t50/F7000
Contents
1. AA 14 Specialty Sample Holder For a Specialty Sample Holder ID 00 controlled by a TC 1 there will be a second response to the F1 ID command F1 ID What is the ID number of the sample holder being controlled re F1 ID 00 Sample holder is a Specialty Sample Holder TERIN FL FS 4 G This response will automatically follow the F1 ID 00 response It defines the Functional Status functionality of the holder through its five parameters Each parameter defines whether the holder has or does not have the functionality as defined in the table below parameter functionality 1 Temperature Control 2 Stirring Control 3 External Probe 4 Multiple Sample Positions 5 Reference Holder As appropriate to the functionality the commands and responses in command classes 2 13 will also be available 15 Control of Automatic Reports The TC 1 can be controlled manually using the buttons and the display on the front When a computer program is in use the TC 1 will automatically send reports to the program whenever a manual change has been made The commands in this section are intended to allow the program to prevent the TC 1 from sending those automatic reports F1 XX RG 2 Stop R or start R reporting all manual changes including those starting with R1 and F2 By replacing the XX with individual command codes a program can selectively stop or s2. barb in the base of the unit It passes up through holes below the optical ports in the cuvette tower to fill the space between the cuvette and the optical mask preventing condensation on the surface of the cuvette Note A flow of dry gas is necessary any time the 50 F7000 is controlled below the dew point temperature present on the inside of the sample compartment of the spectrometer For ambient air this would typically be about 5 C 4 5 External Temperature Probe A inch phone jack labeled Probe can be found on the back panel of TC 1 Temperature Controller This jack will accept the plug on a standard Series 400 or Series 500 thermistor probe such as the YSI 423 available from Cole Parmer When a probe is plugged into the jack the probe temperature is presented on the display of the TC 1 Place the probe in the sample to measure the actual temperature of the sample which will lag in time from the temperature of the cuvette tower Excellent Series 500 probes may be obtained with diameters less than 1 mm providing access to small sample volumes A disadvantage of these probes is that they are not pre calibrated 4 6 External Computer Control All functions may be accessed either through a Serial RS 232 or a USB located on the back of TC 1 Temperature Controller You may write your own program or purchase the QNW application program T App T App will plot temperatures of the probe cuvette tower and even the Peltier element
3. heat exchanger vs 8 22 2014 Page 18 of 28 SW QUANTUM QNW 150 F7000 User s Guide W E S T time It will also permit you to set up temperature ramps If you wish to do your own programming please see the Appendix for communication instructions and the set of text commands that may be used and responses to the commands 4 7 Working at Extended Temperatures Not applicable for this product 4 8 Troubleshooting If the submersible pump has operated without being immersed in water it may not prime properly If this occurs place one drop of detergent on the pump intake and try again If the TC 1 display indicates that there is inadequate coolant see section 4 13 it may be that the circulating water is too warm Add ice to the water to cool it down If a microcuvette is used with a z height of less than 8 5 mm spectroscopic measurements may be erratic and erroneous and the cuvette may be difficult to remove Use a taller cuvette instead 4 9 Error Messages When errors occur the line 1 of the TC 1 Temperature Controller display presents an error code Line 3 of the display identifies the error and line 4 of the display presents possible solutions The most common events that cause errors to be displayed are loose cables or inadequate coolant flow For errors not easily solved contact QNW at www qnw com contact us or service qnw com Specific error messages include the following 5 cell out of range warnings loose
4. how fast the cuvette holder could reach the target temperature without ramping If you attempt to ramp too quickly especially at high and low temperature extremes you will obtain a nonlinear ramp The slowest ramp that may be set on this page is 0 01 C minute If needed much slower ramps may be set through software commands 4 3 Cooling Water for the Peltier The Peltier element or thermoelectric cooler is a heat pump When cooling it transfers heat from the cuvette tower to the heat exchanger when heating electrical polarity is reversed and it transfers heat from the heat exchanger to the tower When cooling it is particularly important to transfer this heat away from the Peltier unit This is accomplished with flowing water through the heat exchanger A source of water or other cooling fluid must be connected to the inch hose barbs on the side of the 150 F7000 The QNW BATH 10 includes a submersible pump the appropriate fittings for connecting tubing and a plastic bucket Connect the pump to the 150 F7000 place it in the bucket with water and run a return tube to the bucket A more robust pump is available as the BATH 100 although the larger pump has a higher wattage and tends to heat the water when used for extended periods of time Cooling water may also be provided from another source such as a refrigerated cooling bath or even a tap for brief use only The Peltier unit requires a flow of 100 to 300 mL min This
