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GP50 Gradient Pump Operator`s Manual

1. C 20 C 2 7 Diagnostic Test C 21 C 2 8 Pressure Statistics C 23 C 2 9 DSP Code Version C 24 C 3 Calibration Screens C 25 C 3 1 Calibration Menu C 25 C 3 2 Calibration Status C 26 C 3 3 Leak Sensor Calibration and Status C 27 C 3 4 Degas Status C 28 C 3 5 Flow Calibration C 29 C 3 6 Pressure Calibration C 30 C 3 7 Degas Pump Calibration C 34 D TTL and Relay Control D 1 TTL and Relay Output Operation D 2 D 2 TTL Input Operation D 3 D 2 1 TTL Input Signal Modes D 3 D 3 T
2. 3 2 3 1 3 Pressurize Eluent Reservoirs 3 2 3 1 4 Start Up 3 3 3 1 5 Selecting the Pressure Limits 3 4 3 1 6 Selecting the Operating and Control Modes 3 5 3 2 Running Under Direct Control Local Mode 3 6 3 3 Running Under Method Control Local Mode 3 7 3 3 1 Creating a New Method 3 8 3 3 2 Running a Method 3 10 3 3 3 Editing a Method 3 11 3 3 4 Deleting a Method 3 12 3 3 5 Changing the Running Method 3 12 3 3 6 Controlling the Method Clock 3 12 Contents Doc 031377 03 7 01 iii 3 4 Example Methods 3 13 3 4 1 Isocratic Method Example 3 13 3 4 2 Linear Gradient Method Example
3. 4 11 4 10 Poor Chromatographic Reproducibility 4 12 GP50 Gradient Pump iv Doc 031377 03 7 01 5 Service 5 1 Cleaning and Replacing the Check Valves 5 1 5 2 Piston Seal Replacement 5 4 5 3 Pump Piston Replacement 5 8 5 4 Pressure Transducer Waste Valve O Ring Replacement 5 10 5 5 Proportioning Valve Replacement 5 11 5 6 Changing Main Power Fuses 5 13 A Specifications A 1 Electrical A 1 A 2 Environmental A 1 A 3 Physical A 1 A 4 Display and Keypad A 1 A 5 Hydraulics A 2 A 6 Control
4. 2 14 2 4 4 Pump Priming Block 2 15 2 4 5 Pressure Transducer 2 15 2 5 Vacuum Degas Pump Assembly Optional 2 16 2 6 Piston Seal Wash Optional 2 16 2 7 Eluent Reservoirs 2 17 GP50 Gradient Pump ii Doc 031377 03 7 01 2 8 Rear Panel 2 18 2 9 Functional Description 2 18 2 9 1 Operating and Control Modes 2 18 2 9 2 Local and Remote Modes 2 19 2 9 3 Method Control 2 20 2 9 4 Eluent Delivery 2 22 3 Operation and Maintenance 3 1 Getting Ready to Run 3 1 3 1 1 Degas Eluents 3 1 3 1 2 Filter Eluents
5. 3 14 3 4 3 Curved Gradient Method Example 3 19 3 4 4 Editing a Running Method Example 3 23 3 5 Routine Maintenance 3 25 3 5 1 Daily Maintenance 3 25 3 5 2 Periodic Maintenance 3 27 3 6 Shutdown 3 27 4 Troubleshooting 4 1 Left Right Pump Head Pressure Fluctuations 4 1 4 2 Pump Will Not Start 4 3 4 3 Pump Stops 4 3 4 4 Liquid Leaks Leak Alarm 4 7 4 5 Noisy Pump Motor 4 8 4 6 Vacuum Degas Pump Does Not Run 4 9 4 7 Vacuum Degas Pump Calibration Fails 4 10 4 8 Vacuum Degas Pump Low Vacuum 4 11 4 9 Relays or TTLs Inoperative
6. GP50 Gradient Pump 3 2 Doc 031377 03 7 01 3 1 2 Filter Eluents Always filter eluents before beginning operation Filtering removes small particulates that may contaminate the eluent proportioning valves or the pump check valves and cause erratic flow rates or loss of prime The pressurizable reservoir Ship Kits supply end line filters P N 045987 for this purpose Install an end line filter on the end of each eluent line inside the reservoir To prevent air from being drawn through the lines make sure that the end of each filter reaches the bottom of the eluent reservoir and that each filter is submerged in eluent 3 1 3 Pressurize Eluent Reservoirs The GP50 Gradient Pump is capable of operation with or without head pressure on the eluent Pressurization of the eluent reservoirs if used should be with filtered inert gas preferably helium Refer to the Pressurizable Reservoir Installation Instructions for details 1 Verify that a regulator P N 046594 is installed on the gas supply line to the reservoirs 2 Turn on the gas supply and adjust the pressure to 55 KPa 8 psi Never pressurize the reservoirs above 69 KPa 10 psi Ne mettez jamais les r servoirs d luants sous une pression sup rieure 69 kPa 10 lb po 3 Operation and Maintenance Doc 031377 03 7 01 3 3 3 1 4 Start Up 1 Turn on the main pump switch The POWER UP screen displays briefly see Figure 2 3 and then the MAIN s
7. Doc 031377 03 7 01 C 5 C 1 3 Detail Screen The DETAIL screen provides the information contained in the MAIN screen see Section C 1 2 as well as the additional parameters described below Use the DETAIL screen to Display detailed information about the status of GP50 operating parameters Set parameters for Direct Control operation Figure C 4 Detail Screen TTL1 TTL2 RLY1 RLY2 Provides TTL and relay control of other devices In Direct control select 1 on or 0 off In Method control program the TTL and relay fields from the METHOD screen see Section C 1 4 Appendix D describes TTL and relay control CURVE Indicates the gradient curve selected in the current method step Refer to Section 2 9 4 for details about the curve types LIMIT Selects the low pressure and high pressure limits in psi MPa or bar See Section 3 1 5 for guidelines for selecting pressure limits Help Message CURVE 5 2 00 2125 10 25 MIN REMOTE COLUMN 05 DETAIL SCREEN 100 0 A TTL1 TTL2 RLY1 RLY2 0 0 1 1 INJECT 0 5000 PSI 0 0 B 0 0 C 0 0 D LIMIT METHOD PSI mL MIN SAMPLE A GP50 Gradient Pump C 6 Doc 031377 03 7 01 C 1 4 Method Screen Use the METHOD screen to select edit and save methods NOTE In the A B C D C V and FLOW fields a blank field indicates that the parameter for the previous step remains in effect Figure C 5 Method Screen METHOD EDIT
8. If Method 1 currently exists and you want to retain it enter a new unused method number in the EDIT field If Method 1 currently exists and you want to delete it move the cursor to TIME INIT and press Delete twice 2 Move the cursor to the A field and enter 60 move to the B field and enter 25 move to the C field and press Enter 15 is automatically filled in to make the eluent percentages equal 100 Ignore the C Curve and V Valve fields Move to the FLOW field and enter a flow rate of 2 00 Figure 3 6 Method Screen Isocratic Run Example Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 1 1 TIME A L 2 00 INIT 0 00 PSI B C D V gt gt gt gt 60 0 _ 25 0 15 0 GP50 Gradient Pump 3 14 Doc 031377 03 7 01 3 Move the cursor to SAVE TO and press Enter to save the method 4 Move the cursor to Run enter the method number 1 in this case and press Enter to select the programmed method If the pump motor is off press Off On to have the pump start delivering the eluent mixture 5 If the method clock is in hold press Hold Run to begin running the method 3 4 2 Linear Gradient Method Example The following summarizes the linear gradient method steps Create Method 2 to begin under isocratic conditions with 100 eluent A at 2 0 mL min After 5 minutes begin adding eluent B and decreasing eluent A until at 10 minutes the mixture is 65 eluent
9. Troubleshooting Doc 031377 03 7 01 4 3 4 2 Pump Will Not Start Flow rate is set to zero Reset the flow rate see Section 2 4 2 Pump starts briefly during priming but then stops because of high pressure limit Open the pressure transducer waste valve see Figure B 5 by turning the knob counterclockwise two turns 4 3 Pump Stops Method or other remote input instructed the pump to stop Check the display screen for error messages If none are displayed the pump was probably instructed to stop by the method computer or other remote signal source Electrical cables improperly installed 1 Set the pump to LOCAL mode DIRECT CONTROL Press Off On to start the pump 2 If a non zero flow rate is displayed and the keypad LED is on verify that the electrical cables in the mechanical chassis are properly installed a Turn off the GP50 power b Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door c Pull out the mechanical chassis drawer a few inches d Locate the distribution card on the top of the mechanical chassis Check that all cables are seated correctly in the connectors on the card e Push the mechanical chassis drawer back in place making sure the cables are not pinched Retighten the drawer lock Turn on the power GP50 Gr
10. A 3 A 7 Vacuum Degas Pump Assembly Optional A 3 B Installation B 1 Facility Requirements B 1 B 2 Installation Instructions B 2 B 2 1 Power Connection B 2 B 2 2 Electronics Chassis Connections B 4 B 2 3 DX LAN Interface 10BASE T Connections Optional B 6 Contents Doc 031377 03 7 01 v B 2 4 DX LAN Interface BNC Connections Optional B 9 B 2 5 Waste Lines B 13 B 2 6 Eluent Inlet Line Connections B 14 B 2 7 Eluent Outlet Line Connections B 14 B 2 8 Piston Seal Wash Connections Optional B 15 B 2 9 Priming the Pump B 17 B 3 Automatic SRS Power Control Optional B 20 C Display Screens C 1 Operational Screens C 2 C 1 1 Menu of Screens
11. 2 10 D 1 Contacting Dionex 4 1 Contamination 3 2 Contrast 2 2 Control modes 2 18 Direct 2 18 Flow C 12 Method 2 20 2 21 Pressure C 12 Selecting C 4 Specification A 3 Summary 2 19 Control panel 2 2 Keypad 2 3 Convex gradient curves 2 23 CPU card 2 10 Disposing of lithium battery 5 1 Troubleshooting 4 6 Creating a method 3 8 3 9 Examples 3 13 Cursor buttons 2 6 Cursor movement 2 6 Curve field Detail screen C 5 Curve types 2 23 Curved gradient example 3 19 Curves See Gradient curves D Daily maintenance 3 25 Degas Options screen C 9 Degas pump assembly 1 1 2 16 Calibration C 34 Calibration fails 4 10 Description 2 16 Flow schematic 2 13 Flushing 4 12 B 17 B 19 Low vacuum 4 11 Selecting operating cycles C 9 Specifications A 3 Testing 4 1 C 28 Troubleshooting 4 9 Degas Pump Calibration screen C 34 Degas Status screen C 28 Degassing eluents 2 17 3 1 B 1 Manual procedure 3 1 Troubleshooting 4 1 Delay volume A 2 Delete button 2 5 Deleting Field entries 2 5 Index Doc 031377 03 7 01 Index 3 Method steps 2 5 Methods 3 12 Description Calibration screens C 25 Diagnostic screens C 14 Functional 2 18 Physical 2 1 TTL input control D 3 User interface screens C 2 See also Physical description Detail status screen C 5 Diagnostic screens C 14 Accessing 2 6 Degas Status C 28 Diagnostic Test C 21 DSP Code Version C 24 DSP Status C 17 DX LAN
12. 2 Liter glass 2 L 2 Description Doc 031377 03 7 01 2 19 In Method control the GP50 executes commands according to the timed steps in a method The method is programmed from the GP50 front panel or from PeakNet 5 software See Section 2 9 3 for details about Method control from the front panel For information about using PeakNet 5 to program and run methods refer to the online Help or user s guide The table below summarizes the various operating and control mode configurations Select the modes from the MAIN screen see Section C 1 2 DETAIL screen see Section C 1 3 or chromatography software 2 9 2 Local and Remote Modes Local Mode When the GP50 power is turned on the pump is in Local mode In Local mode the pump accepts operating commands from two sources Direct input from the front panel keypad and screens With direct input all GP50 operating functions are available TTL inputs from a remote controller for example a Dionex detector module or autosampler TTL signals can be used to turn the pump motor on and off turn the method clock on and off and increment or decrement the method number Operating Control Mode Pump Operation Local Direct Control Commands are entered from the GP50 front control panel and executed immediately after being entered Local Method Commands are entered from the GP50 front control panel and executed by running a method programmed from the front panel Remote Dire
13. 3 23 Elapsed Time screen C 16 Electrical specifications A 1 Electronics cards See Printed circuit boards GP50 Gradient Pump Index 4 Doc 031377 03 7 01 Electronics chassis 2 8 Connections B 4 CPU 2 10 LC leak 2 9 B 4 LC30 communications B 5 TTL Relay 2 10 TTL Relay connections D 5 Eluent manifold 2 13 Flushing 3 27 Eluent reservoirs See Reservoirs Eluents Composition profile for gradient curves 2 22 Connecting lines to pump B 14 Degassing 2 16 3 1 4 1 Filtering 3 2 4 1 Flow schematic 2 13 3 26 Flushing lines after changing 4 12 Gradient delivery of 2 22 Isocratic delivery of 2 22 Method control of eluent mixture 2 20 Outlet line connections B 14 Pressuring reservoirs B 1 Preventing crystalization 3 25 Pump mixers 2 14 Specifying composition 3 6 3 7 C 3 C 7 Specifying flow rate C 3 C 7 Waste line B 13 End line filter 3 2 B 1 English On screen messages C 11 Enter button 2 6 Entry error sounds C 11 Environmental specifications A 1 Error messages Encoder index not found 4 6 High pressure limit violation 4 5 Language selection C 11 Low pressure limit violation 4 4 Motor drive fails 4 6 TTL2 is set to indicate FLOW NO FLOW 4 11 Vacuum degas fails 4 10 Example methods 3 13 3 24 External leak sensor C 27 F Facilities required B 1 Filter end line 3 1 3 2 B 1 Dirty or clogged 4 1 Filtered inert gas 3 1 3 2 Fittings Leaking 3 26 Flow Calibrat
14. C 2 C 1 2 Main Screen C 3 C 1 3 Detail Screen C 5 C 1 4 Method Screen C 6 C 1 5 Method Extension Screen C 8 C 1 6 Degas Options C 9 C 1 7 Module Setup C 11 C 1 8 Pump Options C 12 C 1 9 Time Function In C 13 C 2 Diagnostic Screens C 14 C 2 1 Diagnostic Menu C 14 C 2 2 Power Up Screen C 15 C 2 3 Elapsed Time C 16 C 2 4 DSP Status C 17 GP50 Gradient Pump vi Doc 031377 03 7 01 C 2 5 DX LAN Status C 18 C 2 6 Keyboard Test
15. Connect command 2 20 Selecting Locked Remote mode 2 20 PEEK liquid lines 5 2 Periodic maintenance 3 27 Physical description Control panel 2 3 Display screens 2 7 Mixers 2 14 Pressure transducer 2 15 Pump eluent manifold 2 13 Pump heads 2 14 Pump priming block 2 15 Rear panel 2 18 Vacuum degas pump assembly 2 16 Physical specifications A 1 Piston replacement 5 8 Piston seal replacement 5 4 Piston seal wash kit 2 16 Installation procedure B 15 Piston volume 2 14 Power requirements A 1 Power supply 2 10 Power switches 2 2 Power Up C 15 Power Up screen 2 7 3 3 Pressure Maximum operating 2 14 Pressure Calibration screen C 30 Pressure limits 3 4 C 6 Pressure mode C 12 Pressure transducer 2 15 Waste valve 2 15 B 18 Pressure units C 12 Pressurization points C 23 Pressurizing reservoirs B 1 Prime button 2 4 Priming B 17 Eluent manifold B 17 Problems during 4 3 Pump out of prime 4 1 4 2 With a syringe B 17 With alcohol B 19 With the Prime button B 18 Priming block 2 15 Priming valve Leaks from 4 7 Printed circuit boards 2 8 Connectors 2 9 CPU card 2 10 4 6 Distribution card 4 3 4 9 4 10 5 11 DSP card 2 9 4 5 4 6 DX LAN pump interface card 2 10 B 6 B 9 Host computer DX LAN card B 8 Relay I O card 2 10 Programming the pump 3 13 Gradient eluent run 2 22 Isocratic eluent run 2 22 3 13 Proportioning valve 2 13 2 17 Leaks 4 7 Malfunction 4 12 Replac
16. TTL and Relay Control Describes relay and TTL input and output functions and provides installation instructions 1 Introduction Doc 031377 03 7 01 1 3 1 2 1 Safety Messages and Notes This manual contains warnings and precautionary statements that when properly followed can prevent personal injury and or damage to the GP50 Safety messages appear in bold type and are accompanied by icons as shown below Informational messages also appear throughout this manual These are labeled NOTE and are in bold type NOTE NOTES call attention to certain information They alert you to an unexpected result of an action suggest how to optimize instrument performance etc Indicates an imminently hazardous situation which if not avoided will result in death or serious injury Indicates a potentially hazardous situation which if not avoided could result in death or serious injury Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury Indicates that the function or process of the instrument may be impaired Operation does not constitute a hazard GP50 Gradient Pump 1 4 Doc 031377 03 7 01 1 2 2 Safety Labels The TUV GS C US Mark safety label and the CE Mark label on the GP50 indicate that the GP50 is in compliance with the following standards EN 61010 1 1993 safety CAN CSA C22 2 No 1010 1 92 safety UL 3101 1 10 93 safety EN 50082 1 1992 susceptibilit
17. Use the DEGAS STATUS screen to check the status of the optional vacuum degas pump calibration parameters in real time and to test the degas pump Figure C 23 Degas Status Screen DEGAS READING The ADC reading from the degas pump pressure transducer STATUS Reports whether the degas pump is on or off ALARM THRESHOLD A degas reading value below this threshold value triggers the degas pump failure alarm TEST Select RUN and press Enter to test the degas pump The pump turns on and runs for 2 minutes While the pump is running the STATUS field indicates ON Note There may be a delay of several seconds before the on off status is updated Help Message DEGAS READING ALARM THRESHOLD DEGAS STATUS STATUS RDY OFF TEST nnnnn nnnnn C Display Screens Doc 031377 03 7 01 C 29 C 3 5 Flow Calibration Use the FLOW CALIBRATION screen to calibrate the pump Figure C 24 Flow Calibration Screen CALIBRATE To calibrate the flow select CAL and press Enter The pump will use standard calibration parameters for pump control When RDY is selected the pump uses stored parameters from the last calibration After selecting CAL follow the instructions on the screen for calibration The time required for calibration varies depending on the pump head volume 5 minutes for standard bore heads and 20 minutes for microbore heads PRESSURE Displays the measured value from the pressure transducer Select the pres
18. _ GP50 Gradient Pump 3 16 Doc 031377 03 7 01 3 Move the cursor to the A field of the TIME 0 step and press Enter 100 of eluent A is automatically filled in 4 Move the cursor to the line below TIME 0 and enter 5 to store a step at TIME 5 0 minutes Then move the cursor to A and press Enter to enter 100 and define a step with the same eluent composition as the previous step Although there is no change in eluent parameters the 100 of A must be entered at 5 00 minutes to establish 5 00 as the gradient start point This marks the end of the isocratic section of the run and the beginning of the eluent B concentration ramp From this point on the concentration of eluent A decreases from 100 as the concentration of eluent B begins to increase from 0 5 Move the cursor to the next line Enter 10 in the TIME field Move the cursor to the A field and enter 65 followed by 35 in the B field After a total of 10 minutes 5 minutes of isocratic conditions plus 5 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B the eluent composition is 65 eluent A and 35 eluent B This step marks the end of the second segment and the beginning of the eluent C concentration ramp Figure 3 9 illustrates the METHOD screen as it appears after Step 5 Figure 3 9 Linear Gradient Method Example After Step 5 Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE
19. 13 3 24 Gradient curve types 2 23 Gradient eluent run 2 22 INIT conditions 3 9 Isocratic eluent run 2 22 3 13 Linear gradient example 3 14 Number allowed 2 21 Running a method 3 10 Microbore heads 2 14 See also Pump heads MIN C 4 Mixers 2 14 mL MIN C 3 Modes of operation See Operating modes Module Setup screen C 11 Moduleware 2 10 MPa C 12 N Normal edge D 4 Normal pulse D 4 Numeric buttons 2 6 O Off On button 2 4 Offset calibration C 30 Operating configuration TTL D 3 Operating mode 2 18 Direct control 2 18 3 6 Local mode 2 18 2 19 3 6 3 7 Locked Remote mode 2 18 2 20 Method control 2 19 2 20 3 7 Remote mode 2 18 2 20 Selecting C 4 Selecting the modes 3 5 Operation 3 1 Aborting a running method 3 12 Creating a method 3 8 Curved gradient example 3 19 Deleting a method 3 12 Editing a method 3 11 Editing a running method 3 23 Gradient curve types 2 23 Gradient eluent run 2 22 INIT conditions 3 9 Isocratic eluent run 2 22 3 13 Linear gradient example 3 14 Method control 3 7 Preparing to run 3 1 Priming B 17 Programming the pump 3 13 Running a method 3 10 Running the pump 3 13 Selecting pressure limits 3 4 Shutdown 3 27 Operational screens 2 6 C 2 Outlet line connection B 14 P PeakNet 5 software 2 18 2 19 C 4 Selecting Locked Remote mode 2 20 GP50 Gradient Pump Index 8 Doc 031377 03 7 01 PeakNet 6 software 2 18 2 19 C 4
20. GND LOAD GND CBL PRESENT GND 5VDC lt 50mA GND AS40 Relay Control Cable P N 047946 PeakNet Chromatography Workstation DX LAN LOAD READY Pump Detector D TTL and Relay Control Doc 031377 03 7 01 D 7 Figures D 6 and D 7 show two different TTL relay connections for a Dionex system connected to an AS3500 Autosampler Setup 1 recommended for isocratic conditions starts running each Dionex module timed events method when the autosampler makes an injection Setup 2 recommended for gradient conditions starts running each Dionex module method before the autosampler makes an injection The method then controls when the autosampler injection occurs Refer to the AS3500 manual for details Figure D 6 Example TTL and Relay Connections AS3500 Setup 1 AS3500 RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN 1 2 3 4 5 PeakNet Chromatography Workstation DX LAN 6 7 8 9 10 11 12 Inject Output Ground Serial Comm Port Hold Run Hold Run Note The pump and detector must be set to NORMAL PULSE TTL input signal mode Pump Detector GP50 Gradient Pump D 8 Doc 031377 03 7 01 Figure D 7 Example TTL and Relay Connections AS3500 Setup 2 AS3500 Pump Detector RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 I
21. Keypad Main Power Requirements 100 Vac to 240 Vac 50 60 Hz 2 5 amps The GP50 power supply is main voltage auto sensing and requires no manual adjustment Fuse Requirements Two 3 15 amp fast blow IEC127 fuses P N 954745 Operating Temperature 10 C to 50 C 50 F to 104 F Operating Humidity 5 to 95 relative humidity non condensing Dimensions 33 5 cm high x 22 5 cm wide x 42 cm deep 13 1 in x 8 9 in x 16 8 in 6 cm 2 5 in clearance required behind the module Weight 19 kg 42 lb Decibel Level 60 db A WEIGHING setting Display Liquid crystal with adjustable backlighting Keypad 26 button pad for entering commands and numeric values for screen parameters GP50 Gradient Pump A 2 Doc 031377 03 7 01 A 5 Hydraulics Eluent Selection Four different eluent components each can be proportioned from 0 to 100 Pump Dual piston variable speed 100 L standard bore 25 L microbore stroke user selectable constant pressure or constant flow feedback control Flow Rate Standard bore pump head 0 04 to 10 0 mL min linearly variable in increments of 0 01 mL min Microbore pump head 0 01 to 2 50 mL min linearly variable in increments of 0 01 mL min Operating Pressure 35 MPa 5000 psi maximum Pressure Resolution 0 07 MPa 10 psi High Pressure Limit 0 to 35 MPa 0 to 5000 psi in increments of 0 05 MPa 7 25 psi trips instantaneously Low Pressure Limit 0 to 35 MPa
22. Off On to turn off the pump motor See Section B 2 8 for detailed priming instructions Inserts a new step into a method This button functions only when the cursor is in a TIME field on the METHOD or METHOD extension screen 1 Move the cursor to the TIME field and press Insert The new step is added below the cursor position Parameter values in the new step are blank 2 Fill in the time value and press Enter or a cursor arrow button Note If you move the cursor out of the TIME field before entering a time value the inserted step is not saved because it is incomplete 3 Insert steps in any order When you press Enter they will be automatically reorganized in the correct chronological order Table 2 1 GP50 Front Panel Buttons 2 Description Doc 031377 03 7 01 2 5 Removes the value from the current entry field To restore the previous value move the cursor from the field before entering a new value Pressing Delete when the cursor is in a step entry field on the METHOD screen blanks the step parameter value Moving the cursor to another field does not restore the previous value instead the step remains blank indicating no change from the previous step To delete an entire method step 1 Position the cursor in the method s time field and press Delete The time is removed and the help line prompts you to press Delete again to delete the step 2 Press Delete again Or to restore the original time an
