18 08 16 14 16 98 mgl NO3 w AutoStore 03 18 08 16 13 104 55mg NO3 w AutoStore 03 18 08 16 13 70 89 mgA NOS AutoStore 03 18 08 15 22 1 00 A1 A2 ASSA
7. See paragraph 11 3 3 2 or manually with the lt STORE gt key see paragraph 11 3 3 1 Data stored in the measurement data memory can be filtered with filter criteria and then exported in the PC readable in csv format Press F1 key Setup inside one of the measuring mode or General setup from the main menu If necessary set any filter criteria with Setup Press lt STORE gt key to open a save dialog box The spectrophotometer automatically suggests the location Internal DataB folder and a file name If necessary use F1 key Location to change the location USB memory If necessary change the suggested file name Press lt START ENTER gt to save the selected results Data are saved 11 3 6 2 Copying all files onto a flash drive If no PC is directly connected to the photometer you can very easily transmit all files containing measurement data from the spectrophotometer Internal DataB folder to a connected USB memory lt HOME gt General setup Save all data on USB memory General setup 03 18 08 17 58 Language Date Time Display settings User management Measured value memory Software update Reset Data transfer Printer Save all data on USB memory 210 Memory Set the USB memory in place Press F1 General setup key Select Save all data on USB memory Wait few seconds It will display Save data 03 18 08 18 02 Confirm with lt START ENTERs
8. Surface calculation Derivative Compare spectrum Add spectrum Select Mark points with A and W keys Confirm with lt START ENTER gt key It displays Abs 0 855 Move the cursor along the curve with lt and gt keys Press on F4 key Mark to display the points coordinates 7440 s 0 038 NOTE When the operator uses directly Mark function he can mark all points on the curve without excep tion If the Mark function is used from the screen which displays spectrum minimum and maximum only those could be marked by the operator 178 Spectrum mode 9 7 5 3 Deletion of mark points 9 7 5 3 1 Deletion of individual mark points ee ee EE gt 740 S 0 038 Move the cursor along the curve with lt and gt keys When cursor arrives on marked point the Delete key appears beside Mark key Press F3 key Delete to remove the selected point 9 7 5 3 2 Deletion of all mark points lt HOME gt Spectrum Edit Delete all marks Press on F2 key Edit 03 26 08 16 30 Peaks amp valleys detection Mark points A Delete all marks Surface calculation lt Derivative Compare spectrum When there are marked points the command Delete all marks appears inside the Edit menu Select Delete all marks with A and Y keys Confirm with lt START ENTER gt key All marked points will be de
9. The original packing protects the photometer against damage if it has to be transported 5 2 First start up Perform the following activities Insert the buffer batteries see section 5 2 1 Connect the power supply see section 5 2 2 Switch on the photometer see section 5 2 3 Set the language see section 5 2 4 Set the date and time see section 5 2 5 VVVVV 5 2 1 Buffer batteries installation Fig 6 Buffer batteries setting place Instrument lower part sch Turn the photometer upside down and place it on a soft surface 2 Open the lid of the battery compartment 1 3 Insert the four batteries in the battery compartment Make sure that the poles of the batteries are in the correct position The signs on the batteries must correspond to the signs in the battery compartment 4 Close the lid of the battery compartment Batteries time life The power consumption of the clock is very low The lifetime of high quality batteries is at least 5 years 5 2 2 Connection of power supply The power is supplied via the enclosed plug in power pack The power pack supplies the photometer with low voltage 12 VDC 126 Start up Warning The line voltage of the usage location must fulfill the specifications stated on the power pack Always use the supplied 12 V original power pack only Abb 7 Connection of power supply 1 Connect the miniplug of the power pack to the socket 1 of the spectrophotometer 2 Connect the p
10. and Y keys Confirm with lt START ENTER gt key Edit Original values Mark paints Surface calculation Derivative NOTE The functions as Zoom zs Mark points Surface calculation and Derivative are also availa ble for a derivative curve A derivative curve can be also recorded in memory with lt STORE gt key Spectrum mode 181 9 7 5 6 Spectra comparison lt HOME gt Spectrum Edit Compare spectrum Load a second spectrum into the same diagram for direct comparison WARNING The comparisons of spectra can be done only on spectra which do not result from calculations de rivative multiplication Press F4 key Open and select the first spectrum Press F2 key Edit Vr Ir Edit Zi Peaks amp valleys detection Mark points 4 Surface calculation Derivative uka Compare spectrum Add spectrum Select Compare spectrum with A and W keys Confirm with lt START ENTER gt key Select the second spectrum and confirm with lt START ENTER gt key It displays 446 0 03 26 08 16 30 1 055 To come back to a normal screen first selected spectrum Press on F2 key Cd Select Original values with A and Y keys Confirm with lt START ENTER gt key 182 Spectrum mode 9 7 5 7 Addition of two spectra lt HOME gt Spectrum Edit Add spectrum Add a stored spectrum to the current spectrum Press F4 key Open
11. mode Spec dian Spectrum You have to record a baseline first lt ZERO BLANK Adjustment of wavelength range under lt Setup gt 168 Spectrum mode 9 2 Method programming lt HOME gt Spectrum Setup Press on F1 key Setup 03 26 08 14 56 Start wavelength Stop wavelength 1100 nm Mode Absorbance Smoothing Yes Scaling Auto If the operator doesn t change the acquisition parameters any more they will be by fault identical to the ones displayed above Start wavelength From 320 nm for the UviLine 9100 From 190 nm for the UviLine 9400 Stop wavelength Up to 1100 nm for both UviLine 9100 and UviLine 9400 Scan speed For UviLine 9400 only select Low Medium or High Mode Absorbance or Transmission Smoothing YES or NO Scaling Auto or Manual Auto The photometer adjusts the scaling of the axes minimum and maximum value of the axes to the measured values while measuring The entire curve is always visible Manual Abs min Abs max The scaling of the axes minimum and maximum value of the axes is permanently set manually Spectrum mode 169 9 3 Saving method lt HOME gt Spectrum Setup Parameters programming Save Location Enter in spectrum mode Press on F1 key Setup to enter inside the programming parameters sub menu Program parameters Press on F2 key Save Press on F1 key Location Select the pla
12. 188 Kinetics mode Edit method 2 of 2 04 09 08 16 12 Measurements interval 1 interval Katalytic activity Yes Factor Unit ukat Resolution 0 01 Factor Factor multiplying the average slope report to the minute Units i e ukat 10 characters maximum Resolution From 0 to 3 Number of digits after comma for the result Press F4 key Complete to leave the programming 10 3 Method saving Methods are automatically saved in memory 10 4 Method deletion lt HOME gt Kinetics Select method Setup Edit method Delete Select the method to be deleted with A and W keys Confirm with lt START ENTER gt key Zero measurement required 4001 NADH Absorbance Press F1 key Setup It displays Kinetics mode 189 0 08 8 14 New method Edit method Scaling Select Edit method with A and W keys Confirm with lt START ENTER gt key Edit method 1 of 2 Number Name NADH Mode Absorbance Wavelength 340 nm Duration 00 02 00 Interval 00 00 30 Delay 00 01 00 Scaling Auto Method Press F3 key Delete Answer Yes to the following message Delete method The method is then deleted 10 5 Method selection lt HOME gt Kinetics Select method all 04 10 08 8 23 pO 4001 NADH Absorbance 4002 TESTO1 Absorbance New Metho Last used The methods list is displayed The methods are classified by method numbe
13. 49 9 00 mg l NO3 03 12 08 9 49 9 00 mg NOZ 03 12 08 9 49 9 00 mg l NOZ Filter v Memory allocation 29 1000 03 18 08 16 41 Select the result to be deleted with A and W keys ra Hino ministr AssayO2 T w AutoStore a AuUtoStore a AUtoStore a AUtoStore F 03 18 08 16 46 Assay02 Autostore Assay01 Autostore 208 Memory Press F3 key Delete It will display Measurement data memory 03 18 08 16 50 03 12 08 10 57 gt 50 00 mgA NO3 Administr Assay02 03 12 08 10 57 gt 50 00 mol MO Administr AutoStore nt data 03 12 08 94 9 00 maA NO3 Autostore 03 12 08 949 9 00 mgA NOS ww AutoStore 03 12 08 2 49 9 00 mgA NO3 Administr AutoStore Filter e Memory alocation 29 1000 Select OK or Cancel 11 3 6 Copying a result file To backup files with measurement data outside the spectrophotometer it is possible to copy them to external media in different ways e Copying individual files on a USB memory e Copying of all files on a USB memory e Copying files on PC 11 3 6 1 Copying single files onto a flash drive 11 3 6 1 1 Spectrum and kinetic curve after measurement lt HOME gt Spectrum Kinetics Kinetic or spectrum acquisition Open Saving or not of spectrum or kinetics Location Select USB memory After measurements of spectrum or kinetics the user can save his curve spectral or kinetic in a csv file
14. Blank value 0 010 Std 1 10 00 mg l 0 198 Std 2 20 00 mg l 0 405 Std 3 30 00 mg l Enter with keyboard the absorbance values of the blank if necessary and the one of each standard Press F4 key Next 152 Concentration mode Concentration f x 0 02607 0 0353 R 0 5671 It displays then the calibration curve From this point the continuation proceeds as if the operator had measured the standards See paragraph 6 4 1 2 6 4 3 With a formula Edit method 03 19 08 15 01 c a0 al A az Af ail ap AT ad AP a 1 805 al 30 52 ER 2 15 as 0 149 ER a5 Lower limit of measuring range 0 00 mg l Upper limit of measuring range 75 00 mg l When the formula is entered press F4 key Next It displays Concentration 03 20 08 15 42 Zero measurement required 10 00 30 00 mg l From this point the continuation proceeds as if the operator had defined his calibration curve See para graph 6 4 1 6 Concentration mode 153 6 5 Method editing lt HOME gt Concentration Select method Setup Edit method Enter in Concentration mode Select a method with A and Y keys then confirm with lt START ENTER key Zero measurement required 10 00 30 00 mg l Press F1 key Setup It displays Concentration 03 20 08 15 50 Dilution Sample blank value Display absorbance New method Edit method Delete method Measurement data memory
15. average of the various measured values To measure the solution once again press F1 key Next meas Press lt START ENTERs gt key to measure the solution once again Remark The number of times that the operator can measure again the solution is not limited Blank value 03 19 08 16 02 Last measured absorbance 0 057 Median 0 055 2 Measurement s Nitrate The value will take in account is displayed under Median term Press then F4 key Apply It displays Blank value Apply Last measured absorbance Median Discard The operator can choose to preserve the last measured value by validating the option Last measured absorbance or to use the average value calculated by choosing and validating the option Median 140 Concentration mode It displays Concentration 03 19 08 16 09 Blank value Std 1 10 00 my Ei Std 2 20 00 mg l Std 3 30 00 mg l Install the first standard to be measured Press F3 key Measurement lt displays Std 1 10 00 mg l Start measurement with lt START ENTER gt Nitrate Start measurement of the first standard by pressing lt START ENTER gt key Std 1 10 00 mg l 03 19 08 16 13 Last measured absorbance 0 350 Median 0 350 1 Measurement s Nitrate As for the blank the operator can reject measurement F2 key Discard or make several times the measurement of the same standard F1 key Next meas and preserve th
16. gt key The entire folder structure of the spectrophotometer is created in the USB memory The individual files with measurement data are in subfolders separated by the type of measurement data 11 3 6 3 Exportation data onto a PC It is possible to copy measurement data to a PC after they have been stored in csv format Measurement data in csv format can be directly imported and processed in spreadsheet programs such as Mi crosoft Excel Use a USB memory as a temporary storage see beginning of this paragraph then connect the USB memory to the PC on which it will be readable Remark Depending on the country variant some spreadsheet programs require a certain decimal separator for the cor rect import of numerical values comma or point The decimal separator can be selected in the following menu lt HOME gt gt General setup gt Data transfer Printer gt Decimal separator for csv files User management 211 12 User management There are a user name and a password by fault Username Administrator Password admin Respect the spaces and the letters size Capital or small letter The functions of the user management are only available for users of the user group Administrator An administrator can activate or deactivate the user management for the spectrophotometer create change or delete individual user accounts The spectrophotometers UviLine 9100 and UviLine 9400 allow the management of up to 100 use
