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BEELINE 220s User Manual

1. Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 2 Glossary The following is a list of some of the terms used within this manual which are specific to the Xsp 220 and its software Area Definition The process of defining XYZ co ordinates for any item located within the Working Area of the instrument Application This is a flexible program written specifically for a laboratory application Software Area File A file containing XYZ co ordinates and other data describing an item such as a rack on the instrument so that the Probe can be go to specific positions within that rack DT Probe An assembly comprising a pipetting needle a pressure sensor tube a tip adaptor spring Level Sensor Level Sensor and locking nut An electronic circuit which detects liquid when the probe needles are immersed in conductive liquid or a change of pressure with a disposable tip A connector that forms part of the probe which connects the probe needles to the level Plug sensor circuit Level Sensor A threaded back of the instrument which connects to the level sensor tubing on the probe connector Pipetting Needle The metal needle that is connected to the Syringe Pump Probe Guide A black clamp which holds the Probe in position in the Z Rack but allows some Probe Nozzle movement The tapered narrow end of a Probe Needle which protrudes from the bottom of the tip adaptor Protocol A user definable file containing various para
2. 5 1 Overview This is the process of entering the co ordinates for any racks containers microplates etc that the instrument needs to use including the Washbowl Tip Racks and the Waste Tip position In outline the process involves moving the probe to certain key co ordinates located on the bed and saving the data in a file called an Area File This data can then be used whenever this item needs to be accessed by the probe Area Definition is performed using the program SetArea or SetArea32 depending on version which is loaded by selecting the icon on the desktop The end result of the process is the creation of a number of files containing the precise coordinates of the various racks used by your instrument 5 2 Area Files Used by the DT Application The table below lists the default names given to the Areas required by the DT Application All of these Area files need to be present in the main sample processor directory CAKWINSP Although these names can be changed it is recommended that you leave them unaltered until you have familiarised yourself with the Applications software as any changes here also need to be reflected in the Applications themselves A Default copy of all of these will all be installed as part of the software installation procedure so you do not have to create these from scratch However you will at least need to check them and possibly edit them slightly AREA NAME ITEM USAGE NO
3. When the instrument calculates that it has to follow liquid it tracks the liquid down in a number of steps The size of each step is controlled by the Insertion Depth specified in the protocol If this is relatively large eg 4mm the pump will be able to aspirate a larger volume of liquid before the probe needs to moves down This will tend to increase the speed of processing The disadvantage is that a larger proportion of the needle s exterior surface will become contaminated Note that the depth is maintained at a minimum of 75 of the original insertion depth Figure 44 khows the probe movements during a large aspiration volume Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 1 This shows the probe needle at the specified insertion depth prior to aspiration 2 Shows the liquid level after the first aspiration Note that the probe remains 1mm beneath the surface 3 The probe then moves down again to a depth of 4mm and is ready to aspirate more liquid Note that if the distance between the Minimum Liquid Height and the current probe height is at any point less than the specified Insertion Depth the instrument will give an Insufficient Volume error In order to minimise the Dead Volume the insertion depth should be reduced to a minimum However be aware that aspirating a large volume with a small Insertion Depth increases the number of Steps required to perform the aspiration so it will reduce the throughput 5 6 1 1
4. Area File A file containing the XYZ co ordinates which defines how the machine will use an Area An Area File will contain a set of 1 or more XY co ordinates which define 1 or more positions where each position can be used in the same way An Area file may be used by more than one protocol to access the same rack for different processes provided that they can be performed at the same set of XYZ co ordinates A rack may be defined with more than one Area file so that it can be used for different functions if the co ordinates are not identical An example of this is where one process may require the probe to pipette at the bottom of a rack at the centre of a tube and another may require it to pipette at the top of the same rack and close to edge of the tube This would require two separate Area Files to be defined for the same physical rack Area Format This describes the basic shape or configuration of the rack Options include Rectangular e g test tube rack or an array of reagent bottles Singleton a single reagent bottle plus other specials such as Washbowl Most Areas can be defined as variants of the Rectangular format Location A part of an Area comprising one or more positions or rows Depending on the complexity of the rack being defined an Area may consist of one Location or multiple Locations An array of positions can only be defined as a single location if the following is true The distance between each position wi
5. In fact the XSP 220 software is capable of distinguishing between numeric and alphanumeric well number formats in any worklist that is read into the system so it is only when generating worklists from scratch or appending data to an existing worklist that the software needs to know what format to use Bisi wall sam Mitel Figure 85 If you wish to change the well number format from numeric to alphanumeric or vice versa click Change The Change Source Well Number Format dialog will appear Select soece weil mambe fori Figure 86 Click the button corresponding to the format you require You will return to the Source Well Number Format window 8 5 4 2 Target Area Well Number Format This can be specified in exactly the same way as for the Source well number format 8 6 Saving Changes Finally when all changes have been made choose the Save and Exit button Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 9 Maintenance and repairs 9 1 Overview In order to maintain the performance of the sample processor at its optimum level certain operations are necessary on a daily basis and others weekly or every fifty to one hundred hours of operation Refer to Appendix B for recommended maintenance schedule It is assumed that spillage will be dealt with immediately and so maintenance of this kind is not scheduled The instructions in this chapter must be followed carefully to avoid problems with short term reliabilit
6. Start Copying Files prompt The Visprog software will now be installed on the computer in the CAKWINSP directory After re booting the computer the following 6 icons will be displayed on the desktop 6 ERE Configuration Diagnose32 Runtime32 Guardian Set read2 Visprog3z2 Spreadsheet Figure 18 The 6 programs shown above in the program selection window represent the main VisProg System Software programs They have the following functions PROGRAM NAME FUNCTION CONFIGURATION Used to set up various instrument and software options such as password definitions which Serial Port to use and whether to use the software on line or off line etc DIAGNOSE32 This shortcut should be deleted as it is not applicable to this system RUNTIME32 The program used to actually drive the Xsp 220 GUARDIAN SPREADSHEET Program used for processing Worklists SETAREA32 Used for defining the co ordinates of racks VISPROG2 or VISPROG32 on earlier Used for creating new applications requires dongle This shortcut versions should be deleted as it is not applicable to this system Note that some of the shortcuts refer to programs that are not used by the by this specific instrument and may be deleted Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 4 Application Installation New Installation 4 4 1 Running Set up After installation of the Visprog2 disk you must now install the Application software A
7. We Their Sie nl bee hesh R AA DEN pe SL ee 4 6 4 3 Display The Display option configures the colour displayed for various liquid handling errors that may arise during processing These colours are displayed when the instrument runs Although configurable it is recommended that you use the default values The No Error condition should always be configured to be a different colour from the other error conditions Taba bmi j lus Legs a T g cal ir Laia Cicas rr a Tiara o Figure 26 4 6 4 4 Language This should be set to Neutral for English No other languages are implemented at present Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Taba mip Comte Leegenga We Tiro hen Lis iam get aia i 4 6 4 5 Weighing This option must be unchecked as it is not available for this application Tasker ain Demian Lange Vier Time her Paca Comer 4 6 5 Trace Select the Trace options tab If you want Trace data files to be created for linking to a plate reader then check this Create Trace Files option This will create a text file with the extension ddd in the path specified Ignore the remaining options Identifiers Database Miscellaneous etc as these are currently only relevant to systems incorporating an automated bar code reader wi Prem AT AT prr ST a E PT a od de T Figure 27 Document 9967 901 Issue 1 HTZ Xsp 220 Operato
8. above bottom of Height used as the lowest point the probe tube tube should ever go to Table 1 Recommended positioning of the probe when defining the Z parameters for different Areas N A Not applicable i e value is not used when processing this Area Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Height definitions for the Source and Target Area files JULEP EVIL IN VEEL ELH Figure 39 Height definition for Source and Target Area Files 1 Traverse Height amp Clearance Height Set approx 5 10 mm above top of the tube so that probe will clear tubes when moving across rack 2 Search Height Set approx 1 2 mm above highest possible liquid level in tube 3 Dispense Height1 Not used for Samples Set inside tube but above maximum possible liquid level 4 Minimum Liquid Height Set about 1mm above base of tube Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual The process described below is with reference to TIP1000 Area but is applicable to the defining of the co ordinates required by any single rectangular array as per a standard test rack or Microplate but itis applicable to any of the other rectangular areas such as DTSAMPLES and DTDEEPWELL etc 5 4 7 Locations The top right section of the Area Definition window shows Locations For an array of positions to be defined as a single Location the distance between the positions within
9. 2002 96 EC 12 4 RoHS Directive 2002 95 EC 85 13 WARRANTY 00 Nn 14 USEFUL CONTACTS Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 1 Introduction 1 1 Basic Overview of the Xsp 220 DT Disposable Tip Detect amp Eject block Archive Plate Disposable Tip Rack 96 Position Sample Rack Figure 1 The XSP 220 DT sample processor comprises an XYZ platform fitted with a single Z drive and an integrated syringe drive connected to a probe fitted with a disposable tip DT option The DT option comprises a special block mounted under the arm that incorporates a Tip Detector and a Tip Ejector block The Tip Detector is used to confirm that tips are picked up and ejected correctly during processing Pipetting is achieved by means of a special probe that picks up disposable tips via a Tip Adaptor that screws onto the end The probe is connected to the syringe by means of the Pipetting Tubing In operation the pipetting tubing may be filled with air or distilled water depending on whether the instrument is used in Dry or Wet mode In either mode it is the movement of the syringe piston that causes liquid movement in the disposable tip Liquid level detection is achieved by means of a special pressure sensor mounted at the back of the instrument The pressure changes that occur when a tip moves into liquid are monitored by this sensor It is connected to the Tip Adaptor by a second length of tubing of s
10. Kent TN13 2PX United Kingdom Tel 44 0 1732 763555 Fax 44 0 1732 763530 The volumes available are 250ml Concentrate 2 Litre Concentrate 5 Litre Ready to use For your local distributor please refer to www medichem co uk Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Appendix A Maintenance Schedule Wk No Start of day Daily Details Mon Tues Wed Thurs Fri Sat Empty Vacuum Waste bottle Empty Washbowl waste container Re fill System fluid Run Start of Day Protocol Clean Probe Nozzles with Methanol Sensitivity Check Utilities protocol Ensure all racks are secured on the bed Check Washbowl operation End of day Daily Details Run End of Day Protocol Clean Probe Nozzles with Methanol Weekly Details Clean Z rack if necessary Run End of Day Protocol Run Decontaminate with Medis Dist H20 Notes Tick the relevant left hand box and signature the right hand box Wk No Start of day Daily Details Mon Tues Wed Thurs Fri Sat Empty Vacuum Waste bottle Empty Washbowl waste container Re fill System fluid Run Start of Day Protocol Clean Probe Nozzles with Methanol Sensitivity Check Utilities protocol Ensure all racks are secured on the bed Check Washbowl operation End of day Daily De
