Model 6300 and 6320D Spectrophotometer
Contents
1. 14 3 2 SPECTROSCOPY MEASUREMENT There are four main components of a spectrophotometer These are a light source to emit a high and constant amount of energy over the full wavelength range a method for separating the light into discreet wavelengths a sample holder and a light detector The optical layout of the 6300 and 6320D spectrophotometers is shown below LAMP A SC dp ENTRANCE SLIT Es GRATING COLLIMATOR MIRROR nt A EXIT SLIT DETECTOR y AN L VU SAMPLE COLLECTING LENS Figure 3 2 1 Diagram of light path The light from the pre focused tungsten halogen lamp is focused onto the grating with 1200 lines per millimeter which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens arrangement before passing through the sample in the sample chamber from left to right The light which is not absorbed by the sample is transmitted through a collecting lens and onto the signal detector The photo diode detector used is mounted directly onto the detector PCB and is used to calculate the transmittance The result is displayed either as transmittance or absorbance on the instrument display 15 3 3 16 GOOD PRACTICE GUIDELINES 1 For optimum performance all spectrophotometers should be sited in a clean dry dust free atmosphere When in use ambient temperature and light levels should remain as constant as possible 2 I2. Operating Manual Bibby Scientific 630 026 REV G 06 14 Safety Please read this information carefully prior to installing or using this equipment 1 The 63 series spectrophotometers are designed for operation by trained personnel who are aware of the principles and applications involved For further help and advice please visit www jenway com 2 The spectrophotometer is a sensitive electronic and optical instrument designed for use in a laboratory environment Careful adherence to the installation instructions must be observed If in doubt contact a relevant and competent authority for advice before proceeding 3 In addition to observing the instructions detailed in the Operating Manual and Service Manual for this instrument all installation operating and service personnel must be aware of and employ a safe system of work 4 Voltage levels hazardous to life are present in this instrument for personal safety only trained engineers aware of the risk and avoidance of electric shock should remove protective covers from the instrument 5 This instrument is designed for minimal maintenance which must be carried out carefully following the procedures detailed in this manual All safety instructions in these procedures as well as those defined locally for the area or environment where the work is being carried out must be observed 6 Other than for those items defined in the maintenance procedures herein there are no user servicea
3. 2 6 EN LEE 13 SECTION 3 Theory and Practice of Spectroscopy Measurements 14 3 1 THEORY OF SPECTROSCOPY MEASUREMENT saciar ana 14 3 2 SPECTROSCOPY MEASUREMENT tee 15 3 3 GOOD PRACTICE GUIDELINES een 16 SECTION 4 PHOTOMETRICS MEAGUREMENTE 18 4 1 TRANSMISSION MEASUREMENT MODE ii 18 4 2 ABSORBANCE MEASUREMENT MODE eee 18 SECTION 5 CONCENTRATION MEASUREMENTS ii 20 5 1 USING A KNOWN STANDARD unsinnig 20 5 2 USINGAKNOWN IFACTOR datori ee 20 SECTION 6 CONNECTING TO A Piscinas 21 6 1 ENEE 22 6 2 Recorder Outputs an a a a eR E E E AR 23 SECTION 7 Accessories and Spare Parts 24 7 1 OPTIONALAGCCESSORIES nent matins en 24 7 2 ll 24 SECTION 8 Maintenance and Service nn 25 8 1 ROUTINE MAINTENANCE una 25 8 2 LAMP REPLACEMENT occitano Diao 25 8 2 1 Tungsten Halogen Lamp Replacement noc cc nan cnn nn 25 8 3 A e e EE O 26 SEGTON 9 Troubleshootld sii dd 27 9 1 ERROR CODES dde 27 9 2 TECHNICAL SUPPORT noire anali i 27 SECTION 10 Declaration of Conformity i 28 D TS O o 29 SECTION 1 Introduction 1 1 INSTRUMENT DESCRIPTION The 6300 and 6320D spectrophotometers are suited to a wide range of applications in education quality control environmental and clinical analysis Both models are visible spectrophotometers covering a wavelength range from 320nm to 1000nm with measurement modes for absorbance transmittance and concentration Thes
4. factors concentration and units The main menu screen and surrounding keypad is displayed below 11 SSA COS II GUERRE 2 CAL CH T ABS CONC xF UNITS Fig 2 4 1 Controls Arrow Keys Used to adjust values on the selected display Arrow Keys Used to move the cursor horizontally between menu options Return Key Used to select the display menu option CAL Key Initiates a calibration routine OI NT Print Key Provides a printout of the current reading with an incremental sample number When pressed for the first time after a calibration the print out will give calibration information The incremental sample number will be reset after a calibration 2 5 REAR PANEL The image below shows the rear panel on the instrument DANGER A C Disconnect power before MAI A removing covers Analogue Fig 2 5 1 Rear Panel 12 1 Lamp access panel Allows access to lamp when replacement is necessary NOTE The lamp access panel and all ventilation slots must NOT be covered or obstructed at any time 2 Power switch On off switch for the unit 3 Power in socket IEC type connection socket for mains cable 4 Output sockets Analogue output 5 RS232 serial port Connection to aPC 2 6 SAMPLING Model 6300 is supplied with a 10mm cuvette holder Other accessories can be fitted refer to section 7 Model 6320D is supplied with a dual 10mm cuvette holder wi
