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SERVICE MANUAL - Frank`s Hospital Workshop

1. SZ PG Fig 2 12 2 21 2 6 2 Analog to digital converter See schema E33001A sheet n 1 The logarithmic amplifiers output voltage 7 U16 is applied to the A D converter s input VIN 11 U19 through a RC net formed by R56 and C93 This converter takes as a reference the U15 2 5 V voltage and is supplied at 5 V The voltages are reached from 15 V by means of the D16 and D17 zeners and bypassed using the capacitors C80 C81 C83 and C84 The capacitors C99 C100 C101 and the resistor R60 belong to the converter circuit This capacitors must be of polypropylene type in order to have very low leakage currents As the voltage in 7 U16 can vary in the 15 V range while the input voltage of 11 U19 cannot exceed the U19 supply voltage 5 V the circuit formed by D8 D9 D10 D12 R43 and R44 protect it against overvoltages 2 6 3 Lamp control See schema E330014 sheet n 2 The lamp s power supply is done through the circuit formed by the RG1 regulator It receives the non regulated voltage from the 0 15 Vac transformer s coiling This voltage is
2. Fig 5 3 5 6 5 4 Printer replacement The printer is fixed to the case by means of a metallic support To change the printer proceed as follows see figure 5 3 a Disconnect the connectors J4 J6 and J7 from the display board b Remove the two screws 2 c Remove the four screws 3 To place the printer again proceed as follows a Fix the printer to the metallic support with the 4 screws 3 b Fix the support to the case with the 2 screws 2 c Connect the connectors J4 J6 and J7 of the display board 5 5 Keyboard replacement The keyboard is glued to the case and when removed it cannot be used again Because of this be sure that really it doesn s work properly before replacing it To change it proceed as follows see figure 5 4 a Disconnect the keyboard band from the display board J10 b Carefully unstick the keyboard from the case C Clean the remainded glue with alcohol d Fix the new keyboard e Connect the new keyboard to the display board J10 5 7 5 8 77 S 2 S 9 Fig 5 4
3. 5 6 Transformer replacement The transformer is fixed to the base To change it proceed as follows see figure 5 2 a Unsolder the supply wires b Disconnect the main board s connector J4 and the tab in terminal 13 c Remove the four screws 4 d Place the new transformer fixing it to the base with the four screws 4 e Solder the supply wires see schemes E33000A and E330014 f Connect J4 to the main board and the ground tab in terminal 13 Very important In order to meet the electrical safety standards CE mark requirements the solderings must be hook type made and the ground connections must be properly done The manufacturer declines all responsibility due improper handling made without following the above instructions 5 7 Cuvette holder tray removal To manipulate certain elements of the optical system it is necessary to remove the tray that supports the pump Proceed as follows see figure 5 2 a Remove the case see section 5 1 b Remove the waste tube from the outlet 8 in the lower part of the tray c Disconnect the peristaltic pump motor strip J3 from the main board d Remove the 2 screws 6 and the screw 7 that fixes it to the optical support e Remove the tray To place the tray again a Place the tray and fix it to the optical support with the 2 screws 6 and the screw 7 b Connect the peristaltic pump motor strip J3 to the main board C Connect the waste t
4. The instrument prints the following data Reading number Absorbance applying the calibration Absorbance without applying the calibration Reference value theoretical value of the sample Absolute and relative errors applying the calibration Absolute and relative errors without applying the calibration 4 11 Unlock Lock QC techniques When leaving the factory some memory locations contain the techniques used for the in house quality control They are de activated if they are activated again the names of this techniques will appear again in the list of programmed tests This option is for internal use and has no interest for the Technical Assistance Service 4 5 5 MAINTENANCE 5 1 Case replacement If a case s replacement is needed or it must be removed to operate the instrument s inside proceed as follows figure 5 1 a b O 2 With the instrument turned off remove the supply wire Remove the printer paper roll and take the aspiration tube out its guiding tube Remove the two screws 1 located in the lower part of the instrument Remove the two screws 2 located in the rear part of the instrument Carefully lift the case inclining it a bit to the right side and disconnect the supply strip J7 as soon as you have room enough to introduce your hand to the inside in PCB version 133002A080499 also disconnect the J11 flat band Then incline the case to the right side totally t
5. thermal bi directional Characters per line 40 Print speed 40 characters per second Paper thermo sensitive 80 mm wide 1 4 Display Type 320 x 240 dots graphic LCD with CFL backlight l 1 1 5 Electronics Microcontroller H8 3003 16 32 bits at 16 MHz FLASH memory up to 2 Mbytes RAM memory up to 512 Kbytes Amplifier Logarithmic amplifier hybrid circuit LOG 100 Scale factor K 1V Abs Without analog adjustments A D Converter Type dual slope Resolution 10 000 counts Abs Conversion time 150 ms at 2 Abs Adjustments by software 1 6 Communications Serial connection channel bi directional RS 232 Baud rate 110 150 300 600 1200 2400 4800 9600 19200 1 7 Installation Voltage 115 V tolerance from 99 to 137 V 230 V tolerance from 198 to 264 V Frequency 50 60 Hz Maximum apparent power 126 VA Installation category Overvoltage category Il Class A criterion C 1 8 Dimensions and weight Height 150 mm 5 9 in Width 387 mm 15 2 in Depth 337 mm 13 3 in Weight 9 5 Kg 21 Ib 1 9 Cuvette systems Flow cuvette 18 uL Common cuvettes macro semi micro and micro Round tubes 412 75 mm max height 1 2 1 10 Flow system Peristaltic pump System tube stretching Peristaltic tubing silicone density 60 shore Rotor 4 rollers Motor Stepping 100 steps turn Supply 1 A constant current Volume programmable f
6. Allows the accuracy s checking at a given temperature To select this option press the function key CHECK in the UTILITIES SERVICE Password ADJUSTMENTS HEATING menu The cursor appears in the line TEMPERATURE TO CHECK Insert the calibrated digital thermometer s temperature probe into the cuvette holder Input the temperature to be checked and press ENTER The message Heating is displayed with a time counter going down After the five minutes the screen s prompt asks for a new temperature Check the reached temperature in the external thermometer 3 2 3 Filters wheel adjustment This procedure describes the way to adjust the optimal position of the filters in the optical path of the reading group The theoretical position is established by the wheel reference photodetector but because of the mechanical tolerances a further correction is necessary to optimize centering 3 5 3 2 3 1 Materials needed Soft paper WYPALL LITE 56 g cm SCOTT Washing solution Code 20103 Alcohol Ether 50 50 solution Assay tubes Distilled water Closed circuit flow thru cuvette 3 2 3 2 General remarks Switch the instrument on at least 20 minutes before beginning the adjustment This procedure can be performer either automatic or manually The automatic procedure is recommended The adjustment is performed with the filter in the position n 1 340 nm Clean the outer faces of the
7. alcohol Resin remaining in this point may cause instability of the reading system Fix the photodiode wire to the board with a plastic clamp Connect all the strips again 5 3 Change of the display board See figure 5 3 a o a O 2 Disconnect in the board itself the keyboard band J10 and the printer connectors J4 J6 and J7 Disconnect the flat bands J1 and J5 supply connector J3 and the led connector J9 not available in PCB version I3300A080499 Remove the 4 screws 1 and the board To place the printed circuit board again proceed as below Place the board taking into account that the LED should be properly placed 5 3 f Fixthe board with the 4 screws 1 g Connect the keyboard strip J10 and the printer connectors J4 J6 and J7 h Connect the flat bands J1 and J5 the led connector J9 not available in PCB version 13300A080499 and the supply connector J3 5 4 n Cox en X I TT gt 1 LI SS b dd
8. and makes the voltage increase when it becomes hot In this way the higher is the load or the external temperature the faster is the fan s operation thus achieving a more silent function under normal conditions 2 6 14 Microcontroller See schema E330014 sheet n 1 The microcontroller circuit is formed by the H8 3003 Hitachi microcontroller U13 It is a last generation microcontroller incorporating peripherics such as I O lines counters ACIA s and a 10 bit A D converter with eight analog channels among others thus minimizing circuitry The microcontroller has internal memory and control signals to connect external memories Both code and data are stored in the 2 Mbytes FLASH memory U11 A 512 Kbytes RAM U12 is also used This RAM is provided with a battery powered supply voltage Vbat 2 26 The chip select signals ared decoded in the CPLD U9 U10 is intended to connect a EPROM 27C4001 If the jumper JP2 is closed the microcontroller seeks for the code in the EPROM U10 instead of in the FLASH U11 Usually U10 is used only by the manufacturer to load the monitor program in U11 the first time Afterwards it is no longer usefull at all 2 6 15 Power supply See schema E330014 sheet n 2 The power supply is in charge of supplying the voltages and currents needed in the different parts of the circuit The lamp supply has been described in section 2 6 3 and the fan supply in section 2 6 13 Besides th
9. channel it is necessary to fit the configuration parameters with the computer ones If the computer parameters are not known refer to its manual Usually they are likewise programmable in most cases from the application programs themselves The instrument can be programmed with the next parameters transmission speed bauds timeout and terminal number In the user s manual is explained how to program these parameters 2 16 PIN SIGNAL NC RXD TXD NC GND NC NC NC NC SERIAL PORT COM 1 o Omm GN x Quality control values Test s name Control 1 or Control 2 Control s name Control s lot Concentration Alarm Concentration 1 Alarm 1 Concentration 2 Alarm 2 Concentration n Alarm n Concentration values Tests Sample s Blank Factor Control 1 Concentration name number or Control 2 patient s or sample code id Fig 2 11 2 5 Block diagram The block diagram figure 2 12 is intended to give a general overview of the the electronic circuit s different parts 2 5 1 Logarithmic amplifier It converts the electric current coming from the photodiode in a voltage equal to its logarithm section 2 1 4 2 5 2 Analog to digital converter It digitalizes the voltage coming from the logarithmic amplifier for its further treatment by the microcontroller 2 5 3 Lamp control I
10. cuvette with the ethanol ether solution and dry with the WYPALL LITE paper Insert the cuvette into its holder Wash the inside of the cuvette by performing a washing with 1 mL washing solution and then abundant distilled water with the key WASH Check the absence of air bubbles in the inside of the cuvette by taking if out of its lodging and looking through 3 2 3 3 Manual mode When entering in this menu UTILITIES SERVICE Password ADJUSTMENTS FILTERS WHEEL the display shows the programmed values Their meaning is as follows SENSITIVITY indicates the current reading of the photometric sensitivity in the current filter position STEP indicates the number of steps that the wheel has done from the reference photodetector until the current position of the filter n 1 The increase or decrease regarding to the number without correction is indicated between brackets Press the function key MANUAL and using the up and down cursor keys increase or decrease the steps until the maximum sensitivity position If several consecutive steps give the same sensitivity leave it in the center of the range Validation criteria Check that the increase or decrease in steps in within the range indicated in Table 11 2 in Appendix ll Otherwise repeat the procedure 3 6 Once the adjustment completed press the function key EXIT and answer YES to the question Save values To exit the process without s
