SERVICE MANUAL
Contents
1. 100 CONTROLBUTTONLATER I E CHANGE THE WAVELENGTH 36 16 C Analyze Sample 8 RINSE A SECOND CUVETTEWITHA SMALLAMOUNT OF THE SAMPLE SOLUTION TOBE TESTED FILL THE CUVETTEHALFFULLAND WIPE IT 9 PUT THE SAMPLE CUVETTEIN THE SAMPLE COMPARTMENT CLOSE THE LID 10 READ THE TRANSMITTANCE ORABSORBANCE FROM THE DIGITALREADOUTWINDOW REMOVE THE SAMPLE CUVETTEORTEST TUBE 11 IF YOU ARE TOTESTTHE SAME SAMPLE AT OTHERWAVELENGTHS REPEATSTEPS 3 TO 10 FOR EACH WAVELENGTH 12 FOREACH NEW SAMPLE YOU ANALYZE REPEATSTEPS 2 TO 11 Additional Features of Model 1100RS ALTHOUGH OPERATING BASICALLYTHE SAME AS THE MODEL 1100 THE 1 100RS SPECTROPHOTOMETER OFFERSTWO MORE FEATURES CONCENTRATION MODE AND FACTORMODE AS FOLLOWS Concentration C Mode for determining the concentration of unknown samples NOTE This method should only be used when the relationship between Absorbance and Concentration is known to be linear The concentration of the Standard solution used to calibrate the instrument should be higher than the most concentrated sample SELECTTHE DESIRED WAVELENGTH BY TURNING THE WAVELENGTH CONTROLKNOB USING THE MODE BUTTON SELECTABSORBANCE A MODE INSERTTHE CUVETTECONTAINING THE BLANK SOLUTION SET O 000A WITH THE 0A 100 T CONTROLBUTTON USING THE MODE BUTTON SELECTC CONCENTRATION MODE INSERTA CUVETTECONTAINING A STANDARD SOLUTION OF KNOWN CONCENTRATIONIN THE SAMPLE COMPARTMENTAND ADJUSTTHE DIGITALREADOUTWIND2. UNICO 1100 SERIES SPECTROPHOTOMETER Model 1100 Spectrophotometer Model 1100RS Spectrophotometer UNITED PRODUCTS amp INSTRUMENTS INC e MANUFACTURER OF QUALITY OPTICAL SYSTEMS CONTENTS 1 GENERAL 1 1 Introduction 1 2 Optical System 1 3 Electronic 1 4 Specifications 1 5 Nomenclature 2 INSTALLATION 3 MAINTENANCE 3 1 Working Environment 3 2 Replacing Lamps And Adjustments 3 3 Wavelength Calibration 4 SERVICE 4 1 Operating Procedure Check 4 2 Physical 4 3 Troubleshooting 5 DRAWING AND DIAGRAM 5 1 PCB Arrangement of Synthetic Electronic System for UNICO 1100 5 1 1 Circuit Diagram of Synthetic Electronic System for UNICO 1100 5 2 PCB Arrangement of 115v 49v Power Supply 5 2 1 Circuit Diagram of 115v 49v Power Supply 5 3 PCB Arrangement of W Lamp Power Supply 5 3 1 Circuit Diagram of W Lamp Power Supply 5 4 PCB Arrangement of Logarithm Adjustment 5 4 1 Circuit Diagram of Logarithm Adjustment 5 5 Arrangement of 0 Adjustment 5 5 1 Circuit Diagram of 0 T Adjustment 5 6 PCB Arrangement of Amplifier 5 6 1 Circuit Diagram of Amplifier 6 SCHEMATIC LOYOUT 6 1 Schematic Diagram of UNICO 7 ELECTRONIC PARTS FOR UNICO 1100 7 1 Electronic Parts list for UNICO 1100 09 1 GENERAL 1 1 INTRODUCTION The UNICO model 1100 Series spectrophotometers are single beam general purpose instruments designed for use in conventional laboratories
3. Power Switch Power Socket 1100RS UNICO 1100 SPECTROPHOTOMETER AND NOMENCLATURE 2 INSTALLATION 2 1 The instrument should be installed in a clean environment which is free of corrosive materials dust and severe vibrations Furthermore it should be kept away from any strong electric magnetic fields and high frequency fields 2 2 LINE VOLTAGE The power can be selected by means of the built in switchable power switch to match the line voltage in your country 36 10 3 MAINTENANCE 3 1 WORKING ENVIRONMENT The optimum environment for the