- Frank`s Hospital Workshop
Contents
1. Gel Documentation System Gerix Installation and Operating Instructions BIOTEC FISCHER GmbH Daimlerstrasse 6 35447 Reiskirchen Tel 49 6408 6072 Fax 49 6408 64165 e mail info biotec fischer de To this means of work This manual serves to set up the PC independent system Gerix correctly The operating instructions of the transilluminator and dark hood are available for continuative use Seite 2 von 29 Directory General information and preparation Scope of delivery Preparation for system setup Setup of dark hood and transilluminator Setup of basic system Gerix 1000 Connection of b w thermal printer Mitsubishi P93E Details on safe handling of Gerix 1000 Important safety instructions Important details on system voltage Important details on UV protection Maintenance System specifications Insertion process Keyboard Functions Live Image Mode Exposure Mode Saturation Mode Automatic Illumination Invert Set Capture Mode Storage on USB sticks Print out of image displayed System Settings Load Settings Save Settings Standard Settings Date Format Set Time Date Setting of key tones and automatic storage Information Contact Details Seite 3 von 29 NBA AB A A CON N N N 1 _General information and preparation 1 1 Scope of delivery At the beginning please check the contents according to the delivery note enclosed Control unit of basic system Gerix 1000 Camera wit2. Important hint of use Do not deposit objects with sharp edges on the filter glass plate of the transilluminator They might damage the transilluminator and or cause inhomogeneous exposures Before depositing an object on the filter glass plate make sure that it is free of not hardened humid paste and other aggressive substances that might get on the filter glass plate Seite 8 von 29 3 System specifications Basic system operating voltage 100V up to 240V 50Hz 60Hz dimensions L x D x H 163 mm x 260 mm x 117 mm weight 3 2 kg control ports for camera and b w thermal printer P93E 5 7 TFT colour screen with 640 x 480 pixels keyboard with 8 keys USB port to save image data on USB stick exposure time control of camera from 0 04 sec up to 10 0 sec functions of image presentation e coloured display of over and underexposure e invert storage of 4 methodical presettings Settings Camera and objective greyscale camera for exposure of 10 seconds maximum resolution 752 x 582 pixels manual zoom objective 8 48 mm 4 Insertion process The system initialisation takes approx 5 sec after switching on and ends with displaying OK Gerix 1000 biostep GmbH Germany Thanks for the use of biostep Imaging Systems Init System OK gt O 04s Image 3 Display of successful system initialisation Seite 9 von 29 5 Display The 8 keys of the keyboard can be divided in 4 groups Control
3. O0 value range 19 O 19 Contrast CONT 0 value range 19 0 19 Gamma T 5 value range 1 5 It is based on the aspect that the image displayed is nearly identical to the print out In case you would like to change the print out the settings of brightness contrast or Gamma can be optimized until the quality requested is reached The settings for the size of print out quantity of print outs size of margin positive and negative prints can be adjusted to your requirements Please read through the manual of the printer Mitsubishi P93E Important Note In case a different b w thermal printer or video printer is used we cannot guarantee the functionality of print process as well as the quality of the print out Please inform about the connection specifications and settings of the respective printer in the manual Seite 20 von 29 7 _System Settings When pressing the key nvert Set the menu of system settings is shown for 3 sec It is structured into 4 ranges Load Settings Save Settings System Settings Information on the system The arrow keys help to navigate between the menu items being activated by pressing the key Invert Set Load Settings save Settings system Settings Information Exit gt 0 045 38 09 2008 17 39 04 Image 12 Menu of System Settings Seite 21 von 29 7 1 Load Settings One of four methodical presettings can be selected and used for the autom