17. j A T fe a H Dr Robert Gelder Gesch ftsf hrer Managing Director SI Analytics GmbH Hattenbergstrake 10 99122 Mainz Deutschland Germany Allemagne Typ type type tipo UviLine 9100 amp UviLine 9400 Bescheinigung des Herstellers Wir best tigen dass das oben genannte Ger t gem DIN EN ISO 9001 Absatz 8 2 4 berwachung und Messung des Produkts gepr ft wurde und dass die festgelegten Qualitatsanforderungen an das Produkt erfullt werden Supplier s Certificate We certify that the above equipment has been tested in accordance with DIN EN ISO 9001 Part 8 2 4 Monitoring and measurement of product and that the specified quality requirements for the product have been met Certificat du fournisseur Nous certifions que le produit a ete v rifi selon DIN EN ISO 9001 partie 8 2 4 Surveillance et mesure du produit et que les exigences sp cifi es pour le produit sont respect es Certificado del fabricante Certificamos que el aparato arriba mencionado ha sido controlado de acuerdo con la norma DIN EN ISO 9001 secci n 8 2 4 Seguimiento y medici n del producto y que cumple con los requisitos de calidad fijados para el mismo SI Analytics a xylem brand SI Analytics GmbH Hattenbergstr 10 Tel 49 0 6131 66 5111 Fax 49 0 6131 66 5001 55122 Mainz Deutschland Germany Allemagne Alemania E Mail support si analytics xyleminc com www si analytics com SI Analytics is a tradema
18. left corner of the zoom window small black square with lt and gt keys and with A and Y keys Use F4 xy max key to pass from the bottom left corner of the zoom window to the top right corner of the Zoom window 14 01 08 15 37 Define the top right corner of the zoom window small black square with lt and gt keys and with A and Y keys Scaling up the zoom window Press on lt START ENTER gt key The zoom window is scaled up on the entire diagram area 5 E 03 26 08 16 30 A 536 0 Abs 0 176 Leaving the zoom view Press on lt ESC gt key to come back to the spectrum original view Spectrum mode 175 9 7 4 Manual change of absorbance scale lt HOME gt Spectrum Open Setup Scaling Manual Press on F1 key Setup select Scaling and then Manual to determine manually the absorbance scale Y axis Enter manually the minimum and the maximum of absorbance 9 7 5 Calculations 9 7 5 1 Peaks and valleys detection lt HOME gt Spectrum Edit Peaks amp valleys detection Press on F2 key Cd of Edit Peaks amp valleys detection Mark points A Surface calculation Derivative Compare spectrum Add spectrum Select Peaks amp valleys detection with A and Y keys Confirm with lt START ENTER gt key It displays Automatic Manual input Automatic Maxi
19. menu tree with the relevant operating steps lt HOME gt Bold letters and angle brackets indicate a Configuration key on the photometer except function Langague keys Press the lt Home gt key The main menu is called up Square brackets indicate a function key F1 to F4 The text between the brackets corresponds to the assignment according to the function key menu on the bottom edge of the display Press the function key with the assignment i e Setup The corresponding menu appears Text without square brackets indicates a menu item indicated on the display list item Select the menu item with the arrow keys A and W The current selection is displayed in reverse video Then press lt START ENTER gt Other possibilities of navigation e The lt ESC gt key moves the operator one level up in the menu tree e The lt HOMEs gt key directly calls up the main menu Remark If the operator is lost in a menu he has to press lt HOME gt key and restart navigating from the main menu 120 Overview 4 Overview 4 1 Overview of the device Fig 1 Front of the meter with control elements 1 LCD graphic screen 2 Membrane keyboard 3 Cell compartment 4 Cover of the cell compartment Overview Fig 2 Back of the meter with the interfaces 5 Connection for power supply 6 RS232C plug 7 USB A plug 8 USB B plug 4 2 Keyboard 4 2 1 Overview 1 2 Fi F2 F
20. necessary to put it out of service and to protect it from involuntary starting up again Use without danger will not be possible when the spectrophotometer e suffered damage during transportation e was stored under inadequate conditions for a relatively long period e shows some visible damages e does not function as described in the user s manual anymore In case of doubt consult the spectrophotometer supplier General 119 3 General 3 1 About UviLine Spectrophotometers The main differences between UviLine 9100 and UviLine 9400 spectrophotometers are the wavelengths ranges and the light sources UviLine 9100 Wavelengths range 320 1100 nm Light source Halogen lamp 5VDC 10W UviLine 9400 Wavelengths range 190 1100 nm Light source Xenon lamp The software for UviLine 9100 and UviLine 9400 is identical only the wavelengths ranges are different see above For this reason the user s manuals are the same for both instruments except chapter about lamp change 3 2 About navigation inside the user s manual In this operating manual the introductory navigation steps leading to individual menus or dialogs are clearly shown in a gray box The box indicates a section of the menu tree Starting point of the description is always the main menu which can be reached with the lt HOME gt key from any operating situation From there navigation takes place downward The following example shows the elements of the
21. or commercial reasons or in the necessity to comply with the statuary stipulations of various countries SI Analytics may perform additions to the Spectral Photometers UviLine 9100 and UviLine 9400 without changing the described properties SI Analytics TABLE OF CONTENT PAGE 1 10 ST scicate dace wate teceseewe cevieec a aeaa aaaea aaa 117 Tet ut tel tte el EE 117 Warning and Safety instructions cccceeeeeeteneeeeeneeeeeneeeeeneeeeaneeeeeneeeeaneeenenenees 118 Eegeregie 119 3 1 About UviLine Spectrophotometers EE 119 3 2 About navigation inside the user s manual 119 EE Wica D nee Eee ner nie Mery Een ene ERC mnE rrr erry ep eee ten ener rer eres ener ener sre r ere spree rte 120 Al Overview HEEM 120 Geen EIERE eege 121 SC EE EE 124 SE ams Ee El le 124 5 SLANE ENEE 125 Del PACKAGING EE 125 Die INSU SIAN re DEE 125 Belle le dere ur e Le TEE 131 6T det eeh eieiei ul e BEE 131 De MEO SIOl Le ernst ranteninaintinicabinicedee nice sinkcrdichinintaealeaawanaalsanaeaaazaaaaaa senna sansesaatmaen inde dadnendhaastaeduindes 136 6 3 Methode deletion gegegegegegegegegegegeeegee eege cae anes 136 oA Metroda AN OM D 137 BiB det OON EE 153 Absorption Transmission Mode cccssccseeseeeseseeeseneeeeeseeeeeneeeeaseeeeaseeeeanens 154 Ce WM a 2 EE 154 1 2 EE EE 155 E SEET UNINC E 156 7A Oaving Tesu Ssss ate emit dba cat ae nan tan aba aaN sana baaNeadae 156 Multi wavelengths mode EE 157 Sal Meas rement principle E
22. parameters are entered by the operator in order to create methods which can be stored edited and deleted lt HOME gt Kinetics If necessary come back to the main menu with lt HOMEs gt key Select with A and Y keys Kinetics mode Absorbance Transmission Multi wavelengths Kinetics General setup Logout Enter in the kinetics mode Select method all 04 09 08 14 42 New Method 186 Kinetics mode 10 2 Method programming lt HOME gt Kinetics New method Press on F1 key New method It displays Edit method 1 of 2 Number Name Mode Wavelength Duration Interval Delay Scaling Delete Method Remark In kinetics mode methods are recorded under number from 4001 to 4020 Edit method 1 of 2 04 09 08 15 13 Number Name Mode Duration Interval Delay Scaling Number 4001 4020 Nam Name of the method 18 characters Mode Absorbance or Transmission Wavelength 320 1100 nm for l UviLine 9100 190 1100 nm for l UviLine 9400 Duration Delay Measure Wavelength 4001 NADH Absorbance 340 nm 00 02 00 00 00 30 Total duration of the kinetics including the waiting time Delay Format hh mm ss hours minutes seconds Interval It is the time interval between two successive single measurements Format hh mm ss hours minutes seconds Kinetics mode 187 Exception With the setting Measure
23. to add other standards Maximum 10 by pressing F2 key Add and to enter their concentra tion To delete a standard select it with A and W keys and press F3 key Delete Concentration 03 19 08 14 27 Standard ID Standard manufacturer Blank value Standard conc 1 10 0 mg l Standard conc 2 20 0 mg l Standard conc 3 30 0 mg l When all standards are entered press F4 key Next to continue ENTER COUPLES OF VALUES Number 1001 Wavelength 500 nm Resolution 0 1 Unit mg l Citation form NOS Designation Nitrate Version 0 9 75 3 mg L Calibration curve Enter couples of values Concentration mode 135 Press F4 key Next It displays Concentration 03 19 08 14 47 Standard ID Standard manufacturer Blank value Yes Standard conc 1 10 0 mg l Standard conc 2 20 0 mg l Standard conc 3 For the choice of the blank and the possibility to add some standards proceed as before When the standards are entered press F4 key Next ENTER FORMULA Concentration H 03 19 08 14 50 Number 1001 Wavelength 500 nm Resolution 0 1 Unit mg l Citation form NO3 Designation Nitrate Version 0 9 75 3 mg L Calibration curve Enter formula Press F4 key Next It displays Edit method 03 19 08 14 54 c al al A azr A az AT adAt ap AN a al E az a3 a4 ao Lower limit of measuring range Upper limit of measuring range Enter a function as C a5 A a4 A a3 A a2
24. to select USB memory as a source and press lt START ENTER gt key The update process takes approx three minutes The spectrophotometer switches itself off and then on again Note If the photometer does not correctly start after a software update e g no self test due to a power failure during the software update Press the lt F3 gt key and continue the software update Maintenance 221 14 Maintenance 14 1 Changing lamp In this chapter only the change of the halogen lamp of the UviLine 9100 spectrophotometer will be explained Indeed the UviLine 9400 spectrophotometer works with xenon lamp which life time approaches the one of the apparatus Any time if it prematurely stops working then contact the services of SI ANALYTICS for a change of this lamp The tungsten halogen lamp is a wear part with a certain average service life It has to be replaced if defective The spectrophotometer has a service hour counter for the lamp module see paragraph 13 6 Remark The replacement lamp is readily assembled as a lamp module and optically adjusted in the factory Therefore treat it with utmost care Fingerprints on the glass will shorten the service life of the lamp Do not touch the bulb of the new lamp module with bare fingers If you have touched the bulb inadvertently carefully clean it with a clean cloth soaked in alcohol The halogen lamp is located behind a protection cover Aluminum sheet at the rear part of the spectroph
25. 3 F4 l l A 1 2 3 p 4 5 D E 7 A o ESC 7 START ENTER l l 3 Fig 3 Keyboard 1 F1 to F4 function keys functions which depend on the menu 2 Fix function keys 3 Alphanumeric key block 121 PRINT STORE ZERO BLANK TIMER 122 4 2 2 Keys function Key O HOME PRINT STORE ZERO BLANK TIMER START e ENTER e Function keys F1 to F4 The function keys F1 to F4 have different functions depending on the operating situation The current Overview Designation lt ON OFF gt lt HOME gt lt PRINT gt lt STORE gt lt ZERO BLANK gt lt TIMER gt lt Esc gt lt START ENTER ee A or Vv gt ee d gt P Function Switch the spectrophotometer ON and OFF Switches to the main menu from any operating situation Actions that are not completed are canceled Downloads the displayed value to an interface Saves a displayed value or spectrum or kinetic curve Starts one of the following measurements de pending on the operating situation Zero adjustment Blank value measurement Baseline measurement Open Timer menu Cancels the running action Entries that have not yet been accepted are discarded Switches to the next higher menu level Starts an action e g measurement Opens a selected menu Confirms a selection or entry Moves the selection in menus and lists one posi tion up or down De
26. 5 Edit Menu 10 8 5 1 Slope lt HOME gt Kinetics Open Edit Slope Calculation of raw slope without factor Katalytic activity NO Press F3 key Edit Select Slope It displays Interval Slope A min Interval column It is the number of measurement interval Slope A min column It is the slope report to the minute per interval Time s column It is the time at the end of each interval Total slope It is the slope report to the minute of the straight regression line passing through various points of measure ment Calculation of the activity Katalytic activity YES Press on F3 key Edit Select Slope It displays Kinetics mode 195 10 147 ukat Interval Slope A min 0 942 0 882 0 588 14 353 Interval column It is the number of measurement interval Slope A min column It is the slope report to the minute per interval Time s column It is the time at the end of each interval Total slope It is the slope report to the minute of the straight regression line passing through various points of measure ment RESULT It is the slope report to the minute of the straight regression line passing through various points of measure ment multiplied by the factor and expressed with the resolution and unit selected by the operator 10 8 5 2 Printing activity Press on lt PRIN
