Dynamic Mixer Manual
Contents
1. 2 j A al 6 3 4 Figure 2 Plumbing for a typical binary gradient HPLC Where Pressure module Pump A Pump B Mixer Prime Purge valve Column Injection valve Detector o E E set Publication no 0391467400 Issue 5 February 2009 Page 9 of 28 Varian Dual Chamber Dynamic Mixer he Caution Note Warning Chemical Hazard Death serious injury or damage to skin can result from contact with chemicals used in HPLC especially when under high pressure Always wear appropriate protective equipment and clothing To prevent rupture or component damage the maximum working pressure of the HPLC system should not exceed the rated value of any component within the HPLC system To connect the mixer to your HPLC system 1 Connect the high pressure outlet lines from the pumps to the mixer inlet ports 2 Connect the mixer outlet to the prime purge valve For normal use flow from the pumps is directed through the injection valve and the column 3 Connect the outlet from the prime purge valve to the injection valve and then to the column 4 Connect the column outlet to SAMPLE IN on the detector and SAMPLE OUT to waste or a fraction collector Connect any external pressure module into the HPLC system as shown in Figure 2 2 2 1 Ternary Gradient HPLC Connections Page 10 of 28 Ternary gradient HPLC connections are identical to that of the binary gradient system except that the high pressure outlet2. body lightly to loosen them Unscrew the seal retainer and pull the seal off the piston Preparative Mixers Turn the mixer over to remove the stir bar cage The stir bar cage contains two stir bars and is capped at both ends Disassemble the stir bar cage by pulling off each stir bar cap Remove both stir bars Preparative scale mixers do not use a seal retainer Simply pull the seal off the piston on these models Warning Chemical Hazard Death serious injury or damage to skin can result from contact with chemicals used in HPLC especially when under high pressure Be careful not to spill solvent from the mixer body bore during this procedure Always wear appropriate protective equipment and clothing Publication no 0391467400 Issue 5 February 2009 Page 19 of 28 Varian Dual Chamber Dynamic Mixer 3 3 3 4 3 4 1 3 4 2 Page 20 of 28 Caution Do not use sharp instruments to remove the stir bars the spacer or the stir bar cage The mixer components or the mixer body bore may be damaged 8 The mixer components are now fully disassembled and can be cleaned or replaced as necessary Replacing the frit and seal are described in Sections 3 5 and 3 6 Cleaning the Mixer Components Unless the mixer components are badly contaminated they are best cleaned by wiping with a lint free wipe and using alcohol to remove any small particulate matter If the mixer is badly contaminated cleaning all fluid path
3. components by sonication is recommended Mixer Reassembly Perform the steps in either Section 3 4 1 or Section 3 4 2 depending on your mixer type Then proceed to Section 3 4 3 to complete the procedure Analytical and Narrowbore Mixers 1 Take one stir bar and hold it above the mixer bore so the cross vanes are up and down Drop the stir bar into place Drop the spacer into the bore then the upper stir bar 2 If you removed the seal take a replacement seal and place it on the piston so the spring inside the seal is visible If the seal is put in upside down the seal will leak Push the seal over the screw threads onto the shoulder Thread the seal retainer onto the piston and finger tighten Do not use tools Preparative Mixers 1 Reassemble the stir bar cage place one stir bar into one end of the stir bar cage and press fit the stir bar cap Both ends of the stir bar cage are symmetrical and the cage fits into the mixer bore in either direction 2 Slide seal so that the spring cavity is facing downward Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 3 4 3 All Mixers 1 Press the piston with the seal now attached with seal retainer on analytical scale models into the mixer body bore as far as possible 2 Finger tighten the mixer cap Do not over tighten 3 Reconnect the outlet line to the mixer Tighten the outlet fitting finger tight then tighten a further 1 3 turn if u