11. a Row must all be the same For Locations with more than 1 row the distance between all Rows must also be the same however the inter row distance does not need to be the same as the distance between positions within a Row The data that is entered into the remaining two fields Positions Row and Rows Location depend on the size and type of rack being defined In the case of the TIP1000 area it is usually defined as a Location with 8 positions Row and 12 Rows Location as this Area is usually defined as either the rack containing the 96 1 2 ml dilution tubes or as one or more Microplates Most of the Areas used by this application are of a Rectangular type with a single location However some Areas can have multiple locations e g a 4 position microplate rack Each plate has to be defined as an individual location 5 4 7 1 Inserting a new Location in an Area File This is performed using the Insert Button at the bottom right of the window First move to the Location where you want the new Rack Inserted If the Area only has a single Location Defined ignore this step Press the Insert Button This will insert a Rack immediately after the Current Rack It will default to have the same number of Positions Row and Rows Location as the previous Location This can be changed manually if required Pal Aue Vettes LIME If you want to delete a Location select the Rack you want to remove and press the Delet
12. a positive location Adjustable foot Figure 4 After levelling the instrument you should then check that the arm is level with respect to the deck This is easier to do after the probe has been installed so this will be describer first Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 6 Installing the probe amp tubing connections 3 6 1 Installing the DT probe The probe assembly comprises the probe spring locking nut and tip adaptor The probe contains two lengths of tubing of different diameters The smaller is used for pressure measurements and the larger is used as a fluidic connection to the syringe Probe Tip Figure 5 Disposable Tip DT Probe and Tip components and Tip Adaptor Probe Collar Probe Collar Groove Probe Guide 9972 502 Probe Guide Pin Probe Guide Grubscrew Z Rack 9972 260 Figure 6 Install the black probe guide onto the top of the Z rack Note that the guide pin Figure 6 and grubscrew face towards the back of the instrument The grubscrew should be aligned with the indentation at the top rear of the Z rack It is located at the bottom of the probe guide do not install upside down Insert the probe into the guide ensuring that the groove in the collar aligns with the guide pin at the rear Ensure that the probe is inserted fully into the Z rack and is located at its lowest possible position Slide the spring onto the probe and secure with the Locking Nut Tighten
13. an outer edge of the instrument as shown in Figure 14 Waste Tip Bag Support 3476 Waste Tip Bags MME 9361 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 7 3 Installing the Sample 8 Deepwell Rack This is located on the 2 rack locators on either the front or rear locating rail The rack comprises 96 sample tube positions and a single SBS format microplate location at the rear The 4 black anodised locating bars are adjustable to accommodate the slight differences in the sizes from different manufacturers Rack Locating bars Adjustment point for locating bars Figure 15 3 7 4 Electrical Connections The power cord may now be fitted into the back of the instrument Leave the instrument switched off Plug in the 25 way end of the RS232 cable into the socket as shown in igure 10 3 7 5 Checking the arm is level To achieve optimum performance and reliability it is essential that the instrument arm is level The most critical location is the disposable tip rack as any differences here can result in unreliable tip operation It is therefore recommended that you check that the probe tip is completely level 0 2mm with the top of the tip rack across all 96 positions 1 Make sure the instrument is switched off When it is switched on the instrument motors are energised and it is difficult to move the arm manually 2 Make sure that the tip rack is in position and on its rack locators securely Ensure t
14. by modifying the critical heights by 5 or 10 steps up or down as appropriate 9 5 Unblocking the DT Probe Sensor Tube To operate correctly it is important that the DT Tip adaptor and the DT Sensor tube remain dry The sensor tube is a very fine tube and can get wet if the probe is accidentally immersed in liquid Once this has happened you may get intermittent liquid handling errors in particular failure to detect liquid In order to correct this you need to 1 Remove the tip adaptor and dry it completely especially the interior bore 2 Blow air through the Sensor Tube to remove any liquid that may have become trapped in its internal surfaces This is done by disconnecting the probe pressure sensor connector from the instrument and connecting it up to the 20m1 disposable syringe included in the installation kit using the coupling provided see Fill the syringe with air before connecting it and once connected blow the air through the probe Disconnect and repeat 3 to 5 times to ensure that any liquid has been expelled 3 Reconnect the probe connector to the Pressure Sensor Port and refit the Tip Adaptor Figure 87 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 10 Spare Parts List The following is a list of some of the essential spares and consumables that may be required Please note that none of these items listed as Demo Consumables are covered as part of the Warranty It is recommended that you have these item
15. contain a full set of all the Areas required by the software only the ones that are defined and used during testing You may find that these files still need to be fine tuned as the positioning of the arm may have moved slightly during transit The exact positioning may also be influenced by the flatness of the bench that the Xsp 220 has been installed on 4 6 Instrument Configuration 4 6 1 Default Configuration Configuration After completing the installation of the software the PC will now be configured to the factory setting However it may be necessary to change one or more of these parameters before the instrument will run correctly In particular this applies to the Com Port setting There are also number of other options you may like to change just to suit your specific requirements These changes are made using the Configuration program which is loaded by clicking on the Configuration icon on the desktop For completeness this section describes all of the main configuration options that you would need to check if installing without a factory configuration disk However if you have one you can usually just check the Com Port setting and skip the rest as they should all be correct Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 6 2 Instrument Type Once the configuration program is loaded there are a number of menu options Select the Instrument Type as Other Models in Figure 19 Instrument Type
16. location ahowe Mac wrge inben in locas shown Figure 52 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 6 5 2 Archiving with Worklist In this application the source tube identifiers are obtained from a separate file CSV or XLS You will be prompted to specify the Worklist located in the C XSP Pending directory that contains the barcodes for the samples you are about to process You will not be prompted for the number of samples as this is specified within the file The processing information is also added to this file so you will not be prompted for A name of data file to create Other prompts will be as per the Archiving application Figure 53 6 5 3 Load Samples This application is used to create a worklist only You will be prompted to enter the number of samples and the name of the worklist file to create You will then be prompted to enter the barcodes of the individual tubes in the same way as per the Archiving application b 5 1 The Worklist file will be saved but will not be processed To process the Worklist you will need to run the Archiving with Worklist application 6 5 4 Load Samples Sequential This application is also only used for creating a Worklist Instead of identifying the tubes you will be prompted to enter a seed barcode This acts as the starting point for a series of identifiers that increment by 1 A code will be automatically generated for each sample requested and stored
17. log Records all processing steps step by step Tube Data This is the Worklist Rack Data N A TubeID N A TubeStatusShadow N A TipData Pressure measurements recorded during processing 6 7 Pausing and Stopping the Xsp 220 1 Emergency Stop If it is apparent that the instrument is liable to spill liquid damage other samples damage itself or appear to become out of control then switch OFF the power to the XSP 220 immediately In less urgent cases proceed as below 2 You may pause the instrument at any time by pressing the red STOP button The instrument Stop will pause and an option will be displayed which allows you to Abort Processing If you want to continue then select the No option Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 An alternative way of pausing is to use the F10 function key on the computer keyboard Press it once and the machine will pause Press a second time and the instrument will resume processing 4 Note that the instrument will always complete the move or processing step it is currently performing before pausing so it may be a few seconds before it actually stops especially if it is in the middle of doing a probe wash Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 7 Fault Finding 7 1 Liquid Handling Fault Finding table The following table lists some of the common problems that may arise when running the instrument These are most likely to occur when the i
18. objective of defining or editing the coordinates for the different Areas is achieved by moving the probe using the Virtual Joystick to certain key positions and then recording the X amp Y or Z coordinates for the probe in that position Pressing this button will enter the Current Coordinate into the field Ta Mutual Jogatek R A where the cursor is positioned Current X Y and Z Coordinate i e the probe where it is currently located Figure 35 The Virtual Joystick has 8 X Y control arrows and 2 Z control arrows The 3 fields below the arrows show the steps recorded by during the X Y amp Z movements from the start Home position These steps equate to the following linear traverse movements in millimetres i X Motion 8 steps equal 1mm of traverse movement ii Y Motion 8 steps equal 1mm of traverse movement iii Z Motion 22 5 steps equal 1mm of up down movement Therefore with reference to the example shown in Figure 34 the Z movement from the home position down to the Search Height is 2081 steps The actual linear distance moved is therefore 2081 22 5 92 5 mm approximately To move the probe simply move the cursor above one of the arrows and press The probe will start to move slowly in small steps in the direction of the arrow The size of the steps will gradually increase and the probe will accelerate If you want to change back to a small movement at any time release the mouse button wait 2 seconds before pressing aga
19. on the Desktop 2 Chose File gt Open This will display a list of the Area Files in the KWINSP directory 3 Select the file TIP1000 by highlighting it and by double clicking it You will now see a window which usually displays 4 separate blocks of data Figure 34 This is all the information currently stored on disk for this Area Another window also appears entitled the Virtual Joystick This allows you to move the probe in the direction of the arrow and capture its coordinates in the Area Definition Window As an example if you chose TIP1000 you will see something like this Mili hemp a Tua Loca Pri Thieme E BE H anen Currently defined coordinates as saved in the Area file im od Fermi Fica ai i tl Feal Picea Y bim Pervert Heyl T Erd of Pie E de Demem Hpi Krmak Eagle Depame He git AE ua Lep Hegi Figure 34 Normally when checking the coordinates of pre defined Areas you only need to concern yourself with the Height Data and the X Y coordinates The parameters defined in the Area Information and Locations are therefore dealt with later Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 4 1 Using the Virtual Joystick Once the SETAREA program is loaded the instrument can be controlled by the Virtual Joystick This window allows you to drive the instrument incrementally in any of the 3 axes simply by pressing on one of the arrow keys with the cursor The basic
20. right hand side of the instrument with the instrument facing you The green power light on the front panel of the instrument should be illuminated Switch on the computer and wait for the software to load If using Wet mode ensure that the System Fluid Diluent bottle is filled with PBS Ensure the Waste bottle is empty and that the cap is securely tightened Check that the Open gravity waste bottle connected to the washbow is not full Make sure that all racks are securely in position and located properly on their rack locators Pay particular attention to the Tip rack and ensure that it is positioned properly on its locators and that the ridges on the green plastic locating tabs are clicked into position and are beneath the stainless steel retaining clip Figure 46 Double click on the Run Xsp 220 icon This loads the RunTime software that drives the instrument From the Protocols Menu at the top of the screen select the Start of Day option This is used to prime up the instrument with Diluent usually PBS Follow the screen prompts Whilst the instrument is priming check that the syringe on the left hand side of the instrument is filling with diluent and that there are no large air bubbles present The instrument is now ready to run 6 2 Loading the RunTime Software Load the Runtime software by selecting the Runtime32 icon on the desktop This will load the software that drives the Xsp 220 The arm and syringe shou
21. the files by type 5 Find and highlight all the Area files A02 6 Right click on the mouse and chose the Send to a USB stick or CD ROM 7 Write the files to the CD Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 6 1 Liquid Level Following When aspirating liquid from a container the probe will attempt to follow or track the liquid surface as it goes down The probe will travel down in a number of discreet steps The number and size of these steps will depend on 1 The Aspiration Volume The larger the volume the greater the number of steps 2 The Effective Diameter The smaller the diameter the greater the number of steps 3 The Insertion Depth The greater the specified insertion depth the fewer the number of steps If aspirating a small volume eg 10 ul from a tube of 10mm internal diameter and with an insertion depth of 1mm the probe will not need to move down beyond the initial insertion to perform the aspiration The level change will be less than 0 2mm However if aspirating 200 ul the liquid level will fall by approximately 2 5mm so it will be necessary for the probe to move down to ensure the needle remains submerged t Insertion Depth after first part of aspiration Insertion Depth 4mm Insertion Depth 4mm 1 mm Effective Diameter in mm Minimum Liquid Height LARGE VOLUME ASPIRATION 1 2 3 Figure 44 Liquid Level Following during large volume aspiration