27. 857 Meas range 0 00 30 00 moll 10 0 15 0 20 0 25 0 Concentration mg l NOS Meas range To set back the removed standard press F4 key Back Add the standard Enter its concentration Concentration 03 20 08 14 37 Blank value 0 053 Std 1 10 00 mg l Std 2 20 00 mg l Std 3 30 00 mg l Measure the concentration of this standard once again or enter its absorbance following the curve build ing mode selected 146 Concentration mode 6 4 1 4 Kalibrierungskurve ausdrucken Drucken Sie die Taste lt PRINT gt UviLine 9100 7449001 1 14 WILLY 1001 Nitrate 0 9 75 3 mg L 500 nm f x 0 03207x 0 0427 R2 0 9993 Ce d ssnsnsnrsnnn dessen nsnsnnsn sf asss nsnnsf asss snssssslssssssnsnssslsssnssnsnssne n D asss nrnnnnss snnsnsnrsnnn dessen ses snrnnnnsn d snnsnsnrsnnn dr nf pes nnsn sf ssssnnnnsnssfssssssnssssslssssssnsnssslssssssnsnssns D 25 0 30 0 0 0 4 0 10 0 15 0 20 0 Concentration mg l 6 4 1 5 Method saving See paragraph 8 2 6 4 1 6 Sample measurement Zero measurement required 1001 Nitrate Press lt ZERO BLANKs key to start the blank measurement It performs the zero and displays Start measurement with lt START ENTER 1001 Nitrate NOS 0 00 30 00 mol Concentration mode 147 Press lt START ENTER gt key to start sample measurement Concentration 03 20 08 14 52 TAD voi Absorbance 0 272 1001 Nitrate NOS 0 00 30 00 mg
28. A a1 A a0 WithC Concentration 136 Concentration mode A Absorbance a0 to a5 free factors from 0 000 to 9999 000 Example Use of a K factor K C a1A e a0 0 e ail K factor e a2 a3 a4 ab Zero Example straight line regression C a1A a0 e a0 bias e al slope e a2 a3 a4 ab Zero Example third degree curve Nitrate test in sea water C 0 1493A 2 5154A 36 524A 1 865 a0 1 865 al 36 524 a2 2 5154 a3 0 1493 a4 amp ad 0 Edit method 03 19 08 15 01 c a0 al A a A ail ap AT ap A7 1 865 36 52 2015 0 149 Lower limit of measuring range 0 00 mg l Upper limit of measuring range 75 00 mg l Lower and upper limit of measuring range Choice between zero and the maximum value of measured parameter When the formula is entered press F4 key Next 6 2 Method storage The methods are automatically stored under the classification name from 1001 to 1100 Remark However methods using standards will be stored only after standards measurement 6 3 Method deletion lt HOME gt Concentration Select method Setup Delete method Select method to be deleted with A and W keys and press lt START ENTER gt key Concentration mode 137 Concentration 03 19 08 15 33 Zero measurement required 0 00 100 00 mg l Press F1 key Setup It displays Concentration 03 19 08 15 34 Dilution Sample blank value Display absorba
29. A10 are absorbances read at different wavelengths The operator must judiciously choose the value of the factors a0 a1 a2 a10 and the value of the factors bO b1 b2 b10 in such way that the general equation Equation 1 is identified with calculation to carry out See the examples hereafter 8 2 Calculations Determination of DNA purity index DNA purity index is given by the ratio of absorbance read at 260 nm on the absorbance read at 280 nm with elimination of a cloudy part of the solution by a measurement at 320 nm Equation 2 R ADS y60nm AOS 330nm ADS gan AOS 320nm To convert the general equation 1 in specific equation 2 the operator has to make the following program ming Weder mmm At Abs 50mm ann 0 Wavelenstn2 290m a2 Abs 200mm a2 o be 1 Weder 200m a3 epes a8 1 w A Wegen um Jalo w Wavelengths Ri fw Wavelength wl fw o o Wavelengin7 a a o j w o Wavelengins wl fw o w o Wavelengno aj fa o w o Wavelengin10 al Jain wo In Warburg Christian formula for proteins quantification 158 Multi wavelengths mode Equation 3 Cpr oreins 95 x ADS og 6pm 0 575 x ADS 260m given in mg mL To convert the general equation 1 in specific equation 3 the operator has to make the following program ming m ow mom a vae o vave oo F w ojJ wa Wuere mm 8 mamen ar tas o 0 Wa
30. E 157 EE ee e Te EE 157 Bude IWIS TMOG OL OCI ANIMA E 159 oA ele e Welle e EE 161 e 2411810 0 CUa meet cee meee en ier nr eric ne ee 161 90 ME NOTS e EE 163 0x Melhodaopicaloikasisini sa E a ren rere 164 st PANO SON SE EN 166 E RN ele e Ke gt e EE 166 SEELEN 167 CN Gene eee ee a a a ese 167 9 2 Method Programming TEE 168 93 SAVN MeO eere erences cs nwiel wis ode a eae ade aadoaneocaanaaenanendanne 169 OA Jee 169 OID WIEINOG SCISCHOM DEE 170 9 6 Measurement application 171 OF EIERE HEITE geed 173 le un ee EE 185 TON EE 185 10 2 Method programin EE 186 TOS WIGTHOG SIVO EE 188 1O04 Method CCIE el EE 188 MOUS Method Ee EE 189 SI Analytics 10 6 Method applcaton 10 7 Method editing cccccccceeecseecceeeceeeceeeceeeeeeseuseeeesaeeeneeens 10 8 Kinetics treatment cccccccceccseeceeeseeeeeeceeeeeeceeseeeeseeeeenees 10 9 Printing e TICE T1 dt le E tei EES VIEW ed T2 E Mel E e EE 11 8 Management of memorized results 12 User Manageme nt cccccsccesseceeseeceseeesesenseseneesensenes 12 1 JEE EE EE 12 2 Detalls e RTE en EE 12 3 Activating deactivating user management 12 4 Creating editing and deleting a user account 13 Various adjustments cceceeeeeeeeeeeeeseeeeeeeeesenseeeeneees CN Language Selec hON EE 13 2 Setting date and iM E nener ERANS T339 AGIOSIING CONIA E 134 RESO EE 13 5 Lamp servicing count down 13 6 Device mmtormaton t
31. EC DECLARATION OF CONFORMITY CE DECLARATION DE CONFORMITE Wir erklaren in alleiniger Verantwortung dass die folgenden Produkte Spektrophotometer UviLine 9100 UviLine 9400 auf das sich diese Erklarung bezieht Ubereinstimmt mit den folgenden EG Richtlinien EMV EG Richtlinie 2004 108 EG Sicherheit EG Richtlinie 2006 95 EG Angewandte harmonisierte Normen oder normative Dokumente EMV EN 61326 1 2006 EN 6100 3 2 2000 A2 2005 EN 61000 3 3 1995 A2 2005 Sicherheit EN 61010 1 2001 Mainz den 01 04 2009 Konf No Spectro 001a We declare under our sole responsibility that the following products Spectrophotometer UviLine 9100 UviLine 9400 to which this declaration relates are is in conformity with the following EC directives EMC EC Directrive 2004 108 EC Safety EC Directrive 2006 95 EC Applied harmonized standards or normative documents EMC EN 61326 1 2006 EN 6100 3 2 2000 A2 2005 EN 61000 3 3 1995 A2 2005 Safety EN 61010 1 2001 Nous d clarons sous notre responsabilit que les produit Spectrophotometre UviLine 9100 UviLine 9400 auquel se r f re cette d claration est conforme directives CE soul vantes CEM CE Directive 2004 108 EC S curit CE Directive 2006 95 EC Normes harmonis es ou documents normative appliqu es CEM EN 61326 1 2006 EN 6100 3 2 2000 A2 2005 EN 61000 3 3 1995 A2 2005 S curit EN 61010 1 2001 d E F ri si d S oO IO vs GR
32. OPERATING INSTRUCTIONS UviLine 9100 9400 SPECTROPHOTOMETER SI Analytics a xylem brand E erer SAMI Er in e WEE Seite 3 114 Wichtige Hinweise Die Gebrauchsanleitung vor der ersten Inbetrieonahme sorgf ltig lesen und beachten Aus Sicherheitsgr nden bitte die Spektralphotometer UviLine 9100 und UviLine 9400 ausschlie lich f r die in dieser Gebrauchsanleitung beschriebenen Zwecke einsetzen Beachten Sie auch die Gebrauchsanleitungen f r die mitzuverwendenden Ger te Alle in dieser Gebrauchsanleitung enthaltenen Angaben sind zum Zeit punkt der Drucklegung g ltig Es k nnen jedoch durch SI Analytics sowohl aus technischen oder kaufm nni schen Gr nden als auch aus der Notwendigkeit heraus gesetzliche Bestimmungen anderer L nder zu be r cksichtigen Erg nzungen an den Spektralphotometern UviLine 9100 und UviLine 9400 vorgenommen wer den ohne dass die beschriebenen Eigenschaften beeinflusst werden Operan ut iere CN Page 115 226 Important notes Read and observe these operating instructions carefully before using the Spectral Pho tometers UviLine 9100 and UviLine 9400 For safety reasons the Spectral Photometers UviLine 9100 and UviLine 9400 may only be used for the purpose described in these operating instructions Please also ob serve the operating instructions for the units to be connected All specifications in this instruction manual are guidance values which are valid at the time of printing However for technical
33. Perform the zero again Erroneous blank value Measure the blank again 226 Connecting accessories 15 Connecting accessories 15 1 Communication interface 1 Fig 11 Back of the instrument with connections 1 RS232C connection 2 USB A connection 3 USB B connection 15 1 1 RS232C port Connect the RS232C interface with the instruments as follows e PC with a commercially available zero modem cable e Printer with a commercially available RS232 printer cable The cables are available in specialized computer shops Set up the following interface data at the PC printer Baud rate 1200 2400 4800 9600 19200 The spectrophotometer baud rate must be the same as the one programmed on the used PC Printer Flow control Handshake None Parity None Data bits 8 Stop bit 1 With lt PRINT gt key data are sent to the RS232C interface e Ifa printer is connected data are printed e If acomputer is connected data can be received using a terminal program see paragraph 11 3 2 3 15 1 2 USB A port The USB A port allows e To print data when a printer is connected With lt PRINT gt key data are sent on the printer e To records data on USB memory USB key when it is connected e To update spectrophotometer software and methods data e To connect an external USB keyboard 15 1 3 USB B port Allows a direct connection between spectrophotometer and PC S Analytics EG KONFORMITATSERKLARUNG
34. T gt key UviLine 9100 7449001 1 18 Willy 04 10 2008 14 05 46 4001 TESTO1 Katalytic activity 8 754 ukat Interval Slope A min Time s 1 0 294 30 s 2 0 942 60 s 3 0 882 90s 4 0 588 120s Total slope mn 1 353 120s 10 8 5 3 Kinetics comparison Load a second kinetics curve into the same diagram for direct comparison Press on F3 key Edit Select Compare kinetics Select another kinetics curve Confirm with lt START ENTER gt key It displays 196 Kinetics mode 4001 MADH Number of measurements 3 Interval 00 00 30 To come back to a normal screen first selected kinetics curve Press on F1 key Back 10 8 5 4 Subtraction of a kinetic curve from one another lt HOME gt Kinetics Select method Open Edit Subtract kinetics Subtract a stored kinetics curve from the current kinetics curve Press on F3 key Edit Select Subtract kinetics Select a kinetics curve Confirm with lt START ENTER gt key The curve resulting of this operation can t be saved in memory To come back to a normal screen first selected kinetics curve Press on F1 key Back WARNING To subtract one kinetics curve from another one the intervals have to be identical 10 8 6 Manual change of absorbance scale lt HOME gt Kinetics Select method Open Select a kinetics curve Setup Scaling Manual Press on F1 key Setup select Scaling and th
35. Unit Resolution 2001 PROT 1 0 Proteines mg mL 0 1 Method list Press F3 key Delete Answer YES to the message Delete method The method will be deleted Multi wavelengths mode 163 8 6 Method selection lt HOME gt Multi wavelengths Select method Select method all 03 25 08 16 17 ee 2001 PROT Proteins mag imL 2002 DNA DNA Purity New Method The method list is displayed Methods are ordered by method number Method selection Select a method with A and VW keys The active selection is displayed in reverse video Accept the selection with lt START ENTER gt key The spectrophotometer is ready to measure 8 6 1 Limitation of methods list If the list is very long the operator can narrow down the method list and thus make the search easier as fol lows e Press F4 key Last used it is possible to restrict the method list to the ten methods last used e With the search function the operator can search certain character strings in the list The search takes place as a full text search of the entire list contents The operator can search for a method number or certain citation form 8 6 2 Research function Search for a character string Enter the character string to be searched for in the search window with lt A 9 gt The list appearing below shows all hits containing the character string The hit list is updated with each charac ter that is entered
36. YO4 03 18 08 15 19 gt 50 00 mgA NO3 ASSAYO3 W W W 03 18 08 15 19 gt 50 00 ma NO3 w AutoStore Filter e M Soasi allocation E 1000 Single Select the result to be displayed with A and W keys Press F2 key Single value It will display 206 Memory Measurement data memory 03 18 08 16 15 30 3 18 2008 16 14 11 w AutoStore 1001 16 98 mg INO3 1 2 Abs 0 056 Fitter w Memory allocation 29 1000 Following data are displayed memorized sequence number of the result measurement date measurement time operator name storage setting analyze name parameter concentration citation form dilution rate absorbance Press on F2 key List to come back to all results 11 3 5 Deletion of results There are several ways to delete the results 11 3 5 1 Deletion of selected single results lt HOME gt Concentration Absorption transmission or Multi longueurs d onde Setup or General setup from main menu Measured value memory Setup Delete memory selected values only It will delete only results corresponding to the normal or invert selection criteria Select Delete memory selected values only with A and W keys and confirm with lt START ENTER gt key It will display Measurement data memory 03 18 08 16 35 03 18 08 16 14 16 98mq NO3 w AutoStore 03 18 08 16 13 1094 bb mol NO3 w Autostore 7 oes D bi
