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1. There is no need for disassembly of the rollers other than in exceptional circumstances Special instructions are in an appendix to this manual and an outline is given below The rubber used for providing friction is now located on the Pump motor aia dite belt pump heads and can be simply pushed off the individual roller and exchanged as necessary This requires NO disassembly of the pump head system Service Manual Version 1 0 for SIXFORS Page 27 SIXFORS SERVICE MANUAL Exchange of the roller bars 1 Remove pump cover at the front 2 Remove rear panels 3 Loosen the two large screws holding the motor with a hexagon key do not remove and slide the drive belt over the top pulley to release it 4 Remove the screws holding the top of the supports for the rollers in the left middle and right positions so the drive belt can be slipped over the top pulley 5 Remove the 4 large screws 2 left 2 right with a hexagon key to allow the whole pump support frame to be lifted away Place on the bench for further dismantling 6 Remove the screws in the support frame 2 in each frame for holding the individual group of three pumps 7 Only one side need be removed to allow the pump heads to slide out individually 8 Remove the relevant pump head and replace as necessary 9 Reverse the procedure for re assembly Service Manual Version 1 0 for SIXFORS Page 28 SIXFORS SERVICE MANUAL 6 11 Removal of Top Cover amp M
2. A cable 9 Pin female 9 Pin female is available from INFORS Should your serial connection be to a 25 pin socket a standard 9 25 pin adaptor can be used to allow a direct connection It is possible to make your cable connections according to the table and schematic shown Note With 9 pin to 9 pin connecting plugs the data lines 2 and 3 must be crossed If a printer is to be connected this must be via a SERIAL connection or a serial to parallel converter must be used It needs no additional settings to be made Also when a terminal programme is used it is not necessary to issue a command for data to be transmitted Setup 1 Connect one end of the serial cable to the Sixfors Connect the other end to the 9 pin serial port of the PC Com1 or Com 2 3 Connect the earth lead at one end to a good earth point eg a casing screw 4 Refer to IRIS manual for operation from this point Service Manual Version 1 0 for SIXFORS Page 31
3. Feed Pump 21 5 7 IRIS Software Connections to Sixfors 22 6 Repair items 6 1 Testing of process parameters setup calibration actuators 23 6 2 Removal of covers basic sections of the Sixfors 24 6 3 Exchange of Pt 100 25 6 4 Removal and exchange of digital pcb 29 6 5 Exchange of heating element over temperature sensor 25 6 6 Exchange of drive motor 25 6 7 Exchange of rotameter inner cylinder 26 6 8 Exchange of operational amplifier module 26 6 9 Exchange of cooling valve in base section 27 6 10 Exchange of peristaltic pump components 27 6 11 Exchange of main pcb eprom micro processor 29 6 12 Exchange of screen amp keypad 30 T Installation of Optional Accessories Post delivery 7 1 Linking to IRIS software troubleshooting connection setup 31 Service Manual Version 1 0 for SIXFORS Page 2 WHERE TO LOOK BASIC INFORMATION INSTALLING A NEW SYSTEM CLEANING A UNIT ROUTINE EXCHANGE OF CONSUMABLES WHAT COULD BE WRONG HOW TO EXCHANGE KEY COMPONENTS HOW TO ADD ADDITIONAL ITEMS SIXFORS SERVICE MANUAL 8 Appendices SPECIFIC l l INFORMATION AS 8 1 PID drawings SixPOver doc INDIVIDUAL SHEETS 8 2 Electrical schematics Sixelec pdf Driverbus cdr 8 3 Pump head flow rate details Sxflowrate doc 8 4 Where to find the Serial No SerialCont doc 8 5 Contact details for Infors SerialCont doc 8 6 Sixfors Components What s Where SxWhtWhr doc 8 7 U DDC Instrumentation sixUDDC doc 8 8 Addendum to Sixfors U DDC
4. additional to it Max Influence sets a symmetrical limit in RPM in which control of pO2 can take place eg set point of 1000RPM with max influence of 200 would give control of pO2 by stirrer soeed from 1200 to 800 RPM only This is the only standard option Gas Mix and hand Mix only apply to systems with a special gas Mix unit Parameter Menu Fermenter F1 Parameter pH 1 Setpoint 2 Calibrate 3 PID Setup 4 Controller View 5 Calibrate all _ Select option Menu for Main Menu Enter or esc to Exit Fermenter gt F1 lt STATUS OK 00d00 00 Calibrate Fermenter F1 Parameter pH High Ref E 7 000 High Reading 2048 Low Ref E 4 1 Low Reading Slope 0 00379 Offset 11 98 Sensror Data Act read 0612 Act Value 0 00 Slp mV pH 56 3 Ref Temp 24 3 fee gt F1 lt STATUS OK 00d13 00 gt Pump Flow Settings Fermenter F1 Acid Pump Auto Cycle 60 Set 0 Base Pump Auto Cycle 60 Set 24 AF Pump Auto Cycle 60 Set 0 Feed Pump ON Cycle 60 Set 18 Flow Out Auto Value I min 0 Select Option Menu for Main Menu nter or Esc to exit Fermenter gt Fl lt Status OK 00d00 00 pO2 Control Def Fermenter F1 Flow Cont On RPM Cont On Max Infl 100 Gas Mix Off Hand Mix 0 Menu for Main Menu to change value Enter Esc to exit Service Manual Version 1 0 for SIXFORS Page 11 SIXFORS SERVICE MANUAL 3 Cleaning Before disconnecting the corrective reagent feed lines from
5. to work with stirrer Stirrer setpoint 0 Drive shaft not moving Can the drive shaft be turned by hand If NO unlikely No power to motor ls power being supplied to motor No power getting to motor from digital pcb Speed control malfunction Does speed match displayed value for speed visual estimation lf NO Is more than one motor affected If YES Motor bearings failure Culture viscosity is too high Service Manual Version 1 0 for SIXFORS Switch ON Switch pO2 Control by Stirrer OFF to test operation Check Stirrer set point gt O and deadband does not include zero rom Remove obstruction or replace bearings on drive shaft usually bottom bearing Replace digital pcb or motor Check connections Check connections to digital controller pcb Check connections to motor on digital pcb for power supply etc If PO2 control by stirrer is switched ON turn it OFF Replace motor Reduce speed Fit special EPROM Page 17 SIXFORS SERVICE MANUAL 5 3 Temperature System Faulty Pt 100 sensor Check connections Test calibration with eg ice and hot water No display or incorrect display of temperature Faulty amplifier module Replace amplifier module Check connections Processor problem Replace Pt 100 and re test Switch OFF and ON again Replace processor Control not active Ensure control is turned ON Control output not given Check indicator LED s is the heater Check connections light
6. 67Kg Electricity 230V 50Hz 10A noise free Water gt 1 5 bar Air 1 5 bar Service Manual Version 1 0 for SIXFORS Page 4 SIXFORS SERVICE MANUAL Overview of the SIXFORS Systems of Measurement amp Control TEMPERATURE PID Loop1 Temperature INPUT Op Amp Pt 100 OUTPUT Heater Cooling Valve ven STIRRER SPEED PID Loop2 Speed An IN OUTPUT Speed Ia Motor Tacho meter pH PID Loop3 pH INPUT OUTPUT Op Amp C 4 Acid Alkali Pump Pump DISSOLVED OXYGEN PO2 PID Loop4 pO2 INPUT OUTPUT1 OUTPUT2 Op Amp Motor Thermal Mass Flow Valve E Ee Temperature is controlled using an electrical heating block with in built cooling coil The range of control is approximately 5 C above the cooling temperature to 60 C A signal from a Pt 100 temperature sensor in the culture is used for feedback control by the micro processor to activate either the heater or the cooling valve The main controller display shows actual value setpoint PID control etc A setpoint is given to the control circuitry by the microprocessor The PID control values are factory set for this parameter The main display shows the actual value for speed The level of dissolved oxygen can be cascaded to
