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Figure 7-1

1. DILUTER LINEARITY AND PHOTOMETER LINEARITY AND PHOTOMETER HALOGEN FLUID DYNAMIGS saatan kundi diana hee ubi cw PHENOMENON OF DILUTION OF SOLUTIONS WASHING SOLUTION SUPPLY esee SENSOR farran Ere He eet eo CONTAMINATION THE TEST WITH COLORIMETRIC METHOD TEST WITH ENZYMATIC HYDRAULIC CIRCUIT RESIDUUM VERIFICATION SAMPLE VISCOSITY 340 700 CARRY OVER IN SERUM avtar eit SAMPLE SECTION III ELECTRONICS TITLE TOP VIEW THE BT 3000 PLUS itte ANALYZER DESCRIPTION OF ANALYZER COMPUTER READING STATION INDEX Page 3 of 12 SECT II II II II II II II II II II II II II II II II II II II II II II SECT BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS PARA TITLE SECT 3 3 3 POWER SUPPLY MODULE time tete ane o at se ende 3 3 4 REAGENT SYSTEM eee 3 3 5 SAMPLEING MODULE niai 3 3 6 SAMPLING ARM MODUL
2. Baude Rate 9600 1 Panty ies None Hand shake Hardware Important Notice These two programs are in the installation disk under Utility folder SECTIONIV Page 18 of 18 SECTION V REPAIR OR REPLACEMENT OF VARIOUS PARTS DVD CD ROM LCD PLAYER e DISPLAY 000000 00000 90 pP FLOPPY 27212 DISPLAY ON OFF SWITCH STATION REAGENT PLATE REAGENT CHAMBER WASHING FUNNEL REAGENT BOTTLES SERUM CHAMBER VACUTAINER SENSOR mm SENSOR c d SERUM CONTROLLER 15 04422 01 BT2000 PLUS FRONT VIEW Figure 5 1 5 1 Access to Various Modules Introduction The BT1000 amp BT2000 PLUS chemistry analyzers require a series of mechanical adjustments during the production and assembly process to assure optimal performance The same mechanical adjustment procedures that were followed in the production are outlined here for field maintenance and repair The analyzer is based on a modular construction Each module must be correctly inserted into its proper location Any positioning adjustments are facilitated by the large tolerances on the mounting holes SECTION V Page 1 of 16 It is important to check that the sampling arm movement precisely coincides with all the operating positions A fine adjustment of positions can be mad
3. STEPPING MOTOR BEARING ASSY a REAGENT i PELTIER n ASSY oc PULLEY lo VT SS REAGENT CHAMBER Figure 7 7 SECTION VII Page 4 of 6 Peltier Module N o REFRIGERATOR MODULE BOTTOM Figure 7 8 TIMING PULLEY PELTIER TIMING BELT TEMP SENSOR HALL SENSOR REFRIGERATOR MODULE TOP Figure 7 9 SECTION VII Page 5 of 6 VARIOUS DEVICES CONTROLLER MODULE Sun Reagent Controller Module Figure 7 10 REAGENT CONTROLLER MODULE Reagent Devices Figure 7 12 SECTION VII REAGENT CONTROLLER MODULE Reagent Controller Module Figure 7 11 N REAGENT MOTOR CROSSBLOWER FAN Reagent Devices Figure 7 13 Page 6 of 6 SECTION VIII SAMPLING STATION 8 1 General Information The sampling station is one of the various modules of the analyzer It consists of the following devices Serum Plate Washing Funnel Sampling Arm Barcode and the Diluter Pump Though the diluter pump is the integral part of the sampling system but it is located on the Reading Station module Refer to Figures 8 3 amp 8 5 for location of various devices of the sampl
4. COMPUTER MODULE TROUBLE SHOOTING SYMPTOMS CAUSES AND CORRECTIVE ACTIONS After loading Windows the loading of the process program starts and is visible on the progression bar The progression bar inactive followed by an error message Impossible to Reset Analyzer All electro mechanical devices remain motionless 1 No communication between the computer and the process electronics Check that the flat cable across IBM and the Main Logic Board on the rear of the reading station is properly connected 2 Defective RS 232 serial communication port of the computer or the micro controller Check and replace if necessary 3 Defective Main Logic Board Verify and replace if necessary No communication between the analyzer and the host computer Defective relevant serial ports or interrupted connecting cable Verify and replace serial port Check that the interface cable is correctly connected NOTE If the interface with the host computer is done for the first time then there may be a software problem To avoid software problems use specific software protocols for verification of communications 13 2 2 Trouble Shooting the Bootstrap BOOTSTRAP COMPUTER MODULE TROUBLE SHOOTING SYMPTOMS CAUSES AND CORRECTIVE ACTIONS After turning on the computer the bootstrap process interrupts and displays an error message NT
5. i T FLOPPY ON OFF SWITCH LCD Display i i BT2000 PLUS FRONT PANEL Figure 9 3 9 1 ELECTRONICS OF COMPUTER MODULE See preceding figures 9 1 1 COMPUTER MODULE The LCD Display module Figure 9 3 is on the front left of the analyzer and accessible for service from the rear of the computer module The peripherals DVD CD ROM player and the floppy disk drive Figure 9 3 are located on the right of the LCD Display An on off switch green button Figure 9 3 for the analyzer is located on the right just below the floppy disk drive All of the connections Figure 9 1 to the external peripheral devices are located on the rear of the LCD Display in the computer module as follows 1 Parallel Port for the Printer 1 Serial Port for Host Computer 1 Serial Port for UPS 3 USB Ports Q for optional peripheral equipment 2 PS 2 ports for Keyboard amp Mouse 1 Serial Port COM3 for optional peripheral equipment 1 5 Connector for external monitor The ASUS main board with a 1 GHZ Intel Pentium III processor 128 Mb RAM and the peripheral boards are all affixed inside the Computer Module 7 The Hard Disk 8 is secured under the computer module The computer Power Supply 9 is located above the Computer Module over the LCD display panel SECTION IX Page 2 of 7 The analyzer Power Supply 40 Floppy Disk Drive amp the DVD CD ROM Player behind the power inlet
6. Ix INDEX Page 7 of 12 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS PARA TITLE SECT Fig 9 4 COMPUTER MODULE ee ten IX Fig 9 2 COMPUTER MODULE OPENED arte IX Fig 9 3 2000 PLUS FRONT PANEL mmm t petri edd IX Fig 9 4 MOTHERBOARD CONNECTORS IX SECTION X POWER SUPPLY PARA TITLE SECT 10 1 POWER SUPPLY ana o steel ei edut D 10 2 5 nare docct X SECTION XI VACUUM PUMP MODULE PARA TITLE SECT 11 1 THE WASTE PUMP amp THE WASTE XI 11 2 WARNINGS uimassa XI 11 3 TECHNICAL SPEGIFIGA TIONS du Jenssen sath nie saan kaa XI 11 4 SPARE PARTS FOR REPAIR 8 XI Fig 424 WASTE PROBE goat cie ft dise XI Fig 11 2 FEMALE FISCHER CONNECTOR XI Fig 11 3 VACUUM PUMP MODULE intentare ind XI Fig 11 4 VACUUMIPUMP Assam XI Fig 11 3 H20 TUBE WITH FILTER suvea tod ten reed XI INDEX Page 8 of 12 PARA 12 1 12 1 1 12 1 2 12 1 3 12 2 12 2 1 12 3 12 3 1 12 3 2 12 4 12 4 1 12 4 2 12 5 12 6 PARA 13 1 13 1 1 13 1 2 13 1 3 13 1 4 13 1 5 13 1 6 13 2 13 2 1 13 2 2 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS SECTION XII
7. COMPUTER MODULE TROUBLE SHOOTING TROUBLE SHOOTING THE 2 XIII INDEX Page 9 of 12 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS SECTION XIV SPARE PARTS FOR REPAIR AND MAINTENANCE PARA TITLE SECT 14 1 READING STATION MODULE SPARE XIV 44 2 REAGENT REFRIGERATION SYSTEM SPARE PARTS NO XIV I REFRIGERATION IN BT 1000 14 3 SERUM MODULE SPARE XIV 14 4 SAMPLER ARM SPARE XIV 14 5 VACUUM PUMP MODULE SPARE XIV 14 6 CONSUMABLES 2 ii 14 7 ELECTRONICS MECHANICAL 2 14 8 ORDERING INDEX Page 10 of 12 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS lt lt BT 1000 amp BT2000 PLUS gt gt SCHEMATICS AND DIAGRAMS SCHEMATIC SE 04209 02 SE 04209 02 SE 04211 02 SE 04211 02 SE 04270 01 SE 04270 01 SE 04276 01 SE 04276 01 SE 04278 02 SE 04278 02 SE 04280 01 SE 04280 01 SE 04282 01 SE 04282 01 SE 04313 02 SE 04313 02 SE 04422 01 SE 04422 01 SE 04431 02 SE 04431 02 SE 04649 01 SE 04649 01 SE 04651 01 SE 04651 01 SE 04661 01 SE 04661 01 SE 04680 02 SE 04680 02 SE 04765 01 SE 04765 01 SE 04767 01 SE 04767 0
8. 0 20 2 9 Reagent Refrigeration BT 2000 PLUS The purpose of reagent refrigeration is for optimal conservation and reagent performance The temperature inside the reagent chamber is variable based on the external ambience and the time inside the refrigerated chamber A reagent bottle inside the chamber gradually reaches the minimum temperature within 2 hours The temperature range may go from 10 C to 22 C with outside temperature of 20 C and 32 C respectively Note The BT 1000 is not eguipped with the refrigeration module SECTION II Page 4 of 12 2 10 Diluter Linearity and Accuracy A unique diluter system is used for the processing of chemistries and ISE testing Technical Specifications for the Uniflows HSO53AZ20 dilutor Maximum volume Resolution Accuracy F S Accuracy at Linearity from 10 to 500 1 Reproducibility at 3ul Reproducibility from 5 to 500yl Average Life Maintenance 2 11 Spectral Response Bandwidth Linearity O D Resolution Drift 530ul 0 09 ul per step 0 09 3 gt 1 1 Better than 0 7 gt 3 million cycles Every one million cycles Photometer Linearity and Accuracy From 340nm to 700nm 5nm max 1 from 0 to 2 000 2 5 from 2 000 to 2 400 0 0005 Abs 0 0005 Abs per hour after warm up Note The specification regarding drift applies exclusively to the photometer excluding the drift related to the
9. CUVETTE SYSTEM Figure 6 4 SECTION VI Page 4 of 11 OUTPUT CONNECTOR 6 4 LINEAR ACTUATOR PERISTALTIC PUMP RESERVOIR LIQUID LEVEL DILUTER PUMP SENSOR WASTE OUTLET PINCH VALVE CONNECTOR FOR ee JON SAMPLING ARM WASHER PINCH VALVE TO DILUTER WASHING PISTON WASHING Washer Diluter Reading Station Figure 6 5 Diluter and Washer Module The upper part of the reading station consists of a PVC panel which accommodates the following devices 1 X A 530yul diluter pump providing the analyzer with high precision reproducibility and reliability The stepper motor and the photo sensor cable is connected to the Master Power Board 15 04209 02 on the rear of the PVC panel A pinch valve peristaltic pump and a connector for sampling arm described in detail in Section VIII entitled Sampling Station are located on the upper left of the washing station The cuvette washer module is positioned in a similar manner to the previous version used on BT 2000 3000 The washer module utilizes a bi directional linear actuator based stepper drive mechanism to provide the washing piston carriage rapid linear movement in up down directions A photo switch is provided for reset and standby position The actuator stepper motor and the photo switch cable connects to the Master Power Board 15 04209 02 located to the rear of the PVC panel These are all managed by the microcontroller uC764 Most of th
10. 5 4 2 REPLACEMENT OF THE HALOGEN V 5 4 3 REPLACEMENT OF HYDRAULIC V INDEX Page 4 of 12 PARA 5 4 4 5 4 5 5 4 6 5 4 7 5 5 5 5 1 Fig 5 1 Fig 5 2 Fig 5 3 Fig 5 4 Fig 5 5 Fig 5 6 Fig 5 7 Fig 5 8 Fig 5 9 Fig 5 10 Fig 5 11 Fig 5 12 Fig 5 13 PARA 6 1 6 2 6 2 1 6 2 2 6 3 6 4 1 6 4 2 6 5 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS TITLE SECT REPLACEMENT OR MAINTENANCE OF DILUTER REPLACEMENT OF THE WASHING REPLACEMENT OF THE READING PHOTOMETIERREPEAGCEMENT 2 1n Ie ro tete V INTRODUCTION TO THE COMPUTER MODULE V REPAIR AND REPLACEMENT OF COMPUTER MODULE V NET eed DAN RUN ARES SAQUE v BT2000 PLUS FRONT VIEW V SAMPLING ARM FRONT VIEW cene V SAMPLING ARM REAR VIEW s heat went teste e V READING STATION MODULE ee V REPLACING THE HALOGEN V LAMP ACCESS COVER V WNAE V SHUTTER FRONT PANEL ere tente V WASHER MODULES cxx were ars pm an V EEBEAGING THE CUVE TIE S ee c eee V PHOTOMETER MODUE es V COMPUTER MODULE ELECTRONICS 3 V sepe pur
11. V SECTION VI READING STATION SYSTEM TITLE SECT ENE EI A VI PHOTOMETER MODULE VI SOURCE ann VI REACTION CUVETTE OPERATING PRINCIPLE VI READING CUVETTE SYSTEM coe miss s VI DILUTER AND WASHER MODULE VI PILER MODULE S riesen cene VI WASHER MODULE SYSTEM VI ELECTRONICS FOR THE READING STATION SYSTEM VI INDEX Page 5 of 12 PARA 6 6 6 7 6 8 6 9 6 9 1 6 10 Fig 6 1 Fig 6 2 Fig 6 3 Fig 6 4 Fig 6 5 Fig 6 6 Fig 6 7 Fig 6 8 PARA 7 1 7 2 Fig 7 1 Fig 7 2 Fig 7 3 Fig 7 4 Fig 7 5 Fig 7 6 Fig 7 7 Fig 7 8 Fig 7 9 Fig 7 10 Fig 7 11 Fig 7 12 Fig 7 13 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS TITLE MASTER LOGIC BOARD 15 04211 02 MASTER POWER BOARD 15 04209 02 INTERFACE BOARD 15 04270 02 THE 10 CHANNEL TECHNICAL SPECIFICATIONS OF THE PHOTOMETER EGG PROCEDURE Loser teaser esta tte eee READING STATION MODULE seem PHOTOMETER MODULE tete diced CUVETTE PLATE rn FED Erden E o anes OUVETTE SYSTEMS iue E t sede WASHER DILUTER vike terne ee RAINER RU KITA KANTOLAN DIETER MODULE Luanda iOS READING STATION REAR PHOTODIODE ARRAY ten tern tinh Ret SECTION V
12. Insert a new halogen lamp fully into the socket and firmly tighten the screws to secure the lamp It is recommended to slightly press both of the lamps retaining spring clips before fitting the lamp Slide the lamp assembly onto the light cone and orient the lamp socket in the vertical position as shown in the Figure 5 5 Shutter Front Panel LAMP ACCESS COVER REMOVAL LAMP REMOVAL Figure 5 6 Figure 5 7 1 SHUTTER FRONT PANEL Figure 5 8 5 4 3 Replacement of Hydraulic Tubes There are two tubing and accessory kits for maintenance A Six Month Kit for special tubes exposed to wear and tear including pump cartridges etc and an annual maintenance kit for general tubing for interconnections and liquid transfers When installing any tubing kit it is important that the hydraulic circuit is empty To avoid any undesired liquid spills disconnect the external water input before applying any maintenance kit Turn on the instrument and perform an EMPTY CYCLE to evacuate all liquid in the hydraulic circuit When changing tubes follow the illustrative diagram on the next page SECTIONV Page 9 of 16 Washer Diluter BT 1000 2000 PLUS Reading Station ITEM POS QTY OTA DESCRIPTION DESCRIZIONE Diluter Pinch Valve Tube Aspiration Tube Washing Module Tube Washing Module Peristaltic Pump Cartridge ANNUAL MAINTENANCE KIT P N 662 2001 BT 1000 2000 PLUS KIT MANUTENZIONE ANNUALE cod 662
13. 0 will be added to the left to reach the preset data dimension In case the length of the numerical values string is longer than the data length then the string will be truncated to match the data length It is not possible to use variables as parameter lt Length gt Example Stringn Hello Word 40 Stringn Variable1 0x10 SECTIONIV Page 8 of 18 Char Identifies a single character or single byte Set Syntax Char lt Character gt Example Char H Char 20 Char 0x10 Char STX Identifies the beginning and the end of the group of repetitive commands Syntax Set Begin lt Name of group gt Begin repetitive group Set End lt Name of group gt End repetitive group Note Actually the ANALYSESDATA is the unique SET present which identifies the analysis in transmission reception Only one command SET BEGIN and one command SET END can be present in a script A script must always contain the command SET The variable PATIENTNUMBERTEST must be present before the command SET SECTIONIV Page 9 of 18 COMPILATION ERROR One or more errors due to incorrect script writing or the system error may show up during compilation of a script The compiler shows the error code the description of error and the line where it has been detected The following table shows the error codes description and the possible causes Error Code Description Possible Causes 1 Unknown command An invalid command h
14. Computer Module The following different operations are required for the removal and Replacement of each particular device 1 Hard Disk The hard disk item 8 Figure 5 12 is located under the Computer Module Disconnect the cables fro the hard disk Remove the 2 mounting screws on the supporting bracket and dislodge the hard disk 2 DVD CD ROM amp Floppy Disk Unscrew the 4 mounting screws remove the power inlet panel item 11 Figure 5 12 from the analyzer rear Remove the 4 screws securing L shaped bracket which holds DVD Floppy Drive Main board and Peripheral Devices Computer Module Disconnect the cables from the hard disk item 8 Figure 5 12 Remove the 4 mounting 2 screws on the left and right of the module see item 7 Figure 5 12 SECTION V Page 15 of 16 Now it is possible to slide open the module to access the motherboard and the peripheral boards etc 4 Computer Power Supply The power supply 9 of the computer is located above the computer module over the LCD display remove the power supply it is necessary to remove the 2 screws securing the supporting metallic bracket 5 TFT LCD Display Panel First of all remove the computer module 7 and the computer power supply 9 and then unscrew 4 screws on the panel corners to remove the LCD display panel 6 Analyzer Power Supply This power supply 0 can be easily removed by unscrewing 3 mounting scre
15. IX Page 7 of 7 SECTION X POWER SUPPLY AC DC MODULE 10 1 Power Supply Lambda CA400 Series Alpha power supply AC DC CA400 12MIN 24D 24DRP Universal Input voltage range 85 264 VAC Frequency 47 Hz to 63 Hz CONFIGURED OUTPUT VOLTAGES Output Voltages preset Adjustment Range Output Current 12 Volts 12 Volts 8 Amp 24 Volts 24 Volts 8 Amp 24 Volts 18 29 Volts 8 Amp 10 2 Inhibit Option INH The inhibit option is provided as a board inside the module that can reduce the output voltage of 12 Volts to less than 0 5 Volts by application of a TTL compatible control signal This circuit is inhibited through the reset signal In this way the voltage supply to the analyzer lamp is cut off while interrupting power to the solid state relay located on the Control Board 15 04431 01 thus interrupting the mains voltage supply to the peripheral SECTION X Pagel of I SECTION XI THE VACUUM SYSTEM WASTE PROBE P N 662 0788A Figure 11 1 11 1 The Waste Pump and the Waste Probe The integral pump system of BT 1000 and BT 2000 PLUS transfers the waste liquid from analyzer s reaction cuvettes and washing system through the rear mounted waste probe into the external waste disposal container For safety reasons the waste probe is equipped with an overflow liquid level sensor which is actuated when the external waste container is full Whenever the external waste container is full t
16. Normal control b3 Normal control Ten sample readings were repeated and the factor K carry over was calculated for each series with formula b1 b3 a3 b3 x100 The same test was repeated using potassium dichromate 17 5 gr l in sulphuric acid 0 01N reading 340 700 nm as sample and sulphuric acid 0 01N as reagent The test was calibrated with 100 value and the samples used were of 100 and 25 value Results The calculations originated in a carry over for the glycaemia between 0 3 to 0 5 and for dichromate between 0 5 to 0 9 2 13 9 Sample Evaporation Consideration was given to the samples contained in 10 ml tubes and capsules of 1 8 ml to evaluate the sample evaporation The examined samples were control serum pool sulphuric acid 0 01N with PEG 6000 50 gr l and sulphuric acid 0 01N The solution of acid with the PEG has almost the viscosity of serum Six milliliters each of the three sample solutions was dispensed into the tubes while 500 ul of each sample solution was dispensed into the capsules The ambient temperature conditions varied from 21 C and 45 R H to 21 C and 47 R H Immediately the six samples were weighed on a precision balance Afterwards successive readings were executed every 15 minutes for a total of 4 hours that is four times more than the sample usually remains on the plate During the intervals for weighing the samples were on the plate in the analyzer without anti evaporation cover and in normal o
17. 1 bit The digital impulses pass through the U10 Gate 7415132 with Smith trigger function before reaching the external microcontroller for final processing The two multiplexers are addressed by the uC764 master logic board with 4 bits multiplexer The electronic circuit of the photometer is powered by 12 Volts for the analog circuits and 5 Volts for the digital circuits A 16 conductor flat cable is used for connecting the photometer to the master logic board The calibration of each channel is performed at the factory to observe the following requirements clean and transparent cuvette filled with distilled water new halogen lamp and sufficient warm up time The diagnostic program is used for verification and any correction if necessary Each channel must generate an output signal between 4 Volts and 8 Volts theoretical The voltage values are not visible in the diagnostic program but the integration values are equivalent if the conversion factors are assumed as reference In practice the integration values should be between 14 000 and 28 000 points for all of the channels This range takes into account the tolerances necessary for different lots of lamps and their related exhaustion SEMI REFLECTING LIGHT BEAM MIRRORS INPUT REFLECTED LIGHT INTERFERENCE PHOTODIODE ARRAY Figure 6 8 PHOTODIODE DETECTORS SECTION VI Page 10 of 11 6 9 1 Technical Specifications of the Photometer Optics
18. D oett osten Identifies the type of patient in this case Serum 003 Identifies numbers of reports ited chi First test code 000 000 GLI test result BUN i uxo Second test code 0010 10 BUN test result on ec Third test code 00100 0 COL test result enn Identifies the Check Sum lt gt This character ends communication 4 4 Calculation of Check Sum This procedure calculates a Control code in accordance with the transmitted or received data An algebraic sum of ASCII values of all the sent characters is executed For example the character A has ASCII value 65 0x41 Consequently the module 256 of the found value is executed balance of dividing the value by 256 This is the Check Sum to be sent 4 5 Wiring Diagram of Interface Cable 1 2 3 4 5 7 8 9 bt TARGA Plus RS 232 SECTION IV Page 4 of 18 1 2 3 4 5 6 7 8 9 oad oo A Ww M H bt TARGA Plus IBM RS 232 SECTION IV Page 5 of 18 4 6 VARIABLE SERIAL PROTOCOL Introduction The variable serial protocol has been designed to provide the user with possibility to personalize the transmitted and received data from the analyzer The user can transmit or receive in addition to preset data patient code analysis code results etc also the simple text strings and or characters in order to meet the personal requir
