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oMALDI 2 Installation Test InstructionsRevA

1. PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc OMALDI 2 Installation Test Instructions Revision A 10 PRODUCT ION MASS SENSITIVITY 10 1 Optimization of MSMS conditions Set up the system initially as follows Laser intensity as optimized below Laser pulse rate 20 Hz Sample scanning Square pattern Run duration 60 seconds Mass scan TOF MS MS 100 to 1800 amu Product of 1758 93 amu Source Gas Parameters Range Typical Value CUR Gas 10 55 25 Compound Parameters Range Typical Value FP 60 60 DP2 20 20 CAD Gas 4 to 8 5 IQ2 20 to 20 5 CE 85to 115 90 Resolution Parameters Range Typical Value IE1 0 0 to 5 1 0 R02 9 to 10 5 9 5 IQ3 8 5 to 10 9 0 IRP 1Q3 1Q3 GRID 5 to 15 8 TFO 5 0 to 5 0 As Optimized TST 2 0 to 2 0 As Optimized LINER 4000 4000 MIRROR 750 to 1050 As Optimized PLATE 300 to 420 As Optimized GRID 280 to 450 As Optimized OFFSET 10 0 to 10 0 As Optimized Detector Parameters Range Typical Value MCP 1800 to 2400 As Optimized Using one of the ten 500 fmole ul Renin solution spots optimize the laser intensity collision energy CE and the CAD gas to obtain the maximum sensitivity of the m z 608 Renin peak PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC
2. 200 second scan PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc 11 Page 16 of 16 OMALDI 2 Installation Test Instructions Revision A FINAL COLLECTION OF OMALDI 2 PERFORMANCE DATA Upon completion of tests on the checked system the customer support engineer will perform the following actions Copy all dam files saved during the installation to a floppy and label it Make two copies of the oMALDI 2 Installation Data Log and the Laser Power Curve table Provide one copy to the customer and one to GSS at SCIEX Make a backup of all dam files saved during the installation to a floppy and label it as Installation Files oMaldi 2 Serial No Specify the appropriate model No PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc
3. following scan conditions Laser intensity Laser pulse rate Run duration Mass scan Product of TOF Product 1758 9 200 MCA scans from Sample 1 of alph a 3 57053000000000030 e 004 t0 5 07078408568999990e 0 as optimized above 20 Hz 200 seconds complete sample desorption TOF Product 100 to 1800 amu 1758 93 amu 106 110 0516 100 90 80 70 60 50 wo wo a 40 Specification 382 1117 495 1686 354 1134 608 2407 425 1252 255 0589 fy 2818 787 2321 364 1815 FOP 1460_584 1920 235 0720 39 240 1120 3289 id P i iii 40 s00 1000 miz amu Max 106 0 counts 1758 5641 1644 5495 1672 546 1400 1600 1800 TOF Product Low Concentration Renin Alpha CHC The average intensity peak of the 608 peak from the 50 fmol sample spots after scanning for no less than 200 seconds must be Intensity at 608 gt 20tc gt 200 second scan Signal to Noise 30 200 second scan PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc OMALDI 2 Installation Test Instructions Revision A 10 3 2 Product Ion Mass sensitivity Low Concentration Renin DHB Page 15 of 16 Using the optimized conditions from 10 2 collect MS MS product spectra from nine 50 fmole ul Renin solution spots Use the optimized laser
4. power from section 9 5 From the resulting spectra examine the peak at 608 amu as well as the background peaks in the 605 to 611 amu region Record the intensity and the mass resolution for the 608 amu product ion peak from the nine different sample spots Do not use the highest_and the lowest intensity replicates From the remaining seven replicates average the intensity and calculate the signal to background ratio and compare with the specifications given below Print a copy of resulting spectra to keep with the performance data Print a copy of resulting spectra to keep with the performance data sheet Use the following scan conditions Laser intensity as optimized above Laser pulse rate 20 Hz Run duration 200 seconds complete sample desorption Mass scan TOF Product 100 to 1800 amu Product of 1758 93 amu TOF Product 1759 5 200 MCA scans trom P16_50fmolRen Max 109 0 counts ae 405 1319 100 a0 547 4026 508 1944 a0 ow i K 410 0437 a gt Ay i z 60 GAPE 7671799 50 1759 4293 T E a 354 039 756 4105 30 B30 apeo 17604366 20 pi Ji 975 2701 251 0885 He IF RAAI A 200 400 600 800 1000 1200 1400 1600 1800 miz amu 2000 TOF Product Low Concentration Renin DHB Specification The average intensity peak of the 608 peak from the 50 fmol sample spots after scanning for no less than 200 seconds must be Intensity at 608 gt 20 tc gt 200 second scan Signal to Noise 25 gt
