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Cryotome FE & FSE Operator Guide

1. lt gt lt gt 353mm 1205mm 9 The Shandon Cryotome weighs approximately 125 kilograms 275 lbs get help to move it 10 If the instrument has been used with materials that are toxic or contaminated with pathogenic micro organisms follow your laboratory guidelines and the fumigation and cleaning instructions given in Chapter 6 of this Operator Guide The Product Safety Declaration in Appendix A must be completed if the instrument is to be returned to Thermo or serviced by a Thermo trained engineer 11 The instrument should be regularly cleaned as described in Chapter 6 of this Operator Guide Refer to Material Safety Data Sheets when dealing with the reagents specified in Appendix B 12 Do not use Shandon Cryotome for general refrigeration purposes such as storage of specimens Use a conventional freezer 13 Use only Thermo approved accessories or replacement parts with the Shandon Cryotome A78910100 Shandon Cryotome Operator Guide 7 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 7 e 2 1 2011 10 46 54 AM EN ee Welcome 14 Correct maintenance procedures are essential to maintain safe operation and for consistent performance It is recommended that a Maintenance Contract is taken out with your supplier 15 The instrument should be serviced annually by a Thermo tr
2. Mains inlet socket On off swith 32 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 32 e 2 1 2011 10 46 58 AM Es ee Installation and Setting Up The switch is rated for operation at 3A 250Va c 24Vd c and disconnected in circuit with a named three pin socket at the rear of the Shandon Cryotome FE and FSE The remote alarm facility is not a powered output A remote alarm connector is supplied plugged into the remote alarm socket on the rear of the instrument Use this to make connections to a remote alarm sounder If the remote alarm has been activated the middle common terminal C and right hand NC pins are connected as marked above the connector In normal operation the middle common terminal C and left hand NO pins are connected Only a technically competent person may change the remote alarm fuse or connect an external circuit to the remote alarm socket The external circuit must comply with the requirements of IEC61010 1 and IEC950 A78910100 Shandon Cryotome Operator Guide 33 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 33 e 2 1 2011 10 46 58 AM a SSE ee Controls Chapter 4 Controls The Shandon Cryotome is controlled via a touch screen display panel The images are displayed as black on a white background
3. Note The access code will be cleared s A78910100 Shandon Cryotome Operator Guide 49 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 49 e 2 1 2011 10 47 00 AM a SSE ee Controls Hold key to unlock Ke to linlock To set the hold key mode If the instrument is already in the hold key mode ie the HOLD KEY TO UNLOCK button is highlighted the selection can be confirmed by pressing the HOLD KEY TO UNLOCK button Press SELECT to return to the Setup Screen page 44 If the instrument is in the security off mode ie the SECURITY OFF button is highlighted choose the hold key mode by pressing the HOLD KEY TO UNLOCK button Press SELECT to return to the Setup Screen page 44 If the instrument is in the access code mode ie the ACCESS CODE button is highlighted choose the hold key mode by pressing the HOLD KEY TO UNLOCK button Press SELECT to confirm the selection The Enter Current Code Screen will be displayed page 52 Input the current access code and press ENTER to return to the Setup Screen page 44 Note The access code will be cleared s Access OF Feet Code Access Code To set the access code mode or to change the access code Access Code If the instrument is already in the access code mode ie the CHANGE ACCESS CODE button is highlighted press EXIT to confirm the selection and to return to the Setup Screen page 44 without changing the access code To change the access
4. If an item is selected this is inverted to white on a black background MICRONS MICRONS hpm hpm Not Selected Selected To select a button touch the relevant image displayed on the screen Use your fingertips not fingernails or sharp instruments Do not press too hard DO NOT PRESS IN MORE THAN ONE PLACE AT A TIME A FALSE KEY PRESS MAY OCCUR s e Note There is no feel when the image is pressed but there is an audible beep when a valid button is pressed If any other area of the screen is pressed there is no beep S When the display backlight is dim and the chamber light is off the instrument is in standby mode If the user touches the screen surface opens the window or operates any other control the Shandon Cryotome will sense that a user is at the instrument and will exit from standby mode The display backlight and chamber light will then come on If the instrument is not used for 15 minutes then it will revert to standby mode 34 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 34 e 2 1 2011 10 46 58 AM a SSE ee Controls Advance Retract The advance retract controls are located on the top cover to the left Controls ofthe chamber see page 1 illustration The button advances the specimens head towards the knife The button retracts the specimen head ie moves away form the knife If either button is held down for m
5. Iss4 indb 3 S es Shandon Cryotome Operator Guide e 11 34 60 67 74 88 94 95 96 2 1 2011 10 46 53 AM e ee Sym bols The following symbols and conventions are used throughout this manual and on the instrument THIS SYMBOL IS USED ON THE EQUIPMENT OR INADOCUMENT TO WARN THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE AND CORRECT OPERATION IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFERTO THIS OPERATOR GUIDE s THIS SYMBOL IS USED ON THE EQUIPMENT OR IN A DOCUMENT TO WARN THAT THERE MAY BE A BIOHAZARD ASSOCIATED WITH THE INSTRUMENT ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF THE SAMPLES USED TAKE SUITABLE PRECAUTIONS s THIS SYMBOL IS USED ON THE EQUIPMENT OR IN A DOCUMENT TO WARN THAT THERE ARE POTENTIAL PINCH POINTS IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TO THE OPERATOR GUIDE s THIS SYMBOL IS USED ON THE EQUIPMENT OR IN A DOCUMENT TO WARN THAT HARMFUL CHEMICALS ARE USED WITH THE INSTRUMENT REFER TO THE MATERIAL SAFETY DATA SHEETS FOR THE CHEMICALS USED ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF LOCAL LABORATORY PROCEDURES TAKE SUITABLE PRECAUTIONS s xe p A warning is given in the document if there is a danger of personal WARNING injury or damage to samples or equipment S Note Notes given more information about a job or instruction but do not form part of the instruction S 4 Shandon Cryotome Operator Guide A78910100 WIP Cry
6. Code The numbers will be displayed as in the code display at the top right of the screen Press ENTER to confirm the 4 digit code that was entered nme Press CANCEL to reset all the entered digits as dashes and to exit back to the Security Setup Screen The Access Code Screens are described below A78910100 Shandon Cryotome Operator Guide 51 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 51 e 2 1 2011 10 47 01 AM a SSE ee Controls Enter New Code When this screen is displayed enter a new access code Press ENTER to submit the code The screen will now change to the Re Enter New Code Screen Re enter New Code This screen checks that you have entered the code correctly Type in the same code that you entered in the Enter New Code Screen and press ENTER If this code agrees with the first code entered then the code number is valid and should be used whenever requested Enter Unlock Code This screen is displayed when the instrument has been locked and a control is moved or touched Enter the current valid access code followed by ENTER to unlock the instrument Enter Current Code This screen is displayed when the access code security mode is selected and a button for another option is pressed Enter the current valid access code and press ENTER to access different screens Note To clear the Access Code change to another security mode pages 48 51 S Defrost setup screen Defrost S
7. Iss4 indb 36 e 2 1 2011 10 46 59 AM Es NEM Controls Note When the cryobar is in boost mode the CRYOBAR button will have an asterix displayed and the text between the adjustment buttons will display the amount of time remaining for the boost cycle as shown below S CRYOBAR m ME Chamber CHAMBER QUIC This displays the actual temperature of the chamber in C Specimen SPECIMEN c t This displays the actual temperature of the specimen head in C FSE only Note When the Cryotome is in the Unload sequence the SPECIMEN button will have an asterix displayed and the text between the adjustment buttons will display the amount of time remaining for the cycle s Set SPECIMEN UNLD 04 59 ele A78910100 Shandon Cryotome Operator Guide 37 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 37 e 2 1 2011 10 46 59 AM a SSE ee Controls Options Unlock The OPTIONS and UNLOCK buttons share the same screen location but are displayed in different conditions When the instrument is unlocked the OPTIONS button is displayed Press and hold the OPTIONS for approximately 1 second to access the Options Screen page When the instrument is locked the UNLOCK button is displayed instead of the OPTIONS button The action of the UNLOCK button depends on the security mode See the Security Setup Screen page Adjuster Control Arrows Set Bpm e Up and Do
8. been damaged check the condition of the instrument Contact your dealer if there is any damage Check that the detail on the label of the packaging corresponds with your order Move the crate near to where the instrument is to be sited Cut the retaining straps and turn the three locking handles to release the packing case Remove the outer case and lower the ramp Read the label on the rear of the instrument and check that the power requirements conform with your order Retrieve the accessories pack from on top of the pallet in front of the instrument Two people are required to unload the instrument from the packing case Fit the transport handles to the back of the instrument the handles located in the foam packing at the back of the instrument Note The handles should be left attached to make sure sufficient clearance is left behind the instrument to allow for ventilation and access to the mains connector S A78910100 Shandon Cryotome Operator Guide 11 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 11 e 2 1 2011 10 46 54 AM e NENNEN Installation and Setting Up With one person on either side of the instrument ease it down the slopes off the pallet DO NOT TILT THE INSTRUMENT BACKWARDS WHEN WHEELING IT DOWN THE RAMP s Make sure that you have received all the parts listed on the packing list supplied with the instrument Contact your dealer if necessary Note Inform your dealer immediately if ther
9. between the Adjuster Control arrows is gt 0 C or 2 From the Main Screen choose OPTIONS CRYOBAR DISABLE When the cryobar is disabled by either method OFF is displayed between the Adjuster Control arrows LJ im A78910100 Shandon Cryotome Operator Guide 43 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 43 e 2 1 2011 10 47 00 AM a SSE ee Controls To enable the cryobar 1 From the Main Screen choose OPTIONS CRYOBAR ENABLE Note Ifthe cryobar was disabled by method 1 above the cryobar will cool down to a default setpoint of 30 C s Note Ifthe cryobar was disabled by method 2 above the cryobar will return to the last setpoint used For example if the cryobar was set at 40 C before it was disabled the cryobar will go to 40 C again s 2 From the Main Screen press 3 The set temperature will then decrease from C Setup Menu Setup Menu Press SETUP MENU to display the Setup Screen see below B p 1 Press EXIT to leave the Options Screen and return to the Main Screen page 36 Setup Screen Setup Menu I Setup Setup Press the required function key to display the Setup Screen for that function pages 45 53 44 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 44 e 2 1 2011 10 47 00 AM Es ee Controls Note f any change is made to the Setup screens then EXIT is re placed by SET or SELECT s ui Pr
10. cause the colour to Housekeeping fade Silver cryocassettes can be cleaned with bleach s An automatic defrost cycle takes place every day at 23 30 to clear Daily the cooling fins of any frost build up This time can be adjusted described in the Setup Screen section Extra defrosts can be activated as required from the OPTIONS screen Note 23 30 is the default time that can be changed from within the Defrost Setup screen page 52 Use the small brush and debris tray to collect and remove debris from the chamber The chamber can be wiped with an alcohol soaked cloth ALWAYS REFER TO MATERIAL SAFETY DATA SHEETS MSDS FOR REAGENTS SPECIFIED IN APPENDIX B s 66 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 66 e 2 1 2011 10 47 02 AM a SSE ee Cleaning and Maintenance Chapter6 Cleaning and Maintenance It is important that decontamination and cleaning of the instrument becomes an automatic routine especially if the source of material is unknown It is recommended that a log is kept which lists i Material Cut ii Degree of Risk iii Decontamination Performed iv Name of User v Department Cleaning isa part ofgood laboratory housekeeping practice External cleaning requirements are straightforward The refrigerated chamber should only be washed and cleaned if required after the chamber is thoroughly decontaminated by fumigation The instrument should be i
