cryostat Cm1900_en
Contents
1. Kryostat Instruction Manual Leica CM1900 V5 1 English 11 2001 Always keep this manual near the instrument Read carefully prior to operating the instrument Seica MICROSYSTEMS 1 Introduction etca MICROSYSTEMS Leica Microsystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Tel 06224 1430 Fax 0 6224 143 200 eMail histo_info leica microsystems com Internet http www histo solutions com Leica CM1900 Cryostat The information numerical data notes and value judg ments contained in this manual represent the current state of scientific knowledge and state of the art tech nology as we understand it following thorough investi gation in this field We are under no obligation to up date the present manual periodically and on an ongo ing basis according to the latest technical develop ments nor to provide our customers with additional copies updates etc of this manual For erroneous statements drawings technical illus trations etc contained in this manual we exclude li ability as far as permissible according to the national legal system applicable in each individual case In par ticular no liability whatsoever is accepted for any fi nancial loss or consequential damage caused by or related to compliance with statements or other infor mation in this manual Statements drawings illustrations and other informa tion as regards contents or technical details of the2. 112A T1 2 lI 1800 J s UL CSA 208 V AC 60 Hz 1800 VA 30 A eff T12A T12A T1 2 lI 1800 J s Refrigeration Cryochamber Temperature range Refrigeration capacity Cut off pressure Safety factor Refrigerant Compressor oil Defrosting of cryochamber Automatic defrosting Programmable Defrosting intervals Defrosting period Automatic stop of manual defrosting Manual defrosting Defrosting period Automatic stop of manual defrosting Quick freeze shelf Max temperature Number of quick freeze stations Specimen cooling Temperature range Refrigeration capacity Cut off pressure Safety factor Refrigerant Compressor oil Defrosting of specimen head Automatic defrosting Manual defrosting Defrosting period Automatic stop of manual defrosting acc to CECOMAF liquid temperature 45 C evaporation temperature 25 C CM1900 50 Hz 0 35 C 3 K at an ambient temperature of 22 C 690 W 25 bar 3 275 g 5 g refrigerant R 404A 0 6 EMKARATE RL22S ICI yes 1 defrost cycle 24 hours 9 minutes at 5 C 9 minutes at 5 C 43 C 0K 2K 10 10 50 C 2K at an ambient temperature of 22 C 320 W 25 bar 3 210 g 5 g refrigerant R404A 0 41 alpha 22 Kyodo 10 minutes 10 min after reaching 20 C 230 V AC 60 Hz 1800 VA 25 A eff T12A T10A T1 2 lI 1800 J s 240 V AC 50 Hz 1800 VA 25 A eff T8A T10A T1
3. 14 Troubleshooting Problem Cryostat inoperational Specimen disc cannot be removed No or insufficient refrigeration of the cryochamber Sliding window collects condensation No or insufficient refrigeration of the specimen cooling system Lamp does not work a4 Causes Mains plug not properly connected Defective fuses or automatic fuse has triggered Moisture on the underside cause the specimen to freeze to the freezing shelf or specimen head Stopper not placed in drain hole Cooling system or electronic drive defective Air humidity and room temperature too high Cooling system or electronic drive defective Lamp defective Switch defective Remedies Check if properly connected Replace fuses or switch automatic fuse back on If not possible call technical service Apply concentrated alcohol to the contact point Replace the stopper Call technical service Comply with the requirements for the installation site Call technical service Check lamp and replace tt if necessary Call technical service Instruction manual V5 1 11 2001 15 Temperature Selection Chart in minus C Tissue 10 15 15 25 25 50 Adrenals Bone marrow Brain Bladder Breast fatty Breast little fat Cartilage Cervix Fat Heart and vessel Intestine Kidney Laryngeal ib ive ee ee ee ng ee ee ee lymphoid o Musclular O R Nose ee ee E Pancreas a a Prostat
4. 2 lI 1800 J s 100 V AC 60 Hz 1800 VA 30 A eff T8A T15A M3 2 lI 1800 J s 100 V AC 50 Hz 1800 VA 35 A eff T15A M3 2 lI 1800 J s CM1900 60 Hz 0 35 C 3 K at an ambient temperature of 22 C 690 W 25 bar 3 265 g 5 g refrigerant R 404A 0 6 EMKARATE RL22S ICI yes 1 defrost cycle 24 hours 9 minutes at 5 C 9 minutes at 5 C 43 C 0K 2K 10 10 50 C 2K at an ambient temperature of 22 C 320 W 25 bar 3 210 g 5 g refrigerant R404A 0 41 alpha 22 Kyodo 10 minutes 10 min after reaching 20 C according to IEC 1010 UL 3101 Instruction manual V5 1 11 2001 Microtome Type Section thickness setting Specimen feed Knife holder repositioning on the microtome base plate Vertical stroke Specimen retraction Maximum specimen size Cryostat Dimensions Width w o handwheel Width incl handwheel Depth cabinet only Depth cabinet incl mains plug Overall height Working height Weight instrument only Weight incl accessories Set up conditions Distance to walls and furniture calculated from the cabinet Plug the instrument only to power sockets with ground Mains cable length Extendable 4 Technical data Microtome encapsulated in the cryochamber 0 60 um Infinitely variable 25mm 30 mm 59 mm no 40 mm 790 mm 890 mm 725mm 800 mm 1200 mm 1000 mm 168 kg 170 kg rear 10 c
5. 42 Causes Cryostat is exposed to air currents open windows and doors air conditioning Frost built up by breathing into the cryochamber Specimen not cold enough Anti roll plate not yet cold enough thus warming the section Specimen too cold Static electricity air currents Specimen not cold enough Large area specimen Anti roll plate poorly positioned Anti roll plate poorly aligned with knife edge Incorrect clearance angle Blunt knife Dirt on knife and or anti roll plate Top edge of anti roll plate damaged Blunt knife Anti roll plate does not protrude far enough beyond the knife edge Anti roll plate protrudes too far beyond the knife edge and Is scraping against the specimen Knife damaged Edge of anti roll plate damaged Specimen insufficiently frozen onto the specimen disc Specimen disc not clamped tightly Knife not clamped tightly enough Specimen has been sectioned too thickly and has detached from disc Very hard inhomogeneous specimen Blunt knife Knife profile inappropriate for specimen cut Incorrect clearance angle Brush forceps and or cloth too warm Remedies Change place of installation for the cryostat Wear mouth protection Select lower temperature Wait until knife and or anti roll plate have reached chamber temperature Select higher temperature Remove cause Select lower temperature Trim the specimen parallel increase section thicknes
6. Decontamina tion must be explicitly be confirmed by the customer Our service engineers have to enquire about this b Ensure that there is no radioactivity or hazard ous bacteria present and advise Leica of any de contamination procedure that may have been car ried out e Should the instrument or any part of it be received in a condition that Leica considers to be a poten tial biological hazard the instrument or part will be returned unrepaired at the expense of the cus tomer e When requesting a service call please provide the following information a Model type and serial number of the instrument b Location of the instrument and the person to contact c The reason for the service call 49 Notice 50 Instruction manual V5 1 11 2001
