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Leica RM2255 - Research Programs & Labs

1. Insert a small screwdriver into the cutout 25 and carefully pry out the insert Remove the voltage selector housing 21 together with the fuses 23 Remove the fuses While doing so leave the voltage selector switch block 24 in the housing Replace defective fuses and reinstall the voltage selector into the instrument Check that the correct voltage value Is displayed in the viewing window 22 69 8 Cleaning and maintenance 8 2 2 Maintenance instructions Only authorized and qualified service personnel may access the internal components of the instrument for service and repair The instrument Is basically maintenance free To ensure trouble free operation of the instrument over a long period of time the following Is recommended by Leica 1 Thoroughly clean the instrument on a daily 3 Have the instrument inspected on a regular basis basis by a qualified service technician au 2 Enter into a service contract at the latest at thorized by Leica the end of the warranty period For more The intervals depend on how heavily the information please contact your local Leica instrument is used technical service center We recommend the following inspection intervals depending on the general workload of the instru ment as defined in the two categories listed in the table Category Category Il Number of sections per day gt 8 000 sections per day lt 8 000 sections per day Workloa
2. Unpack all accessories in the package and check for completeness Baseplate with drilling a Vertical column with size 8 cap screw b and lock washer c Horizontal arm with cross member d and support ring e Support plate large for RM2235 RM2245 and RM2255 Support plate small for RM2265 Allen key size 3 4 countersunk screws to install support plate Allen key size 8 Assembling the universal microscope carrier Attach baseplate Select the large 5 or small 4 support plate depending on the microtome to be used Attach the support plate to the baseplate with the included 4 countersunk screws 7 using the Allen key size 3 6 Instruction manual V 1 4 02 2007 6 Optional accessories Leica RM2255 Attach vertical column Insert the cap screw b into the hole of the baseplate from below Place the lock washer c on the cap screw from above Thread the silver vertical column 2 onto the baseplate from above and tighten with the Allen key size 8 The lock washer must be positioned between the baseplate and vertical column to prevent unintentional turn ing of the column Extreme danger of tipping After install ing the vertical column immediately place the microtome onto the baseplate so that the front feet of the microtome are positioned in the shallow recesses A Attach horizontal arm Slide the support ring e onto the vertical column and position it so that the lo
3. CE Instruction Manual Leica RM 2255 V1 4 English 02 2007 Always keep this manual with the instrument Read carefully before working with the instrument Leica RM2255 Rotary Microtome Seica MICROSYSTEMS NOTE The information numerical data notes and val ue judgments contained in this manual repre sent the current state of scientific knowledge and state of the art technology as we under stand it following thorough investigation in this field We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical develop ments nor to provide our customers with addi tional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case In particular no liability what soever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other informa tion in this manual Statements drawings illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products Published by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 0 6224 143 0 Fax 49 0 6224 143 200 In
4. instrument for service and repair e Before each cleaning switch off the instrument disconnect the power plug remove the knife holder completely and clean it separately Always remove the blade before detaching the knife holder from the instrument Lock the handwheel before each cleaning Do not use any solvents containing acetone or xylene for cleaning Ensure that no liquids enter the interior of the instrument when cleaning Do not turn the instrument on before it is completely dry When using detergents please comply with the safety precautions of the manufacturer Turn the instrument off with the mains switch and pull the mains plug before replacing the fuses Only use fuses of the same specification For fuse specifications refer to chapter 3 3 Technical data A e Only authorized and qualified service personnel may access the internal components of the Leica RM2255 9 2 Safety 2 3 Integrated safety devices 10 Fig 3 Emergency stop function The emergency stop function is activated with the red EMERGENCY STOP switch 1 on the upper left of the front of the microtome The sectioning motor stops immediately when the EMERGENCY STOP switch is pressed The red LED in the E STOP field 2 on the control panel of the in strument lights up indicating that the emergency stop func tion has been activated To deactivate this function turn the EMERGENCY STOP switch in
5. 7 2 Possible faults Problem Possible cause Corrective action 1 Thick thin sections The sections alternate between being thick and thin In extreme cases there are no sections whatsoever 2 Section compression The sections are very com pressed show folds or are squeezed together 3 Stripes in sections For knife holder E 4 Noises during sectioning The knife sings when sectioning hard specimens The sections show scratches or chatter marks 5 High blade consumption 66 e Insufficient clamping of blade knife holder specimen or orien tation e The blade is dull e The pressure plate is damaged or Incorrectly adjusted e Clearance angle of the knife blade too small e The blade is dull e The specimen is too warm e The sectioning speed is too fast e There is an accumulation of paraffin on the rear pressure plate of the knife holder e The sectioning speed is too fast e The clearance angle Is too wide e Insufficient clamping of speci men clamp and or knife holder e Too great of a sectioning force was applied Check the clamping positions and repeat the clamping process Laterally displace the knife holder or insert a new blade Insert a new pressure plate or use a new knife holder Methodically experiment with larger clearance angle settings until you have found the optimum angle Use another area of the blade or a new blade Cool the specimen before sect
6. ssssssssrsrsrsrsrsrurerrnunurnririririniisinrnrsrsrsrernnns 0212 30350 1 operating manual G E F S asses cnsnrerottaanedacaiensetieaeecnmernccaaasn 0700 37112 i The accessories are included at the top of the package item 2 in Fig 9 Check the delivery carefully against the packing list delivery note and your order Should there be any discrepancy please contact the Leica selling unit handling your order Instruction manual V 1 4 02 2007 4 Startup 4 3 Unpacking and installation Fig 9 Leica RM2255 Unpacking Do not transport the instrument by holding it on the handwheel shaft the Loosen and unscrew the four upper screws 7 Remove the cover 1 Take out the accessories box 2 and instruction manual Take out the fixing module 3 Remove the eight lower screws 8 Carefully pull up the wooden case A object head or the cover Lift the instrument 5 by holding it by the base plate and the recessed grip on the back see Fig 10 and lift it out of the formed cushion 6 Fig 10 Setting up the instrument Place the instrument on a stable laboratory table The two sliding elements 9 located on the rear of the base plate make It easier to move the instrument on the table To move the instrument hold it by the front of the base plate lift it up gently and slide it on its slides 17 4 Startup 4 4 Assembling the ha
7. B FBLE printed circuit boards HAS 7028 electronic components LRP PE mechanical parts optical ce Spe ee components eames S TT OT OT a iightsouces 9 light sources o RAUBER SYORAAUMTPRARBASIT 11363 2006 AEA ENRE ERAF Indicates that the concentration of the hazardous substance in all materials In the parts is below the relevant threshold of the SJ T 11363 2006 standard xX RABABASDAELECAFNR HE PHS BRUSIT 11363 2006 IERTARE RR Indicates that the concentration of the hazardous substance of at least one of all materials In the pars is above the relevant threshold of the SJ T 11363 2006 standard Note The actual product may or may not include in all the part types listed above 74 Instruction manual V 1 4 02 2007
8. 21 Salley OLE eanna a EE NEN a 1 De N A A A E A E 1 2 3 Integrated safety devices cccccessessecessessssessesrsessecessessnseecessesseseesesseseeseusessesensessesensansensesensensas 10 Instrument components and specifications c ccccccecessessesescsseeseesessesesseeeseeeseneeeens 12 3 1 Overview instrument COMPONENTS cccecceesecsseessssssssserssessncessesensescesensensessessnseusessesensensasensensas 12 3 2 Instrument specifications serciacssctusgnistniessnsdnacediudetoinsioindtagioniatatageahssaradnsiusbndagd iedetanatistatdbeimeaniataaeersins 13 IS Tecnica da a n A E E A E E 14 S E E E e eet cee aa tce ences 16 4 1 Installation site requirements saciaccsisiisscsinnsisvoncantisevarwacstadevaniuby dovennadQeasantnstinsshanctentnontiedaveniaunertaeanatis 16 42 Standard deliVely sssrin EE EEEE ENE E EEE 16 43 Unpack ogandinstalatioN ssrssosriesen enren iaa En aR ESR 17 4 4 Assembling the handwheel ccccccssccsssssecsssrsssssesessessesescesesensessessnseesesessuseesesseseesensarenseneas 18 Ao EVE NIG al CONC CONS i S E E 18 4 6 Switching on the instrument aeess sscearsmcuaartenadcatenueoeadectaanisaunesnives enetaiyeigtaatinninsecrantontvetuumeguicstaeiaate 21 i E E ENEE ee ee eee 22 5 1 Operating elements and their FUNCTIONS ccceccessesscsserssecsesessessesessessesencessesensessesenseesenersnseneas 22 5 11 Mstumentcontrol DATEL artesacussnnnsangenannonseasancemrnetusiterostenndisaveraiaioeennc
9. After starting sectioning a single sectioning stroke is completed The specimen then stops automatically in the upper end position of the vertical stroke In motorized sectioning mode the sectioning process can be started and stopped with the foot switch optional accessory instead of the RUN STOP and ENABLE buttons See Foot switches p 32 STEP step stroke operating mode e Select STEP step stroke operating mode After starting the sectioning process the specimen is moved as long as the keys are held depressed or as long as the foot pedal is pressed lf the buttons or the foot switch are released the specimen stops automatically Instruction manual V 1 4 02 2007 5 Operation Indication of remaining horizontal feed Object head ae HOVE Remaining horizon tal feed 1 mm Total horizontal feed Fig 19 automatically The visible and audible remaining feed indica tion feature informs the user during trimming and sectioning when a remaining feed of approximately 1 mm is available before the front limit is reached The yellow LED 41 in the COARSE FEED button lights up from the beginning of the remaining feed In addition an acoustic signal is heard for approx 2 seconds The sectioning process Is interrupted and the object head stops in the upper end position From this point on a remaining feed of approx 1 mm is available In the remaining feed area no mo
10. The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the own er of the instrument and in addition the designated personnel who operates services or cleans the instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings Leica RM2255 2 Safety Warnings Safety instructions warning labels attached to the instrument correct operating instructions as described in this manual must be followed when operating or replacing the instrument component bearing the label Failure to adhere to these instructions may result in an accident personal injury damage to the instrument or accessory equipment A e Safety instruction labels on the instrument marked with a warning triangle indicate that the Warnings Transport and installation e Never lift the instrument by the handwheels or the cassette clamp Always remove the section waste tray before transporting the instrument e Caution The voltage selector has been preset at the factory Before connecting the instrument to the mains power please check that this setting complies with the local power requirements of your laboratory The mains cable inlet is closed with an adhesive tape that indicates the actual voltage setting of the instrument upon arrival Severe damag