37. active Not active Not active Not active Not active Set the filter criteria Press F1 key Reset entry if necessary to deactivate any selected filter criteria Press F4 key Apply to confirm the filter selection The Measurement data memory list is displayed Measurement data m 11 08 10 03 03 19 08 15 29 10 00 mg l AutoStore 03 19 08 15 16 10 00 mg l AutoStore 03 18 08 16 14 16 98mq NO3 AutoStore 03 18 08 16 13 104 55mq NO3 AutoStore 03 18 08 16 13 70 89mq NO3 AutoStore 03 18 08 15 22 1 00 A1fA2 i ASSAYO4 03 18 08 15 19 gt 50 00 mgA NO3 ASSAYO3 03 18 08 15 19 gt 50 00 mgA NO3 AutoStore 03 12 08 10 57 gt 50 00 emol MO Administr Assay02 03 12 08 10 57 gt 50 00 emol MO Administr AutoStore 03 12 08 10 56 20 40 mgANO3 Administr Assa Her v Memory allocation 39 1000 Setup Single value The following information is displayed additionally Current memory allocation Active filter criteria Filter Remark Alternatively it is possible to hide measurement datasets that meet the specified filter criteria with the Selected values invert selection function see paragraph 11 3 4 2 Memory 205 11 3 4 2 Filters inversion lt HOME gt Concentration Absorption transmission or Multi longueurs d onde Setup or General setup from main menu Measured value memory Setup Selected values invert selection With the Selected values invert selection
38. and select the first soectrum or use the acquired spectrum Press F2 key Edit aaj Ir Edit Peaks amp valleys detection Mark points Surface calculation Derivative e Compare spectrum Add spectrum Select Add spectrum with A and W keys Confirm with lt START ENTER gt key Select the second spectrum and confirm with lt START ENTER gt key It displays the summary of the two spectra To come back to a normal screen first selected spectrum Press on F2 key Cd Select Original values with A and Y keys Confirm with lt START ENTER gt key 9 7 5 8 Subtraction of one spectrum from another one lt HOME gt Spectrum Edit Subtract spectrum Subtract a stored spectrum from the current spectrum Press F4 key Open and select the first spectrum or use the acquired spectrum Press F2 key Edit Mark points Surface calculation Derivative Compare spectrum Add spectrum Select Subtract spectrum with A and Y keys Confirm with lt START ENTER gt key Spectrum mode 183 Select the second spectrum and confirm with lt START ENTER gt key It displays the difference of the two spectra To come back to a normal screen first selected spectrum Press on F2 key Cd Select Original values with A and Y keys Confirm with lt START ENTER gt key 9 7 5 9 Division of a spectrum lt HOME gt Spectrum Ed
39. asurement with lt START ENTER gt nim If there are several measurements the last four are displayed at the same time as the measurement in progress Press F4 key Continuous to measure the same sample several time again The measurement is about every 2 seconds 03 25 08 14 23 0 340 45 7 0 340 45 7 Yo 0 340 45 7 Yo 0 340 45 7 Yo 0 340 45 7 Yo Continuous mode Press F4 key Stop to stop the continuous measurement 156 Absorption Transmission mode 7 3 Printing results Press lt PRINT gt key to print the results UviLine 9100 7449001 1 14 Administrator Time of measurement 07 01 2008 14 05 46 Wavelength 925 nm Measured value 0 27852 8 7 4 Saving results lt HOME gt Absorption transmission Setup or General setup from the main menu Measurement data memory See paragraph 11 3 and especially paragraph 11 3 3 Multi wavelengths mode 157 8 Multi wavelengths mode 8 1 Measurement principle This measuring mode allows making different calculations on a sample measured at several wavelengths ra tio Allen correction It also makes it possible to have the absorbance value of a solution read at several wavelengths up to 10 The general calculation is Equation 1 aQ al Al a2 A2 4 al0 Al0 b0 b1 A1 b2 A2 B10A10 R is the result of the calculation a0 al a2 a10 b0 b1 b2 b10 are factors which allow calculations on the absorbances Al A2
40. aw values A 260 nm 0 586 A 280 nm 0 572 8 9 Method editing lt HOME gt Multi wavelengths Select method Setup Edit method Enter in the Multi wavelengths mode Select a method with A and W keys then confirm with lt START ENTER gt key Multi wavelengths 03 26 08 14 23 Zero measurement required Setup Method list Transmission Press F1 key Setup It displays Edit method Measurement data memory Select Edit method with A and W keys then confirm with lt START ENTER gt key Spectrum mode 167 9 Spectrum mode 9 1 General The Spectrum mode makes it possible to measure and record the Absorption and Transmission values accord ing to the wavelength The wavelength range can be freely selected inside the spectrophotometer measuring range The measurement step is 1 nm Baseline A baseline has to be recorded before a spectrum is recorded The baseline has to cover at least the wave length range of the spectrum to be recorded Once the baseline is measured it remains stored in the photome ter until a new baseline is recorded the Spectrum mode is exited or the photometer is switched off lt HOME gt Spectrum If necessary come back to the main menu with lt HOMEs gt key Select with A and W keys Spectrum mode Absorbance Transmission Multi wavelengths Spectrum General setup Enter in the Spectrum
41. ble to select the spectrophotometer nternal DataB folder or a connected USB memory at the USB A connection USB memory Stored spectra can be recalled and edited at any time see paragraph 9 7 1 9 7 Spectrum editing 9 7 1 Loading saved spectrum lt HOME gt Spectrum Open Press on F4 key Open Open Internal DataB folder 03 26 08 16 21 03 26 08 TEST 112 csyv 03 26 08 TEST 111 csv 03 18 08 Spectrum_320_1100_080318_1657 csv Location Stored spectra list is displayed e If necessary it is possible to select a different memory location for the spectrum with F1 key Location USB medium at the USB A connection e Select the required spectrum The original view of the curve is displayed Location Delete 9 7 2 Cursor 03 26 08 16 30 ue 536 0 e Information cursor Abs 0 176 The cursor consists of a horizontal and vertical line that crosses each other at a point of the curve A box names the x and y values of the point of the curve Move the cursor along the x axis wavelength with lt and gt keys It is possible to trace and evaluate the curve point by point 174 Spectrum mode 9 7 3 Zoom Press on F3 key Zoom 03 26 08 16 40 The zoom window appears Press F1 key Original at any time to come back to the original view of the spectrum The bottom left corner of the zoom window is marked by a small black square Adjusting the zoom window Define the bottom
42. ce where the method will store Internal folder DataB or USB memory Give a name to the method and confirm with lt START ENTER gt key 9 4 Method deletion lt HOME gt Spectrum Setup Open Select method Delete Enter in the Spectrum mode 03 26 08 14 54 Spectrum You have to record a baseline first lt ZERO BLANK gt Adjustment of wavelength range under lt Setup gt T E Press on F1 key Setup It displays Start wavelength 320 nm Stop wavelength Mode Smoothing Scaling Press on F1 key Open It displays 170 Spectrum mode Open Internal DataB folder 03 26 08 15 25 el ka 03 26 08 ASSAYO1 profil 03 26 08 TEST profil Select the method to be deleted with A and W keys Press on F2 key Delete The method is deleted 9 5 Method selection lt HOME gt Spectrum Setup Open Spectrum l 03 26 08 14 56 Start wavelength 320 nm Stop wavelength 1100 nm Mode Absorbance Smoothing Yes Scaling Auto Enter in spectrum mode Press on F1 key Setup to enter inside the programming parameters sub menu Press on F1 key Open Press on F1 key Location if the method is stored inside a USB memory Select the method and confirm with lt START ENTER gt key Spectrum mode 9 6 Measurement application lt HOME gt Spectrum 03 26 08 14 54 Spectrum You have to record a baseline first lt ZERO BLANK A
43. dards con centration F2 key Curve type It allows changing the curve calculation mode Curve type Straight line Linear regression Quadratic regression Straight line through 0 Linear regression through 0 Quadratic regression through 0 Straight line The straight line calibration curve is consisted of line segments connecting the calibration points between them Linear regression Curve whose equation is form ax B Quadratic regression Curve which equation is ax bx C Curves through 0 The calculation of these curves takes in account Item 0 Select the type of curve with A and W keys and confirm with lt START ENTER key The software will redraw the curve taking in account of the new calculation mode The equation of the calculated curve is displayed as well as the coefficient of R determination Concentration mode 143 f x O 03207 0 0427 R 0 9993 Meas range 0 00 30 00 mg l eee ee ee ee ee ee ee eee ee eee eee ee F3 key Meas range It allows to the operator to reduce the parameter measuring range Lower limit mg l Lower limit entered by the operator Upper limit entered by the operator Upp er limit These limits are materialized by an arrow W on the concentration axis Concentration 03 20 08 14 24 f x O 03207x 0 0427 Mess range 10 00 30 00 mg l Any value under or above limits fixed by the operator will be declare as gt or lt
44. djustment of wavelength range under lt Setup gt A message which contains instructions to follow is displayed Press on F1 key Setup Start wavelength 320 nm Stop wavelength 1100 nm Mode Absorbance Smoothing Yes Scaling Auto Open Enter the method parameters see paragraph 9 2 Press on F4 key Apply to pass the following step Spectrum You have to record a baseline first lt ZERO BLANK gt Adjustment of wavelength range under lt Setup gt Start the baseline measurement by pressing lt ZERO BLANKs gt key 171 172 Spectrum mode The spectrophotometer memorizes the baseline Wait until the end of measurement It displays Spectrum Start measurement with lt START ENTER gt Insert the sample inside the cuvette holder Close the cover Press lt START ENTERS key to start the measurement When the spectrum is finished it displays the following message Spectrum Recording of spectrum is completed Press lt START ENTER gt key to confirm Information curseur The cursor appears at the absolute maximum of the spectrum Spectrum mode 173 The operator has the following possibilities Immediately realized some calculations on the spectrum See paragraph 9 7 To print with lt PRINT gt key the spectrum on a connected printer as a graphic To record with lt STOREs gt key the spectrum as a csv file As the memory location it is possi
45. e average value Make in the same way the measurement of the other standards After the standards measurement it displays Concentration mode 141 Concentration 03 19 08 16 20 Blank value Std 1 10 00 mg l Std 2 20 00 mg l Std 3 30 00 mg l Press on F4 key Next NOTE If the programming of standards concentration is not increasing or decreasing in a monotonous way or if the measurement of the blank and standards absorbances is not also increasing or decreasing in a mo notonous way see example below the spectrophotometer will display an error prompt see below Concentration 03 19 08 16 20 Concentration 03 19 08 16 20 Blank value 0 055 Blank value Std 1 10 00 moll 0 350 Std 1 10 00 mol Std 2 20 00 mg l 0 680 Std 2 20 00 mg l Std 3 30 00 mg l Std 3 30 00 mg l Measurement Increasing and monotonous absorbances No monotonous absorbances The following message will appear Invalid value Couples of values must rise or fall strictly monotonously OK 142 Concentration mode 6 4 1 2 Calibration curve displaying f x De 0 0427 Mess range 0 00 30 00 mg l Meas range F1 key Back It allows to the user in the event of error to return back to the analysis programming and to modify the acquiring way of the values to make the curve standards measurement use of the curve equation or entry of the Concentration absorbance couples That also enables him to change the stan
46. e modes can be re imported and viewed on the photometer As the location for the PC readable files csv to be stored it is possible to select for each export either the spectrophotometer Internal DataB folder or an external memory USB memory Later it is possible to transmit the files stored in the photometer Internal DataB folder to a connected PC or an external memory USB memory It is possible to transmit to a USB memory either individual files with measuring data or all files stored in the Internal DataB folder 11 2 Memorized results 11 2 1 Composition A complete measurement dataset consists of Consecutive number is automatically assigned by the spectrophotometer Date time Identification e g ID or AutoStore User name Measured parameter e g method number dilution wavelength depending on the measuring mode Measured value with unit and if necessary citation form 11 2 2 Operation on memorized results Measurement datasets can be Stored see paragraph 11 3 3 Displayed and printed see paragraph 11 3 1 Filtered i e selected or hidden based on certain criteria See paragraph 11 3 4 Deleted see paragraph 11 3 5 Copied see paragraph 11 3 6 200 Memory 11 3 Management of memorized results 11 3 1 Displaying results From main menu lt HOME gt General setup Measured value memory From concentration menu lt HOME gt Concentration Choice of a mem
47. e time count down Press F2 key Stop to stop the time count down When the programmed delay is ended e The timer displays 00 00 00 e Its status commutes from Running to Expired and e One can hear a bip e Press F2 key Stop to deactivate the timer 220 Various adjustments 13 8 Software update The software and method update is used to continuously update the spectrophotometer Remark Only users of the user group Administrator are allowed to carry out a software and method update The update comprises e The newest firmware meter software e New or changed method data Data transfer to the spectrophotometer can be made with USB medium as a temporary storage To realize the update the new software version or new methods have to be recording on USB medium This one has to be connected to the spectrophotometer Execution 1 Connect the USB medium to the PC 2 Unpack the contents and complete folder structure of the downloaded exe or zip file in the main directory top level of the USB medium Remark If an unpacking program such as WinZip is used the option Nutze Ordnernamen or Use Folder Names must be set 3 Connect the USB medium to the spectrophotometer 4 Switch on the spectrophotometer if necessary lt HOME gt General setup Software update Software update 03 11 08 7 31 Software update Select source of update data USB memory PC 5 Press A and W keys
