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NISSUI Product Catalogue (日水產品目錄）

1. Dulbecco s PBS Nissui 05913 100 g 08190 Powder 9 6 g x 10 Packs 08192 96 g x 1 Pack 2 Years Directions Formula Dissolve 9 6 g of the medium in distilled water and adjust the volume to 1 000 mL It can be sterilized by autoclaving 121 C Sodium Chloride 8 000 0 mg 15 minutes or by membrane filtration Potassium Chloride 200 0 mg Close tightly and store at 2 5 C in a dark Monopotassium 200 0 and dry place May be stored up to two 2 Phosphate eae year Precautions Back to the Index Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose 19 5 C NISSUI PHARMACEUTICAL CO LTD Glutamine Nissui 2 Years 05908 Powder 0 3 Directions Add 10 mL of sterile distilled water and dissolve completely This solution is used as a supplement to L glutamine free autoclavable media for example Eagle s MEM Nissui 1 Storage and Expiration Store at 2 5 C in a dark and dry place May be stored up to two 2 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life
2. 25 tests RT solution and 40 Potassium Hydroxide Test solution VP into certain wells After addition of specific reagents compare the color developed with the attached color chart and determine as positive or negative results Simultaneously an oxidase test shall be performed and record in the result form 1 year 100 tests Interpretations Refer ID Test EB 20 ANALYTICAL PROFILE for identification Formula Kit component 06626 ID Test EB Plate 25 plates x 20 wells Sterilized Liquid Paraffin 20 mL x 1 vial Result Form 13 sheets x 2 sets ID Test EB Broth 2 5 mL x 25 tubes Color Chart 1 sheet Kit component 06628 10 Ferric Chloride Test 6 mL x 1 vial solution Kovacs Reagent 6 mL x 1 vial 6 a naphthol Test solution 6 mL x 1 vial 40 Potassium Hydroxide mlx 4 vial Test solution Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD ID Test e EB 9 Nissui Identification Test Kit for intestinal bacilli 6 g o VAN f gt z lele elele lt gt e e e 9 L F 06673 06671 Features of ID Test EB 9 Identification of bacteria is very complicated and there are so many biochemical test need to be done ID Test EB 9 is based on the theory of the numerical identification and microtest method and is suitable for identification of gram negative oxidase negative and glucose lactose fermenting bacilli according to their 9 biochemical properties
3. Nissui With Kanamycin Phenol Red Without L Glutamine and Sodium Bicarbonate Autoclavable 05900 08160 Powder Directions Dissolve 9 4 g of the medium in distilled water and adjust the volume to 1 000 mL Sterilize by autoclaving at 121 centigrade for 15 minutes Cool the sterilized medium to room temperature and add a proper amount of sterile 10 sodium bicarbonate solution pH will be 7 1 7 4 at 37 centigrade in an atmosphere of 5 CO in air by adding 12 5 22 0 mL of sodium bicarbonate solution This mixture should be stopper tightly and stored in a cool dark place At the time of use aseptically add 0 292 g of L glutamine sterilized and the desired amount of serum Summary Eagle s MEM Nissui 1 is a powdered medium prepared according to prescription published by Harry Eagle in 1959 Up to now such media were not autoclavable because of decomposition or deterioration of the constituents so that sterilization was performed by filtration using a membrane filter Nissui Pharmaceutical Co Ltd has succeeded in preparing an autoclavable powder medium This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired This medium has a growth promoting effect for cells such as Hela or L and other cell lines Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precaution
4. Nissui For Identification of Haemophilus and Neisseria 06635 06640 Reagent Features ID TEST NH 20 Rapid Nissui has been developed based on the theory of the microtest method and numerical classification With this method Haemophilus and Neisseria are identified rapidly in 4 hour incubation from positive or negative patterns of 20 different biochemical properties In this system results can be obtained which those of conventional methods with respect to the gram negative bacteria of Neisseria Pasteurella including Haemophilus or the bacteria of extremely weak growth such as Gardnerella or Eikenella Moreover identification includes simultaneous confirmation of B lactamase production by the B lactamase test 25 tests 100 tests EON 1 year Formula Kit component 06635 ID Test HN Plate 25 plates x 20 wells ID Test HN Broth Ze ME see tne an mL x 1 vial Result Form 13 sheets x 2 sets B lactamase Detection 3 mE x 1 vial Reagent Sterilized Liquid Paraffin 20 mL x 1 vial Kit component 06640 Kov cs Reagent 6 mL x 1 vial Nitrite Detection Test 6mLx4 vial Solution Nitrite Detection Test 6 mlx 14 vlal Solution II Zinc Powder 2 5 g x 1 vial Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD GAM Agar Nissui Gifu Anaerobic Medium Agar For common culture and susceptibility test Code ae 05420 Directions Suspend 74 0 g of the dehydrated m
5. Differentiation between pathogenic bacteria and nonpathogenic bacteria on the medium Code 300 g 2 6 Dry RT 3 years is easier than in case of Endo s or Drigalski s medium Even some organisms that cannot grow on SS Agar grow on the medium eNote eAseptic procedures should be taken for this medium contrary to those of SS Agar Formula Peptone 20 0 g Bile Salts 2 0 g Lactose 10 0 g Sodium Chloride 5 0 g Neutral Red 0 03 g Crystal Violet 0 001 g Agar 13 0 g pH 7 1 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD TSI Agar Nissui Triple Sugar Iron Agar Code 05103 Directions Suspend 61 4 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 4 mL into small test tubes sterilize by autoclaving at 121 C for 15 minutes Solidify in a slanted position with a generous butt Add 3 4 NaCl for vibrios Determinations Take the bacteria to be tested with a straight wire stab into the butt and smear on the slant Incubate at 37 C for 18 24 hours before interpreting the reaction Saccharolytic ability Dextrose fermenting organisms show the following changes the butt turns yellow while the slant shows no change of color Organisms fermenting both or either of lactose and saccharose change the color of both the butt and slant into yellow Gas production If gas is produced bubbles or cracks will be made in
6. Index Tissue Culture Medium 05909 Medium 199 NISSUI uunaanannnn gt nannnnnnnnnnnnnnnnunnunnnnnnunnnnann 19 13 05910 Ham s F12 Medium N ISSU unnnunnvnnnnnvuunae nnnnnnnnnnnnnnnnnn 19 14 05905 Hank s solution Nissui D 05906 Hank s solution Nissui Q aaaaaanaaaaaaaananannanananannnanan 19 15 Company Information Established April 6 1935 Head Office 3 23 9 Ueno Taito ku Tokyo 110 8736 Capital 4 449 844 000 yen Employees 334 2010 3 31 Associated Company Nippon Suisan Kaisha Ltd Distribution Culture Media and Diagnostics Pharmaceuticals and Health Food Supplements Export To Germany Thailand Korea Taiwan Hong URL Kong China United States Switzerland http www nissui pharm co jp Contact DIAGNOSTIC AGENT MARKETING DEPARTMENT INTERNATIONAL SALES GROUP TEL 81 3 5846 5701 FAX 81 3 5846 5629 In English Mikiko NISHIZAWA E mail m nishizawa nissui pharm jp In Chinese Koh SAI E mail k sai nissui pharm jp ix C NISSUI PHARMACEUTICAL CO LTD SBG Broth Base Nissui Selenite Brilliant Green Broth Base For isolation of Salmonelia Uninoculated 05006 Directions Suspend 19 7 g of the dehydrated medium and 4 0 g of sodium selenite in 1 000 mL of distilled water mix well to dissolve the medium Avoiding excessive heating keep at 100 C for 10 minutes Do not autoclave Distribute aseptically about 10
7. Link to User s Manual English Traditional Chinese Simplified Chinese Code a 06746 06747 Dried Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 20 25 C for 3 7 days Interpretations The medium contains chromogenic enzyme substrate X Phos that develops Blue color for many yeast and antibiotics that inhibit the growth of bacteria Mold forms the cottony colonies with characteristic color Some kinds of yeast do not develop Blue color on Compact Dry YM Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui VP Simple and Eas
8. NISSUI PHARMACEUTICAL CO LTD Index Dehydrate Media Salmonella 05006 SBG Broth Base NISSUI aaaaaaaaaaaaaaaaaa a aannnnnnnnnnnnnnnnnnnnanannnnn 1 1 05002 EEM Broth Nissui aaaaanaaanaaaaav nnnnnnnnannnnnnnannnnnnannannnnnanna naa 1 2 05009 Selenite Cystine Broth Base Nissui aaaaaaaaaaaaunnnnannnannnnnnnnn 1 3 05132 Tetrathionate Broth TT NISSUI aaavnvnananaaaaaaa annnnnnnnnnnnnnnnnnnnna 1 4 05130 Rappaport Vassiliadis Broth RV NiISSUI 2aavvvvvvvv nur 1 5 05131 Buffered Peptone Water BPW NISSUI aaaaaaaaannnnnnnnnnnuna 1 6 Enterobacteria Separation 05025 SSB Agar Nissui 05024 SSB Agar NISSU aauaaaaaaaanaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnanunna 2 1 05032 SS Agar with Sucrose Nissui 05033 SS Agar with Sucrose NISSUI auaaaaaannaaaanannnnnnunnnnnnnnnnnnnnnnnnnna 2 2 05021 SS Agar Nissui 05020 SS Agar N SSU auaaauanvna un gt nnnnr nnnnnnnnnnnnunnnnnunnnnnnnnnnnnnnnnannnnna nana 2 3 05040 DHL Agar Nissui 2uaaaavaaaanv nnnr nunnnunnnnnnnnnnnnnnnnnannanannnnann annan 2 4 05042 BTB Lactose Agar NISSUI aaaaaaaaanaunaaannannnnnunnnnnnnnnnnnnnnnnnnnnnnnna 2 5 05037 MacConkey Agar NISSUI aaaaaaaaaaanaaannnnnnnnnunnnnnnnnnnnnunnnnnnnnnnnnna 2 6 05103 TSI Agar Nissui auaaaanaaaaav nnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnanana nana 2 7 05140 X SAL Agar NISSU aaaaaaaaavaxaannnnnunnnunnnnnunn
9. Nissui Code an 05701 Directions Suspend 65 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes and use as a plate or a slant Granule Determinations Incubate at 37 C or at 25 C For the isolation of Candida incubate for 48 72 hours and in case of other fungi incubation for 5 days may sometimes be necessary Candida forms colonies with white opaque and wet swelling which gradually change to light brown Remarks Growth of Candida on the medium is so well that it can survive for a long period without morphological variations As the medium cannot inhibit the 300 g Dry RT 3 years contaminant microbes it is desirable to use the selective media such as Mycobiotic Agar and Candida GE agar simultaneously for the isolation Formula Peptone 10 5 g Dextrose 40 0 g Agar 14 5 g pH 5 6 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Corn Meal Agar Nissui Code 05702 Directions Suspend 17 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute the medium into Petri dishes and dry the surface before use Powder Determinations It is recommended to incubate at 25 C and 37 C It is also recommended to add Polysorbate 80 at the concentration of 1 for
10. is also used for the determination of lactose Back to the Index 11 2 o gt NISSUI PHARMACEUTICAL CO LTD EC Broth Nissui a Uninoculated S Code 05648 Directions In case of 1 mL or less of the specimen use the prescribed amount 37 0 g of the medium and in case of 10 mL or more of the specimen use twice the amount 74 0 g of the medium Suspend the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute 10 mL of medium into middle size of test tubes containing a fermentation tube or a Durham s tube Sterilize by autoclaving at 121 C for 15 minutes and cool down rapidly for use Do not use any tubes that contain bubbles in a fermentation tube or a Durham s tube Powder Remarks The medium is used for enumeration of coliform bacteria in water milk and seafood products When gas is evolved after incubation at 100 g 11 3 Dry RT 3 years 37 C for 48 hours determine coliform to be positive and determine Escherichia coli to be positive in case of evolution of gas after incubation at 44 5 C The medium inhibits the growth of Enterococcus Formula Peptone 20 0 g Lactose 5 0 g Bile Salts 1 5 g Dipotassium Phosphate 4 0 g E Dihydrogen 15g Sodium Chloride 5 0 g pH 6 9 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Desoxycholate Agar Nissui Code 05636 Directions Suspend 45 0 g
11. 0 01 ug 0 1 pg tube 2 mL 3 years Code an Suspend 7 7 g of the dehydrated medium in about 80 90 mL of distilled water mix well and heat to dissolve the medium and cool Storage down the solution After adjusting the pH to Cap tightly and keep at cool place 2 7 1 add distilled water to make the total 10 C volume 100 mL Distribute into the test tube and double the Formula total volume with the distilled water after As directed in N F XIV 1975 adding the sample Sterilize by autoclaving pH 7 1 0 1 at 121 C for 5 minutes Inoculate the strain for culture at 37 C for 16 24 hours Back to the Index Incubate 72 hours for the purpose of acidimetry Remarks Strain to be used is Lactobacillus arabinosus strain 17 5 ATCC 8014 available also from Nissui Range of response 0 02 pg 0 2 ug tube 10 mL 13 7 o gt NISSUI PHARMACEUTICAL CO LTD Folic Acid Assay Medium Nissui 05814 Powder Directions 0 2 ng 2 ng tube 5 mL Suspend 11 4 g of the dehydrated medium 0 05 ng 0 5 ng tube 2 mL in about 50 mL of distilled water mix well 3 years Code an and heat to dissolve the medium and cool Storage down the solution After adjusting the pH to Cap tightly and keep at cool place 2 7 1 0 1 add distilled water to make the 10 C total volume 100 mL Distribute into the test tube and double the Formula total volume with the distilled water after As directed in AOAC 12 Ed 1975
12. 0 05 mg 0 025 mg 0 025 mg 0 025 mg 0 025 mg 0 01 mg 0 01 mg 0 1 mg 0 05 mg 0 01 mg 0 1 mg 0 01 mg 0 9 mg 0 5 mg 10 0 mg 0 3 mg 0 3 mg 0 3 mg 0 3 mg 0 3 mg 0 2 mg 0 5 mg 5 0 mg 0 2 mg 0 05 mg 50 0 mg 1 0 mg 1 000 0 mg 6 800 0 mg 400 0 mg 200 0 mg 97 7 mg 102 9 mg 0 72 mg 6 0 mg Ham s F12 Medium Nissui Without Sodium Bicarbonate Powder 05910 Directions Dissolve 10 6 g of the medium in distilled water and adjust the volume to 1 000 mL Don t heat over 37 C to dissolve Add a proper amount of sodium bicarbonate pH will be 7 1 7 4 at 37 C in an atmosphere of 5 CO in air by adding 1 2 2 0 g sodium bicarbonate Sterilize by filtration immediately To avoid excessively raising the pH level of the medium it is advantageous to reduce the pH of the medium by gassing with CO before filtration Rehydrated medium will keep 1 2 months when it is stored in a freezer Aseptically add the desire amount of serum Summary Ham s F12 Medium Nissui is a powdered medium prepared according to the prescription published by Richard G Ham in 1965 and made by special manufacturing This medium is recommended for proliferation of mammalian cells as clones especially Chinese Hamster cells It is also recommended for primary cultures Storage and Expiration Close tightly and store at 2 5 C in a dark dry place May be stored up to one 1 year Precautions Do n
13. Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Malt Agar Nissui Code 05706 Directions Suspend 45 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into the test tubes sterilize by autoclaving at 121 C for 15 minutes and use after solidifying in a slanted position or in the butt Powder Remarks The medium is used for the preservation of Saccharomyces carlsbergensis that is used for analysis of Vitamin B It is also used widely for the cultivation of common lactobacilli yeast and fungi 100 g Dry RT 3 years Formula Malt Extract 25 0 g Agar 20 0 g pH 5 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Potato Dextrose Agar Nissui 05709 Directions Suspend 39 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Use as the plate or the pour plate method Incubate at about 25 C is desirable For mixing and dilution method distribute the medium into Petri dishes contained the test specimen in advance Granule Remarks The medium is used for the isolation of yeast and fungi from powdered milk or processed milk products To inhibit the growth of other bacteria for the purpose of enumerating the numbers of yeast and fungi adjust the above pH 6 0 to pH 3 5 0 1 add sterilized 10 aqueous solutio
14. Fluid Nissui Code an 05601 Directions Suspend 29 3 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute necessary amounts of medium into test tube sterilize by autoclaving at 121 C for 20 minutes and immediately cool down for use The prepared medium should not be used if more than one thirds of the upper part of the medium turned carmine Add the test sample mix thoroughly with the medium and incubate at 30 35 C at least for 14 days If it is difficult to determine the result transfer a part of the culture into newly prepared medium and sub culture Incubate at 30 35 C at least for 7 days before a final determination Granule Remarks The medium is most suitable for sterility 300 g Dry RT 3 years testing of injections Formula L Cystine 0 5 g Sodium Chloride 2 5 g Dextrose 5 0 g Yeast Extract 5 0 g Peptone 15 0 g Sodium Thioglycollate 0 5 g Resazurin 0 001 g Agar 0 75 g pH 7 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD TGC Medium without Indicator Liquid Nissui Code 05610 Powder 300 g Dry RT 3 years Directions difficult to use TGC Medium due to turbidity or high viscosity of the test samples As it does not contain agar it is easy to mix the samples with the medium Suspend 28 5 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute the
15. Keep dry at 4 10 C Do not freeze Formula Components Yeast Extract 2 5g Peptone 5 0 g Dextrose 1 0 g Agar 15 0 g pH 7 0 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui XM G Agar XM G Simple and Easy Stamp Medium for Food Hygiene test Escherichia coli and Coliforms Code ne 06776 30 Plates Soli 06775 i 100 Plates Hi a Directions Formula Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Com ponents In SES g l Take off the cap of Food Stamp and gently press the medium against Peptone 10 0 g the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts Sodium Pyruvate 1 0 g of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing L Tryptophan 1 0 g Incubate at 35 C for 20 2 hours D Sorbitol 1 0 g Coliform group decompose X GAL colorimetric enzyme substrate in the medium to bring out blue blue green color byB galactosidase Sodium Chloride 5 0 g produced by Coliforms Sodium dihydrogen Phosphate 2 2 g Growth of all other bacteria will be inhibited and they develop only 7 white colonies even are they grow Disodium Phosphate 2 7 g Interpretations Potassium nitrate 1 0 g Count all pink red purple color colonies grown on the surface a
16. Nissui Identification Test Kit for Glucose fermentative Gram Negative bacilli eee IH bot YP PG no RHA WaN 06626 06615 EB Plate 06616 EB Broth 06628 EB Reagent Features of ID Test EB 20 Identification of bacteria is very complicated and there are so many biochemical test need to be done ID Test EB 20 is based on the theory of the numerical classification method which could receive the combination with 20 kinds of biochemical reactions Only small amount of sample 0 1 mL is needed for each test item Also the kit is stable for 1 year at room temperature Purpose of ID Test EB 20 Identification of Glucose fermentative Gram Negative bacilli Directions for operation of the kit Streak samples onto blood agar or other isolation medium After incubation of the medium confirm colonies will be tested are Gram Negative bacilli and Glucose fermentative The test target bacteria shall be proliferated on enrichment medium Fish colonies from enrichment medium for adjustment the concentration Suspension that corresponds to No 1 McFarland turbidity standard Inoculate 0 1 mL of above suspension into each well 20 wells Layer 3 5 drops of sterilized liquid paraffin on 8 certain wells and incubate place the cover on the plate and incubate at 37 C for 18 20 hours After incubation add 1 drop of 10 Ferric Chloride Test solution PPA Kovacs Reagent IND 6 o naphthol Test 3 8
17. Thiamin Hydrochloride 1 0 mg Cyanocobalamin 0 005 mg Sodium Chloride 6 000 0 mg Potassium Chloride 400 0 mg Calcium Nitrate 69 5 mg Disodium Phosphate 801 0 mg Magnesium Sulfate 48 8 mg Glucose 2 000 0 mg L Glutamine 300 0 mg Phenol Red 5 0 mg 19 10 Back to the Index RPMI 1640 Medium Nissui Without L Glutamine and Sodium Bicarbonate Autoclavable Powder 05918 Directions Dissolve 10 2 g of the medium in distilled water 100 g Formula 1 Year In 20 2 g L and adjust the roume to 1 000 mL Sterilize by L Arginine Hydrochloride 240 0 mg autoclaving at 121 C for 15 minutes Cool the L Asparagine H O 56 8 mg sterilized medium to room temperature and add a z i M L Asparatic Acid 20 0 mg proper amount of sterile 10 sodium bicarbonate L Cysteine Hydrochloride H O 72 9 pH will be 7 1 7 4 at 37 C in an atmosphere of LGI eA id rog 20 0 mg 5 CO in air by adding 18 30 mL of sodium utamic Aci a bicarbonate solution Aseptically add 0 3 g L BUL au Seas glutamine and the desire amount or serum Sodium Succinate 6H20 164 0 mg S Glutathione 1 0 mg ummary Glycine 10 0 mg RPMI 1640 is a medium for suspension culture L Histidine Hydrochloride H O 20 3 mg investigated by George E Moore former director L Hydroxyproline 20 0 mg of Roswell Park Memorial Institute Up to now k L Isoleucine 50 0 mg such media were not autoclavable because of s L Leucine 50 0 mg decomposition or deteriorat
18. identified to have a high possibility of triggering food allergies when foods contain more than a predetermined amount of g mL or ug g of the specified raw ingredients as of April 2002 Similarly as of June 2008 shrimps prawns except lobsters and crawfishes and crabs are added to Food Sanitation Law In addition to clearly labeling the above mentioned food products screening of such products is increasingly becoming important in order to confirm the amount of proteins contained in the raw ingredient or in order to confirm the content due to the mixing of these raw materials with other foodstuffs and contamination on production lines FA test Immunochromato Crustacea is a detection kit for crustacean derived protein in foodstuffs based on a lateral flow system with 20 Tests 1 Year simple operation and results obtainable in a short period of time Product Features FA test can detect crustacean derived tropomyosin in foodstuffs FA test is a high specific assay system due to a high specific monoclonal antibody FA test can detect crustacean derived tropomyosin in foodstuffs with simple and rapid operation FA test is widely applicable to samples ranging from raw ingredients to processed foods Formula 1 Test device 20 Tests 2 Condensed extraction buffer 10 fold condensate SIMS deade 3 Sample dilution buffer 100 mL x 1 Bottle Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Eagle s MEM
19. mL amounts in to sterilized test tubes Inoculate about 1 g of the specimen such as feces Mix well and incubate at 37 C for 18 24 hours Take a loopful of the culture and then inoculate on a plate of the selective medium Use sodium selenite of high quality Remarks The medium inhibits the growth of Alcaligenes Pseudomonas Proteus and other bacteria that grow on the selenite medium and permits the growth of Salmonella S typhi and S paratyphi A do not grow well Powder Code an 100 g 1 1 Salmonella 2 years in the medium SBG Sulfa Medium is more suitable than this medium for the isolation of Salmonella from eggs and egg products Storage Keep dry and cool place to prevent powder product and prepared solution form color fading Formula DEO Yeast Extract 5 0 g Peptone 5 0 g Mannitol 5 0 g Sodium Taurocholate 1 0 g Dipotassium Phosphate 2 65 g Potassium Dihydrogen Phosphate 102E Brilliant Green 0 005 g pH 7 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD EEM Broth Nissui Enterobacteriaceae Enrichment Mannitol Broth for preliminary culture of Salmonella A A Uninoculated Salmonella 05002 Powder 100 g Dry RT 3 years Directions Formula hi E T in 1 000 mL of distilled water mix well to dissolve the medium Distribute adequate Peptone 10 0 g amounts into sterilized test tubes or sterilized flask and then heat them at 100 C for 30 minutes Avoid excessi
20. the butt and the production of hydrogen sulfide changes the butt into black Addition of cytochrome oxidase test reagent by dropping it onto the colonies causes them to turn blue within a minute or two the positive reaction Powder 100 g 2 7 Dry RT 3 years indicates the colonies are those of V cholerae Remarks The medium is a composite medium for the differentiation of Enterobacteriaceae and vibrios and permits simultaneous observation of fermentation of dextrose lactose and saccharose gas production and the hydrogen sulfide production Formula In 61 4 g L Meat Extract 5 0 g Sodium Chloride 5 0 g Peptone 15 0 g Lactose 10 0 g Sucrose 10 0 g Dextrose 1 0 g Ferric Citrate 0 2 g Sodium Thiosulfate 0 2 g Phenol Red 0 02 g Agar 15 0 g pH 7 3 Back to the Index C NISSUI PHARMACEUTICAL CO LTD X SAL Agar Nissui For isolation of Salmonella causing food poisoning Code an 05140 Directions Suspend 68 2 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL amounts to Petri dishes and dry the surface of the medium before use Do not autoclave Granule Determinations Inoculate the specimens heavily and incubate at 33 37 C for 18 24 hours Pathogenic bacteria form semitransparent colonies Lactose fermenters form pink or red colonies Some strains of Proteus produce hydrogen sulfide and turn black Proteus gr