19. H20 TUBE WITH FILTER Vacuum Pump Module Figure 11 3 SECTION XI Page 3 of 4 Vacuum Pump P N 08 04865 01 Figure 11 4 en TUBE WITH FILTER P N 08 04716 01 Figure 11 5 SECTION XI Page 4 of 4 SECTION XII MEASUREMENTS amp CALIBRATIONS 12 1 General The BT 1000 and BT2000 PLUS chemistry analyzers have a general diagnostic program exclusively for the technical assistance personnel The access to this program is not protected by a password but an absolute familiarity with the analyzer is a prerequisite The following functions can be performed in this program 1 Verification of operation and stability of the photometer 2 Testing hydraulic functions including sampling washing and emptying of cuvette 4 Verification and programming of the barcode 5 Program of stress To access the diagnostic program click on the Analyzer button and then click on the General Diagnostic button for displaying the General Diagnostic page Now click on the desired functions 12 1 1 Photometer Calibration and Specifications The calibration consists of amplification adjustments of each channel so that these are appropriate to variables present in the photometer The interference filters and the semi reflecting mirrors may have tolerances of 30 which must be compensated to avoid saturation of the amplifiers SECTION XII Page I of 11 The calibration procedure for amplifications remains the same
20. MEASUREMENTS AND CALIBRATIONS TITLE SECT GENERAL dernest Bildet XII PHOTOMETER CALIBRATION AND SPECIFICATIONS XII PHOTOMETER CALIBRATION XII VERIFICATION OF ANY PROBLEMS DURING THE READING XII CUVETTES CALIBRATION AND TEMP CONTROL SPECS XII CUVETTES CALIBRATION XII REFRIGERATION SYSTEM CALIBRATION AND SPECS XII REFRIGERATION SYSTEM CALIBRATION PROCEDURE XII TEMPERATURE MONITORING XII BARCODE CALIBRATION AND SPECIFICATIONS XII PROGRAMMING OF SERUM REAGENT BARCODE XII BARCODE MECHANICAL CALIBRATION PROCEDURE XII VACUTAINER SENSOR CALIBRATION AND SPECS XII STRESS ANALYZER epe tpud t Rn de ee Pre RENE XII SECTION XIII TROUBLE SHOOTING TITLE SECT INTRODUCTION Leke une Savan qtu peracto ee mee fortu CUVETTES TEMPERATURE seem XIII REAGENTS CHAMBER XIII POWER SUPPLY PROBLEMS eo hp gone cnr XIII POWER UP FROBLEMS Harnes aksn ener rehenes ted XIII THE MOUSE AND XIII RESET ERRORS SERUM PLATE REAGENT TRAY XIII DIEUTER SAMPEIN G ARM nitride Ete tins INTRODUCTION COMPUTER MODULE TROUBLE XIII SHOOTING
21. SECTION V Page 6 of 16 H 0 RESERVOIR LIQUID LEVEL PERISTALTIC PUMP DILUTER WASHER PINCH VALVE DILUTER PINCH VALVE WASHER CUVETTE PLATE WASHING LIGHT SOURCE PISTON LAMP PHOTOMETER SN E CNN 14 KI PELTIER MODULE 3 E 2 CUVETTE MOTOR READING STATION MODULE Figure 5 4 5 4 Repair or Replacement of the Reading Station Module It is possible to access nearly all the parts of the reading station module for the maintenance and the repair The analyzer must be turned off prior to repair or Replacement Only in the case of the Replacement of Peltiers located at the bottom of module It will be necessary to remove this module The following components are easily accessed without removal of the module 1 Replacement of Halogen lamp 2 Replacement of all the tubes of the hydraulic circuit 3 Replacement or maintenance of diluter 4 Replacement of washing piston 5 Replacement and inspection of reading cuvettes 6 Replacement of photometer SECTIONV Page 7 of 16 5 4 1 Replacement of the Cuvette Peltier For the Replacement of the Peltiers it is necessary to remove the 3 mounting screws see exploded view of the reading station To facilitate the removal of a screw located inside it is necessary to remove the front cabinet It is important to disconnect all of the cables and tubing before removing the station Each Peltier group contains a fan and heat sink and is securely att
22. Sample Select only one of these NO YES SAMPLE CONTROL Sample Control Sample Control Sample Control Sample Control Sample Control Sample Control Identifies a Known or Unknown Control Select only one of these Unknown Known Unknown Known Unknown Known Unknown Known Unknown Known Unknown Known Identifies Control Level Select only one of these ON LOW NORMAL ABNORMAL Level 1 Level 2 Level 3 Level 1 Level 2 Level 3 Level 1 Level 2 Level 3 Level 1 Level 2 Level 3 0 Identifies if it is a Clone Select only one of these 0 1 0 14 N NOCLONE CLONE Normal Clone Normal Clone Normal Clone Normal Clone SECTION IV Page 13 of 18 TABEL 2 INTERNAL VARIABLES Variables Decimal Hexadecimal NUL 00 0x01 SOH 01 0x02 STX 02 0x03 ETX 03 0x04 EOT 04 0x05 ENQ 05 0x06 ACK 06 0x07 BEL 07 0x08 BS 08 0x09 TAB 09 OxOA LF 10 OxOB VF 11 0x0C FF 12 0x0D CR 13 OxOE SO 14 OxOF SI 15 0x10 DLE 16 0x12 DC1 17 0x13 DC2 18 0x14 DC3 19 0x15 DC4 20 0x16 NAK 21 0x17 SYN 22 0x18 ETB 23 0x19 CAN 24 Ox1A EM 25 0x1B SUB 26 Ox1C ESC 27 Ox1D FS 28 Ox1E GS 29 Ox1F RS 30 0x20 US 31 0x21 SECTIONIV Page 14 of 18 SCRIPT EXAMPLES The examples outlined here are the
23. THAT THE VOLTAGE ON THE REAR PANEL LABEL MATCHES THE LOCAL LINE VOLTAGE 6 TO GUARANTEE SAFETY THE SYSTEM MUST BE GROUNDED THE WIRES IN THE MAINS POWER CORDSET ARE COLORED IN ACCORDANCE WITH THE FOLLOWING CODES GREEN AND YELLOW EARTH BLUE NEUTRAL BROWN LIVE THE WIRE COLORED BLUE IS INTERCHANGEABLE WITH WIRE COLORED BROWN SECTION A Page 3 of 11 10 11 12 13 14 15 16 17 18 REPLACE FUSE AS MARKED PRIOR TO THE REMOVAL OF ANY FUSE TURN POWER OFF AND UNPLUG THE CORDSET FROM THE WALL TO REDUCE THE RISK OF ELECTRIC SHOCK DO NOT REMOVE ANY PANEL OR COVER UNDER POWER ON CONDITION BEFORE REMOVING ANY PANEL OR COVER TURN POWER OFF AND UNPLUG THE POWER CORDSET FROM THE WALL FOR OPERATING SAFETY DO NOT INSTALL THE SYSTEM IN A LOCATION WHERE IT WILL BE EXPOSED TO HEATING EQUIPMENT OR RADIATORS DIRECT SUN LIGHT OR ANY OTHER SOURCE OF EXTREMELY HIGH TEMPERATURES DO NOT OPERATE THE SYSTEM IN THE PRESENCE OF FLAMMABLE FLUIDS OR GASEOUS ATMOSPHERE DISINFECTING AGENTS CLEANING AGENTS ETC DUE TO POSSIBLE FIRE OR EXPLOSION DO NOT KINK BEND LAY OBJECT ON OR OTHERWISE DAMAGE OR RESTRICT CABLES BE SURE THAT THE POWER SWITCH ON THE BACK PANEL OF SYSTEM IS OFF WHEN PLUGGING IN OR REMOVING THE POWER CORDSET FROM A WALL OUTLET TURN OFF THE MAINS POWER SWITCH WHENEVER THE SYSTEM IS NOT IN USE THE SYSTEM IS SWITCHED OFF BUT NOT ENTIRELY TO TURN OFF POWER TO WHOLE SYSTEM UNPLUG POWER CORD FR
24. TO BE CURRENT AT THE TIME OF PUBLICATION BUT IT IS PROVIDED WITHOUT WARRANTY OF ANY KIND AND WE ASSUME NO RESPONSIBILITY WITH RESPECT THERETO DANGER TO AVOID ACCIDENTAL CONTAMINATION WITH CHEMICALS PATHOLAGICALS AND MICROBIAL CONTAMINANTS USE SUITABLE GUARDS AND OR PERSONAL PROTECTION WHEN HANDLING CHEMICALS SERUMS REAGENTS ETC THE SAFE DISPOSAL OF THE WASTE MATERIAL IS THE RESPONSIBILITY OF THE USER ACCIDENTAL INJECTION OR PRICKING OF THE SKIN WITH ANY SHARP OBJECT NEEDLES ETC MAY CAUSE A PROLONGED AND VERY PAINFUL LOCAL ANTIMICROBACTERIAL INFLAMMATORY REACTION EVERY PRECAUTION SHOULD BE TAKEN TO AVOID SUCH ACCIDENTS BEFORE SHIPPING THE INSTRUMENT OR ANY REPAIR PARTS ELIMINATE ANY ORGANIC RESIDUES AFTER TREATING WITH APPROPRIATE DISINFECTANT AND ALSO ELIMINATE THE USED REAGENT BOTTLES IN THE REAGENT CHAMBER IMPORTANT NOTICE THE WARNINGS INFORMATION ITSELF IMPLIES MANY NATURAL AND ACCEPTED PRECAUTIONS THE WARNINGS INFORMATION THEREFORE IS NORMALLY USED TO CALL ATTENTION ONLY TO THE MOST IMPORTANT PRECAUTIONS OR TO THOSE THAT ARE NOT NECESSARILY LISTED WITH THE INSTRUMENT COMPONENTS THE RESPONSIBILITY FOR THE SAFE USE OF THE INSTRUMENT OF BIOTECNICA INSTRUMENTS S p A RESTS WITH THE CUSTOMER THE INSTRUMENT SHOULD BE OPERATED OR SERVICED BY THE QUALIFIED PERSONNEL FAMILIAR WITH LABORATORY PROCEDURES THEY SHOULD ALSO BE FAMILIAR WITH THE NATURE OF THE SUBSTANCES USED IN COMBINATION WITH THE INSTRUMENT AND ANY
25. Ten 10 static channels with semireflective mirrors beam splitters with a light input angle of 90 Interference Filters 340 380 405 436 478 510 546 578 630nm at a 8nm hbw and 700nm with 45nm hbw Photodetectors Silicon photodiodes for UV and visible detection 310nm 700nm Electronics Ten 10 multiplexed amplifiers and A D conversion with 22 bit resolution 6 10 FCC Procedure It is highly recommended to execute the FCC procedure every time an important operation is performed in the photometric system i e lamp replacement photometer substitution and cuvette replacement The transmission of all of the cuvettes is established at 100 after the FCC cycle is completed From this moment on the optimal reference point is determined The software driven FCC procedure is simple and easy to operate This procedure is aimed at compensating the optical path of the cuvettes by assigning each one a correction factor Normally in production the cuvette selection is made with a maximum error of 1 5 96 It is important to pour approximately 4 375gm of Potassium Dichromate solution in 1 000ml of HSO4 0 1N Then dilute this solution 1 100 with H2SO4 0 1N absorbance measurement for this product is 400 units at 340nm Upon request bi chromatic channels at 340 700nm can be selected Once the FCC is completed the system presents the absorbance values of all cuvettes with the correction values per cuvette in percentages The progra
26. averaged For the test 30 readings were performed on the analyzer with two samples at different viscosity with following sample volumes 3 ul 10 ul and 30 ul Outlined below are the averages of the readings obtained manually mABS PEG as reference and viscous samples mABS PEG Sampled The percent error has been calculated using the following formula Vx Vt Vt x100 Where Vx is the average value of samplings performed by the analyzer and Vt is the average value of reference absorbances Results SAMPLE Dichromate PEG 50 gr l SAMPLE Dichromate PEG 100 gr l Solution 3 ul 10 ul 30 ul Solution 3 ul 10 ul 30 ul Average 147 480 1341 Average 138 444 1223 D S 2 5 3 7 12 4 D S 1 8 4 4 8 8 C V 1 7 0 8 0 9 C V 1 3 1 0 0 7 2 13 8 Carry Over in Serum This test is used for verification of any presence and entity of carry over A normal control serum lot number 90141 expiry date 08 02 and Biotecnica pathological lot number 90062 expiry date 06 01 were used The liquid glucose reading 510 700 nm with lot number 09166 and expiry date 09 00 was used as a reagent The test was calibrated by a chemical standard having concentration of 100 mg dl lot number 9018 expiry date 03 02 Six capsules were positioned on the plate containing respectively SECTION II Page 10 of 12 a1 Pathological control a2 Pathological control a3 Pathological control b1 Normal control b2
27. halogen lamp of the photometer 2 12 Photometer Halogen Lamp This lamp is custom made for Biotecnica Instruments S p A It is a halogen lamp with diachronic reflector and a special coating for maximum UV ultra violet emission Beam Angle Watts Volts Rated Life 92 35W 12 2 500 hours theoretical SECTION II Page 5 of 12 For optimal result the lamp can be used for about 1 500 hours The long term use will result in the gradual deterioration of the UV emission 2 13 Fluid Dynamics Mixing Prime Water H2O Supply Liquid Sensor Sensitivity Contamination Sample Viscosity Carry Over and Sample Evaporation 2 13 1 Mixing Verifications Test performed directly on the instrument Instruments Potassium dichromate solution with PEG 100g liter in sulphuric acid 0 01N Twenty 20 tests were performed with or without mixing using 3pul and 10 sample 300ul of reagent aspirating dichromate with PEG as sample and utilizing sulphuric acid as reagent The reading was performed at 340 700nm after an incubation lasting 30 seconds The O D of solution were 0 150 ABS at e 0 482 ABS at 1011 Results Test with without mixing 1 3 Test with after mixing 0 6 Test with 10ul without mixing 0 7 Test at 10ul after mixing 0 4 2 13 2 Phenomenon of Dilution of Solutions The hydraulic circuit of the BT
28. in communication setup The sequence is RF Receiver keyboard and the mouse SECTION XIII Page 3 of 10 13 1 6 Trouble Shooting Reset Errors Reset Errors Serum Plate Reagent Tray Diluter Arm SYMPTOMS CAUSES AND CORRECTIVE ACTIONS The analyzer gt after routine boot displays an error message about one of the following modules e Reset error of serum plate e Reset error of reagent tray e Reset error of diluter e Reset error sampling arm etc 1 Inadvertent accidental blockage by operator of one or more modules For correct operation perform a general reset F5 or Reset Icon 2 If the problem persists the problem may lie in the mechanics or electronics of one of the modules The defective module seems to be operating but an error message appears NOTE To diagnose a mechanical or electronic problem is guite simple Generally speaking a mechanical defect produces a partial movement in the device and careful observation of the mechanism during operation one can easily identify the problem The electronic problems are related to motor drivers and the position sensors In case the device is functional but does not stop in correct position the problem in the reset sensor Depending upon the module it may be a photo switch or Hall sensor These specified problems might associate to diluter reagent tray serum plate cuvettes plate and the washi
29. of sensors detecting Vacutainer presence SECTION Page 4 of 8 8 3 2 Serum Plate Controller Module 15 04422 01 This electronic module consisting of a board inside a metallic container Figures 8 4 amp 8 7 controls all the functions of the sampling system see Figures 8 4 amp 8 5 The connection to other modules is I2Cbus cable Like all the other modules the same connector supplies 5Volt 24Volt and has two SDA and SCL wires The functions of this board are Serum plate stepper motor driver RS232 interface for barcode reader reading of digital signals of the vacutainer sensors and Hall sensors located around the serum chamber 8 3 3 Vacutainer Sensor and Hall Sensor Board 15 04280 01 This supplementary circuit Figures 8 3 and 8 5 is affixed to the left of the serum chamber It incorporates an optoelectronic sensor assy for the detection of vacutainer tubes This reflection sensor consists of an impulse driven infrared LED emitter and an infrared photodiode receiver which receives reflected light from the vacutainer A comparator amplifier U2 converts analogic signal to low or high digital signal When the tube is present the low signal indicates the detection of vacutainer confirmed also by the glowing green LED on the wall of the serum chamber Close by there is the Hall sensor assy for serum plate reset and a led indicator for the sample tube positioning The trimmer TR1 is used for the initial calibration
30. of the system All the preceding circuits are connected to the serum Plate Controller Module 15 04422 01 through flat cable connectors 8 4 Barcode Scanner This is a high quality laser scanning amp CCD module Figures 8 3 8 5 8 6 amp 8 8 It is connected to the Serum Plate Controller Module through the serial port It can automatically read 6 different types of codes It is also possible to reprogram the scanning modules with additional codes during installation Bt 2000 has barcode scanning modules for the reagent module and the serum module Bt 1000 has no barcode scanning modules SECTION VIII Page 5 of 8 8 5 The washing funnel Figures 8 3 8 5 and 8 6 is firmly secured to the left side of the serum chamber This is also the homing reset position of the sampling arm needle The hydraulic circuit is washed through this funnel The peristaltic pump located on the reading station pumps water through the whole circuit A volume of about 2 ml is sufficient for ensuring an optimal wash of the needle externally when it is almost totally immersed in the washing funnel The washing funnel is emptied by a membrane pump Figure 8 6 which goes into operation at appropriate moments The waste fluid in the funnel is discharged through a PVC tube to the waste outlet A check valve Figures 8 6 and 8 10 on the funnel side is connected in series to the circuit to avoid any flow problems with the main vacuum Washing Funnel pump
31. on the rear ARM LOCATION VACUTAINER SENSOR WASHING FUNNEL HALL SENSOR BARCODE MODULE SERUM CHAMBER BASE PLATE SERUM PULLEY SERUM CHAMBER BASE PLATE DRIVE BELT SERUM MOTOR SERUM PLATE SERUM MODULE TOP VIEW A SERUM PULLEY SERUM MODULE FRONT VIEW SERUM MODULE Figure 8 3 SECTION VIII Page 6 of 8 CONTROLLER MODULE 15 04422 01 BALL BEARING HEAD BOARD 15 04422 01 I CBUS lCBUS SERUM MOTOR VACUTAINER SERUM PLATE CONTROLLER MODULE Figure 8 4 SERUM PLATE 9 MAGNET FOR HALL SENSOR WASHING FUNNEL VACUTAINER SENSOR SSN SERUM PLATE CONTROLLER MODULE SERUM PULLEY BARCODE MODULE SERUM MOTOR SERUM MODULE EXPLODED VIEW Figure 8 5 SECTION VIII Page 7 of 8 Various Devices Serum Module m 3 SERUM FUNNEL CHECK EE CONTROLLER MODULE BARCODE YN MODULE SERUM DEVICES Serum Controller Module Figure 8 6 Figure 8 7 SERUM PULLEY BARCODE mo 1 MODULE SERUM MECHANISMS SERUM PLATE Figure 8 8 Figure 8 9 ROUX WE SERUM 54 gt CIRCULAR LEVEL FUNNEL CHECK VALVE Serum Chamber Top View Figure 8 10 Figure 8 11 SECTION VIII Page 8 of 8 SECTION IX COMPUTER MODULE COMPUTER MODULE Figure 9 1 COMPUTER MODULE OPENED Figure 9 2 SECTION IX Page 1 of 7 00000000 09000
32. panel 11 are located to the left of Computer Module Product enhancement resulting from new innovations in technology and from our continuing quality improvement effort may necessitate changes in this configuration in the future However any eventual changes will be fully compatible with the previous version A connecting cable composed of lead conductors of the serial port and the 5 Volt supply interconnects the Computer Module and the process electronics eoe CONNECTORS Figure 9 4 9 1 2 MOTHERBOARD CONNECTORS 1 PS 2 Mouse Port Connector 6 pin female 4 1 PS 2 Keyboard Port Connector 6 pin female B 3 Universal Serial Bus USB connectors O three 4 pin female for Data Bus 2 Serial Ports Connector D com1 9 pins amp com2 9 pins 1 Parallel Port Connector 25 pin female for Printer An important feature in implementing a silent system is the Auto Fan Off function The power supply to the fan will automatically switch to a standby mode as needed This function reduces both the energy consumption and system noise SECTION IX Page 3 of 7 9 1 3 LCD MODULE This module is color active matrix LCD module incorporating amorphous silicon TFT Thin Film Transistor It is composed of TFT LCD panel Driver ICs control circuit and power supply circuit and a backlight unit The display module is connected to the LCD Interface through a flex cable The interface board is inserted into the appropriate slot on the mothe
33. plate OxOE Too many analysis for the patient For example to send a patient with code 000000000000001 serum type and with analysis GLI BUN and COL onto the STATS list then one must send the following sequence of characters excluding initial sequence STX lt gt ACK 000000000000001RSN0003GLI BUN COL 134 lt EOT gt Where 000000000000001 Patient code N eee ime Identifies STATS list see tees Identifies the type of patient in this case Serum lt Identifies that the patient is not a clone 00 5 asa aa Maton Unknown position the analyzer will insert the patient in a convenient position identifies the number of test to be executed GLI BUN COL Test codes observe the space after each code to reach the 4 characters limit 194 oie Identifies the Check Sum lt gt This character ends communication SECTIONIV Page 2 of 18 4 3 Results reception There are three commands for receiving reports from the analyzer Ross Reception of next available report Is Reception of the last report sent in case of reception problems Reception of the first available report in case desires to receive again all the reports The commands R L and A require standard communication or the procedure STX lt gt ACK and the character EOT to end communication As a response to one of these three comma
34. sensor or the master power board or the chip temperature xxx Refer to Measurement and Calibration procedures for the reading station outlined in the Section XII to find out the defective device After replacing the temperature sensor there may be temperature fluctuation of 0 5 C In this case make adjustments as per calibration procedure 3 A defective Peltier module will cause temperatures to be lower or near ambient temperature Check if there are one or more defective Peltiers Even one defective Peltier can stop the temperature monitoring system SECTION XIII Page I of 10 13 1 2 Trouble Shooting Reagent Chamber Temperature REAGENTS CHAMBER TEMPERATURE SYMPTOMS CAUSES AND CORRECTIVE ACTIONS The base of the reagent chamber does not reach the set lower temperatures 5 20 C or some times seems to be heating In some cases excessive cooling with ice formation 1 2 3 Defective Cross flow blower causing temperature Verify and replace if necessary Interrupted Peltier causing refrigeration failure Verify and replace defective Peltier Defective temperature sensor or reagent control board causing overheat or ice formation Verify and replace the defective part high 13 1 3 Trouble Shooting Power Supply POWER SUPPLY PROBLEMS SYMPTOMS CAUSES AND CORRECTIVE ACTIONS 1 The analyzer turns
35. singularly or combined snapped pack for single or dual reagent analyses The reagent chamber cover is removed from the top for unobstructed access to reagents SECTION VII Page 1 of 6 7 2 Reagent Plate Controller Module 15 04313 01 The electronic board 15 04313 01 for the reagent plate management is housed in a convenient metallic container This module is located on the rear of the reagent chamber and is easily accessible for service or repair through the rear panel of the analyzer The electronic devices on this board have the following functions a Reagent plate drive motor control b Zero position sensor control c Reagent plate temperature control d Barcode reading and interface only on BT2000 PLUS The Reagents Plate Controller Module 15 04313 01 is controlled by uC87LPC764 This micro controller manages the stepper motor through two opto couplers U3 amp U4 which isolates the power driver A1 from the digital circuit The reagents chamber temperature is controlled through a U6A comparator and the Q1 transformer The control temperature is fixed at about 5 C by using the remote control of the power supply Lambda Alpha 400W The J2 connector connects the power supply to the controller circuit of the reagent plate The Q2 transistor functions as a switch to interrupt power supply to the Peltiers through the uC764 pin 14 whenever requested by the software The circuit for the control of the Peltier voltages has an