5. 512 6301975 1643 5479 70 3240 235 0793 1148 3351 800 1000 1200 1400 1600 1800 miz amu TOF Product High Concentration Renin Alpha CHC matrix Specification The average intensity from the seven sample spots for the m z 608 product ion peak must be Intensity at 608 gt 200 tc 60 second scan Signal to Noise gt 30 60 second scan PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 14 of 16 OMALDI 2 Installation Test Instructions Revision A 10 3 Product Ion Mass Sensitivity Low concentration 10 3 1 Product Ion Mass Sensitivity Low concentration Renin Alpha CHC Using the optimized conditions from 10 2 collect MS MS product spectra from nine 50 fmole ul Renin Alpha CHC solution spots From the resulting spectra examine the peak at 608 amu as well as the background peaks in the 605 to 611 amu region Record the intensity and the mass resolution for the 608 amu product ion peak from the nine different sample spots Do not use the highest and the lowest intensity replicates From the remaining seven replicates average the intensity and calculate the signal to background ratio and compare with the specifications given below Print a copy of resulting spectra to keep with the performance data Print a copy of resulting spectra to keep with the performance data sheet Use the
6. NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 13 of 16 OMALDI 2 Installation Test Instructions Revision A 10 2 Product Ion Mass Sensitivity High concentration Using the optimized conditions from 10 1 collect MS MS spectra from each of the remaining nine 500 fmole pl Renin Alpha CHC spots From the resulting spectra examine the peak at 608 amu as well as the background peaks in the 605 to 611 amu region Record the intensity and the mass resolution for the 608 amu product ion peak from the nine different sample spots Do not use the highest and the lowest intensity replicates From the remaining seven replicates average the intensity and calculate the signal to background ratio and compare with the specifications given below Print a copy of resulting spectra to keep with the performance data Use the following scan conditions Laser intensity as optimized above Laser pulse rate 20 Hz Sample scanning Square pattern Run duration 600 seconds Mass scan TOF MS MS 100 to 1800 amu Product of 1758 93 amu TOF Product 1758 9 60 MCA scans from Sample 1 of TOF Product 1758 9 60 MCA scans from Sample 1 of a Max 575 0 counts a 3 57053000000000030e 004 t0 5 0707840856899999 110 0519 495 1681 1759 5583 382 1118 608 2318 p 2 E 3 o a a 2 E 255 0618 PO 2762 787 2265 467 1gdo 56 4625 784 2
7. OMALDI 2 MS TOF System Ion Source Installation and Site Acceptance Testing Data Instructions N Applied y KS Biosystems Caps SCIEX 850 Lincoln Centre Drive Foster City CA 94404 Page 2 of 16 OMALDI 2 Installation Test Instructions Revision A Revision Log Description of Revision 05 09 2002 PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 3 of 16 OMALDI 2 Installation Test Instructions Revision A TABLE OF CONTENTS 1 PREFACE 2 INTRODUCTION NOTES 3 SOURCE UNPACKING AND INSPECTION 4 SOURCE INSTRUMENT SET UP 5 MATERIALS REQUIRED 6 SAFETY 7 VACUUM SYSTEM PRESSURE CHECK 8 SAMPLE PLATE PREPARATION 9 TOF MS SENSITIVITY AND RESOLUTION TEST 10 PRODUCT ION MASS SENSITIVITY 11 FINAL COLLECTION OF OMALDI 2 PERFORMANCE DATA PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 4 of 16 OMALDI 2 Installation Test Instructions Revision A 1 Preface The Customer Support Engineer CSE and the customer should sign the first page of the Installation and Site Acceptance Testing Data Log after verifying that all installation criteria have been met and after the customer orientation is complete The system