4. lines from pumps B and C are connected through a Tee to the mixer inlet ports Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer A 7 2 6 10 of 8 3 E 4 11 Figure 3 Plumbing for a typical ternary gradient HPLC Where Pressure module Pump A Pump B Pump C Tee Mixer Prime Purge valve Column Injection valve 0 Detector 1 Fraction collector tA a ODO AON OaPrWON Warning Chemical Hazard Death serious injury or damage to skin can result from contact with chemicals used in HPLC especially when under high pressure Always wear appropriate protective equipment and clothing Caution To prevent rupture or component damage the maximum working pressure of the HPLC system should not exceed the rated value of any component within the HPLC system Publication no 0391467400 Issue 5 February 2009 Page 11 of 28 Varian Dual Chamber Dynamic Mixer Note If your system has an external pressure monitor plumb it between one pump and either the mixer or the tee as shown in Figure 3 2 3 Plumbing Fittings 2 3 1 0 6 mL and 1 2 mL Stainless Steel Mixers For the extra long fitting on the outlet and the two standard fittings for the inlet slide the fitting and ferrule over the 1 16 in OD tubing and push the end of the tubing into the port as far as possible Holding the tubing in place finger tighten the fitting then tighten 14 turn more wi
5. mixer in good condition Always use HPLC grade solvents Impurities present in other grades may contaminate system components Always use a solvent inlet filter to reduce particulate contamination and to prevent clogging of the mixer frit Never mix or make a direct change between immiscible solvents Change between them via an intermediate solvent or a series miscible with both After using concentrated salt solutions flush the mixer thoroughly with water then with 50 50 MeOH water Leave the solution in the mixer overnight Before using the salt solution again flush with water thoroughly If flushing is not practical set a low flow rate 0 01 mL min to avoid salt precipitation Avoid knocking or jarring the mixer Although rugged it should always be treated with the degree of care appropriate for laboratory instrumentation 3 2 Mixer Disassembly Figure 8 shows an exploded view of the internal components of an analytical or narrowbore mixer body Figure 9 shows the view of the preparative mixer Publication no 0391467400 Issue 5 February 2009 Page 17 of 28 Varian Dual Chamber Dynamic Mixer 6 8 12 11 Figure 8 Exploded view of an analytical or Figure 9 Exploded view of a preparative mixer narrowbore mixer body body Where Where 1 Outlet port 1 Outlet port 2 Mixer cap 2 Mixer cap 3 Piston 3 Piston 4 Seal spring down 4 Seal spring down 5 Seal retainer includes fr
6. 2 5 Mounting 14 3 Maintenance and Troubleshooting 17 3 1 Operating Precautions 17 3 2 Mixer Disassembly 17 3 3 Cleaning the Mixer Components 20 3 4 Mixer Reassembly 20 3 4 1 Analytical and Narrowbore Mixers 20 3 4 2 Preparative Mixers 20 Publication no 0391467400 Issue 5 February 2009 v Varian Dual Chamber Dynamic Mixer 3 4 3 All Mixers 21 3 5 Checking and Replacing the Outlet Filter Frit 22 3 6 Seal Replacement 23 3 7 Troubleshooting 24 4 Spare Parts 25 5 Specifications 27 vi Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer General Safety Precautions Follow these safety practices to ensure safe equipment operation Perform periodic leak checks on all supply lines and pneumatic plumbing Store organic solvents in fireproof vented and clearly labeled cabinets so they are easily identified as toxic and or flammable materials Do not accumulate waste solvents Dispose of such materials through a regulated disposal program and not through municipal sewage lines This instrument is designed for chromatographic analysis of appropriately prepared samples It must be operated using appropriate gases and or solvents and within specified maximum ranges for pressure flows and temperatures as described in this manual If the equipment is used ina manner not specified by the manufacturer the protection provided by the equipment may be impaired It is the responsibility of the Custom
7. 3503201 Transformer adapter AC AC 230 to 9 VAC R007000507 Mixing seal retainer with Titanium frit 10 um R007000054 Inlet solvent filter 2 um 10 mm part number R000083915 R007100151 Seal retainer assembly 1 2 mL mixer R002540006 Seal stainless steel 10 mm R002540009 Mixing seal Titanium 10 mm R002540014 Pressure seal 0 715 in ID UHMW perf 1500141000 Nut GC 1 16 in SST 2869453300 Ferrule 1 16 in CPI SST R007010062 Bushing extra long ROOOFTF130 Male nut 10 32 PEEK ROOOFT F300 High pressure nut ferrule PEEK 10 32 ROOOOLT200 High pressure ferrule PEEK 4 28 x 1 8 in OD tubing ROOOOLT210 Nut PEEK 14 28 x 1 8 in OD tubing R002500002 Magnetic mixing stir bar 3 8 in x 5 16 in 0393502801 Mixer mounting kit Publication no 0391467400 Issue 5 February 2009 Page 25 of 28 Varian Dual Chamber Dynamic Mixer This page is intentionally left blank Page 26 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 5 Specifications Part Number Product Materials in Fluid Contact Scale 0393502100 Narrowbore Mixer 316 Stainless Steel Teflon Hastelloy Narrowbore 0 6 mL SS Fluoroloy 12 0393509700 Narrowbore Mixer Titanium Teflon Fluorowhite Narrowbore 0 6mL TI 0393501801 Dual Chamber Mixer 316 Stainless Steel Teflon Hastelloy Analytical 1 2mL SS Fluoroloy 12 0393502001 Dual Chamber Mixer Titanium Teflon PEEK UHMWP