7. ON If NO Replace digital pcb No temperature control Processor Fault Check Fermenter Overview Check connections does this show heater cooling Replace processor or main pcb valve active If No Does the vessel get hot If NO No heating or inadequate heating Over temp cut out fault Check connections Replace cut out Faulty heating element Replace heater element Faulty digital pcb Check operation Neg factor too high control Check connections biased towards cooling Replace heater Check power output to heater Replace pcb Adjust No Water or inadequate flow Turn cooling water ON Check connections Set a low setpoint eg 5 C Does the aluminium block and vessel contents begin to cool f No cooling or inadequate NO cooling Check connections Replace cooling valve Faulty cooling valve Adjust eg if cooling water Incorrect Neg Factor temperature is too high Temperature drifts up or down PID settings incorrect Adjust esp P term default over time values available Service Manual Version 1 0 for SIXFORS Page 18 SIXFORS SERVICE MANUAL 5 4 pH System No display or incorrect display of pH No pH Control pH drifts up or down over time or acid amp alkali added almost continuously in turn Faulty pH electrode Temp compensation OFF Faulty amplifier module Processor problem Control not active Pumps not set to Auto No control in dead band No reagents Pumps not operating properly If pum
8. Profile Control 8 Pump Timings 0 pO2 Control def Fermenter gt Fl lt Status OK 00d00 00 Start Stop Fermenter F1 PH Control Off Temp Control Off Antifoam Off START FERMENTER Off pO2 Control Off RPM control Off Menu for Main Menu to change value Enter Esc to exit Fermenter gt Fl lt STATUSOK 0d 14 13 Setpoints Sl Ses gt F3 lt gt F4 lt gt F5 lt gt F6 lt RPM I oe I Temp I 37 0 I pH I 7 0 I pO2 I 80 I AF I Off I Men for Main Menu for Values Page 10 SIXFORS SERVICE MANUAL CALIBRATION Po eg eel ee ele 12 13 Calibration of parameters is possible for both active and closed down fermenters The RPM parameter cannot be calibrated as this is factory set Press Menu to get to Main menu 2 to select parameter menu eg 1 to select the fermenter 1 6 eg 2 to select the desired parameter 1 5 2 to select the Calibrate screen Enter the High Ref values using the numeric keys eg 600C and press Enter to confirm Press DOWN ARROW to move on The High Read is a value generated by the A D converter in the instrumentation press Enter to allow the reading shown in the Act Read display to replace the current value in the High Read position Use the DOWN ARROW key to pass over the value Repeat the process for the Low Value and Low Reading Press ENTER to conf
9. effectiveness is compromised the fermenter is functionally useless The port closure O rings are located in grooves in the closure and can be easily levered out for exchange Remember to lightly grease the new O ring Port fittings which have pipes going through them that fits over the length of the probe leaving only the tip exposed Service Manual Version 1 0 for SIXFORS Page 13 SIXFORS SERVICE MANUAL eaa pesgeniecwer inde Reagent Bottle Parts Disassembled These additional items are usually replaced by the user as necessary However as part of routine maintenance it makes sense to check any fittings in these perpiheral items eg dip tubes for corrosion and to replace clearly worn or damaged membranes and fittings Cap Dip tube Air filter Bottle Needle Membrane 4 4 Replacing drive bearings amp removal of drive shaft impelllors 1 Remove vessel top plate Undo nut holding the top of the drive shaft in place 3 Draw drive shaft past the top bearing support 4 Press on teflon bearing support to pop it out of its retaining metal frame Replace with a new teflon bearing 18217 Replace drive shaft and re assemble The bottom bearing support pops out of the centre of the ring sparger or triangular frame in the same way This is more likely to wear and needs regular exchange as a consumable item 22786 8 The impellors are exchanged moved by releasing the small grub screws holding them firmly
10. hexagon key needed holding the support block to the base unit 4 Push the block forwards slightly from the back and push the heater overtemp connector through 5 Remove the green water tubing by pulling it backwards off the connector firmly 6 The block is now free and can be upended so the heater and overtemp sensor can be unscrewed and removed and replaced as necessary Part no s 17648 22662 7 Onre assembly use a little heat conducting paste in the groove where the overtemp sensor is located 8 When replacing cooling water pipes check carefully for any leakage before replacing the covers 6 6 Replacement of the drive motor 17946 Heating element amp 1 Open front lower cover Ovetemp sensor 2 Remove digital pcb 3 Loosen small screws hexagon key needed to left and right of the motor Do not remove totally 4 Pull motor on supporting bracket forwards 5 Replacement is the reverse of this procedure Other electrical components can be exchanged 1 The 2 way magnetic cooling valves group of six in middle for cooling water distribution to support blocks Mains power inlet socket and filter Fuse 10A Mains ON light and green cover Operational hour counter Transformer for 24V supply Removal of drive motor eh eal eo ko Service Manual Version 1 0 for SIXFORS Page 25 SIXFORS SERVICE MANUAL 6 7 Exchanging the rotameter inner cylinder Calibration tables are included for each rotameter type in t
11. in the 13 5PG ports they have a finer thread than standard using adaptors if the electrodes are too long to fit without The other vessel fittings can be added such as sample pipe exit gas cooler and acid alkali inlets OPERATION 1 Air flow should be set to no more than 1 5 vessel volumes per minute eg for a 0 5L working volume vessel the max is 0 75NL min Pass the tubing from the reagent bottles through the appropriate peristaltic pump using the small connector in these lines to stop the tubing from being pulled through the pump Unclamp the connection between the bottle and the vessel Calibrate the 100 dissolved oxygen high value by passing air through the vessel at maximum flow rate when the stirrer speed is also at the maximum to be used At least 2 6 hours is needed before electrode is usable Complete the set up by setting appropriate setpoint values This should be sufficient for an installation check without autoclaving Check the electrode condition then prepare the electrodes for first use according to the instructions given in the relevant appendix i e pH remove white sealant over diaphragm completely pO2 unscrew base section remove diaphragm cartridge and half fill with electrolyte Replace A Service Manual Version 1 0 for SIXFORS Page 9 SIXFORS SERVICE MANUAL UDDC In