22. the ones currently defined for your system You can do this using the SETAREA program Select one of the more critical Areas such as a Microplate or a Slide Area that has small wells and send the probe to the Clearance Height of the first well If the position of the new probe appears to be offset slightly you can do one of the following e Redefine the co ordinates of every Area on your system This is not quite as bad as it sounds as you can probably just apply a nudge factor in either the X or Y co ordinates to each of the Areas e Physically move the rack locators to the left or right as appropriate for all the affected racks along the locating rails until the rack is in the correct position This only works if the probe is out of alignment in the X as Y adjustment is not possible using this method e Bend the probe into the correct position This is the fastest method but take care not to over bend the probes Note that probes are considered to be a consumable not covered by the standard warranty Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual The length of the new probe should be within 0 5mm of the old one so for most Areas the Z co ordinates should not need to be adjusted However for critical Areas such as the disposable tip racks and microplates if the height of the new probe needs modifying it is best to do this by adjusting the Z co ordinates for each of the affected files This can usually be done
23. try to run the applications software If it is you will need to copy the Area file from the kwinsp defaultareas directory into the kwinsp directory Runtime System Error Failed to evaluate an expression Bad Syntax in the following formula 1 This arises when trying to run some Applications with the wrong Regional Number Settings Failed to communicate with instrument Switching to Off Line mode Instrument is not connected to computer or it is powered off Incorrect Port setting in configuration Z Error Occurs when probe has been unable to reach the destination programmed This may be because of an obstruction Can occur if Search Height is set too low in the Tip rack Area 7 3 Error Logs A number of log files are created to record specific errors if generated during processing Make sure that the logging option is selected in the Configuration program See 4 6 7 The log files are Beedrive log Runtime32 log SetArea32 log Runtime log If you encounter an error during processing your supplier may ask you for a copy of these files They are all located in the C kwinsp directory You can locate and group them together using Windows Explorer and by clicking on the Type tab on the top right hand screen and looking for Text files The most useful is the Beedrive log which is overwritten each time the Runtime software is loaded If you do encounter an error during processing you must make a copy of this file before running t
24. will make every effort to remove these substances from our products but currently our products fall under the category of Monitoring and Control Instruments and as such are not included in the directive Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 13 Warranty l HTZ Ltd Warrants to the Purchaser for a period of twelve months if supplied direct to a Customer or fifteen months from initial shipment if supplied to a third party that the Company s instrument is free from defects in workmanship or material under normal use and service This warranty commences on the date of delivery to the Purchaser During the warranty period the Company agrees to repair or replace at its sole discretion without charge to the Purchaser any defective component The Purchaser must inform the Supplier of the instrument of any defects to arrange repair either by return or on site Repaired Instruments shall carry the same amount of outstanding warranty as from original purchase or ninety days which ever is the greater This warranty is contingent upon the Customer following the Daily Routine P 2Jand Regular Maintenance P procedures All parts listed as essential Spares and Consummables are excluded from the warranty Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual The recommended Bio hazard decontaminating agent is MedDis and is available from Hay Man Medichem 11 Westerham Road Bessels Green Sevenoaks
25. 1 HTZ Xsp 220 Operator Manual Probe Support Rod 6320 040 AH Support block Figure 9 Screw the connector of the larger diameter tubing into the left hand port viewed from the side of the syringe valve Figure 11 This is marked Small Probe Screw the connector of the smaller diameter tubing into the Pressure Sensor port on the back panel See Figure 10 Insert the Probe Support into the Support Block Adjust the position of the sleeve along the probe so that the probe is supported as shown in Figure 4 Clip the probe tubing into the loop of the Probe Support which should be bending towards the front of the instrument Probe Syringe Tubing Syringe RS232 cable 7 2020 009 Probe Pressure o Sensor Tubing Sensor Po Figure 10 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 6 2 Modes of operation There are two methods of operation with the XSP 220 DT and this affects the number of tubing connections required When operating in the Wet mode the syringe and probe are primed with distilled water At various intervals during processing the probe will pipette a small volume of liquid into a washbowl if fitted or a container A system fluid bottle supplies the syringe with distilled water When operating in Dry mode the syringe and probe tubing are left empty This simplifies the processing and removes the requirement for system liquid associated bottle Washbowl and waste c
26. 1 mm above the bottom of the tube to prevent it from being completely blocked in this situation Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Figure 66 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 3 5 Disposable tip blow out volume This field controls the amount of air used to blow the source liquid out of the tip to ensure complete ejection of the volume aspirated If using Wet mode 50ul is usually sufficient If Dry then use a value of 100ul enema Eater ihe add iesel vole in bie the arms pe al a dispar tip Figure 67 8 4 3 6 Flush volume The Flush volume resets the liquid column in the probe when using the Wet mode This prevents any bubbles from breaking up during processing which may cause a change in the performance The volume specified here is dispensed out of the probe in between each sample and is typically set to around 200ul If using Dry mode this should be set to 0 in which case the probe will not visit the Washbowl except in certain error conditions Enter the feeh moienn jmicaltines iebvoen samples in rectora the quid cole Figure 68 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 3 7 Source Position flag This controls the sequence of processing in the Source tube rack Tf set to O the tubes will be processed strictly in the position sequence in which they occur in the rack eg 1 2 3 4 5 etc If set to 1 the sequence is controlled b
27. 2 laser product as defined in IEC 825 1 1993 The radiation is in the wavelength range 400 to 700 nm and eye protection is normally afforded by aversion responses including the blink reflex Background colour green Symbol black le Meaning Earth ground terminal IEC 417 No 5017 Background colour green Symbol and outline black Meaning Protective conductor terminal IEC 417 No 5019 MN Alternating current the frequency Hz voltage V and current A or power consumption W or VA will be specified IEC 417 No 5032 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 2 Unpacking the instrument The instrument is shipped in a cardboard shipping case which has been designed to minimise the possibility of damage during transit However when unpacking the instrument you should check that there is no obvious damage to this box or any of the contents If there is any damage you must inform your supplier and relevant shipping agent immediately 3 3 Packing List A packing list Part 9967 700 is included with the instrument that is specific to the configuration of instrument Please check this carefully to ensure that you have located everything within the box before throwing it away There are some small components that may have fallen to the bottom of the containers they are shipped in When removing the instrument from the cardboard case do not lift the instrument by the arm This c
28. 4 6 3 Hardware 4 6 3 1 Instrument OnLine You will be prompted as shown in Figure 20 to specify whether the instrument is Online or Offline This prompt is the same as is also displayed if you select the Hardware menu option If you want to drive the Xsp 220 you should check this box If you always want to run the PC in simulation mode useful for checking Protocols without driving the machine you should uncheck this option If the option is checked the software can still be run in offline mode simply by switching the machine off BEFORE running the software although it takes a few seconds to establish the instrument is not present Pue Figure 20 Instrument Online prompt Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 6 3 2 Port parameters for Serial Port Enter the number of the Serial Port of your PC that is being used to drive the instrument For desktop computers this is usually Port 1 but it does depend on the computer For example many laptops are set at Port 3 Remember which port you have selected and if the PC has more than one make sure the serial port cable supplied is plugged into the correct one meterem Em Cok Peete Puse Figure 21 4 6 3 3 Port parameters for USB Port If your computer does not have an RS232 port you will need to use a USB to RS232 converter Part These plug into a USB port on the computer but terminate with an RS232 connector The Com Port that your PC allocates to th
29. RNING If the cover is removed for any reason Electrical Mechanical Hazards can be exposed it is therefore IMPORTANT that the power cable is REMOVED prior to gaining access to any internal components 12 2 Environment The XSP 220 is suitable for use with an ambient temperature range of 18 C to 31 C with a maximum relative humidity of 80 for temperatures up to 31 C 12 3 WEEE Directive Directive 2002 96 EC The Waste of Electrical and Electronic Equipment directive has been introduced to minimise the quantity of electronic equipment entering the landfill waste stream and to ensure the proper disposal of such equipment HTZ complies with this directive and will accept the return of any equipment sold by us after August 2005 All other instruments can be returned only with the agreement of HTZ Ltd To return product please contact HTZ Ltd for a RCN number Return shipments are the responsibility of the customer Where necessary instruments returned MUST have a decontamination certificate HTZ retain the right to refuse returns where this is in doubt e This only applies to England Scotland Wales and Northern Ireland 12 4 RoHS Directive 2002 95 EC The Restriction of Hazardous Substances directive is aimed at restricting or eliminating the use of lead mercury cadmium hexavalent chromium polybrominated biphenyls or polybrominated diphenyl ethers in electrical and electronic equipment HTZ Ltd together with our suppliers
30. TES WASHBOWL The washbowl Used for washing the probe when The Washbowl Area Type allows for two operating in Wet mode positions to be entered but the Xsp 220 is fitted with a single position Washbowl therefore use the same co ordinate for both positions TIP1000 Rack for disposable Uses to locate either boxed or tips refill type tips DTSAMPLES 96 position tube rack Primary Sample tube rack DTDEEPWELL Rack for a deep well For microplate or other SBS plate format item including deep well storage plates TIPOFF Waste Tip Bag Support Collecting used tips Usually a single position only DTTESTPOT 150ml container or Test Area used for checking Used to check the Z Motion is operating similar operation of instrument correctly Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 3 Pre defined Area Files When the files on the Configuration Disk are installed a set of pre defined Area files will be copied into the main software directory C kwinsp These are the files used when the instrument was tested in the factory and are specific to your instrument It is not essential to use them but it can save some time and it is recommended that you use them initially The co ordinates in these Area files need to be checked and adjusted before operation in case of any small changes that may have occurred to the instrument during shipping 5 4 To edit a pre defined Area file 1 Double click on the SETAREA shortcut already installed
31. Tapered tubes WARNING When the internal diameter of a tube is smaller at the bottom than the top of the tube it is important to check that the probe remains beneath the liquid surface during the entire aspiration step whatever the initial liquid level This can be checked using the Utilities Function Checks Liquid Following Test 5 7 Volume Calculation VOLUME CALCULATION The volume of liquid in a tube is calculated as follows Volume of a cylinder 7 r h Where r Effective Diameter 2 and h Calculated Depth and Calculated Depth Liquid Height Insertion Depth Many tubes have a larger diameter at the top compared to the bottom It is Insertion Depth mm important to enter a value for the Effective Diameter which is representative of the tube If in doubt alculated Volume use a smaller diameter This will help to ensure that the probe needles will remain under the surface of liquid during the aspiration Liquid Height Calculated Depth Effective Diameter as specified in Area File mm lt Minimum Liquid Height Dead Volume Figure 45 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 8 Area Definition Terminology The following is a glossary of some of the terminology specific to the Area Definition process TERM DESCRIPTION Area An item on the bed which needs to be accessed by the probe e g a rack reagent bottle or the washbowl