instruments is as follows TEMPERATURE 5 35 HUMIDITY 85 Install the instrument in a clean non vibrational area Avoid direct strong light and air flow The instrument will give better performance if the power supply stability is at 1 and line frequency less than 1Hz Always allow the instrument to warm up for 20 minutes prior to taking any readings 3 2 REPLACING LAMPS AND ADJUSTMENTS Turn off and unplug the instrument Turn the instrument upside down Remove the grill plate on bottom of the instrument by removing fixing Screw Unplug the lamp from the white connector Insert the new lamp pushing it in as far as it will go The lamp filament should face the center of the collecting lens CAUTION DO NOT HANDLE HALOGEN LAMP WITH BARK FINGERS USE TISSUE OR CLOTH WHEN HANDLING LAMP 8 Reinstall the grill plate com Figure 3 1 Terminal
4. The instruments are ideal for various applications such Clinical Chemistries Biochemistry Food and Beverage Laboratories Environmental Protection Water and Waste Water Labs and other fields of quality control The UNICO 1100 Series spectrophotometers cover the visible wavelength range from 335nm to 1000nm The analytical grating system has 1200 lines mm for higher dispersion of the spectrum 36 3 Figure 1 1 1 UNICO Model 1100 Spectrophotometer Figure 1 1 2 UNICO Model 1100RS Spectrophotometer 36 4 1 2 OPTICAL SYSTEM As shown in Figure 1 2 the white light coming from the Halogen Lamp is focused by the collecting lens pass through the Entrance slit of the Monochromator through a collimating lens to the Analytical grating where it is dispersed into its spectrum then through the Exit slit of the monochromator continues through the Sample compartment and finally to the Photo detector Figure 1 2 OPTICAL SYSTEM SCHEMATIC DIAGRAM 1 Halogen Lamp 2 Collecting Lens 3 Entrance Slit 4 Collimating Lens 5 Grating 6 Exit Slit 7 Sample 8 Collimating Lens 9 Photodiode 36 5 1 3 ELECTRONIC SYSTEM The photo detector receives the monochromatic light and generates photocurrent A direct current signal which is proportional to the amount of light energy to the photo detector The signal is then amplified and processed The processed signal is sent to the digital display and also to the exter
5. and W lamp power supply Cuvette in position q N Y Install cell holder cuvette N Repair shutter Shutter operation Y Adjust the lamp position keep shutter open put a white paper Halogen lamp peaked F ppp into optical path in sample comparment at 525nm you should see bright green light Y on paper Y na A check S1227K photocell Amplifier detector 122 p B refer Fig 5 3 1 to check Working fine amplifier C solving wiring problem 36 23 TROUBLE SHOOTING Problem Turn on put in cell holder and cuvette display lt lt 10 0 press 10096T display not change Is the position of halogen lamp Y Adjust the position of halogen lamp put a piece of Is the light road out of white retangle paper into the position sample comparment open the shutter can see a whole clear bright facula Y Right filter in position N Adjust light road with right way put apiece of white retangle paper open the shutter can see a whole clear bright facula Adjust the filter 36 24 TROUBLE SHOOTING 4 Problem Instrument on Display 1 T or show negative at absorbance Y Is sample cover closed compartment N y Is cell holder and cuvette in p