23. VAC Chamber OUT OUT Proportioning Valve Manifold Assembly A B C D A B C D GP50 Gradient Pump 2 14 Doc 031377 03 7 01 2 4 2 Pump Heads There are two types of pump heads standard bore and microbore The table below summarizes the features and operating conditions for each type NOTE Although there is some overlap in flow rates between the two pump configurations continuous operation of the microbore pump at flow rates above 2 0 mL min will decrease seal and pump life For the best extended operation at 2 0 mL min or above use a standard bore pump To achieve optimum performance at flow rates below 0 4 mL min use a microbore pump 2 4 3 Pump Mixers A pump mixer ensures complete mixing of the proportioned eluents prior to injection The mixer is installed between the pump outlet and the injection valve Three types of mixers are available In standard bore PEEK pumps a GM 5 Mixer P N 054044 is used In microbore PEEK pumps a GM 4 Mixer P N 049136 is used In stainless steel pumps a Stainless Steel Mixer P N 054043 is used Pump Head Type Piston Volume Pump Operation Flow Rate mL min Maximum Operating Pressure Standard Bore 100 L Isocratic 0 04 10 0 35 MPa 5000 psi Gradient 0 4 10 0 35 MPa 5000 psi Microbore 25 L Isocratic and Gradient 0 0 2 50 35 MPa 5000 psi Flow rates are adjustable in increments of 0 01 mL min 2
24. VALUE OR PRESSURE CALIBRATION SELECT SLOPE CAL TO CONTINUE SLOPE DFLT SLOPE PRESSURE CAL MODE PSI OFFSET FLOW 1 00 m L MIN PSI 0 900 450 CAL VALUE 2500 PSI 0 GP50 Gradient Pump C 32 Doc 031377 03 7 01 Calibrating the Slope After selecting SLOPE CAL in Step 5 the screen displays the following 1 Close the waste valve 2 Install a pressure test gauge between the pressure transducer outlet and one of the following backpressure tubing or a separator column see Figure C 28 The pressure gauge assembly P N 046175 includes a gauge backpressure tubing and required fittings Figure C 27 Pressure Calibration Screen Third View Figure C 28 Pressure Gauge Installation Help Message CLOSE WASTE VALVE INSTALL GAUGE PRESSURE CALIBRATION TURN PUMP ON AND ENTER GAUGE PRESSURE SLOPE CAL SLOPE PRESSURE CAL MODE PSI OFFSET FLOW 1 00 m L MIN PSI 0 900 450 CAL VALUE 2500 PSI Pressure Transducer Outlet Backpressure Tubing Column Pressure Test Gauge or C Display Screens Doc 031377 03 7 01 C 33 3 In the FLOW field enter the flow rate normally used for your application 4 Turn on the pump The pressure will begin increasing Allow the pressure gauge reading to stabilize 5 Backpressure tubing If backpressure tubing is installed the recommended pressure to use for calibration is 17 25 0 17 MPa 2500 25 psi Add addit
25. a pas t con u cette fin GP50 Gradient Pump 2 18 Doc 031377 03 7 01 enclosure Each organizer can accommodate one or two reservoirs depending on the type of reservoir used see the table below 2 8 Rear Panel The rear panel contains the main power receptacle and fuses It also includes a DX LAN connector for interfacing the GP50 with the PeakNet workstation The rear panel is illustrated in Figure B 1 2 9 Functional Description 2 9 1 Operating and Control Modes The operating mode determines how the GP50 receives operating commands In Local mode the GP50 receives commands from the front control panel buttons and screens In Remote mode PeakNet 5 software sends commands from the host computer via the DX LAN interface Limited operating changes from the front panel are allowed In Locked Remote mode PeakNet 5 or PeakNet 6 software sends commands from the host computer via the DX LAN interface All operating changes from the front panel are disabled When PeakNet 6 software is controlling the GP50 the pump is always in Locked Remote mode The control mode determines when operating commands are executed In Direct control the GP50 executes commands immediately Because there is no time based program the method clock is not used and the Hold Run and Reset buttons do not operate No of Reservoirs Description Total Volume in Liters 2 1 Liter glass or plastic 2 L 2 2 Liter plastic 4 L 1
26. assembly c Reinstall the head and piston assembly using a wrench to tighten the nuts evenly 12 in lb torque 8 Reconnect the liquid line to the inlet check valve 9 Reconnect the tube fittings to the pressure transducer 10 Turn on the main power switch and prime the pump see Section B 2 8 GP50 Gradient Pump 5 10 Doc 031377 03 7 01 5 4 Pressure Transducer Waste Valve O Ring Replacement A damaged O ring causes leakage around the base of the pressure transducer waste valve knob 1 Turn off the main power switch to ensure you do not unintentionally start the GP50 2 Remove the valve from the pressure transducer housing by turning the knob counterclockwise until it comes loose from the housing 3 Remove the O ring P N 046434 see Figure 5 6 4 Carefully slide a new O ring P N 046434 over the end of the valve and push it into the groove 5 Reinstall the valve in the housing turning the knob clockwise until the valve is seated Do not overtighten the waste valve Figure 5 6 Pressure Transducer Assembly Needle Valve P N 042625 Screw 4 P N 053988 Gasket P N 054417 Gasket Support P N 054065 Transducer Housing P N 054052 O Ring P N 046434 Transducer Sleeve P N 054053 Transducer Cable Assembly P N 054064 Washer 4 P N 045689 5 Service Doc 031377 03 7 01 5 11 5 5 Proportioning Valve Replacement A defective proportioning valv
27. cursor to the C field and enter 3 to run gradient curve 3 After a total of 3 5 minutes 1 minute of isocratic conditions plus 2 5 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B the eluent composition is 68 eluent A and 32 eluent B Figure 3 15 illustrates the METHOD screen as it appears after Step 6 Figure 3 14 Curved Gradient Method Example After Step 2 Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 3 3 TIME A L 1 50 INIT 0 00 PSI B C D V gt gt gt gt 100 0 _ GP50 Gradient Pump 3 22 Doc 031377 03 7 01 7 Move the cursor to the next line and enter 6 in the TIME field Move the cursor to B and enter 100 8 Move the cursor to the C field and enter 8 After a total of 6 minutes the eluent composition is 0 eluent A and 100 eluent B 9 Move the cursor to the next line and enter 7 in the TIME field Move the cursor to B and enter 100 The eluent composition remains unchanged at 100 eluent B for 1 minute 10 Move the cursor to the next line and enter 10 in the TIME field Move the cursor to A and enter 100 After a total of 10 minutes the concentration of eluent B drops to zero and the concentration of eluent A increases to 100 Figure 3 16 illustrates the completed METHOD screen 11 Move the cursor to the SAVE TO field and press Enter to save the method to memory Figure 3 15
28. entry field If there is no changeable field in that direction the cursor moves diagonally or remains in its current location In most cases after entering a new value in an entry field pressing an arrow button saves and or executes the change The exceptions are the METHOD SAVE TO field the METHOD RUN field any calibration command the DIAGNOSTIC TESTS screen and all menu screens Displays a help screen with information pertaining to the current entry field Displays one of three menus depending on the current screen From an operational screen pressing Menu displays the MENU of SCREENS From a diagnostic screen pressing Menu displays the DIAGNOSTIC MENU From a calibration screen pressing Menu displays the CALIBRATION MENU Enters numeric values into the current entry field The numeric buttons are 0 through 9 and the decimal From a menu screen pressing a numeric button opens the corresponding screen Saves and or executes changes made in entry fields If a menu screen is displayed pressing Enter opens the highlighed screen Keypad Buttons Function Table 2 1 GP50 Front Panel Buttons Continued 2 Description Doc 031377 03 7 01 2 7 2 1 2 Display Screens When the pump has successfully powered up and passed all diagnostic tests the POWER UP screen see Figure 2 3 displays briefly followed by the MAIN screen see Figure 2 4 If one of the diagnostic tests fails at power up the DIAGNOSTIC TEST sc
29. field displays LOCAL REMOTE or LOCKED RMT The control mode field displays DIRECT CNTRL or METHOD See the example in Figure 3 2 2 To select the operating mode If the GP50 is controlled by PeakNet 6 connecting to the PeakNet 6 timebase automatically selects LOCKED RMT To return the GP50 to LOCAL either clear the Connect check box on the PeakNet 6 control panel or turn the GP50 power off and then on If the GP50 is controlled by PeakNet 5 select LOCAL or REMOTE on the MAIN or DETAIL screen Move the cursor to the field press Select or Select to toggle to the desired mode and press Enter or a cursor arrow button To select LOCKED RMT with PeakNet 5 select the Locked Remote Start option from the Run program To return the GP50 to LOCAL clear the Start option or turn the GP50 power off and then on 3 To select the control mode move the cursor to the field press Select or Select to toggle to the desired mode and press Enter or a cursor arrow button Figure 3 2 Main Screen Operating and Control Mode Fields Control Mode Operating Mode Help Message PSI LOAD DIRECT CNTRL 60 0 A 25 0 B 15 0 C 0 0 D COLUMN A 2000 mL MIN 2 00 LOCAL GP50 Gradient Pump 3 6 Doc 031377 03 7 01 3 2 Running Under Direct Control Local Mode Direct Control Local mode is used most often when a PeakNet workstation is not configured Direct Control Example Specify an isocratic mixture of 6
30. gt 15 00 25 00 45 0 55 0 100 0 20 00 40 0 60 0 gt GP50 Gradient Pump 3 24 Doc 031377 03 7 01 This example assumes that the example Method 2 is currently running 1 Go to the METHOD screen and enter 2 in the EDIT field 2 Move the cursor down through Method 2 until you reach the TIME 15 step Move the cursor to B and enter 45 Move the cursor to C and enter 55 3 Move the cursor to the TIME field and press Insert Enter 20 in the time field Move the cursor to B and enter 40 Move the cursor to C and enter 60 4 Move the cursor to the SAVE TO field and press Enter 5 Press Menu and select either the MAIN screen or DETAIL screen Check the status of the method clock If the elapsed time is less than 15 minutes the time for the first change made to the method the changes will be incorporated into this run and executed at the programmed time If the elapsed time is greater than 15 minutes the changes will not be incorporated into this run To put the changes into effect either press Reset to set the method clock to the INITial conditions or enter an elapsed time in the MIN field that is less than 15 10 for example The method will restart using the parameters programmed for TIME 10 and the method changes will be incorporated at the programmed time 3 Operation and Maintenance Doc 031377 03 7 01 3 25 3 5 Routine Maintenance This section describes routine maintenance
31. in a method Each step specifies the eluent composition and flow rate to be delivered by the pump at a given time The selected eluent mixture is delivered either isocratically or as a multistep linear or curved gradient As the method runs the GP50 calculates the changes in eluent composition required to deliver a gradient from one method step to the next or to match the selected curve 2 Description Doc 031377 03 7 01 2 21 Methods are programmed saved and edited from the METHOD screen see Figure 2 9 See Section 3 3 for programming instructions NOTE For information about using PeakNet 5 to program and run methods refer to the online Help or user s guide Here is a summary of basic information about using methods Each method can contain up to 50 time based steps Step 1 always starts at INIT initial condition Step 2 always starts at TIME 0 0 The GP50 can store up to 100 separate methods 0 through 99 in memory Methods are retained in memory even after the pump is powered down The pump can run under method control while you are entering or editing any method When you save changes to the currently running method or switch to a different method the method clock continues running unaffected Only those parameter changes which affect the method after the current time will be implemented in the current run Figure 2 9 Method Screen The total number of methods that can be stored in memory depends on the
32. optional vacuum degas pump is available for all versions GP50 Gradient Pump Version With Degas Pump Without Degas Pump Standard bore with PEEK components P N 054426 P N 054427 Standard bore with stainless steel components P N 054419 P N 054420 Microbore with PEEK components P N 054045 P N 054429 Microbore with stainless steel components P N 054425 P N 054424 GP50 Gradient Pump 1 2 Doc 031377 03 7 01 1 2 About This Manual Chapter 1 Introduction Gives an overview of the GP50 Gradient Pump and explains conventions used in this manual including safety related information Chapter 2 Description Describes the GP50 front panel controls electronic and mechanical components and operating modes Chapter 3 Operation and Maintenance Provides an overview of GP50 operation including how to create edit and run methods from the GP50 front panel Lists routine preventive maintenance procedures Chapter 4 Troubleshooting Lists problems and presents step by step procedures for how to isolate and eliminate them Chapter 5 Service Provides step by step instructions for routine service and parts replacement procedures Appendix A Specifications Lists the GP50 specifications and installation site requirements Appendix B Installation Describes how to install the GP50 Appendix C Display Screens Illustrates and describes all of the screens that can be displayed on the GP50 front panel Appendix D
33. printed on the card identify the various cables plugged into it Locate the connector for the vacuum degas pump labeled VAC PUMP near the right rear corner of the card Make sure the cable is fully seated in the connector 4 Push the mechanical chassis drawer back in place making sure the cables are not pinched Retighten the drawer lock 5 Turn on the power 6 Retry the calibration If the message reappears contact Dionex Technical Support Degas vacuum pump is not present or degas circuitry is malfunctioning Vacuum Degas Fails 4 Troubleshooting Doc 031377 03 7 01 4 11 4 8 Vacuum Degas Pump Low Vacuum The GP50 monitors the degas vacuum reading every 1 min If the degas vacuum is lower than the monitoring value the degas pump turns on When the pump turns off if the vacuum reading is 2000 counts or more lower than the monitoring value the following message displays Open the DEGAS OPTIONS screen press Menu and 4 Try increasing the CYCLE DURATION time and or decreasing the TIME BETWEEN CYCLES If this does not resolve the problem contact Dionex Technical Support 4 9 Relays or TTLs Inoperative Incorrectly installed cables Make sure the cable s between the GP50 relay or TTL connector s and the connector s on the other instrument s are connected properly see Section D 3 Method programming error For Local control make sure the parameter to turn on or off the relay TTL is set correctly on
34. the METHOD EXTENSION screen see Section C 1 5 For Remote control make sure the command for turning the relay TTL on or off is entered correctly into the PeakNet 6 program file or the PeakNet 5 Method For TTL inputs make sure the controlling device is programmed correctly When attempting to set TTL2 the following message displays The TTL2 OUTPUT USAGE field on the PUMP OPTIONS screen is currently set to signal when pump flow stops 0 FLOW This setting is used to control the power to a Self Regenerating Suppressor SRS To use TTL2 for another function open the PUMP OPTIONS screen press Menu and 6 and set the TTL2 OUTPUT USAGE field to NORMAL LOW VACUUM ALARM Check DEGAS OPTIONS settings or refer to service manual TTL2 is set to indicate FLOW NO FLOW GP50 Gradient Pump 4 12 Doc 031377 03 7 01 4 10 Poor Chromatographic Reproducibility Liquid lines incompletely flushed after an eluent change Attach a syringe to the priming block valve see Figure B 5 and draw at least 2 5 mL 20 mL if the vacuum degas pump is installed of the new eluent through the liquid lines before beginning operation Leaking piston seal Check for liquid leaks at the rinse ports in the front of the pump heads Replace the piston seal on any head with a leak see Section 5 2 Malfunctioning proportioning valve Test the valves see Section C 2 7 If a test fails the proportioning valve assembly may need to be replaced see Sect
35. the bottle above the GP50 During operation gravity maintains flow through the pump heads 2 Cut the clear 1 5 mm 0 06 in ID tubing P N 055847 into three unequal pieces of the following lengths 115 cm 46 in 150 cm 60 in 15 cm 6 in 3 Install barbed fittings P N 037431 on the pump heads as shown in Figure B 4 and push the pieces of clear 1 5 mm 0 06 in ID tubing onto the barbed fittings 4 Place the free end of the 115 cm 46 in piece of tubing into the wash bottle Make sure the end of the tubing is near the bottom of the bottle 5 Slide a 10 mL syringe P N 054578 over the free end of the 150 cm 60 in piece of tubing the waste line Draw out all of the air to start the liquid flow through the pump heads Remove the syringe 6 Push the piece of black 0 25 mm 0 01 in ID PEEK tubing P N 057057 into the waste line until the PEEK tubing is inserted about 5 cm 2 in into the waste line tubing 7 Place the waste line in a waste container NOTE Assuming a height difference of about 30 inches between the wash bottle and the waste container the setup described here provides a flow of one drop of liquid every 10 to 12 seconds GP50 Gradient Pump B 16 Doc 031377 03 7 01 Figure B 4 Piston Seal Wash Connections Silicone Tubing P N 055847 115 cm 46 in Silicone Tubing P N 055847 15 cm 6 in Silicone Tubing P N 055847 150 cm 60 in into the silicone tubing about 5
36. the door Tab for tilting the panel Knob for adjusting the contrast Main Power Switch Actuator Help Message PSI LOAD METHOD 100 0 A 0 0 B 0 0 C 0 0 D COLUMN A 2000 mL MIN 2 00 LOCAL 2 MIN 10 27 GP50 Gradient Pump 2 4 Doc 031377 03 7 01 enter variable values or use the Select and Select buttons to choose from among predetermined options To confirm the selected value press Enter or an arrow button In some screens or screen fields pressing Enter is required NOTE A high pitched beep sounds when you press a button When an error occurs this beep is lower in frequency The beeps can be disabled from the MODULE SET UP screen see Section C 1 7 Keypad Buttons Function Turns the pump motor off and on In Direct control see Section 2 9 turning on the motor causes it to pump isocratically using the displayed eluent percentages and flow rate In Method control see Section 2 9 3 turning on the motor causes it to pump at the eluent percentages and flow rate for the elapsed time of the selected method or at the initial conditions when the method clock is at INIT This button is used when priming the pump heads Prime causes the pump to run at maximum volume 2 5 mL min microbore 10 0 mL min standard bore If the pump motor is off when Prime is pressed the pump automatically turns on To exit priming and return to the normal flow rate press Prime again or press
37. the parameters specified in the step for the current elapsed time To start the method at the INITial conditions press Reset 3 3 6 Controlling the Method Clock The Hold Run button the Reset button and the MIN fields in the MAIN and DETAIL screens control the method clock To start and stop the method clock press Hold Run To reset the clock to INITial conditions press Reset To set the clock to a specific elapsed time enter the time in the MIN field on the MAIN or DETAIL screen The method will start or continue running using the method parameters specified for that time 3 Operation and Maintenance Doc 031377 03 7 01 3 13 3 4 Example Methods The examples in this section provide step by step instructions for creating three types of methods isocratic linear gradient and curved gradient The last example demonstrates how to edit a running method For all of the examples set the pump to LOCAL mode METHOD control see Section 3 1 6 3 4 1 Isocratic Method Example Specify an isocratic mixture of 60 eluent A 25 eluent B and 15 eluent C to be pumped at 2 0 mL min Figure 3 3 illustrates the isocratic profile for this example Figure 3 6 illustrates the METHOD screen as it appears when the example is set up 1 Go to the METHOD screen and enter a method number in the EDIT field 1 for example The screen automatically changes the number in the SAVE TO field to the number of the method being edited
38. the tee connector to verify that it cannot move 2 Install the coaxial DX LAN cable P N 960405 a Push the metal sleeve on the end of the DX LAN cable onto one port of the BNC tee connector b Twist the metal sleeve until the locking pins on the tee are fully engaged in the slots on the cable s metal sleeve c Pull the end of the cable to verify that it cannot move 3 If the pump is the last module in the network to be connected install a 50 ohm terminator resistor plug P N 921034 on the remaining port of the BNC tee connector These terminator resistor plugs are shipped with Dionex chromatography software If the pump is not the last module in the network connect the cable from the next module to the BNC tee The DX LAN cable P N 960405 is a 50 ohm coaxial impedance cable Fifty ohm cables are imprinted with RG 58U Do not substitute cables Failure to use the correct cable or to lock it into place on the BNC connector will cause the pump to lose communication with the host computer GP50 Gradient Pump B 12 Doc 031377 03 7 01 4 Plug the free end of the coaxial DX LAN cable into one of these The BNC connector on another instrument or The BNC port on the rear panel of the combo 10BASE T Ethernet hub P N 056909 If the connection terminates at the hub install a BNC terminator included with the hub on the BNC tee connector A 50 ohm terminator resistor plug must be installed at ea