11. turn counterclockwise e Continue this procedure until the object head no longer moves once released Instruction manual V 1 4 02 2007 5 Operation 5 2 Inserting the knife holder 55 53 Enlarged detail Scale for better repositioning of the knife holder for varying specimen heights 58 Fig 24 Leica RM2255 Setting up the knife holder base e Release the clamping lever 50 by rotating it counterclockwise e Insert the knife holder base 51 using the notch 52 on the bottom into the T piece 55 of the microtome base plate 53 e To secure the knife holder base turn the clamping lever 50 clockwise The knife holder base 51 can be moved back and forth on the microtome base plate This allows bringing the knife holder to optimal sectioning position in relation to the specimen There is a scale 54 on the right side of the microtome base plate This enables faster and better positioning of the knife holder at the specimen if various combinations of standard Specimens and specimen holders are used The rear edge of the knife holder base 51 functions as the scale reference Inserting the knife holder e Loosen the screw 58 using an Allen key size 4 71 until the knife holder 57 can be moved e Place the knife holder 57 with the under side groove onto the T piece 56 of the knife holder base 51 e To clamp retighten the screw 58 35 5
12. 02 2007 6 Optional accessories Leica RM2255 Fig 30 6 1 4 Quick clamping system lt is used as specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators or the directional fix ture for specimen clamps e Screw the 4 screws 13 into bore A with an Allen key size 2 5 and tighten them 43 6 Optional accessories 6 2 Specimen clamps and holders non directional specimen holder fixture i All specimen clamps available as accessories can be integrated into either the directional or 67 Fig 31 Fig 32 A4 6 2 1 Standard specimen clamp The standard specimen clamps are available in two sizes 40 x 40 mm and 50 x 55 mm They are designed for direct clamping of rect angular blocks In addition they accommodate the foil clamps e Turn the knurled screw 66 counterclock wise to move the movable jaw 68 down ward e Mountthe sample 67 as required e Turn the knurled screw 66 clockwise to move the movable jaw upward against the fixed jaw to securely clamp the sample 6 2 2 Vee insert The vee insert 70 is mounted in the hole pro vided in the lower movable jaw of the standard specimen clamp This makes it possible to clamp round speci mens in the standard specimen clamp e Turn the knurled screw 66 counterclock wise to move the movable jaw 68 down ward e Insert the
13. 8 Cleaning and maintenance ccc cescccescsscseeecsececsecsececsesseecsesaesesensasessesasesensatseseneaees 67 8 1 Cleaning the instrument snsisceacctaeevannanantansonssnastacantearhiyacer ss iuinsaneaetamcantcasmvanicdansnivutndestagertumcimendtvedyiencst 67 82 Maintenance soriirienianiainiusnen nsi eea stata fades iann e eie a aa araia 69 eZ ol FC bel CPN CIS OS aE E AT A EE E E EN 69 8 2 2 Maintenance instructions scdsscnssstccinestacheiss socaadscinotasaviasndnnseanteinstantestninatiieabinidinasrtnandesmansimeianenlts 70 8 2 9 Lubricating the instrument scssccstossocmasisinniasainnmian oes iniitersnnnireaitnatancdat saan Yatesseonsautavssangdunmcaabagioteamenadst 71 9 Warranty and service ooo ccccsccecesceeececceeceeceeseeseeseeseeseeseessesessesseuseaseuseusensensensensenseess 72 10 EC Declaration of Conformity 0 0 0 0 ccc cece ccececeecsscecsecseseesesecsessesassesenseeseseusesseseusesensaaees 73 People s Republik of China ju ccc ecsssssssssessessessssssssssessessssssssssessessssessessessnsasenssessesassaseesansess 74 Leica RM2255 5 Important notes 1 1 Symbols in the text and their meanings Dangers warnings and cautions appear in a gray box and are marked by a warning triangle A appear in a gray box and are marked i Notes i e important user information by an information symbol i Numbers in parentheses refer to item 5 numbers in illustrations Function keys which m
14. Allen key size 4 6 1 2 Directional fixture for specimen clamps Loosen the eccentric bolt 6 by turning it counterclockwise Completely unscrew the thrust piece 5 with a flat tip screwdriver and pull it out with spring 5a and pin 5b Completely unscrew the setscrews 3 and 4 Attach the directional fixture for specimen clamps as shown Insert the screws 7 8 in the bore 2 screws 8 are accessible through the bore 9a and evenly screw them In using a 3mm Allen key Insert the spring 5a and pin 5b with the flat ter side into the thrust piece 5 Completely screw in the thrust piece with a flat tip screw driver Completely screw in the setscrews 3 4 41 6 Optional accessories 42 6 1 3 Fine directional fixture for specimen clamps e Before the fine directional fixture for specimen clamps can be mounted loosen 4 screws 10 Allen key size 3 and carefully remove the fixture for specimen clamps from the baseplate 9 e Using the 4 supplied screws 11 and the Allen key size 3 fasten the baseplate to the object head 12 e Now screw the fine directional fixture for specimen clamps with the 4 screws 10 and the Allen key size 3 onto the object head If the fine directional fixture for spec imen clamps is not used retain the baseplate and 4 screws 11 together with the fine directional fixture for specimen clamps Instruction manual V 1 4
15. Problem Possible cause Corrective action e No display no reaction to but 1 The power cable is not connect 1 Check the connection of the tons pressed after the instru ed correctly power cable ment is switched on 2 Power fuses are defective 2 Replace the power fuses see Chapter 8 2 1 3 The cable of the control panelis 3 Check the connection of the not connected correctly cable to the control panel 4 The voltage selection switchis 4 Check the voltage setting and set Incorrectly correct it if necessary see Chapter 4 5 64 Instruction manual V 1 4 02 2007 7 Customized solutions Problem Possible cause Corrective action e The cutting motor cannot be started Fig 65 e Feed motion no longer occurs The cutting motor cannot be started e The coarse feed buttons can be used to move the object ina backwards direction only e The specimen in the residual path area cannot be returned using the coarse feed buttons e Cutting motor switches off prematurely in CONT continu ous stroke operating mode e HOME and STOP are lit up simultaneously Leica RM2255 1 The EMERGENCY STOP button is pressed or the foot switch Is depressed The handwheel lock is activated The motor was temporarily overloaded and was switched off via the automatic fuse The foot switch or dummy plug is not connected or not con nected correctly The end of the residual path has
16. clamp ice cooled Use the ice cooled UCC with the non directional specimen holder fixture only e ce cubes can be made using the included Paraflex mold a e Quick clamping system b see page 43 Fig 37 Leica RM2255 47 6 Optional accessories 6 2 6 Holder for round specimens i The holder for round specimens is designed to accommodate cylindrical samples Inserts for specimens of 6 15 and 25 mm diameter are available 92 89 1 48 Fig 38 To mount the required insert 89 1 3 turn the clamping ring 90 counterclockwise and remove it Place the required insert into the tension ring 90 and screw the tension ring onto the thread 91 by turning it clockwise Mount the sample 67 and fix by turning the clamping ring 90 clockwise To orient the inserted sample insert the pin 92 into the bore 93 and rotate it counter clockwise to release the clamp You can now rotate the specimen so that the side you want faces upwards To lock it in the position you have chosen tighten the pin 92 by turning it clockwise Instruction manual V 1 4 02 2007 6 Optional accessories 6 2 7 Super mega cassette clamp Fi 39 Assembly of the super mega cassette clamp The super mega cassette clamp should preferably be used with the rigid fixture for specimen clamps To do so proceed as follows Remove the rubbe
17. condensation to form inside the instrument After transporting please wait at least 2 hours to allow the instrument to adopt the ambient temperature before turning it on Failure to comply with this may cause damage to the instrument Leica RM2255 19 4 Startup Fig 13 20 Connecting the dummy plug e Insert the dummy plug provided 18 into the connecting jack 19 on the rear of the in strument and fasten it by tightening the screws 20 Connecting the foot switch optional accessory e lf afoot switch is to be used with the instru ment attach it in the same way as the dummy plug i Caution If neither the dummy plug nor the foot switch is connected the instrument is not ready for operation In this case the E STOP LED on the instrument is illuminated Connecting the control panel The connecting cable 15 of the control panel is fixed to the microtome It may not be discon nected e Insert the plug 14 of the connecting cable 15 into the connecting jack 16 on the rear of the control panel e To fasten the plug tighten the two screws 17 Instruction manual V 1 4 02 2007 4 Startup 4 6 Switching on the instrument When turning the instrument on with the mains switch do not press any of the buttons of the control panel or the foot switch optional accessory A mm Leica RM2255 Turn the instrument on with the mains switc
18. continuous stroke operating mode e Press the foot switch once briefly to start motorized sectioning If the foot switch remains pressed for longer than half a second the specimen stops in the next upper end position e Press the foot switch again to stop it The specimen stops in the next upper end position SINGLE single stroke operating mode e Press the foot switch once briefly to start motorized sectioning After every step the specimen stops automatically in the upper end position STEP step stroke operating mode e Press down the foot switch to start the sectioning process The speci men is now moved for as long as the foot switch is depressed e f the foot switch is released the specimen remains stationary in the position that it has reached How to activate the emergency stop function e Press the foot switch strongly to activate the emergency stop function Sectioning stops immediately The red LED in the E STOP field on the instrument Fig 14 is lit up as long as the foot switch remains depressed e To continue restart the sectioning process using the foot switch The operating mode remains unchanged Instruction manual V 1 4 02 2007 5 Operation Directional fixture for specimen clamps i In the quick clamping device of the directional specimen holder fixture all specimen clamps available as optional accessories can be used implemented The object orientation allows
19. e Sliding surfaces of the finger guard 8 e Knurled nuts 73 for measuring height ad justment Universal cassette clamp e Axle of the clamping lever 90 of the cas sette clamp After lubrication move the lever back and forth several times 71 9 Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual prod uct delivered has been subjected to a comprehensive quality control pro cedure based on the Leica in house testing standards and that the prod uct is faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty Is based on the content of the concluded agreement The warranty terms of your Leica sales organization or the or ganization from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer service or spare parts please contact your Leica representative or the Leica dealer where you purchased the instrument Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Delivery date Decommissioning and disposal The instrument or parts of the instrument must be disposed of according to existing local applicable regulations Special attention should be paid to the lithium cell of the electronic