5. repair In this case please contact the Service Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Certificate This is available as a downloadable pdf file at www jenway com or contact us and we will be happy to send you a copy Please clearly mark the package for the attention of the Service Department and post to the following address Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 OSA United Kingdom All replacement parts are guaranteed for 1 year and where ever possible returned equipment is turned around in 10 working days 26 SECTION 9 Troubleshooting 9 1 ERROR CODES Refer to the table below to diagnose error codes If you require further assistance contact Jenway Help refer to section 9 2 Error Code Err 1 Err 2 Err 3 Err 4 Err 5 Err 6 Error Name Dark cal error Light cal error Concentration calibration error Lamp Failure No light detected No dark level detected Issue This error occurs when the 6300 6320D closes the shutter to block light entering the sample chamber If the detector output does not fall to a level normally associated with a dark cal then this error is indicated The most likely cause is that the sample chamber lid is not closed This error indicates that there is insufficient light to calibrate t
6. T units To adjust the wavelength use the UP and DOWN arrow keys The calibration must be performed at the same wavelength at which the sample will be measured Insert a cuvette containing the blank clear solution into the sample chamber and close the instrument lid Press the CAL key The instrument will perform a zero transmission calibration followed by a 100 transmission calibration An internal shutter is automatically activated to perform the zero setting and this part of the routine is therefore independent of the solution in the light path Once the calibration has been performed a sample can be measured Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and the transmittance result will be shown on the screen Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured 4 2 ABSORBANCE MEASUREMENT MODE To select absorbance use the LEFT or RIGHT arrow keys to move the cursor to the ABS indicator on the menu bar refer to figure 2 3 1 The primary display will show the absorbance with ABS units To adjust the wavelength use the UP and DOWN arrow keys The calibration must be performed at tne same wavelength at which the sample will be measured Insert a cuvette containing the blank clear
7. index mark set to the correct position before measurements are made f Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold Plastic cuvettes are not compatible with organic solvents g All sample containers must be handled with care by the top bottom and non optical surfaces only Any finger marks evident must be removed by a suitable cleaning process 10 11 h Flow through cuvettes must be selected with care and consideration for the sample type sample volume pumping system rinse sample and waste handling to be used Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible If stored in stoppered and sealed cuvettes they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material Samples should be allowed to equilibrate to ambient temperature before measurement unless a suitable temperature controlled sample holder is in use Temperature change during measurement may cause air bubbles to form on the walls of the sample holder This is acommon cause of drift during measurement In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable so
8. personnel non qualifi annuleront toute garantie et entra neront des frais suppl mentaires de r paration 7 Il est n cessaire de toujours consulter les donn es relatives la sant et la s curit de chaque produit chimique ou r actif utilis Tous les conseils informations et avertissements disponibles concernant la manipulation le stockage l utilisation et l limination de ces produits chimiques doivent tre respect s soigneusement Lorsqu elles ne sont pas disponibles ces donn es doivent tre demand es dans tous les cas au fournisseur avant de poursuivre 8 Il est important que de bonnes pratiques de laboratoire soient respect es lors de la manipulation d chantillons de produits chimiques de r actifs et d quipements auxiliaires afin de r aliser des mesures et des analyses avec cet instrument Des quipements de protection individuels et de s curit adapt s doivent tre utilis s tout moment 9 Si une protection de s curit est susceptible d tre d t rior e le spectrophotom tre doit tre mis hors service et s curis pour viter toute utilisation pr vue L tat d fectueux doit tre signal l autorit en charge de la maintenance Le num ro du mod le et le num ro de s rie du spectrophotom tre doivent tre indiqu s dans tous les rapports Sicherheit Dies sind wichtige Informationen bitte lesen Sie sie vor Installation oder Verwendung dieses Ger ts sorgf ltig durch 1 Die
