- Frank`s Hospital Workshop
Contents
1. argusX1 Quick Start Manual BIOTEC FISCHER GmbH Daimlerstrasse 6 35447 Reiskirchen Tel 49 6408 6072 Fax 49 6408 64165 e mail info biotec fischer de Short operating instruction for Bio Imaging Control Software argus X1 Version 4 5 To this means of work This short operating instruction is meant for first and re users to enable them a faster start with the argusX1 software made by biostep Page 1 of 25 Start of the program Image creation After having installed the software properly see separate installation instructions press the button argus X1 on the desktop or go over the program menu of Windows for starting the software argus AA After that the program window of argusX1 appears on the monitor Window Database Menu bar Symbol bar of the Image Processing window main functions current loaded image File Biolmaging Help AN PE 73 Bio Imaging E E Demo Images Gels E 1D gel standard fi Autoradiogramm CoomassieBlue gel E CoomassieBlue gel b w CoomassieBlue mini gel Fluorescence gel amp 4 Fluorescence gel b w a Gene array P SilverStain gel zZ Test gel high resolution i amp 4 Test gel minimal resolution C Images for quality control Macro acquisition 23 Microscopy Colonies C Pictures Coom ssieBlue gel Preview window Symbol bar Image processing Status bar The following cameras can be controlled with argusX1 if their internal controller2. software has been modified by biostep Olympus C 2040 C 3040 C 4040 C 4000 C 750 C 5050 C 5060 C 7070 C 8080 For activating the camera put the choice switch of the camera on exposure mode e g A S M etc and click on the button Camera in the software Page 2 of 25 You are shown the following error message if the camera is not switched on In this case please close the software switch on the camera and restart argusX1 I a Camera Control The exposure window Is divided into 4 ranges Camera Method Operating functions for the camera control Image window Camera Method Live Image Camera Ihlethod C5050Z F 1190063 Image window ched on No camera found Please check if camera connected and Operating functions for the camera control x Standard mode Professional mode Live Image LCD est screen oom BO amp 15 8 mm or Focus Exposure Auto Aperture jae Integration m time msec 400 Overexposure control a Image Format Resolution 3200 2400 JPG x Rotate Picture Reflect Vertical B bit greyscale image a Invert image w Froceed The camera now is in an operational condition Dependent on the preset mode in the field Live mage you are now able to see a preview of your image With Preview image you are shown kind of a Live image which is represented with a low resolution 320 x 240 pixels Due to the restricted speed
3. 4200 x 360 TIFF a high resolution JPG image with a transfer time x as an essentially more badly resoluted TIF image with the same transfer time 23456 1600 1200 TIFF 2045 1536 TIFF 2200 s 1712 TIFF ASIE Both acquisitions clearly show the difference between a Test Image left and an acquisition with high resolution right with a chosen image area in the zoom window sell right upper corner Page 8 of 25 After the creation of a Test mage or an Exposure you can enlarge any range desired with the button Zoom By activating the function Zoom window an additional window is opened in the right upper corner of the mage window The size and position of the zoom window is fixed and cannot be changed In the Zoom window the currently processed image is completely shown For selecting an excerpt go with the mouse pointer into the Zoom window and draw a rectangle over the corresponding area The selected area is represented in the Processing window With the activation of the button Proceed the image in the exposure window will be transferred into the database of argusX1 Please note that the transfer of Test images is P l not supported Now close the camera control window tad Proceed Page 9 of 25 Module FireWire camera You can use CCD FireWire cameras type Felix 5000 6000 6020 and 7000 equipped with argus X1 software for the image acquisition The control and the power supply of the cameras effe
4. of transfer you get a new image only every other second This function primarily serves for the orientation of the gels blots on the transilluminator and the setting of the zoom range Page 3 of 25 Beside the possibility to control the zoom in the live image mode the following functions have a direct influence on the image acquisition e Rotate image e Reflect image e 8 Bit greyscale image e Invert image In the image on the right the movement of your gels blots into the middle of the image is shown If you work with a tripod you can move the position of your camera too This is also valid for users who work with a set top dark hood that is simply placed on a transilluminator In case you see no image at all please check the lighting conditions first switch on the transilluminator At this point it is sufficient to recognise the outlines of the gels Adjust the zoom now With this function the image area is resized to the sample which has to be exposed As all cameras controllable by argusX1 are equipped with motor zoom lenses the zoom value can be set in the software oom With the function LCD ext Monitor the LCD monitor of the camera or a monitor connected with the camera socket A V Out is activated This enables a clearly faster live image The display at the PC is deactivated than If the live image function Preview off is activated the live image is switched off and with the button Test im
