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HP1047A RID

1. Figure 23 Recorder Control Electronics Refer to REMOTE CONTROL FUNCTION ELECTRONICS The MARKER indicator LED is ON for the duration of the offset Control Board eis Board ae Recorder a scorder Contro Output Circuits 5 Circuits O Con 105 Con 105 Con 115 Con 1101 Key Con 601 Switch Range Board Select so oO oO Switeh Polarity Recorder Marker Range Switch Board ae Con 602 O O O Polarity Recorder Marker LED LED LED LED Board 1 54 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Summary of Switches and Potentiometers There are many potentiometers throughout the detector boards Some of these are used during normal service calibrations but many are not These are pre set at the factory and should not be misadjusted The following information board by board is meant to provide information on potentiometers switches and certain test points This information may be used in the event that a user has misadjusted a potentiometer or changed a switch position If for some reason a particular circuit cannot be re adjusted correctly anew board must be ordered Any test points which are not mentioned in this manual are used by the factory in testing and have no practical value in board l
2. 3 Replace the KEY SWITCH board If no Is the flush command applying 12 VDC as needed to the drive wires of the valve If yes replace the valve If no 1 Deactivate the valve from the rear panel then check for correct front panel operation 2 Replace the CONTROL board 3 Replace the POWER SUPPLY board Service Handbook for 1047 Refractive Index Detector 12 2001 117 Troubleshooting Information Table 11 Flush Valve Problems Possible Problem Actions Valve is stuck in the activated position The manifestation Is the 12 VDC being continuously applied to the drive of this problem will be that each movement on the wires of the valve chromatogram will now have a counterpart of the opposite polarity For example a negative solvent front will be followed by a large positive offset and all positive peaks will be immediately followed by negative peaks Note that the negative peaks will be broad due to the large post sample cell volume of the detector In the case of a chromatogram with peaks close together it will be difficult to determine that this is what is actually happening If no replace the valve If yes 1 Try to deactivate the valve from the rear panel then from the front panel 2 Replace the CONTROL board 3 Replace the POWER SUPPLY board 118 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Table 12 Troubleshooting Information Temperature
3. Has the photo diode assembly been replaced recently It is possible that the new photo diode assembly requires a re adjustment of the mirror Refer to Mirror Adjustment on page 85 for the mirror adjustment procedure Replace the detector left side cover and reconnect the tubes of the flush valve If the flush valve is incorrectly plumbed the detector flow cell may be destroyed Make sure the flush valve is plumbed according to Figure 29 on page 71 10 It is suggested to verify the program of electrical calibrations described in Electrical Calibrations on page 93 76 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Procedures Power Supply Component Replacement Refer to Figure 43 on page 138 With the exception of the main power transformer and the POWER SUPPL Y board all the components of the power supply are shown here Refer to Figure 12 on page 37 Using this wiring diagram replace the defective component If you are replacing an old style rotary voltage selector with the current slide type re wire as shown in Figure 12 on page 37 Service Handbook for 1047 Refractive Index Detector 12 2001 77 Procedures Optical Unit Replacement The replacement optical unit is available either with AB Motor assembly or without AB Motor assembly This procedure assumes that the replacement optical unit DOES NOT include the AB motor assembly 1 Refer to Figure 41 on page 134
4. Lens Assembly Screws a 7 Mirror Adjust Glass Access Hole se 8 Mirror Adjust Glass 9 Optical Balance Glass 10 Lamp Holding Collar Set Screw 1 5 mm HEX 11 Lamp Assembly 12 Lamp Holding Collar lt F PEN 2 8 as A SY 12 d 10 Service Handbook for 1047 Refractive Index Detector 12 2001 81 Procedures Figure 33 Light on Slit 6 When the lamp is correctly adjusted carefully tighten the holding collar so that there is no danger of the lamp changing position Tighten the set screw in the collar 7 Replace the optical unit insulation foam and top covers If all else is correct mirror adjustment ok no air in flow cell etc display TP1 2 can be used to determine the light intensity The average light intensity TP1 and TP2 both pressed MUST be between 4 5 and 6 0 8 Perform the complete program of electrical calibrations described in Electrical Calibrations on page 93 NOTE It sometimes happens that a new lamp assembly cannot be correctly adjusted This is due to the fact that the orientation of the bulb in the bulb holder does not provide even one position where correct adjustment can be achieved The bulb is positioned in the holder by three set screws Loosen two of these screws and introduce a small change in the orientation of the bul
5. M3 6 mm long 0515 0924 Lock washer 2190 0108 Flat washer 3050 0891 24 Nut M3 0535 0025 Lock washer 2190 0108 25 Standoff 7 mm OD x 8 mm height 0380 0005 26 Lock washer int tooth 10 5 mm ID 18 mm OD no p n 136 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Front Panel Assemblies Figure 42 137 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Rear Panel Assembly The following table identifies the individual parts of the Rear Panel Assembly as shown in Figure 43 Figure 43 Rear Panel Assemblies Table 17 Rear Panel Assembly Description ERC number Part number 1 Ribbon Cable 4 from Output Board CON703 to 01047 61606 Motherboard CON114 2 Output Board includes two 9 pin terminal strips ERC75007B 01047 66515 not shown 3 Output Board Holding Clamp 01047 86103 4 Response Time Switch Assembly 01047 60006 5 Ground Terminal Cable Assembly no p n 6 Rear Panel no p n 138 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Table 17 Rear Panel Assembly Description ERC number Part number 7 Fuse Holder Assembly 01047 42301 8 Voltage Selector 01047 81902 g Power Socket 01047 85201 10 Temperature Power Switch 01047 81901 11 Heat Sink 01047 81101 12 Ground Terminal 01047 86101 13 Power Regulator Set 01047 42401 14 Power Regulator Insulator 0104
6. Remove the detector top cover Remove the left side cover item 15 by removing screws item 4 Disconnect the tubes from the IN OUT block item 17 so that the side cover can be completely removed Remove the right side cover item 3 also by removing screws item 4 2 Again referring to Figure 41 on page 134 remove the Preamplifier board safety cover item 34 Carefully un solder from the preamplifier board the three wires white green clear of the photo diode assembly cable This cable can be seen coming from the front of the optical unit beneath the lamp Make a note of the position of each wire before un soldering Disconnect the AB motor assembly cable 4 Disconnect the lamp wires from their positions on the VALVE LAMP terminal block 5 Disconnect the remaining flow cell capillary tubes one from the three way fitting and two from the flush valve 6 At the rear of the optical unit disconnect the leak drain tubing from the optical unit leak drain fitting 7 Atthe Temp and Motor Control board disconnect the optical unit heater wires from CON201 and the optical unit temp sensor from CON203 8 Carefully raise the detector to gain access to the optical unit holding screws under the detector Again refer to Figure 41 on page 134 Completely remove both of the locking screws item 32 Then carefully remove the four nuts which secure the optical unit support feet item 7 9 Carefully pull the optical unit free of the detecto