8. R WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 5 of 16 OMALDI 2 Installation Test Instructions Revision A 4 SOURCE INSTRUMENT SET UP Refer to the QStar Pulsar I Installation Guide and QStar Pulsar I Service Manual for further instructions 5 MATERIALS REQUIRED 5 1 Matrix solutions DHB Agilent and Alpha CHC Agilent 5 2 Sample 1 pmol l Renin Substrate Solution 100 fmol ul Renin Substrate Solution Both 1pmol ul and 100 fmol ul Renin Substrate Solution have to be diluted from 10 pmol l Renin Substrate Solution Use the Dilution solvent from API Final Test KIT The renin substrate solution consists of 10 pmol uL renin substrate and 0 1 acetic acid dissolved in a 50 50 solution of acetonitrile water As mentioned in the instructions from the API Final Test KIT P N401936 the following procedure has to be followed to prepare the 10pmol Renin Substrate Renin Substrate Standard Add 3 80 ml of Dilution solvent to the Renin vial cap and mix gently or sonicate to dissolve the material Reconstitution produces a 10 pmol ul solution of renin substrate Dilution Solvent used for Renin is the dilution solvent from the API Final Test KIT 50 50 ACN H20 with 0 1 HOAc 1 pmol and 100 fmol uL Renin dilution from 10 pmol uL stock solution 1 Dilute 10 pmol uL renin to 1 pmol uL Remove 20 uL of 10 pmol uL renin place it in 500 uL plastic vial Add 180 uL above dilution solvent Vortex the vial for 30 seco
9. ater thoroughly Rinse with Methanol HPLC grade Examine the plate if you see any sample or matrix residue oil or fingerprints on the plate repeat steps to 5 Allow the plate to dry in an area where it will not be exposed to contaminants cover the plate with a 1 L beaker if necessary 7 Recommendation in high humidity environment pre cool the plate in fridge for 5 10 minutes then buff it with a clean lint free cloth or wipe tissue and let it dry in the atmosphere avre D 8 2 1 Sample Loading Matrix DHB Premix the sample and matrix prior to loading on the sample plate Combine the matrix DHB 1 1 with each of the samples to be analyzed From stock solutions combine with equal amounts of the DHB matrix each of the following 1 pmol Renin 100 fmol Renin PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 7 of 16 OMALDI 2 Installation Test Instructions Revision A 8 2 2 Matrix Alpha CHC 8 3 8 4 Premix the sample and matrix prior to loading on the sample plate Combine the Alpha CHC matrix 1 1 with each of the samples to be analyzed From stock solutions combine with equal amounts of the Alpha CHC matrix each of the following 1 pmol Renin 100 fmol Renin Deposit a series of 1 0 microliter spots for each of the sample types to be tested Use a fresh pipette tip between eac
10. configuration should be filled out based on the configuration present on the source to be installed If an option is not present on the source N A should be entered in the blank The check lines in the form should be checked off as they are completed Spectra and chromatograms should be printed as specified in this document If an option is not present on a system the check line should not be modified For all tests use the acquisition parameters specified on the installation form Fill in data requested If data does not meet the required values the check line should not be checked until the system is able to meet the required value 2 INTRODUCTION NOTES All initial setup procedures required to ensure the functional performance and calibration of the QStar Pulsar I instrument must be performed before collecting any analytical data to validate the performance of the oMALDI 2 source The QStar Pulsar I used to collect the oMALDI 2 performance test must be known to reliably meet all site installation test requirements 2 1 The following describes on site performance tests to be completed on the oMALDI 2 QSTAR PULSAR as part of performance verification testing 2 2 The guideline performance values in this document are to be used in diagnosing hardware problems in the oMALDI 2 QSTAR PULSAR I systems The performance verification guidelines do not represent specifications for Site Acceptance The Site Acceptance specifications are clearly marke