5. implemented through traditional RS232 serial connectors on the computer or the spectrometer and on the controller In this case they will be connected by a standard 15 pin serial extension cable male connector on one end and female on the other No driver installation should be needed Otherwise the serial linkage will be established through a USB connection between the computer and the controller In this case the controller includes electronics which convert the USB connection to a serial communications port However for the port to be available to programs on the computer it will be necessary to load driver software It is important that the driver software be loaded before connecting a USB cable between the controller and the computer Contact Quantum Northwest at support qnw com for further information Quantum Northwest can provide a control program written specifically for control of all functions of the temperature controller Ask for program T App In programming for the TC 1 controller one must adhere to the conventional notation 8 N 1 Baud 19200 Data Bits 8 Parity None Stop Bit 1 Flow Control None For many of the commands listed below the controller returns information in response to the command All commands and responses are delineated by left and right square brackets Any text sent to the controller not enclosed within brackets will be ignored In this document an ellipsis is used to distinguish respo
6. other pages will switch back to this Display page Set Temperature Target 37 0 C Current 20 0 C Figure 9 TC 1 Set Temperature page Set Temperature To set the Target temperature use the up and down arrows shown in Fig 7 Press SET to initiate temperature control 8 22 2014 Page 16 of 28 SN QUANTUM QNW 150 F7000 User s Guide When seeking a temperature the green LED on the front panel of the TC 1 will flash slowly When it has locked onto a new target temperature the green LED will remain lit A rapidly flashing red LED usually means a loose electrical cable or inadequate water flow for the Peltier unit Set Stirring Stir Speed 1200rpm Current Orpm Figure 10 TC 1 Set Stirring page Set Stirring To turn on magnetic stirring use the up and down arrows shown in Fig 7 to choose an approximate stirring speed Press SET to turn on stirring at this speed The minimum is stir speed is 400 rpm and the maximum is 2000 rpm Set Ramping Ramp 0 55 min Current 0 min Figure 11 TC 1 Set Ramping page Set Ramping To perform a ramp set the Ramping rate using the up and down arrows shown in Fig 7 and press SET With ramping set turning on temperature control by accessing the Set Temperature page Fig 9 will generate a linear ramp to the target temperature When the sample holder reaches the target the ramp rate will be set back to 0 C minute The fastest possible ramp is determined by
7. Identifier Revision Effective Date t50 F7000 MAN 0 0 8 22 14 Web Page http www qnw com t50f7000 2 Description Peltier based temperature controlled cuvette holder for use in the Hitachi F 2700 F 2710 F 4500 or F 7000 Fluorescence Spectrophotometer UANIUM N O R T H W E S T QNW t50 F7000 User s Guide Quantum Northwest Inc 22910 E Appleway Avenue Suite 4 Liberty Lake WA 99019 8606 Temperature Controlled Cuvette Holders Phone 509 624 9290 Fax 509 624 9488 Service email service qnw com www qnw com and Custom Instrumentation I QUANTUM H gt W E S 1 A QNW 150 F7000 User s Guide Notices Copyright Copyright 2014 Quantum Northwest Inc All rights reserved No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form or by any means electronic mechanical photocopying recording or otherwise without prior written permission from Quantum Northwest Inc All copyright confidential information patents design rights and all other intellectual property rights of whatsoever nature contained herein are and shall remain the sole and exclusive property of Quantum Northwest Inc The information furnished herein is believed to be accurate and reliable However no responsibility is assumed by Quantum Northwest Inc for its use or for any infringements of patents or other rights of th
8. ards may occur 2 7 Computer Requirements The T App temperature application software if purchased used for external control of Quantum Northwest temperature controllers requires a personal computer using a Microsoft Windows operating system XP Vista 7 or 8 The T App software is normally provided on a CD requiring a CD player be part of the computer However T App may also be downloaded via the Internet from a QNW ftp site Contact QNW should you wish to obtain the application by Internet 8 22 2014 Page 10 of 28 SN QUANTUM QNW 150 F7000 User s Guide H W 3 Installing Starting and Stopping the QNW t50 F7000 2 1 Installing the QNW 150 F7000 Unpacking After accepting delivery take the equipment to the installation site Quantum Northwest instruments are inherently robust and the packaging is designed to prevent internal damage However the contents form part of a precision measuring system and all packages should be handled with care In transit sharp jolts must be avoided and the packages should not be inverted or tilted unnecessarily Markings on the shipping cartons generally indicate which side of the package should be kept on top Note The 50 F7000 may have been already installed in the spectrophotometer by Hitachi High Technologies Inc Unpacking the equipment is your responsibility As the packages are opened ensure that you have received everything you ordered If there are any discrepan