21. they form only small colonies Blood agar prepared by adding blood to the medium in a ration of 5 is especially useful as the selective medium for the types of streptococci and anaerobic bacteria including obligate anaerobes and some of clostridia Formula Peptone 15 0 g Soya Peptone 5 0 g Sodium Chloride 5 0 g Phenylethyl Alcohol 2 5 g Agar 15 0 g pH 7 3 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Salt Egg Yolk Agar Base Nissui Code 05238 Granule 300 g Dry RT 3 years Directions medium in ring shapes from the lower part of the colonies In 18 24 hours of incubation pearly lustrous rings like those made by oil drops falling upon the water surface are formed around the colonies on the medium Therefore even the pearly lustrous rings alone can serve as a means of differentiation of coagulase positive staphylococci Colonies of coagulase negative staphylococci are rather opaque and do not form pearly lustrous rings or opacify the Suspend 115 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Cool down to approximately 50 60 C Add an egg yolk suspension in a ratio of 10 with thorough mixing Distribute about 20 mL of the medium into Petri dishes Dry the surface of the plate before use i For preparation of an egg yolk suspension meam aseptically add one egg yolk to 30 ml of 10 For
22. 3 years Range of response 0 01 ng 0 2 ng tube 5 mL Incubation temperature should be constant within 0 5 C Pay at most attention to preserve the strains Storage Cap tightly and keep at cool place 2 10 C Formula As directed in U S P XIX 1975 pH 6 2 0 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Pyridoxine Assay Medium Nissui 05815 Directions Suspend 13 0 g of the dehydrated medium in about 80 90 mL of distilled water mix well and heat to dissolve the medium and cool down the solution After adjusting the pH to 5 2 add distilled water to make total volume 100 mL Distribute into the flask or test tube and double the total volume with the distilled water after adding the sample Sterilize by boiling at 100 C for 10 20 minutes Inoculate the strain for culture at 30 C for 16 24 hours Powder Code Remarks Strain to be used is Saccharomyces uvarum carlsbergensis strain 4228 ATCC 9080 available also from Nissui Range of response 2 5 ng 25 ng tube 10 mL 3 years In case of incubating in the test tube all test tubes should be similarly slanted more than 25 degree at the angle Storage Cap tightly and keep at cool place 2 10 C Formula Improved formula of Atkin et al method pH 5 2 0 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Niacin Assay Medium Nissui 05816 Powder Directions 0 01 pg 0 1 pg tube 5 mL
23. 3 years blue blue green color by B galactosidase produced by coliform group Formula Peptone 15 0 g Yeast Extract 5 0 g Sodium Pyruvate 1 0 g Sodium Chloride 5 0 g Disodium Phosphate 2 0 g Potassium nitrate 1 0 g Sodium Lauryl Sulfate 0 15 g S bromo 4 chloro S Indoly 0 15 g D galactopyranoside X GAL Agar 15 0 g pH 7 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD XM G Agar Nissui Test for Coliform group and E coli Code an 05632 Directions Suspend 39 3 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute necessary amounts of medium into Petri dish Remarks The medium is used for the screening test for E coli and coliform group E coli has a B glucuronidase that decomposes the substrate of X GLUC to produce blue dye For the meanwhile Coliform group have a galactosidase that decomposes the substrate of MAGENTA GAL to produce red dye E coli has both enzymes and may produce a blue to blue purple color Since E coli 0157 does not have a B glucuronidase they are identified as a Coliform group Incubate at 35 37 C for 20 2 hours Over time incubation may foster growth of microorganism other than E coli and Coliform group Red color Granule 300 g 11 7 Dry RT 3 years may be observed if the sample contains lactobacilli that has also a B galactosidase
24. 8 0 g Sodium Thiosulfate 8 5 g Disodium Phosphate 7 5 g Ferric Citrate 3 0 g Sodium Chloride 10 0 g Agar 15 0 g Water Blue 0 2 g Cresol Red 0 02 g pH 9 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Polymyxin Mannose Tellurite Agar Base Nissui Isolation of El Tor cholera vibrio Code an 05208 Directions Suspend 60 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Cool the medium to about 60 C add 1 mL of 0 1 aqueous solution of potassium tellurite and mix well Distribute about 20 mL of the medium into Petri dishes No sterilization such as autoclaving is required Dry the surface of the medium before use After addition of 0 1 aqueous solution of potassium tellurite do not reheat Determinations Inoculate the specimens directly or after enrichment culture and incubate at 37 C for 18 24 hours El Tor cholera vibrio forms yellow colonies with a brown center due to the fermentation of mannose Other vibrios are mostly inhibited on the medium but some organisms may grow on it However their colonies are smaller than those of El Tor cholera vibrio and have blue color Thus the differentiation of El Tor cholera vibrio is easy As a few strains of NAG non agglutinable vibrios form yellow colonies careful observation is needed Remarks The medium is designed to detect El Tor cholera vibrio from the specimens that are presumed to
25. 8 g powders in distilled water and adjust the volume to 1 000 mL After dissolving completely add a proper amount of sodium bicarbonate pH will be 7 3 7 6 at 37 C in an atmosphere of 5 CO in air by adding 0 2 0 35 g sodium bicarbonate Sterilize by filtration immediately To avoid excessively raising the pH level of the medium it is advantageous to reduce the pH of the medium by gassing with CO before filtration Storage and Expiration Close tightly and store at 2 5 C in a dark dry place May be stored up to two 2 year 100 g 19 15 2 Years Formula Sodium Chloride 8 000 0 mg Potassium Chloride 400 0 mg Disodium Phosphate 47 9 mg Potassium Dihydrogen Phosphate 600mg Magnesium Sulfate 48 8 mg Magnesium Chloride 46 8 mg Calcium Chloride 140 0 mg Glucose 1 000 0 mg Back to the Index C NISSUI PHARMACEUTICAL CO LTD
26. For differential medium of coagulase positive staphylococci add egg yolk solution to the medium To prepare the 10 salt egg yolk agar medium suspend 111 0 g of the dehydrated medium in 900 ml of distilled water sterilize by autoclaving and cool down to 50 C to which add 100 ml of egg yolk solution mixture of about 20 g of egg yolk and 80 ml sterilized physiological saline solution Formula Peptone 10 1 g Meat Extract 6 5 g Sodium Chloride 71 3 g Mannitol 9 2 g Phenol Red 0 023 g Agar 13 8 g pH 7 4 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Phenylethyl Alcohol Agar Nissui Code 05246 Directions Suspend 42 5 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute 20 mL of the medium into Petri dishes Use the medium within a day of its preparation and do not preserve it The medium once solidified should not be dissolved again For isolation of anaerobic bacteria the incubation time should usually be 48 hours and 72 hours at the longest Powder Remarks The medium can be used for isolation of gram positive cocci such as streptococci and staphylococci from the materials in which gram negative bacteria such as Escherichia coli and Proteus coexist The gram negative bacteria are mainly 100 g 6 3 Dry RT 1 year inhibited on the medium Even if they grow on it
27. Formula TEER Peptone 10 0 g Sodium Pyruvate 1 0 g L Tryptophan 1 0 g D Sorbitol 1 0 g Sodium Chloride 5 0 g Sodium dihydrogen Phosphate 2 2 g Disodium Phosphate 2 7 g Potassium nitrate 1 0 g Sodium Lauryl Sulfate 0 2 g 5 bromo 4 chloro 3 indolyl B 0 1 g D glucuronide X GLUC 5 bromo 6 chloro 3 indolyl B D 0 1 g galactopyranoside MAGENTA GAL Agar 15 0 g pH 7 0 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Blue Light Broth Nissui Rapid test for Coliform group and E coli Code an 05607 Directions Suspend 17 4 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute necessary amounts of medium into test tube Sterilize by autoclaving at 121 C for 15 minutes Granule Remarks The medium is used for the test for coliform group and E coli in foodstuffs or water Coliform group change the medium blue to blue green after incubation at 35 37 C for 24 hours Fluorescent is observed when the positive medium is exposed to UV light 365 nm if E coli was present X GAL colorimetric enzyme substrate in the medium is decomposed to bring out blue blue green color by B galactosidase produced by coliform group MUG in the medium is decomposed byB Glucuronidase to be produced by E coli to 300 g Dry RT 3 years isolate a fluorescence substance of 4 Methyl Umbelliferone Formula Peptone 5 0 g Sodium Chloride 5 0 g Sod
28. L cysteine are being added to the composition the ability of the medium with supports the growth of lactobacilli has been improved As Lactobacillus bulgaricus invariably forms yellowish colonies its detection and determination are easy Powder 300 g Dry RT 2 years Color of the medium may be changed to purple when incubation is prolonged as the medium becomes alkaline due to the growth of bacteria Bifidobacterium does not grow on the medium and it is recommended to incubate it anaerobically with BL Agar medium Formula Yeast Extract 2 5 g Peptone 5 0 g Dextrose 1 0 g Polysorbate 80 1 0 g L Cysteine 0 1 g Brom Cresol Purple 0 06 g Agar 15 0 g PH 7 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD CVT Agar Nissui Viable counting for psychrophilic microorganism Code 05625 Powder 100 g Dry RT 3 years Directions is dissolved Suspend 23 5 g of the dehydrated medium Whole eu niay change to red when in 1 000 mL of distilled water mix well and the sample contains certain products that heat to dissolve the medium Do not reduce TTC autoclave Formula dealt The medium is developed by Olson to measure the viable count of psychrophilic Yeast Extract 2 58 microorganism in milk and dairy products Peptone 5 0 g mainly non fermentative gram negative rod such as Pseudomonas Dextrose 1 0 g After incubation at 20 25 C for 48 72 Crystal Violet 0 002 g hours gram negative rods
29. Quick ID GN Nissui Reagent aaaaaaaaaaaaa aanann nnnnnnnnnnnnnnnna 3 11 Vibrio parahaemolyticus cholera 05215 Salt Polymyxin Broth NISSUI aaaaaaaanaaaaan nnnnnannnnnnnnnnnnnnnnnnanna 4 1 05206 Alkaline Peptone Water NISSUI aaaaaaaaaaa nn nnnnnnnnnnnnnnnnnnnuna 4 2 05201 Vibrio Agar NISSU aaaaaaaaaaanaannannnnnnnnnnnnnnnnnnnnanunnnnnnnnnnnnnnnnnnnnna 4 3 05208 PMT Agar Base Nissui aaaaaaananaaaaannnnnnnnnnnnnnnunnnnnnnnnnnnunnnnnnnnnna 4 4 05204 Thiosulfate citrate bile Saccharose Agar Nissui 4 5 05135 X VP Agar N SSU u11vsmmvvva ss asuass a aa 4 6 Non fermentative Gram negative rod 05220 NAC Agar ISS neitiid inisa 5 1 05221 Cetrimide Agar NISSUI aaauanaaaaaaanannnnnnnnnunnnnnnnnnnnnnnnnnnnnnanunnnnna 5 2 06629 ID Test NF 18 Nissui 06617 ID Test NF 18 Nissui NF Plate 06618 ID Test NF 18 Nissui NF Broth 06631 ID Test NF 18 Nissui NF Reagent ccssssssesssseeeees 5 3 Staphylococci 05234 Staphylococcus Medium No 110 NiSSUI aaaaanaanaanaaannnnnnnn 6 1 05236 Mannitol Salt Agar NISSUI aaaaazaanaaaaaann nannnnnnnnnnnnnnnanannnnnnununa 6 2 05246 PEA Agar NISSUI aaaaaaanaaannnnnnnnnnnnnnnnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna 6 3 05238 Salt Egg Yolk Agar Base NISSUI aaaaaaaanaaaaaaann nunnnnnnnnnnnnnnnna 6 4 06637 N D Test SP 18 Nissui 06613 N ID Test SP 18 Nissui SP Plate 066
30. Two specimens will be simultaneously identified with one plate Major members of Enterrobacteriaceae will be identified with 18 hours incubation Package is so designed that many specimens will be economically tested Purpose of ID Test EB 9 Identification of major intestinal bacilli Directions for operation of the kit Streak the test specimen onto BTB Lactose Agar Nissui Plate or MacConkey Agar Nissui Plate or DHL Agar Nissui Plate over and culture for isolation of single colonies Confirm that the test bacteria on the siolation culture medium are gram negative and also oxidase negative rods and that they are glucose lactose fermentative These can be confirmed with Cytochrome oxidase test and TSI agar Exclude other bacteria Culture the test bacterium on nutrient agar Preparation of Bacterial Suspension Collect the test bacteria multiplied by culture on a loop and suspend them in one vial of ID Test Adjust the concentration of bacteria to a level No 1 of McFarland Inoculation of Bacterial Suspension Draw up the prepared bacterial solution suing a sterilized capillary piptte and inoculate into each well of the ID test Plate up to the step 0 1 mL After inoculation add 3 5 drops of sterile liquid paraffin on top of the following 5 items ARA INO LDC ODC and URE to form a stratified layer in order to isolate them from the air EB Reagent 3 9 50 tests 100 tests a 1 year Place the cover on t
31. When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose Formula in 9 6 g for 1 liter 1 vial contains 0 3 g sterile and lyophilized L glutamine Back to the Index 19 6 C NISSUI PHARMACEUTICAL CO LTD Dulbecco s Modified Eagle Medium Nissui 1 With L Glutamine Without Sodium Bicarbonate Powder 05915 Directions Dissolve 10 0 g of the medium in distilled water and adjust the volume to 1 000 mL Add a proper amount of sodium bicarbonate pH will be 7 1 7 4 at 37 in an atmosphere of 5 CO in air by adding 1 0 1 8 g sodium bicarbonate Dissolve completely and immediately sterilize by filtration To avoid excessively raising the pH level of the medium it is advantageous to reduce the pH of the medium by gassing with CO before filtration This mixture should be stopped tightly and stored in a cool dark place At the time of use aseptically add the desired amount of serum Summary Dulbecco s Modified Eagle Medium Nissui is a powdered medium prepared on the basis of research by R Dulbecco et al and is specially manufactured Compared with Eagle s MEM this medium contains about twice the concentration of amino acids and about four times the
32. concentration of vitamins It contains Glycine and L serine which are nonessential amino acids Pyruvate which is important for glycolysis and a very small amount of ferric ion It is nutritious for the growth of cultured cells and is widely used for research on viruses primary cell cultures and single cell cultures Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose 100 g 1 Year Formula Sodium Chloride 6 400 0 mg Potassium Chloride 400 0 mg Calcium Chloride 200 0 mg Magnesium Sulfate 97 7 mg Sodium Dihydrogen Phosphate 2H O S U Ferric Nitrate 9 HO 0 1 mg Glucose 1 000 0 mg Sodium Pyruvate 110 0 mg L Arginine Hydrochloride 84 0 mg L Cystine Dihydrochloride 62 6 mg Glycine 30 0 mg L Histidine Hydrochloride H O k L Isoleucine 104 8 mg L Leucine 104 8 mg L Lysine Hydrochloride 146 2 mg L Methionine 30 0 mg L Phenylalanine 66 0 mg L Serine 42 0 mg L Threonine 95 2 mg L Tryptophan 16 0 mg L Tyrosine Disodium Salt 89 5 mg L Vali
33. crustacean tropomyosin specific monoclonal antibody Product Features FA test ElA Crustacea Nissui can determine crustacean derived proteins in foodstuffs precisely FA test Extraction Solution Nissui 1 Package Shelf Life 96 Tests 18 3 1 Year FA test ElA Crustacea Nissui is a high specific assay system due to a high specific monoclonal antibody FA test ElA Crustacea Nissui can cover a wide measurement range of 0 3 to 20 ppm FA test ElA Crustacea Nissui is widely applicable to samples ranging from raw ingredients to processed foods Formula 1 Antibody fixed micro titer plate 1 Plate 96 wells 2 Standard solution 50 ng mL 1 2 mL x 2 Vials 3 Dilution buffer 100 mL x 1 Bottle 4 Enzyme labeled antibody 13 mL x 1 Vial 5 Enzyme substrate solution 13 mL x 1 Vial 6 Stopping solution 1N H SO 13 mL x 1 Vial 100 mL x 1 Bottle 55 mL x 1 Bottle 55 mL x 1 Bottle 50 mL x 1 Bottle 7 Washing Solution x10 Extraction Solution Components 08622 A Extraction solution A x20 B Extraction solution B x20 C Extraction solution C x20 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD FA test Immunochromato Crustacea Nissui O M o Code 08623 Introduction Amendments to Food Sanitation Law in Japan have rendered it mandatory to label 5 specific raw food materials Eggs Milk Wheat Buckwheat and Peanuts
34. enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 24 hours Interpretations Count all colonies on the surface Storage Keep dry at 4 10 C Do not freeze Formula Sodium Desoxycholate 1 0 g Peptone 10 0 g Ferric AMmonium Citrate 2 0 g Sodium Chloride 5 0 g Dipotassium Phosphate 2 0 g Lactose 10 0 g Neutral Red 0 033 g Agar 15 0 g pH 7 2 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui X GAL Agar XGAL Simple and Easy Stamp Medium for Food Hygiene test Coliform Code ne 30 Plates 06764 06763 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 24 hours Coliform group decompose X GAL colorimetric enzyme substrate in the medium to bring out blue blue green color by B galactosidase produced by Coliforms Growth of all other bacteria
35. in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Maintain the medium at about 50 C Distribute about 20 mL into Petri dishes contained test samples in advance and mix well Incubate at 35 1 C for 48 3 hours Granule Remarks The medium is applied for the following purposes enumeration of bacteria in milk dairy products soft drink powder chipped ice ice cream oysters frozen fishes and seafood products and others Also for enumeration of thermo resistant bacteria Spores in sugar starch and spices to be used for the products of fish and meat 300 g Dry RT 3 years Formula Yeast Extract 2 5 g Peptone 5 0 g Dextrose 1 0 g Agar 15 0 g pH 7 0 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Plate Count Agar with BCP Nissui Plate Count Agar with Brom Cresol Purple For enumeration of the numbers of lactobacilli Code 05622 Directions Suspend 24 7 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Maintain the medium at about 50 C Distribute about 20 mL into Petri dishes contained the test sample in advance mix well and incubate at 35 37 C for 72 3 hours Remarks Lactobacilli grown on the medium form colonies with yellowish peripheries in the depth and on the surface of the medium As polysorbate 80 and
36. medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 20 25 C for 2 5 days Solid Interpretations Fungi develop characteristic fluffy colonies on the surface 100 Plates 15 10 All characteristic colonies should be counted for evaluation Storage Keep dry at 4 10 C Do not freeze Formula Peptone 10 5 g Dextrose 40 0 g Agar 14 5 g pH 5 6 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui Potato dextrose Agar with Chloramphenicol PDA Simple and Easy Stamp Medium for Food Hygiene test Food poisoning Fungi Code nee 30 Plates 06755 06754 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put th
37. of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute an adequate amount into test tubes or flasks and sterilize by autoclaving at 115 C for 15 minutes Powder Remarks The medium is a modification of Mueller Hinton medium and does not inactivate sulfa drugs and any other antibiotics The medium is used when fluid medium is preferable Storage The medium is extremely hygroscopic and stored in the desiccator closed tightly 300 g Dry RT 3 years Formula Casamino Acid 16 5 g Heart Extract Powder 3 0 g Soluble Starch 1 5 g Dextrose 2 0 g L Tryptophan 0 05 g L Cystine 0 05 g Biotin 5 ug pH 7 4 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Heart Infusion Agar Nissui Code ae 05503 Directions Suspend 40 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute into Petri dishes or test tubes according to the purpose Granule Remarks Since the medium has an excellent ability to support the growth of bacteria it is suitable for the cultivation of many fastidious pathogenic bacteria Since E coli Salmonella and Shigella which frequently mutate are stable during the preservation on this medium it is useful for the test of their antigenicity and virulence 300 g Dry RT 3 years Formula Components Heart
38. of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute necessary amounts of medium into Petri dish Granule Remarks The medium is used for an isolation of E coli 0157 based on the nature that 0157 does not ferment sorbitol while other E coli do E coli 0157 develops a semitransparent colony after incubation at 37 C for 18 20 hours For the meanwhile other E coli develop a redbrick color colony with light peach or redbrick zone of desoxycholic acid precipitated Growths of gram positive coccus are inhibited 300 g Dry RT 3 years Formula Components Bile Salt 1 0 g Peptone 19 0 g D Sorbitol 10 0 g Sodium Chloride 5 0 g Crystal Violet 0 001 g Neutral Red 0 03 g Agar 15 0 g pH 7 2 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD EF Agar Base Nissui Appended reagent TTC or Isolation of Enterococcus Code an 05679 Directions Suspend 54 5 g of the dehydrated medium together with 0 25 g of Sodium azide in 1 000 mL of distilled water mix well and heat to dissolve the medium Keep at around 60 C and then add 10 mL of 1 5 TTC solution Mix well and distribute the medium into Petri dishes 1 5 TTC solution Dissolve 0 15 g of TTC appended to the medium in 10 mL of distilled water and Powder store in brown bottle in dark and cool place Remarks Th
39. of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose Back to the Index 100 g 1 Year Formula Sodium Chloride 6 800 0 mg Potassium Chloride 400 0 mg Sodium Dihydrogen Phosphate 115 0 mg Magnesium Sulfate 93 5 mg Calcium Chloride 200 0 mg Glucose 1 000 0 mg L Arginine Hydrochloride 126 0 mg L Asparagine H O 30 0 mg L Asparatic Acid 27 0 mg L Alanine 18 0 mg L Cysteine Hydrochloride H O 31 4 mg L Glutamic Acid 30 0 mg Glycine 15 0 mg L Tyrosine 36 0 mg L Histidine Hydrochloride H O 42 0 mg L Isoleucine 52 0 mg L Leucine 52 0 mg L Lysine Hydrochloride 73 0 mg L Methionine 15 0 mg L Phenylalanine 32 0 mg L Proline 23 0 mg L Threonine 48 0 mg L Serine 21 0 mg L Tryptophan 10 0 mg L Valine 46 0 mg Succinic Acid 75 0 mg Sodium Succinate 6 H O 100 0 mg Choline Bitartrate 1 8 mg Folic Acid 1 0 mg Inositol 2 0 mg Nicotinamide 1 0 mg Calcium Pantothenate 1 0 mg Pyridoxine Hydrochloride 1 0 mg Riboflavin 0 1 mg Thiamin Hydrochloride 1 0 mg Biotin 0 02 mg Sodium Pyruvate 110 0 mg Cyanocobalamin 0 1 mg Kanamycin 60 0 mg Titer Phenol Red 6 0 mg o gt NISSUI PHARMACEUTICAL CO LTD Medium 199 Nissui Without Sodium Bicarbonate Form Code Powder 05909 Directions Dissolve 9 5 g of the medium in distilled water and adjust
40. pH 5 2 0 2 Back to the Index amp NISSUI PHARMACEUTICAL CO LTD Buffered Peptone Water BPW Nissui Buffered Peptone Water for Pre culture of Sa monella Code an 05131 Directions Suspend 20 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute adequate amounts into sterilized test tubes or sterilized flask and then autoclave at 121 C for 15 minutes Add 1 10 volume of the homogenized specimen i e 1 mL homogenized specimen put into 10 mL broth into the broth and stir them well Incubate at 37 C for 20 24 hours Finally take one loop of the culture and subculture on an adequate selective medium ex Compact Dry SL SS agar MLCB Agar For liquid Egg sample add 0 2 g L cystein or 64 mg FeSO 7H 0 in 1 liter of Broth Powder Remarks The medium is suitable for pre culture of 300 g Dry RT 3 years Salmonella in food Formula Components Peptone 10 0 g Sodium Chloride 5 0 g Monopotassium Phosphate 155 Potassium Dihydrogen Phosphate 1 58 pH 7 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD SSB Agar Nissui Salmonella Shigella Brom Cresol Purple Agar Code 300 g 05025 05024 Directions Suspend 60 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into Petri dishes without sterilizing and dry the surface of the
41. permits the growth of Candida specifically 2 Nitrofuran derivative markedly or completely inhibits gram negative bacilli Therefore the medium facilitates the detection of Candida Note It is necessary to smear the specimens heavily on the medium Formula Yeast Extract 10 0 g Peptone 8 5 g Dextrose 30 0 g Nitrofuran derivative 0 5 g Agar 13 0 g pH 6 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Czapek Dox Agar Nissui Code an 05705 Directions Suspend 53 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes and cool down about 50 C Distribute about 20 mL of the medium into Petri dishes contained the test specimen in advance mix and incubate at 30 C or 22 C for about 7 days Powder Determinations The fungi grown in the deep layer possess flat folded waves and gradually produce color Those grown on the surface possess folded waves on the surface of the colonies and produce the specific color of the fungi with the incubation Remarks The medium is used for the determination 100 g Dry RT 3 years and cultivation of fungi and is recommended especially for the cultivation of Aspergillus Formula Components Dextrose 36 0 g Sodium Nitrate 2 0 g Dipotassium Phosphate 1 0 g Magnesium Sulfate 0 5g Potassium Chloride 0 5g Ferrous Sulfate 0 01 g Agar 13 0 g pH 6 0
42. sample with the following solution prepared by 1 15 M phosphate buffered solution pH 7 2 containing 1 g of polysorbate 80 1 g of L cysteine hydrochloride and 1 g of agar per liter by autoclaving at 110 C for 15 minutes Remarks The medium was devised for the differentiation of Bacteroides Colistin neomycin and brilliant green have no influence on the growth of Bacteroides but inhibit Fusobacterium and other bacteria Asporogenic gram negative bacilli taken from the clinical specimens are mainly Bacteroides or Fusobacterium The Powder 100 g 8 9 Dry RT 3 years medium also permits the selective growth of Bacteroides from the clinical specimens Formula Peptone 20 0 g Soya Peptone 1 5 g Digested Serum 6 75 g Liver Extract 0 6 g Meat Extract 6 15 g Yeast Extract 10 0 g Hemin 0 003 g Dextrose 3 0 g Sodium Chloride 3 0 g Potassium Dihydrogen Phosphate 2 5 g Soluble Starch 5 0 g L Cysteine Hydrochloride 0 3 g Sodium Thioglycollate 0 3 g Colistin 1 000 000 units Neomycin 0 2 g Brilliant Green 0 001 g Agar 14 7 g pH 7 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD FM Agar Modified Nissui For differentiation and isolation of Fusobacterium Code 05441 Directions Suspend 74 0 g of the dehydrated medium in 1 000 mL of distilled water and heat to dissolve the medium Do not sterilize by autoclaving Distribute the medium into Petri dishes Determination The medium is desig