32. Xsp 220 DT Operator Manual Document 9967 901 Issue 1 TABLE OF CONTENTS 11 INTRODUCTION Nn A 1 1 Basic Overview of the Xsp 220 DT 1 2 About this manual 1 3 Summary of steps involved in setting up and using the instrument Nn E E en __ 9 y yo E o a 5 a i ya n NO 3 5 Levelling the instrument 12 4 1 Computer Minimum Specification 22 4 2 Software Overview 22 4 3 Visprog Installation New Installation N N eel Y 4 7 Updating Software 33 4 8 Operating System Settings 33 Document 9967 901 Issue 1 Windows XP Professional 4 8 WANCO WS VISTA 2 5 ow cates NAN e tn WN Barat persicae O A T AS 4 9 Dongle installation USB 34 4 10 Switching on for the first time 34 n o z o 3 zZ we 6 2 Loading the RunTime Software 52 6 3 Priming Wet Mode 53 6 4 Pre run checks 53 7 1 Liquid Handling Fault Finding table 60 7 2 Software Error Messages 62 7 3 Error Logs 62 8 1 Overview 63 Document 9967 901 Issue 1 8 2 Configuration Main Menu 63 3 A A 8 5 Data Configuration 75 0 3 ile Css vasa devs cubes A A EL REEL baba TOU Uses E A EE a ade Re 8 6 Saving Changes 79 We 00 A 11 1 Instrument dimensions 1 2 Power requirements 11 3 Chemical Compatibility 8 00 A 12 REGULATORY ISSUES 2 1 Declaration 2 2 Environment ooj oo fj ua 12 3 WEEE Directive Directive
33. ans of the optical Tip Detector If the Tip rack is not installed correctly and the instrument is not able to move down smoothly it may detect a Z error It will retry the move 5 times before finally displaying a warning allowing you to correct the fault or abort If the Area file has been badly defined you will need to abandon the run redefine the Area and restart If the instrument runs out of tips during processing the operator will be prompted to load more tips 6 6 3 Interrupting Processing You may interrupt processing at any time by pressing the STOP button on the screen or the Pause button on the keyboard Pressing the lt F10 gt key also works 6 6 4 Screen Display During processing a large amount of information is available about the processing The main screen displays a graphical representation of the deck showing which positions have been processed by means of coloured circles If the position has been processed successfully it will show a green circle If any errors were encountered it will display a different colour depending on the error e g a red circle if insufficient liquid was detected in the tube In addition to the graphical display there are also a number of tabs at the bottom of the screen that record information in a tabular form throughout the processing The tabs include Run Details Basic information on length of time processing the batch Liquid handling failures Records any liquid handling failures Activity
34. ashing into any object 5 4 2 1 Checking the pre defined X Y coordinates for the tip rack Limasions Curent Loratien ote Locator Wie Treanna Higi Wis Termene Heighi Shears Hesgbi Emi of Fisi Flor Y End of Laat Fens H Ci spera es Herb Y End of Lost Fep Y L Dingana n Heiki T Hin Liquid Haighi Figure 36 You will see that for the TIP1000 Area there are 3 pairs of X Y coordinates namely Start of First Row X and Y End of First Row X and Y End of Last Row X and Y A rectangular array such as the Tip rack the Samples rack and the Deep Well rack can all be defined by means of 3 X Y coordinates You will see that the Location is defined as having 8 positions per row and 12 rows Also notice in the example above that the Y coordinate for the End of First Row is greater 565 than that for the Start of First Row 61 This means that the Start of First Row has been defined towards the back of the instrument Remember that the Y home position Y 0 is when the probe is at the back Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Start of First Row X 1728 Y 61 End of First Row X 1727 Y 565 End of LastRow X 2524 Y 570 YY E Figure 37 When defining the tip rack you should do this with tips in the 3 positions as shown in Select the Start of First Row X field using the mouse cursor Drive the probe to the position using the Probe to Current XY The probe should be c
35. creating sample ID This controls how the sample identifiers will be created If set to 0 it will create a series of sequential numbers You will be prompted to enter a starting number called a seed and the rest of the series will be created by adding to it If set to 1 you will be prompted to enter a barcode which can be achieved by means of a Wedge reader or keyboard entry You will not be prompted to enter codes if using an existing Worklist ion of creating sampe II Enter augeenital sumbar barcode coset nr 1 arimea Figure 75 8 4 3 15 Record actual volume transferred This controls whether the volume transferred is recorded in the Worklist or not Enter 0 not to record the volume Enter 1 to record the volume that was actually pipetted If the volume is less than the volume that was specified it will record the volume that it has calculated was in the tube as this is also what it will attempt to transfer Note that as most tubes especially plastic ones are tapered and the calculation assumes a straight walled cylinder this will only be approximate Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Keene actua valur brorberned Y C al Ember gt du mui ing 8 gt record tho ecizal mojumo depend i Hia werkt Figure 76 8 44 LoadSamples Type Parameters The Load Samples type application is used only for the creation of a Worklist containing sample identifiers Curently there is only a sin
36. e button Figure 40 You can then define the X Y coordinates for each location Note that th Z coordinates are common for all locations within an Area as it assumes the locations are all at the same height Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 4 8 WASHBOWL X Y Co ordinates The Washbowl is usually only used by the Xsp 220 when the instrument is operating in Wet mode Only 1 position is required when defining the Washbowl Therefore to set up the area needed move the cursor over to the first field Flush Position X and click it Move the probe to the centre of the Washbowl using the Virtual Joystick and press the Fix button This will record the co ordinate for both the X and Y simultaneously and the cursor will move onto the next field named Rinse Position X Press the Fix button again to record the same co ordinate Save the data File gt Save Com rales muri ba dabned wth al szad Posen Figure 41 5 4 9 TIPOFF The TIPOFF Area is defined as a special Area Format called Tipoff It only requires a single X Y coordinate which should defined immediately above the waste bag No Z coordinates are required see Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Figure 42 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 5 Nudge Co ordinates This option is designed to allow you to apply a change to all of the X and Y co ordinates in an Area It is particularly usefu
37. e using Currently the Application uses only the Areas specified in the Liquid Handling 1 Source and Target fields To change the Areas used select the Change button and navigate to the Area file you require Figure 61 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 2 Application Type When creating a new application by using the Add After button you will be asked to specify the Application Type There are currently 2 Application types namely the Transfer Type and the LoadSamples Type To select a differentType press the Arrow keys Figure 62 Having selected a Type you will be prompted to give it a name You will then be able to edit the parameters for your new application To save a new application choose Exit followed by Save and Exit Note that if you have not changed any parameters from their initial default the application will not be saved 8 4 3 Transfer Type Parameters 8 4 3 1 Volume specification method This specifies where the volume to be pipetted is controlled Enter 0 to specify within the application avoids the user having to enter it each time but cannot be changed Enter 1 to allow user to specify volume when application is run Enter 2 to read the volume from a worklist This would allow a different volume to be pipetted for each sample Enter your choice and select the right arrow button to move onto the next parameter Volume sgecdicaisan mind F gt Ember volume m fod e
38. ed For a full guide on where the heights should be defined for the different Areas refer to Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 4 3 1 Defining the Heights for the tip rack TIP1000 The bottom left section of the Area Definition window shows the Height Data To enter height data for a specific data point first make sure the cursor is over the relevant field and click it The current number will shift from the left to the right hand side of the field and this shows it is ready to receive data from the Virtual Joystick For the TIP1000 Area set the Min Traverse and Min Clearance Heights to 1 You can do this just by typing in the number into both fields In fact you can enter any coordinate this way The Search Height is the height used for picking up the tip and this is critical Set the Search Height to a point where the tip adaptor is inserted into a tip and the spring is compressed by approximately 50 of its original length If you compress the spring too much you may get Z errors during the Definition process but also during operation If you do not insert the tip far enough the tip adaptor may not seal properly and you could get dripping from the tip and could possibly fall off during a pipetting process When the Probe needle is at the correct height press the Fix button on the Virtual Joystick This records the co ordinate into the current field and moves it onto the next field If you want to skip a field si
39. elow the Source ID column is the first and Source position is the second A total of 7 fields has been specified de seats Lil pare fi it J rt bd kd kal Sal 2 0 800 Figure 83 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual These fields have the following fixed meanings Field Meaning Source Rack ID The identifier of a source rack usually a linear barcode read by the instrument Source Tube Position The position within a given source rack whose tube is to be processed Source Tube ID The identifier of the source tube typically either a 2D bar code or a linear bar code Target Rack ID The identifier of a target rack usually a linear barcode read by the instrument Target Tube Position The position within a given target rack whose tube is to be processed Target Tube ID The identifier of the target tube typically either a 2D bar code or a linear bar code Dissolution Volume The volume of diluent microlitres to be dispensed Mixing Volume The volume of liquid to be aspirated from and dispensed back into a tube during each individual mix step Mixes The number of mix steps to be performed on each tube Maximum Aspiration Volume The maximum volume to be aspirated from a source tube when transferring liquid to a target tube Transfer Volume The volume of liquid to be transferred to the specified targe
40. entral with respect to the tip If you find that the black tip adaptor is not central in the tip then move the probe into the correct position using the Virtual Joystick Note that although there is a certain amount of side to side movement in the tip adaptor and Z rack you should ensure that its average position is in the centre of the tip Having achieved that press the Fix button to overwrite the existing coordinate If no change is noted click the FIX button again to check fields have updated correctly The fields Start of First Row X and Start of First Row Y will now be updated to show the new values The cursor will also be moved automatically to point to the next field indicated You may use the Probe to Current XY to physically move the probe to this position Repeat for the process the remaining positions i e End of First Row and End of Last Row 5 4 3 Checking the pre defined Height Data Z Coordinates Here we are checking that the Z coordinates for a given Area are correct Y ou will see that there are 6 Height Data Parameters namely Min Traverse Height Min Clearance Height Search Height Dispense Height 1 Dispense Height 2 Min Liquid Height These control the height at which any are operations performed in this specific Area The names are only a guide as to how they are used and are not all relevant to all Areas For example the Area TIP1000 is used only for picking up tips and it is only the first 3 that are actually us
41. ftware such as Excel type spreadsheets as worklists you will need to ensure that the Regional and Language settings match those shown in Figure 32 Le they need to be set as UK English You must also ensure that the settings for the decimal symbol and digit grouping character are in the standard English Language format as shown below keglonal and Language Uptions Reganal Opaons Langqueges Advanced Standards snd dormais This option efectes how some progres tom numbers currenciss deies and tia Selaci an Ren da match fs preherenced or cick Lusiomine to Choose your Own tarnete ha Customze Semples Number 123456 789 00 Cumenge 6123 46 299 00 Time 15 10 24 Shar date oem Long daie 02 Februney 2007 Locator To help serices provide you iih local intermaton such as naws and weather select ynur present location United Kingdom Figure 32 4 8 2 Windows Vista Not yet supported Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 9 Dongle installation USB The Applications Software is supplied with a security key or dongle Figure 33 This needs to be installed in the PC before the instrument will run the Application provided To install a USB security dongle simply plug it into any available USB port Windows will prompt with the message Found new hardware and will proceed to install the software for the device automatically You will not need a separate driver disk Once co
42. gle parameter to enter for this application The identifiers can be created either based on a sequential series or using a barcode reader as described already for the Transfer application B 4 3 14 Figure 77 To Save choose Save followed by Save and Exit There may be a long pause whilst it writes the files Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 5 Data Configuration The Data Configuration Menu is used to set up the parameters associated with the Worklist files Figure 78 8 5 1 File Type Selecting the File Type button will reveal the following window The application software works with either Microsoft Excel or Comma Separated Variable CSV formats If you wish to change the current setting click Change The Change File Type window will appear You may currently specify either Comma separated Variable or XLS as the file type to use Dimasa Sapani Yabahle Figure 79 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Figure 80 Click the button corresponding to the file format you wish to use Please note that 1f the Excel format is selected this will only work with files in Excel 95 format and without macros 8 5 2 File Locations 8 5 2 1 Worklists When an instrument application is run a worklist is selected from a pending folder During processing the worklist is transferred to a processing folder and on completion the worklist is transferred fro