9. at correctly limits the excitation and emission light The slits and blanks are discussed in Section 4 1 below Place the plastic lid on the cuvette holder OPTIONAL If you wish to monitor the temperature inside the cuvette plug a standard Series 400 or Series 500 thermistor probe not provided into the 74 inch phone jack labeled probe in the back panel of the TC 1 Place the end of the probe in a region of the sample in the cuvette where it will not occlude the spectrometer light beam Turn on the TC 1 controller using the switch on the back panel This immediately provides 12 V to the submersible pump which will begin circulating water through the 150 F7000 Check for water leaks e Figure 5 TC 1 Menu Button Use the left or right arrows on the Menu Button shown in Fig 5 to cycle through the four pages of options These options are described in detail in Section 4 3 below To turn on stirring and set a temperature go to the Set Stirrer page use the up and down arrows to set a speed or let it default to 1200 rpm and press SET to start the stirrer Then go to the Set Temperature page and use the up and down arrows to set a target temperature for example 37 0 C Press SET to initiate temperature control The temperature will then change and stabilize Stopping the QNW 150 F7000 While temperature control is running and the Start page is being displayed press SET Fig 5 to turn off temperature control To turn off stir
10. cable sensor failure The temperature controller is not receiving a reasonable response from the sensor on the cuvette tower Either the sensor has failed or a cable is not making a good connection 6 cell out of range warnings loose cable check cable The temperature controller is not receiving reasonable responses from either the cell tower or heat exchanger sensors Since it is very unlikely for both to fail probably a cable is loose 7 heat exchanger error warnings loose cable sensor failure 8 22 2014 Page 19 of 28 W Q UA MT U M QNW 150 F7000 User s Guide Zl The temperature controller is not receiving a reasonable response from the sensor on the heat exchanger Either the sensor has failed or a cable is not making a good connection 8 inadequate coolant warnings inadequate coolant water temperature The sensor on the heat exchanger is reading a temperature above 60 C Temperature control has been shut down to prevent damage to the Peltier element Either the water was too warm or the rate of flow was inadequate to draw sufficient heat from the heat exchanger 9 Invalid command The controller has been sent an invalid text command 8 22 2014 Page 20 of 28 S QUANTUM QNW 150 F7000 User s Guide H W E 5 Maintenance and Spare Parts 5 1 Maintenance The 150 F7000 and TC 1 Temperature Controller require very little routine maintenance on the part of the user For routine cleaning of expos
11. cies notify the supplier If any items are found to be damaged immediately notify the carrier and supplier You should have received e 1x t50 F7000 Cuvette Holder on sample tray sample compartment floor and front panel e 1x opaque lid e 17x optical slits blanks e 1x TC 1 Temperature Controller e 1x submersible pump e 1x bucket for submersible pump e 1x power cable e 1x USB cable e 1x CD containing T App software if purchased e 1x magnetic stir bar e 1x vinyl tubing to connect water and gas e This manual Note Any additional accessories ordered are not listed here Any differences from the original order should be referred immediately to your Quantum Northwest sales office Do not discard any packaging components or filler materials 8 22 2014 Page 11 of 28 S QUANTUM QNW 150 F7000 User s Guide W E S T Installing the QNW T App Software 1 Insert the T App program CD into the computer s CD ROM drive If the installation does not start automatically locate the Setup exe file in the root directory and run it The installation process starts with a small black window that is shown during installation of the drivers needed to control the 150 F7000 through a USB connection This window will then be replaced by the software installation window Follow the onscreen prompts to complete the installation 2 Connect the t50 F7000 to your computer using the USB cable provided The New Hardware installation process will begi
12. d e E te he E OAE 17 Dry Gas to Minimize Condensation ssis meer nnne 18 External Temperature Probe sssssssssssssssssesesseeeeneeneneme eren innere nennen nnne nets 18 External Computer Control 2 rd et p edat e ed a deter 18 Working at Extended Temperatures sssssssssssese eene nennen nemen nennen enne 19 Troubleshooting id tt great e ducto ucc eee gute pu S e tee o A agr ve b a vt eg e 19 Error Messagessi cii rH bead uite REED EEPMut 19 Maintenance and Spare Parts cccccccccseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeaaeeneeeeeeeeeeeseaes 21 Mainteriances pce e bic pl e cene e e eee pde du ebd ipd De e eee pd eue 21 Spare Parts 3 52 6 tnnt het odia eripuit mitte 21 SN QUANTU QNW 150 F7000 User s Guide BSIN O R T H W E S T Appendix Serial Communications for the TC 1 Temperature Controller 22 8 22 2014 Page 4 of 28 S Bis QUANTUM QNW 150 F7000 User s Guide W E S T 1 General Information Thank you for purchasing a Quantum Northwest 150 F7000 We want you to enjoy many years of faithful service from your instrument If you have any questions feel free to contact us directly through our website www qnw com contact us or by email at service qnw com Your Quantum Northwest cuvette holder and accessories have been carefully designed so that when used properly you have an accurate fast flexible and safe temperature control system Information about safe