5. 00 ms The maximal exposure time of the camera is 60 sec To receive a contemporary optimal image the exposure time had been reduced to 1000 ms in the automatic control cycle The exposure conditions are another reason as the mode Live image is only applied for the adjustment of the sample with white top light No exposure time above 1000 ms is expected with opened aperture There is the possibility to register manually a higher exposure time e g 1500 ms under exposure functions and to use it for Live Image display Acquisition settings Acquisition functions Name Notes Aperture zoom and focus are adjusted manually with the objective Once Device control the focus has been optimal set you can use this data in the whole zoom 7 y ode range We recommend fixing the optimal focus with the setscrew at the objective auto Integration time ms N cane Page 11 of 25 After adjusting the sample on the transilluminator and adapting the zoom range with activated white top light in the dark hood only the fluorescent light e g UV or blue light can be used for the excitation of the sample structure Please note that in connection with the activation of the fluorescent light source the white top light has to be necessarily switched off The camera is still in the mode Live image and shows you the fluorescent sample structure Acquistion setings This image can be optimized and adjusted with several functions Acquistion func
6. 19 images with an exposure time of 100 ms each has been added up Although being exposed 1900 ms only a small area is overexposed the other areas are completely quantifiable This is the result of the bit deepness of 15 bit effecting form the addition of 19 images zi Press Hom gt nt Exposure of an image with 100 ms no addition C gt Image data gt xj bit quantity 5 Date 17 04 2007 13 26 N Number of images 13 www biostep de I j ti i vib 7 ral zu m ze Addition of 19 images with 100 ms each Achieved bit deepness 15 bit Please compare the exposure of an image with an identical entire exposure time of 1900 ms without addition The achieved dynamic range comprises the already existing bit deepness of 12 bit of the camera Due to this reason the image is overexposed and not suitable anymore for the quantitative analysis No Addition 1 Img a 1900ms bit quantity BE a Camera Date 17 04 2007 18 34 00 C3 Method of exposure Number of images 1 Exposure of an image with 1900 ms no addition Achieved bit deepness 12 bit Page 16 of 25 The second function available Mu ti frame average is used for exposures with a force background or image noise A high image noise can occur e g if there is a set short exposure time with a gain of the camera more than 50 to reach a high exposure sensitivity By averaging several
7. age you can create a preview image Should the light intensity be insufficient please shift from the live image mode into the test image mode Therefore use the button Test Image Afterwards a single image is generated in which the optimal integration time is designated by the camera i e the camera integrates the light information to a representable image also under unfavourable light D lee circumstances prerequisite Exposure automatic Now you can also move the gel blot Test Image however the test image has to be repeated until the perfect position for the image is found Nevertheless the Test Image is no live image function it is used as control if the chosen settings guarantee a high quality image acquisition A resolution of 640 x 480 pixels is preset for the Test Image in order to transfer the image without a noticeable time delay that is caused at high resolution image through the transfer process from the camera to the PC Page 4 of 25 Focusing As mentioned above the function Test mage only offers an average image quality according to the limited speed however after this you can already judge whether the image will be keenly portrayed at the following exposure or not one criterion is e g the separation of the bands You can also zoom in an area with rectangle in the preview image and view the edge sharpness at a selected structure Depending on your way of working the result of this first atte
8. amera Live image Camera Kamera Fels 6020 Method www biostep de g in F Er Dic An zn dele Test FO4226 Acquisition setting Acquisition funchoans Name Notes Device control auto Integration time ms e ra Bar z Another approach to the exposure of an image proved of value in practice is the fixed adjustment of the exposure time that can be defined for a special type of sample This value will be saved and be available for further exposures Acquisition settings Acquisition functions Name Notes Device control Mode Single frame v Integration time ms Gain Proceed Selecting the method Acquisition settings Acquisition functions Name Notes Device control Mode Single frame Integration time ms Sn ain 4 Proceed Activation of live image mode Acquisition settings Acquisition functions Name Notes Device control Mode Single frame id Integration time ms Gain Deactivation automatic Acquisition settings Acquisition functions Name Notes Device control Mode Single frame 2 auto a 0 7 Integration time ms Gain sy es Proceed Exposure Exposure of the image After selecting the method already containing a set exposure time the mode Live image will be started and the sample will be adjusted on the transilluminat