8. E Analytical 1 2 mL PK Fluorowhite 0393501901 Dual Chamber Mixer Titanium Teflon Fluorowhite Analytical 1 2 mL TI 0393502301 Dual Chamber Mixer PEEK Teflon Perfluoro Fluorowhite Preparative 10 mL PK 0393502201 Dual Chamber Mixer Titanium Teflon Perfluoro Fluorowhite Preparative 10 mL TI Required Tube OD Mixing Chamber Mixing Chamber Volume Total Fittings Pressure Rating Max in Each Chamber Volume 10 32 1 16 in 41 4 MPa 6000 psi 0 3 mL 0 6 mL 10 32 1 16 in 27 6 MPa 4000 psi 0 3 mL 0 6 mL 10 32 1 16 in 41 4 MPa 6000 psi 0 6 mL 1 2mL 10 32 1 16 in 27 6 MPa 4000 psi 0 6 mL 1 2 mL 10 32 1 16 in 41 4 MPa 6000 psi 0 6 mL 1 2mL 28 1 8 in 13 8 MPa 2000 psi 5 mL 10 mL 28 1 8 in 41 4 MPa 6000 psi 5 mL 10 mL Publication no 0391467400 Issue 5 February 2009 Page 27 of 28 Varian Dual Chamber Dynamic Mixer Item Specification Motor speed Operating voltages 300 rpm at 60 Hz line frequency 250 rpm at 50 Hz line frequency Line voltage 120 230 240 VAC Unit voltage 9 VAC Power Operating frequency 8 VA 50 60 Hz Power ratings transformer dependent USA Wall transformer 9 VAC 500 mA Europe Wall transformer 9 VAC 500 mA UK Wall transformer 9 VAC 500 mA Indoor use 4 60 C Operating temperature range Relative humidity 10 to 90 Altitude Up to 2000 m Installation category 11 Pollution degree 2 Weight Appr
9. Varian Dual Chamber Dynamic Mixer Instruction Manual Installation Category II Pollution Degree 2 Safety Class 1 EN 61010 1 ap 2 lt Publication no 0391467400 Issue 5 VARIAN February 2009 Varian Dual Chamber Dynamic Mixer Varian Inc Serving Industries Worldwide Biosciences Pharmaceuticals Clinical Research and Forensics Food and Agriculture Chemical Analysis Environmental Fuels and Energy Material Sciences Sales and Service Contact Details North America 800 926 3000 925 939 2400 Europe The Netherlands 31 118 67 1000 Asia Pacific Australia 613 9560 7133 Latin America Brazil 55 11 3238 0400 Varian Inc Web Site www varianinc com Varian Inc is the owner of copyright on this document and any associated software Under law the written permission of Varian Inc must be obtained before the documentation or the software is copied reproduced translated or converted to electronic or other machine readable form in whole or in part Updated February 2009 Varian and the Varian logo are trademarks or registered trademarks of Varian Inc in the U S and other countries 1998 2009 Varian Inc Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer CE Declaration of Conformity We hereby declare that the equipment listed below complies with the requirements of The Low Voltage Directive 73 23 EEC 93 68 EEC The EMC Directive 89 336 EEC 92 31 EEC an
10. body Where ONOaROD 10 32 fitting extra long Piston Mixer cap Seal Drive magnet Seal retainer Frit Stir bar upper 9 Spacer 10 Shaft 11 Stir bar lower 12 10 32 fitting standard 2 each Page 6 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer Solvents from the pumps enter the mixer via the two inlet ports at the base of the mixer body They flow into the lower mixing chamber where they are mixed by the rotation of the lower stir bar The pressure from the pumps forces the mixed mobile phase upward through the spacer into the upper mixing chamber where additional mixing is performed by the opposite rotation of the upper stir bar The fully mixed mobile phase is then forced through a 2 um frit in analytical and Narrowbore mixers through the piston and out to the rest of the HPLC system Since the spacer the stir bar cage on preparative mixers assures isolation between the two mixing chambers the Varian Dynamic mixer acts as a two stage filter for solvent composition noise It is more effective in averaging and reducing solvent composition noise than a single chamber mixer of equivalent volume The passive seal used in Varian Dynamic mixers is a hollow molded plastic ring with a circular groove containing an energizing spring The side of the seal containing the spring faces into the mixer chambers When the mixer is unpressurized the small sp