11. d as such However if the intensity and or resolution verification guideline values described below are not met it is unlikely that the Site Acceptance specifications will be attainable 2 3 All tests are to be run on an oMALDI 2 installed on a QStar Pulsar I 2 4 Record all test results on the Installation Check list A copy of the installation check list data log and a hard copy of all test runs data acquisition data method files dam parameter settings files and calibration runs are to be given to the District Service Manager and to the LC MS Product Department 2 5 Save copies of all method files used parameter settings file and calibration instrumentdata ins file and all tests to disk These will be needed not only for the customers GLP records but will aid in future troubleshooting The name of the saved files should include at a minimum the test section number the date and unit S N 2 6 Unless otherwise noted all installation tests will be run using renin sample from the standard ABD Sample test kit ABD P N 401 936 It must be refrigerated upon arrival Renin test sample will require dilution at the customer site Consult the document in the test kit for dilution instructions 3 SOURCE UNPACKING AND INSPECTION Inspect the packaging for mechanical damage Unpack the system and inspect the system for mechanical damage PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIO
12. h sample type The final plate will have Ten spots of 500 fmol Renin Alpha CHC Twenty spots of 50 fmol Renin Alpha CHC Twenty spots of 50 fmol Renin DHB Allow the samples to air dry completely in a clean environment then load the plate into the instrument 9 TOF MS SENSITIVITY AND RESOLUTION TEST 9 1 I U Laser Power Curve Calibration Perform the first 10 steps from the Laser Power Measurement Procedure See the QStar Pulsar I Service Manual p n 1001555B Using the oMALDI server set the repetition rate at 20Hz and the attenuator steps value at 1000 steps If the oMALDI Server is Rev 2 0 or higher the attenuator steps adjustment can be done indirectly by adjusting the percentage power level value Increase the attenuator step value by 1000 steps up to 24000 steps and measure the power each time Record the values in a table like the one below and add it to the oMALDI 2 installation Data Log Date oMALDI 2 S N Power Attenuator Steps Perform the steps 14 21 from the Laser Power Measurement Procedure See the QStar Pulsar I Service Manual Selecting Calibrate Laser Power in the Operate menu brings up the Calibration Laser Power dialog box Initially a default calibration file is loaded by the software Modify the default calibration using the values obtained in the step 4 and save the file as OMALDI 2_ Installation Date Isr Note The new percentage power pairs would be different fro
13. llation Test Instructions Revision A Resolution Parameters Range Typical Value IE1 0 0 to 4 0 1 0 R02 9 to 10 5 9 5 IQ3 8 5 to 10 9 0 IRP 1Q3 1Q3 GRID 5 to 15 8 TFO 5 0 to 5 0 0 0 TST 2 0 to 2 0 0 0 LINER 4000 4000 MIRROR 750 to 1050 975 PLATE 300 to 420 325 GRID 280 to 450 340 OFFSET 10 0 to 10 0 5 0 Detector Parameters Range Typical Value MCP 1800 to 2400 2200 Use one of the 50fmole ul Renin spots to optimize the intensity of the signal at m z 1759 9 5 TOF MS sensitivity and Resolution Test Renin DHB Set up the system as follows Laser intensity Laser pulse rate 20 Hz as optimized in 9 3 1 Sample scanning Square pattern If the crystal is uniform Run duration Mass scan 120 seconds TOF MS 400 to 1800 amu Acquire to disk the spectra using the oMaldi with a Square scanning pattern nine different 50fmol Renin DHB premix spots Label the individual files 50fmol_ MS DHB_ Sample1 9 Note Try to concentrate the laser on the edges of the spot where the crystal density is highest From the resulting spectra examine the peak at 1759 amu as well as the background peaks in the 1740 to 1770 amu region Record the intensity and the mass resolution for the 1759 amu parent ion peak from the nine different sample spots Do not use the highest and the lowest intensity replicates From the remaining seven replicates average the i