9. the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured 5 2 USING A KNOWN FACTOR If the factor is known there is no need to calibrate against a known standard To enter the factor use the LEFT or RIGHT arrow keys to move the cursor to the xF indicator on the menu bar refer to figure 2 3 1 Use the UP and DOWN arrow keys to enter the required factor Once the required factor has been entered press the RETURN or CAL key to store the factor and return to the concentration measurement mode 20 SECTION 6 CONNECTING TO A PC Connect the interface cable to the RS232 serial port on the rear of the instrument and connect to the serial port on the rear of the PC Turn the PC on and load the PC software by inserting the PC software disc into the CD drive If the PC software does not auto run open My Computer and double click on the 63 Zero software icon Follow the instructions to install the PC software to the required location Once the software is installed turn the mains power on to the instrument The PC software is pre configured to run using the following settings 1200 baud 7 data bits Odd parity 1 stop bit The 25 way D connector allows a standard one to one interconnection lead to be used as supplied with the 40 column printer interface cable kit A printout is initiated by pressing the PRINT key If the sample number is unity then the printout
10. 85 813748 e mail info bibby scientific com www bibby scientific com Bibby Scientific Middle East Ltd BPO Box 27842 Engomi 2433 Nicosia Cyprus Tel 357 22 660 423 Fax 357 22 660 424 hail sales bibby scientificme com www bibby scientific com Bibby Scientific France SAS ZI du Rocher Vert BP 79 77793 Nemours Cedex France Tel 33 1 64 45 13 13 Fax 33 1 64 45 13 00 e mail bsf bibby scientific fr www bibby scientific com Bibby Scientific US Ltd 3 Terri Lane Suite 10 Burlington NJ 08016 USA Tel 800 225 9243 Fax 609 589 2571 www bibby scientific com Bibby Scientific Ltd Singapore Prudential Tower Level 26 30 Cecil Street 049712 Singapore Tel 65 6631 2976 Fax 44 0 1785 810405 e mail bibby bibby scientificasia com www bibby scientific com
11. DCD To PC 4 DTR From PC 5 GND 6 2 Recorder Output This is available via the 4mm rear panel sockets The level is proportional to the displayed reading depending on the measurement mode Transmission Absorbance Concentration 1mV per 0 1 T 1mV per 0 001ABS 1mV per concentration unit 23 SECTION 7 Accessories and Spare Parts 7 1 OPTIONAL ACCESSORIES Part Code 634 001 632 001 632 031 630 204 637 071 630 005 630 304 648 001 632 511 033 290 630 028 037 551 543 001 060 287 060 288 542 009 7 2 SPARES Part Code 012 075 630 204 632 511 060 084 HH179 S HH180 S FCABLEUS 635 100 013 210 016 021 016 025 Description of Accessory 4 position manual cuvette holder Sipper pump supplied with inlet and outlet tubing 230V 50Hz Sipper pump supplied with inlet and outlet tubing 110V 60Hz 10x10mm path length cuvette holder 10mm cuvette and 16 24mm test tube holder 10 to 100mm adjustable path length cuvette holder Micro cuvette holder Water heated 10x10mm single cuvette holder Dual cell holder for 10mm cuvettes and 12 7mm diameter tubes 6320D ONLY Storage carry case not for use with 6320D Dust cover RS232 to USB converter for use with computer without a serial port 40 Column printer supplied with interface cable paper roll and ribbon and universal power supply Paper roll for printer quantity 1 Printer ribbon quantity 1 Interface cable kit Description of Spare Part T
12. HT arrow keys to move the cursor to the UNITS indicator on the menu bar refer to figure 2 3 1 Use the UP and DOWN arrow keys to select ppm mg l g l M or blank units Once the required units have been selected press the RETURN or CAL key to store the units and return to the concentration measurement mode 5 1 USING A KNOWN STANDARD The calibration must be performed at the same wavelength at which the sample will be measured Insert a cuvette containing the known concentration standard into the sample chamber and close the instrument lid Press the CAL key the CAL annunciator will flash continuously Use the arrow keys to change the concentration to the required value for the standard Press the CAL or RETURN key to calibrate the instrument to the standard concentration The instrument will calculate the factor for subsequent measurements lf the factor calculated is out of range then the instrument will display Err 3 for 2 seconds after the calibration To view the factor use the LEFT or RIGHT arrow keys to move the cursor to the xF indicator on the menu bar refer to figure 2 3 1 Once the calibration has been performed a sample of unknown concentration can be measured Remove the cuvette containing the standard solution and place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and the concentration result will be shown on the screen Subsequent samples can be measured in the same way If
13. Spektrophotometer der 63 Serie sind f r den Betrieb durch geschultes Personal das ber die entsprechenden Prinzipien und Anwendungen Bescheid wei vorgesehen Weitere Hilfe und Hinweise finden Sie auf www jenway equipment de 2 Ein Spektrophotometer ist ein empfindliches elektronisches und optisches Instrument das f r die Verwendung in Laboren vorgesehen ist Die Installationsanweisungen sind genau zu beachten Wenden Sie sich im Zweifelsfall an die jeweils zust ndige Stelle bevor Sie fortfahren 3 Neben der Befolgung der Anweisungen des Betriebshandbuchs und des Wartungshandbuchs f r dieses Ger t muss s mtliches Installations Betriebs und Wartungspersonal ber ein sicheres Arbeitssystem informiert sein und dieses anwenden 4 In diesem Ger t herrschen lebensgef hrliche Spannungspegel aus Sicherheitsgr nden d rfen nur geschulte Techniker die ber die Gefahr und Vermeidung von Stromschl gen informiert sind Schutzabdeckungen vom Ger t entfernen 5 Dieses Ger t ist f r minimale Wartungsarbeiten ausgelegt die unter Einhaltung der in diesem Handbuch dargelegten Verfahren sorgf ltig durchzuf hren sind Alle Sicherheitsanweisungen dieser Verfahren sowie die rtlich festgelegten Anweisungen f r das Gebiet oder Umfeld in dem die Arbeit ausgef hrt wird sind einzuhalten 6 Au er der in den Wartungsverfahren in diesem Handbuch angegebenen Teile gibt es in diesem Instrument keine Teile die vom Benutzer zu warten sind Di