11. e Solve the problem when push the WASH key at the end of thermostating process the device reset Versi n 2 5 This version solves the next reported errors e Solve the problem with the WASH key and absorbance menu In this case the sip cycle cut up the sample Versi n 2 6 The next improvements have been added e Polish language is included This version solves the next reported errors VII 3 When quality control was made in the kinetic mode will take all the following samples as a quality control When the key WASH was pressed and thermostating process is working at the end of the cycle the device reset When at the same time work the printer the thermostating process and the key WASH was pressed the device will block Versi n 2 7 The next improvements have been added Save the memorised values of blank and factor in flash memory Save the result values concentration in the flash memory Added in the menu tools default values a soft key to delete in a manual maner all the concentration values Print the checksum values in the autotest This version solves the next reported errors When quality control was made in the kinetic mode the next sample is delayed while is saving the data When Chinese language is selected there was a communication error Versi n 2 8 This version solves the next reported errors From test number 10 there weren t the filters wheel initialisation Now this initialis
12. is equipped with watertight joint in order to prevent penetration of liquid into the inner part of the instrument If liquid is spilt into the tray clean it with damp paper or cloth A drainage hole connected to the outside by silicone tubing is in the front left part of the tray to facilitate drainage of poured liquid d If a cuvette breaks into the cuvette holder or for any reason liquid pours into it the holder has a drainage hole connected to the outside It will however be necessary to rinse and dry the inside of the cuvette holder e Cover the instrument with its dust cover when not in use 5 18 TRES SS E CN Fig 5 8 5 19 5 19 Cleaning of the optical components The following indications should be taken into account for the cleaning of the optical components a Optical components to be considered lamp lenses filters and photodiode b Recommended material Paper WYPALL LITE 56 gr m2 SCOTT Alcohol ether 50 50 solution Cotton ear picks Lens cleaner blower type C For a proper manipulation of the optical components the following general precautions should be taken into account The area for manipulating the instrument should be clean and in order As the components are fragile they should be treated carefully a fall could result in breakage Avoid touching the active areas with the f
13. rectified by the bridge D2 and filtered by C14 and C15 C17 is the bypass capacitor The 12V output voltage is given by the resistors R19 and R20 2 6 4 Filter wheel detector See schemes E33009A and E330014 sheet n 1 The wheel detector consists in the photointerrupter MCT 81 according to the schema E33009A and it is mounted on the printed circuit reference 1363 The schema E33001A shows the resistor R46 connected to the emitter photodiode s anode as a power supply and the resistor R41 connected to the collector as a load From this point the logic signal is taken and read by the microcontroller pin PC4 15 U13 Under normal conditions the photodiode s infra red light reaches the phototransistor which starts to work in saturation mode and the signal in its collector is a logic 0 When the filters wheel turns and the detection stem interrupts the light from the photodiode the transistor stops conducing and the signal in its collector is a logic 1 2 22 2 6 5 Filters wheel motor control See schema E33001A sheet n 2 The filters wheel stepper motor control is achieved by two integrated circuits PBL3717A U5 and U6 These circuits are constant current stepper motor drivers Each one supplies the current to one of the motor coils This current depends of the reference voltage applied in the REF pins 11 U5 and 11 U6 This voltage is generated by the 12 V zener D3 the resistors R23 in U5 and R24 in U6 and the internal
14. thermal contact between the temperature sensor and the cuvette holder To ensure proper conditions in both points the following cautions should be taken into account a A thin uniform layer of silicone must be placed in each face of the Peltier Cell covering the whole surface b The silicone layer should neither go beyond the surface of the Cell nor go inside between its two faces it would result in a thermal short crossing that would reduce the system s performance C The temperature sensor should have a silicone layer in order to make a good thermal contact with the bottom of its lodging For assembling these components follow the procedures described in sections 5 11 changing the Peltier Cell and 5 14 changing the temperature sensor 2 14 2 4 Communications system This instrument is equipped with a communication channel allowing connection with computers The operation of this communication channel depends on the program release and the computer s application software 2 4 1 Channel type The communication channel is a RS 232 and uses the following connection lines RxD Receiver Data TxD Transmitter Data GND Ground 0 volts The connector is located in the instrument s back side rounded by a box labeled COM 1 The signals electric level meets the E l A RS 232 standard Figure 2 10 describes the connector s signals 2 4 2 Channel characteristics The serial channel is pre programmed by the man
15. to adjust with different filters Adjustment process is described in section 3 2 2 1 6 Precautions and maintenance Maintenance of the optical system should be carried out according to the instructions given in section 5 20 2 2 Aspiration system 2 2 1 Components The aspiration system is composed of the following parts figure 2 5 a Sipping tubing 1 b Flow cuvette 2 C Peristaltic pump 3 d Waste bottle 4 2 2 2 System description The sample is sipped by the sipping tubing 1 This tubing Teflon made has a standard length and the system is adjusted in accordance with it The sipped sample enters into the flow cuvette 2 where readings take place Sipping is performed through a silicone tube by means of a peristaltic pump 3 made up of a four rollered rotor that is contolled by a stepper motor Finally the sample ends into the waste bottle 4 2 7 2 2 3 Physical description The sipping tubing figures 2 6 and 2 7 is placed on the cuvette holder tray The silicone tube 1 that crosses the case through a steel guiding tube 2 is fixed to this guiding tube by means of a silicone connector 3 and to the cuvette 4 by means of an inlet adapter 5 The teflon tube 6 is connected to the cuvette by an outlet adapter 7 placed in the peristaltic pump 8 and finally connected to the waste outlet 9 The waste bottle 1 figure 2 8 is connected to the outlet 2 in the back of the instrument by me
16. volume in L to be checked and press ENTER The message Insert tube with water and press PUMP is displayed Insert a tube with water in position of aspiration and press PUMP 3 2 4 3 Check method SAMPLE VOLUME Fill an assay tube with distilled water and weigh it in an analytical balance Weigh it again after an aspiration cycle The difference in grams is equal to the volumen in mL POSITIONING When the aspiration cycle is completed check that there is a 5 mm tail 0 10 mm of the sample that has not yet entered the cuvette Use the caliper square if necessary 3 2 4 4 Manual mode Insert the flow thru cuvette in its holder making sure that its position is correct SAMPLE VOLUME It corresponds to the adjustment of the sipped volume Insert the number of quarters of step that the pump must perform to sip 5 mL and press ENTER The theoretical value is 18340 equivalent to 1 09 L per step the motor works with quarters of step 0 2725 per each quarter of step The number to input is an estimate and should be determined by error and trial using the function CHECK of the menu ADJUST PUMP 3 9 POSITIONING It corresponds to the adjustment of the positioning of the sample Input the number of quarters of step needed for the sample to be positioned into the cuvette with a tail of only 5 mm 0 10 mm without entering into the cuvette and press ENTER The theoretical value is 600 quarters of step The number is an es
17. 20 125 405 60 800 420 100 1000 450 200 2000 492 300 8000 505 500 4000 530 750 5000 546 800 5500 578 900 9000 600 1000 10000 630 1750 13000 670 2000 14000 11 2 11 7 Fan voltage control Maximum 24 v Minimum 15v 11 8 Zero currents for the sensitivity test Maximum 30000 nA Minimum 10nA 11 9 Heating adjustment Once thermostatization is adjusted the temperature should have a stability of 0 2 C 11 10 Peristaltic pump adjustment The trail of the sample that remains in the aspiration tubing before entering into the cuvette should be between 0 and 10 mm If those values are not reached follow the MANUAL procedure NOTE All the values are statistical and can suffer variations when new production series will be released Thus the case this appendix will be updated Il 3 APPENDIX Ill DEFAULT PARAMETERS PERSONALIZATION LABORATORY NAME VOID LANGUAGE ENGLISH PRINTER THERMAL PRINTER PRINTER CONTRAST 7 WASH MODE CONTINUOUS LIST HEADING COMPLETE FILTERS TABLE A RECON RN 340 IE 405 c 420 Audit 505 ONE ERAS 546 Docs 578 A O 670 EEE RIO RAEE N UNITS TABLE 0 mg dL 1 U L 2 g L 3 kat L 4 e mol L 5 mmol L 6 e g L Ta nkat L 8 g dL 9 e g dL 10 Ul mL 11 COMMUNICATIONS CONFIG RS 232 1 4 APPENDIX IV MAINTENANCE PLAN In order to maintain the proper operation conditions it is reco
18. 3 3 3 3 3 3 4 3 4 3 4 3 5 3 5 3 5 3 6 3 6 3 6 3 7 3 8 3 9 3 9 3 9 3 9 3 2 4 5 Automatic mode 3 3 Transformer and power supply checking 3 4 Fan checking 3 5 Optical system checking 3 6 Check of the sensitivity with flow cuvette 4 CHECK TESTS 4 1 Activation of a test 4 2 Screen 4 3 Buzzer 4 4 Keyboard 4 5 Printer 4 6 Serial Port RS 232 4 7 Motors 4 7 1 Loss of steps of the peristaltic pump 4 7 2 Loss of steps of the filters wheel 4 8 Cuvette temperature 4 9 Carry Over 4 10 Photometry 4 10 1 Filter sensitivity 4 10 2 Electric noise 4 10 3 Stability of the readings 4 10 4 Precision 4 10 5 Accuracy 4 11 Unlock Lock QC techniques 5 MAINTENANCE 5 1 Case replacement 5 2 Main board replacement 5 3 Change of the display board 5 4 Printer replacement 5 5 Keyboard replacement 5 6 Transformer replacement 5 7 Cuvette holder tray removal 5 8 Filters wheel replacement 5 9 Filters wheel motor replacement 5 10 Peristaltic pump replacement 5 11 Peltier cell replacement 5 12 Change of the photodiode 5 13 Fan replacement 5 14 Temperature sensor replacement 5 15 Lamp replacement 5 16 Filters replacement 5 17 Lenses replacement 5 18 General care 4 5 5 19 Cleaning of the optical components 5 20 Cleaning the filters 5 21 Cleaning the lenses 5 22 Cleaning the photodiode 5 23 Cleaning the aspiration system 5 24 Cleaning the flow cuvette