43. hat the rack is sitting absolutely flat on the deck You may find that if the securing screws that hold the rack down onto the locators are too tight it may cause the rack to rock These screws only need to be finger tight E E y lt 0 4 mm variation in this gap across the rack Figure 16 3 Push a tip onto the probe and move the probe manually so that it is positioned just above the tip rack 4 Move the probe down so that the probe needle is within 0 2 mm approximately the thickness of 2 sheets of photocopier paper of the black top plate Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 Move the probe to different positions within the rack and observe any differences in the gap 6 Compare the distance between the probe and the top of the rack at the front and back and left and right hand side of the rack This is best done viewing from the side If the height difference is gt 0 4 mm the arm may require realigning or the rack itself may need to be adjusted You should consult your service engineer to perform this procedure before defining any areas ALWAYS MAKE SURE THE INSTRUMENT AND ARM ARE LEVEL BEFORE DEFINING ANY AREAS 3 8 Connecting to the power supply Having installed the racks and checked that the instrument is level you may now connect the power cord These instruments are fitted with a variable voltage power supply which must be fitted with correctly rated fuses to ensure maximum protecti
44. he Z home Traverse when moving into the Area i e the edge of washbowl adaptor set to a height above the top of above the top of coordinate and the Z Negative Height tallest part of the Area Note that or to Ifor approx 5mm above the the sample tubes the Deep well Home for ejection you should usually define this with maximum safety highest part of the rack or 1 plate or to 1 a tip fitted to the adaptor For Safety set to 1 Min The height the probe needs to be at Set to height that Set to a height approx As above As above N A Clearance when moving from one position to probe can move 5mm above the sample Height another within the Area For safety within washbowl tube within the rack this is usually the same as the Min Traverse Height Search The height the probe starts N A This is the Tip Pickup Set to the top of Set to 0 5mm N A Height searching for liquid from or if the Height the Dilution above bottom of level sensor is off the height from Set to a height approx tubes tube which it aspirates where the spring is compressed by approximately 50 60 O Dispense The height which the pipetting Set to same as N A N A Set approximately N A Height 1 needle dispenses from Clearance Height 2 or 3 mm into the top of the tube Dispense N A N A N A N A N A N A Height 2 Minimum The bottom of the tube i e where it N A N A Set about 1 mm Set about 0 5mm N A Liquid should stop looking for liquid Also above bottom of
45. he instrument again All of the log files are text documents and can be opened in Notepad Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 Creating New Applications 8 1 Overview The XSP 220 is provided with two programmable applications used for transferring samples and creating worklists Each of these can be configured to meet your specific requirements by modifying a number of parameters using the Configuration Application This also allows a number of Hardware and Data options to be configured Note that this not the same program as the Instrument Configuration program described earlier in section 8 2 Configuration Main Menu From the Runtime Main Menu select the Protocols gt XSP 220 Configuration option You will now have options as shown in The Hardware options allows you to configure certain global parameters that will affect all applications The Applications option allows you to edit and create new Applications The Data option allows you to configure parameters associated with the file handling e g worklists Figure 56 8 3 Hardware Selecting the Hardware button will display the following options shownin Figure 54 Figure 57 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 3 1 Probe Insertion depth The Probe Insertion Depth controls how deep the probe will insert under the surface of liquid once it has detected liquid Setting this value will ensure that the wetting
46. in The speed will now have reduced Take particular care when approaching a rack that is higher than the current height of the probe You should make sure that the probe is moving in small increments Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 4 2 Checking the pre defined X Y Coordinates You can drive the instrument to any position on the deck using the arrow buttons However when editing a pre defined Area you can also move the probe directly in a single move to a specific coordinate This is useful when you want to quickly check the coordinates that have already been defined 1 Move the cursor onto the Coordinate that you want to verify The number in the field will align to the right when selected 2 From the Area Definition Menu Bar select Probe gt To Current XY The probe will move to that position but will remain at the Minimum Traverse Height which is usually set at a value of 1 To make a precise check you will need to move the probe down You can do this either with the Z Down Arrow on the Virtual Joystick OR you can highlight a Z Coordinate in the Height Data pane and select the Probe gt To Current Z To move the probe down press on the Z Down arrow on the bottom right hand corner of the Virtual Joystick Be patient the acceleration in the Z is slow but be cautious as well as it does accelerate eventually Again if in doubt go slowly and release the mouse button well before the probe is in danger of cr
47. in the worklist for processing later ID of tube 1 110001 OS Estat i on o 0 de ah a et I iei Figure 54 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual The resulting worklist would look like Figure 54 with the SourceID column populated with numbers starting with the number you entered as the Seed E Ce es poat Pyrat Tos Gate ido toe Ao E UE E JE Cl e i are O Bemir Pres Tegel E Tepi Pride veers Arcis oes TETI fn PE 1 i re 3 POH amy t Peura 3 mu i Pera ne E PEHEE iE i Ci Pitas ur J Pearl TN E PENA Lund B PEE pd iD Pira tir n uirai LE iz Pe Ho wiy 17 FEA Uii til HAG Tr iE Eura E ip Feu do i PEH ono ii FE Dara Te i PE uma TOJI la PEWS SS SSS SESS CEE RE Pee NN VULVA cara UA dd e Figure 55 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 6 6 Processing Started 6 6 1 Startup Once all the parameters required by the application have been entered the instrument the arm and the syringe will go home if they have not already done so If there is a tip on the probe it will be ejected into the Waste Tip Bag The syringe will also prime itself if required 6 6 2 Tip loading The probe will move to the Tip rack and attempt to pickup a tip from position 1 If none is found it will automatically move to the next position in the rack and try again until it locates a tip The instrument checks that a tip has been loaded by me
48. is device can be anywhere between 1 and 15 Check in Windows by looking in the Start gt Control panel gt System gt Hardware gt DeviceManager gt Ports Com amp LPT The example below shows COM10 in which case this number should be used as the Com Port number in the Port Parameters field above Eads 4 y Mow ard other parang deca Bites abo E J POMC sipin Por Caio Daria a dei Inver Sa Ea cori Figure 22 4 6 3 4 Barcode reader The tabs labelled Bar Code Reader Stirrer Arm and Incubator are not currently applicable to the Xsp 220 and you should check that they are configured as not being present The barcode reader option refers to an integrated barcode reader mounted on the arm Note that if you have a wedge type manual barcode reader it is not necessary to configure this in the HTZ Configuration program as it behaves like an additional keyboard Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 6 3 5 Pump The tab labelled Pump defaults to a 1000 ul syringe volume Make sure that this matches what has been supplied with the instrument The DT machine may be supplied with a 2500ul syringe ua Poet Peers Boda Bam Eura 1 ie I Puea Type bemi re 4 6 3 6 Security Cover If you have a Security Cover but do not want the software interlock to operate make sure you choose the second option The No Cover option should not be used if you have a cover fitted as the addi
49. king liquid surface correctly remains under surface of liquid As probe is not moving fast enough the throughout the aspirate step No error generated probe ends up aspirating air Pippeting code on screen Green Q Pippeting code in Activity Log OK Appeared to perform the aspirate step OK 17 Aspirate speed set too high not giving probe Check value for Aspirate speed in the enough time for aspirated liquid to enter tip protocol This value should be 50 18 Froth on top of diluted sample causing probe to aspirate air 6 Bubbles on top of samples 19 Dispense speed too high Reduce dispense speed and introduce mixing step to achieve homogeneity 20 Dispense height too high Lower Dispense Height for the Area Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual file 7 Droplets on tip of probe 22 Syringe tip is worn Change the syringe tip 23 Syringe connectors not tight or 1 of the Check that the white teflon washer is teflon washers is missing present in those ports which require one 24 Syringe valve is leaking rare on new Replace valve instruments 8 Washbowl flooding 25 Waste tubing has a kink or loop in it or Ensure that the gravity waste tubing bottle is full can drain freely into a waste bottle Ensure that the tubing runs downhill all the way to the mouth of the bottle 7 2 Software Error Messages Area not found One of the Area files is missing when you
50. l if you are trying to modify the position of the probe within an Area which has a number of locations It allows you to modify all locations by the same amount in either the X or Y direction The Nudge Co ordinates option is available from the Area Definition Edit menu The nudge can be applied to either the X or the Y co ordinates and you can select whether it is just the Current Rack or all Racks within the Area that are modified r n rarsban of ste 200 te 110 Shad af Tari Pee bet of rl Flee Y Sie Tiare Hill fet of Feat Hor A Clean rece Inept Tei al fei leet Seach Hosp Sedo Lal Hom H ape Haga 1 Ar of Lait Aen Y Guper lea 7 Ein eget Ferrey Figure 43 5 6 Backing up Area Files Once defined and checked the Area files must be backed up onto some removable media such as a CD ROM It is also useful to make a copy in a safe directory on the PC so that they are readily available The Area files that need to be backed up are the ones used by the installation and that have been defined for your machine These are located in the Kwinsp directory These should not be confused with the Default Area files that are located in the Kwinsp DefaultAreas which are effectively for reference only To back up your Area files 1 Insert a memory stick or blank disk into a USB or CD drive of the PC 2 Load Explorer or My Computer 3 Locate the C Kwinsp directory and double click on the directory 4 Click on the Type field to sort out
51. ld home themselves at this point if they are not already there Note that the syringe may take several seconds to home itself if the instrument has previously been switched off with the syringe at the bottom of its stroke Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual e Gmm immi timing Ps ia Ti Pci ll a finden errs Figure 47 You will now be able to run a Protocol by selecting it from the Protocols menu shown at the top left of the screen 6 3 Priming Wet Mode At the beginning of the day you should run the Prime Option This will ensure that the syringe and probe are fully primed with System Fluid It is not necessary to prime if using Dry Mode 6 4 Pre run checks Whenever running the instrument you should check that the System Fluid Bottle has been filled Wet mode only Racks are all located properly and pushed down onto their locators New tips are loaded Gravity waste bottle is empty Wet mode only 6 5 Running an Application To run an Application from the Initial Runtime screen Figure 47 Runtime gt Protocols menu Select the XSP 220 Applications option You should now have a menu that looks like Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Archiving ik relat iad Samples Liad Dampies Geparridal Figure 48 Additional applications options may also be displayed All the applications provided are associated with the pipetting of samples from a s
52. led Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 11 Technical Specification 11 1 Instrument dimensions Width 475mm Depth 565mm Height 350mm excluding probe 700mm vertical clearance required to allow for free probe movement 100mm clearance at rear required for cables Working Area of Probe without cover 330mm X x 275mm Y x 105mm 2 Working Area of Probe with security cover 328mm X x 275mm Y x 105mm 2 Resolution of stepper motors X 8 steps mm Y 8 steps mm Z 22 5 steps Syringe Drive 5000steps 60mm Weight 19 5Kg approx 11 2 Power requirements Supply voltage 90 V AC to 265 V AC Power consumption Maximum 200VA 11 3 Chemical Compatibility Fluid path Syringe Glass barrel and PTFE seal Syringe valve Fluoroelastomer seal Probe Needles PTFE coated stainless steel Probe tubing FEP amp PTFE System fluid tubing HDPE Tip Adaptor PCTFE All valves Syringes amp tubing are manufactured from glass Kel F PTFE LDPE amp FEP based materials for chemical resistance except to HF amp HF compounds Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 12 Regulatory Issues 12 1 Declaration This instrument complies with the appropriate Council Directives pertaining to EMC Electrical amp Mechanical Safety a copy of our DECLARATION of CONFORMITY detailing which directives are covered can be ordered separately The Xsp 220 has been CE marked WA