48. e transporting of the equipment in packaging which is not its original packag ing The lamps the cells and generally any item which appears in the accessories section on the price list 118 Warning and safety instructions 2 Warning and safety instructions WALINEIWO Always make sure that the instrument is connected on the good voltage Between 100 240V 50 60Hz Always disconnect the mains plug before starting any work inside the instrument When substances dangerous for health and environment are used the laboratory or site rules where the instrument is installed must be followed Take all the necessary precautions during the use the instrument to protect the operator from eventual liquids leaks or spills or possible radiations protective gloves anti UV ra diation glasses protected clothes etc Keep the sample compartment clean The Xenon lamp used in the UviLine 9400 emits UV radiation Install the instrument in a ventilated area because it is likely to generate ozone which be yond the limits below can harm health Exposure average value 100 ppb Exposure limits value 200 ppb All operations made inside the instrument must be done by SI Analytics or by SI Analyt ics authorized technicians Using spectrophotometer without danger Use of the spectrophotometer without danger If it is necessary to suppose that it is not possible any more to use the spectrophotometer with out danger it is
49. en Manual to determine manually the absorbances scales Y axis Enter the absorbance minimum and maximum Kinetics mode 10 9 Printing curve Press lt PRINT gt key to print the curve on a connected printer UviLine 9100 7449001 1 14 Administrator 04 10 2008 14 05 46 4003 CC SS aas n San E SS RSR SS a ban eb RSR RSR RSR RSR BapRRRSss 197 198 Memory 11 Memory 11 1 General view Concentration pec LIM Absorbance ix Transmission Kinetics Multi wavelengths lt STORE gt for AutoStore s Measured 1 lt STORE gt lt STORE gt Filter optional Ks Open in source application PC 4 opectrall ranster Internal folder D DataB Copy all files Ce e ks Memory 199 Measurement data Saving and exporting Concentration Measurement datasets of these modes are saved with Absorption transmission lt STORE gt or AutoStore in the measured value memory of the spectrophotometer first 1000 locations Multi wavelengths The measured value memory is available via the Meas urement data memory menu Here it is possible to view or filter stored measurement datasets or export them into a PC readable file csv lt STORE gt Csv files of these modes cannot be imported back into the photometer Spectrum Measurement datasets of these modes are saved and Kinetics exported as a PC readable file csv with lt STORE gt Csv files of thes
50. er management 12 4 2 Editing a user account lt HOME gt General setup User management Change At the time of the modification of a user account it is possible to modify the membership of User group and the Password Only the user having an Administrator account is authorized to make these changes Name User group Admir bk Change Change 1 Select a user account 2 Press F4 key Change to modify the user account The selection field for the user group Administrator User opens up 3 If necessary select and confirm another user group The input field for the password opens up 4 If necessary enter lt A 9 gt and confirm another password The user account is changed and appears in the list of user accounts 12 4 3 Changing password lt HOME gt General setup User management Change password The administrator creates user accounts and assigns a password on each user account As soon as a user opened a session successfully with his user account it can modify itself the password of his user account User management 03 12 08 9 15 Old password 1 Enter the previous password and confirm 2 Enter new password and confirm The password is modified User management 215 12 4 4 Deleting a user account lt HOME gt General setup User management Delete 1 Select a user account 2 Press F3 key Delete to delete the user account A security prompt appears 3 Co
51. ethod list Enter here general data of the method The next number of method available is already registered as next number of analysis to be used 160 Multi wavelengths mode To fill the fields with entry it is possible to proceed as follows Fill the entire empty fields with entry ones after the others Press the key F2 Method list to select a method already recorded Press the F3 key Delete to remove the method completely Press the F4 key Nex to confirm all the entries and to pass in the following page Edit method 2 of 6 03 25 08 15 39 Wavelength 1 280 nm Wavelength 2 260 nm Add Press F2 key Add to add another wavelength Press F3 key Effacer to delete the last wavelength Press F4 key Next to confirm all the entries and to pass in the following page Edit method 3 of 6 03 25 08 15 44 a0 al Al a A a10 Alo b bi Al b2 A b10 Ald 0 000 0 000 1 150 i 0 000 0 000 0 000 J 0 000 0 000 0 000 0 000 Enter the factors value for more information about the equation programming see paragraph 8 2 Press on the F4 key Next to confirm all the entries and to pass in the following page bO b1 Al b2 A2 4b10 A10 bo b6 0 000 b1 0 000 b7 0 000 b2 0 000 bs 0 000 b3 0 000 b9 0 000 b4 0 000 bin 0 000 b5 0 000 Enter the factors value for more information about the equation programming see paragraph 10 2 Press on the F4 key N
52. ext to confirm all the entries and to pass in the following page Multi wavelengths mode 161 Edit method 5 of 6 03 25 08 15 53 Number 2001 Name PROT Version 1 0 Citation form Proteins Unit mg mL Resolution 0 1 All data are displayed once again Press on F1 key Back to correct erroneous entries on the previous pages Press on the F4 key Next to confirm all the entries and to pass in the following page The method is programmed Edit method 6 of 6 03 25 08 15 56 Function 1 150 A 280 nm 0 760 A 60 nm R 1 000 The programmed equation is displayed for more information about the equation programming see paragraph 10 2 Press on F4 key Complete to leave the programming 8 4 Method storing The method is automatically stored in the memory 8 5 Method deletion lt HOME gt Multi wavelengths Select method Setup Edit method Delete Select the method to be deleted with A and W keys and confirm with lt START ENTER gt key 162 Multi wavelengths mode Multi wavelengths 03 25 08 16 05 Zero measurement required 2001 PROT Proteins Method list Transmission Press F1 key Setup It displays Multi wavelengths 03 25 08 16 07 Edit method Measurement data memory Select Edit method with A and W keys and confirm with lt START ENTER gt key Fat method 1 of 6 Number Name Version Citation form
53. ilter Selected values invert selection Delete memory selected values only Delete memory all values The symbol v at the end of AutoStore indicates that the function is active If this function is not active it means that data will be saved manually only with lt STORE gt key Remark The AutoStore setting is only valid for the measuring modes Concentration and Multi wavelengths 11 3 4 Filtering results 11 3 4 1 Filtering a results group lt HOME gt Concentration Absorption Transmission or Multi wavelengths Setup or General setup from main menu Measured value memory Setup Filter It is possible to select a result or a group of results with the Filter function The functions display delete and download stored measurement datasets refer to all stored measurement da tasets that correspond to the specified filter criteria Filter criteria The following filter criteria can be set 204 Memory Mode Absorption transmission and Multi wavelengths User ID identification Date date from to Method for the measured parameters Concentration and Multi wavelength Measurement data memory i 03 18 08 15 31 AutoStore vw Selected values invert selection Delete memory selected values only Delete memory all values Select Filter with A and W and confirm with lt START ENTER gt key The setting filter menu ap pears Multi wavelength Not
54. ilter test Lamp test Wavelength calibration If the test is OK the symbol d js displayed on the corresponding line This self test includes tests of the memory processor interfaces filter and lamp checking of the wavelength calibration After the end of the self test the screen displays the main menu 02 28 08 16 47 Concentration Absorbance Transmission Multi wavelengths LR It is possible to see and print the self test results by pressing F4 key Info see paragraph 15 6 1 select a measuring mode with A and Y keys 2 Confirm with lt START ENTER key 130 Start up 5 2 Warm up time After switching on the photometer requires a warm up time of 15 minutes Reproducibility of measure ment data is restricted during the warm up time Therefore do not measure during the warm up time During the warm up time a progress bar appears on the display next to the date The progress bar disap pears as soon as the warm up time is over Progress bar during warm up time Concentration Absorbance Transmission Multi wavelengths Spectrum Kinetics 5 2 8 Screen backlight The photometer automatically switches off the display illumination if no key has been pressed for 5 minutes The illumination is switched on again with the next keystroke The function of the key becomes active only with the following keystroke 5 2 9 Spectrophotometer switch off To switch the photometer
55. irm with lt START ENTER gt key It displays the curve added with the constant value entered by the operator To come back to a normal screen Press on F2 key Edit Select Original values with A and Y keys Confirm with lt START ENTER gt key 9 7 5 10 Multiplication by a constant lt HOME gt Spectrum Editi Multiply fixed value Multiplies the absorbance or transmission values of the current spectrum by a constant value Press F4 key Open and select the first spectrum or use the acquired spectrum Press F2 key Edit Spectrum Compare spectrum Add spectrum Subtract spectrum Divide spectrum ratio e Add fixed value Select Multiply fixed value with A and W keys Confirm with lt START ENTER gt key Enter the constant value and confirm with lt START ENTER gt key It displays the curve multiplied by the constant value entered by the operator To come back to a normal screen Press on F2 key Cd Select Original values with A and Y keys Confirm with lt START ENTER gt key Kinetics mode 185 10 Kinetics mode 10 1 General The Kinetics function enables the temporal tracing of the absorbance or transmission of a sample at a certain wavelength For recording the spectrophotometer carries out single measurements at regular intervals meas uring interval and stores the measured values as a time function All measuring
56. is not operational for data acquired during this measurement If the operator wants to store data he has to use lt STORE gt key So all results acquired during last measure ment will be stored under the names given by the operator Measurement data me 03 18 08 14 44 03 12 08 10 57 gt 50 00 mgA NO3 Administr 03 12 08 10 56 20 40 mg NO3 Administr Assay01 03 12 08 10 55 12 60 mgANO3 Administr Test02 03 12 08 10 55 12 60 mgANO3 Administr Test Fitter Memory allocati 11 3 3 2 Automatic recording with AutoStore function lt HOME gt Concentration or Multi wavelengths Setup or General setup from main menu Measured value memory Setup AutoStore Press on F1 key Setup It displays Memory 203 Measurement data memory 03 18 08 14 48 AutoStore Filter Selected values invert selection Delete memory selected values only Delete memory all values For the measuring modes Concentration and Multi wavelengths it is possible to save every measured value automatically with AutoStore function All automatically saved measurement datasets are given the label AutoStore If the same measurement da taset is manually saved using lt STORE gt key the AutoStore label is overwritten by ID code given by the operator Select AutoStore with A and W keys and confirm with lt START ENTER key Measurement data memory 03 18 08 14 57 AutoStore F
57. it Divide spectrum ratio Divide the absorbance or transmission values of the current spectrum by the values of a stored spectrum Warning This function always applies to the common wavelength range of both spectra only Press F4 key Open and select the first spectrum or use the acquired spectrum Press F2 key Edit im 2k Edit t Surface calculation Derivative Compare spectrum Add spectrum Subtract spectrum Select Divide spectrum ratio with A and Y keys Confirm with lt START ENTER gt key Select the second spectrum and confirm with lt START ENTER gt key It displays the ratio of the two spectra To come back to a normal screen first selected spectrum Press on F2 key Cd Select Original values with A and Y keys Confirm with lt START ENTER gt key 9 7 5 10 Addition of a constant lt HOME gt Spectrum Edit Add fixed value Add a constant absorbance or transmission value to the current spectrum Press F4 key Open and select the first spectrum or use the acquired spectrum Press F2 key Edit 184 Spectrum mode ol d NEO Edit Derivative Compare spectrum Add spectrum Subtract spectrum F Divide spectrum ratio Add fixed value Select Add fixed value with A and W keys Confirm with lt START ENTER gt key Enter the positive or negative fixed value and conf