19. 5 25 General cleaning of the instrument APPENDIX I TECHNICAL SPECIFICATIONS 1 1 Optical system 1 2 Thermostatic control 1 3 Printer 1 4 Display 1 5 Electronics 1 6 Communications 1 7 Installation 1 8 Dimensions and weight 1 9 Cuvette systems 1 10 Flow system 1 11 Environmental conditions APPENDIX Il CALIBRATION TOLERANCES TABLES 11 1 Photometric adjustment Tolerances 11 2 Filters Wheel Adjustment 11 3 AC Voltage of the Transformer Secondaries 11 4 Filters sensitivity without flow cuvette 11 5 Electric Noise 11 6 Filters sensitivity with flow cuvette 11 7 Fan Voltage Control 11 8 Zero Currents for the Sensitivity Test 11 9 Heating Adjustment 11 10 Peristaltic Pump Adjustment APPENDIX III DEFAULT PARAMETERS APPENDIX IV MAINTENANCE PLAN IV 1 Cleaning IV 1 IV 2 Change IV 3 Review IV 4 Check 5 20 5 23 5 23 5 23 5 23 5 24 5 24 11 1 1 1 1 1 1 1 Il 2 Il 2 Il 2 Il 3 Il 3 Il 3 Il 3 111 4 IV 1 IV 1 IV 1 IV 1 APPENDIX V SPARE PARTS AND ACCESSORIES V 1 Accessories V 2 Authorized Spare Parts APPENDIX VI PASSWORD APENDIX VII SOFTWARE VERSIONS APENDIX VIII COMPATIBILITY TABLE TO UPDATE SOFTWARE VERSIONS ESQUEMAS Shematic distribution E33000A Monocard 1 4 E33001A Monocard 2 4 E33001A Monocard 3 4 E33001A Monocard 4 4 E33001A Display Printer 1 2 E33003A Display Printer 2 2 E33003A Keyboard 330 E33005A Photodetector E33009
20. 6 Peristaltic Tubing AC3114 Silicone Tubing 3x6 200 cm AC3495 Adapter for test tubes AC3703 Waste Bottle d Bottle 100 mL washing solution FU656 Fuse 1A AC3097 Fuse 2 A dei Supply Cable AC4461 Dust Proof Cover 810 LA2151 Halogen Lamp 12 v 20 w Fl4649 Filter Set 340 nm Fl4650 Filter Set 405 nm FI4651 Filter Set 420 nm Fl4658 Filter Set 492 nm Fl4652 Filter Set 505 nm Fl4653 Filter Set 546 nm Fl4654 Filter Set 600 nm Fl4659 Filter Set 620 nm Fl4655 Filter Set 670 nm FI5557 Filter Set 578 nm V 2 Authorized Spare Parts ii Cover St P C Board Micro 133002 ME7762 Optical Set Complet F BTS 330 DI2964 Photodetector S 1336 8BQ IN4342 Switch EATON E30M11J01 ZO648 Fuse Holder D 06 26 VA4343 Supply Filter FD 1Z DI844 Diode LM 335 MO3560 Approved fan ME3215 Fan Grid V 1 TR6813 IN4345 VA1467 Z0476 20474 Z04648 D14438 PC6825 IM3559 VA4413 AC4290 Transformer E33007A Tension Selector 18 000 0016 Terminal MOLEX 4809 Connector 2 54 H 4 Connector 2 54 H 3 Mechanized Lamp Holder Wheel Photodetector mounted P C Board Display I33004A Printer SEIKO STP312 F256 B Display Protector Keyboard Calibration Box Consult the Technical Assistance Service V 2 APPENDIX VI PASSWORD The PASSWORD to access the SERVICE menu is 251297 VI 1 APENDIX VII SOFTWARE VERSIONS Version 1 0 In this version the next errors have been reported e The Portuguese language does n
21. A MODIFICATIONS 1 Modification This is to make compatible in U7 the integrated circuits MAX691 Maxim or ADM691 Analog Devices Schemes 2 Modification This is to all devices to be only one monocard Schemes 3 Modification The problem is the transform of inverter circuit fails Photos Schemes V 1 V 2 VI 1 VII 1 VIII 1 4 Modification Modification for recode the components to improve their description 5 Modification Modification for change the display circuit board version Schemes VI 4 1 5 2 1 INTRODUCTION This instrument instrument due to its compact design and the reduced number of components is a simple easy to maintain one The computer design has allowed to study the tolerance of all the optical block s components making unnecessary any mechanical adjustment Most of its parts are aluminum or plastic injected and so a maximum simplicity with reduced maintenance requirements has been achieved Electronic adjustments are also avoided because of the modern high integrated circuits used A powerful software allows the adjustment of most of the parameters used Corrections are made via software using reference tools or calibrators All the electronic parts are located in two printed circuit boards PCB s and the software has a lot of check programs to make easy the search and diagnosis of failures This manual is not only a guide for maintenance but a do
22. AND ADJUSTMENTS In order to simplify the failure s diagnosis and adjustments there is included a set of test programs that allow the checking and adjustment of most of the functional parts With the only exception of the peristaltic pump adjustment accessible to the user the access to these programs is done through the menu UTILITIES SERVICE Then a password is required PASSWORD Input the password Appendix VII The display shows asterisks while typping In case of mistake press C to repeat 3 1 Service menu When selecting this option the following is displayed SERVICE 03 03 99 17 07 00 0 TEST 1 ADJUSTMENTS 2 UNLOCK LOCK TEST NLOCK OCK IN m 3 1 1 Option TEST By selecting this option one can access to the diverse tests available for failure diagnosis that appear in the following list Its function is described in Section 4 SCREEN BUZZER KEYBOARD 3 1 PRINTER SERIAL PORT MOTORS PUMP STEPS LOSS FILTER STEPS LOSS CUVETTE TEMPERATURE CARRY OVER PHOTOMETRIC FILTERS SENSITIVITY ELECTRONIC NOISE READINGS STABILITY PRECISION ACCURACY UNLOCK LOCK QC TESTS 3 1 2 Option ADJUSTMENTS By selecting this option one can access to the utility programs to perform the adjustments of the photometry temperature control filter wheel and peristaltic pump The diverse adjustment programs are described in Section 3 2 3 1 3 Option UNLOC
23. K LOCK TESTS This option allows opening and closing the diverse tests by selecting them either individually or all together The closed tests are not available to the user 3 1 4 Option UNLOCK LOCK instrument This option allows opening or closing the instrument s programming When activated the option PROGRAMMING disappears from the main menu thus making impossible for the user to modify the test and the units When the instrument is re opened the options allowing modification of tests and units are available again 3 2 ADJUSTMENTS 3 2 1 Photometric adjustment The aim of this procedure is to correct the absorbance values in the range 0 3 A with neutral filters previously calibrated in order to obtain results the same as with a reference spectrophotometer The final target is to obtain results within the tolerance range between 340 and 900 nm in the previously mentioned range of absorbances 3 2 3 2 1 1 Materials needed Verified calibration neutral filters Rubber bulb Cotton ear picks Alcohol ether 50 50 solution 3 2 1 2 General remarks Switch the instrument on 20 minutes before beginning the adjustment Before beginning take dust away from the neutral filters using the rubber bulb 3 2 1 3 Procedure Enter in the UTILITIES SERVICE Password ADJUSTMENTS PHOTOMETRIC menu Program the values requested in the first display Press the function key CHECK VALUES Pres
24. SERVICE MANUAL MS3301A031203 INDEX 1 INTRODUCTION 2 FUNCTIONAL PARTS DESCRIPTION 2 1 Optical system 2 1 1 Constitutive parts 2 1 2 System description 2 1 3 Physical description 2 1 4 Signal conditioning 2 1 5 Adjustment 2 1 6 Precautions and maintenance 2 2 Aspiration system 2 2 1 Components 2 2 2 System description 2 2 3 Physical description 2 2 4 Programmable parameters 2 2 5 Programming 2 2 6 Adjustment 2 2 7 Precautions and maintenance 2 3 Thermostatization system 2 3 1 Components 2 3 2 System description 2 3 3 Physical description 2 3 4 Programming 2 3 5 Adjustment 2 3 6 Precautions and maintenance 2 4 Communications system 2 4 1 Channel type 2 4 2 Channel characteristics 2 4 3 Information transmitted 2 4 4 Programming 2 5 Block diagram 2 5 1 Logarithmic amplifier 2 5 2 Analogic Digital converter 2 5 3 Lamp control 2 5 4 Filters wheel detector 2 5 5 Filters wheel motor control 2 5 6 Temperature sensor amplifier 2 5 7 Peltier cell control 2 5 8 Pump motor control 2 5 9 Keyboard circuit 2 5 10 Printer control 2 5 11 Display circuit 2 5 12 RS 232 channel circuit 2 5 13 Fan control 2 5 14 Microcontroller 1 1 2 1 2 1 2 1 2 2 2 7 2 7 2 7 2 7 2 8 2 8 2 8 2 9 2 9 2 9 2 14 2 14 2 14 2 14 2 15 2 15 2 15 2 15 2 16 2 18 2 18 2 18 2 18 2 18 2 18 2 18 2 18 2 19 2 19 2 19 2 19 2 19 2 19 2 19 2 5 15 Powe
25. aking care that the connecting strip that joins it to the main board is not stretched In case it to be fully removed disconnect the flat bands J19 and J10 from the main board and the printer and display ground tab in terminals 12 from fig 5 2 It is recommended to remove the cuvette holder cover to avoid it falling down To place the case again or to put a new one a b Connect the flat bands J9 and J10 again and the tab in terminals Place the case again carefully connecting the supply strip J7 before closing the case in PCB version I33002A080499 also connect the J11 flat band Screw the fixing screws Place the printer paper again and the aspiration tube figures 2 6 and 2 7 Place the cuvette holder cover if it was removed 5 1 fa nn Fig 5 1 5 2 5 2 Main board replacement See figure 5 2 Disconnect all the strips reaching the board Cut the clamp that fixes the photodiode cable Unsolder the photodiode cable Remove the 5 Allen screws 1 fixing the radiator to the base Remove the three screws 2 fixing the main board to the respective separators To mount the board again proceed as follows e Put a thin and uniform silicone layer in the lower part of the heat sink 3 Place the board again and fix it with the five Allen screws 1 and the three screws 2 Solder the photodiode wire thoroughly cleaning the solder resin with
26. ans of a silicone tube 3 2 2 4 Programmable parameters Three parameters control the sipper functioning and they must be programmed to obtain the expected performance characteristics Their programming is explained in the user s manual a SAMPLE VOLUME It is a number that indicates the volume of sample to be sipped in ul b PUMP DELAY It is the number of seconds the pump will wait since the sipping finishes till the pump is activated again to position the sample into the flow cuvette c POSITIONING It is the number of steps that the peristaltic pump s motor gives to set the sample into the flow cuvette ensuring that it is suitably positioned in order to be read 2 2 5 Programming The aspiration system s programming must be performed in order to indicate the instrument the volume in microlitres to be sipped 2 2 6 Adjustment The pump s nominal flow is 110 uL revolution nevertheless the accuracy of this figure depends on the tolerances in length and diameter of the sipping tube that may be affected by the aging of the tube itself this is why this value should be adjusted from time to time The adjustment process is explained in section 3 5 As it can be also done by the user it is also explained in the user s manual 2 8 2 2 7 Precautions and maintenance The general rules for the aspiration system s maintenance are the same as the ones given in section 5 24 Weekly adjustment of the aspiration system
27. ans of the screwed ring using the tool indicated in point e 5 17 Lenses replacement Lenses are mounted in holders to make it easier to handling them To change a lens holder set proceed as follows see figure 5 2 a Remove the case as indicated in section 5 1 b Remove the cuvette holder tray as indicated in section 5 7 c Remove the filters wheel as indicated in section 5 8 d Remove the light guide 12 e Using the corresponding tool from the SERVICE TOOLS KIT code 005 unscrew the lens holder set to be removed 4 or 11 5 17 To place it again proceed in the opposite order If it is necessary to take the lens away from the inside of the filter holder set proceed as follows see figure 5 10 f Using the corresponding tool 4 from the SERVICE TOOLS KIT code 005 unscrew its fixation ring 3 g Remove the lens 2 from its holder 1 taking care not to touch its faces with the fingers h Insert the new lens and fix it in the holder with the screwed ring using the tool indicated in point f 5 18 General care In order to get an optimal operation of this instrument it is necessary to follow some minimal maintenance rules a Never use detergents or abrasive products for cleaning the outside of the instrument Use only a cloth with water and neutral soap b If a reagent or any corrosive product is spilt on the apparatus clean it immediately with a damp cloth and water C The cuvette holder tray