20. guard on the knife holder Each knife holder is equipped with a tightly mounted knife guard 8 9 This makes it possi ble to cover completely the cutting edge in ev ery knife or blade position Knife holder N NZ The knife guard 8 of the knife holder N NZ can be easily positioned via the two handles 7 Fig 5 To cover the knife edge push both cover strips of the knife guard to the center Knife holder E The knife guard on knife holder E consists of a red foldaway handle To cover the cutting edge fold the knife guard handle 9 upwards as illus trated in Fig 6 A For the new knife holder E the two clamping levers 10 11 must always remain in the position shown Clamping lever for the blade 10 at the right clamping lever for the lateral displacement 11 at the left 11 3 Instrument components and specifications 3 1 Overview instrument components Leica RM2255 Tray Universal Control panel on cassette clamp the instrument Clamping lever for Handwheel lateral displacement locking mechanism Smooth turning Knife holder E handwheel with lateral displacement aro iar activating the Knife holder base handwheel brake Socket for backlighting Emergency stop Clamping lever switch of knife holder base Directional specimen holder Handwheel fixture with quick handle clamping system with centering function Knife guard Separate control panel on the knife
21. holder Section waste tray Fig 7 12 Instruction manual V 1 4 02 2007 3 Instrument components and specifications Rear side of instrument Magnet for holding the wrench Motor Heat sink protection fuse Voltage selector i f Connecting switch with fuses cable for control panel Main switch Power l Connecting jack connection for foot switch Fig 8 3 2 Instrument specifications The Leica RM2255 is a motorized rotary The specimen retraction can be turned off microtome In manual operation the retraction can be e The specimen feed system with zero back adjusted In motorized operation the lash and maintenance free cross roller retraction value varies with the selected guides and the stepper motor operated sectioning speed An LED illuminates while coarse feed system are located in a dust the sample is in retraction proof plastic housing e The electric coarse feed operates at two e The instrument is equipped with a safety speeds In the sectioning mode the coarse handwheel with a handle that can be feed buttons have a STEP function centered as well as a mechanical hand Three motorized sectioning modes CONT wheel lock SINGLE and STEP are available as well as e All controls and LEDs are centralized in a one manual sectioning mode the rocking separate control panel mode ROCK All control elements are logically arranged in In rocking mode it is enough to move the functional groups and ea
22. rigid 51 can be moved forwards and backwards on the microtome base plate This vertical displacement allows bringing the knife holder into the optimal cutting position in relation to the specimen e To release rotate the clamping lever 50 on the right side of the microtome base plate counterclockwise e Reposition the knife holder together with the knife holder base forward or backward as appropriate e Secure the clamping mechanism by rotating the lever 50 clockwise Instruction manual V 1 4 02 2007 6 Optional accessories 6 3 2 Knife holder E E TC The knife holder E TC is designed for the Leica TC 65 tungsten carbide blades Knife holder E TC Fig 42 Prior to inserting the blade both knife holder and knife holder base must have been installed on the instrument Inserting the blades knife holder E and E TC Fold knife guard 9 downward To insert the blade flap the right clamping lever 10 forward and down Carefully insert the blade from the side Make sure that the blade is clamped parallel to the upper edge of the pressure plate To clamp the blade rotate clamping lever 10 back upwards i The knife holder E is designed for conventional disposable blades from all current manufac turers It is available in two models one for narrow band blades and one for broad band blades The knife holder has a lateral movement so that the enti
23. supply voltage specified on the identification label on the underside of the instrument only Connect the power cable 1 to the connect ing jack on the rear of the device Plug the power plug into the power socket Carefully read the instruction manual provided separately before use Fig 64 Leica rotary microtome with installed optional Attach the fiber optic light guide 2 to the adapter of the magnifying lens and secure it in place in the bore 6 using the screw 5 Pull off the protective cap 3 from the end of the fiber optic light guide and keep It in a safe place Insert this end into the opening provided 4 until it engages with a slight click Switch on the Cold light source using the power switch 7 Remove the protective caps 8 and align the light guide with the specimen accessories magnifying lens and fiber optic light guide 61 6 Optional accessories 6 12 Ordering information 62 Fixture for specimen clamps rigid silver sssrin 0502 38160 Fixture for specimen clamps directional silver eessen 0502 38949 Fixture for specimen clamps directional silver sessen 0502 37717 Quick clamping system complete c cc cccsessessssesssssecsseesnseneeees 0502 37718 Knife holder base rigid silver c ccscsssssescssesrssecsesessesesetersenseneeees 0502 37962 Knife holder N RM22xxX silver ccccsessesssesessesessessesessrsnsateesensaneeees 0502 37993 Knife holder NZ RM22xxX s
24. 0 down ward and pull it out sideways Remove pressure plate 83 Clean all parts of the knife holder If several knife holders are cleaned at the same time the parts must not be mixed up do not use xylene or cleaning fluids containing alcohol e g glass cleaner Dry knife holder and reassemble After cleaning the moving parts apply a thin coat of drive part oil to them see also Chapter 8 2 3 When installing the pressure plate 83 en sure that the upper edge is parallel to and level with the rear edge of the knife holder base 86 see also Fig 41 Page 50 Instruction manual V 1 4 02 2007 8 Cleaning and maintenance Universal cassette clamp paraffin residues Fig 68 8 2 Maintenance Fig 69 Leica RM2255 e Detach cassette clamp 13 for a thorough cleaning removing all e For cleaning do not use xylene Use xylene substitutes or paraffin removers such as Para Gard e The cassette clamp 13 can also be placed in an oven heated to a maximum of 65 C until the liquid wax escapes e Remove paraffin residues with a dry cloth e After such a cleaning procedure in an oven always be sure to lubri cate the axle of the clamping lever 60 see also chapter 8 2 3 8 2 1 Replacing fuses Switch the unit off and unplug it Use only the supplied replacement fuses Both fuses must have the same rating check the imprint
25. AR to reset section thickness sum or section number e This will only reset the currently displayed value Caution When the instrument is switched off using the main power switch both values section thickness sum and section number are erased from memory Specimen retraction To prevent damage to the blade and specimen the specimen is moved away from the blade during the return motion to the upper home position In motorized sectioning mode the retraction depends on the setting of the sectioning speed In manual mode the retraction value can be selected in 5 pm Increments between 5 and 100 um Specimen retraction is set to 10 um at the factory The specimen retraction can also be deactivated for the manual and motorized operation if required The selected setting is maintained when the instrument is turned off 26 Instruction manual V 1 4 02 2007 5 Operation Configuring the retraction settings e To call up the retraction settings press the MENU MODE and CLEAR 3 Clear Mode buttons simultaneously The current set value is displayed as a three digit number in the four digit display e g 025 25 um Select the desired retraction value The retraction value can be adjusted in increments of 5 um to a maximum of 100 um using the buttons on the control panel 5 e To exit the retraction settings press MENU MODE A retraction movement takes place by the newly selected value after a
26. Operation 5 3 Inserting the universal cassette clamp Fig 25 36 There are two versions of the specimen holder one with and one without specimen orientation which are interchangeable The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place You can use the specimen holder fixture 60 to hold all available accessory specimen clamps for more information see Chapter 6 Optional accessories To do so proceed as follows e Move the specimen holder fixture 60 to the upper end position by turning the handwheel 12 and engage the handwheel lock e To release the clamping system turn the screw 61 counterclockwise using an Allen key size 4 71 e Push the guide 63 of the specimen clamp 62 from the left into the specimen holder fixture 60 as far as it will go e To clamp the specimen clamp turn the screw 61 clockwise as far as it will go Instruction manual V 1 4 02 2007 5 Operation 5 4 Adjusting the clearance angle e The index marks 0 5 and 10 for adjust ment of the clearance angle 59 1 are located on the right side of the knife holder 57 e There is also an index mark 59 2 on the right side of the knife holder basis 51 which serves as a reference point when adjusting the clearance angle e Loosen the screw 58 using an Allen key size 4 71 until the knife holder 57 ca
27. ach end of the knife support bar Prior to inserting the knife both knife holder and knife holder base must have been installed on the instrument Enlarged detail ew Knife inserted and Ns Knife holder NZ height adjusted 4g For holding conventional and hard metal knives up to 16 cm in length Knife pressure plate 56 for extreme stability and full utilization of the knife blade Knife holder N For holding convention al knives up to 16 cm in length Fig 47 54 Instruction manual V 1 4 02 2007 6 Optional accessories Inserting the knife Knife height adjustment Rotate the knurled nuts 48 on the right and left of the knife holder forward in opposite directions lowering the knife support bar to the lowest possible position thus ensuring that the knife edge will not be damaged when inserting the knife Unscrew the clamping screws 49 as far out as possible rotate coun terclockwise Hold the knife 47 at the knife back and carefully insert it in the holder from the side as shown with the cutting edge facing upward When adjusting the clearance angle the knife edge should be positioned as exactly as possible in the actual center of rotation of the knife holder The lay on edge 39 of the rear clamping chucks serves as a reference position for correct knife height adjustment The knife edge should be parallel with the locating edges Rotate the knurled nuts 48 unifor
28. acle at the bottom of the dispenser or remove the knife from the knife holder and put it back in the knife case e Remove knife holder base and knife holder for cleaning e Remove the specimen from the specimen clamp e Remove section waste with a dry brush e Remove specimen clamp and clean separately Instrument and outside surfaces lf necessary the varnished outside surfaces of the control panels can be cleaned with a mild commercial household cleaner or soap water and then be wiped with a moist cloth To remove paraffin residue xylene substitutes paraffin oil or paraffin removers such as Para Gard Polysciences can be used The instrument must be completely dry before it can be used again Cleaning the knife Always wipe the knife from the back of the knife to the cutting edge NEVER wipe in the opposite direction risk of injury Clean using an alcohol based solution or acetone Leica RM2255 67 8 Cleaning and maintenance Knife holder E Fig 67 68 Take the knife holder apart for cleaning To do so proceed as follows A For cleaning and removal of paraffin Fold the knife guard 9 and ejector 84 downward Rotate the clamping lever 11 of the lateral dis placement forwards and pull it out sideways Push the knife holder base plate 86 with the pressure plate 83 until it can be removed from the segment arch 87 Rotate the blade clamping lever 1