11. d 93 68 EEC Applicable Standards LVD BS EN 61010 1 1993 EMC BS EN 61326 2003 Equipment Model Number Varian Dual Chamber Dynamic Mixers Authorized Representative in the EU Name G A Wassink Company Name Varian BV Address Herculesweg 8 Aj 4338 PL Middelburg The Netherlands Position Quality Manager Telephone 31 0 118 671 000 Date 1 February 2009 Facsimile 31 0 118 633 118 Manufacturer Signed Name Philip Binns Company Name Varian Australia Pty Ltd Address 679 Springvale Road tit Mulgrave VIC 3170 f P i fie Signed Position Director of Operations Telephone 61 0 3 9560 7133 Date 1 February 2009 Facsimile 61 0 3 9560 7950 Publication number 8510251600 February 2009 Publication no 0391467400 Issue 5 February 2009 iii Varian Dual Chamber Dynamic Mixer This page is intentionally left blank iv Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer Contents General Safety Precautions 1 CE Compliance 1 Electrical Hazards 1 Note Caution and Warning Messages 2 Service Availability 3 1 Introduction 5 1 1 General 5 2 Installation 9 2 1 Unpacking 9 2 2 Plumbing 9 2 2 1 Binary Gradient HPLC Connections 9 2 2 1 Ternary Gradient HPLC Connections 10 2 3 Plumbing Fittings 12 2 3 1 0 6 mL and 1 2 mL Stainless Steel Mixers 12 2 3 2 1 2 mL PEEK 0 6 mL Titanium and 1 2 mL Titanium Mixers 12 2 3 3 10 mL PEEK and Titanium mixers 13 2 4 Electrical Connections 13
12. er to inform Varian Customer Support Representatives if the instrument has been used for the analysis of hazardous biological radioactive or toxic samples prior to any instrument service being performed or when an instrument is being returned to the Service Center for repair CE Compliance This product complies with the requirements of the European Union which allow the CE marking to be affixed Electrical Hazards Replace faulty or frayed power cords immediately with the same type and rating Make sure that voltage sources and line voltage match the value for power supply module Publication no 0391467400 Issue 5 February 2009 Page 1 of 28 Varian Dual Chamber Dynamic Mixer Page 2 of 28 Note Caution and Warning Messages Note Caution This instruction manual is provided to help you establish operating conditions which will permit safe and efficient use of your equipment Special considerations and precautions are also described in the manual which appear in the form of Notes Cautions and Warnings as described below It is important that you operate your equipment in accordance with this instruction manual and any additional information which may be provided by Varian Address any questions regarding the safe and proper use of your equipment to your local Varian office A note provides information to aid you in obtaining optimal performance from your instrument A Caution message is used when failure to observe i
13. f an HPLC pump 1 Remove one of the screws on the rear side of the pump 2 Screw the mounting post into this hole and tighten with a wrench 3 Thread the mixer onto the other end of the mounting post and rotate the instrument clockwise four times to engage the threads 4 Lock the mixer in position by tightening the mounting nut with a wrench To mount the mixer to the mast Instead of using the mounting post fasten the mast clamp to the mixer with the 10 32 x in screw provided The mast clamp can be fitted to the mast and tightened into position with the T screw Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer Figure 7 Hardware for mounting the mixer Where 1 Mounting post 2 Mounting nut 3 Screw 4 Mast clamp Publication no 0391467400 Issue 5 February 2009 Page 15 of 28 Varian Dual Chamber Dynamic Mixer This page is intentionally left blank Page 16 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 3 Maintenance and Troubleshooting The uncomplicated design and rugged construction of the Varian Dynamic mixers help to keep maintenance to a minimum When maintenance is required the mixer body can be easily disassembled without tools Maintenance is limited to the hydraulic components and fluid pathways through the mixer body 3 1 Operating Precautions The following operating precautions will help to keep your