36. the password lt lt administrator enzo gt gt 6 Enter Control Panel System Hardware and then Device Manager to verify the correct installation of all the devices Eventually remedy the causes of any conflicts and if necessary install an appropriate updated driver for any unidentified device Audio additional serial port and etc 7 Install application software of the external peripherals i e printer UPS etc 8 Install application software of BT 2000 PLUS SECTION XIII Page 9 of 10 BOOTSTRAP COMPUTER MODULE TROUBLE SHOOTING SYMPTOMS CAUSES AND CORRECTIVE ACTIONS After power on and Enter Control Panel Users and Passwords remove the during the bootstrap X mark from the box corresponding to the lt lt User must process password enter name and password to use this computer and to request window change passwords and other settings gt gt appears on the display Possible problems during reinstallation of hardware devices i e additional serial boards touch screen etc Correct drivers have not been installed Previously a board by another manufacturer was installed and is still present in the system even if physically disconnected Note In the case of serial board two new gates COM3 and COM4 are assigned to replace COM5 and COME In such a case it is necessary to enter the Advanced Settings of the appropriate serial communic
37. the customer s expense Product enhancements resulting from our continuing quality improvement efforts may necessitate changes in specifications without notice BIOTECNICA INSTRUMENTS S p A VIA LICENZA 18 00155 ROME ITALY TEL 39 06 4112316 FAX 39 06 4103079 SECTION A Page 2 of 11 WARNINGS ALTHOUGH THE BT 1000 or BT2000 PLUS ANALYZER SYSTEM USES HIGH PERFORMANCE COMPONENTS WHICH PROVIDE A HIGH DEGREE OF SAFETY IT IS ESSENTIAL THAT THE SERVICE ENGINEER TAKES THE USUAL PRECAUTIONS TO SAFEGUARD HIS SERVICE PERSONNEL WE ONLY GUARANTEE THE WORKMANSHIP AND MATERIALS OF OUR PRODUCTS IT IS THE DUTY OF THE SERVICE ENGINEER TO TAKE CARE FOR SAFE OPERATION AND NO AMOUNT OF WARNINGS CAN TAKE THIS PLACE OF SUCH CARE THE FOLLOWING WARNINGS WILL AID THE SERVICE PERSONNEL TO PROVIDE ADEQUATE SAFEGUARDS TO ASSURE SAFE TROUBLE FREE PERFORMANCE 1 BEFORE SERVICING THIS SYSTEM BE SURE TO READ THE SERVICE MANUAL THOURALLY AND CAREFULLY AFTERWARDS KEEP IT HANDY FOR FUTURE REFERENCE 2 TAKE SPECIAL CARE TO FOLLOW THE WARNINGS AND CAUTIONS INDICATED ON THE SYSTEM REAR PANEL AS WELL AS IN THE SERVICE MANUAL 3 SERVICING OF THIS SYSTEM SHOULD BE RESTRICTED QUALIFIED APPROVED SERVICE PERSONNEL 4 SLOTS AND OPENINGS IN THE CASE BACK PANEL AND BOTTOM ARE PROVIDED FOR VENTILATION THIS ENSURE RELIABLE OPERATION OF THE SYSTEM AND TO PROTECT IT FROM OVERHEATING DO NOT BLOCK OR COVER THESE OPENINGS 5 BEFORE SERVICING CHECK
38. to verify the inconsistency of an average measurement rather than the absolute value that is in theory approximately 2 Ohm per Peltier Generally a defective Peltier presents a partial interruption with values of some 10 Ohms which are sufficient to drastically reduce the current flow in the series of Peltiers therefore generating a diminished performance of the refrigeration group Unsolder the leads of the defective Peltier unscrew the 2 screws on the metallic disc securing the Peltier element and remove it Substitute the damaged Peltier with a new Peltier module using thermal grease for dissipation Make sure to take into account the proper polarity see figures 7 1 7 8 and 7 9 in Section VII An incorrect assembly may affect the system performance Carefully tighten the screws ensuring that these are not over tightened so as to avoid damage to the ceramic elements of the Peltier After assembling all of the devices it is not important to recalibrate the temperature control SECTION V Page 5 of 16 5 3 2 Replacement of the Reagent Plate Transmission Belt For this operation it is necessary to remove the case computer box and the front cover Figure 7 10 in Section VII Remove the round refrigeration chamber in aluminum by unscrewing the 16 mounting screws Observe the photo sequence for disassembly details Loosen the screws of drive motor assembly plate located on the top left corner and slide it slightly inward to loosen t
39. 00 amp BT2000 PLUS TITLE ARM L ASSY ARM WITH HEATER READING PLATE ASSY READING STATION REAGENTS MODULE ASSY BT 2000 PLUS ONLY BT 2000 PLUS SERUM MODULE BT 2000 PLUS ONLY SERUM MODULE BT 1000 ONLY SERUM PLATE ASSY BT 2000 PLUS ONLY SERUM PLATE ASSY BT 1000 ONLY FRONT COVER ASSY BT 2000 PLUS ONLY FRONT COVER ASSY BT 1000 ONLY ANALYZER BASE ASSY BT 2000 PLUS ONLY ANALYZER BASE ASSY BT 1000 ONLY DECK ASSY BT 2000 PLUS ONLY DECK ASSY BT 1000 ONLY LIQUID PUMP SYSTEM POWER SUPPLY PANEL ARM L REAGENTS MODULE ASSY BT 1000 ONLY BT 1000 INDEX Page 12 of 12 DISCLAIMER BIOTECNICA INSTRUMENTS S p A has an international reputation for product quality and performance The company provides appropriate product information and warnings to the customer in order to assist them in utilization of products in a reasonable and safe manner The service personnel assigned to repair of BT 1000 or BT2000 PLUS chemistry analyzer is cautioned to review all the specific warnings found in this manual In no event shall BIOTECNICA INSTRUMENTS S p A be liable or responsible to such service personnel for any injury incidental or consequential damages It is incumbent upon the service engineer to provide its personnel with a thorough understanding of the instrument operation before field depot repair and maintenance BIOTECNICA INSTRUMENTS S p A reserves the right to revise this manual without notice for any reason This i
40. 000 PLUS SYSTEM PARA TITLE SECT 1 1 EXAMPLES OF THE BASIC FUNCTIONS OF CLIN I 1 2 PHASE 1 CREATION OF THE 1 3 PHASE 2 PLACEMENT OF SAMPLES AND REAGENTS I 1 4 PHASE 3 START UP tas I 1 5 PHASE 4 PROCESS INA HON kivuton peres Goa I 1 6 PHASE 5 READING CYCLE sontaa tetsuo SECTION II TECHNICAL SPECIFICATIONS PARA TITLE SECT 2 1 AMBIENT CONDITIONS OF 2 2 PHYSICAL DIMENSION OF 2000 II 2 3 POWER SUPPLY REQUIREMENTS II 2 4 EMC AND ELECTRICAL SAFETY 2 5 TEST EXECUTION TIMES FOR CLINICAL CHEMISTRY II INDEX Page 2 of 12 PARA 2 5 1 2 6 1 2 6 2 2 7 2 7 1 2 8 2 9 2 10 2 11 2 12 2 13 2 13 1 2 13 2 2 13 3 2 13 4 2 13 5 2 13 5 1 2 13 5 2 2 13 6 2 13 7 2 13 8 2 13 9 PARA 3 1 3 2 3 3 3 3 1 3 3 2 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS TITLE NON TEST EXECUTION TIMES siten a eaters ANALYZER WARM UP REQUIREMENT ANALYZER CUVETTE VOLUMES amp CONSUMPTION FOR RESIDUAL VOLUMES IN THE CONTAINERS CUVETTE TEMPERATURE CONTROL eee REAGENT
41. 1 SE 04897 01 SE 04897 01 SE 04902 01 SE 04902 01 ELECTRONICS SYSTEM TITLE MASTER POWER MASTER POWER LAYOUT MASTER LOGIC MASTER LOGIC LAYOUT READING PLATE INTERFACE READING PLATE INTERFACE LAYOUT ARM LIQUID SENSOR amp EMITTER ARM LIQUID SENSOR amp EMITTER LAYOUT SERUM VACUTAINER R SERUM VACUTAINER R LAYOUT SERUM VACUTAINER L SERUM VACUTAINER L LAYOUT SERUM HALL SENSOR SERUM HALL SENSOR LAYOUT REAGENTS PLATE CONTROLLER REAGENTS PLATE CONTROLLER LAYOUT SERUM PLATE CONTROLLER SERUM PLATE CONTROLLER LAYOUT PERIPHERALS DISABLE PERIPHERALS DISABLE LAYOUT PREAMPLIFIER PREAMPLIFIER LAYOUT PHOTOSWITCH INTERFACE PHOTOSWITCH INTERFACE LAYOUT POWER SUPPLY INTERFACE POWER SUPPLY INTERFACE LAYOUT REFRIGERATOR INTERFACE BT 2000 PLUS ONLY REFRIGERATOR INTERFACE LAYOUT BT 2000 PLUS ONLY ARM CONTROLLER ARM CONTROLLER LAYOUT ANGULAR POSITION ENCODER ANGULAR POSITION ENCODER LAYOUT PUMP DISABLE PUMP DISABLE LAYOUT LIQUID SENSOR LIQUID SENSOR LAYOUT INDEX Page 11 of 12 BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS lt lt BT 1000 amp BT2000 PLUS gt gt SCHEMATICS AND DIAGRAMS SCHEMATIC SE 04436 03 SE 04438 01 SE 04836 01 SE 04838 01 SE 04839 01 SE 04840 01 SE 04843 01 SE 04843 02 SE 04844 01 SE 04844 02 SE 04845 01 SE 04845 02 SE 04847 01 SE 04847 02 SE 04851 01 SE 04851 02 SE 04856 01 SE 04857 01 SE 04872 01 SE 04976 01 SE 05000 01 ELECTRONICS SYSTEM BLOCK DIAGRAMS BT 10
42. 2000 PLUS produces an inevitable dilution of the aspirated reagents as the water column is always present in the tube up to the needle tip and this causes a diffusion effect with reagent and thus producing a slight dilution Some compensations have been made to attenuate this phenomenon whose effects and results are outlined below For a reagent volume of 60 to 500 ul an additional reagent volume of 15 ul is aspirated which is ejected during the needle washing phase This additional volume has the function of attenuating the diffusion of washing solution with reagent SECTION II Page 6 of 12 For reagent volumes of less than 60 ul the aspirated reagent volume is automatically increased by 25 2 13 3 Washing Solution Supply The analyzer BT 2000 PLUS utilizes distilled water mixed with a surface active agent surfactant for all the washing and sampling operations The operator can use any container of desired dimensions for the washing solution The container can be positioned at a considerable distance from the analyzer as a powerful pump is used for constant supply of washing solution to the additional reservoir of the analyzer The maximum permissible connecting tube distance between the analyzer and the water container is 25 meters horizontally and 6 meters vertically This data is valid for a flexible plastic tube with inside diameter of 6 mm max 2 13 4 Liquid Sensor The liquid sensor incorporates a high frequency electroni
43. 2001 BT 1000 2000 PLUS 1 2 1 Aspiration Tube Washing Module 5 1 Tubular Filter 2634 Water Container 6 Sampling Needle SECTION Page 10 of 16 5 4 4 Replacement or Maintenance of Diluter Even though it is unlikely that the diluter will require Replacement its removal for service is quite simple Turn off all power to the instrument before servicing The diluter is secured through 4 screws on its flange and 2 connectors for electrical circuitry The maintenance involves yearly cleaning of the piston and the transparent Plexiglas chamber To perform maintenance disconnect the fluid tubing Unscrew the 2 screws located on the front of the diluter and carefully remove the Plexiglas chamber To clean the piston remove the black o ring from the chamber Use a soft lint free cloth for cleaning the piston Wash the Plexiglas chamber and the inside of the o ring with distilled water Place the o ring into the chamber and gently replace the chamber onto the diluter drive Firmly secure with the 2 screws On the rear of Plexiglas chamber there is a special o ring that must be substituted every 3 000 000 3 million cycles 5 4 5 Replacement of the Washing Piston Turn off the analyzer prior to replacing the washing piston Push the washing piston carriage fully upward to facilitate the piston removal Remove all of the silicone tubes Gently unscrew the piston from the plunger stem by turning clockwise In case of
44. DONE A FEW MINUTES AFTER THE MAINS POWER SWITCH HAS BEEN TURNED OFF AND THE POWER CORDSET UNPLUGGED FROM THE WALL OUTLET LAMP MAY BE HOT DURING OPERATION ALLOW LAMP TO COOL BEFORE HANDLING DO NOT TOUCH THE LAMP WITH BARE HANDS INSTALL WITH PROTECTIVE SHEATHING ON BULB THEN REMOVE SHEATHING SINCE THE LAMP RADIATES ULTRAVIOLET RAYS WHICH ARE HARMFUL TO THE EYES DO NOT VIEW THE OUTPUT DIRECTLY DO NOT PLACE THE SYSTEM ON AN UNSTABLE CART STAND OR TABLE THE SYSTEM MAY FALL CAUSING SERIOUS INJURY TO USER AND SERIOUS DAMAGE TO THE APPLIANCE PLACE THE SYSTEM ON A STABLE VIBRATION FREE LEVEL TABLE OR CART DO NOT OVERLOAD ACCESSORIES POWER OUTLETS AND EXTENSION CORDS AS THIS CAN RESULT IN FIRE OR ELECTRIC SHOCK DO NOT OIL ANY PART OF THE SYSTEM DO NOT ATTEMPT TO REMOVE ANY PANELS OR COVERINGS WHILE THE SYSTEM IS IN OPERATION RELEASE THE PERISTALTIC PUMP TUBING WHENEVER THE SYSTEM IS UNUSED FOR LONG PERIODS OF TIME IF IT IS NOT BE USED FOR SOME TIME THE PINCH VALVES TUBING SHOULD BE RELEASED USE ONLY ORIGINAL TUBING REPLACEMENTS SIX MONTHLY P N 662 2000 AND ANNUAL P N 662 2001 MAINTENANCE KITS DO NOT USE CONVENTIONAL TUBING THIS WILL CAUSE MALFUNCTION OF THE SYSTEM AFTER OPERATION SERVICING COVER THE SYSTEM WITH A PROTECTIVE PLASTIC OR CLOTH SHEET SECTION A Page 5 of 11 THE CAREFUL OBSERVATION OF THE PROCEEDING WARNINGS SHOULD RESULT IN A LONG AND SATISFACTORY PERFORMANCE THIS INFORMATION IS BELIEVED
45. E 44 22 222 3 4 SYSTEM ELECTRONICS beds 3 5 EXAMPLE OF THE CONFIGURAZIONE SECTION IV SOFTWARE PARA TITLE SECT 4 1 GENERAL miseksi kde toma epi audeat nie ex etu IV 4 2 STANDARD PROTOQCOL eene IV 4 2 1 PATIENT TRANSMISSION nis depre p eoe ore s IV 4 3 RESULTS RECEP TON perserne nee IV 4 4 CALCULATION OF CHECK SUM ee IV 4 5 WIRING DIAGRAM OF INTERFACE IV 4 6 VARIABLE SERIAL IV 4 7 SERIAL COMMUNICATION TEST PROGRAMS IV 4 8 PROGRAM IV 4 9 PROGRAM BTPEUS EXE rite ri ier Er tn enis Pes IV SECTION V REPAIR OR REPLACEMENTOF VARIOUS PARTS PARA TITLE SECT 5 1 ACCESS TO VARIOUS 5 5 2 REPAIR OR REPLACEMENT OF THE SAMPLING ARM V 5 3 REPAIR OR REPLACEMENT OF THE REAGENT MODULE V 5 3 1 REPLACEMENT OF REAGENT PELTIER MODULE V 5 3 2 REPLACEMENT OF REAG PLATE TRANSMISSION BELT V 5 3 3 REPLACEMENT OF REAG PLATE DRIVE MOTOR V 5 3 4 REPLACEMENT OF REAG TEMP HALL SENSORS V 5 4 REPAIR OR REPLACEMENT OF THE READING V 5 4 1 REPLACEMENT OF CUVETTES
46. II REAGENTS SYSTEM TITLE GENERAL m REAGENT PLATE CONTROLLER MODULE 15 04313 01 REAGENT REFRIGERATION 5 REAGENT REFRIGERATOR MODULE eene REAGENT CHAMBER icit tine testo eedem tete Rt REAGENT PLAT Eines AE veg d ide REAGENT BOTTLE 20 mL 50 REAGENT DRIVE MOTOR n REAGENT CONTROLLER MODULE eee REAGENT GHAMBER tre EUR S Mq REFRIGERATOR MODULE BOTTOM VIEW REFRIGERATOR MODULE TOP REAGENT CONTROLLER REAGENT CONTROLLER MODULE REAGENT DEVICES einn REAGENT DEVICES re INDEX Page 6 of 12 SECT VI VI VI VI VI VI VI VI VI VI VI VI VI VI PARA 8 1 8 2 8 2 1 8 2 2 8 3 1 8 3 2 8 3 3 8 4 8 5 Fig 8 1 Fig 8 2 Fig 8 3 Fig 8 4 Fig 8 5 Fig 8 6 Fig 8 7 Fig 8 8 Fig 8 9 Fig 8 10 Fig 8 11 PARA 9 1 9 1 1 9 1 2 9 1 3 9 1 4 9 1 5 1000 amp BT 2000 PLUS TABLE OF CONTENTS SECTION VIII SAMPLING STATION TITLE SECT GENERAL Vill SAMPLING ARM MODULE cia Aint Mees Vill ARM CONTROLLER BOARD 15 04765 01 VIII ARM LIQUID DETECTOR EMITTER BOARD 15 04276 01 VIII THE SERUM MODULE RECO ar vista iin VIII SER
47. IMPORTANT NOTICE This preliminary service manual is designed to give all needed information and instructions required for successful repair and maintenance of BT 1000 2000 PLUS analyzer system Every effort has been made to have all the illustrations and informations accurately represent the product and its operations as it actually was at the time this service manual was printed Obviously however so that we may effectively continue to meet the requirements of our clients changes in some items may be made during the life of this manual which on occasion without notice We will highly appreciate any comment or suggestion regarding any errors or missing arguments in this manual Biotecnica Instruments S p A Via Licenza 18 00155 Rome ITALY Tel 39 06 4112316 Fax 39 06 410 3079 E mail research Mbiotecnica it BT1000 amp BT2000 PLUS Technology Advanced Random Generation Analyzer System SERVICE MANUAL MANUAL 1 0 RELEASED ON January 21 2003 CE This product conforms to the safety reguirements of the Directive 89 336 EEC It conforms also to Italian Regulations CEI EN 61010 01 and CEI EN 61326 1 EMC The conformity is attested when the eguipment is installed in accordance with the conditions outlined in the manual BT 1000 amp BT 2000 PLUS TABLE OF CONTENTS e DISCLAIMER e WARRANTY e WARNINGS e CONFORMANCE e THE COMPANY PROFILE e PACKING AND UNPACKING THE ANALYZER SECTION INTRODUCTION BT2
48. L P N 395 SURFACTANT WASH CONCENTRATE 4x12 5ml P N 397 FUSE 8AT P N 330 6342B POWER CORDSET M F P N 330 6400 QUARTZ HALOGEN LAMP 12V 35W P N 330 9321 CLEANING TOOL FOR SAMPLING NEEDLE P N 662 0629A N s IN CUBITAINER WITH BOX 10 LITER P N 662 1010 SAMPLE CUP 2mL STD CTRL P N 667 1040 SIX MONTHLY MAINTENANCE KIT P N 662 2000 a ANNUAL MAINTENANCE KIT P N 662 2001 REAGENT CONTAINER 50 mL with CAP P N 667 1073 24 REAGENT CONTAINER 20 mL with CAP P N 667 1074 24 REAGENT CONTAINER 10 mL with CAP P N 667 1076 10 mL CYLINDRICAL TEST TUBE P N 667 1081 SECTION A Page I I of 11 SECTION INTRODUCTION BT 1000 and 2000 PLUS The BT 1000 amp 2000 PLUS are automated analyzers for the determination of clinical chemistry The analyzers can perform 180 210 tests per hour according to version The technical material in this manual is for approved technical personnel with reasonable familiarity with similar instruments only A diligent effort has been made to provide engineering illustrations schematics and other helpful information regarding the analyzer on the following pages to facilitate the task of service personnel The BT 1000 2000 PLUS is a fully automatic analyzer that can perform high volume of analysis on serum or urine samples The test sample is followed continuously upon insertion so the ana
49. LDR not found press any key to restart There is a floppy disk in the floppy disk drive of the computer Remove floppy disk and press any key SECTION XIII Page 8 of 10 BOOTSTRAP COMPUTER MODULE TROUBLE SHOOTING SYMPTOMS CAUSES AND CORRECTIVE ACTIONS During the bootstrap process an error message appears on the screen DISK BOOT FAILURE INSERT SYSTEM DISK AND PRESS ENTER 1 Hard disk cable disconnected Connect the hard disk cable properly 2 Damaged or unformatted hard disk If necessary replace the hard disk Format the hard disk 3 Incorrect configuration in BIOS setup Verify the STANDARD CMOS SETUP and particularly the configuration of the Hard Disk Note Guidelines for installation of the operative system or the application programs on the BT 2000 PLUS complete reinstallation and or substitution of hard disk and or replacement of motherboard is required for motherboards of new generation 4 Configure the startup seguence in BIOS setup with CD ROM drive as the first peripheral the hard disk as second peripheral and the floppy disk drive as the third peripheral Caution After installation of the Windows 2000 PRO operative system restore the original BOOT configuration 5 Install Windows 2000 PRO by starting the computer with the CD and carefully select FAT32 as file system Insert the user name organization name and
50. NECESSARY PRECAUTIONS WHICH SHOULD BE TAKEN IN THE HANDLING USE AND STORAGE OF THE PRODUCTS IN NORMAL USE THE CUSTOMER SHALL BE RESPONSIBLE FOR THE CONTROL AND USE OF THIS INSTRUMENT WHETHER ALONE OR IN COMBINATION WITH OTHER ARTICLES OR SUBSTANCES OR IN ANY OTHER MANNER WHATSOEVER NOTE Products and corporate names appearing in this manual may or may not be registered trademarks or copyrights of their respective companies and are used only for identification or explanation and to the owner s benefit without intent to infringe Windows is registered trademark of Microsoft Corporation IBM is a registered trademark of International Business Machines Intel and Pentium are registered trademarks of Intel Corporation Lambda is an Invensys Company Pharmed d Baydur Vacutainer SECTION A Page 6 of 11 CONFORMANCE IMPORTANT NOTICE EMC CERTIFICATE The analyzer BT 1000 or BT2000 PLUS has passed all the tests relevant to Electromagnetic Compatibility EMC and electrical safety This product received the certificate of compliance with international standards EN 66110 1 and EN 61326 1 from Organismo di Certificazione Europea of Rome Italy accredited and certified certificate number 051 of 21 Oct 1998 by Ministero delle Comunicazione of Italy The test reports xxx and xxx are available upon request CE This product conforms to the safety reguirements of the Directive 89 336 EEC It c
51. OM WALL OUTLET DO NOT ATTEMPT TO ALTER THE SHAPE OF ANY PART OF THE SYSTEM IF THE SYSTEM IS NOT OPERATING PROPERLY AND THE TROUBLE SHOOTING SECTION DOES NOT PROVIDE A SATISFACTORY SOLUTION TO THE PROBLEM THEN DO NOT USE THE SYSTEM UNTIL THE DEFECTS ARE REMEDIED INSPECT ALL ACCESSORIES AND SYSTEM CORDS DO NOT USE IF DAMAGE CAN BE SEEN SUCH AS CUT INSULATION OR OUTER COVERING FRAYED OR BROKEN WIRES CORRODED OR BROKEN CONNECTORS ETC TO REDUCE THE RISK OF FIRE OR ELECTRIC SHOCK DO NOT ALLOW FLUIDS OR ANY FOREIGN OBJECT TO ENTER THE SYSTEM WIPE OFF SPILLS IMMEDIATELY DO NOT USE BENZENE THINNER ANY KIND OF SOLVENTS OR ABRASIVE DETERGENTS TO CLEAN THE CASE CLEAN WITH SOFT DUSTING CLOTH DAMPENED WITH DISTILLED WATER IF NECESSARY USE ONLY NEUTRAL DETERGENT SECTION A Page 4 of 11 19 20 21 22 21 22 23 24 25 26 27 28 29 30 31 32 DO NOT STICK OBJECTS OF ANY KIND INTO THE SYSTEM THROUGH BACK PANEL OR CASE SLOTS AS THEY MAY TOUCH DANGEROUS VOLTAGE POINTS OR SHORT OUT PARTS THAT COULD RESULT IN FIRE OR ELECTRIC SHOCK INSTALL THE SYSTEM IN SUCH A WAY THAT ADEQUATE VENTILATION IS PROVIDED ALL AROUND TO PROPERLY DISSIPATE THE HEAT MAKE SURE ALL FLUID LINES ARE FREE OF KINKS NICKS SHARP BENDS PUNCTURES OR OCCLUSIONS BEFORE INSTALLING ON SYSTEM DO NOT TWIST THE PERISTALTIC PUMP TUBING WHEN PLACING IN THE RACEWAY OF THE PUMP ROLLER EXCHANGING THE HALOGEN LAMP SHOULD BE
52. ORTANT NOTICE THE BIOTECNICA INSTRUMENTS S p A RESERVES THE RIGHT TO MAKE CHANGES IN DESIGN AND SPECIFICATIONS WITHOUT NOTICE EVERY EFFORT HAS BEEN MADE TO PROVIDE ACCURATE TECHNICAL AND PRODUCT INFORMATION THE BIOTECNICA INSTRUMENTS S p A DISCLAIMS RESPONSIBILITY FOR ANY ERROR OR OMISSION REGARDING TECHNICAL AND PRODUCT INFORMATION PRESENTED IN THIS SERVICE MANUAL O 2003 BIOTECNICA INSTRUMENTS S p A ROME ITALY ALL RIGHTS RESERVED HEREIN AND NO PORTION OF THIS SERVICE MANUAL MAY BE REPRODUCED WITHOUT THE PRIOR CONSENT IN WRITING OF THE BIOTECNICA INSTRUMENTS S p A SECTION XIV Page 7 of 7