29. anamwieadiesiiusansierdeiarieamtomrscdurstenth 22 912 OMEN OND EVE circa i da aaa 23 5 1 3 Display and control GlOME NICS sacsccsiecessosmmvngeveadunsadesratanaiteaniSacaradbsinsteveaninnsiinaiumiaddnontvnedawianneromien 24 5 2 Inserting the knife holder sssesesseesesrsesisrsrnrururururururununurununununanananunnnnnnnnnrnrnrnnnrnnunnnnnnnananennrarenernrrens 35 5 3 Inserting the universal cassette clamp c cccessscseccsssssssecssersssseeseseessesescessesensessesensensensersnsanees 36 5 4 Adjusting the clearance angle cccccesessesscssesssssssscecsrssessesessesseseesessessusesseseesansessesensaesenesensaneas 37 59 Glamping TIVES 6 CC MTG a ssnin eaa anaanatae 38 5 6 Clamping the knife disposable blade ssessssesessrsrerrnrnunsrrnenininenennnrninrnrsrsrnrnrururnrnrerennrerererrnreens 38 5 7 rimming the Specimen sec seraeeseaqtneverapnsisstnsersitesigusnsivaccueraivaenianmndcancdvenisteeaietnsiastiasateoammieauiinicdudents 39 5 7 1 Trimming in manual operation ccccceseesssecscssesessessecessessnssesesseseeseesesseseusessesensensessesensaesessesenseneas 39 5 7 2 Trimming in motorized operation sciences sno vnscrectnnssaceetaticernansausenionseeeainoningesaiiamedaasidieanmesanvanareentl 39 OF SECURING rosier E i i 40 5 9 Changing the specimen or interrupting Sectioning cccceseesecseesssesecscsessrsseceesessnseeseseseesanees 40 Optional accessories ccccccccscssesessssscssssssssescsssssssscscsseesesscecsese
30. been reached The specimen was already within the residual path when the instru ment was switched on The motorized sectioning pro cess Is still in progress STEP mode is still active LED in SECT button lights up Foot switch was held down too long when starting the section ing process The RUN STOP and ENABLE buttons of the control panel were not released simulta neously after starting The end position detection is defective 1 2 Switch off the instrument and 2 1 Unlock the EMERGENCY STOP button and select the operating mode release the foot switch Release the handwheel lock wait 30 seconds Push the button of the automatic fuse 28 on the rear of the instrument Switch the instrument back on Connect the foot switch or dum my plug and ensure that it is connected correctly Select the trim mode by pressing TRIM SECT Then return the specimen using the coarse feed button Then retract the specimen using the coarse feed button Follow the procedure in Item 1 End motorized sectioning by pressing the foot switch or the RUN STOP or ENABLE button Press the TRIM SECT button to switch to trimming mode Return the specimen using the coarse feed button Only press the foot switch briefly to start the sectioning process Release both buttons simulta neously Contact Leica after sales service 65 7 Customized solutions
31. circuit board 72 Instruction manual V 1 4 02 2007 10 EC Declaration of Conformity CE feica EC Declaration of Conformity We herewith declare in exclusive responsibility that the Leica RM2235 Rotary Microtome was developed designed and manufactured to conform with the e Council Directive 98 37 EEC and e European council Directive 98 79 EG IVDD The following harmonized standards were applied e EN 61010 2 101 2002 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment e DIN EN ISO 12100 1 2003 Safety of machinery Basic concepts general principles for design Part 1 Basic terminology methodology e DIN EN ISO 12100 2 2003 Safety of machinery Basic concepts general principles for design Part 2 Technical principles and specifications e EN 418 1992 Emergency stop equipment Principles for design In addition the following in house standards were applied e DIN EN ISO 9001 2000 ee Leica Biosystems Nussloch GmbH 0 Postfach 1120 ae D 69222 Nussloch baii ae Da Fi cae Sw a awe January 12 2004 g p Managing Director Leica RM2255 13 People s Republik of China Administrative Measures on the Control of Pollution Caused by Electronic Products 55 3 Hi Rt Hazardous substances RITE FF a X Name of the part fa K Fa KiE IRP RAR Pb Gg Cd Cr
32. ck nut f is facing to the back Tighten the lock nut Slide the cross member d onto the column Ensure that the lock screw g is facing to the right of the baseplate The horizontal arm must be centered over the microscope Slide the horizontal arm 3 flat side facing the lock screw g into the cross member d and tighten For more information on connecting and using the microscope magnifying lens or cold light source please see the appropriate manual 59 6 Optional accessories 6 9 Magnifying lens The magnifying lens provides a 2x magnification and can be used with all Leica 2200 series rotary microtomes e Open the screw 3 on the horizontal arm of the microscope carrier in a counterclock wise direction e Insert the silver connection piece 1 as far as it will go Tighten the screw 3 e The adapter 2 allows a fiber optic light guide to be installed e Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled com pletely to the side if necessary Always protect the magnifier glass from exposure to direct sunlight Exposure to sunlight may cause a burning glass effect Danger of fire e Use the provided protective cap 4 to cover the magnifying lens 60 Instruction manual V 1 4 02 2007 6 Optional accessories 6 10 Cold light source Leica RM2255 The cold light source may be operated at the power
33. d hours per day gt 5 hours per day lt 5 hours per day Sectioning speed Predominantly high sectioning speed Slow to medium sectioning speed Specimen material Working with soft and hard specimens Predominantly soft specimen material Maintenance Every 12 months Every 24 months Note for inspection SECT e When the instrument reaches approx 2 million performed strokes the code SEr always appears for approx 3 seconds in the three digit l display when the TRIM SECT button is pressed switching between trimming and sectioning mode This is a reminder that the instrument must be inspected by an autho SEr rized Leica service technician regardless of how heavily the instru ment has been used 70 Instruction manual V 1 4 02 2007 8 Cleaning and maintenance Fig 70 Leica RM2255 8 2 3 Lubricating the instrument Once a month lubricate the following parts with the included drive parts oil No 405 1 2 drops are enough Instrument and specimen holder e The clamping key 95 of the quick clamping system e The T piece 55 on the microtome base plate e Guide rails 96 for the knife holder base on the microtome base plate e The T piece on the knife holder base 56 Knife holder E e Clamping lever 11 for the lateral displace ment e T piece 88 and guide 97 of the knife holder for the lateral displacement e Clamping lever 10 for the blade Knife holders N and NZ
34. e as well as various settings All operating functions are centrally located in the separate control panel All keys and displays are logically arranged in functional groups and easily identifiable 5 1 1 Instrument control panel M STOP LED Lights up when handwheel locking mechanism is activated Green LED E Lights up when trim ming mode is activat ed Green LED E Lights up when sec tioning mode is activat ed Green LED Section thickness sum indicates the total for all cuts E STOP LED Lights up when emergency stop function is activated RETRACT LED Lights up during specimen retraction Three digit display for displaying section thickness trim ming section thickness Four digit display of the section counter Green LED Section counter displays the number of all sections CLEAR button Resets the display section counter or section thickness sum back to 0 MENU MODE button Switches between section thickness sum and section counter MENU MODE CLEAR pressed simultaneously switches to the setting of the retraction value Fig 14 22 Instruction manual V 1 4 02 2007 5 Operation 5 1 2 Control panel Buttons Three digit display for for setting the section thickness section thickness eee trimming section trimming section Ke thickness thickness Green LED Lights up when trim ming mode
35. e can be caused to the instrument if the voltage selector is set to an incorrect voltage e When changing the voltage selector setting ensure that the instrument is not connected to the mains power e Connect the instrumentto a grounded power socket only using one of the power cables provided Do not interfere with the grounding function by using an extension cord without a ground wire e The instrument is ready for operation only if a dummy plug or foot switch optional is connected If you hear a beeping sound continuous after switching on the instrument check that the control panels are connected correctly e Do not operate in rooms with explosion hazard e Exposure to extreme temperature changes between storage and installation locations and high air humidity may cause condensation inside the instrument If this is the case wait at least two hours before switching on the instrument Failure to comply with this may cause damage to the instrument e The protective devices on both instrument and accessories must neither be removed nor modified A e Once removed from the crate the instrument may only be transported in an upright position Personal safety precautions e When working with microtomes personal safety precautions must always be taken It is mandatory to wear work safety shoes safety gloves a mask and safety goggles 8 Instruction manual V 1 4 02 2007 2 Safety Warnings Working at the i
36. each section While the specimen is in retraction the yellow LED on the RETRACT display 4 lights up E Switching off the specimen retraction e To call up the retraction settings press MENU MODE and CLEAR Menu P a simultaneously M Clear ode e To switch off retraction press the button on the control panel until the display indicates OFF _ j vo e To exit the retraction settings press MENU MODE When retraction is switched off the specimen Is not retracted The yellow LED 4 of the RETRACT indicator does not light up Leica RM2255 27 5 Operation Setting the sectioning speed e The sectioning speed can be set continuously in the range of 0 420 mm s using the rotary knob The speed selector has a scale graduation from 1 to 10 The graduation Is provided for reference purposes and does not indicate a particular speed The set sectioning speed is only active within the boundaries of the sectioning area A higher speed is used outside the sectioning area Sectioning window setting The sectioning window function allows to optimally adapt the size of the sectioning area to the actual size of the specimen The selected sectioning window setting is maintained when the instru ment is turned off e Turn the handwheel to position the lower edge of the sample approxi mately 3 mm above the cutting edge e Press the SET SECTIONING WINDOW button This defines
37. ed feed motion for as long as the button is pressed row button for rapid coarse feed backward movements has a lock in function To start the rapid backwards movement away from the blade press the button 9 After the button is pressed the object head is moved to the rear end position To stop the movement press any of the four coarse feed buttons The yellow LED 40 in the button flashes while the object head is in motion and remains lit continuously when the rear end position Is reached Press the button to start the slow backwards movement The travel continues as long as the button is held depressed Press the appropriate button to start a rapid or slow forward move ment The travel continues as long as the button is held depressed During the forwards movement the yellow LED 41 in the button flashes When the front end position is reached an acoustic signal is heard and the LED stops flashing and remains lit 25 5 Operation Four digit display on the instrument The four digit display is adjustable When the pm LED is lit the display shows the sum of the section thicknesses in um for all sec tions completed since the instrument was switched on Section thickness sum When the n LED Is lit the display shows the number of all previously completed sections Fig 17 e To change the display mode push MENU MODE until the LED of the desired mode is illuminated Xn e Press CLE