13. ed surfaces use only a cloth dampened with water or diluted detergent Do not use organic or abrasive solvents CAUTION Caution Any action which makes it necessary to open the 50 F7000 or TC 1 Temperature Controller units must be executed only by QNW technicians or authorized personnel The water hoses and their attachments to the 50 F7000 TC 1 Temperature Controller and submersible pump should be inspected prior to each usage to ensure that they are intact Replace the nylon tubing when it becomes discolored or cracked Periodically replace the water stored in the bucket to minimize the growth of microorganisms When not in use the pump bucket and tubing may be stored dry 5 2 Spare Parts Vinyl tubing and magnetic stir bars may be obtained from a variety of commercial vendors For electrical cables to be used with 150 F7000 and TC 1 Temperature Controller and submersible pumps please contact QNW at service qnw com 8 22 2014 Page 21 of 28 S QUANTUM QNW 150 F7000 User s Guide Appendix Serial Communications for the TC 1 Temperature Controller This document provides the serial communications protocols for version 1 0 of the firmware on the TC 1 family of controllers used for the 50 F7000 All functions of the temperature controller can be managed from a computer using the command set described below If you purchased your unit as a component of a spectrometer from certain manufacturers this feature may be
14. ee ee Die cag 5 Modules Covers and Panels a a a aaa a ada aa aaia aaa iaa adaa daidai 5 Other Precautions nr iere aem eb e E o e E RO D p E AARE 5 Warning and Other Symbols 5 i tte ee in EU enti pt eei e o een ep eed 6 CE Compliance 2 nerd uec eee ee RR EE et px eee een 6 Specification TETTE ENTE RRTEAES 8 Introduction QNW 150 F7000 Cuvette Holder with TC 1 Temperature Controller 8 Measurement Category 1 31 iieri eed eae ou eeca b e cede ded eese e d De a e D e Aen 9 Pollution Degree exc init n ev tre Pp nte t DERE Pighius 9 Overvoltage Gategory 1 5 not futt ttn ela qi e ftu eot lr de 9 Environmental CondlitlOris coiere td d E po ter FO e ed P D edes 9 Electrical Specifications roa te bte alee a e batte de lea ve bee tae dieu 9 Computer Requirements eri iet tette ileal AE ate ada lentes cetacean EINE 10 Installing Starting and Stopping the QNW 150 F7000 e 11 Installing the QNW 150 F7000 sssssssesseseeneeneneennen nennen nnne nnne nnns 11 Starting the QNW A OF A OOO a a a r e aa tnr enne nere irren nennen 14 Stopping the QNW 150 F 7000 irni eann aa ED e E e He en HE epee ret dtd 14 Step by Step Instructions for Using the QNW 150 F7000 ssuuuuuus 15 Instr ment OVerVIGW 5e x ea RR RR UR RR A PIRE E HR ERA 15 Detailed Instr ctiloris ii gr b HE ELLE E niet qe Any rede EE 16 Cooling Water for the Peltler icto re E E P geh
15. eriodic heat exchanger temperature reports 12 Cell Changing These commands will have an effect only for a sample holder with multiple cuvette positions F2 DI Device initialize move to home position F2 PI Device initialize move to home position and reply when done AR F2 DL 1 Device is finished moving F2 DL 3 Device locate move to position 3 Device should be initialized prior to using this command for the first time F2 PI Device initialize move to home then to position 1 and reply when done I F2 DL 1 Device is in position 1 F2 PL 4 Device locate move to position 4 and reply when done Device should be initialized prior to using this command for the first time RU F2 DL 4 Device is now in position 4 F2 DD 400 Set speed to 400 acceptable range 100 900 with a default of 500 13 Reference Cuvette These commands will have an effect only for systems with two independently controlled sample holders R1 To control and monitor the temperature and status of the reference cuvette using a Dual Temperature Controller use any commands in classes 3 8 10 and 11 substituting R1 for F1 There are no corresponding R1 commands for command classes 1 2 9 and 12 If you wish to ramp the temperature of the reference and sample cuvettes together please note the linking command F1 TL in command class 10 8 22 2014 Page 26 of 28 il D QUANTUM QNW 150 F7000 User s Guide H W E S 1
16. flow should require a pressure of about 3 5 psi 0 2 0 3 bar 8 22 2014 Page 17 of 28 D QUANTUM QNW 150 F7000 User s Guide H W E CAUTION Caution Do not exceed an input water pressure of 25 psi 1 7 bar as damage may occur inside the 150 F7000 Note The heat exchanger and hose barbs are brass and the tubing inside the 150 F7000 is vinyl Be sure that any circulating fluid used other than water will not corrode these materials The temperature of the heat exchanger in the t50 F7000 is monitored using a thermistor If the temperature exceeds 60 C then temperature control is shut down to prevent damage to the Peltier element and the warning check coolant flow displayed on the TC 1 temperature controller This will happen if the circulating fluid gets too warm and or is restricted in flow The heat exchanger temperature may be accessed by computer through the RS 232 or USB connections on the back of the TC 1 Temperature Controller Temperature increases will be faster when room temperature water is used in the circulator Temperature decreases will be faster when ice water is used Only water should be circulated using the BATH 10 When using a refrigerated bath circulating pre cooled fluids such as 3096 methanol or diluted ethylene glycol at below 0 C will permit measurements below the specified temperature range 4 4 Dry Gas to Minimize Condensation Dry gas flows into the 150 F7000 via the small hose