14. ara ser utilizados por personal con formaci n que conozca los principios y las aplicaciones que entra an Si necesita m s ayuda o indicaciones dir jase a www jenway com 2 El espectr metro es un instrumento electr nico y ptico de gran sensibilidad destinado a entornos de laboratorio Deben seguirse atentamente las instrucciones de instalaci n En caso de duda consulte a la autoridad competente y pertinente antes de continuar 3 Aparte de cumplir las instrucciones detalladas en el Manual de funcionamiento y el Manual de servicio de este instrumento todo el personal de instalaci n manejo y servicio debe conocer y aplicar un sistema seguro de trabajo 4 Este instrumento presenta niveles de tensi n peligrosos por motivos de seguridad personal las tapas protectoras del instrumento debe ser retiradas nicamente por t cnicos debidamente cualificados que conozcan los riesgos y sepan evitar las descargas el ctricas 5 Este instrumento ha sido dise ado con requisitos m nimos de mantenimiento que debe realizarse con cuidado de acuerdo con los procedimientos detallados en este manual Deben cumplirse todas las instrucciones de seguridad de estos procedimientos as como las 5 que se establezcan localmente para la zona o el entorno donde se est realizando el trabajo 6 A excepci n de los elementos indicados en los procedimientos de mantenimiento de este manual este instrumento no contiene piezas que necesiten ning n tipo d
15. ble items in this instrument Removal of covers and attempted adjustment or service by unqualified personnel will invalidate any warranty and incur additional charges for repair 7 Reference should always be made to the Health and Safety Data for any chemicals or reagents used All available information advice and warnings on the handling storage use and disposal of such must be carefully observed When not available this data must be requested from the supplier before proceeding in any way 8 Itis important that good laboratory practice is observed when handling samples chemicals reagents and ancillary equipment in order to carry out measurement and analysis with this instrument Suitable safety and personal protective equipment must be used at all times 9 If it is suspected that safety protection has been impaired in any way the spectrophotometer must be made inoperative and secured against any intended operation The fault condition must be reported to the appropriate servicing authority In all such reports the model number and serial number of the spectrophotometer must be quoted S curit Ces informations sont importantes veuillez les lire avec attention avant d installer ou d utiliser l instrument 1 Les spectrophotom tres de la s rie 63 sont congus pour tre utilis s par du personnel form qui conna t les principes et les applications concern s Pour obtenir de l aide et des conseils suppl mentaires merci de consul
16. e Entfernung von Abdeckungen sowie der Versuch der Einstellung oder Wartung durch unqualifiziertes Personal f hren dazu dass die Garantie ung ltig wird und k nnen zus tzliche Reparaturkosten nach sich ziehen 7 Die Angaben zum Gesundheitsschutz und zur Arbeitssicherheit f r die verwendeten Chemikalien bzw Reagenzien sind jederzeit zu ber cksichtigen S mtliche verf gbaren Informationen Hinweise und Warnungen in Bezug auf die Handhabung Lagerung Verwendung und Entsorgung dieser Stoffe sind genau zu befolgen Falls diese Informationen nicht verf gbar sind m ssen sie vom Lieferanten angefordert werden bevor die Arbeiten fortgesetzt werden 8 Es ist wichtig dass bei der Handhabung von Proben Chemikalien Reagenzien und Hilfsger ten zur Durchf hrung von Messungen und Analysen mit diesem Instrument die gute Laborpraxis befolgt wird Es ist jederzeit eine geeignete Sicherheitsausr stung und pers nliche Schutzausr stung zu verwenden 9 Falls vermutet wird dass der Sicherheitsschutz auf irgendeine Weise beeintr chtigt wurde ist das Spektrophotometer au er Betrieb zu setzen und gegen den Betrieb zu sichern Die St rung ist der entsprechenden Wartungsstelle zu melden In diesen Berichten ist die Modellnummer und die Seriennummer des Spektrophotometers anzugeben Sequridad Informaci n importante que debe leer atentamente antes de instalar o utilizar este instrumento 1 Los espectr metros de la serie 63 se han concebido p
17. e fuse holder Replace the fuse retainer in its holder fit the correct fuse and push assembly back into the power input socket For further information please refer to the service manual Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument DO NOT open the sample chamber door during initialisation 10 The instrument will automatically re align the monochromator at zero order wavelength During this initialisation the instrument displays CAL on the primary display before displaying the main measurement screen Please note that the instrument will go to the last wavelength and measurement mode used 2 3 2 4 ON Oo e De DISPLAY These spectrophotometers have an LCD display 4 3 5 6 7 T ABS CONC xF UNITS A A A 8 Fig 2 3 1 Display Primary display area Transmission Absorbance Concentration Primary display adjust annunciator Secondary display area Wavelength Factor Primary display units Secondary display annunciator Operation with PC Menu options T ABS CONC FACTOR UNITS Menu pointers for above CONTROLS The keypad used for these models enables an easy and effective way of navigating the different measurement modes The arrow keys can be used to move between modes and to adjust wavelengths