53. T2000 PLUS 662 2001 ANNUAL REPLACEMENT TUBING KIT BT 1000 BT2000 PLUS 667 1040 SERUM CAPSULE 2 ml 667 1081 10 ml CYLINDRICAL TEST TUBE 14 7 ELECTRONICS MECHANICAL HARDWARE PART NO DESCRIPTION BT 1000 ONLY BT 2000 PLUS ONLY SECTION XIV Page 5 of 7 PART NO DESCRIPTION 330 6450 MAIN SOCKET WITH SWITCH 330 7171 LAMBDA POWER SUPPLY 400W 330 7172 IBM POWER SUPPLY FORTRON 200W 330 9318A INVERTER FOR LCD DISPLAY Schematics on the Last Pages of this Manual SECTION XIV Page 6 of 7 14 8 ORDERING INFORMATION Please contact Biotecnica Instruments S p A for any configuration or special requirement not covered in this manual For technical or ordering assistance start with our convenient ordering check list located in the above paragraph For further assistance don t hesitate to call the Biotecnica Instruments S p A or your local sales representative office To obtain accessories spare parts address order or enguiry to your Biotecnica Instruments S p A sales service representative or to Biotecnica Instruments S p A and supply the following Informations a Instrument Model and Serial Number b Ouantity of parts desired c Part Number d Description Biotecnica Instruments S p A Phone 39 06 411 2316 Via Licenza 18 Fax 39 06 410 3079 00155 Rome ITALY E mail NOTE DUE TO IMPROVEMENTS IN DESIGN AND OR SPECIFICATIONS SOME PRODUCTS MAY DIFFER SLIGHTLY FROM THE PREVIOUS DESCRIPTION IMP
54. The entire electronics of the reading station excluding the photometer is integrated in two boards located on the rear of its PVC panel the Master Power Board 15 04209 02 and the Master Logic Board 15 04211 02 see Fig 6 7 6 6 Master Logic Board 15 04211 02 This board can be considered the heart of the electronic system This board accommodates the master microprocessor with a high capacity RAM and EPROM The bootstrap of the process program for all of the satellite microcontrollers is executed in this RAM This software is resident in the hard disk and can be modified if needed Another uC764 is resident on this board and together with uP552 processes an analog digital signal Observing the schematic carefully the uC552 utilizes the UART for communicating with the IBM through the 232 U7 via the RS232 The same 552 utilizes two analog inputs for reading the temperature of the cuvette plate and the vacuum level of the vacuum pump system The U8A temperature chip and U8B pressure chip amplify the analog signals of the sensors The uC764 is dedicated to the control of cuvette plate motor and the linear actuator of the washer module The U2 chip is a Programmable Array Logic PAL device programmed for generating the complex quantity of logic gates used for interfacing the photometer preamplifier with the uP552 The uC764 also uses this gate for connecting to the Master Power Board 15 04209 02 described below The U3 chip is an
55. UM PLATE CONTROLLERS 4 4 tiet reet tet ent VIII SERUM PLATE CONTROLLER MODULE 15 04422 01 VIII VACUTAINER HALL SENSOR BOARD 15 04280 01 BARCODE SCANNER tb dtt WASHING FUNNEL SAMPLING ARM WITH HEATER VIII SAMPLING ARM WITH HEATER VIII SERUM MODULE etes VIII SERUM PLATE CONTROLLER MODULE eene VIII SERUM MODULE EXPLODED VIII SERUM DEVICES arraren ertt eerta eti t kotkat VIII SERUM CONTROLLER MODULE see VIII SERUM MECHANISMS 4 inttr tte i Rd ER TRA VIII SERUM PLATE de batte eaten VIII FUNNEL CHECK VALVE SERUM CHAMBER TOP SECTION COMPUTER MODULE TITLE SECT ELECTRONICS OF COMPUTER MODULE sees IX COMPUTER MODULE oe t ee ape LE PEDE Ix MOTHERBOARD Ix ECD MODULE COMPUTER POWER SUPPLY Ix MOTHERBOARD ASSUS LAYOUT
56. ached with 4 screws to the base of the station Coat the new Peltier surface with a thin layer of thermal grease and secure it to the base of the reading station with 4 screws Tighten the mounting screws until they are snug ensuring that the pressure is applied uniformly Do not over tighten otherwise the Peltier may get damaged Ensure leads polarity is matched correctly The incorrect assembly of the Peltier can cause serious malfunction of the thermostatic system REPLACING THE HALOGEN LAMP Figure 5 5 5 4 2 Replacement of the Halogen Lamp Precautions for handling the halogen lamp a Always allow the burnt out lamp to cool down b Never touch the reflector or the lamp with bare fingers Use a rag when changing If the lamp is touched inadvertently during installation clean the lamp or reflector with alcohol and dry with a clean soft cloth before burning Contamination of the lamp or reflector may reduce lamp performance d It is recommended to initially burn the new lamp for about 30 minutes before analyzer operation Disconnect the input power before servicing The replacement of the Halogen lamp can be done by the removal of the rear panel or by sliding the transparent shutter front panel and then removing the access cover from the deck Figures 5 6 amp 5 7 SECTION V Page 8 of 16 Release the lamp assembly by gently sliding downwards Loosen the front screws in the socket and remove the burnt out lamp
57. additional function of interrupting power to the refrigeration system in case the temperature inside the reagents chamber exceeds 40 C The circuit which generates this function consists of a U6B comparator and an U7 opto coupler The Hall sensor zero position temperature sensor and the Peltier modules are all located under the reagent chamber 7 3 Reagent Refrigeration System The reagents refrigeration system comprises reagents chamber refrigeration module with the Peltier modules conductive spacers and heat sink along with the reagent pulley drive assembly SECTION VII Page 2 of 6 CONDUCTIVE SPACERS INTERFACE PELTIER LEADS REAGENTS REFRIGERATOR MODULE Figure 7 1 24 POSITION REAGENT PLATE REAGENT BOTTLES REFRIGERATION CHAMBER INSULATION REAGENT CHAMBER REAGENT PLATE Figure 7 2 Figure 7 3 REAGENT BOTTLE 20 ml 50 ml Figure 7 4 SECTION VII Page 3 of 6 REAGENT MOTOR CONNECTOR J REAGENT MOTOR WHITE BLU BLUE HITE RED ED REAGENTS DRIVE MOTOR Figure 7 5 Power Supply Reagent Temperature Peltiers Air Blower lCBUS Reagent Hall Sensor J e GP Barcode BT 2000 only Reagent Motor Reagent Controller Board 15 04313 01 REAGENTS CONTROLLER MODULE Figure 7 6
58. alibration page icon with gear The cuvette plate remains stationary as long as one is in this page 3 Enter the service page by clicking on the Check Temp page During the verification and measurements the values a 0 2 C error should be considered acceptable as per reported specifications of accuracy 12 3 Refrigeration System Calibration and Specifications The calibration of the refrigeration system requires verification and eventual calibration of the monitoring threshold of the temperature sensor The temperature sensor is located under the aluminum floor of the reagent chamber and makes a uniform contact with it to allow the refrigeration system to maintain the temperature of not less than 2 C inside reagent chamber 12 3 1 Refrigeration System Calibration Procedure Take voltage measurement of 20mV across test point TP7 and the pin 4 of the U6 on the Reagent Plate Controller Board 15 04313 01 If necessary make any adjustments through TR1 trimmer to the specified value The acceptable value is in the range of 15mV to 25mV Note On some instruments a fixed voltage resistance divider has substituted the trimmer In any case verify across the test points that the voltage corresponds to the specified voltage 12 3 2 Temperature Monitoring System Verification Test To make a real time verification of the correct operation of the temperature monitoring system observe the following procedure With your hand warm the Tes
59. alysis name Analysis Type 1st Concentration 2nd Concentration Flag 1st Result Flag 2nd Result Minimum Value Maximum Value 1st Unit of Measurement 2nd unit of measurement Unit Factor Does the 2nd result exists Method Type 9 Urine in 24 h String Character String Character String Character String String Character String Character String String String Character String String String String String String String String String String Character String Character String Identifies Male Female or Child Select one of these M Male F Female Child Identifies Routine or STAT Select one of these R Routine S STAT Transmitting patient from archive will always have identifier of Routine 9 Identifies Serum or Urine Select one of these S Serum U Urine Identifies Clinical Chemistry ISE Module or Relation Analysis Select one of these C Clinical Chemistry T ISE Module R Relation Analysis Identifies if the 2nd result exists or not Select one of these Y 2nd result exists N 2nd result does not exists If only the final result is desired then always refer to variables pertaining to 2nd result In case of the absence of 2nd result then its variables will have same values of the 151 result SECTIONIV Page 11 of 18 TABLE 1 RECEPTION The following variables are used for reception of a patient by the an
60. alyzer Variable Usage Type of valid data PATIENTCODE PATIENTNAME PATIENTSURNAME PATIENTLISTTYPE PATIENTGROUP PATIENTTYPE PATIENTURINE24H PATIENTNOTE PATIENTISCONTROL PATIENTCONTROLKNOK PATIENTCONTROLLEVEL PATIENTCLONE PATIENTCUPPOSITION PATIENTNUMBERTEST Patient Code Patient Name Patient Surname List 9 Group 2 Method Type 9 Urine in 24 h Descriptive Note If the patient is a control 4 If it is a known control Control Level 9 If itis a clone Vial Cup position Number of test Check Sum Analysis Code String String String String Character String Character String Character String String String Character String Character String Character String Character String Character String Character String Character String Identifies Routine or STAT Select only one of these Identifies Male Female or Child Select only one of these 9 Identifies Serum or Urine Select only one of these CHECKSUM ANALYSESCODE 0 Routine 1 STAT Routine 1 STAT R Routine 5 STAT ROUTINE Routine STAT STAT 0 Male 1 Female 2 Child Q Male 1 Female 2 Child Male Female Child MAN Male FEMALE Female CHILD Child 0 SERUM 1 URINE SERUM 1 URINE 5 SERUM U URINE SERUM SERUM URINE URINE SECTION IV Page 12 of 18 9 Identifies a Control or a
61. and afterwards check all the other positions observing the procedure as follows a b Start up the analyzer and let the program bootstrap begin During bootsrap press the keys lt Alt gt lt Ctrl gt and lt Shift gt simultaneously SECTION V Page 3 of 16 Let the bootstrap end a message ONLY FOR SPECIALIST appears on the screen d Align any cuvette on the cuvette plate with the washing piston and then carefully slide downwards the washer body until the piston bottoms into the cuvette e Lower the sampling arm needle into the washing funnel and press enter key Do not touch the sampling arm leave it in whatever position it is in f Enter the mechanical calibration program icon with gear symbol g Carefully perform mechanical calibration of all the positions Do not overlook any position h Perform a general reset F5 The arm needle should position itself on the center of the washing bowl Verify the correct position In case the sampling needle is not positioned correctly then recalibrate by entering the mechanical calibration program icon with gears symbol It is not necessary to turn off the instrument and perform the bootstrap process Perform a reset by clicking on the icon R and press the keys ALT CTRL and SHIFT simultaneously and then repeat preceding steps d to h 5 3 Repair or Replacement of the Reagent Module The repair or replacement of the reagent module is relatively complex because
62. ar of the PVC panel of reading station SECTION VI Page 6 of 11 6 4 2 The Washer Module System The washer module utilizes a bi directional linear stepper drive actuator which incorporates a sintered bronze nut in conjunction with a lead screw shaft to provide the washing piston carriage rapid linear movement in an upward and downward directions A photo switch is provided for reset position After the photometric measurement is complete the washing piston is lowered into the cuvette to rinse wash drain and dry under vacuum with a back and forth motion The single piston performs all three operations so that the cuvette can be used again A threaded insert made of special stainless steel is incorporated into the piston to allow for convenient piston installation and removal On the washer module the only parts subject to wear are the wash piston and stepping linear actuator Following a long period of operation the performance may begin to deteriorate If this should occurs the piston or stepping linear actuator should be checked for defects and be replaced DC power to the linear actuator is via connector located on the Master Power Board 15 04209 02 affixed to the rear of the reading station TER LOGIC BOARD 15 04211 02 MASTER POWER BOARD 15 04209 02 MNTERFACE BOARD 15 04270 01 READING STATION REAR VIEW Figure 6 7 SECTION VI Page 7 of 11 6 5 Electronics for the Reading Station System
63. as been inserted in the commands of script A string as first parameter for String or Stringn 2 Asing request command has not been inserted 3 A number request A string like parameter lt lunghezza gt of command Stringn has been inserted 04 Invalid number format Inserted invalid decimal or hexadecimal number a Inserted more than two parameters for 5 Excessivedal command String or Stringn b Inserted more than one parameter for the command Char or Set 6 Invalid data A string for command Char has been inserted 7 String Terminator Request The end 9 character of a string not found a Inserted less than two parameters for command String or Stringn E 100010 08 b No parameter rod for command Char or Set 9 Invalid String Length The string length for Stringn command is less than 0 or more than 128 a An empty string inserted for the command 10 Empty string String or Stringn b Inserted a character identified as a Tried to transfer an invalid variable in the list 11 Unknown ara Bs of internal variables YY b Tried to use a transmission variable in the script of reception or vice versa 12 Damaged file Hard disk error Contact Sales Service Internal error Probably damaged program Reinstall 13 Unknown file the program If the problem persists contact sales service a The text SET BEGIN or SET END not written 14 Incorrect identifier in the b A different valu
64. ation port and modify it manually SECTION XIII Page 10 of 10 SECTION XIV BT 1000 AND BT 2000 PLUS SPARE PARTS FOR REPAIR AND MAINTENANCE THE FOLLOWING IS LISTING OF SUBASSEMBLIES AND INDIVIDUAL PARTS WHICH ARE AVAILABLE FOR FIELD REPLACEMENT INDIVIDUAL PARTS OTHER THAN THOSE LISTED SUCH AS MECHANICAL PARTS FASTENERS ETC ARE PROVIDED ONLY AS PART OF SUBASSEMBLY THIS WIDE SELECTION OF SPARE PARTS ALLOW THE SERVICE ENGINEER TO TAILOR CONFIGURATIONS TO SPECIFIC REPAIR AND MAINTENANCE REQUIREMENTS AND TO PROVIDE REPAIR FLEXIBILITY PLEASE ASK IF YOU REQUIRE OTHER ITEMS NOT LISTED HERE AS THIS MAY BE POSSIBLE 14 1 READING STATION MODULE SPARE PARTS SECTION XIV Page I of 7 PART NO DESCRIPTION WASHING MODULE 07 04850 02 WASHING MODULE ASSY 08 04574 01 SAMPLING PINCH VALVE ASSY N C 14 2 REAGENT REFRIGERATION SYSTEM SPARE PARTS NO REFRIGERATION IN BT 1000 PART NO DESCRIPTION 03 04861 01 REAGENTS REFRIGERATION ASSY BT 2000 PLUS ONLY 06 04435 03 REAGENT CONTROLLER MODULE BT 1000 ONLY SECTION XIV Page 2 of 7 30 04522 01 REAGENT SERUM BARCODE READER ASSY BT 2000 PLUS ONLY 14 3 SERUM MODULE SPARE PARTS FLAT CABLE VACUTAINER R SECTION XIV Page 3 of 7 14 4 SAMPLER ARM SPARE PARTS 14 5 VACUUM PUMP MODULE SPARE PARTS 14 6 CONSUMABLES SECTION XIV Page 4 of 7 PART NO DESCRIPTION 662 2000 SIX MONTHLY REPLACEMENT TUBING KIT BT 1000 B
65. c circuit with a capacitive type discriminator For this characteristic the sensor can detect any type of liquid including distilled water with the same sensitivity In the normal operating conditions the minimum detectable volume is 80 ul The limiting factor is the necessity that the container is surrounded by a metallic surface The distance of the isolating material from the metal should not be more than 4 mm However in different conditions and without the surrounding metallic material around the analyzer is sensitive to minimum volumes of 100 ul 2 13 5 Contamination Errors Verification Quantify the entity of contaminations Instruments Self test and special solutions SECTION II Page 7 of 12 The elements which are potentially subject to the contamination are the hydraulic circuit of sampling and the reaction cuvettes The verification tests were performed separately to avoid reciprocal influences The verification test of residues in the reaction tests A diagnostic program integrated in the software has been utilized to fully exploit the services provided by this function 2 13 5 1 The Test with Colorimetric Method 1 An accurate washing of the cuvette No 1 5 washing cycles 2 Introduction of water into the cuvette No 1 3 Photometric zeroing 4 Emptying of cuvette No 1 5 Manual introduction of 300 ul of potassium dichromate solution 17 5 gr l equivalent to approximately 180000 ABS 6 Reading at 340 n
66. cations SECTION IX Page 5 of 7 9 1 5 Motherboard ASUS Layout Contents PS 2 Mouse Keyboard i E H i VIO USB1 USB2 LED O COM 1 T T T s gt gt 5 5 79 oO 59 E 5 5 8 amp 5 Socket 370 2 R DIP Switches 5 EIS 8 88 DIP SW 5 z B U 9 2 JTPWR 9 3 8 8 S 2 x VIA lt VT82C694X 19 C LINE USB PWRO Chipset OUT ON De lt LINE W gt IN PWR_FAN C 0 213 415 6 7 Accelerated Graphics Port gt W a ic L gt 9 6 E zs P a 8 AUX PCI 1 TUV4X 8 MIC2 mmm PCI 2 VIA VT82C686A 55 Chipset co 52 we PCI 3 USBPORT 48 ag a CR2032 3V Q Lithium Cell PCI 4 CMOS Power a S um USBPWR1 CLRTC SMB PCI 5 ASUS JEN ASIC With Hardware WOR Monitor WOL_CON CHA_FAN SECTION IX Page 6 of 7 External Connector
67. chemicals serums reagents etc 6 The safe disposal of the waste materials is the responsibility of the user 7 Insure that the disposal of rinse water is done according to all applicable laws and regulations 11 3 Technical Specifications Technical Specifications Aspiration Pump PUMP Mini Diaphragm Pump Rated Voltage res Direct Current 24 VDC Flow 9 vesen Analyzer waste Pump Diaphragm EPDM Pump Head eiiis Ryton Max Vacuum 100 mbar Liquid Level Sensor Liquid Level Float Switch with hermetically sealed magnetic Reed Switch and permanent magnet The Biotecnica Instruments S p A reserves the right to alter technical specifications without prior notice Philips Petroleum SECTION XI Page 2 of 4 11 4 Spare Parts for Repair and Maintenance The following is listing of subassemblies and individual parts which are available for field replacement PART NO DESCRIPTION 07 04856 01 VACUUM PUMP SYSTEM MODULE FIG 3 08 04716 01 H O TUBE WITH FILTER FIG 5 08 04865 01 VACUUM PUMP HARNESSED FIG 4 662 0788A WASTE PROBE FIG 1 662 0831C FEMALE FISCHER CONNECTOR KIT FIG 2 FEMALE FISCHER CONNECTOR KIT P N 662 0831C Figure 11 2 WASTE PROBE WASTE PROBE CONNECTOR FISCHER CONNECTOR OVERFLOW
68. cm Crate dimensions W 120cm D 80cm H 80cm Accessories package dimensions XXXXXXXXXXXXXXXXXXXXXX 2 3 Power Supply Requirements BT 1000 amp 2000 PLUS analyzer Universal input 90 250 Volts 50 60 Hz Max Power output 550 Watts Printer accessory Approx 50 Watts depending upon model The detailed information regarding the specifications of the instrument power supply is outlined in Section X entitled POWER SUPPLY AC DC MODULE The internal power supply of the analyzer is stabilized and incorporates the Power Factor Correction device PDF thereby require no additional external electronics accessory The use of an optional UPS device uninterrupted power supply is recommended to avoid undesired power interruptions and to ensure software integrity in case of sudden power failure SECTION II Page I of 12 2 4 EMC and Electrical Safety The BT2000 PLUS chemistry analyzer conforms to following EMC directives Electro Magnetic Compatibility EMC EN61326 EMC 55011 Electrical Safety EN61010 1 The EMC performance characteristics and electrical safety reports are available upon request 2 5 Test Execution Times for Clinical Chemistry Analyzer running cycle for clinical chemistry test BT 2000 PLUS About 17 seconds corresponding to 210 tests hour BT 1000 Less than 19 seconds corresponding to 180 tests hour The analyzer test execution velocity has been verified in the following conditions a End poi
69. correct operation of the UPS 2 The computer has not been turned on through its push button Press push button to turn on the computer 3 Hardware problem defective ASUS motherboard or power supply After verifying repair or replace After turning on the 1 The analyzer has been turned off incorrectly through program loading main switch or sudden main power line interruption bootstrap by the with consequent damaging of some files Wait for the computer is interrupted disks verification to be performed automatically by and the computer the system Verify any errors in the hard disk blocks Turning off the Contact the service engineer for any problems computer with the push 2 Hardware conflict between the system peripheral button becomes devices or damaged peripheral device The necessary peripheral device must be reconfigured or substituted in case of damage Contact service engineer 13 1 5 Trouble Shooting the Mouse and Keyboard THE MOUSE amp KEYBOARD SYMPTOMS CAUSES AND CORRECTIVE ACTIONS The system performs the boot correctly the analyzer logo appears and seems to be ready but the cordless keyboard and mouse do not work 1 2 The RF Receiver cable disconnected from the rear panel Check and correctly connect the cable Exhausted batteries in the keyboard or the mouse Substitute the batteries with the analyzer power on press the devices button to put them
70. cted back from the Vacutainer and received by the photodiode sensor element One can proceed with calibration after placing three 10mm vacutainers diameter with appropriate adapter in a triangular configuration i e in positions 02 03 29 on the serum plate There is one vacutainer sensor on the left of serum chamber which detects the presence or absence of vacutainer tubes The sensitivity threshold should be adjusted in such a way that the vacutainer sensor detects only the primary tube and not the interference from the adjacent tubes A green LED located on top of the detecting circuit provide the user with a visual indication of the sensibility threshold monitoring for distinguishing the primary tube The removal of front casing is necessary for making any adjustment or service SECTION XII Page 9 of 11 As a first step use unlabelled 10mm tubes for calibrating in worst conditions Manually rotate the serum plate with slow motion so that the three vacutainer tubes 02 03 and 29 sequentially move past the vacutainer sensor Bear in mind that the waste funnel is the reference point of the sensor located on the same axis Observe that with each vacutainer tube moving past the vacutainer sensor turns on and off the green LED turns on and off A sensor with an excessive sensibility does not distinguish the spacing between the vacutainer tubes and on the contrary it may not detect the presence of a tube particularly the one located on th