7. lamp e Slightly lift the sliding window 1 holding it by the grip 2 and pull it out to the front If the lamp is broken it must be replaced by the technical service as the replacement in volves a high risk of injury ig ra a j bon Removal of the lamp e The lamp is mounted behind a glare shield and ea therefore not visible i e Touch the lamp for better orientation e Lightly tilt the flourescent tube down to the left and pull it out of the clip 3 e Hold the lamp with both hands and pull it to the left out of the holder 4 i li A Only use lamps of the same specification i Installation of the new lamp Type 230 V 50 Hz OSRAM DULUX S 11 W 120 V 60 Hz OSRAM DULUX S 13 W Hold the lamp in the correct mounting position as shown and push itto the right until it engages in the holder e Replace the sliding window e Reconnect the instrument to the mains power and turn it on Leica CM1900 Cryostat 41 14 Troubleshooting Problem Frost on chamber walls and microtome Sections smear Sections splinter Sections not properly flattened Sections not properly flattened despite correct temperature and correctly aligned anti roll plate Sections curl on anti roll plate Scraping noise during sectoning and specimen return movement Ridged sections Chatter during sectioning Condensation on anti roll plate and knife during cleaning
8. present manual are notto be considered as warranted characteristics of our products hese are determined only by the contract provisions agreed between our selves and our customers Leica reserves the right to change technical specifi cations as well as manufacturing processes without prior notice Only in this way is It possible to continu ously improve the technology and manufacturing tech niques used in our products This document is protected under copyright laws Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods including any electronic systems and media requires express prior permis sion in writing by Leica Microsystems Nussloch GmbH For the instrument serial number and year of manu facture please refer to the name plate at the back of the instrument Leica Microsystems Nussloch GmbH 2 Table of contents aP SNS 10 11 12 13 14 15 16 17 18 Modic CUD oes choca scene sees E O T T E E 3 TapE or COMETE aon E uccie tes pcn es anacc nnce nc s eancaan ta ca ee neta canes ened ev dant cnncendesteates 4 Safety instructions for handling the instrument s ssssusususnunununnnnnnununnnnunununnnnnnununnununununnnnununnnnnnnnnunnununnanunnnnunnnnunnanannnnnn 5 WEG UU eR da ios 8 General tals t he ieee eee nee ec
9. Cs the Nand TS Cas secinrinnsstscacioconaseescuuceovonssstaciiuatudetucissattiousastcubseradnanexecin iota debichinisarunicdotst eicsdenisntasasnasuvaeseaienstiete 17 ips Mounting the heat extra fOr seve deuetanssscocorvaucusecateustysee snsianortuncesssiou a EEEa A EA EaU 17 7 6 PSE ALIVE the AC CES SOMES 5s sass cetich ces donpsenestansenastetutnesiiseasencdionses dcaadottan a ai a Caoa aii 18 1 1 Inserting the optional CS SO VC Sasa ccetesarngase penton can sna estteasasteac ape initestveiamn taaueaieastaiaitecenoansinteteoneciorues merit deoteanteseoiornas 19 Opera ON Heeereeetene reper tere freer eerettme creer aery cere cern te er eee tees eee rere er coer tr ere re etree tree ere rere 20 8 1 Frecoolina TMe KNTE sceoin soins oni nnn ane E EE NEE E TE EE EO EE NNE 20 8 2 Installing the knife holder base wie cccscescsscsscsscsscessussecessessesseceuseseuseucesseseuseuseucevsesensauedsessesenseuseuseuceusauensensensansansanses 20 8 3 PINS CG the kne holde Pecca E S 21 8 4 Connection to THE MAINS woicceccccccccsccscescescsscsscsseeseecescevsuseeseuceuseveuseusecceuseveuseuceucedeeseuseuseueeseesensenseuseusenseseseesensensensansensensnses 21 8 5 Turning on the instrument eieesscoistactisvcusnsnsorensicavapielacesassestnte eaters thisthateeadided viaincsbesiwucaitinionn aa bussseave taba aindelaantauauseneaiaaiwtans 21 86 Leica CM1900 Oy NU inet ins snus ca aeetat aceite tnt cheers cel cons aE 22 8 7 Programming the desired values asic putsecesavrnc enna adet
10. g Specimen disc 50 x 90 mm other sizes on request Accessories OCT cryocompound bottle of 125 ml Thermal block Leica Cryofect disinfectant spray Glass anti roll guide for knife holder CE Replacement plate for anti roll guide 50 mm for knife holders CN CS Replacement plate for anti roll guide 55 mm for knife holders CN CS Replacement plate for anti roll guide 70 mm for knife holders CN CS Anti roll guide complete assembly for knife holders CN CS Cryostat oil No 407 50 ml Adapter for Miles TissueTek specimen discs Orienting specimen head Storage set including waste container complete consisting of storage shelf left storage shelf right waste container section waste tray 48 0419 18649 0419 30383 0419 30390 0502 29553 0502 29551 0419 26140 0419 19787 0419 20971 0419 19455 0419 25681 0419 25171 0370 08636 0416 19275 0370 08587 0370 08673 0419 26491 0419 26750 0201 08926 0398 18542 0387 30795 0358 13583 0419 19506 0419 26489 0419 26937 0419 26938 0336 06098 0436 26747 0452 28800 0452 28763 0452 27904 0452 27905 0452 27906 0452 27907 Instruction manual V5 1 11 2001 18 Appendix Product changes e Due to a policy of continuous improvement of our products Leica Instruments reserves the right to change specifications without notice Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive
11. parts and accessories not supplied by Leica can under no circumstances be considered as inspected and or approved by Leica Installation or use of any such parts may impair the tech nical design features and thus the properties of the in strument Warranty claims can only be made as long as the prod uct or system has been operated according to its desig nated use and according to the instructions given in this manual In case of justified claims the manufacturer is entitled to repair or replace the goods Rescission of the sales con tract can only be demanded after two unsuccessful at tempts at repairing the instrument on the part of the manufacturer Leica CM1900 Cryostat Disposal e The instrument or parts of the instrument must be disposed of in compliance with local laws Technical service information e f you require technical service or replacement parts under warranty please contact your Leica Sales Representative or Dealer from whom the in strument was purchased e Besure to state the model type serial number and date of delivery Leica Instruments GmbH Germany cannot accept goods returned without official au thorization e fan instrument or any part of it is to be returned to Leica please note the following a If the instrument or any part of it has been ex posed to or been in contact with potentially patho genic or radioactive materials itis essential to de contaminate the instrument or part
12. 46 16 1 Orienting specimen head saci sesccsssesed erset cacetcches eceeedtaiattostows ican eds aiee dtaclostacaledeascatsiatuintt nce aod dbwedeatecads tend odneetateanidtenles 46 Ee WE TAMN GUID OOM o E E E E A E E 4 Ordering information ee 48 ADDEN can A te actetcestees tenors 49 Instruction manual V5 1 11 2001 3 Safety instructions for handling the instrument Unpacking and installation i To ensure an adequate cooling capacity the instrument must be set up maintaining a minimum distance from walls and furniture see Technical data The instrument must be transported in an upright position or slightly tilted max 30 To ensure a safe transportation with a fork lift 3 people are required one operating the fork lift and the other 2 holding the instrument on either side to prevent it from sliding down Before connecting to the mains power please check if the local voltage complies with the power rating specified on the name plate of the instrument see also Technical data Plug the instrument only to power sockets with ground After transporting wait at least 4 hours before turning the instrument on This waiting period is necesssary to allow the compressor oil which may have been displaced during transport to return into Its original position Failure to comply with this will cause severe damage to the instruments Due to the weight of the instrument 170 kg 4 people are require