43. the purpose to observe chlamydospores After incubation examine whether chlamydospores or pseudohypha are present therein The tip of hypha of chlamydospores generally shows an oval form of deep dark color and pseudohypha also shows specific forms respectively 100 g Dry RT 3 years Remarks As the medium has an effective facilitation ability to form pseudohypha it is used for identification of fungi such as Candida albicans that have the ability to form chlamydospores and pseudohypha Formula Corn Meal Percolated Powder 208 Agar 15 0 g pH 6 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD AC Broth Base Nissui For the test of Enterococcus Uninoculated Code an 05680 Powder 100 g Dry RT 3 years Directions suspected colony on the isolation culture Suspend 50 5 g of the dehydrated medium and inoculate to incubate at 45 C for 48 together with 0 25 g of Sodium azide in hours On the broth all gram negative and positive bacteria except Enterococcus are inhibited Consequently Enterococcus is confirmed when any growth turbidity is visually 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute 10 mL of medium into the middle size of test tubes Sterilize by autoclaving at 121 C for 15 minutes observed In case of sample volume is bigger than 10 Formula mL two or three fold concentration broth is prepared according to the volume of Pepton
44. the volume to 1 000 mL Don t heat over 37 C to dissolve Add a proper amount of sodium bicarbonate pH will be 7 1 7 4 at 37 C in an atmosphere of 5 CO in air by adding 1 3 2 2 g sodium bicarbonate Dissolve completely and immediately sterilize by filtration To avoid excessively raising the pH level of the medium it is advantageous to reduce the pH of the medium by gassing with CO before filtration Aseptically add the desire amount of serum Summary Medium 199 Nissui is a powdered medium prepared according to the synthetic medium published by Morgan Morton and Parker in 1950 and contains Earle s BSS This medium is one of the earliest reported synthetic media and was first developed to maintain chick embryo tissues It contains various kinds of amino acids vitamins nucleic acid precursors and others This medium cannot increase cells but is utilized to maintain cells in virus studies and to produce poliovaccine With the addition of serum it is a superior medium for primary cultures Storage and Expiration Close tightly and store at 2 5 C in a dark dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling w
45. will be inhibited and they develop only white colonies even are they grow Solid 100 Plates 15 2 Interpretations Count all blue blue green color colonies grown on the surface Storage Keep dry at 4 10 C Do not freeze Formula Peptone 15 0 g Yeast Extract 5 0 g Sodium Pyruvate 1 0 g Sodium Chloride 5 0 g Disodium Phosphate 2 0 g Potassium nitrate 1 0 g Sodium Lauryl Sulfate 0 15 g S bromo 4 chloro 3 indolyl B 0 15 g D galactopyranoside X GAL Agar 15 0 g pH 7 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui Standard Method Agar SMA Simple and Easy Stamp Medium for Food Hygiene test Total Viable Bacterial Count Code ne 30 Plates 06051 06050 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing This Food Stamp is designed to measure and detect the degree of contamination of the specimen Incubate at 35 37 C for 24 48 hours Solid 100 Plates 15 3 Interpretations Count all colonies grown on the surface Storage
46. 0 g Sodium Chloride 5 0 g Deoxyribonucleic Acid 2 0 g Toluidine Blue O 0 1 g Agar 15 0 g pH 7 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD SC Agar Nissui Code 05111 Directions Suspend 24 2 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 3 mL of the medium into small test tube and sterilize by autoclaving at 121 C for 15 minutes Solidify in a full slanted position Take the bacteria to be tested with a straight wire and smear on the slant Incubate at 37 C for 24 hours Powder Remarks The medium is used for an identification test for Enteric bacteria providing a confirmation of consumption of citrate Positive bacteria change the color of medium into an intense blue with a formation of colonies 100 g Dry RT 3 years Formula Components Sodium Chloride 5 0 g Magnesium Sulfate 0 2 g Monoammonium Phosphate os Dipotassium Phosphate 1 0 g Sodium Citrate 2 0 g Brom Thymol Blue 0 024 g Agar 15 0 g pH 6 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD VP MR Medium Nissui Dextrose Phosphates Peptone Water Voges Proskauer Methyl Red Medium Code 05107 Directions Suspend 17 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 2 mL of the medium into small test tube and sterilize by autoclaving at 121 C for 15 mi
47. 14 N ID Test SP 18 Nissui SP Broth 06638 N ID Test SP 18 Nissui SP Reagent aaaaaaaaaananannnar ra 6 5 Haemophilus Neisseria 06635 ID Test HN 20 Rapid Nissui 06640 ID Test gt HN 20 Rapid Nissui HN Reagent 7 1 i C NISSUI PHARMACEUTICAL CO LTD Index Dehydrate Media Anaerobes 05420 GAM Agar NISSU aaaaaaaaanaaaaannnnnnnnunnnnnnnnnunnnnnnnnnnnnnnnnunnnunannnunaa 8 1 05426 GAM Agar Modified NiSSUI aaaaaaaaaaaaaaannnnnnnnnnnnnunnnnnnnnnnunna 8 2 05433 GAM Broth Modified NiISSUI aaaaavavnanununn ann an vnavnanannnnnnnnnnnnnnnnnn 8 3 05422 GAM Broth NISSUI aaaaaaaaaaaaanaaannnnnnnnunnnnnunnnnnnnnnnnnnnnnnnnnunannnnnna 8 4 05460 GAM Semisolid without Dextrose NISSUI a2uaaaaaaannnunnnnivvra 8 5 05424 GAM Semisolid NiISSUI aaaaaaaaaaaaaaannnunnnnunnnnnnnnnnunnnnnnnnnnnnnnnna 8 6 05404 CW Agar Base without KM NiSSUI aaaaaannnaaanaaannnnnannnnnnunnnna 8 7 05405 CW Agar Base with KM NISSUI aaaaanaaaaaaanaaanannnnnnnnnnnnnnnnnnnnna 8 8 05440 Bacteroides Agar NiISSUI aaaaaaaaaanaaanannnnnnnnnnnnnnunnnnnnnnnnnnnnnnnna 8 9 05441 FM Agar Modified NISSUI aaaaanaaaaaanaaannnnnnnnnnnnnnnnnnnnnnnnnnna 8 10 05450 GAM Agar with GM NISSUI aaaaaaaaaaannnnnnnnnnunnnnnnnnnnnnnannnnnnna 8 11 05430 BL Agar NISSUI aaaaaaaaaaanaaannnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnnnnnna 8 12 05409 Clostridia
48. 15 minutes and cool immediately to a deep agar Take bacteria to be tested with a straight wire and stab into the butt of the medium Incubate at 37 C for 24 hours Powder Remarks The medium as a differentiation medium permits the tests of motility as well as productions of indole and hydrogen sulfide of the bacteria Observation of indole production When an indole reagent is laid over positive bacteria change the color of reagent into red though negative one does not 100 g Dry RT 3 years Observation of hydrogen sulfide Positive bacteria change the color of medium into black Observation of motility Positive bacteria grow to get turbid whole deep agar Negative bacteria grow only along with the stab portion Formula Meat Extract 3 0 g Peptone 28 0 g Sodium Thiosulfate 0 025 g Ferric Ammonium Citrate 1 0g Agar 3 0g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD DNA Agar Nissui Deoxyribonucleic Acid Agar for detection of DNase Code an 05148 Directions Suspend 42 1 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute about 20 mL of the medium into Petri dishes and dry out the surface of the medium before use Determinations For the detection of DNase production inoculate the freshly cultured bacteria by streaking on the plate and incubate at 37 C fo
49. 22 26 hours E coli one of coliform organisms forms colonies of metallic luster with a black center while Enterobacter aerogenes forms larger colonies with a dark center Salmonella and Shigella form colorless transparent and small colonies Remarks The medium is used for the differentiation of coliform organisms in foods and drinks 300 g Dry RT 3 years The growth of gram positive organisms is inhibited by the dyes Formula Peptone 10 0 g Lactose 10 0 g Dipotassium Phosphate 2 0 g Eosin Y 0 4 g Methylene Blue 0 065 g Agar 15 0 g pH 6 8 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD X GAL Agar Nissui For the test of Coliform group Code sane 05631 Directions Suspend 44 3 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute 20 mL amounts of medium into Petri dish For the mixing and dilution culture of liquid specimen the medium may be used in pour plate technique Granule Remarks The medium is used for the test for coliform group in foods and drinks Coliform group develop a blue to blue green colony after incubation at 35 37 C for 20 2 hours Growths of almost all other bacteria are inhibited and they develop only white colony even if they grow X GAL colorimetric enzyme substrate in the medium is decomposed to bring out 300 g Dry RT
50. 3 g Sodium Thioglycollate 0 3 g Agar 1 5 g pH 7 1 8 6 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD CW Agar Base without Kanamycin Nissui Clostridium welchii Agar base without Kanamycin For examination of heated materials Code 05404 Directions Suspend 60 0 g of the dehydrated medium in 900 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Maintain the sterilized medium at about 50 C add 100 mL of the egg yolk saline suspension or defibrinated blood Mix well Powder and distribute about 20 ml into Petri dishes To prepare a 100 mL of egg yolk solution mix about 20 g of egg yolk with 80 mL sterilized physiological saline solution Determinations After drying the surface of the plate smear the heated material and incubate for 15 20 hours under anaerobic condition Clostridium welchii perfringens forms yellowish white round convex colonies with a glistering surface and surrounded by a pearly ray zone opacity of the medium 100 g 8 7 Dry RT 3 years On the medium added with defibrinated blood instead of egg yolk Clostridium welchii perfringens forms colonies of the same type with a glistering surface and a hemolytic ring When the colonies are exposed to the air in an incubator they will turn green Formula In 60 0 g L Heart Extract 5 0 g Proteose Peptone 10 0 g Peptone 10 0 g Sodium Chl
51. 80 1 0 g Agar 15 0 g pH 6 9 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD B Inoculum Broth Nissui Code an 05803 Powder 100 g Dry RT 3 years Directions Formula Buspend 34 7 g ai the cenyelate medium In 34 7 g L in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into Yeast Extract 8 5 g the test tube sterilized by autoclaving at Peptone 8 5 g 121 C for 10 minutes Dextrose 11 0 g Remarks Potassium Dihydrogen 36 The medium is used for the preparation of Phosphate UE inoculation for L leichmannii for B g quantitative analysis Tomato Juice Powder 3 7 g Polysorbate 80 1 0 g pH 6 9 Back to the Index 13 4 C NISSUI PHARMACEUTICAL CO LTD B Assay Medium set Nissui 6 x 2mL 10 Polysorbate 80 05819 Directions Suspend 8 3 g of the dehydrated medium in about 80 90 mL of distilled water mix well and heat to dissolve the medium and cool down the solution After adjusting the pH to 6 2 0 1 add 2 0 mL of Polysorbate 80 and add distilled water to make total volume 100 mL Distribute into the test tube and double the total volume with the distilled water after adding the sample Sterilize by autoclaving at 121 C for 5 minutes Inoculate the strain for culture at 37 C for 16 24 hours Incubate 72 hours for the purpose of acidimetry Code Remarks Strain to be used is Lactobacillus leichmannii ATCC7830 available also from Nissui
52. ACEUTICAL CO LTD BTB Lactose Agar Nissul Code an 05042 Directions Suspend 40 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL amounts into the Petri Dish after being sterilized by autoclaving at 121 C for 15 minutes Incubate at 37 C for about 18 24 hours Since the medium has a very weak selectivity small colonies of Staphylococcus and or gram positive Cocci may grow Powder Remarks Organisms non fermenting lactose such as Salmonella and Shigella form colorless semitransparent colonies while organisms fermenting lactose such as coliform form yellow colonies 300 g Dry RT 3 years Formula Components Meat Extract 5 0 g Peptone 10 0 g Lactose 10 0 g Brom Thymol Blue 0 08 g Agar 15 0 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD MacConkey Agar Nissui 05037 Directions Suspend 50 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes and distribute about 20 mL into Petri dishes aseptically and dry the surface of the medium before use Granule Determinations Incubate at 30 35 C for 18 72 hours Pathogenic bacteria form semitransparent colonies Colonies of E coli assume an intensely redbrick color and produce light pink or redbrick sediments around Remarks Features
53. ARMACEUTICAL CO LTD Compact Dry Nissui X SA Simple and Easy Dry Medium for Microbial Count Staphylococcus aureus Link to User s Manual English Traditional Chinese Simplified Chinese Code sen 06729 06730 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 35 2 C for 24 2 hours Interpretations Staphylococcus aureus generates light blue blue colonies Some bacteria other than Staphylococcus aureus may grow and generate white colonies Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui SL Simple and Easy Dr
54. CD Agar SCD esseeeeeees 17 2 06787 Clean Stamp Nissui SCD Agar with Lecithin polysorbate 80 SCDLP 06786 Clean Stamp Nissui SCD Agar with Lecithin polysorbate 80 SCDLP 17 3 06783 Clean Stamp Nissui Mannitol Salt Agar with Egg Yolk MSEY 06782 Clean Stamp Nissui Mannitol Salt Agar with Egg Yolk MSEY 17 4 06791 Clean Stamp 25 Nissui SCD Agar SCD 06790 Clean Stamp 25 Nissui SCD Agar SCD 00008 17 5 06789 Clean Stamp Nissui Cetrimide Kanamycin Sulfate Nalidixic Acid Agar PSEU aaaaaaaaaaaaaaa nanannnnnnnnnnnnunanna 17 6 06793 Clean Stamp 25 Nissui SCDLP Agar SCDLP 06792 Clean Stamp 25 Nissui SCDLP Agar SCDLP 17 7 06795 Clean Stamp 25 Nissui Sabouraud Agar with Chloramphenicol CPSB 06794 Clean Stamp 25 Nissui Sabouraud Agar with Chloramphenicol CPSB 17 8 vii C NISSUI PHARMACEUTICAL CO LTD Index Food Allergen Detection Kit 08630 08631 08632 08633 08634 08615 08616 08617 08618 08619 08606 08621 08622 08623 FASTKIT SLIM EGG Nissui FASTKIT SLIM MILK Nissui FASTKIT SLIM WHEAT Nissui FASTKIT SLIM BUCK WHEAT Nissui FASTKIT SLIM PEANUT Nissui 222aaaaaanaaavanr nsnnnannnnunnnnn 18 1 FASTKIT ELISA ver ll EGG Nissui FASTKIT ELISA ver ll MILK Nissui FASTKIT ELISA ver ll WHEAT Nissui FASTKIT ELISA ver ll BUCK W
55. Count Agar NISSUI aaaaaaanaaaaann nannnnnnnnnnnnnananum 8 13 Bacillus cereus 05282 NGKG Agar Base NISSUI aaaaaaaaaaanaaanannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna 9 1 Viable bacteria 05530 Sensitivity Disk Agar N NiISSUI aaaaanaaaaaannannnannnnnnnnnnnnnunnnnna 10 1 05533 Mueller Hinton Agar N NiSSUI 2uaaaaanaaaaaaan nannnnnanannnnnnnnnnna 10 2 05534 Sensitivity Test Broth NiSSUI aaauaaaaaaaannanannnnnnnnnnnnnnnunnnnnna 10 3 05503 Heart Infusion Agar NiSSUI aaaaaaanaaaaaaan nnunnnnnnunnnnnnnnnnnnnna 10 4 05505 Heart Infusion Broth NISSUI aaaaaanaaaaaaan nnnunnnnnnnnnnnnnnnnnnnna 10 5 05507 Brain Heart Infusion Agar NISSUI aaaaaaaaaanaaaaann nannnnnnnnnna 10 6 05509 Brain Heart Infusion Broth NiSSUI aauaaaaaaaaanaaanannnnnnnnnnna 10 7 05514 Nutrient Agar NISSUI aaaaaaanaaaaaan nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna 10 8 05511 Nutrient Broth N SSU aaaaaaaaaaaaaaaanannnnnnnunnnnnnnnnnnnnnunnunnnnnnna 10 9 05516 Trypto Soya Agar SCD Agar NISSU aaaaaaaaannaannnannnnna 10 10 05630 Trypto Soya Broth SCD Broth NiSSUI 00008 10 11 05528 Buffered Sodium Chloride Peptone Solution pH7 0 Nissui 10 12 05522 Dorset Egg Medium NISSUI aaauaaaaannnnnannnnnnnnnnnnnnnnnnnuna 10 13 05527 CLED Agar NISSUI aaaaaaaaaaaanaannnnnnnnnnnunnnnunnnnunnnnnnnnnnunannnna 10 14 05601 TGC Medium Fluid NiSSUI aaaaaaaaaaanananannnunnnnnn
56. D FASTKIT ELISA ver Il Nissui oe Huskers store _ Shelf Life B 08615 08616 08617 08618 08619 08606 a WHEAT BUCK WHEAT PEANUT SOYBEAN Introduction Amendments to Food Sanitation Law in Japan have rendered it mandatory to label 5 specific raw food materials Eggs Milk Wheat Buckwheat and Peanuts identified to have a high possibility of triggering food allergies when foods contain more than a predetermined amount of g mL or ug g of the specified raw ingredients as of April 2002 In addition to clearly labeling the fore mentioned food products screening of such products is becoming increasingly important in order to confirm the amount of proteins into contained in the raw ingredient or confirm the existence by a result of contamination due to the mixing of these raw materials with other foodstuffs and contamination on production lines FASTKIT ELISA Version II are the test kits for detecting proteins in foodstuffs by using enzyme linked immunosorbent assay ELISA Product Features The FASTKIT ELISA Version II are able to accurately measure the amount of proteins in foods 18 2 The FASTKIT ELISA Version II can simultaneously identify the presence of multiple proteins The FASTKIT ELISA Version II are widely applicable to samples ranging from raw ingredients to processed foods Formula A Antibody immobilized plate 96 Wells x 1 B Standard solution 50 ng mL 1 8 mL x 1 C Dilution buf
57. Extract Powder 10 0 g Peptone 10 0 g Sodium Chloride 5 0 g Agar 15 0 g pH 7 4 0 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Heart Infusion Broth Nissui Code an 05505 Directions Suspend 25 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into adequate containers according to the purpose and sterilize by autoclaving at 121 C for 15 minutes Powder Remarks Since the medium has an excellent ability to support the growth of many kinds of bacteria it is suitable for the cultivation and preservation of many fastidious bacteria The medium does not affect the biological nature of bacteria and it is also suitable for precise biological tests The medium may be employed in the routine cultivation and for various other purposes because the medium does not contain any carbohydrate such as dextrose 100 g Dry RT 3 years Formula Heart Extract Powder 10 0 g Peptone 10 0 g Sodium Chloride 5 0 g pH 7 2 0 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Brain Heart Infusion Agar Nissui Code an 05507 Directions Suspend 50 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Use as plates or slants Powder Remarks Since the medium has an excellent ability to support the growth of many kinds of bacter
58. HEAT Nissui FASTKIT ELISA ver ll PEANUT Nissui FASTKIT ELISA ver ll SOYBEAN NiSSUI 2222aaaaaaaaaaannna 18 2 FA test ElA Crustacea Nissui FA test Extraction Solution NiISSUI n2v2v2 2 222vvvvvv annvvvvsr run 18 3 FA test Immunochromato Crustacea Nissui 18 4 Tissue Culture Medium 05900 08160 05901 05902 05904 05913 08190 08192 05908 05915 05919 05963 05911 05918 05971 Eagle s MEM Nissui 1 Eagle s MEM Nissui Q aaaunavnaavn nnanrnnnnnnnnnnnnnannnannannannannann 19 1 Eagle s MEM Nissui aauaauaannav nan nansnannannnnnnnnnnnnnnannnnnnum 19 2 Eagle s MEM Nissui aaaanaaaananaaa nannaannnnnnnannannnannannnnnann 19 3 Eagle s MEM Amino Acids and Vitamins Medium Nissui 19 4 Dulbecco s PBS Nissui Dulbecco s PBS Nissui Dulbecco s PBS NiSSUI uaaaaaaaa vanan nnnnnnnunnnnnunnnnnnnnan 19 5 Glutamine NiSSUI aaaaaaaaaaaaaaaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna 19 6 Dulbecco s Modified Eagle Medium Nissui Q 19 7 Dulbecco s Modified Eagle Medium Nissui 19 8 SFM 101 NISSU uaaaanaaaaananan nnnunnnunnnnnnnnannnannnnnnnanannnnnnanaa 19 9 RPMI 1640 Medium Nissui CD wees 19 10 RPMI 1640 Medium Nissui Q aaavaaavvavvrsvnnrsnnninan 19 11 ES Medium N SSU citi 19 12 viii CO NISSUI PHARMACEUTICAL CO LTD
59. O LTD Food Stamp Nissui X SA Agar X SA Simple and Easy Stamp Medium for Food Hygiene test Staphylococcus aureus Code nt 30 Plates 06757 06756 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 22 24 hours Interpretations Staphylococcus aureus forms blue light blue lenticular wet grazed colonies and Coagulase negative staphylococcus CNS Solid 100 Plates 15 9 forms small white or light blue colonies Small light blue matte flat colonies are not Staphylococcus aureus Storage Keep dry at 4 10 C Do not freeze Formula Peptone 14 0 g Meat Extract 3 0 g Lithium Chloride 5 0 g Mannitol 10 0 g Agar 14 0 g Selective Agent 0 7 g Chromogenic Substrate 0 2 g pH 7 4 0 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui Sabouraud Agar SABO Simple and Easy Stamp Medium for Food Hygiene test Bacillus cereus Code nee 30 Plates 06064 06063 Directions Food Stamp is a prepared agar
60. Powder 05901 Directions Dissolve 9 4 g of the medium in distilled water and adjust the volume to 1 000 mL Sterilize by autoclaving at 121 centigrade for 15 minutes Cool the sterilized medium to room temperature and add a proper amount of sterile 10 sodium bicarbonate solution pH will be 7 1 7 4 at 37 centigrade in an atmosphere of 5 CO in air by adding 12 5 22 0 mL of sodium bicarbonate solution This mixture should be stopper tightly and stored in a cool dark place At the time of use aseptically add 0 292 g of L glutamine sterilized and the desired amount of serum Summary Eagle s MEM Nissui 2 is a powdered medium prepared according to prescription published by Harry Eagle in 1959 Up to now such media were not autoclavable because of decomposition or deterioration of the constituents so that sterilization was performed by filtration using a membrane filter Nissui Pharmaceutical Co Ltd has succeeded in preparing an autoclavable powdered medium This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired This medium has a growth promoting effect for cells such as HeLa or L and other cell lines Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life Wh
61. RMACEUTICAL CO LTD Lactobacilli Inoculum Broth Nissui Code an 05801 Powder 100 g Dry RT 3 years Directions Formula ee ice aan in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into Yeast Extract 5 5 g the test tube sterlizeg by autoclaving at Peptone 12 5g 121 C for 10 minutes Dextrose 11 0 g Remarks Potassium Dihydrogen 0 25 The medium is used for the preparation of Phosphate 298 inoculation for Lactobacilli Dipotassium Phosphate 0 25 g Sodium acetate 10 0 g Magnesium Sulfate 0 1 g Manganese Sulfate 5 0 mg Ferrous Sulfate 5 0 mg pH 6 8 Back to the Index 13 2 C NISSU PHARMACEUTICAL CO LTD B42 Culture Agar Nissui Code an 05802 Directions Suspend 49 7 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into the test tube sterilized by autoclaving at 121 C for 10 minutes and use after solidifying in the butt position Stab the medium for culture at 37 C for 18 24 hours and preserve in cool place Subculture 2 3 times a week Powder Remarks The medium is used for the preservation of L leichmannii for B quantitative analysis The medium becomes clouded just after autoclaving due to high temperature and becomes clear when cool down 100 g Dry RT 3 years Formula Yeast Extract 8 5 g Peptone 8 5 g Dextrose 11 0 g a Dihydrogen 2 0 g Tomato Juice Powder 3 7 g Polysorbate
62. RT 06618 NF Broth 100 tests 06631 NF Reagent Cool 1 year Detection Test Solution I and II NIT into certain wells Features of ID Test NF 18 After addition of specific reagents compare the color developed Identification of bacteria is very complicated and there are so with the attached color chart and determine as positive or many biochemical test need to be done ID Test NF 18 is based on negative results Simultaneously an oxidase test shall be the theory of the numerical classification method which could performed and record in the result form receive the combination with 18 kinds of biochemical reactions Only small amount of sample 0 1 mL is needed for each test Interpretations item Also the kit is stable for 1 year at room temperature Refer ID Test NF 18 ANALYTICAL PROFILE for identification Purpose of ID Test NF 18 Formula Identification of Glucose Non fermentative Gram Negative bacilli Kit component 06629 Directions for operation of the ID Test NF Plate 25 plates x 18 wells Sterilized Liquid Paraffin 20 mL x 2 vial kit Result Form 13 sheets x 2 sets Streak samples onto blood agar or other isolation medium After ID Test NF Broth 2 5 mL x 25 tubes incubation of the medium confirm colonies will be tested are Color Chart 1 sheet Gram Negative bacilli and Glucose fermentative The test target bacteria shall be proliferated on enrichment Kit component 06631 medium Nitrite Detection Test EMA vial Fish colonies from enr
63. Remarks The medium is suitable for the enrichment culture of Vibrio parahaemolyticus from foods especially from fish and fish products Powder and for counting the number of bacteria MPN values Furthermore it is suitable for 100 g Dry RT 3 years cultivation of the materials that seem to be deficient in the number of Vibrio parahaemolyticus Features The preservation of the medium after preparation is easy because the medium can be autoclaved Since Polymyxin B a selective antibiotic is unlikely to undergo its inactivation by metabolities of bacteria the medium can be incubated overnight up to 24 hours and is also convenient for routine examination Formula Peptone 10 0 g Yeast Extract 3 0 g Sodium Chloride 20 0 g Polymyxin B 250 000 unit pH 7 4 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Alkaline Peptone Water Nissui Enrichment of V cholerae and V parahaemolyticus Code 05206 Directions Suspend 20 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 10 mL of the medium into middle size of test tube and sterilize by autoclaving at 121 C for 15 minutes The medium is used for an enrichment medium of Vibrio cholerae as well as Vibrio parahaemolyticus They grow mostly on the surface of the medium Powder Remarks Since the medium has a weak selectivity an adequate incubation is at 37 C fo