4. of camera e Live Increase Exposure Time Decrease Exposure Time Auto Capture Image processing e Display of over and underexposure Invert Export of the image exposed e Save on USB Print System settings e Set Gerix 1000 Live Capture Sat Auto m de TE W Save to USB Invert Set Print Image 4 System Display Gerix 1000 Seite 10 von 29 Live Sat Auto Invert Set Capture Save to USB Print EHE ERBE Live Image Mode Exposure Mode Settings Menu Navigation Exposure Mode Settings Menu Navigation Sat Auto Invert Set Capture Mode Save to USB Print Displays images with the shortest exposure time 40 ms Raises the exposure time Navigates in lists and input fields Reduces the exposure time Navigates in lists and input fields Displays overexposed and under exposed areas in the image Automatic settings of the optimum exposure time Inverts the image System Settings Stores momentarily the active image Internal memory Freeze Stores the active image on USB stick Prints the active image on the b w thermal printer Mitsubishi P93E Seite 11 von 29 6 Functions 6 1 Live Image Mode When pressing this key the Live Image Mode is activated and the data stream with the shortest exposure time 40 ms is displayed The active image is immediately displayed regardless of the exposure time used at last Exposure Mode or the
5. on Exit gt 0 045 34 09 2008 17 44 58 Image 15 Menu Item System Settings Seite 24 von 29 7 4 Date Format The date can be displayed in 4 different formats DD MM YYYY DD MM YYYY MM DD YYYY YYYY MM DD e g 30 09 2008 e g 30 09 2008 e g 09 30 2008 e g 2008 09 30 The formats can be selected with the arrow keys and activated by pressing the key Invert Set Date Format DD MM YYYY DD MM YYYY MM DD YYYY YYYY MM DD Exit gt B 04s 38 09 2008 17 50 01 Image 16 Date Format Date Format Vaan Active Date Format DD MM YYYY DD MM YYYY MM DD YYYY YYYY MM DD Exit gt B 04s 2008 09 58 18 12 22 Image 17 Date Format changed Seite 25 von 29 7 5 Set Time Date Date and time can be set with this menu item Move the cursor shape of rhombus to the place that is to be set Set Time Date DD MM YY SR 50 09 08 18 94 39 Set Exit Cursor Rhombus to select the position gt Y 04s 38 09 2008 18 04 39 Image 18 Position to be changed By pressing the key nvert Setthe cursor changes to a triangle Now the value requested can be set with the arrow keys When the correct value is set it has be transferred by pressing the key nvert Set The cursor changes again to a rhombus Set Time Date DD MM YY HH MM SS 30 09 08 18 0639 Set Fxit Cursor Triangle to change the value gt B 04s 38 09 2008 18 06 39 Image 19 Change of position value Seite 26 vo
6. pressing a key Important Note The deactivation of the key tones has no influence on acoustic information of other system functions e g automatic setting of optimal exposure time System Settings gt Date Format Set Time Date Peep off Autosave off Exit gt 0 045 34 09 2008 17 46 37 Image 21 Setting of key tone and automatic storage Seite 28 von 29 7 7 Information This menu item displays the version of Gerix 1000 and USB firmware The information can be used required e g for service enquiries Firmware V1 10 USB Firmware V3 65 Back Exit gt Y 04s 50 09 2008 18 00 16 Image 22 Menu Item Information 8 Contact Details We wish a pleasant working and excellent results with the system If you have any suggestions or hints resulting from the work please do not hesitate to contact us We are always prepared to optimize the functions of the system Gerix1000 Please contact Biotec Fischer GmbH Tel 49 6408 6072 Daimlerstrasse 6 Fax 49 6408 64165 35447 Reiskirchen e mail info biotec fischer de Seite 29 von 29
7. 4 Unplug the components before repair and maintenance services 5 Make sure that all parts that might be touched have cooled down Plug off 2 1 2 Important details on UV protection It is very important to care for appropriate protection of skin and eyes when working with transilluminators Therefore please use UV protection glasses or a UV full face protection Shield Please use long sleeved cloths as well as appropriate gloves Caution Ultra Violet Radiation Protect your eyes Seite 7 von 29 2 2 Maintenance Periodic cleaning ensures constant high quality exposures How often your transilluminator has to be cleaned depends on various factors e g frequency of use and ambient conditions We recommend cleaning routinely at regular intervals Before cleaning unplug all components from the system voltage The cabinets of the single components can be cleaned with a damp cloth If the cabinet is very contaminated the cloth may also be damped with alcohol It is also recommended to clean carefully the cover as well as the filter glass plate with a damp cloth Please make sure that they are not cleaned with scouring or corrosive detergents as they are damaging the filter glass as well as acetone benzine and carbon tetrachloride Also avoid the use of isopropyl alcohol as it could cause stripes on the glass We recommend a cleaning with alcohol or glass cleaner All components of the system must not be dipped into water