6. DISPLAY 6 ADVANCE THE WAVELENGTHSETTING BY 1NM AND REPEATSTEPS 4 TO 7 7 REPEATSTEP 8 UNTILTHE WAVELENGTHSETTING REACHES 370NM 8 LOOKFORTHEMAXIMUM ABSORBANCE READING OBTAINED AND THIS SHOULDBE FOUND BETWEEN 359 AND 363NM THE WAVELENGTH ACCURACY OF THE 1 100 1 10065 IS 2NM Didymium Filter Method 1 SETTHE WAVELENGTH TO800 NM 2 MAKE SURE THE CUVETTEHOLDERIS EMPTY AND PLACE ITIN THE SAMPLE COMPARTMENT CLOSE THE SAMPLE COMPARTMENT LID 3 SETZEROABS BY PRESSING THEOA 100 T FEWSECONDS WHILE THE DISPLAY FLASHES BLA THE READING SHOULDTHEN BE 0 000A IF NOT REPEATSTEP 36 12 4 REMOVE THE CUVETTEHOLDERAND INSERTTHE DIDYMIUM FILTERINTOIT PLACE ITIN THE SAMPLE COMPARTMENTAND CLOSE THE LID 5 RECORDTHE ABSORBANCE READING ON THE DIGITALDISPLAY 6 ADVANCE THE WAVELENGTHSETTING BY 1NM AND REPEATSTEPS 2 TO5 7 REPEAT STEP 6 UNTILTHE WAVELENGTHSETTING REACHES 8 15NM 8 LOOKFORTHEMAXIMUM ABSORBANCE READING OBTAINED AND THIS SHOULDBE FOUND BETWEEN 805 AND 809NM THE WAVELENGTH ACCURACY OF THE 1 100 1 10085 15 2NM 9 IFA MIDDLE WAVELENGTHCHECK IS DESIRED SET THE WAVELENGTH TO 522NM OPTIONAL 10 MAKE SURE THE CUVETTEHOLDERIS EMPTY AND PLACE ITIN THE SAMPLE COMPARTMENT CLOSE THE SAMPLE LID 11 SETZEROABS BY PRESSING THE OA 10096T BUTTON WAITA FEW SECONDS WHILE THE DISPLAY FLASHES BLA THE READING SHOULDTHEN BE IF NOTREPEATSTEP 1 1 12 REMOVE THE CUVETTEHOLDERAND INSERTTHE DI
7. DYMIUM FILTERINTOIT PLACE ITIN THESAMPLE COMPARTMENTAND CLOSE THE LID 13 RECORD THE ABSORBANCE READING ON THE DIGITALDISPLAY 14 ADVANCE THE WAVELENGTHSETTING BY 1NM AND REPEATSTEPS 10 TO 13 REPEAT STEP 14 UNTILTHE WAVELENGTH SETTING REACHES 536NM AGAIN LOOK FORTHE MAXIMUM ABSORBANCE READING ITSHOULDBE BETWEEN 527 AND 531NM Absorbance Accuracy Checks SPECIFICATION 2 AT 1A 1100 1 AND 2A 1100RS THE ABSORBANCE ACCURACY SHOULD BE CHECKED AGAINST A SET OF NEUTRALDENSITY FILTERS ACCURATELY CALIBRATED TO THE NIST STANDARDS CONTACTYOURUNICO REPRESENTATIVE FORMORE INFORMATION 800 588 9776 AN ALTERNATIVE METHOD USING POTASSIUM DICHROMATE IS DESCRIBED BELOW DUE TOTHE MANY FACTORSTHATMIGHT AFFECTTHE RESULTS I E TEMPERATURE BANDPASS WEIGHING AND DILUTING ERRORS THIS METHOD IS LESS ACCURATEAND SHOULDONLY BE USED AS A GUIDE REFERENCE JOHNSON E A POTASSIUM DICHROMATE AS AN ABSORBANCE STANDARD PSG BULLETIN1967 NO 17 PAGE 505 36 13 MAKE UP N 100 SULFURICACID AS THE SOLVENTAND USE PARTOFITTOMAKE A SOLUTION CONTAINING 120 0 5MG LITREOF POTASSIUM DICHROMATE WASH SQUARE CUVETTEWITH SOLVENT AND FILLWITH SOLVENT PUT THE CUVETTEIN THE ADAPTERINTO THE SAMPLE COMPARTMENT AND CLOSE THE LID SET THE WAVELENGTHTO 350NM SET THEMODE BUTTONTOA SET THE READING USING THE OA 10096T BUTTON EMPTY THE CELL WASH OUTWITH DICHROMATE SOLUTION AND FILLWITH DICHROMATE SOLUTION PUT TH
8. E CUVETTEIN THE ADAPTERINTO THE SAMPLE COMPARTMENTAND CLOSE THE LID READ THE ABSORBANCE OF THE STANDARD FROM THE DISPLAY THE VALUE SHOULDBE 1 288 0 04A REFERTO THE NOTES ABOVE WHEN INTERPRETING THE RESULT 36 14 Stray Light Check SPECIFICATION LESS THAN 5 AT340NM BY ASTM E 387 GOOD INDICATION AS TO WHETHERTHE STRAY LIGHTLEVELIS WITHIN SPECIFICATION MAY BE OBTAINED AS FOLLOWS 1 SET THE WAVELENGTHTO 340NM 2 SET THE SWITCH TO96T 3 WITH THE SQUARE CELLADAPTERIN THE SAMPLE COMPARTMENT BUT NO CELL CLOSE THE LID AND PRESS THE 