39. there are additional steps below Move the cursor to the bottom time entry and press the down arrow to see the additional step s The symbol next to the top time entry indicates that it is preceded by at least one more step Move the cursor to the entry and press the up arrow to see the additional step s The symbol gt at the right edge of each line indicates a lateral extension to the line Move the cursor to the end of a line and press the right arrow to display the METHOD extension screen see Section C 1 5 3 3 1 Creating a New Method You can create a new method when the method clock is in either Hold or Run 1 Go to the MAIN or DETAIL screen and if necessary change the operating and control modes to LOCAL and METHOD see Section 3 1 6 2 Go to the METHOD screen 3 In the EDIT field enter the number of the method to be created This can be the number of an unused method or of an existing method that you plan to edit and save as a new method If you enter the number of an unused method the screen will look similar to the example screen in Figure 3 5 3 Operation and Maintenance Doc 031377 03 7 01 3 9 4 In the LIMITs field set the low and high pressure limits see Section 3 1 5 5 Each method starts out with two timed steps see Figure 3 5 an initial conditions step containing INIT in the TIME column and a time zero step containing 0 00 in the TIME column The parameters in each of these
40. to the LC COMM connector in slot 1 When connected the LC30 can be remotely controlled by the PeakNet workstation Figure 2 6 GP50 Electronics Chassis Located behind pump door POWER SUPPLY GREEN OK RED FAULT SLOT 3 BLANK I LAN 000K SLOT 4 BLANK SLOT 2 SLOT 1 GP50 IP25 DSP 130W PWR SPY 1 I I P7 D S T 3 P N 1 I I P8 D S T 2 P N MOTOR L C A R L C C O M M L C L E A K F R O N T P A N E L RLY 1 OUT OUT RLY 2 TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN P6 CPU RLY SLOT 5 DIST GP50 Gradient Pump 2 10 Doc 031377 03 7 01 LC AIR The cable from the air solenoid valves in the LC10 LC20 LC25 or LC30 chromatography module connects to the LC AIR connector in slot 1 When connected the GP50 can electrically actuate the solenoid valves that control the position of the injection valve and the optional column switching valve in the chromatography module To select the valve positions go to either the MAIN screen see Section C 1 2 or the METHOD screen see Section C 1 4 TTL RELAY A strip of eight relay and TTL connectors is located in slot 4 These connectors interface with Dionex and non Dionex modules for relay and TTL control of the pump Appendix D describes the relay and TTL functions and the connect
41. to wait between cycles 1 99 min Help Message DEGAS PUMP DEGAS OPTIONS START UP DURATION 2 TIME BETWEEN CYCLES MIN 10 BY SETTING CYCLE DURATION SEC MIN 30 DEFAULT 2 MIN 10 SEC MIN 30 GP50 Gradient Pump C 10 Doc 031377 03 7 01 The GP50 monitors the degas vacuum reading every one minute If the degas vacuum reading is at or below the alarm threshold value 2000 counts below the calibration threshold value the following message displays If this occurs increase the CYCLE DURATION time and or decrease the TIME BETWEEN CYCLES If adjusting these settings does not solve the problem contact Dionex Technical Support LOW VACUUM ALARM Check DEGAS OPTIONS settings or refer to service manual C Display Screens Doc 031377 03 7 01 C 11 C 1 7 Module Setup Use this screen to adjust the display backlight and to toggle the key actuation and error tones on or off Adjustments made in this screen will be the default until new adjustments are made NOTE If no keypad buttons are pressed within a two hour period the backlight will automatically turn off Press any button to restore the backlight Figure C 8 Module Setup Screen DISPLAY PANEL BACKLIGHT Sets the backlight to LOW MEDIUM HIGH or OFF KEY ACTUATION SOUND Toggles the key actuation sound ON or OFF ENTRY ERROR SOUND Toggles the error entry sound ON or OFF HELP LANGUAGE Selects the language English or
42. until it is flush with the head Figure 5 5 Installing the Piston Seal View A Seal Piston Guide Piston Push the piston through the seal and guide and partially insert into the head just until the seal contacts the counterbore Counterbore Pump Head View B Remove the piston and push the piston guide into the head to finish seating the seal GP50 Gradient Pump 5 8 Doc 031377 03 7 01 9 Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself To do this a Press the backup washer into the head until it is flush with the indented surface of the head b Insert the piston halfway into the head This ensures that the magnet in the follower picks up the piston The follower is the cylinder that holds the piston in place as it moves in and out of the pump head assembly c Reinstall the head and piston assembly using a wrench to tighten the nuts evenly 12 in lb torque 10 Reconnect the liquid line to the inlet check valve 11 Reconnect the tube fittings to the pressure transducer 12 Turn on the GP50 power 13 Go to the ELAPSED TIME screen press Menu 8 and 2 Press Enter to reset the SEALS IN USE field to 0 cycles The pump is ready for normal operation 5 3 Pump Piston Replacement Continued leaking through the rinse ports after replacing the piston seal assuming the head is tight indicates a dirty scratched or broken pisto
43. 0 eluent A 25 eluent B and 15 eluent C to be pumped at 2 0 mL min see Figure 3 3 1 Go to the MAIN or DETAIL screen and if necessary change the operating and control modes to LOCAL and DIRECT CNTRL see Section 3 1 6 Figure 3 4 illustrates the MAIN screen as it will appear when the example is set up Figure 3 3 Isocratic Run Profile Figure 3 4 Main Screen Running Under Direct Control Help Message PSI LOAD DIRECT CNTRL 60 0 A 25 0 B 15 0 C 0 0 D COLUMN A 2000 mL MIN 2 00 LOCAL 3 Operation and Maintenance Doc 031377 03 7 01 3 7 2 Move to the A field and enter 60 move to the B field and enter 25 move to the C field and press Enter 15 is automatically filled in to make the eluent percentages equal 100 3 Move to the mL MIN field and enter 2 4 If the pump is currently off press Off On to turn on the motor and begin the isocratic delivery NOTE After starting the pump or changing the flow rate wait at least 5 minutes up to 20 minutes for low flow rates in a standard bore pump before beginning an analysis This allows the flow rate to stabilize 3 3 Running Under Method Control Local Mode This section provides general instructions on how to create edit and run methods from the GP50 front panel For step by step examples see Section 3 4 When entering parameters on the METHOD screen observe these guidelines After starting the pump or changing the flow rat
44. 0 to 5000 psi in increments of 0 05 MPa 7 25 psi trips after a time out of 0 4 mL for the standard bore GP50 or 0 1 mL for the microbore GP50 Delay Volume No Mixer Standard bore pump head lt 700 L Microbore pump head lt 300 L A Specifications Doc 031377 03 7 01 A 3 A 6 Control A 7 Vacuum Degas Pump Assembly Optional Remote Limited remote operation via TTL input logic level and TTL output and relay contact closures or full remote programming and control via the DX LAN interface Local Front Panel Methods Stores up to 100 separate methods 00 through 99 The actual number of stored methods depends on available memory Each method may contain up to 50 separate steps Storage Nonvolatile memory protects against the loss of programs during power down or in the event of a power failure Channels 4 channel membrane vacuum degas Materials Wetted materials PEEK PTFE GP50 Gradient Pump A 4 Doc 031377 03 7 01 Doc 031377 03 7 01 B 1 B Installation B 1 Facility Requirements Make sure the GP50 installation site meets the electrical and environmental requirements in Appendix A Install the GP50 on a sturdy workbench at a height that ensures convenient viewing of the front panel display and access to the interior Allow at least 6 cm 2 5 in free space behind the GP50 for connections and ventilation The GP50 Gradient Pump is capable of operation with or without head pressur
45. 1 3 19 3 4 3 Curved Gradient Method Example When attempting to optimize the separation of a single component in a complex mixture it is often helpful to employ paired segments of a curved gradient For example if the analyte is an oligonucleotide that elutes with 0 32 M NaCl in a linear gradient run you can move potentially interfering components of the sample away from the target oligonucleotide by first programming a segment from 0 to 32 of a 1 M NaCl solution using curve 2 Then program a segment from 32 to 100 of 1 M NaCl using curve 8 The resulting gradient profile is shown in Figure 3 12 In binary curve gradients two elements follow curves which are mirror images e g E1 Curve 8 and E2 Curve 1 in Figure 3 13 See Section 2 9 4 for details about gradient curves Figure 3 12 Gradient Curve Profile GP50 Gradient Pump 3 20 Doc 031377 03 7 01 The following summarizes the curved gradient method example steps Create Method 3 to begin under isocratic conditions with 100 eluent A 25 mM Tris buffer pH 8 0 at 1 5 mL min After 1 minute begin a convex addition of eluent B 25 mM Tris buffer pH 8 0 containing 1 0 M NaCl while decreasing eluent A until after 2 5 minutes elapsed time 3 5 minutes the mixture is 68 eluent A and 32 eluent B Begin a 2 5 minute concave segment in which the amount of eluent A is decreased and the amount of eluent B is increased until the mixture is 100 eluent B elapsed
46. 6 7 8 9 10 2 Description Doc 031377 03 7 01 2 23 Curve numbers are defined as follows NOTE A curve number in a step instructs the pump to use the selected curve number when moving from the previous step to that step Because there are no previous steps for INIT or TIME 0 0 curve numbers cannot be entered for these steps The gradient slope does not change during a step if curve 5 the default setting is selected because curve 5 represents a linear gradient Convex curves cause rapid changes in eluent composition at the beginning of the curve and slower changes at the end Concave curves cause slower changes at the beginning and rapid changes at the end Slope changes over time become more extreme as curves go from 6 to 9 more concave and from 4 to 1 more convex Figure 2 10 shows the eluent composition profiles corresponding to curves 1 through 9 normalized for 0 100 for 10 minutes Any consecutive method steps specifying identical eluent compositions will generate an isocratic segment regardless of the curve number selected A step change is a steep linear gradient in which the eluent composition changes from one eluent to another within 6 seconds Curve Number Gradient Type 1 2 3 4 Convex 5 Linear power up default 6 7 8 9 Concave GP50 Gradient Pump 2 24 Doc 031377 03 7 01 Doc 031377 03 7 01 3 1 3 Operation and Maintenance 3 1 Getting Ready to Run NOTE The GP50
47. A and 35 eluent B Begin adding eluent C to the mixture while continuing to decrease eluent A and increase eluent B until at 15 minutes the eluent composition is 0 eluent A 50 eluent B and 50 eluent C Continue increasing eluent C and begin decreasing eluent B until at 25 minutes the eluent composition is 100 eluent C Make a step change to 100 eluent D at 25 01 minutes Pump 100 eluent D for 4 99 minutes At 30 01 minutes return to 100 eluent A and re equilibrate your system for the next analysis Figure 3 7 illustrates the gradient profile for this method 3 Operation and Maintenance Doc 031377 03 7 01 3 15 1 Go to the METHOD screen and enter a method number in the EDIT field 2 for example If Method 2 currently exists and you want to retain it enter a new unused method number in the EDIT field If Method 2 currently exists and you want to delete it move the cursor to TIME INIT and press Delete twice 2 Move to the FLOW field of the INIT step and enter a flow rate of 2 00 Figure 3 8 illustrates the METHOD screen as it appears so far You can now begin entering the method steps that will generate the gradient profile Figure 3 7 Linear Gradient Method Profile Figure 3 8 Linear Gradient Method Example After Step 2 Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 2 2 TIME A L 2 00 INIT 0 00 PSI B C D V gt gt gt gt 100 0
48. ATUS DX LAN STATUS DIAGNOSTIC TEST PRESSURE STATISTICS CALIBRATION MENU DSP CODE VERSION 1 2 3 4 5 6 7 8 DIAGNOSTIC MENU KEYBOARD TEST 9 C Display Screens Doc 031377 03 7 01 C 15 C 2 2 Power Up Screen Use the POWER UP screen to check the volume of the pump head installed the revision levels of the Moduleware and BIOS installed and the ID number of the DX LAN interface if connected The POWER UP screen is automatically displayed when the GP50 power is turned on Figure C 12 Power Up Screen Help Message GP50 GRADIENT PUMP PUMP HEAD VOLUME 100 uL MODULEWARE REV n nn BIOS REV n nn GP50 Gradient Pump C 16 Doc 031377 03 7 01 C 2 3 Elapsed Time Use the ELAPSED TIME screen to check for how long various GP50 components have been in use The status of each parameter updates in real time Figure C 13 Elapsed Time Screen MODULE ON Reports the total time the module has been powered up in its lifetime BACKLIGHT Reports the total time the display backlight has been on in its lifetime PUMP CYCLES Reports the total cumulative number of pump cycles during the life of the motor This field automatically resets to 0 after the pump motor is replaced SEALS IN USE Reports the total number of pump cycles since the last time the seals were replaced Reset this field to 0 when the seals are replaced VALVES IN USE Reports the total number of pump cycles since the last time the chec
49. Curved Gradient Method Example After Step 6 Figure 3 16 Curved Gradient Method Example Complete Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 3 3 TIME A L 1 50 INIT 0 00 PSI B C D V gt gt gt gt 100 0 _ 100 0 1 00 100 0 3 50 68 0 32 0 3 Help Message C FLOW METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 3 3 TIME A 1 50 PSI B C D V gt gt gt gt 3 50 68 0 32 0 3 6 00 100 0 8 7 00 100 0 10 00 100 0 gt 3 Operation and Maintenance Doc 031377 03 7 01 3 23 3 4 4 Editing a Running Method Example After entering a method you can modify it by changing adding or deleting steps The example describes how to make the following changes to Method 2 the linear gradient example see Section 3 4 2 Change the eluent composition at TIME 15 from 50 eluent B and 50 eluent C to 45 eluent B and 55 eluent C Add a step to Method 2 at TIME 20 0 to make the eluent composition 40 eluent B and 60 eluent C Figure 3 17 illustrates the METHOD screen as it will appear when editing is complete Figure 3 18 illustrates the edited gradient profile Figure 3 17 Edited Linear Gradient Method Example Figure 3 18 Gradient Profile After Editing Help Message C FLOW METHOD EDIT LIMITs 0 5000 SAVE TO RUN 2 2 2 TIME A 2 00 PSI B C D V gt gt gt gt 10 00 65 0 35 0
50. Description Doc 031377 03 7 01 2 15 2 4 4 Pump Priming Block The priming block tee directs the flow of eluent from the manifold into the pump heads The priming block is also used for rapid removal of air from the system Refer to Section B 2 8 for instructions on priming the pump heads 2 4 5 Pressure Transducer From the priming block the liquid stream is directed to the inlet check valves on the pump heads through the pump heads and finally through the outlet check valves to the pressure transducer Flow from the outlet check valves on the pump heads is combined in the pressure transducer The pressure transducer measures the system pressure at this point The interactive constant flow constant pressure control program on the DSP precisely controls the pump motor speed to assure flow rate accuracy A waste line exits the bottom of the pressure transducer Opening the valve on the pressure transducer diverts flow to the waste line and relieves system pressure forcing air out of the system Flow output from the pressure transducer is directed out of the pump module through the gradient mixer and on to the rest of the chromatography system injection valve column detector See Section B 2 6 for eluent outlet line connections GP50 Gradient Pump 2 16 Doc 031377 03 7 01 2 5 Vacuum Degas Pump Assembly Optional The Dionex vacuum degas pump provides continuous on line vacuum degassing of up to four eluen
51. Display Screens Doc 031377 03 7 01 C 13 C 1 9 Time Function In Use the TIME FUNCTION IN screen to Select a TTL input signal mode Display the pump functions that can be controlled via TTL input from another device See Appendix D for details about TTL controlled functions and connections Figure C 10 Pump Options Screen MODE Select the signal mode NORMAL EDGE INVERTED EDGE NORMAL PULSE or INVERTED PULSE that corresponds to the signal type of the controlling device The default mode normal edge is compatible with the TTL output signals provided by Dionex modules Help Message HOLD RESET INIT COND RUN INCREMENT METHOD NUMBER 1 2 3 4 TIME FUNCTION IN PUMP MOTOR OFF ON MODE NORMAL EDGE TTL TTL TTL TTL DECREMENT METHOD NUMBER GP50 Gradient Pump C 14 Doc 031377 03 7 01 C 2 Diagnostic Screens C 2 1 Diagnostic Menu The DIAGNOSTIC MENU lists the available diagnostic screens To go to the menu select option 8 from the MENU of SCREENS There are two ways to select a screen from the menu Press the numeric button on the GP50 front panel keypad that corresponds to the screen number on the menu For example press 8 to select and display the CALIBRATION MENU Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Figure C 11 Diagnostic Menu Help Message POWER UP SCREEN ELAPSED TIME DSP ST
52. E VERSION DSP BIOS REV DSP PRESSURE MODE REVnn nn DSP CAL REV n nn nn nn DSP FLOW MODE REV active nn nn C Display Screens Doc 031377 03 7 01 C 25 C 3 Calibration Screens C 3 1 Calibration Menu To go to the CALIBRATION MENU select option 8 from the DIAGNOSTIC MENU There are two ways to select a screen from the menu Press the numeric button on the GP50 front panel keypad that corresponds to the screen number on the menu For example press 4 to select and display the FLOW CALIBRATION screen Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Figure C 20 Calibration Menu Screen Help Message CALIBRATION STATUS LEAK CAL amp STATUS DEGAS STATUS PRESSURE CALIBRATION DEGAS PUMP CALIBRATION 1 2 3 4 5 6 CALIBRATION MENU FLOW CALIBRATION GP50 Gradient Pump C 26 Doc 031377 03 7 01 C 3 2 Calibration Status Use the CALIBRATION STATUS screen to check the status of calibration parameters in real time Figure C 21 Calibration Status Screen FLOW RATE CAL The current flow rate calibration value PRESSURE OFFSET The pressure offset calibration value as reported by the DSP digital signal processor C E CAL VALUE The current binary value for cam encoder calibration as reported by the DSP Help Message FLOW RATE CAL PRESSURE OFFSET n nn g CALIBRATION STATUS C E CAL VALUE n
53. EGAS CALIBRATION VALUE Reports the calibration value recorded during the last calibration Help Message CALIBRATE DEGAS THRESHOLD DEGAS PUMP CALIBRATION nnnnn RDY DEGAS PRESSURE READING DEGAS CALIBRATION VALUE nnnnn Doc 031377 03 7 01 D 1 D TTL and Relay Control The strip of eight 2 pin connectors on the GP50 electronics chassis provides two relay outputs two TTL outputs and four TTL inputs see Figure D 1 To control functions in another device connect the GP50 TTL or relay outputs to the TTL or relay inputs of the device to be controlled See Section D 1 for details about GP50 TTL and relay output operation To have another device control GP50 functions connect the GP50 TTL inputs to the TTL outputs of the controlling device The following functions can be controlled pump motor on off method clock on off method number increment and method number decrement See Section D 2 for details about GP50 TTL input operation NOTE You can connect a non Dionex device to the GP50 provided it is compatible with the GP50 TTL and relay signals Refer to the user s manual for the device Refer to Section D 3 for connection instructions and examples Figure D 1 TTL Connector Strip GP50 front view without upper door GP50 Gradient Pump D 2 Doc 031377 03 7 01 D 1 TTL and Relay Output Operation The GP50 provides two TTL outputs and two relay contacts for controlling functions in external devices
54. GP50 GRADIENT PUMP OPERATOR S MANUAL 2001 Dionex Corporation Document No 031377 Revision 03 July 2001 2001 by Dionex Corporation All rights reserved worldwide Printed in the United States of America This publication is protected by federal copyright law No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Dionex Corporation 1228 Titan Way Sunnyvale California 94088 3603 U S A DISCLAIMER OF WARRANTY AND LIMITED WARRANTY THIS PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND DIONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR CHANGES TRADEMARKS DX LAN is a trademark and PeakNet Self Regenerating Suppressor and SRS are registered trademarks of Dionex Corporation Tefzel is a registered trademark of E I duPont de Nem
55. Gradient Pump is designed to perform ion chromatography and BioLC applications only and should not be used for any other purpose If there is a question regarding appropriate usage contact Dionex 3 1 1 Degas Eluents Dionex strongly recommends degassing all eluents and storing them in reservoirs pressurized with filtered inert gas see Section 3 1 3 This helps prevent bubbles caused by eluent outgassing from forming in the eluent proportioning valves pump heads and the detector cell Degassed eluents and pressurized reservoirs are especially important when combining aqueous and nonaqueous components e g water and acetonitrile The GP50 with the optional vacuum degas pump assembly provides continuous on line vacuum degassing of up to four eluents If the GP50 is not equipped with the vacuum degas assembly manually vacuum degas eluents daily as described below and store them in pressurized reservoirs Degassing Eluents Manually 1 Prepare the eluent required for your application Pour it into a vacuum flask and attach the flask to a vacuum pump or water aspirator 2 Vacuum degas the eluent for 5 10 minutes by shaking or sonication 3 Remove the flask from the vacuum Do not allow water to flow from the aspirator back into the flask 4 Pour the degassed eluent into a pressurizable reservoir Be careful not to shake the eluent 5 Install end line filters and pressurize the reservoirs see Sections 3 1 2 and 3 1 3
56. Japanese for Help and error messages Help Message DISPLAY PANEL BACKLIGHT KEY ACTUATION SOUND ENTRY ERROR SOUND MEDIUM ON ON MODULE SETUP HELP LANGUAGE ENGLISH GP50 Gradient Pump C 12 Doc 031377 03 7 01 C 1 8 Pump Options Use the PUMP OPTIONS screen to specify the type of pump head installed and to set pump operation parameters Figure C 9 Pump Options Screen PUMP HEAD MATERIAL Select INERT for PEEK pump heads or SST for stainless steel pump heads PUMP HEAD VOLUME Select 100 L for standard bore pump heads or 25 L for microbore pump heads CONTROL MODE Sets the pump flow control mode PRESSURE mode uses pressure as the primary source of feedback to maintain flow rate stability FLOW mode uses motor speed as the primary source of feedback to maintain flow rate stability TTL2 OUTPUT USAGE Sets the active state of the TTL2 output signal Select 0 FLOW to automatically switch off the power to a Self Regenerating Suppressor when the pump flow stops To implement this feature TTL2 out on the pump must be connected to TTL3 in on the detector For detailed instructions see Section B 3 Select NORMAL to activate TTL2 as a standard TTL output PRESSURE UNITS Sets the unit of measure for pressure PSI MPa or BAR Help Message PUMP HEAD MATERIAL PUMP HEAD VOLUME CONTROL MODE INERT 100 uL PRESSURE PUMP OPTIONS TTL2 OUTPUT USAGE PRESSURE UNITS 0 FLOW PSI C