13. ake outthe semicircular slit cover 7 Pullthe microtome forward until the cable becomes reachable Disconnect the cable and pull the microtome out of the guidance and remove it fromthe cryochamber Keep in mind that the microtome Is heavy Leica CM1900 Cryostat 37 12 Reinstallation of the microtome 12 1 How to return the microtome to the cryochamber A The microtome must be entirely dry before re installation Humidity inside will condense and freeze causing malfunctions and dam age Dry all parts completely before reinserting them in the cryochamber The cooling chamber must be entirely dry when turning on the instrument Frost forma tion When removing the microtome for cleaning or disinfection please keep in mind the safety instructions in chapter 10 Cleaning and Dis infection 1 Insert the microtome in the cryochamber Connect the cable before pushing the microtome completely back 38 2 While pressing the microtome against the left guid ance tighten the central screw with the Allen key 3 Mount the semicircular slit cover from behind on the front panel of the microtome Instruction manual V5 1 11 2001 12 Reinstallation of the microtome 4 Mount both parts together on the specimen cylin 6 Tighten the specimen head with the Allen key no 5 der of the microtome by turning it clockwise 5 Mount the specimen head on the specimen cy
14. cir cuit e Connect the mains plug to the mains power outlet at the wall After transporting wait at least 4 hours be fore turning the instrument on This waiting period is necessary to allow the compressor oil which may have been dis placed during transport to return to its origi nal position Failure to comply with this can cause severe damage to the instrument 8 5 Turning on the instrument e Bring the switch of the automatic fuse 2 to the upper position e Turn onthe mains switch 1 e Close the sliding window To avoid frost formation always put the cover on the quick freeze shelf Always cover the quick freeze shelf during breaks and overnight 21 8 Operation 8 6 Leica CM1900 Overview Display of Actual Temperature and Set Temperature of the specimen head display flashes during specimen head defrost display alternately reads LL Actual Temperature while Max Cool is activated flasing decimal points Indicate that specimen head refrigeration is disconnected Display of Actual Temperature and Set Temperature of the cryochamber display flashes during chamber defrost flasing decimal points indicate that chamber refrigeration is disconnected Display of Real Time Defrost Time and Error Messages When display reads defrost time the two LEDs flash The LEDs are extinguished when the control panel is locked via the Key button LEICA GiM1900 ON OFF butt
15. d for transportation 2 people per handle Leica CM1900 Cryostat Microtome knives Take care when handling microtome knives and disposable blades The cutting edge Is extremely sharp and can cause severe injury Never leave knives and knife holders with a knife blade mounted lying around Do not place a knife on a table with the cutting edge facing upward Never try to catch a falling knife Prior to manipulating the knife and specimen or changing the specimen or knife always lock the handwheel and cover the cutting edge with the knife guard Always lock the handwheel and cover the cutting edge of the knife with the knife guard during breaks Avoid contact with cold parts of the instrument as this can cause frostbite 3 Safety instructions for handling the instrument Cleaning and disinfection Turning the instrument back on Removal Reinstallation of the microtome It is not necessary to remove the microtome for disinfecting the cryochamber however removal is possible if required When disinfecting please take appropriate protec tive measures gloves mask protective clothing etc When using detergents and disinfectants please comply with the safety precautions of the disinfec tant manufacturer Only use acetone for cleaning the plastic anti roll plate of the knife holders CN and CS The glass anti roll plate of the knife holder CE can be cleaned ei ther with acetone or alc
16. e a a kooo Ovary i a Recta ee ee ee Skin with fat a Skin without fat a ae eee Spleen or bloody tissue Testicular Thyroid ee ee k Tongue a ee Uterus curettings o RO The temperature values given above are based on long term experience however these are only ap proximate values as any tissue may require particular adjustments Leica CM1900 Cryostat 45 16 Optional accessories 16 1 Orienting specimen head Mounting the orienting specimen head 1 46 Loosen the specimen head 1 turning the Allen key no 5 counterclockwise Pull out the specimen head 1 carefully and place it on the base Loosen 4 screws on the insulating ring 2 with an Allen key no 3 Remove insulating ring and adapter 3 Mount insulating ring 2 on the short adapter 3a of the specimen orientation and fix both parts with the 4 short screws to the corresponding threaded bores on the cylinder Mount the specimen head 1 on the insulating ring 2 and fix it with the Allen key no 5 Insertthe specimen orientation 4 in the specimen head 1 and fix the clamping screw 5 Insert the specimen disc 6 and fix the clamping screw 7 The specimen can be oriented when the clamping lever 8 is loose Tighten lever 8 after orienting the specimen Instruction manual V5 1 11 2001 16 Optional accessories 16 2 Thermal block e The thermal block 1 facilitates the removal of the fro
17. e access to the handwheel e Room temperature always approx 22 C e Air humidity must not exceed 60 e Distance to walls and furniture calculated from the cabinet rear 10 cm right side 20cm leftside 10cm e No heat dissipating appliances around High room temperatures and excessive air humidity affect the cooling capacity of the cry ostat and lead to the formation of condensa tion water in the instrument 14 Instruction manual V5 1 11 2001 7 Installation 7 2 Transport to the desired site Relocation e First check if the location meets the conditions specified in Syte requirements e Transport the instrument to the desired location e Observe the following Leica CM1900 Cryostat Transport on wheels e Qn even floors and for short distances the instru ment can be transported on wheels Before trans portation screw the adjustable feet 1 completely down with the open end wrench no 16 e The instrument can be rolled lengthwise and slightly be tilted forward see arrows Although the in strument cannot be rolled sideways It can be pushed carefully in that direction When tilting the instrument 2 people must counterbalance from the frontside to prevent the instrument from falling down and causing severe injury BO i i ae ae EL N Lengthwise movement 3 e When transporting the instrument on wheels 2 3 grip the cabinet only at the marked locatio
18. e other freezing stations lift the ring 1 remove the heat extractor from the fixing device and put It on the desired station After freezing the specimen insert the heat extrac tor back in the fixing device and place It in Its origi nal position on the low temperature stabilizer 2 The low temperature stabilizer cools down the heat extractor to the lowest temperature possible thus ensuring maximum performance when freezing specimen Instruction manual V5 1 11 2001 9 Daily operation 9 3 Activating deactivating the specimen 9 5 Inserting the specimen discs in the cooling specimen head e Thespecimen cooling is activated by subsequently pushing the or button in the control panel for Prior to manipulating the knife and specimen Specimen cooling and the key button or changing the specimen or knife always e Deactivation of the specimen cooling is done inthe lock the handwheel and cover the cutting same way edge with the knife guard e First lock the handwheel To activate deactivate the specimen or cryochamber cooling you must push the rel Bring the handle to the upper position The locking evant buttons subsecuently Do not push sl pin must meet the black mark on the cabinet multaneously e Push the locking pin into the recess at the handwheel 9 4 Activating deactivating the e To control if the locking mechanism has engaged cryochamber cooling try to move the handwheel slightly back and fo