100 BUTTONTOSET THE DISPLAY TO 100 096 4 REMOVE THE CELLADAPTER FROM THE SAMPLE COMPARTMENT AND CLOSE THE LID MAKE A NOTE OF THE READING THATSHOULDBE AT ORNEAROO O 5 PREPARE A SOLUTION CONTAINING 50GM L OFSODIUM NITRITE NANO 2 IN DISTILLEDWATERAND FILLA SQUARE CUVETTEWITH THIS SOLUTION 6 INSERTTHE CUVETTEINTO THE ADAPTERAND PLACE IN THE SAMPLE COMPARTMENT CLOSE THE LID THE DISPLAY SHOULDREAD O 596T NOTE THATIF THE READING OBTAINED IN STEP 4 IS GREATERTHAN OO O THIS VALUE SHOULD BE SUBTRACTED FROM THE DISPLAYED READING TOGIVE THE CORRECTREADING FORTHE STRAY LIGHT VALUE 36 15 SERIVCE 4 1 PHYSICAL INSPECTION Thoroughly inspect the instrument for physical indications that might cause a malfunction The following categories are usually checked Apparent damage Dirty Optics Broken wire loose connection P C Board loose Broken lands on pc board any sign of
9. Lamp Socket Halogen Lamp Lamp holder Replacing the lamp 3 3 Wavelength Calibration 36 11 NORMALLYTHE UNICO 1100 SERIES SPECTROPHOTOMETERRETAINS ITS WAVELENGTH CALIBRATION INDEFINITELY HOWEVERIF THE INSTRUMENT RECEIVES A SEVERE SHOCK ORIS ABUSED USE THE FOLLOWING METHODS TOCHECK WAVELENGTHCALIBRATION PLEASE NOTE THATTHIS TEST REQUIRES THE UNICO DIDYMIUM FILTERP N 1100 110 ORTHE HOLMIUM OXIDE FILTER P N 1100 109 IN THE FILTERM ETHOD THE DIDYMIUM FILTERHAS TWO DISTINCTABSORBANCE PEAKS 529NM AND 807NM THE HOLMIUM FILTERHAS A DISTINCTPEAK AT361NM WHEN THE INSTRUMENTIS CALIBRATED PROPERLY YOU WILLFIND MINIMUM TRANSMITTANCE MAXIMUM ABSORBANCE AT THE RANGE 2 FROM THESE PEAKS NOTE THATTHE SPECIFIC TRANSMITTANCE VALUES ARE NOTIMPORTANTAS YOU ARE ONLY LOOKING FORTHE WAVELENGTH WHERE THE MINIMUM TRANSMITTANCE MAXIMUM ABSORBANCE OCCURS Holmium Oxide Filter Method 1 TURNINSTRUMENTON AND ALLOWIT TO WARM UP FOR15 MINUTES 2 SELECTTHE ABSORBANCE OPERATING MODE 1 SETTHEWAVELENGTHTO350NM 2 MAKE SURE THE CUVETTEHOLDERIS EMPTY AND PLACE ITIN THE SAMPLE COMPARTMENT CLOSE THESAMPLE COMPARTMENT LID 3 SET ZERO ABSORBANCE BY PRESSING THE OA 10096T WAIT A FEW SECONDS WHILE THE DISPLAY FLASHES BLA THE READING SHOULDTHEN BE 0 000A IF NOT REPEATSTEP 5 4 REMOVE THE CUVETTEHOLDERAND INSERTTHE HOLMIUM FILTERINTOIT PLACE ITIN THE SAMPLE COMPARTMENTAND CLOSE THE LID 5 RECORDTHE ABSORBANCE READING ON THE DIGITAL
10. NDOW TO THE DESIRED VALUE OF THE MULTIPLIER 4 PRESS THE ENT BUTTON THE SPECTROPHOTOMETERSWITCHES TO THE CONCENTRATION C MODE Operational Note If the Concentration of the sample is too high to be displayed the instrument will not switch to Concentration Mode when the ENT button is pressed Dilute the sample and multiply the concentration reading by the dilution factor to obtain the original sample concentration 5 READ THE CONCENTRATION VALUE OF THE SAMPLE DIRECTFROM THE DISPLAY 6 INSERTA CUVETTECONTAINING THE NEXTSAMPLE AND READ THE RESULT REPEAT UNTILALL SAMPLES HAVE BEEN MEASURED Operational Note If the MODE switch is changed to A or T then the concentration reading is frozen This requires the operator to re start at step 1 36 18 MODE Button Mode Indicator 0A 100 T T A Selects T Abs Button OPERATION INSTRUCTIONS 0A 100 T O UNICO 1100 SPECTROPHOTOMETER Sample Compartment Wavelength Control for 10mm Test Tubes Knob WAVELENGTH or 10mm Square and Wavelength Readout Cuvettes Model 1100 Spectrophotometer MODE INDICATOR Allows the operator to know the measurement mode currently in use Transmittance Absorbance MODE BUTTON Switches between Transmittance and Absorbance operating modes at any time of measurement OA 10096T BUTTON Adjusts digital readout to 100 T or 0 000A when blank reference solution is in sample compartment SAMPLE COMPARTMENT Insert 10mm test tube an