11. display lights up and the Language Screen is displayed The compressor will only start if the temperatures are set less than 0 C There may be a delay of approximately three minutes before the compressors start to operate To switch off Press the O OFF side of the 1 O switch inward to switch the instrument off 18 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 18 e 2 1 2011 10 46 56 AM a SSE ee Installation and Setting Up Fitting the Carefully locate the handwheel into the hole at the top of the right Handwheel hand side The handwheel is supplied in the accessory pack Note Ifthe specimen head is at the bottom of the stroke the handwheel handle should also be at the bottom s Make sure that the specimen head is at the top or bottom and turn the handwheel until the handle is at the top or the bottom to match Make sure that the locating pin of the handwheel fits into the key way of the drive shaft Place the spring washer and the flat washer in that order onto the handwheel bolt Insert the bolt into the recess on the handwheel and use the Allen key to tighten it turn it clockwise Fit the push in plug Rotate the handwheel and check that movement is smooth and produces a corresponding movement of the specimen head Handwheel bolt Push in plug Spring washer The lever next to the handwheel handle is used to lo
12. e 4 Finally fumes and excess moisture are extracted from the e chamber During the first part of the cycle steps 1 and 2 above the fumigation cycle can be cancelled Fumigating Defrosting and warming chamber Press CANCEL to cancel the fumigation sequence during the first two steps of the fumigation cycle The screen displayed depends on the security mode selected page 48 Follow the instructions on the 56 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 56 e 2 1 2011 10 47 01 AM Es NEM Controls screen to return to the Main Screen page 36 However once the formaldehyde is being vapourised and the fumigation cycles has been activated steps 3 and 4 above it is not possible to cancel the fumigation process The Fumigating Screen shows the time remaining for the fumigation cycle Fumigating Approximate time remainin 300 minutes The length of the fumigation cycle will depend on the Chamber Temperature at the start but will be approximately 6 hours When fumigation has ended the chamber temperature will be controlled at 5 C and the cryobar and specimen will be off The following screen will be displayed End of Fumigation lean and ary nifeholder Ceach section helves and chamber When the window is opened the following screen is displayed End of Fumigation knife and dr nifeholder Teach section helves and ch
13. earthing grounding requirements 6 electrical protection 72 electrical requirements 16 on off switch 16 wiring convention 16 electrical testing 73 flash testing 73 Portable Appliance Testing PAT 73 enter current code screen 52 enter new code screen 52 enter unlock code screen 52 environment 82 general 82 A78910100 safe operating temperature 82 transit storage temperature 82 error messages 79 error screen example 40 F fault finding error messages 79 instrument function 75 sectioning 76 78 foot rest 31 fumigate button 41 fumigating screen 55 56 fumigation 68 69 fumigation cycle 55 56 fuses 82 G grounding earthing requirements 6 H handles cryocassette 31 instrument 11 handwheel brake 19 fitting 19 harmful chemical symbol PK 4 heat extractor 31 height 61 housekeeping 66 humidity 82 installing the instrument 14 K knife holder disposable blades 22 adjusting 25 angle clamp red 23 anti roll plate 27 Shandon Cryotome Operator Guide 98 e 2 1 2011 10 47 08 AM a SSE e assembling 23 blade position clamp blue 23 fitting a disposable blade 24 fitting to the instrument 24 position clamp black 22 setting 64 L lamp fitting the tube 72 removing the tube 72 language setup screen 47 lock unlock button security off 38 M maintenance 72 main screen 36 chamber button 37 cryobar button 36 error screen example 40 microns button 36 options unlock b
14. for routine operation involves the installation and adjustment of one or more of the following i Knife holder ii Knife guard iii Disposable blade or solid knife iv Cutting angle vii Anti roll plate i e disposable blade holder comprises three sections a base middle Disposable Blade The disposable blade hold prises th b ddl Holder and top The blade holder assembly is fitted to a common support om plate attached to the base of the chamber The base section allows movement towards and away from the specimen holder Use the black lever position clamp on the left hand side to clamp the blade holder in position Position clamp BLACK 22 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 22 e 2 1 2011 10 46 56 AM Es NENNEN Installation and Setting Up The middle section can be rotated on the base section to achieve the optimum cutting angle Use the red level angle clamp on the right hand side to clamp the blade holder at the correct cutting angle Angle clamp RED The top section can be moved laterally along the middle section to use the entire length of the blade Use the blue lever blade position clamp on the left hand side to clamp the top section in position Two different top stages are available for low and high profile blades Blade position clamp BLUE Pull the red angle clamp towards you Slide the middle section onto the base se
15. guideline cutting temperatures for the Shandon Cryotome BE AWARE THAT TISSUE SAMPLES MAY POSE A BIOHAZARD TAKE SUITABLE PRECAUTIONS s Suggested cutting temperatures for frozen tissues The chamber temperature for the Shandon Cryotome FE and FSE should normally be set at 25 C but should at least be 5 10 C lower than the specimen head temperatures shown Unfixed Tissue Brain Liver Lymph Nodes Thyroid Muscle Skin Spleen Breast Breast with fat 30 or below Adipose Tissue 30 or below Fixed Tissue 12 to 17 THESE TEMPERATURES ARE GUIDELINES ONLY MAKE SURE THAT THE TISSUE IS SUFFICIENTLY A78910100 Shandon Cryotome Operator Guide 96 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 96 e 2 1 2011 10 47 08 AM Index Symbols biohazard symbol 4 harmful chemical symbol 4 pinchpoint symbol 4 warning symbol 4 WARNING warning symbol 4 do accessories 31 cryobar cover 31 cryocassettes 31 cryocassette handle 31 cryocassette identifier 31 foot rest 31 heat extractors 31 part numbers 84 remote alarm 32 shelves 31 access code screens 51 enter current code screens 52 enter new code screen 52 enter unlock code screen 52 re enter new code screen 52 advance retract controls 35 rotary control fine adjustment 35 anti roll plate 27 biohazard symbol A 4 blade holder 25 adjusting 25 angle clamp red 23 anti roll plate assembling blade position clamp blue 97 Shandon
16. heater error The fumigation heater is on permanently THE FUMIGATION HEATER IS VERY HOT DO NOTTOUCH s Shandon Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 80 e Call Thermo Service Wait for 5 minutes for the head to cool down and then reselect the desired specimen head tempera ture Remove all specimens from the cryobar and chamber and call Thermo Service Remove all specimens from the chamber and call Thermo Service Wait for 5 minutes and then reselect the desired specimen head temperature The instrument needs to be reset for the local mains voltage Call Thermo Service Call Thermo Service A78910100 2 1 2011 10 47 03 AM Specifications and Accessories Chapter8 Specification and Accessories Specification Physical Width 830mm max 33ins including handwheel Depth 745mm 29ins Height 1050 mm 411 ins working height 1205mm 275 lbs instrument height Weight 125kg 275 lbs Electrical Supply Voltages 230Va c 50Hz 1800VA 115Va c 60Hz 1800VA Voltage is set at the factory Maximum supply voltage fluctuations not to exceed 10 of nominal voltage Power demand Notes characteristics 1 I max Peak Current 2 Peak Current is for 22 secs max with stalled rotor 3 Voltage drop affects compressor start capability 4 Supply impedance affects compressor start up capability 230V 50Hz 198 253 12 1 0 115V 6OHz 103 126 38 0 25 A78910100 Shando
17. supply outlet Transit Bracket A TRANSIT BRACKET IS ATTACHED TO INTERNAL STRUCTURES TO PROTECT THE INSTRUMENT DURING TRANSPORT IT IS ESSENTIAL THAT IT IS REMOVED BEFORE AN ATTEMPT IS MADE TO OPERATE THE INSTRUMENT s Open the window to access the chamber Gradually loosen the two cap screws on the verticle part of the bracket by no more than 2 full turns Loosen the cap screw on the base if the transit bracket Lift the Shandon Cryotome head out of the bracket to its top position Gently slide the bracket towards you along the knife holder base and remove it Note Store the transit bracket for future use it is needed when the Shandon Cryotome has to be transported s A78910100 Shandon Cryotome Operator Guide 17 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 17 e 2 1 2011 10 46 55 AM a SSE ee Installation and Setting Up When the instrument is switched on the specimen head will be driven backwards to its reset position Specimen head face plat Transit bracket cap screws Transit bracket Switching on Follow these instructions to turn the instrument on and off Make ff t the transit bracket is removed before the instrument is switched and o es To switch on Press the 1 ON side of the 1 O switch inward to switch the instrument on The instrument will take a short time to initialise an hour glass symbol will be displayed on the screen Once the initialisation is over check that the
18. system and the drainage of waste fluids from the chamber Make sure that the unit is level s SITE THE INSTRUMENT IN A WELL VENTILATED AREA AWAY FROM DOWNDRAUGHTS DO NOT SITE THE INSTRUMENT NEARTO SOURCES OF DOWNDRAUGHTS SUCH AS WINDOWS OR HEATING VENTILATION DUCTS AS THE EFFICIENCY OF THE CHAMBER CAN BE AFFECTED s Use the spanner provided to turn the height adjustment knobs counter clockwise until the feet of the instrument are on the floor and the unit is stable and level Shandon Cryotome Operator Guide A78910100 Es ee Installation and Setting Up Raise the front edge of the top cover remove the transit packing from the top of the sliding windows then lower the cover back into position Push open the sliding window to gain access to the refrigerated chamber Note Make sure the window is closed before the top cover is lowered into position so that the cable is not caught up s A78910100 Shandon Cryotome Operator Guide 15 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 15 e 2 1 2011 10 46 55 AM a SSE ee Installation and Setting Up Electrical Make sure that the voltage of the mains supply corresponds with the voltage rating on the rating plate on the back of the instrument The requ irements mains supply requirements are listed in Chapter 8 Mains inlet socket On off
19. the fitting Carefully push the tube into place and rotate it by a quarter turn Electrical Protection The mains input supply feeds the Shandon Cryotome via a trip device that interrupts the electrical supply to the instrument if an internal fault occurs The trip replaces the function of a fuse When the electrical protection device trips the mains switch goes to its O position OFF and the instrument becomes inoperative If the protection circuit operates this will normally indicate a compressor problem Check that there is adequate space around the outside of the unit for cooling air and that the inlet grill on the right hand side is free of obstructions and dust Allow 1 hour for the compressor to cool before attempting to reset the trip circuit Press and release the I side of the mains ON OFF switch to switch the instrument on again If the protection circuit keeps tripping 72 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 72 e 2 1 2011 10 47 03 AM e NENNEN Cleaning and Maintenance the switch contact your Thermo Service representative ELECTRICAL PROTECTION IS INCLUDED TO PROTECTTHEOPERATORANDTHEINSTRUMENT DO NOT ATTEMPT TO OVERRIDE THE PROTECTION TRIP s WARNING Portable Appliance Testing PAT should only be carried out by a competent person It should only be carried out annually to avoid damaging the equipment s Flash testing should only be carrie
20. the instrument open the window and switch off both compressors by raising the set points to 1 C or switching off the instrument The waste bottle collects water from the cooling fins It also collects debris when the refrigerated chamber is washed down so the contents must be disposed of in an appropriate manner MAKE SURE ALL SURFACES AND PARTS ARE THOROUGHLY DRY BEFORE THE COMPRESSORS ARE RESTARTED s ALWAYS KEEP AT LEAST 200 ml OF 1096 FORMALIN SOLUTION 4 FORMALDEHYDE SOLUTION OR SIMILAR DE CONTAMINANT IN THE WASTE BOTTLE MAKE SURE THAT THE END OF THE DRAIN TUBE IS KEPT SUBMERGED s A78910100 Shandon Cryotome Operator Guide 71 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 71 e 2 1 2011 10 47 03 AM a SSE ee Cleaning and Maintenance Maintenance No maintenance is required to be carried out by the user If the fluorescent tube fails either follow the instructions below or call the Thermo Service Department SWITCH OFF THE INSTRUMENT AT THE MAINS AND REMOVE THE PLUG FROM THE WALL SOCKET BEFORE PROCEEDING WITH ANY MAINTENANCE s Removing the lamp tube Lift up the top cover DO NOT TOUCH ANY WIRES CONNECTORS OR COVERS INSIDE THE INSTRUMENT s Grip the fluorescent tube firmly rotate it be a quarter turn and pull it towards you to slide it from the end connectors Fitting the lamp tube Hold che replacement tube firmly at the ends and align the connector e pins with the slots at each end of
21. to or been in contact with potential pathogenic or radioactive material it is essential that it is decontaminated For decontamination reference should be made where appropriate to the European Directive 2000 54 EC To avoid any misunderstanding we request that all instruments or parts returned to us must be accompanied by a certificate stating the following has not been exposed to pathogenic radioactive or other hazardous material and has We certify that the instrument been cleaned has been exposed to pathogenic radioactive or other hazardous material and has been decontaminated and cleaned according to Serial No approved procedures following exposure to Has the instrument been used for work with human or animal Transmissible Spongiform Encephalopathies e g Creutzfeld Jacob disease Scrapie or BSE YES NO If YES please contact Thermo Service before taking any further action Name Block Capitals Full address Part2 GUIDELINES FOR RETURNING INSTRUMENTS Please use the checklist below left to ensure that the instrument being returned is ready for collection then fill in the details below right All reagents wax removed from instrument including vapour traps if applicable Instrument has had transit clamps fitted as detailed in the Operator Guide Instrument is packed in original packaging Thermo Fisher Scientific 93 96 Chadwick Road Astmoor Runcorn Cheshire WA7 1PR United Kingdo