8. Screw the filter according your application on the close up lens Seite 4 von 29 4 Connect the 12 pin connector of the control wire with the respective connector of the camera Please be very careful as no pin may be bent By correct connection the nut locks in place with a click 5 Place the round disc with the centre hole directly on the opening of the dark hood Fractionally move the disc to centre correctly over the round opening 6 Fix the camera with the screw included to the camera angle of the dark hood Use the disc and the rubber mat to achieve light tightness The respective holes are adapted to the diameter of filter and objective The rubber mat is moved over the objective in the way it fits densely to the metal sheet of the dark hood 7 Connect the control wire with the 12 pin connector of the control unit Don t forget to screw tight the coupling nut of the connector 8 Connect the control unit with a socket outlet grounded using a power cord The outlet for the power cord is at the back side of the control unit Please check again the connections before switching on Image 1 System Gerix 1000 completely set up with thermal printer P93E optional available Now the system is ready for operation and can be used to expose gels At the beginning it is recommended to work with a test gel included in the commodity Seite 5 von 29 1 5 Connection of b w thermal printer Mitsubishi P93E In case the consignment
9. activated capture mode frozen image in internal memory If the function Saturation is activated in Exposure Capture Mode it will automatically be switched off with pressing the button Live Mark for active Live Image Mode Exposure time of Live Image Mode 40 ms gt Y 04s 38 09 2008 16 52 26 Image 5 Active Live Image Mode Seite 12 von 29 6 2 Exposure Mode V With pressing the arrow keys the exposure time can be increased or decreased between the range of 0 04 sec and 10 0 sec If you press the key shortly the exposure time is changed by 0 04 sec If you press the key permanently the time changes in 0 40 sec steps The arrow keys are used to navigate in the menu Set x R TOR a t 7 vs amp ibs AAAS SUDAN h RR SONNE AN ea t x A a N N IS IR 9 N A A wa t SSS 7 oes RL 4 Fr MN Mark for Active Exposure Mode ET Me ahi VANS ae en is ARE a Exposure time of Exposure Mode e g 80 ms eX AUF 7 Dt 4 MS ia gt 9 el dj P r 8 085 __ 50 09 2008 17 21 25 Image 6 Active Exposure Mode Seite 13 von 29 6 3 Saturation Mode Automatic I llumination This key is used for 2 functions 1 Function Sat Saturation When pressing this button areas of the image that are over underexposed are displayed coloured Areas overexposed are marked in red Areas underexposed of inverted images are marked in blue This applies for all images imag
10. ated image exposure for the work with the system Gerix 1000 Prerequisite is the storage of a methodical presetting menu item Save Settings Each of the 4 methodical presettings contains settings for Exposure Time Function Over and Underexposure On Off Function Invert On Off The arrow keys help to navigate between the settings being activated by pressing the key Invert Set Load Settings Setting 1 setting 2 setting 3 Setting 4 Exit gt 0 045 30 09 2008 17 41 55 Image 13 Menu Item Load Settings Seite 22 von 29 7 2 Save Settings Up to 4 individual methodical presettings can be saved to facilitate the work of the users Four settings unassigned are available They can be selected with the arrow keys To save the settings for exposure time over and underexposure and inverting currently used press the key nvert Set Via the menu item Load Settings all presettings stored can be activated and used for work Save Settings gt Setting 1 Setting 2 setting 3 Setting 4 Exit gt 0 045 30 09 2008 17 43 15 Image 14 Menu Item Save Settings Seite 23 von 29 7 3 System Settings The system settings are structured in 4 ranges Date Format Set Time Date Key tones Peep On Off Autosave On Off These ranges can be selected with the arrow keys and activated by pressing the key Invert Set System Settings gt Date Format Set Time Date Peep on Autosave