11. OW TO THE VALUE OF THE STANDARD BY USING THE A AND V ARROWS 7 PRESS THE ENTERBUTTON o BRON NOTE If the reading changes the factor required is too high i e gt 1999 to be displayed In this case divide the concentration by 10 re select the C mode by successive presses on the Mode button cycling through the F and A modes and follow step 2 above to set the concentration of the standard to the reduced value 36 17 8 WITH THESTANDARD CONCENTRATIONSET DETERMINE THE CONCENTRATION VALUES OF SAMPLES WITH UNKNOWN CONCENTRATION BY INSERTING THE SAMPLE CUVETTEINTO THE SAM PLE COMPARTMENT AND READING THE VALUE DIRECTFROM THE DISPLAY 9 TO READ THE VALUE OF THE MULTIPLIERUSED TOCONVERTABS TO CONCENTRATION EASURING ALLTHE SAMPLES CHANGE THE MODE TOFACTOR F AND READ THE MULTIPLIERFROM THE DISPLAY KEEP A RECORDOF THIS VALUE FORFUTUREUSE Operational Note if the mode switch is changed to read Factor or Absorbance the Concentration C reading is frozen and cannot be changed This requires the operator to re start at step 1 Factor F Mode THIS IS A SPECIALMODE FORMEASURING CONCENTRATION VALUES OF UNKNOWN SAMPLES USING A PREVIOUSLY DETERMINED FACTORTO CONVERTABSORBANCE READINGS TO CONCENTRATION 1 AFTERSETTING THE WAVELENGTH AND SETTING ZERO ABS ON THE BLANK SOLUTION USING THE MODE BUTTON SELECTFACTOR F MODE 2 INSERTA CUVETTECONTAINING A SAMPLE 3 USING THE AAND V ARROWS SET THE DIGITALREAD OUT WI
12. d close lid or fit 10mm cuvette adapter and fit adapter with holder to left of cuvette into compartment WAVELENGTHCONTROLKNOB WAVELENGTH Selects desired wavelength in nanometers nm WAVELENGTH READOUT WINDOW Displays desired wavelength 36 19 MODE 0A 100 T 0 T Button Button Mode Indicator Button Print ENT Button OPERATION INSTRUCTIONS MODE 0A 00 T 07 PRINT POWER e Sample Compartment Wavelength Control for 10mm Test Tubes Knob WAVELENGTH or 10mm Square and Wavelength Readout Cuvettes Model 1100RS Spectrophotometer MODE INDICATOR Allows the operator to know the measurement mode currently in use Transmittance Absorbance MODE BUTTON Switches between Transmittance and Absorbance operating modes at any time of measurement OA 10096T BUTTON Adjusts digital readout to 100 T or 0 000A when blank reference solution is in sample compartment O T BUTTON When in transmittance T mode and sample compartment is empty a flap blocks the beam Pressing button adjusts readout to 00 0 T PRINT BUTTON ENT First press after selecting Concentration mode enters reading on digital readout Other presses cause output of reading to printer If using UNICO Application Software press this button to communicate with software If operating in Factor F Mode then press to enter factor This causes the Mode to enter the factor number and change to Concentration mode SAMPLE COMPARTMENT Insert 10mm test
13. ewjeq LN culdogzuld uewjeq usmjaq Ag 3exoos LE 36 33 jo WUIDIBDIJ PND ES Gb 36 34 6 Schematic Layout PART LIST OF UNICO 1100 SERIES SPECTROPHOTOMETERS I e 7 Mme O Fisse u Red O O i O 3 mome O O web 6 Reel 7 1 1 1 Power supply board 36 35 36 36 Fig 6 Schematic Diagram of UNICO 1100 Series