71. d wait for the outcome of transmission SECTIONIV Page 17 of 18 At the end the screen will display the outcome of the operation or show the position number of the plate where the patient has been inserted or explanation of error code sent by the instrument for example Patient Code Duplicated If the Reception procedure is selected then the program will begin initialization of communication with analyzer will ask for data of the next report ready for serial dispatch and show data of relevant downloaded report If there are no reports to be received then a relevant message will be displayed Every time the program waits for a response from the analyzer in case of problem it is possible to abort the current operation by simply pressing the Esc Escape key Note Both the programs must reside in the computer connected serially to the analyzer through appropriate cable indicated in the Operators Manual The computer must be an IBM compatible equipped with DOS operative system Windows 95 Windows 98 or Windows 2000 The operating systems such as MAC UNIX Windows ME or XP are not supported Since the programs operate in DOS ambience therefore in case the Windows operating system is used then it will be necessary to open a DOS shell the command Prompts of MS DOS is found in the menu Programs Accessories accessed through the Start button on the bottom left of the screen Both the programs use serial port with the following setups
72. difficulty slippery piston etc use lint free cloth to improve the grip Fit the new piston and finger tighten firmly by turning counterclockwise until it is properly seated against the thread shoulder Reposition the carriage into its original position Attach all WASHING PISTON tubing to the appropriate connectors WASHER MODULE Figure 5 9 SECTION Page 11 of 16 SCREW M3X25 CUVETTE P N 330 3000 J P N 02634 aN ii CUVETTE WHEEL P N 02595 O RING P N 330 5708 N ROTOR lt lt REPLACING THE CUVETTE Figure 5 10 5 4 6 Replacement of the Reading Cuvette It may become necessary to remove the cuvette wheel in order to inspect or replace damaged cuvettes Generally this operation is necessary when the following malfunctions occur a Lot of water is present on the cuvette wheel thus arousing suspicion that water may have entered the cuvette drum housing b Accidental dropping of foreign matter into the cuvettes c Accidental damage of one or more cuvettes Turn off the analyzer Push the washing piston carriage fully upward to aid in the cuvette wheel removal Unscrew the 4 screws from the cuvette wheel and carefully lift out Use a gentle oscillating movement if necessary while exercising extreme care to prevent any damage to the cuvettes Inspect the cuvette wheel rotor and housing for the presence of any liguid Clean and dry if necessary Afterwards diagnose t
73. e from ANALYSEDATA SET command transferred as lt Group name gt for the SET command 15 Damaged exit file Hard disk error Contact sales service 16 SET command not closed The SET END not inserted in the script 17 Too many SET commands More than one SET BEGIN command inserted 18 SET command not found The SET BEGIN command not found in the script 19 Incorrect variable for SET A different value from ANALYSEDATA transferred command as lt Group name gt for the SET command 20 Variable not found before The highlighted variable required in the script before the SET command the SET BEGIN command 24 The variable must be String The highlighted variable must be String type not type Char SECTIONIV Page 10 of 18 TABEL 1 TRANSMISSION The following variables are used for the transmission of a report from analyzer to the host computer Variable Usage Type of valid data PATIENTCODE Patient Code String PATIENTNAME Patient Name String PATIENTSURNAME Patient Surname String PATIENTGROUP PATIENTLISTTYPE PATIENTTYPE PATIENTNOTE PATIENTNUMBERTEST CHECKSUM ANALYSESCODE ANALYSENAME ANALYSESTYPE ANALYSESCONCENTRATION1 ANALYSESCONCENTRATION2 ANALYSESFLAGS1 ANALYSESFLAGS2 ANALYSESMINVALUE ANALYSESMAXVALUE ANALYSESUM1 ANALYSESUM2 ANALYSESUMFACTOR ANALYSES2RESULT ANALYSESSERUMTYPE ANALYSESURINE24H Group List Method Descriptive Note Number of Results Check Sum Analysis Code An
74. e heating and lasts approximately 2 000 hours SECTION VI Page20f 11 6 2 2 Reaction Cuvette Operating Principle The light is transmitted through the light cone into the reaction cuvette and then reflected into the photometer module by the quartz prism located inside the cuvette system housing The UV specific optical glass transparent cuvettes allow transmission of wavelengths down to 340nm The function of the photometer module is to monitor and measure the color variations or absorbance values between different reaction mixtures over a wavelength range from 340nm 700nm After a reaction cuvette has passed a photometer measuring position and the reaction mixture has been analyzed the reagent cuvette moves on towards the washer module where the single piston drains rinses and dries the reaction cuvette for re use 6 3 Reading Cuvette System The cuvette system assembly consists of a rotor with a circular array of 25 wells each well has a separate optical channel a cuvette plate with 25 cuvettes Figure 6 3 which fits on top of the rotor and the cuvette system housing Figure 6 4 The special optical glass cuvettes Figure 6 3 are reusable and offer excellent transmissions in the UV and visible ranges of the spectrum The underside of the cuvette system housing is fitted with 1 Peltier module relative heat sink the cooling fan and a circular heating mat The cuvette temperature is precisely maintained through the automatic te
75. e in out fluid tubing has quick connect plastic connectors to facilitate the removal of the reading station for service SECTION VI Page5ofll 4 A visible water HO reservoir with an internal liquid level sensor which is connected by a cable to the Master Power Board 15 04209 02 It also leads to the uP552 on the Master Logic Board 15 04211 02 and through photo switch U11 and Gate U10 to the reservoir feed pump located on the rear of PVC panel When the liquid level inside the water reservoir lowers the liquid level sensor activates the feed pump to refill the reservoir If the external water supply tank becomes empty after about 40 seconds the uP552 turns off the pump and the message NO WATER appears 77777722 7777 7 7777772 SZ DILUTER MODULE Figure 6 6 6 4 1 Diluter Module The diluter module is a precision liguid handling instrument incorporated into the BT 1000 PLUS and BT 2000 PLUS It uses a highly precise linear stepper motor to drive the plunger based piston inside an acrylic body fitted with inlet and outlet ports The module is mounted on the PVC front panel of reading station to facilitate service repair and allow visual verification of liguid flow The discharge and suction volumes are controlled by means of the pulse number of the stepper motor The diluter reguires minimal maintenance The diluter module is connected to the control electronics of the Master Power Board 15 04209 02 located on the re
76. e inner circle further away The sensitivity is adjusted through trimmer TR1 located on the boards 15 04278 02 and 15 04280 01 Turning the trimmer adjusting screw clockwise increases the sensitivity and turning it counterclockwise decreases the sensitivity Keep in mind that the analyzer must always function with labeled tubes therefore it is preferable to adjust calibration to lower sensitivity rather than to higher sensitivity The final test is performed by fully scanning the serum plate and by inserting at least six tubes with barcode labels Placing three tubes in triangle formation in positions 02 03 and 29 Now place three tubes in positions 15 16 and 42 Once completed go to the Patient Entry page and enter Options to start Scan Tray All At the end of scanning if the vacutainer sensor has correctly tracked the presence of all the six tubes then their barcode numbers should be visible in the patient page This means that the vacutainer sensor and the barcode scanner have functioned properly If scanning problems occur the following two types of error messages may appear a Label Not Found in Pos 28 all vacutainer tubes have been detected The barcode scanner has not read the barcode label correctly because it is defective SECTION XII Page 10 of 11 b Barcode numbers of 5 vacutainer tubes displayed instead of 6 tubes The vacutainer sensor has not detected the remaining one vacutainer tube Verify the mechan
77. e through the software During the production phase of the analyzer a special reference mask jig is used to correctly position the various modules in their locations However during maintenance and repair one must take care to operate step by step so as not to alter the original assembly Therefore it is strongly recommended not to disassemble multiple modules at one time Instead the modules should be serviced and calibrated one at a time 5 2 Repair or Replacement of the Sampling Arm Turn off all power to the instrument before replacing or repairing the sample arm Remove the casing and all of the parts necessary to access the arm subassembly see Figures 5 2 amp 5 3 Disconnect the electrical cable and the liquid heater cable Unscrew the liquid line connector from the appropriate diluter Unscrew the sampling arm assembly 3 mounting screws Gently lift the sampling arm module and place it on a safe location This operation may not seem simple but the figure here facilitates the removal Replace the repaired or substitute sampling arm module in its appropriate location observing the preceding steps in reverse order SAMPLING ARM FRONT Figure 5 2 Figure 5 3 SECTIONV Page 2 of 16 Do not tighten the sampling arm mounting screws to allow for position Manually swivel the sampling arm to verify coverage from reagent plate to the cuvette plate Also verify if the sampling needle centers the washing funnel and the reading cuv
78. ective Hall sensor or positioned incorrectly Repair or Replace Defective driver or interrupted motor Bear in mind that the arm does not make angular swivel motion until after the vertical reset To verify the correct functioning of the three arm sensors Hall sensor for UP DOWN motion Liquid Sensor Photo switch for angular motion observe the three corresponding LEDs located on a small PVC bracket near the arm shaft If the arm is moved up down manually the upper LED lights to indicate the correct functioning of the Hall sensor The touching with a finger of the arm needle will light the middle LED Swiveling the arm to the left or right will light and switch off the lower LED Repair or replace defective motor or driver The upper part of the arm needle head has loose screws and is not secured to shaft properly Dirt in the Zero position photo switch or the photo switch shutter Tighten the screws Clean with a brush the photo switch or the shutter SECTION XIII Page 5 of 10 Reset Errors Serum Plate Reagent Tray Diluter Arm SYMPTOMS CAUSES AND CORRECTIVE ACTIONS The arm needle holder does not heat up The serum plate rotates during reset and stops at an incorrect position with a screen message Reset Error The tray does not stop at the container number 1 position and issues an error message Reset Error Interrupted heater circuit inside t
79. ements Not only the user can decide to send or receive numerical information for example number of tests not as single byte but as a preset numerical string or vice versa For example the user can decide to receive something like Initiate analysis data lt Analysis data true and typical gt End analysis data Where the phrases Initiate analysis data and End analysis data do not refer to any preset data by the analyzer but serve only for monitoring communication process can be useful for inserting specific markers on those programs which obtain information from text files It is obvious that the protocol of initiation and end of communication the commands for the request of report and the analyzer responses in case of error or success remain identical to the usual preset serial communication NOTE a If a check sum is omitted in a communication then the analyzer will not control it b The following numbers have been used to represent the error codes relevant to sending a patient to the analyzer as regards the parameters not part of the standard serial communication Error Code Description Number of invalid analysis Invalid Data not through variables Invalid Data through variables An analysis variable is outside the SET BEGIN END relative to the analysis ANA SECTIONIV Page 6 of 18 Notes regarding Scripts A script is a text document Each one of the single c
80. epping motor driver or Controller Board 15 04313 01 Repair or replace SECTION XIII Page 6 of 10 13 2 Computer Module Trouble Shooting The diagnosis of computer system malfunction should be quite simple as the problem generally occurs during the powering up of the analyzer The most frequent problems are generally the loss of files or a hard disk malfunction Statistically these problems almost always are caused by incorrect operations as follows Turning off the analyzer incorrectly using back up disks that is infected with a virus incorrect use of the computer etc 13 2 1 Trouble Shooting the Computer Module COMPUTER MODULE TROUBLE SHOOTING SYMPTOMS CAUSES AND CORRECTIVE ACTIONS The analyzer starts up but no LCD display Probably a defective VGA driver or incorrect connection of the LCD display Check on the rear of display that the lamp is lit Check that the VGA board is properly inserted into the slot of mainboard Make sure that all the cables of display including the inverter cables are properly connected If necessary replace VGA Board 2 The inverter of the display lamp power supply defective or incorrectly connected Check that the inverter cable is correctly connected If necessary replace inverter NOTE It is also possible to connect an external CRT monitor to identify the problem SECTION XIII Page 7 of 10
81. ess the barcode diagnostics program The available functions allow the user to verify and optically align the scanner beam on the sample barcode labels It is also possible to read the information of the barcode label if it is present The mechanical alignment can be carried out by repeatedly clicking on the sample barcode button It is possible to precisely align the scanner beam on the barcode label after loosening the mounting screws The barcode controller can also be programmed in the diagnostics program by using a specific floppy disk available upon request SECTION XII Page 8 of 11 12 4 2 Barcode Mechanical Calibration Procedure BT 2000 PLUS ONLY In the same Mechanics page select the barcode scanner for mechanical calibration Click on the command button Laser Scan Verify that the scanner beam is precisely centered on the barcode label of the sample tube in the serum plate It is possible to correctly align the barcode module by loosening the mounting screws After the alignment fasten the screws Refer to Paragraph 12 5 entitled Vacutainer Sensor Calibration and Specifications below for the final verification of the correct operation of the barcode module 12 5 Vacutainer Sensor Calibration and Specifications The calibration consists of sensibility threshold adjustment of electro optical system LED and photodiode The optical element exploits the principle of reflection The infrared light beam emitted by LED is refle
82. ette correctly by observing the following procedure 1 2 4 NOTE Turn off power to the analyzer and unplug the power cord from the wall outlet to avoid electrical shock Manually lower the sampling needle all the way into the washing funnel to verify that it centers correctly Gently lift out needle from washing funnel and move it CCW counterclockwise to the cuvette plate Align any cuvette on the cuvette plate with the washing piston and then carefully slide downwards the washer body until the piston bottoms into the cuvette Locate and mark the position of 10th cuvette by counting anticlockwise starting from the cuvette adjacent to the cuvette with washing piston The latest production instruments have an index mark corresponding to the 10th cuvette position on the rim of the cuvette system housing for facilitating mechanical calibrations Move CCW counterclockwise and lower sampling needle into the 10th cuvette and verify that it centers correctly If the needle centers properly on the washing funnel and the cuvette then finally tighten the three setscrews on the arm head and the three mounting screws on the arm bracket If otherwise repeat preceding steps until the sampling needle is centered correctly and then securely fasten the screws and continue with the following procedure When the sampling arm is substituted or simply removed for service and then remounted it is highly important to verify the zero position
83. g arm housing The encoder s circuit is connected to the Arm Controller board 15 04765 01 Figure 8 2 in the arm housing through a flat cable The arm shaft with arm head and aspiration needle is located on the upper section of the arm housing The arm head accommodates a microprocessor based electronic circuit for liquid detection It provides infrared optical signal transmission Two infrared LEDs located underneath the arm head transmit a luminous impulse when the needle touches the liquid surface during aspiration The optical receiver is located on the upper edge of the arm controller board processes the luminous signal from the LEDs The FEP tube for transferring liquid from needle to diluter pump is pre heated at a temperature of about 40 C through a nickel chrome wire based heater SECTION VIII Page 2 of 8 The heated part is a segment of tubular heater inside the arm head The power to the sampling arm system is provided through couple of cables inside the protective sheath which connect to the reading station Angular Pos Encoder 15 04897 01 SAMPLING ARM FRONT SAMPLING ARM REAR Figure 8 1 Figure 8 2 8 2 1 Arm Controller Board 15 04765 01 The Arm Controller board 15 04765 01 secured to the arm housing Figure 8 2 is a microcontroller based control circuit connected to other modules via I2Cbus The circuit is divided into two sections of logic and power The two sections are isolated galvanically t
84. he belt Remove the damaged belt and replace it with a new belt To reassemble follow the preceding steps in reverse order Carefully slide the motor plate outwards to the left to provide the correct tension and tightness to the belt and then tighten the mounting screws 5 3 3 Replacement of Reagent Plate Drive Motor The removal operations are similar to the above paragraph 5 3 2 Replacement of the Reagent Plate Transmission Belt The motor is secured to the plate through 4 screws 5 3 4 Replacement of the Reagent temperature and Hall Sensors The removal operations are similar to the paragraph 5 3 2 Replacement of the Reagent Plate Transmission Belt Two fixing screws on one side secure the rectangular PVC housing of Hall sensor The sensor requires precise positioning to correctly couple with the magnet on the drive pulley The Hall sensor assembly must come close to the transmission belt which interposes between the magnet and the Hall sensor at a maximum distance of 1mm almost grazing the same belt The temperature sensor is housed in a rectangular metallic container which is closely secured to the bottom of the circular aluminum reagent chamber with a single screw accessible from the top of the chamber The Replacement of the temperature sensor does not require any particular care except that the two parts must be joined perfectly by properly tightening the screw No temperature verification or calibration is necessary
85. he cause of the problem Avoid removing the cuvette wheel to wash externally This should only be done if there is physical debris water spill or chemical build up in the cuvettes When replacing the cuvette wheel position the small guide hole in the cuvette wheel that coincides with the locating pin on the cuvette rotor SECTION V Page 12 of 16 Gently push the cuvette wheel downward onto the rotor Fasten the cuvette wheel securely by screwing the 4 socket head screws back onto the cuvette rotor To ensure that pressure is applied uniformly first tighten all of the screws finger tight and then tighten them in a crosswise pattern Do not use excessive force Gently push downward each cuvette until it bottoms against the depth of the rotor Attach any disconnected tubing to the appropriate ports A thorough washing procedure should be performed through the analyzer program before any testing is performed on the analyzer Note Another efficient method for cleaning the cuvettes may be used without removing the cuvettes plate from its original position Disconnect the aspiration tube located on top of the washing piston carriage and insert it into the bottom of the cuvette for few seconds to aspirate any dirt or contaminants Repeat this procedure after manually rotating the cuvette wheel to clean off the desired cuvettes The high power suction produced by the vacuum pump will quickly eliminate any dirt or contamination Reconnect the aspiration t