7. SENSOR about 5 2 KQ cold about 2 8 KQ at 40 C Overall circuit performance is best determined by measuring HEATER voltage power directly at CON201 of the TEMP AND MOTOR CONTROL board and by observing the state of the front panel temperature indicator LED e TEMP SWITCH off 0 V AC with indicator LED off e While heating up to setpoint full power about 100 V AC indicator LED solidly on e While regulating at the setpoint about 1 2 power indicator LED flashing e Actual temperature greater than setpoint nearly 0 V AC indicator LED off Service Handbook for 1047 Refractive Index Detector 12 2001 43 Hardware Information Figure 16 Heater Control Electronics Rear Panel Temp ON OFF Switch OR Optical Unit ne U oe OR Heater lemp Temp E from e WHT Fuse Sensor LEG Transformer 1OOVAC GRAY PINK 2x secondary as a BRN BRN i CON 113 Fl CON 202 CON 201 z a Temperature 3 YD of OAMP Regulator aed Front Panel e IP201 TP203 Temp Select nar SS Rog Driver 108 cto Z Temp nN Switch S Control X Zz oO OF Mother Temperature and Motor Control Board Board 44 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information A broken connection from either the TEMPERATURE SELECT SWITC
8. The WARNING sign denotes a hazard It calls attention to a procedure practice or the like which if not correctly performed or adhered to could result in injury or loss of life Do not proceed beyond a WARNING sign until the indicated conditions are fully understood and met Service Handbook for 1047 Refractive Index Detector 12 2001 11 CAUTION WARNING WARNING WARNING WARNING WARNING General Information The CA UTION sign denotes a hazard It calls attention to an operating procedure practice or the like which if not correctly performed or adhered to could result in damage to or destruction of part or all of the equipment Do not proceed beyond a WARNING sign until the indicated conditions are fully understood and met 1047A related warnings Always take care that the LC system does not have excessive restriction downstream of the 1047A Downstream restriction of greater than 2 bar 29 psi will damage the 1047A Always disconnect the line power cord before removing the top cover of the detector Line power should also be disconnected before removing replacing any electrical component Certain alignment procedures require that the top cover be off while power is connected to the instrument Beware of the hazardous voltages that exist Always observe proper anti static precautions Solvents or samples used in detector applications may be very toxic and or acidic Take sp
9. information about the Agilent 1047A refractive index detector This manual is available as electronic version Adobe Acrobat Reader file only
10. removed Remove the eight screws indicated in the figure Carefully pull the front panel assembly forward to gain easy access to the front panel components It will probably be more convenient to disconnect the flush valve wires from the VALVE LAMP terminal block Top view with front cover removed or Zs 68 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Figure 28 identifies the various components of the front panel assembly The figure also indicates those screws which secure a board and provides cabling information Figure 28 Front Panel Assembly Table 4 Legend Front Panel Assembly Term Description Term Description 1 Digital Display Board 12 Integrator Adjust Assembly 2 Key Switch Board 13 LED Board 2 3 Ribbon Cable to Motherboard CON115 14 Ribbon Cable to Motherboard CON109 4 Holding Screws for LED Board 1 15 Holding Screws for LED Board 2 5 LED Board 1 16 Display Switch Board 6 Flush Valve 17 Ribbon Cable to Motherboard CON111 7 Temperature Select Switch Assembly 18 Holding Screws for Digi
11. 0100 0900 The inlet capillary tube MUST be plumbed as shown Failure to do so will rupture the flow cell The inlet capillary has an ID of 0 25 mm All other tubes have an ID of 0 8 mm The inlet capillary is usually on the bottom as shown but always check the ID of the tube to be certain The flow cell has a back pressure rating of only 7 bar C Both the Flush Valve and the 3 Way Fitting can accept SWAGELOK nuts and ferrules Swage depth is 3 5 mm D Internally the 3 Way Fitting is a T Internal diameters are large to minimize restriction DO NOT substitute a zero dead volume T for the 3 Way Fitting E This is a WIDE ID 1 16 inch union DO NOT substitute a standard zero dead volume union for this part excessive restriction might rupture the Flush Valve or the Flow Cell F The Flush Valve has a back pressure rating of only 2 bar 72 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures L _L_L_L_L____E AB Motor Assembly Replacement and Adjustment Replacement e Remove the detector top cover Refer to Figure 41 on page 134 and remove the left side cover item 15 by removing screws item 4 Disconnect the tubes attached to the IN OUT block e Figure 30 and Figure 31 on page 74 identify the important details of the AB Motor assembly Figure 30 AB Motor Assembly Screws Remove these screws only 4 Refer to Figure
12. 30 Proceed as follows Disconnect the AB motor assembly cable 6 Remove the four screws marked which hold the AB motor assembly to the Optical Unit Pull the AB motor assembly away from the Optical Unit and out of the detector Service Handbook for 1047 Refractive Index Detector 12 2001 73 Procedures 7 With power still off re connect the cable of the replacement AB motor assembly DO NOT yet connect the replacement AB motor assembly to the optical unit Instead place the motor assembly outside the detector in such away that the motor will be able to turn freely and safely Figure 31 AB Motor Assembly Parts ioe e_ _ i TA oo m JH gt of Legend A Optical Balance Glass Control Shaft B Sleeve Coupling C Manual Shaft Turning Knob 74 Service Handbook for 1047 Refractive Index Detector 12 2001 To check for the correct relationship between the AB motor and the optical balance glass proceed as follows Procedures Adjustment A Turn on
13. 3394 6 Verifying the Wander Specification Wander Specification 1 x 10 8 RIU equal to 2 x 10 8 RIU equivalent to 20 x 10 RIU 1 For HP 3390 2 3 at ATTENUATION 0 20x 10 RIU Wander E F O a 4 2 x10 RIU mm Allowable Wander approx 5 mm for HP 3390 2 3 2 For HP 3394 6 at ATTENUATION 0 20x 10 RIU Wander Be Fp aac Nit T 2 1 x 10 RIU mm Allowable Wander approx 10 mm for HP 3394 6 104 Service Handbook for 1047 Refractive Index Detector 12 2001 The Range Dial and Recorder Outputs The Recorder Full Scale Deflection Setting NOTE Performance Verification For the Recorder Outputs Verifying 1047A specifications using recorder outputs is a question of converting the specifications noise drift wander to an allowable RECORDER DEFLECTION at a particular 1047A RANGE setting For this it is necessary to understand the relationship between the 1047A RECORDER OUTPUTS 1 mV and 10 mV the 1047A RANGE dial and the full scale deflection setting of the recorder Assume that the HP 1047A RANGE dial is set to 2 x 10 RIU At this RANGE setting 2 x 10 5 RIU of detector response will produce a signal of 10 mV at the 10 mV RECORDER OUTPUT and a signal of 1 mV at the 1 mV RECORDER OUTPUT The least sensitive RANGE setting is 10 mV and 1 mV of signal output for 32 x 10 5 RIU increment of detector response The most sensitive RANGE setting is 10 mV and 1 mV of signal output