19. ee ee ee ee ee eee 10 5 1 Designed use improper handling ccccecssssesssssssscsssesesssssssssssesssscsssesaesesessssesesassesevacsesesassesassesassesassesasaecesansesasensasansesas 10 o2 dar EVE y earran E ee ee ee 10 5 3 Optional models ecaccsscsanxasctnanngeisernauananesainavesatpavassiasiasuiniasntsapasadnarasnananesninanasstnanantiasnesaileneeniagossarantiaatenrmastansentaasiesmpninstanasnitas 10 a OPO aCe S ONE E O 10 Unpacking the instrument issin aE E A 11 6 1 Howto open the ciate ceiaio ie vont ed vest va snevncelunstdelevaeie Sua eentan icin deh daca eG pandas saurtdies Suaauiesapvotsaveseuddintntdghanenddeuduoannahatnadibte 11 6 2 How to remove the packing materia c cccccccccccsccsssscscsssssssscssceseeseusescesseseesessessuseucesseseuseuseusessuseeseuseuseuceusauensensensansansenees 11 6 3 Mounting TIVE ram D serais E E E 12 6 4 Transport to the installation site ss aciedccacsterdininaiverisustsaispeitelnmsinsievetsnstseshbaweessnuinansteaessatbenesatbessatuaianadsuecdtnnndteamdendenties vent 13 meallan siar 14 7 1 BF We CH UNI 0 Sacco cats a eee cee E ge carte dame tga ee cacies E E ace esate E 14 71 2 Transport to the desired site Relocation wo ccc cc cccccscssssscsscsserssesessessucsucesseseusesseseeseusessessesseseesensenseueesesensensneaseens 15 71 3 Assembly OF the handwheel cs ciscrsnnscinsdtusarsereinosansinancneseonsutbtnanstnontautitevedunutinisiuyntbdusle tial nora oewueiataaduedtavenientunteanientuanivens 17 74 LOG KM
20. g turns back on 2 8 Operation Before defrosting the specimen head remove all samples Manual defrosting of the specimen cooling A g e Push the manual defrost button with the melting snowflake on the left over the key button to acti vate the defrost cycle of the specimen head Activation is confirmed by an audible signal Then push or button on the panel for the speci men temperature There is a flashing indication of the specimen tem perature during the whole defrost cycle For 10 minutes the specimen head is adjusted to a temperature of 20 C and 30 C Subsequently the instrument adjusts to the speci men temperature which has been programmed prior to the manual defrost cycle lf necessary push the manual defrost button again to deactivate the manual defrost cycle Display lock 28 Push the key button for 5 seconds to protect the programmed values against unintended alterations The programmed values cannot be modified after having pushed the key button Push the key button once more for 5 seconds to unlock the display When the display is locked the LEDs between the hour and minute indication on the time panel are turned off Key button Is also used for turning on off the cham ber and specimen cooling For this purpose push the or button of the cor responding panel and subsequently press the key button Instruction manual V5 1 11 2001 8 Operation Erro
21. iniassisiinenntaamaaddeneanaieys 35 IOI Geanno cd at I 81 Fo OIL o 61 HON enaere E ee T 35 10 2 Turning the instrument back ON ccceccccccscsssscsecsssessecsececseseuceucevseveesecseseuseusessevenseeseusevensceuseseesensensensausassasensensansansanees Do Removal of the microtome cccccscsscsscscsesssssssecsessecsessersesseseseescesseeseeseesaesnsauecesseeseeseaesersueseesaeesessnsseesessanscnseeseaesans 36 11 1 HOW to remove the MICFOTOME cccccesssescsscscsessesscsecessecessncecsecessesesseveesesensessnsessnsassrseeseseesesersassasensesensaecesensessnsassateneess 36 Reinstallation of the microtome cccccssssssssssessessssseecesseeseeseessesescesoussesserseeseeseesesaesusenssaessaeseesaeesaesansceesessnscnssessansans 38 12 1 Howto return the microtome to the cryochamber c cccccsccccscsessscscsssscssssssecseeeceseusessessuceueeseeseuseuseuseusessessnsensansanees 38 E ING aE E geese suet A EE E E E A E A diese ET EA A OE TA tients 40 131 MONA eet IVE UBC NGI soani Ao S aeia 40 12 2 R placementofthe La MP sasesczers scatessossssnvesincastneuasncuniesancanaainvalesnananwicanitautieaasiaetaainssubeiaasitadsieaujttaita se teeta E Ea 41 ICED VS UG OUI oa ast esaecaacavscetvsntocs sans san vatessieersenvestiensentepinaeteeteteees 42 Temperature Selection Chart in minus C ou se eeee ee eeseeseeeeeeeeeeenaseseeseeeeesaeanaeaseses seseeasaeseeesanassenseananeass 45 Optional accessories misanan eane ee ee eee ere ee eee eee re ere
22. inside the cryochamber 20 e Slide the guide 2 of the knife holder base 3 onto the T piece 4 of the microtome base plate as shown The knives are extremely sharp Handle with care Never try to catch a falling knife e To clamp relocate the lever 1 backwards e fthe lever cannot be relocated entirely for clamp ing lift it up and shift it to the next position Instruction manual V5 1 11 2001 8 Operation 8 3 Inserting the knife holder e To insert the knife holder unlock the lever 1 turn ing it upward slightly to the rear e Insertthe knife holder 2 onto the knife holder base 3 as shown e To clamp turn the lever 1 forward 8 4 Connection to the mains AN During the start up of the compressor the nominal voltage must not drop below the val ues specified in the Technical Data Please note that the compressor requires a start up current between 30 and 40 A Therefore the electric circuit at the installa tion site must be inspected by an electrical engineer to ensure that it meets the require ments for a troublefree operation of the instru ment Failure to comply with the above will cause severe damage to the instrument e Check mains voltage and mains frequency to com ply with the specification on the type plate AN Plug the instrument only to power sockets with ground Leica CM1900 Cryostat e Donotconnectother consumers to this electric
23. lin 7 Insert the section waste tray der Leica CM1900 Cryostat 39 13 Maintenance 13 1 General maintenance 40 The microtome is virtually maintenance free To ensure a smooth operation of the instrument over several years we recommend the following Have the instrument inspected by a qualified ser vice engineer authorized by us once a year Enter into a service contract at the end of the war ranty period For further information please con tact your local Leica service center Clean the instrument dally From time to time lubricate the seat of the heat extractor 1 the clamping piece T piece 2 and the clamping lever 3 on the microtome base plate as well as the speci men cylinder 4 slightly with cryostat oil Press the coarse feed button 5 to move the specimen cylin der forward apply some drops of oil on the cylin der and move it back to the final home position Clean the ventilation slits of the liquefiers on both sides of the instrument with a brush broom or vacuum cleaner to free from dust and dirt in the di rection of the fins Do not carry out any repairs on your own as this will invalidate the warranty Repairs may only be carried out by qualified ser vice engineers authorized by Leica Instruction manual V5 1 11 2001 13 Maintenance 13 2 Replacement of the lam p p Turn the instrument off with the mains switch and pull the mains plug before replacing the