11. es currently exposed Live and Exposure Mode and images stored Capture Mode If the LED next to the key Sat Auto is illuminated the function is activated 2 Function Auto Automatic Illumination Besides the manual setting the exposure time can also be optimised automatically If you press the key Sat Auto for approx 3 sec the automatic mode is started This is signalised with an A in the display and a blinking LED next to the key All pixels are analysed with the Gerix 1000 algorithm It increases the exposure time gradually until the first pixels 50 maximum are overexposed As soon as the optimal exposure time is reached the automatic mode will be finished You will notice this by an acoustic signal Now the system will automatically switch to exposure mode Image 7 Active Mode Automatic Illumination Seite 14 von 29 Important Note The automatic mode can be finished with the following keys anytime Arrow keys Exposure Mode gt a change in exposure time is stopped with the current value Capture gt the image is automatically stored in the latest state Invert Set gt the image is automatically inverted in the latest state Important Note If an image is overexposed it is not recommended to activate the automatic mode as the algorithm is optimised for an increment of exposure time not for a decrement Seite 15 von 29 6 4 Invert Set This key is used for 2 functions 1 F
12. h objective close up lens and filter Control wire to connect camera and control unit 3 5 mm phone jack wire Transilluminator e g UXT 20M 8K Dark hood e g DH 30 Test gel for fluorescence 3 power cords 1 2 Preparation for system setup Completely unpack and unwrap all single elements It is recommended to place them directly on a stable surface Add the control wire and the power cords to the corresponding components 1 3 Setup of dark hood and transilluminator Place the transilluminator UXT 20M 8K with the front showing to you on the working surface Make sure that the transilluminator is switched off switch at the bottom of the front side Now place the dark hood DH 30 on the transilluminator The sliding door of the dark hood must be directed to you Then mount the dark hood with 4 hinges on the transilluminator Please check carefully if each of the hinges has clicked into place correctly Connect the dark hood and the transilluminator each with a power cord and a socket outlet grounded The outlets of the components are at the backside Please check again the connections before switch on 1 4 Setup of basic system Gerix 1000 Please carry out the instructions mentioned below very accurately and conscientiously as it mainly concerns damageable and optical elements 1 Screw the objective on the camera Please pay attention as it concerns a fine thread 2 Screw the close up lens on the objective 3
13. includes the thermal printer P93E information on the connection with the system Gerix 1000 are given below 1 Place the printer below or beside the system Gerix 1000 2 Connect the BNC connector of the system with the BNC connector VIDEO IN of the printer using the BNC wire 3 Connect the 3 5mm jack plug REMOTE of the printer with the 3 5mm jack plug of the system using the jack plug wire included in the commodity 4 Connect the printer with the socket outlet using the power cord The outlet for the power cord is at the back side of the printer Please check again the connections before switching on Jeoes ae er a i i I FTSE y EEEREN i Image 2 b w Thermal printer P93E with front and back view Now the printer is ready for operation and can be used to print out the gels exposed The print process can be started with pressing the key Print on the keyboard Continuative information can be found in the manual P93E included in the scope of delivery of the printer Seite 6 von 29 2 _ Details on safe handling of Gerix 1000 2 1 Important safety instructions 2 1 1 Important details on system voltage 1 When you connect the components with the system voltage please see that a socket outlet grounded is available 2 Only connect components that are stated the correct voltage at the back with the system voltage 3 Make sure that the transilluminator is switched off during absence
14. n 29 6 6 Storage on USB stick Plug the stick in the system After connecting the stick with the control unit the USB stick is checked for enough memory capacity When checking the LED next to the key Save to USBis blinking USB remaining Images Information on active USB stick INEA Do not remove USB stick during check IEAA 30 09 2008 16 07 24 Image 10 Check for memory space of the USB stick connected After the check is completed and enough memory space is available the quantity of images to be stored on the stick is calculated and displayed in the upper right corner The LED next to the key is illuminated constantly as soon as the USB stick is approved Important Note We recommend to mainly use sticks with a memory capacity of 2 GB maximum Although the system is tested for sticks with memory capacity up to 8 GB the use of sticks up to 2 GB is advisable as results can be attained faster due to less expenditure of time used for the checking of the memory capacity Seite 18 von 29 To save the active image on the USB stick press the key Save to USB The stick is automatically activated and the image saved by Gerix 1000 The storage takes about 13 sec A bar illustrates the process During the storage process the LED next to the key is blinking USB remaining images 4332 Display of images to be stored on the stick yet USB active a Do not Remove Information on the storage process I 0 04