22. where the required defrost program can be selected page bd Press FUMIGATE to display the fumigation screen page Unload specimen Unload c 1 FSE ONLY Specimen Unload 00 24 Note Use the UNLOAD SPECIMEN feature to warm the head to above 0 C to enable the cryocassette to be removed from the specimen head or the specimen from the cryocassette At the end of the unload or when CANCEL UNLOAD is pressed the specimen head will return to its set point S Either the UNLOAD SPECIMEN or the CANCEL UNLOAD button is displayed in the same screen location A78910100 Shandon Cryotome Operator Guide 41 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 41 e 2 1 2011 10 46 59 AM a SSE ee Controls Press UNLOAD SPECIMEN or press SPECIMEN on the main screen twice to start a specimen unloaded sequence The Main Screen will be displayed and will show that unloading is in progress the SPECIMEN button will have an asterix displayed and the text between the adjustment buttons will show the countdown time of 5 minutes Set SPECIMEN UNLD 00 24 Mim e To cancel the unload sequence and return to the Options Screen either press CANCEL UNLOAD or press the SPECIMEN button on the Main Screen when it is selected Cryobar boost Cruobar Cancel Cancel boost oos Bog io e Either the CRYOBAR BOOST or the CANCEL BOOST button is displayed in the same screen location To start a cryobar boost sequ
23. 100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 74 e 2 1 2011 10 47 03 AM Table 1 Instrument Function The Shandon Cryotome does not respond when the mains power is switched on The Shandon Cryotome is The instrument is still carrying out initial tests No mains supply The circuit breaker has tripped The mains fuses have blown Troubleshooting Wait approximately 30 seconds an hour glass is displayed for the initial tests to finish Connect the power lead switch on the mains power and instrument main power switch see pages Reset the circuit breaker by switch ing the instrument off and on again see page Replace the mains fuse Replace the instrument fuses Note only a technically competent person should replace fuses Switch off the instrument Wait for powered up but does not respond correctly The knife holder or any part The Shandon Cryotome may have started incorrectly 30 seconds Switch on the main power switch Full defrost Ice and or debris between the of the knife holder cannot be parts of the knife holder Remove debris clamped or unclamped A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 75 Shandon Cryotome Operator Guide 75 e 2 1 2011 10 47 03 AM HS Troubleshooting Table 2 Problems when sectioning Specimen cracks as it is frozen Too rapid freezing Reduce cryobar temperature Specimen too thick Cut specimen in
24. 100 Iss4 indb 100 e 2 1 2011 10 47 08 AM Es e 101 instrument function fault finding 75 sectioning fault finding 76 78 U unload specimen button 41 unlock button 41 security mode off 40 unpacking the instrument 11 up and down arrow buttons 38 W warnings before use 20 warning symbol A 4 warning symbol 4 warranty statement 86 waste bottle 21 weight 7 81 width 81 wiring convention 20 Shandon Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 101 e A78910100 2 1 2011 10 47 08 AM A78910100 Contact Addresses Anatomical Pathology Anatomical Pathology 93 96 Chadwick Road 4481 Campus Drive Astmoor Runcorn Kalamazoo Cheshire WA7 1PR UK Tel 44 0 1925 562600 Fax 44 0 1928 562627 www thermo com pathology WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 102 MI 49008 USA Tel 1 800 522 7270 Fax 1 269 372 2674 www thermo com pathology Shandon Cryotome Operator Guide e 2 1 2011 10 47 08 AM 102
25. Cryotome FE amp FSE Operator Guide English A78910100 Issue 4 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 1 e 2 1 2011 10 46 53 AM a SSE NENNEN Legal Information 2004 Thermo Fisher Scientific All rights reserved Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company Thermo Fisher Scientific is the trading name of Thermo Shandon Limited Isceon is a trademark of Rhodia Chimie Corporation All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its support documentation is correct and clearly stated but does not accept responsibility for any errors or omissions The development of Thermo products and services is continuous Make sure that any published information that you use for reference is up to date and relates to the status of the product If necessary check with Thermo or your local Thermo representative This manual may not in whole or in part be copied photocopied reproduced translated or converted into any electronic or machine readable form without prior written consent of Thermo Fisher All information contained in this manual is proprietary and confidential and the exclusive property of Thermo Fisher Scientific This manual is protected by copyright and any reproduction i
26. Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 97 anti roll plate 27 assembling 23 blade position clamp blue 23 fitting a disposable blade 24 position clamp black 22 setting 64 C centre of gravity 6 83 chamber button 37 cleaning 70 air intake 70 knife holder 70 knife holder support plate 70 cleaning and maintenance 67 defrosting 70 electrical protection 72 fumigation 68 maintenance 72 precautions 67 68 code screens 51 enter current code screen 52 enter new code screen 52 enter unlock code screen 52 re enter new code screen 52 contact addresses 2 101 cooling fins defrost button 54 Cryobar 42 cryobar boost cancel boost button 42 cryobar button 36 cryobar cover 31 cryobar defrost button 54 cryobar disable enable button cryobar boost cancel boost button cryocassettes A78910100 2 1 2011 10 47 08 AM WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 98 e cryocassette handle 31 cryocassette identifier 31 cutting specimens 64 cutting temperatures suggested 95 D Declaration of Conformity 87 defrosting 70 cooling fins defrost 54 cryobar defrost 54 defrosting screen 54 defrost button 41 defrost screen 55 defrost setup screen 54 defrosting screen 54 cancel defrost button 55 exit button 54 time display 53 depth 81 description of instrument 9 dimensions 11 81 display description 34 display setup screen 47 interactive sliders 56 select button 56 E
27. D OR REMOVE THE BLADE WHEN NOT CUTTING SECTIONS s FSE only To help remove the cassette and or specimen from the cassette either press SPECIMEN twice or go to the Options Screen and select UNLOAD SPECIMEN The specimen head will warm up to 2 c to allow the cassette and or specimen to be removed It will then return to it s set temperature Press SPECIMEN again to stop UNLOAD or CANCEL UNLOAD from the Options Screen page 40 The specimen set temperature will be replaced by UNLD during this period The display will flash and an audible warning given when the temperature reaches 2 C If the specimen is to be stored frozen protect the cut surface with Shandon Cryotome wrap the specimen in metal foil before putting it into a freezer Do not store it in the refrigerated chamber of the Shandon Cryotome If the specimen is to be fixed allow it to thaw before placing it in a suitable fixative otherwise discard it in accordance with local regulations and procedures Brush away any debris that has collected around the cassette clamp or head A78910100 Shandon Cryotome Operator Guide 65 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 65 e 2 1 2011 10 47 02 AM L ee Operation Decontaminate and clean the Cryocassette Note Do not use bleach sodium hypochlorite to clean the coloured cryocassettes unless this is a quick dip in bleach followed by a thorough water rinse prolonged contact with bleach will
28. L BOOST An audible warning will be sounded when the boost cycle is about to end s Turn the handle of the handwheel to the 12 o clock position and apply the break Fit the cryocassette handle supplied to the cryocassette and lock it in place by sliding the locking tab towards the cryocassette Transfer the Cryocassette complete with frozen specimen from the cryobar to the specimen head and secure it in position with the specimen clamp see page 29 The handle can now be removed if required A78910100 Shandon Cryotome Operator Guide 63 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 63 e 2 1 2011 10 47 02 AM a SSE ee Operation by sliding the locking tab away from the cryocassette Setting the Fit a cooled blade and or remove the blade guard blade holder If sufficient travel available is indicated on the Specimen Travel Indicator advance the specimen towards the knife using the advance push button of the control panel on the left hand side of the instrument Alternatively turn then handwheel to 3 o clock and lock it in position Pull the pivoting lever at the lower left hand side black of the knife holder towards you then carefully push the base of the knife holder towards you then carefully push the base of the knife holder along the carriage way from you until the blade is almost touching the specimen Lock the knife holder in position by pushing the pivoting lever away from you If neces
29. Note The day and time are always displayed at the top of the Main Screen S 46 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 46 e 2 1 2011 10 47 00 AM Es e Display Setup Screen Interactive Sliders Select Language Setup Screen A78910100 Display Setup aaa View m Controls The display setup screen consists of two interactive sliders for brightness and viewing angle and an EXIT button Note The brightness and view angle of the screen display may vary under different ambient conditions S Touch the screen at any point along the sliders to change the brightness and viewing angle of the screen The coloured bar will move to that point and the screen display will change Press SELECT to save the display setup and return to the Setup Screen page 44 Shandon Cryotome FSE Select Language poe ge ftware pangais 0026 Deutsch Espanol Italiano Portugu s Dansk y Svenska The Language Setup Screen shows the list of languages that can be selected Use the up and down arrows to select the required language for all the screen displays and then press SELECT to return to the Setup Screen page 44 Shandon Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 47 e 4 2 1 2011 10 47 00 AM a SSE ee Controls Security Setup Screen Security Mode Security Lock button loc
30. UTIONS s BE AWARE OF POTENTIAL BIOHAZARDS AND FORMALIN CONTAINED IN THE WASTE BOTTLE DISPOSE OF CORRECTLY IN ACCORDANCE WITH LOCAL PROCEDURES s BE AWARE THAT FORMALDEHYDE SOLUTION IS USED DURING FUMIGATION ALWAYS REFER TO MATERIAL SAFETY DATA SHEET MSDS s TO AVOID INJURY LOCK THE HANDWHEEL AND DO NOT MOVE THE SPECIMEN HEAD WHEN YOUR HANDS ARE IN THE CHAMBER s Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 60 e 2 1 2011 10 47 02 AM Es NENNEN Operation Touch the touch screen If no security is set the display will brighten and will be enabled If security is set press UNLOCK on the touch screen for 3 seconds or until an access code is requested This will also unlock the window and turn the chamber light on Note Refrigeration performance may be affected if the ambient temperature exceeds 25 C 77 F s Mounting a Use the cryobar to mount the specimen onto the cryocassettes as Specimen follows BE AWARE THAT THE CRYOBAR IS EXTREMELY COLD AND CAN CAUSE FROSTBITE DO NOT TOUCH IT WITH BARE HANDS WITH THE COVER REMOVED s Make sure the cryobar us free from frost and dirt Lower the heat extractor on to the cryobar position required and if the cryobar is not already being controlled at 55 C turn on the cryobar boost option from the Main Screen see page 36 by either pressing the CRYOBAR button twice or select OPTIONS and CRYOBAR BOOST Wh
31. Voltage Directive 2006 95 EC This product complies with the following International Standards EMC EN 61326 1998 EN 61000 3 2 2000 EN 61000 3 3 1995 Inc A1 2001 Safety EN 61010 2 101 2002 EN 61010 1 2001 CAN CSA C22 2 No 61010 1 04 UL Std No 61010 1 2 Edition Issued by K Waldron Quality Manager Thermo Fisher Scientific Anatomical Pathology Diagnostics Division LA COS res Date 1 June 2007 Optional accessories considered subject to the In Vitro Diagnostics Directive IVDD are specifically identified on this Declaration of Conformity Further supplies of standard accessories are treated as spares Convenience aids offered as accessories are not subject to the IVDD A78910100 Shandon Cryotome Operator Guide 88 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 88 e 2 1 2011 10 47 04 AM Es ee AppendixA Transportation Instructions If you ever need to transport the Shandon Cryotome follow these packaging instructions Repacking SHANDON CRYOTOME FE AND FSE WEIGH APPROXIMATELY 125kg 275lbs ALWAYS GET HELP TO SAFELY MOVE THE INSTRUMENT WITHOUT RISK OF INJURY s Follow the instructions in Chapter 6 and follow good laborartory practice to clean and decontaminate the instrument Complete and sign the Product Safety Declaration at the end of this chapter to confirm that the instrument is fully decontaminated Make sure that all knives blades and holders are removed from t