58. l If the operator does not want that the samples absorbance is displayed at the same time as the concen tration he has to select it in the Setup menu Press on F1 key Setup It displays Dilution Sample blank value Display absorbance New method Edit method Delete method Measurement data memory Select Display absorbance with A and W keys and confirm with lt START ENTER key Concentration 03 20 08 15 00 TAD i 1001 Nitrate NOS 0 00 30 00 mg l Proceed as the same way to display again the samples absorbance NOTE The symbol d at the end of Display absorbance means that the absorbance will be displayed at the same time as their concentration 148 Concentration mode 6 4 1 7 Printing results Press lt PRINT gt key to send data on printer UviLine 6100 7449001 1 16 Willy Time of measurement 3 20 2008 16 28 23 Method Nitrate Measured value gt 30 00 mg l Sample absorbance 1 119 Time of measurement 3 20 2008 16 28 23 Method Nitrate Measured value 17 50 mg l Sample absorbance 0 604 6 4 1 8 Sample dilution It is possible to program a dilution factor From O to 999 that will be taken in account during sample measurement Before sample measurement when the screen displays TAD w Absorbance 0 272 1001 Nitrate NOS 0 00 30 00 mg l Press F1 key Setup It displays Concentration Dilution Sample blank value Display abso
59. lamp Don t forget to set this counter to zero after changing the halogen lamp of UviLine 9100 See previous para graph The lamp of UviLine 9400 usually does not need to be changed 13 6 Device information Following information relating to the soectrophotometers are displayed Instrument designation Instrument serial number Number of the instrument software version Updating date of the software Hardware version Hardware status for servicing Number of lamp working hour Results of the tests of the most important parts of the spectrophotometer Memory status Registered user lt HOME gt Infol 03 03 08 16 36 Model designation UVILINE 9100 Serial number 07010001 Software version Build Hardware version Hardware status FF 00000000 Lamp counter 0 System test vg Filter test vg Lamp test ra Wavelength calibration vg Free internal memory space 26098900 KB Registered user The information about the instrument and the autotest results are displayed and can be printed Press lt ESC gt key to come back to the main menu Various adjustments 219 13 7 Timer This Timer menu allows storing in memory a waiting time before to start a measurement Ex Incubation time of a reactional mixture This Timer acts like a stop watch 03 03 08 16 46 Designation Status User defined ti 00 15 00 Inactive Press F3 key Edit to enter a delay Press F1 key Start to start th
60. leted Spectrum mode 179 9 7 5 4 Area calculation lt HOME gt Spectrum Edit Surface calculation Calculate the surface under the curve between two wavelengths X1 X2 freely chosen by the operators Press on F2 key Cd semen TT ee Ai lt amp valleys detection Mark points A Suitace calculation Derivative Compare spectrum Add spectrum Select Surface calculation with A and W keys Confirm with lt START ENTER gt key s L 14 Press on F3 key X1 to select left cursor Move with lt and gt keys the left cursor along the curve until the first wavelength Press on F4 key X2 to select right cursor Move with lt and gt keys the right cursor along the curve until the second wavelength The surface value and the selected wavelength range are displayed at the top right corner of the screen 180 Spectrum mode 9 7 5 5 Derivative calculation lt HOME gt Spectrum Editl Derivative Calculate the derivative of the complete spectrum For the calculation of the second and the third derivative it is possible to carry out the function on several times Press on F2 key Cd 0 8 d i Edit Peaks amp valleys detection Mark points 4 Surface calculation Derivative Compare spectrum Add spectrum As 444 0 Abs 0 215 Original curve Press on F2 key Edit Select Original values with A
61. letes the character left of the cursor during character entries Moves the cursor to the left in a spectrum or kinetic diagram Moves the cursor to the right in a spectrum or kinetic diagram functions are displayed in the function key menu at the bottom edge of the display Overview 123 4 2 3 Use of alphanumeric keyboard Numerals letters punctuation marks and special characters are entered with the alohanumeric keypad of the meter or using an external keyboard 4 2 3 1 Zeichensatz The following characters are available e Numerals 0 9 e Letters A Z and a Z e Punctuation marks and e Special characters A 2 u et 4 2 3 2 Command principle Entering characters is always possible if there is an input field on the display 2 The numerals and characters expect for the small letters assigned to the keys of the alphanumeric key pad are printed on the keys Example With the lt 2 ABC gt key you can enter the following characters 2 A B C a b c Select the required character by pressing the key several times similar to a mobile phone When press ing a key that is assigned to several characters once the respective numeral appears first To enter a numeral one key pressing is always sufficient When pressing the key for the first time a line pops up that displays all characters possible with this key The currently selected character is highlighted A character is
62. me is opened 128 Start up 1 With A and Y keys select one option from the menu Confirm this option with lt START ENTER gt key The input field for the current date pops up 28 2008 2 Enter the current date with lt 0 9 gt keys and confirm The input field closes The date is accepted 3 Select Time and confirm The input field for the current hour pops up 2 28 2008 4 Enter the current hour with lt 0 9 gt keys and confirm The input field closes The time is accepted NOTE After the initial commissioning the operator can change the date and time at any time From the main menu lt HOME gt enter in the menu General setup then Date time menu During the initial commissioning the instrument automatically guides the operator to the self test after the setting of the date and time 5 2 6 Self test IMPORTANT During self test the cuvette compartment must be empty without any cuvette inside the cuvette well and the cover of the cuvette compartment must be closed Self test ll 02 28 08 16 38 Please make sure no cell is inserted and the cover is closed Then press lt START ENTER gt 1 Be sure that the cuvette compartment is empty Start up 129 2 Press lt START ENTER gt key to run the self test Self test l 04 11 08 10 40 Keep cover closed System test Filter test Lamp test Wavelength calibration Self test Keep cover closed System test F
63. ments interval Max interval setting the interval is defined differently see below Delay Time between the start of the recording and the start of the first single measurement This time allows avoiding the no linear part of a reaction Format hh mm ss hours minutes seconds Scaling Auto or Manual Scaling Auto The spectrophotometer adjusts the scaling of the axes minimum and maximum value of the axis to the measured values while measuring The entire curve is always visible Scaling Manual Abs min Abs max The scaling of the axes minimum and maximum value of the axis is permanently set manually Press on F4 key Next It displays Edit method 2 of 2 04 09 08 15 58 Measurements interval L interval Katalytic activity Measurements interval 1 interval or Max interval Here it is possible to define how many measurements will be carried out per interval The setting affects the calculation of the slope of the individual intervals The displayed slope for an interval is determined as follows depending on the profile Measurements interva 1 interval Max interval Slopes Difference of two measured values 2 points calculation Slope of the straight line determined in an interval by linear regression Katalytic activity YES or NO Result Total slope report to the minute Total slope report to the minute multiplied by a factor Katalytic activity NO YES
64. mum 361 1 nm 418 6 nm 446 5 nm 460 7 nm 936 8 nm 638 0 nm Minimum 353 1 nm 406 5 nm 432 3 nm 457 0 nm 916 9 nm 613 9 nm It detects automatically the peaks maximum and the valleys minimum 176 Spectrum mode Manual input Minimum distance between min max wall Enter the minimum distance between minimum and maximum REMARK More the difference between minimum and maximum will be small more peaks and valleys will be listed Press F1 key OK to come back to the curve Press F4 key Mark to mark on the curve minimum and maximum See paragraph 11 7 5 2 Press lt PRINT gt to print curve with minimum and maximum UVILINE 9100 7449001 Administrator Administrator 3 27 2008 16 14 48 Holmium csv Absorbance ae beeen newer eg eeeeee iassass DEE STEET DEET DEED EETTTETTTTETETTTAAbebh ehebhdelkk D I D I s ssssrs sss iss DER DI s s s Absorbance o See Dee 800 1000 Wavelength nm Maximum 361 7 nm 0 348 419 1 nm 0 158 447 0nm 0 882 461 3nm 0 478 537 3nm 0 195 638 5 nm 0 116 Minimum 353 3nm 0 134 406 5 nm 0 095 432 8nm 0 094 457 6nm 0 362 519 4nm 0 070 616 0nm 0 055 9 7 5 2 Punkte markieren lt HOME gt Spectrum Edit Mark points This function makes it possible to the operator to mark on the spectrum a particular point with its coordinates Absorbance Wavelength Spectrum mode 177 Press on F2 key Cd 03 26 08 16 30 A
65. n Filter x Administrator Memory allocation 23 1000 Number of results in memo Single value Citation form If there are more datasets available than can be displayed the arrows A and W displayed additionally 27 03 07 14 00 3 50 mg l Ni Administrator AutoStore Filter indicates that filter settings are active In this case only those datasets are displayed that correspond to the selected filter criteria see paragraph 11 3 4 11 3 2 Transferring results 11 3 2 1 Adjustment for results transmission 11 3 2 1 1 Decimal separator for csv Files lt HOME gt General setup Data transfer Printer Decimal separator for csv Files For the output of CSV files it is possible to select a comma or point as the decimal separator Select Point 12 34 or Comma 12 34 11 3 2 1 2 Data format lt HOME gt Configuration Data transfer Printer Data format print When printing measurement datasets it is possible to select a short or long version with different information contents Select Short or Extended 11 3 2 1 3 Baud rate for RS232 interface lt HOME gt Configuration Data transfer Printer Baudrate for printer For printers run at the RS232 interface the baud rate can be set Select 1200 2400 4800 9600 or 19200 11 3 2 2 Transferring data to printer Data printing is available with the following printers Dot matrix printer connected on the RS232 interface
66. nce vd New method Edit method Delete method Measurement data memory Select Delete method with A and W keys and press lt START ENTER gt key 6 4 Method application 6 4 1 With standard solutions Standard ID Standard manufacturer Blank value Standard conc 1 Standard conc 2 Standard conc 3 30 0 mg l 138 Concentration mode 6 4 1 1 Measurement When standard s are entered press F4 key Next It displays Concentration 03 19 08 15 42 Blank value BEE Std 1 10 00 mg l Std 2 20 00 mg l Std 3 30 00 mg l Measurement Press F3 key Measurement to start the measure Blank value 03 19 08 15 44 Zero measurement required Nitrate Press on lt ZERO BLANC key to perform the zero of the meter Blank value 03 19 08 15 46 Start measurement with lt START ENTER gt Install the blank if necessary inside the cuvette holder Start the blank measurement if this one was programmed by pressing lt START ENTER gt key Concentration mode 139 Blank value 03 19 08 15 51 Last measured absorbance 0 053 Median 0 053 1 Measurement s Nitrate It is possible in the event of error to refuse the value and to measure again the solution Press then on F2 key Discard Then remake measurement while pressing on lt START ENTERS key It is also possible if the solution is unstable to measure it again several times The software will make the
67. nfirm the security prompt The user account is deleted 13 Various adjustments 13 1 Language selection lt HOME gt General setup Language English 1 With A and VW keys select the language 2 Confirm the selected language with lt START ENTER gt key The language is selected The language actually selected is noted with the symbol d 216 Various adjustments 13 2 Setting date and time lt HOME gt General setup Date Time Date 3 3 2008 15 21 49 The menu Date Time is opened 1 With A and VW keys select an option of the menu Confirm this option with lt START ENTER gt key The input field which allows entering the current date opens up Date Time 03 03 08 15 27 Date 3 2008 2 Enter the current date with lt 0 9 gt keys and confirm The input field closes The date is accepted 3 Select Hour and confirm The input field which allows entering the current hours opens up Date Time 03 03 08 15 33 3 3 2008 4 Enter the current hour with lt 0 9 gt keys and confirm The input field closes The time is accepted Various adjustments 217 13 3 Adjusting contrast lt HOME gt General setup Display settings Here it is possible to adapt the contrast to the lighting conditions Display settings 03 03 08 15 41 1 Select and confirm Contrast An adjustment system using a cursor is displayed to adjust the screen con