64. aan nannnanannnunnnnnunnnnnnnnnnnnana 13 3 05803 B12 Inoculum Broth NISSUI aaaaaaaaaaaaaaaaannnnnnnunnnnnnnnnnnananuna 13 4 05819 B12 Assay Medium Set NISSUI aaaaaaananaaannnannnnnnnnnnnnnnnnna 13 5 05815 Pyridoxine Assay Medium NISSUI aaaaaannaaaaunanannnnnnnnnnnnna 13 6 05816 Niacin Assay Medium NiSSUI aa2uunaaaaaaannannnnnnnnnnnnnnnnnunnnna 13 7 05814 Folic Acid Assay Medium NISSUI uaaaaannaaaaaannnnnunnnnnnnunnnnna 13 8 05817 Pantothenate Assay Medium NISSUI aaaaannaaannnnnnnnnnnnra 13 9 05818 Biotin Assay Medium NISSUI aaaaaanaaaaaaanannunnnnnnnnnnnnnnnna 13 10 05835 Powdered Agar NISSUI aaaaaaaanaaaaananannunnnnnnnnnnnnnnnnnnnnnnnna 13 11 Prepared Media Tube Media 05169 _ Nissui Tube Triple Sugar Iron Agar 2aaaaanaaaaanan nunnnnnanna 14 1 05170 Nissui Tube Sulfide Indole Motility Medium 14 2 05171 Nissui Tube Lysine Indole Motility Medium 14 3 Food Stamp 06053 Food Stamp Nissui Desoxycholate Agar DESO 06052 Food Stamp Nissui Desoxycholate Agar DESO 15 1 06764 Food Stamp Nissui X GAL Agar XGAL 06763 Food Stamp Nissui X GAL Agar XGAL aaaaaaanaaaaan ananna 15 2 06051 Food Stamp Nissui Standard Method Agar SMA 06050 Food Stamp Nissui Standard Method Agar SMA 15 3 06776 Food Stamp Nissui XM G Agar XM G 06775 Food Stamp Nissui XM G Agar XM G aaaaaaaanaaaa
65. adding the sample Sterilize by autoclaving pH 7 1 0 1 at 121 C for 5 minutes Inoculate the strain for culture at 30 C for 16 24 hours Back to the Index Incubate 72 hours for the purpose of acidimetry Remarks Strain to be used is Streptococcus faecalis R ATCC 8043 available also from Nissui Range of response 0 5 ng 5 ng tube 10 mL 13 8 o gt NISSUI PHARMACEUTICAL CO LTD Pantothenate Assay Medium Nissui 05817 Powder Directions 0 01 pg 0 1 ug tube 5 mL 0 01 ug 0 05 pg tube 2 mL 3 years Code an Suspend 7 7 g of the dehydrated medium in about 80 90 mL of distilled water mix well and heat to dissolve the medium and cool Storage down the solution After adjusting the pH to Cap tightly and keep at cool place 2 7 1 add distilled water to make the total 10 C volume 100 mL Distribute into the test tube and double the Formula total volume with the distilled water after As directed in U S P XV adding the sample Sterilize by autoclaving pH 7 1 0 1 at 121 C for 5 minutes Inoculate the strain for culture at 37 C for 16 24 hours Back to the Index Incubate 72 hours for the purpose of acidimetry Remarks Strain to be used is Lactobacillus arabinosus strain 17 5 ATCC 8014 available also from Nissui Range of response 0 02 pg 0 2 ug tube 10 mL 13 9 o gt NISSUI PHARMACEUTICAL CO LTD Biotin Assay Medium Nissui 05818 Powder Directions 0 1 n
66. annanannnna 15 4 06055 Food Stamp Nissui TCBS Agar TCBS uaaaaaaaaaaaaaaaaaannnnr 15 5 Index Prepared Media Food Stamp 06054 06057 06056 06751 06750 06753 06752 06757 06756 06064 06063 06755 06754 06231 Food Stamp Nissui TCBS Agar TCBS aa annan aanaaaaannna Food Stamp Nissui TGSE Agar TGSE Food Stamp Nissui TGSE Agar TGSE aaa aaaaaaaaaaania Food Stamp Nissui MLCB Agar MLCB Food Stamp Nissui MLCB Agar MLCB Food Stamp Nissui Cereus Agar CERE Food Stamp Nissui Cereus Agar CERE Food Stamp Nissui X SA Agar X SA Food Stamp Nissui X SA Agar X SA aaaaaaaaaaaannn nnnnnnna Food Stamp Nissui Sabouraud Agar SABO Food Stamp Nissui Sabouraud Agar SABO Food Stamp Nissui Potato dextrose Agar with Chloramphenicol PDA Food Stamp Nissui Potato dextrose Agar with Chloramphenicol PDA Food Plate X NiISSUI aaaaaaannn annuanaannnnnnnnnnnnnnnnnnnnunnnnnnnnnnnnnna Compact Dry Link to Product s Info English Traditional or Simplified Chinese 06740 06741 06744 06745 06742 06743 06746 06747 06748 06749 06729 06730 06732 06733 Compact Dry Nissui TC Compact Dry Nissui TC aaaaaaaanaaanannnnnnnnnnnnnnnnnnnnnnnnnnnunnnna Compact Dry Nissui CF Compact Dry Nissui CF aaaaaaazaanaaaaanannnnnnn
67. at to dissolve the medium Sterilized by autoclaving at 115 C for 20 minutes After cooling to 50 C add defibrinated horse blood in the ratio of 5 percent and mix well Distribute into Petri dishes and solidify Dry the surface before use Determinations Bifidobacterium generally forms smooth and brown or dark brown colonies Some enteric bacteria form red colonies For the details see the World of Intestinal Bacteria written by Dr Tomotari Mitsuoka Remarks The medium permits the growth of anaerobic bacteria especially lactobacilli and enables to differentiate various bacteria easily because they will each form characteristic colonies Powder 300 g Dry RT 3 years Formula Meat Extract 2 4 g Proteose Peptone 10 0 g Peptone 5 0 g Soya Peptone 3 0 g Yeast Extract 5 0 g Liver Extract 3 2 g Dextrose 10 0 g Soluble Starch 0 5 g Monopotassium Phosphate 1 0 g Potassium Dihydrogen Phosphate 1 0 g Magnesium Sulfate 0 2 g Ferrous Sulfate 0 01 g Sodium Chloride 0 01 g Manganese Sulfate 0 007 g Bubble Absorbing Agent Silicon 0 2 g Polysorbate 80 1 0 g L Cysteine Hydrochloride 0 5 g Agar 15 0 g pH 7 2 8 12 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Clostridia Count Agar Nissui Code 05409 Directions Suspend 70 3 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes and keep t
68. ater after use and then disposed as industrial waste according to the law or regulations related to dispose Package 100 g Storage Formula L Arginine Hydrochloride L Histidine Hydrochloride H O L Lysine Hydrochloride L Tryptophan L Phenylalanine L Methionine L Serine L Threonine L Leucine L Isoleucine L Valine L Glutamic Acid L Aspartic Acid L Alanine L Proline L Hydroxyproline Glycine L Glutamine L Cystine L Tyrosine L Cystelne Hydrochloride H O p Aminobenzoic Acid Biotin Calcium Pantothenate Folic Acid Inositol Nicotinic acid Nicotinamide Pyridoxal Hydrochloride Pyridoxine Hydrochloride Riboflavin Thiamin Hydrochloride Vitamin A Ascorbic Acid a Tocopherol Disodium Phosphate Calciferol Menadione Choline Bitartrate D 2 Deoxyribose Adenine Guanine Hydrochloride Hypoxanthine Thymine Uracil Xanthin Adenylic Acid D Ribose Polysorbate 80 Cholesterol Glutathione Sodium Acetate Adenosine Triphosphate Disodium Salt Glucose Sodium Chloride Potassium Chloride Calcium Chloride Magnesium Sulfate Sodium Dihydrogen Phosphate Ferric Nitrate 9H 0 Phenol Red Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Shelf Life 1 Year n 9 5 g L 70 0 mg 21 9 mg 70 0 mg 10 0 mg 25 0 mg 15 0 mg 25 0 mg 30 0 mg 60 0 mg 20 0 mg 25 0 mg 66 8 mg 30 0 mg 25 0 mg 40 0 mg 10 0 mg 50 0 mg 100 0 mg 20 0 mg 40 0 mg 0 11 mg 0 05 mg 0 01 mg 0 01 mg 0 01 mg
69. be contaminated with a relatively small number of the causative organisms The first step of isolation for the specimen such as river water is the pre enrichment in Powder 240 g 4 4 Dry RT 3 years Alkaline Peptone Water at 35 C for 6 hours Next the culture is inoculated in Monsur Peptone Water and incubate at 35 C for one night Then the enrichment culture is placed onto PMT Agar El Tor cholera vibrio grown on the medium can clearly agglutinate the antiserum of V cholerae When the medium is used for the clinical specimens such as diarrheal feces it is desirable to use it in combination either with Vibrio Agar or with TCBS Agar simultaneously Formula Peptone 10 0 g Meat Extract 5 0 g Sodium Chloride 10 0 g D Mannose 20 0 g Sodium Lauryl Sulfate 0 2 g Polymyxin B 180 000 units Brom Thymol Blue 0 04 g Cresol Red 0 04 g Agar 14 7 g pH 8 4 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD TCBS Agar Nissui Isolation of V cholerae and V parahaemolyticus Form Code 05204 Directions Suspend 86 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute immediately about 20 mL of the medium into Petri dishes Do not autoclave Dry the surface of the medium before use Determinations Inoculate the specimens directly or after enrichment culture and incubate at 37 C for 20 24 hours Saccharose fermentative bacteria such a
70. ce Shelf life of the product is one 1 year after manufacturing Precautions Use a low protein adsorption filter to prevent the protein Insulin and Transferrin from being adsorbed on the filter Store reconstituted Supplement A below 20 C if it its not used at once However avoid repeated freezing and thawing Prepared medium should be used within two months Summary SFM 101 is a serum free medium developed by Nissui Pharmaceutical Co Ltd and is highly effective for both cell growth and the production of antibodies using mouse hybridomas SFM eliminated the problems caused by differences in batches of fetal calf serum etc And because it contains a considerably lower concentration of protein than media with serum it allows easier collection and purification of cell products monoclonal antibodies and physiologically active substances 19 9 1 Year Formula Sodium Chloride 6 000 0 mg Potassium Chloride 400 0 mg Sodium Dihydrogen Phosphate 2H 0 75 0 mg Disodium Phosphate 2H 0 502 0 mg Calcium Chloride 100 0 mg Calcium Nitrate 4H 0 50 4 mg Magnesium Sulfate 71 0 mg Sodium Selenite 0 002 mg L Arginine 100 0 mg L Arginine Hydrochloride 78 0 mg L Asparagine H20 43 4 mg L Asparatic Acid 10 0 mg L Cysteine Hydrochloride H20 15 7 mg L Cysteine Dihydrochloride 32 5 mg L Glutamic Acid 10 0 mg L Glutamine 600 0 mg Glycine 10 0 mg L Histidine 7 5 mg L Histidine Hydrochloride H20 21 0 mg L Hydroxyproline 10 0 m
71. contains ONPG o0 nitrophenyl B D galactopyranoside peptone and sodium phosphate Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Cytochrome Oxidase Test Strip Nissui 05180 Piece Directions Put a piece into a Petri dish add several drops of distilled water to moisten the piece wholly and immediately smear organisms to be tested colony or slant culture on the strip using a platinum loop or a glass rod Note When a nichrome wire loop is used use as fresh a one as possible covering it with absorbent cotton As the water should not contain iron ion use distilled water or pure water passed through ion exchange resin Code sane Determinations Cytochrome oxidase positive bacteria turn the smeared area into deep blue within one minute but the area remains unchanged in case of negative bacteria 15 Pieces Remarks This test strip was originally devised to examine the presence of cytochrome oxidase bacteria simply and accurately Accordingly the strip is very suitable for the examination of biological property for Aeromonas Pseudomonas Vibrio etc and for the differentiation of these bacteria from Enterobacteriaceae Storage Store in a dark and cool place If not stored properly the strip will turn blue before use Such a strip should not be used Formula This product is a simplified Kovacs oxidase test Back to the Index C NISSUI PHARMACEUTICAL CO LTD ID Test EB 20
72. culture Do not leave any positive specimen in the medium because they may be killed by a group of dextrose fermentation bacteria that grow utilizing dextrose in the medium 300 g Dry RT 3 years It is also possible to culture Trichomonas vaginalis by adding serum 10 of the medium Formula Components Peptone 17 0 g Soya Peptone 3 0 g Dextrose 6 0 g Sodium Chloride 2 5g Agar 0 7 g Sodium Thioglycollate 0 5g L Cystine 0 25g Sodium sulfite 0 1g pH 7 1 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Dextrose Peptone Broth Nissui Aseptic Test for Fungi Mold and Yeast Code 05602 Powder 300 g Dry RT 3 years Directions Formula puspend 28 5 g oi the renyarated medium in 28 5 g L in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into Dextrose 20 0 g appropriate containers and SIETZE by Yeast Extract 2 0 g autoclaving at 121 C for 20 minutes Incubate at 20 25 C for at least 10 days Magnesium Sulfate 0 5 g Peptone 5 0 g Remarks If the growth of fungi were not distinctive subculture into the fresh medium for continued incubation at 20 25 C for more pH 5 7 than 10 days for final evaluation Potassium Dihydrogen Phosphate 10E Back to the Index 10 18 C NISSUI PHARMACEUTICAL CO LTD Standard Method Agar Nissui unt For viable count Code 05618 Directions Suspend 23 5 g of the dehydrated medium
73. d disinfectants absorbed into medium more reliable results can be obtained Incubate at 30 35 C for 2 5 days Interpretations Count all colonies grown on the surface Remarks Clean Stamp 25 SCDLP is made and prepared of Code Solidified 150 Plates 17 7 05516 Trypto Soya Agar Nissui plus Lecithin and Polysorbate 80 according to its directions and aseptically distributed and solidified in the special Petri dishes having an inside diameter of 58 mm outside diameter of 73 mm and height of 18 mm One clean film bag contains 3 Clean Stamp 25 s connected in series Storage Do keep at 4 10 C Do not freeze Formula Peptone 15 0 g Soya Peptone 5 0 g Sodium Chloride 5 0 g Lecithin 1 0 g Polysorbate 80 7 0 g Agar 15 0 g pH 7 2 0 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Clean Stamp 25 Nissui Sabouraud Agar with Chloramphenicol CPSB Microbiological Test for Environment Fungus Code m 06795 30 Plates R Solidified 06794 150 Plates Directions Nissui plus Chloramphenicol according to Clean Stamp 25 CPSB is a prepared agar its directions and aseptically distributed medium for Stamp method on which agar and solidified in the special Petri dishes stands up slightly above the rim of special HOVE a inside diameter of 58 me Petri dish of 25 cm2 Take off the cap of outside diameter of 73 mm and height of Clean Stamp 25 CPSB and gently press the 18 mm One clean film bag c
74. e 20 0 g sample Yeast Extract 5 0 g For preliminary confirmation test of Dextrose 5 0 g Enterococcus in water or foodstuffs Sodium Citrate 10 0 g incubate at 37 C for 48 hours For Sodium Chloride 5 0 g confirmation test incubate at 45 C for 48 Disodium Phosphate 4 0 g hours for assessment of MPN Potassium Dihydrogen Remarks Phosphate 5 For an identification of Enterococcus obtained from clinical samples fish a Back to the Index 12 3 o gt NISSUI PHARMACEUTICAL CO LTD Candida GE Agar Nissui 05703 Directions Suspend 62 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL amounts into Petri dishes Do not autoclave Determinations After drying the surface inoculate the slime in oral or vaginal secreta with a sterilized swab and spread on the surface of the medium Incubate at 35 37 C for 2 3 days Candida forms specific colonies that are round or oval in shape with a diameter of 3 5 mm and are wet and opaque and have specific odor and luster C albicans forms colonies of creamy or off white color after 48 72 hours incubation which gradually turn pale brownish C krusei forms flat and irregularly shaped colonies with no luster while the other Candida form brown or pale brown colonies Powder 100 g Dry RT 3 years Remarks e Features 1 The medium inhibits the growth of miscellaneous bacteria and
75. e cap again immediately after pressing Incubate at 20 25 C for 2 5 days Solid Interpretations Since Chloramphenicol in the medium inhibits the growths of other bacteria all 100 Plates 15 11 colonies can be counted for Fungi Storage Keep dry at 4 10 C Do not freeze Formula Potato Extract 3 9 g Dextrose 21 0 g Chloramphenicol 100 mg Agar 14 1 g pH 5 6 0 2 Back to the Index C NISSU PHARMACEUTICAL CO LTD Food Plate X Nissui Quick Microbiological Test for Liquid Foods Code 06231 Solid 10 Plates Directions Take out a plate from the container and dip the plate into liquid specimen at length Take up the plate from the specimen and bring back to the container after excess specimen is eliminated Incubate at 37 C for 24 hours 1 Viable Bacterial count Estimate a viable bacterial count ml by comparing its density of all colonies grown on the surface of modified BHI medium with a control chart 2 Coliform group Estimate Coliform group ml by comparing its density of blue colonies grown on the surface of modified X GAL medium with a control chart Back to the Index 15 12 o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui TC Simple and Easy Dry Medium for Microbial Count Total Viable Bacterial Count Link to User s Manual English Traditional Chinese Simplified Chinese Code a 06740 06741 Directions Compact Dry is ready t
76. e green color by B galactosidase produced by coliform group 100 Packs A Light 2 years 80 Bottles shielding 150 Bottles 1 year 11 9 MUG in the medium is decomposed by B Glucuronidase to be produced by E coli to isolate a fluorescence substance of 4 Methyl Umbelliferone Formula Peptone 5 0 g Sodium Pyruvate 1 0 g Dipotassium Phosphate 4 0 g Potassium nitrate 1 0 g Isopropyl B D 0 1 g Thiogalactopyranoside IPTG 5 bromo 4 chloro 3 indolyl B 0 1 g D galactopyranoside X GAL 4 Methyl Umbelliferyl B 0 1 g D Glucuronide MUG i Sodium Chloride 5 0g Sodium Lauryl Sulfate 0 1g Monopotassium Phosphate 1 0 g pH 7 1 0 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD EC Blue 100 Nissui Bottle Holder Code ae 065 74 For 10 Bottles 1 Back to the Index 11 10 C NISSUI PHARMACEUTICAL CO LTD EC Blue 10 100 Nissui Comparator 06516 10 mL x 1 1 year 05617 100 100 mL x 1 Formula components Indigo Carmine 2 mg o nitrophenol 4 8 mg 4 Methylumbeliiferone 4 mg sodium salt Potassium Dihydrogen Phosphate Log Dipotassium Phosphate 4g Distilled Water 1000 mL Back to the Index 11 11 o gt NISSUI PHARMACEUTICAL CO LTD EC Blue 100 Nissui MPN Plate Code R 06517 Back to the Index 11 12 o gt NISSUI PHARMACEUTICAL CO LTD MacConkey Sorbitol Agar Nissui Isolation medium for E coli 0157 Code ae 05643 Directions Suspend 50 0 g
77. e medium is used for an isolation of Enterococcus in faces water milk dairy products and processed meat Incubate at 37 C for 48 hours E faecalis forms pink or red brown colonies 0 5 2 mm in diameter while E faecium forms yellow colonies Growths of gram negative bacilli are inhibited 100 g Dry RT 3 years Since Enterococcus is an indicator of fecal pollution as in case of Coliform the detection of it is important for the survey of fecal pollution Formula Components Calf Brain Extract Powder 8 5g Heart Extract Powder 8 5g Peptone 10 0 g Dextrose 10 0 g Dipotassium Phosphate 2 5g Brom Thymol Blue 0 032 g FR 0 15 Agar 15 0 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD MEC Broth Nissui Enrichment medium for E coli 0157 Code 05649 Powder 300 g Dry RT 3 years Directions Formula iv cael in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute Peptone 20 0 g necessary amounts of medium into appropriate container Sterilize by autoclaving at 121 C for 15 minutes and Bile Salt 1 12 g then add 20 mg of Novobiocin Incubate at 42 C for 18 24 hours Lactose 5 0 g Dipotassium Phosphate 4 0 g Potassium Dihydrogen Remarks Phosphate oe The medium is used for an enrichment Sodium Chloride 5 0 g culture of E coli 0157 in foodstuffs pH 6 9 Back to the Index 11 15 o gt NISSUI PHARMACEUTICAL CO LTD Sabouraud Agar
78. ecimen in Swab kit tube is dropped into 9 mL sterilized diluting solution Formula Tube contains sterilized Buffered Sodium Chloride Peptone Solution pH 7 0 Nissui Tube is so prepared that total volume dropped is 1mL when all solution is squeezed out Peptone 1 0 g Sodium Chloride 4 3 g Potassium Dihydrogen Phosphate T ka Disodium Phosphate anhydrous 17 236 Back to the Index NISSUI PHARMACEUTICAL CO LTD Sterilized Diluting Solution Nissui OEE E Ee 01553 9mLx4 4x8x4 01551 90mL 20x2 RT 1 Year Formula C t In HE of SE Distilled Water Sodium Chloride 9 0 g Disodium Phosphate 0 0785 g Potassium Dihydrogen Phosphate 904216 Back to the Index 16 10 o gt NISSUI PHARMACEUTICAL CO LTD Opener with Sterilized Diluting Solution Nissui Code 06737 Usage of Opener for Compact Dry Dilution Rack Souse sharp end Stick Opener at right angle with approx 3cm of Flame sterilization Compact Dry Dilution Rack to Opener in ethanol prick Ethanol for disinfection Back to the Index 16 11 o gt NISSUI PHARMACEUTICAL CO LTD Wiping Frame Nissui Code 06598 Back to the Index 16 12 o gt NISSUI PHARMACEUTICAL CO LTD Sterilized Spoid Nissui Code sau 06472 1000 Back to the Index 16 13 o gt NISSUI PHARMACEUTICAL CO LTD Clean Stamp Nissui MSO Agar MSO Microbiological Test for Medical Ambience MRSA Code
79. edium in 1 000 mL of distilled water and heat to dissolve the medium Sterilize by autoclaving at 115 C for 15 minutes Distribute 20 mL amounts of the medium into Petri dishes Prepared medium should be used in a day of preparation or keep in anaerobic condition Powder Remark The medium is developed by Medical School of Gifu University Japan to isolate and culture anaerobic bacteria from clinical specimens The medium is also used for susceptibility tests other than sulfa drugs 300 g Dry RT 3 years Formula Peptone 10 0 g Soya Peptone 3 0 g Proteose Peptone 10 0 g Digested Serum 13 5 g Yeast Extract 5 0 g Meat Extract 2 2 g Liver Extract 1 2 g Dextrose 3 0 g Potassium Dihydrogen Phosphate 258 Sodium Chloride 3 0 g Soluble Starch 5 0 g L Cysteine Hydrochloride 0 3 g Sodium Thioglycollate 0 3 g Agar 15 0 g pH 7 1 8 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD GAM Agar Modified Nissui Gifu Anaerobic Medium Agar Modified For common culture and susceptibility test Code 05426 Directions Suspend 56 7 g of the dehydrated medium in 1 000 mL of distilled water and heat to dissolve the medium Sterilize by autoclaving at 115 C for 15 minutes Distribute 20 mL amounts of the medium into Petri dishes Prepared medium should be used in a day of preparation or keep in anaerobic condition Powder Remark The medium is a modified medium of GAM Agar that is developed by Med
80. en if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose 100 g 1 Year Formula Sodium Chloride 6 800 0 mg Potassium Chloride 400 0 mg Calcium Chloride 200 0 mg Magnesium Sulfate 93 5 mg Sodium Dihydrogen Phosphate NUE Glucose 1 000 0 mg L Arginine Hydrochloride 126 0 mg L Cystine Dihydrochloride H O FLAME L Tyrosine 36 0 mg L Histidine Hydrochloride H O 32 0 mE L Isoleucine 52 0 mg L Leucine 52 0 mg L Lysine Hydrochloride 73 0 mg L Methionine 15 0 mg L Phenylalanine 32 0 mg L Threonine 48 0 mg L Tryptophan 10 0 mg L Valine 46 0 mg Succinic Acid 75 0 mg Sodium Succinate 6H 0 100 0 mg Choline Bitartrate 1 8 mg Folic Acid 1 0 mg Inositol 2 0 mg Nicotinamide 1 0 mg Calcium Pantothenate 1 0 mg Pyridoxal Hydrochloride 1 0 mg Riboflavin 0 1 mg Thiamin Hydrochloride 1 0 mg Biotin 0 02 mg Kanamycin 60 0 mg Titer 19 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Eagle s MEM Nissui Without Kanamycin Phenol Red L Glutamine and Sodium Bicarbonate Autoclavable Powder 05902 Directions Dissolve 9 4 g of the medium in distilled water and adjust the volume to 1 000 mL Sterilize by autoclaving at 121 centigrade for 15 minutes Cool the steril
81. ential medium MEM published by Harry Eagle in 1959 This medium is used as a 2 5 fold concentrated solution for use with small number cell cultures suspension cultures cell colony formation by semisolid agar plaque formation and others Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial medical waste according to the law or regulations related to dispose 1 Year Formula In 0 88 g L L Arginine Hydrochloride r L Cystine Dihydrochloride 1eme L Cystine Hydrochloride 17 56 mg H20 L Histidine Hydrochloride H O 42 0 mg L Isoleucine 52 0 mg L Leucine 52 0 mg L Lysine Hydrochloride 73 0 mg L Methionine 15 0 mg L Phenylalanine 32 0 mg L Threonine 48 0 mg L Tryptophan 10 0 mg L Tyrosine Disodium Salt 44 75 mg L Valine 46 0 mg L Glutamine 292 0 mg Thiamin Hydrochloride 1 0 mg Riboflavin 0 1 mg Pyridoxal Hydrochloride 1 0 mg Nicotinamide 1 0 mg Calcium Pantothenate 1 0 mg Folic Acid 1 0 mg Inositol 2 0 mg Choline Bitartrate 1 8 mg Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD
82. fer 100 mL x 1 D Biotin labeled antibody 150 pL x 1 E eae nasa 8 150 pL x 1 F Chromogenic substrate TMB 12 mL x 1 G Stop solution 12 mL x 1 H Concentrated washing solution 100 mL x 1 I Extraction reagent 50 mL x 1 J Extraction reagent for soybean 100 mL x 1 K Extraction reagent 50 mL x 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD FA test ElA Crustacea Nissui Form Code 08621 08622 Introduction Amendments to Food Sanitation Law in Japan have rendered it mandatory to label 5 specific raw food materials Eggs Milk Wheat Buckwheat and Peanuts identified to have a high possibility of triggering food allergies when foods contain more than a predetermined amount of pg mL or ug g of the specified raw ingredients as of April 2002 Similarly shrimps prawns except lobsters and crawfishes and crabs is one of 20 raw food materials recommended to label conforming to Food Sanitation Law In addition to clearly labeling the above mentioned food products screening of such products is increasingly becoming important in order to confirm the amount of proteins contained in the raw ingredient or in order to confirm the content due to the mixing of these raw materials with other foodstuffs and contamination on production lines FA test ElA Crustacea Nissui is a test kit to determine crustacean derived proteins in foodstuffs precisely based on enzyme linked immunosorbent assay EIA using a
83. g L Isoleucine 51 0 mg L Leucine 51 0 mg L Lysine Hydrochloride 56 5 mg L Methionine 15 0 mg L Phenylalanine 23 5 mg L Proline 15 0 mg L Serine 30 0 mg L Threonine 49 0 mg L Tyrosine 28 0 mg L Tryptophan 7 5 mg L Valine 48 0 mg p Aminobenzonic Acid 0 5 mg Biotin 0 2 mg Choline Bitartrate 0 9 mg Choline Chloride 26 5 mg Folic Acid 1 0 mg Inositol 18 5 mg Nicotinamide 1 0 mg Calcium Pantothenate 0 6 mg Pyridoxal Hydrochloride 0 5 mg Pyridoxine Hydrochloride 0 5 mg Riboflavin 0 15 mg Thiamin Hydrochloride 1 0 mg Cyanocobalamin 0 004 mg Glucose 2 000 0 mg Sodium Pyruvate 110 0 mg Succnic Acid 37 5 mg Sodium Succinate 50 0 mg Hypozanthine 0 025 mg Thymidine 0 013 mg Glutathione 0 5 mg Putrescine Hydrochloride 0 013 mg Dihydroxyethilglycine 1 800 0 mg Phenol Red Sodium Salt 5 0 mg Insulin Bovine 10 0 mg Transferrin 10 0 mg Monoethanolamine 20 0 mg Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD RPMI 1640 Medium Nissui 1 Without Sodium Bicarbonate 05911 Powder Directions Dissolve 10 4 g of the medium in distilled water and adjust the volume to 1 000 mL When there is difficulty dissolving the powder the solution may be acidified to about pH 6 0 with dry ice or CO gas Add a proper amount of sodium bicarbonate pH will be 7 1 7 4 at 37 C in an atmosphere of 5 CO in air by adding 1 0 1 8 g sodium bicarbonate Aseptically add the desire amount of serum Summary RPMI 1640 Mediu