12. influence the stirrer speed Speed range is typically 20 1200 rpm upper and lower limits depending on the EPROM installed for a specific application No service adjustments can be made The pH measurement and control system consists of an electrode linked to a signal conditioning module which sends the measured value to the microprocessor Calibration is digital and similar to the procedure for a bench meter Range is typically pH 2 12 Integrated peristaltic pumps deliver a timed dose of either acid or alkali reagent into the fermenter vessel The total on time of each pump is recorded and can be used to measure how much reagent has been added Temperature compensation can be activated if required A gas valve for CO2 may replace the acid pump Measurement of dissolved oxygen is via an electrode linked to an integrated operational amplifier within the signal conditioning module A polarising voltage is also supplied by this unit Calibration is similar to a bench meter with zero and 100 values being set Control is by a PID loop acting on either the stirrer speed or by influencing an optional thermal mass flow control valve to adjust the air flow rate A gas mixing unit can be used for special applications such as animal cell culture Range 0 100 Service Manual Version 1 0 for SIXFORS Page 5 SIXFORS SERVICE MANUAL ANTIFOAM optional Control of foam is based on a conductivity PID Loo p5 Foam __ Probe above the cu
13. installation information UDDCinstS doc 8 9 Addendum PID Control Overview PiDover doc 8 10 Sixfors Outline diagnostic table SxFaults doc 8 11 Initial setup of electrodes ElecPrep doc 8 12 Service Agreement Checks for annual preventive maintenance SixAnSer doc Service Manual Version 1 0 for SIXFORS Page 3 SIXFORS SERVICE MANUAL 1 Dimensions Technical Data and Services Requirements Working volume No of vessels No parameters No reagent pumps Temperature Control Temperature Range Heating Stirrer Airflow Speed range s Data connection Profile storage pH range pO2 range Antifoam Feed Pump Dimensions Length x Depth x Height Weight Services Approx 0 3 to 0 5L Up to six round or flat bottom C rpm pH measure amp PID control pO measure amp PID control options antifoam or substrate cycle dose control Optional mass flow control and gas mix 3 per base unit Heater block temperature control incl cooling with a single walled vessel 5 C under coolant temp to 60 C 125W per station Electronic DC stepper motor with PWM control Bottom drive with magnetic coupling Stirrer bar or impellors amp baffles option 24 60NL Hr approx 20 1200 rpm RS232 Tx Rx and ground only Memory card type PCMCIA 2 12pH 0 100 Total cycle time and dose time 30 max Total cycle time and dose time 30 max Optional conductivity probe 9000 x 400 x 710 mm
14. on the front cover Remove the screen and keypad section complete with the front cover This can now be worked on separately There should now be free access to the main pcb Remove ribbon cables for connection to the base 24V power and the connection to the small instrument fan at the rear of the instrument housing Undo the 3 screws flat head at the rear of the main pcb Lift upwards and then back to remove the pcb 6 12 Replacing Screen and or Keypad 21557 1 IES Place glass screen face down on a cloth for protection Undo the 6 small nuts holding the LCD screen pcb on right Retain all spacers and ceramic washers Undo the earth lead at the bottom of the housing Disconnect small connector at the bottom of the keypad pcb which links two white leads from the LCD pcb to the small perpendicular pcb across the keypad area Lift LCD panel and remove replace as necessary Remove the small perpendicular pcb by undoing two small retaining nuts The keypad area can now be removed if required Re assembly le Fit the earth lead to the lower left hand side of the housing Put the 4 small spacers into place and replace the screen pcb Re fit the perpendicular pcb and re connect the white wires from the screen Fit and tighten the retaining nuts to hold the screen pcb into place Reconnect the earth leads Replacing main pcb Screen amp Keypad Interior LCD Screen amp Light Buzzer pcb 18335 removed Serv
15. the Mains switch peristaltic pumps they should be clamped off They can then be On the right hand side of the base unit emptied manually after re sterilization OFF On safety grounds the fermenter must be switched off and left for some minutes before removing the vessel s for cleaning of the base unit heating block can get very hot to the touch Cleaning the base unit and Master Control Examination of the vessel during cleaning panel During cleaning of the vessel an examination for 1 Use a soft damp NOT WET cloth anda any chips or cracks in the vessel glass can be made neutral detergent to wipe away any stains or and faulty vessel glasses MUST be replaced grime Any O ring seals which appear flattened or dry 2 Clean the unit down with electrical power off should be replaced and the new seals lightly and allow to dry completely before use greased with a mineral compound such as eg Edwards high vacuum grease 3 Any spillages of reagents especially acid or alkali must be wiped up immediately CARE Membranes which have been pierced should be NEEDED replaced Cleaning of a recently used system Please note sterilization of the vessel and peripherals MUST be done by the user prior to your Starting work on the equipment 1 The pH and dissolved oxygen electrodes should be removed and stored in suitable reagents according to the manufacturer s instructions which come with each electrode Copies are included in the ap
16. with zeroing gel and max speed air flow Use simulator if available Check slope of electrode for signs of ageing IS IT DRY Replace membrane or whole No display or incorrect electrode display of dissolved oxygen Faulty amplifier module Check connections Replace sensor or use simulator to test Replace amplifier module Processor problem Switch OFF and ON again Replace processor Control not active Switch control ON Actuator not switched to ON In Po2 Def screen select stirrer flow and or gas mix as appropriate Other settings must be made for flow and gas mix to work only control by stirrer is available as standard factory default is for flow control to be set ON so this must be changed No gas flow into vessel Check for bubbles in culture Fault with Actuator Check service supply a Drive Motor Check as in 5 2 No dissolved oxygen control Replace if necessary b Mass flow control valve Check connections Replace if necessary c Gas Mix unit Check connections Check services supply of gas Check solenoid valve operation listen for activation does valve get warm over time test for flow with air Replace valve if necessary Dissolved oxygen drifts up or PID settings incorrect Adjust esp P term default down over time or stirrer values available hunts Full 3 term control is often needed for good pO2 control Service Manual Version 1 0 for SIXFORS Page 20 SIXFORS SERVICE MANUAL 5 6 Antifoam Fe
17. Additional cables and equipment Reagent bottles are normally provided with the system These are set up separately and for test purposes can be filled with water See diagram for correct set up Service Manual Version 1 0 for SIXFORS Page 8 SIXFORS SERVICE MANUAL Bringing into use from a service viewpoint 1 Check all O ring seals for wear and replace as necessary Smear with a little high temp grease Undo the clamping ring using the screw at the front of the vessel The top plate can be lifted clear of the vessel glass which remains in place Check the large O ring seal 10962 in the grooved flange of the vessel glass is lightly greased not damaged and not wrinkled when in place Charge vessel with water to approximately the required working volume of the vessel Replace the top plate and tighten retaining clamp to finger tight Take care not to accidentally clamp the autoclaving cover for the Pt 100 in the clamp Equip any unused ports with a membrane collar and port closure if the port is to be used for additions post autoclaving If not a blanking port closure is sufficient Calibrate the pH electrode using the appropriate menu option in U DDC and buffers of eg pH 4 and 7 Follow the instructions to set a low and high reading The dissolved oxygen electrode low value calibration can be set using the zeroing gel provided CARE strongly alkaline solution Fit the pH and dissolved oxygen electrodes