68. o tometer To change it proceed as follows i CB en 1 Fig 8 Halogen lamp protection cover 1 Switch off the spectrophotometer and disconnect it from the main power 2 Remove the two screws 1 and remove the lamp protection cover 2 WARNING The lamp becomes very hot during operation Do not touch the hot lamp because it can cause burns The lamp should cool down for approx 10 minutes before it is exchanged 222 Maintenance Fig 9 Halogen lamp changing 3 Disconnect the electrical plug connection 3 Disconnecting it might take a lot of effort 4 Unscrew the two knurled head screws 4 and remove the defective lamp module 5 5 Fix the new lamp module with the knurled head screws When doing so the metal plated side of the PCB must point outward toward the knurled head screws 6 Reconnect the power supply cable 7 Reinstall the protection lamp cover 8 Reset the service hour counter for the lamp module see paragraph 13 5 Maintenance 223 14 2 Changing buffer batteries Information Only use leak proof alkaline manganese batteries If you leave the spectrophotometer switched on during the exchange or insert the new batteries within a minute after taking out the old ones the date and time are re tained in the spectrophotometer Fig 10 Changing buffer batteries 1 Turn on the spectrophotometer upside down and place it on a soft surface 2 Remove the cover 1 of batteries compartment 3
69. o MMO eA E 13 8 Software update 14 Mantenance ek ENNEN ENEE ENN ENNEN NENNEN ENNEN ENN ENNEN 14 1 Changing lompe 14 2 Changing buffer batteries 0 n00nnannonnnnnoennnnnnnnnnnnnnennnnnnne 14 3 OUeanemg TAA e Ee De E is E 14 5 Troubleshooting n nnaneennonnnennnnnennennnnnsnronrsnennernrrnrenrenrenne 15 Connecting accessories cccceeeeeeeeeeeeeeeeseneeeeneeees 15 1 Communication Interface Declaration of conformity SI Analytics last page of document Version 130425 US Remarks 117 1 Remarks The information contained in this document may be the object of patents or patent applications by SI Analytics The possession of this document does not confer any license to such patents The following names are SI Analytics registered trademarks throughout the world UviLine 9100 and UviLine 9400 This manual is updated periodically The updates are included in the new editions All information supplied in this edition of the manual may be amended before the products described herein are available All reproduction rights reserved 1 1 Information The SI Analytics equipment has been designed manufactured tested and inspected according to the ISO 9001 2008 standards If the unit is not immediately installed it should be stored in a dry and clean area The storage tempera ture should be between 10 and 35 C SI Analytics equipment is carefully inspected before it is packed As soon as you
70. off keep the lt ON OFF gt key depressed until the photometer is switched off Concentration mode 131 6 Concentration mode 6 1 Method programming lt HOME gt Concentration New method or Last used If necessary come back to the main menu with lt HOMEs gt key Select with A and W keys the Concentration mode Enter in the concentration mode Concentration Absorbance Transmission Multi wavelengths Confirm with lt START ENTERs gt key If there is no method in memory the following screen will appear Select method all 03 19 08 13 21 132 Concentration mode Press F1 key New method It displays Number Wavelength Resolution Unit Citation form Designation Version Calibration curve Measure standard solutions The following parameters can be modfied Number Automatic classification of methods From 1001 to 1100 Wavelength 320 1100 nm for UviLine 9100 190 1100 nm for UviLine 9400 Resolution From 0 to 3 It is the number of figures after comma for the result Unit i e mg L 10 characters maximum Citation form 18 characters maximum Enter the chemical formula of the compound to be measured i e NO3 Designation 18 characters maximum Enter the compound name i e Nitrate Version 18 characters maximum Enter the parameter concentration range i e 0 9 75 3 mg L Number 1001 Wavelength 500 nm Res
71. olution 0 1 Unit mg l Citation form NOS Designation Nitrate Version 0 9 75 3 mg L Calibration curve Measure standard solutions Concentration mode 133 Calibration curve Measure standard solutions Enter formula Enter couples of values Calibration curve There are several possibilities To measure the standard solutions To enter the equation of the calibration curve or the factor value To simulate measurements while entering of the couples of Absorbance Concentration values Select an option and press F4 key Next MEASUREMENT OF STANDARD SOLUTIONS Standard ID Standard manufacturer Blank value Standard conc 1 Standard ID Enter the identification number of the standards if this one exists Standard manufacturer Enter the standard manufacturer name if this one exists Blank value YES or NO Measure d or not of the reagent blank Measurement example without reagent blank blue curve and with reagent blank purple curve It is easy to notice that for a same sample which absorbance measured against the zero is 0 200 Abs the concentration value will not be the same The blank value is subtracted from the measure 134 Standard conc 1 Concentration mode y 0 01x 0 0002 R 4 y 00093x 0 0606 F 0 9929 With blanc Without blank Concentration Enter the concentration of standard 1 Remark It is possible
72. orized method Setup Measured value memory From Absorption Transmission menu lt HOME gt Absorption Transmission Setup Measured value memory From Multi wavelengths menu lt HOME gt Multi wavelengths Choice of a memorized method Setup Measured value memory Each one of these possibilities displays the contents of the measurement data memory in the form of list in the following way Concentration 03 12 08 10 48 Dilution Sample blank value Display absorbance New method Edit method Delete method Measurement data memory Select Measurement data memory with A and W keys and confirm with lt START ENTERs gt key Memory 201 Measurement data me 03 12 08 11 09 03 12 08 10 57 gt 50 00 mgA NO3 Administr 03 12 08 10 57 gt 50 00 mgA NO3 Administr AutoStore 03 12 08 10 56 20 40 mgA MO Administr Assay01 Manual memory setting 03 12 08 10 56 20 40 mgA MO Administr AutoStore with ID Measurement date and time 03 12 08 10 55 12 60 mgA NO3 Administr Test02 03 12 08 10 55 12 60 mgA NO3 Administr Test01 03 12 08 10 55 12 60 mgA NO3 Administ AutoStore Automatic memory setting 03 12 08 9 50 9 00 mgANO3 Administr AutoStore 03 12 08 249 9 00 mgA NOS Administr AutoStore 03 12 08 9 49 9 00 mgANO3 Administr AutoStore Besut ofthe measurement 03 13 08 9 49 9 00mq NO3 Administr AutoStore Indication of hidden results Fitter selectio
73. ower pack to an easily accessible power socket The display illumination switches on and then off again 5 2 3 First photometer activation During the initial commissioning the spectrophotometer automatically guides the user through the setting of the meter language date and time after switching on see following sections Press key The spectrophotometer is switched on It displays Language Deutsch English Francais Espa ol Italiano The screen switches on the language setting see paragraph 5 2 4 After language selection the spectrophotometer carries out the self test Start up 127 5 2 4 Language set up During the first start up the operator is guided directly to the language setting Langue Language i 04 02 08 14 57 Francais 1 With A and W keys select the language 2 Confirm the choice with lt START ENTER gt key The language is selected The actually selected language is marked with this symbol i The screen switches on the adjustment of the parameters Date and Time see paragraph 5 2 5 NOTE After the initial commissioning the operator can change the language at any time From the main menu lt HOMEs enter in the menu General setup then Language menu 5 2 5 Date and time set up During the initial commissioning the instrument automatically guides the operator to the setting of the time and date after the setting of the language The menu Date Ti
74. r Selection of one method Select a method with A and VW keys The active selection is displayed in reverse video Press on lt START ENTER3 gt key to confirm the method choice The spectrophotometer is operational 190 Kinetics mode 10 5 1 Limitation of methods list If the list is very long it is possible to narrow down the methods list and thus make the search easier as fol lows Press on F4 key Last used it is possible to restrict the method list to the ten methods last used With the search function it is possible to search certain character strings in the list The search takes place as a full text search of the entire list contents Thus the operator can search for a method number or name 10 5 2 Research function Search for a character string Enter the character string to be searched for in the search window with lt A 9 gt The list appearing below shows all methods containing the character string This methods list is updated with each character that is entered Select method all 04 11 08 10 54 Absorbance Mev Method oe Remark Note the case sensitivity when searching 10 6 Method application lt HOME gt Kinetics Select method all 04 10 08 9 11 4001 NADH Absorbance 4002 TESTO1L Absorbance Select a method with A and W keys and Press on lt START ENTER gt key to confirm the method choice It displayed Kinetics mode GEES Zero mea
75. rbance vd New method Edit method Delete method Measurement data memory Press on lt START ENTERS key Sample distilled water Concentration mode 149 Enter the dilution factor 1 0 no dilution 1 1 dilution by 2 When the dilution factor is entered confirm with lt START ENTER gt key It displays Concentration i 03 20 08 15 21 Start measurement with lt START ENTER gt 20 00 60 00 moll The dilution factor i e 1 1 is displayed on the top left part of the screen NOTE This dilution factor will be maintained with the value entered by the operator for all measurements to come At the time of the exit of the mode of analysis or analysis in progress it will be given to O no di lution 6 4 1 9 Sample blank value It is possible to subtract a sample blank for each measured sample Press F1 key Setup after zero measurement It displays Sample blank value Display absorbance New method Edit method Delete method Measurement data memory Remark The symbol d at the end of Sample blank value means that the option is selected Select Sample blank value with A and W keys and confirm with lt START ENTER key 150 Concentration mode Concentration 03 25 08 13 30 Sample blank value Start measurement with lt START ENTER gt The symbol SB appears at top left corner of the screen and stays here and will remain all the time that this op
76. receive your equipment check the condition of the packaging and if you notice any problems notify your carrier within 48 hours Then consult the packing list and check that everything is in order Finally if you discover that something is missing or if the goods are damaged immediately notify SI Analytics SI ANALYTICS Hattenbergstr 10 D 55122 Mainz Germany Allemagne Alemania Tel 49 0 6131 66 5111 Fax 49 0 6131 66 5001 E mail si analytics xyleminc com Guarantee The new equipment and material sold by SI ANALYTICS is guaranteed against any manufacturing de fects for 2 years unless otherwise stated by SI Analytics The SI ANALYTICS company guarantee applies exclusively to defectiveness arising from a design fault or from a concealed defect It is strictly limited to the free dispatching of replacement parts except for consumable items or to the repairing of the equipment in our workshops within a deadline of 10 working days shipping delay not included By express agreement the following are strictly excluded from our guarantee All damages notably for staff costs loss of earnings business trouble etc Any breakdown due to an incorrect use of the equipment non adapted mains fall attempt at trans formation etc or to a lack of maintenance by the user or to poor storage conditions Any breakdown due to the use of parts not supplied by SI ANALYTICS on SI ANALYTICS equipment Any breakdown due to th
77. remove out of the compartment the old batteries 4 Insert the four new batteries in the battery compartment Make sure that the poles of the batteries are in the correct position The signs on the batteries must correspond to the signs in the battery compartment 5 Close the batteries compartment cover Batteries life time The power consumption of the clock is very low The lifetime of high quality batteries is at least 5 years 14 3 Cleansing WARNING The housing components are made out of synthetic materials ABS PMMA and PC Thus avoid contact with acetone ethyl alcohol and similar detergents that contain sol vents Any splashes must be wiped off immediately Clean your photometer as follows e If the housing surface is dirty wipe it with a soft cloth and mild soapy water e Remove any chemicals splashes as soon as possible e For disinfection you can use isopropanol for cleaning for a short time 224 Maintenance 14 4 What to do if 14 4 1 Measures if cell is broken WARNING Cells can contain dangerous substances If the contents are released follow the safety instructions in the package insert If necessary take corresponding protective measures protective goggles protective gloves etc CAUTION Do not turn the photometer upside down to remove the liquid When doing so the liq uid could come into contact with electronic components and damage the photometer The photometer has a drain device thro