38. eneesseseneaees 0502 38325 Section waste tray large esssessssseseseisiririsinisrsrurnrsnsrnrnrnrererrrrrrrreenens 0502 37931 Section waste tray SMall sicacciciscescinexcnsbapesseineanssincusgadcwaasdnnnaansiavornnedss 0503 39060 Demo Box RM22xxX series ccccecescssssscsssssersseceesessrssesesersensansatsesensates 0502 38930 VAY e EE A E E E E E A E E 0502 37932 Leica brush WU a GWG Csaccccosiacerestsaredencamsantasemerencensatecenmannetcecuaed 0183 40426 63 7 Customized solutions 7 41 Instrument malfunctions Certain instrument malfunctions appear on the 3 digit LED display or are indicated through illuminated LED s on the control panel 7 1 1 Error reports lf the message E 1 follows the software version indication after switching on the instrument a loss of parameters has occurred e Press any key All parameters are then set back to the default values e Now all settings must be checked to see if the values are still correct The values may have to be reentered You can continue with your normal working routine 7 1 2 Malfunctions possible causes and troubleshooting Instrument along with possible causes and troubleshooting procedures If the malfunction cannot be remedied with any of the options listed in the table or the problem occurs repeatedly inform the Leica Service Support immediately i Following is a list of the most common problems which can arise while working with the
39. enenuraranannninannnnnnnnnrsrnrsrsrnrerens 0356 09200 Clamping ring D 25 MM sssssesessrerenerunereneruraranaraninanannnnnnrsrnrernrnrerns 0356 08320 EM specimen holder fixture bla Ck esssesssserrrrrririrrerrs 0502 29968 EM univ specimen holder sanwacisedaisececsanesieensorssinecaiwocanedstnavenvadnsndomseses 0356 10868 EM flat specimen holder sssssesssssssrrsrersrursrururunururnnnnunnnnnananinnnnnrnrerersas 0355 10405 Instruction manual V 1 4 02 2007 6 Optional accessories 6 8 Ordering information continued Leica RM2255 Special wrench f EM holder uuu ceseesecsesssssseeserssesnsseeeereeeen 0356 10869 Universal microscope Carrier complete esseere 0502 40580 Magnifying lens assembly ou cescsesesssssecsessssrssessssrssessnsassesensaees 0502 42790 Cold light sources Leica CLS 100X 100 120V 50 60HZ qu eeeseeetseeessessereeeeeees 0502 30214 Leica CLS 100X 230V 50 60F7Z aecaerectasnsecsetasarsatbenneteseeatsesnetdes 0502 30215 Leica CLS 100X 240V 50 60HZ cceecsecseessssssessssrseseseesesnees 0502 30216 Fiber optic light guide scccscissczenevsineinannsienassaiwacdysagcestinnesnanhunongedentanetl 0502 30028 Backlighting assembly n sssssssssesesssrsrsrnnerurursrurunununununenennnanannninrnrnrsrersas 0502 38719 FOGG SUV IEC N an E N ENT 050238257 Handrest for waste tray ccccccsssccsscsssccsssrssrssssessrssesessesesensesersenses 0502 38770 Cooling device assembly eeccseeesssessssrssessesessrsensesersens
40. et screw 77 against the fixed jaw 73 e Insert the foil clamp in the standard speci men clamp as shown e Turn the knurled screw 66 clockwise to clamp the foil clamp in the standard Specimen clamp 45 6 Optional accessories 6 2 4 Foil clamp type 2 The foil clamp type 2 is appropriate for large foil ribbons It is mounted in the standard specimen clamp 72 78 79 80 Fig 35 46 To open the jaws 78 and 79 lightly loosen the 3 screws 81 with an Allen key size 4 71 Insert the foil ribbon 72 from behind to position it between the movable jaw 79 and the fixed jaw 78 To clamp the foil first tighten the screw in the middle and then the other two screws 81 with the Allen key 71 Place the foil clamp 82 in the standard specimen clamp so that the beveled surface 80 on the back of the foil clamp points to the right or left Turn the knurled screw 66 clockwise to se curely clamp the foil clamp in the standard Specimen clamp Instruction manual V 1 4 02 2007 6 Optional accessories 6 2 5 Universal cassette clamp The universal cassette clamp UCC is designed to for horizontal or vertical clamping of all kinds of commercial cassettes e Push the lever 60 forwards e Mount the cassette 65 horizontally or vertically as required e To clamp the cassette release the lever 60 Fig 36 Universal cassette
41. etting Section thickness setting range Setting values Trimming section thickness setting range Setting values Object feed Vertical stroke Maximum sectioning area w o retraction Maximum sectioning area with retraction Specimen retraction in manual sectioning mode in motorized sectioning mode Electric coarse feed Sectioning speed Return speed Repositioning of knife holder base north south east west Maximum specimen size W x H x D Specimen orientation horizontal vertical Leica RM2255 0 50 100 um from 0 50 um 5 0 um in 0 5 um increments from 5 0 20 0 um in 1 0 um increments from 20 0 60 0 um in 5 0 um increments from 60 0 100 0 um in 10 0 um Increments 1 600 um from 1 0 10 0 um in 1 0 um increments from 10 0 20 0 um in 2 0 um increments from 20 0 50 0 um in 5 0 um increments from 50 0 100 0 um in 10 0 um Increments from 100 0 600 0 um in 50 0 um increments 28 mm 1 mm feed motion via step motor 70 mm 65 mm without specimen orientation 60 mm 5 100 um in 5 um increments can be turned off varies with the sectioning speed can be turned off 300 m s and 900 m s 0 0 5 420 mm s 10 approx 120 420 mm s 10 24mm 23 mm 50 x 60 x 40 mm 8 8 15 4 4 1 4 2 Standard delivery 16 Startup Installation site requirements e Stable vibration free laboratory bench with horizontal and even stage plate practically vibration
42. for simple position correction of the specimen surface when the specimen is clamped into place Fig 20 Display of the zero position For better display of the zero position the orien tation has two red indicators 32 When both indicators are visible and both set screws 30 31 are in zero position at the same time notch point white marking on the specimen Is In zero position i When the large standard specimen clamp 50x55 mm is used the speci men orientation of 8 in north south direction is no longer possible The usable angle is only about 4 in this case Leica RM2255 The directional specimen holder fixture may be exchanged for a_non directional fixture optional accessory Orienting the specimen A Specimen blocks must not be oriented during the retraction phase If a block is oriented during retrac tion the block will advance by the re traction value PLUS the selected sec tion thickness before the next section This may cause damage to both speci men and knife e Raise the object head to the upper end posi tion and activate the handwheel lock e To release the clamp turn the eccentric lever 29 forwards e Turn setscrew 30 to orient the specimen in north south direction Turn setscrew 31 to orient the specimen in east west direction Each complete turn of the screw inclines the specimen by 2 A total of 4 complete turns 8 a
43. free floor e No other instruments nearby which might cause vibrations e Room temperature permanently between 10 C and 35 C e OQbstruction free access to the handwheel A Never operate the instrument in rooms with explosion hazard The Leica RM2255 standard delivery includes 1 Leica RM2255 basic instrument 1 handwheel complete ccccccessesessccsssssssesessessessesesesenseseesensenees 0502 37734 texternal control PANEL cacisiecescstescsicawessassrsscmaeseniaseainettantenanerdaessenits 0502 37950 lsechon waste Tay ossosa a 0502 37931 dummy UGG sirrane einki Aiii 0443 30420 1 set of power cables consisting of 1 power Cable for Germany ssssesssssrrrsrrirrerririririnisrsrsrersrsrrrerenns 0411 36958 1 power cable for USA Canada Japan esscccenren 0411 36960 1 power cable for UK ST BU F 5A aseeseen 0411 36959 1 tool set consisting Of actcecatstonsesistitnsontinisetynidctetievtnadensisseuneneenewecaides 0502 37965 1 Allen key with handle size 5 asss 0194 04760 1 Allen key with handle SIZ 4 nasser 0194 04782 1 Allen key SIZES cc secontersedsvnssssnantepasndiovatacaeisdandnataataaimanisteiatenbet 0222 04138 1 screwdriver 3x50 186 long cccesesscsecssrseceesessrsesesersesenees 0170 11568 1 bottle 50 ml of oil for drives type 405 uo eee 0336 06086 2 TWNGTO VUSOS S Z AT nrar 6943 03201 1 Leica brush W Magnet uuu cccccecessececesersncessessrseeseserseeeenees 0183 40426 1 dust protective COVED
44. h at the rear This is followed by a beep The instrument initializes The software version is displayed in the 4 digit display for approx two seconds Then the display reads 0000 After the microtome Is switched on the display fields and LEDs of all acti vated functions are lit up on the instrument s control panel and display field The three digit LED display indicates the last value set for the section thickness or trimming thickness depending on which setting was last activated This is indicated simultaneously on the control panel and microtome The LED of the active mode here the section thickness is illuminated in green When the red LED in the E STOP field 2 on the control panel of the instrument Is illuminated either e the emergency stop function has been activated the EMERGENCY STOP button is pressed or the foot switch depressed e the dummy plug or the optional foot switch is not correctly connected to the jack or not connected at all If the yellow LED in the M STOP field 4 of the control panel is lit the me chanical handwheel lock or the handwheel brake Item 3 in Fig 3 is activated The instrument cannot be started as long as the LED is lit 21 5 Operation 5 1 Operating elements and their functions The operating functions of the microtome are divided between a control panel and a display unit on the microtome A control panel on the instrument displays the current operating mod
45. ickness setting in pm Selecting the sectioning and trimming mode Zp m D To switch between sectioning mode and trimming mode press the TRIM SECT button Whenever the button is pressed the display toggles between SECT and TRIM In the SECT display the sectioning thickness tn the range from 0 50 to 100 0 um is shown and in the TRIM display the trimming section thick ness between 1 0 and 600 um Is displayed Setting the section thickness trimming section thickness z l Coarse feed functions 24 Adjust these settings using the keys on the control panel Section thickness setting range 0 50 100 pm Setting values from 0 5 5 0um in 0 5 um increments from 5 0 20 0 um in 1 0 um increments from 20 0 60 0 um in 5 0 um increments from 60 0 100 0 pm in 10 0 um increments Trimming section thickness setting range 1 600 um Setting values from 1 0 10 0 um in 1 0 um increments from 10 0 20 0 pm in 2 0 um increments from 20 0 50 0 um in 5 0 um increments from 50 0 100 0 um in 10 0 um increments from 100 0 600 0 um in 50 0 um increments The electric coarse feed at two speeds is used for a rapid movement of the Specimen towards and away from the knife With the double arrow buttons the coarse feed operates at 900 m s with the single arrow buttons it runs at 300 m s In sectioning mode the coarse feed can be operated in two different ways defined step by step specimen feed STEP funct
46. ife edge with the knife guard prior to any manipulation of breaks e Raise the specimen to the upper end position and activate the mechanical handwheel lock e Cover the sectioning edge with the knife guard e Remove the specimen from the specimen clamp and mount a new sample to continue gt e Before cutting into a new specimen move the object head back to the rear end position 40 Instruction manual V 1 4 02 2007 6 Optional accessories 6 1 Assembly for fixture for specimen clamps Depending upon the purchase order the basic instrument is delivered with the directional or rigid fixture for specimen clamps which must be assembled first All specimen clamps avail able as accessories can be used in both fixtures for specimen clamps Before assembling the fixture for specimen clamps activate the mechanical handwheel lock Fig 28 9a 9a e Finally place the dovetail holder 2 and fasten by screwing In the 4 screws 1 using an Allen key size 3 Leica RM2255 6 1 1 Rigid fixture for specimen clamps Remove the rubber ring only after attaching the object head Screw the rigid fixture for specimen clamps 4 onto the object head 3 Remove the screw 1 attach the fixture for specimen clamps 4 onto the object head 3 from the front and tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an