32. When cutting sections lower the anti roll plate carefully into position on the knife blade do not let it drop onto the blade Any damage to the edge of the glass could result in loss of performance Ensure that there is a good edge i e there are no nicks on the anti roll plate Use the brush supplied to keep the anti roll plate the blade and the clamp clean The anti roll plate glass has four usable edges If the edge being used is damaged loosen the two clamp screws pull the glass from its frame rotate it and re insert to present an undamaged edge for use Make sure the glass is pushed to the bottom of the holder and re clamp Note To prevent the anti roll plate heating up make sure that it is kept close to the blade holder s 28 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 28 e 2 1 2011 10 46 58 AM Es ee Installation and Setting Up Specimen Head The specimen heads are designed to accept a Shandon Cryocassette that already has a frozen specimen mounted The Shandon Cryotome FE can be fitted with either a fixed or a fine orientation head The Shandon Cryotome FSE is only fitted with a fine orientation head for use with independent specimen cooling The frozen specimen on the Cryocassette is held in the specimen head by a Quick Release Cryocassette Clamp The clamp lever at the left hand side of the Specimen head operates a moveable jaw at the bott
33. Window error lock Fumigation could not start The window could not be locked Fumigation Error There was a fault during fumigation THERE MAY BE FORMALDEHYDE FUMES INTHE CHAMBER s Troubleshooting Make sure that the specimen head is not ob structed Press EXIT to reset the microtome Remove the transit bracket Turn the instrument off and retry If neither of these solutions are successful call Thermo Service Turn off instrument and repack it to move it safely If the transit bracket has not been fitted clear the obstruction in front of the head and fully retract the specimen head Call Thermo Service Call Thermo Service Wait for the specimen temperature to stabalise in the chamber ambient before sectioning Make sure the window is fully closed and retry Make sure the window is fully closed and restart fumigation Call Thermo Service Ont Shandon Cryotome Operator Guide 79 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 79 e 2 1 2011 10 47 03 AM e Troubleshooting 80 Table 3 Error messages cont 9 System error A critical fault has occurred which may have resulted in one or more systems being disabled Specimen head ahas overheated The specimen head control has been disabled Read the text carefully Error 11 is either Cryobar refrigeration system fault or Chamber refrigeration system fault Specimen head temperature control fault Voltage Selection Error Fumigation
34. ained engineer in accordance with the instructions contained in the Shandon Cryotome Service Manual A78910101 16 Any problems and queries should be referred to your supplier 17 Refrigeration System The Shandon Cryotome uses Isceon 89 refrigerant THE REFRIGERATION SYSTEM MUST ONLY BE MAINTAINED BY SUITABLY QUALIFIED ENGINEERS s REFRIGERANTS ARE GREENHOUSE GASSES AND ALL PRECAUTIONS MUST BE TAKEN TO RECOVER ANY GAS REMOVED FROM UNITS AND TO PREVENT ITS RELEASE INTO THE ATMOSPHERE s ALWAYS USE THE CORRECT REFRIGERANT TO CHARGETHEINSTRUMENTANDALWAYS DISPOSE OF ANY USED REFRIGERANT IN ACCORDANCE WITH LOCAL AND NATIONAL REGULATIONS s 8 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 8 e 2 1 2011 10 46 54 AM a SSE NENNEN Chapter2 Description O The Cryotome is designed for cutting frozen sections of specimens verview med rapidly and accurately for future microscopic examination It operates by rapidly freezing the sample in a temperature controlled environment and provides a means for sectioning the frozen sample by conventional microtome The different components of the Cryotome are shown in the diagram below Touch screen display Main display includes the following items Micron selector Temperature controls Specimen travel indicator Rotary contrd Advance rewind controls Handwheel contains cryobar specimen head and knif
35. amber ress Exit to return to revious temperature settings A78910100 Shandon Cryotome Operator Guide 57 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 57 e 2 1 2011 10 47 01 AM a SSE NEM Controls IT IS IMPORTANT THAT ALL THE KNIFE HOLDER SECTIONS ARE SEPARATED WHEN CLEANING MAKE SURE THAT ALL SURFACES ARE COMPLETELY DRY BEFORE REASSEMBLING AND REFITTING INTO THE CHAMBER s Press EXIT to enable the instrument to return to normal operation with the previously set temperatures 58 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 58 e 2 1 2011 10 47 01 AM A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 59 Controls Shandon Cryotome Operator Guide 59 e 2 1 2011 10 47 01 AM Operation 60 Chapter 5 Operation When the instrument is not in use lock the Handwheel and keep the instrument switched on with the sliding window closed and locked and the refrigerated chamber at the normal operating temperature When the lock option is selected the following things occur e the touch screen control panel is disabled dependant on the security mode e the window is locked e the chamber light is turned off e the display is dimmed SURFACES INSIDETHE CHAMBERMAY BEEITHER VERY HOT OR VERY COLD AND MAY CAUSE INJURY IF TOUCHED WITH BARE HANDS s BE AWARE THAT TISSUE SAMPLES MAY POSE A BIOHAZARD TAKE SUITABLE PRECA
36. arge flap and teist the inner part of the plastic fixing so that the two cardboard flaps are joined together A78910100 Shandon Cryotome Operator Guide 92 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 92 e 2 1 2011 10 47 07 AM 7 Put the cardboard lid on top of the packed instrument and finally bind the complete package so that the bingings run over the lid and through the pallet 93 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 93 e 2 1 2011 10 47 08 AM PRODUCT SAFETY DECLARATION Part1 DECONTAMINATION CERTIFICATE Any instrument or part of any instrument must be clean before being returned or before any on site service and where necessary accompanied by a completed Decontamination Certificate If the instrument or any part of it is found to be in an unclean condition upon receipt or before a service or Thermo Fisher Scientific consider it to be a hazard the instrument or part will be returned unrepaired at the expense of the customer or service will be refused In the case of returns it is important that the certificate is forwarded by post or fax and a copy attached to the exterior of the container Containers will not be opened until the company is in possession of the required certificate This form MUST be completed by the customer and NOT a Thermo or distributor employee If the instrument or any part of it is to be returned to Thermo and has been exposed
37. arrow 1 A78930166 Wide A78930063 Linistat Stainer 220 240V 50Hz B1000201 110 120V 60Hz B1000200 REFER TO APPENDIX B FOR A LIST OF APPROVED REAGENTS IF A STAINING SYSTEM SUCH AS THE SHANDON LINISTAT IS USED ON TOP OF THE INSTRUMENT TM See catalogue for details of solid knives disposable blades Cryomatrix and other Cryostat accessories 84 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 84 e 2 1 2011 10 47 04 AM a SSE e Specifications and Accessories Spares Instrument Spares Quantity Part Number Allen key handwheel transit fixings 1 P13546 Anti roll plate glass 4 usable edges A78930200 Anti roll plate perspex 4 usable edges TBA Brush sections P12940 Brush debris P12257 Fluorescent tube AP14917 Mains leads UK P13291 EU P13290 US P13292 Operator Guide available in different languages A78910100 Spanner foot adjustment A78930178 A78910100 Shandon Cryotome Operator Guide 85 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 85 e 2 1 2011 10 47 04 AM a SSE ee Chapter9 Warranty Statement We are proud of our quality and reliability and of our after sales service We continuously strive to improve our service to our customers Please ask your distributor or representative about Service Contracts which can keep your purchase in peak condition for many years to come Warranty provisions necessarily vary to c
38. ck the handwheel in position The handwheel can be locked in one of three positions 12 o clock 3 o clock and 6 o clock by pushing the lever away from you A78910100 Shandon Cryotome Operator Guide 19 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 19 e 2 1 2011 10 46 56 AM e ee Installation and Setting Up Turn the handwheel until the handle is used to lock the handwheel in position then push the lever of the lock away from you Check that the lock operates and that the handwheel cannot rotate Check that the specimen head is at the top of its travel Move the lock lever towards you and check that the handwheel rotates smoothly and that the specimen head moves up and down Check also that the lock operates at the 3 o clock and 6 o clock positions Prepa ring the Read and understand the following warnings instrument for use PRACTICE GOOD BIOLOGICAL SAFETY PROCEDURES WHENEVER THE SHANDON CRYOTOME IS USED s MAKE SURE THAT THE WASTE BOTTLE IS FILLED WITH SUFFICIENT 10 FORMALIN SOLUTION TO COVER THE OUTLET PIPE REFER TO THE MATERIAL SAFETY DATA SHEET FOR FORMALIN AND FORMALDEHYDE s DO NOT USE THE SHANDON CRYOTOME FOR GENERAL LABORATORY REFRIGERATION PURPOSES s Fumigate regularly to ensure decontamination of the refrigeration chamber and accessories s WARNING Lll m WARNING Use cut resistant gloves when handling knives and blades s MZ NSININIE Use the correct type of knife or blade a
39. cked Specimen is too cold when cut Specimen not frozen quickly enough Specimen too thick Frost or dirt on cryobar Knife not supported correctly Knife angle incorrect Specimen not sufficiently secured The blade is not clamped securely Remove clean the knife Check and if necessary sharpen the knife or use a new knife Inspect and if necessary replace the anti roll plate or rotate the plate to a new edge there are 4 usable edges Usually applies to fixed tissue Soak in Dextran before freezing Allow Cryoboost longer time before adding mountant and admitting specimen Cut specimen into thinner block Use cryobar cover to stop frost forming or debris collecting on the cryobar Disposable knives are most vulnerable Make sure that the knife is fitted cor rectly and that there is no debris or ice under knife clamp Check and correct the knife angle Refreeze as necessary Make sure the blade clamping is secure Blade clamping force can be increased by tightening the clamp screw at the back of the top stage ont Shandon Cryotome Operator Guide 77 e 2 1 2011 10 47 03 AM Troubleshooting Table 2 Problems when sectioning cont Sections thick and thin Sections stick to the anti roll plate Sections are split vertically Sections curl after lifting from anti roll plate Blunt knife or blade Knife angle too shallow Specimen not secure Knife holder is loose Specimen temperature i
40. code press the highlighted CHANGE ACCESS CODE button and press SELECT to confirm the selection The Enter Current Code Screen will be displayed page 52 Input the current access code and press ENTER Confirm this code when the Re enter New Code Screen is displayed and press ENTER to return to the Setup Screen page 44 If the instrument is in the security off or hold key modes ie the SECURITY OFF button or HOLD KEY TO UNLOCK button is highlighted choose the access code mode by pressing the ACCESS 50 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 50 e 2 1 2011 10 47 00 AM Es NEM Controls CODE button Press SELECT to confirm the selection The Enter New Code Screen will be displayed page 52 Input the new access code and press ENTER The Re enter New Code Screen will be displayed page 52 Input the new access code and press ENTER to return to the Setup Screen page 44 Access code screens Enter New Code HHHH E SHW P This selection applies to a series of four screens that all look similar The screen names are displayed at the top of the screen in this case Enter New Code The four screen are Enter New Code Re enter New Code Enter Unlock Code Enter Current Code The buttons on all the Access Code Screens are described below Number Buttons 1 Press the relevant number button to input the requires Access
41. ction of the knife holder so that the base section plunger locates in the middle section slot Line up the two sections and push the red angle clamp to lock them in position Assembling the disposable blade holder Pull the blue blade position clamp towards you Line up the dovetails on the top section and the middle section and slide the two parts together When the top section is central push the blue blade position clamp away from you to tighten it Note When fitting the top section to the middle section lift the end of the top section slightly when sliding it along the middle section s A78910100 Shandon Cryotome Operator Guide 23 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 23 e 2 1 2011 10 46 57 AM Installation and Setting Up Slot Top section Angle clamp RED Plunger Base section Position clamp BLACK Blade position clamp BLUE Middle section The blade holder is now assembled Note Make sure that all the assembly slots dovetails and plungers are free from debris If debris is present the assembled blade holder may not provide the required stability s Fitting the disposable Once assembled the disposable blade holder can be fitted into the blade holder Shandon Cryotome FE and FSE chamber ALWAYS LOCK THE HANDWHEEL TO PREVENT THESPECIMEN HEAD MOVING WHEN WORKING NEAR THE KNIFE HOLDER s Pull the black position clamp on the left hand side of the base section fully toward you Insert the pl
42. d out when absolutely necessary as damage to sensitive electronic devices may occur It must only be carried out by a competent person s WARNING ie WARNING Internal fuses are used to protect the electronics within the instrument The fuses should only be changed by a qualified service engineer S A78910100 Shandon Cryotome Operator Guide 73 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 73 e 2 1 2011 10 47 03 AM a SSE ee Troubleshooting Chapter7 Troubleshooting Correct service and maintenance is essential for the long term serviceability of precision engineered products such as the Shandon Cryotome We strongly recommend that a Thermo Service Contract is used to ensure future reliability and consistency of performance Shandon Cryotome has self test routines built in so that error codes show in the display if malfunctions occur You will be advised whether you need to contact your Thermo Engineer for service Table 1 lists basic instrument function problems and solutions Table 2 lists other problems mainly concerned with sectioning and their causes that are not shown in the displays Table 3 lists alarm signals their causes and the remedial action to be taken If an error indication continues or recurs remove the samples from the refrigerated chamber and store them in a suitable refrigerated container and contact your Thermo Service Representative 74 Shandon Cryotome Operator Guide A78910