17. gislation and am the person authorized to compile the technical documentation Signed by gend A Name Enoch W Small Ph D Position President Quantum Northwest Inc Date August 22 2014 Location Liberty Lake Washington USA 8 22 2014 Page 7 of 28 S a QUANTUM QNW 150 F7000 User s Guide Ww 2 Specifications Figure 1 t50 F7000 Cuvette Holder Figure 2 TC 1 Temperature Controller 2 1 Introduction QNW 50 F7000 Cuvette Holder with TC 1 Temperature Controller Intended Use The QNW 150 F7000 Cuvette Holder is designed to be inserted into a Hitachi F 2700 F 2710 F 4500 or F 7000 Fluorescence Spectrophotometer replacing the sample cuvette holder originally supplied with the Hitachi instrument The 150 F7000 has four optical ports for absorbance measurements and comes with the TC 1 Temperature Controller to control sample temperature during optical measurements Temperature control is achieved using the TC 1 Temperature Controller alone or along with a computer interfaced to the TC 1 Temperature Controller box using the QNW T App software if purchased Specifications e Temperature range 40 to 110 C e Temperature precision 0 01 C e Temperature accuracy 0 2 C from 0 C to 80 C e Temperature reproducibility better than 0 05 C e Cuvette size outside dimensions 12 5 x 12 5 mm e Optical port dimensions 12 mm high x 10 mm wide e Probes accepted Series 400 or Series 500 e Cuvette z he
18. ight 15 mm 8 22 2014 Page 8 of 28 SN QUANTUM QNW 150 F7000 User s Guide H W Note The z height of a cuvette is the distance between the bottom surface of the cuvette and the designed position for the optical center line where the incident beam of light strikes the cuvette 2 2 Measurement Category The measurement category is IEC 61010 l Do not to use this equipment for measurements within measurement categories II III and IV 2 3 Pollution Degree The pollution degree is IEC61010 2 Pollution degree 2 applies to a normal indoor atmosphere 2 4 Overvoltage Category The overvoltage category installation category is CAT Il See IEC 664 amp IEC 61010 2 5 Environmental Conditions The area should have a dust free atmosphere with minimal drafts vibrations and corrosive fumes For optimum performance the ambient air is recommended to be between 20 and 25 C but can be from 5 to 40 C Relative humidity should be less than 80 The instrument is designed for operation at 2 000 m elevation or less To avoid damage through spillage of solutions and samples being analyzed the worktops should be covered with a material that is corrosion resistant and impervious to liquids Allow at least two inches of space on both sides and six inches at the rear of the instruments to permit free air circulation The power cord and all other connections are located at the rear of the instruments The power Switch i
19. ird parties resulting from its use The Quantum Northwest name QNW acronym and Quantum Northwest logo are trademarks or registered trademarks of Quantum Northwest Inc All other trademarks are the property of their respective owners Quantum Northwest products must only be used in the manner described in the Quantum Northwest produce user guides Any other use may result in damage to the product or personal injury Quantum Northwest is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Quantum Northwest product user guides or use of the products in violation of applicable laws rules or regulations Quantum Northwest shall not be liable for errors or damages on products or performance due to using non Quantum Northwest genuine parts Warranty The material contained in this document is provided as is and is subject to being changed without notice in future editions Further to the Page 2 of 28 maximum extent permitted by applicable law Quantum Northwest disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a particular purpose Quantum Northwest shall not be liable for errors or for incidental or consequential damages in c
20. le corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazards which can result in death serious personal injury and damage to equipment and property ALWAYS ensure that laboratory safety practices governing the use handling and disposal of such materials are strictly observed These safety practices should include the wearing of appropriate safety clothing and safety glasses 8 22 2014 Page 5 of 28 SN QUANTUM QNW 150 F7000 User s Guide WeE SS T 1 4 Warning and Other Symbols The following is a list of symbols that appear in conjunction with warnings on the QNW cuvette holder and accessories The hazard they describe is also shown A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows When used on a warning label the user is in danger of an electrical shock When used on warning labels attached to the instrument refer to the relevant operation or service manual for the correct procedure referred to by that warning label When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives 1 5 CE Compliance Your QNW 150 F7000 cuvette holder has been designed to comply with the requirements of the Elect
21. ment must be positive without sign in tenths between 0 1 and 9 9 degrees and will work for ramps going up or down Start automatic reporting of probe temperature every temperature increment set by the command above The current probe temperature is 30 5 degrees Stop automatic reporting of probe temperature every temperature increment EMIOUANT NSEN O R T H W E S T TE F1 PT 22 3 ace F1 PT NA F1 PT F1 PA S 0 5 F1 PA ME F1 PT 30 5 F1 PA F1 PX 10 Temperature Ramping F1 RR S 2 10 F1 RR OE F1 RR 2 10 Change probe temperature returned to a precision of 0 01 degree Set the ramp rate to 2 10 C minute What is the current ramp rate Current ramp rate is 2 10 C minute For the following three commands there are no corresponding reference commands R1 see command class 13 F1 TL F1 TL F1 TL 0 Ramp the sample and reference identically Ramp the sample while the reference remains stable Ramp the sample and reference independently The default is to ramp the sample while the reference remains stable Ramping both the sample and reference at different rates or to different target temperatures see below is not possible To Ramp the temperature 1 equilibrate at the starting temperature 2 set the ramp rate 3 set a new target temperature command class 5 The target temperature may be above or below the current temperature as