9. ct from a FireWire lead connecting camera and PC gt External provision of electricity is not necessary For activating the camera please press the button FireWire camera in the software If no camera type 5000 6000 6020 7000 is connected to the PC or no driver unit had been installed you will receive the following error message In this case please exit argusX1 and check the installation Initialising error of the leads as well as the driver unit The camera AFI can not be initialised Please check the installation The exposure window is divided into 4 ranges camera method operating functions for the camera control image window VV V WV camera method operating functions for the camera control Fire Wire Camera Camera Method Pare Eein Fe Kamera Felis 6020 Biolmag ng Acquisition functions Name Notes Device control Brightness Contrast Gamma Over Underexposure Rotate image Reflect image normal Size 12804960 Invert Overexposure oom men Aperture Filter EtBr filter Preview Exposure Proceed image window Page 10 of 25 The camera is in an operational condition if the image could be opened without receiving an error message All settings of the image acquisition la r described in the following can be saved as a method In this way you can recall this method at the next gel of the same type and all optimal settings are immediately at your di
10. d to this method You can change this basic name Please note that you must confirm the new name with the function Save changes Delete method otherwise the old method name remains gH Use as default Page 5 of 25 One of your created methods can be defined as standard method This definition can be changed anytime by appointing another method as standard You should always us the most frequently used method as standard as this will be automatically loaded with the start of the exposure window The function Exposure contains the settings for the aperture ate Exposure Auto and integration time exposure time Gi Aperture Integration 4 options are available time msec Overexposure control The exposure automatic function Auto automatically controls the auta Auto cooperation of aperture and integration time basing on the results of the exposure measurement This means that a combination of both is set which EA Aperture manual is applicable with the measured light circumstances These values are Ch Shutter manual indicated as blue numerical values in the function range Aperture and Integration time b Manual The semi automatic functions Aperture manual and Shutter manual require one manual value each the other value is calculated by the system based on the exposure measurement With the function Aperture manual the integration time is Sg automatically determined in dependence on the manual set value tar
11. dard export format for the export format and the storage space You might change all proposed settings or just accept them The function Print consists of 2 sub functions The function Quick print is realised with the button Print The function Choice print is activated by the triangle right to the button Print The print out of the image is based on print templates These are adjusted to a specific format e g A6 A4 contain the image that has to be printed as well as corresponding information e g name date of creation acquisition parameter notes Each template has its own specific layout and information structure Before using the function Quick print for the first time sae ssonzoo 1124s m Aut please select the standard print template in the menu sare g Math Test al 1 1 1 zoom 189 origin of image Settings File Settings Standard print template which you sum omits balanos 125A Test gel high resolution will use most frequently When activating this function the image in the processing window will be printed immediately on the selected template With the activation of the function Choice print square asked to select a print template from the list of the available ones In the following window you can decide whether the print out should be made on the chosen printer as preview or in another file In independence of the present printer A4 A6 or any format it can come to faulty print outs This als
12. e quantified for over underexposure www biostep de The sample test gel has been taken as underexposed normal overexposed left to right The functions Zoom Aperture and Filter are normal memo fields With these fields you can set the optimal value determined for a special sample type With the saving of these values also other values are saved and can be set manually and can related to for further exposures Is the sample based on the settings of the objective and the lighting time optimal displayed this lighting time can be used for the real exposure in taking over this time with a click on the triangle on the right hand side of the indicated time This value is now registered under exposure settings exposure time and the automatic readjustment of the exposure time is finished in the same step For further acquisitions of the live image only the registered exposure value will be used Now you can finally trim the aperture for the optimisation of the exposure and adjust the registered exposure time correspondingly the requirements Afterwards please press the button exposure The live image generation will be finished and triggers the generation of the image as well as whose display in the exposure window corresponding to the selected settings Page 13 of 25 If the image corresponds to your requirements you can switch off the fluorescent light sources switched on for the exposure of the image Fire Wire C