57. M L C L E A K F R O N T P A N E L RLY 1 OUT OUT RLY 2 TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN P6 CPU RLY SLOT 5 DIST Connect the serial interface cable from the LC30 here Connect the leak sensor cable from the LC10 or LC20 here Connect the solenoid valve cable from the LC10 LC20 LC25 or LC30 here B Installation Doc 031377 03 7 01 B 5 LC25 Connections Route the electronics injection valve cable from the rear of the LC25 Chromatography Oven through the GP50 upper chase see Figure B 1 Connect the cable to the LC AIR connector in slot 1 of the pump electronics chassis see Figure B 2 NOTE Do not connect the LC25 to the LC LEAK connector in slot 1 the oven contains internal leak control electronics LC30 Connections 1 Route the RJ 11 serial cable from the rear of the LC30 Chromatography Oven through the GP50 upper chase see Figure B 1 Connect the cable to the LC COMM connector in slot 1 of the pump electronics chassis see Figure B 2 2 Route the solenoid valve cable from the rear of the LC30 through the GP50 upper chase Connect the cable to the LC AIR connector in slot 1 see Figure B 2 NOTE Do not connect the LC30 to the LC LEAK connector in slot 1 the oven contains internal leak control electronics GP50 Gradient Pump B 6 Doc 031377 03 7 01 B 2 3 DX LAN Interface 10BASE T Connections Optional N
58. N TTL 3 IN TTL 4 IN RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN 1 2 3 4 5 PeakNet Chromatography Workstation DX LAN 6 7 8 9 10 11 12 Note The pump and detector TTL input signal modes must be set to NORMAL PULSE Ground Serial Comm Port Hold Run Hold Run Gradient Start Output Inject Hold Input Doc 031377 03 7 01 Index 1 Index Symbols A B C D C 3 C 7 Entering eluent percentages 3 7 Numerics 100 L pump heads 2 14 C 12 10BASE T DX LAN cable Installation B 8 10BASE T DX LAN interface 1 1 Installation B 6 B 9 Rear panel connector 2 18 25 L pump heads 2 14 C 12 60 pin ribbon cable 2 10 A Aborting a running method 3 12 Activating error sounds C 11 Activating key press sounds C 11 Actuator for main power switch 2 2 Air pressure requirements 3 2 Air solenoid valves 2 10 Alarm leak 4 7 Arrow buttons 2 6 AS3500 Autosampler D 7 AS40 Automated Sampler D 6 Automated control of GP50 1 1 2 18 B Backlight 2 2 C 11 Adjusting brightness C 11 Time in use C 16 BAR C 12 Battery disposing 5 1 Binary curve gradients 3 19 Blank fields in method step 3 8 Blank screen 2 2 C 11 BNC DX LAN interface 1 1 Installation B 9 B 11 B 12 Rear panel connector 2 18 BNC tee connector installation B 11 Bottles See Reservoirs Button descriptions 2 4 2 6 C C curve column in Metho
59. ODE NORMAL EDGE TTL TTL TTL TTL DECREMENT METHOD NUMBER GP50 Gradient Pump D 4 Doc 031377 03 7 01 The four input signal modes are Normal Edge In normal edge operation the negative falling edge of a signal turns on the function and the positive rising edge turns off the function see Figure D 4 For example a negative edge sent to TTL1 turns on the pump motor and a positive edge turns off the motor Inverted Edge The inverted edge mode works identically to the normal edge mode except that the positive and negative edges are reversed in function Normal Pulse In normal pulse operation the negative falling edge of the TTL signal is the active edge and the positive rising edge is ignored For example applying a negative pulse to TTL1 when the pump motor is off turns the motor on This has the same result as pressing the Off On button on the front panel keypad The minimum pulse width guaranteed to be detected is 50 ms The maximum pulse width guaranteed to be ignored as noise or invalid is 4 milliseconds The action of the GP50 is undefined for pulses less than 50 ms or greater than 4 ms Inverted Pulse The inverted pulse mode operates identically to the normal pulse mode except that the positive and negative edges are reversed in function Figure D 4 TTL and Relay Input Signal Modes D TTL and Relay Control Doc 031377 03 7 01 D 5 D 3 TTL and Relay Connections The TTL relay connector strip i
60. OTE Check the DX LAN connector on the GP50 rear panel If a 10BASE T RJ 45 telephone style connector is installed follow the instructions in Section B 2 3 However if a BNC connector is installed follow the instructions in Section B 2 4 In order to communicate with a host computer running Dionex chromatography software the GP50 must contain a pump interface card P N 056800 and an unshielded twisted pair 10BASE T DX LAN cable P N 960281 must be connected from the 10BASE T RJ 45 connector on the rear panel to a combo 10BASE T Ethernet hub P N 056909 Installing or Replacing the Pump Interface Card NOTE If the DX LAN option was not installed at the factory order the pump interface card kit P N 057005 The kit includes all the components required for DX LAN communication STATIC The GP50 electronics cannot be serviced by the user The pump interface card must be installed by qualified personnel Standard anti static procedures must be observed when installing the interface card or handling the CPU card To prevent damage to the GP50 turn off the main power before installing the pump interface card After confirming that the LED on the CPU card is off not green or red unplug the power Do not rely on the front panel power switch Pour viter d endommager le GP50 coupez l alimentation lectrique principale avant d installer la carte interface du pompe Apr s avoir confirm que la DEL de la carte d unit centr
61. Options Screen Help Message PUMP HEAD MATERIAL PUMP HEAD VOLUME CONTROL MODE INERT 100 uL PRESSURE PUMP OPTIONS TTL2 OUTPUT USAGE PRESSURE UNITS 0 FLOW PSI B Installation Doc 031377 03 7 01 B 21 the GP50 and into the detector upper chase Plug the other end of the cable into TTL3 IN on the detector see Figure B 7 maintaining the wire orientation described in Step 4 Figure B 7 TTL Connections for SRS Power Control Detector and pump front views without front doors GP50 Gradient Pump B 22 Doc 031377 03 7 01 Doc 031377 03 7 01 C 1 C Display Screens This appendix illustrates and describes all of the screens that can be displayed on the GP50 front panel There are three types of screens operational diagnostic and calibration see Figure C 1 Operational screens allow you to select default pump operating parameters and to create edit and run methods that use timed events to control pump operation Diagnostic screens provide access to diagnostic information and tests Calibration screens allow you to calibrate pump functions flow rate pressure offset etc Figure C 1 GP50 Menu Structure GP50 Gradient Pump C 2 Doc 031377 03 7 01 C 1 Operational Screens C 1 1 Menu of Screens The MENU of SCREENS provides top level access to GP50 display screens There are two ways to select a screen from a menu Press the numeric button on the front panel keypad that corre
62. Status C 18 Elapsed Time C 16 Keyboard Test C 20 Leak Status C 27 Power Up screen C 15 Pressure Calibration C 30 See also Calibration Menu Diagnostic Test screen C 21 Digital Signal Processor DSP 1 1 C 17 See also DSP Dimensions A 1 Dionex Contacting 4 1 Direct control mode 3 6 Description 2 18 Selecting 3 5 Display and keypad specifications A 1 Display screens 2 7 Backlight 2 2 C 11 Calibration Status C 26 Degas Pump Calibration C 34 Degas Status C 28 Detail Status C 5 Diagnostic screens C 14 C 25 Diagnostic Test C 21 DSP Code Version C 24 DSP Status screen C 17 DX LAN Status C 18 Elapsed Time C 16 Leak Status C 27 Main status C 3 Menu of Screens C 2 Method edit C 6 Method edit extension screen C 8 Module Setup screen C 11 Power Up 2 7 3 3 C 15 Pressure Calibration C 30 Pump Options C 12 Time Function In C 13 Distribution card 4 3 4 9 4 10 Accessing 5 11 DSP card 2 9 Troubleshooting 4 5 4 6 4 8 DSP program 1 1 2 15 Error messages 4 5 4 6 Status C 17 Testing C 22 DSP Status screen 4 4 C 17 DX LAN 10BASE T cable See 10BASE T DX LAN cable DX LAN coaxial cable See Coaxial DX LAN cable DX LAN interface 1 1 2 10 2 18 2 20 A 3 10BASE T connections B 6 BNC connections B 9 Card description 2 10 Diagnostic test C 22 Rear panel connector 2 18 DX LAN Status screen C 18 E E01 Eluent Organizer 2 17 Editing a method 3 11 Editing a running method
63. TL and Relay Connections D 5 D 3 1 Example Connections D 6 Doc 031377 03 7 01 1 1 1 Introduction 1 1 Overview The GP50 Gradient Pump is an integral part of a Dionex chromatography system It is a microprocessor based dual piston variable speed gradient delivery system designed to blend and pump mixtures of up to four different eluents at precisely controlled flow rates The pump can deliver the selected eluent composition isocratically or as a multistep linear or curved gradient A Digital Signal Processor DSP provides high speed control of pump flow and pressure The pump can operate as a stand alone product or with other Dionex modules as part of a complete chromatography system It can also be used with non Dionex modules that meet interface requirements for software TTL or relay control The GP50 can be controlled locally using the front panel keypad and display or from a remote host computer with a Dionex DX LAN interface installed and PeakNet software installed on the host computer Limited remote control is also available from any device capable of providing compatible TTL signals to control the pump The pump s two basic modes of control Direct control and Method control enable it to operate with or without reference to time based events The GP50 is available in four versions An
64. TO RUN 0 2 2 TIME A L 2 00 INIT 0 00 PSI B C D V gt gt gt gt 100 0 _ 5 00 100 0 10 00 65 0 35 0 100 0 3 Operation and Maintenance Doc 031377 03 7 01 3 17 6 Move the cursor to the next line Enter 15 in the TIME field Move the cursor to the B field and enter 50 followed by 50 in the C field Beginning with the method step immediately preceding this one TIME 10 the pump begins adding eluent C starting with 0 When the method reaches this step after 15 minutes eluent C is at 50 eluent B at 50 and eluent A at 0 7 Move the cursor to the next line Enter 25 in the TIME field Move the cursor to C and enter 100 After a total of 25 minutes the concentration of eluent B drops to 0 and the concentration of eluent C increases to 100 8 Move the cursor to the next line Enter 25 01 in the TIME field Move the cursor to D and enter 100 The concentration of eluent C drops to zero and the concentration of eluent D which the pump began adding 0 6 seconds 0 01 min earlier reaches 100 This is a step change in eluent composition to 100 eluent D A step change is a very steep linear gradient in which the eluent composition changes from one eluent to another in 6 seconds Figure 3 10 illustrates the METHOD screen as it appears after Step 8 9 Move the cursor to the next line Enter 30 in the TIME field Move the cursor to the D field and enter 100 The eluent
65. The method number 0 through 99 to edit SAVE TO The method number 0 through 99 to save the current method to RUN The method number 0 through 99 to run Entering a method number in this field does not affect the status of the method clock The Hold Run button on the front panel keypad controls the clock LIMITS The low and high pressure limits in psi MPa or bar See Section 3 1 5 for guidelines for selecting pressure limits Select the unit of measure from the PUMP OPTIONS screen see Section C 1 8 TIME The elapsed time for each method step Every method must begin with the INIT initial step followed by the TIME 0 00 step Each additional entry under TIME indicates the elapsed time at which the specified conditions eluent percentages curve number flow rate etc occur Help Message 25 0 FLOW 0 5 METHOD EDIT LIMITs 0 5000 SAVE TO RUN 10 06 05 TIME A L 1 00 I INIT 0 00 345 67 PSI V 123 45 2 00 gt gt gt gt L 100 0 10 0 17 2 25 0 0 0 22 2 19 6 25 0 0 0 32 3 33 2 25 0 0 0 35 5 30 0 C B C D C Display Screens Doc 031377 03 7 01 C 7 A B C D The percentage of each eluent occurring at the start of the step The total of all eluents must equal 100 C The gradient curve type 0 through 9 to use when moving from the previous step to the current step See Section 2 9 4 for an explanation of the curve types Because ther
66. adient Pump 4 4 Doc 031377 03 7 01 Low pressure limit was tripped The following message is displayed 1 Verify that eluent is present in the channel selected If the eluent reservoir is empty refill it or select a channel which does have eluent Prime the pump see Section B 2 8 before resuming operation 2 Make sure the waste valve on the pressure transducer see Figure B 5 is closed by turning the knob on the pressure transducer housing clockwise 3 Make sure there are no liquid leaks in the flow system 4 Set the pump to LOCAL mode DIRECT CONTROL Press Off On to start the pump Verify that the pistons are moving and that you can hear the pump If there is no sound from the pump check the LED on the CPU card inside the door to the electronics chassis A red LED indicates a defective power supply To have the power supply P N 046440 replaced contact Dionex Technical Support With the pump running open the DSP STATUS screen press Menu 8 8 and 3 and note whether the left right pressure varies by more than 3 between strokes If it does refer to Section 4 1 If it does not either increase the flow rate or reduce the low pressure limit setting and continue operation Low Pressure Limit Violation Overtightening the pressure transducer waste valve may damage the valve and the pressure transducer housing 4 Troubleshooting Doc 031377 03 7 01 4 5 High pressure limit was tripped The following messa
67. al est teinte ni verte ni rouge d branchez le courant Ne vous fiez pas la position de l interrupteur d alimentation du panneau avant B Installation Doc 031377 03 7 01 B 7 1 To replace an existing pump interface card remove the DX LAN cable from the 10BASE T DX LAN connector on the GP50 rear panel see Figure B 1 2 Remove any TTL Relay plugs from the connectors at slot 4 of the electronics chassis see Figure B 2 3 Disconnect the 60 pin ribbon cable from the inside of the front panel by opening the ejector latches on the connector 4 Using a screwdriver as a lever open the white ejector latch at the bottom of the CPU card Remove the CPU card cable and Relay card as a single unit 5 Remove the pump interface card if present from slot 4 of the electronics chassis 6 Insert the new pump interface card P N 056800 into slot 4 a Slide the card to the rear b Verify that the 10BASE T connector is aligned with the opening at the rear and that the card is aligned with the card connector c Press firmly on the card until it mates fully with the connector on the rear panel Pull on the card to verify that it cannot move 7 Reinstall the CPU Relay card Press firmly until the card is inserted into the connector on the rear panel 8 Reconnect the ribbon cable to the 60 pin connector on the inside of the front panel The header and connector are key polarized near the center The ejecto
68. ated offset value FLOW Current flow rate Help Message OPEN PRESSURE TRANSDUCER WASTE VALVE PRESSURE CALIBRATION THEN SELECT OFFSET CAL AND ENTER READY SLOPE PRESSURE CAL MODE PSI OFFSET FLOW 1 00 m L MIN PSI 0 900 450 CAL VALUE 2500 PSI C Display Screens Doc 031377 03 7 01 C 31 4 When the pressure reaches zero select OFFSET CAL and press Enter The offset calibration begins When calibration is complete the screen displays the following The PSI OFFSET field displays the calibrated value The SLOPE field does not change and the PRESSURE field displays the new pressure based on the calibrated offset 5 There are three choices for continuing Save the PSI offset calibration and maintain the current slope value To implement Press Menu to exit the PRESSURE CALIBRATION screen The new offset value will take effect Save the PSI offset calibration and save the default slope value displayed in the SLOPE field To implement Press Enter to save the default slope value The PRESSURE field displays the new pressure based on the calibrated offset and the default slope Save the PSI offset calibration and calibrate the slope To implement Press a Select button to select SLOPE CAL in the CAL MODE field and press Enter Continue to the next section Calibrating the Slope Figure C 26 Pressure Calibration Screen Second View Help Message ENTER TO USE SLOPE DEFAULT
69. ation points are the points at which the pump speed is adjusted at each stroke to maintain a constant flow rate Help Message DSP STATUS PRESSURIZATION POINTS LEFT RIGHT nnn nnn TACH VALUE 5 00 V HEAD MATERIAL INERT HEAD VOLUME 100uL PRESSURE FLOW MODE EN FOUND nnnn PSI OFF YES GP50 Gradient Pump C 18 Doc 031377 03 7 01 C 2 5 DX LAN Status Use the DX LAN STATUS screen to monitor several types of errors that may occur on the network Figure C 15 DX LAN Status Screen UNIQUE DX LAN ID Displays the three byte DX LAN ID programmed in the Moduleware in HEX DX LAN ADDRESS Displays the six byte DX LAN address assigned by the PC in HEX The first three bytes are the system assignment and the last three bytes are the assignment within the system COLLISION Indicates that 16 unsuccessful transmissions of the same packet occurred due to collisions BUS WRITE Indicates that a ready response could not be issued within 2 4 microseconds after the WR signal was asserted This occurs when the transmit buffer memory is full UNDERFLOW Indicates that data from the transmit section of the hardware buffer memory is not available for serial transmission The DX LAN will continue to send out this data frame ALIGNMENT Indicates that a packet was received with an alignment error meaning that there were one to seven extra bits at the end of the packet This is usually caused by a collision or a fau
70. ch end of the BNC DX LAN network Before beginning operation verify that both ends of the DX LAN have terminator resistor plugs installed B Installation Doc 031377 03 7 01 B 13 B 2 5 Waste Lines The GP50 requires two waste lines one line from the pressure transducer assembly and one from the leak drain These lines are factory installed and routed to the rear panel Place the free end of both waste lines into a single waste container Position the waste container below the level of the GP50 to maintain a positive siphon see Figure B 3 Figure B 3 Eluent Waste Lines Rear Panel Waste Container Leak Line Waste Line GP50 Gradient Pump B 14 Doc 031377 03 7 01 B 2 6 Eluent Inlet Line Connections Eluent lines A through D are plumbed to the pump at the factory To complete the installation attach the free end of each line to the corresponding eluent reservoir B 2 7 Eluent Outlet Line Connections Connect the eluent line exiting the pressure transducer see Figure 5 1 to the gradient mixer Connect the tubing exiting the gradient mixer to the injection valve B Installation Doc 031377 03 7 01 B 15 B 2 8 Piston Seal Wash Connections Optional NOTE To install the piston seal wash order the piston seal wash kit P N 059187 Follow the instructions below or the duplicate instructions provided in the kit to set up the seal wash 1 Fill a wash bottle with deionized water and place
71. chromatography module Column B is available only when a column switching valve is installed TTL1 TTL2 RLY1 RLY2 Provides TTL and relay control of other devices Select 1 on or 0 off Appendix D describes TTL and relay control Help Message COL RLY1 A A METHOD EDIT TTL OUT SAVE TO RUN 10 06 05 TIME INIT 0 00 345 67 v 123 45 gt gt gt gt RLY OUT B TTL1 TTL2 RLY2 0 1 1 0 1 0 0 1 1 C Display Screens Doc 031377 03 7 01 C 9 C 1 6 Degas Options Use the DEGAS OPTIONS screen to set the duration and frequency of the vacuum degas pump cycles NOTE The DEGAS PUMP CALIBRATION screen displays the degas threshold value see Section C 3 7 Figure C 7 Degas Options Screen DEGAS PUMP Specifies how the vacuum degas pump operates BY SETTING The degas pump runs according to the options selected from this screen MONITOR The GP50 monitors the degas vacuum reading every one minute If the reading falls 500 counts below the calibration threshold value the degas pump turns on and runs for the cycle duration time ALWAYS OFF The pump is always off ALWAYS ON The pump is always on This setting is reserved for test purposes by Dionex Service Representatives START UP DURATION The length of time the pump runs at power up 2 5 min CYCLE DURATION The length of time the pump runs during each cycle 0 120 sec TIME BETWEEN CYCLES The time
72. cm 2 in Black PEEK Tubing P N 057057 After starting the flow insert Barbed Fittings 4 P N 037431 Syringe P N 054578 Draw to start the flow Pump Heads Elevate bottle above the pum p B Installation Doc 031377 03 7 01 B 17 B 2 9 Priming the Pump This section describes three priming procedures The first two Priming with a Syringe and Priming with the PRIME Button are standard procedures If these are ineffective try the third procedure Priming with Isopropyl Alcohol Priming with a Syringe NOTE Priming with a syringe is recommended after changing eluents or when eluent lines are empty 1 Press MENU and Enter to go to the MAIN screen Check that the operating fields are set to LOCAL and DIRECT CNTRL If REMOTE or METHOD is currently set move the cursor to the appropriate field press Select or Select to toggle the value and press Enter or a cursor arrow button 2 Move the cursor to the desired eluent Enter 100 and press Enter or a cursor arrow button This selects 100 of the eluent and automatically sets other eluents to 0 3 Connect a 10 mL syringe P N 054578 to the luer port in the priming block see Figure B 5 NOTE If the eluent is pressurized liquid will flow into the syringe as soon as you open the priming block valve in the next step Do not pressurize above 55 KPa 8 psi 4 Turn the priming block valve counterclockwise about two turns 5 Press Off O
73. composition remains unchanged at 100 eluent D for 4 99 minutes 10 Move the cursor to the next line and enter 30 01 in the TIME field Move the cursor to the A field and enter 100 This causes another step gradient from 100 D to 100 A Figure 3 10 Linear Gradient Method Example After Step 8 Help Message C FLOW METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 2 2 TIME A 2 00 PSI B C D V gt gt gt gt 10 00 65 0 35 0 gt 15 00 25 00 25 01 50 0 50 0 100 0 100 0 GP50 Gradient Pump 3 18 Doc 031377 03 7 01 11 Move the cursor to the SAVE TO field and press Enter to save the method to memory Figure 3 11 illustrates the completed method 12 Press MENU and Enter to go to the MAIN screen Enter 2 in the METHOD field Press Reset to reset the method to the INIT step if necessary 13 If the pump motor is off press Off On to start the pump 14 If the method clock is in hold press Hold Run to start the method running When the method reaches the last step in the method TIME 30 01 the pump will continue to pump isocratically until the clock is reset Figure 3 11 Linear Gradient Method Example Complete Help Message C FLOW METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 2 2 TIME A 2 00 PSI B C D V gt gt gt gt gt 25 00 25 01 100 0 100 0 30 00 30 01 100 0 100 0 3 Operation and Maintenance Doc 031377 03 7 0