22. n automatically and take a few moments to finish Installing the QNW 50 F7000 Hardware Figure 3 Submersible pump bucket electrical cable and vinyl tubing for the 150 F7000 1 Remove the existing sample tray from the Hitachi instrument Install the 0 F7000 with its attached sample tray into the sample compartment and secure it in place Note The t50 F7000 is supplied completely assembled It is necessary only to make the appropriate connections 2 Position the bucket to within 75 cm 30 in above or below the Hitachi instrument CAUTION Caution Position the bucket in a location that minimizes the risk of spilling the liquid contents 3 OPTIONAL If a gas such as nitrogen is to be used as dry gas for minimizing condensation at low temperatures secure the tank to a solid object in the vicinity of the Hitachi High Technologies Fluorescence Spectrophotometer 8 22 2014 Page 12 of 28 EN QUANTUM QNW 150 F7000 User s Guide O R CAUTION Caution When using dry gas do prevent condensation on the cuvette ensure that the working area is adequately ventilated optical port PX hose barb for dry gas front panel i electrical connector y hose barbs for water Figure 4 t50 F7000 utility connections and features 4 Cut the vinyl tubing provided into two lengths three if you will be using the dry gas purge Attach one length of tubing from the hose barb of the submersible pum
23. nses from commands Note command Purpose of the command sent to the controller TP response Meaning of the response received from the controller 1 Identify F1 ID What is the ID number of the sample holder being controlled E F1 ID 14 Sample holder is a t2 150 F7000 or other single cuvette sample holder The ID and version number are shown briefly on the display when the temperature controller is turned on Assigned ldentities ID 00 specialty sample holder 14 t2 24 2x2 34 turret6 or other multi sample holder 8 22 2014 Page 22 of 28 QUANTU HW IS N CO sis QNW 150 F7000 User s Guide S T 2 Controller Firmware Version F1 VN wait es F1 VN 1 00 3 Stirrer F1 MS TP F1 MS 2500 F1 LS F1 MS 300 F1 SS S 1000 F1 SS S 0 F1 SS F1 SS F1 SS F1 SS 1000 4 Temperature Control F1 TC F1 TC 5 Target Temperature F1 TT S 23 10 F1 TT F1 TT 71 32 F1 TT Fl TT Fl MT TE F1 MT 110 F1 LT TOUR F1 LT 40 6 Instrument Status F1 IS Iu F1 IS 0 S What is the version number of the controller firmware The controller is operating firmware version number 1 00 What is the maximum stirrer speed The maximum stirrer speed is 2500 rpm What is the lowest stirrer speed The lowest stirrer speed is 300 rpm Set stirrer speed to 1000 rpm Turn stirrer off and set the
24. onnection with the furnishing use or performance of this document or of any information contained herein Should Quantum Northwest and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the separate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Safety Notices A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met 8 22 2014 Table of Contents 1 1 1 1 2 1 3 1 4 1 5 2 1 2 2 2 3 2 4 2 5 2 6 2 7 3 1 3 2 3 3 4 1 4 2 4 3 4 4 4 5 4 6 4 T 4 8 4 9 5 1 5 2 General Information E 5 User Documentation meresan anaia arani inara e eei eee teca pie e deca pl
25. p shown in Fig 3 to either one of the water hose barbs on the 150 F7000 labeled hose barbs for water in Fig 4 Attach another piece of tubing from the second 150 F7000 water hose barb to the bucket to serve as a drain tube Add water to the bucket and lower the pump into the bucket making sure that the pump is below the water line Using the electrical cable from the pump connect the pump to the socket on the back of the TC 1 Temperature Controller The pump will start automatically when the TC 1 power is turned on later 5 OPTIONAL If you plan to work at low temperatures connect a source of dry gas typically nitrogen to release upon the cuvette windows Attach a length of tubing with 7 amp inch inside diameter to the small dry gas hose barb on the base of the 50 F7000 see Fig 4 Set the dry gas flow rate to about 50 cc min 6 Connect the 150 F7000 electrical cable to the 15 pin VGA type connector labeled Sample on the back of the TC 1 Temperature Controller 8 22 2014 Page 13 of 28 S QUANTUM QNW 150 F7000 User s Guide 7 3 2 1 3 3 W E S T Plug the power cable into the back of the TC 1 Temperature Controller and into a wall socket Starting the QNW 150 F7000 Place the magnetic stir bar in a standard 1 x 1 cm square cuvette Insert a liquid sample into the cuvette Place the cuvette in the 150 F7000 OPTIONAL Use the provided optical slits or blanks around the cuvette in a manner th