43. des ALWAYS USE CUT RESISTANT GLOVES TO AVOID INJURY WHEN HANDLING DISPOSABLE BLADES USE THE DISPENSER WHEN FITTING A NEW BLADE s Use the anti roll plate handle to swing the anti roll plate away from the blade clamp Pull the black blade clamp lever towards you to release the blade clamp Push the end of the blade to be installed part way out of the dispenser and feed it behind either end of the blade clamp Slide the blade to the right until it is locked symmetrically in the top stage behind the blade clamp Note The blade must be located symmetrically for correct operation of the anti roll plate s Blade fitted behind blade clamp Anti roll AN plate Anti roll plae ZN handb Blade cl mp lever BLACK 26 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 26 e 2 1 2011 10 46 57 AM Es ee Installation and Setting Up Push the black blade clamp lever back to clamp the blade in position and gently swing the anti roll plate on top of the blade Anti roll plate Anti roll plae handle Blade clamp lever BLACK USE THE CORRECT BLADE TYPE FOR THE BLADE HOLDER EITHER LOW OR HIGH PROFILE s FIT THE KNIFE GUARD OR REMOVE THE BLADE WHEN NOT CUTTING SECTIONS s The top section of the blade holder can be moved to allow the entire length of the blade to be used Pull the blue blade position clamp towards you to loosen the top section Slide the to
44. don Cryotome can be moved for short distances less than 2 metres 6ft without tilting the instrument for example to pull the instrument away from the wall To do this use the spanner provided to turn the two front feet clockwise until the two front feet of the instrument are clear of the floor Push the instrument When in position turn the front feet e counter clockwise to lower them to the floor and adjust to level the instrument Note The feet can be lefi in the raised position but there may be a slight movement when sectioning S THE SHANDON CRYOTOME IS HEAVY TO AVOID COLLISIONS ALWAYS MAKE SURE THE ROUTE IS CLEAR WHEN MOVING THE INSTRUMENT s A78910100 Shandon Cryotome Operator Guide 13 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 13 e 2 1 2011 10 46 55 AM Installation and Setting Up 14 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 14 e 2 1 2011 10 46 55 AM Installation Move the instrument to its permanent location This must be level and provide a 300 mm 12 clearance to the sides and rear of the instrument for ventilation Note The transport handles at the rear of the instrument can be left in place S Note Performance of the refrigeration system may be affected at ambient temperatures above 25 C 77 F s Note Siting of the instrument on an uneven surface can have an adverse effect on the cooling
45. e are any breakages or shortages Quote the instrument serial number located underneath the top cover at the front of the instrument on the right hand side your order number delivery note or packing slip number and the date s Note Jf you ever need to transport the instrument refer to Appendix A for repacking instructions S Always keep the instrument as near to vertical as possible to WARNING prevent damage to the refrigeration system s Consult your Thermo dealer if the Shandon Cryotome is to be WARNING moved to another building or out of the area The transit fixing must be re fitted The instrument must be kept upright at all times SHANDON CRYOTOME FE AND FSE WEIGH APPROXIMATELY 125KG 275LBS ALWAYS GET HELP TO SAFELY MOVE THE INSTRUMENT WITHOUT RISK OF INJURY s When the Shandon Cryotome has to be moved for more than 2 metres 6ft use the handles to the back of the unit to tilt the instrument backwards slightly to wheel it like a trolley DO NOT LET THE INSTRUMENT DROP ONTO THE FRONT ROLLERS s To avoid damage to the compressor and to prevent power trips if the instrument has been transported or tilted backwards 12 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 12 e 2 1 2011 10 46 55 AM a SSE ee Installation and Setting Up beyond 45 let it stand for 24 hours minimum before switching WARNING on to allow compressor oil to settle s The Shan
46. eholder Foot rest A78910100 Cryotome Operator Guide 9 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 9 e 2 1 2011 10 46 54 AM Es ee For optimum performance the microtome specimen head and knife holder are held within the refrigerated chamber The main body of the microtome is mounted outside the chamber to give more space for working in and for easier cleaning Principle factors for good sectioning of frozen specimens are 1 the temperature must be correct for the specimen being cut 2 the microtome must be correctly adjusted and operated 3 the cutting blade must be sharp and set at the correct angle 4 the anti roll plate must be correctly adjusted 10 Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 10 e 2 1 2011 10 46 54 AM a SSE ee Installation and Setting Up Chapter3 Installation and Setting Up The Shandon Cryotome is a precision instrument that must be unpacked and installed with care The maximum overall dimensions of the Shandon Cryotome FE and FSE cryostats are Width with handle 830 mm 33ins Depth 745mm 29 ins Height working height 1050mm 41 ins instrument height 1205 mm 47 2 ins SHANDON CRYOTOME FE AND FSE WEIGH APPROXIMATELY 125KG 275 LBS ALWAYS GET HELP TO SAFELY MOVE THE INSTRUMENT WITHOUT RISK OF INJURY s If the packaging has
47. en in the required position e Position adjust clamp lever Pull to release push to clamp Vertical adjustment knob Clockwise to tilt specimen upwards Counter clockwise to tilt specimen downwards Horizontal adjustment knob Clockwise to tilt specimen to the left Counter clockwise to tilt specimen to the right Quick release cryocassette clamp lever Pull to release push to clamp Note The picture shows the head for the FE The FSE head has controls in similar positions S 30 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 30 e 2 1 2011 10 46 58 AM a SSE NEM Installation and Setting Up Accessories The following accessories can be used with the Shandon Cryotome and FE and FSE Shelves Fit the narrow shelf with side lip to the right hand side of the chamber against the side wall if it is required for tool storage Fit the flat shelf next to the cryobar for storage of specimens at chamber temperature Cryocassettes A range of cryocassette sizes and styles are available Cryocassette handle A removable lockable handle can be used to handle the cryocassettes Cryocassette identifier These are clear strips with numbered rings designed to fit on the cryobar or shelves to locate and identify cryocassettes that are being frozen or stored before sectioning Heat extractors The heat extractors fit in the slots beside the cryobar to make specime
48. en the boost option is selected the CRYOBAR button on the main screen will display an and the time remaining for the boost will be displayed between the adjuster control arrows The time can be adjusted by using Li or S CRYOBAR ene edes The cryobar temperature is displayed on the Main Screen In the illustration above the actual cryobar temperature is 55 C A78910100 Shandon Cryotome Operator Guide 61 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 61 e 2 1 2011 10 47 02 AM e NENNEN Operation THE CRYOBAR IS EXTREMELY COLD AND CAN CAUSE FROSTBITE DO NOT TOUCH IT WITH BARE HANDS s FSE version only Use the table in Appendix C as a guide to select the temperature for the specimen to be cut according to the tissue type FSE version only To set the specimen head temperature press SPECIMEN to select it and then use or to set the required temperature FSE version and FSE versions Press CHAMBER and use Or to set the chamber temperature to the required temperature normally between 20 C and 25 C but should be at least as low as the specimen temperature guidelines listed in Appendix C Make sure you have a cooled blade ready for use There are several methods of embedding a specimen for work on a Shandon Cryotome The following instructions describe one suggested method Take a clean Cryocassette that has been held a room temperature Apply a layer of specimen embeddin
49. ence either press CRYOBAR BOOST or press the CRYOBAR button on the Main Screen when it is already selected The Main Screen will be displayed and will show that the boost sequence is active the CRYOBAR button will have an asterix displayed and the text between the adjustment buttons will show the amount of time remaining for the boost cycle Set CRYOBAR 23 36 E 55 c Press CANCEL BOOST in the Options Screen to stop the boosts cycle and return to the Main Screen 42 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 42 e 2 1 2011 10 46 59 AM a SSE NEM Controls Note Use the Cyrobar Boost to force the cryobar to go to its lowest temperature lt 55 C The Cryobar Boost lasts for 25 minutes and then returns to its setpoint or off S Cryobar disable Cryobar Cryobar Cryobar enable Disable Enable Either the CRYOBAR DISABLE or the CRYOBAR ENABLE button is displayed in the same screen location If the cryobar is enabled press CRYOBAR DISABLE When the cryobar is disabled its compressor is switched off and it will be cooled by the chamber temperature and will not be cooled to a lower temperature Note When the cryobar is selected on the Main Screen while it is disabled OFF will be displayed between the Adjuster Control arrows in the main screen S If the Cryobar is enabled it can be disabled by l Press on the Main Menu until the temperature
50. ess EXIT to leave the Setup Screen and return to the Main Screen page 36 Exit Set Clock Screen Set Time and day display The time and day display shows the days of the week and the time in either 12 hour or 24 hour format The and buttons above and below each part of the Time and Day display will increase or decrease that value by 1 for example E utton above the day Wednesday Thursday Friday Saturday E utton below t 4 button above t e day Monday Sunday Saturday he hour Increments the hour display by 1 hour including changing the am Shandon pm setting if 12 hour format is selected 05 06 07 E utton below the hour Decrements the hour display by 1 hour 03 02 01 E s utton above the minute Increments the minute display by 1 minute 13 14 15 E utton above the minute Decrements the minute display by 1 minute 11 10 09 A78910100 Shandon Cryotome Operator Guide 45 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 45 e 2 1 2011 10 47 00 AM a SSE ee Controls 12 Hour Either the J2HOUR or the 24HOUR button is displayed in the same screen location The 24HOUR button is only displayed when the clock is in 12 hour mode In 12 hour mode the display will show am or Shandon Shandon pm Press 24HOUR to change the time display to 24 hour mode and vice versa Set et Press SET to exit to the Setup Screen and save changes made to the time and day
51. etup t Exit Set time for daily defrost Use this screen to set the start item for the daily automatic defrost cycle which only defrosts the cooling fins in the back of the chamber The buttons on the Defrost Setup Screen are described below Note If the clock is set to 24 hour mode then the defrost time will also be in 24 hour mode if the clock is set to 12 hour mode then the defrost time will also be in 12 hour mode s 52 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 52 e 2 1 2011 10 47 01 AM a SSE ee Controls Time Display Increment Button 23 30 Decrement Button The main part of the Defrost Setup Screen is taken up with the INCREMENT and DECREMENT buttons and the Time Display The time Display shows the defrost start time in hours and minutes The fft and buttons above and below each part of the Time Display will increase or decrease that value by 1 for example L3 button above the hour ncrements the hour display by 1 hour Note When the hour display reaches maximum the hour display will revert to 0 button below the hour Decrements the hour display by 1 hour L3 button above the minute ncrements the minute display by 1 minute Note When the minutes display reaches 60 the hour display will NOT increment button above the minute Decrements the minute display by 1 minute Set Set Press SET to confirm the defro
52. g medium such as Cryomatrix or Cryochrome to the grooved side of the Cryocassette and place the Cryocassette on the cryobar When the embedding medium starts to freeze place the specimen directly from the cutting up board onto the embedding medium and press down lightly to eliminate any entrapped air Add extra mountant to support the specimen if required To give a flat and parallel surface and quicker freezing lower the cooled heat sink onto the specimen Allow the specimen and mountant to freeze until both are opaque and firm 62 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 62 e 2 1 2011 10 47 02 AM Es ee Operation TO AVOID THE POSSIBILITY OF CROSS CONTAMINATION 1 MAKE SURE THAT THE CRYOBAR AND HEAT EXTRACTOR ARE COLD ENOUGH 2 DO NOT USE TOO MUCH PRESSURE WHEN APPLYING THE HEAT EXTRACTOR 3 MAKE SURE THE CRYOCASSETTES AND HEAT SINKS ARE CLEAN BEFORE USE s Note Do not use excessive mountant or get it onto the sides or back of the Cryocassette S Note CRYOBAR BOOST continues for 25 minutes after cryobar boost is initially enabled This time can be adjusted in 5 minute steps by pressing or There are two ways to switch off the cryobar boost during this period 1 Press 8 until the time displayed between the Adjuster Control Arrows is 15 seconds The instrument will then count down until the boost ends 2 Select OPTIONS followed by CANCE