24. m rightside 20cm left side 10 cm 3 5m no The installation site must be free of draft and insolation the floor must be free from vibrations Lamp 50 Hz Version 60 Hz Version Working temperature range 0 C to 35 C 3 K Minimum ambient temperature 18 C Maximum ambient temperature 40 C Maximum relative humidity of air 60 Leica CM1900 Cryostat Osram DULUX S 11 W 21 Osram DULUX S 13 W 21 Refrigerant and compressor oil must only be replaced by authorized service personnel 5 General description 5 2 Standard delivery e Cryostat with encapsulated splash proof micro tome and independent specimen cooling e 5specimen discs 30 mm e 1two parted heat extractor e 1low temperature stabilizer for the heat extractor e 1 cover for quick freezing shelf e 1rimmed section waste tray e 1 storing shelf e 1toolset e 1instruction manual in 4 languages e 1 bottle cryocompound e 1 brush shelf e 2 brushes broad and small e 1 bottle cryostat oil 50 ml e 1 rubber mat e 1 shortinstruction manual e 1 unpacking instruction sheet e 1 dust cover e The rapid sectioning cryostat CM1900 is provided e I transportation crate with a fully encapsulated microtome with an inde e 1 pair of safety gloves pendent specimen cooling system Its graphic control panel is easy to handle due to 5 3 Optional models self explanatory symbols The cryochamber is e The following models are available made of spacious s
25. n the screw 4 e Tighten the screw 4 with an Allen key no 6 To dismount proceed in reverse order 7 4 Locking the handwheel e For locking the handwheel rotate the handle until it is in the upper position Push the locking pin 5 into the recess at the handwheel The locking position is marked by a black dot 6 If necessary move the handwheel slightly forth and back until the locking mechanism engages Leica CM1900 Cryostat 7 5 7 Installation N To unlock push the locking pin 5 to the leftfrom the recess atthe handwheel Mounting the heat extractor Fix the holding device of the heat extractor by tight ening the 2 screws 7 with the Allen key no 4 to the threaded holes on the left sidewall of the cryochamber and insert the heat extractor 7 Installation 7 6 Inserting the accessories e Insert the storing shelf 1 e Insert the rimmed section waste tray 2 18 Instruction manual V5 1 11 2001 7 Installation 7 7 Inserting the optional accessories e Insert the waste container 3 e Insert the right storage shelf 4 e Insert the left storage shelf 5 e Insert the section waste tray 6 Leica CM1900 Cryostat 19 8 Operation 8 1 Precooling the knife 8 2 Installing the knife holder base e To precool the knife put the open knife rest includ e To unlock relocate the lever to the front 1 ing the knife on the storage shelf
26. ns O e The adjustable feet 15 can support the weight of the instrument when tipped at a slight angle max 30 Unscrew the adjustable feet with the open end wrench no 16 when subsequently transporting the instrument any further on wheels do not forget to screw the adjustable feet down completely 7 Installation Transport with handles Transport with a fork lift e For lifting or carrying the instrument manually e g e The instrument can be transported with a fork lift on stairs you can use handles 4 e The distance a for insertion between the wheels Screw the handles into the threads 5 of the bot is max 540 mm tom plate at both sides of the cabinet e The handles can be ordered from your Leica Sales Unit h a 4 Distance for insertion a max 540 mm Due to the weight of the instrument 170 kg 4 To ensure a safe transportation with a fork lift people are required for transportation 2 per 3 people are required one operating the fork sons per handle lift and the other 2 holding the instrument on either side to prevent it from sliding down e Unscrew the adjustable feet atthe installation site 1 with the open end wrench no 16 see Fig page 16 This is absolutely necessary for a stable stand 16 Instruction manual V5 1 11 2001 7 3 Assembly of the handwheel e Insert the pin 1 of the handwheel shaft into the hole 2 of the handwheel e Mount the spring washer 3 o
27. nvoanteas done emneaued aeseeeh ametleviieacsons ans Garatabia aananatennisdaionniaeniuenuatenen 24 D ily operatii scenester tcc ne ER E A AAEE AEE AEAEE EEEE 30 9 1 Selection of the adecuate chamber temperature ccccccscsccssssescsesesssssscsscssesseeecessesesavecsecesscseencessesseseveesevesnsavenensas 30 SVS ZN eana EE N E E 30 9 3 Activating deactivating the specimen cooling s ss sssssssssrsssrururnananurnnunnnnnnananununnnnnnnnanunnrnnnnnnnnananununnnanunananunnnnnnnnnnnnnnnnne 31 94 Activating deactivating the cryochamber cooling cccccccccccssscsscssssssscssesssecessesecescessesessesrsessesereseuseseessesnsensneaseas 31 9 5 Inserting the specimen discs in the specimen head c cccessseesssecsssecsesecsesecessecevsecevsusessesessusavsusessuseusenseseesasensanenseesas 31 9 6 Inserting the knife in the knife Hole ccccccecescsscscsscsessececseseucescessesseseuseseuseusesseseuseusausesseseeseuseuseuseuseusessnsensensansenees 32 97 Moving the specimen towards or away from the knife via coarse feed s sssssssssssssssrsrsrnisinnrnrurininnnnrnrnrnenennrnrernnnns 33 O A a T E A T I 33 9 9 Adjustment of the anti roll guide wo cece cee csscsscsscessesseceseessecsucesseseeseusesseveeseuseuseseuseusesseusesseseusensansessesensensensenseneasenss 34 J10 SS CULO NUM GM receceectr cars saits tn E E E 34 Cleaning and disinfection csiissicsisditecsevseenivivwtassienseinseseeivexacauvsnesauvnnenvsenasinaasdiuviaeaulssnieinenusenieiaedunetaaia
28. of the hinges 2 must point to the in strument 4 Push the lid to the left sliding the jogs 13 of the hinges under the heads of the screws 12 12 Instruction manual V5 1 11 2001 6 Unpacking the instrument Tilt A Lengthwise movement 15 14 Leica CM1900 Cryostat 6 4 Transport to the installation site The instrument must be transported in an up right position or slightly tilted max 30 e The lid 5 forms a ramp for the instrument so it can be rolled off the transport platform Do not grip the cabinet at the lid 16 Grip the cabinet only at the marked locations _ The wheels 14 must be guided over the hinges 2 at the front and rear Caution Risk of tilting 1 Roll the instrument backwards over the ramp off the platform with caution 2 Move the instrument to the installation site on its wheels 3 The adjustable feet 15 can support the weight of the instrument when tipped at a slight angle max 30 7 Installation 7 1 Site requirements To ensure an adequate cooling capacity the instrument must be set up maintaining a mini mum distance from walls and furniture see Technical data The place of installation must meet the following re quirements e No direct sunlight e Mains power socket at a distance no greater than appr 3 m e No drafts air condition outlets etc e Even floor e Vibration free floor e Obstruction fre
29. ohol Dispose of waste liquid according to the waste dis posal regulations Do not use uncontrolled external heaters for drying the cryochamber This can cause damage to the cooling system Do not turn the instrument on before the cryochamber is completely dry Frost formation Dry all parts completely before reinserting them in the cryochamber The front panel and the slit cover of the microtome must be completely dry before turning on the in strument e Prior to removing the microtome turn the instru ment off with the mains switch and pull the mains plug Remove sliding window before removing the micro tome Before removing the microtome lock the handwheel in the lowest position When removing the microtome the specimen head will rapidly fall down and might injure the operator s hands The microtome must be entirely dry before reinstal lation Humidity inside will condense and freeze causing malfunctions and damage All components removed from the cryostat must be carefully dried before returning them to the cryochamber The cooling chamber must be entirely dry when turning on the instrument Frost formation When removing the microtome for cleaning or dis infection please keep in mind the safety instruc tions in chapter 10 Cleaning and Disinfection Instruction manual V5 1 11 2001 3 Safety instructions for handling the instrument Handling samples Defrosting When working wi