14. nal outputs O Volt to 1 Volt analog output and an RS 232C output Figure 1 3 1 and Figure 1 3 2 demonstrates the electronic systems of Model 1100 and Model 1100 5 respectively 36 6 22250 Aejdsiq Z 4 ainbly uabojey dwe usbojey Jo ejBuis Jo4uo2 9 E A uoDojeu Jo Ajddns AS AZIF AA AA oC 36 7 Aejdsiq L L uabojey dwe usbojey Jo ejBuis Jo4uo2 gt uoDojeu Jo Ajddns LEO AST AZL AA AA oC 36 8 1 4 SPECIFICATIONS Table 1 1 MODEL 1100 1100RS Wavelength Range 335 1000nm 335 1000nm Spectral Slit Width 20nm 10nm Wavelength Accuracy 2 2nm Wavelength Readability 2nm Stray Radiant Energy lt 0 5 T 340nm amp 400nm lt 0 5 T 340nm amp 400nm Transmittance O T to 125 T O T to 125 0 T Absorbancy OA to 2A OA to 2A Photometric Accuracy 2 0 T 1 0 T Photometric Noise Level 2 0 T 1 T Power Requirement 115 volt 60Hz or 230 volt 50Hz Size 408mm W x308mm D x180mm H Weight 5 5kg Specifications subject to change without notice 1 5 NOMENCLATURE Figure 1 4 Digital Display Mode Switch 0A 100 T Sample 0 1100RS Compartment Wavelength Readout Window Enter 10085 gt Wavelength Control 36 9 Figure 1 5 RS 232C
15. o big check the control single of halogen lamp O solving wiring problem of socket J5 O replace IC4 105 on the power supply board O replace power supply board 36 28 y HA ca Aiddns uamod Jo god 2 83 im 15 6 Y LSH 5 7 SY CY NEL Tue BO COO LLEF RISS 090069 EROS copo doo LA T Nat AB DA TAZL AZL 5V 121 12V L Gbl4 7 387205 609225029205 Ly 394205 P adwy indino A8F 39Y90S ZY oy yndino AZ 95205 ndino AG 38X930S QN9 yndul AZL yndul AZL AG ZUI4 Ulqd 39 o os El 9f SP er er 36 29 A A luni za 3 f ddng 1 JO UB1381 1109119 17175814 082121 36 30 gura Turd AMID 9 AG 2391008 TUIARGUIT ueasjaq On SUIqyveuld AZT USMJII 51 A ddn amp 195404 01 1195908 ev rd ess Rega Jo EFF x amp 4644646824446448 EYE 01 Qo GATA ma bibita 55 el Ely 674 v cIFSlSS 36 3 Jo uee TZ S814 Jus an RI Hr Tertczo E T PTSP SR d 20 jE I 36 32 jo jqueuebupuv god L OBA CODE LE 4155 u
16. ositior Close the sample compartment N Shutter working ju o Y Put the cell holder and cuvette into the sample compatrment Repair shutter assembly Press 100 after display 100 0 open the sample compartment cover if the display changde n Is regulate power supply normal Y Anplifier working N nmm 2 1 Solving wiring problem Refer Fig 5 6 to check amplifier Za 1 Solving wiring problem of J5 socket replace 4 741 507 1C5 40 51BE on the power supply board replace power supply Lio 36 25 TROUBLE SHOOTING 5 Problem Instrument on 100 0 display not stable T Enough warm up time N Groounding OK Y Power supply OK After changed wavelength stable time full lh 12V power supply work Regulate power supply working Warm up at least 20 min N Improuve grounding N Y Get rid of possible interference facters N fw After 5 min press 100 again N stable Y Replace PC power supply Y N Refer to Fig5 3 1 to check regulate supply of Electric eye working Replace the same ty