53. he instrument Remove the shelves heat extractors and tools from the chamber Use the Advance Control Panel to position the Specimen Head in the fully retracted posiiton Move the specimen head to the top of its travel Fit the transit bracket to the knife holder support plate and slide it to the back of thechamber Lower the specimen head into the bracket Place the face plate at the rear of the specimen head into the transit bracket clamp and tighten the cap screw on the base of the transit bracket knob Gradually tighten the other two cap screws MAKESURETHE CAP SCREWS ARETIGHT BEFORE MOVING ON TO THE NEXT STAGE s 89 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 89 e 2 1 2011 10 47 04 AM Specimen head face plate Transit bracket cap screws Transit bracket Use the control panel to move the specimen head forward until it jams The screen will display an Error 2 warning screen Switch off the instrument Do not acknowledge the error Switch off the instrument and remove mains cables from the rear of the elctronics box Empty the waste bottle and replace it in the instrument BE AWARE OF POTENTIAL BIOHAZARDS AND FORMALIN CONTAINED IN THE WASTE BOTTLE DISPOSE OF CORRECTLY IN ACCORDANCE WITH LOCAL PROCEDURES s Remove the handwheel bolt and remove the handwheel Lift the top cover and secure the window in the closed position using sticky tape Turn the instrument lo
54. is a CANCEL DEFROST button described below Cancel Defrost cancel Defrost Press CANCEL DEFROST to cancel the defrost and return to the Main Screen page 36 Note A cryobar defrost will not affect the chamber temperature S Note During the cooling fin defrost cycle the chamber temperature will rise However the cryobar will not be affected if it is enabled s THE CRYOBAR MAY REACH TEMPERATURES OF MORE THAN 50 C DURING THE DEFROST CYCLE 1 REMOVETHE SPECIMENS FROM THE CRYOBAR BEFORE DEFROSTING 2 DO NOT TOUCH THE CRYOBAR DURING DEFROSTING s Fumigating Screen To start the fumigation cycle press FUMIGATE on the Options Screen page 40 Instructions will be shown on the screen Press START to begin the fumigation A78910100 Shandon Cryotome Operator Guide 55 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 55 e 2 1 2011 10 47 01 AM a SSE ee Controls FUMIGATE Load the heatin trou LR in the refrigerated cham with 3ml of Formaldehyde solution Close the window then press STA If the window is not closed or cannot be locked the fumigation program will not start and will cause an error An audible alarm will be sounded and the Main Screen will display the error page 36 The fumigation cycle is as follows l The instrument performs a defrost 2 The chamber is warmed up 3 The formaldehyde solution is heated for 30 minutes and the instrument fumigates for 4 hours
55. ks Off the window and prevents settings rom being R Modified Hold Rey to iUnlock Access Exit Code The buttons on the Security Setup Screen are used to select the relevant security mode The security mode determines how you access the instrument when it has been locked The highlighted button shows the current security mode Security Mode The security mode is activated when the LOCK key in the options Activation screen is pressed page Note The EXIT button will change to SELECT if you press any of the security mode buttons S e Security Modes The three different security modes are described below Security Off When the security mode is activated the window will be locked and the controls disabled The screen options remain active To release the controls unlock the screen from the Options Screen Hold key to unlock This is the default option It provides basic security to prevent inadvertent button presses for example when cleaning the screen When the hold key mode is activated the window will be locked and the screen options and controls disabled When the screen or a control is touched or moved the TOUCH AND HOLD TO UNLOCK button is displayed Touch and Hold to Unlock Press and hold this button for approximately 3 seconds to unlock the instrument 48 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 48 e 2 1 2011 10 47 00 AM Es ee Contro
56. ls Access Code This provides full security where an access code number is required to unlock the instrument When the access code mode is activated the window will be locked and the screen options and controls disabled When then screen or a control is touched or moved the Enter Unlock Code Screen is displayed page 52 The current access code must be entered before the screen or controls can be used Security Mode The section describes how to select each security mode To access Selection the Security Mode Screen from the Main Screen select OPTIONS SETUP and SECURITY SETUP The current security mode will be highlighted Security Off s To set the security off mode If the instrument is already in the security off mode ie the SECURITY OFF button is highlighted the selection can be confirmed by pressing the SECURITY OFF button Press SELECT to return to the Setup Screen page 44 If the instrument is in the hold key mode ie the ZOLD KEY TO UNLOCK button is highlighted the selection can be confirmed by pressing the SECURITY OFF button Press SELECT to return to the Setup Screen page 44 If the instrument is in the access code mode ie the ACCESS CODE button is highlighted choose the security off mode by pressing SECURITY OFF button Press SELECT to confirm this selection The Enter Current Code Screen will be displayed page Input the current access code and press ENTER to return to the Setup Screen page 44
57. m Tel 44 0 1928 566611 Fax 44 0 1928 565845 www thermo com shandon 784 1505 issue 2 110 Shandon Cryotome Operator Guide A78910100 Issue A78910100 Shandon Cryotome Operator Guide 94 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 94 e 2 1 2011 10 47 08 AM e ee AppendixB Approved Reagent List This Section lists all the reagents that Thermo specify can be used with the Shandon Cryotome Cryostat If you want to use a reagent not included in this list contact your Thermo agent for advice ALWAYS REFER TO THE MATERIAL SAFETY DATA SHEET MSDS FOR THE REAGENTS USED s Reagent List Industrial Methylated Spirits IMS reagent alcohol up to 5 methanol in ethanol Isopropyl Alcohol IPA Formaldehyde solution Formalin solution Linistat staining reagents Xylene Toluene Shandon Xylene Substitute Histosolve Ethanol Haematoxylin Eosin aqueous and alcohol based EA50 65 OG6 Scotts Bluing Reagent Phosphotungstic acid 1 in water Industrial Methylated Spirits IMS reagent alcohol 9up to 5 methanol in ethanol Cleaning Reagents Dilute Detergent Sodium Hypochlorite 10 not for coloured cryocassettes Water WARNING Do not use ammonia or acidic solutions inside the chamber 95 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 95 e 2 1 2011 10 47 08 AM a SSE ee AppendixC Examples This Section provides
58. move the shelves debris tray covers knife holder and any debris Wash the shelves debris tray and covers in running hot water initially to remove the debris Wash dry thoroughly and replace in the Shandon Cryotome Follow the instructions below to clean the knife holder If the chamber is cold wash it with alcohol to remove debris and make sure the outlet tube in the bottom of the chamber is free from debris Do not use scouring powder acidic solutions or harsh detergents ZENNI or you will degrade the brushed finish s Press EXIT and the chamber will return to the previously set temperature A78910100 Shandon Cryotome Operator Guide 69 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 69 e 2 1 2011 10 47 03 AM a SSE ee Cleaning and Maintenance Cleaning Use a spray foam cleanser or a cloth damped in soapy water and a dry polishing cloth to periodically clean the outside of the cabinet DO NOT USE EXCESSIVE WATER Knife Holder It is important to keep the knife holder clean Remove the knife or blade before cleaning knife holder s Remove any build up of debris with a stiff brush If necessary soak the brush in alcohol or use a tissue soaked in alcohol IT IS IMPORTANT THAT ALL THE KNIFE HOLDER SECTIONS ARE SEPARATED WHEN CLEANING MAKE SURE THAT ALL SURFACES ARE COMPLETELY DRY BEFORE REASSEMBLING AND REFITTING INTO THE CHAMBER s Knife Holder Support Plate Remove any debris ice o
59. n Cryotome Operator Guide 81 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 81 e 2 1 2011 10 47 03 AM a SSE ee Specification and Accessories Fuses Fuses should only be replaced by technically competent personnel Main plug fuse 13A 250V where applicable Main fuses x 2 F5A 250V Part number P11171 Main fuses must only be replaced by service personnel Remote alarm fuse F5A 250V Part number P11171 G1 Switch Convention I Power On O Power Off Environment General Indoor use only Safe operating 5 C to 40 C temperature Recommended operating 45 C to 35 C temperature NOTE refrigeration performance may be degraded at ambient temperatures above 25 C s Transit storage temp 25 C to 55 C 70 C for short exposure Humidity 80 max for temperatures lt 31 C 50 max for temperatures 31 C to 40 C Non condensing environment Altitude Up to 2000m 6 500 feet Pollution degree 2 Over voltage category II 82 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 82 e 2 1 2011 10 47 03 AM a SSE ee Specifications and Accessories Part Numbers Shandon Crytome FE 230V 50Hz 478900001 115V 60Hz 478900002 Shandon Cryotome FSE 230V 50Hz A78900003 115V 60Hz A78900004 Centre of Gravity See also page Microtome General Rotary rocking microtome mounted outside refrigerated chamber To
60. n embedding and preparation easier Three different weights are available Cryobar Cover If the cryobar is at a lower temperature than the chamber it will attract frost To keep the cryobar surface clear from frost and debris place the cryobar cover on it whenever it is not being used to freeze specimens Footrest To adjust the foot rest to suit individual requirements unscrew and remove the knobs that secure the footrest to the instrument DO NOT STAND ON THE FOOT REST s Height positions Foot rest knobs A78910100 Shandon Cryotome Operator Guide 31 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 31 e 2 1 2011 10 46 58 AM a SSE ee Installation and Setting Up Move the foot rest to one of the other positions and line up the footrest with the appropriate hole Insert the knob and tighten it slightly to allow you to attach the other side of the foot rest Tighten both knobs Note The footrest does not have to be fitted Remove it if this is more convenient S Remote Alarm A switch is provided for connection to a remote alarm circuit The switch operates if i A power fail occurs ii An error occurs from which the instrument cannot automatically recover iii The instrument malfunctions Installation and Setting Up Remote alarm relay connections NO C NC Remote alarm fuse
61. ncorrect Dirt or contaminant on the anti roll plate Anti roll plate edge is damaged Cutting equipment not cool enough The knife is dirty or blunt The knife blade anti roll plate or clamp plate are damaged Anti roll plate too warm Blade blunt Static electricity in chamber 78 Shandon Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 78 e Sharpen knife or use new blade Widen the knife angle Re freeze and secure specimen Make sure the knife holder is secure on the base Raise or lower the temperature Use the brush to clean the anti roll plate Change to new edge on anti roll plate Allow more time for the knife and the anti roll plate to cool Remove the knife clean and sharpen it WEAR CUT RESISTANT GLOVES Examine the knife blade anti roll plate and clamp plate and replace if necessary Lower chamber temperature Change blade A78910100 2 1 2011 10 47 03 AM e Table 3 Error messages Help Screens are displayed for each error message 1 A78910100 Microtome fault A problem has occurred when the software has tried to initialise the microtome Transit bracket has been fitted success fully Chamber temperature sensor is faulty The chamber will be driven at maximum cooling effect Cryobar Temperature Sensor is Faulty The cryobar will be driven at maximum cooling effect Specimen temperature sensor is faulty The specimen head control has been disabled
62. nspected for obvious damage whenever it is cleaned If any damage is found contact your Thermo supplier Routine maintenance by the operator does not require the removal of any panels or fixtures Precision maintenance and adjustment should only be performed by Thermo trained service personnel and it is recommended that a Maintenance Contract is obtained from our Service Department IF HAZARDOUS MATERIAL IS SPLIT ON OR INSIDE THE INSTRUMENT THE USER SHOULD CARRY OUT THE APPROPRIATE DECONTAMINATION see World Health Organisation Laboratory Biosafety Manual s CLEANING OR DECONTAMINATION METHODS OTHER THAN THOSE RECOMMENDED IN THE OPERATOR GUIDE SHOULD BE CHECKED WITH A THERMO AGENT TO ENSURE THAT THE PROPOSED METHOD WILL NOT DAMAGE THE EQUIPMENT s A78910100 Shandon Cryotome Operator Guide 67 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 67 e 2 1 2011 10 47 02 AM e ee Cleaning and Maintenance ALWAYS REFER TO MATERIAL SAFETY DATA SHEET MSDS FOR REAGENTS SPECIFIED IN APPENDIX B s ALWAYS WIPE UP ANY SPILLS IMMEDIATELY IN THE EVENT OF A MAJOR SPILLAGE DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY WITHOUT DELAY AND DO NOT RECONNECT AND SWITCH ON UNTIL THE INSTRUMENT HAS BEEN THOROUGHLY DRIED OUT AND CHECKED BY A SERVICE ENGINEER s POTENTIALLY LETHAL VOLTAGES ABOVE 110V ac ARE PRESENT INSIDE THE UNIT DO NOT REMOVE ANY ACCESS COVERS s Always wear protective gloves when you clean
63. om of the head that holds the Cryocassette firmly in position To install a cryocassette in the Quick Release Clamp i Pull the left hand clamp lever forward ii Fit the cryocassette under the two fixed jaws as shown iii Push the clamp lever back Note The cryocassette handle supplied may be fitted to the cryocassette to aid manipulation and fitting to the specimen head s Fixed head FE Fine orientation head FE Fixed jaws Fixed jaws Quick release cryocassette clamp lever Pull to release push to clamp shown in released positio A78910100 Shandon Cryotome Operator Guide 29 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 29 e 2 1 2011 10 46 58 AM a SSE ee Installation and Setting Up Fine Orientation To adjust the up to down and left to right orientation of the Head specimen before sectioning takes place pull the right hand clamp lever towards you to unlock the clamp Use the knurled rotary control at the top of the orientation head to set the verticle orientation of the specimen Clockwise rotation tilts the specimen upward counter clockwise rotation tilts the specimen downward Use the knurled rotary control at the left hand side of the orientation head to set the horizontal orientation of the specimen Clockwise rotation tilts the specimen to the left counter clockwise rotation tilts the specimen to the right Push the clamp lever on the right hand side away from you to lock the specim