86. he sampling arm in this case the liquid sensor functions as the LED lights up just by the bare touch of the finger On the other hand if the LED does not light up then the electrical circuit is interrupted at the source Verify the power supply starting from the source the Master Power Board 15 04209 02 If the arm is damaged or defective then it is recommended to substitute it completely SERUM PLATE Most likely the Hall sensor malfunction The magnet on the upper rim of the serum plate is not properly aligned with the Hall sensor Defective Hall sensor or connecting circuit interrupted No rubber mat over the rotating serum base In the absence of rubber mat the magnet position is displaces a few millimeters downwards thus causing misalignment with the Hall sensor Other various causes Magnet detached from the plate or defective electronic circuit Board xxx amp Board xxxx The serum plate resets correctly but does not respect the positions and the sampling needle descends in the wrong positions Most likely the serum plate slips on the rubber mat during rotation Remove and wash the rubber mat with a neutral soap and water If the problem persists then the causes may be as follows Transmission problem with one of the following devices Timing Belt motor driver and Serum Plate Controller Board 15 04422 01 REAGENT TRAY 1 Defective Hall sensor or the sensor out of range from magnet Repair or replace 2 Defective belt drive st
87. he waste probe sensor activated warning message WASTE FULL EMPTY IT WITHIN ONE MINUTE is displayed on the analyzer screen to alert the operator In case the waste container is not emptied then another alert prompt WASTE FULL is displayed on the screen which stops pumping the liquid and simultaneously the analyzer enters the standby mode The waste pump is powered by the Pump Disable Board 15 04897 01 connector J1 The waste probe operations are controlled by the Master Power Board 15 04209 02 connector J14 The waste probe tube has a self closing quick connect for safe and rapid removal of the probe NOTE The liquid level sensors are magnetically actuated reed switch make and break type SECTION XI Page I of 4 11 2 WARNINGS 1 Do not ever leave the Waste Probe outside its external waste container during the analyzer operation or standby The waste probe must always be kept on the waste container to avoid waste liquid spillages 2 Waste products must be handled with extreme caution 3 It is recommended that disposable gloves for the safe handling of the waste in the laboratory must be worn before servicing the unit and when hands may contact potentially contaminated materials and surfaces or components 4 Carefully discard the used gloves in a proper refuse container 5 To avoid accidental contamination with chemicals pathogens and microbial contaminants use suitable guards and or personal protection when handling
88. hrough the opto isolated gates A double driver Chip 15 04408 01 with the capability of driving stepper motors is used for the motion of two stepper motors of arm The program of motors control is stored in the 1 764 which in turn receives process information from the master device uC552 that is located in the reading station The uC764 also receives digital signals from the infrared sensor encoder and from the Hall sensor for the vertical reset The I2Cbus cable connector supplies 5 Volts for digital circuits and 24 Volts for the power circuits of the arm controller 8 2 2 Arm Liquid Detector Emitter Board 15 04276 01 It is a microcontroller 764 based small electronic circuit board located inside the arm head The 8Mhz oscillator of uC764 is used for realizing amplitude modulation of signal transmitted from needle SECTION VIII Page 3 of 8 The variation of amplitude generated by the needle contacting liquid is controlled and converted by the uC764 in a chain of 40Khz impulses through infrared LED and simultaneously the red Led indicator on top of the arm head provides visual repetition of the infrared LED The circuit is powered by the 5 Volt generated by a 78L05 linear regulator which obtains the voltage from the downstream of supply line coming from the reading station No calibration is required for this circuit 8 3 The Serum Module The serum module Figures 8 3 amp 8 5 is made up of the stepper motor drive mechanisms fo
89. ical calibration of the serum plate and ultimately the sensitivity of the optical detection circuit 12 6 Stress Analyzer This program performs a limitless cycle of mechanical stress on the analyzer Generally this program is used for verifying the efficiency of replaced mechanical devices after service and repair SECTION XII Page 11 of 11 SECTION XIII TROUBLE SHOOTING 13 1 Introduction Any investigation of malfunction which might occur must begin with verification of power supply and the ambient conditions During troubleshooting the following parameters for correct analyzer operation must be verified Power Supply 90 245 Volts AC 50 60 Hz Ambient Temperature 18 32 C Relative Humidity 35 85 In case the parameters do not correspond to the indicated range of values then observe the following procedures and the appropriate corrective actions Prior to servicing the BT1000 or BT2000 PLUS chemistry analyzer switch off the instrument and disconnect the power cordset 13 1 1 Trouble Shooting Cuvette Temperature CUVETTE TEMPERATURE SYMPTOMS CAUSES AND CORRECTIVE ACTIONS Cuvettes do not 1 Ambient temperature out of limits Reenter ambient reach operating temperature limits 18 32 C temperature of 2 Defective electronic circuit causing unstable temperature or 30 32 and 37 C for temperature to exceed maximum limits Replace the or unstable temperature
90. ing system The steel base plate accommodates serum chamber with serum plate timing belt and stepping motor for serum plate rotation barcode reader needle washing funnel sampling arm electronic sensor circuit for detecting the primary tubes Hall sensor amp Positioning LED and electronic serum controller module The serum plate consists of two overlapping aluminum disks which can accommodate various types of containers capsules primary tubes of 16x100 13x75 and 13x100 with or without barcode labels There are 52 positions for samples and an additional 20 positions can accept standard and control capsules only In the latter positions the barcode reading is not provided for The tube adapters are available for accommodating different diameter tubes on the serum plate The serum plate can be placed in any position in the serum chamber as there is a magnetic device Figure 8 5 affixed on the outer rim of the serum plate for automatic positioning in combination with the Hall sensor located on the stationary serum chamber In addition there are two electronic circuits Figures 8 3 amp 8 5 installed on the outside of the serum chamber The circuit on the left is a primary tube Vacutainer reading device This circuit consists of an optical system based on reflection principle which identifies the tubes on the first and second outer rings of the serum plate circumference The Hall sensor and the red LED indicator for positioning sample
91. isolated DC DC converter which powers the photometer preamplifier It regulates 5 Volt DC input voltage into a 12 Volt DC output voltage 6 7 Master Power Board 15 04209 02 The main power board is the densest board of the analyzer It accommodates all the devices required to control the stepping motors valves etc for the reading station However for practical reasons another uC764 has been inserted to provide temperature control and regulation for the cuvette plate SECTION VI Page 8 of 11 The circuit consists of a comparator connected to the temperature sensor which is inserted into the bottom of the cuvette housing The U8 comparator output along with the U5 chip regulates the switching which generates a variable voltage in the terminals of the Peltier modules One section of the comparator controls a relay for inverting the polarity when the ambient temperature requires momentary cooling of the cuvettes system The process just outlined produces a relatively coarse temperature regulation of approximately 1 C The fine regulation of 0 1 C is performed by the software implemented in the uP552 When monitoring the temperature of the sensor the above mentioned regulator allows a continuous adjustment of the selected temperature 6 8 Interface Board 15 04270 01 The ISE sampling arm heater the cuvette motor Peltier modules of the reading station system and their cooling fans the Hall sensor and the temperature sens
92. isplayed temperature value should be identical with previous value measured with the probe If necessary adjust the TR1 trimmer on the Master Logic Board 15 04211 02 to the same value measured by the thermometer probe Make this adjustment in the shortest time possible The value of temperature measurement should not be necessarily 37 C as it is automatically corrected when exiting the service page For example the measurement of cuvette temperature by thermometer in a motionless plate equaling 35 9 C The measurement displayed on the monitor in the Check Temp function with plate rotating alternatively between clockwise and counter clockwise directions should equal 36 5 C Adjust through the TR1 trimmer until the temperature of 35 9 C is displayed on the monitor Exit service page of Check Temp After the settling time of a few minutes the monitor should display the correct temperature with fluctuation of 0 1 C The expected temperatures are the ones shown in the SETUP page of either 30 32 37 C Afterwards when the desired temperature is reached perform verification measurements with the thermometer probe in any cuvette on the stationary plate The stationary cuvettes plate mode can be selected in the following diverse manners 1 During the rotation cycle a pause occurs every 10 seconds Here the measurements must be taken quickly as the plate stops for few seconds SECTION XII Page 6 of 11 2 Enter Mechanical C
93. istant and anticorrosive Baydour plastic material to ensure the structural integrity The preceding figure illustrates the location of various modules on the analyzer Each module has a specific function for the analyzer operation 3 3 Description of Analyzer Modules The BT 1000 PLUS or BT 2000 PLUS system consists of a compact bench top analyzer with windows TM based software and is equipped with various modules and subsystems outlined below The electronic is organized in convenient modules or cards configured for simple service access and replacement 3 3 1 Computer Compartment The computer compartment consists of a LCD monitor main board power supply and peripheral devices 3 3 2 Reading Station The reading station is comprised of a cuvette plate photometer diluter washing station water reservoir and the associated electronics 3 3 3 Power Supply Module The power supply module is the general power source of the analyzer 3 3 4 Reagent System The reagent system consists of the rotating reagent plate refrigeration system barcode module BT2000 PLUS only and electronics SECTION Page 2 of 4 3 3 5 Sampling Module The sampling system consists of the rotating serum plate barcode BT2000 PLUS only Vacutainer sensors washing bowls and electronics 3 3 6 Sampling Arm Module The sampling arm module consists of an electro mechanical system incorporating electronics with appropriate Servomot
94. lect the appropriate method Normal Sample or Sample Starter In the Normal Sample mode a sequential sampling is carried out with the same needle first reagent and then serum The volume of the reagent is fixed at 300ml while the volume of the sample is selectable from 0 to 27ml Take into consideration that the instrument restores the stoicometric ratio when the volume of the sample has been selected If 3ul of serum is chosen then the reagent volume becomes 297ul After the sampling it is possible to read the absorbance values of the sample directly in the mono or bi chromatic mode The Sample Starter mode is similar to the preceding method except the sample which is dispensed subsequently by the second sampling arm The sampling test is always carried out in cuvette 1 SECTION XII Page 4 of 11 12 2 Cuvettes Calibration and Temperature Control Specifications The calibration involves adjustment of the temperature sensor to manufactures specifications A LM35 chip with tolerance of 1 C is used as the sensing element and is located inside a metallic housing Operative conditions for correct temperature calibration inside the cuvettes are as follows e Ambient Temperature 18 C to 32 C e Analyzer turned on for at least 20 minutes to reach steady state e All 25 cuvettes filled with 300ul to 6001 volume of distilled water e A certified electronic thermometer with micro probe with at least 0 1 C resolution Keep in
95. lysis can be performed quickly and with maximum reliability The automated instrument consists of the following three distinct subsystems a The Mechanical System Utilized for the transfer of the solutions samples and reagents b The Electronic System Based on microprocessors for activating drive motors alarm devices and temperature control etc c The Information Management System IBM compatible for patient inputs processing of the results and communication with the host computer 1 1 Examples of The Basic Operations of Clinical Chemistry 1 2 PHASE 1 Creation of the Worklist SECTION I Page 1 of 3 1 3 PHASE 2 Placement of Samples and Reagents 1 4 PHASE 3 Start up 1 5 PHASE 4 Process Initiation The mechanical arm is equipped with a sampling needle that can perform both swiveling and up down motions to accomplish complete mixing during the sampling cycle along with the diluter pump The cycle sequence starts with the aspiration of the reagent and the serum followed by the dispensing and mixing in the reading cuvette The sampling cycle is performed using the following devices a sampling arm a diluter pump and a peristaltic pump The sequence of the sampling cycle is as follows a b c Needle washing d Washing and drying of cuvettes Reagent aspiration Sample aspiration L Dispensing and mixing the solution in the pre washed and dried cuvette f Washing the needle and the hyd
96. m practically unnecessary because of the saturation of the photometer 7 Simple washing of the cuvette number 1 default washing with 5ml of H2O 8 Introduction of distilled water in cuvette number 1 9 Reading of the cuvette ABS 0 002 This test demonstrates the efficiency of the washing of cuvette above 1 50000 The test was repeated 10 times in the same cuvette and provided identical results 2 13 5 2 Test with Enzymatic Method The dynamic is the same as colorimetric method except that different solutions were used A concentrated solution of urea was dispensed into the cuvette at 200g l After the washing a reagent for urea was added to the same cuvette Using the same parameters of normal method the delta of absorbance was calculated so therefore the results which has been 0 0 SECTION II Page 8 of 12 The test has demonstrated the perfect efficiency of the washing system in cleaning the cuvette 2 13 6 Hydraulic Circuit Residuum Verification Only the enzymatic method was used for this test In practice using the automatic sampling the dynamic was as follows 1 Sampling in cuvette of 300 ul of urea concentrated at 200 g l 2 Simple washing 5 ml of the cuvette and simple washing 2 ml of the hydraulic circuit 3 Sampling of reagent for urea in cuvette 4 Kinetic reading in accordance with the parameters of the method 5 Result 0 5 The washing of hydraulic circuit involves Dispensati
97. m generates a FCC error if one or more cuvettes require a correction of more than 3 In this case it is recommended the FCC be repeated SECTION VI Page 11 of 11 SECTION VII REAGENT SYSTEM 7 1 General The reagent system module of the BT 1000 and BT2000 PLUS is different from the one used on the BT 3000 and BT3000 PLUS chemistry analyzers The reagent chamber reagent bottle plate and the mechanisms are of new design The Peltier module placement and their interconnection through the Refrigerator Interface Board 15 04680 02 are performed differently For effective thermal management of the refrigeration system a cross flow blower is used and through an appropriate ducting system directs the air into the large heat sink where 6 conductive blocks of Peltiers are located This cross flow blower based heat dissipation system has lower noise performance during operation than the normal propeller fans The reagents are placed in a 24 position removable plate which in turn is placed into the reagent cooling chamber to ensure maximum stability of the loaded reagents The reagents chamber is refrigerated at 4 8 C by the solid state thermoelectric Peltier modules The volume of the existing reagent in the bottle is automatically measured through the sampler arm needle The bottles can be inserted according to a reagent map displayed by the system monitor or randomly in the case of automatic recognition Large and small bottles can be used
98. m or U for Urine Send if the patient is a clone Y for Yes or N for No gt Transmit position of capsule 00 unknown Send number of tests to be executed from 01 to 99 Send codes of tests to be performed 4 characters Send 3 characters Send end transmission character EOT p Wait for response from the analyzer 2 characters If the communication is successful then the analyzer responds with character Y followed by a byte which identifies the position where patient has been inserted In case the communication was unsuccessful then the analyzer responds with N followed by a byte identifying the type of error The possible errors generated by the analyzer in response to the invalid insertion of patient are as follows SECTIONIV Page 1 of 18 0x01 Check Sum Error 0x02 Unknown Command 0x03 Routine STAT field Error 0x04 Serum Urine field Error 0x05 Clone Yes No field Error 0x06 Capsule position Error 0x07 Number of Analysis field Error 0x08 Wrong Number of Test 0x09 Position already in execution 0 0 Cloning impossible OxOB Code duplicated 0 0 One or more analysis not present the analyzer 0x0D One or more analysis not present in the current
99. mind that a thermometer probe of 2 to 3 mm diameters equipped with a K type thermocouple when immersed in the cuvette produces an instantaneous temperature drop of about 0 2 C It is recommended that thermometers of medium to large dimensions not be used to avoid incorrect measurements because of difficulty of inserting the probe and for the necessity performing measurements quickly Do not perform measurements or calibrations if the cuvettes have been filled with distilled water without waiting for essential 20 minutes warm up period 12 2 1 Cuvettes Calibration Procedure Enter the service page and select the icon Check Temperature The cuvettes plate stops temporarily Immerse the temperature measurement probe in an easily accessible cuvette Gently shake the probe with rotational movement for mixing at the bottom of the cuvette to quicken the measurement SECTION XII Page 5 of 11 After measuring and noting the correct and stable value stop the operation Do not exceed 30 seconds of measurement time of to avoid irregular heating The temperature monitoring system functions correctly only when the metallic mass of the cuvettes rotor performs in a cyclical motion Remove probe from the cuvette and click on the button which appeared previously Push Button The plate starts rotating clockwise and counter clockwise in the alternative manner instantly the temperature is displayed in real time just below the commands line The d
100. ming from the analyzer are displayed while the lower section displays the characters sent to the analyzer The only special keys used are F1 to clear the screen and F10 for exiting the program The special characters with values less than 32 are displayed in ASCII notations along with their values For example the Character EOT value 4 will be shown as EOT 4 To send a special character with values less than 32 or higher than 124 it is necessary to keep pressed the ALT key and simultaneously write the value of the character to be sent using numerical keys For example to send EOT it is necessary to keep the ALT key pressed and simultaneously enter the value 4 through the numerical key and then release the ALT key 4 9 Program BTPLUS EXE A simple communication program that simulates the host computer At the start it is necessary to identify the number of communication port from 1 to 4 and the desired procedure Transmission or Reception In case the Transmission is selected the program will ask for patient code from 1 to 15 characters the test number from 1 to 9 and the relevant analysis code for each test for example BUN It is a good practice to use the same analysis codes which the analyzer has memorized in the plate actually in use if otherwise then an error will result in the transmission phase Now the program will execute an initialization procedure of communication with the analyzer will send patient data an
101. monitoring Hall sensor and the control electronics It is divided into two distinct parts 1 The fluidic system with a washing station on the upper level 2 The photometer module and cuvette system located at the lower level SECTION VI Page I of 11 CUVETTE HOUSING HALOGEN LAMP ie PHOTOMETER MODULE Figure 6 2 6 2 Photometer Module The photometer module is mounted on the underside of the base plate of the cuvette housing The light is transmitted through the light cone into the reaction cuvette and then reflected into the photometer module by the quartz prism located inside the cuvette system housing See Figure 6 2 above 6 2 1 Light Source The photometer light source consists of a 12 Volt 35 Watt tungsten halogen lamp P N 330 9321 Figure 6 2 This light source along with the focusing dichroic reflector and the light cone keeps the light focused and centered through the cuvette This custom made UV enhanced halogen lamp provides a high radiation output in the UV and visible spectrum ranges The light cone also acts as a heat sink and attaches to the appropriate mounting surface provided on the cuvette system housing The lamp is easy to access and can be removed by gently sliding downward or upward through the lamp retaining spring clips on the light cone Once separated from the cone unscrew the 2 screws from the socket and power cable assembly to release the lamp The low wattage lamp minimizes sampl