18. If the Sixfors does not function at all on switching ON check the cable fuse laboratory earth trips and main fuse on the machine by the power indicator light A fuse is also present on the motor controller pcb printed circuit board Testing each parameter should follow a common pattern 1 Ensure relevant probe is in place look for signs of damage or eg no electrolyte or physical dents 2 Ensure correct services are switched ON eg water 3 Ensure the parameter has a realistic setpoint for the test eg to check the cooling valve the temperature setpoint may be set to 5 C 4 Check that control in Dead Band BD is switched to ON amp or the actual value for Deadband is near zero eg lt 0 5 5 Check calibration of parameter before starting fermenter 6 Check PID values for irregularities eg large value or no P value 7 Start fermenter and ensure the parameter is turned ON 8 If pumps or valves are involved make sure these are switched to Auto or have realistic manual set valves 9 Monitor the action of controllers via the Fermenter Overview screen and LED indicators in the third section 10 Use your senses for additional verification of actuators working eg if the heater works the vessel will get hot or you may be able to hear solenoid valves click on or off 11 Some parameters take a long time to get to fully operational condition eg dissolved oxygen electrode needs at least 2 hours with power supplied Don
19. SIXFORS SERVICE MANUAL Fermenters and Shakers Forover 30 years Cixfore reo a pn Service Manual A Display L Air Inlet V Magnetic Stirrer B Keypad M Operational Amplifier W Port Fittings C RS232 Port N Rotameter X Top Plate Clamp D Memory Card Slot O Water Pipe for Gas Cooler Y Support for drive shaft E Peristaltic Pumps P Water Valve for Gas Cooler Z Drive Shaft F Magnets in vessel Q Base unit Heater Block 1 Top Plate G Pt 100 Electrode Cable R Water Inlet 2 Overflow Pipe to Drain H pH Electrode Cable S Spring to retain vessel 3 Vessel Glass l Oxygen Electrode Cable T Vessel Support shelf 4 Impellors J Antifoam Probe Cable U S Steel Tray for Bottles 5 Sparger K Baffles not shown optional F QS Verk Handbuch Ser hb SixSer e doc Service Manual Version 1 0 for SIXFORS Page 1 SIXFORS SERVICE MANUAL SIXFORS SERVICE MANUAL Version 1 0 TABLE OF CONTENTS Page 1 Dimensions technical data and services requirements 4 2 Installation amp basic operation T 3 Cleaning 12 4 Exchange of consumable parts 4 1 Vessel seal 13 4 2 O rings on port fittings amp clamping ports 13 4 3 Membranes filters reagent bottle fittings 14 4 4 Replacing drive bearings amp removal of drive shaft amp impellors 14 4 5 Battery on memory card 15 5 Troubleshooting guides 5 1 Basic Operation 16 5 2 Drive System 17 5 3 Temperature System 18 5 4 pH System 19 5 5 Dissolved oxygen system 20 5 6 Antifoam amp
20. ached to the exit gas cooler if fitted If oxygen supplementation is used take care to pipe the exit air away safely to avoid explosion risk Service Manual Version 1 0 for SIXFORS Page 7 SIXFORS SERVICE MANUAL Vessel connections Temperature control The vessel needs only to be placed into the heater block by squeezing the smaller holder on the right hand side away and letting the vessel slide in The block now acts like a heater jacket and allows heat exchange with the vessel contents Inlet of water into the heater block for cooling is controlled by an automatic valve inside the bottom section of the base unit Connect the inlet and outlet pipes of the exit gas cooler using the small rapid coupling connectors Use the manual valve behind each vessel to turn the cooling water supply to the exit gas cooler ON The vessel top plate is equipped with a Pt 100 temperature probe This is in a 10mm clamped port and a 2 pin Lemo connector It is used for connecting to orange cable from the operational amplifier module Push to fit when the red spots on cable and connector are aligned Gas supply Fit a 0 22u filer to the top of the vessel sparger using braided tubing long enough to allow a clamp to be placed between the filter and the metal sparger pipe Connect the air line from the rotameter to the air sparger in the vessel by braided tubing pushing it onto the air filter If a gas mixing system is present connect th
21. ain pcb Modification Replacement of Master Units 1 Remove memory card 2 Undo 3 screws flat head at top and three at front of the instrument panel to release the top cover 3 Lift cover up and back gently to reveal the OP amp modules the top cover can be carefully rested on the back of the instrument housing 4 Remove all six Op amp modules 5 The main pcb is now exposed for simple work such as exchange of processor or EPROM Replacement of EPROM 22025 Sixfors A21 upgrading to eg add an analogue box or other additional facilities 2 The EPROM is located to the left of the large square microprocessor chip located in the top third of the pcb 3 Removal can be achieved using a chip extractor or by gentle leverage with a penknife blade or fine screwdriver CARE 4 Before placing the new EPROM in the empty socket check the pins are all properly aligned and if not gently press the side on the chip onto a hard surface to cause all the pins on that side to move inwards fractionally Replacement of the Microprocessor Location of EPROM amp Processor oriented Removal of Processor Service Manual Version 1 0 for SIXFORS Page 29 SIXFORS SERVICE MANUAL Replacing the main pcb 21696 1 2 3 Note positions of all ribbon cables and connectors Earth yourself for work on the main pcb Take out the 2 ribbon cables and the 3 pin cable joining the screen keypad unit to the main pcb Undo the 2 earth leads
22. e gas supply bottles to the appropriate inlets either to the rear of the base unit or on the separate gas mixing box Initial Setup Checklist Memory card in place Water line connected Drain line connected Air line connected Vessels connected to heating system Rotameter outlet connected to sparger Exit gas cooler inlet amp drain connected PT100 temperature sensor connected Air supply turned on Water supply turned on Mains power on Se ee le ee ee This is purely for testing the basic functions of the fermenter Connecting of probes etc is covered in a separate section Drive System The bottom drive is automatically connected when the vessel is placed in its holder Ensure the drive shaft if fitted is located correctly in the bottom bearing support the white teflon disk which is either in the centre of the ring sparger or in a separate triangular plate for round bottom vessels A special version of the EPROM can be specified if uncoupling is likely to occur during operation Connection to a computer Printer The SIXFORS has an RS232 serial port on the left side of the black support for the screen keypad If specified a cable should be provided which connects to this port and a 9 pin serial port at the rear of the computer Installation and use of IRIS software is the subject of another guide Printers must have a serial port or a parallel serial converter in order to link directly to the SIXFORS