53. lowing parameters also to be entered 5 4 6 1 Usage Must be set to Normal as the other options do not apply to the Xsp 220 5 4 6 2 Area Probe Must be set to 1 as there is only one probe 5 4 6 3 Effective Diameter This is the internal diameter of the tube or container You should take care when setting this parameter for containers in which the probe will perform an aspiration step It should be set to the internal diameter of the tube or bottle where the liquid could be held This figure is used to calculate what volume of liquid is in the container The volume calculation is also dependent on the height at which liquid is found and the Minimum Liquid Height This parameter also determines how fast the probe tracks down during aspiration The smaller the diameter the further down it will travel for a given aspiration volume In practice you may have to set the diameter a little smaller or larger than it actually is However it is most important that whatever you set it to you check the probe remains in liquid during any aspiration step from the container Running a dummy assay can usually do this Note This parameter is entered in millimetres Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual GENERAL WASHBOWL TIP1000 DTSAMPLES DIDEEPWELL TIPOFF Min The height the probe needs to be at Set to clear top With a tip fitted to te Set about 5 mm Set to 2 mm N A only uses t
54. lways install the Visprog2 before installing the Application Disk 1 Insert the disk labelled Application Disk CD into the CD drive of the computer 2 Locate and run the Set up program located on the CD by selecting the Windows Start button Start Run and Browse located in the root directory of the CD Follow the screen prompts pressing the Next Button when prompted 3 Accept the default location for files and folders 4 Enter your name and the name of the organisation if prompted to do so 4 4 2 File locations The DT Applications software also installs a number of files into the CAKWINSP directory It also creates a directory structure C XSP for the reading and processing of worklists It also installs a directory Kwinsp DefaultAreas that contains a set of default Area files Rack coordinate files These are examples and are not instrument specific You may either create instrument specific Area files using the SETAREA32 program or you can copy those created at the factory 4 5 Installing the Configuration Disk An additional diskette or CD is supplied which contains a number of Area files that have been defined with your specific instrument during its testing If this is the first installation of the software these files should be copied into the IKWINSP directory using Explorer Do not perform this step if the Area files in the kwinsp directory have already been edited to match your machine This diskette will not necessarily
55. m there to a processed folder These 3 folder may be different two of them may be the same or all 3 may be the same To specify these folders click File Locations on the Data Configuration menu The File Folders window will appear Mods ls ley presse Figure 81 To change any one of the folders click the appropriate Change button A Select Folder dialog will appear Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Leos E Faire Ti Cimer TET Bhi Pogai kee ar eee a a f AT HI oS aa iia fit _ 7 l pen meh Figure 82 Navigate to the folder required and select a file any file will do in that folder and then click Open You will return to the Folders window with the changed folder denoted Note that for this to work there needs to be a file even a totally blank one in the folder for you to select 8 5 2 2 Log Files and Layout Files Here you may also specify the path for the log files and the layout files 8 5 3 Worklist Format Click Worklist Format on the Data Configuration Menu The Worklist Format dialog will appear This dialog allows you to specify the format used by the worklists be they Excel or CSV files The left hand column gives the name of the fields as used internally by the XSP 220 software and cannot be changed The names actually used in the Worklists can be specified in the Field Name column The Column No controls the order of the fields in the worklist and in the example b
56. maller diameter that is integrated within the probe called the Probe Sensor Tubing The sensitivity of liquid level detection is enhanced by a slow downward movement of the piston during the Search for Liquid move 1 2 About this manual This manual provides details of how to install and operate the Xsp 220 It also includes an overview of how to create the programs called protocols which run the Xsp 220 However more detailed information is provided within the applications software itself in the form of on line help windows Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 1 3 Summary of steps involved in setting up and using the instrument The following is an overview of the steps involved in setting up and using the instrument Unpacking Installing probe racks tubing etc Check that instrument and arm are level and adjust if necessary Install Software onto PC VisProg2 Applications Configuration disk Defining Area files Creating an Application Running the Xsp 220 Sy On as EE Your supplier will probably have already performed the majority of the set up procedures so the initial sections may not be relevant to you other than as a reference In addition to sections on the above this manual also includes additional reference sections on maintenance fault finding and information on spare parts For full servicing information please refer to the separate Service Manual especially if adjustments are required
57. meters which control the execution of an IFA or ELISA assay Rack An item or container that sits on the Working Area of the Xsp 220 System Fluid The liquid that is primed through the Syringe and Probe and which is used for washing the Probe Normally distilled water System Fluid The glass bottle that supplies the syringe with System Fluid wash fluid Filled with Bottle System Fluid PBS or Distilled water Referred to as the Diluent Bottle in previous version of this manual VisProg2 The System software used for the creation and running of the IFA ELISA Application Waste Bottle Glass bottle which collects the waste from the Aspirate Needle Working Area The area of the Tray that is accessible by the probe Worklist A file containing information specific for a single batch which specifies which samples in a rack are to be processed for each Test Z Rack The toothed geared metal rod into which the Probe is inserted and which is moved up and down Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 Hardware Installation 3 1 Applied Markings One or more of these labels may be used on your instrument for health and safety reasons To enable their interpretation correctly each is explained Background colour yellow N Symbol and outline black Meaning Caution refer to accompanying documentation Background colour yellow AM Symbol and outline black Meaning Laser radiation do not stare into the beam Class
58. mple or 8 Minimum liquid height in Area definition set Redefine the Area and set the Reagent error but plenty of too high minimum liquid height to the bottom liquid present in container of the tube Although liquid surface detected it has Probe immerses into liquid surface calculated that there is Insufficient Volume by 1 to 2mm i e by normal IV to perform the aspiration step insertion depth Pipetting code on screen Red O Pipetting code in Activity Log Dry or insufficient 10 Effective Diameter parameter in the Area Redefine the Area and set the definition is set too small Effective Diameter closer to the actual diameter of the container Although liquid surface detected it has calculated that there is insufficient liquid to perform the aspiration step 11 Level detector sensitivity setting is Increase sensitivity borderline and not triggering reliably Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 Probe stopped before 12 Sensitivity may be set too high and the Reduce sensitivity of level detector reaching liquid surface surface liquid on the needle is triggering the during a search move level detector No error generated Pipetting code on screen Green Pipetting code in Activity Log OK Probe aspirates air as it thinks it has found liquid Subsequently fails to dispense correct volume 4 Probe stopped before 13 Probe has detected liq
59. mpleted you may need to reboot the computer to complete the installation The dongle is in effect the software license and is very valuable A replacement will be charged at the full list price of the software so it is recommended that it is secured to the computer with a security cable as some may assume that it is a memory stick and remove it Figure 33 4 10 Switching on for the first time The power switch is located at the rear right of the instrument As soon as the instrument is switched on all motors X Y Z and pump will be energised Depending on the version of firmware installed you instrument may Home itself This refers to the process whereby all motions return to their O reference coordinate The Home position is set at a position where the arm is at the extreme left hand side X 0 the Z Rack is at the rear of the arm Y 0 and the Z rack is in the up position Z 0 The syringe will also drive to the top position If the instrument sounds as if the motors are struggling to drive one of the motions home and continually drive then switch the power off immediately This can happen with the syringe drive if it has been driven manually or pushed up above its home position in transit With the power still off move the piston down manually by pushing on the drive plate and switch on again If the problem persists consult your service engineer Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 Defining the Areas
60. mply click the next field that is required with the cursor before pressing the Fix button A certain amount of trial and error may be required to get the optimum setting for this parameter Set Search Height at a height where the spring is compressed by approximately 50 Figure 38 5 4 4 Saving the changes Having checked and or edited the coordinates in the TIP1000 Area you may now save them From the SETAREA Menu bar chose File gt Save If you want to create another copy or have a modified version then chose SaveAs and give the file a different name Note Area files are stored with a A02 extension to the filename in the C kwinsp folder on the host computer 5 4 5 Defining the X Y and Z Coordinates for other Areas The method just described can be used to check the coordinates for other the Areas Refer to Table 1 for the information about what Height to set the probe for each type of Area Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 5 4 6 Area Information The upper left section of the Area Definition window shows the Area Information The first field in this section specifies the Area Format The Area Format selection will modify the rest of the display as the Area data required is dependent on the type of Area being defined For example the Washbowl Type requires relatively few data points or parameters other than the co ordinates Other Formats such as the DTSAMPLES may require one or two more of the fol
61. ne rubber tubing 7600 228 This is installed by pushing it onto the spigot that protrudes from the washbowl underneath the Deck Get someone to help you by lifting the instrument up at the front whilst you fir the tubing The other end should be inserted into a waste container such as a 5 litre flask Note that the waste system relies on gravity so the path of the tubing should be level or downwards but never upwards or you will get airlocks and the waste will not drain away correctly Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 7 Installing the racks Each of the racks is located on the bed using one or more rack locators These engage in a locating hole which is present on the bottom plate of the rack The locators are screwed onto the black rail that runs along the front and the back of the deck In the DT Applications the following 3 racks are normally supplied as a minimum RACK TYPE NORMAL LOCATION ON DECK Disposable tip Rack Block At rear left or right Tip Waste Bag Support Front left Sample Source rack Middle Destination rack Right hand side There are a number of variants on each of these rack types The precise type supplied will depend on the sample tubes and reagent containers you are using and the positioning on the deck will depend on the application When you take the instrument out of the box the locators will normally be fitted to the rail ready to locate your racks D
62. nstrument is being used for the first time Note that the colour of screen error codes where shown are the default values and may differ if the configuration has been customised Symptoms and Conditions Possible Causes Solution 1 Insufficient Sample or 1 Reagent error but plenty of Liquid level detector sensitivity setting is set Adjust i e increase the level liquid present in container too low sensitivity in the DT configuration program Probe immerses in liquid to a depth significantly more than specified by the application typically to the bottom of container i e failed to detect the liquid Pipetting code on screen Red O Pipetting code in Activity Log Dry or Insufficient 2 Pressure connector disconnected Connect it 3 Disposable tips are partially blocked or are Exchange tips for new ones being reused in a demo 4 Tip not picked up correctly because Search Redefine Height in tip rack Area not low enough 5 Search Height in Sample or Reagent Area Set Search Height higher to give time set very close to or below the liquid level for Piston to start moving before actually encountered probe encounters liquid 6 Tip adaptor badly scored and leaking Replace 7 Pressure sensor tube has become wet Remove Tip Adaptor and dry off This can happen if the system runs out of Purge pressure system with air using liquid when using Wet mode syringe provided 2 Insufficient Sa