102. mperature regulation of the cuvettes system housing by the combination of Peltier module and heating mat based heating system A bicolor LED located on the Interface Board 15 04270 01 indicates hot cold status of the Peltier modules The cuvette system housing is fitted with a temperature sensor for monitoring and controlling the cuvette temperature a Hall sensor for precise cuvette position reset and the Interface Board 15 04270 01 The Hall sensor temperature sensor 1 Peltier module 1 Peltier heat sink 1 Peltier cooling fan sampling arm heater connector and the cuvette rotor drive motor are all connected to this interface board See Figure 6 7 SECTION VI Page 3 of 11 The washer diluter module and the light cone Figure 6 2 are affixed externally to appropriate locations on the cuvette housing The drive motor Figure 6 4 is permanently mounted on a precision bracket firmly secured to the housing that provides stable support DC power is used for the electronics and the drive systems The cuvette drive stepping motor pulley turns the rotor through a timing belt Figure 6 4 and pulley based drive system The rotor has teeth on the rim and acts as the larger pulley The stepper motor turns the rotor clockwise or counterclockwise The Master Power Board 15 04209 02 drives the stepper motor see Figure 6 7 CUVETTE PLATE Figure 6 3 CUVETTE PLATE WASHING PISTON N 03 0 CUVETTE HOUSING CUVETTE ROTOR DRIVE MOTOR
103. mputer Stringn ZPatientCode 15 Char PatientType stringn ZPatientNumberTest 3 Set BeginAnalysesData Stringn AnalysesCode 04 Stringn ZAnalysesConcentration2 7 Set EndAnalysesData Stringn CheckSum 3 The details of the above scripts are as follows Stringn ZPatientCode 15 Patient Code of fixed length equal to 15 characters Char PatientType Serum type Serum Urine as single character Stringn ZPatientNumberTest 3 Number of results to be sent a string of fixed length equal to 3 characters Set BeginAnalysesData Beginning of zone repeated for the number of results to be sent see PatientNumberT est Stringn AnalysesCode 04 An analysis code of fixed length equal to 4 characters Stringn ZAnalysesConcentration2 7 Concentration referred to the analysis code as per AnalysesCode a string of fixed length equal to 7 characters Set EndAnalysesData End of zone repeated for the number of results to be sent Stringn CheckSum 3 Check Sum transferred as a string of fixed length equal to 3 characters 4 7 Serial communication test programs SECTIONIV Page 16 of 18 4 8 Program COMUNICA EXE A simple communication program for sending command characters to the analyzer and receive any response At the start the only input to the program is the number of the communication port from 1 to 4 A blue screen divided into two sections is displayed In the upper section the characters co
104. ncludes but not limited to the utilization of advancements in state of the art technology and changes thereof Liability for difficulties arising from unknown or unforeseen technical limitation is disclaimed BIOTECNICA INSTRUMENTS S p A VIA LICENZA 18 00155 ROME ITALY TEL 39 06 4112316 FAX 39 06 4103079 2003 BIOTECNICA INSTRUMENTS S p A ROME ITALY INFORMATION CONTAINED IN THIS MANUAL IS PROPRIETARY WITH BIOTECNICA INSTRUMENTS S p A REPRODUCTION OF ANY PART WHOLE MAY ONLY BE PERFORMED WITH WRITTEN PERMISSION FROM BIOTECNICA INSTRUMENTS S p A SECTION A Page I of 11 LIMITED WARRANTY BIOTECNICA INSTRUMENTS S p A warrants the BT 1000 or BT2000 PLUS analyzer system to be free from defective parts and workmanship for a period of one 1 year from the date of first purchase for use Liability under this warranty is expressly limited to repair or replacement of defective parts at the option of BIOTECNICA INSTRUMENTS S p A This warranty does not cover the results of misuse accident or abuse of any parts of the BT 1000 or BT2000 PLUS analyzer system which have been repaired tampered with or altered by anyone other than authorized personnel of BIOTECNICA INSTRUMENTS S p A Products returned to BIOTECNICA INSTRUMENTS S p A for repair or replacement shall be received prepaid If found not to be defective under the terms of warranty a charge will be made for repair or replacement and freight costs will be at
105. nds the analyzer sends the requested report if available or the character NAK 0x15 if there is no report to be sent It must be borne in mind that after a run test the reports are not immediately available for transmission as these need validation To do this go to Utility menu RS232 and enable the option Accept result to be sent This operation must always be performed after each run test or groups of run test There is also an additional option for performing validation operation automatically Go to Setup of the analyzer Menu Utility Setup Analyzer go to the Serial fourth from the left and enable the option All results must be sent automatically without validate at the bottom of the page In case of positive response to the request for a report the analyzer transmits Patient code 15 characters List type for Routine or R for STATS Sample S for Serum or U for Urine Number of reports 3 characters For each report Analysis code 4 characters Result 7 characters Check Sum 3 characters lt EOT gt SECTION IV Page 3 of 18 The following is an example of eventual response to the data sent in Sending a patient to BT 1000 BT2000 PLUS 000000000000001RS003GLI 000 000BUN 0010 10COL 00100 0107 lt EOT gt Where 000000000000001 Patient code dL Identifies STATS list
106. neously to the previous test using the same dynamics for times It took 14 minutes to reach 37 C Ambient conditions consisted of 20 C temperature an RH of 33 and the initial temperature in the cuvette of 22 C 2 7 Volume and Consumption for Washing The specifications below indicate the volumes necessary for test and washing Volume in cuvette 290yl min 700 max Volume of to wash cuvette 5 0ml 10 Volume of to wash needle 2 0 10 Volume of H2O for shut down wash 140 ml 10 Volume of solution for Extra Wash 140 ml HO 13ml Detergent 10 Volume of 2 for Zeroing 140 ml 10 SECTION II Page 3 of 12 2 7 1 Residual Volumes in Reagent Containers Bottle 50 ml 1 0ml Bottle 20 ml 0 5ml Bottle 10 ml 0 5ml 2 8 Cuvette Temperature Control The cuvette plate is thermally controlled in a very precise way The solution dispensed in the cuvette reaches the working temperature in minimal time possible However it is possible to observe a notable time difference due to the potential difference of the temperature of reagents Therefore the heated sampling tube aids in preheating the reagent before dispensing The stabilization times of the solution in cuvette range from 90 seconds to 60 seconds with reagent temperatures at 11 C and 22 C respectively The time was measured after the solution had reached 36 7 C Accuracy measured after 2 minutes 0 25 Adjustment measured after 5 minutes
107. ng and quality control equipment It is staffed with highly experienced personnel with a dedication to quality The company supplies more than just products it provides highly efficient technical and manufacturing support based on the requirements of its customers with ongoing training programs to keep them current on advancements in technology A global network of distributors and sales engineers assure the most comprehensive customer support across the world For more information about Biotecnica visit the Biotecnica web site at Please do not hesitate to contact us We will be pleased to assist with any inquiries you may have SECTION A Page 8 of 11 PACKING UNPACKING THE ANALYZER UNPACKING THE ANALYZER amp ACCESSORIES Two wooden crates contain the analyzer and the accessories The crates can be easily opened by applying a lever action with a large screwdriver to remove all the spring clips on the base of the crate as shown in the figure below Carefully remove the upper covering from the base Remove the analyzer and place it ona stable vibration free surface level table or cart Carefully unpack all the accessories and place them in a protected place Store the empty wooden crate assy in a safe place for future use Arrow Pointing Upwards Base Spring Clip SECTION A Page 9 of 11 Verify upon receipt of the BT 1000 or BT2000 PLUS analyzer system that all parts are present and intact when opening the
108. ng piston If the device makes no movement then there is a defective circuit of the power driver or a defective motor Consideration should be given to the fact that all modules have the same basic structure motor driver position sensor and the micro controller The service operations for resolving the problem are deliberately confined to the substitution of the defective devices already mentioned In case the service engineer is unable to resolve the problem the whole electronic circuit of the module can be substituted It is important to verify that the devices are properly connected and that the cables and connectors are free of defects Outlined below are some examples of electromechanical problems with appropriate messages of Reset Error XXX SECTION XIII Page 4 of 10 Reset Errors Serum Plate Reagent Tray Diluter Arm SYMPTOMS CAUSES AND CORRECTIVE ACTIONS Diluter blocked Diluter functions but does not reset Sampling arm moves vertically without resetting Sampling Arm blocked Zero position calibration of the sampling arm not maintained in successive resets DILUTER Defective motor driver micro controller connecting cable or PCB Repair or replace Defective photo switch micro controller connecting cables or PCB Repair or replace In case the Diluter is damaged or defective then it should be completely substituted SAMPLING ARM Def
109. nt method incubation time 8 minutes for BT 1000 b End point method incubation time 7 minutes for BT 2000 PLUS Reagent volume 300ul with filling to 80 of appropriate bottle d Note that the time needed for test incubation and the creation of a report is not Sample volume 3ul taken into consideration 2 5 1 Non Test Execution Times Other functions of the analyzer are noted below with their appropriate time required Washing amp filling time of cuvette 6 minutes Ordinary washing time 5 minutes Zeroing time 8 minutes FCC execution time 17 minutes SECTION II Page 2 of 12 2 6 Analyzer Warm up Requirement The analyzer takes about 20 minutes to warm up for optimal operation The verification of warm up and stabilization has been specifically performed on the Halogen lamp photometer and cuvette temperature controller 2 6 1 Analyzer Photometer The maximum stability test of the photometer has been performed using a diagnostic program where a graphic curve of absorbance versus time was traced First the distilled water was introduced into 25 cuvettes on the reading plate Every 2 minutes a bichromatic reading of absorbance 340nm 700nm was performed until reaching the stable value The successive readings after the last value to be considered stable showed a maximum drift of 4 points in the first hour and less than 2 points in the successive hours 2 6 2 Cuvette Temperature This test was performed simulta
110. of the necessity to remove many parts before the extraction of whole module It is important to turn off power to the analyzer before any repair or replacement After the repair or replacement no mechanical re calibration is necessary However there is the possibility that the sample arm may reguire an angular position adjustment through software program The following situations may reguire service personnel intervention 1 Replacement of one or more Peltiers 2 Replacement of the transmission belt 3 Replacement of the drive motor 4 Replacement of the temperature or Hall sensors SECTIONV Page 4 of 16 For these operations refer to Section VII entitled Reagent System and associated photos 5 3 1 Replacement of Reagent Peltier Modules This operation requires the removal of the front cover Refer to the photo sequence Section VII entitled Reagent System Disconnect all of the connecting cables unscrew the 4 mounting screws securing the Peltier module and remove the module Turn it upside down and carefully place it on a surface Unscrew and remove the two heat sinks Now observe the exposed group of Peltiers and the electrical connections soldered to a PCB Check for the defective or interrupted Peltier using the Ohmmeter Bear in mind the difficulty of instrumental measurement because of the emission of residual energy produced from the Peltier The use of digital instruments may provide a faulty measurement It is recommended
111. off suddenly without any apparent reason The vacuum pump system makes unusual noises 2 UPS if installed generates continuous alarms 3 The analyzer generates random resets of mechanical devices or the software 1 2 3 Power supply problems defective main power power cord not properly connected UPS connected incorrectly Get your main power line checked and repaired by a competent electrician Connect power cord correctly Main power line generating high surge voltage due to lack of ground connection Request your electric company to verify and repair the line if necessary Defective UPS or discharged batteries Disconnect UPS temporarily to verify if it is causing the problem If necessary substitute with new UPS Try to recharge the batteries or substitute them Bear in mind that the UPS supplied with the instrument is universal type and it cannot be used in extremely critical situations where the electrical lines are subject to large surges In such cases it is recommended to use a more sophisticated UPS i e NOBREAK etc SECTION XIII Page 2 of 10 13 1 4 Trouble Shooting Power Up Problems POWER UP PROBLEMS SYMPTOMS CAUSES AND CORRECTIVE ACTIONS After turning on the 1 No power due to various causes The monitor is off analyzer nothing only the refrigerator fan can be heard Verify all the happens connections and the
112. ommands must each reside in a different line and be complete In other words a single command cannot be divided into more lines Stringn Name 10 gt Valid line Stringn Name 10 char gt Invalid line Stringn Name gt Invalid Command 510 An editor for writing modifications saving and compiling of one or more scripts is accessible inside the program setup function In any case it is possible to write a script with any text editor DOS or Windows like Notepad of Windows or the EDITOR of the DOS It is not possible to import documents written with UNIX as the characters used for going to the next line are different from the ones used by the DOS or Windows CAUTION If one wants to use the script stored in a removable disk for example floppy disk then it will be necessary to copy it on the hard disk TYPE OF DATA Character Identifies a single character can pass as printable character enclosed between single apostrophes as decimal ASCII value followed by symbol or else hexadecimal ASCII value followed by Ox If for example we want to identify the character A decimal value 65 or hexadecimal value 41 then we can write A 65 or 0x41 String Identifies a sequence of printable characters enclosed in single apostrophes for example this is a string Comment Identifies a portion of test preceded by a character which will not be compiled but will serve as note only for the programmer Va
113. on of 2 ml of water in the circuit starting from peristaltic pump passing through diluter and out of the sampling needle The needle is further washed externally with 100 and 200 ul of water when inside the washing funnel The efficiency of washing of the hydraulic circuit is fully demonstrated by the resulting value of 0 5 ul after having contaminated a cuvette with a solution concentrated at 1000 times Result The test has demonstrated the efficiency of the washing system of the hydraulic circuit 2 13 7 Sample Viscosity 340 700 nm This test was performed for evaluating the variation of absorbance due to the use of viscous solutions in sampling compared to the absorbance of the same solutions without sampling The potassium dichromate solution 1 75 gr l with addition of PEG 6000 100 gr l PEG 1 and 50 gr l PEG 0 5 was used as sample for viscosity test The sulphuric acid 0 01N was used as reagent The PEG at concentration of 100 gr l gives the solution a viscosity twice as much that of a serum SECTION II Page 9 of 12 Starting from the concentrated solutions the reference solutions samples of 3 10 and 30 were manually prepared for a direct reading on the photometer for verification of initial absorbances without sampling These reference samples were prepared with 300 times larger volumes with respect to the samples read by the analyzer to minimize any manual error The readings were repeated 15 times and the resulting values
114. onforms also to Italian Regulations CEI EN 61010 01 and CEI EN 61326 1 EMC The conformity is attested when the eguipment is installed in accordance with the conditions outlined in the manual SECTION A Page 7 of 11 THE COMPANY PROFILE Biotecnica was founded in 1972 with years of technical expertise and manufacturing knowledge in the field of design production sales and technical support behind its staff The Company develops and manufactures clinical chemistry equipment and relevant reagent products to be sold directly and through OEM agreements The market consists of private and state clinical laboratories hospitals clinics day hospitals and universities etc Since that time the company has developed and produced instruments of uncompromising performance characteristics which has enabled Biotecnica to become one of Europe s leading manufacturers of clinical chemistry instruments Biotecnica is headquartered in Rome Italy and markets products both domestically and internationally through a network of distributors Our actual range of instruments is composed of e Photometers for clinical chemistry e Automatic analyzers for clinical chemistry e Electrophoresis densitometers amp accessories e Flame photometers amp associated reagents e lon selective analyzer e Clinical chemistry reagents Biotecnica has over 3 000 square meters of manufacturing facility which houses the latest in engineering manufacturing testi
115. or and sampling needle Note For detailed description of the preceding modules refer to Sections V to XII of this manual 3 4 System Electronics The electronics of the analyzer with the exception of the IBM compatible computer is composed of 80C51 micro controllers All the modules are defined as intelligent peripherals Micro controller 87LPC764 is programmed to perform process control functions assigned to it as a slave driver The master functions are performed by a 552 microprocessor which communicates with the main board of the IBM through RS 232 serial port The modules communicate between them through the protocol established by only two conductors SCL and SDATA The wiring of the instrument is extremely simplified with the cable connecting various modules has only two wires for communication and additional wires for power supplies The power supply of the instrument is composed of two power supplies of high quality One power supply is located inside the computer module and is used for powering the IBM module with its various peripheral devices The 5 Volt power supply in addition to supplying the main board is used for energizing the series of micro controller modules SECTION III Page 3 of 4 The second power supply located outside the computer module is for the supply of voltage to the power circuits The three voltage outputs power the following systems 1 24V 8A variable from 18 to 26 Volts f
116. or are all connected to the interface board A bi color LED indicator is also located on this board to visualize the hot cold status Note For inspection or service and maintenance it is easy to access the master logic board master power board photometer diluter pump peristaltic pump or any other device of the reading station system by simply removing the corresponding small panel on the rear of the analyzer The integrated electronics make the module an autonomous intelligent peripheral after having received the information from the central processing unit IBM 6 9 10 Channel Photometer The Biotecnica 10 channel photometer is a discrete device and has ten channels with interference filters Each channel has an independent filter photodiode and amplifier The unigue optical system provides for simultaneous illumination of all of the channels The electronics circuit is capable of communicating and acquiring signals from 2 channels for bi chromatic reading The 10 amplifiers and as many diodes are visible on the schematic Every amplifier is eguipped with a trimmer for gain regulation The gain adjustment is necessary for adapting the signal level to the manufacturing tolerances of optical components SECTION VI Page 9 of 11 The two multiplexers U6 and U7 and their outputs that are connected to the inputs of two A D converters U8 and U9 establish the simultaneous selection of two channels The conversion is serial type at
117. or Keyboard amp Mouse 1 Serial Port O for optional peripheral equipment 1 VGA Connector for external monitor SECTION Page 14 of 16 The ASUS main board with a 1 GHZ Intel Pentium III processor 128 Mb RAM and the peripheral boards are all affixed inside the Computer Module 7 The 10 Gb Hard Disk is secured under the computer module The computer Power Supply O is located above the Computer Module over the LCD display panel The analyzer Power Supply 40 Floppy Disk Drive amp the DVD CD ROM Player behind the power inlet panel item 11 are located to the left of Computer Module Product enhancement resulting from new innovations in technology and from our continuing quality improvement effort may necessitate changes in this configuration in the future However any eventual changes will be fully compatible with the previous version A connecting cable composed of lead conductors of the serial port and the 5 Volt supply interconnects the Computer Module and the process electronics 5 5 1 Repair and Replacement of the Computer Module Hardware The computer module is located on the rear right of the analyzer see item 7 Figure 5 12 The motherboard and the peripheral boards etc are affixed inside the module which can be slide opened Inside the lower opening below the module one can observe the hard disk DVD CD ROM player and the floppy disk drive are located behind the power inlet panel item 11 Figure 5 12 to the left of