84. g g S L Cysteine Hydrochloride 0 3 g successfully used for cultivation of anaerobic b ee Sodium Thioglycollate 0 3 g acteria It has a broad application to other pH 7 1 microorganisms such as streptococci Back to the Index 8 4 o gt NISSUI PHARMACEUTICAL CO LTD GAM Semisolid without Dextrose Nissui Gifu Anaerobic Medium Semisolid without Dextrose For identification and strain preservation Code an 05460 Powder 100 g Dry RT 3 years Directions Formula Suspend 52 5 g of the dehydrated medium in 1 000 mL of distilled water and heat to Peptone 10 0 g dissolve the medium Distribute the Soya Peptone 3 0 g medium into test tubes 1 5 x 15 cm or 0 9 Proteose Peptone 10 0 g x 15 cm is recommended up to a level Digested Serum 13 5 g slightly higher than the middle about 16 Yeast Extract 5 0 g mL Sterilize by autoclaving at 115 C for Meat Extract 2 2 g 15 minutes and immediately cool down to Liver Extract 1 2 g a stab culture agar Potassium Dihydrogen Store at room temperature in a dark place Phosphate 2 58 Do not store in refrigerator Sodium Chloride 3 0 g L Cysteine Hydrochloride 0 3 g Remark Sodium Thioglycollate 0 3 g The medium is devised as a basal medium Agar 1 5 g for testing the carbohydrate utilization by pH 7 1 anaerobes It is suitable for tests of various biochemical properties motility and Back to the Index preservation of anaerobes 8 5 o gt NISSUI PHARMACEUTICAL CO LTD GAM Semisol
85. g 1ng tube 5 mL 0 05 ng 0 5 ng tube 2 mL 3 years Code an Suspend 7 7 g of the dehydrated medium in about 80 90 mL of distilled water mix well and heat to dissolve the medium and cool Storage down the solution After adjusting the pH to Cap tightly and keep at cool place 2 7 1 add distilled water to make the total 10 C volume 100 mL Distribute into the test tube and double the Formula total volume with the distilled water after Removed biotin from the formula of adding the sample Sterilize by autoclaving Pantothenate Assay Medium and added at 121 C for 5 minutes Inoculate the strain 400 ug of pantothenate instead All other for culture at 37 C for 16 24 hours ingredients remain unchanged Incubate 72 hours for the purpose of pH 7 1 0 1 acidimetry Back to the Index Remarks Strain to be used is Lactobacillus arabinosus strain 17 5 ATCC 8014 available also from Nissui Range of response 0 2 ng 2 ng tube 10 mL 13 10 o gt NISSUI PHARMACEUTICAL CO LTD Powdered Agar Nissui For microbiological usage Code 05835 Powder 500 g Dry RT 5 years Directions Product is purified agar for microbiological usage It rarely contains ingredient that inhibits growth of bacteria and then it fits general usage for microbiological testing And high jelly strength make it economical Remarks Product is purified agar for microbiological testing so that it supports bacteria growth Back t
86. g ol the cenyelated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by Meat Extract 5 0 g autoclaving at 121 C for 15 minutes Peptone 10 0 g The medium are used either slants or plates for routine culture and preservation of Sodium Chloride 5 0 g organisms that are not nutritionally Agar 15 0 g fastidious pH 7 0 Back to the Index 10 8 o gt NISSUI PHARMACEUTICAL CO LTD Nutrient Broth Nissui Code an 05511 Powder Directions Suspend 30 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute the medium into adequate containers and sterilize by autoclaving at 121 C for 15 minutes Remarks The medium aims especially at its transparency and it can be widely used as a broth medium for the cultivation of general bacteria 300 g Dry RT 3 years Formula Meat Extract 5 0 g Peptone 15 0 g Sodium Chloride 5 0 g Dipotassium Phosphate 5 0 g pH 7 0 Back to the Index 10 9 o gt NISSUI PHARMACEUTICAL CO LTD Trypto Soya Agar SCD Agar Nissui Soybean Casein Digest Agar Code an 05516 Granule 300 g Dry RT 3 years Directions Formula fede ica ae es in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by Peptone 15 0 g autoclaving at 121 C for 15 minutes Use Soya Peptone 5 0 g as plates or slants Sodium Chloride 5 0 g Remarks Agar 15 0 g The medium i
87. gainst the specimen Press against the different parts of the specimen when several kinds of Clean Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 30 35 C for 2 5 days Interpretations Count all colonies grown on the surface Remarks Clean Stamp 25 SCD is made and prepared Solidified 30 Plates 150 Plates 17 5 of Code 05516 Trypto Soya Agar Nissui according to its directions and aseptically distributed and solidified in the special Petri dishes having an inside diameter of 58 mm outside diameter of 73 mm and height of 18 mm One clean film bag contains 3 Clean Stamp 25 s connected in series Storage Do keep at 4 10 C Do not freeze Formula Peptone 15 0 g Soya Peptone 5 0 g Sodium Chloride 5 0 g Agar 15 0 g pH 7 3 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Clean Stamp Nissui PSEU Agar PSEU Cetrimide Kanamycin sulfate Nalidixic Acid Agar Microbiological Test for Environment Pseudomonas 06789 Directions Clean Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Clean Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Clean Stamps are tested simu
88. gnesium Sulfate 0 2 g Cetrimide 0 2 g Nalidixic acid 0 015 g Agar 15 0 g pH 7 4 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Cetrimide Agar Nissui For non fermentative gram negative rod Code 05221 Directions Suspend 46 9 g of the dehydrated medium in 1 000 mL of distilled water add 10 mL of glycerin mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute about 20 mL of the medium into Petri dishes Dry the surface of the medium before use Incubate at 30 35 C for 18 72 hours Powder Remarks The medium is developed by Brown and Lowbury to isolate Pseudomonas aeruginosa The medium enrich the production of fluorescent pigment pyocyanin and fluorescein of Pseudomonas aeruginosa which makes a differentiation easy Pseudomonas aeruginosa develops a colony with yellow greenish fluorescent on the medium Both gram positive and gram 300 g Dry RT 3 years negative bacteria other than Pseudomonas aeruginosa are almost all inhibited on the medium Formula Components Peptone 20 0 g Magnesium Chloride 3 0 g Potassium Sulfate 10 0 g Cetrimide 0 3 g Agar 13 6 g pH 7 2 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD ID Test e NF 18 Nissui Identification Test Kit for Glucose Non fermentative Gram Negative bacilli 016 6 6 je z s 3 i f r rar 06629 25 tests 06617 NF Plate
89. he medium at about 55 C Distribute 15 mL of medium into the anaerobic culture pouch in which 10 mL of sample has distributed Mix well and weld to seal the neck of the pouch after removing air bubbles Incubate at 37 1 C for 24 2 hours Powder Remarks The medium is used for the colony counting of Clostridia Spore forming sulfurous acid reducing anaerobic bacterium in foodstuff Clostridia develop colonies with brown to black color by reducing sulfurous acid while others develop light white colonies 300 g Dry RT 3 years Formula Mixed Peptone 15 0 g Soya Peptone 7 5 g Yeast Extract 7 5 g Beef Extract 7 5 g Ferric Ammonium Citrate 1 0 g Sodium Hydrogen Sulfite 1 0 g L Cysteine Chloride 0 75 g Agar 30 0 g pH 7 6 Back to the Index C NISSUI PHARMACEUTICAL CO LTD NGKG Agar Base Nissui Code Granule 05282 Directions Suspend 26 5 g of the dehydrated medium in 900 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Maintain the medium at about 50 C add 100 mL of 20 egg yolk suspension mix well and distribute about 20 mL amounts into Petri dishes For the preparation of egg yolk suspension add 20 mL of egg yolk to 80 mL of sterilized saline solution aseptically and mix well Determinations After drying the surface of the plate smear the specimens and incubate at 30 C for 18 24 hours B cereus forms white and
90. he plate and incubate at 35 C 37 C for 18 24 hours In the following two items after incubation add the following reagents and evaluate IND Add 1 drop of Kovac s reagent VP Add 1 drop of 6 a Naphtol Reagent and also 1 drop of 40 Potassium Hydroxide Compare the results with the appended color chart and determine whether they are positive or negative Determine the species of bacteria by checking the code book provided Formula Kit component 06673 EB 9 Plate 2 test x 25 plates Sterilized Liquid Paraffin 20 mL x 2 tubes 2 tests x 26 Result Form sheets EB 9 Broth 1 5 mL x 50 tubes Kit component 06671 Kov c s Reagent 6 mL x 1 tube 6 a naphthol Test 6 mL x 1 tube solution 40 Potassium Hydroxide 6 mix 1 tube Test solution Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Sterilized Oil Paraffin Nissui for ID Test Code 06608 Liquid 50 mL Back to the Index 3 10 o gt NISSUI PHARMACEUTICAL CO LTD uick ID GN Nissui Identification Test Kit for intestinal bacilli 08720 08721 Reagent Features The QUICK ID GN identification kit is a quick identification system based upon the theories of the microtest and the numerical classification methods It is intended for the identification of clinically significant aerobic gram negative bacilli of human origin with 4 hours according to the positive negative color reactions for 14 biochemical tests includes 12 chr
91. ia it is suitable for the cultivation and preservation of many nutritionally fastidious bacteria The medium does not affect the biological nature of bacteria and it is suitable for precise biological tests 100 g Dry RT 3 years Formula Porcine Brain Extract Powder 4 08 sted Heart Extract 4 0g Peptone 17 5 g Dextrose 2 0 g Sodium Chloride 5 0 g Disodium Phosphate 2 5 g Agar 15 0 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Brain Heart Infusion Broth Nissui Code an 05509 Directions Suspend 35 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Powder Remarks Since the medium has an excellent ability to support the growth of many kinds of bacteria it is suitable for the cultivation and preservation of many nutritionally fastidious bacteria The medium is also used for the cultivation of blood specimens The medium does not affect the biological nature of bacteria and it is suitable for precise biological tests 100 g Dry RT 3 years Formula Porcine Brain Extract Powder 4 08 sted Heart Extract 4 0g Peptone 17 5 g Dextrose 2 0 g Sodium Chloride 5 0 g Disodium Phosphate 2 5 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Nutrient Agar Nissui Code 05514 Granule 300 g Dry RT 3 years Directions Formula Buspend 35 0
92. ical School of Gifu University Japan to isolate and culture anaerobic bacteria from clinical specimens The medium is also used for susceptibility tests other than sulfa drugs Color of the medium is more pale and better transparency 300 g Dry RT 3 years Formula Peptone 5 0 g Soya Peptone 3 0 g Proteose Peptone 5 0 g Digested Serum 10 0 g Yeast Extract 2 5 g Meat Extract 2 2 g Liver Extract 1 2 g Dextrose 0 5 g Soluble Starch 5 0 g L Tryptophan 0 2 g L Cysteine Hydrochloride 0 3 g Sodium Thioglycollate 0 3 g L Arginine 1 0 g Vitamin K 5 mg Hemin 10 mg Potassium Dihydrogen Phosphate 2 5 g Sodium Chloride 3 0 g Agar 15 0 g pH 7 3 8 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD GAM Broth Modified Nissui Gifu Anaerobic Medium Broth Modified For common culture and susceptibility test Code an 05433 Powder 100 g Dry RT 3 years Directions Formula Suspend 41 7 g of the dehydrated medium in 1 000 mL of distilled water and heat to Peptone 5 0 g dissolve the medium Distribute the Soya Peptone 3 0 g medium into appropriate containers Proteose Peptone 5 0 g Sterilize by autoclaving at 115 C for 15 Digested Serum 10 0 g minutes and cool down quickly Do not Yeast Extract 2 5 g shake the medium Meat Extract 2 2 g Liver Extract 1 2 g Remark Dextrose 0 5 g The composition of the medium is exactly Soluble Starch 5 08 same as GAM Agar Modified excluding L Tryptophan 0 2 g agar The medium i
93. ichment medium for adjustment the Solution mL x 2 via SOENEN ala that corresponds to No 0 5 McFarland Kovacs Reagent 6 mL x A vial urbidity standard an Inoculate 0 1 mL of above suspension into each well 18 wells Bren ee esi 6 mL x 1 vial Layer 3 5 drops of sterilized liquid paraffin on 9 certain wells and CUON incubate place the cover on the plate and incubate at 30 or 37 C Zinc Powder 6 mL x 1 vial for 22 24 hours After incubation add 1 drop of Kovacs Reagent IND Nitrate 5 3 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Staphylococcus Medium No 110 Nissui Selective isolation medium for Staphylococci Code 05234 Directions Suspend 150 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute about 20 mL of the medium into Petri dishes Dry the surface of the plate before use Determinations Incubate at 30 C for 48 hours or 37 C for 43 hours Pathogenic staphylococci form yellowish or lemon colored colonies on the medium and have abilities to utilize mannitol to liquefy gelatin positive to Stone reaction to coagulate plasma and to hemolyze rabbit blood Remarks Most of bacteria except staphylococci are inhibited on the medium and some of the non pathogenic staphylococci are also Granule 300 g 6 1 Dry RT 3 years inhibited to some extent Pathogenic sta
94. id Nissui Gifu Anaerobic Medium For cultivation of clinical specimens and preservation of organisms Code an Powder 05424 Directions Suspend 60 5 g of the dehydrated medium in 1 000 mL of distilled water and heat to dissolve the medium Distribute the medium into test tubes Sterilize by autoclaving at 115 C for 15 minutes Store them at room temperature in a dark place Do not store in the refrigerator Determinations Obligate anaerobes dislike the part 5 10 mm below the surface of the medium and grow in the lower part Obligate aerobes grow in the upper part and facultative anaerobes grow throughout the medium However judgement is difficult in case of gas producing bacteria Remark The medium is a semisolid medium devised by reducing the agar content of GAM Agar As the medium permits rapid growth of aerobes as well as anaerobes it is suitable for culture of blood puncture fluid and other various materials and for cultivation and preservation of anaerobes The major character of the medium is to allow active proliferation of anaerobes in the air without using a special apparatus for the anaerobic condition 100 g Dry RT 3 years Formula Peptone 10 0 g Soya Peptone 3 0 g Proteose Peptone 10 0 g Digested Serum 13 5 g Yeast Extract 5 0 g Meat Extract 2 2 g Liver Extract 1 2 g Dextrose 3 0 g Potassium Dihydrogen Phosphate 2538 Sodium Chloride 3 0 g Soluble Starch 5 0 g L Cysteine Hydrochloride 0
95. ies but whole plate sheets become seemingly Yellow lt Salmonella Negative gt There is no color change occurred on the sheet If it were occurred the sheet color would be changed to Red or Reddish purple No Black or Green colonies are observed The sheet color might be changed to Yellow caused by Pseudomonas or Proteus But Yellow portion is small and limited because of their less motility Back to the Index C NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui X BC Simple and Easy Dry Medium for Microbial Count Staphylococcus aureus Link to User s Manual English Traditional Chinese Simplified Chinese Code a 06727 06728 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 40 Plates RT 12 Months 240 Plates colonies eventually are ap
96. ion of the constituents so that sterilization had been L Lysine Hydrochloride 40 0 mg performed by filtration using a membrane filter pease ore 15 0 mg RPMI 1640 Medium Nissui 2 is an autoclavable SEI 15 0 mg powdered medium specially manufactured to L Proline 20 0 mg overcome the difficulties associated with filter L Serine 30 0 mg sterilized media such as contamination by L Threonine 20 0 mg Mycoplasma This medium has growth promoting L Tryptophan 5 0 mg effect to primary cultures of mouse and human L Tyrosine 20 0 mg leukemia cells various kinds of cell lines and L Valine 20 0 mg KATO III cells Biotin 0 2 mg Storage and Expiration Calcium Pantothenate 0 25 mg Close tightly and store at 2 5 C in a dark and dry Choline Chloride 3 0 mg place May be stored up to one 1 year Folic Acid 1 0 mg z Inositol 35 0 mg eee rerit wed Nicotinamide 1 0 mg o ao use the pro uct after its expiry date p Aminobenzoic Acid 1 0 mg Quality of the product is not warranted after its z z shelf life Pyridoxine Hydrochloride 1 0 mg When if medium or reagent touched eyes or Riboflavin 0 2 mg mouth immediately wash with plenty of water Thiamin SAIC cones 1 0 mg and consult a physician Cyanocobalamin 0 005 mg Any medium reagent and materials must be soci EaISUSe 6 000 0 mg sterilized by autoclaving or boiling water after use Potassium Chloride 400 0 mg and then disposed as industrial waste according to Calcium Nitrate 69 5 mg the law or regulations
97. irmly against the specimen Press against the different parts of the specimen when several kinds of Clean Stamps are tested simultaneously Put the cap again immediately after pressing This Clean Stamp is designed to measure and detect the degree of pollution of the specimen which surface has been processed with chemicals or disinfectants As lecithin and polysorbate 80 inactivate those chemicals and disinfectants absorbed Solidified 100 Plates 17 3 into medium more reliable results can be obtained Incubate at 35 C for 24 48 hours Interpretations Count all colonies grown on the surface Storage Do keep at 4 10 C Do not freeze Formula Peptone 15 0 g Soya Peptone 5 0 g Sodium Chloride 5 0 g Lecithin 1 0 g Polysorbate 80 7 0 g Agar 15 0 g pH 7 2 0 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Clean Stamp Nissui Mannitol Salt Agar with Egg Yolk MSEY Microbiological Test for Medical Ambience Staphylococcus Code ne 06783 30 Plates 06782 100 Plates ot te Solidified Directions Clean Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Clean Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Clea
98. ium Citrate 1 0 g Lactose 10 0 g Sucrose 10 0 g Bile Salts 5 0 g Neutral Red 0 02 g Brom Cresol Purple 0 01 g Agar 16 0 g pH 7 4 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD SS Agar with Sucrose Nissui Selective isolation and differential medium for Salmonella and Shigella Code 05032 280 g Granule Dry RT 3 years 05033 70 g x 20 Directions jiet Suspend 70 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL amounts to Petri dishes Dry the surface of the plate until moisture disappears from the surface by leaving the lids of the dishes partly open Do not autoclave Determinations Inoculate the specimens heavily and incubate at 37 C for 20 24 hours Pathogenic bacteria form semitransparent colonies Lactose fermenters form pink or red colonies Also Sucrose fermented Citrobacter does not form black colonies and it can be identify easily Remarks Features The medium inhibits the growth of E coli and miscellaneous bacteria The medium does not require any sterilizing procedures The medium enables to detect pathogenic bacteria easily and its detection rate is far more excellent than other 2 2 The medium requires inoculation of a large amount of feces than in case of other selective media Formula In 70 0 g L Meat Extract 5 0 g Bile Salts 8 5 g Peptone 8 0 g Lactose 10 0 g Sucrose 10 0 g Sodium Ci
99. ium Pyruvate 1 0 g Potassium dihydrogen phosphate tog Dipotassium Phosphate 4 0 g Potassium nitrate 1 0 g Sodium Lauryl Sulfate 0 1g Isopropyl B D 0 1 g Thiogalactopyranoside IPTG 5 bromo 4 chloro 3 indolyl B 0 1 g D galactopyranoside X GAL 4 Methyl Umbelliferyl B 0 1 g D Glucuronide MUG pH 7 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD EC Blue Nissui Test for Coliform group and E coli in water EC Blue 100P Nissui EC Blue 100 Nissui Comparator ald w EC Blue 10 Nissui l Comparator Uninoculated E coli Coliform Distilled wi i U aael Uninoculated Coliform Coliform water 05591 100P For 100 mL 05593 100 For 100 mL 05613 10 For 10 mL Directions A special pouch of EC Blue 100P contains an exact amount of medium for the preparation of 100 mL medium Simple and easy use neither weight measuring nor autoclaving A disposable bottle of EC Blue 100 and EC Blue 10 contains an exact amount of medium for the preparation of 100 mL and 10 mL medium respectively Simple and easy use neither weight measuring autoclaving nor incubation tube Remarks Coliform group change the medium blue to blue green after incubation at 35 37 C for 24 hours Fluorescent is observed when the positive medium is exposed to UV light 365 nm if E coli is present X GAL colorimetric enzyme substrate in the medium is decomposed to bring out blue blu
100. ized medium to room temperature and add a proper amount of sterile 10 sodium bicarbonate solution pH will be 7 1 7 4 at 37 centigrade in an atmosphere of 5 CO in air by adding 12 5 22 0 mL of sodium bicarbonate solution This mixture should be stopper tightly and stored in a cool dark place At the time of use aseptically add 0 292 g of L glutamine sterilized and the desired amount of serum Summary Eagle s MEM Nissui 3 is a powdered medium prepared according to prescription published by Harry Eagle in 1959 Up to now such media were not autoclavable because of decomposition or deterioration of the constituents so that sterilization was performed by filtration using a membrane filter Nissui Pharmaceutical Co Ltd has succeeded in preparing an autoclavable powdered medium This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired This medium has a growth promoting effect for cells such as HeLa or L and other cell lines Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boili
101. l Blue 0 024 g pH 7 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD BGLB Broth Nissui 1 rn Uninoculated Code an 05638 Granule 300 g Dry RT 3 years Directions fermentative bacteria Suspend 40 0 g of the dehydrated medium When gas was evolved after incubation coliform test is positive In case of the examination of water incubate in Lactose Broth first and when the production of gas is observed transplant the culture to BGLB Broth For the test of food and drinks add the specimen directly to the medium and observer the production of gas The medium inhibits the growth of gram positive bacteria while it permits the proliferation of E coli in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute the medium into middle size of test tubes containing a fermentation tube ora Durham s tube Sterilize by autoclaving at 121 C for 15 minutes and cool down rapidly for use Do not use any tubes that contain bubbles in a fermentation tube ora Durham s tube To inoculate the test sample of more than 10 mL use double concentration medium Keep strictly a sterilization time and ensure Formula a rapid cooling before use Slight color discrepancy by lot to lot may be Calf Bile Powder 20 0 g observed Lactose 10 0 g Remarks Peptone 10 0 g The medium is used for testing the Brilliant Green 0 0133 g presence of coliform in food and water and pH 7 0
102. lified Chinese Code a 06744 06745 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 351 C for 24 3 hours interpretations Coliforms grow to develop Blue Blue green colonies as the medium contains chromogenic enzyme substrate X GAL Bacteria other than Coliforms are inhibited to grow and they do not form any colored colonies even if they grow Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui EC Simple and Easy Dry Medium for Microbial Count E coli and Coliforms Link to User s Manual English Traditional Chinese Sim
103. ltaneously Put the cap again immediately after pressing Incubate at 35 C for 24 48 hours Solidified Code sane Interpretations Pseudomonas develops round dome shape or compressed colonies with yellow greenish fluorescent on the medium 30 Plates Storage Do keep at 4 10 C Do not freeze Formula Peptone 20 0 g Magnesium Chloride 3 0 g Potassium Sulfate 10 0 g Cetrimide 0 3 g Kanamycin Sulfate 50 0 mg Nalidixic acid 5 0 mg Glycerin 10 0 ml Agar 15 0 g pH 7 0 0 1 Back to the Index CO NISSUI PHARMACEUTICAL CO LTD Clean Stamp 25 Nissui SCDLP Agar SCDLP Microbiological Test for Environment Viable Bacterial Count Including inactivate agents ee 30 Plates 06793 06792 Directions Clean Stamp 25 SCDLP is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 25 cm Take off the cap of Clean Stamp 25 SCDLP and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Clean Stamps are tested simultaneously Put the cap again immediately after pressing This Clean Stamp 25 SCDLP is designed to measure and detect the degree of pollution of the specimen which surface has been processed with chemicals or disinfectants As lecithin and polysorbate 80 inactivate those chemicals an