18. e mantenimiento por el usuario La retirada de las tapas y cualquier intento de ajuste o mantenimiento por parte de personal no cualificado dar lugar a la invalidaci n de la garant a y podr a causar gastos adicionales de reparaci n 7 Deben consultarse siempre los datos sobre salud y seguridad de los productos qu micos o reactivos empleados Debe leerse atentamente toda la informaci n los consejos y las advertencias existentes sobre la manipulaci n el almacenamiento el uso y la eliminaci n de los mismos Si no se dispone de informaci n deber solicitarse al proveedor antes de continuar 8 Es importante adoptar buenas pr cticas de laboratorio cuando se manipulen muestras productos qu micos reactivos y equipos auxiliares para realizar medidas y an lisis con este instrumento Se deben utilizar siempre equipos de seguridad y protecci n personal 9 Ante la sospecha de que alguna medida de seguridad se haya visto de alg n modo comprometida el espectr metro deber dejarse inutilizado as como garantizarse que nadie pueda utilizarlo Debe informarse de cualquier fallo al responsable de servicio pertinente En todos los informes deben indicarse el n mero de modelo y el n mero de serie Sicurezza Queste informazioni sono importanti si prega di leggerle attentamente prima di installare o utilizzare lo strumento 1 Gli spettrofotometri della serie 63 sono progettati per essere utilizzati da personale qualificato che con
19. e models have full interfacing capability for Analogue output and serial RS232 interfacing The optical system is independently housed and isolated with lenses to give maximum protection from environmental contamination Combined with a mechanically rigid structure these models provide a system with fast warm up low drift and high reliability 1 2 INSTRUMENT SPECIFICATION 6300 and 6320D Wavelength Range 320 to 1000nm Resolution 1nm Accuracy 2nm Spectral bandwidth 8nm Photometrics Transmittance 0 to 199 9 Absorbance 0 300 to 1 999A Accuracy 1 T 0 01Abs at 1 000 Absorbance Resolution 0 1 T 0 001A Stability 1 hr after 15 minute warm up Concentration Range 300 to 1999 Resolution 1 0 1 Calibration Blank with a single standard or factor Units no units ppm M g l mg l Factor 0 1 to 9999 Standard 0 1 to 1999 Other Beam height 15mm Light source Tungsten halogen lamp Outputs Analogue RS232 Power lt 50W Size wxdxh 365 x 272 x 160mm Weight 6kg SECTION 2 Installation 2 1 UNPACKING Remove the 6300 or 6320D from the packaging and ensure the following items are included 1 Model 6300 spectrophotometer or Model 6320D spectrophotometer 2 Required power cable either UK mains lead moulded plug HH179 S EU mains lead moulded plug HH180 S or US mains lead moulded plug FCABLEUS 8 Pack of 100 di
20. f required adherence to Standard Operating Procedures SOP and Good Laboratory Practice GLP should be monitored with regular calibration checks and a suitable Quality Control QC programme 3 The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed 4 The correct selection of sample containers is imperative for accurate and reproducible results a Check that the material of the sample container is compatible with the wavelengths to be used for measurement In general glass can only be used down to 360nm or 320nm depending on quality Standard plastic cuvettes can be used down to 320nm Special UV versions can be used down to 260nm Below this level quartz cuvettes must be used b Plastic disposable cuvettes should only be used ONCE c Glass cuvettes should be thoroughly cleaned after use Discard when scratches become evident on optical surfaces d Care should be taken when selecting semi micro or micro cuvettes The cuvette window on the inner chamber the area filled with sample must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample In this case semi micro or micro cuvettes with self screening black surrounds must be used or alternative holders for these cuvettes should be used e Glass test tubes and other sample tubes should be used with care Where possible matched tubes should be used and any
21. lvents must be used for dissolving or diluting samples chemicals and reagents All measurements require calibration to a blank for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample Where reagents are added to the sample to produce a colour proportional to its concentration a sample based blank should be used In this case the blank should consist of all reagents or chemicals to be used except the sample which will produce the colour to be measured Deviations from the Beer Lambert Law may occur at high and low concentrations giving non linear response during sample concentration measurements For all new methods a linear range should be defined by the preparation of a calibration curve Cuvettes and sample holders must be filled to a minimum level which covers the light path All Jenway spectrophotometers have a beam height of 15mm The instrument must be calibrated to zero absorbance 100 transmittance prior to taking readings 17 SECTION 4 PHOTOMETRICS MEASUREMENTS The 6300 6320D can perform simple measurements of transmittance or absorbance The sample is measured at one wavelength and at one point in time 4 1 TRANSMISSION MEASUREMENT MODE To select Transmittance use the LEFT or RIGHT arrow keys to move the cursor to the T indicator on the menu bar refer to figure 2 3 1 The primary display will show the transmittance with