26. re provided to use as apertures for the excitation or for the emitted light 9 080 p DIRECTION Ha a 3 aniz 7 5 18 16 oni as7 a pasi 1s7 4 aise Figure 6 Optical slits and blanks quantity 17 provided with the t50 F7000 8 22 2014 Page 15 of 28 S QUANTUM QNW 150 F7000 User s Guide AA T App if purchased is a program that permits external computer control of the TC 1 Temperature Controller The temperature of the sample holder and a temperature sensed by an external probe may be plotted vs time Simple text scripts may be used to automate multiple operations T App is provided with a USB cable to connect your computer to the TC 1 Full instructions for using T App may be found in the associated Help file 4 2 Detailed Instructions Using the Menu Button control arrows 9 Figure 7 TC 1 Menu Button control arrows Use the right and left arrows of the Menu Button shown in Fig 7 to cycle through four pages Display Set Temperature Set Stirring Set Ramping Each of these pages is described below Holder 20 0 C Target 100 0 C Probe 20 0 C Ramp Off Stir On Figure 8 TC 1 Display page Display This main page shows the actual cuvette Ho der temperature the Target temperature and a Probe temperature if a probe is present The page will also show whether the magnetic stirrer or ramping function are turned on or off After a few seconds of inactivity all
27. ring go to the Set Stirrer page and press SET again After measurements are completed turn off power on the back of the TC 1 controller 8 22 2014 Page 14 of 28 Ey i QUANTUM QNW 150 F7000 User s Guide 4 Step by Step Instructions for Using the QNW 150 F7000 4 1 Instrument Overview The 50 F7000 shown in Fig 1 and Fig 5 is a Peltier effect thermostatting system for standard square cuvettes 10 mm pathlength to be used in the Hitachi F 2700 F 2710 F 4500 or F 7000 Fluorescence Spectrophotometer The Peltier effect is based on the reverse thermoelectric effect a phenomenon in which a temperature difference is caused by a flow of current through a closed circuit consisting of two different metals The cuvette requires approximately 2 mL of sample for analysis which can be stirred using a magnetic stirrer while the absorbance measurement is taking place A microcuvette having internal dimensions less than 10 x 10 mm may also be used but magnetic stirring would not be possible with this smaller cuvette The 150 F7000 is a 4 window cuvette holder designed for fluorescence measurements Rapid and precise temperature changes may be made throughout the range of 40 to 110 C Variable speed magnetic stirring is provided as well as dry gas purge on the four windows of the cuvette An opaque lid enhances control of the gaseous environment around the cuvette and reduces light leakage A variety of optical slits shown in Fig 6 a
28. romagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Quantum Northwest has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by e the CE Marking appearing on the rear of the product and e the following Declaration of Conformity The Declaration of Conformity is the legal declaration by Quantum Northwest that the product complies with the directives listed above and shows the EN standards to which the product was tested to demonstrate compliance 8 22 2014 Page 6 of 28 SN z QUANTUM QNW 150 F7000 User s Guide NI x H W E S T EC Declaration of Conformity We Quantum Northwest Inc Of 22910 E Appleway Ave Suite 4 Liberty Lake WA 99019 8606 USA Declare under our sole responsibility that the products Models TC 1 turret 6 Types Temperature Controller Temperature Controlled Cuvette Holder Are in conformity with the following standards and relevant EC directives Emissions EN 61326 1 2013 Immunity EN 61326 1 2013 EU Directive 2004 108 EC EMC hereby declare that the equipment models named above have been assessed and found to comply with the relevant sections of the above referenced specifications The units comply with the relevant requirements of the applicable Le
29. rrent temperature is 22 84 C Periodically report current temperature every 3 seconds Stop periodic current temperature reports 8 Error Reporting F1 ER F1 ER F1 ER Report the current error ER 0 No current error ER 5 Cell T out of range Loose cable Sensor failure ER 6 Cell amp heat exchanger T out of range Loose cable ER 7 Heat exchanger T out of range Loose cable Sensor failure ER 8 Inadequate coolant check flow Temperature control has shut down ER 9 bad command gt gt Syntax error on a preceding command where bad command is the command that caused the syntax error Automatically report errors when they occur Stop automatic error reports 9 Probe Status and Temperature F1 PS F1 F1 PS F1 PS F1 PT F1 PT 3 Is there an external temperature probe connected A probe is connected PR No probe is connected Enable probe status to be sent automatically when a probe is installed or removed This is the default Disable automatic sending of probe status What is the current probe temperature Periodically report the probe temperature every 3 seconds 8 22 2014 Page 24 of 28 U M QNW 150 F7000 User s Guide The current probe temperature is 22 3 degrees Probe temperature is not available Stop periodic probe temperature reports Set the increment for automatic reporting of the probe temperature to 0 5 degrees during a ramp Incre
30. s located on the front panel 2 6 Electrical Specifications Mains Supply A standard power cable is provided based on the user s country requirements 18 AWG 115 V AC US Canada 1 0 mm 220 V AC international The required supply voltage is 100 240 V AC frequency 50 or 60 Hz The mains voltage fluctuations must not exceed 10 of the nominal voltage The installation of electrical power supplies must comply with the rules and or regulations imposed by local authorities responsible for the supply of electrical energy to the workplace All power supplies for the TC 1 Temperature Controller must be single phase AC voltage three wire system active neutral earth and should be terminated at an appropriate power outlet receptacle that is within reach 8 22 2014 Page 9 of 28 S y QUANTUM QNW 150 F7000 User s Guide NN CO R W of the power cord For safety reasons a separate power outlet receptacle should be provided for each unit in the system The use of extension cords or outlet adaptors is not recommended Shock Hazard Danger of electrocution Good electrical grounding is essential to avoid potentially serious shock hazards A 3 wire outlet with ground connection must be provided for the t50 F7000 Make certain that power outlets are earth grounded at the grounding pin CAUTION Caution Never connect or disconnect any cables while the t50 F7000 TC 1 Temperature Controller is switched on Damage to the printed circuit bo