104. lucose fermentative The test target bacteria shall be proliferated on enrichment medium Fish colonies from enrichment medium for adjustment the concentration suspension that corresponds to No 3 McFarland turbidity standard Inoculate 0 1 mL of above suspension into each well 18 wells Layer 3 5 drops of sterilized liquid paraffin on 2 certain wells and incubate place the cover on the plate and incubate at 30 or 37 C for 22 24 hours Wells 1 and 2 are empty wells and inoculation of bacterial sample solution is not necessary After incubation add 1 drop of Kovacs Reagent IND 6 a 6 5 25 tests RT naphthol and 40 Potassium Hydroxide Test solution VP into certain wells After addition of specific reagents compare the color developed with the attached color chart and determine as positive or negative results Interpretations Refer N ID Test SP 18 ANALYTICAL PROFILE for identification Formula Kit component 06637 N ID Test SP Plate 1 year 100 tests 25 plates x 18 wells Sterilized Liquid Paraffin 20 mL x 1 vial Result Form 13 sheets x 2 sets N ID Test SP Broth 2 5 mL x 25 tubes Color Chart 1 sheet Kit component 06638 6 a naphthol Test solution 6 mL x 1 vial 40 Potassium Hydroxide e mL xA vial Test solution Nitrite Detection Test 6 mL x 1 val Solution Nitrite Detection Test 6 mLx 1 vial Solution Il Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD ID Teste HN 20 Rapid
105. m Nissui 1 is a powdered medium prepared according to the prescription developed by George E Moore former director of Roswell Park Memorial Institute This medium is developed for suspension culture Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose C NISSUI PHARMACEUTICAL CO LTD 100 g 1 Year Formula L Arginine 200 0 mg L Asparagine H O 56 8 mg L Asparatic Acid 20 0 mg L Cystine Dihydrochloride 65 0 mg L Glutamic Acid 20 0 mg Glutathione 1 0 mg Glycine 10 0 mg L Histidine 15 0 mg L Hydroxyproline 20 0 mg L Isoleucine 50 0 mg L Leucine 50 0 mg L Lysine Hydrochloride 40 0 mg L Methionine 15 0 mg L Phenylalanine 15 0 mg L Proline 20 0 mg L Serine 30 0 mg L Threonine 20 0 mg L Tryptophan 5 0 mg L Tyrosine 20 0 mg L Valine 20 0 mg Biotin 0 2 mg Calcium Pantothenate 0 25 mg Choline Chloride 3 0 mg Folic Acid 1 0 mg Inositol 35 0 mg Nicotinamide 1 0 mg p Aminobenzoic Acid 1 0 mg Pyridoxine Hydrochloride 1 0 mg Riboflavin 0 2 mg
106. m Pyruvate 110 0 mg Thiamin Hydrochloride 0 337 mg Thymidine 0 727 mg Cyanocobalamin 1 36 mg Nicotinamide 0 037 mg Lipoic Acid 0 206 mg Linoleic Acid 0 084 mg Phenol Red 1 24 mg Hanks Solution Nissui 1 Without Sodium Bicarbonate Powder 05905 Directions Dissolve 9 8 g of the medium in distilled water and adjust the volume to 1 000 mL After dissolving completely add a proper amount of sodium bicarbonate pH will be 7 3 7 6 at 37 C in an atmosphere of 5 CO in air by adding 0 2 0 35 g sodium bicarbonate Sterilize by filtration immediately To avoid excessively raising the pH level of the medium it is advantageous to reduce the pH of the medium by gassing with CO before filtration Storage and Expiration Close tightly and store at 2 5 C in a dark dry place May be stored up to two 2 year 100 g 2 Years Formula In 9 8 g L Sodium Chloride 8 000 0 mg Potassium Chloride 400 0 mg Disodium Phosphate 47 9 mg Potassium Dihydrogen Phosphate pecs Magnesium Sulfate 48 8 mg Magnesium Chloride 46 8 mg Calcium Chloride 140 0 mg Glucose 1 000 0 mg Phenol Red 6 0 mg Hanks Solution Nissui Without Phenol Red and Sodium Bicarbonate 05906 Powder Directions Hanks Solution Nissui 2 is a powdered BSS prepared with same composition as Hanks Solution Nissui 1 without phenol red and is used for dialysis experiments of immunology and others Dissolve 9
107. m the surface down to the bottom While in case of the negative reaction the deep or part changes into yellow and only the surface remains violet Determination of Indole production Add Kovac s reagent into the tube The color change of the reagent into red constitutes the positive indole reaction and no color change constitutes the negative one Determination of Motility Motile bacteria cause the turbidity of the whole Powder Og Dry RT 3 years medium or only around the inoculum line while non motile ones grow only along the inoculum line Remarks The medium has been found to be helpful in the identification of Enterobacteriaceae and it also permits simultaneous examination of lysine decarboxylation indole production and motility of bacteria The combination with TSI Agar makes it easy to perform the screening test of the pathogenic bacteria especially the differentiation of Shigella Salmonella and the like Formula Peptone 12 8 g Yeast Extract 3 0 g Dextrose 1 0 g L Lysine Hydrochloride 10 0 g L Tryptophan 0 5 g Brom Cresol Purple 0 02 g Agar 2 7 g pH 6 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD SIM Medium Nissui Confirmation of Enteric bacteria Code 05106 Directions Suspend 35 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 5 mL into small test tubes sterilize by autoclaving at 121 C for
108. medium into appropriate containers Sterilize by autoclaving at 121 C for 20 Formula minutes and immediately cool down for In 28 5 g L use When the medium is kept for stored re heat the prepared medium in boiling L Cystine 0 5 g water to degas before use Sodium Chloride 2 5 g Add the test sample mix thoroughly with Peptone 15 0 g the medium and incubate anaerobically at 30 35 C at least for 14 days If it is Yeast Extract 5 0 g difficult to determine the result transfer a part of the culture into newly prepared medium and sub culture Incubate at 30 Sodium Thioglycollate 0 5 g 35 C at least for 7 days before a determination Dextrose 5 0 g pH 7 1 Remarks Back to the Index The medium is used for the cases in which it is 10 16 o gt NISSUI PHARMACEUTICAL CO LTD TGC Medium without Indicator Fluid Nissui Code 05629 Directions Suspend 30 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 18 mL of the medium into middle size of tubes Sterilize by autoclaving at 121 C for 15 minutes and immediately cool down for use When the medium is kept for stored re heat the prepared medium in boiling water to degas and rapidly cool down before use Granule Remarks The medium is modified by Vera for clinical use and capable to grow wide range of bacteria from aerobic to anaerobic microorganism and also used for blood
109. mula sterilized sodium chloride solution and make homogeneous egg yolk suspension by thorough Heart Extract Powder 5 0g mixing Itis advisable to take the egg yolk from Peptone 10 0 g an egg that weights more than 50 g Sodium Chloride 70 0 g Determinations Lithium Chloride 5 0g Inoculate the specimens on the plate and Mannitol 10 0 g incubate at 37 C for 24 40 hours Agar 15 0 g Coagulase positive staphylococci form clear pH 7 3 yellow or lemon colored colonies In 20 hours of incubation in a short time case or in 40 Back to the Index hours at the longest the colonies opacify the 6 4 o gt NISSUI PHARMACEUTICAL CO LTD N ID Test SP 18 Nissui Identification Test Kit for Staphylococci 06637 06613 SP Plate 06614 SP Broth 06638 SP Reagent Features of N ID Test SP 18 Identification of bacteria is very complicated and there are so many biochemical test need to be done N ID Test SP 18 is based on the theory of the numerical classification method which could receive the combination with 18 kinds of biochemical reactions Only small amount of sample 0 1 mL is needed for each test item Also the kit is stable for 1 year at room temperature Purpose of ID Test NF 18 For identification of various species of Staphylococci Directions for operation of the kit Streak samples onto blood agar or other isolation medium After incubation of the medium confirm colonies will be tested are Gram Negative bacilli and G
110. n of tartrate to the sterilized medium or add 300 g og b F ss d v a Code an Dry RT 2 years chloramphenicol 100 mg L Formula Potato Extract 3 9 g Dextrose 21 0 g Agar 14 1 g pH 5 6 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Lactobacilli Culture Agar Nissui Code 05800 Directions Suspend 59 6 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into the test tube sterilized by autoclaving at 121 C for 10 minutes and use after solidifying in the butt position Stab the medium for culture at 37 C for 24 48 hours White turbidity positive growth will be observed around the stab areas which can be preserved for 6 months in refrigerator More than 1 year preservation is possible with GDO medium Remarks The medium is also used for the preservation of L arabinosus S faecalis and other Lactobacilli Lactobacilli develop the colony with clear portion around the stab area when 5 10 g L of calcium carbonate is added to the Powder 100 g 13 1 Dry RT 3 years medium This makes differentiation of Lactobacilli easy Formula Yeast Extract 5 5 g Peptone 12 5 g Dextrose 11 0 g Potassium Dihydrogen Phosphate 2238 Dipotassium Phosphate 0 25 g Sodium acetate 10 0 g Magnesium Sulfate 0 1 g Manganese Sulfate 5 0 mg Ferrous Sulfate 5 0 mg Agar 20 0 g pH 6 8 Back to the Index o gt NISSUI PHA
111. n Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 C for 24 48 hours Interpretations Staphylococcus aureus Staphylococcus aureus forms round yellow cream color colonies with yellow surroundings around the colonies Due to positive Egg Yolk reaction white turbidity is developed inside the medium around the colonies while the surface has pearly luster In case of faint Egg Yolk reaction is observed extend incubation to 48 hours and read the reaction after colonies are removed Other Staphylococcus Other Staphylococcus forms small round white colonies with no color changes or red surroundings around the colonies and do not show any positive Egg Yolk reaction Storage Do keep at 4 10 C Do not freeze Formula Beef Extract 1 0 g Peptone 10 0 g Sodium Chloride 75 0 g Mannitol 10 0 g Phenol Red 25 0 mg Egg Yolk 30 0 ml Agar 15 0 g pH 7 4 0 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Clean Stamp 25 Nissui SCD Agar SCD Microbiological Test for Environment Viable Bacterial Count Soybean Casein Digest Agar Code nt 06791 06790 Directions Clean Stamp 25 is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 25 cm Take off the cap of Clean Stamp 25 and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly a
112. ne 06781 30 Plates ed Solidified 06780 100 Plates Directions Storage Clean Stamp is a prepared agar medium for Stamp Do keep at 4 10 C Do not freeze method on which agar stands up slightly above the rim of Formula special Petri dish of 10 cm2 Take off the cap of Clean Stamp and gently press the medium against the surface of Components In LL specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the Meat Extract 1 0 g different parts of the specimen when several kinds of Peptone 10 0 g Clean Stamps are tested simultaneously Put the cap Sodium Chloride 40 0 g again immediately after pressing Incubate at 35 C for 24 48 hours Mannitol 10 0 g Int erpret ations Sodium Pyruvate 2 0 g MRSA MRSA forms round yellow cream color colonies See Salona ae with yellow surroundings around the colonies Due to Oxacillin 6 0 mg positive Egg Yolk reaction white turbidity is developed Colistin 10 0 mg inside the medium around the colonies while the surface Amphotericin B 2 0 mg has pearly luster In case of faint Egg Yolk reaction is observed extend Aztreonam 5 0 mg incubation to 48 hours and read the reaction after Phenol Red 25 0 mg colonies are removed Egg Yolk 30 0 ml Non MRSA Mannitol non fermenting no yellow color change and negative Egg Yolk reaction bacteria are Agar 15 0 g not MRSA pH 7 4 0 1 17 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Clean Stamp Ni
113. ne 93 6 mg Choline Bitartrate 7 2 mg Folic Acid 4 0 mg Nicotinamide 4 0 mg Calcium Pantothenate 4 0 mg Pyridoxal Hydrochloride 4 0 mg Riboflavin 0 4 mg Thiamin Hydrochloride 4 0 mg Inositol 7 2 mg Phenol Red 5 0 mg L Glutamine 584 0 mg 19 7 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Dulbecco s Modified Eagle Medium Nissui Without L Glutamine and Sodium Bicarbonate Autoclavable 05919 Powder Directions Dissolve 10 0 g of the medium in distilled water and adjust the volume to 1 000 mL After stirring on a magnetic stirrer for about 30 minutes sterilize by autoclaving at 121 C for 15 minutes Cool the sterilized medium to room temperature and add a roper amount of sterile 10 sodium bicarbonate solution pH will be 7 1 7 4 at 37 C in an atmosphere of 5 CO in air by adding 12 20 mL of sodium bicarbonate solution This mixture should be stopped tightly and stored in a cool dark place At the time of use aseptically add 0 584 g L glutamine sterilized and the desired amount of serum Summary Dulbecco s Modified Eagle Medium Nissui is a powdered medium prepared according to research by R Dulbecco et al Up to now such media were not autoclavable because of decomposition or deterioration of the constituents so that sterilization was accomplished by filtration using a membrane filter Nissui Pharmaceutical Co Ltd Succeeded in preparing an autoclavable powdered medium Thi
114. ned to differentiate Fusobacterium Neomycin and crystal violet have no influence on the growth of Fusobacterium but inhibit Bacteroides and other bacteria Since it is well known fact that almost all gram negative rods which do not form spore are Fusobacterium and Bacteroides the medium is used for the isolation of Fusobacterium Among of non spore forming gram negative anaerobic rods the bacteria grown on the medium is to be identified as Fusobacterium Remarks To prepare the medium heating should be minimum and use the medium in 3 5 hours after preparation Incubate the medium avoiding excess disclosure to the air Powder 100 g Dry RT 3 years Formula Peptone 20 0 g Soya Peptone 1 5 g Digested Serum 6 75 g Liver Extract 0 6 g Meat Extract 6 15 g Yeast Extract 10 0 g Dextrose 3 0 g Potassium Dihydrogen Phosphate 2 58 Sodium Chloride 3 0 g Soluble Starch 5 0 g L Cysteine Hydrochloride 0 3 g Sodium Thioglycollate 0 3 g Neomycin 0 2 g Crystal Violet 0 01 g Agar 14 7 g pH 7 1 8 10 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD GAM Agar with Gentamicin Nissui Code 05450 Directions Suspend 74 0 g of the dehydrated medium in 1 000 mL of distilled water and heat to dissolve the medium Sterilize by autoclaving at 115 C for 15 minutes Distribute about 20 mL of medium into Petri dishes Determination Smear the specimens and incubate under the anaerobic condition Most of
115. ng water after use and then disposed as industrial waste according to the law or regulations related to dispose 100 g 19 3 1 Year Formula In 9 4 g L Sodium Chloride 6 800 0 mg Potassium Chloride 400 0 mg Calcium Chloride 200 0 mg Magnesium Sulfate 93 5 mg Sodium Dihydrogen Phosphate NUE Glucose 1 000 0 mg L Arginine Hydrochloride 126 0 mg L Cystine Dihydrochloride H O FLAME L Tyrosine 36 0 mg L Histidine Hydrochloride H O 32 0 mE L Isoleucine 52 0 mg L Leucine 52 0 mg L Lysine Hydrochloride 73 0 mg L Methionine 15 0 mg L Phenylalanine 32 0 mg L Threonine 48 0 mg L Tryptophan 10 0 mg L Valine 46 0 mg Succinic Acid 75 0 mg Sodium Succinate 6H 0 100 0 mg Choline Bitartrate 1 8 mg Folic Acid 1 0 mg Inositol 2 0 mg Nicotinamide 1 0 mg Calcium Pantothenate 1 0 mg Pyridoxal Hydrochloride 1 0 mg Riboflavin 0 1 mg Thiamin Hydrochloride 1 0 mg Biotin 0 02 mg Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Eagle s MEM Amino Acids and Vitamins Medium Nissui Powder 05904 Directions Dissolve 0 88 g of the medium in distilled water and adjust the volume to 1 000 mL When preparing 2 5 fold concentrated solution heat to 37 C and stir to dissolve After dissolving completely the medium is sterilized by filtration Summary Eagle s MEM Amino Acids and Vitamins Medium Nissui is a powdered medium excluding inorganic salts and glucose prepared according to the minimum ess
116. nium Citrate 1 0 g Brilliant Green 0 0125 g Crystal Violet 0 01 g Agar 15 0 g pH 6 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui Cereus Agar CERE Simple and Easy Stamp Medium for Food Hygiene test Bacillus cereus Code nt 30 Plates 06753 06752 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 24 hours Interpretations Bacillus cereus forms white colonies with an irregular rim which develop opaque zone positive egg yolk reaction around the colonies and change the color of medium to Red Solid 100 Plates 15 8 Small colonies with negative egg yolk reaction are not B cereus Storage Keep dry at 4 10 C Do not freeze Formula Components Heart Extract Powder 5 0 g Proteose Peptone 10 0 g Peptone 10 0 g Sodium Chloride 5 0 g Lactose 10 0 g Glycine 10 0 g Phenol Red 0 05 g Polymyxin B 50 000 units Egg Yolk Suspension 10 Agar 20 0 g pH 7 1 0 3 Back to the Index o gt NISSUI PHARMACEUTICAL C
117. nnnnnnnnnnannnnnanunnannnnnan 2 8 05041 MLCB Agar NISSU aaaaavaaanavnvnnaanvnv nnuv nunnnnnnnannnnnnnnnnannnnnnnunnnnnnnnan 2 9 Enterobacteria Confirmation Differentation and Identification 05104 LIM Agar NiISSUI 2aaaanaaaaav nnnnannnnnnnnnnnnnnnnnnnnannnnnnnnnnannnnnnn nana 3 1 05106 SIM Agar NISSU aaaaaaaaaannanannnnnnnnnnnnnnnunnnnnnnnnnnnnannnnnnnnnnnnnnnnunana 3 2 05148 DNA Agar Nissui aaaaaanaaaaanannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnanaa 3 3 05111 SC Agar NISSU aaauanaaaanannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnnnana 3 4 05107 VP MR Medium N SSU u22a2anaaaanvnaaav nnnnnnnnunnnnnnnnnnnnanunnnnnnnnnna 3 5 05182 ONPG Disk NISSU uuaaanannaavnvnnnnnnnunnnnnnnnnnunnnnnnnnnannnnnnnnnnnnn annan 3 6 05180 Cytochrome Oxidase Test Strip NISSUI aaaaaaaanannaaannnnnnnnnnn 3 7 06626 ID Test EB 20 Nissui 06615 ID Test gt EB 20 Nissui EB Plate 06616 ID Test EB 20 Nissui EB Broth 06628 ID Test EB 20 Nissui EB Reagent naaaaavvnnnannn ra 3 8 C NISSUI PHARMACEUTICAL CO LTD Index Dehydrate Media Enterobacteria Confirmation Differentation and Identification 06673 ID Test EB 9 Nissui 06671 ID Test EB 9 Nissui EB9 Reagent aaa2aannaaaaaaan naannnnra 3 9 06608 Sterilized Oil Paraffin Nissui aaaaaaaaaaanaanannannnnnunnnnnnnnnnnna 3 10 08720 Quick ID GN Nissui 08721
118. nnnnnnunnnnnnnnannnna Compact Dry Nissui EC Compact Dry Nissui EC aaaaaanaaaaaaaanaanunnnnnnnnnnnnnnnnnnnnanunna Compact Dry Nissui YM Compact Dry Nissui YM aaaaaaaaaaannaaanannnnnnnnnnnnnunnnnnnnnnnnnnna Compact Dry Nissui VP Compact Dry NiSSUI VP uaaaaaaaaaannannnnnnnnnnnnnnnnnnnnnnnnnnunnnna Compact Dry Nissui X SA Compact Dry Nissui X SA aaaaaaaaaaanannnnnnnnnnnnnnnnnnnnnnunnnnnnna Compact Dry Nissui SL Compact Dry NiSSUi SL aaaaaaaaaaaana naununnn nnannnnnnnnnnnnnnnnnnannn vi amp NISSUI PHARMACEUTICAL CO LTD Index Prepared Media Compact Dry 06727 Compact Dry Nissui X BC 06728 Compact Dry Nissui X BC 22aaaaaaaaananannnunnnnnnnunnnnnnnnnnnnnnnnnna 16 8 06738 Easy Wiping Kit NiSSUI aaaaaaaaaaaaaaaannanannnunannunnnnnnnnnnnnnnnna 16 9 01553 Sterilized Diluting Solution Nissui 9 mL x 4 01551 Sterilized Diluting Solution Nissui 90 ML 16 10 06737 Opener with Sterilized Diluting Solution Nissui 16 11 06598 Wiping Frame NiISSUI 100 aaaaaaaaaaaaaaannnnnnnnnnunnnnnnnnnnnna 16 12 06472 Sterilized Spoid NISSUI anaaaaaaaanaannnnununnnnnnnnnnnnunnnnnnnnnnnnm 16 13 Clean Stamp 06781 Clean Stamp Nissui MSO Agar MSO 06780 Clean Stamp Nissui MSO Agar MSO 000 17 1 06785 Clean Stamp Nissui SCD Agar SCD 06784 Clean Stamp Nissui S
119. nnunnnnnnnna 10 15 C NISSUI PHARMACEUTICAL CO LTD Index Dehydrate Media Viable bacteria 05610 05629 05602 05618 05622 05625 TGC Medium without Indicator Liquid Nissui TGC Medium without Indicator Fluid Nissui Dextrose Peptone Broth NiSSUI cccccccccceseeseseseseeeeens Standard Method Agar NISSUI aaaaaaanaaaaaaannannunnnnnnnnnna Plate Count Agar with BCP NISSUI aaaaaaaaannaannnannnnnnnn CVT Agar NI SSU uaaaaaaaaannanannnnnnnnnnnnnnnunannnnunnnnnnanunnnnnnnnnna E coli and Coliform 05634 05638 05648 05636 05644 05631 05632 05607 05591 05593 05613 06574 06516 05617 06517 05643 05679 05649 Fungus 05701 05702 05680 05703 05705 Lactose Broth N SSU aaaaanaaaaaaaaannnnnnnnnunnnnnnnnnnnnnnannnnnnnnnnnnna BGLB Broth N SSU aaaaaaaaanaaaaaannnnnnnnnnnnunununnnnnnnnnnunnnnnnnnnnnnnn EC Broth NISSU ananas l vla Nana Naa anna KK KENN Desoxycholate Agar NISSUI uaaaaaanaaaaaasn nnunnnnnnnunnnnnnnnnnnnna EMB Agar NISSEN a2aaaaaaaaaaaaan nannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna X GAL Agar NISSUI aaaaaaaaaaananannnnnnnnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna XM G Agar NISSUI aaaaaanaaaaanannnunnnnnnnnnnnnnnnnnnnnunanannnnnnnnnnnnnnna Blue Light Broth Nissui aauaaaaaaaaaaaaanannnnnnnnnnnnnnnnnnnnnnnnnnanaa EC Blue 100P Nissui EC Blue 100 Nissui EC Blue 10 N SSU a2aaaa
120. nutes Incubate at 25 30 C for 2 days The medium is used for a confirmation test of Enteric bacteria by VP and MR tests Powder Remarks VP Test Add 1 ml of 6 a Naphtol solution and 0 2 ml of 40 Potassium hydroxide and shake vigorously A red color develops after several minutes after it is left on slant position if positive Negative is confirmed if no red color developed even after one hour 100 g Dry RT 3 years MR Test Add 2 to 3 drops of Methyl Red solution A red color develops if positive while a yellow color develops if negative Formula Peptone 7 0 g Dextrose 5 0 g Dipotassium Phosphate 5 0 g pH 6 8 Back to the Index C NISSUI PHARMACEUTICAL CO LTD ONPG Disk Nissui For B galactosidase test 05182 Disk Directions Put a disk in a small test tube and add 1 0 mL of sterilized distilled water Inoculate the organisms which have been cultured on the agar medium and mix well Incubate at 37 C for 18 24 hours and examine the reaction An early decision in 1 2 hours is possible Code an Determination Positive Decide as positive if the solution turns yellow The yellow color is due to o nitrophenyl produced from ONPG by B galactosidase Negative Decide as negative if the solution remain unchanged Remarks The disk enables a simple and rapid B 50 Disks 1 year galactosidase tsdt for examination of properties of bacteria Formula This disk
121. ny sterilizing procedures The medium enables to detect pathogenic bacteria easily pH 7 3 Back to the Index 2 3 o gt NISSUI PHARMACEUTICAL CO LTD DHL Agar Nissui Desoxycholate Hydrogen Sulfide Lactose Agar Code 05040 Granule 300 g Dry RT 3 years Directions Remarks Suspend 63 3 g of the dehydrated medium Though this medium has little selectivity it in 1 000 mL of distilled water mix well and supports excellent growth of Shigella and heat to dissolve the medium Distribute Salmonella that do not grow well on SS about 20 mL amounts to the Petri dishes Agar and is used for the isolation of and dry the surface of the medium before pathogenic coli ai Formula ae nae Incubate at 37 C for about 20 hours Avoid Meat Extract 3 0 g heavy inoculation of the specimen as on SS Peptone 20 0 g Agar The amount of specimen applied to in Lactose 10 0 g case of MacConkey Agar is desirable Sucrose 10 0 g Lactose saccharose non fermenting Sodium Desoxycholate 1 0 g organisms form colorless and transparent Sodium Thiosulfate 2 3 g colonies relatively larger than those on SS Sodium Citrate 1 0 g Agar On the other hand lactose Ferric Ammonium Citrate 1 0 g saccharose fermenting organisms form red Neutral Red 0 03 g and opaque colonies Thus differentiation Agar 15 0 g between the two organisms is easy pH 7 4 Organisms producing hydrogen sulfide are liable to form black colonies Back to the Index 2 4 o gt NISSUI PHARM
122. o the Index 13 11 C NISSUI PHARMACEUTICAL CO LTD Nissui Tube Triple Sugar lron Agar Solid 4 mL x 50 05169 In Tubes Code an Tubes Formula Meat Extract 5 0 g Sodium Chloride 5 0 g Peptone 15 0 g Lactose 10 0 g Sucrose 10 0 g Dextrose 1 0 g Ferric Citrate 0 2 g Sodium Thiosulfate 0 2 g Phenol Red 0 02 g Agar 15 0 g pH 7 3 Back to the Index 14 1 o gt NISSUI PHARMACEUTICAL CO LTD Nissui Tube Sulfide Indole Motility Medium Code Solid 5 mL x 50 Formula Meat Extract 3 0 g Peptone 28 0 g Sodium Thiosulfate 0 025 g Ferric AMmonium Citrate 1 0 g Agar 3 0 g pH 7 2 Back to the Index 14 2 o gt NISSUI PHARMACEUTICAL CO LTD Nissui Tube Lysine Indole Motility Medium Code an Solid 5 mL x 50 05171 In Tubes Tubes Formula Peptone 12 8 g Yeast Extract 3 0 g Dextrose 1 0 g L Lysine Hydrochloride 10 0 g L Tryptophan 0 5 g Brom Cresol Purple 0 02 g Agar 2 7 g pH 6 8 Back to the Index 14 3 o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui Desoxycholate Agar DESO Simple and Easy Stamp Medium for Food Hygiene test Coliform Code ne 06053 30 Plates 06052 100 Plates ott Solid Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic
123. o use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is being gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 35 1 C for 48 3 hours Interpretations The medium consists of non selective medium and redox indicator of 2 3 5 Tripenyl Tetrazolium Chloride TTC Colonies grown on Compact Dry TC are almost all red colored Since some microorganism may not reduce TTC to develop Red Pink color Compact Dry TC may develop colonies that are not necessarily clear red color Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui CF Simple and Easy Dry Medium for Microbial Count Coliforms Link to User s Manual English Traditional Chinese Simp
124. ode 05528 Directions Suspend 16 1 g of the dehydrated medium in 1 000 mL of distilled water Heat to dissolve the medium and dispense into appropriate apparatus according to its purpose Sterilize by autoclaving at 121 C for 20 minutes for use If necessary to dissolve sample 1 10 g Polysorbate 20 or Polysorbate 80 may be added to 1 000 mL of this Buffer Solution The Buffer Solution is used for dissolution or dilution of sample to prepare sample solution Powder 300 g Dry RT 3 years Formula Peptone 1 0 g Sodium Chloride 4 3 g Potassium Dihydrogen Phosphate 356E Disodium Hydrogen Phosphate 236 pH 7 0 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Dorset Egg Medium Nissui For maintenance of organisms Code 05522 Solid Directions Smear on the slant and incubate at 37 C with the rubber stopper loosened In case of Mycobacterium species replace the rubber stopper with a cotton plug which is again replaced with a rubber stopper after several days After growth put the rubber stopper tightly and keep at an adequate temperature Determinations A method of maintenance of organisms The temperature and term for maintenance of organisms on this medium vary with strains of bacteria Maintenance of strains should be conducted in the following technique Mycobacterium Incubate at 37 C and store at room temperature 15 25 C Subculture every 6 months Nocardia Appl