14. 3A Integrator 01040 60105 HP 3394 6A B Integrator or HP 35900A B C 35900 60630 A D Converter Universal strip chart recorders usually require this 01037 60105 Unique 1047A Cables Table 20 Unique 1047A Cables Figure Description Part number Figure 45 From 1090 LC EVENTS to 1047A inputs 01047 60202 Figure 46 From 1047A Balance NR output to 1090 LC 01047 60201 Remote Control Port Service Handbook for 1047 Refractive Index Detector 12 2001 143 Parts Information Figure 45 Cable from 1090 LC ee oo ae oe a RG o a eo eee Se 5 Se SSS r le Mi E Figure 46 Cable from 1047A NR output to 1090 LC BLK RED 144 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Inlet Outlet Tubes Table 21 Inlet Outlet Tubes Description Part number Insulation for inlet tube no p n Inlet Capillary Assembly 1 16 0 D 88 cm by 01040 87602 0 12 mm I D Inlet Capillary Assembly 1 16 0 D 88 cm by 01047 87301 0 25 mm I D Outlet Tubing Assembly 1 8 wide bore teflon 01040 67602 tube plus 1 16 SST adapter fitting Service Handbook for 1047 Refractive Index Detector 12 2001 145 Parts Information 146 Service Handbook for 1047 Refractive Index Detector 12 2001 oe Agilent Technologies In This Book This manual contains technical
15. Amplifier Board Figure 24 is a simplified diagram describing the potentiometers on the AMPLIFIER BOARD This diagram describes the current board 8 pots If the old board 14 pots is present it should be discarded and a new board ordered On the board test points extend out to the rear for convenience e VR612 613 See Signal Path Overview on page 21 and Electrical Calibrations on page 93 for details e The remaining six potentiometers should be adjusted together The detector should be equilibrated at 40 C for at least 90 minutes All DC measurements are with respect to chassis GND Amplifier Board Potentiometers VR614 VR613 Pos TP605 Polarity Amp Span P Polarity ox to Stag YR605 Control Output Neg Int t Polarity gt _ __ Me eae Amp TP604 Path Main ran stag VR607 Recorder S e VR612 knee Span VR61D Polarity to a Control Output ge Polarity Amp TP608 VR608 Service Handbook for 1047 Refractive Index Detector 12 2001 57 Hardware Information When the detector is equilibrated adjust the potentiometers in the order and by the procedures which follow 1 NJ OD OF Pp Ww Set the front panel FINE ZERO dial to it s mid position five turns Set the front panel INTEGRATOR dial fully clockwise Set the Response Time switch back panal to its mid position 0 8 s On the ELECTRICAL AUTOBALANCE BOARD set both poles of SW40
16. ERC75011A ERC751 ERC751 ERC751 04A 05A 06A 01040 22301 0100 0900 01047 04110 01047 60009 2110 0002 01047 66508 01047 66503 01047 66504 01047 66501 01047 66505 01047 66506 01047 66507 01047 24103 see Table 17 on page 138 0515 0761 01047 43701 0515 0094 3050 0225 01047 04101 01047 44101 01047 03701 01047 23201 0515 0915 2190 0586 132 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 15 Parts Information ERC number Part number Major Assemblies Description 38 Screw pan pozi M4 8 mm long Lock washer Flat washer 39 Screw pan pozi M3 6 mm long Lock washer Flat washer 40 Screw pan pozi M4 10 mm long Lock washer Flat washer 41 Screw pan pozi M4 12 mm long Lock washer 42 Nut hex M6 Flat washer 43 Screw hex M4 4 mm long 2 mm key 44 Standoff 7 mm dia x 7 mm height Extender board for items 22 23 24 25 26 and 27 not shown in figure 0515 0910 2190 0586 3050 0893 0515 0924 2190 0108 3050 0891 0515 0926 2190 0586 3050 0893 0515 0909 2190 0586 0535 0038 3050 0225 0515 0529 no p n 01047 66516 Service Handbook for 1047 Refractive Index Detector 12 2001 133 Parts Information Major Assemblies Figure 41 Service Handbook for 1047 Refractive Index Detector 12 2001 134 Table 16 Parts Information Front Panel Assembly The following table identifies the individual parts of the
17. Service Handbook for 1047 Refractive Index Detector 12 2001 Contents Hardware Troubleshooting 111 Detector Turn On Problems 111 Light Intensity Problems 111 Lamp Turn Problems 113 Limit Alarm AB Motor Drive and Balance Problems 113 Flush Valve Problems 116 Temperature Control Problems 119 Flow Cell Problems 121 Loss of Sensitivity 121 Chromatographic Troubleshooting Guide 122 General Characteristics of RI Detector 122 Optimizing your 1047A 123 Solvent Delivery System 123 Inlet Tubes 123 Outlet Tubes 123 Solvents 123 Troubleshooting the Chromatogram 125 Troubleshooting an Instable Baseline 126 6 Parts Information This chapter provides parts information for the 1047 Refrac tive Index Detector 127 Major Components 129 Major Assemblies 131 Front Panel Assembly 135 Rear Panel Assembly 138 Optical Unit 140 Cables 143 General 143 Signal Cables for Hewlett Packard Data Handling Devices 143 Unique 1047A Cables 143 Service Handbook for 1047 Refractive Index Detector 12 2001 Contents Inlet Outlet Tubes 145 8 Service Handbook for 1047 Refractive Index Detector 12 2001 General Information This chapter provides general information for the 1047 Refractive Index Detector General Information Safety Summary The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific war
18. attenuation then calculate the signal required for 1 mm deflection at that attenuation with this formula _ SignalFS Signal NTFS where signal signal for 1 mm deflection signal FS signal for full scale deflection INT FS integrator full scale deflection in mm Service Handbook for 1047 Refractive Index Detector 12 2001 101 Example of RIU mm Scaling Factor Calculation Table 6 Performance Verification The 1047A integrator output is calibrated such that 32 mV 1 x 10 5 RIU Calculate the RIU mm SCALING FACTOR for the integrator at the specific integrator attenuation Assume that the integrator needs a signal input of 32 mV for a full scale deflection at A TTENUA TION 5 This means a 1047A signal of 1 x 10 gt RIU will cause a FULL SCALE DEFLECTION of the integrator at ATTENUATION 5 Assume that the integrator has a full scale deflection of 150 mm 1x 10 RIU 8 oam 6 6x10 RIU Signal At attenuation 5 the scaling factor for this integrator is 1 x 10 gt RIU FS or 6 6 x 108 RIU mm RIU Scaling Factors for HP Integrators For the table below the full scale deflection on the HP 3390 2 3 is a 75 mm and on the HP 3394 6 is 150 mm RIU Scaling Factors for HP Integrators ATTN 339xA RIU FS 3390 2 3 RIU mm 3394 6 RIU mm 3 1 00x10 13x100 65x10 4 197x108 26x10 1 3x 10710 3 391x108 52x107 2 6 x107 2 781x10 10x10 5 2x 101 1 1 56x107 28x109 14x10 0 3 13x107 42x10 21x109 1 6 25 x107
19. for 1 64 x 10 RIU increment of detector response Most recorders have a variable FULL SCALE DEFLECTION setting 1 mV 10 mV and 1 V are most popular Ideally the RECORDER FULL SCALE DEFLECTION setting should be matched to the detector recorder output For example if the 10 mV RECORDER OUTPUT of the1047A is connected to a recorder the recorder s FULL SCALE DEFLECTION should be set to 10 mV In this wa y the detector response needed for a FULL SCALE DEFLECTION of the recorder is determined by the detector RANGE setting Peak height can be converted to detector response by it s percentage of FULL SCALE DEFLECTION Example The 1047A 1 mV RECORDER OUTPUT is connected to the recorder The recorder FULL SCALE DEFLECTION is set for 1 mV The 1047A RANGE dial is set to 16 x 10 5 RIU The chromatogram contains one peak which is 50 FULL SCALE DEFLECTION This peak then represents a detector response of 8x 10 RIU If it is not possible to properly match the detector recorder output and the recorder full scale deflection setting then the error will have to be taken into account during quantization or specifications verification Service Handbook for 1047 Refractive Index Detector 12 2001 105 Performance Verification Calculating Recorder The formula for calculating allowable deflection in mm at a specific detector Deflection RANGE setting is Recdefl SPES x RecFS Range where Recdef recorder deflection in mm Spec