28. ation is done when the equipment is switched on Only in the Chinese language when was edited a name with a character code 181 then this character will change to a letter mu u Now the error is corrected When you switch on the equipment and enters immediately in the menu absorbance the read filter had a zero value now is initialised at 340 VII 4 APPENDIX VIII COMPATIBILITY TABLE TO UPDATE SOFTWARE VERSIONS This table show the different way to update the software versions There are two ways to update the version One is save the program from the EPROMS memories The second one is from the PC through a serial channel The first column shows the actual version of the program The first row shows the version you want to update Each cells shows how you can update the version 2 1 2 2 2 3 2 4 2 5 2 6 2 7 2 8 2 0 Eprom Eprom Eprom Eprom Eprom Eprom Eprom Eprom 2 1 Eprom Eprom Eprom Eprom Eprom Eprom Eprom 2 2 Eprom Eprom Eprom Eprom Eprom Eprom 2 3 Eprom Eprom Eprom Eprom Eprom 2 4 Eprom PC Eprom PC Eprom PC Eprom PC 2 5 Eprom PC Eprom PC Eprom PC 2 6 Eprom PC Eprom PC 2 7 Eprom PC VIII 1
29. aving thus keeping the former values answer NO or press ESC instead of EXIT Press the function key EXIT again Select the sensitivity test by the following path UTILITIES SERVICE Password TESTS PHOTOMETRIC FILTERS SENSITIVITY WARNING Check that the test is performed with the cover closed The reading process for each filter begins A list of the sensitivity nA obtained for each filter is printed The orientative ranges appear in table 11 6 of Appendix Il In the case the results are considered not valid verify the following points The cuvette model is adequate and the distance between the light path hole and the base of the cuvette is Z 8 5 mm The inside and outside of the cuvette is clean There are no bubbles inside the cuvette The connections of tubing and adaptors are tight The teflon tubing is not strangled The filters wheel is properly positioned Press the function key EXIT wash with distilled water and air the flow thru cuvette by pressing the key WASH put it in the stand by position and loosen the peristaltic pump silicone tubing 3 2 3 4 Automatic mode When entering in this menu UTILITIES SERVICE Password ADJUSTMENTS FILTERS WHEEL the display shows the programmed values Their meaning is as follows SENSITIVITY indicates the current reading of the photometric sensitivity in the current filter position STEP indicates the number of steps that the wheel has
30. c Remove the pump and substitute it fixing the new one with the 4 screws 5 d Connect the peristaltic pump motor strip J3 to the main board again 5 11 Peltier Cell replacement See figure 5 2 a Remove the cuvette holder tray as described in section 5 7 b Remove the temperature sensor 16 c Remove the photodiode 20 d Disconnect J2 from the main board e Unsolder the Peltier Cell wires from those going to the connector J2 f Remove the 4 screws 4 9 Carefully remove the cuvette holder 13 h Remove the Peltier Cell 15 i Take a new cell and place a thin and uniform silicone layer on both faces j Place the cell in its position taking into account that its black cable should be in the front part of the optical system k Place the cuvette holder and fix it with the 4 screws 14 During this operation take special care to maintain the cuvette holder parallel to the cell and the cell parallel to the optical support and progressively screw the four Screws in order to avoid breakage of the ceramic faces of the cell I Once the cell is positioned check that no silicone overflows that could thermally short circuit the two faces of the cell Thus the case clean it carefully m Solder the cell wires again to those of the connector J2 according to the colour codes 5 11 n Place the temperature sensor 16 o Place the photodiode 20 p Place the cuvette holder tray again as described in se
31. center tapped coiling Each triac can let a positive or negative half wave pass when convenient The circuit formed by the amplifier TLO72CD U3A that works as a comparator and the transistor BC548 T5 that makes the output 2 23 1 U3A 15 V adequate to logic level 0 at 5 V form a polarity detector for the alternating voltage that supplies the power control circuit This signal is applied to the microcontroller by the pin P71 87 U13 in order to know which triac and when should be activated The microcontroller activates the triacs by means of two signals leaving by pins PA6 111 U13 and PA7 112 U13 and that together with the polarity detection signal collector T5 are applied to the programmable logic circuit XC9572 PC84 U9 to generate the two triacs control signals 77 U9 and 6 U9 and avoid a simultaneous activation caused by a program error Circuits 2 U1A 4 U1B and the transistors T1 and T3 form the triacs activation circuit Should it occur the fuses F3 and F4 in the common coil protect the transformer The result of this procedure is that each triac supplies the Peltier Cell a half wave in the same sense depending on the heating or cooling requirements 2 6 8 Pump motor control See schema E330014 sheet n 2 This circuit formed by the integrated circuits U2 and U4 is similar to the one used for the filters wheel control the only difference between them is the R8 and R10 resistor values because the curr
32. control and supply lines one inverter circuit for the CFL backlight and one circuit to control the LCD contrast adjustment 2 5 12 RS 232 channel circuit It is formed by the circuits needed to make the signal conditioning from TTL voltage level to the RS 232 standard The ACIA is integrated in the microcontroller itself 2 5 13 Fan control This is a circuit that measures the temperature in the power supply s heat sink and changes the fan s speed according to the cooling necessity A more silent working conditions are thus achieved 2 5 14 Microcontroller By means of its program it is in charge of linking and controlling almost all the instrument systems Only the fan s circuit and the lamp s one are not controlled by the microcontroller 2 19 2 5 15 Power supply It is in charge of supplying the needed voltages to the different instrument parts 2 5 16 Reset and battery backup supervisory circuit When the instrument is turned on this circuit is in charge of keeping the microcontroller and other chips like memories in a reset state until the supply voltage reaches the proper working level avoiding undesired effects when the instrument is powered on as well as protecting the RAM against erroneous writings during the on off voltage transitions Also this circuit provides a batery backup switchover every time the instrument is switched ON OFF 2 6 Electronic circuit description This section describes the diff
33. ction 10 7 5 12 Change of the photodiode See figure 5 2 a Remove the case as indicated in section 5 1 b Remove the screw fixing the ground terminal 21 to the cuvette holder c Remove the photodiode set unscrewing the support 20 If a change of the photodiode is necessary proceed as follows see figure 5 6 d Take the support 3 and the separator 2 away backwards e Remove the insulating ring 5 f Unsolder the wire from the photodiode pins 7 g Solder the new photodiode 1 h Place the insulating ring again 5 i Move the support 3 forward until the separator 2 contacts the photodiode j Screw the set again to the cuvette holder k Fix the ground wire 6 to the cuvette holder again I Place the case again as explained in section 5 1 5 12 Y A 5 13 Q Y Fig 5 5 5 13 Fan replacement See figure 5 2 a Remove the case as indicated in section 5 1 b Disconnect J8 from the main board c Remove the 4 nuts 9 d Remove the grid 10 e Remove the fan f Place a new fan taking into account that the air flux is outwards 9 Place the grid again 10 and fix the set with the 4 screws and nuts 9 h Connect J8 again to the main board i Place the case again as explained in section 5 1 Very important In order to meet the electrical safety standards CE mark requirements the fan grid resistance to ground must be equal or less than 0 10 T
34. cument for Technical Assistance Service staffs training The running principles as well as the electronic circuits are explained in order to get a global view of the instrument Note Throughout this manual the Absorbance Unit is abbreviated as Abs instead of A in order to avoid confusion with the electric current unit the Ampere represented as A 1 1 2 FUNCTIONAL PARTS DESCRIPTION This instrument is composed of the following functional parts a An optical system to carry out the readings b An aspiration system based on a peristaltic pump to introduce the sample in the flow cuvette c A thermostatic system to carry out reactions into the cuvettes at a constant temperature when needed d A communications interface allowing the connection to a host computer by means of a proper software e A microcontroller based system wich controls most of funtional parts The next sections describe each one of the functional parts 2 1 Optical system 2 1 1 Constitutive parts Figure 2 1 shows the optical system lt consists in a An halogen lamp 1 b A diaphragm 2 C A first plano convex lens 3 d An interference filter 4 e A second plano convex lens 5 f A cuvette 6 9 A silicon photodiode 7 2 1 2 System description The first component is the light source one halogen lamp 1 The diaphragm 2 delimits a light solid angle that is collected by the first lens 3 lt sends a parallel beam t
35. d The filters wheel is properly positioned Press the function key EXIT wash with distilled water and air the flow thru cuvette by pressing the key WASH put it in the stand by position and loosen the peristaltic pump silicone tubing 3 2 4 Peristaltic pump adjustment This procedure describes the way to adjust the flow of the peristaltic pump and the positioning of the sample in the cuvette The pump flow depends on the number of steps done by the motor and on the internal diameter of the peristaltic tubing Slight differences in the internal diameter in the different tubes may result in variations in the flow that can also be affected by slight deformations caused by repeated use Also slight differences in length or diameter can affect the positioning of the sample This adjustment is intended to compensate the variations due to these tolerances and the aging of the tubing 3 8 3 2 4 1 Materials needed Volume adjustment tool 5 mL pipette 0 05 mL mark Analytical balance optional Caliper square optional 3 2 4 2 General remarks Switch the instrument on at least 20 minutes before beginning the adjustment This procedure can be performer either automatic or manually The automatic procedure is recommended The function CHECK allows input of a volume between 100 and 5000 that are checked by aspiration cycles When selecting this option the cursor appears in the line CHECK VOLUME L Input in the
36. done from the reference photodetector until the current position of the filter n 1 The increase or decrease regarding to the number without correction is indicated between brackets Press the function key AUTOMATIC The instrument with look for the position with maximum sensitivity 3 7 Validation criteria Check that the increase or decrease in steps in within the range indicated in Table 11 2 in Appendix ll Otherwise repeat the procedure Once the adjustment completed press the function key EXIT and answer YES to the question Save values To exit the process without saving thus keeping the former values answer NO or press ESC instead of EXIT Press the function key EXIT again Select the sensitivity test by the following path UTILITIES SERVICE Password TESTS PHOTOMETRIC FILTERS SENSITIVITY WARNING Check that the test is performed with the cover closed The reading process for each filter begins A list of the sensitivity nA obtained for each filter is printed The orientative ranges appear in table 11 6 of Appendix Il In the case the results are considered not valid verify the following points The cuvette model is adequate and the distance between the light path hole and the base of the cuvette is Z 8 5 mm The inside and outside of the cuvette is clean There are no bubbles inside the cuvette The connections of tubing and adaptors are tight The teflon tubing is not strangle