74. creen displays see Figure 2 4 A series of diagnostics tests is run at power up If one of the tests fails the DIAGNOSTIC TEST screen displays instead See Section C 2 7 if this occurs When the GP50 power is turned on the injection valve is initialized to the Load position 2 Press Off On to start the pump flow 3 Check the pressure reading on the MAIN screen The GP50 display updates the pressure readout once per piston stroke The reading from one stroke to the next should be within 3 A variation of more than 3 indicates that the pump is out of prime Refer to Section B 2 8 for priming instructions or see Section 4 1 for other conditions which can cause the pump to lose prime NOTE After starting the pump or changing the flow rate wait at least 5 minutes up to 20 minutes for low flow rates in a standard bore pump before beginning an analysis This allows the pump s real time electronic pulse damping circuitry to stabilize the flow rate GP50 Gradient Pump 3 4 Doc 031377 03 7 01 3 1 5 Selecting the Pressure Limits The high and low pressure limits automatically stop the pump if a system malfunction occurs such as overpressurization caused by a blockage or low pressure caused by a leak downstream from the pump When PeakNet is controlling the pump select the pressure limits from the software When the pump is running under Local mode Direct control enter the pressure limits on the DETAIL screen see Figur
75. ct Control Commands are sent from PeakNet 5 and executed immediately when received Locked Remote Direct Control Commands are sent from PeakNet 5 or PeakNet 6 and executed immediately when received Remote Method Commands are sent from PeakNet 5 and executed by running a method programmed in PeakNet 5 GP50 Gradient Pump 2 20 Doc 031377 03 7 01 Remote Mode In Remote mode PeakNet 5 software sends operating commands from the host computer via the DX LAN interface In Remote mode operating parameters can be changed from the front panel provided they do not interfere with a running method Locked Remote Mode In Locked Remote mode PeakNet 5 or PeakNet 6 software sends operating commands from the host computer via the DX LAN interface In Locked Remote mode all operating changes from the front panel are disabled When the GP50 is controlled by PeakNet 5 select the Locked Remote Start option from the PeakNet 5 Run program to operate in the Locked Remote mode To return the GP50 to Local mode clear the Start option or turn the GP50 power off and then on When the GP50 is controlled by PeakNet 6 connecting to the PeakNet 6 timebase automatically selects the Locked Remote mode To return the GP50 to Local mode either clear the Connect check box on the PeakNet 6 control panel or turn the GP50 power off and then on 2 9 3 Method Control In Method control commands are executed according to the time based steps programmed
76. d For more accurate readings abort the method go to the MAIN screen and select Direct Control mode see Section C 1 2 Then re open the PRESSURE STATISTICS screen Figure C 18 Pressure Statistics Screen Active Method Lowers MIN MAX Resolution PRESSURE The measured pressure from the pressure transducer MAX The maximum pressure value during the duration of the test MIN The minimum pressure value during the duration of the test DURATION The duration of the test The test starts upon entering this screen and terminates upon exiting by pressing the Menu button To restart the test press Reset the duration is set to 0 and all status values are set to the current pressure FLOW MODE Reports the flow mode as ON or OFF ON System is in constant flow mode OFF System is in pressure feedback mode P POINT LEFT RIGHT Reports the current pressurization point for the left and right pistons The GP50 updates the readout once per piston stroke Help Message PRESSURE PRESSURE STATISTICS MAX nnnnn nn PSI RIGHT FLOW MODE MIN P POINT LEFT ON nnn MINUTES DURATION nnnnn nn PSI nnnnn nn nnnnn nn PSI nnn Active Method Lowers MIN MAX Resolution GP50 Gradient Pump C 24 Doc 031377 03 7 01 C 2 9 DSP Code Version Use the DSP CODE VERSION screen to view the current DSP digital signal processor code revision numbers Figure C 19 DSP Code Version Screen Help Message DSP COD
77. d screen 3 7 C 7 Cables 10BASE T DX LAN B 6 B 8 60 pin ribbon 2 10 Air solenoid valves 2 10 Coaxial DX LAN B 9 B 11 Front panel 60 pin ribbon cable 2 10 LC10 LC20 leak sensor 2 9 B 4 LC10 LC20 LC25 LC30 solenoid valves B 5 LC30 serial interface 2 9 B 5 Power cord B 3 Calibration Menu C 25 Calibration Status C 26 Degas Pump Calibration C 34 Degas Status C 28 Flow Calibration C 29 Leak Sensor Calibration and Status C 27 Pressure Calibration C 30 See also Diagnostic screens Calibration procedures Degas pump C 34 Flow rate C 29 Leak sensor C 27 Pressure offset and slope C 30 C 33 GP50 Gradient Pump Index 2 Doc 031377 03 7 01 Cards See Printed circuit boards Cascading hubs B 9 CE Mark 1 4 Check valves 3 2 Cartridges 5 3 Cause of pressure fluctuations 4 2 Cleaning 5 1 Description 2 15 Replacing 5 1 5 3 Chromatography software 1 1 PeakNet 5 2 18 2 19 PeakNet 6 2 18 2 19 See also PeakNet 5 software and PeakNet 6 software Circuit boards See Printed circuit boards Clock See Method clock Coaxial DX LAN cable Installation B 11 B 12 COL A B field in Method screen C 8 Column switching valve Selecting C 3 C 8 Computer control 1 1 2 18 See also Chromatography software See also PeakNet 5 software and PeakNet 6 software Concave linear curve 2 23 Connectors Electronics chassis 2 8 LC Air valves 2 10 LC Leak 2 9 LC30 Communication 2 9 B 5 TTL Relay control
78. d step parameters press any button except Delete Turns the method clock off Hold and on Run This button functions only when the pump is under Method control see Section 2 9 3 When the method clock is in Hold pressing Hold Run starts the clock at either the initial step of a new method or if resuming an interrupted method at the time at which the clock was put in Hold When the method clock is in Run pressing Hold Run stops the clock this holds the method and freezes the current conditions Changes the method clock time to INIT causing the initial conditions specified by the method to occur This button functions only when the GP50 is under Method control see Section 2 9 3 If the method is running it continues running If the method is in Hold the method clock executes the initial conditions and holds When the cursor is positioned at a field with predetermined parameters these buttons cycle through the options In fields with predetermined numeric values pressing Select increases the value by one unit pressing Select decreases the value by one unit Holding down a Select button increases or decreases the value continuously Press Enter or a cursor arrow button to confirm the selected value Keypad Buttons Function Table 2 1 GP50 Front Panel Buttons Continued GP50 Gradient Pump 2 6 Doc 031377 03 7 01 The four cursor buttons move the cursor in the direction of the arrow to the next
79. ded twisted pair cable Do not substitute a cable of an inferior grade Failure to use the correct cable will cause the pump to lose communication with the host computer When using 10BASE T cabling you must install a hub If you simply plug the 10BASE T cable from the pump into the connector on the host computer s DX LAN card the connection will either not work or will be unreliable B Installation Doc 031377 03 7 01 B 9 Cascading Hubs Cascading or connecting hubs together through their 10BASE T ports increases the number of ports or the number of users supported on the network For instructions refer to the installation guide for the hub B 2 4 DX LAN Interface BNC Connections Optional NOTE Check the DX LAN connector on the GP50 rear panel If a BNC connector is installed follow the instructions in Section B 2 4 However if a 10BASE T RJ 45 telephone style connector is installed follow the instructions in Section B 2 3 In order to communicate with a host computer running Dionex chromatography software the GP50 must contain a pump interface card P N 046341 and a coaxial DX LAN cable P N 960405 must be connected to the BNC tee connector P N 921914 on the rear panel From the GP50 connect the DX LAN cable to Another instrument equipped with a BNC connector or A combo 10BASE T Ethernet hub P N 056909 The hub has one BNC port and eight 10BASE T RJ 45 ports Installing or Replacing the P
80. e wait at least 5 minutes up to 20 minutes for low flow rates in a standard bore pump before beginning an analysis This allows the flow rate to stabilize In the A B C and D columns enter decimal percentage values from 0 1 through 100 for the eluent compositions The combined percentages for all eluents must total 100 or the pump will not run In the V column select the injection valve position L for load or I for inject In the FLOW column enter the pump flow rate Flow rates are adjustable in increments of 0 01 mL min See Section 2 4 2 for the available flow rate ranges They vary depending on the size of the pump head and whether the run is isocratic or gradient For steps other than INIT and TIME 0 0 enter a curve number in the C column The curve number determines whether the pump delivers a linear or curved Continuous operation of the microbore pump at flow rates above 2 0 mL min will decrease seal and pump life For optimum performance above 2 0 mL min a standard bore pump should be used GP50 Gradient Pump 3 8 Doc 031377 03 7 01 gradient when moving to the step from the one preceding it see Section 2 9 4 The default is curve 5 linear NOTE Because there are no previous steps for INIT or TIME 0 0 curve numbers are not entered for them If a step field is blank has no entry the last selected value for the field remains in effect The symbol next to the bottom time entry indicates
81. e 3 1 When the pump is running under Local mode Method control enter the pressure limits on the METHOD screen see Figure 3 5 The limits are set in the INIT step and remain unchanged throughout the analysis When a limit trip stops the pump the method clock immediately stops and goes to Hold The current status of the program that was running at the time is displayed on the front panel To select the pressure limits from the front panel 1 Go to the DETAIL or METHOD screen and move the cursor to the LIMIT field 2 Enter a low pressure limit between 1 4 and 2 8 MPa 200 400 psi The setting may vary depending on the system operating pressure The low pressure limit is activated after 13 pump piston strokes i e after 1 3 mL standard bore or 0 325 mL microbore of fluid is pumped through 3 Enter a high pressure limit that is 2 8 to 3 4 MPa 400 500 psi above the normal system operating pressure The pump is equipped with a pressure limit that prevents operation above 35 MPa 5076 psi Figure 3 1 Detail Screen Setting Pressure Limits Help Message 2 00 2125 LOCAL COLUMN DETAIL SCREEN 100 0 A TTL1 TTL2 RLY1 RLY2 0 0 1 1 INJECT 300 2500 PSI 0 0 B 0 0 C 0 0 D LIMIT DIRECT CNTRL PSI mL MIN SAMPLE A 3 Operation and Maintenance Doc 031377 03 7 01 3 5 3 1 6 Selecting the Operating and Control Modes 1 Go to either the MAIN or DETAIL screen The operating mode
82. e a parameter position the cursor in the field and enter the new value The previous value is automatically deleted To add a method step move the cursor to any TIME field and press Insert or move the cursor to the empty TIME field below the last step and enter the elapsed time at which to start the new step When you press Enter or a cursor arrow button the new step is automatically moved to the correct chronological position Continue entering parameters for the new step To delete a method step move the cursor to the step to be deleted and press Delete twice 3 When changes are complete move the cursor to the SAVE TO field Press Enter to save the changes to the current method or enter a new method number and press Enter If you save changes to the currently running method they are immediately incorporated in the run and executed at the programmed time unless the modified event has already been executed To restart the method at the INITial conditions and run all steps of the updated method press Reset GP50 Gradient Pump 3 12 Doc 031377 03 7 01 3 3 4 Deleting a Method To delete an entire method move the cursor on the METHOD screen to the INIT step and press Delete twice 3 3 5 Changing the Running Method To change from the method currently running to a different method go to the MAIN or DETAIL screen enter the new method number in the METHOD field and press Enter The new method will begin running using
83. e and seal a Fill the head cavity with deionized water by injecting through either the piston opening or the inlet check valve b Reinsert the piston approximately 1 8 in into the seal see Figure 5 4 c Install a 10 32 fitting plug P N 042772 on the outlet check valve Tighten the plug d Push the piston into the head This should hydraulically unseat the seal and piston guide from the head Remove the piston and pull off the guide and seal NOTE If the piston guide and seal do not come out make sure the 10 32 plugs are tight Then add more water and repeat Steps b and d e Remove the 10 32 fitting plug Figure 5 4 Removing the Piston Seal Outlet Check Valve Inlet Check Valve Fitting Plug Optional Backup Washer Remove Piston Seal Piston Guide Pump Head Push in to unseat the seal and piston guide Remove the piston from the head and pull off the seal and piston guide 10 32 Fitting Plug P N 042772 5 Service Doc 031377 03 7 01 5 7 8 To install the new seal and reinstall the piston guide a Push the piston through the piston guide and the new seal Then insert the piston piston guide and seal into the pump head until the seal contacts the bottom of the counterbore see Figure 5 5 View A b Hold the piston guide and seal in place and remove the piston from the head see Figure 5 5 View B c Seat the seal by pushing the piston guide into the head
84. e are no previous steps for INIT or TIME 0 0 you cannot enter curve numbers for these steps V The position of the injection valve either I inject or L load FLOW The flow rate through the pump in milliliters per minute v The symbol next to the bottom time entry indicates there are additional steps below the last step in the screen Move the cursor to the bottom time entry and press the down arrow to see the additional step s The symbol next to the top time entry indicates there are additional steps above the top line Move the cursor to the top time entry and press the up arrow to see the additional steps s gt The symbol gt at the right edge of each line indicates a lateral extension to the line Move the cursor to the end of a line and press the right arrow to display the METHOD extension screen see Section C 1 5 GP50 Gradient Pump C 8 Doc 031377 03 7 01 C 1 5 Method Extension Screen NOTE The METHOD extension screen can be accessed only from the METHOD screen see Section C 1 4 To return to the METHOD screen move the cursor to the left edge of the screen and press the left arrow Use the METHOD extension screen to select the active column and the TTL relay output parameters for each step in the method NOTE The METHOD EDIT SAVE TO and RUN fields are the same as in the METHOD screen Figure C 6 Method Extension Screen COL Column Selects the active column A or B in the
85. e can cause the following problems leaks nonreproducible eluent compositions which may cause retention time shifts and flow restrictions which may cause high backpressure 1 Turn off the main power switch to ensure you do not unintentionally start the GP50 2 Turn off the pressure on the eluent reservoirs and allow them to vent 3 Follow these steps to disconnect the proportioning valve electrical connector from the distribution card a Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door Pull out the drawer a few inches b The distribution card is on the top of the mechanical chassis Labels printed on the card identify the various cables plugged into it Locate the proportioning valve electrical connector labeled VALVES at the front of the card Disconnect it from the card 4 Disconnect the liquid line from the manifold outlet see Figure 5 7 Figure 5 7 Location of Proportioning Valve Manifold Assembly Manifold Outlet 1 2 3 4 Proportioning Valve Manifold Assembly P N 046203 GP50 Gradient Pump 5 12 Doc 031377 03 7 01 5 Loosen the screws securing the proportioning valve manifold assembly to the bulkhead see Figure 5 7 Pull the valve manifold assembly forward and disconnect the eluent lines on the manifold inlet Rem
86. e on the eluent If pressurization is used provide a source of helium to pressurize the eluent and regenerant reservoirs if used House eluents at least 3 cm 8 in above the pump in an EO1 Eluent Organizer P N 044125 or in built in eluent containment such as the LC25 Chromatography Oven reservoir container Always filter eluents to remove small particulates that may contaminate the pump Install an end line filter P N 045987 on the end of each eluent reservoir line Filters are supplied in the pressurizable reservoir Ship Kits Refer to the Pressurizable Reservoir Installation Instructions for details Dionex strongly recommends degassing eluents If the vacuum degas assembly is not installed refer to Section 3 1 1 for manual degassing instructions Lift the GP50 only from the bottom or side surfaces of the module Lifting by the front doors will damage the door hinges Use caution when lifting the module which weighs 19 kg 42 lb Ne soulevez le GP50 que par le fond ou les c t s Son soul vement par la porte du panneau avant endommagera les charni res de la porte Soyez prudent lorsque vous soulevez le GP50 il p se 19 kg 42 lb GP50 Gradient Pump B 2 Doc 031377 03 7 01 B 2 Installation Instructions The GP50 Ship Kit contains items necessary for completing the installation B 2 1 Power Connection GP50 Gradient Pump Version Ship Kit Standard bore microbore with PEEK components P N 054621 Standard b
87. e the piston guide see Figures 5 3 and 5 4 6 The pistons are captured by a magnetic retention system and do not come off as part of the pump head assembly After removing the pump head hold the shaft of the piston and apply just enough lateral force to overcome the magnetic field and remove the piston Lateral motion while disengaging the head from the piston may break the piston Un mouvement lat ral pendant la s paration de la t te et du piston peut casser le piston 5 Service Doc 031377 03 7 01 5 5 Figure 5 3 Pump Head Assembly Component Standard Bore PEEK Standard Bore SST Microbore PEEK Microbore SST Pump Head Assembly 054087 054096 054094 054095 Pump Head 054051 054050 054098 054099 Outlet Check Valve 047661 047665 047657 047663 Inlet Check Valve 047660 047665 047656 047663 Piston Seal 054400 054402 054401 054401 Piston Guide 045633 045633 045632 045632 Rinse Seal 048722 048722 048721 048721 Support Washer 050745 050745 050744 050744 O Ring 014895 014895 014895 014895 Backup Washer 045630 045630 045631 045631 Piston 052840 052840 053584 053584 Table 5 1 Pump Head Assembly Part Numbers Inlet Check Valve Assembly Backup Washer Piston Head O Ring Piston Seal Piston Guide Rinse Seal Support Washer Outlet Check Valve Assembly GP50 Gradient Pump 5 6 Doc 031377 03 7 01 7 To remove the piston guid
88. eck the entire mechanical chassis for leaks from the rinse ports the eluent manifold connections and valves the vacuum degas chambers and the eluent reservoirs see Figure 3 20 Tighten or replace any leaking fittings Wipe up liquid spills and rinse dried reagents off the pump components with deionized water Dionex recommends thoroughly flushing the vacuum degas assembly with deionized water after each use Flushing chemicals out of the degas chambers and tubing avoids crystallization in the membrane pores Figure 3 20 Eluent Flow Schematic Eluent Reservoirs Pressure Transducer Mixer To Column Rinse Ports Priming Block Pump Heads VAC Chamber IN VAC Chamber OUT OUT Proportioning Valve Manifold Assembly A B C D A B C D 3 Operation and Maintenance Doc 031377 03 7 01 3 27 3 5 2 Periodic Maintenance Replace both the primary and rinse seals in each pump head approximately every 6 months The seals may need to be replaced more often if you routinely run high pressures or high flow rates or if you operate the pump continuously 3 6 Shutdown Rinse the pump piston seals before and after daily operation to prevent build up of salt crystals or other contaminants that can damage the piston seals see Section 3 5 1 Before a shutdown of three days or more flush the system with deionized water to prevent contaminants from building up Or if this is not possible maintain a continuo
89. ement 5 11 5 12 Testing C 21 PSI C 3 C 12 Pump configurations 2 14 Pump eluent manifold 2 13 Pump heads 2 14 Assembly drawing 5 5 Assembly part numbers 5 5 Specifying material type C 12 Specifying volume 25 100 L C 12 Pump interface card Installation procedure B 6 B 7 B 9 B 10 Pump mixers 2 14 Pump motor control 2 4 Pump Options screen B 20 C 12 Pump priming block 2 15 Pump versions 1 1 Index Doc 031377 03 7 01 Index 9 R Rear panel 2 18 B 3 10BASE T DX LAN interface B 8 BNC DX LAN interface B 11 Fuses 5 13 Power receptacle B 3 Waste lines B 13 Regulator 3 2 Relay connectors 2 10 See also TTL Relay control Remote mode 2 19 C 4 Description 2 18 2 20 Locked Remote 2 20 Selecting 2 18 3 5 Reservoirs Installation B 1 B 14 Installation in EO1 2 18 B 1 Pressurizing 3 2 Storing eluents 3 1 Types of 2 17 Reset button 2 5 Rinse ports leaks from 4 2 4 7 4 12 5 4 5 8 Rinsing Piston seals 3 25 3 27 Piston seals continuously 2 16 3 25 B 15 Pump components 3 27 System continuously during shutdown 3 27 Routine maintenance 3 25 Daily 3 25 Running methods 2 5 3 10 Editing while running 3 23 S Safety labels 1 4 Safety messages 1 3 Salt crystals 2 16 3 25 Save to field Method screen C 6 Schematic eluent flow 2 13 Screen brightness C 11 Screen contrast 2 2 Screens See Display screens Seal replacement 5 4 Select buttons 2 5 Self Regene
90. ents can cause dirty or defective check valves Install end line filters P N 045987 to help prevent this see Section 3 1 2 c Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door Pull out the mechanical chassis drawer a few inches d If a piston does not move when there is pump flow examine it for breakage and replace if necessary If a piston moves examine the pump head for scratches and replace if necessary see Section 5 3 If a piston moves slightly and then breaks contact with the rocker arm follower replace the piston seal see Section 5 2 e Push the mechanical chassis drawer back in place making sure the cables are not pinched Tighten the drawer lock Observe the warning label on the inside of the lower door The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is on and the mechanical drawer is open Do not touch any parts within the mechanical chassis while the pump is on Respectez l tiquette d avertissement appos e l int rieur de la porte inf rieure Les fl ches sur l tiquette indiquent des pi ces m caniques mobiles qui posent un danger de pincement lorsque le GP50 est sous tension et le tiroir m canique est ouvert N utilisez jamais le GP50 avec le tiroir du ch ssis m canique ouvert 4