125. of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes After sterilization mix the medium well and distribute about 20 mL of the medium into Petri dishes Determinations After drying the surface of the plate smear urinary specimens quantitatively and incubate at 37 C for 18 24 hours Calculate viable organisms in the specimen by the number of growing colonies on the plate While lactose fermenters such as E coli Klebsiella and Enterobacter form yellow colonies lactose non fermenters such as Proteus and Salmonella form blue colonies Enterococci and staphylococci form small yellow colonies Pseudomonas aeruginosa forms green colonies with a rough periphery Remarks The medium prevents the swarming of Proteus Powder 300 g Dry RT 3 years as it is deficient in electrolyte MacConkey Agar or DHL Agar was often used for the examination of urinary bacteria and these media inhibit the growth of cocci For that reason blood agar and nutrient agar should be used concurrently The medium however permits an accurate count of viable urinary organisms without inhibiting the growth of gram positive bacteria as it contains only 0 002 of brom thymol blue Formula Peptone 4 0g Meat Extract 3 0 g Tryptone 4 0 g Lactose 10 0 g L Cystine 0 128 g Brom Thymol Blue 0 02 g Agar 15 0 g PH 7 3 Back to the Index C NISSUI PHARMACEUTICAL CO LTD TGC Medium
126. of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL of medium into Petri dishes In case of the liquid specimen it is recommended to use the pour plate method to facilitate counting Do not autoclave Granule Determination Coliform bacilli form red colonies after incubation at 35 1 C for 18 22 hours The medium around colonies has light peach or brick colored precipitates Other bacteria are invisible or form extremely small and colorless colonies in general Proteus forms generally large colorless or brown colonies The growth of gram positive coccus is inhibited 300 g Dry RT 3 years Remarks The medium is widely used for the detection of coliform bacilli in water drinks and foods The medium does not require any sterilization Formula Sodium Desoxycholate 1 0 g Peptone 10 0 g Ferric Ammonium Citrate 2 0 g Sodium Chloride 5 0 g Dipotassium Phosphate 2 0 g Lactose 10 0 g Neutral Red 0 033 g Agar 15 0 g pH 7 2 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD EMB Agar Nissui Eosin Methylene Blue Agar Code sane 05644 Directions Suspend 37 5 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute about 20 mL of the medium into Petri dishes Powder Determinations Incubate at 35 1 C for
127. omogenic substrate reactions The bacteria suspension is prepared by mixing a small amount of bacteria one colony The dispensing of bacteria suspension to the plate can be done with one step Only 4 hours aerobic incubation at 35 C 37 C is required Wells on the plate are arranged by a group of three tests for easier coding Directions for operation of the kit The identification is not for use directly with clinical specimens Use isolates from an agar plate such as said Agar plates The usefulness of the identification kit or any other diagnostic procedure performed on clinical specimens is directly influenced by the quality of the specimens themselves It is strongly recommended that laboratories employ methods discussed in the Manual of Clinical Microbiology for specimen collection transport and placement isolation media Procedure Remove plate from pouch and discard a desiccant Take a GN Broth device Using aseptic technique pick one well isolated colony with the tip of a sterile applicator of the device 25 tests 100 tests EON 1 year from said Agar late Suspended a colony in the ID GN Broth device Recap and shake the device for several times to suspend well the conc of McF 0 5 is preferable Pour entire contents of broth tube into the center well of plate Put on the lid and incubate for 4 hours at 35 C 37 C Reading After 4 hours incubation remove the plates from the incubator Refer to
128. ons Suspend 46 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Cool to below 45 C Add 20 mL iodine solution 6 g iodine crystals and 5 g potassium iodide dissolved in 20 mL of distilled water and mix well Distribute adequate amounts into sterilized test tubes Do not heat after addition of iodine solution Prepared medium should be used in a day of preparation Incubate at 42 C for 20 24 hours Powder Remarks The medium is suitable for enrichment of Salmonella in food 300 g 1 4 Dry RT 3 years Formula Casein Peptone 2 5 g Meat Peptone 2 5 g Bile Salt 1 0 g Calcium Carbonate 10 0 g Sodium Thiosulfate 30 0 g Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Rappaport Vassiliadis RV Broth Nissui Selective isolation medium for Salmonella Uninoculated Salmonella Code an 05130 Directions Suspend 26 8 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute adequate amounts into sterilized test tubes or sterilized flask and then autoclave at 115 C for 15 minutes Incubate at 30 35 C for 18 24 hours Powder Remarks The medium is suitable for enrichment of Salmonella in food 300 g Dry RT 3 years Formula Soy Bean Peptone 4 5 g Sodium Chloride 7 2 g Magnesium Chloride 13 58 g Dipotassium Phosphate 0 18 g r Dihydrogen 1 26g Malachite green 0 036 g
129. ontains 3 medium against the surface of specimen Clean Stamp 25 s connected in series The surface of agar is elastic enough to be Storage pressed firmly against the specimen Press Do keep at 4 10 C Do not freeze against the different parts of the specimen Formula when several kinds of Clean Stamps are j 1 tested simultaneously Put the cap again f Peptone 10 0 g immediately after pressing R Dextrose 40 0 g Incubate at 20 25 C for 2 5 days Chloramphenicol 50 mg Interpretations Agar 15 0 g Count all colonies grown on the surface pH 5 9 0 2 Remarks Clean Stamp 25 CPSB is made and Back to the Index prepared of Code 05701 Sabouraud Agar 47 8 o gt NISSUI PHARMACEUTICAL CO LTD FASTKIT SLIM Nissui Sample solution drop section RO Detecting section Test Control 4 oe i o e _ _ e Shelf Life 08630 08631 08632 08633 08634 T WHEAT BUCK WHEAT PEANUT Introduction The Food Sanitation Law obliges that 7 specified allergenic ingredients egg milk wheat buckwheat peanut shrimp and crab which have a high risk of inducing food allergy should be listed on food labels The Food Sanitation Law also recommends that 18 ingredients including soybeans should be listed on the label in a way similar to the specified allergenic ingredients Providing accurate information by listing of ingredients on food labels and preventing contamination with the ingredients
130. ontamination of V parahaemolyticus is suspected when many yellow colonies V Solid 30 Plates 100 Plates 15 5 alginolyticus are observed Storage Keep dry at 4 10 C Do not freeze Formula Yeast Extract 5 0 g Peptone 10 0 g Saccharose 17 0 g Sodium Thiosulfate 10 0 g Sodium Citrate 10 0 g Sodium Cholate 3 0 g Ferric Citrate 1 0 g Sodium Chloride 10 0 g Oxgall 5 0 g Brom Thymol Blue 0 04 g Thymol Blue 0 04 g Agar 15 0 g pH 8 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui TGSE Agar TGSE Simple and Easy Stamp Medium for Food Hygiene test Staphylococcus aureus Code ne 30 Plates 06057 06056 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 48 hours Interpretations Staphylococcus aureus forms black colonies with milky surroundings around the colonies S aureus shows a positive egg yolk reaction Black colony with negative egg yolk reaction is Solid 100 Plates 15 6 not interpreted as S a
131. organisms that grow anaerobically on this medium may be considered as anaerobes However when the specimens are contaminated with gentamicin resistant facultative anaerobes they may grow on this medium Thus it is necessary to confirm if the anaerobes do not grow on this medium under the aerobic condition Remarks The medium was designed for the separation of anaerobes from the specimens that are suspected to be contaminated with aerobes On the medium almost all anaerobes including Bacteroides Fusobacterium Actinomyces Peptococcus Peptostreptococcus Veillonella and Clostridium grow well while many aerobes do not Powder For selective isolation of anaerobes 8 11 300 g Dry RT 3 years The selectivity of the medium is based on the fact that anaerobes are resistant to gentamicin but aerobes are not Formula Peptone 10 0 g Soya Peptone 3 0 g Proteose Peptone 10 0 g Digested Serum 13 5 g Yeast Extract 5 0 g Meat Extract 2 2g Liver Extract 1 2 g Dextrose 3 0 g Potassium Dihydrogen Phosphate 2 5 g Sodium Chloride 3 0 g Soluble Starch 5 0 g L Cysteine Hydrochloride 0 3 g Sodium Thioglycollate 0 3 g Gentamicin 25 mg Agar 15 0 g pH 7 1 Back to the Index C NISSUI PHARMACEUTICAL CO LTD BL Agar Nissui For isolation of anaerobes and differentiation of Bifidobacterium Code an 05430 Directions Suspend 58 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and he
132. oride 5 0 g Lactose 10 0 g Phenol Red 0 05 g Agar 20 0 g pH 7 2 Back to the Index C NISSUI PHARMACEUTICAL CO LTD CW Agar Base without Kanamycin Nissui Clostridium welchii Agar base with Kanamycin For examination of unheated materials Code 05405 Directions Suspend 60 0 g of the dehydrated medium in 900 mL of distilled water mix well and heat to dissolve the medium The heating should be done for less than 90 minutes Maintain the medium at about 50 C add 100 mL of the egg yolk saline suspension Mix well and distribute about 20 mL into Petri dishes When a C perfringens Differentiation Strip is used place the stick into the center of medium before it solidifies To prepare a 100 mL of egg yolk solution mix about 20 g of egg yolk with 80 mL sterilized physiological saline solution Determinations After drying the surface of the plate smear the unheated material and incubate for 15 20 hours under anaerobic condition Clostridium welchii perfringens forms yellowish white round convex colonies with a glistering surface and surrounded by a pearly ray zone opacity of the medium Powder 100 g Dry RT 3 years On the medium added with defibrinated blood instead of egg yolk Clostridium welchii perfringens forms colonies of the same type with a glistering surface and a hemolytic ring When the colonies are exposed to the air in an incubator they will turn green Formula Heart E
133. ot use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose Back to the Index 100 g o gt NISSUI PHARMACEUTICAL CO LTD 1 Year Formula Sodium Chloride 7 599 0 mg Potassium Chloride 224 0 mg Disodium Phosphate 142 0 mg Copper Sulfate 5H 0 0 0025 mg Zinc Sulfate 7H O 0 863 mg Ferrous Sulfate 7H O 0 834 mg Magnesium Chloride 57 19 mg Calcium Chloride 33 31 mg Glucose 1 802 0 mg L Arginine Hydrochloride 211 0 mg L Alanine 8 91 mg L Asparagine H O 15 01 mg L Aspartic Acid 13 31 mg L Cysteine Hydrochloride H O 35 12 mg L Glutamic Acid 14 71 mg L Glutamine 146 0 mg Glycine 7 51 mg L Histidine Hydrochloride H O 20 96 mg L lsoleucine 3 94 mg L Leucine 13 12 mg L Lysine Hydrochloride 36 54 mg L Methionine 4 48 mg L Phenylalanine 4 96 mg L Proline 34 53 mg L Serine 10 51 mg L Threonine 11 91 mg L Tryptophan 2 04 mg L Tyrosine 5 44 mg L Valine 11 71 mg Biotin 0 0073 mg Choline Chloride 14 0 mg Calcium Pantothenate 0 477 mg Folic Acid 1 32 mg Hypoxanthine 4 08 mg Inositol 18 0 mg Putrescine Dihydrochloride 0 161 mg Pyridoxine Hydrochloride 0 062 mg Riboflavin 0 038 mg Sodiu
134. other antibiotics The medium has a fixed quantity of calcium ion and magnesium ion based on the recommendation of WHO and provides a correct inhibitory zone against aminoglycosides and tetracyclines Since the salt concentration affects the diffusion of the antibiotics the salt 300 g Dry RT 3 years concentration in the medium is exactly maintained at 0 8 The medium is also used for an agar plate method Formula Casamino Acid 16 5 g Heart Extract Powder 3 0 g Soluble Starch 1 5 g Dextrose 2 0 g L Tryptophan 0 05 g L Cystine 0 05 g Biotin 5 ug Agar 15 0 g pH 7 3 Back to the Index C NISSUI PHARMACEUTICAL CO LTD Mueller Hinton Agar N Nissui For isolation of general bacteria Code ae 05533 Powder 300 g Dry RT 3 years Directions Formula Suspend 38 0 g oi the cen clares medium in 38 0 g L in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by Beef extract 2 0 g autoclaving at 124 C for 15 minutes Use Casamino add 17 5 g for plates and thickness of medium shall be 4 mm for 9 cm plate pour 25 mL of Soluble starch 1 5 g autoclaved medium Agar 17 0 g Remarks pH 7 3 This medium is also fit for Anti susceptibility testing which applicable for K B method Back to the Index following to NCCLS document M2 A6 10 2 o gt NISSUI PHARMACEUTICAL CO LTD Sensitivity Test Broth Nissui For MIC tests Code an 05534 Directions Suspend 23 0 g
135. own on the medium forms colonies similar to those of pathogenic bacteria Remarks Features The medium inhibits the growth of E coli and miscellaneous bacteria The medium does not require any sterilizing procedures The medium enables to detect pathogenic bacteria easily and its detection rate is far more excellent than other media 300 g Dry RT 3 years Note The medium requires inoculation of a large amount of feces than in case of other selective media Formula Peptone 18 5 g Meat Extract 2 5 g Yeast Extract 1 0 g Lactose 10 0 g Saccharose 10 0 g L Lysine Hydrochlorude 5 0 g Sodium Thiosulfate 2 0 g Ammonium Iron Citrate 1 0 g Sodium Citrate 1 0 g Bile Salt 2 0 g Neutral Red 0 03 g Colorimetric Substrate by 0 2 g Enzyme Agar 15 0g pH 7 0 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD MLCB Agar Nissui For isolation of Salmonella causing food poisoning Code 05041 Powder 300 g Dry RT 3 years Directions Remarks Suspend 49 0 g of the dehydrated medium in The medium is not suitable for an isolation of 1 000 mL of distilled water mix well and heat Salmonella that causes typhoid fever because to dissolve the medium Distribute about 20 it was originally designed for the purpose of mL into Petri dishes without sterilizing and isolation of Salmonella that causes food dry the surface of the medium sufficiently poisoning before use Formula Determinations In 49 0 g L Inocula
136. peared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 352 C for 24 2 hours interpretations Bacillus cereus generates green blue colonies Back to the Index amp NISSUI PHARMACEUTICAL CO LTD Easy Wiping Kit Nissui Easy and convenient swab kit designed for Compact Dry Code EE 06738 Direction for use Unscrew the cap of tube and pull out the cotton swab with which wipe a specific area of surface of specimen material Drop back the cotton swab into the tube and screw tightly Shake or upside down the tube well to mix for original specimen solution Unscrew the top cap of the tube By pressing the middle of tube squeeze all original specimen solution 1 mL out of the tube into 9 mL of sterilized diluting solution which is used as a 10 times diluted specimen for inoculation If necessary prepare further series of diluted specimen with the sterilized diluting solution and inoculate 1 mL of specimen in the middle of Compact Dry Nissui Warning Do not touch the cotton swab with finger Since Compact Dry Nissui detects up to 103 cfu colonies in a plate original specimen 1 mL tube 200 Tubes 2 Years solution may be inoculated direct in Compact Dry without any dilution if number of bacteria were suspected less than 103 cfu About 100 times specimen is easily prepared by adding 2 drops 0 1 mL of sp
137. phylococci generally form yellowish or lemon colored colonies on the medium while non pathogenic strains form white ones The ability for producing pigment utilizing mannitol and liquefying gelatin can be directly examined on the medium Formula Peptone 10 0 g Yeast Extract 2 5 g Gelatin 30 0 g Mannitol 10 0 g Sodium Chloride 75 0 g Lactose 2 0 g Dipotassium Phosphate 5 0 g Agar 15 0 g pH 7 5 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Mannitol Salt Agar Nissui Code an 05236 Directions Suspend 111 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes Distribute about 20 mL of the medium into Petri dishes Dry the surface of the plate before use Determinations Incubate at 30 35 C for 18 72 hours When staphylococci which utilize mannitol grow on the medium they form yellow colonies and turn the medium around the colonies yellow and Staphylococcus aureus can easily be differentiated from non pathogenic staphylococci and other bacteria that do not utilize mannitol and form red colonies Remarks As the medium contains salt of high concentration non halophilic bacteria are inhibited to grow and staphylococci are selectively isolated The screening test of Granule 300 g 6 2 Dry RT 3 years pathogenic staphylococci is performed by checking the ability to utilize mannitol
138. plate sufficiently before use Incubate at 37 C for 16 18 hours Determinations Organisms non fermenting lactose and saccharose such as Salmonella and Shigella form light blue semitransparent colonies On the other hand organisms fermenting lactose and saccharose form purple or pink turbid colonies which makes differentiation between two types of colonies easy Organisms such as Proteus fermenting saccharose form light purple colonies while the colonies of organisms producing hydrogen sulfide turn black more easily than those formed on SS Agar Remarks The medium contains the combination of two dyes i e neutral red and brom cresol purple so that the plate assumes a reddish purple color Thus the medium is distinctive in its usage from SS Agar DCLS Agar and MacConkey Agar Salmonella and Shigella form light blue semitransparent colonies on the medium larger than those on SS agar in a short time of incubation 16 18 hours On the other hand organisms fermenting lactose and saccharose form vividly reddish purple Granule 2 1 Dry RT 3 years 60 g x 20 turbid colonies The medium strongly supports the growth of Salmonella and even S pullorum and S enteritidis which hardly grow on SS agar grow well on this medium There is no need to inoculate the specimen heavily as in case of SS Agar Formula In 60 0 g L Meat Extract 3 0 g Peptone 8 0 g Sodium Citrate 5 0 g Sodium Thiosulfate 2 0 g Ferric AMmon
139. plified Chinese Code a 06742 06743 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets become seemingly colored Put the cap again immediately after inoculation Incubate at 35 1 C for 24 3 hours interpretations Medium contains two kinds of chromogenic enzyme substrate Magenta GAL and X GLUC Red Pink color for Coliforms except for E coli while Blue Blue purple color for E coli are observed respectively Combined total umber of both colonies of Red and Blue is the total number of Coliform group Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Compact Dry Nissui YM Simple and Easy Dry Medium for Microbial Count Yeast and Mold
140. r 24 hours For the detection of thermo stable DNase production run the test according to the simplified Menzie s method For the isolation of Serratia inoculate a small amount of the specimen on the plate and incubate at 37 C for 20 24 hours Positive reaction of DNase production The color of the medium around the colonies turns red or reddish violet Negative reaction of DNase production The color of the medium around the colonies remains unchanged The detection of production of thermo stable DNase should also be interpreted in accordance with the above mentioned criteria Isolation of Serratia is indicated by the distinctive change of positive DNase production Remarks Features As the medium contains toluidine blue O there is no need to add hydrochloric acid onto the colonies for the Powder 100 g 3 3 Dry RT 3 years determination of the DNase production The medium makes it possible to identify rapidly and accurately the thermo stable DNase production that is useful for the detection of pathogenic Staphylococcus It can be used for the isolation of Serratia that is indicated by the distinctive change of positive DNase production Precautions for use The medium is not suitable for isolation of Staphylococcus By adding 1ml of cephalothin solution 100 mg mL to 100 mL of the medium it can be used as the selective medium for Serratia Formula In 42 1 g L Peptone 15 0 g Soya Peptone 5
141. r 8 hours 100 g Dry RT 3 years Formula Peptone 10 0 g Sodium Chloride 10 0 g pH 8 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Vibrio Agar Nissui Isolation of V cholerae and V parahaemolyticus Code an 05201 Directions Suspend 80 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 20 mL of the medium into Petri dishes without sterilization by autoclaving Determinations After drying the surface of the plate inoculate a larger amount of specimen than in case of other selective media and incubate at 37 C for 18 20 hours Saccharose fermentative bacteria such as Vibrio cholerae and Vibrio alginolyticus form blue colonies due to the color development of water blue Vibrio parahaemolyticus forms slightly reddish and translucent colonies Remarks The medium is a modification of DEC agar medium and is useful for the isolation of both V cholerae and V parahaemolyticus The composition of taurocholate and sodium lauryl sulfate permits the growth of the pathogenic Powder 300 g 4 3 Dry RT 3 years vibrios while the addition of the indicators such as cresol red and water blue makes the color change of the colonies clear due to the fermentation of saccharose Formula In 80 0 g L Yeast Extract 5 0 g Peptone 5 0 g Sucrose 12 5 g Sodium Taurocholate 5 0 g Sodium Lauryl Sulfate 0 2 g Sodium Citrate
142. related to dispose Monosodium Phosphate 677 0 mg Magnesium Sulfate 48 8 mg Glucose 2 000 0 mg Back to the Index Phenol Red 5 0 mg o gt NISSUI PHARMACEUTICAL CO LTD ES Medium Nissui With Kanamycin Without L Glutamine and Sodium Bicarbonate Autoclavable 05971 Powder Directions Dissolve 9 7 g of the medium in distilled water and adjust the volume to 1 000 mL Sterilize by autoclaving at 121 C for 15 minutes Cool the sterilized medium to room temperature and add a proper amount of sterile 10 sodium bicarbonate pH will be 7 1 7 4 at 37 C in an atmosphere of 5 CO in air by adding 12 5 22 0 mL of sodium bicarbonate solution This mixture should be stopped tightly and store in a cool dark place At the time of use aseptically add 0 292 g L glutamine sterilized and the desired amount of serum Summary ES medium Nissui is a powdered medium prepared on the basis of the prescription published by H Koyama et al In this medium Eagle s MEM is supplemented by seven amino acids sodium Pyruvate and Cyanocobalamin and the medium is autoclavable similar to Eagle MEM Nissui Storage and Expiration Close tightly and store at 2 5 C in a dark dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty
143. ride 84 0 mg L Cystine Dihydrochloride 70 3 mg Glycine 30 0 mg L Histidine Hydrochloride H O HS L Isoleucine 104 8 mg L Leucine 104 8 mg L Lysine Hydrochloride 146 2 mg L Methionine 30 0 mg L Phenylalanine 66 0 mg L Serine 42 0 mg L Threonine 95 2 mg L Tryptophan 16 0 mg L Tyrosine Disodium Salt 89 5 mg L Valine 93 6 mg Choline Bitartrate 7 2 mg Folic Acid 4 0 mg Nicotinamide 4 0 mg Calcium Pantothenate 4 0 mg Pyridoxal Hydrochloride 4 0 mg Riboflavin 0 4 mg Thiamin Hydrochloride 4 0 mg Inositol 7 2 mg Phenol Red 5 0 mg Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD SFM 101 Nissui Basal medium 12 5 g Supplement A 10 mL Lyophilized Supplement B 5 mL 05963 Directions Completely dissolve the entire contents of the basal medium 12 5 g in approximately 900 mL of distilled water Reconstitute Supplement A with 10 mL of distilled water whereas Supplement B is ready to use as is Add both Supplement A and Supplement B into the basal medium and add sodium bicarbonate to the medium If 1 0 1 5 g of sodium bicarbonate is added to one liter of the final medium the pH of the medium at 37 C 5 CO will be 7 1 7 4 Add distilled water to bring the total volume of the medium to 1 000 mL After thorough mixing immediately sterilize the final medium by membrane filtration Keep prepared medium at 2 10 C before use Storage and Expiration Close tightly and store at 2 5 C in a dark and dry pla
144. s Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose 100 g 9 4 g x 10 Packs 19 1 1 Year 2 Years Formula In 9 4 g L Sodium Chloride 6 800 0 mg Potassium Chloride 400 0 mg Calcium Chloride 200 0 mg Magnesium Sulfate 93 5 mg Sodium Dihydrogen Phosphate 115 0 mg Glucose 1 000 0 mg L Arginine Hydrochloride 126 0 mg L Cystine Dihydrochloride H O 31 4 mg L Tyrosine 36 0 mg L Histidine Hydrochloride H O 42 0 mg L Isoleucine 52 0 mg L Leucine 52 0 mg L Lysine Hydrochloride 73 0 mg L Methionine 15 0 mg L Phenylalanine 32 0 mg L Threonine 48 0 mg L Tryptophan 10 0 mg L Valine 46 0 mg Succinic Acid 75 0 mg Sodium Succinate 6H O 100 0 mg Choline Bitartrate 1 8 mg Folic Acid 1 0 mg Inositol 2 0 mg Nicotinamide 1 0 mg Calcium Pantothenate 1 0 mg Pyridoxal Hydrochloride 1 0 mg Riboflavin 0 1 mg Thiamin Hydrochloride 1 0 mg Biotin 0 02 mg Kanamycin 60 0 mg Titer Phenol Red 6 0 mg Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Eagle s MEM Nissui Q With Kanamycin Without Phenol Red L Glutamine and Sodium Bicarbonate Autoclavable