23. e that no part of the SIXFORS sustains damage during unpacking Use the delivery documentation to ensure that all parts are present If there is a discrepancy between the list and the contentss and or there are any signs of transport damage please contact Infors at once If this advice is not followed promptly any resulting costs will be the responsibility of the purchaser All accessories and flexible connection pipes are usually packed in a cardboard box placed on top of the fermenter base unit The vessel is also packed in a separate cardboard box within the crate Services The final connection of the fermenter to the services will be made by INFORS if installation is requested by the customer This is aided greatly when all services outlets include the necessary pressure reduction system already installed by the customer Water Inlet Cold amp preferably soft lt 50ppm suspended solids water supplied at a pressure of gt 1 5 bar enters from the left side of the base unit of the fermenter Push on connector pipes are supplied separately in a bag Push one of these into place and fit braided tubing to the cold water tap Use jubilee clips to secure pipe to fitting at both ends Water Drain The drain outlet is on the left side of the fermenter It requires a clear fall to the drain sink without sharp bends or kinks Push fit onto metal barb connection Exit Air The exit air leaves via the disposable filter att
24. ed Pump Foam not sensed Foam always frequently detected Antifoam control does not work Feed Pump does not work see above for range of possible pump faults Faulty Antifoam electrode Check lead amp amplifier by shorting cables together does display show AF ON and or pump operates If YES If NO Processor fault Antifoam probe sheathing damaged unlikely Pump switched to ON Processor fault Control not active Pumps not set to Auto No reagent or inadequate Pump not operating properly If pump heads do not rotate Faulty pump motor Wrong tubing type Faulty Solenoid Is only one pump effected If YES Control output not given short out probe Check indicator LED s does the antifoam pump lights ON If NO Processor Fault Check Fermenter Overview does this show antifoam pump active If NO Pump not switched to ON same pump as antifoam No set value given Service Manual Version 1 0 for SIXFORS Check connections Replace antifoam probe Replace amplifier module Check connections Replace Replace probe Switch pump to Auto Check connections Replace Ensure control is turned ON Check and set to Auto if necessary pumps submenu Ensure bottles full right reagent amp connected Line open to vessel Check operation manually and prime pump Check connections Check drive belt Replace pump motor Check tubing type not too hard and spring tension Check connec
25. he Sixfors operating manual The scale on the Vogtlin rotameters is calibrated in Newton litres nr as standard and the type is given by the code after the manufacturer s name eg V100 80 To exchange the inner cylinder gauge glass which is the actual variable area tube 1 Undo the four screws flat head holding the transparent front cover 2 Undo the large screw at the top of the rotameter block using a hexagon key 3 The glass tube is now loose and can be replaced 4 Watch out that the front cover is the right way around when replacing as the screw holes are countersunk 6 8 Exchange of the operational amplifier pcb 17649 The purpose of the pcb is to digitise the analogue signals from the electrodes Exchange may be necessary if eg a sensor value cannot be calibrated properly even with a simulator Check the connections from the electrode cap and cable wiring before reaching this conclusion To exchange the pcb 1 Undo single screw flat head beneath individual op map module 2 Pull forwards and down gently to partly withdraw module 3 Whilst supporting the module remove the ribbon cable at the rear red wire to left hand end to release the module 4 Undo the 2 screws flat cheese head on top of the module casing Pull back and then lift to expose the op amp pcb and cable connector 5 When replacing the cover note the two lugs at the rear of the supporting part of the module which fit into slots in the back of t
26. he cover To adjust antifoam sensitivity Remove the Op amp board Remove the top cover Remove the ribbon cable to free the cover then replace At the front of the module where the cables are connected is a blue potentiometer marked 1 9 When there is no foam the diode above should be lit To reduce sensitivity turn the potentiometer away from the 9 until the light goes out 7 For the foam sensor in the vessel ensure the black wire is connected to the vessel top plate and the red wire is connected to the probe itself sig ee ee DO 4 e r f S A 5 Rotameter glass loosened Measurement module with opera amplifiers pcb Service Manual Version 1 0 for SIXFORS Page 26 SIXFORS SERVICE MANUAL 6 9 Exchange of an individual cooling valve 17801 1 Remove the rear panels Undo the two screws flat head holding the valve array to the right angled support bracket 3 Undo the electrical connector to the valves 4 Move valve for better access to the screws at the rear and remove the 4 small screws holding the valve body to the manifold Replace and reassemble 6 10 Exchange of peristaltic pump components The motor and drive belt for the peristaltic pumps can be accessed from the top rear of the base section The single stepper motor drives two rollers which operates any or all of gh om the 18 peristaltic pumps on demand This is normally switched La on permanently Above this are housed the peri
27. ice Manual Version 1 0 for SIXFORS Page 30 SIXFORS SERVICE MANUAL 7 1 Linking to IRIS Software Troubleshooting Connection amp Set up IRIS NT software provides data logging graphical display archiving and supervisory set point control SSPC for Infors fermenters The software must be installed on a PC running Microsoft Windows 9x or NT4 2000 The link to the Sixfors is made via an RS232 serial cable which connects directly to a port on the main pcb Control of the type of output and communications protocol is handled by special settings available in the Printer Comms menu option of the U DDC instrumentation Use of the Sixfors with a printer can also be controlled from here Details of installation of the IRIS software and operation with the Sixfors is given in the operating manual for IRIS NT This section deals with the very basic physical set up and simple troubleshooting for an installed system you may find already connected Serial cable connections Troubleshooting for an installed system See chart in Section 5 8 Connection to a PC or a printer 1 20 30 3 0 60 70 80 90 50 RS 232 On the side of the unit a male 9 pin connector is provided Description Computer Side Computer Side SDDC pins Pin Configuration 25 pins 9 pins mea ecelv erage Us pues Cota H Knaus No other connections are used by the SIXFORS For a functional data connection the cable must match this schematic
28. irm or Menu PUMP FLOW CONTROL This screen allows the built in reagent and feed pumps to be set to ON OFF or AUTO control Acid Base and Feed pumps can be ON OFF or AUTO eg when the acid and base pumps are under the control of the pH control system The feed pump can only be set to ON or OFF The cycle and set values are in seconds and allow different flow rates to be achieved by varying the time a pump is active over time The maximum duty cycle for any pump is 40 of the total cycle time eg cycle 60 set 24 secs This screen is reached via the Fermenter Options Menu Use the key to toggle On Off or Auto options DOWN ARROW moves on to the next position Enter the cycle time in seconds and use Enter to confirm the new value DOWN ARROW moves you on Repeat for the Set time The flow setting allows the thermal mass flow valve if fitted to be controlled with a manual value for gas flow rate in litres per minute This valve is usually under the automatic control of the pO2 control system A final Enter or Menu confirms the new values Esc aborts the process DEF PO2 This screen allows several options to be set for control of dissolved oxygen It is accessed from the Fermenter Menu If Flow Control is set to On using the key then oxygen control is via the optional thermal mass flow control valve If the RPM control is set to On then speed is used to control pO2 either in place of the flow valve or