13. e saved The rest of the image will be eliminated after using the button Crop So it will not take up unnecessary saving capacity on your PC Copy into clipboard With the function Clipboard it is possible to copy your image into other Windows applications without greater inter steps Select a rectangular image area that should be exported with the function Zoom use the button Clipboard and load your data from the clipboard into your application e g WORD Page 22 of 25 Histogram In the histogram the pixel quantity for each brightness step is represented It enables a statement about the tonal value range of the image Image Gata zZ Image parameters and notes co fence de Method of capture Exposure Messung Muki measuring Hang TETA time ms m The function image parameters and notes informs m Much a Shutter 1 8 about all parameters documented at the image White adusiment Auto Zoom 20 2 mm exposure All important acquisition conditions are saved to each image and are available for a future classification and quality evaluation You can judge the reproducibility or comparability of the images Exposure control Areas in the image that are over or underexposed can be determined and indicated with the function Exposure control The parameters for this function can be changed in the menu Settings under the category mage processing Exposure control Page 23 of 25 Load a new image For creatin
14. e to get a lighter exposure Please set values lt 0 to expose the samples a shorter time to get a darker exposure Using the function Over Underexposure only makes sense if you work with exposure automatic and do not want to use any manual settings for Aperture and Integration time rm Inn De u mp S _ _ me p s E J T amp SD p _ The samples test gels have been taken with the exposure level 30 0 30 left to right All other parameters has been constant Page 12 of 25 gt Rotating image range 0 180 basic setting 0 i Corresponding to the chosen option the image is turned in the exposure window gt Reflecting image range normal vertical horizontal 2 rema __ A i i i ON vertical Corresponding to the chosen option the image is reflected in the exposure window gt Size range depends on the resolution of the camera gt Reverse range switch on switch off Use this function if you favour dark information on light background when watching fluorescent exposures gt Overexposure range switch on switch off This function helps to mark areas in the images overexposed underexposed The parameters colour fast value can be set in the menu under settings image processing light control This function is recommended when checking areas of the Sample which have to b
15. g or importing a new image you have 2 possibilities Of course you can produce another image with the camera Please follow therefore the procedure described in the short operating instruction You can also load other image data using the button mport Select the corresponding image TIFF JPEG PCX PC Paintbrush and Bitmap graphics and insert it into the database of argusX1 This is also valid for images made via scanner In argusX1 you can subject these images to the post processing archiving and export if required Page 24 of 25 Save export and print images The function Save serves for the saving of the processing steps made at the loaded image The former version will be overwritten The save process even takes place without further queries because of the database structure of argusX1 By saving the current processing conditions the data saved in the function Undo are automatically deleted With the function Export the loaded image can be saved in its actual processing conditions in a foreign format supported by argusX1 on a data carrier argusX1 automatically generates a file name when exporting the image using the name of the image in the database If the name in the database contains signs that are not admissible for Windows file names e g argusX1 will automatically replace them with an underscore argusX1 uses the values determined in the settings Fi e Settings Image processing Stan
16. ght Flaurescence lamp sources e g white light in the dark hood room light Should the automatic function can not realise a satisfying colour rendition 4 other functions for the White Adjustment are available After the creation of a Test mage you see the image in a resolution of 640 x 480 however here you can already judge the image quality e g Exposure Focus The function Test Image is made for the quick testing of your settings All settings in the Standard Mode and Professional Mode have influence on the acquisition of the Test Image except the resolution In order to finally generate the image in the desired format use the function Exposure Previously select the resolution and the required image format in the function Image Format Light bulb Dark daylight Image Format Resolution 2560 x 1696 JPG a Rotate Picture Reflect o bit greyscale image u Invert image 640 x 460 JPG 1024 x 768 JPG 1260 x 960 JPG 1600 1200 JPG 2048 s 1536 JPG 2200 x 1712 JPG Exposure The transfer time of the image data from the camera to the exposure window 3200 x 2400 JPG via USB port do not only depend on the resolution but also on the chosen format The transfer of a JPG image in the format 3200 x 2400 takes approx 640 x 460 TIFF 15 seconds at a Standard PC In contrast a TIF image in the format 2288 x 1024 x 765 TIFF 1712 takes approx 90 seconds Therefore it is more meaningful to generate