118. or refrigerated Peltier groups 2 24V 8A stabilized for the servomotors solenoid valves pumps temperature controls and fans 3 12V 8A stabilized for Halogen lamp of photometer All of the supplies are isolated to enhance digital communication through the opto electronic devices thus greatly reducing the interference which is typical of direct digital power circuits communication Both of the power supplies incorporate PFC and have a universal input which ranges from 90 to 250 Volts 3 5 Example of the Configuration The I C BUS uses several microprocessors as shown below Microprocessor Microprocessor Microprocessor Microprocessor Master Logic Slave Logic Slave Logic Slave Logic Peripheral Peripheral Peripheral SECTION Page 4 of 4 SECTION IV SOFTWARE SERIAL COMMUNICATION OF THE BT1000 amp BT 2000 PLUS HOST COMPUTER 4 1 General The analyzer BT 1000 2000 PLUS allows bidirectional communication with any host computer connected serial to the analyzer There are primarily 2 communication protocols Standard and Variable 4 2 Standard Protocol 4 2 1 Patient Transmission Start communication with sequence STX lt gt ACK gt Send patient 15 characters gt Send list type for patient insertion T for Routine or R for STAT gt Send type of 5 S for Seru
119. perating conditions for other samples For each weight measurement the four numerals after the decimal point were considered significant SECTION II Page 11 of 12 Results ml Sample A Total Evaporation in 4 pl Evaporated Evaporation hours 6 PEG Tube 0 0149 14 9 ul 0 248 6 Acid Tube 0 0158 15 8 ul 0 263 6 Serum Tube 0 0226 22 6 yl 0 376 05 Capsule 0 0447 44 7 8 94 05 Acid Capsule 0 0442 44 2 8 84 0 5 Serum Capsule 0 0464 46 4 9 28 After one hour of work average duration of the samples on the plate the following percentage of evaporation occurs ml Sample A 15 hour Evaporated Evaporation 6 PEG Tube 0 004 4 ul 0 067 6 Acid Tube 0 0048 4 8 ul 0 08 6 Serum Tube 0 005 5 ul 0 083 0 5 PEG Capsule 0 0136 13 6 ul 2 72 0 5 Acid Capsule 0 0132 13 2 ul 2 64 0 5 Serum Capsule 0 0138 13 8 ul 2 76 SECTION II Page 12 of 12 SECTION Ill ELECTRONICS Top View of the BT 1000 PLUS amp BT 2000 PLUS 3 1 uuy anpoy ejejq Wnies O einpojy juebeay 2 Ajddng O uogejs snoueA Only on BT 2000 PLUS AALNANOD N VON lt lt 5 7 uogejs Buipees amod SECTION Page 10 4 3 2 Analyzer Structure The analyzer outer casing and covers are molded in durable impact res
120. r serum plate movement electronics Vacutainer sensor with electronics and the Serum Plate Controller Board 15 04422 01 The serum mat Figure 8 11 at the bottom of serum chamber provides anti skid surface for the serum plate stability during rotation It should be cleaned at least once a month with neutral detergent only Do not use alcohol or any solvents to clean the mat In case of any liguid spills clean the serum mat immediately The new design of the serum module Figure 8 3 amp 8 5 integrates the entire sampling system into a single module with the following features a Autonomous testing of the module before incorporating into the analyzer b Compact hydraulic circuit providing stabilized flow efficiency c Hydraulic circuit hidden from view for safety Technical assistance facilitated by the ability to substitute the whole module with other tested sampling module 8 3 1 Serum Plate Controllers The serum plate controllers are a group of various electronic circuits applied to the serum module The main controller circuit is housed in a metallic container Figure 8 4 8 5 amp 8 7 located near the serum chamber The additional circuits are firmly secured to the rim of the serum chamber These electronic circuits perform the following functions a Control of the serum plate stepper motor b c Control of the Hall sensor for home or zero position d Acguisition and management of the barcode data of the serum plate Control
121. raulic circuit The diluter provides the aspiration of the solution A built in liquid sensor attached directly to the needle mechanism controls the descent of the needle into the reagent or sample This controlled immersion of the needle avoids any excessive contamination The first part of the hydraulic circuit located inside the sampling arm is the pre heating of reagents to about 40 C in order to compensate for the low temperature of the reagent The peristaltic pump provides about 2 0 of water into the waste bowl to wash the needle and the attached hydraulic circuit SECTION I Page 2 of 3 1 6 PHASE 5 Reading Cycle In this phase the sample remains in its cuvette for the programmed incubation time after which the reading phase of one or all the cuvettes will begin The reading cycle takes place every 18 seconds and lasts about 2 seconds The photometer located under the reading station converts the luminous intensity of the cuvettes into digital signals and transfers them to the microcontroller After the initial reading the acquired data is stored as concentration and absorbance values for generating the test results SECTION I Page 3 of 3 SECTION Il TECHNICAL SPECIFICATIONS FOR BT 1000 2000 PLUS 2 1 Ambient Conditions of Testing 2 2 Physical Dimensions of the BT 2000 PLUS Analyzer without accessories 66 Kg Packed instrument Kg xxx Accessories Kg xxx Analyzer dimensions W 78cm D 58cm H 70
122. rboard Two wires of the display module are connected to the LCD Inverter From the LCD Inverter other wires are connected to the LCD Interface as shown in the schematic below 5 2 gren MOTHER BOARD ASSY 07 04487 01 C 12 0077 01 E LCD Module Interconnection Schematic 15 04845 01 SECTION IX Page40f7 Mechanical Specifications of LCD Display PARAMETER SPECIFICATIONS UNIT Display Size 31 12 1 Diagonal cm Active area 246 H x 184 5 V mm 800 H x 600 V pixel Pixel format 1 pixel R G B dots Pixel pitch 0 3075 H x 0 3075 V mm Pixel configuration R G B vertical stripe Display mode Normally white Unit outline dimensions 290 0 W x 225 0 H x 13 2 D mm Mass 830 30 g Surface treatment Anti glare and hard coating 3H Haze Value 28 9 1 4 COMPUTER POWER SUPPLY MODULE Input Voltage Range Electrical Specifications PARAMETER MIN NOM MAX Unit V input Range 100 115 230 240 V rms Output Voltage Range Electrical Specifications Output Nominal Regulation Ripple amp Noise Output Current Peak Voltage Value Load Min Max 1 3 3V 5 50mV 0 3A 14 2 5V 5 50mV 2A 20A 3 12V 5 120mV 02 6 16 0A 4 5V 5 100mV OA 0 3A 5 12V 5 120mV OA 0 8A 6 5Vsb 5 100mV OA 2A Note The Connector meets AT standard specifi
123. riables These are particular sequence of characters preceded by the symbol which will be used by the program for storing internal informations patient code analysis name and etc refer to TABLE 1 TRANSMISSION RECEPTION There are also variables for direct uses which allow for identification of any character below ASCII 32 space to facilitate the writing of the script for example one can use the variable EOT to identify the character 4 see TABLE 2 INTERNAL VARIABLES SECTIONIV Page 7 of 18 SCRIPT FUNCTIONS String Identifies a string of variable length ending with a particular character Syntax String lt string gt I lt Terminator gt Where lt String gt Transmit receive string lt Terminator gt End character Note It is not possible to use the variables like parameter lt Terminator gt Example String Hello Word 0 String My String Q String Variable1 0x10 Stringn Identifies a string of fixed length Syntax Stringn lt String gt lt Length gt Where lt String gt Transmit receive string lt Length gt String length Note If the length of the text strings is less than the data length then a series of spaces will be added on the right to reach the data preset length In case the text string is longer than the data length then the string end will be cut off to match the data length If the length of the numerical values is less than the data length then a series of characters
124. rogressively to about 2 200 points of optical density e Beyond 28 000 towards 30 000 points the amplifiers tend to saturate and because nonlinearly at lower values of optical density SECTION XII Page 2 of 11 12 1 2 Photometer Calibration Procedure Select a pair of sequential filters using diagnostic program i e 340nm 380nm Execute a reading of the 25 cuvette plate and observe the values of integration If necessary adjust the trimmer corresponding to each channel clockwise to increase and counterclockwise to decrease the measured values At each adjustment perform reading of the 25 cuvette plate for verification Select the next pair of interference filters i e 405 436nm and repeat the preceding operations Continue this until terminating with the last pair of 630nm 700nm filters The calibrations can also be performed in the random mode 12 1 3 Verification of Any Problems During the Reading The verification of the photometer requires the following selection of filters 1 and 2 the filling of one or more cuvettes the gain reading of the amplifier of desired channel the reading of offsets of all the channels the reading of the absorbance in mono and bi chromatic modes zeroing the absorbance of all the cuvettes sample one cuvette using the reagent number 1 and serums number 1 and then read its absorbance value 1 Click on the Select Filter and select the 2 filters 2 Click on the Read zero filter to have the
125. s 1 PS2KBMS PS 2 Mouse Port Connector 6 pin female 2 PS2KBMS PS 2 Keyboard Port Connector 6 pin female 3 USB Universal Serial Bus Connectors 1 amp 2 two 4 pin female 4 PRINTER Parallel Port Connector 25 pin female 5 COM1 COM2 Serial Port Connectors two 9 pin 10 1 pin male 6 GAME AUDIO Game MIDI Connector 15 pin female 7 AUDIO Audio Connectors three 1 8 jacks Internal Connectors 1 IDELED IDE Activity LED 2 pin 2 FLOPPY Floppy Disk Drive Port Connector 34 1 pin 3 IDE IDE Connectors Two 40 1 pin 4 WOL CON Wake On LAN Connector 3 pin 5 WOR Wake ON Ring Connector 2 pin 6 CPU PWR CHA FAN Chassis and CPU Fan Connectors 3 pin 7 CHASSIS Chassis Intrusion Lead 4 1 pin 8 SMB SM Bus Connector 5 1 pin 9 JIR Infrared Module Connector 5 pin 10 USBPORT USB Header 10 1 pin 11 ATXPWR ATX Power Supply Connector 20 pin 12 AFPANEL ASUS Panel Connector 12 1 pin 13 MIC2 Internal Microphone Connector 3 14 CD AUX MODEM Internal Audio Connectors three 3 pin 15 PWR LED PANEL System Power LED Lead 3 pin 16 SPEAKER PANEL System Warning Speaker Connector 4 pin 17 MSG LED PANEL System Message LED 2 pin 18 SMI PANEL System Management Interrupt Lead 2 pin 19 PWR SW PANEL ATX Soft Off Switch Lead 2 pin 20 RESET PANEL Reset Switch Lead 2 pins SECTION
126. t Zone of the reagent chamber the Test Zone corresponds to the position of the temperature sensor SECTION XII Page 7 of 11 In these conditions the Peltiers group will be powered by the maximum current and by the maximum available voltage of about 28 volts In addition the maximum available voltage of about 26 volts will power the cross flow blower Take voltage measurement of Peltiers across the test point TP6 and the pin 4 of U6 Cool off the Test Zone of the reagent chamber with ice In these conditions the supply voltage for the Peltier and the cross blower will diminish sharply until reaching the value of about 18 volts Measure the voltage of Peltiers across the test point TP6 and the Pin 4 of the U6 After the instrument has reached steady state with ambient temperature of 26 C maximum the temperature measurement taken near the Test Zone should be between 2 and 7 C The measurement should be taken with a contact thermometer 12 4 Barcode Calibration and Specifications BT2000 PLUS ONLY The calibration involves the aligning of the barcode scanner beam in the precise direction corresponding to the sample tube in the serum plate The mechanical adjustment should be performed with the aid of the diagnostic program The barcode module should also be programmed for the special reading of up to five types of barcode labels 12 4 1 Programming of Serum amp Reagent Barcode BT 2000 PLUS only Click on Mechanics button to acc
127. transformation in script of the standard routine of the patient reception by the analyzer Stringn ZPatientCode 15 Char HPatientListType Char HPatientType Char HPatientClone Stringn PatientCupPosition 2 Stringn ZPatientNumberTest 2 Set BeginAnalysesData Stringn AnalysesCode 4 Set EndAnalysesData Stringn CheckSum 3 The following are the details of the above Scripts Stringn PatientCode 15 Patient Code of fixed length equal to 15 characters Char PatientListT ype Type of list Routine STAT as single character Char PatientType Serum type Serum Urine as single character Char PatientClone Identifies if the patient is or is not a clone single character Stringn ZPatientCupPosition 2 Position of serum cup string of fixed length equal to 2 characters Stringn ZPatientNumberTest 2 Number of tests to be executed string of fixed length equal to 2 characters Set BeginAnalysesData Beginning of analysis codes Stringn ZAnalysesCode 4 An analysis code of fixed length equal to 4 characters It must be entered for each type of test as per qty indicated in the ZPatientNumberTest Set EndAnalysesData End of analysis codes Stringn CheckSum 3 Check Sum transferred as a string of fixed length equal to 3 characters SECTIONIV Page 15 of 18 The following examples are the transformation in script of the standard routine for the transmission of a report by the analyzer to the host co
128. transmission values in the dark of channel 1 amp channel 2 amplifiers The values should be between 0 and 390 Values higher than that may cause problems in the photometer electronics or produce an incorrect mechanical calibration of cuvette tray i e light passage in the cuvette during reading in the dark 3 Fill all the cuvettes with water by first clicking the Cuvette button and then the Wash and fill up button It is possible to perform additional controls after the cuvettes have been filled 4 It is possible to read the absorbance and the relevant values of the amplifiers on all the cuvettes by simply clicking the Read button The sequence of the values has the following meaning SECTION XII Page 3 of 11 Cuvette 1 Chan 2 chan mAbsx1 mAbsx2 H cuv rel gain rel gain abs filter 1 abs F1 F2 Es 1 18 000 18 900 300 100 Selected Filters 340nm 630nm The above example shows that the cuvette 1 with water has an absorbance of 300 mABS at 340nm and an absorbance of 100 mABS in bi chromatic reading at 340 630nm The relative gains of the channels 1 and 2 should have the values between 14 000 and 28 000 when all the cuvettes are filled up with distilled water If the zeroing of the absorbance is desired then click on Zeroing button if necessary click a couple of times When performing the a sample and read process using reagent in position 1 and sample in position 1 click on the Sampling button and se
129. tube are connected to this circuit through a flat cable SECTION VIII Page 1 of 8 The second electronic circuit located on the right of serum chamber is utilized simply as interface connection to the control electronic module of the sampling system This serum controller module is installed on the right side of the base plate as illustrated in Figures 8 3 8 4 8 5 and 8 7 8 2 Sampling Arm fig 8 1 8 2 This is a mechanical system dedicated largely to the movement of the sampling needle Two belts driven pulleys connected to two stepper motor shafts provide vertical and angular motion As regards the vertical movement the reset position is obtained through a magnetic system the Hall sensor on stationary part and magnet on the mobile part For angular movement a high tec absolute encoder is used These are two Hall effect microchips one chip is positioned on the stepper motor shaft and the other on the reducer pulley The first chip performs high resolution reading of the angular position of the motor shaft and the second chip performs a low resolution reading of the physical position of the sampling arm The combined readings of angles by these chips provide precise placement of needle on the preset positions thus assuring correct descent of needle even if it was displaced inadvertently The encoder sensors are installed on the printed circuit board 15 04767 01 Angular Position Encoder Figure 8 2 affixed to the bottom of samplin
130. ube after removing dirt or contaminants Caution It is required that the SET LAMP procedure be performed after replacement of one or more cuvettes If the removal and replacement of cuvette wheel required no replacement of cuvettes the same SET LAMP procedure is required CUVETTE HOUSING HALOGEN LAMP PHOTOMETER MODULE PHOTOMETER MODULE Figure 5 11 5 4 7 Photometer Replacement The box containing the photometer module is mounted on the underside of the base plate of the reading station Turn off the power to the analyzer before replacing the photometer The photometer module can be easily removed by unscrewing the 4 mounting screws on the base plate Caution It is required that the FCC procedure be performed after replacement of the photometer SECTION Page 13 of 16 Rear View BT 2000 PLUS Computer Module Electronics FIGURE 5 12 5 5 Introduction to the Computer Module Hardware The LCD Display module is on the front left of the analyzer The peripherals DVD CD ROM player and the floppy disk drive are located to the right of the display An on off switch for the analyzer is located below the floppy disk drive All of the connections to the external peripheral devices are located on the rear of the LCD Display in the Computer Module as follows 1 Parallel Port 1 for the Printer 1 Serial Port for Host Computer 1 Serial Port for UPS 3 USB Ports for optional peripheral equipment 2 PS 2 ports f
131. whether it is performed with the photometer test box or directly on the analyzer A quality control report from the photometer may present different amplification values after it has been installed on the analyzer This is due to the sum tolerances of the entire optical system halogen lamp and quartz prism light guide However any slight deviation is tolerated if the values are within the specified limits Optical conditions for calibrations and reading of values 1 Reading station fully tested and electro mechanically functional 2 Cuvettes calibrated and properly cleaned as per specifications Distilled water in all the 25 cuvettes 300 to 6001 4 Lamps tested as laid down in the specifications see specification label Warm up period of at least 5 minutes to allow the system to reach steady state Specifications are valid for the BT 1000 2000 PLUS with a reading cycle of 1 5 seconds and 25 cuvette plate The average value of signal for all the channels is 24 000 points recommended value for calibration Limit values of signals for all the channels are from 14 000 to 28 000 points Note An average value of 24 000 points is recommended as the halogen lamp progressively burns out exhausts After about 1 500 hours of burning the lamp may reach a minimum acceptable value limit of 14 000 points The acceptable minimum and maximum values have the following implications e Below 14 000 points the photometric resolution tends to reduce p
132. wooden crates and packaging If there is a damage or missing items then please fill out the form found in the Operator s Manual Refer to Section 2 Chapter 2 entitled Warranty Return it to your nearest sales service office or directly to Biotecnica Instruments S p A After appropriate evaluation Biotecnica or its branch office will provide the best solution to the problem VERIFICATION OF THE CONTENTS OF THE WOODEN CRATES In the Basic Package the BT2000 PLUS analyzer system is provided with the following items Contents of Large Wooden Crate BT 1000 amp BT 2000 PLUS Qty Description OK ANALYZER USER S MANUAL INSTALLATION DISK WINDOWS SOFTWARE DISK AND LICENSE KEYBOARD DRIVER UPS DRIVER PRINTER DRIVER A A A A A A DISK OF PARAMETERS Contents of Small Wooden Crate BT 1000 amp BT 2000 PLUS Oty Description OK 1 UPS UNIT 1100VA P N 330 2132 PRINTER IN ORIGINAL PACKAGE P N 330 2172 a A a CORDLESS KEYBOARD amp MOUSE P N 662 2057 HBT 2000 PLUS ONLY 1 MOUSE WITH 3 BUTTONS P N 330 2040 BT 1000 ONLY 1 KEYBOARD P N 330 2054 BT 1000 ONLY 1 WASTE PROBE 1 TRANSPARENT TUBE WITH FILTER FOR DISTILLED WATER 1 TRANSPARENT OVERFLOW TUBE SECTION A Page 10 of 11 Contents of Small Wooden Crate BT 1000 amp BT 2000 PLUS Qty Description OK CUVETTE WASHING SOLUTION 6x90 m
133. ws 1 screw the front left 2 screws on the underside supporting bracket 5 6 Repair and Replacement of the Funnel Pump or Check Valve Once the sampling needle is cleaned the waste fluid in the needle Washing Funnel Figures 5 1 and 5 13 is automatically transferred by the Funnel Pump FUNNEL Figures 5 1 and 5 13 to the external waste FUNNEL CHECK container The check valve Figure 5 13 on VALVE Vi the funnel side prevents accidental fluid suckback FUNNEL To repair or replace the pump simply unscrew PUMP it from the supporting bracket The check valve can be easily replaced by simply BARCODE disconnecting it from the waste tube and replacing it with a new valve ensuring the B om SERUM DEVICES Figure 5 13 same flow direction SECTIONV Page 16 of 16 SECTION VI READING STATION RESERVOIR LIQUID LEVEL PERISTALTIC PUMP A DILUTER WASHER PINCH VALVE DILUTER PINCH VALVE WASHER CUVETTE PLATE LIGHT SOURCE LAMP E PHOTOMETER JAN Za J lt KI PELTIER MODULE 3 E 4 CUVETTE MOTOR gt READING STATION MODULE Figure 6 1 6 1 General The reading station module is made up of the reaction cuvette positioning mechanism cuvette plate assembly light source washer module photometer module Peltier thermoelectric module HzO reservoir liquid level sensor LM35 integrated circuit temperature sensor for temperature

Figure 7-1

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