78. rk of Xylem Inc or one of its subsidiaries 2013 Xylem Inc Version 130425 US EDV 8252588
79. rs Each user is a member of a user group with defined user rights 12 1 User groups There are three hierarchical user groups Administrator top level User user account registered by the administrator Guest user without user account Administrators and users log in to the photometer with their user name and password Guests can optionally enter a name for their login Thus documented measured values can later be assigned to the user 12 2 Details of user rights aon itt er Gu Legend NEE AE E AE A bg A0 E AE A E E E A EC AE A a N sk Se S E EE EE dE lt vd Peteananodstredinmney JL LO SO eS Doozepmegne LOI Jemen LOS imine SET e emp vc L l v SPR TAS 212 User management 12 3 Activating deactivating user management Each user can activate the user management function If the function is deactivated each user has adminis trator rights Only members of the user group administrator can deactivate the user management function If the function is active each user has to log in the spectrophotometer After the login the user has certain rights depending on the user group 12 3 1 Activating user management lt HOME gt General setup User management User management not active Activate user management Select YES and confirm The user management function is active 12 3 2 Deactivating user management lt HOME gt General setup User management
80. s f E weasurement data Cancel Filter s Memory alocation 29 1000 Single value Memory Select OK or Cancel 11 3 5 2 Memory deletion lt HOME gt Concentration Absorption transmission or Multi longueurs d onde Setup or General setup from main menu Measured value memory Setup Delete memory all values It will delete all memorized results 207 Select Delete memory all values with A and W keys and confirm with lt START ENTERs gt key It will display Measurement data memory 03 12 08 10 57 gt 50 00 mg NO3 Ad 03 12 08 9 49 9 00 mg l NO3 03 12 08 9 49 9 00 mg l NO3 03 12 08 9 49 9 00 mg l NO3 Fitter vi Memory allocation 29 1000 Select OK or Cancel 11 3 5 3 Deletion of results lt HOME gt Concentration Absorption transmission or Multi wavelengths Setup or General setup from main menu Measured value memory Delete It is possible to delete only one result Proceed as follows Measurement data me 03 12 08 10 57 gt 50 00 ma NO3 Administ 03 12 08 10 57 gt 50 00 mg NO03 Administ Administr inst Test02 Pest ww AutoStore vo AutoStore aa AutoStore aa AUtoStore AutoStore 03 12 08 10 56 20 40 mg l MO 03 12 08 10 56 20 40 mg NO3 03 12 08 10 55 12 60 mg l NO3 03 12 08 10 55 12 60 ma l NO3 03 12 08 10 55 12 60 mol NO3 03 12 08 9 50 9 00 mg l NO3 03 12 08 9
81. surement required Absorbance Press lt ZERO BLANK gt key When zero is done it displays Start measurement with lt START ENTER gt 4001 NADH Insert sample Press lt START ENTERS key to start the measurement Kinetics 04 10 08 9 22 4001 MADH Duration 00 00 20 The spectrophotometer starts recording automatically Wait the end of measurement Stopping the recording 191 Press on F4 key Stop to terminate the recording prematurely The curve recorded up to this point can be stored and edited see paragraph 12 8 3 Press on lt ESC gt key to completely cancel measurement The curve recorded up to this point is discarded After the specified Duration has expired the cursor appears 192 Kinetics mode 4001 NADH Number of measurements 3 Duration 00 07 00 Interval 00 00 30 Press on lt START ENTERS key to run a new measurement 10 7 Method editing lt HOME gt Kinetics Select method Setup Edit method Enter in Kinetics mode Select a method with A and W keys confirm with lt START ENTER gt key Zero measurement required 4001 NADH Absorbance Method WE Press F1 key Setup It displays Edit method Select Edit method with A and W keys confirm with lt START ENTERs gt key Kinetics mode 193 10 8 Kinetics treatment 10 8 1 Printing kinetic curve Press lt PRINT gt key to outpu
82. t the kinetic curve to a connected printer as a graphic 10 8 2 Cursor The cursor consists of a horizontal and vertical line that crosses each other on a point of the curve A box names the x and y values of the point of the curve Move the cursor along the x axis time axis with lt and gt keys By this way it is possible to trace and evaluate the curve point by point These points are corresponding to the measurement interval 4001 NADH Number of measurements 3 Interval 00 00 30 10 8 3 Saving kinetic curve Press lt STORE gt key to save the kinetic curve As the memory location it is possible to select the spectroophotometer memory Internal DataB folder or a connected USB memory at the USB A connection USB memory If the operator forgot to save the curve this one is nevertheless kept in memory under the name of date kinet icsBackup csv 10 8 4 Loading a stored kinetic curve lt HOME gt Kinetics Open Press F4 key Open Open Internal DataB folder 04 10 08 10 09 04 10 08 Kinetics 4001_080410_1005 csv 04 10 08 NADH csv 04 10 08 KineticsBackup csy The list with the stored kinetic records Internal DataB folder is displayed Press F1 key Location to select another memory location of the kinetic record USB memory for a USB medium at the USB A connection Select the kinetic curve and confirm The original view of the curve is displayed 194 Kinetics mode 10 8
83. taken over in the input field if e the character is highlighted for more than one second e the character is confirmed with lt START ENTER3 gt e another alphanumeric key is pressed Remark During mere number entries Such as entering a wavelength the keys of the alphanumeric keypad are assigned to the respective numeral only Each pressing key directly enters the numeral like a pocket cal culator 4 2 3 3 Special characters To enter special characters use lt 1 gt key 4 2 3 4 Correction of bad character Use lt lt gt key to delete all characters until THE INCORRECT one and start the entries from there again Overview 124 4 3 Display Absorbance Transmission Fig 4 Screen 1 Status line actual status date and time 2 Displaying area for the menus or measurement results 3 Menu for function keys 4 4 Cell chamber Fig 5 Cuvettes well The beam goes from left to right Start up 125 5 Start up 5 1 Packaging The UviLine 9100 and UviLine 9400 spectrophotometers are supplied with gt Power pack connection cable incl adapters international use gt Buffer batteries 4 x AA manganese alkaline gt 6 diaphragms 0 9 x 11mm 1 8 x 11mm 3 8 x 11mm 1 8 x 2 3mm 3 8 x 2 3mm 1 8 x 4 8mm for use with special micro cuvets gt Users manual operating instructions Packing This photometer is sent out in a protective transport packing We recommend Keep the pack ing material
84. tion is selected or that the operator did not leave the analysis in progress or the mode of concen tration measurement Insert the first sample blank and run the measurement by pressing lt START ENTER gt key Sample blank value 03 25 08 13 38 Last measured absorbance 0 034 Median 0 034 1 Measurement s Discard As for the blank the operator can reject measurement F2 key Discard or make several times the measurement of the same sample blank F1 Key Next meas and preserve the average value Press F4 key Apply to run the sample measurement It displays Concentration 03 25 08 13 45 Start measurement with lt START ENTER 0 00 1000 00 mg l Method list Insert the sample corresponding to the measured sample blank and start the measurement by pressing lt START ENTERS key It displays Concentration mode 151 1 19 Absorbance 0 630 0 00 1000 00 mg l It displays the sample absorbance read against the zero i e 0 630 and the sample concentration calcu lated as following Sample concentration sample absorbance read against the zero minus sample blank absorbance read against the zero multiply by the factor or reported to the calibration curve 6 4 2 With value couples Concentration 03 20 08 15 278 Standard ID Standard manufacturer Blank value TTT Standard conc 1 10 00 mg l Standard conc 2 20 00 mg l Standard conc 3 30 00 mg l _ nid Press on F4 key Next
85. to the limits but absorb ances will be displayed see example under lt 10 00 Absorbance 0 437 10 00 30 00 mg l 144 Concentration mode 6 4 1 3 Point deletion To delete a point press F1 key Back It will display once again Concentration 03 20 08 14 01 Number 1002 Wavelength 500 nm Resolution 0 01 Unit mg l Citation form NOS Designation Nitrate Version 0 9 75 3 mg L Calibration curve Measure standard solutions Press F4 key Next It displays Concentration 03 20 08 14 03 Standard ID Standard manufacturer Blank value Standard conc 1 10 00 mg l Standard conc 2 20 00 mg l Standard conc 3 30 00 mg l ndi Select the point to delete with A and W keys Concentration 03 20 08 14 05 Standard ID Standard manufacturer Blank value Standard conc 1 Standard conc 2 Standard conc 3 Delete the point by pressing F3 key Delete Concentration 03 20 08 14 06 Standard ID Standard manufacturer Blank value Standard conc 1 Standard conc 2 Concentration mode Press on F4 key Next Concentration 03 20 08 14 27 Blank value 0 053 Std 1 10 00 mg l 0 350 Std 3 30 00 mg l 1 012 During this stage it is always possible to measure a standard once again Select the standard to measure again Press F3 key Measurement to start the standard measurement Press F4 key Nex to display the curve 03 20 08 14 32 fix OD D r 0 0432
86. trast 2 Adjust the screen contrast by pressing lt and gt keys and confirm with lt START ENTER gt key 3 Come back to the main menu with lt ESC gt key 13 4 Reset It is possible to reset initialize the measurement settings or all settings Remark The reset function is only available for users belonging to the user group Administrator There are different possibilities to reset the instruments settings Parameters reset All settings are deleted except the memory which contains measurements results methods defined by the user and the measured blank values Delivery conditions All settings including measurement data memory and user defined methods are deleted and the spectrophotometer is reset to the de livery condition Service lamp counter Allow to reset to zero the service lamp counter after the lamp changing lt HOME gt General setup Reset The menu where to select the reset type Delivery condition Reset configuration is displayed 1 Select and confirm the reset type The reset is carried out 218 Various adjustments 13 5 Lamp servicing count down The photometer counts the operating hours of the lamp The information on the operating hours of the lamp is given in the nfo menu For UviLine 9100 the number quoted there corresponds to the number of operating hours of the halogen lamp For UviLine 9400 this number corresponds to the number of flashes of the xenon
87. ugh which the contents of a broken cell can drain off without causing any damage Proceeding after a cuvette is broken 1 Switch off the spectrophotometer and disconnect it from the main power supply 2 Let the liquid drain off into a suitable container and dispose of it properly according to the instructions of the reagent package 3 Carefully clean the cuvette well using a wet lint free cloth 4 Let dry the cuvette well Remark After recommissioning check out the spectrophotometer for all measurements Maintenance 225 14 5 Troubleshooting The spectrophotometer doesn t switch on Cause Remedy The power cable is not connected Connect correctly the power cable Acoustic signal when operator presses the key Cause Remedy The key has not function in actual part of Press another key the software Measurement range undercut or exceeded Cause Remedy Method not suitable Select another method which has an ap propriate measuring range Press another key Not stable results Cause Remedy The cover of cuvette compartment is Close the cover of the cuvette compart opened ment The halogen lamp arrives at its end life Change the halogen lamp Erroneous measured values Cause Remedy Cuvette dirty Clean the cuvette Wrong dilution Make a dilution again The selected method is not appropriated Select another method Erroneous zero measurement
88. velength mmm a2 Abs 250mm a2 we w2 o wenn a Jelo e o weenn m Jalo nn Wavelengths as fw o f B In Wavelengine a Cf a o o Wavelength aj a o f w In Wavelengins wl fw o w o Wavelengno as fa o w o Wavelengin10 Ato fat o wo o Allen correction It allows measuring the pick high inside a first order chemical noise Equation 4 Abs Apso fl Abs3 _ 2x Abs2 Absl Abs3 2 2 To convert the general equation 1 in specific equation 4 the operator has to make the following program ming wm ri mom a vae o me ro w e Wee om At soan a 1 nn wavelenstn2 mmm a2 mem a2 2 w 0 Wavelengtho zaznm Ad ep a9 a bo o Wegen m a wavelnsth5 AS Wegen ze Wee e Wegen m waens w RE Wavelength 10 Multi wavelengths mode 159 8 3 Method programming lt HOME gt Multi wavelengths Setup New Method If necessary come back to the main menu with lt HOMEs gt key Select with A and Y keys Multi wavelengths mode Enter in the multi wavelengths mode 03 25 08 15 16 Absorbance Transmission Multi wavelengths Spectrum Confirm with lt START ENTERs gt key It displays Select method all 03 25 08 15 25 New Method Press F1 key New method It displays Number 2001 Name PROT Version 1 0 Citation form Proteins Unit Resolution M

UviLine 9100-9400

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