14. gnet turns it causes two magnetic stir bars inside the chambers to rotate by radial drive rather than by axial drive as in other dynamic mixers The close proximity of both stir bars to the rotating drive magnet and the fact that the stir bars rotate in opposite directions ensures continuous and thorough mixing Power is provided by the 9 VAC external transformer Exploded views of the mixers can be seen in Figure 8 and Figure 9 in the Maintenance and Troubleshooting section A unique piston type closure on the outlet of the mixer allows easy disassembly without tools for cleaning and maintenance On analytical and narrowbore mixers the piston incorporates a 2 um solvent filter to protect system components from contamination The mixer is designed to be plumbed into an HPLC system between the pumps and the injection valve A mounting post and a mast clamp come with the each mixer to allow it to mount to the side of various HPLC pumps or to mount to the mast of an HPLC system Titanium and PEEK Varian Dynamic Mixers are available for biochemical applications where 316 stainless steel may be inappropriate because of corrosion or release of metal ions into solution Titanium or PEEK plumbing components can be used together with PEEK tubing to provide a totally iron free fluid path Publication no 0391467400 Issue 5 February 2009 Page 5 of 28 Varian Dual Chamber Dynamic Mixer Figure 1 Section view of the Varian Dynamic analytical mixer
15. gure 6 Compression fittings for 10 mL PEEK and 10 mL titanium preparative mixers Where 1 Fitting 2 Ferrule collar 3 Ferrule 2 4 Electrical Connections All Varian Dynamic mixers are designed to run from a 9 VAC transformer Plug the transformer into a wall outlet and insert the power jack into the receptacle in the rear of the mixer An LED will light to indicate the mixer motor is turning and that power is flowing to the instrument Publication no 0391467400 Issue 5 February 2009 Page 13 of 28 Varian Dual Chamber Dynamic Mixer 2 5 Page 14 of 28 Note Lad Although the mixer motor is extremely robust disconnecting the mixer from its power supply when the system is not in use will increase the life of the motor Warning Fire Hazard To ensure power ratings are not exceeded use only the appropriate Varian supplied wall transformer The 9 VAC voltage level does not pose a shock hazard however a slight spark could occur during disconnection of the power jack or when a live power jack comes into accidental contact with metal parts on the laboratory bench If the mixer is to be disconnected while working around extremely volatile solvents the transformer should be unplugged from the wall before the power jack is disconnected from the mixer Mounting Hardware for mounting the mixer to the side of a HPLC pump or to the mast of an HPLC system is included with the mixer To mount the mixer to the side o
16. han finger tight may deform the cap or the top of the piston Never use tools to tighten the mixer cap The seal can be replaced without fully dismantling the mixer or disconnecting the outlet tubing 1 2 Follow steps 1 2 and 3 in the procedure in Section 3 2 Analytical and narrowbore mixers Loosen and remove the seal retainer and frit Remove the seal by pulling it away from the piston Place a replacement seal on the piston so the spring inside the seal is visible If the seal is put in upside down the seal will leak Push the seal over the screw threads onto the shoulder Thread the seal retainer onto the piston and finger tighten Do not use tools Preparative mixers Remove the seal by pulling it away from the piston Slide the seal over the piston so the side of the seal with the larger outside diameter is closer to the bottom of the piston Press the piston with seal and seal retainer on analytical and narrowbore models all the way into the mixer body bore Replace the mixer cap Publication no 0391467400 Issue 5 February 2009 Page 23 of 28 Varian Dual Chamber Dynamic Mixer 3 7 Troubleshooting Varian Dynamic mixers should give years of trouble free service provided they are operated and maintained as outlined in this manual However even with careful use and regular maintenance sometimes problems may still arise When troubleshooting HPLC systems start with a systematic approach and then focus on