17. images the image noise is nearly eliminated and the resulting image appears considerably silent Fire Wire Camera Live image E x a Acquisition settings Kamera Felix 6020 Biolmaging vi Name Notes Device control Mode Multi frame average v auto Integration time ms u Ban r Number of images Stopp sf Exposure j Proceed 150 00 ms gt Fire Wire Camera Image not saved Be x Cam t hethar Acquisition settings Kamera Felix 6020 Biolmaging Name Notes Device control Mode Multi frame average Integration time ja Gain Humber of imade Stopp Preview pos Proceed 150 00ms gt The upper image shows a single exposure with 150 ms and is characterized by a strong image noise The second image is based on the averaging of 20 images with 150 ms each and is characterized by a low noise background Page 17 of 25 Please press the button zoom image to blow up a special area of an image By activating the function Zoom image an extra window will be opened in the right upper corner of the image window Size and position of the window corner are fixed and cannot be changed The zoom window displays the active processed image For selecting a special area please go with the mouse pointer into the zoom window and draw a rectangle over the corresponding area The area will be displayed in the processing window Furthermore the image sho
18. ion strong fluorescent bands can already be overexposed before weak fluorescent bands can even be acquired Important information Only band structures being acquired within the set exposure time can be add up Because 0 0 0 0 0 For the use of this function please enter an exposure time as well as the number of the exposures range 1 99 to be added Fire Wire Camera Image not saved Camera Method aft Kamera Felix 6020 Test FO4228 e BE Single frame m Multi frame add Fe Multi frame average Acquisition sethings Acquisition functions Name Notes Device control Mode Muulti franme add auto Integration time ma L gain Number of images Acquisition functions Name Notes Device control Mode Multi frame add Page 15 of 25 Integration time ms DO Gain auto r Proceed Pressing the button exposure starts the activation of the operation You can see the numbers of the already added images on the bottom of the left angle With pressing the button Stop the operation will be finished The images being added up until break are used for the final exposure displayed in the exposure window Fire Wire Camera Live image sale Beganben terra famine Feb bl TT lt METER The left picture shows the exposure of an image with an exposure time of 100 ms no nn Images have been added se 0 r In the picture below mentioned
19. luence on the acquisition in the context of the exposure functions Auto Aperture manual und Shutter manual The function Over Underexposure is a manual exposure control with a value range of 2 exposure levels Set the values in the direction in which the camera should change the exposure Use the values gt 0 should the exposure become lighter use the values lt 0 should it become darker The sample test gel was taken with the exposure levels 2 0 and 2 left to right All other parameters were constant Using the function Over Underexposure only makes sense if your work with exposure automatic and do not want to use any manual settings for Aperture and ntegration time With the function Exposure control areas in the image can be determined and displayed which are over or underexposed The parameters for this function can be changed in the menu Settings under Image processing Exposure control ne f h za mu j i gt an r T jamen ee ite u Li _ d eS os A a The sample test gel is represented as underexposed normal and overexposed acquisition left to right Page 7 of 25 The function White Adjustment is an interesting function for light field applications in order to take influence on the image quality auto Auto Bright daylight The White Adjustment provides a colour rendition true to life at different li
20. mpt will be different When working with a tripod and sufficient lighting conditions in a darkroom hood the Autofocus of the camera will provide the correct settings and you can work on immediately The activation of the autofocus will be carried out in the field Focus When using a dark hood it can happen depending on the rw lighting conditions within the hood that the autofocus does not r provide any usable results so that you have to correct it manually In this case choose the option Manual and a new value in the field Focus and then confirm your settings with the button Test Image Repeat this process until the result is satisfying Due to the fact that the focus value depends on the zoom you can not use the manual focus value for all applications Should your further gels blots have almost the same size you can use the determined focus value as a reference value This reference value and all other settings in the operating functions can be saved as a method In this way you can recall this method at the next gel of the same type and all optimal settings are immediately at your disposal Method Biolmaging Open the menu window Methods with a click of the left mouse button on __ the white triangle and choose the function Save as new method ER The current chosen parameters and settings are applied as new method 8 Save as new method and a standardised basic name structure new method date time is assigne
21. o depends on the available report formats and can individually be adjusted Edition mode You might design your individual print template Should you face any difficulties please do not hesitate to contact us Please phone us 49 6408 6072 or e mail us to info biotec fischer de we will be glad to be of assistance In the sense of short operating instructions an exhausting explanation is unfortunately impossible due to the multiplicity of variations of printers formats and customer wishes Page 25 of 25