20. minutes prevents any outgassing caused by mixing but is not usually long enough to change the solvent composition e The effectiveness of continuously degassing the mobile phase during the analysis depends on the solvents used and the length of the analysis Continuous degassing will prevent a degassed mobile phase from re absorbing air but there are two side effects to consider 1 Long term retention time changes with complex mobile phases If the mobile phase comprises a mixture of solvents and one of these solvents is more volatile than the others then continuous degassing can cause changes in retention times related to changing elution strength 2 Long term drift If the solvent trapped in the reference compartment may begin to degas at a fixed rate This will cause a continuously increasing refractive index difference between the solvent in the reference compartment and the continuously degassed flowing solvent in the sample compartment This effect will cause long term drift and is very noticeable when using for example THF The overall performance of the 1047A will vary depending on the solvent used Solvent properties such as viscosity and volatility are important factors to baseline stability 124 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information Troubleshooting the Chromatogram 1 Rule number one Turn off the flow and determine what portion of the problem noise drift wander e
21. motor can be driven to it s true center mid way position by simultaneously pressing the COURSE and COURSE buttons on the front panel The LIMIT alarm indicator flashes during this movement This centering of the motor does NOT cause a balance to occur If the relationship between the AB motor and the optical unit balance glass control shaft is correct this centering of the AB motor will result in a good optical null That is TP1 and TP2 will be similar in value A BALANCE command will then correctly establish the baseline Service Handbook for 1047 Refractive Index Detector 12 2001 113 Troubleshooting Information Table 10 Limit alarm AB motor and balance problems Possible Problem Actions Limit alarm is on but the AB motor does not respond to the centering command OR The AB motor does not respond to movement commands generally TP1 TP2 values do not respond to the movement of the AB motor Note This symptom often occurs if the detector is transported without tightening the locking screws The AB motor can be correctly centered from the LIMIT ALARM state but the LIMIT ALARM error occurs after a BALANCE is done BALANCE command initiated from the rear panel remote input does not work BALANCE command initiated from the front panel does not work 1 Check that all cables are connected correctly and tight check that all boards are seated securely 2 Replace the AB MOTOR assembly 3 Try the fo
22. the REC display and use the FINE ZERO dial to make the REC display read 0 00 4 Select the INT display Adjust VR701 so that the INT display reads 0 00 The FINE ZERO dial should now be able to simultaneously zero the REC and INT displays The INTEGRATOR dial should add 10 mV to the existing INT display Service Handbook for 1047 Refractive Index Detector 12 2001 97 Procedures Verify that the detector has adequate RANGE 1 For proper operation the detector must have adequate range in both the and directions Refer to AB Motor Assembly Replacement and Adjustment on page 73 The procedure for checking the relationship between the AB Motor and the optical balance glass actually does make sure that the detector has adequate range in both directions NOTE If the detector does not have adequate range in both directions the most likely cause is incorrect adjustment of the mirror particularly incorrect use of the mirror adjustment glass Refer to Mirror Adjustment on page 85 98 Service Handbook for 1047 Refractive Index Detector 12 2001 Performance Verification This chapter provides information on performance verification for the 1047 Refractive Index Detector Performance Verification This section is meant to assist in determining the actual performance of the 1047A Specification Verification e For the integrator output LJ calculating integrator deflection M verifying the noise
23. turns BALANCE the detector then use the FINE ZERO dial to make the INT display read 0 00 Press and hold the COURSE ZERO button The AB motor should be moving in the counter clockwise direction and the display reading must be increasing in the positive direction Make sure the sleeve coupling is firmly holding both the AB motor shaft and the optical balance glass control shaft Watch the INT display The display must reach 2 volts and go blank BEFORE the LIMIT ALARM indicator comes on While the detector is in the LIMIT ALARM state center the AB motor step C above When the motor has reached it s center position BALANCE the detector The INT display should return to the value established in step E 2 above Service Handbook for 1047 Refractive Index Detector 12 2001 75 If this test is NOT successful NOTE Procedures 9 Press and hold the COURSE ZERO button The AB motor should be moving in the clockwise direction and the display reading must be increasing in the negative direction Again watch the INT display The display must reach 2 volts and go blank BEFORE the LIMIT ALARM indicator comes on Finally again center the AB motor and balance the detector Repeat the AB motor adjustment procedure or Has the optical unit mirror been adjusted recently Incorrect use of the mirror adjust glass may be a cause Refer to Mirror Adjustment on page 85 for the mirror adjustment procedure or
24. unit 2 Refer to Figure 44 on page 140 Remove the optical unit top cover item 13 the insulation item 9 and the optics cover item 12 3 To identify the mirror refer to Figure 32 on page 81 preceding Do not touch the pitch screws 4 Remove the two mirror holding screws BE CAREFUL NOT TO TOUCH THE SURF ACE OF THE MIRROR Carefully remove the mirror and put it in a safe place If the detector is turned on now the LIGHT INTENSITY ERROR will come on 5 Carefully install the replacement mirror and insert the two mirror holding screws Do not tighten the mirror holding screws at this time instead proceed with the mirror adjustment procedure described in the next steps Mirror Adjustment This is a tedious adjustment that requires patience The mirror must be correctly adjusted to ensure proper operation of the detector 1 Activate the flush valve and flush the flow cell with pure isopropanol at a flow rate of between 4 and 5 ml min This is to make sure that there are no air bubbles in the flow cell Remember since the mirror is now mis adjusted display TP1 2 are not functional Make sure that there is no restriction downstream of the detector The flow cell can be destroyed by excessive back pressure Service Handbook for 1047 Refractive Index Detector 12 2001 85 Procedures 2 Fill both halves of the flow cell with pure well degassed HPLC grade water Set the detector temperature to 40 C and allow the detector t
25. width 0 04 Specifications of 1047A RID Signals Noise Wander Drift Response Time Flow Cell Recommended pH range Temperature Control Display Analog Outputs Communications Environment RI range 1 to 1 75 measurement range 1 64 x 10 DELTA RIU to 32 x 10 DELTA RIU full scale lt 2 5x 109 DELTA RIU peak to peak flowing water at 1 ml min 0 8 second response time 10 90 lt 1 x10 DELTA RIU lt 2 5x 107 DELTA RIU hour after warm up 0 3 0 8 or 5 seconds 10 90 7 ul volume with 7 bar 103 psi maximum pressure 2 3 to 9 5 standard Solvents with a pH between 1 0 and 2 3 must not contain acids which attack stainless steel 30 35 40 45 50 C Single line 4 1 2 digit display with real time display of output voltage recorder or integrator One output integrator output 2 V maximum 32 mV 10 DELTA RIU two recorder outputs 0 1 mV and 0 10 mV Four control inputs marker flush balance not ready Temperature range 0 to 40 C Service Handbook for 1047 Refractive Index Detector 12 2001 15 General Information Table 1 Specifications of 1047A RID Humidity lt 85 non condensing Power Requirements Line voltage 100 120 220 240 V AC 5 10 Line frequency 48 66 Hz Power consumption 120 VA 410 BTU hour Dimensions Height 150 mm 5 9 inch Width 430 mm 17 0 inch Depth 370 mm 14 6 inch Weight 19 kg 41 6 Ib 16 Serv