64. omply with differences in national and regional legislation and you can find details in your delivery documents or from your dealer or representative Please note that your warranty may be invalid i e the instrument is modified in any way accessories and reagents are used that are not approved by Thermo or e the instrument is not operated or maintained in accordance with the instructions in this Operator Guide 87 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 87 e 2 1 2011 10 47 04 AM Es ee Declaration of Conformity This Declaration of Conformity is only valid when the instrument is used in accordance with the Operator Guide A78910100 Manufacturer s Name Thermo Shandon Limited Trading as Thermo Fisher Scientific Manufacturer s Address Chadwick Road Astmoor Runcorn Cheshire WA7 1PR ENGLAND Product Description Cryostat Product Designation Shandon Cryotome FE Shandon Cryotome FSE Part numbers A78900001 A78900002 A78900003 A78900004 including accessories supplied as standard and the following accessories Orientation Heads 475910016 A78910022 Cryocassetles 77230540 77230546 A78910031 A78910055 A78910056 A78910057 A78910083 Knife Holders A78910032 A78910033 Year of Marking CE 2005 This product conforms with the essential requirements of the following directives In Vitro Diagnostics Directive 98 79 EC Low
65. or decontaminate the Shandon Cryotome to protect yourself against the effects of chemicals s WARNING Do not use any chemicals that may interact with materials of manufacture see Appendix B If in doubt check with the Thermo Service Department s WARNING Do not use the abrasive compounds or metal components to clean the Shandon Cryotome or its components and accessories s lli pe WARNING Fumigation The fumigation cycle is as follows 1 The instrument performs a defrost 2 The chamber is warmed up 3 The formaldehyde solution is heated for 30 minutes and the instrument fumigates for 4 hours 4 Finally fumes and excess moisture are extracted from the chamber 68 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 68 e 2 1 2011 10 47 02 AM Es ee Cleaning and Maintenance Carefully load the heating trough in the refrigerated chamber with 3ml of formaldehyde solution Close the window and press OPTIONS To start the fumigation press FUMIGATE in the Options Screen The Fumigation Screen will display instructions Press START to begin the fumigation cycle If the window is not closed the screen will show an error and will prompt you to close it VANNE Do not use any ammonia based reagent to neutralise formaldehyde s Note For safety the fumigation cycle cannot take place if the window is not closed and locked s End of fumigation Carefully re
66. ore than 3 seconds the specimen head will accelerate to its maximum speed To stop rapid retraction release the retract button then press either advance or retract button to stop To stop rapid advance of the specimen head release the advance button 5 Note There is no audible beep when the advance or retract buttons are pressed Used for fine control advance and retract Rotary Control Turn the rotary control clockwise to advance the specimen towards the knife Turn the rotary control anti clockwise to retract the specimen away from the knife Note Each notch of the control knob gives 8um of movement s A78910100 Shandon Cryotome Operator Guide 35 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 35 e 2 1 2011 10 46 59 AM a SSE e Controls Screen Controls The Shandon Cryotome is controlled by pressing buttons on the touch screen display panel This section describes each screen and the buttons that appear Main Screen Set Tuesday 04 12 7 Selector Buttons IMI CRONS CRYOBAR bpm 59c e CHAMBER SPECIMEN 20 c 20 c The buttons are described below MICRONS hym This displays the selected section thickness in um Normally when the Main Screen is entered the MICRONS button will be highlighted 55 c This displays the actual temperature of the cryobar in C Microns Cryobar 36 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100
67. otome FSE OP GUIDE A78910100 Iss4 indb 4 e 2 1 2011 10 46 53 AM Es ee Welcome Chapter 1 Welcome Welcome to Thermo Fisher Scientific Shandon Cryotome FE and FSE Cryostats They are designed for the routine sectioning of quick frozen tissue specimens for subsequent preparation and diagnosis by a pathologist The Shandon Cryotome series of cryostats are intended for use in pathology laboratories by appropriately trained medical laboratory technicians Designed and made with care the instrument is safe to use simple to operate and easy to maintain The Shandon Cryotome series of cryostats conforms with IEC61010 1 and EN61326 the safety and EMC standards for laboratory instruments This Operator Guide gives instructions for the correct operation and use of the Shandon Cryotome FE and FSE Instructions and information that applies only to a particular version of Shandon Cryotome are marked FE only or FSE only Safety Thermo products are designed for convenient and reliable operation and to accepted standards of safety The use of the Shandon Cryotome does not entail any hazard if operated in accordance with the instructions given in this manual If the instrument is used in a manner not specified by Thermo Fisher Scientific then the protection provided by the instrument may be impaired It is important for you to follow the following safety precautions A78910100 Shandon Cryotome Operator Guide 5 WIP Cry
68. otome FSE OP GUIDE A78910100 Iss4 indb 5 e 2 1 2011 10 46 53 AM Es ee Welcome THE FOLLOWING PARAGRAPHS DETAIL IMPORTANT SAFETY INFORMATION PLEASE READ THIS SECTION CAREFULLY s 1 All users must read and understand the Operator Guide and only operate the unit in accordance with the instructions If the instructions are not followed then the protection provided by the instrument may be impaired 2 Do not modify the instrument if unauthorised modifications are carried out the instrument may be made unsafe and the warranty will be invalidated 3 Potentially lethal voltages above 110V a c or 50V d c are present in the instrument 4 This instrument has a protective earth system and must be properly connected to a good earth Ground via the mains input supply 5 Donotremoveany panels or covers unless specifically instructed to do so 6 It is important that normal standards of safety and good laboratory practises are employed Always use of common sense and the best known practice when operating the instrument 7 Sharp knives and blades are used in this instrument Make sure you understand the correct methods for their installation and use 8 The centre of gravity position for the Shandon Cryotome FE or FSE is shown below 6 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 6 e 2 1 2011 10 46 54 AM Es ee Welcome
69. p section until the blade is in the required position Push the blue blade position clamp away from you to lock the top stage Anti roll plate for Note These are the recommended starting settings You may need disposable blades 5 77 minor adjustments to obtain best results S Make sure that the top edge of the anti roll plate is parallel to the blade If adjustment is necessary use a 3mm A F Allen key to loosen the two adjustment screws Move the assembly carefully until the top edge is parallel to the blade Hold the assembly firmly in position and tighten up the two screws A78910100 Shandon Cryotome Operator Guide 2 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 27 e 2 1 2011 10 46 57 AM a SSE ee Installation and Setting Up Use the height adjustment control to set the height of the anti roll plate relative to the knife edge turn it counter clockwise to raise the anti roll plate and clockwise to lower it It should be set just above the tope of the blade but small adjustments may be required when sectioning to get sections to feed under the anti roll plate Once adjusted further movements should not be required Clamp screws Adjustment screws Height adjust contrd Note Only a very small movement of the control knob is required to make a very fine adjustment If it is set too low sections will curl above the anti roll plate If it is set too high the anti roll plate will hit the block s
70. ppropriate for the knife holder s PAN SANT EN Make sure that the knife guard is correctly installed s 20 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 20 e 2 1 2011 10 46 56 AM EI CLT Installation and Setting Up IF YOU KNOW THAT A SPECIFIC VIRUS OR BACTERIA IS LIKELY TO BE PRESENT IN THE SPECIMEN MAKE SURE THAT YOU ARE AWARE OF A SUITABLE DECONTAMINANT BEFORE YOU INTRODUCE THE SPECIMEN INTO THE REFRIGERATED CHAMBER s ALWAYS MAKE SURE THAT THE WINDOW IS CLOSED BEFORE ANY SECTIONS ARE CUT s TO AVOID INJURY DO NOTMOVETHESPECIMEN HEADWHENYOURHANDS AREINTHECHAMBER S Remove the waste bottle Use approximately 200ml of a 1096 solution of formalin 496 formaldehyde solution to fill the waste e bottle sufficient to cover the outlet tube Refit the waste bottle make sure that the outlet tube fits through the hole in the lid and the end of the tube is below the level of the formalin REFER TO MATERIAL SAFETY DATA SHEET MSDS FOR FORMALIN AND FORMALDEHYDE s KEEP SUFFICIENT FORMALIN SOLUTION TO COVER THE END OF THE OUTLET TUBE IN THE WASTE BOTTLE AT ALL TIMES TO PREVENT THE RELEASE OF AEROSOLS OR CONTAMINANTS DURING USE s A78910100 Shandon Cryotome Operator Guide 21 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 21 e 2 1 2011 10 46 56 AM a SSE ee Installation and Setting Up Routine Operation Setting up the Shandon Cryotome
71. r moisture in the slot of the knife holder support plate in the base of the refrigeration chamber Air Intake The efficiency of the refrigeration system is reduced if the air intake is blocked bottom right hand side of the instrument Use a vacuum cleaner or soft brush to remove dust from the black air intake Defrosting An accumulation of frost within the cooling fins will also affect the efficiency of the refrigeration system To avoid this the instrument will automatically defrost the cooling fins every day The timing of this defrost can be programmed see Setup Screen Defrost Alternatively the defrost cycle can be started on demand Choose Defrost from the Options Screen to select either a Cryobar Defrost or a Cooling Fins Defrost The Cooling Fins Defrost cycle is used to remove the build up of small amounts of ice around the main evaporator coil and the cooling fins at the rear of the cryochamber and lasts approximately 5 minutes 70 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 70 e 2 1 2011 10 47 03 AM EN ee Cleaning and Maintenance The Cryobar Defrost cycle will defrost the cryobar only and will last for approximately 5 minutes THE CRYOBAR MAY REACH TEMPERATURES OF MORE THAN 50 C DURING THE DEFROST CYCLE 1 REMOVE THESPECIMENS FROM THECRYOBAR BEFORE DEFROSTING 2 DO NOT TOUCH THE CRYOBAR DURING DEFROSTING s To completely defrost
72. s prohibited This manual is for use only by the individuals to whom it has been made available by Thermo Fisher Scientific Contact Addresses e Anatomical Pathology Anatomical Pathology Tudor Rd Manor Park 4481 Campus Drive Runcorn Cheshire Kalamazoo WA7 1TA UK MI 49008 USA Tel 44 0 1928 534000 Tel 1 800 522 7270 Fax 44 0 1928 534049 Fax 1 269 372 2674 www thermoscientific com pathology The Shandon Cryotome range meets the following CE Mark requirements In Vitro Diagnostic Directive 98 79 EC 3 D C US Low Voltage Directive 73 23 EEC as ammended by 93 68 EEC WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 2 e 2 1 2011 10 46 53 AM Contents 1 Welcome Introduction Safety 2 Description Overview 3 Installation and Setting Up Procedure Unpacking Setting Up the Instrument Electrical requirements 4 Control Description Main control panel connections 5 Operation Description Safety Considerations Precautions 6 Cleaning and Maintenance Introduction Routine Cleaning and Maintenance Instructions 7 Trouble Shooting Introduction Instrument Function Sectioning Suggestions 8 Specification and Accessories 9 Warranty Statement Warranty Statement Declaration of Conformity Appendix A Transportation Instructions Instructions Product Safety Declaration Appendix B Approved Reagent List Appendix C Exampl Index A78910100 WIP Cryotome FSE OP GUIDE A78910100
73. sary use the rotary control to obtain fine positioning of the specimen with respect to the knife The rotary control moves the specimen 8 microns um with each notch Cutting Specimens On the Main Screen select MICRONS and use t o to set the required section thickness ALWAYS USE CUT RESISTANT GLOVES TO AVOID INJURY WHEN HANDLING DISPOSABLE BLADES S THERMO RECOMMENDS THAT THE WINDOW IS KEPT CLOSED WHEN CUTTING s To cut sections turn the handwheel smoothly The instrument performs a single cutting stroke with each full revolution of the handwheel Before you deal with any sections lock the handwheel in the 6 or 12 o clock position to move the specimen head to its lowest or highest position 64 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 64 e 2 1 2011 10 47 02 AM Es ee Operation Use the brush to discard any unwanted sections sweep them away to the sides or rear DO NOT BRUSH AGAINST THE TOP OF THE BLADE Pick up the required selections on a slide Continue to turn the handwheel to cut more sections The specimen automatically advances by the section thickness before the start of each cutting stroke and is automatically retracted by 40 microns during the return stroke Removing When the last section has been removed fit the knife guard and Specimens remove the cryocassette from the clamp using the cryocassette handle FIT THE KNIFE GUAR