30. on of illumination Max Cool button to directly to select the maximum lowest temperature of the specimen head buttons to select specimen head temperature 22 buttons select select real cryochamber time temperature buttons to buttons to select defrost time Key button to lock unlock Melting Snowflake button to activate defrosting display settings and to turn on off chamber or specimen cooling Instruction manual V5 1 11 2001 8 Operation Luminous indication LED flashes while coarse feed is in motion and and lights up when the specimen head has reached its rear limit position Coarse Feed buttons Arrow buttons on the left of the cryochamber To move the specimen head rapidly towards the knife lower button or away from the knife upper button Luminous indication LED lights up when the specimen head has reached its front limit position Mounting the knife holder onto the knife holder base Quick freezing shelf with heat extractor and low temperature stabilizer Leica CM1900 Cryostat 23 8 Operation 8 7 Programming the desired values Turning the lamp on and off e Turn the lamp on or off Setting the time e Setthe time HH HH e The actual time is set with the buttons in the control panel marked with the clock symbol 9 ue When pushing the or button for more than 1 s the time value increases or dec
31. ours once the chamber temperature varies by less than 5 K from the set temperature If you want to turn the specimen cooling back on manually before the automatic activation sets in push the or button on the panel for the speci men cooling and then the key button The temperature of the specimen cooling first raises to 10 C and then adjusts to the programmed speci men temperature Instruction manual V5 1 11 2001 8 Operation Leica CM1900 Cryostat Before defrosting the cryochamber remove all samples Manual defrosting of the cryochamber Push the manual defrost button with the melting snowflake on the left over the key button to acti vate the defrost cycle of the cryochamber on de mand Activation is confirmed by an audible signal Then push or button on the panel for the cryochamber temperature The manual defrost cycle 9 min is activated There is a flashing indication of the temperature of the cryochamber during the whole defrost cycle lf necessary push the manual defrost button on the panel for the cryochamber temperature When activating the manual defrost cycle the speci men head temperature adjusts to a temperature be tween 10 C and 5 C reduced ice formation The specimen head cooling turns off This is confirmed by the flashing of the decimal points on the panel for the specimen cooling Ten seconds after the manual defrost cycle has been completed the specimen coolin
32. pecimen detached from disc after freezing Blunt knife Inappropriate section thickness Incorrect clearance angle Microtome not properly dry before reinstallation Dried specimen Anti roll plate is too warm or incorrectly positioned Fat on the corner or edge of anti roll plate Anti roll plate not correctly fixed Rust on the knife Anti roll plate too warm Temperature too low for the tissue cut Blunt part dirt dust frost or rust on the knife Top edge of the anti roll plate damaged Hard particles in the tissue Dirt on knife back Microtome was not entirely dry when switching refrigeration which produced ice built up in the micrometer feed system Microtome defective Remedies Replace anti roll plate Be more careful next time Select correct temperature Use knife with different profile c or d Remove Ice Adapt speed Check knife clamping Check disc clamping Apply cryocompound to warm disc mount specimen and freeze Use different part od the knife edge or replace knife Select correct section thickness Set correct angle Dry microtome thoroughly Prepare new specimen Cool down anti roll plate or reposition correctly Remove fat from anti roll plate Fix correctly Remove rust Cool down anti roll plate Increase temperature and wait Remove cause Replace anti roll plate Clean Remove microtome and dry thoroughly Call technical service 43
33. quality control procedure based on the Leica in house testing standards and that the product is faultless and complies with all technical specifications and or characteristics warranted The warranty conditions depend on the contents of the individual contract concluded supplemented by the warranty conditions of your local Leica sales unit The warranty period starts on the day of passing of risk or on the day of delivering the product Excluded are warranties for defects or damage attrib utable to for example operational and normal wear and tear improper use faulty operation installation and or use of non original spare parts installation and or use of non original accessories negligent handling of the product by the customer connection to unsuit able power supply sources operation at incorrect volt age force majeure such as fire lightning earthquakes humidity etc We will not assume any liability for damages due to im proper handling of the product Any repairs and or exchange of parts of the product must be carried out by technical service engineers authorized by Leica Otherwise warranty claims can no longer be made The local Leica sales unit the Leica representative re sponsible or the factory in Nussloch must be consulted prior to any changes to the instrument to any modifica tions as well as prior to any use of the instrumentin com bination with non Leica components not expressly au thorized by Leica Spare
34. r messages The display indicates the following malfunctions Error code E 001 Temperature limit error or breaking of the tempera ture sensor of the specimen cooling system Error code E 011 Temperature limit error or breaking of the tempera P AAS ture sensor of the cryochamber cooling system Ifthe instrument is being exposed to temperatures of more than 35 C over a long period e g during transportation a temperature limit error may be in dicated This error message disappears as soon as the In strumenthas reached a temperature lower than 35 a lf the error message does not disappear call the technical service doge Never leave samples in the cryochamber The instrument is not made for storing frozen specimen Unfixed samples used in cryostats must al ways be considered as possibly contami nated Ensure that the appropriate disinfec tion methods are applied see Cleaning and Disinfection Leica CM1900 Cryostat 29 9 Daily operation 9 1 Selection of the adecuate chamber temperature For choosing the adecuate chamber temperature see the temperature chart on page 50 92 Specimen freezing 30 Freeze the specimen on the quick freeze shelf onto the specimen disc Place the heat extractor onto the specimen to speed up the freezing process Rotate the heat extractor to place it on the three freezing stations within reach To place the heat extractor onto one of th
35. ransparent sheet attached to the transport crate at arrival of the shipment The following unpacking instructions shall help you in case of packing or transporting the instrument once again 6 1 6 1 How to open the crate 1 Loosen 3 screws 1 at the top of the two sides of the crate 7 2 Loosen 4 screws of the hinges 2 at the front and rear of the crate Do not loosen the screws of the hinges on the lid of the crate 3 Loosen 1 screw 3 at the top between the two hinges at the front and rear of the crate 4 Loosen 3 screws 4 at the bottom on each of the four sides of the crate rr Se 6 2 How to remove the packing materia in i 1 Remove the lid 5 and place on the ground beside the transport platform 11 with the foam parts fac ing down 2 Remove the 4 foam parts 6 7 9 4 Take out the cardboard box 8 containing the ac cessories and the instruction manual atthe front of the instrument 10 3 Liftthe body ofthe crate 7 straight up i 5 Remove the dust cover 9 by pulling it upward l 6 Remove the foam part 10 at the rear 11 Leica CM1900 Cryostat 11 6 Unpacking the instrument 11 6 3 Mounting the ramp 1 Upon removal of the foam part 10 2 screws 12 become visible on the base platform 2 Fold down the hinges 2 at the rear and front 3 Place the lid against the transport platform 11 from behind The jogs 13