31. soon as it is set the ramp will begin up or down to that new target After reaching the target the controller will hold at that temperature At any time a new ramp rate and target temperature can be set to start a new ramp Once you are done set the ramp rate to 0 Otherwise setting a new target temperature later will initiate a ramp to that target temperature Notes The minimum settable ramp rate is 0 01 C minute 8 22 2014 Page 25 of 28 SN n QUANTUM QNW 150 F7000 User s Guide IN O Re Wwe Zil For higher ramp settings the observed rate may be lower than that calculated from RT and RS or it may be nonlinear over part of the temperature range because the maximum possible rate of heating or cooling is limited and dependent on the ramp direction as well as on the temperature When the ramping process is completed the controller will send a response of the form F1 TT as a notification of the end of the ramp This response can be blocked at any time by previously sending the command F1 TT See command class 5 11 Heat Exchanger Temperature F1 HL What is the high temperature limit for the heat exchanger oraus F1 HT 60 The heat exchanger high temperature limit is 60 C F1 HT What is the current temperature of the heat exchanger eee as F1 HT 39 The current heat exchanger temperature is 39 C F1 HT 3 Start periodic heat exchanger temperature reports every 3 seconds F1 HT Stop p
32. speed to zero Turn stirrer on and set it to the most recent non zero stirrer speed setting Turn stirrer off What is the current stirrer speed setting Stirrer speed setting is 1000 rpm Turn temperature control on Turn temperature control off Set target temperature to 23 10 C What is the current target temperature Target temperature is 71 32 C Turn on automatic reporting of manual changes to the target temperature Turn off automatic reporting of manual changes to the target temperature What is the maximum target temperature allowed The maximum target temperature allowed is 110 C What is the lowest target temperature allowed The lowest target temperature allowed is 40 C What is the current instrument status Response is four parameters number of unreported errors is 0 0 to 9 8 22 2014 Page 23 of 28 S li 9 Zil F1 IS F1 IS F1 U O R UM A N T QNW 150 F7000 User s Guide we stirrer is off is on is off temperature control is on is on is off temperature is stable S is stable C is changing Automatically report instrument status whenever it changes e g due to manual changes at the controller Stop automatic reports of instrument status IS R The controller has been powered off and back on again 7 Current Sample Temperature F1 CT F1 CT 3 F1 CT F1 CT 22 84 What is the current temperature of the holder The cu
33. tart reporting of manual changes F1 SS R Stop reporting manual changes for the sample stirrer F1 TC R Stop reporting manual changes for the sample temperature control F1 TT R Stop reporting manual changes for the sample target temperature F1 PR R Stop reporting manual changes for the external probe connection F1 RR R Stop reporting manual changes for the sample ramp rate F2 PL R Stop reporting manual changes for the sample position R1 SS R Stop reporting manual changes for the reference stirrer 8 22 2014 Page 27 of 28 SW QUANTUM QNW 150 F7000 User s Guide W E S T R1 TC R Stop reporting manual changes for the reference temperature control R1 TT R Stop reporting manual changes for the reference target temperature R1 RR R Stop reporting manual changes for the reference ramp rate 8 22 2014 Page 28 of 28
34. ty practices appears throughout the documentation provided with your instrument and accessories to help you safely operate the instrument and accessories Before using the instrument or accessories you must thoroughly read these safety practices ALWAYS operate the instrument and accessories in accordance with these safety practices 1 1 User Documentation You have been provided with the following documentation to help you set up and operate your Quantum Northwest t50 F7000 cuvette holder e This manual with safety practices and hazards information instructions for installing and maintaining the components of the QNW 150 F7000 and troubleshooting information e The T App if purchased Help file located on the T App CD Quantum Northwest T App Help 1 2 Modules Covers and Panels The spectrophotometer module that operators and other personnel will routinely access is the instrument s sample compartment which is designed to contain the QNW 150 F7000 Customers may insert cuvettes and cuvette accessories into the QNW 150 F7000 while the sample compartment is open Any other panels or covers that are retained by screws on the cuvette holder and accessories may be opened ONLY by Quantum Northwest service technicians 1 3 Other Precautions Do not block any ventilation grills present on the QNW TC 1 Temperature Controller Use of the QNW 150 F7000 system and accessories may involve materials solvents and solutions that are flammab
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