14. m the ones obtained when the default calibration file was used PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 8 of 16 OMALDI 2 Installation Test Instructions Revision A 9 2 Checking the Focusing of the laser beam Matrix to be used Alpha CHC Pulse Rate 20 Hertz Laser power level 40 uJ Pulsar Frequency 7 Kilohertz Laser Scan Pattern Single point Spot 1ul of Alpha CHC on the oMALDI sample plate Select a point on the Alpha CHC matrix spot and start the laser keep the laser firing at the same point The laser should create a clear oval black spot in the Alpha CHC matrix spot within less than five second Adjust the illuminator position to verify the focus of the laser on the sample plate if the laser does not burn an oval hole in the Alpha CHC matrix spot within less than five second 9 3 Initial Adjustment of Laser Intensity 9 3 1 DHB Matrix Set the laser intensity to a power value of 30uJ Attempt to desorp the 50 fmol renin DHB premix solution in TOF MS mode scan 400 to 1800 amu Use the spotted plate supplied with the oMALDI 2 Head Increase or decrease the laser intensity in steps of 1uJ until the ionization threshold the laser power level at which some ions first appear is found The Laser Power Range is 15 to 40yJ Typical value is 30yJ 9 3 2 Alpha CHC Matrix Set the laser in
15. n of standards should be diluted to the required concentration in eppendorf tubes by a dilution factor of 10 or less This will avoid large dilution errors 6 SAFETY Refer to all applicable Material Safety Data Sheets for any necessary precautions before handling chemical solutions or solvents Before performing the tests the customer support engineer must be sufficiently trained on operation and safety procedures for the instrument and the oMALDI 2 source All safety interlocks for the oMALDI 2 must be in place prior to its use This ensures that at no time will there be any risk of laser light exposure to the operator or to anyone in the immediate vicinity of the oMALDI2 source during testing 7 VACUUM SYSTEM PRESSURE CHECK 7 1 Attach the oMALDI 2 source to the instrument in accordance with applicable installation procedure Pump down the instrument The TOF section of the instrument must reach a base pressure of 4 0 e10 6 torr with the CAD gas off and 5 0 e10 6 torr with the CAD gas set to maximum flow 12 before testing of the source can begin 7 2 The Quad analyzer region vacuum must be lower than 2 0e10 5 with CAD 1 and must not exceed 8 0e10 5 torr with the CAD 12 8 SAMPLE PLATE PREPARATION Sample plate Cleaning Procedure Drop a few drops of soap solution dish wash detergent solution on the dirty sample plate Rub softly and thoroughly with lint free cloth or wipe tissue to clean Rinse with deionized water no tap w
16. nds and centrifuge for 30 seconds 2 Dilute 1 pmol uL renin to 100 fmol uL Withdraw 20 uL of 1 pmol uL renin solution with 20 uL pipetter and place it in another 500 uL plastic vial Add 180 uL dilute solvent to make 200 uL of 100 fmol uL solution Vortex it for 30 seconds and centrifuge it for 30 seconds 5 3 Materials used for Sample plate preparation and Sample deposition Variable Pipettes 0 1 to 1 0 ul 20 ul and 200 ul Disposable Pipette tips 1 to 2 ul 200ul Methanol HPLC Grade De ionized distilled water DDW H20 Soap solution 2 3 drops of kitchen detergent in 200 ml de ionized distilled water Latest Version of Analyst QS with oMALDI Support oMALDI 2 Performance Test Data Log Gases ZERO Air lt 2 0 ppm total CH4 50 psig and 90 psig Gas withdrawal Liquid Nitrogen 40 psig Gas withdrawal Liquid Argon 40 psig Leybold S25B or Varian DS602 Backing Pump PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 6 of 16 OMALDI 2 Installation Test Instructions Revision A COMPUTER SYSTEM REQUIREMENTS Dell Precision 220 GX300 or Precision 530 dual processor 512 Mb of RAM 20 Gb hard drive All test solutions must be kept under refrigerated conditions if for any reason they are left out of the refrigerator for longer than 24 hours new solutions must be used Stock solutio