36. reases continu ously Autorepeat function Programming the temperature of the cryochamber e Selectthe desired temperature of the cryochamber e The temperature of the cryochamber is set and in dicated on the control panel marked with the cry ostat symbol The actual temperature Is the standard indication For indication of the desired value touch the or button Set the desired value via the buttons When pushing the or button for more than 1 s the cham ber temperature value increases or decreases continuously The actual value will be indicated 5 seconds after finishing the programming 24 Instruction manual V5 1 11 2001 8 Operation Programming the specimen temperature e Select the desired temperature of the specimen e The specimen temperature Is set and indicated on the control panel marked with the specimen head symbol The actual temperature Is the standard indication For indication of the desired value touch the or button Set the desired value via the buttons When pushing the or button for more than 1 s the speci men temperature value increases or decreases continuously The actual value will be indicated 5 seconds after finishing the programming Specimen temperature Max Cool function e The snowflake button for the Max Cool function is in the panel with the specimen head symbol Push the button for programming the lowest tem perature possible of the
37. rth e The chamber cooling is activated by subsequently pushing the or button in the control panel for e Deactivation of the chamber cooling is done in the same way e Insert the specimen disc in the specimen head e Loosen the screw 1 onthe specimen head turning it counterclockwise Insert the specimen disc and retighten the screw Leica CM1900 Cryostat 31 9 Daily operation 9 6 Inserting the knife in the knife holder e Insert and clamp the precooled knife blade in the knife holder For further details please refer to the separate in struction manual for your knife holder A 32 Take care when handling microtome knives and disposable blades The cutting edge is extremely sharp an can cause severe injury Never leave knives and knife holders with a knife blade mounted lying around Do not place a knife on a table with the cut ting edge facing upward Never try to catch a falling knife Prior to manipulating the knife and specimen or changing the specimen or knife always lock the handwheel and cover the cutting edge with the knife guard Always lock the handwheel and cover the cutting edge of the knife with the knife guard during breaks Avoid contact with cold parts of the instrument as this can cause frostbite Instruction manual V5 1 11 2001 9 Daily operation 9 7 Moving the specimen towards or away from the knife via coarse feed e Push the coarse feed button
38. s Reposition anti roll plate Align correctly Set correct angle Use different part of the knife or replace the knife Clean with dry cloth or brush Replace anti roll plate Use different part of the knife or replace the knife Readjust correctly Readjust correctly Use different part of the knife or replace the knife Replace anti roll plate Refreeze specimen onto the disc Check disc clamping Check knife clamping Refreeze specimen onto the disc Increase section thickness reduce specimen surface area if necessary Use different part of the knife or replace knife Use knife with differnt profile Set correct angle Store all tools on storage shelf in the cryochamber Instruction manual V5 1 11 2001 14 Troubleshooting Problem Anti roll plate damaged after adjustment Thick thin sections Tissue sticks or crumbles on the anti roll plate Flattened sections curl up when anti roll late is picked up Sections tear Inconsistent or insufficient specimen feed Leica CM1900 Cryostat Causes Anti roll plate too high above the knife edge The adjustment was carried out in the direction of the knife Temperature incorrect for the tissue cut Knife profile inappropriate for the specimen cut Ice buildup in the knife back Handwheel speed not uniform Knife not clamped tightly enough Specimen disc not clamped tightly enough Cryocompound applied to cold specimen disc s
39. s situated on the left upper side of the cabinet to move the specimen towards or away from the knife The upper coarse feed button automatically re tracts the specimen from the knife the lower but ton moves the specimen toward the knife e Only touch the upper coarse feed button slightly to retract the specimen from the knife The specimen cylinder automatically moves to the rear limit The return movement can be stopped by pressing one of the coarse feed buttons e The forward movementonly operates as long as the button is pressed This is a safety feature to pro tect both specimen and knife from damage Always observe the specimen during the for ward movement thus avoiding that specimen and knife collide by mistake A collision can cause severe damage both to the knife and to the specimen and the speci men disc Leica CM1900 Cryostat 98 Trimming e When the specimen has been moved towards the knife it can be trimmed to the required sectioning plane The section thickness can be adjusted by turning the knob on the right beside the specimen cylin der 10 0 20 60 30 SO 40 e Start sectioning after having finished trimming or having reached the appropriate section thickness 33 9 Daily operation 9 9 Adjustment of the anti roll guide A 34 Adjust the anti roll guide to the knife holder before sectioning On both sides of the anti roll plate the profiles of the individ
40. s room temperature The microtome is encapsulated splash proof inthe cryochamber Thus it is not necessary to remove it for disinfection Spray disinfection Is possible Before disinfecting put an appropriate vessel un derneath the marked hose connection on the right hand side of the cabinet see below Drain the cleaning liquid through the hose after the prescribed reaction time by pulling the stopper Dispose of the waste liquid according to the waste disposal regulations 35 11 Removal of the microtome 11 1 How to remove the microtome 2 Loosen the specimen head turning the Allen key no 5 counterclockwise Prior to removing the microtome turn the in strument off with the mains switch and pull the mains plug Remove the sliding window before removing the microtome Before removing the microtome lock the handwheel in the lowest position When re moving the microtome the specimen head will rapidly fall down and might injure the operator s hands 1 Remove the section waste tray 3 Pulloutthe specimen head carefully and place iton the storage shelf which Is on the left of the micro tome 36 Instruction manual V5 1 11 2001 11 Removal of the microtome 4 Hold the front panel of the microtome as shown 6 Loosen the central screw with the Allen key no 5 seizing the border with your lefthand and the open ing with your right hand and take it out 5 T