26. z O REF 9 No electrical calibrations are needed However the polarity of the Preamplifier board output should be checked as follows Turn on the detector turn on should be normal Then BALANCE the detector 66 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Procedures Select INT at the display The voltage should represent a normal balance based on the position of the front panel FINE ZERO and INTEGRA TOR dials Press and hold the COURSE ZERO button The display voltage must increase in the positive direction If the voltage increases in the negative direction re check the position of the white and green wires on the Preamplifier board Output Board DO NOT TOUCH VR701 Refer to Figure 43 on page 138 and proceed as follows 1 Disconnect the large ribbon cable item 1 from the OUTPUT board the other end of this cable goes to Motherboard CON114 Disconnect the 4 wire ribbon cable from the OUTPUT board this cable is part of the Response Time Switch assembly item 4 Working from the outboard side of the rear panel remove the board holding clamps item 3 These clamps are located on each side of both the INPUTS and OUTPUTS terminal blocks The OUTPUT board may now be removed To remove the board It will be necessary to bend the rear panel item 6 slightly outward You may also decide that it is more convenient to remove the six boards from the card cage I
27. 1 to ON Using VR604 adjust TP604 visible to measure 0 VDC 1 mV Using VR605 adjust TP605 visible to measure 0 VDC 1 mV Using VR607 adjust TP607 under VR605 to measure 0 VDC 1 mV Using VR608 adjust TP608 under VR607 to measure 0 VDC 1 mV On the ELECTRICAL AUTOBALANCE BOARD set both poles of SW401 to OFF Adjust VR615 as follows Set the front panel RANGE dial to 32 and make sure the front panel recorder POLARITY button is OFF set to positive recorder polarity Press BALANCE Measure TP607 under VR605 Use the front panel COURSE ZERO button to make TP607 measure 2 5 VDC Now measure TP608 under VR607 Using VR615 adjust TP608 to measure 2 5 VDC Test this calibration by using the front panel recorder POLARITY button and observing the front panel REC display The REC value should equal the REC value If needed use VR615 to fine tune the REC value Adjust VR614 as follows Make sure the front panel integrator POLARITY leds indicates positive integrator polarity integrator polarity is switchable only at the rear panel The front panel FINE ZERO and INTEGRATOR dials should be set as described in step 1 above At the front panel select the INT display Press BALANCE Use the FINE ZERO and INTEGRATOR dials to make the INT display read 0 00 and press BALANCE again 58 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Measure TP604 visible Use th
28. 1047A Refractive Index Detector Service Handbook o FEC BEOORDE REOOROER REFERENCE MTO REORDER om CHAE aano L a a om mogo FINE ZERO TEMPERAURE C RANGE x10 RIU F S i HP 1047A OC C Q Teorann RI DETECTOR O UMIT ALARM LIGHT THROUGHPUT ERROR oe Agilent Technologies Copyright Agilent Technologies 2001 All rights reserved Reproduction adaption or translation without prior written permission is prohibited except as allowed under the copyright laws Part No NONE 12 2001 Printed in Germany Warranty The information contained in this document is subject to change without notice Agilent Technologies makes no warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material IMPORTANT NOTE This version of the 1047 service manual includes all sections from the original manual 01047 90100 edition 1 May 1991 Part numbers have been updated as of 12 2001 Some part numbers are marked bold with These may not be available Contact your local Agilent support off
29. 7 85401 15 Screw pan pozi M3 12mm long 0515 0911 Lock washer 2190 0108 16 Screw pan pozi M3 6 mm long 0515 0924 Lock washer 2190 0108 17 Screw pan pozi M3 16 mm long 0515 0920 Lock washer 2190 0108 18 Screw flat pozi M3 8 mm long 0515 0907 19 Screw pan pozi M4 8 mm long 0515 0910 Lock washer 2190 0586 Flat washer 3050 0893 20 Nut 2950 0072 Washer int tooth 2190 0679 21 Nut 0535 0025 Flat washer 3050 0002 22 Screw pan pozi M3 6 mm long 0515 0924 Lock washer 2190 0108 23 Nut M3 0535 0004 Heat Sink Assembly 01047 60005 consisting of items 11 13 and 14 Service Handbook for 1047 Refractive Index Detector 12 2001 139 Parts Information Optical Unit The following table identifies the individual parts of the Optical Unit as shown in Figure 44 Figure 44 Optical Unit 140 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 18 Parts Information Optical Unit Description Part number N A Complete Optical Unit see page 129 1 Lamp Holding Collar 01047 22501 2 Lamp Assembly 01037 60002 3 Lamp Support Plate Assembly 01047 04104 4 Lens Slit Assembly 01047 60014 5 Mirror Assembly 01047 60015 6 Cell Assembly 01037 60003 7 Photo diode Lens Assembly 01047 60013 8 Insulation 20 mm thick in 2 feet by 2 feet pieces 01047 45403 9 Insulation 15 mm thick in 2 feet by 2 feet pieces 01047 45402 10 Insulation 10 mm thick in 2 feet by 2 feet pieces 01047 45402 11 O
30. 8 3 x109 42x10 2 1 25 x10 17x108 8 5 x10 3 250x106 3 3x10 17x108 4 5 00 x10 6 7 x10 2 34x108 102 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 6 Performance Verification RIU Scaling Factors for HP Integrators ATTN 339xA RIU FS 3390 2 3 RIU mm 3394 6 RIU mm 5 1 00 x10 13x107 6 5 x 10 2 6 2 00x 10 26x107 14x107 7 4 00 x10 53x107 27x107 8 8 00 x10 13x10 53x107 3 1 60x10 21x108 10x10 10 3 20x10 43x10 22x10 Verifying the Noise Specification Noise Specification 2 5 x 10 RIU P P equivalent to 5 x 10 RIUpp 2 For HP 3390 2 3 at ATTENUA TION 2 5x10 RIUpp Noise Se Ob ee eI ee 1x10 RIU mm Allowable Noise at attenuation 2 5 mm peak to peak pp for HP 3390 2 3 3 For HP 3394 6 at ATTENUATION 2 5x10 RIUpp Noises aee 0 5x 10 RIU mm Allowable Noise at attenuation 2 10 mm P P for HP 3394 6 Service Handbook for 1047 Refractive Index Detector 12 2001 103 Performance Verification Verifying the Drift Specification Drift Specification 2 5 x 10 7 RIU hr equal to 250 x 10 RIU hr 1 For HP 3390 2 3 at ATTENUATION 1 250 x 10 RIU Drift 2 8 3 x 10 RIU mm Allowable drift at attenuation 1 30 mm hr for HP 3390 2 3 2 For HP 3394 6 at ATTENUATION 1 250 x 10 RIU Drift lt L 4 2 x 10 RIU mm Allowable drift at attenuation 1 60 mm hr for HP
31. 92 Optical Unit Heater Temperature Sensor 92 Electrical Calibrations 93 NULL the detector output 93 Calibrate the LIGHT INTENSITY ERROR THRESHOLD 94 Calibrate the Integrator Output Span 95 Calibrate the Recorder Output Span 95 Calibrate the Autobalance Decision Threshold 96 Verify the Operation of the Baseline Controls and Autobalance 96 Service Handbook for 1047 Refractive Index Detector 12 2001 5 Contents Calibration of OUTPUT board VR701 97 Verify that the detector has adequate RANGE 98 4 Performance Verification This chapter provides information on performance verifica tion for the 1047 Refractive Index Detector 99 1047A Specifications Verification 101 For the Integrator Output 101 Calculating Allowable Integrator Deflection 101 Example of RIU mm Scaling Factor Calculation 102 RIU Scaling Factors for HP Integrators 102 Verifying the Noise Specification 103 Verifying the Drift Specification 104 Verifying the Wander Specification 104 For the Recorder Outputs 105 The Range Dial and Recorder Outputs 105 The Recorder Full Scale Deflection Setting 105 Calculating Recorder Deflection 106 Verifying the Noise Specification 106 Verifying the Drift Specification 106 Verifying the Wander Specification 107 For the ChemStation 107 Example Calculating the noise 107 Example Calculating the Drift 107 5 Troubleshooting Information This chapter provides troubleshooting information for the 1047 Refractive Index Detector 109 6