47. ilver csccssssessesessssrssessesessrsesaseesensaneeees 0502 37994 Knife holder E narrow band RM22xx silver nasses 0502 37958 Knife holder E narrow band RM22xx silver with ejector 0502 40508 Knife holder E broad band RM22xx silver with ejector 0502 40509 Knife holder E narrow band Run off tray RM22XX sacs 0502 38961 Knife holder E broad band Run off tray RM22xx sees 0502 39114 Knife holder E TOC RM22xxX silver oo ceccesseesecssessssssesessrsessneeseseneaees 0502 37997 Standard specimen clamp with adapter 50x60 silver eses 0502 38005 Standard specimen clamp with adapter 40x40 silver sees 0502 37998 V insert for Standard specimen clamp silver cccesesereee ences 0502 38000 Universal cassette clamp w adapter RM2125 silver 006 0502 37999 Universal cassette clamp ice cooled eseese 0502 37793 Megaclamp assembly RM22xx SIWVE cccccccssesssesssesssecesersenees 0502 38967 Foil clamp type 1 black aisecseecuesnesesaettereseaevicsneesteanecuaaiveaeeeecet 0402 09307 Foil clamp type 2 black sssesssssssrsisrerersrurururununurunenunnrunenerininenrsrsrsrsas 0402 26922 Round specimen holder silver sssssesessrsrrrrrrrrsrrrerririrrerrisrrrersns 0502 38001 Round specimen holder 3 clamping rings silver c sees 0502 38002 Clamping ring D 6 MM sssssssesessrsrersrururunnnunurununurunananarinnnannsrnrnrnrnrnnsns 0356 08322 Clamping ring D 15 MM ssssssesessrererurunen
48. in your area Severe damage can be caused to the instrument if the voltage selector is set to an incorrect voltage Never change the setting of the voltage selector while the instrument is connected to the power supply A Instruction manual V 1 4 02 2007 4 Startup Checking the voltage continued The voltage selector is located above the main power switch on the left rear side of the instru ment Fig 12 The voltage setting is displayed in the viewing window 22 e Insert a small screwdriver into the cutout 25 and carefully pry out the insert e Remove the voltage selector housing 21 together with the fuses 23 Remove the voltage selector block 24 white and insert it again such that the correct local voltage Is displayed in the viewing window 22 e Reinsert the voltage selector housing with the block and fuses and push it in until it engages audible click Connecting the power supply e Before connecting the power cable make sure that the power switch 27 on the rear of the instrument is switched to 0 OFF e Various country specific power cables are provided with the instru ment Make sure that the power cable used has the correct plug for the power socket e Insert the connector of the power cable into the connection socket 26 and plug the power plug into the power socket A Exposure to extreme temperature changes and high air humidity may cause
49. ing illumina tion with the super mega cassette clamp Instruction manual V 1 4 02 2007 6 Optional accessories 6 6 Tray 6 7 Freezer pack Fig 50 Leica RM2255 Fig 51 The tray is mounted on the hood of the micro tome so that the small feet on the underside fit into the cutouts on the hood It is for storage of the utensils used during sectioning as well as the sectioned specimens The freezer pack consists of the freezer plate 98 and the insulation jacket 99 It is for cooling or keeping cold paraffin specimens Up to 48 standard cassettes can be cooled at the same time The specimens require approx 20 min for being cooled from room temperature approx 20 C to sectioning temperature The cooling performance depends on the ambient temperature and the volume of the Specimens being cooled The insulation jacket can be placed on the hood of the microtome in the same manner as the tray or it can be placed next to the instrument When using the freezer pack on the instrument the tray Fig 47 must be removed e Remove the freezer plate from the insulation jacket and place It in a deep freezer ideally overnight but for at least six hours at approx 23 C e Reassemble the freezer plate and insulation jacket and place them on the bench or the instrument 57 6 Optional accessories 6 8 Universal microscope carrier 58
50. ion or continuous movement of the specimen The instrument is delivered with the STEP function deactivated standard configuration Instruction manual V 1 4 02 2007 5 Operation Sectioning mode Button functions in STEP mode Multiple Single step step backward backward Multiple Single step step forward forward Trimming mode Backwards coarse feed 40 Forward coarse feed 41 Leica RM2255 In sectioning mode the user can select between STEP function step by step specimen feed and continuous specimen feed When continuous feed is selected the coarse feed buttons have the same func tions as In trimming mode The STEP function is useful for careful step by step approximation of the specimen towards the blade How to activate the STEP function In the trimming mode the coarse feed buttons operate a continu ous movement as long as the button is held down The double ar TRIM Switch the instrument on while holding the button on the control panel Likewise to deactivate switch the instrument on while pressing the button l When pressing a slow speed coarse feed button in STEP mode the specimen moves towards or away from the specimen by the value indicated on the dis play single step By short activation of a double arrow coarse feed button a single step is also effected in the appropriate direction Longer activation of the double arrow coarse feed button effects a repeat
51. ioning Reduce the sectioning speed Remove paraffin from this area on a regular basis Select a slower sectioning speed Methodically reduce the clear ance angle setting until you have found the optimum angle Check all screw and clamp connections on the specimen holder system and the knife holder If necessary tighten the levers and screws Adjust the sectioning speed and or section thickness when sectioning Select a thinner section Instruction manual V 1 4 02 2007 8 Cleaning and maintenance 8 1 Cleaning the instrument Always remove the knife blade before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife When using cleaners comply with the safety instructions of the manufacturer and the labor safety regulations of your laboratory When cleaning the outer surfaces do not use xylene scouring powders or solvents containing acetone or xylene Xylene or acetone will damage the finished surfaces Ensure that liquids do not enter the interior of the instrument during cleaning Before each cleaning carry out the following preparatory steps e Raise the specimen clamp to the upper end position and activate the handwheel lock e Switch the unit off and unplug it e Remove the blade from the knife holder and insert it in the recept
52. is activated Green LED Lights up when sec tioning mode is acti vated CUT MODE button Rotary knob for setting the sectioning Mode selection speed Green LEDs For displaying the ac tive operating mode Button Yellow LED Set sectioning window Green LED Flashes until second sectioning window edge is set TRIM SECT button For switching between sectioning mode and trimming mode Yellow LED Lights up when motor is switched on Flashes during coarse feed backwards lights up when rear end position is reached Yellow LED Flashes during coarse feed forwards lights up when front end position is reached Green LED Lights up when the motor is switched off or stops at the next stop position Coarse feed buttons Trimming mode Coarse feed Coarse feed Coarse feed Coarse feed Start and stop buttons backward forward forward backward for motorized section fast fast slow slow ing Sectioning mode Multiple Multiple Single Single step step step step backward forward forward backward Fig 15 Leica RM2255 23 5 Operation 5 1 3 Display and control elements Three digit display This display ts located both on the instrument TAIM and on the control panel lf the SECT LED is lit up the display shows the Rae SECT section thickness setting in um RE SECT If the TRIM LED is lit up the display shows the Fig 16 trimming section th
53. mly and backwards until the knife blade is parallel to the lay on edge 39 see detailed illustration of the rear clamping chucks To clamp the knife 47 evenly screw the two knife clamping screws 49 inward rotate clockwise Lateral repositioning of the knife Leica RM2255 Push knife guard 8 to the center Loosen the clamping screws 49 by turning them counterclockwise Push the knife 47 to the left or right as required To clamp the knife 47 always tighten the clamping screw 49 first which is located on the side to which the knife has been repositioned by turning it clockwise 55 6 Optional accessories 6 4 Section waste tray 18 Fig 48 6 5 Backlighting 56 The backlighting is inserted at the front into the one piece knife holder Push the section waste tray 18 from the front to the microtome base plate 53 until it is held in place by the two magnets 39 on the front of the microtome base plate To remove the section waste tray lift it slightly and pull it off towards the back base Remove the two screws 1 using a slotted screwdriver and then remove the cover plate 2 Insert the backlighting 3 in the recess at the rear of the knife holder base Connect the plug 4 of the backlighting to the socket 5 on the microtome The backlighting illuminates once the micro tome is turned on with the mains switch NEVER use the backlight
54. mode Each change in the sense of rotation will be electronically detected and ROCK automatically converted into an advance or retraction movement of the specimen In the manual sectioning mode it is possible to use either the conventional method of completing a full handwheel rotation or to work in the rocking mode Rock Start and stop motorized sectioning e To start motorized sectioning press the RUN STOP and ENABLE buttons simultaneously after selecting the desired operating mode e While the cutting motor is running the yellow LED in the RUN STOP key lights up e To stop motorized sectioning press RUN STOP or ENABLE lf both the green and the yellow LEDs in the RUN STOP button are lit up the cutting motor is still running however it will stop in the next upper end position of the vertical stroke 45 Leica RM2255 29 5 Operation Motorized sectioning CUT MODE CUT MODE SINGLE 30 In motorized operation you can choose between three operating modes CONT continuous stroke SINGLE single stroke STEP step stroke CONT continuous stroke operating mode e Select CONT operating mode After beginning the sectioning process sectioning continues until the process Is stopped by pressing RUN STOP or ENABLE The specimen then stops automatically in the next upper end position of the vertical stroke SINGLE single stroke operating mode e Select SINGLE operating mode
55. mp mount including the pertinent clamping lever Leica Biosystems offers special prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired In this way perfect function of the device can be ensured over the course of many years Setting of the clamping system of the clamp mount on the segment arch To ensure a proper sectioning result clamp mount B must be securely clamped onto seg ment arch A The clamping is carried out using an eccentric at the lever 11 The clamping force is adjusted with the setscrew 12 on the underside of the segment arch The setting of the clamping is carried out so that the clamping lever can be ro tated to the stop with constantly increasing re sistance 52 Adjust the clamping with a 2 5 mm Allen key at the grubscrew 12 so that the lever initially bars when the lever is activated Continue turning the grubscrew 12 a little bit approx turn to the left or right then check that the lever no longer bars but also does not jam heavily The language CD features a short video film for this purpose Instruction manual V 1 4 02 2007 6 Optional accessories Knife holder E with water bath for narrow band and broad band blades 11 10 Fig 45 Knife holder E with water bath is available for both narrow band and broad band blades The knife guard on knife holder E consists of a red foldaway handle 9 To co
56. n be moved Enlarged detail Index marks for clearance angle setting e Move the knife holder until the index mark of the desired clearance angle coincides with the reference line on the knife holder base Example The enlarged detail illustration shows a clearance angle setting of 5 The recommended clearance angle setting for knife holder E ranges from a minimum of 2 5 to 5 e Hold down the knife holder in this position and retighten the screw 58 for clamping Fig 26 Leica RM2255 37 5 Operation 5 5 Clamping the specimen Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen prior to changing the specimen block and during all work breaks A Always clamp the specimen block BEFORE clamping the knife e Rotate the handwheel until the specimen clamp is in the uppermost po sition Activate the handwheel lock by allowing the handwheel handle to lock in place and then activate the brake Insert a specimen block into the specimen clamp A detailed description for inserting the specimen into various specimen clamps and specimen holders is provided in Chapter 6 Optional accessories 5 6 Clamping the knife disposable blade 38 Be very careful when handling microtome knives or blades The cutting edge is extremely sharp and can cause severe injury Carefully insert knife or dispo