22. of the aperture and it is shown as blue information value i Megratori time msec 1600 Overexposure control Conversely with the function Shutter manua the aperture is SE automatically determined in dependence on the manual set value of the time and it is shown as blue information value z Aperture Integration time msec 3200 Overexposure control u The completely manual control of the aperture and integration SEE ne time can be realised by the function Manual In this function sr you can insert values in dependence on the value range of the a Integration 92 used camera optimise them step by step and save them in a time msec method Due to this the manual values are also reproducibly available for further exposures Overesposure control With this function you can reach the maximum of the detection sensitivity at light weak gels fully opened aperture longest integration time In order to look at the effects of the setting changes it is necessary to generate a new image with the new parameters Therefore use the function Test mage Page 6 of 25 The presented sample CoomassieBlue gel was taken with different integration times 50ms 250ms 769ms left to right all other parameters were constant Except the modification of the direct exposure settings argusXl offers you the function Over Underexposure for the improvement of the image acquisition This function has only inf
23. or and irradiated with white light top light Furthermore the settings for aperture and zoom saved in the method will be adjusted manually with the objective and checked whether the information of the filter correspond to the actual conditions Page 14 of 25 After adjusting the sample the white top light is switched off and the fluorescent light switched on The value of the exposure time saved in the method is ideal for exposures e g with fluorescent light In order that the exposure time is effective please deactivate the function auto automatic Now you can view the image based on this exposure time The fine adjustment optimisation of the image regarding overexposed areas can be quickly realized with the adjustment of the apertures values Please turn the aperture slightly rightwards increasing the sensitivity lighter image or leftwards decreasing the sensitivity darker image If the optimal settings are reached please press the button Exposure and after the image has been displayed you can switch off the fluorescent light You can not only take single images but also add up several images Multi frame add or average of several images Multi frame average The function Multi frame add is useful if dynamic ranges with max 16 bit are required as present e g 12 bit cameras By the addition of several images a gradual larger range is available for the linear acquisition of strong and weak bands Without this funct
24. rves for the inversion of the colour greyscales in the image amp EEEN S d PC UU EEUN BE HE EE EERE i B Convert into 8 bit greyscale This function serves for the transformation of a coloured image into a greyscale image The report about information loss is necessary as the colour channels of the images are deleted Therefore confirm with Yes if you really want to save the image as greyscale image Page 21 of 25 Notes The function Notes consists of the 3 following functions Annotation HH Rectangle and Line The menu for the selection of these functions can be activated with a click on the left mouse button on the triangle right to the icon Inserting the position of the annotation rectangle as well as the line is made with the left mouse button in the Image window M Ling LO Rectangle For changing the formatting you can open the continuing functions pom with a double click on the respective object 7A Edit annotation gt Remove annotation FT Object into background Arial 94pt R 0 G 0 B 255 Background colour A 255 G 255 B 128 background style Filled Angle 0 properties properties Line colour R 255 G 255 B B1 Proceed Bo The function Crop serves for the deletion of the image area outside a selected rectangle Due to that you first have to choose a rectangular area which shall furthermore be at your disposal and b
25. sposal Open the menu window Methods with a click on the left mouse button on the white triangle and choose the function Save as new method ais ave as new method The current chosen parameters and settings are applied as new method and a standardised basic name structure new method date time Is assigned to this method You can change this basic name Please note Delete method that you must confirm the new name with the function Save changes otherwise the old method name remains EH Use as default One of your created methods can be defined as standard method This definition can be changed by appointing another method as standard anytime You should always use the most frequently used method as standard as this will be automatically loaded with the start of the exposure window The first step of the exposure of an image Is the activation of the button Live image This mode is mainly used for the adjustment of the samples e g gels blots on the transilluminator and the setting of the zoom The Live image is displayed without any delay and with full camera resolution i e the scrolling of the sample on the transilluminator is displayed without delay on the PC monitor Depending on the lighting conditions the camera tries to display an optimal image in the live image mode For that purpose the camera changes step by step the exposure time within the control cycle The control comprises an exposure time range from 1 ms up to 10