17. overheated components 4 2 OPERATING PROCEDURE CHECK Each time an instrument has a malfunction the operating procedure should be checked necessary such procedures should be repeated to see the patterns that the malfunction occurs The basic operating procedure is as follows Basic Operation SIMPLE OPERATING INSTRUCTIONS ARE PRINTED ON THE FRONTPANELOF YOURUNICO 1100 1100RS A Prepare the spectrophotometer 1 TURN ON THE SPECTROPHOTOMETERBY PRESSING THE POWER SWITCH IO ALLOW 15 MINUTES FORTHE INSTRUMENT TO WARM UP 2 SELECTEITHERTHE TRANSMITTANCE ORABSORBANCE OPERATING MODE BY PRESSING THE SELECTORBUTTON MODE UNTILTHE RED LIGHTFORT ORA 15 ON 3 SELECTTHE DESIRED WAVELENGTH BY TURNING THE WAVELENGTH CONTROLKNOB WAVELENGTH B Prepare Sample 4 MAKE A BLANK REFERENCE SOLUTION BY FILLINGA CLEAN CUVETTE ORTESTTUBE HALFFULLWITH DISTILLEDOR DE IONIZED WATEROROTHERSPECIFIED SOLVENT WIPE THE CUVETTEWITH TISSUE TOREMOVE THE FINGERPRINTSAND DROPLETSCF LIQUID 5 FIT THE BLANK CUVETTE INTO THE SQUARE CUVETTEADAPTERAND PLACE THE ADAPTERIN THE SAMPLE COMPARTMENT ALIGNING THE GUIDE MARK IF PRESENT WITH THE GUIDE MARK AT THE FRONTOF THE COMPARTMENT CLOSE THE LID 6 SET O 000A OR 100 T WITHTHE 100 CONTROLBUTTON NOTE THIS STEP FULFILS THE INSTRUCTIONON THE FRONTOF THE SPECTROPHOTOMETER SET FULLSCALE 7 REMOVE THE BLANK CUVETTEORTESTTUBE SETITASIDE IN THE CASE THATYOU MAY NEED TO ADJUSTTHE
18. pe electric eye S1227K Amplifier werving 36 26 N y Check amplifier TROUBLE SHOOTING 6 Problem measuring recelts incorrect not stable repeatability etc Is sample preparation adjust right qa n Is sample preparation Installed correctly y Are cuvettes used matched Y Is wavelength accurate Y Are T and 100 correct 7 Is photometric accuracy use right procedures to prepare samples make sure no bubble in sample test only after sample stability select right wavelength get rid of possible interference factors N Install the compartment correctly Use matched cuvetter Diference should be Within 0 596T z Calibrate wavelength Use right procedue to set 36 27 5 Drawing amp diagram Co 1 Check if the monochromator light passed onto the detector at right angle and right position area of the detector if not adjust it Amplifier output voltage static 20mV 30mV put black body to prevent the light from light way refer to Fig5 3 to adjust the potentiometer W to change the output voltage to 20mV 30mV measure the voltage between pin3 and pin4 Replace detector S 1227K Use NBS930D filter to check If the value of display to
19. tube and close lid or fit 10mm cuvette adapter and fit adapter with holder to left of cuvette into compartment 36 20 WAVELENGTH CONTROLKNOB WAVELENGTH Selects desired wavelength in nanometers nm WAVELENGTH READOUT WINDOW Displays desired wavelength 36 21 4 3 TROUBLE SHOOTING 1 Problem Turn on No display Display on N Check if LCD display and wiring OK TN Pilot light A Replace CPU board B Sloving wiring problem or replace socket A2 A3 Is 115V or 230v Power on P i Line power on Switch function Y Replace R13 69A switch PC power Function Y Replace PC power Check the 8V and 8V On the power supply board Refer to Fig 5 1 1 to check 8V and 8 power supply Is diode working fine How about wiring A Replace p3 green diode B Sloving wiring problem 36 22 TROUBLE SHOOTING 2 Problem Instrument is on Display 0 000 T or 1 P Check OK Halogen lamp on eck lamp Y l Replace lamp 2 Check output of lamp Blocking the light Power supply is it Between 1 6V to 6 2V V Y Refer Fig 5 3 to check Is cell holder
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