22. mal measurements ASCII Gnnn lt CR gt Commands the instrument to go to wavelength nnm For example G540 lt CR gt will set the wavelength to 540nm ASCII Fxxxx x lt CR gt Sets the concentration factor to xxxx x For example F1000 lt CR gt will set the factor to 1000 Note lt CR gt is an ASCII carriage return character The last three commands provide an output which can be readily incorporated into most spreadsheet software packages 6 1 RS232 Output The bi directional RS232 interface is available on the rear panel 25 way D type connector The connections are as follows TDX 2 INPUT TO 6300 6320D RXD 3 OUTPUT FROM 6300 6320D RTS 4 LINKED TO CTS CTS 5 LINKED TO RTS DSR 6 OUTPUT FROM 6300 6320D DCD 8 OUTPUT FROM 6300 6320D DTR 20 INPUT TO 6300 6320D must be active GND 7 Suggested interconnections are detailed below 6300 6320D IBM PC XT 25 way D TDX2 2 TXD From PC RXD 3 3 RXD To PC RTS4 4 RTS From PC CTS5 5CTS To PC DSR6___ 6 DSR To PC DCD 8 8 DCD To PC DTR 20 20 DTR From PC GND 7 7GND 22 6300 6320D TDX 2 RXD 3 RTS 4 CTS 5 DSR 6 DCD 8 DTR 20 GND 7 Note the interface cable kit 542 009 can be used to implement the above interconnections IBM PC XT 9 way D 3 TXD From PC 2 RXD To PC 7 RTS From PC 8 CTS To PC 6 DSR To PC 1
23. nce with the requirements of these Directives is claimed by meeting the following standards EN 61326 1 1998 Electrical Equipment for Measurement Control and Laboratory use EN 61010 1 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory use CE mark gt Signed 2 Mr S Marriott Date K A 14 Authority Technical Director Bibby Scientific Ltd Bibby Scientific Ltd Stone Staffs ST15 OSA UK Bibby Scientific j Tel 44 0 1785 812121 Fax 44 0 1785 813748 28 Declaration of Conformity L ee PE ra a A min ne A DESIO EE Visible Spectrophotometer Model 6320D This product complies with the requirements of the EU Directives listed below 89 336 EEC EMC Directive 73 23 EEC Low voltage Directive LVD Compliance with the requirements of these Directives is claimed by meeting the following standards EN 61326 1 1998 Electrical Equipment for Measurement Control and Laboratory use EN 61010 1 2001 Safety Requirements Electrical Equipment for Measurement Control and Laboratory use CE mark affi Signed Mr S Marriott gt 3 2 14 Authority Technical Director Bibby Scientific Ltd Bibby Scientific Ltd Stone Staffs ST15 OSA UK Bibby Scientific j Tel 44 0 1785 812121 Fax 44 0 1785 813748 29 Bibby Scientific Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 OSA United Kingdom Tel 44 0 1785 812121 Fax 44 0 17
24. o 100 The most likely cause is that the light at the selected wavelength is being absorbed by a sample in the sample chamber This error can also be caused if the lamp has failed The calculated factor is outside the instrument range Recalibrate the instrument using a standard of lower concentration The lamp failed after initially finding Onm peak If the lamp has failed it should be replaced The instrument has failed to find a zero order peak Possible causes of this error are that the lamp has failed or that a sample has been left in the unit which is absorbing the zero order light The instrument checks the region below 320nm which should have no optical throughput If this region cannot be found the most likely cause is that the sample chamber lid is not closed 9 2 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help with any applications advice and questions you may have about our products and how to use them If you require any technical or application assistance please contact the team at E mail jenwayhelp bibby scientific com Phone 44 0 1785 810433 27 SECTION 10 Declaration of Conformity Declaration of Conformity LR A EA n ee gl Visible Spectrophotometer Model 6300 This product complies with the requirements of the EU Directives listed below 89 336 EEC EMC Directive 73 23 EEC Low voltage Directive LVD Complia
25. olazione o riparazione da parte di personale non qualificato renderanno nulla qualsiasi garanzia e genereranno dei costi aggiuntivi per la riparazione 7 Fare sempre riferimento ai dati sulla salute e la sicurezza per tutte le sostanze chimiche o reagenti utilizzati Osservare attentamente tutte le informazioni i consigli e le avvertenze disponibili sulla loro manipolazione conservazione utilizzo e smaltimento Qualora tali dati non siano disponibili richiederli al fornitore prima di procedere in qualsiasi modo 8 importante che vengano osservate le buone pratiche di laboratorio quando si manipolano campioni sostanze chimiche reagenti e apparecchiature accessorie per eseguire misurazioni e analisi con questo strumento Attrezzature idonee per la protezione personale e la sicurezza vanno utilizzate in ogni circostanza 9 Se si sospetta che la protezione della sicurezza sia stata compromessa in qualsiasi modo disattivare e rendere inutilizzabile lo spettrofotometro La condizione di guasto deve essere comunicata al responsabile della manutenzione appropriato Tale comunicazione deve sempre comprendere il numero di modello e il numero di serie dello spettrofotometro Contents SECTION REN Ta ee ele EE 9 1 1 INSTRUMENT DESCRIPTION asus ladies 9 1 2 INSTRUMENT SPECIFICATI N asus and 9 SEGTIION 2 Installation oe 10 2 1 UNPACKING oia ee 10 2 2 SEKR E Ne EE 10 2 3 DISPLAY ia o rr E 11 2 4 CONTROLS paria tada aida 11 2 5 ll EE 12