63. ntered mi ran ime rad bom earidizd Figure 63 8 4 3 2 Default volume to be transferred Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual The application will default to pipetting the volume specified here If the previous option 8 4 3 1 is set to 1 the initial volume displayed by the prompt requesting the volume at Runtime will be set to this value Driesh rales ln ber E rater Tater the dalmal stos fito rallires in be irasan Figure 64 8 4 3 3 Liquid Sensing This controls how the level detector is used during the Sample Aspiration process Enter 0 to disable the level detector The probe would aspirate from the Search Height Enter 1 to use the level detector on each visit to an individual position Enter 2 to only use the level detector on the first visit to that tube Normally this should be set to 1 L el Ember nn manaig 1 gt aunen marp time 2 sense ancen in mach tube Figure 65 8 4 3 4 Action if insufficient volume This controls what happens if the volume calculated to be in the tube is less than the volume specified by the Default Volume Enter 0 to ignore the sample Enter 1 to pipette as much as possible In this case the probe will go down to the bottom of the tube Minimum Liquid Height and aspirate the Default Volume If the volume is actually less than that required some air will be introduced into the tip Note that the Minimum Liquid Height should always be at least
64. o not move the rack locators unless you know you have to e g when installing a different type of rack than the one s supplied with the instrument Always check that the probe can physically access all the positions in all of your racks before Defining Areas This is the process described later where all the coordinates are defined Rack locator 9972 218 Locating rail Figure 12 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 7 1 Installing the DT Rack The type of disposable tip rack supplied will depend on what types of tips you intend to use The rack will usually comprise one or more locations for either a box of tips or tip refills The rack must be located at the back of the instrument using the locking rack locators These have a washer and screw to allow the rack to be secured and to prevent it from being lifted by the instrument accidentally during operation The photos in Figure 13 bhow the rack before and after the rack has been secured DO NOT OVERTIGHTEN FINGER TIGHT IS ADEQUATE Figure 13 3 7 2 Installing the Waste Tip Bag Support Waste Tips are collected in an autoclavable bag which is supported by an adjustable support loop The base of this unit is fixed to the locating rail by means of 2 grubscrews This item will normally need to be fitted as it is not shipped in position It should be located at front left or back right location so that the highest part of the loop is nearest
65. of tip is minimised and will help accuracy reproducibility However if accuracy and precision performance is not too critical a larger insertion such as 3mm will allow the instrument to process more quickly Hi der ie phe a Hee Hip cal thes piale petrel je de tar jo rheesi Bajai Figure 58 8 3 2 Probe Sensitivity The sensitivity of the level detector can be tuned by adjusting the speed of the syringe and Z drive during a Search move A value of 125 works with a 2500ul syringe but can be adjusted here if necessary Figure 59 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 Applications Selecting the Applications button shown in ill display the window shown below This window allows you to modify the parameters for each of the existing applications and also to create new applications The Navigate buttons allow you to select any of the Applications already defined in a list The Add After button allows you to create a new Application immediately after the current position in the list The Move Down and Move Up button allows you to move an application so that it appears in a different position in the list This is useful if you want the most commonly used application to appear at the top of the list displayed during processing see 48 Figure 60 8 4 1 Areas Selecting the Areas button displays a screen allowing you to specify which Area files contain the coordinates of the racks you ar
66. ollection Wet mode will give the best reproducibility as the amount of air in the system is minimised Dry mode is a slightly simpler configuration and is more than adequate for pipetting operations where the reproducibility is not critical such as in sample archiving Note that in Dry mode although the hydraulic path between the syringe and the probe is left dry the syringe itself needs to be lubricated with a small amount of water or Silicone Oil to ensure the pistons do not wear out 3 6 3 Connecting the System Fluid Bottle Wet Mode Only The System Fluid Bottle See 1s connected to the instrument by a single length of tubing terminating in a threaded syringe connector This connector should be screwed into the right hand port of the syringe valve The other end of the tubing should be inserted through the small hole in the blue cap There is a short length of rubber sleeving which should be slipped over the tubing and positioned in contact with the underside of the blue cap The position of the sleeve should be adjusted so that the tubing rests on the bottom of the System Fluid Bottle when the cap is in place System Fluid Syringe Valve o 9963 234 onnector System Fluid Tubing Probe Syringe Tubing Figure 11 Note It is not necessary to connect the System Fluid bottle if you are only intending to use Dry mode 3 6 4 Washbowl Tubing connection The Washbowl is connected to a length of silico
67. on The fuse rating is as follows 115 to 240 Volts 2 Amp fuse Note that both neutral and live are fused 3 8 1 Power cord Where it is not known what plug type is required the power cord will be supplied without a plug If this is necessary make the following connections Live Line Brown Neutral Blue Earth Ground Green Yellow It is essential that an earth be connected to any liquid handling equipment especially if it has a filter fitted If a fused plug is used 1t requires a 3 amp fuse Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Software Installation 4 1 Computer Minimum Specification HARDWARE DETAILS COMMENTS Processor Pentium II Processor Speed 500 MHz Memory RAM 64 Mb Hard Disk 6 4 GB Optical Drive CD RW Required for making permanent backups Serial Port 1x RS232 For communication with the instrument Sound card integrated or separate Sound card speakers Used for audible warnings card Printer Most should work but For printing of Worklists should be tested first OPERATING SYSTEM Microsoft Windows Vista not yet supported XP Professional Service Pack 2 It is also strongly recommended that you incorporate a writeable e g CD RW drive for backup purposes as some of the files are larger than 1 4 MB A memory stick may also be used but the files must be archived to a disk so that a permanent record is kept It is also recommended that you purchase a printer with a USB connection Please no
68. ons is the best means of cleaning the painted and stainless steel parts of the instrument All surfaces should be routinely cleaned with decontaminating agents 9 3 2 Sterilisation Sterilisation of the liquid handling components should be performed regularly particularly if bio hazardous materials are being processed Please refer to Appendix A for recommended solutions that can be safely passed throughout the liquid path After sufficient contact time the probe s should be primed with at least 20ml of de ionised distilled water Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Before performing any maintenance functions on the instrument ensure that it is completely free of any process related hazards Certification to this effect will be required by anyone handling any parts of the instrument after they have been used This applies particularly when servicing is due or when parts are being returned for any reason 9 4 Replacing parts 9 4 1 Syringe To replace a syringe you should first ensure that the syringe piston is positioned at the bottom of its stroke This can be done using the following method If using Wet mode if using Dry mode proceed straight to step 1 below Remove the System fluid tubing from the diluent bottle Select the prime option from the RunTime main menu Press the Function key 10 when the syringe piston is travelling down The piston should stop at the bottom of the stroke Next switch off
69. ould damage the instrument and will at the very least affect its alignment Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 4 Identifying the main components of the instrument Having carefully unpacked the instrument you will find a number of components in the accessory box The following photographs help you identify each of the se components and where they should be located on the instrument Installation is mostly straightforward but details of how to install the critical components such as the probe are provided below C O h Z Rack 9972 260 1 fluig Security Bar Waste Tip Bag Support Deck i Guide Rail Figure 2 Sample and Deep Well Rack 7030 093 Syringe Drive System Fluid Bottle 9972 205 Figure 3 Washbowl Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 3 5 Levelling the instrument 3 5 1 Adjusting the feet The Xsp 220 is built around a rigid chassis which rests on 4 adjustable feet It is designed to be installed on a flat bench but adjustments can be made if necessary to accommodate a small degree of unevenness Looking at the underside of the instrument you should see all 4 feet touching the bench and there should be no rocking If there is you should adjust the feet by turning one or more feet as required Tilting the machine back can access these Get someone to help you do this The feet on the Xsp 220 have a click stop to give
70. ource rack to an Archive plate Each one is described 6 5 1 Archiving If you select the Archiving application you will first be prompted to enter the number of samples you want to process Mumber of tubes Figure 49 Next you will be prompted to enter the name of the data file that will store all the data associated with this run A default name will be displayed comprising the letters RUN a the current time and date stamp year month day hour minutes seconds The default name is designed to be automatically unique and easily ordered when searching in Explorer with respect to the time the file was created Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Name of data file lo create RUN20080000140303 Figure 50 You will then be prompted to enter the barcode of the sample tubes It is best to do this as you load each sample into the XSP 220 rack The display also shows a representation of the rack with a pointer indicating the position of the next tube to be read Y ou should enter the barcode using the Wedge reader or if damaged you may enter it manually To go back to the previous position simply enter a blank ID AAA EEE T e a tas Oa A a ps al sas To Te erp ees oo ees Clee eee so A b ME BEF Er ee EE Figure 51 Finally you will be asked to asked to ACCEPT the entries and you will then be prompted to load both the target tubes and Source tubes Figure 52 Place circe hiba im
71. r Manual 4 6 6 Passwords When you first install the software onto a PC you will need to login to the password system in order to disable the default requirement for passwords Select Login and enter supervisor and password in lower case as the User Name and Passwords respectively Enter ques uses nemn n panel beef User Marne re Password Lore w Figure 28 Fabs ineng Frari Enar Peed EA Figure 29 You should then get a message to inform you that you are logged in You may now uncheck the Enable Password Protection option Figure 30 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 6 7 Logging Make sure that both the Link Log and Error Logs are both checked These instruct the instrument to automatically record certain messages in a file eg BEEDRIVE LOG which can be used by a product specialist to help find the fault See Figure 31 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 7 Updating Software If you need to re install or update the software you will first need to perform an uninstall Select Start gt Control Panel gt Add or Remove Programs Scroll down and select Visprog2 and then select Remove After completion repeat and select XSP 220 Applications and select Remove You may now re install both sets of software 4 8 Operating System Settings 4 8 1 Windows XP Professional If you opt to use certain features in the so
72. s available in your laboratory at all times Demo Consumables Disposable tips 1200ul volume in wafer pack 768 tips TIP 1005 Dilution tubes in strips of 8 pack of 1000 2685 013 Syringe 1 0ml 7350 107 Syringe 2 5ml 7350 108 Diluent Tubing and Sleeve for System Fluid Bottle 9972 208 Beeline 220s Installation Kit KIT1022 Autoclavable bags for waste tips Pack of 100 MME9361 DT Probe 9967 201 Tip adaptor compatible with TIP1005 3432DA Autoclavable waste tip bags MME9361 Racks Disposable tip rack for 1200ul wafer tips non sterile 7030 092 96 posn x13mm Diameter Sample 1 x SBS Format 7030 093 location Rack locator Lockable 9967 410 Rack Locator Standard with SS grub screw 9972 218 Z Drive Z Rack 4 0mm ID with end stop insert 9972 260 Probe Z guide 9972 502 Syringe Drive Solenoid Valve Assy 3 Port c w cable amp washer LIQX 9963 234 Solenoid Valve Syringe Adaptor PTFE washers 9972 501 Miscellaneous Fuses 2 Amp T type Pack of 10 2070 002 Unblocking kit Included in KIT1022 9963 500 Disposable Syringe 20ml Unblocking kit FLU9746 Coupler Unblocking kit HOC9709 Silicone Grease 100gram tube 2800 025 Installation kit KIT1022 Wedge barcode reader Cipher BCR1005 Wire for unblocking probes 2mtr 7650 006 Probe Support rod 6320 040 System Fluid Bottle assembly including tubing 9972 205 DT Probe Compression Spring MME 9357 DT Knurled locking collar 3427 Choice depends on which sizes have been instal
73. t tube Actual Transfer Volume The volume of liquid actually transferred to the target Wastage volume Status A text string indicating the status of the operation specified on the current row of the worklist typically PENDING OK or ERROR Diluent Position The position within the rack specified by the application from where to aspirate diluent Reagent Position The position within the rack specified by the application from where to aspirate reagent Source Tube alias ID Stream 8 5 4 Well Number Format Click Well Number Format on the Worklist Configuration Menu The Well Number Format window will appear wei Hunt Farma Figure 84 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 5 4 1 Source Area Well Number Format The XSP 220 can handle 3 different formats of microtube rack position or microplate well number Numeric where the positions are numbered from 1 upwards Alphanumeric where the positions are numbered from Al to H12 Numeric positions match the way the instrument Areas are defined For example a 96 position microplate may have its wells numbered in 12x8 or 8x12 orientations the direction of the numbering may be done in various ways as well Alphanumeric numbering is assumed to be done in the same way as the conventional numbering of a microplate with position 1 being A1 12 being A12 and 96 being H12