37. e insert the lamp holder in its place Position the flange 2 and tighten the Allen Screw 3 h Place the case again as explained in section 5 1 5 15 LLLLLA 5 16 Fig 5 6 5 16 Filters replacement If a new filter must be installed it should be inserted in one of the two free positions of the filters wheel The filter will come fitted in a filter holder marked with the corresponding wavelength and thus it is only necessary to manipulate the filter holder Proceed as follows see figure 5 8 a Remove the case as indicated in section 5 1 b Insert the filter holder 1 in one of the free positions by simply pressing it Warning do not touch the filter faces with the fingers c Place the case again as explained in section 5 1 d Now it is necessary to add the new filter into the FILTERS TABLE To do it switch the instrument on and select the path UTILITIES SET UP FILTERS and add the wavelength value in the position where it has been inserted The filter is thus ready to be used If by any reason it is necessary to take the filter away from its holder proceed as follows see figure 5 9 e Using the corresponding tool 4 from the SERVICE TOOLS KIT code 005 unscrew the filter subjection ring 3 f Take the filter 2 away from its holder taking care not to touch the faces with the fingers 9 Insert the new filter taking into account the direction of the arrow 5 and fix it to the holder by me
38. ent in this motor is different Thus refer to section 2 6 5 for its description 2 6 9 Keyboard circuit See schemes E33005A E33003A and E330014 sheet n 1 The membrane type keyboard forms a contact matrix and detects the pressed key by scanning schema E33005A The keyboard is connected to the printed circuit 133004 by J10 The lines of this connector pass directly to the connector J5 schema E33003A that carries them to the printed circuit 133002 The scanning is generated in the lines referenced KBD 0 9 pins 65 63 62 61 58 57 56 55 54 and 53 of U9 XC9572 PC84 by the microcontroller that sends control commands to U9 using the data bus DB 0 7 and the address bus ADD 0 23 The line quiescent mode is the logic 1 Only one of them is activated by the logic O at one time The keyboard status is read by means of the lines KBDO a KBD4 65 63 62 and 61 of U9 If there is no key pressed the status is the logic high level because of the pull up resistor array AR2 When a key is pressed it is produced a contact with one of the scanning lines and logic low level is leaded to the associated read line The microcontroller detects the pressed keys by means of U9 using the data bus 2 24 Diodes D4 to D8 prevent from undesired short circuits between one scan line at 0 and other at 1 when pressing two keys at the same reading line simultaneously 2 6 10 Printer control See schemes E33003A and E33001A sheet n 1 T
39. erent electronic parts following the functional structure given in section 2 5 2 6 1 Logarithmic amplifier See schema E330014 sheet 1 The logarithmic amplifier is formed by the hybrid circuit LOG 100 U16 Its supply voltage is 15 V C90 C91 C96 and C97 are bypass capacitors that store electrical charge that is released to the power line whenever a transient voltage spike occurs The photodiode is connected between ground and the input I1 1 U16 A reference current section 2 1 4 is generated in the 2 5 V voltage regulator TL431CD U15 and the T circuit formed by R45 R51 and R52 Its nominal value is 100 nA The LOG 100 has the output OUT 7 U16 connected to the pin K1 3 U16 thus being the logarithmic conversion constant K equal to 1 The capacitor C92 is for circuit stability 2 20 3S LINDHIO AHOSIAHIANS AYHILLVEA ONVLISIH Alddns TOHLNOI NYA D ZEZ SH AVTdSIG M3INI amp d G3VOSA3M HIMOd JANNVHO YOLOW LINDHIO YOLOW dWNd 7149 4311134 HOSNIS JOSINUS IFUNLVATANIL 133HM H3LNH 40193130 JHL JO TOYINOD FHL JO TOMLNOD WES AHL HO amp SIHETHWV AHL AO TOULNOI 19IHMHILUH YILHIANOI a v HINATANY OIWHLIHVOOT
40. erference A cuvette holder 13 holds both the cuvette and the photodiode detector The thermostatic system is fitted together see section 2 3 2 1 4 Signal conditioning See figure 2 4 The photodiode 1 gives an electric current lp directly proportional to the received light This current goes to the input I of the LOG 100 logarithmic amplifier 2 A reference current 3 nominal 100 nA is introduced by input lo The output voltage of LOG 100 is In this case k 1 and Vo 1 volt Abs 2 2 2 4 Fig 2 2 2n 00 8H F odv VOOS DSL oA oA Y e idWv IINHLIYV9201 001 907 7 Fig 2 4 2 6 Vo is digitalized by the double ramp converter TSC500A 4 with a resolution of 10 000 counts per volt The conversion time depends on the absorbance value and increases together with it for instance 2 Abs time is 0 15 sec This converter is controlled by the microcontroller 5 2 1 5 Adjustment The global system optical system amplifier and converter is not strictly linear due to the components tolerance so that it is necessary to do some adjustments to compensate the inherent deviations of the system The photometric adjustment is performed in ranges between 0 and 2 Abs Several value ranges are determined and different correction factors are applied depending on the range As deviation also depends on the wavelength itis necessary
41. ese power supplies there is the 15 V one formed by the regulators RG2 and RG3 with their associated circuits and the 5 V one This power supply departs from a 0 to 9 V transformer coiling The alternate voltage is rectified by the bridge D4 filtered by C39 and C40 and the high frequency is bypassed by C50 The regulated voltage obtained in this way is applied to three regulators RG4 generates the 5 V to supply the thermal head of the printer RG5 supplies its motors and RG7 generates the 5 V for the logic circuits 2 6 16 Microcontroller supervisory and reset circuit See schema E330014 sheet n 1 y 3 The microcontroller supervisory and reset circuit is formed by the integrated circuit MAX691A U7 This chip measures the supply voltage and when it is below a certain reset threshold voltage the signals RESET y CCE are active The RESET signal makes the microcontroller start again and the CCE signal joined to the U8A gate protect the RAM against undesired writings during the powering transitions This chip provides also a backup battery switchover P2 U7 is the Vout pin When Vcc is greater than Vbatt and above the reset threshold Vout connects to Vcc When Vcc falls below Vbatt and is below the reset threshold Vout connects to Vbatt When the instrument is powered the battery is always charging through R30 When mains supply falls Vbatt supplies a backup voltage to the RAM and to the timekeeper chip U14 2 27 3 CHECKS
42. exactly as the schemes indicate The manufacturer declines all responsibility due improper handling made without following the above instructions 5 21 Cleaning the lenses a Dismount the lens holder from the optical support and take the lenses away as indicated in section 5 17 b Clean them as indicated in section 5 20 c Mount them again in their holder and the holders in the optical support as indicated in section 5 17 5 22 Cleaning the photodiode a Dismount the photodiode as indicated in section 5 12 b Clean them as indicated in section 5 20 c Mount the photodiode again section 5 12 5 23 Cleaning the aspiration system a It is necessary to clean the sipping circuit properly after each series of measurements and at the end of the day On finishing a series of measurements wash the sipping circuit with abundant distilled water 5 23 At the end of the working day wash thoroughly with a detergent solution such as the one provided with the instrument Lastly rinse with distilled water and empty the circuit by performing wash cycles with air Finally to maximise the life of the peristaltic tubing it is advisable to take it out from its mounting so that it remains loose and without tension On starting a new work session re insert it in its place b If the outside end of the sip tubing has deteriorated a few millimetres may be cut making a perpendicular and clean cut In this case the POSITIONING para
43. f steps is not detected or NOT PASSED otherwise 4 8 Cuvette temperature Allows the accuracy s checking at a given temperature To select this option press the function key CHECK in the UTILITIES SERVICE Password ADJUSTMENTS HEATING menu The cursor appears in the line TEMPERATURE TO CHECK Insert the calibrated digital thermometer s temperature probe into the cuvette holder Input the temperature to be checked and press ENTER The message Heating is displayed with a time counter going down After the five minutes the screen s prompt asks for a new temperature Check the reached temperature in the external thermometer 4 2 4 9 Carry Over This check allows studying the cross contamination that happens in case of consecutive readings of samples that strongly differ in absorbance usually the first with high absorbance and the second with low absorbance The instrument requests Reading filter Stabilization time Sample volume Press F1 to begin readings Insert the baseline and then 5 times the first sample R1 to R5 and 5 times the second R6 to R10 The contamination is calculated according to the formula R6 Contamination 1 x100 H7 R8 R9 R10 Where X av 4 4 10 Photometry 4 10 1 Filter sensitivity This test allows knowing the current generated in the photodiode for a given amount of light as a measurement of the sensit
44. he manufacturer declines all responsibility due improper handling made without following the above instructions 5 14 Temperature sensor replacement See figure 5 2 a Remove the case as indicated in section 5 1 b Remove the temperature sensor 16 See figure 5 7 c Take the support 3 and the separator 2 away backwards d Unsolder the wire from the sensor pins 4 e Solder the new sensor 1 f Move the support 3 forward until the separator 2 contacts the photodiode g Put silicone in the sensor s edge 5 14 h Screw the set again to the cuvette holder I Place the case again as explained in section 5 1 5 15 Lamp replacement This instrument in equipped with a 12 volt 20 watt halogen lamp with an estimated shelf life of 2 000 hours When a change of the lamp is needed proceed as follows see figure 5 11 a Remove the case as indicated in section 5 1 b Wait until the lamp radiator is cold before removing the lamp holder C Loosen the Allen screw 1 of the lamp radiator d Pull back the flange 2 fixing the lamp holder e Remove the lamp holder 4 loosen the Allen screw 3 and remove the lamp f Insert the new lamp 5 fully introducing the pins Tighten the Allen screw 3 until the lamp is tightly secured Do not touch the glass bulb with the fingers to manipulate the lamp the cover itself may be used cutting it by the terminals side and pressing until they come out 9 R
45. he thermal printer is controlled by means of its two own unipolar stepper motors One moves the head and the other the paper feed Eight thermal points form the printer head and a micro switch detects the head s initial position HOME This micro switch has a pull up resistor AR1 and the signal is read by the microcontroller pin P60 66 U13 Both motors and thermal points are controlled by the microcontroller by means of U9 eschema E33001A The motors are controlled using the bus MTR O 7 and the thermal points through the bus PRT O 7 These logic signals are powered by the drivers ULN2803A U1 and U3 The capacitors C12 C13 C15 C17 C24 and C25 are the bypass ones 2 6 11 Display circuit See schema E33003A and E33001A sheet n 1 As a screen it is used a graphic liquid crystal display Hitachi LMG7520RPFC with a CFL backlight Its resolution is 320 x 240 dots The LCD is connected to J2 in the printed circuit I33004A Both control signals and data are sent to the display by U9 according microcontroller control commands The J2 s pin 4 VDD is directly connected to 5V Vcc supply voltage The capacitors C7 and C9 are for bypassing The LCD contrast is controlled by means of a negative adjustable voltage from O to 22V through the VEE P6 J2 line This adjustable voltage Vee is generated by the switching regulator LT1111 U2 The switching regulator output voltage is controlled by the duty cycle of the PWM signal sent b