32. Board 56 Output Board 56 Amplifier Board 57 Digital Display Switch Board 60 Digital Display Board 60 3 Procedures This chapter provides procedures for the 1047 Refractive Index Detector 61 General Information Read this first 62 Board and Front Panel Controls Replacement 64 Temperature and Motor Control Board 64 Power Supply Board 64 Optical Auto Balance Board 64 Auto Balance Board 65 Control Board 65 Amplifier Board 65 Preamplifier Board 65 Output Board 67 Motherboard 68 4 Service Handbook for 1047 Refractive Index Detector 12 2001 Contents Boards and Controls located in the front panel 68 TEMPERATURE SELECT SWITCH assembly RANGE SWITCH BOARD assembly FINE ZERO ADJUST assembly or INTEGRATOR ADJUST assembly 70 LED BOARD 1 KEY SWITCH BOARD LED BOARD 2 DIGITAL DISPLAY BOARD or DISPLAY SWITCH BOARD 70 Flush Valve Replacement 71 AB Motor Assembly Replacement and Adjustment 73 Replacement 73 Adjustment 75 To check for the correct relationship between the AB motor and the optical balance glass proceed as follows 75 If this test is NOT successful 76 Power Supply Component Replacement 77 Optical Unit Replacement 78 Inside the Optical Unit 80 Lamp Replacement Intensity Adjustment 80 Flow Cell Replacement 83 Mirror Replacement 85 Mirror Adjustment 85 The basic task 88 Helpful hints 88 If it seems not possible to adjust the mirror 89 Photo Diode Replacement Adjustment 90 Replacement 90 Adjustments and Calibrations
33. Calibrations on page 93 Service Handbook for 1047 Refractive Index Detector 12 2001 59 Hardware Information Digital Display Switch Board On this board the INT display and REC display offsets are calibrated Proceed as follows 1 Set the front panel FINE ZERO dial to it s mid position 5 turns Set the front panel INTEGRATOR dial fully clockwise Both INTEGRATOR and RECORDER polarity should be set to positive With the detector on disconnect one of the lamp wires from the VALVE LAMP terminal block This will cause a LIGHT INTENSITY ERROR At the front panel select the INT display and press BALANCE Using VR1302 closest potentiometer to the optical unit adjust the INT display to read 0 VDC 1 mV At the front panel select the REC display and press BALANCE Using VR1301 closest potentiometer to the display adjust the REC display to read 0 VDC 0 02 mV Re connect the lamp wire The LIGHT INTENSITY ERROR should clear and the baseline controls should function normally Digital Display Board On this board the display span is calibrated Proceed as follows 1 Set the front panel FINE ZERO dial to it s mid position 5 turns Set the front panel INTEGRATOR dial fully clockwise Both INTEGRATOR and RECORDER polarity should be set to positive At the front panel select the INT display and BALANCE Observe the DISPLAY SWITCH BOARD The circuit side of the switch board is viewed from the top There is only
34. Control Problems When the circuit is working normally the front panel TEMPERATURE indicator led is e Solidly ON when both rear panel switches are first turned on e FLASHING when the actual temperature as measured by the Optical Unit temp sensor equals the temperature setpoint e OFF if the actual temperature exceeds the temperature setpoint Moderately sensitive work can be done after 90 minutes of temperature equilibration However sensitive work or specification verification requires at least 24 hours of temperature equilibration Temperature Control Problems Possible Problem Possible Causes and Actions The Temperature Control circuit seems to be functioning 1 The ambient environment is too erratic for good normally but the no flow detector baseline is temperature control Consult page 3 7 of the 1047A unacceptable Operator s Handbook Make sure the detector environment is acceptable 2 The detector temperature setting is not high enough Make sure that the temperature setting is at least 10 C above ambient temperature 3 The TEMP AND MOTOR CONTROL board is defective Replace the board 4 The Optical Unit heater or temperature sensor is defective Replace the Optical Unit The front panel TEMPERATURE indicator led does not turn 1 Check the following connections for tightness on when the rear panel TEMP switch is turned on a CON203 on the TEMP AND MOTOR CONTROL board This is the temp sensor connec
35. ER SUPPLY board OPTICAL AUTOBALANCE board ELECTRICAL AUTOBALANCE board CONTROL board AMPLIFIER board To DISPLAY SELECT SWITCH board CON2 To OUTPUT board CON703 To front panel INTEGRATOR dial ro mMm mMm Oo Dd wo YS v O FTA C 38 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Table 3 Cables termination Term Description Q To front panel TEMPERATURE select switch R To RANGE SWITCH board CON1001 S To LED BOARD 2 CON903 T To rear panel TEMP switch Figure 13 Motherboard Layout A A A bo CON CON con con CON CON b CON 01 102 119 103 104 105 CON 118 108 57 1 ON 1 o M B1 M BI Al B1 Al B1 Al B1 Al g1 1 G 6 D M CON 10 G H J K L R AA 12 1 CON 115 5 E CON ar 109 ON 1 06 1 N ws F e i NFT 5 SD A 1 i 1 A A A Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information AB Motor Control Figure 14 shows the relationship of the Optical Balance Motor to the Optical Unit The motor shaft and the optical balance glass control shaft are joined together by a sleeve coupling The motor can be commanded to go to it s mechanical center position Ideally the mechanical center position of the of the motor should cause the optical balance glass t
36. Front Panel Assembly as shown in Figure 42 on page 137 Front Panel Assembly Front Panel Assembly Description ERC number Part number 1 Rotary Knob 01047 47401 2 Set Screw M3 hex 3 mm long 2 mm key 0515 0154 3 Front Panel Cosmetic Plug 01047 47601 4 Front Panel only 01047 00201 5 Solenoid flush Valve 01047 60008 Fasteners for solenoid valve Same as item 22 6 Temperature Select Switch Assembly 01047 60001 7 LED Board 1 01047 66510 8 Push Button Cap 01047 47403 9 Key Switch Board ERC75006B 01047 66509 10 Ribbon Cable 1 from Key Switch Board 01047 61603 CON601 to Motherboard CON115 11 Ribbon Cable 3 from Range Switch Board 01047 61605 CON1001 to Motherboard CON116 12 Range Switch board Assembly 01047 66512 13 Fine Zero Adjust Assembly 01047 60003 14 Integrator Adjust Assembly 01047 60004 15 Digital Display Board ERC75012 01047 66513 16 Display Push Button Cap 01047 47402 Service Handbook for 1047 Refractive Index Detector 12 2001 135 Parts Information Table 16 Front Panel Assembly Description ERC number Part number 17 LED Board 2 ERC75009B 01047 66511 18 Display Switch Board ERC75013 01047 66514 19 Ribbon Cable 2 from LED Board 2 CON901 01047 61604 to Motherboard CON109 20 Ribbon Cable 5 from Display Switch Board 01047 61607 CON1302 to Motherboard CON111 21 Nut 2950 0043 22 Screw pan pozi M4 8 mm long 0515 0910 Lock washer 2190 0586 Flat washer 3050 0893 23 Screw pan pozi