74. tails Run End of Day Protocol Clean Probe Nozzles with Methanol Weekly Details Clean Z rack if necessary Run End of Day Protocol Run Decontaminate with Medis Dist H20 Document 9967 901 Issue 1
75. tain the same sequence in both Source and Target Another reason is that you might always want the option of trying to manually pipette some sample into the archive plate Set to 0 if you do not want an empty position Set to to leave a space in the target rack normal Los space H con process Y v Ember ium me paco in im angat ac neu a peen in im targat rack Figure 71 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 3 11 Source and Target Areas overlap Leave this set to No Ture And tren as lag T Estar You the sown suj torget ones whore ter A a Figure 72 8 4 3 12 Barcoded source tube search flag This option apples if an automatic barcode reader is installed on the instrument It allows you to instruct the instrument whether or not to search for a tube within a rack if it is not in its expected location You should set this 0 for the XSP 220 Ber coded somen tube search Mag Ester on seerching 1 search miihiim specified rack 2 snamh all recia Figure 73 8 4 3 13 Worklist flag This controls the use of a worklist Enter 0 if no worklist is to be created Enter to create a worklist during execution Enter 2 to use an existing worklist If you use the same worklist more than once it will simply overwrite any existing data Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Ester nn warkdiel 1 creaba worklint Figure 74 8 4 3 14 Method of
76. te that HTZ only supports the English Language Version of Windows XP If you are unable to obtain this version or need to use another language version please contact HTZ first for advice 4 2 Software Overview If the PC was supplied with the instrument the software will have been installed and configured at the factory However if the PC was sourced locally it will be necessary to perform the software installation procedure described below There are 3 main components to the Xsp 220 Software supplied on 3 CDs 1 VisProg System the main instrument control software 2 Applications Software a specific piece of software for an application on this configuration of instrument 3 Instrument Configuration XYZ calibration data for an individual instrument Each of these disks needs to be installed 4 3 Visprog Installation New Installation 1 Insert the disk labelled VISPROG2 Installation and Set up CD into the CD drive of the computer The program should now automatically load If it does not locate and run the SETUP EXE program located in the root directory of the CD by selecting the Windows Start button Start Run and Browse 3 Follow the screen prompts pressing the Next Button when prompted Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 Accept the default Program Folders Directory See Figure 17 pnd click Next Samir Precessor 42 Hu Figure 17 5 Click Next on the
77. the Lock Nut clockwise until the spring is compressed by approximately 50 as shown in Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Fluidic Components of Xsp 220 Water filled Pressure Sensor on back of instrument Pressure Sensor Tube Short Syringe Tip Adaptor _ ______ Pipetting Needle long Pipetting path Disposable Tip System Fluid Pressure Sensor Path DI water Sample tube ______ The Fluidic and Sensor path for the XSP 220 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual Pipetting nozzle Sensor nozzle Figure 7 Apply a very small amount of silicone grease onto the thread DO NOT ALLOW ANY GREASE TO TOUCH THE NEEDLES AS THIS MAY CAUSE THE LEVEL SENSOR TO FAIL Screw the tip adaptor onto the thread After approximately 3 turns it will suddenly become much more difficult as the thread on the adaptor is tapered From this point you should continue to turn the adaptor by approximately 34 to 1 turn which will ensure a good gastight seal Use some fine emery cloth P600 or rubber gloves to grip the adaptor if necessary Now turn the locking nut anti clockwise so that it is tight on top of the tip adaptor The spring should now be fully uncompressed and the gap between the top of the adaptor and the bottom of the Z rack approximately 12 mm As shown in Figure 8 Document 9967 901 Issue
78. the instrument 1 Undo the knurled locking ring underneath the syringe drive plate by turning it clockwise viewed from above and then move the piston up into the syringe 2 Holding the syringe barrel at the top with one hand and the plastic adapter the syringe is screwed into with the other unscrew clockwise viewed from above If the plastic adapter comes away from the valve with the syringe then unscrew it from the old syringe Ensuring that the white adapter teflon washer is still in place inside the adapter screw it back into the valve block Also be aware of a small valve teflon washer which is fitted inside the valve This sometimes falls out and will cause leakage around the top of the adapter see fig below Taking the new syringe ensure the piston is pushed into syringe barrel Screw the syringe into the plastic adapter in the valve block Pull the piston rod down and pass the threaded end through the syringe drive plate Screw the knurled locking ring back onto the piston rod A UD Knurled Syringe Locking Ring Piston Syringe Syringe Tip PO Barrel Valve teflon washer Adaptor 9972 501 Syringe thread wae Adaptor teflon Packing Piece washer Syringe Unit 9 4 2 Probe First remove the existing probe This is the reverse of the installation procedure as described in Section Next install the probe as per the installation procedure You should then check that the co ordinates of the new probe correspond to
79. thin a row is the same The distance between each row 1f more than 1 is the same The required sequence of processing i e direction for each row is the same Rack An item designed to hold one or more containers test plates slides etc If an Area contains multiple locations these are numbered as Rack 1 Rack 2 Rack 3 etc The line of positions in a Location that defines the order in which the positions are processed Processing normally proceeds in the direction of whatever is defined as a row When it reaches the end of one row it normally proceeds to the beginning of the next row See Page amp Snake sequence below Some Locations may only have one Row in which case the end of the first row and the end of the last row are defined as the same co ordinate When defining a Location you will need to specify the number of positions in a row and the number of rows per Location When defining an Area with Multiple Locations these numbers will need to be defined for each Location Virtual Joystick The software tool that allows you to move the probe to anywhere on the bed using the mouse Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 6 Running the Xsp 220 6 1 Preparing the Xsp 220 for Use Must be pushed down so that it clicks into position Switch on the power for the Xsp 220 switch is at rear right hand side of the instrument The power switch for the Xsp 220 is located at the rear
80. tional X movement will drive the arm into the right hand cover Lleida Tash bee Sus Cove ican ip Ems a FAA ria ek EE Te Figure 23 Security Cover Configuration 4 6 3 7 Plate Incubator Select this option only if your instrument is fitted with the microplate incubator option If you check the Autorun option the instrument will switch on the incubator as soon as the Runtime software is loaded You will be asked to enter the temperature This would normally be set at 37 C Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual hat I tiza Im Figure 24 Incubator Configuration 4 6 4 System 4 6 4 1 Task Bar The first option on the System Menu is the Task Bar This controls which of the Background programs are displayed on the Task Bar at the bottom of the screen when the Xsp 220 is running These background programs are so called because under normal operation you don t need to use them This configuration should therefore normally be set as shown below Tege Gemend ficial ls Lega We The Taria ps re a Topi Fer Pas repisa Dira M Tea eena la e ed Lts e ng Figure 25 Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 4 6 4 2 Sensed Bottle Rack The Sensed Bottle Rack option relates to a set of special bottles incorporating sensors These are currently not available for the Xsp 220 so this option should be unchecked Tester Geared Goes Cos Legs
81. uid on the probe Redefine the Search Height and reaching liquid surface before reaching the Search Height make sure it is above the maximum during a search move possible liquid level that will be In a Search move the probe moves down encountered for that Area Probe returns to Washbow l as it from the Clearance Height to the Search has found liquid before reaching Height and then commences looking for the Search Height liquid If the level detector is triggered Attempts to retry fails a second before reaching the Search Height an error is time and returns to Washbowl generated The Probe has therefore found Probe does a dummy dispense liquid before it should have and moves onto to aspirate next sample The Search Height parameter in the Area Error is generated Definition may be set to a lower height than Pipetting code on screen Blue the maximum possible liquid level for that Pipetting code in Activity Log container and this particular tube has been Wet filled above the normal fill level 14 Probe sensitivity has been set too high and Adjust probe sensitivity the probe is sensing liquid before the Search move starts or before it reaches the Search Height 15 Probe electrical connections or wiring have Replace probe been damaged and the probe is producing an iptermittent short circuit 5 Failed to dispense correct 16 Henao Diameter in rea set too large so Redefine Area and make sure probe amount of sample or reagent probe not trac
82. y the accuracy and precision of liquid handling and electrical safety 9 2 Daily Routine These procedures do not need to be followed if the system liquid is de ionised or distilled water If it is then ignore the daily tasks concerning the liquid path Ensure the Tip Adaptor is wiped clean You may use isopropyl alcohol IPA Maintenance 9 2 1 Probe and fluid path tubing If evidence of a leak is apparent and the valve connectors are acceptably finger tight it is possible that the flanges on the probe tube or system liquid tube might be damaged When these have been emptied of liquid the connectors can be removed from the valve and the flanges examined If anything but a flat round concentric flange is observed this may be the cause of the leak If this is the reason the tubing or probe needs to be replaced 9 2 2 Valve assembly A very slight leak will not affect performance unduly but if drips form on the probe when primed in the home position then the valve will have to be replaced as soon as possible Before you reject the valve assembly ensure that the tube connectors are all finger tight 9 2 3 Syringe drive unit Inspect the liquid path for any signs of leakage Check the junction between the valve barrel under the piston seal and at the base of the barrel A serious leak in any of these positions will probably require replacement of the syringe unit If using Mode 2 no liquid in probe you should ensure that there is alwa
83. y means of a layout file A layout file is a simple text file that specifies which positions will be processed and is useful if a certain number of positions are always omitted eg the first row of a rack or perhaps the first postion in every row If set to 2 the sequence of positions will be read from a specific field within the Worklist This allows the sequence to be different for every batch if required Layout File Option not yet implemented Somen pasilino Marj Ember anquenttal uma jopon Hio 2 rand irom verkini Figure 69 8 4 3 8 Target Position Flag This is analogous to the Source Position flag and controls the sequence of positions in the Target rack For archiving this would usually be set to 0 i e samples loaded into sequential positions Ember anquenttal wee japoni file 2 rand irom verkini Document 9967 901 Issue 1 HTZ Xsp 220 Operator Manual 8 4 3 9 Full path of layout file If a layout file is specified for either the Source or Target positions you should enter the name of the file here e g C XSP layouts layout txt Cabar dhe bell path af the lope Wile lee bla met cand nr mirad m ran time Figure 70 8 4 3 10 Leave space if cannot process This controls what happens to the loading of the Target rack in the event it encounters a tube which it cannot pipette e g if there was no liquid detected in the tube The value you chose will depend on whether you always want to main
84. ys a small amount of liquid in the syringe barrel to lubricate the piston You may use either water or a few drops of silicone oil Introduce this into the syringe by first removing it see from the instrument withdraw the piston and with the barrel upside down add a few drops of liquid into the barrel Replace the piston and remove the air from the syringe before installing it back into the syringe drive 9 2 4 Lubrication The Z Rack should be inspected every week to see there is any excessive accumulation of debris and residual lubricant If itis wipe the smooth surfaces with a cloth soaked in a mild detergent then using a small brush clean the gaps between all the gear teeth to remove as much dirt and debris as possible After cleaning the Z Rack re lubricate it by moistening the assembly with a thin coating four drops light machine oil e g WD40 or similar Avoid any excess as this will be taken up by the drive pinion and thrown outwards fouling the sensors If this happens errors may occur with the Z motion 9 2 5 Electrical safety Periodically inspect the mains lead for damage to the insulation or connections at both ends If any damage is found insulate the damaged area and order a replacement lead as soon as possible 9 3 Cleaning the Instrument 9 3 1 Cleansing agents Do not use abrasive cleaning agents as these will damage painted surfaces A cloth soaked in a mild detergent solution refer to Appendix A for recommended soluti

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