46. heck the absence of air bubbles inside the cuvette With the cuvette filled with water measure the sensitivity of each filter To do it go to the menu UTILITIES SERVICE TESTS PHOTOMETRIC FILTERS SENSITIVITY Make sure that during the test the cover is closed The reading process for each filter begins A list of sensitivities expressed in nA for each filter is printed Once the list is completed check that the values fall within the ranges appearing in the table of Section II 6 Otherwise check that The inside and outside of the cuvette is clean There are no bubbles inside the cuvette The tubing connections are tight The teflon tube is not strangled anywhere The filter wheel is correctly positioned 3 13 4 CHECK TESTS 4 1 Activation of a test The program includes a set of test programs allowing the performance s verification of diverse parts of the instrument and help in trouble shooting To perform a test follow the path UTILITIES SERVICE Password TESTS The tests available are described in the following sections 4 2 Screen When this test is carried out each time the key ENTER is pressed a different action is done on the screen Check that the actions are uniform and there is no anomalous function of the display 4 3 Buzzer When performing this test the internal beeper sounds several times 4 4 Keyboard Each time a key is pressed the beeper sounds and the key is di
47. ingers Lenses filters and photodiode should be held by their sides lamps by the connecting terminals To clean the components first take dust away using the rubber bulb thus scratches caused by small particles on the surface can be avoided when rubbing with paper n the case of persistent or greasy dirt clean with a paper soaked with the alcohol ether solution and then with a dry paper Sometimes for instance when cleaning the filters or the photodiode window the cotton ear picks may be helpful together with the paper in the most delicate parts After any cleaning it is convenient to repeat blowing with the rubber bulb thus eliminating any residual paper or cotton nap When mounting or dismounting any component take into account the corresponding tools and procedures as they are thought to avoid manipulation problems 5 20 Fig 5 9 Fig 5 11 5 22 5 20 Cleaning the filters a Dismount the filter holders from the wheel and take the filters away as indicated in section 5 16 b Clean them as indicated in section 5 20 c Mount them again in their holders and the holders in the wheel as indicated in section 5 16 Very important In order to meet the electrical safety and electromagnetic compatibility standards the handling instructions given in every section must be strictly followed and it is extremely important to assure that the length of the wires and strips as well as the connections are
48. is strongly recommended 2 3 Thermostatization system 2 3 1 Components Figure 2 9 shows an schema of the thermostatization system that is composed by the following parts a A cuvette holder 1 A temperature sensor 2 An amplifier of the temperature sensor 3 The microcontrollers A D converter 4 A Peltier Cell s in circuit power driver 5 f A Peltier Cell 6 g A heat sink block optical system holder 7 b C d e 2 3 2 System description The cuvette with the reaction mixture to be thermostatized is placed in its holder 1 Thermal contact is settled between the cuvette and the holder The cuvette holder is isolated from the optical system holder 7 and contacts one of the Peltier Cell s faces 6 The other cell s face is in contact with the optical system holder The Peltier Cell pumps heat from one face to the other depending on the current sense The power control circuit 5 is in charge of making that current circulate in the adequate sense inside the Cell in order to heat or cool depending on the microcontroller s instructions When heating heat is pumped from the environment taken from the optical system holder to the cuvette holder and when cooling the opposite is done The optical system holder has a heat sink block to cool the heat coming from the cuvette holder A temperature sensor 2 gives a small voltage directly proportional to the cuvette holder s temperature that is conditi
49. ivity of the instrument for each filter The sensitivity is read for all the filters programmed in the filters table This test can be performed without cuvette sensitivity with air or with cuvette In this last case it is necessary to fill it with water by performing a WASH cycle The sensitivities read should fall within the ranges indicated in the table of Appendix Il Section 4 for sensitivity without cuvette or in the table of Appendix II Section 6 for sensitivity with flow cuvette filled with water 4 10 2 Electric noise This test allows knowing the noise detected by the converter in the signal coming from the logarithmic amplifier Check that the case and cover are closed to avoid entrance of light 4 8 The instrument selects the 340 nm filter and zeroes After approximately 1 minunte the results are displayed They must be within the tolerance ranges of the table of Section 11 5 4 10 3 Stability of the readings This test allows analyzing the stability of a set of repeated readings done with the same sample When beginning the instrument requests the following Reading filter 340 Stabilization time 1 Sample volume 400 Interval time 1 Number of intervals 30 Values between brackets are those recommended for a routine test Program all these parameters depending on the sample The duration of the test will be the result of the interval time and the number of intervals Press F1 to begi
50. meter must be re calibrated c Replace tubing by a new one in case of deterioration Always use original parts 5 24 Cleaning the flow cuvette Cleanness of both the outside and the inside of the flow cuvette is very important Proceed as follows a To clean the inside proceed as described in section 5 24 b To clean the outside use alcohol and then dry with a soft paper section 5 19 5 25 General cleaning of the instrument It is important to avoid dust in the instrument that could affect the optical system Carefully remove dust from the inside of the instrument especially from the fan vanes 5 24 APPENDIX I TECHNICAL SPECIFICATIONS 1 1 Optical system Measurement range 0 to 2 2 A at 340 nm 0 to 3 0 A from 405 nm Spectral range 340 to 900 nm Resolution 0 0001 A Stability 10 005 A in 30 Repeatability CV x 1 2 96 at 505 nm between 0 05 and 0 1 A 20 readings cv lt 0 1 at 505 nm between 1 7 and 2 0 A 20 readings Accuracyr Error at 340 405 and 505 nm 5 at 0 100 A 2 at 1 000 A 2 at 2 000 A 5 at 3 000 A 405 and 505 nm Light source Halogen lamp 20W 12V Detector Silicon photodiode Bandwidth 10nm 2nm 1 2 Thermostatic control Peltier system Temperature range 23 to 40 C Programming steps 1 C Stability 0 2 C Temperature sensor Resolution 10 mV C Output voltage 10 mV x T Kelvin at 25 C 2 98 V at 270 3 10 V 1 3 Printer Type
51. mmended to carry out an annual revision by the Technical Assistance Service The following operations are to done in the same order as follows IV 1 Cleaning General cleaning of the inside section 5 26 1 Filters section 5 21 Lenses section 5 22 Photodiode section 5 23 Flow cuvette section 5 25 IV 2 Change Aspiration tubing Peristaltic tubing Inner waste outlet tubing Outer waste outlet tubing Lamp if blackened IV 3 Review Keyboard Section 4 4 Beeper Section 4 3 Display Section 4 2 Printer Section 4 5 Peristaltic Pump Motor Section 4 7 1 Filters Wheel Motor Section 4 7 2 Filters Sensitivity without cuvette Section 4 10 1 Electronic Noise Section 4 10 2 Reading Stability Section 4 10 3 IV 4 Check Photometric Accuracy Section 4 10 5 Calibration of the Aspiration System Section 3 2 4 Calibration of the Filters Wheel Section 3 2 3 Calibration of the Temperature Section 3 2 2 1 Remove filters lenses and photodiode and proceed with the general cleaning IV 1 APPENDIX V SPARE PARTS AND ACCESSORIES V 1 Accessories Code Description User s Manual AC4460 Paper Lodgment Cover AC964 Paper Roll TB 50 KS 4AO mtp 401 AC3778 Flow Cuvette Hellma 178 712 OS 8 5 mm AC944 Cuvette Hellma 6030 TU2791 Teflon Tubing Hellma 040 203 AC3594 Cuvette Outlet Connector TU1643 Cylindrical Silicone Connector AC348
52. mp probe and press ENTER is displayed Insert the calibrated digital thermometer s temperature probe into the cuvette holder and press ENTER The message Heating is displayed with a time counter going down When the time reaches zero the message Input cuvette temperature at 25 C appears Input the temperature measured by the thermometer corrected and press ENTER The message Heating with a time counter going down is displayed again When the time reaches zero the message Input cuvette temperature at 37 C appears Input the temperature measured by the thermometer corrected and press ENTER 3 4 Press the function key EXIT The program asks if the adjustment is to be saved Answer YES if the results obtained are within the tolerance ranges 3 2 2 4 Explanation of the list parameters STEP Each of the adjustment phases READ TEMPERATURE Theoretical temperature that is read before applying the correction factors CURRENT TEMPERATURE Temperature indicated by the thermometer that must be typped by means of the keyboard ABSOLUTE ERROR Difference in degrees between the read and real temperatures E E T real Tread RELATIVE ERROR Percentage of deviation of the real temperature with regards to the temperature read T rea Tread E reative OFFSET Additive correction factor SLOPE Multiplicative correction factor 3 2 2 4 Option CHECK
53. n The instrument requests the baseline and then the sample If the sample is the same as the baseline the zero stability can be checked For a routine test make the BASELINE with the calibrator n 0 of the calibration kit SERVICE TOOLS code 005 and use calibrator n 4 as the SAMPLE The number of the reading the time and the absorbance are printed At the end the statistical data are printed average maximum and minimum absorbance 4 10 4 Precision This test performs successive readings of different samples of the same liquid repetitivity between identical samples The instrument requests Reading filter Stabilization time Sample volume Number of intervals Press F1 to begin the test 4 4 The instrument request the baseline and then as many samples as intervals programmed At the end the statistical data are printed average coefficient of variation maximum and minimum absorbance 4 10 5 Accuracy This test allows checking the accuracy when reading a sample of know absorbance WARNING The reliability of this test requires a sample absorbance value determined precisely with a reference method When beginning the test the instrument requests Reading filter Stabilization time Sample volume Press F1 to begin the tst Insert the baseline Then the instrument asks for the reference value Insert the theoretical value by means of the numeric keyboard Insert the sample when requested
54. o an interference filter 4 This is one of the nine possible ones located in the wheel One stepper motor drives this wheel and controlled by the program place the proper filter in the optical axis in front of the light beam Each filter monochromes one wavelength with the characteristics described in section l 1 A second lens 5 focuses the monochromatic beam in the cuvette center 6 In case of flow cuvettes the light path hole itself behaves as a diaphragm decreasing the sensitivity that it is lower than in the common cuvettes The 2 1 light beam goes through the cuvette as far as a light sensor 7 that converts the light beam into electric current 2 1 3 Physical description The optical system figure 2 2 consists in an injected aluminum holder 1 where there are mounted the following components A heat sink shape aluminium block 2 containing a lamp holder 3 That block includes a diaphragm figure 2 3 1 that delimits the a light solid angle A lens holder 4 that contains the first lens A filters wheel 5 with capacity for 9 filters This filters must be mounted in special holders 6 This wheel is moved through a belt 8 by a stepper motor 7 A photointerrupter 9 allows the detection of a stem 10 that indicates the filters wheel home position A lens holder 11 that contains the second lens A light protector 12 fitted together the second lens holder avoids the parasite light int