26. osce i principi e le applicazioni ad essi collegati Per ulteriori informazioni e per assistenza visitare il sito internet www jenway com 2 Lo spettrofotometro uno strumento ottico elettronico sensibile progettato per l uso in laboratorio Seguire minuziosamente le istruzioni di installazione In caso di dubbi rivolgersi all autorit competente appropriata per una consulenza prima di procedere 3 Oltre ad osservare le istruzioni indicate nel Manuale di funzionamento e manutenzione del presente strumento tutto il personale addetto all installazione funzionamento e manutenzione deve conoscere e implementare un sistema di lavoro sicuro 4 Questo strumento utilizza livelli di tensione pericolosi pertanto per la sicurezza personale solo i tecnici qualificati che conoscono i rischi e come evitare le scosse elettriche possono rimuovere le coperture protettive dallo strumento 5 Questo strumento progettato per necessitare di una manutenzione minima che per deve essere eseguita con cura seguendo le procedure indicate in questo manuale Rispettare tutte le istruzioni per la sicurezza presenti in queste procedure nonch quelle definite a livello locale per l area o l ambiente in cui viene eseguito il lavoro 6 Ad esclusione delle parti indicate nelle procedure di manutenzione contenute in questo manuale l apparecchiatura non contiene componenti che possano essere riparati dall utente La rimozione di coperture ed eventuali tentativi di reg
27. ply and ensure the lamp is cool before handling Care should be taken when removing the lamp from the holder Access to the tungsten halogen lamp can be gained via the lamp access panel located on the rear of the instrument refer to section 2 5 1 Slacken off the thumbscrew from the lamp access panel located at the rear of the unit 2 Remove the old lamp from the holder The lamp is a plug in fit and should be removed by gently easing it from the holder 3 Carefully remove the replacement lamp from the packaging Ensure that the glass portion of the lamp is not touched as finger marks will damage the lamp resulting in a reduced performance If accidental damage occurs the surface of the lamp may be cleaned using propan 2 ol 4 Insert the lamp into the holder ensuring it is fully pushed in 25 5 Replace the lamp access panel and re tighten the thumbscrew 6 Reconnect the power supply turn on the unit and ensure that the lamp is illuminated after a few seconds For further instructions refer to the service manual 8 3 SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Jenway equipment develops a fault Please contact them by one of the following means with a clear description of the problem E mail service bibby scientific com Tel 44 0 1785 810475 Fax 44 0 1785 810471 On occasion it may be necessary for your equipment to be sent back to our Service Department for
28. solution into the sample chamber and close the instrument lid Press the CAL key The instrument will perform a zero transmission calibration followed by a 0 000 Absorbance calibration An internal shutter is automatically activated to perform the zero setting and this part of the routine is therefore independent of the solution in the light path Once the calibration has been performed a sample can be measured Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and the absorbance result will be shown on the screen Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be 18 measured 19 SECTION 5 CONCENTRATION MEASUREMENTS The concentration measurement mode enables simple measurements of concentration to be performed In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor The sample is measured at one wavelength at one point in time To select concentration use the LEFT or RIGHT arrow keys to move the cursor to the CONC indicator on the menu bar refer to figure 2 3 1 The primary display will show the concentration with the selected units To adjust the wavelength use the UP and DOWN arrow keys To select the units use the LEFT or RIG
29. sposable plastic visible wavelength cuvettes 060 084 model 6300 only 4 Jenway 63 zero PC software 635 100 and interface cable 013 210 model 6300 only 5 Instruction manual 630 026 6 Jenway Foreign Manual CD JENMANCD 7 Optional accessories as ordered 2 2 INSTALLATION Models 6300 and 6320D are supplied ready to use The unit should be placed on a clean flat surface which is free from drafts and vibrations The units are designed for operation on 115V to 230V AC input at 50 to 60Hz The standard 2 metre cable supplied with the units is fitted with an IEC type connector which can be plugged directly into the POWER IN socket on the rear panel The unit should be positioned within 1 5 metres of an earthed mains supply The mains fuse is housed within the POWER IN socket When replacing the fuse the unit must be disconnected from the mains supply The fuse rating is 2A F fast blow type In the event of the fuse failing after replacement it is advisable to consult with Bibby Scientific Service or your distributor before proceeding further It is possible to switch the voltage between 230V and 115V When changing the voltage please ensure the fuse rating is correct Before attempting to change the voltage disconnect the instrument from the mains supply Withdraw the fuse holder from the power input socket and remove the fuse Extract the grey fuse retainer and rotate so that the correct voltage is visible through the aperture in th