37. H or the TEMP SENSOR will immediately disable power to CON201 and turn off the temperature indicator LED In addition these TEMPERATURE AND MOTOR CONTROL board test points are useful TP201 upper right corner e Pulsing approx 2 5 VDC while temperature is controlling at the setpoint e 14 VDC while actual temp is less than setpoint e 14 VDC while actual temp is greater than setpoint or TEMP SELECT SWITCH is disconnected TP203 under CON201 e HIGH 5 VDC during any normal operation e LOW if TEMP SENSOR is disconnected Service Handbook for 1047 Refractive Index Detector 12 2001 45 Hardware Information Flush Valve Control Electronics Figure 17 outlines both the manual and remote operation of the FLUSH VALVE 12 VDC is applied to the LAMP VALVE terminal valve wires for valve turn on 12 VDC is removed for valve turn off The front panel FLUSH button must be off if the rear panel remote input is to be used The remote input needs a contact closure short for activation The first contact turns on the valve the next contact closure turns off the valve The front panel FLUSH button will be disabled if the remote input is held in the constantly short state CONTROL BOARD TP506 LOW for valve off HIGH for valve on POWER SUPPLY BOARD TP305 to TP306 12 VDC The front panel FLUSH light comes on when the valve is turned on either from the front panel or the rear panel The light only indicates that the valv
38. IFF AMP is first taken to the AB autobalance DECISION threshold circuit Measuring TP3 VR503 is adjusted such that the AB DECISION threshold is 16 x 10 RIU The output of the AB DECISION threshold circuit is then taken from the CONTROL board to the input of the OPTICAL AUTOBALANCE board Figure 5 The DIFF AMP output is also taken from the CONTROL board to the input of the ELECTRICAL AUTOBALANCE board s Figure 6 on page 26 ELECTRICAL AB CIRCUITS Optical Balance Board from Control Board AB Decision Level to Electrica Output AB Board wo Con 119 Ultimately to the AB Motor Assembly Optical AB N Motor Drive Sensing Pulse Circuit and Control Circuit Optical Autobalance Board When a BALANCE is initiated the OPTICAL AUTOBALANCE board judges the signal from the CONTROL board AB DECISION threshold circuit If the signal is below the AB threshold then only an electrical balance is done Service Handbook for 1047 Refractive Index Detector 12 2001 25 Figure 6 NOTE Hardware Information Electrical Autobalance Board from Control to Output Board Board Diff Response Time Output Controller S Con 103 VR401 TP401 Sw401 Electrical AB Circuit Electrical AB aye Controller TP40 Electrical Autobalance Board During an electrical autobalance the ELECTRICAL AUTOBALANCE BOARD sets the output of it s ELEC
39. NOTE Replacement Procedures e Replace the optical unit covers and insulation e Perform the complete program of electrical calibrations described in Electrical Calibrations on page 93 Photo Diode Replacement Adjustment The Photo Diode Assembly 01047 60013 actually consists of the photoed package and the 1st lens slit group Never attempt to further disassemble the photo diode assembly The relationship of the internal parts are critical and these are pre adjusted at the factory 1 Refer to Parts Information on page 127 2 Remove the detector top cover Refer to Figure 44 on page 140 Remove the optical unit top cover item 13 the insulation item 9 and the optics cover item 12 Locate the Preamplifier board Refer to Figure 40 on page 130 if needed 4 Carefully un solder from the Preamplifier board the three wires white green clear from the photo diode assembly cable This cable can be seen coming from the optical unit directly under the lamp Before un soldering note the position of the three wires 5 Refer to Boards and Controls located in the front panel on page 68 and Figure 27 on page 68 This figure shows the holding screws for the detector front panel assembly Remove these screws then carefully allow the front panel assembly to fall away it is not necessary to remove any cables or tubes but make sure that nothing is damaged 6 Figure 38 on page 91 shows a side view of the opti
40. Refer to Optical Unit Replacement on page 78 92 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Electrical Calibrations Correct electrical calibration is essential to the proper operation of the 1047A These calibrations must be performed if The original factory set light intensity is altered e g by changing the lamp or re adjusting the mirror A critical board which is replaced e g the AMPLIFIER or CONTROL board The calibrations MUST BE PERFORMED IN THE ORDER IN WHICH THEY ARE GIVEN BELOW Pure well degassed HPLC grade water should be in both halves of the flow cell during calibrations The detector should be equilibrated at 40 C for ninety minutes Average light in tensity TP1 2 select buttons pressed together MUST be no less than 3 5 NULL the detector output 1 Turn on the detector and make sure that the LIGHT INTENSITY ERROR is not present At the front panel turn the INTEGRATOR dial fully clockwise and turn the FINE ZERO dial to it s mid position 5 turns Press and hold the front panel COURSE ZERO button until the LIMIT ALARM indicator comes on With the LIMIT ALARM on set the optical balance glass to it s null position by pressing the and COURSE ZERO buttons simultaneously The LIMIT ALARM indicator will flash as the optical balance glass moves to it s null position Press the BALANCE button After the balance is done select the INT display U
41. SPAN POLARITY CONTROL and INT OFFSET stages INT OUTPUT SPAN is adjusted by VR613 POLARITY can only be changed from the detector rear panel INT OFFSET is influenced by the front panel INTEGRATOR dial and by VR701 on the OUTPUT board Figure 9 on page 30 The output of the INT OFFSET stage is taken to both the digital display and the INT analog output at the rear panel The INT analog output is calibrated OUTPUT SPAN such that 32 mV equals 1 x 10 RIU Service Handbook for 1047 Refractive Index Detector 12 2001 27 Hardware Information Figure 7 Amplifier Board from Output Board Response to Output Board Time Controller Int Offset Path Int Polarity Control Activated from Rear Panel only Integrator Path pan M a D arker Range z Ra Offset E Control n Circuit ad Marker A o Also controllable from Rear Panel Front Panel 28 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Figure 8 Amplifier Board to Output Board to Output Board ImV Output to Display 10mV Output MB Can 114 MB Con 111 MB Con 114 103 ae Von Amplifier Board 1mV Rec Shorting Polarity Control FS Output Select Ornv Control Rec Circuits Control Polarity Recorder Service Handbook for 1047 Refractive Index Detector 12 2001 29 Hard
42. TRICAL AB CIRCUITS to zero An electrical balance does not zero the DIFF AMP output If the AB decision signal exceeds threshold an optical balance is also done This means that the OPTICAL AUTOBALANCE board indirectly moves the Optical Balance Glass until the output of the DIFF AMP is zero The optical balance is then followed by an electrical balance Refer to the AB MOTOR CONTROL for details of the optical unit and autobalance motor control On the ELECTRICAL AUTOBALANCE board both poles of SW401 must be OFF If these switches are pushed ON the ELECTRICAL AB CIRCUITS output will be held constantly to zero The ON position of these switches is for adjusting the offset of the ELECTRICAL AB CIRCUIT output VR401 is adjusted so that TP401 is 0 VDC 0 3 mV When SW401 is OFF TP402 HIGH normally LOW during balance TP403 HIGH normally LOW when balance command initiated 26 Service Handbook for 1047 Refractive Index Detector 12 2001 Integrator Path Hardware Information The detector signal path continues at the output of the ELECTRICAL AUTOBALANCE board The signal goes through the RESPONSE TIME circuit and is then taken to the input of the AMPLIFIER board Figure 7 on page 28 and Figure 8 on page 29 The first stage of the AMPLIFIER board is influenced by the front panel FINE ZERO dial After the first stage the signal divides into two separate paths INTEGRATOR AND RECORDER The INTEGRA TOR path consists of the INT OUTPUT