41. specimen head 50 C e Push the snowflake button again for stopping the Max Cool function The temperature adjusts to the value programmed prior to activating the Max Cool function e Alternate flashing of LL and actual temperature indicates activation of the Max Cool function Leica CM1900 Cryostat 25 Operation 26 cHHLHH doge Programming the defrost cycle Set the beginning of the automatic defrost cycle The automatic defrost cycle is activated once in 24 hours Itis set with the buttons on the right of the panel with the clock symbol The buttons are marked by a melting snowflake Touch the or button for indication of the begin ning of the defrost cycle which has actually been set Atthe same time the LEDs between the indica tion of hours and minutes are flashing To change the beginning of the defrost cycle in steps of 15 minutes push the or button When pushing the or button for more than 1 s the de frost time value increases or decreases continu ously Before starting the defrost cycle remove all samples from the cryochamber When activating the automatic defrost cycle the specimen head temperature adjusts to a tempera ture between 10 C and 5 C reduced ice forma tion The specimen head cooling turns off This is confirmed by the flashing of the decimal points on the panel for the specimen cooling The specimen cooling turns automatically back on after 4 h
42. tainless steel which Is easy to clean e CM1900 with specimen retraction The instrument is CFC free and is designed and e CM1900 without specimen retraction manufactured according to VDE and UL regulations Please indicate type when ordering 5 1 Designed use improper handling e Samples must not be stored unattended in the cry 9 4 Optional accessories ostatfor along period since they could be destroyed e orienting specimen head in case of a power failure or malfunction of the in e thermal block strument e storage set including waste container consisting of The cryochamber temperature can rise consider e storage shelf left ably during defrosting Heat sensitive samples must e storage shelf right therefore be protected from exposure to elevated e waste container temperatures and removed prior to defrosting e section waste tray The instrument may only be used for the specified application and operated in accordance with the instructions given in this manual Any other use of this instrument is considered as improper operation 10 Instruction manual V5 1 11 2001 6 Unpacking the instrument Checking if the instrument has been transported correctly Please check the TIP N TELL indicators A immediately after arrival of the shipment If the TIP N TELL Arrow Point is blue this package has been on its side or tipped in transit Please note on the Bill of Lading and check for damage The same unpacking instructions are inside a t
43. th possibly contaminated or in fected material the general safety guidelines for laboratories must be applied Before defrosting the cryochamber remove all samples Before defrosting the specimen head remove all samples Never leave samples in the cryochamber The in strument is not made for storing frozen specimen Leica CM1900 Cryostat Maintenance Turn the instrument off with the mains switch and pull the mains plug before replacing the fuses Only use fuses of the same specification For the required values refer to chapter 4 Technical Data Turn the instrument off with the mains switch and pull the mains plug before replacing the lamp Ifthe lamp is broken it must be replaced by the tech nical service as the replacement involves a high risk of Injury Only use lamps of the same specification For the required type refer to chapter 4 Technical Data 4 Technical data Type Certificate of conformity Nominal voltage Nominal frequency Power draw Max start up current for 5 sec Protective class Mains fuses MDA by Bussmann Automatic fuse Pollution degree Overvoltage installation category Heat emission max 230 V AC 50 Hz 1800 VA 25 A eff T8A T10A T1 2 lI 1800 J s 200 V AC 50 Hz 1800 VA 30A eff T12A T12A T1 2 lI 1800 J s UL CSA 120 V AC 60 Hz 1800 VA 35 A eff T15A T1 2 lI 1800 J s 200 V AC 60 Hz 1800 VA 30 A eff T15A
44. ual knife types are engraved C stands for knife profile c D for knife profile d and E for disposable blades The corresponding letter on the upper side of the anti roll plate pointing towards the user must not be inverted Adjust the anti roll guide correctly For further details of adjusting the anti roll guide please refer to the instruction manual for knife hold ers Attention Only use acetone for cleaning the plastic anti roll plate of the knife holders CN and CS The glass anti roll plate of the knife holder CE can be cleaned either with acetone or alcohol Applies only for knife holders CN and CS 9 10 Sectioning When sectioning make sure that the section smoothly slides down between the anti roll plate and the knife or the pressure plate of the knife holder There are two different methods of taking sections 1 Applying the section onto a warm slide Fold back the anti roll plate and carefully approach a slide of room temperature to the section The section flies onto the slide and adheres tightly to the slides surface Unfortunately itis not possible to orientate the sec tion subsequently on the slide 2 Applying the section onto a precooled slide Place the section with a smooth brush on a slide which has been cooled down to the temperature of the cryochamber Unfortunately the section does not lay smoothly on the slide and must be smoothed with a brush When using a precooled slide alwa
45. ys warm it with your hand from the bottom side after the specimen has been placed smoothly on it thus making sure that the section adheres tightly to it and does not slide down when going on with preparation e g staining Instruction manual V5 1 11 2001 10 Cleaning and disinfection 10 1 Cleaning and disinfection The microtome is encapsulated splash proof in the cryochamber Thus spray disinfection with Leica Cryofect is possible When disinfecting please take appropriate protective measures gloves mask protective clothing etc When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer Only use acetone for cleaning the plastic anti roll plate of the knife holders CN and CS The glass anti roll plate of the knife holder CE can be cleaned either with acetone or alcohol Do not use uncontrolled external heaters for drying the cryochamber This can cause dam age to the cooling system 10 2 Turning the instrument back on Do not turn the instrument on before the cryochamber ts completely dry Frost formation The front panel and the slit cover of the mi crotome must be completely dry before turn ing on the instrument Dry all parts completely before reinserting them in the cryochamber e The stopper must be plugged to the hose again Leica CM1900 Cryostat Turn off the instrument and wait until the cryochamber ha
46. zen specimen from the specimen disc e Place the cap 2 on the required side so that the appropriate location hole for the specimen disc Is visible e Insert the shaft of the specimen disc in the appro priate location hole 3 at the top or bottom of the thermal block e After about 20 seconds the frozen specimen can be removed from the specimen disc with forceps e f the cap is too loose readjust it with the small screw 4 Do not overtighten the screw e Once the specimen is removed take the thermal block out of the cold cryochamber Keep the thermal block outside the cryochamber at room temperature Place it in the cryochamber only for specimen removal Leica CM1900 Cryostat 47 17 Ordering information Ordering information Knife holder Knife holder CN as above but without knife holder base Knife holder CE for low profile blades with glass anti roll guide Knife holder CE for high profile blades with glass anti roll guide Pressure plate for high profile blades for Cat no 0419 30383 Pressure plate for low profile blades for Cat no 0419 30390 Knife holder base for knife holders CN CE and CS Knife holder CS without knife holder base Auxiliary clamping device for knife holder CS Replacement knife guard Knife support bar Magnetic blade holder for Cryolap disposable blades Specimen discs Specimen disc 20 mm g Specimen disc 25 mm g Specimen disc 30 mm g Specimen disc 40 mm g Specimen disc 55mm
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