30. ter le site Internet www jenway fr 2 Le spectrophotom tre est un instrument lectronique et optique sensible con u pour tre utilis en laboratoire Les instructions d installation doivent tre scrupuleusement respect es 3 En cas de doute contactez une personne comp tente afin d obtenir des conseils avant de proc der l installation 3 En plus de suivre les instructions d taill es des manuels d utilisation et d entretien de cet instrument tout le personnel impliqu dans l installation le fonctionnement et l entretien doit conna tre et utiliser un syst me de travail s r 4 Des niveaux de tension risque sont pr sents dans cet instrument Pour la s curit du personnel seuls des ing nieurs form s conscients du risque et de la n cessit d viter les chocs lectriques peuvent enlever les capots de protection de l instrument 5 Cet instrument est con u pour une maintenance minimale qui doit tre effectu e avec soin en suivant les proc dures d taill es dans ce manuel Toutes les instructions de s curit list es dans ces proc dures ainsi que celles d finies localement pour la zone ou l environnement dans lequel le travail doit tre r alis doivent tre respect es 6 Hormis les l ments d finis dans les proc dures de maintenance il n existe aucun l ment dans cet instrument qui peut tre r par par l utilisateur L enl vement des capots et les tentatives de r glage ou d entretien par du
31. th a 12 7mm test tube holder The domed lid on this model enables tall test tubes up to 100mm in height to be used WARNING The optical characteristics and light path of the cuvettes and test tubes used in the dual purpose sample holder vary so cannot be interchanged Calibrate on one and use only the same type of sample holder for each analysis batch 13 SECTION 3 Theory and Practice of Spectroscopy Measurements 3 1 THEORY OF SPECTROSCOPY MEASUREMENT UV visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample This is used to identify the presence and concentration of molecular entities within the sample The Beer Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through The Beer Lambert law states that A elc is the absorbance A is the molar absorption coefficient I mol om c is the concentration mol I I is the path length cm This law shows that absorbance is linear to concentration but this is only true for low concentrations For absorbance levels above 3 the concentration starts to move away from the linear relationship Transmittance is the proportion of the light which passes through the sample Where lo I lo is the incident light li is the transmitted light L is the path length gt Therefore T h lo Absorbance is inversely related to transmittance A log 1 T
32. ungsten halogen lamp 6300 amp 6320D 10x10mm path length cuvette holder Dual cell holder for 10mm cuvettes and 12 7mm diameter tubes 6320D ONLY Pack of 100 disposable plastic visible wavelength 10x10 cuvettes UK mains lead moulded plug EU mains lead moulded plug US mains lead moulded plug PC software on CD ROM Serial Lead to connect to PC Replacement fuse 2A Replacement fuse 1A To order spares please contact your local distributor or Bibby Scientific E mail spares bibby scientific com Tel 44 0 1785 810234 24 SECTION 8 Maintenance and Service 81 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust The sample area should always be kept clean and any accidental spillage should be wiped away immediately To give added protection when not in use the unit should be disconnected from the mains supply and covered with the optional dust cover The only routine maintenance which maybe required is the replacement of the light source The replacement lamp is available from your local distributor or Bibby Scientific refer to section 7 2 for spare part codes Only genuine replacement lamps should be used Similar lamps may have different filament configurations or be wavelength restricted for domestic or commercial use and will give rise to errors if used 8 2 LAMP REPLACEMENT 8 2 1 Tungsten Halogen Lamp Replacement Before replacing the lamp disconnect the unit from the mains sup
33. will include a header block The sample number is incremented every time the PRINT key is pressed The following commands can also be sent to the 6300 and 6320D via the serial interface using Windows HyperTerminal for example ASCII D or d Same as pressing the PRINT key ASCII T lt CR gt Outputs transmission and wavelength separated by an ASCII TAB character regardless of the instrument operating mode For example 100 0 540 ASCII A lt CR gt Outputs absorbance and wavelength separated by an ASCII TAB character regardless of the instrument operating mode For example 0 001 540 ASCII C lt CR gt Outputs concentration and wavelength separated by an ASCII TAB character regardless of the instrument operating mode For example 123 4 540 ASCII V lt CR gt Outputs a voltage proportional to the monochromatic light level passing the sample and wavelength separated by an ASCII TAB character For example 1234 5 540 ASCII Z lt CR gt Calibrates a zero absorbance if the dark shutter is open SO lt CR gt command or zero transmittance if the dark shutter is closed SC lt CR gt command ASCII SC lt CR gt Closes the dark shutter which blocks monochromatic light entering the sample chamber This allows 0 transmittance to be calibrated ASCII SO lt CR gt Opens the dark shutter which allows monochromatic light to enter the sample chamber This allows 100 transmittance zero absorbance to be 21 calibrated The shutter must be open for nor
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