55. oned by one amplifier 3 and read by the microcontrollers A D converter 4 The microcontrollers thermostatization program depending on the programmed temperature and the read value activates the power control 5 heating or cooling as required 2 9 Fig 2 6 Fig 2 7 2 11 Fig 2 8 Fig 2 9 2 13 2 3 3 Physical description The cuvette holder 13 figure 2 2 is fixed to the optical system holder 1 by means of four screws 14 thermally isolated The Peltier Cell 15 is located between the cuvette holder 13 and the optical system holder 1 The temperature sensor located in a plastic holder 16 is fixed to the cuvette holder by a thread 2 3 4 Programming Programming the thermostatization system consists in indicating the instrument which temperature is desired for the reaction mixture This value may introduced in several program s points 2 3 5 Adjustment Components tolerance produce a deviation between the programmed temperature and the real one so it is necessary an adjustment procedure to compensate such deviations The thermostatization adjustment procedure is described in section 3 3 2 3 6 Precautions and maintenance The thermostatization system has two critical points the good thermal contact between both faces of the Peltier Cell with the optical system holder and the cuvette holder and the good
56. ot work properly e The concentration results in the multistandard decreasing mode appear in negative e While doing kinetic tests in some cases there are unexpected jumps in the absorbance results Version 1 1 In this version the errors reported for the previous version are solved except the jumps in the kinetics Errors reported e The peristaltic pump s led does not work when doing a base line in tests with temperature Version 1 2 This version solves the problem with the peristaltic pump s led reported in the last version Errors reported e Error when calibrating the peristaltic pump in Portuguese Version 1 3 This version solves the unexpected jumps in the kinetic tests Errors reported e Error when calibrating the peristaltic pump in Portuguese VII 1 Version 2 0 e Chinese language included e New kinetic points editing mode added Warnings e The internal calibration s data structure has been changed It is necessary to calibrate the BTS 330 when upgrading to this new version from lower ones e Now two EPROM s are needed to load the software Errors reported e Error when calibrating the peristaltic pump in Portuguese e Alignment centring and length errors in texts of some languages French Russian Portuguese and Bulgarian Version 2 1 e All the above mistakes are solved Warnings e t is necessary to calibrate the BTS 330 when upgrading to this new version from versions 1 x E
57. power socket and the checkpoint TP4 in the main board and check that the resistance is lower than 0 1 ohm Disconnect the connector J4 of the main board transformer and plug the instrument Using a voltmeter read the alternating voltages in the transformer windings and check that they are within the tolerance limits in the table of Section 11 4 Unplug the instrument and connect J4 Plug the instrument again 3 4 Fan checking a Check that the airflow is outward propelled b Take a measurement of the voltage in the connector J8 positive pin 1 negative pin 3 and check that it is into the values showed in section 11 8 If they are not correct look at the monocard heat sink silicone and the properly fan condition 3 5 Optical system checking If the optical system has been disassembled in order to replace one of its parts lens Peltier cell etc or by any other cause once assembled again the alignment must be checked a b O I Take the case away Take the photodiode away from its lodgement Insert the light beam centering checking tool into the cuvette holder The screen side must be faced towards the lamp Switch the instrument on Look at the tool screen through the photodiode lodgement Check that the light spot is into the outer circle the inner circle simulates the 1 5 mm light pass in the flow cuvette If the step f is correct it means that the reading group is centered then j
58. r supply 2 5 16 Reset and battery backup supervisory circuit 2 6 Electronic circuit description 2 6 1 Logarithmic amplifier 2 6 2 Analog to digital converter 2 6 3 Lamp control 2 6 4 Filter wheel detector 2 6 5 Filters wheel motor control 2 6 6 Temperature sensor amplifier 2 6 7 Peltier Cell control 2 6 8 Pump motor control 2 6 9 Keyboard circuit 2 6 10 Printer control 2 6 11 Display circuit 2 6 12 RS 232 channel circuit 2 6 13 Fan control 2 6 14 Microcontroller 2 6 15 Power supply 2 6 16 Microcontroller supervisory and reset circuit 3 CHECKS AND ADJUSTMENTS 3 1 Service menu 3 1 1 Option TEST 3 1 2 Option ADJUSTMENTS 3 1 3 Option UNLOCK LOCK TESTS 3 1 4 Option UNLOCK LOCK instrument 3 2 ADJUSTMENTS 3 2 1 Photometric adjustment 3 2 1 1 Materials needed 3 2 1 2 General remarks 3 2 1 3 Procedure 3 2 2 Thermostatic system adjustement 3 2 2 1 Materials needed 3 2 2 2 General remarks 3 2 2 3 Procedure 3 2 2 4 Explanation of the list parameters 3 2 2 4 Check 3 2 3 Filters wheel adjustment 3 2 3 1 Materials needed 3 2 3 2 General remarks 3 2 3 3 Manual mode 3 2 3 4 Automatic mode 3 2 4 Peristaltic pump adjustment 3 2 4 1 Materials needed 3 2 4 2 General remarks 3 2 4 3 Check method 3 2 4 4 Manual mode 2 20 2 20 2 20 2 20 2 22 2 22 2 22 2 23 2 23 2 23 2 24 2 24 2 25 2 25 2 26 2 26 2 26 2 27 2 27 3 1 3 1 3 2 3 2 3 2 3 2 3 2 3
59. refully at the bottom of the tube and proceed as requested Press the function key POSITION The message Insert tube with water and press PUMP is displayed 3 10 Proceed as requested The pump sips the water and a few seconds later the following message appears Remove the tube and press ENTER Proceed as requested The pump works and the instrument calculates the positioning During this process the instrument performs photometric readings and therefore the cover must be closed The whole process takes 70 seconds Press the function key CHECK Input 2000 as sample volume 2 mL Perform an aspiration cycle with the volume adjustment tool filled with water until the upper mark 3 mL and check that a The sample tail that has not entered the cuvette is 0 10 mm long c The level of water remaining in the tool is between the two lower marks equivalent to 1 mL tolerance Press EXIT and answer YES or NO to save the adjustment parameters or not Press ENTER Press EXIT again to go back to the main menu 3 3 Transformer and power supply checking Before connecting the instrument to the mains a d Check that the voltage selector is that of the supply voltage rear part of the instrument If not select the correct one by slipping the selector with the help of a screw driver Check the ground connection With the mains wire unplugged connect the ohmmeter between the ground connection terminal in the
60. rom 100 to 4 000 uL Flow cuvette 18 uL Carry over see table l 1 SAMPLE CARRY OVER VOLUME 96 2 25 2 70 0 75 1 50 0 05 0 25 Table l 1 1 11 Environmental conditions Indoor use Temperature 15 C to 35 C Maximum relative humidity 75 Pollution degree 2 APPENDIX Il ADJUSTMENT TOLERANCES TABLES 11 1 Photometric adjustment tolerances Filter Calibrator Relative error tolerance 340 1 7 0 5 0 2 6 0 5 0 3 5 0 5 0 4 5 0 5 0 405 1 7 0 5 0 2 6 0 5 0 3 5 0 5 0 4 5 0 5 0 505 1 7 0 5 0 2 6 0 5 0 3 5 0 5 0 4 5 0 5 0 11 2 Filters wheel adjustment Steps number without adjustment to put the first filter 208 Adjustment steps Maximum 40 Minimum 20 Minimum sensitivity at 340 nm 30 nA 11 3 AC voltage in the Transformer Secondaries VOLTAGE MINIMUM VALUE MAXIMUM VALUE V V Il 1 11 4 Filters sensitivity without flow cuvette FILTER nm MINIMUM VALUE MAXIMUM VALUE nA nA 340 50 250 405 200 1500 420 400 2000 450 1000 3500 492 1500 5500 505 1500 8000 530 2000 9000 546 3000 1100 578 4000 16500 600 5000 17500 630 6000 20000 670 7000 26000 11 5 Electric Noise PARAMETER MINIMUM VALUE MAXIMUM VALUE AVERAGE VALUE 400 mV 400 mV NORMAL VALUE BIAS 0 mV 2 mV MAXIMUM PEAK 0 mV 2 mV MINIMUM PEAK 2 mV 0 mV 11 6 Filters sensitivity with flow cuvette FILTER nm MINIMUM VALUE MAXIMUM VALUE nA nA 340
61. rrors reported e When programming the 96 symbol as a unit it turns to when saving e Data alignment error in the printer test report e There are errors when typing the serial number Version 2 2 e All the above mistakes are solved Warnings e t is necessary to calibrate the BTS 330 when upgrading to this new version from versions 1 x Errors reported e The calibrator absorbance in the fixed time mode is printed with a negative symbol VII 2 Version 2 3 The next improvements have been added e Korean language is added e Czech language is added e n the kinetic fixed time and multiple tests the initial absorbance Ao is printed e Every time that a test is started the sample number is reset to 1 e The instrument doesn t begin to read absorbances until the printer doesn t finish to print the header report This version solves the next reported errors e The mistake about printed negative symbols in the calibrator s absorbance in fixed time tests is solved e n any rare circumstances the printer could stop the timer and the test was blocked This version solves this problem Version 2 4 The next improvements have been added e Included a new calculus mode diferential mode with multicalibrator e n kinetic mode will show the delta values e Yugoslavian language is included This version solves the next reported errors e The configuration and quality control data will save in flash memory
62. s the function key EDIT VALUES and then input all the standard values for each filter When the last value for each filter is input the display automatically changes to the next filter When the last filter is completed the program exits the Edit mode the Edit mode can be left at any time by pressing the key EXIT In case of mistake while entering the values press C to delete the value and key it in again Proceed as follows to correct previously validated values Exit the Edit mode by the function key EXIT taking care of previously validating with ENTER the last value Enter again in the Edit mode if the erroneous value is still on the screen Otherwise use the cursor up and down keys until the desired values appear on the screen Press ENTER until the cursor is located on the standard to be edited Another option is to enter in the Edit mode with the first standard of the first filter and to press repeatedly ENTER until the cursor is located on the standard to be edited Press the function key ADJUST The program will request the standard values to be introduced Once the standards read an automatic printing process starts in which the following data appear for each wavelength and standard the absorbance read the expected absorbance the relative and absolute errors The tolerances appear in Table Il 1 of Appendix Il Press the function key EXIT The program asks if the adjustment is to be saved Answer YES if the relati
63. splayed Press EXIT twice to exit 4 5 Printer A set of characters is printed and then 10 rows of asterisks with the 10 programmable intensities Check that the characters listed are clear and legible and that the scale of intensities is growing and regular 4 6 Serial Port RS 232 This test sends the key being pressed by the line TxD pin 5 of the COM1 connector Fig 2 7 and should be received by the line RxD pin 3 of the COM connector To do this it is necessary to make a bridge between these lines Each time a key is pressed the corresponding character is showed in the section of the display corresponding to the characters sent and the same character should appear in the section of characters received Exit by pressing 4 1 F5 From this test it is possible to modify the communication configuration parameters 4 7 Motors This menu allows checking two motors the peristaltic pump motor and the filters wheel motor 4 7 1 Loss of steps of the peristaltic pump Proceed as follows a Turn the pump rotor by hand until the arrow is in front of that of its support b Press ENTER The pump operates for some seconds C Check that the rotor turns without eccentricities or abnormal noises d When stopped again check that the two arrows keep the same original position 4 7 2 Loss of steps of the filters wheel The filters wheel performs several movements Afterwards the instrument displays PASSED if a loss o