17. ntensity and calculate the signal to background ratio and compare with the specifications given below Print a copy of resulting spectra to keep with the performance data sheet PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 10 of 16 OMALDI 2 Installation Test Instructions Revision A TOF MS 120 MCA scans from P16_SO0fmolRenin_24 TOFMS witt Max 1 8e4 counts a51 1759 4817 550 1758 4569 s500 750 FOO 650 600 550 g vi 3 o uo 5 D a E 4754 14755 4756 4767 14758 14759 4760 4761 4762 14763 14764 14765 4766 14767 17568 miz amu TOF MS 120 scans 120 sec total scan time renin 50 fmol DHB Specification The average Resolution of the 1759 peak obtained from the seven samples spots must be greater than 8000 The average intensity peak of the 1759 peak from the seven 50 fmol sample spots after scanning for 120 seconds must be Intensity at 1759 gt 200 tc 120 second scan Signal to Noise gt 10 120 second scan 9 6 TOF MS sensitivity and Resolution Test Renin Alpha CHC Set up the system as follows Laser intensity as optimized in step 9 3 2 Laser pulse rate 20 Hz Sample scanning Square pattern or manual Run duration 120 seconds Mass scan TOF MS 400 to 1800 amu Acquire to disk the spectra using the oMaldi with a Square scanning patte
18. rn nine different 50fmol Renin sample spots Label the individual files 50fmol_MS Alpha Sample1 9 PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 11 of 16 OMALDI 2 Installation Test Instructions Revision A From the resulting spectra examine the peak at 1759 amu as well as the background peaks in the 1740 to 1770 amu region Record the intensity and the mass resolution for the 1759 amu parent ion peak from the nine different sample spots Do not use the highest and the lowest intensity replicates From the remaining seven replicates average the intensity and calculate the signal to background ratio and compare with the specifications given below Print a copy of resulting spectra to keep with the performance data sheet TOF MS 119 MCA scans from Sample 1 of alpha Max 3272 0 counts a 3 5586506 1836248930 e 004 t0 1 2157342728 1758 9578 __ 1759 9575 w o o ow a E TOF MS 120 scans 120 sec total scan time renin 50 fmol Alpha CHC Specification The average Resolution of the 1759 peak obtained from the seven samples spots must be greater than 8000 The average intensity peak of the 1759 peak from the seven 50 fmol sample spots after scanning for 120 seconds must be Intensity at 1759 gt 200 tc 120 second scan Signal to Noise gt 40 120 second scan
19. tensity to a power value of 8uJ Attempt to desorp the 50 fmol renin Alpha CHC premix solution in TOF MS mode scan 400 to 1800 amu Use the spotted plate supplied with the oMALDI 2 Head Increase or decrease the laser intensity in steps of 1uJ until the ionization threshold the laser power level at which some ions first appear is found A very high laser power would not produce sample ions The Laser Power Range is 8 to 15uJ Typical value is 12pJ Note The Customer Support Engineer could use the supplied pre spotted plate to verify the oMALDI source However a new plate needs to be spotted for final installation specifications 9 4 TOF MS Optimization Setup the following conditions on the instrument use as a starting point for optimization the methods created for the renin solution in single TOF MS mode in initial installation of the Qstar Pulsar I with the Ion Spray Create a new Project named oMALDI 2_SerialNumber and save all the data and dam files in this directory Source Gas Parameters Range Typical Value CUR Gas 10 55 25 GS1 GS2 Gas 0 0 Compound Parameters Range Typical Value FP 60 60 DP2 20 20 CAD Gas 2 to 5 3 Q0 20 20 ST 14 to 17 15 1Q2 20 to 20 5 PROPRIETARY INFORMATION APPLIED BIOSYSTEMS INC NOT TO BE REPRODUCED OR DISCLOSED TO OTHERS WITHOUT PRIOR WRITTEN APPROVAL File oMALDI 2 Installation Test InstructionsRevA doc Page 9 of 16 OMALDI 2 Insta

oMALDI 2 Installation Test InstructionsRevA

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