91. essure transducer waste valve should remain open GP50 Gradient Pump B 20 Doc 031377 03 7 01 7 Press Prime to purge the alcohol from the pump heads and flush the heads with the desired eluent 8 Close the pressure transducer waste valve 9 Set the desired flow rate The pump is now ready for operation B 3 Automatic SRS Power Control Optional TTL connections can be used to automatically switch off the power to a Self Regenerating Suppressor SRS when the GP50 flow stops In this setup the GP50 sends a TTL signal to the detector and then after a 5 minute delay the detector shuts off the SRS power The 5 minute delay allows momentary flow interruptions without disturbing the SRS 1 Go to the GP50 PUMP OPTIONS screen and set TTL2 OUTPUT USAGE to 0 FLOW see Figure B 6 2 Go to the detector TTL FUNCTION IN screen and assign TTL3 to SRS OFF ON 3 Locate a pair of twisted black and red wires P N 043598 and two green TTL connector plugs P N 921019 in the GP50 Ship Kit 4 Insert the wires into the plugs The signal wire red goes on top and the ground wire black goes on the bottom of each plug 5 Plug one end of the cable into TTL2 OUT on the GP50 The TTL connectors are located behind the upper door Route the cable through the upper chase of Isopropyl alcohol may damage some columns Be sure to thoroughly rinse the alcohol from the pump using the process described in Step 7 Figure B 6 Pump
92. ext to the test name press Select or Select to select the asterisk and press Enter The table below explains the test status indicators NOTE Exiting the DIAGNOSTIC TEST screen clears the pass fail indicators from the edit fields Character Test Status _ Test did not run gt Test is in progress P Test passed F Test failed POWER Checks the 5 15 and 24 volt monitor on the relay board CPU Checks the CPU internal configuration and the Moduleware checksum DX LAN Checks the DX LAN hardware configuration and loop back LOOP COM Checks the LC30 Chromatography Oven communication hardware A loop back cable must be plugged into the oven communication port The pump then runs a loop back test on the port DSP COM Checks communication between the pump CPU and the DSP Digital Signal Processor hardware by sending a command and waiting for the appropriate response PUMP LEAK Checks the pump s leak sensor for a correct open circuit or short circuit condition EXTERNAL LEAK Checks the external leak sensor for a correct open circuit or short circuit condition C Display Screens Doc 031377 03 7 01 C 23 C 2 8 Pressure Statistics Use the PRESSURE STATISTICS screen to view statistical data about the pressure transducer The values are updated while the screen is displayed If a method is running when you go to the PRESSURE STATISTICS screen the following message is displaye
93. first two steps can be changed but the steps cannot be deleted Enter the parameters for both steps 6 Enter a new step using one of the following methods Move the cursor to the empty TIME field below the last step and enter the elapsed time at which to start the new step Press Enter or a cursor arrow button Move the cursor to any of the TIME fields and press Insert This adds a new step after the cursor position Enter the elapsed time at which to start the new step and press Enter or a cursor arrow button After entering a new step all timed steps are automatically organized in chronological order 7 Continue entering parameters for the new step When you finish move the cursor to the SAVE TO field and do one of the following If you are editing an existing method enter a new number for the method and press Enter If you are editing an unused method press Enter Figure 3 5 Method Screen Creating a New Method Help Message C FLOW _ METHOD EDIT LIMITs 0 5000 SAVE TO RUN 0 5 5 TIME A L 1 00 INIT 0 00 PSI B C D V gt gt gt gt 100 0 _ GP50 Gradient Pump 3 10 Doc 031377 03 7 01 3 3 2 Running a Method 1 If the pump motor is off press Off On to turn on the motor 2 Go to the MAIN or DETAIL screen and if necessary change the operating and control modes to LOCAL and METHOD see Section 3 1 6 3 In the METHOD field enter the desired method number If t
94. fittings may strip the threads in the manifold block If this happens replace the valve manifold assembly 5 Service Doc 031377 03 7 01 5 13 5 6 Changing Main Power Fuses 1 Turn off the main power 2 The fuse holder is part of the main power receptacle on the rear panel see Figure 5 8 Note the recessed lock located on each side of the fuse holder Using a small screwdriver push each lock toward the center to release it When both locks are released and the fuse holder pops out slightly pull the fuse holder straight out of its compartment 3 Replace the two fuses in the holder with new IEC127 fast blow fuses rated 3 15 amps P N 054745 Dionex recommends always replacing both fuses 4 Reinsert the fuse holder into its compartment The fuse holder is keyed to fit only in its proper orientation Apply enough pressure evenly against the holder to engage the two locks When both locks are engaged the holder is flush against the panel 5 Reconnect the main power cord and turn on the power HIGH VOLTAGE Disconnect the main power cord from its source and also from the rear panel of the GP50 HAUTE TENSION D branchez le cordon d alimentation principal de sa source et du panneau arri re du GP50 Figure 5 8 Main Power Fuse Holder GP50 Gradient Pump 5 14 Doc 031377 03 7 01 Doc 031377 03 7 01 A 1 A Specifications A 1 Electrical A 2 Environmental A 3 Physical A 4 Display and
95. ge is displayed 1 Isolate segments of the flow path to determine the source of the high backpressure a Remove the pump inlet tubing from the injection valve b Turn on the pump and record the backpressure c One at a time add each segment of the remainder of the flow path to determine the source of the backpressure If reconnecting a component causes a sharp increase in backpressure preplace the component Replace tubing fittings or components as necessary to resume operation at the standard operating backpressure d If the source of the high backpressure is the column refer to the column manual for cleanup procedures The column may need replacement 2 Verify that the pressure transducer is calibrated correctly a Open the pressure transducer waste valve see Figure B 5 by turning the knob counterclockwise about two turns b Check the pressure reading if it is above 97 KPa 14 psi recalibrate the pressure transducer see Section C 3 6 A DSP related error message is displayed Several error messages are related to Digital Signal Processor DSP errors DSP communication fails DSP does not acknowledge etc If one of these messages is displayed follow the procedure below 1 Turn off the GP50 power 2 Verify that the DSP card is present and correctly installed in slot 1 of the electronics chassis see Figure 2 6 3 Turn on the GP50 power High Pressure Limit Violation GP50 Gradient Pum
96. he method clock is already running the method starts immediately If the clock is in Hold press Hold Run to start the method NOTE You can also select the method number in the METHOD screen Move the cursor to the RUN field and enter the desired method number 4 The elapsed time on the method clock when the method begins determines where at what step and parameters the method begins running If the method clock is at INIT or time zero the method begins running using the INITial condition parameters If the method clock is greater than zero the method begins running using the parameters specified in the step for that elapsed time To start the method at the INITial conditions instead press Reset 3 Operation and Maintenance Doc 031377 03 7 01 3 11 3 3 3 Editing a Method Existing methods can be modified by changing adding or deleting steps and parameters Changes can be made when the method clock is stopped or running If the method you are editing is currently running the changes are stored in memory and implemented when you save the method NOTE After saving changes there is no way to recall the original method If you plan to make experimental changes to a method but also want to retain the original method save the modified method to a new number To edit a method 1 Go to the METHOD screen In the EDIT field enter the number of the method to be modified 2 Make the required changes To chang
97. ion 5 5 Doc 031377 03 7 01 5 1 5 Service This chapter describes service and repair procedures that the user may perform Any procedures not included here including electronics related service procedures must be performed by Dionex personnel For assistance contact Dionex Technical Support In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex office Before replacing any part refer to the troubleshooting information in Chapter 4 to isolate the cause of the problem The CPU card contains a lithium battery If the CPU card is displaced dispose of the used battery according to the manufacturer s instructions 5 1 Cleaning and Replacing the Check Valves A dirty check valve causes erratic flow rates and pressures and may cause the pump to lose prime and or be difficult to reprime 1 Turn off the main power switch to ensure you do not unintentionally start the pump 2 Disconnect the tube fittings from the inlet and outlet check valve housings see Figures 5 1 and 5 2 3 Use a 1 2 inch wrench to loosen both check valve housings Remove the check valve housings and cartridges from the pump head Carefully remove the check valve cartridges from the housings 4 Place the check valve housings and cartridges in a beaker with methanol and sonicate or agitate for several minutes Substituting non Dionex parts may impair performance thereby voiding the product warranty Refer to the warranty s
98. ion screen C 29 Flow column in Method screen 3 7 Flow mode C 12 Flow rate Calibrating C 29 Calibration Status screen C 26 Ranges 2 14 A 2 Selecting C 3 Setting in Method screen 3 7 C 7 Stabilizing 3 7 Flow schematic 2 13 Front panel 2 2 Display screens 2 7 Keypad 2 3 Tilting 2 2 Front panel buttons Description 2 4 2 6 Functional description 2 18 Method control 2 20 Fuses A 1 Replacing 5 13 G Gas regulator 3 2 Generating a method 3 8 GM 4 Gradient Mixer 2 14 GM 5 Gradient Mixer 2 14 Gradient control specifications A 3 Index Doc 031377 03 7 01 Index 5 Gradient curves 2 22 Concave 2 23 Convex 2 23 Linear 2 23 Gradient eluent run 2 22 Curved gradient example 3 19 Linear gradient example 3 14 H Heads See Pump heads Helium 3 2 Help messages Language selection C 11 Hold Run Front panel button 2 5 Host computer 1 1 2 18 2 20 Connecting B 6 B 9 Hub 10BASE T B 6 B 8 B 9 Humidity A 1 Hydraulic specifications A 2 I Important icon 1 3 INIT initial conditions 2 5 3 9 Injection valve Connection B 4 Controlling position of C 3 Inlet line connection B 14 Insert button 2 4 Inserting Method steps 2 4 Installation B 1 10BASE T DX LAN interface B 6 BNC DX LAN interface B 9 CPU 2 10 Eluent line connections to pump B 14 Eluent outlet line B 14 Eluent reservoirs 2 17 Facilities required B 1 LC air valves B 5 LC leak 2 9 B 5 LC10 LC20 connecti
99. ional backpressure tubing or adjust the flow rate to achieve this backpressure Increasing the flow rate increases the pressure decreasing it decreases the pressure When the gauge reading has stabilized at the recommended value enter the reading in the CALVALUE field Column If a column is installed the pressure to use for calibration is determined by the application flow rate and the type of column installed After allowing the pressure to stabilize enter the reading in the CALVALUE field NOTE The pressure gauge readings may fluctuate slightly as the left and right pump heads alternate piston strokes You can ignore variations of about 0 03 MPa 5 psi or less Larger fluctuations generally indicate that the pump is out of prime If this occurs prime the pump heads see Section B 2 8 and then repeat Step 5 6 Press Enter The slope calibration begins When calibration is complete the screen fields display the following The SLOPE field displays the new slope value The PRESSURE field displays the same value as the CALVALUE GP50 Gradient Pump C 34 Doc 031377 03 7 01 C 3 7 Degas Pump Calibration Use the DEGAS PUMP CALIBRATION screen to calibrate the degas pump Figure C 29 Degas Pump Calibration Screen CALIBRATE DEGAS THRESHOLD To calibrate select CAL and press Enter After calibration is complete the entry reverts to RDY DEGAS PRESSURE READING Reports the current degas pressure reading D
100. ions between the GP50 and other modules CPU Control Moduleware for the pump resides on the CPU Relay cards The CPU logic and Relay I O cards occupy slot 5 The Relay I O card rides piggyback on the CPU card and extends over the front of slot 4 The card is short enough to allow the optional DX LAN pump interface card P N 044195 to mount behind it in slot 4 A 60 pin ribbon cable links the CPU logic to the display and keypad The logic monitors the internal power supply outputs and reports the status on the multicolored LED at the bottom of slot 4 Green indicates normal operation Red indicates a power fault The GP50 will enter its diagnostic state and inhibit all other controls until the fault is corrected If this occurs turn off the power for a few seconds and then turn it back on 2 Description Doc 031377 03 7 01 2 11 2 3 Mechanical Chassis The mechanical chassis is housed in a pull out drawer located behind the lower door of the pump enclosure The front of the chassis contains the components described in Section 2 4 Other mechanical assemblies are located inside the chassis drawer The drawer should be pulled out only for service procedures For routine operation push in the drawer and tighten the lock located on the lower right corner of the chassis Observe the warning label on the inside of the lower door The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is o
101. k valves were serviced Reset this field to 0 when the valves are serviced RESET Resets the SEALS IN USE or VALVES IN USE counter to 0 Move the cursor to the corresponding asterisk field and press Enter Always reset the seals and valves counters to 0 when they are replaced or serviced Help Message ELAPSED TIME MODULE ON nnnnnnnn hours BACKLIGHT nnnnnnnn hours PUMP CYCLES nnnnnnnn cycles SEALS IN USE nnnnnnnn cycles VALVES IN USE nnnnnnnn cycles RESET C Display Screens Doc 031377 03 7 01 C 17 C 2 4 DSP Status Use the DSP STATUS screen to check the status of DSP digital signal processor dynamic parameters Status updates are in real time Figure C 14 DSP Status Screen PRESSURE Reports the current head pressure in psi MPa or bar TACH VALUE Reports the current motor tachometer reading in volts FLOW MODE Reports the flow mode as ON or OFF ON System is in constant flow mode OFF System is in pressure feedback mode HEAD MATERIAL Reports the head material specified on the PUMP OPTIONS screen see Section C 1 8 INERT PEEK heads METAL stainless steel heads EN FOUND Reports whether the encoder is found HEAD VOLUME Reports the head volume specified on the PUMP OPTIONS screen The options are 100 L for standard bore heads or 25 L for microbore heads PRESSURIZA TION POINTS Reports the current pressurization point for the left and right pistons The pressuriz
102. l to its vertical position Power Switches The main power switch is on the bulkhead behind the upper door see Figure 2 1 An actuator for the main power switch is on the outside of the front door at the lower left corner see Figure 2 2 The actuator functions only when the door is fully closed When the door is open press the main power switch on the bulkhead to turn the module off and on To prevent damage to the pump circuitry and components always wait at least 15 seconds after powering down before turning on the power again 2 Description Doc 031377 03 7 01 2 3 2 1 1 Control Panel Keypad Use the keypad to directly control pump operation as well as to create and modify programmed series of timed events called methods In summary Press Menu to display a list of available screens To go from a menu to a screen press the numeric button that corresponds to the screen s number on the menu or move the cursor to the desired screen name and press Enter Only fields shown in reverse video on a screen can be edited Other fields display information only To edit a value in a reverse video field use the four directional arrow buttons to move the cursor to the field Use the numeric buttons to Figure 2 2 GP50 Display and Keypad Layout GP50 Gradient Pum p Hold Run 1 0 2 3 4 5 6 7 8 9 Enter Off On Prime Reset Insert Select Delete Select Help Menu Tab for opening
103. leaks 8 Reconnect the liquid lines 9 Turn on the GP50 power 10 Prime the pump see Section B 2 8 If the pump will not prime and you have eliminated all other possible causes of the problem replace the check valve cartridge After replacing the check valve cartridge go to the ELAPSED TIME screen press Menu 8 and 2 Move the cursor to the VALVES IN USE field and press Enter to reset the field to 0 cycles Overtightening may damage the pump head and check valve housing and crush the check valve seats Check Valve Cartridge Type Part Number Standard Bore PEEK 047747 Standard Bore Stainless Steel 047755 Microbore PEEK 047748 Microbore Stainless Steel 048279 GP50 Gradient Pump 5 4 Doc 031377 03 7 01 5 2 Piston Seal Replacement A damaged seal allows leakage past the piston and then through the rinse ports in the front of the pump heads The pump may be difficult to prime flow rates will be unstable and baseline noise may be observed 1 Turn off the main power switch to ensure you do not unintentionally start the GP50 2 Disconnect the tube fittings from the pressure transducer and the inlet check valve see Figures 5 1 and 5 2 3 Remove the two nuts from the pump head 4 Carefully disengage the head from the piston by pulling the head straight off and away from its mounting guides 5 Place the head front end down on a clean work surface and lift off the backup washer to expos
104. length of each method and the amount of available memory thus the actual total may be less than 100 Help Message 25 0 FLOW 0 5 METHOD EDIT LIMITs 0 5000 SAVE TO RUN 10 06 05 TIME A L 1 00 I INIT 0 00 345 67 PSI V 123 45 2 00 gt gt gt gt L 100 0 10 0 17 2 25 0 0 0 22 2 19 6 25 0 0 0 32 3 33 2 25 0 0 0 35 5 30 0 C B C D GP50 Gradient Pump 2 22 Doc 031377 03 7 01 2 9 4 Eluent Delivery Isocratic Eluent Run The simplest use of the GP50 is for the delivery of an isocratic unchanging mixture of one or more eluents If more than one eluent is selected the pump delivers a proportional mixture of the eluents based on the percentage of each eluent selected The combined percentages of all eluents selected must total 100 or the pump will not run Gradient Eluent Run The GP50 can produce step linear concave or convex curves in eluent concentration over a specified time period The slope of the gradient is determined by the selected gradient curve see Figure 2 10 and the time between the starting and ending points of a gradient step It is important to note the following points The curve number parameter determines whether the pump delivers a linear or curved gradient Figure 2 10 Eluent Composition Profile for Curves 1 9 1 2 3 4 5 6 7 8 9 1 0 9 8 7 6 5 4 3 2 1 0 0 1 2 3 4 5
105. leshooting 4 2 4 4 4 7 4 8 LED Power supply status 2 10 Left right pressurization points C 23 Limit field Detail screen C 5 Method screen C 6 Linear gradient curve 2 23 Linear gradient example 3 14 Liquid crystal display LCD Adjusting the screen contrast 2 2 Liquid leaks See Leaks Liquid lines PEEK 5 2 Stainless steel 5 2 Local mode 2 19 Commands allowed 2 19 Description 2 18 Selecting 2 18 2 20 3 5 Locked Remote mode 2 19 2 20 C 4 Description 2 18 Selecting 3 5 C 4 M Main status screen C 3 Maintenance 3 25 Daily 3 25 Periodic 3 27 Replacing piston seals 3 27 Routine maintenance 3 25 Manifold 2 13 Menu Accessing 2 6 Selecting options 2 6 Menu button 2 6 Menu of Screens C 2 Menu structure chart C 1 Method clock 2 21 Controlling 3 12 Controlling with TTL input D 3 Starting 2 5 Starting a method 3 10 Stopping 2 5 Method control 2 21 3 7 Description 2 19 Selecting 3 5 Method edit extension screen C 8 Method edit screen C 6 Method number C 4 Index Doc 031377 03 7 01 Index 7 Method steps Adding 2 4 Blank fields in 2 5 3 8 Definition 2 21 Deleting 2 5 Entering 3 7 C 6 Inserting 3 9 Maximum number allowed 2 21 Restoring the previous value 2 5 Setting step times C 6 Methods Aborting a running method 3 12 Control 3 7 Creating 3 8 Curved gradient example 3 19 Deleting a method 3 12 Deleting a step 3 11 Editing 3 11 3 23 Examples 3
106. low rate through the pump in milliliters per minute In Direct control set the flow rate in this field In Method control program the flow rate on the METHOD screen see Section C 1 4 Help Message PSI LOAD METHOD 100 0 A 0 0 B 0 0 C 0 0 D COLUMN A 2000 mL MIN 2 00 LOCAL 2 MIN 10 27 GP50 Gradient Pump C 4 Doc 031377 03 7 01 MIN Displays the total elapsed time that the method clock has been running The value can be changed When you enter a new elapsed time the method settings corresponding to the new time go into effect LOCAL REMOTE LOCKED RMT Selects the operating mode Local mode is used to control GP50 operation from the front panel Remote mode is used when PeakNet 5 is running the GP50 Locked Remote mode is always used when PeakNet 6 is running the GP50 and is an option when PeakNet 5 is running the GP50 In this mode operation from the front panel is disabled To change to Local mode use one of the following methods From PeakNet 6 clear the Connect check box on the PeakNet 6 control panel From PeakNet 5 clear the Locked Remote Start option in the Run program Turn off the GP50 power and turn it on again DIRECT CONTROL METHOD Selects the control mode Direct control is used to execute operating commands immediately Method control is used to run a programmed series of timed events Enter the method number to run in the field C Display Screens
107. lty transceiver Help Message UNIQUE ID DX LAN STATUS LAN ADDRESS COLLISION ALIGNMENT UNDERFLOW BUS WRITE nnn nnn nnn nnn FF FF FF FF FF FF FF FF FF RETRY CRC OVERFLOW BUS READ nnn nnn RUNT nnn nnn nnn C Display Screens Doc 031377 03 7 01 C 19 BUS READ Indicates that a ready response could not be issued within 2 4 microseconds after the ready signal was asserted This occurs when reading an empty buffer OVERFLOW Indicates that the DX LAN hardware receive buffer became full and had to reject a packet for lack of space CRC Indicates that a packet was received with a CRC error This usually means that a collision has corrupted the packet RUNT Indicates that a runt packet one less than 15 bytes in length was received This usually occurs after a collision has truncated the original length RETRY Indicates the number of retries required to transmit the last packet GP50 Gradient Pump C 20 Doc 031377 03 7 01 C 2 6 Keyboard Test Use the KEYBOARD TEST screen to conduct an interactive test of the front panel keypad buttons To test the buttons 1 Press a button on the keypad Its display changes to reverse video confirming proper operation of that button 2 Continue pressing all buttons in turn Only the most recently pressed button shows in reverse video 3 To end the test and return to the DIAGNOSTIC MENU press Menu twice Figure C 16 Ke