64. t supplies the regulated 12V that the lamp needs to work properly 2 5 4 Filters wheel detector It is a photointerrupter to detect the filters wheel home position Allows the microcontroller to know the location of each filter thus being able to position them in front of the light beam 2 5 5 Filters wheel motor control This is a circuit that by means of the microcontroller s logic control supplies power to the stepper motor that moves the filters wheel 2 5 6 Temperature sensor amplifier This is a circuit to make the signal conditioning for the temperature sensor s voltage level in order to be measured by the microcontroller s 10 bits analog to digital converter with a resolution enough to adjust the system 2 5 7 Peltier cell control This is a microcontroller controlled power circuit that supplies the Peltier cell the adequate current to heat or cool the cell holder 2 18 2 5 8 Pump motor control This is a circuit that by means of the microcontroller s logic control supplies the needed power to the stepper motor that moves the peristaltic pump 2 5 9 Keyboard circuit This circuit basically consists in the keyboard itself and some protection diodes 2 5 10 Printer control It consists in the power circuits that allow the orders coming from the microcontroller to act on the printer either moving the motors or heating the thermal head points 2 5 11 Display circuit This circuit consists in the display itself the
65. timate and should be determined by error and trial using the function CHECK of the menu ADJUST PUMP PUMP DELAY It corresponds to the elapsed time between the aspiration cycle and the positioning into the cuvette Input the number of seconds recommended 2 and press ENTER The manual adjustment is completed To check it use the volume adjustment tool filled with water until the upper mark 3 mL Press the function key CHECK Input 2000 2 mL as sample volume Perform an aspiration cycle with the volume adjustment tool and check that a The sample tail that has not entered the cuvette is 0 10 mm long b The level of water remaining in the tool is between the two lower marks equivalent to 1 mL tolerance Press EXIT and answer YES or NO to save the adjustment parameters or not Press ENTER Press EXIT again to go back to the main menu 3 2 4 5 Automatic mode Insert the flow thru cuvette in its holder making sure that its position is correct Press the function key AUTOMATIC Press the function key SAMPLE VOLUME The message Put a tube with 5 mL water and press PUMP is displayed Pipet precisely 5 mL distilled water into an assay tube and proceed as requested taking special care that the end of the sipping tubing reaches the bottom of the assay tube Approximately 4 mL are sipped at normal speed and then continues slowly The following message appears After sipping the last drop press ENTER Look ca
66. ube to the outlet 8 in the lower part of the tray 5 9 5 8 Filters wheel replacement To manipulate the filters wheel proceed very carefully in order to avoid scratching or soiling the filters It is strongly recommeded to remove the filters holders from the wheel previously Proceed as follows see figure 2 2 a Remove the cuvette holder tray as described in section 5 7 b Loosen the Allen screw 17 c Remove the shaft 18 d Remove the wheel 5 To place the wheel again proceed as follows a Put the wheel 5 in its place taking into account to position the belt 8 and the two washer s 19 as indicated in the figure b Place the shaft 18 C Tighten the screw 17 If the filter holders were removed place them again in the same order as they were If order is changed the filters table must be re programmed 5 9 Filters wheel motor replacement See figure 5 5 a Remove the cuvette holder tray as indicated in section 5 7 b Disconnect the strip of the motor from the main board J6 O Remove the screws 1 2 Change the motor 2 placing the belt 3 as indicated in the figure Place the motor and fix the screws 1 2 f The screw lodgings have a little slackness to allow stretching the belt Avoid excess stretching 5 10 5 10 Peristaltic pump replacement See figure 5 2 a Disconnect the peristaltic pump motor strip J3 from the main board b Remove the 4 screws 5
67. ufacturer with next default parameters Baud rate 9600 Timeout 0 Terminal number O 2 4 3 Information transmitted The communications procedure uses a sending amp waiting protocol Every time the transmitter sends a message it waits for an ok from the receiver If the ok message is not received after a programmable timeout period the message is sended again The CRC 16 error detection code is used to check the received messages The communications procedure works using a MASTER SLAVE method so it is possible to communicate several instruments at one time To achieve this every instrument has to be configured with a different identification terminal number This parameter is configured in the CONFIGURATION COMMUNICATIONS menu Communications are always started by the MASTER the computer The SLAVE the instrument is in a hearing mode and only sends information when it is required by the MASTER 2 15 The instrument can send the information below Quality control values Concentration values In order to get the communication between the computer and the instrument a proper computer running software is needed This program save the data received from the instrument into the computer hard disk These data are stored in an EXCEL compatible format The file format used is showed in figure 2 11 2 4 4 Programming In order to get a suitable communication with a computer through the serial
68. ump to the step n else continue from the next step Remove the lamp holder set Take note of its current position According to the point of view of deepness and inclination check if the lamp is properly positioned In this case place it again rotating it 180 degrees and check the light spot again see step f If there were problems with the lamp holder set fix them up place it again in the last position and check again the light spot see step f If the problems persist change the lamp position and jump to step f If the problems persist in spite of all the above handling change the lamp for a new one and jump to step f Place the photodiode in its lodgement again taking care of its cleanness m Close the instrument 3 12 n At the end a functional checking of the optical axis light beam centering is done by a sensitivity test with a flow cuvette with distilled water or a macro cuvette with distilled water 3 6 Check of the sensitivity with flow cuvette a Switch the instrument on b Clean the external faces of the cuvette with a mixture of ethylic alcohol and O ether then dry it with a soft paper Section 2 1 6 Insert the flow cuvette and its connections Wash the cuvette with 1 mL washing solution and then with abundant distilled water by means of the WASH key Make sure that the instrument is on for at least 20 minutes Fill the cuvette with distilled water by means of the WASH key C
69. ve errors obtained are within the tolerance ranges 3 3 If there is no accomplishment with the tolerance ranges repeat the procedure in order to discard a mistake due to factors like errors during the procedure in the calibrators and or instability of the electric supply Once all these external factors discarded if the instrument does not accomplish the ranges and presents abnormal values in the upper part or the absorbance range check the photodiode wire and the welding in the analog part of the printed circuit board 3 2 2 Thermostatic system adjustment This procedure describes the way to adjust the thermostatic circuit of the cuvette holder Both the cuvette holder temperature sensor and its associated electronic circuit have some tolerances that must be compensated in order to have the cuvette temperature as programmed The adjustment allows using a thermometer the calculation of the correction coefficients needed to perform this compensation 3 2 2 1 Materials needed Calibrated digital thermometer and temperature probe 3 2 2 2 General remarks Switch the instrument on at least 20 minutes before beginning the adjustment It is very important to take into account the corrections indicated in the digital thermometer when registering the temperatures 3 2 2 3 Procedure Enter in the UTILITIES SERVICE Password ADJUSTMENTS HEATING menu Press the function key ADJUSTMENTS The message Insert the te
70. voltage dividers of these circuits C13 and C36 are bypass capacitors for the 5 V supply voltage of these circuits The other connected components complete its operation 2 6 6 Temperature sensor amplifier See schema E330014 sheet n 1 The temperature sensor generates a voltage proportional to the temperature expressed in Kelvin degrees Celsius 273 equal to 0 01 V K So for instance at 25 C VT 254273 x 0 01 2 98 V at 37 C VT 37 273 x 0 01 3 10 V The U20 amplifier and its associated circuit make these voltages adequate to be read with the maximum resolution by the 10 bit A D converter of the Hitachi H8 3003 microcontroller The adequate voltage exits by 6 U20 and it is applied to the microcontroller s input ANO 86 U13 through the resistor R47 As the voltage coming from 6 U20 can vary in the range 15 V and the input voltage of 86 U13 cannot exceed the converter reference voltage applied to AVCC 84 U13 5 V the circuit formed by D11 D13 D14 D15 and R50 is in charge of keeping this input between this value and ground 2 6 7 Peltier Cell control See schema E330014 sheets n 1 and 2 A low voltage full wave rectified voltage is applied to the Peltier Cell in the sense adequate for heating or cooling Although the use of this current lowers the performance of the cell its use is justified by the simplicity of the circuit The full wave rectification in both senses is achieved using two triacs and a
71. y the microcontroller P4 U13 PWM 1 3 KHz 0 Duty Cycle OV 10096 Duty Cycle 25V The LCD backlight supply voltage is connected to J8 The backlight supply voltage is generated from Vpm 5V by the inverter circuit see the block in wich the TRF1 transformer is 2 25 Look out The backlight supply voltage J8 is up to 300Vrms 85 KHz and 6mArms The lamp start voltage is 1000V minimum 2 6 12 RS 232 channel circuit See schema E330014 sheet n 1 The serial communication is achieved by one of the two ACIA integrated in the microcontroller It supplies all the necessary functions and the communication uses two lines TxD2 transmitter data 20 U13 and RxD receiver data 22 U13 The lines logic level is TTL The signal conditioning to the RS 232 C E l A standard is made by the integrated circuit MAX202CSE U17 and a set of associated capacitors They generate the needed positive and negative voltages The communication lines enter and exit through the J15 connector Figure 2 10 2 6 13 Fan control See schema E33001A sheet n 2 and 3 The fan s power supply is done by an independent circuit formed by the 24 V transformer coiling the rectifier bridge D6 and the capacitors C38 and C52 that generate a non regulated voltage The voltage to apply to the fan is controlled by the regulator RG6 The output voltage is given by the voltage divider formed by R29 and NTC1 wich is in contact whith the power supply heat sink

SERVICE MANUAL - Frank`s Hospital Workshop

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