26. ting the function Zoom window an additional window is opened The size of the zoom window can be changed if necessary For selecting an excerpt go with the mouse pointer into the Zoom window and draw a rectangle over the corresponding area The selected area is represented in the Processing window a Adjust to Window With this function the size of the image in the processing window is adjusted to the size of this window Rotate Ol The function Rotate consists of the Rotate by free angle and the both selectable fixed C functions Rotate by 90 and Rotate by 180 active with the triangle on the right of the dk icon Li By activating the button Rotate you can turn the image in an arbitrary angle With the left mouse button you can set the determination of a spin axis and an arbitrary angle Page 20 of 25 El The function Reflect serves for the reflection of your image by the horizontal or vertical axis Functions of Image Processing en E eae This function serves for the post processing of an we image with different filters and image corrections Test ces reduton these functions however only use them for the jg eens improvement of the optical quality not for images meins Gamma correction which should be quantified softfocusing and sharpenin soft focusing Sharpening Filter Interference suppression Environment in pitels Relief vii IE gt Invert This function se
27. tions Name Notes Device control gt Intensity range 25 up to 75 basic setting 0 values gt 0 produce a lighter image values lt 0 produce a darker image Brightness Contrast Gamma 14 Over Underexposure gt Contrast range 50 up to 50 basic setting 0 ne G values gt 0 produce images richer in contrast values lt 0 produce images Refect image nomai lower in contrast Size 128044350 Invert 5 oll Overexposure With the functions intensity and contrast the tone range of an image can 7 z Aperture 2 8 v be changed Compared to the gamma correction the pixels are modified in the rite feierte m same way with those 2 functions gt Gamma range 0 4 up to 2 5 basic setting 1 0 The gamma value shows the allocation of the greyscales in the image The linear allocation of the greyscales is changed with the gamma correction in the way that single ranges e g weak bands are more strengthened than others The adjustments can be used for documentations and publications but only with a gamma value of 1 0 an exact quantification of the samples is possible gt Over Underexposure range 50 up to 50 basic setting 0 This function has only influence on the acquisition in the context of the exposure functions and corresponds a manual lighting time Please set values gt 0 to expose the samples a longer tim
28. ut a long training A name is automatically generated for images which were not named in the Exposure window using the segments Date and Time e g Img 2004 10 05 13 03 23 By clicking on a selected image it will be shown in the Processing window and can consequently be processed exported and or printed Filter search a Bio Imag ng Mew record Demo Images Gels 1D gel standard Autoradiogramm CoomaszieBlue mini gel Fluorescence gel brw Gene array Test gel high resolution Test gel minimal resolution G ig a E E E E CoomassieBlue Flugrescence A Fluorescence gel G SilverStain All necessary functions for the post processing of the image are available in the vertical middle bar of the program surface e Undo e Zoom window e Rotate e Reflect e Invert e Notes e Crop e Histogram e Exposure control Page 19 of 25 e Copy into clipboard e Adjust image size to window e Functions for image processing Filter e Convert into 8 bit greyscale e Image parameters and notes The function Undo enables a step wise reverse of the image processing Maximum are the last 5 processing steps after open the image or after the last save Generally valid for the post processing and display modes If modifications should be cancelled simply use the function Undo or if all processing steps should be disregarded just reload the image q Zoom Window By activa
29. wn in the exposure window can be directly printed with the function Fast print Before using this function for the first time please select the photomaster generally used with the function Seftings Acquisition settings Acquisition functions Before printing or taking over the image in the argus database you can register a Fer special sample name and detailed information of the sample in the category wenn Name Notes These notes will be saved to the image in the argus X1 database and be searched by full text search within If no name Is registered argus X1 is generating automatically a name You can assign a basic name for the automatic generation with the function Settings The function GLPis only available with the additional activated module GLP With pressing the button Proceed the image in the exposure window will be esl transferred into the database Only images taken with the function Exposure can be Proceed transferred Please note that the transfer of test images is not supported Now close the camera control window Page 18 of 25 Post processing and display modes The database window contains all images taken and imported with argusX1 These images and all their proper information are saved in the argusX1 database Formulated in another way the database window Is the representation of the saved image data in the database The design is similar to the Windows Explorer in order to use the functions witho
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