108. mp and other Dionex chromatography modules Figure 2 6 shows the electronics components with the upper door open To open the door pull on the tab located to the right of the main power actuator see Figure 2 2 Figure 2 5 Menu of Screens Do not remove any of the electronics cards from the pump There are no user serviceable components on the cards If servicing is required it must be performed by qualified personnel and appropriate electrostatic discharge ESD handling procedures must be followed Ne retirez aucune des cartes lectroniques de la pompe Aucun des composants sur les cartes ne peut tre r par par l utilisateur Toute r paration doit tre effectu e par un personnel qualifi utilisant des proc dures correctes de d charge lectrostatique Help Message MAIN SCREEN DETAIL SCREEN METHOD DEGAS OPTIONS MODULE SET UP PUMP OPTIONS TIME FUNCTION IN DIAGNOSTIC MENU 1 2 3 4 5 6 7 8 MENU of SCREENS 2 Description Doc 031377 03 7 01 2 9 LC LEAK The leak control cable from the LC10 Chromatography Organizer or LC20 Chromatography Enclosure connects to the LC LEAK connector in slot 1 When a leak occurs in the LC10 or LC20 it is reported to the GP50 NOTE The LC25 Chromatography Oven and LC30 Chromatography Oven do not connect to the GP50 LC LEAK connector These ovens contain internal leak control electronics LC COMM The LC30 Chromatography Oven s RJ 11 serial cable connects
109. n 1 Turn off the main power switch to ensure you do not unintentionally start the GP50 2 Disconnect the tube fittings from the pressure transducer and the inlet check valve see Figures 5 1 and 5 2 3 Remove the two acorn nuts from the pump head Lateral motion while disengaging the head from the piston may break the piston Un mouvement lat ral pendant la s paration de la t te et du piston peut casser le piston 5 Service Doc 031377 03 7 01 5 9 4 Slowly pull the head and allow it to separate from the housing Carefully disengage the head from the piston by pulling the head straight off and away from the mounting guides 5 The pistons are captured by a magnetic retention system and do not come off as part of the pump head assembly After removing the pump head hold the shaft of the piston and apply just enough lateral force to overcome the magnetic field and remove the piston 6 Replace the piston and the piston seal Broken or scratched pistons can damage the piston seal 7 Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself To do this a Press the backup washer into the head until it is flush with the indented surface of the head b Insert the piston halfway into the head This ensures that the magnet in the follower picks up the piston The follower is the cylinder that holds the piston in place as it moves in and out of the pump head
110. n and the mechanical drawer is open Do not operate the pump with the mechanical chassis drawer pulled out Respectez l tiquette d avertissement appos e l int rieur de la porte inf rieure Les fl ches sur l tiquette indiquent des pi ces m caniques mobiles qui posent un danger de pincement lorsque le GP50 est sous tension et le tiroir m canique est ouvert N utilisez jamais le GP50 avec le tiroir du ch ssis m canique ouvert GP50 Gradient Pump 2 12 Doc 031377 03 7 01 2 4 Mechanical Components Figure 2 7 shows the mechanical components located behind the lower door of the enclosure Figure 2 7 GP50 Mechanical Components Pressure Transducer Waste Valve Pressure Transducer Priming Block Pump Heads Eluent Manifold Proportioning 2 Description Doc 031377 03 7 01 2 13 2 4 1 Pump Eluent Manifold Eluent lines A through D are routed through the rear of the GP50 to the vacuum degas pump if installed see Section 2 5 and then to the rear of the proportioning valve If the vacuum degas pump is not installed the eluent lines are connected directly to the proportioning valve The proper proportion of eluent exits the front of the valve and is directed to the priming block Figure 2 8 shows the eluent flow path through the system Figure 2 8 Eluent Flow Schematic Eluent Reservoirs Pressure Transducer Mixer To Column Rinse Ports Priming Block Pump Heads VAC Chamber IN
111. n to turn on the pump motor 6 If the eluent is not pressurized make sure there is a vent from the eluent bottle or loosen the bottle caps 7 Draw the syringe back to pull eluent through the flow path It may take several syringe draws to remove all air or previous eluents from the tubing NOTE If the GP50 includes a vacuum degas assembly draw an additional 17 mL of eluent through the priming block GP50 Gradient Pump B 18 Doc 031377 03 7 01 8 Return to Step 2 Select another eluent and repeat the priming procedure 9 When the manifold has been primed with each eluent turn the priming block valve counterclockwise until closed see Figure B 5 Do not overtighten 10 Press Off On to turn off the pump motor The pump is now ready for operation Priming with the Prime Button 1 Go to the MAIN screen and select DIRECT CNTRL and LOCAL 2 Set the eluent percentage of the line being primed to 100 Press Enter or a cursor arrow button 3 Open the pressure transducer waste valve one to two turns counterclockwise This will direct the eluent flow path to waste and eliminate backpressure Figure B 5 Priming the Pump Pressure Transducer Waste Valve Priming Block Valve Syringe P N 054578 Priming Block Luer Port B Installation Doc 031377 03 7 01 B 19 4 Press Prime on the GP50 front panel The pump will pump at 9 9 mL min for a standard bore pump and 2 5 mL min for a microbore pum
112. nnn PSI nnn C Display Screens Doc 031377 03 7 01 C 27 C 3 3 Leak Sensor Calibration and Status Use the LEAK SENSOR CALIBRATION AND STATUS screen to check the status of leak sensor parameters and to calibrate the sensor The PUMP column displays the status of the pump s internal leak sensor The EXTERNAL column displays the status of the leak sensor in the chromatography module If no chromatography module is attached to the pump the EXTERNAL fields display NONE Figure C 22 Leak Sensor Calibration and Status MEASURED VALUE The current measured voltage from the sensor CURRENT CONDITION The current condition of the leak sensor dry wet or err error The error condition indicates an open or short circuit To calibrate the sensor press a Select button to select CAL and press Enter The current measured value becomes the new dry calibration value CALIBRATION VALUE The value saved when the sensor was last calibrated LOW LEAK THRESHOLD The minimum voltage reading that will be accepted as indicating the leak sensor is dry a reading below this voltage indicates that the sensor is wet The threshold value is based on the calibration value Help Message MEASURED VALUE CURRENT CONDITION 2 48 LEAK SENSOR CALIBRATION AND STATUS CALIBRATION VALUE W ET PUMP EXTERNAL LOW LEAK THRESHOLD 2 75 DRY 2 50 2 60 2 70 2 50 GP50 Gradient Pump C 28 Doc 031377 03 7 01 C 3 4 Degas Status
113. ons B 4 LC25 connections B 5 LC30 connections B 5 Power connection B 2 Priming the manifold B 17 Pump interface card B 6 B 7 B 9 B 10 Rear panel 2 18 Setting pressure limits 3 4 TTL Relay connections 2 10 D 5 Waste line B 13 Internal leak sensor C 27 Introduction 1 1 Inverted edge D 4 Inverted pulse D 4 Isocratic eluent run 2 22 3 13 J Japanese On screen messages C 11 K Key press sound C 11 Keyboard Test screen C 20 Keypad 2 3 2 6 Keypad buttons 2 3 Delete 2 5 Enter 2 6 Key press sound C 11 Menu 2 6 Numerical keys 2 6 Off On 2 4 Prime 2 4 Reset 2 5 Select 2 5 GP50 Gradient Pump Index 6 Doc 031377 03 7 01 L LC leak 2 9 B 5 LC10 Chromatography Organizer Connections to pump B 4 Leak control cable 2 9 Solenoid valve connections 2 10 LC20 Chromatography Enclosure Connections to pump B 4 Leak control cable 2 9 Solenoid valve connections 2 10 LC25 Chromatography Oven Built in eluent containment B 1 Connections to pump B 5 Solenoid valve connections 2 10 LC30 Chromatography Oven Connections to pump 2 9 B 5 Solenoid valve connections 2 10 Leak alarm 4 6 4 7 Leak sensor Calibration C 27 Status C 27 Testing C 22 Leak sensor cable LC10 LC20 2 9 Leak status screen C 27 Leaks Checking for 3 26 Mechanical chassis 4 8 Piston seals 4 12 Pressure transducer 4 7 Priming valve 4 7 Proportioning valve 4 7 Pump heads 4 7 Rinse ports 4 7 Troub
114. ore microbore with stainless steel components P N 054627 SHOCK HAZARD To avoid electrical shock a grounded receptacle must be used Do not operate or connect to AC power mains without an earthed ground connection DANGER D LECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant avec prise de terre Ne l utilisez pas et ne le branchez pas au secteur C A sans utiliser de branchement mis la terre The power supply cord is used as the main disconnect device Make sure the socket outlet is located near the GP50 and is easily accessible Le cordon d alimentation principal est utilis comme dispositif principal de d branchement Veillez ce que la prise de base soit situ e install e pr s du module et facilement accessible Operation at AC input levels outside of the specified operating voltage range may damage the GP50 B Installation Doc 031377 03 7 01 B 3 The GP50 power supply is auto sensing so no adjustment is required to select the line voltage There are two ways to control power to the GP50 Control from the GP50 To implement Connect a modular power cord IEC 320 C13 from the GP50 main power receptacle see Figure B 1 to a grounded single phase power source Use the GP50 power switch to turn the pump power on and off Control from the LC30 Chromatography Oven To implement Locate one of the IEC jumper cables P N 960748 provided in the LC30 Ship Kit Connect the jum
115. ot move 7 Reinstall the CPU Relay card Press firmly until the card is inserted into the connector on the rear panel 8 Reconnect the ribbon cable to the 60 pin connector on the inside of the front panel The header and connector are key polarized near the To prevent damage to the GP50 turn off the main power before installing the pump interface card After confirming that the LED on the CPU card is off not green or red unplug the power Do not rely on the front panel power switch Pour viter d endommager le GP50 coupez l alimentation lectrique principale avant d installer la carte interface du pompe Apr s avoir confirm que la DEL de la carte d unit central est teinte ni verte ni rouge d branchez le courant Ne vous fiez pas la position de l interrupteur d alimentation du panneau avant B Installation Doc 031377 03 7 01 B 11 center The ejector latches must be partially open to accept the cable connector 9 Install the hex nut BNC tee connector and coaxial DX LAN cable see the following section Installing the BNC Tee Connector and Coaxial DX LAN Cable 1 Install the BNC tee connector P N 921914 a Note the two small locking pins on either side of the BNC DX LAN connector on the rear panel see Figure B 1 b Push the tee connector onto the BNC connector on the rear panel and twist until the locking pins are fully engaged in the slots on the tee connector c Pull firmly on
116. ours amp Co PRINTING HISTORY Revision 01 May 1998 Revision 02 October 2000 Revision 03 July 2001 Doc 031377 03 7 01 i Contents 1 Introduction 1 1 Overview 1 1 1 2 About This Manual 1 2 1 2 1 Safety Messages and Notes 1 3 1 2 2 Safety Labels 1 4 2 Description 2 1 Front Control Panel 2 2 2 1 1 Control Panel Keypad 2 3 2 1 2 Display Screens 2 7 2 2 Electronics Chassis 2 8 2 3 Mechanical Chassis 2 11 2 4 Mechanical Components 2 12 2 4 1 Pump Eluent Manifold 2 13 2 4 2 Pump Heads 2 14 2 4 3 Pump Mixers
117. ove the valve manifold assembly completely from the bulkhead 6 Thread the bundle of electrical lines from the new valve manifold assembly through the bulkhead and up to the distribution card Connect the valve electrical connector to the VALVES connector on the distribution card 7 Connect the eluent lines to the new valve manifold assembly Make sure eluent lines A through D are in the appropriate valve ports Connect eluent line A to the valve marked 1 eluent B to the valve marked 2 and so on 8 Align the new assembly as shown in Figure 5 7 and mount it to the bulkhead Tighten the screws 9 Push the mechanical chassis drawer back in place making sure the cables are not pinched Retighten the drawer lock 10 Attach the liquid line from the priming block to the manifold outlet 11 To verify that each valve is functioning properly connect a syringe to the priming valve One at a time select 100 of each eluent valve and draw liquid through the valve After successfully drawing liquid through each valve turn off the pump 12 Next verify that no liquid passes through the valve when the pump is off Do not turn on the pump One at a time select 100 of each eluent No liquid should flow through the valve 13 Turn on the main power switch The pump is ready for normal operation Tighten liquid connections to the valve no more than fingertight plus one quarter turn Overtightening or crossthreading the valve
118. p NOTE The vacuum degas assembly contains 17 mL of eluent If this option is installed allow several extra minutes of prime time to purge air and previous eluent from the degas assembly 5 Allow the pump to prime until all air and previous eluents are purged and no air bubbles can be seen exiting the waste line If the eluent manifold has not been primed allow several extra minutes for the eluent to pass through the pump 6 Press Prime again to return to the flow rate selected for the analysis 7 Close the pressure transducer waste valve The pump is now ready for operation Priming the Pump Heads with Isopropyl Alcohol NOTE Prime the pump heads with isopropyl alcohol only if the two standard procedures described previously are unsuccessful 1 Connect a 10 mL syringe P N 054578 filled with isopropyl alcohol to the luer port in the priming block valve see Figure B 5 2 Open the pressure transducer waste valve see Figure B 5 by turning the knob counterclockwise about two turns 3 Turn the priming block valve counterclockwise about two turns see Figure B 5 4 Turn on the pump 5 Use the syringe to slowly push alcohol through the pump Avoid pushing any air trapped in the syringe through the pump Verify that alcohol with no trapped bubbles is traveling down the waste line from the transducer 6 Close the priming block valve Do not overtighten Disconnect the syringe from the priming block The pr
119. p 4 6 Doc 031377 03 7 01 4 If the DSP error message reappears notify Dionex Technical Support The power supply P N 046440 DSP card P N 045369 or CPU card P N 046340 may need to be replaced The following error message displays If the pump motor is in a runaway condition the motor automatically shuts off and the above error message is displayed Contact Dionex Technical Support The following error message displays 1 Turn off the GP50 power 2 Verify that the cables connected to the DSP card in the electronics chassis see Figure 2 6 are seated properly 3 Turn on the GP50 power If the error message reappears notify Dionex Technical Support Do not remove any of the electronic cards from the pump There are no user serviceable components on the cards If servicing is required it must be performed by qualified personnel and appropriate electrostatic discharge ESD handling procedures must be followed Ne retirez aucune des cartes lectroniques de la pompe Aucun des composants sur les cartes ne peut tre r par par l utilisateur Toute r paration doit tre effectu e par un personnel qualifi utilisant des proc dures correctes de d charge lectrostatique Motor Drive Fails Encoder index not found 4 Troubleshooting Doc 031377 03 7 01 4 7 4 4 Liquid Leaks Leak Alarm Leaks from the front rinse ports or rear of the pump head may indicate a defective piston seal 1 Replace
120. per cable from the GP50 main power receptacle to an IEC auxiliary receptacle on the LC30 rear panel Leave the GP50 power switch on continuously and use the LC30 main power switch to turn the pump power on and off Figure B 1 GP50 Rear Panel GP50 Gradient Pump B 4 Doc 031377 03 7 01 B 2 2 Electronics Chassis Connections The electronics chassis connections vary depending on which chromatography module is installed with the pump Refer to the appropriate section below LC10 or LC20 Connections 1 Route the leak sensor cable from the rear of the LC10 Chromatography Organizer or the LC20 Chromatography Enclosure through the GP50 upper chase see Figure B 1 Connect the cable to the LC LEAK connector in slot 1 of the pump electronics chassis see Figure B 2 2 Route the solenoid valve cable from the rear of the LC10 or LC20 through the GP50 upper chase Connect the cable to the LC AIR connector in slot 1 of the pump electronics chassis see Figure B 2 NOTE Refer to Appendix D for TTL and relay installation instructions Figure B 2 GP50 Electronics Chassis Located behind pump upper door POWER SUPPLY GREEN OK RED FAULT SLOT 3 BLANK I LAN 000K SLOT 4 BLANK SLOT 2 SLOT 1 GP50 IP25 DSP 130W PWR SPY 1 I I P7 D S T 3 P N 1 I I P8 D S T 2 P N MOTOR L C A R L C C O M
121. procedures to be performed by the user Any other maintenance procedures must be performed by qualified Dionex personnel 3 5 1 Daily Maintenance When using a combination of eluents which contain both salt or base and solvent rinse the piston seals frequently or continuously Eluent tends to crystallize as the solvent evaporates these crystals can abrade the pistons and cause the piston seals to leak Rinse the piston seals before and after operation each day as described in the following steps or install a continuous seal wash kit P N 059187 to rinse the piston seals continuously see Section B 2 8 1 Open the lower pump door and locate the two rinse ports on the front of each of the pump heads Figure 3 19 shows the rinse port connections 2 Install a rinse waste tube P N 054418 provided in the GP50 Ship Kit onto each head see Figure 3 19 Figure 3 19 Rinsing the Piston Seals Syringe P N 054578 Rinse Ports with Female Luer Adapters Rinse Waste Tubes GP50 Gradient Pump 3 26 Doc 031377 03 7 01 3 Place the end of each rinse waste tube into a waste receptacle Attach a small syringe P N 054578 containing 5 to 10 mL of deionized water to the rinse inlet female luer adapter on one of the pump heads 4 Inject deionized water into the fitting to rinse the pump heads 5 Repeat Steps 3 4 for the other pump head 6 Dispose of the waste water and close the door to the mechanical chassis Ch
122. put operation 2 19 4 11 Functions controlled by C 13 D 3 Signal modes D 3 D 4 TTL Relay control D 1 Connections for SRS power control B 20 Connectors 2 10 D 1 Does not operate 4 11 Example connections D 6 Input operation 2 19 D 3 Input signal modes D 3 Installation D 5 Output operation D 2 Setting outputs C 5 C 8 D 2 TTL input signal modes D 4 TTL2 output does not operate 4 11 TTL2 output usage B 20 C 12 TUV 1 4 Type of control See Operating mode U User interface C 1 User interface screens See Display screens V V valve column in Method screen 3 7 C 7 Vacuum degas pump assembly See Degas pump assembly Valves Check valves 5 1 Column switching C 3 C 8 Injection C 3 Pressure transducer waste valve 2 15 B 18 Proportioning 2 13 2 17 C 21 See also Names of valves W Waste lines Installation B 13 Piston seal wash B 15 Pressure transducer 2 15 Waste valve 2 15 B 18 Weight A 1
123. r latches should be partially open to accept the cable connector 9 Install the 10BASE T DX LAN cable see the following section GP50 Gradient Pump B 8 Doc 031377 03 7 01 Installing the 10BASE T DX LAN Cable 1 Install the combo 10BASE T Ethernet hub P N 056909 on a workbench or on the wall For installation instructions and site requirements refer to the installation guide shipped with the hub 2 Plug the 10BASE T DX LAN cable P N 960281 into a 10BASE T port on the front panel of the hub 3 Connect the other end of the cable to the 10BASE T DX LAN connector on the GP50 rear panel see Figure B 1 4 Connect a 10BASE T cable P N 960281 from a 10BASE T port on the hub to the 10BASE T port on the host computer s internal DX LAN card If the connection is via port 8 on the hub set the Normal Uplink push button to Normal NOTE For installation instructions for the host computer s internal DX LAN card see Installing the Dionex PeakNet 6 System Document No 031631 or Installing the Dionex PeakNet System Document No 034941 Network Upgrades If you upgrade the network from a BNC connector to a 10BASE T connector turn off the computer power briefly and then turn it on again to allow the host computer s internal Ethernet DX LAN card to detect the 10BASE T connector The Ethernet card searches for the type of installed cabling only at power up The 10BASE T DX LAN cable is a Category 5 unshiel
124. rating Suppressor SRS Automatic power shutoff 4 11 B 20 B 21 Service 5 1 Check valves 5 1 Fuses 5 13 Piston seal 5 4 Proportioning valve 5 11 Pump piston 5 8 Waste valve O ring 5 10 Setting pressure limits 3 4 Setting step times C 6 Shutdown 3 27 Signal modes for TTL input D 3 Solenoid valves Connecting to the LC10 20 25 30 2 10 B 4 Specifications Control modes A 3 Display and keypad A 1 Electrical A 1 Environmental A 1 Hydraulic A 2 Physical A 1 Vacuum degas assembly A 3 SRS See Self Regenerating Suppressor SRS Stabilizing the flow rate 3 7 Stainless steel liquid lines 5 2 Standard bore heads 2 14 See also Pump heads Starting methods 2 5 Step See Method steps Step gradient change 3 17 Stopping methods 2 5 System outlet line B 14 GP50 Gradient Pump Index 10 Doc 031377 03 7 01 T Technical support 4 1 Temperature A 1 Tests Diagnostic C 21 Keyboard C 20 Pressure transducer C 23 Tilting the front panel 2 2 Time Pump seals backlight in use C 16 Setting the clock 3 12 Time field Method screen C 6 Time Function In screen C 13 D 3 Time based steps entering 3 7 Transducer 2 15 Troubleshooting 4 1 Chromatographic reproducibility 4 12 Degas vacuum pump calibration 4 10 Leak alarm 4 6 Liquid leaks 4 6 Noisy pump motor 4 8 Pump head pressure fluctuations 4 1 Pump out of prime 4 1 Pump stops 4 3 Pump will not start 4 3 Relay control functions 4 11 TTL in
125. reen displays instead of the MAIN screen See Section C 2 7 if this occurs NOTE The POWER UP screen can be viewed at any time by selecting the screen from the DIAGNOSTIC MENU see Section C 2 1 The MAIN screen displays status information in enlarged characters to allow viewing from a distance Use the MAIN screen to select operating parameters such as the flow rate and the percentages of eluents to run To access other GP50 screens press the Menu button to display the MENU of SCREENS see Figure 2 5 Figure 2 3 Power Up Screen Figure 2 4 Main Screen Help Message GP50 GRADIENT PUMP PUMP HEAD VOLUME 100 uL MODULEWARE REV n nn BIOS REV n nn Help Message PSI LOAD METHOD 100 0 A 0 0 B 0 0 C 0 0 D COLUMN A 2000 mL MIN 2 00 LOCAL 2 MIN 10 27 GP50 Gradient Pump 2 8 Doc 031377 03 7 01 There are two ways to select a screen from a menu Press the numeric button on the front panel keypad that corresponds to the screen number on the menu For example press 3 to display the METHOD screen Move the cursor to the field containing the screen number and press Enter See Appendix C for a description of each screen 2 2 Electronics Chassis The electronics chassis is located behind the upper door of the pump enclosure The chassis includes several electronics cards printed circuit boards that are used to control the pump Connectors on the cards also allow communication between the pu