15. n 29 Finally the changed values need to be saved For doing this move the cursor to the command Sef and press the key nvert Set To exit the menu move the cursor to the command Ex fand press the key nvert Set Set Time Date DD MM YY HH MM SS 30 09 08 18 08 39 Set Exit gt B 04s 38 09 2008 18 07 33 Image 20 Transfer of changed values Important Note In case changed values are not to be saved or the setting of date and time is to be cancelled the menu can be exited anytime Seite 27 von 29 7 6 Setting of key tone and Autosave The key tone Peep as well as the automatic storage of settings can be activated or deactivated For doing this select the requested command with the arrow keys By pressing the key Invert Set the function requested is switched on or off The function Autosave supports fast process flows directly after system start If this function is activated the following settings will automatically be saved when switching off Exposure Time QOver Underexposure On Off Invert On Off The settings stored with Aufosave are directly activated when the system is again switch on After startup Gerix 1000 displays a live image to correctly place a sample on the transilluminator and to set e g zoom and focus The exposure time stored to expose the image is activated with the arrow keys ncreasing of exposure time Exposure Mode The function Peep de activates a signal emission when
16. s 30 09 2008 15 07 18 Image 11 Information during the storage process The folder Gerix is uniquely created on the stick when starting the storage process Subfolders with the classification YY_MM_DD Year_Month_Day are created for each day images are stored in The file name of the image to be exported corresponds to the time hh_mm_ss Hour_Minute_Second the transfer process has started The images are saved in TIF format Greyscale TIF 8 Bit with a resolution of 688 x 571 pixels The quantity of images that can be saved yet on the stick is recalculated and displayed after completing the storage Important Note When the message USB active Do not remove is displayed or the LED next to the key Save to USB is blinking do not plug the USB stick off the control unit Data loss or damage to the stick could be caused Seite 19 von 29 Print 6 7 Print out of image displayed When pressing this key the image currently displayed is printed on the thermal printer Mitsubishi P93E The image is printed corresponding to the information displayed i e all text information shown are also printed Important Note You need to make sure that the Gerix system is correctly connected to the printer P93E with the respective cord 3 5mm plug If it is not connected or if you use another printer the process has to be directly released at the printer itself The following printer settings are recommended for successful working Brightness BRT
17. unction nvert When pressing this key the currently displayed image is displayed inverted It can be displayed originally by pressing again the key 2 Function Set System settings The system settings are subdivided into 4 ranges Loading of methodic presettings Load Settings Storing of methodic presettings Save Settings Basic settings System Settings Information on the system Information When pressing the key nvert Setfor approx 3 sec the menu is shown Detailed information on the system settings can be found on pages 20 28 Mark for Active Inversion Mode gt 0 045 O 50 09 2008 16 53 45 Image 8 Active Inversion Mode Seite 16 von 29 6 5 Capture Mode With pressing this key the currently exposed image is frozen saved and the capture mode is activated If the image is saved in the internal memory no images are transferred from the camera and displayed any more Hence the sample arranged under the camera can be removed and the excitation wavelength e g UV radiation of the transilluminator be switched off An image stored in the capture mode can be checked for over underexposure with the key Sat Auto The image can also be inverted with the key nvert Set The mode can be exited with pressing again the key Capture or the key Live Mark for Active Capture Mode Stored exposure time in the Capture Mode e g 80 ms ll 8 08s 30 09 2008 15 07 18 Image 9 Active Capture Mode Seite 17 vo
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