29. lture surface detecting the build up of foam by contact with the INPUT OUTPUT probe tip The rest of the probe is insulated Op Amp to prevent false signals After a user selectable waiting time delay the integral peristaltic pump delivers a dose Antifoam of antifoam reagent shot Pump The system then resets and will only add more reagent if foam is still present This arrangement can double as a level control system to remove culture Probe GAS FLOW Gas Flow Rate Manual Unless specified otherwise control of gas flow Air OUT into the fermenter is usually set manually A ff separate pump or laboratory air supply is Rota i meter Connected to the fermenter supply pipework which has an integral pressure regulator valve The flow rate is adjusted using a rotameter calibrated in litres per minute hour The maximum air flow rate should be no more than 1 5 vessel volumes per minute A mass flow valve and or gas mixing system may also be used i I LT Lt a Air IN CHEMOSTAT OR CONTINUOUS OPERATION optional Medium IN This relies on a balance being set between addition of fresh medium into the vessel and INPUT OUTPUT removal of culture The correct rate matches the growth of the
30. microbes so that a constant ON concentration in the vessel is maintained 10 Secs peed OnE lf the growth of the organism IS limited by eg a source of sugar in the medium then the OFF Shot amp 50Secs Delay fermenter is being operated as a chemostat Keypad SnG 5 Ah Medium is added to the culture by a peristaltic pump which can be an integrated digital unit relying on shot and delay times or an external analogue pump with variable speed or 7 Analogue Or Analogue Medium OUT Harvest of culture can be achieved by using INPUT OUTPUT a level control system antifoam pump used Op Amp in reverse to withdraw culture or a simple eeel side arm acting as an overflow option Harvest l l Pump Even if the fermenter is operated as a chemostat for a short time large containers are needed for both fresh medium and harvest Antifoam reagent can be added to the fresh medium Probe at a low concentration eg 1 20 000 if required Service Manual Version 1 0 for SIXFORS Page 6 SIXFORS SERVICE MANUAL 2 Setup and Basic Operation Transport n view of the weight of the complete SIXFORS and packing materials it should never be carried by one person alone For moving the packed system over long distances a low trolley or palette raiser is recommended If the SIXFORS is to be transported by a fork lift truck it is vital it is secured in such a way that there is no cha
31. nce of it falling off or otherwise being damaged Location The SIXFORS fermenter is best mounted on a laboratory bench of suitable working height Access to air water and power services should be nearby along with a suitable drain sink A free space of 50cm should be left all around the fermenter to make servicing both easier and quicker The unit should be sited away from potential sources of electrical noise Services Connections Power 230V 10 A single phase supply for standard SIXFORS The power supply should be clean and constant if not it is recommend fitting of suitable filters and or a UPS to the mains power supply In addition to the plug fuse 2 fuses can be found behind a cover above the location of the connecting socket in the mains inlet and On Off switch Air The air inlet can be found on the left side of the fermenter Use only clean dry oil and dust free air with a pressure of gt 2 0 bar A push on Connector pipe is used with braided tubing Fit a jubilee clip at each end of the pipe to secure it Inlet air is supplied through the rotameter variable area flow meter to each vessel Ensure this is turned on using the black knob below the rotameter scale Calibration graphs are provided in the operating manual Packing and disposal The SIXFORS is shipped in a wooden crate All packing materials are environmentally friendly and can be re cycled Unpacking and Checking Please make sur
32. nd the display asks for memory card to be inserted If YES Processor Problem Memory card problem Remedy Plug in switch mains ON Check plug fuse 10A Check fuse in base unit 10A Check plug connections Test mains cable continuity Check connections at mains socket in base unit Check reset in plug socket Touch any key LCD screen should now light Check power supply to 24V feed from transformer Replace transformer if necessary Replace back light Check 24V feed to main pcb Replace screen panel Replace main pcb Memory card should be inserted N B Never insert or remove a memory card if the unit is switched on Switch On and OFF to clear fault If no change remove amp replace processor or exchange it for a new one Re initialise card remove battery briefly to clear mem Switch On and Off If no change replace keypad or processor try processor first For general random faults with operation pumps etc check earthing amp digital pcbs If setpoint calibration etc data is not being retained exchange memory card battery Service Manual Version 1 0 for SIXFORS Page 16 SIXFORS SERVICE MANUAL 5 2 Speed Control System Motor does not start Drive motor runs slowly or erratically pulses or hunts Drive uncouples at high speed Speed controller OFF s the speed control switched ON at Start Fermenter or Fermenter Options screens PO2 control switched on and set
33. ogue box Extras menu devices sub menu Check in Fermenter Properties dialogue box Click on Settings button in Peripherals dialogue box and check settings re fermenter manual Ensure the fermenter COM port is not shared check Control Panel settings in Windows Check in Peripherals dialogue box Extras menu devices sub menu Try different com port Ensure the fermenter COM port is not shared check Control Panel settings in Windows Click remote operation ON in the Edit Measured Parameter dialogue Set accept remote on the fermenter control panel Click backup value OFF in the Edit Measured Parameters dialogue Page 22 SIXFORS SERVICE MANUAL 6 Repair Items 6 1 Testing of Process Parameters setup calibration actuators etc The Sixfors can be thought of as a single container for measurement and control using at least 5 different operational sub systems i e temperature speed pH dissolved oxygen and foam feed This applies for up to six separate vessels see Section 2 for explanations of standard functions Part of fault diagnosis and repair requires that you have a knowledge of how each process parameter is controlled and setup for normal operation This requires familiarisation with the section of the operating manual dealing with the U DDC instrumentation A short guide is included in Chapter 2 for testing on installation but more detailed information is required for fault diagnosis