17. it 5 Stir bar cap top 6 Stir bar 6 Stir bar 7 Spacer 7 Stir bar cage short end up 8 Stir bar 8 Stir bar 9 Mixer body 9 Stir bar cap bottom 10 Inlet port 10 Mixer body 11 Mixer housing 11 Inlet port 12 Mixer housing Page 18 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer To disassemble the mixer 1 Before you disassemble the mixer first unplug the transformer from the wall outlet and then from the mixer Unthread the mixer cap by turning it counter clockwise The outlet tubing fitting will still be connected The piston is held in the mixer body bore by the friction of the seal Pull up on the outlet fitting to extract the piston the seal and the seal retainer in analytical and Narrowbore models Disconnect the outlet fitting from the piston using two open end wrenches use a wrench across the flats of the piston to hold it in place while you loosen the outlet fitting with another wrench Finger tight fittings should be removed without tools Put the piston assembly and the mixer cap in a clean safe place Disconnect the two inlet fittings at the base of the mixer body with the wrench or without tools if finger tight fittings are used Analytical and narrowbore mixers Turn the mixer over to remove the upper stir bar the spacer and the lower stir bar These components should fall freely from the mixer body bore but you may have to tap the mixer
18. nstructions could result in damage to equipment Varian supplied and or other associated equipment The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows Chemical hazard Fire hazard The following symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure referred to by that warning label Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer Service Availability Varian provides a variety of services to support its customers after warranty expiration Repair service can be provided by attractively priced service contracts or on a time and material basis Technical support and training can be provided by qualified personnel on both a contractual or as needed basis Publication no 0391467400 Issue 5 February 2009 Page 3 of 28 Varian Dual Chamber Dynamic Mixer This page is intentionally left blank Page 4 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 1 Introduction 1 1 General Varian Dynamic Mixers are dual chamber high pressure dynamic mixers designed for binary and ternary gradient HPLC and preparative HPLC systems The unique design of the Varian Dynamic Mixer employs a motor driven magnet oriented perpendicularly to the mixing chambers As the ma
19. one component of the HPLC system at a time in a logical progression Use a process of elimination to locate any given problem Problem Leaks Possible Cause Correction Loose fittings Tighten just enough to stop the leak Damaged seal Replace the seal See Section 3 6 Damaged ferrule in Replace the ferrule Do not over tighten compression fitting See Figure 4 Incorrect fitting Reconnect with correct fittings Problem Excessive backpressure Possible Cause Correction Clogged mixer frit Analytical and narrowbore mixers only Replace the frit See Section 3 5 Stir bar spacer sticking Dismantle and clean the mixer Filter solvents Check solvent miscibility See Section 3 2 Blocked tubing Loosen the fitting after each component to find the blockage Replace the affected tubing Damaged ferrule inthe Replace the ferrule Do not over tighten compression fitting See Figure 4 Problem Unsatisfactory peaks or baseline Possible Cause Correction No power to mixer Check that the power cord is fully inserted into an outlet and into the power jack of the mixer Check that the LED indicator is on Page 24 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer Spare Parts 0393503301 Transformer adapter AC AC 120 VAC to 9 VAC 0393503101 Transformer adapter AC AC 240 VAC to 9 VAC 039
20. oximately 0 8 kg 1 8 Ib Size Mixer module 4 2 x 7 5 x 8 8 cm 1 7 x 3 0 x 3 6 in AC adapter 6 0 x 5 05 x 4 1 cm 2 3 x 2 0 x 1 6 in Page 28 of 28 Publication no 0391467400 Issue 5 February 2009
21. ring inside the seal maintains contact with the mixer bore and the piston with enough force to seal at low pressures but not excessive force to prevent the seal from sliding as the mixer cap is hand tightened When pressurized mobile phase entering the mixer body presses the seal against the mixer bore and the piston with increased force The increased force maintains sealing action at HPLC pressures Caution Over tightening the cap with tools may damage the cap or body The inlet ports on the analytical and narrowbore mixers accept standard 10 32 fittings The outlet port uses an extra long 10 32 fitting On the preparative scale both inlet and outlet ports accept 1 4 28 fittings for 1 8 in tubing Effective sealing in Varian Dynamic mixers is a function of the passive sealing mechanism only The sealing action cannot be improved by tightening the cap with more force than can be applied by hand Publication no 0391467400 Issue 5 February 2009 Page 7 of 28 Varian Dual Chamber Dynamic Mixer This page is intentionally left blank Page 8 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 2 Installation 2 1 Unpacking Before opening the shipping container check for signs of damage or rough handling Report damage to the carrier and your local Varian sales service office 2 2 Plumbing 2 2 1 Binary Gradient HPLC Connections 1 7 5