145. s Sodium Lauryl Sulfate 0 2 g Coliforms 0 4 c Count all blue blue purple color colonies grown on the surface as E S brom R hloro 3 indolyl p 0 1 g coli D glucuronide X GLUC E coli 0 157 does not have a B glucuronidase and then it will be 5 bromo 6 chloro 3 indolyl B D 0 1 g identified as a Coliform galactopyranoside MAGENTA GAL Z Overtime incubation may foster growth of microorganisms other than Agar 15 0 g E coli and Coliforms Red color may be observed if the sample contains lactobacilli that has pH 7 0 0 2 also a B galactosidase Storage Back to the Index Keep dry at 4 10 C Do not freeze 15 4 o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui TCBS Agar TCBS Simple and Easy Stamp Medium for Food Hygiene test Vibrio parahaemolyticus Code ne 06055 06054 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 24 hours Interpretations Green V parahaemolyticus and Yellow V alginolyticus colonies are observed on the surface Possible c
146. s Vibrio cholerae and Vibrio alginolyticus form turbid yellow colonies while saccharose non fermentative bacteria such as Vibrio parahaemolyticus form colonies with a greenish blue center Fecal streptococcus and Proteus may sometimes grow but their colonies are very small and the differentiation between them is easy Remarks As the medium has high selectivity the growths of gram positive bacteria and gram negative non pathogenic bacteria in feces are inhibited Powder Package Shelf Life 300 g 4 5 Dry RT 3 years The procedure is very easy because sterilization is not required Note It may be better to inoculate a larger amount of specimen than in case of the other selective media Formula In 86 0 g L Yeast Extract 5 0 g Peptone 10 0 g Sucrose 17 0 g Sodium Thiosulfate 10 0 g Sodium Citrate 10 0 g Sodium Cholate 3 0 g Ferric Citrate 1 0 g Sodium Chloride 10 0 g Oxgall 5 0 g Brom Thymol Blue 0 04 g Thymol Blue 0 04 g Agar 15 0 g pH 8 8 Back to the Index C NISSUI PHARMACEUTICAL CO LTD X VP Agar Nissui Code 05135 Directions Suspend 102 4 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute immediately about 20 mL of the Powder medium into Petri dishes Do not autoclave Dry the surface of the medium before use Determinations Inoculate the specimens directly or after enrichment culture and incuba
147. s fit for various purposes pH 7 3 0 2 because it permits the growth of fastidious bacteria that do grow on Nutrient Agar or Heart Infusion Agar and is markedly Back to the Index superior to conventional media as a basal medium of blood agar 10 10 o gt NISSUI PHARMACEUTICAL CO LTD Trypto Soya Broth SCD Broth Nissui Soybean Casein Digest Broth Code an 05630 Directions Suspend 30 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute into appropriate containers and sterilize by autoclaving at 121 C for 15 minutes Granule Remarks The medium is commonly used for the cultivation of fastidious bacteria such as Neisseria Brucella streptococci and pneumococci As the bacteria grow rapidly on the medium they tend to die out soon So the medium is not suitable for use a stock culture medium It is not desirable to subculture pneumococci streptococci and Neisseria repeatedly on this medium For blood culture inoculate about 5 ml of blood specimen into 50 ml of the medium 300 g Dry RT 3 years It is possible to isolate the bacteria from bacteremia and septicemia Formula Peptone 20 0 g Sodium Chloride 5 0 g Dextrose 2 5 g Dipotassium Phosphate 2 5 g pH 7 3 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Buffered Sodium Chloride Peptone Solution pH7 0 Nissui For preparation of sample solution C
148. s liquid and used for L Cysteine Hydrochloride 0 3 g isolation and cultivation of anaerobic Sodium Thioglycollate 0 38 bacteria from clinical specimens The L Arginine 1 0 g medium is also used for susceptibility tests Vitamin K 5 mg other than sulfa drugs in particular for the Hemin 10 mg micro liquid dilution method of anaerobic Potassium Dihydrogen 2 5 g bacteria Phosphate Sodium Chloride 3 0 g pH 7 3 8 3 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD GAM Broth Nissui Gifu Anaerobic Medium Broth For common culture and susceptibility test Code 05422 Powder 300 g Dry RT 3 years Directions pneumococci and meningococci Suspend 59 0 g of the dehydrated medium in The medium is also suitable for blood culture 1 000 mL of distilled water and heat to dissolve the medium Distribute the medium Formula into test tubes or flasks and sterilize by In 59 0 g L autoclaving at 115 C for 15 minutes Peptone 10 0 g For cultivation of anaerobic bacteria cool the Soya Peptone 3 0 g medium immediately after the sterilization in Proteose Peptone 10 0 g running water without shaking or moving Digested Serum 13 5 g a e onda hee a Yeast Extract 5 0 g balas da fe sneeroblceandiions Moat Extract 228 Liver Extract 1 2 g Dextrose 3 0 g Remark Potassium Dihydrogen This is a liquid medium for anaerobic Phosphate 2 5g Ka pears is setae k k ste Agar Sodium Chloride 3 0 g dlee ea erani a hee henin ies Soluble Starch 50
149. s medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired Compared with Eagle s MEM this medium contains about twice the concentration of amino acids and about four times the concentration of vitamins It contains Glycine and L serine which are nonessential amino acids Pyruvate which is important for glycolysis and a very small amount of ferric ion It is nutritious for the growth of cultured cells and is widely used for research on viruses primary cell cultures and single cell cultures Storage and Expiration Close tightly and store at 2 5 C in a dark and dry place May be stored up to one 1 year Precautions Do not use the product after its expiry date Quality of the product is not warranted after its shelf life When if medium or reagent touched eyes or mouth immediately wash with plenty of water and consult a physician Any medium reagent and materials must be sterilized by autoclaving or boiling water after use and then disposed as industrial waste according to the law or regulations related to dispose 100 g 19 8 1 Year Formula n 9 5 g L Sodium Chloride 6 400 0 mg Potassium Chloride 400 0 mg Calcium Chloride 200 0 mg Magnesium Sulfate 97 7 mg Sodium Dihydrogen Phosphate Sl Ferric Nitrate 9 H O 0 1 mg Glucose 1 000 0 mg Sodium Pyruvate 110 0 mg Succinic Acid 106 0 mg Sodium Succinate 6 H O 27 0 mg L Arginine Hydrochlo
150. slightly thick colonies with an irregular margin on the medium and shows the lecithinase reaction In case of the lecithinase reaction their colonies form a zone of opacity and the medium around the colonies presents a red color The growth of miscellaneous bacteria except B cereus may be inhibited Even if some bacteria grow their colonies are small and 300 g 9 1 Dry RT 3 years do not show the lecithinase reaction Remarks The medium was designed for the selection and detection of B cereus from the contaminated foods On the medium the spores of B cereus are formed well After incubation at 30 C for 18 hours the sporulation can be determined by observing them microscopically Formula Peptone 1 0g Yeast Extract 0 5 g Sodium Chloride 4 0 g Glycine 3 0 g Polymyxin B Sulfate 50 000 units Phenol Red 0 025 g Agar 18 0 g pH 6 8 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Sensitivity Disk Agar N Nissui For sensitivity test Code 05530 Directions Suspend 38 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 115 C for 15 minutes Distribute 20 mL into Petri dishes to attain a thickness of 4 mm A round Petri dish with diameter of 9 cm gives 4 mm thickness with 20 mL medium Powder Remarks The medium is a modification of Mueller Hinton medium and does not inactivate sulfa drugs and any
151. ssui SCD Agar SCD Microbiological Test for Environment Viable Bacterial Count Soybean Casein Digest Agar Code ne 30 Plates 06785 06784 Directions Clean Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Clean Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Clean Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 C for 24 48 hours Solidified Interpretations Count all colonies grown on the surface Storage Do keep at 4 10 C Do not freeze Formula Peptone 15 0 g Soya Peptone 5 0 g Sodium Chloride 5 0 g Agar 15 0 g pH 7 2 0 1 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Clean Stamp Nissui SCD Agar with Lecithin Polysorbate 80 SCDLP Microbiological Test for Environment Viable Bacterial Count Including inactivate agents Code ne 30 Plates 06787 06786 Directions Clean Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm2 Take off the cap of Clean Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed f
152. such as Triphenyl Tetrazolium Pseudomonas Acinetobacter K 0 05 g Flavobacterium Alcaligenes and Coliforms Chloride TTC develop red colonies by reducing TTC Agar 15 0 g Growths of gram positive bacteria are inhibited pH 7 0 OThe least heating is must to keep quality Stop heating immediately after the medium 10 21 o gt NISSUI PHARMACEUTICAL CO LTD Back to the Index Lactose Broth Nissui Uninoculated Code an 05634 Directions Suspend 18 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute the medium into middle size of test tubes including a fermentation tube or a Durham s tube Sterilize by autoclaving at 121 C for 15 minutes and cool down rapidly for use Do not use any tubes that contain bubbles in a fermentation tube or a Durham s tube To inoculate the test sample of more than 10 mL use twice or three times thicker medium Granule Remarks The medium is used for testing the presence of coliform in food and water and is also used for the determination of lactose fermentative bacteria When no gas was evolved after incubation 300 g Dry RT 3 years coliform test is negative In case of positive gas evolution color of medium changes to yellow further confirmation test with BGLB Broth and or EMB Agar should be performed Formula Components Meat Extract 3 0 g Peptone 10 0 g Lactose 5 0 g Brom Thymo
153. te at 35 37 C for 18 24 hours Vibrio parahaemolyticus form blue or blue green colonies Vibrio cholerae and Vibrio vulnificus form purple colonies Vibrio alginolyticus form milky colonise Remarks As the medium has high selectivity the growths of bacteria except Vibrio in feces 300 g Dry RT 3 years are inhibited Formula Peptone 10 0 g Yeast Extract 5 0 g Sucrose 30 0 g Sodium Thiosulfate 6 4 g Sodium Citrate 10 0 g Sodium Chloride 20 0 g Sodium Pyruvate 5 0 g Bile Salt 3 0 g Selective Agents 0 27 g Colorimetric Substrate by 0 25 g Enzyme Agar 12 5 g pH 8 8 4 6 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD NAC Agar Nissui For non fermentative gram negative rod Code an 05220 Directions Suspend 35 7 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium An autoclaving is prohibited Incubate at 35 C for 24 48 hours Powder Remarks The medium is developed to isolate Pseudomonas aeruginosa The medium enrich the production of fluorescent pigment pyocyanin and fluorescein of Pseudomonas aeruginosa which makes a differentiation easy Pseudomonas aeruginosa develops a colony with yellow greenish fluorescent on the medium Both gram positive and gram negative bacteria other than Pseudomonas aeruginosa are almost all inhibited on the medium 300 g Dry RT 3 years Formula Peptone 20 0 g Dipotassium Phosphate 0 3 g Ma
154. te the specimens directly or after the Yeast Extract 5 0g enrichment and incubate at 37 C for 18 Peptone 10 0 g 24 hours Heart Extract Powder 2 0 g Salmonella produces hydrogen sulfide and Sodium Chloride 4 0 g forms black colonies while the growths of Mannitol 3 0 g some strains of S typhi and S paratyphi A is z z fi ae L Lysine Hydrochloride 5 0 g strongly inhibited so the medium is z R Sodium Thiosulfate 4 0 g unsuitable for isolation of them R Ferric AMmonium Citrate 1 0 g Furthermore the growth of bacteria except Brilli G 0 0125 Salmonella is strongly inhibited and even rilllant reen 5 though they grow on the medium their Crystal Violet 0 01 g colonies never turn black Therefore Agar 15 0 g differentiation is easy pH 6 8 Back to the Index 2 9 o gt NISSUI PHARMACEUTICAL CO LTD LIM Medium Nissui Lysine Indole Motility Medium Code 05104 Directions Suspend 30 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute about 5 mL into small test tubes Sterilize by autoclaving at 121 C for 15 minutes Then cool immediately and use as a semisolid butt Determinations Take the bacteria with a straight wire and stab into about one third of the depth of the medium Incubate at 37 C for 18 24 hours Determination of Lysine decarboxylation The positive reaction is indicated by violet color except light or faded violet fro
155. the Color Chart for an interpretation Positive or Negative of the reaction except for the item of ORO PYR IND and NIT These 4 items require the following additional procedure at room temperature to judge PRO and PYR Add a drop of Cinnamaldehyde reagent and allow it to stand still for 5 minutes IND Add a drop of Koac s reagent into the well of GC I NIT Add a drop of Nitrate reduction reagent into the well GL N and allow it to stand still for 5 minutes Formula Kit component 08720 Quick ID GN Plate 25 plates Quick ID GN Broth 1 mL x 25 tubes Result Form 26 sheets Kit component 08721 Kov c s Reagent 6 mL x 1 tube Cinnamaldehyde Reagent 6 mL x 2 tubes Nitrate Reduction 6 mixi tube Reagent Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Salt Polymyxin Broth Nissui For enrichment of Vibrio parahaemolyticus Code 05215 Directions Suspend 33 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Distribute adequate amounts into test tubes or flasks and then sterilize by autoclaving at 121 C for 15 minutes The amount of specimen is about one tenth of the medium Incubate at 37 C overnight then take a loopful of the culture and inoculate on a plate of the selective medium such as TCBS Agar Note Incubation for 8 hours may be enough in an urgent case Do not incubate for more than 24 hours to prevent the growth of other bacteria
156. trate 8 5 g Sodium Thiosulfate 5 5 g Ferric Citrate 1 0 g Neutral Red 0 025 g Brilliant Green 0 33 mg Agar 13 5 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD SS Agar Nissui Selective isolation and differential medium for Salmonella and Shigella ne Code 05021 240 g Granule Dry RT 3 years 05020 60 g x 20 Directions k detection rate is far more excellent than other Suspend 60 0 g of the dehydrated medium in 1 000 mL of Note distilled water mix well and heat to dissolve the medium The medium requires inoculation of a large amount of Distribute about 20 mL amounts to Petri dishes Dry the feces than in case of other selective media surface of the plate until moisture disappears from the surface by leaving the lids of the dishes partly open Formula Determinations Meat Extract 5 0 g Inoculate the specimens heavily and incubate at 37 C for Bile Salts 9 0 g 20 24 hours Pathogenic bacteria form semitransparent colonies Peptone 7 5 g Lactose fermenters form pink or red colonies Some Lactose 10 0 g strains of Proteus produce hydrogen sulfide and turn Sodium Citrate 8 5 g black Proteus grown on the medium forms colonies similar to i pathogenic bacteria Sodium Thiosulfate 5 5 H Ferric Citrate 1 0 g Remarks F Neutral Red 0 025 g sFeatures The medium inhibits the growth of E coli and Brilliant Green 0 33 mg miscellaneous bacteria Agar 13 5 g The medium does not require a
157. unaaaaaann nnuunnnnnnnunnnnnnnnnnnnnunnnnnnnnunnnn nuna EC Blue 100 Bottle Holder NiSSUI aaaaaaanaaaaaaaaannnnnnnnnna EC Blue 10 Nissui Comparator EC Blue 100 Nissui Comparator aaaaaaaaanaanaaannanunnnnnnn EC Blue 100 Nissui MPN Plate cccccssssseeeeeeees MacConkey Sorbitol Agar NiSSUI aaaaaaannaaaannnnnnnnnnnnnuna EF Agar Base NiSSUI aaaauanaaaaanaanannnnnnnnnnnnnnnnunnnnnnnnnnnnnnnna MEC Broth N SSU aaaaaaaanaanaaanaannunnnnnnnnnnnnnnnnnnnnnnnnnanunnnnna Sabouraud Agar N ISSU aaaaaaanaaaaaaanannnnnnnnnnnnnnnnnnnnnnnnnnnnnna Corn Meal Agar NISSU aaaaaaaaaaaaaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna AC Broth Base NISSU 2aaaaaaaaaaanaaaannnnnnnnunnnnnnnnnnnnnnnnnnnnnnnunana Candida GE Agar Nissui aaaaaaanaaaaaaanananunannnnnnnnnnnnnnnnnnnnnnnna Czapek Dox Agar NISSUI aaaauaaaaanannannunnnnnnnnnnnnnnnnnnnnnnnnunnnna iv o gt NISSUI PHARMACEUTICAL CO LTD Index V o gt NISSUI PHARMACEUTICAL CO LTD Dehydrate Media Fungus 05706 Malt Agar NiSSUI aaaaaaaaaaaaaaanannnnnnunnnnnnnnnnnnnnnunnnnnnnnunnnnnnanan 12 6 05709 Potato Dextrose Agar NISSUI aaaaaanaaaaaan nannannnnnnnannnnnunnnna 12 7 Vitamin 05800 Lactobacilli Culture Agar NiSSUI aaaaaaanaaaaana nnnnnnnnnnnnnnnna 13 1 05801 Lactobacilli Inoculum Broth NiISSUI aaaaaaaaaaaaan nannnnnnnnnna 13 2 05802 B12 Culture Agar NISSUI aaaaaanaaaaa
158. ureus Storage Keep dry at 4 10 C Do not freeze Formula Meat Extract 3 0 g Peptone 15 0 g Soya Peptone 2 0 g Sodium Chloride 65 0 g Lithium Chloride 5 0 g Calcium Chloride 1 0 g Mannitol 5 0 g Glycine 5 0 g Potassium Tellurite 0 1 g Egg yolk 5 Agar 15 0 g pH 7 0 0 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Food Stamp Nissui MLCB Agar MLCB Simple and Easy Stamp Medium for Food Hygiene test Salmonella Code ne 30 Plates 06751 06750 Directions Food Stamp is a prepared agar medium for Stamp method on which agar stands up slightly above the rim of special Petri dish of 10 cm Take off the cap of Food Stamp and gently press the medium against the surface of specimen The surface of agar is elastic enough to be pressed firmly against the specimen Press against the different parts of the specimen when several kinds of Food Stamps are tested simultaneously Put the cap again immediately after pressing Incubate at 35 37 C for 24 hours Interpretations Salmonella produces hydrogen sulfide and forms black colonies or colonies with black center Citrobacter may develop black colonies just like Salmonella Solid Purple colonies are not Salmonella Storage Keep dry at 4 10 C Do not freeze Formula Yeast Extract 5 0 g Peptone 10 0 g Heart Extract Powder 2 0 g Sodium Chloride 4 0 g Mannitol 3 0 g L Lysine Hydrochloride 5 0 g Sodium Thiosulfate 4 0 g Ferric AMmo
159. ve Disodium Phosphate 6 45 g heating do not autoclave Mannitol 5 0 g Potassium Dihydrogen Add the specimen of about one tenth of the Phosphate 2 0 g medium and incubate at 37 C for 3 hours Stir them well after the first incubation and Bile Powder 20 0 g continue the incubation for 20 24 hours Brilliant Green 0 0135 g or more Finally take a loopful of the culture and subculture on an adequate pH 7 2 selective medium Back to the Index Remarks The medium is quite suitable for preliminary culture of Salmonella in food and feed 1 2 amp NISSUI PHARMACEUTICAL CO LTD Selenite Cystine Broth Base Nissui Selective Enrichment Medium for Salmonella Code an 05009 Powder 100 g Dry RT 3 years Directions Formula Suspend 19 0 g of the dehydrated medium nee eee and 4 0 g of Sodium Selenite in 1 000 mL Peptone 5 0 g of distilled water mix well to dissolve the medium Heat the mixture to dissolve if Soe 4 08 necessary Do not autoclave nor heat too Sodium Phosphate 10 0 g long The medium can be used for selective SORME Coie growth culture of Salmonella in not only pH 7 0 clinical specimen but also foodstuffs and water Remarks The medium is ready to use simply after mixing to dissolve If not dissolved well heat up If it is heated too long a red turbidity is produced Back to the Index C NISSUI PHARMACEUTICAL CO LTD Tetrathionate Broth TT Nissui Code 05132 Directi
160. which are not listed on the labels are required to prevent food allergy Moreover as a measure to prevent contamination routine management at the manufacturing sites including verification of the process of washing the machines and devices used for production is important Product Features 1 The simple one step operation enables easy assessment and anybody can perform the test easily 2 Because there is no need for special test equipment and the test gives rapid results in 15 minutes it is best for routine management at the production sites 20 Tests 18 1 1 Year Operating Procedures for Testing 1 Leave the test strip in the aluminum pouch at room temperature and remove from the pouch immediately before use 2 With an oil based marker pen write the name of the test sample or the number of the subject under test on the absorbent pad of the test strip removed from the pouch 3 Place the test strip carefully on the flat stand and drop a 100 uL portion of the sample solution onto the sample solution drop section Otherwise dispense a 150 uL portion of the sample solution into a test tube and attach the test strip to the test tube so that the sample solution drop section of the test strip is immersed in the sample solution Formula A Test Strip 2 Tests x 10 Packs B Dilution Buffer 50 mL x 1 Bottle C Extraction Buffer 1 10 Concentration Komis dee Back to the Index C NISSUI PHARMACEUTICAL CO LT
161. xtract 5 0 g Proteose Peptone 10 0 g Peptone 10 0 g Sodium Chloride 5 0 g Lactose 10 0 g Phenol Red 0 05 g Kanamycin Sulfate 0 2 g Agar 20 0 g pH 7 2 Back to the Index o gt NISSUI PHARMACEUTICAL CO LTD Bacteroides Agar Nissui For differentiation and isolation of Bacteroides Code an 05440 Directions Suspend 74 0 g of the dehydrated medium in 1 000 mL of distilled water mix well and heat to dissolve the medium Sterilize by autoclaving at 121 C for 15 minutes and distribute the medium into Petri dishes Dry the surface sufficiently before use It is desirable to use the plate within 3 5 hours after preparation Determinations Differentiation The medium is able to identify Bacteroides from the organisms that are isolated from clinical specimens and confirmed to be asporogenic gram negative bacilli of obligate anaerobes Inoculate the bacilli into GAM Semisolid Nissui and incubate for 24 48 hours Take the organisms streak the surface of the plate and incubate at 37 C for 24 48 hours under an anaerobic condition The organisms are identified as Bacteroides when they grow well on this plate Selective isolation When clinical specimens are considered to be lightly contaminated with other organisms Bacteroides can be isolated selectively by smearing them directly Fusobacterium does not grow on this medium but some aerobic cocci may grow on it For the quantitative culture dilute a
162. y Dry Medium for Microbial Count Vibrio parahaemolyticus Link to User s Manual English Traditional Chinese Simplified Chinese Code a 06746 06747 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 1 mL of specimen in the middle of dry sheet of Compact Dry except Compact Dry SL Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored Dried 16 5 40 Plates RT 18 Months 240 Plates colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Put the cap again immediately after inoculation Incubate at 35 2 C for 18 20 hours Interpretations The medium contains chromogenic enzyme substrate X Phos that develops Blue color for many yeast and antibiotics that inhibit the growth of bacteria Mold forms the cottony colonies with characteristic color Some of yeast does not develop Blue color on Compact Dry YM Back to the Index o gt NISSUI PH
163. y Medium for Microbial Count Salmonella Code 06732 06733 Directions Compact Dry is ready to use and portable plate containing dried sheet medium Detach necessary number of plate from a set of four by bending up and down while pressing the lid It is available for solid food staff homogenize with buffer solution water or liquid sample and swab test Drop 0 1mL of enriched specimen at approx 1 cm from the edge of plate gently After inoculating the specimen drop 1mL of sterilized water at the opposite point where specimen dropped Water shall diffuse automatically Inoculated sample diffuses automatically and evenly into the plate dried sheet medium is transforming to gel form Specimen should be diluted by buffer solution to the level of concentration of less than 300 cfu plate If bacteria more than 10 cfu were inoculated on a plate no colonies are formed and no colored colonies eventually are appeared on the plate but all plate sheets becomes seemingly colored Dried 16 7 40 Plates RT 18 Months 240 Plates Put the cap again immediately after inoculation Incubate at 41 43 C for 20 24 hours Interpretations lt Salmonella Positive gt Black to Green isolated or fused colonies are observed and sheet around the colonies is changed to Yellow If a large quantity of Salmonella is inoculated on a plate no isolated colonies are formed there may be several spots with fused Black or Green colon
164. y in the same method as in case of Mycobacterium except for subcultivation of every 3 months Meningococci Incubate at 37 C and store at 4 C Enterobacteriaceae Subculture Salmonella once every year and Shigella or Escherichia coli every 6 months Notes This medium is not suitable for the preservation of organisms which produce protease for example Vibrio and Pseudomonas 100 tubes 5 years Remarks This medium is the modification of the egg medium described by Dorset for isolation and cultivation of Mycobacterium species The modification is performed by replacing broth with physiological saline solution Use for the following purposes Maintenance of organisms By using the medium strains can be preserved with the least variations of properties such as S R variations colicinogenicity colicin tolerance and drug sensitivity Pure culture of Mycobacterium species Determination of proteolytic activity of prganisms Formula Fresh Egg Mixture yolks and whites 800 mL 0 9 Sodium Chloride mae Solution pH 7 5 0 3 This medium is prepared by distributing 3 mL amounts into narrow test tubes solidifying into a slant after sterilization Back to the Index C NISSUI PHARMACEUTICAL CO LTD CLED Agar Nissui Cystine Lactose Electrolyte Deficient Aagar Isolation and quantitative culture of urinary organisms Code 05527 Directions Suspend 36 2 g of the dehydrated medium in 1 000 mL

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