57. ndwheel The handwheel has to be assembled before attempting to use the instrument The necessary parts and tools can be found in the toolkit 2a gt gt Fig 11 4 5 Electrical connections The feather key 4 is loosely placed in the handwheel shaft 1 and fixed in place with a tle rap during transport e Remove the cable tie 3 Caution Make sure not to lose the feather key e Place the handwheel 2 on the handwheel shaft 1 as shown e Tighten the screw 2a located in the center hole of the handwheel with an Allen key size 4 5 e Remove the cover foil from the self adhesive cover disk and fix the cover disk on the handwheel A extension cable without a ground wire The instrument MUST be connected to a grounded power socket Use only the power cable provided that matches the power supply outlets of the country of use Do not use an Checking the voltage The Leica RM2255 can be connected to various electrical power grids depending on voltage and frequency and for this reason is always delivered with a set of various power cables New instruments are factory set to 230 volts This is documented by a yellow label 230 VOLT on the rear of the instrument which covers the power switch and power socket 18 Before connecting the instrument to the power supply be absolutely certain to check that the voltage selector is set to the voltage in use
58. nstrument extremely sharp and can cause severe injury e Always remove the knife blade before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use e Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife e Always clamp the specimen block BEFORE clamping the knife or blade e Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen clamp prior to changing the specimen block and during all work breaks e ALWAYS turn the handwheel clockwise otherwise the brake will not work properly e Always take appropriate safety precautions when sectioning brittle specimens Specimen may splinter e Ensure that liquids do not enter the interior of the instrument during work e Do not attempt to clamp approach or orient the specimen during the retraction phase If a block is oriented during retraction prior to the next section the block will advance by the retraction value PLUS the selected section thickness This may cause damage to both speci men and knife e The handle of the handwheel must always be centered while in motorized sectioning mode Do not touch the handwheel while it is running there is a danger of injury from the hand wheel lock N e Take care when handling microtome knives and disposable blades The cutting edge is Warnings Cleaning and maintenance
59. pin 70 1 of the vee insert 70 in the hole 69 of the lower jaw 68 e Mount the sample as required e Turn the knurled screw 66 clockwise to move the movable jaw with the vee insert upward against the fixed jaw to securely clamp the sample Instruction manual V 1 4 02 2007 6 Optional accessories 6 2 3 Foil clamp type 1 The foil clamp type 1 Is appropriate both for clamping very small foil pieces and flat angular samples It is mounted in the standard Specimen clamp Fig 34 Leica RM2255 Clamping of foil pieces e Move the movable jaw 74 to the right as required by turning the set screw with an Allen key size 4 71 e Place the foil 72 between the movable jaw 74 and the fixed jaw 73 e To clamp the foil screw the movable jaw 74 against the fixed jaw 73 by using the Allen key e Insert the foil clamp 75 in the standard Specimen clamp as shown e Turn the knurled screw 66 clockwise to clamp the foil clamp in the standard Specimen clamp Clamping of flat angular samples To clamp angular samples replace the long set screw 76 with the short set screw 77 provided with the foil clamp e Unscrew the long set screw 76 to the left with an Allen key size 4 71 e Screw the short set screw 77 in the hole e Place the sample 67 between the movable jaw 74 and the fixed jaw 73 e To clamp the sample screw the movable jaw 74 by turning the s
60. ply with the safety instructions and warnings provided in this chapter Be sure to read these instructions even if you are already familiar with the operation and use 2 1 Safety notes This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument The instruction manual is an important part of the product which must be read carefully prior to startup and use and must always be kept near the instrument i lf additional requirements on accident prevention and environmental protection exist in the country of operation this Instruction manual must be supplement ed by appropriate instructions to ensure compliance with such requirements This instrument has been built and tested in accordance with the safety regulations for electrical measuring control regulating and laboratory devices In order to maintain this condition and ensure safe operation the operator must observe all the instructions and warnings contained in this instruction manual For current information about applicable standards please refer to the CE declaration of conformity on our Internet site www histo solutions com A instrument s internal components The protective devices on both instrument and accessories may neither be removed nor modified Only service personnel qualified by Leica may repair the instrument and access the 2 2 Warnings
61. r ring 5 only after having attached the cassette clamp on the object head e Screw the rigid fixture for specimen clamps 4 onto the object head 3 Remove the screw 1 place the fixture for specimen clamps 4 onto the object head 3 from the front and tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an Allen key size 4 e Insert the super mega cassette clamp from the side on the left into the dovetail guide of the rigid fixture for specimen clamps and tighten screw 1 A If the directional fixture for specimen clamps is used with the rigid knife holder base the orientation must be in position 0 and the cover for backlighting illumination must be detached Danger of collision if not observed NEVER use the super mega cassette clamp with backlighting illumination Leica RM2255 49 6 Optional accessories 6 3 Knife holder base and knife holder Fig 40 6 3 1 Knife holder base without lateral movement feature Fig 41 50 The plastic handles of all clamping levers on the instrument and knife holders can be moved to the position that is most convenient for each user Pull the grip 94 out of the lever hold it in this position and rotate it to the desired position It will then lock automatically when released Repositioning the knife holder base The one piece knife holder base
62. re object feeding to the knife is possible using the coarse feed buttons e Restart motorized sectioning The yellow LED 41 in the COARSE FEED button lights up e When the front end position is reached the sectioning process stops e Upon restart no more feed motion takes place e You can continue to work on the specimen by pressing the corre sponding coarse feed button in the rear end position HOME and con tinuing with sectioning i To do so you must press TRIM SECT to switch to trimming mode as otherwise you cannot use the coarse feed If the object head is already in the remaining feed range when the instrument is switched on an additional acoustic signal is heard after the software version is displayed e You can continue to work on the specimen by moving It back a short distance using the coarse feed buttons set trimming mode e The STEP function Is disabled in the remaining feed range Leica RM2255 31 5 Operation Foot switch optional accessory 32 SINGLE S EP The foot switch can be used to control the motorized sectioning process It also has a function that is similar to the emergency stop function Caution In addition to the foot switch all control panel functions and all buttons on the instrument continue to be active e Using the CUT MODE button select the desired operating mode CONT SINGLE or STEP on the control panel Fig 18 CONT
63. re possible in every direction The accuracy is approximately 0 5 For ease of estimation there is a white marking on the handle and a notch point that is noticeable during turning e To lock the current orientation turn the eccentric lever 29 backwards 33 5 Operation Fig 21 y Important LTA a Never turn the Wye screw more than 2 turn at a time Fig 22 34 Fine adjustment of the force balance lf another accessory of a different weight Is mounted on the object head 33 you must check whether it is necessary to readjust the force balance Checking the correct setting e Attach the new accessory and clamp the specimen e Set the object head to half the height of the vertical travel range by turning the hand wheel Fig 21 lf the object head remains in this exact position the setting Is correct lf the object head moves i e It is raised or low ered fine adjustment is necessary Failure to adjust the force balance may result in injury while working The force balance Is adjusted using the screw 34 which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome Use the Allen key pro vided size 5 with handle for the adjustment e fthe objecthead moves downwards turn the screw approx turn clockwise e Ifthe object head moves upwards turn the screw 34 approx
64. re width of the blade can be used The ejector 9a ensures safe blade changing Knife holder E Fig 43 Leica RM2255 The clamping levers on the knife holder are not interchangeable The two clamping levers 10 11 must re main in the position shown at all times as otherwise isolated malfunc tions of the knife holder can occur Clamping lever for the blade 10 at the right clamping lever for the lateral displacement 11 at the left 51 6 Optional accessories Knife holder E Note on the knife holder E Lateral displacement only for knife holder E The lateral movement feature of the knife holder base enables the use of the entire length of the blade or knife eliminating the need for readjust ing the knife holder The knife holder E consists of a segment arch A with lever 11 and the clamp mount B with lever 10 and ejector 13 The extreme left and right positions as well as the middle position are each marked with a notch point e To release the clamp rotate the lever 11 on the left side of the knife holder forwards e Move the knife holder sideways e To clamp rotate the lever 11 back The knife holder E is an important precision component the quality and precise adjustment of which have a lasting effect on the entire function of the microtome In case of malfunctions or damages to the clamp mount it is always required to replace the cla
65. sable blade into the knife holder and clamp Make sure that the blade is clamped parallel to the upper edge of the pressure plate For more information see Chapter 6 3 2 Fig 36 A detailed description for inserting the blade or the knife into the individual knife holders is provided in Chapter 6 Optional Accessories Instruction manual V 1 4 02 2007 5 Operation 5 7 Trimming the specimen 5 7 1 Trimming in manual operation l OA Use the TRIM SECT key to select the trim mode Set the desired trim section thickness Deactivate the handwheel lock and release the brake In TRIM mode use the coarse feed buttons to move the sample against the knife blade Trim the sample by turning the handwheel or Using the CUT MODE key select the ROCK operating mode and cut the Specimen with forward and backward motions of the handwheel Terminate trimming when the desired sectioning surface and depth have been reached 5 7 2 Trimming in motorized operation A The handle of the handwheel must always be centered while in Leica RM2255 Always set the sectioning speed according to the hardness of the specimen For hard specimens always select a slow speed motorized sectioning mode Always turn the handwheel evenly in clockwise direction otherwise the brake will not work properly Use the TRIM SECT key to select the trim mode Set the desired trim section thickness lf necessary