22. se this frit may become clogged resulting in restricted flow through the mixer and HPLC system To check for restricted flow you will need a pressure monitor in the HPLC system between the pumps and the mixer To check for restricted flow 1 Open the prime purge valve so the flow is diverted to waste 2 Run the pumps with methanol rinsing first with water if necessary at a moderate flow rate 1 mL min and check the pressure monitor If the frit and the fluid lines are in good condition the pressure reading on the monitor should be minimal A reading over 689 5 kPa 100 psi indicates that the frit is probably clogged and needs to be replaced A clogged frit cannot be cleaned The frit can be replaced without fully dismantling the mixer or disconnecting the outlet tubing 1 Follow steps 1 2 and 3 in the procedure in Section 3 2 2 Loosen and remove the seal retainer includes frit 3 Thread the replacement seal retainer and frit onto the piston Finger tighten the seal retainer 4 Press the piston in the mixer body bore 5 Replace the mixer cap Page 22 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer 3 6 Seal Replacement If leaks are seen at the top of the mixer and the correct outlet fitting is swaged properly the seal may need to be replaced Caution Leaks from the top of the mixer cannot be prevented by tightening the mixer cap Tightening the mixer cap more t
23. sing non finger tight fittings Tighten using two open end wrenches as follows hold the piston steady with a wrench across the flats while you tighten the outlet fitting with another wrench Do not over tighten Reconnect the inlet lines to the base of the mixer body in the same manner 4 The mixer reassembly is now complete Connect the power cord and check that the LED comes on to show mixer operation 5 When the plumbing is complete operate the system to check for any leaks If leaks are found stop the pumps and tighten the leaky fittings just enough to stop the leak Warning Chemical Hazard Death serious injury or damage to skin can result from contact with chemicals used in HPLC especially when under high pressure Always wear appropriate protective equipment and clothing Caution A leak at the top of the mixer may be caused by a damaged seal In this case tightening the outlet fitting further will have no effect on the leak Over tightening the outlet fitting may damage the fitting or the piston If the seal is damaged replace it See Section 3 6 Publication no 0391467400 Issue 5 February 2009 Page 21 of 28 Varian Dual Chamber Dynamic Mixer 3 5 Checking and Replacing the Outlet Filter Frit Analytical and Narrowbore Mixers Only These mixers incorporate a 2 um frit pressed into the seal retainer to protect the downstream components of the HPLC system from particulate contamination With normal u
24. th a wrench When tightening the outlet fitting hold the top of the piston steady with another wrench 1 ME Sate Figure 4 Compression fittings for the 0 6 mL Narrowbore and 1 2 mL analytical stainless steel mixers Where 1 Fitting 2 Ferrule 2 3 2 1 2 mL PEEK 0 6 mL Titanium and 1 2 mL Titanium Mixers Slide the 1 16 in OD outlet tubing through the one piece fitting and ferrule and push the end of the tubing into the port as far as possible Holding the tubing in place finger tighten the fitting Do not use tools to tighten For both inlet ports place a fitting and ferrule on the tubing and tighten in the same manner as the outlet fitting Do not use tools to tighten Page 12 of 28 Publication no 0391467400 Issue 5 February 2009 Varian Dual Chamber Dynamic Mixer aaa Figure 5 1 2 mL Titanium Analytical Mixers Where 1 Extra long fitting and ferrule 2 Inlet fitting and ferrule 2 3 3 10 mL PEEK and Titanium mixers All ports are identical Slide the fitting and ferrule ferrule collar over the 1 8 in OD tubing as shown and push the end of the tubing into the port as far as possible While holding the tubing in place finger tighten the fitting When tightening the outlet port fitting hold the piston steady with another wrench Note When the system plumbing is complete check for any leaks If a leak is found stop the pumps and tighten the affected fitting just enough to stop the leak Fi
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