74. st time and return to the Setup Screen page 44 Note During the defrost cycle the chamber temperature will rise However the cryobar will not be affected if it is enabled s A78910100 Shandon Cryotome Operator Guide 53 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 53 e 2 1 2011 10 47 01 AM a SSE ee Controls Defrost Screen Select defrost type Cooling Cryobar Fins Defrost Defrost The buttons on the Defrost Screen are described below Cooling fins defrost Cooling Fins Defrost Press COOLING FINS DEFROST to start a quick defrost which defrosts only the cooling fins When pressed the screen displays the Defrosting Screen see above This defrost lasts for approximately 5 minutes I Cryobar Defrost Cooling Fins Defrost Press CRYOBAR DEFROST to start a quick defrost which defrosts the cryobar When pressed the screen displays the Defrosting Screen This defrost lasts for approximately 5 minutes Note Remove the cryobar cover during the cryobar defrost s Press EXIT to go to the Main Screen page 36 without defrosting the instrument 54 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 54 e 2 1 2011 10 47 01 AM a SSE ee Controls Defrosting Screen Cooling Fins befrosting Cancel Defrost The Defrosting Screen shows the type of defrost cycle either cooling fins defrost or cryobar defrost There
75. switch Rating plae Note The symbol on the rating plate indicates that the instrument operates on an alternating current supply a c s Make sure that the I O power switch at the rear of the instrument is switched off O side of the switch pushed inward Instruments are supplied with power cords with moulded plugs suitable for many countries If another plug is required a technically competent person should use the wiring convention shown in the following table to replace the existing moulded plug with a suitably rated and where appropriate fused plug European cable US Cable Terminal Brown Black Live L or L2 Blue White Neutral N or L1 Green Yellow Green Earth E ground 16 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 16 e 2 1 2011 10 46 55 AM a SSE ee Installation and Setting Up THE SHANDON CRYOTOME MUST BE PROTECTIVELY EARTHED MAKE SURE THAT THE INSTRUMENT IS PLUGGED INTO A PROPERLY EARTHED GROUNDED MAINS SUPPLY THE SHANDON CRYOTOME MUST BE PROTECTIVELY EARTHED MAKE SURE THAT THE INSTRUMENT IS PLUGGED INTO A PROPERLY EARTHED GROUNDED MAINS SUPPLY s IT MUST BE POSSIBLE TO INTERRUPT THE MAINS SUPPLY AT SOURCE BY REMOVING THE PLUG FROM THE MAINS SUPPLY SOCKET s Select the appropriate mains lead from those supplied and plug it into the mains inlet socket in the rear panel of the instrument Connect the mains supply cable to the local power
76. tal specimen advance 25mm Advance Speed gt 1mm second Section thickness range 1 to 20um in increments of 1pm 20 to 60pm in increments of 5um Cutting Stroke 57mm Retraction 40pm on upward stroke Cryochamber atan ambient temperature of lt 25 C Cryochamber temperature 0 to 35 C Cryobar temperature 55 C minimum adjustable Specimen head temperature 0 to 40 C performance restricted tot 10 C from chamber temp FSE A78910100 Shandon Cryotome Operator Guide 83 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 83 e 2 1 2011 10 47 04 AM Specification and Accessories Accessories Knife blade holders Disposable blade holder with anti roll plate high profile 1 A78910033 Disposable blade holder with anti roll plate low profile 1 A78910032 Solid knife holder with anti roll plate 1 A78910047 Orientation heads X Y orientation head FE only 1 A78910016 Cryobar Accessories Cryobar cassette identification label pk of 4 A78910054 Cryobar cover 1 A78910016 Cryocassettes 40mm diameter crosshatched multicoloured pk of 6 A78910031 40mm diameter annular pk of 4 A78910055 25mm diameter annular pk of 4 77210150 25mm diameter crosshatched pk of 4 A78910056 9 5mm diameter angular pk of 4 77210153 28 X 40mm crosshatched pk of 4 A78910057 Handle pk of 4 A78910058 Debris Tray A78910039 Heat Extractors standard A78910014 half weight 1 A78910036 double weight 1 A78910037 Shelves Tool 1 A78930121 N
77. to thinner block Specimen falls off cryocassette Specimen too thick Cut specimen into thinner block Reduce time before adding mountant Cryocassette too cold before 9Shoudl be at room temperature mountant added Give the specimen more support medium e g Shandon Cryomatrix Specimen unsupported Specimen advances but does Knife loose Make sure that the knife is clamped not cut correctly Cryocassette loose Make sure that the cryocassette is fitted and clamped correctly Re mount the specimen on the Cryo Specimen not firmly stuck to the cassette using adequate mountant Cryocassette e Increase knife angle Knife angle incorrect Sections roll up Anti roll plate too low Raise the height of the anti roll plate Raise the specimen temperature Specimen too cold Warm the specimen slightly with a gloved thumb Sections thaw when cut Cutting equipment not cold enough Allow more time for the knife and the anti roll plate to cool Cont 76 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 76 e 2 1 2011 10 47 03 AM Troubleshooting Table 2 Problems when sectioning con Sections are puckered Sections appear with fine cracks parallel to the edge of the knife Sections show ice artefacts Sections show signs of the effects of vibration A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 77 The knife is dirty The knife is damaged The anti roll plate is ni
78. unger on the underside of the base section into the circular hole at the end of the support plate slot the support plate is located in the base of the chamber 24 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 24 e 2 1 2011 10 46 57 AM Installation and Setting Up Locating plunger Support plate slot Slide the blade holder assembly along the support plate to the required position and push away the black position clamp to lock it in place Position clamp BLACK Adjusting the disposable To adjust the angle of the knife holder pull the red angle clamp fully blade holder toward you Adjust the angle of the knife holder until the 0 of the angle scales is approximately in line with the marker on the base section then push the red angle clamp away from you to lock it This gives a cutting angle of 22 Angle clamp RED A78910100 Shandon Cryotome Operator Guide 25 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 25 e 2 1 2011 10 46 57 AM a SSE ee Installation and Setting Up Note The knife holder is adjusted to its precise operating angle after the blade or knife is installed s Note The blade type used may affect the optimum blade angle For Kai MX35 blades start to section at an angle of 3 on the scale and make small adjustments to achieve optimum performance s Fitting a disposable blade Be aware of the dangers of handling microtome bla
79. utton 38 set point display 39 set point values 38 specimen button 37 specimen travel indicator 39 up and down arrow buttons 38 mounting a specimen 61 moving the instrument long distances more than 2m 12 short distances 13 O on off 18 on off switch 16 Shandon Cryotome Operator Guide WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 99 e safe operating temperature 82 operation 60 cutting specimens 64 housekeeping 66 mounting a specimen 61 precautions to take 60 removing specimens 65 setting up the blade holder 64 setting up the instrument 22 options button 38 options screen 40 cryobar boost cancel boost button 42 cryobar disable enable button 43 defrost button 41 exit button 44 fumigate button 41 lock unlock button 41 setup menu button 44 unload specimen button 41 P pinchpoint symbol A 4 preparation for use 20 product safety declaration 93 protective earthing grounding 6 R re enter new code screen 52 reagent list 94 cleaning agents 94 Linistat staining reagents 94 refrigeration system 8 refrigerant 8 remote alarm 32 removing specimens 65 repacking 88 preparation 88 rotary control 35 A78910100 2 1 2011 10 47 08 AM S safety precautions 5 humidity 82 screen description 34 overall voltage category 82 security code screens 51 pollution degree 82 enter current code screen 52 recommended operating temperature 82 enter new code screen 52 safe operating temperature 82 re enter ne
80. w code screen 52 transit storage temperature 82 security modes 48 microtome 83 access code 49 advance speed 83 to select 50 cutting stroke 83 activation 48 general 83 hold key to unlock 48 retraction 83 to select 50 section thickness range 83 security off 48 total specimen advance 83 to select 50 part numbers 83 security setup screen 48 physical dimensions 81 security mode selection 48 depth 81 setup menu button 44 weight 81 setup screen 44 height 81 exit button 44 width 81 set clock screen 45 specimens 12 hour 24 hour button 46 cutting 64 set button 46 mounting 61 time and day display 45 removing 65 set point display 39 specimen button 67 set point values 38 specimen head 29 shelves 31 fine orientation head 29 siting requirements 14 fixed head 29 spares 85 specimen travel indicator 39 specification and accessories 81 accessories 84 T specification 81 temperatures centre of gravity 83 cutting termperatures suggested 95 cryochamber 83 recommended operating temp 82 cryobar temperature 83 safe operating temperature 82 cryochamber temperature 83 transit storage temperature 82 specimen head FSE temperature 83 time setting 45 electrical 81 transit bracket fuses 82 repacking 88 power demand characteristics 81 unpacking 17 supply voltages 81 transportation instructions 88 switch convention 82 product safety declaration 93 environment 82 troubleshooting 74 A78910100 Shandon Cryotome Operator Guide 100 WIP Cryotome FSE OP GUIDE A78910
81. wering knobs counter clockwise to lower the rollers to the ground A78910100 Shandon Cryotome Operator Guide 90 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 90 e 2 1 2011 10 47 04 AM e ee SHANDON CRYOTOME FE AND FSE WEIGH APPROXIMATELY 125kg 275lbs ALWAYS GET HELP TO SAFELY MOVE THE INSTRUMENT WITHOUT RISK OF INJURY s Repacking Use two people for the following steps 1 Place the packaging pallet in a convenient place so that there is enough room for the ramp to lie on the floor in front of the pallet and that there is enough room for a person to get to the back of the pallet 2 Push the instrument backwards up the ramp and make sure that the instrumnet sits inside the foam packing on the pallet The front of the instrument should face forward 3 Place the accessories box in front of the instrument underneath the footrest 91 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 91 e 2 1 2011 10 47 05 AM 4 Raise the ramp and place the foam packing piece on top of the instrument making sure that the top of the ramp fits into the slot in the foam 5 Wrap the cardboard outer around the instrument so that the join is at the front next to the raised ramp The large flap with the three holes on the edge must be under the small flap with the three plastic fixings 6 Push the plastic fixings so that they match up with the holes in the l
82. wn These buttons adjust the value of the set point associated with the highlighted selector button Micron increments 1 20um in lum steps 20 60um in 5um steps Temperature increments 1 C steps Minimum Temperature 60 C settable temperature Workable temperatures specified for an ambient temperature lt 25 C Cryochamber 0 to 35 C 38 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 38 e 2 1 2011 10 46 59 AM a SSE ee Controls Setpoint display The display located between the Up and Down arrows shows the set point associated with the highlighted selector button LJ bpm If a temperature setpoint of 0 is selected the Setpoint Display will show the word OFF as shown below and the associated compressor or specimen head will be turned off LSJ m Specimen travel indicator Specimen travel specimen head The specimen travel indicator is a graphical representation of the specimen head and the chamber as shown The picture shows the current position of the specimen head in the chamber When the specimen head is almost at the end of forward travel position the specimen travel graphic will flash Press the specimen travel indicator graphic to display the help screen Note The specimen travel indicator graphic is replaced with the ERROR button and an alarm sounded when there is an error with the instrument S A78910100 Shandon Cr
83. yotome Operator Guide 39 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 39 e 2 1 2011 10 46 59 AM a SSE ee Controls The ERROR button displays a short description of the current error It flashes to highlight the error Press ERROR to display the Error Screen that describes the current error Set Tuesday 04 12 Censor Error 1 Code 0002 Microtome fault The software has encountered a problem when_initialising e the microtome hardware Turn off and retry A service call may be required Press EXIT to clear the Error Screen and return to the Main Screen Options Screen Options menu Monday 08 02 md prse pene Setup Cryobar Cryobar Menu boost Disable Unload Exit Specimen 40 Shandon Cryotome Operator Guide A78910100 WIP Cryotome FSE OP GUIDE A78910100 Iss4 indb 40 e 2 1 2011 10 46 59 AM Es ee Controls The buttons on the Options Screen are described below Either the LOCK or the UNLOCK button is displayed in the same screen location Lock Unlock The LOCK button is only displayed when the instrument is unlocked Press LOCK to lock the window and activate the security mode selected pg The UNLOCK button is only displayed when the window is locked and the security mode is off Press UNLOCK to unlock the window ii To carry out an immediate defrost cycle press DEFROST to display the Defrost Screen from

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Cryotome FE &amp; FSE Operator Guide

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Cryotome FE & FSE Operator Guide