126. s located on the electronics chassis behind the upper door see Figure D 1 Each 2 pin connector includes a signal pin and a ground pin 1 Locate the twisted pairs of wires P N 043598 and two pin connector plugs P N 921019 provided in the GP50 Ship Kit Attach a two pin plug to each end of the twisted pair of wires to be connected The signal wire goes on top and the ground wire goes on the bottom of each plug 2 Connect these plugs to the TTL or relay connectors on the GP50 and the other instruments s as needed for your application Check the polarity of each connection Be sure to connect signal wires to signal pins and ground wires to ground pins If necessary remove wires from the two pin plugs and reinsert them in the correct positions Section D 3 1 shows example connections 3 Route the wires from the GP50 electronics chassis through the upper chase see Figure B 1 to the rear panel GP50 Gradient Pump D 6 Doc 031377 03 7 01 D 3 1 Example Connections Figure D 5 shows an example of TTL relay connections for a Dionex system connected to an AS40 Automated Sampler Refer to the AS40 operator s manual for details Figure D 5 Example TTL and Relay Connections AS40 READY OUT AS40 RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN 1 2 3 4 5 RELAY CONTROL GND F2
127. s no eluent 1 Refill the eluent reservoirs Also make sure that each eluent line extends to the bottom of the reservoir 2 Reprime the pump see Section B 2 8 Pump out of prime eluents are improperly degassed 1 If the GP50 is not equipped with the optional vacuum degas pump assembly degas the eluents manually see Section 3 1 1 Reprime the pump see Section B 2 8 2 If the GP50 is equipped with the optional vacuum degas pump assembly test the degas pump a Open the DEGAS STATUS screen press Menu 8 8 and 3 Press a Select button to toggle the TEST field to RUN and press Enter b The degas pump should turn on and run for about 2 minutes If it does not run see Section 4 6 Pump out of prime end line filter is dirty or clogged 1 Replace the filter P N 045987 2 Reprime the pump see Section B 2 8 GP50 Gradient Pump 4 2 Doc 031377 03 7 01 Pump out of prime blockages in inlet tubing Kinked or clogged tubing causes the pump to be starved for eluent Replace the tubing and fittings Reprime the pump see Section B 2 8 If priming the pump does not eliminate excessive pressure fluctuations the piston seals or check valves may be dirty or defective 1 Follow these steps to isolate the cause a If leaks are seen from the piston rinse tubes replace the piston seals see Section 5 2 b If no leaks are seen replace the check valves see Section 5 1 Impurities in the elu
128. sponds to the screen number on the menu For example press 3 to display the METHOD screen Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Figure C 2 Menu of Screens Help Message MAIN SCREEN DETAIL SCREEN METHOD DEGAS OPTIONS MODULE SET UP PUMP OPTIONS TIME FUNCTION IN DIAGNOSTIC MENU 1 2 3 4 5 6 7 8 MENU of SCREENS C Display Screens Doc 031377 03 7 01 C 3 C 1 2 Main Screen The MAIN screen displays automatically after power up Use the MAIN screen to Select the operating mode and control mode Select basic operating parameters for Direct control mode or display the status of operating parameters when a Method is running Select the method number to run Figure C 3 Main Screen A B C D Displays the percentage of each eluent currently in use When using Direct control set the percentage in these fields These fields will be programmed in the METHOD screen when using Method control LOAD INJECT Selects the position of the injection valve The choices are INJECT and LOAD PSI MPa BAR Displays the the system backpressure in psi MPa or bar Set the unit of measure on the PUMP OPTIONS screen see Section C 1 8 COLUMN Selects the active column A or B in the chromatography module Column B is available only when a column switching valve is installed mL MIN Displays the eluent f
129. such as an integrator autosampler or another Dionex instrument After connecting the TTL and Relay outputs see Section D 3 toggle the output states on and off from the DETAIL screen see Section C 1 3 for Direct control or program the output states from the METHOD extension screen see Section C 1 5 for Method control To turn on a TTL or relay output set the corresponding output field in the DETAIL screen or METHOD extension screen to 1 closed To turn off a TTL or relay output set the corresponding output field to 0 open For example if TTL2 is connected to the Load relay on the AS40 Autosampler setting TTL2 to 1 as shown in Figure D 2 sends the signal to the AS40 to start the load cycle NOTE If the GP50 is connected to a PeakNet workstation PeakNet software can control the TTL and Relay outputs See the PeakNet online Help or user s guide for details Figure D 2 Detail Screen Help Message CURVE 5 2 00 2125 10 25 MIN REMOTE COLUMN 05 DETAIL SCREEN 100 0 A TTL1 TTL2 RLY1 RLY2 0 0 1 1 INJECT 0 5000 PSI 0 0 B 0 0 C 0 0 D LIMIT METHOD PSI mL MIN SAMPLE A D TTL and Relay Control Doc 031377 03 7 01 D 3 D 2 TTL Input Operation The four TTL inputs can be connected to devices capable of providing TTL output signals Each TTL input controls a specific function in the GP50 A signal from the connected device triggers the function associated with that inp
130. sure unit from the PUMP OPTIONS screen see Section C 1 8 P POINT LEFT RIGHT Displays the pressurization points for the left and right pump pistons WEIGHT OF THE WATER After following the instructions for calibration enter the measured weight of the water pumped into the beaker Help Message PUMP DI WATER AT 2000 300 PSI CALIBRATE FLOW CALIBRATION RDY P POINT LEFT PRESSURE RIGHT PSI nnnn nnnn nnnn ALLOW PUMP TO STABILIZE COLLECT WATER INTO A TARED BEAKER FOR 5 MINUTES ENTER THE W EIGHT OF THE WATER 5 00 g GP50 Gradient Pump C 30 Doc 031377 03 7 01 C 3 6 Pressure Calibration Use the PRESSURE CALIBRATION screen to calibrate the pump pressure offset and slope Calibrating the Pump Pressure Offset 1 Stop the pump flow 2 Go to the PRESSURE CALIBRATION screen 3 Open the pressure transducer waste valve by turning it counterclockwise two turns see Figure B 5 Figure C 25 Pressure Calibration Screen Initial View CAL MODE Selects the calibration mode READY The pump is ready for calibration OFFSET CAL Calibrate the offset SLOPE CAL Calibrate the slope SLOPE DFLT Use the default slope value EDIT Enter SLOPE and OFFSET values directly without performing the calibration CAL VALUE Pressure value entered during the slope calibration procedure PRESSURE Current pressure reading from the transducer SLOPE Calibrated slope value PSI MPa OFFSET Calibr
131. tatement in the Dionex Terms and Conditions for more information GP50 Gradient Pump 5 2 Doc 031377 03 7 01 Figure 5 1 Pump Heads and Liquid Lines PEEK Figure 5 2 Pump Heads and Liquid Lines SST Pressure Transducer Outlet Check Valve Pump Head To Column To Waste Inlet Check Valve Pressure Transducer To Column Pump Head To Waste To Eluent Manifold Inlet Check Valve Outlet Check Valve 5 Service Doc 031377 03 7 01 5 3 5 Rinse each check valve housing and cartridge thoroughly with filtered deionized water 6 The inlet check valve assembly housing has a 1 4 28 port PEEK pumps or a 10 32 port stainless steel pumps Replace the cartridge in the inlet check valve housing making sure the double hole end of the cartridge is visible The outlet check valve assembly housing has a 10 32 port PEEK and stainless steel pumps Replace the cartridge in the outlet check valve housing making sure the single hole end of the cartridge is visible Liquid flows through the check valve in the large single hole and out the small double holes NOTE The pump will not operate properly unless the cartridge is installed in the housing in the correct orientation 7 Reinstall the check valves Be sure to install the inlet check valve on the bottom of the head and the outlet check valve on the top of the head Tighten only enough to seat 25 in lb torque Tighten a little more only if it
132. the piston seal and the rinse seal see Section 5 2 2 Check all connections between the eluent reservoirs and the pump heads Tighten the fitting connections just enough to stop the leak Proportioning valve leaks Tighten loose fittings If there are no loose fittings replace the valve see Section 5 5 Pressure transducer leaks Inspect the pressure transducer If the waste valve is the source of the leak replace the waste valve O ring see Section 5 4 If the leak is from the rear of the transducer contact Dionex Technical Support Priming valve Tighten any leaking fittings just enough to stop the leak If this does not stop the leak replace the fittings and or tubing making the connection If this does not stop the leak replace the priming block assembly P N 054086 Contact Dionex Technical Support Overtightening the fitting connections may strip the threads in the valve block If this happens replace the entire manifold assembly P N 046203 GP50 Gradient Pump 4 8 Doc 031377 03 7 01 Interior mechanical chassis leaks Inspect the chassis for leaks Tighten any leaking fittings Replace any damaged parts 4 5 Noisy Pump Motor DSP digital signal processing card current limit has been exceeded The card includes a built in current limiter to protect the motor and motor drive Check the three small LEDs in the upper left corner of the DSP card bulkhead which is in the electronics chassis behind the p
133. time 6 minutes At 7 minutes begin a 3 minute concave segment reducing eluent B to 0 and increasing eluent A to 100 total elapsed time 10 minutes 1 Go to the METHOD screen and enter a method number in the EDIT field 3 for example If Method 3 currently exists and you want to retain it enter a new unused method number in the EDIT field If Method 3 currently exists and you want to delete it move the cursor to TIME INIT and press Delete twice 2 Move to the FLOW field of the INIT step and enter a flow rate of 1 50 Figure 3 13 E1 and E2 Curves 3 Operation and Maintenance Doc 031377 03 7 01 3 21 Figure 3 14 illustrates the METHOD screen as it appears so far You can now begin entering the method steps that will generate the curved gradient profile see Figure 3 13 3 Move the cursor to the A field of the TIME 0 step and press Enter 100 of eluent A is automatically filled in 4 Move the cursor down to the next line and enter 1 in the TIME field and then move to the A field and enter 100 This marks the end of the isocratic section of the run and the beginning of the eluent B concentration ramp At this point the concentration of eluent A begins to decrease from 100 as the concentration of eluent B begins increasing from 0 5 Move the cursor down to the next line and enter 3 5 in the TIME field Move the cursor to A and enter 68 Move the cursor to B and enter 32 6 Move the
134. tronics chassis see Figure 2 6 and in the mechanical chassis are properly connected To check the mechanical chassis connections a Turn off the GP50 power b Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door Pull the drawer out a few inches c Locate the distribution card on the top of the mechanical chassis Check that all cables are seated correctly in the connectors on the card d If the connections are correct the distribution card may need to be replaced Call Dionex Technical Support e Push the mechanical chassis drawer back in place making sure the cables are not pinched Retighten the drawer lock GP50 Gradient Pump 4 10 Doc 031377 03 7 01 4 7 Vacuum Degas Pump Calibration Fails At the end of the degas calibration the DEGAS READING value is less than 13000 counts and one of the following error messages appears Verify that the cable to the vacuum degas pump is connected to the distribution card in the mechanical chassis 1 Turn off the GP50 power 2 Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 Pull out the drawer a few inches 3 The distribution card is on the top of the mechanical chassis Labels
135. ts The assembly which must be installed at the factory consists of A 4 channel degas chamber with degas membranes with 17 mL internal capacity per channel A dual stage diaphragm vacuum pump A solenoid valve An on board vacuum sensor The electronics required to operate the vacuum pump Fittings tubing and other accessories By default the degas pump turns on for 2 minutes when the GP50 power is turned on Thereafter the pump turns on for 30 seconds at 10 minute intervals The DEGAS OPTIONS screen allows you to change the cycle time and duration see Section C 1 6 You can check the vacuum chamber pressure from the DEGAS STATUS screen see Section C 3 4 NOTE All components of the vacuum degas assembly are made of inert materials or corrosion resistant materials However Dionex recommends thoroughly flushing any chemicals out of the tubing with deionized water after each use to avoid crystallization in the membrane pores 2 6 Piston Seal Wash Optional When using highly concentrated buffer solutions Dionex recommends continuously rinsing the piston seal with a piston seal wash Rinsing removes salt crystals that may abrade the piston thereby causing the seal to wear out prematurely and allow leaks Dionex offers a continuous seal wash kit P N 059187 for the GP50 For installation instructions see Section B 2 8 or the instructions included with the kit 2 Description Doc 031377 03 7 01 2 17 2 7 El
136. uent Reservoirs NOTE Dionex strongly recommends degassing all eluents and storing them in reservoirs pressurized with helium This helps prevent bubbles resulting from eluent outgassing from forming in the eluent proportioning valves pump heads and the detector cell Degassed eluents and pressurized reservoirs are especially important when combining aqueous and non aqueous components e g water and methanol Pressurizable reservoirs allow eluents to be stored under a specific atmosphere The following pressurizable reservoirs are available from Dionex 1 liter glass reservoirs with shatterproof plastic coating P N 044126 2 liter glass reservoirs with shatterproof plastic coating P N 044127 1 liter plastic reservoirs P N 044128 2 liter plastic reservoirs P N 044129 Refer to the Pressurizable Reservoir Installation Instructions Document No 034581 for installation details EO1 Eluent Organizer Optional The Dionex EO1 Eluent Organizer P N 044125 holds eluent reservoirs in a liner that contains spills and leaks The EO1 can also be used to pressurize reservoirs Up to two optional E01 Eluent Organizers can be places on top of the system Do not use the 2 liter plastic reservoir P N 044129 for off line vacuum degassing of eluents The reservoir was not designed for this purpose N utilisez pas le r servoir en plastique de 2 litres N P 044129 pour le d gazage vide hors ligne d luants Le r servoir n
137. ump Interface Card NOTE If the DX LAN option was not installed at the factory order the pump interface card kit P N 044195 The kit includes the components required for DX LAN communication STATIC The GP50 electronics cannot be serviced by the user The pump interface card must be installed by qualified personnel Standard anti static procedures must be observed when installing the interface card or handling the CPU card GP50 Gradient Pump B 10 Doc 031377 03 7 01 1 To replace an existing pump interface card remove the hex nut securing the BNC DX LAN connector to the GP50 rear panel see Figure B 1 2 Remove any TTL Relay plugs from the connectors at slot 4 of the electronics chassis see Figure B 2 3 Disconnect the 60 pin ribbon cable from the inside of the front panel by opening the ejector latches on the connector 4 Using a screwdriver as a lever open the white ejector latch at the bottom of the CPU card Remove the CPU card cable and Relay card as a single unit 5 Remove the pump interface card if present from slot 4 of the electronics chassis 6 Insert the new pump interface card P N 046341 into slot 4 a Slide the card to the rear b Verify that the BNC connector is aligned with the opening at the rear and that the card is aligned with the card connector c Press firmly on the card until it mates fully with the connector on the rear panel Pull on the card to verify that it cann
138. ump upper door If the LEDs are flashing in time with the pump strokes the current limiter is being activated As the pump motor ages it becomes less efficient and the current limit is activated more frequently Activating the current limit is harmless but if it occurs frequently even at low speeds and or pressures the bottom plate assembly P N 045670 needs to be replaced Call Dionex Technical Support for assistance Pressure servo oscillation Check the DSP STATUS screen press Menu 8 8 and 3 to verify that the correct pump head volume and head material are selected If the settings are correct but the problem persists notify Dionex Out of prime Reprime the pump see Section B 2 8 4 Troubleshooting Doc 031377 03 7 01 4 9 4 6 Vacuum Degas Pump Does Not Run DEGAS OPTIONS screen settings incorrect Go to the DEGAS OPTIONS screen press Menu and 4 If the DEGAS PUMP field is set to ALWAYS OFF select BY SETTING and then enter the cycle duration and frequency times see Section C 1 6 Electrical cables improperly installed Follow the steps below to manually test the degas pump 1 Go to the DEGAS STATUS screen press Menu 8 8 and 3 Press a Select button to toggle the TEST field to RUN and press Enter 2 The pump should turn on and run for the cycle duration time specified in the DEGAS OPTIONS screen 2 minutes by default If it does not run verify that the cables connected to the pump in the elec
139. us rinse through the system until you resume normal operation Select a flow rate of 0 04 mL min for standard bore pump heads or 0 01 mL min for microbore pump heads and set all four valves in the eluent manifold to 25 so that the valves are also flushed Before a shutdown of more than three days reduce the pressure on the eluent reservoir s to approximately 21 KPa 3 psi Shut down the pump by turning off the main power on the GP50 Flushing the eluent manifold is extremely important if your eluents have a combination of salt or base and solvent If salt precipitates in the valves the valve diaphragms may be seriously damaged If this happens you will have to replace the entire valve assembly GP50 Gradient Pump 3 28 Doc 031377 03 7 01 Doc 031377 03 7 01 4 1 4 Troubleshooting This chapter is a guide to troubleshooting minor problems that may occur while operating the GP50 Gradient Pump To use this guide turn to the section that best describes the operating problem There possible causes of the problem are listed in order of probability If you are unable to resolve a problem contact Dionex Technical Support In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex office 4 1 Left Right Pump Head Pressure Fluctuations The GP50 display updates the pressure readout once per piston stroke A variation of more than 3 from one stroke to the next indicates a problem Pump out of prime there i
140. ut TTL input 1 turns the pump motor off and on TTL input 2 controls the pump method clock A signal from the controlling device can turn the method clock on Run or off Hold Reset Hold Reset resets the method clock to zero and executes INIT conditions The method will not run unless the pump motor is already on TTL input 3 increases the method number by one Each additional signal increases the method number by one more When the method clock is zero or INIT increasing the method number executes the INIT conditions of the new method When the method clock is greater than zero increasing the method number begins running the new method at the current elapsed method clock time This has the same result as selecting a new method number when the pump is in Local mode TTL input 4 decreases the method number by one The operation is as described under TTL input 3 above except that the method number decreases D 2 1 TTL Input Signal Modes The GP50 TTL inputs respond to four different types of device output signals The default signal mode normal edge is compatible with the output signals provided by Dionex modules If the device connected to the GP50 outputs a different signal type select the appropriate signal mode from the TIME FUNCTION IN screen see Figure D 3 Figure D 3 Time Function In Screen Help Message HOLD RESET INIT COND RUN INCREMENT METHOD NUMBER 1 2 3 4 TIME FUNCTION IN PUMP MOTOR OFF ON M
141. y and EN 55011 1991 emissions The symbols below appear on the GP50 or on GP50 labels Alternating current Protective conductor terminal Power supply is on Power supply is off Doc 031377 03 7 01 2 1 2 Description The GP50 Gradient Pump consists of two units see Figure 2 1 The upper unit houses the electronics components and the lower unit houses the pump heads and other mechanical pump assemblies Figure 2 1 GP50 Enclosure Pump Heads GP50 Gradient Pump 2 2 Doc 031377 03 7 01 2 1 Front Control Panel The control panel on the upper door of the pump enclosure contains a liquid crystal display LCD a membrane keypad and the actuator for the main power switch see Figure 2 2 The door opens to provide access to the electronics chassis see Section 2 2 NOTE If no keypad buttons are pressed within a two hour period the front panel backlight will automatically turn off To restore the backlight press any button Screen Contrast Information is displayed on the LCD also called the screen To adjust the screen contrast use the knurled knob in the recess below the keypad see Figure 2 2 Tilt Panel To maximize visibility the front control panel can be tilted to four different positions To tilt the panel support the door at the left side to prevent it from opening and lift firmly on the tab in the middle of the recess below the keypad see Figure 2 2 Push on the tab to return the pane
142. yboard Test Screen Help Message OFF ON PRIME INSERT DELETE RUN HOLD RESET SEL UP SEL DOW N UP 7 8 9 4 5 6 1 2 3 0 E DOWN LEFT RIGHT HELP MENU C Display Screens Doc 031377 03 7 01 C 21 C 2 7 Diagnostic Test Use the DIAGNOSTIC TEST screen to test the GP50 electronics At power up some of these tests are run automatically If any tests fail additional tests may be run after the DIAGNOSTIC TEST screen is displayed If errors occur a message screen displays When you clear the message the DIAGNOSTIC TEST screen reappears It remains open until you press the Menu button Figure C 17 Diagnostic Test Screen PROPORTION ING VALVE Selects a valve and displays its status OPEN or CLOSED When the screen opens valve A is selected and the status field is blank To test a valve first turn off the pump motor Then press Select or Select to select the desired valve and press Enter The valve opens Thereafter pressing Enter toggles the valve closed and open Pressing Delete followed by Enter also closes the valve Exiting the DIAGNOSTIC TEST screen when the pump motor is off closes all valves Help Message PROPORTIONING VALVE DIAGNOSTIC TEST A POWER CPU DX LAN P LOOP COM DSP COM OVEN COM PUMP LEAK OPEN GP50 Gradient Pump C 22 Doc 031377 03 7 01 To manually run a test position the cursor in the edit field n

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