66. sassesssesesasseseeesesasseseseseraeenen 41 6 1 Assembly for fixture for specimen ClAMPS cececssecscssssssecsserssesseseesesseseecessessnseeessnseesensersnsenses 41 6 1 1 Rigid fixture for specimen clampS ccccscesssecssssssecscsrssrsseseserseesensessesensessesersonsessessnseesensesensaneas 41 Instruction manual V 1 4 02 2007 Table of contents 6 1 2 Directional fixture for SPECIMEN clamps ccccssesssccssesssesseseseseesescesseseusessesenseesesensensetsasensenees 41 6 1 3 Fine directional fixture for specimen CLAMPS cccecessessececssssscscesnssnssesesseseesesseseesansesesensanees 42 6 1 4 Rha MUG SY SEO M eissien neiaa aia ainia ea aadi Nadia Etan 43 6 2 Specimen clamps and holders ssi ceuei canon ciesndronsactscasrdoosadatnisatwibstestfipotaastonstsiueannenlestibonssaaiatirdeedia 44 6 2 1 Standard specimen clamp sssessssrsrerersrsrurrnununurunununanunanunnnannnnnnnnnunnrununununanununnnanananunanannnnnnrnnnnnnen nnns 44 B22 NCES OTE Keak A AEE I E 44 20 e TEE En Oe E E E E E E T E E E E P 45 Ze TN LINN VDE Zencani bcontdacdustensey dnndeaesaig det sind fenubstscaisiue deptaditiestcaphodpeuenteeds 46 6 2 5 Universal cassette clamp apcatonceccsrutsesaticeatreaiinasainsesacuasatestusan duonisvaedsiaanieaseumenelauiuareaternandaataenstatats 4 6 2 6 Holder for round SpeciMenS cscasnsensinstcanvenaihetsmnousnnnndsenieetaiaiutsatabousinnastoriesinaumsiadiatstinaeaseieinttsanedtesd 48 6 2 7 Super mega cassette clamp eses
67. set the sectioning window Using the rotary knob set the appropriate sectioning speed Using the CUT MODE button select the CONT operating mode continuous stoke Deactivate the handwheel lock and release the brake Start motorized sectioning and trim the sample Terminate trimming when the desired sectioning surface and depth have been reached 39 5 Operation 5 8 Sectioning displace the blade or knife in the knife holder When using the knife holder E with lateral movement it is sufficient to move the knife holder sideways i Always use a different area of the cutting edge for trimming and sectioning To do so laterally e Center the handle of the handwheel e Use the TRIM SECT key to select the sectioning mode e Adjust the appropriate sectioning thickness or verify the selected value e Depending on the operation planned use the CUT MODE key to select one of the motorized sectioning modes CONT SINGLE or STEP Always set the sectioning speed according to the hardness of the specimen For hard specimens always select a slow speed e Check the sectioning speed setting and set an appropriate speed e Start motorized sectioning e Pick up the sections and mount them on microscope slides 5 9 Changing the specimen or interrupting sectioning knife or object head as well as prior to changing the specimen block and during all work A Lock the handwheel and cover the kn
68. sily identifiable handwheel a short distance back and forth in order to create a section Leica RM2255 13 3 Instrument components and specifications 3 3 Technical data General Approvals Nominal supply voltages Nominal frequency Power draw Protective class Power fuses Pollution degree Overvoltage installation category Maximum heat emission Operating temperature range Temperature range during storage Relative humidity Humidity during storage The instrument specific marks are located next to the identification label 100 120 230 240 V AC 10 50 60 Hz 340 VA 2 x 13 2 A UL listed 2 II 340 J s 10 C to 35 C 5 C to 55 C max 80 non condensing lt 80 according to IEC 1010 UL 3101 EN 61010 Dimensions and weight Basic instrument Width including handwheel Width excluding handwheel Depth including waste tray Height total Working height knife blade Working height knife blade Weight without accessories Control panel 14 Width Depth Height Height in inclined position Weight net 413 mm 300 mm 618 mm 305 mm with tray on the hood 100 mm measured from the base plate 168 mm measured from the table approx 3 kg 121 mm 166 mm 50 mm 81mm approx 0 660 kg Instruction manual V 1 4 02 2007 3 Instrument components and specifications Microtome Sectioning thickness s
69. sssssrsrssusurururunurarunuruninanannnnnnnnununununnnununnnanananananananunnnnnnnnnnnnnnnnnnnnnne 49 6 3 Knife holder base and knife holder cc ecscseessssssessscrssesseeecsessecescesensenseesessnseeseeseseesensassesensasens 50 6 3 1 Knife holder base without lateral movement feature eseseseeeesrsrsrriririisisisisisrsrsrsrrrrrsrrrrrnrn 50 6 3 2 INNS holder E E T C ccs sata cartaatertacrnduvriusianiieiguinneienraaahestianatieastetadastedieatachuestestituatgeintaa oia hd iiaae 51 Mao KAE DOLEE N NA aae E E E E E ATA 54 64 SECUN Waslet AY noanean ea iaia ei aaa N a aiin 56 09 PAKONN aea A A E A 56 O Ua A E E E E EA 57 o TE DAC E A E A E A 57 6 8 Universal microscope carrier ssxcosdeiechcicsais daserdvecuesotstuiaucdgenoniayystvtadt phuissisiatnaduiaiuinaiadstonedsdiisiaatibnndea 58 AV CUNY IV IEN esaa T E E 60 PO Codon SO Ul CC cuca ccasteasencutannstroncasesuetactmensabsceuoiasnasudsenstcontatumuatatdeetetieispetes aabiieeiasistetagasatanecasornmues 61 Bell THIF OP Ul MMM CNS n E E E sautanasassieecgeessed aoteniaiest T 61 BZ PE MG WM OU OLY srie inti annosnteesietsaudasnesnigedadobita E E 62 7 Customized solutions si sisccccsesecsesscsaccsecteanisnedesssnsadeansctecgnnscndedainctussataacnoiaskuaesdivecpeuatenaestocseasctdades 64 PA dAstrumentmalune tions sersan E a Eara 64 TATO TOOTS a E E E 64 7 1 2 Malfunctions possible causes and troubleshooting cccccssessesserssecsesssssssesesersessesessnsensasees 64 PA FOSS aU a E T 66
70. ternet http Awww histo solutions com Leica RM2255 These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice Only in this way is It possible to continuously improve the tech nology and manufacturing techniques used in our products This document is protected under copyright laws Any copyrights of this document are re tained by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods includ ing any electronic systems and media requires express prior permission tn writing by Leica Biosystems Nussloch GmbH For the instrument serial number and year of manufacture please refer to the identification label attached to the instrument Leica Biosystems Nussloch GmbH Table of contents MECC a Cle nn ee ee ee 6 1 1 Symbols in the text and their MEANINGS ou ccccceesssscsecscsessececsesersseseesessesseseseeseesaneessesensassesneateess 6 1 2 Qualification of personnel tate spetarcsmscn cea teat aanesdseenaslconachasinnn hes anren eit nraricsaeeslatednetapnercacedireisaytonnentait 6 1 3 Intended use of instrument scassiesacsicssnessiennsscocstecacectuarienestnerecuuseamsesseipernstgivaemnatedeasntaiensiendaats 6 Fe nUn gn 812 ea en EA E E E A 6 SE a A E E 7
71. the direction of the arrow Handwheel lock manual operation only e To lock the handwheel push the lever 5 outwards and continue to turn the handwheel slowly until it locks ex actly in the 12 o clock position The LED 4 in the LOCK display lights up Caution Never operate the lever 5 during motorized sectioning Handwheel brake Using the lever 3 on the right side of the microtome base plate the handwheel can be braked In any position e To lock the handwheel pull the lever forward forcefully e To unlock the handwheel brake push the locking lever 3 back to its original position Important The LED 4 in the M STOP field indicates only that the instrument cannot be started It does not pro vide any indication that the handwheel brake is active The lever 3 must be pulled completely forward with force so that the handwheel brake is applied The handwheel is securely locked only when the lever 5 is in the 12 o clock position Instruction manual V 1 4 02 2007 Fig 5 Leica RM2255 2 Safety Centering the handle For safety reasons the handle of the hand wheel must always be centered while in motorized sectioning mode e Activate the handwheel lock e To center the handwheel lightly pull the handle 6 outwards and swivel it into the center of the handwheel 12 Fig 4 e The handle will then lock automatically when released Knife
72. the first window border e The green LED 43 in the button flashes after the first window border is defined e Pass the specimen through to the upper border of the cutting edge and y press the SET SECTIONING WINDOW again e After the second window border is defined the green LED in the button goes out This indicates that both values were accepted To set a sectioning window you must always enter a pair of values The sequence of entry upper or lower is optional Canceling a defined sectioning window SECTIONING WINDOW button once This sets the sectioning area to e To cancela defined sectioning window before the start press the SET the maximum size corresponding to the entire sectioning area 28 Instruction manual V 1 4 02 2007 5 Operation Sectioning modes ROCK The microtome can be used both in manual and motorized operation You can choose between four settings e CONT ROCK in manual mode as well as CONT SINGLE and STEP in motorized mode SINGLE i For safety reasons when the instru ment is first switched on none of the S EP operating modes Is active Fig 18 Mode selection e For selecting the mode press the CUT MODE button on the control panel until the green LED of the desired operating mode Is displayed Manual sectioning mode e Select ROCK operating mode e Turn the handwheel a short distance forwards and backwards for sectioning rocking
73. ust be pressed on the control panel are shown in the text in bold capital letters RUN STOP 1 2 Qualification of personnel The Leica RM2255 may be operated by trained laboratory personnel only All laboratory personnel designated to operate the Leica instrument must read this instruction manual carefully and must be familiar with all technical features of the instrument before attempting to operate it 1 3 Intended use of instrument The Leica RM2255 is a fully automatic motor ized rotation microtome with a separate control panel for creating thin sections of specimens of varying hardness for use in routine and research laboratories in the fields of biology medicine and industry It is designed for cutting both soft paraffin and harder specimens as long as they are suitable for being cut manually or automatically Any other use of the instrument is considered improper 1 4 Instrument type All information provided in this manual applies only to the instrument type indicated on the cover page An identification label indicating the instrument serial number is attached at the left side of the instrument feica Ace Fabr Nr 0968 10 2005 Kat Nr 050237960 100 1 20 230 240 V 50 60 Hz 340 VA Leica Microsystems Nussioch GmbH D 69226 Nussioch Made in Germany Fig 1 Instruction manual V 1 4 02 2007 2 Safety A of other Leica products Be sure to com
74. ver the cutting edge fold the knife guard handle 9 upwards as illustrated in Figure The clamping levers on the knife holder are not interchangeable The two clamping levers 10 and 11 must remain in the position shown at all times as otherwise isolated malfunctions of the knife holder can occur the left Clamping lever for the blade 10 at the right clamping lever for the lateral displacement 11 at Use Floating thin paraffin sections for example for subsequent immunostaining procedures on the surface of the water Flat paraffin sections can be removed from the surface of the water using glass slides Leica RM2255 The vessel is filled with water up to the blade After trimming remove the section waste from the tray and create the sections to be prepared The sections floating on the surface of the water can be removed using the glass slide 53 6 Optional accessories 6 3 3 Knife holder N NZ i The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives profile c and d up to 16 cm long The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times Mounting the knife support bar e Push knife guard 8 to the center e Set the knife support bar 46 onto the height adjustment screws not visible in the position shown The flat ends of the height adjustment screws must be located in the slots at e

Leica RM2255 - Research Programs & Labs

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Leica RM2255 - Research Programs &amp; Labs

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Leica RM2255 - Research Programs & Labs