34. onto the drive shaft using a small hexagonal key oO Service Manual Version 1 0 for SIXFORS Page 14 SIXFORS SERVICE MANUAL 4 5 Battery on Memory Card The memory card AFC003 stores information about setpoints calibration PID setup profiles and other preferences A watch batttery preserves tha data on the card even when the SIXFORS is switched off After a period of 1 2 years the battery loses power and the user reports a loss of settings every time the unit is switched off A further reason for wishing to remove the battery may be that a PIN number password has been set and the user has forgotten it Removing the battery clears the memory The user will normally exchange the batttery themselves but may well ask for it to be done as part of preventive maintenance 1 Remove the card from the slot in the SIXFORS main pcb housing noting the orientation of the card 2 Asmall screw is found on the back edge of the card Remove this with the special tool provided with the card or using a watchamaker s screwdriver 3 Put your fingernail or eg a thin penknife blade into the small crack above the area containing the screw hole and pull backwards 4 A battery holder is revealed with the battery sitting in position Note the orientation of the battery and exchange for a new one TYPE XXXXXXX 5 Ensure the write protect tab on the back edge of the card is seet towards the middle of the card in the Write po
35. p heads do not rotate Faulty pump motor Wrong tubing type Faulty Solenoid Is only one pump effected If YES Control output not given Adjust set points Check indicator LED s do the acid and base pumps lights ON If NO Processor Fault Check Fermenter Overview does this show acid alkali pumps active If NO PID settings incorrect Incorrect strength of reagents Service Manual Version 1 0 for SIXFORS Check connections Test calibration with pH 4 and ph7 buffers Check slope of electrode for signs of ageing Replace or regenerate electrode Turn this to ON in normal use Check connections Replace sensor or use simulator to test Replace amplifier module Switch OFF and ON again Replace processor Ensure control is turned ON Check and set to Auto if necessary pumps submenu Ensure this is OFF or small value Ensure bottles full line open amp connected to vessel Check operation manually and prime pumps Check connections Check drive belt Replace pump motor Check tubing type not too hard and spring tension Check connections to solenoid Replace solenoid Check connections Replace digital pcb in base section also for pump mal functions Check connections Replace processor or pcb Adjust esp P term default values available Check reagents 0 1 2 0M Page 19 SIXFORS SERVICE MANUAL 5 5 Dissolved oxygen system Faulty pO2 electrode Check connections Test calibration
36. pendices of this operating manual 2 Periodic cleaning and regeneration of the electrodes are also covered by these instructions 3 The vessel should be rinsed several times in distilled water to remove any loose culture residues 4 Growth of culture on the vessel walls may require disassembly and light brushing to completely clean the glass Service Manual Version 1 0 for SIXFORS Page 12 SIXFORS SERVICE MANUAL 4 Exchange of Consumable Parts During a routine service inspection a number of consumable parts may have to be exchanged 4 1 Vessel seal The vessel seal is critical for maintaining an uncontaminated environment for the culture 1 Remove the clamping ring from the vessel to release the top plate 2 Carefully lift the top plate upwards so the pipes etc clear the lip of the glass section and place the top plate gently on its side away from the glass section The glass section will continue to be supported by the one 4 The new seal is very lightly greased with silicone grease and placed in the groove It must fit snugly without any wrinkles If a good fit cannot be achieved discard the seal and use a new one 5 Re assemble the top plate onto the vessel glass and replace the clamp using even pressure the clamping screw finger tight only 4 2 O rings on port fittings amp Clamping Ports The O rings seal the port closures against the top plate so no microbes can get in or out If their
37. sier to carry a spare Pt 100 sensor known to be calibrated properly ice water and boiling water provide 2 certain reference points at normal altitudes and atmospheric pressures If you do not have access to a simulator useful tests can still be performed on site LED RELATES TO THE INDIC ACTION OF ATOR Indicator LEDS in the Base section of the Sixfors Page 23 SIXFORS SERVICE MANUAL 6 2 Removal of Covers Main Sections of the Sixfors The base unit of all Sixfors contains the following Bottom Section rear 1 3 panels held by 4 screws each flat head 2 For left amp right panels lift reagent bottle platform out of the way by lifting back and pulling backwards at an angle of approx 30 to release front lugs 3 Remove panel s 4 The middle panel is popped out by moving it slightly left and pulling out and rightwards to get it free of the instrument support struts This panel is replaced first You have access to Left Side e Water outlet Middle e Solenoid valves x6 for cooling water Bottom Section Left rear e Pcb cooling fans for each position Bottom Section lower front Undo the 10 screws along the length of the front bottom panel Bottom Section and lift forward and downwards gently to gain access to Right Rear Je 0 Ris T eb oe D i AT hse Bottom Section upper front 0 V 4 EPR ayy Rotameters for air flow control Manual control valves for exit gas cooler
38. sition before replacing it in the SIXFORS The card will be initialised by the SIXFORS on first switching the fermenter on and all setpoint values set to zero with defaults for all other data If the customer has downloaded the current configuration to a PC the data can be restored to the card using Option 6 in the Main Menu Copy Memory Card Memory Card Screwdriver Screw Battery Write protect Holder tab Service Manual Version 1 0 for SIXFORS Page 15 SIXFORS SERVICE MANUAL 5 Trouble shooting the Sixfors 5 1 Basic Operation Sixfors does not work at all No green mains power light Mains power but no display LCD Screen halts at P O S T Screen Power On Self Test Screen shows random characters and or odd behaviour of pumps etc System hangs or does not respond to inputs Keys fail to work Processor problem or fault with keypad Power lead not plugged in or Mains power not turned ON Fuses blown Break in power connections Unit switched ON but not touched for longer than 30 minutes No 24V supply Can the red LED s indicating 24V supply be seen in the LED display in the base section of the Sixfors for each unit If NO No Back light Can figures be seen dimly in the LCD display If YES No 24V supply to pcb Main pcb or back light faulty If 24V supply to main pcb OK can peristaltic pumps be operated manually If YES If NO No Memory card Do all tests show pass a
39. staltic pumps Pe the solenoid magnets which pull the pump heads onto the E Ah MA iP Uke 2 HE Oe ia roller CAA FF RAD llers E aN i aA a Access to the pumps amp rollers om a os gt SCA 1 Undo all pump clamping bars and lift out of the way Undo a single screw at the back of the top section of the base unit and the 4 screws in front all flat head 3 Lever the top panel gently out of the way lift towards the front then slide along to the left when free of the pump heads Changing a solenoid 17955 1 Remove the rear panels 2 Undo the two screws flat head at the top of each solenoid 3 Lift the solenoid forward to clear the claw of the lifting bar for the pump head Pull down to free the solenoid Cut or de solder the wires to the solenoid The block of three pumps for each vessel position can be disconnected using the white connector block below each pump grouping ou ela Changing the pump drive belt 17814 1 Loosen the two large screws holding the motor using a hexagon key 2 Lift the motor up slightly and support it 3 The drive belt is now loose enough to remove from the upper pulley Removing the motor for the peristaltic pumps 17946 Take out the two screws holding the motor Remove the drive belt Unclip the connector for the motor wiring Cut the earth wire to the motor The motor is now free for removal Check the tension of the drive belt on replacing Oak whd
40. strumentation basic points e The UDDC2 instrumentation is based on a powerful industrial process controller which uses a memory card type PCMCIA to store data about Set points profiles etc e The keypad is splashproof and has several sections each with keys which perform specific tasks Numeric keys 1 9 plus Data Entry numbers 1 6 Select fermenter Toggles options Enter Confirmation Esc Abort last action UP ARROW Moves upwards Moves downwards Go to Main Menu DOWN ARROW Menu e The screen is a high contrast LCD unit displaying 40 characters by 16 lines Different areas of the screen provide different sorts of information Top Line Fermenter data Second line Current option Lines 4 13 Fermenter values Lines 15 16 HELP information e A square flashing cursor appears in a number of screens against something which can be changed STARTING OR STOPPING A FERMENTER 1 To start a fermenter from the Main Menu select Option 1 fermenter Menu 2 The screen changes to allow you to select which fermenter 1 6 to start using the numeric keypad 3 The Fermenter Menu is then displayed Select Option1 Start Stop 4 Inthe start Stop screen use the DOWN ARROW key to move through the options deciding which parameters to keep On and which to switch Off using the key 5 Finally use the key to switch Start Fermenter to On and press either Enter or Menu to confirm 6 Men
41. t take the first value as correct especially soon after autoclaving 12 Look at measured values and the raw A D converter readings on the calibration page do they roughly match eg pH7 approx 512 an A D reading of 100 or 1000 would suggest a fault see addendum for more info 13 Unexpected values or odd behaviour from measurement probes can sometimes indicate an earth problem which may not show up on a conventional electrical test eg the dissolved oxygen electrode is very sensitive to a poor earth connection leading to the value drifting high Default values for calibration and settings are provided within the EPROM for re initialised cards and new installations see separate addendum UDDC installation information sheet The next stage in fault investigation usually requires you gain access to the relevant actuator for more direct tests CHECK COMPONENTS BY SWAPPING WITH THOSE IN ANOTHER UNIT OF THE SAME TYPE IF POSSIBLE Service Manual Version 1 0 for SIXFORS pH and Dissolved Oxygen Simulator Calibration is best achieved using a certified simulator for pH and dissolved oxygen as this tests both the cable and amplifier a suitable unit is made by the Valley Instrument Co Inc of Exton PA USA If calibration is successful using the simulator incorrect readings can then almost certainly be attributed to a faulty probe or earthing problem A simulator can be used for temperature also but it is often cheaper and ea
42. tions to solenoid Replace solenoid Check connections Replace digital pcb in the lower front of the base also for pump malfunctions Check connections Replace processor or pcb Switch pump ON Give cycle amp set times secs Page 21 SIXFORS SERVICE MANUAL 5 7 IRIS Software Connections to Sixfors assumes prior correct installation by Infors No Data logging or control Error opening com port Message box appears Logging but no Control No change in logged value Cable not connected Cable connected to wrong COM port Cable connected to wrong fermenter Cable not earthed at one end Fermenter not switched to remote operation COM port settings incorrect Fermenter mis assigned Device model incorrect eg set for ISF not Labfors Wrong protocol for comms Eg baud rate or parity mis set Conflict with other devices on COM port eg a mouse COM port settings incorrect COM port not existing on this computer Conflict with other devices on COM port eg a mouse Parameter not set for this Fermenter settings are not correct Backup value may be ON look in Text view table Service Manual Version 1 0 for SIXFORS Connect firmly at both ends Check which COM port a fermenter is assigned to and swop if needed Swop cable if necessary Ensure earth strap connected at the computer end to a case screw Select this option at the Fermenter instrumentation Check in Peripherals dial
43. u takes you back to Main Menu and pressing Esc will let you see the Values screen 7 To stop an active fermenter select Option 1 Start Stop from the Fermenter Options menu and use the key to toggle Stop fermenter to On amp press Enter or Menu to confirm the shutdown CHANGING A SETPOINT There are two ways to change a setpoint 1 From the Values Screen simply press Enter after first selecting the fermenter 1 6 and then the required parameter with the cursor using the UP DOWN keys for navigation 2 Alternatively press Menu to show the Main Menu and press 1 to select the Fermenter Menu 3 Press 2 for the Parameter Menu The Parameter Selection sub menu will allow you to choose the parameter you want to change eg 1 RPM 4 Anew setpoint is entered using the numeric keys and confirmed with Enter 5 DOWN ARROW moves to the Alarm Lim field where a symmetrical alarm limit value can be entered outside of which an alarm will be generated 6 Press Enter to confirm the value and Enter again to return to the parameter menu Service Manual Version 1 0 for SIXFORS f f Se edit setp 1 Fermenter Menue 3 Printer Comms 5 Password 1 Start Stop 3 Fermenter 5 Edit Profile 7 Ferm Overview 9 Alarms A Main Menue oN 2 Parameter Menue 4 Hardware Options 6 Copy Memory Card d Fermenter Menu Fermenter F 1 2 Parameter Menu 4 Pump Flow Settings 6
44. water inlet Three peristaltic pumps per station Peristaltic pump heads Rollers x2 Solenoid valves to pull pump heads onto roller O LE Cl a ee 6 3 Main pcb Housing Screen and Keypad From the front side you can gain access to Memory card slot on left RS232 serial port for printer computer connection on back Small instrument fan Screen amp keypad Backlight amp buzzer pcb a Top Section The mains socket main fuse the hour counter ID plate are at the right rear of the base unit Service Manual Version 1 0 for SIXFORS Page 24 SIXFORS SERVICE MANUAL 6 3 Exchange of Pt 100 1 Locate Pt 100 on vessel top plate 10mm port 6 4 Exchange of the digital pcb in the lower front base 1 Undo single screw flat head at front of pcb on heat sink 2 Pull pcb forwards part way 3 Remove 4 pin connector to the motor and the 2 pin connector to the small fan behind the pcb 6 5 Exchange of Heating element amp over temperature sensor Supplied together The indication of a need for exchange will be 1 No heating maybe a dead short on the element also check using a multi meter 2 The controller output indicates heating is called for 3 There is power entering and leaving the connections on the digital pcb to the heater To exchange the heating element amp overtemp sensor 1 Remove rear covers 2 Disconnect heater over temperature sensor cable white connector 3 Undo the 2 x large screws

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