43. and samples used in Liquid Chromatography are often TOXIC and DANGEROUS Always determine if the detector is filled with any harmful liquid before beginning a repair e ALWAYS USE ANTI STATIC PRECAUTIONS when working on the 1047A e Certain adjustmen t and calibration procedures require that the detector cover be removed while the detector is powered on BE VERY CAREFUL WHILE PERFORMING THESE PROCEDURES Hazardous voltages are present This warning will be assumed for all written procedures Service Handbook for 1047 Refractive Index Detector 12 2001 63 CAUTION CAUTION NOTE Procedures Board and Front Panel Controls Replacement Refer to Figure 40 on page 130 for board locations Each board is keyed to it s particular location The key is inserted in to the particular Motherboard connector Be careful not to remove this key Do not touch any potentiometer or switch on any board that is not described in the Electrical Calibration procedures These potentiometers and switches are pre set at the vendor s factory and may not be touched without risk to the proper operation of the board Always disconnect power before removing replacing any board Temperature and Motor Control Board Remove the defective board and insert the replacement board Make sure that the three connections for the board CON201 202 and 203 are correctly attached Each connector is unique and cannot be put in to an incorrect p
44. ard Install the replacement board and re connect the associated cables No calibrations are needed Re assemble the detector and check for proper operation 70 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Flush Valve Replacement 1 Disconnect the flush valve wires from their locations at the VALVE LAMP terminal block Remove the two screws that secure the valve and remove it 2 Install the replacement flush valve 3 Connect the valve wires to their locations at the VALVE LAMP terminal block There is no polarity either wire can be attached to either location WARNING If the flush valve is incorrectly plumbed the detector flow cell may be destroyed Make sure that the flush valve is plumbed according to the diagram in Figure 29 Figure 29 Flush Valve Plumbing Detector Outlet FA 3 Way Fitting Detector N Inlet ji a D Optical Unit View Flush Valve from Front FAN B i gt O O a 3 Z Service Handbook for 1047 Refractive Index Detector 12 2001 71 Procedures Table 5 Legend Flush Valve Plumbing Term Description See the 1047A Operator s Handbook for details of Flow System Operation Read these notes first A This is a standard zero dead volume 1 16 inch union
45. b holder as shown in Figure 34 on page 83 Repeat this procedure until the replacement lamp can be correctly adjusted in the detector 82 Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 34 NOTE Procedures Orientation of Bulb Holder V p WJ G uf Possible original Changed orientation orientation Flow Cell Replacement The Flow Cell Assembly 01037 60003 may be used in either the 1047A or the obsolete 1037A The four capillary tubes of the flow cell are not fitted with screws ferrules at the time of shipment Included with the flow cell assembly is a bag which contains a set of fittings for the 1047A and a set of fittings for the 1037A Select the fittings required see Figure 29 on page 71 The optical surfaces of the flow cell itself are protected by a piece of clear plastic tape Remove this tape and clean the optical surfaces with alcohol and a cotton swab Failure to remove this protective tape will result in insufficient light intensity The lamp should be correctly adjusted at this time If not refer to Lamp Replacement Intensity Adjustment on page 80 directly preceding 1 Refer to Parts Information on page 127 2 Remove the detector top cover If needed refer to Figure 40 on page 130 to identify the optical unit 3 Refer to Figure 44 on page 140 Remove the optical unit top cover item 13 insulation item 9 and optics cover item 12 4 Ref
46. cal unit as viewed from the front of the detector The figure also shows how the photo diode assembly will be removed from the optical unit 90 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Figure 38 Optical Block Photo diode Assembly Setscrew Access Holes 1 5 mm HEX 2 Mirror Adjust Glass Access Hole 3 Optical Unit Outer Case 4 Optics Block 5 Foam Insulation 6 Photo diode Assembly 7 Atthis time observe how the photo diode assembly is seated in the optical unit 8 The photo diode assembly is secured by two 1 5 mm hex set screws These screws can be loosened through the access holes with a 1 5 mm hex wrench Push a hex wrench through the foam insulation into the access holes in the optics block Loosen both set screws 9 The complete photo diode assembly can now be pulled out of the optics block as shown in Figure 38 10 Install the replacement photo diode assembly Make sure to guide the photo diode cable through the correct hole in the front plate the hole directly under the lamp Make sure the new photo diode assembly is adjusted correctly it should be pushed against the front plate and sitting flatly on the floor of the optics block Then tighten the set screws Service Handbook for 1047 Refractive Index Detector 12 2001 91 Adjustments and Calibrations Procedures 11 Solder the three wires of the photo diode cable to their correct positions on th
47. d ALARM does not turn on The motor sits vibrating at it s 2 Replace the AB MOTOR assembly mechanical stop In all other ways the AB motor and NOTE The vibrations may have disturbed the AB BALANCE functions are normal MOTOR OPTICAL BALANCE GLASS relationship Verify this relationship on page 75 step B Service Handbook for 1047 Refractive Index Detector 12 2001 115 Troubleshooting Information Flush Valve Problems NOTE Normally a quiet click can be heard when the valve activates The FLUSH indicator led on the front panel only indicates that a flush command has been made The indicator DOES NOT sense the actual position of the valve Therefore the indicator cannot be taken as proof that the valve is actually in one position or the other If the valve is activated from the front panel the rear panel remote input is disabled If the valve is activated from the rear panel remote input the front panel FLUSH button is disabled Table 11 Flush Valve Problems Possible Problem Actions Valve leaks liquid externally WARNING The pressure rating of the valve is only 2 bar Valve has an internal cross port leak This symptom will most likely be noticed in the form of a baseline drift while the valve is in the normal position The drift stops when the valve is activated Valve cannot be activated from the rear panel remote input Replace the valve This is most often caused by excessive r
48. detector power Turn on should be normal and the LIGHT N 3 INTENSITY ERROR should not be on By hanad adjust the optical balance glass control shaft so that TP1 and TP2 display are equal This means that the optical balance glass is in it s null position At the front panel press and hold the COURSE ZERO button until the AB motor reaches it s limit counter clockwise and the LIMIT ALARM indicator is on While the LIMIT ALARM indicator is on press the and COURSE ZERO buttons simultaneously The AB motor will now seek it s mechanical center position The LIMIT ALARM indicator will be flashing during this movement At this time the optical balance glass is in it s null position and the AB motor is in it s mechanical center position Now the balance glass and the AB motor may be joined together As carefully as possible push the AB motor assembly onto the optical unit allowing the sleeve coupling to join the optical balance glass control shaft to the AB motor shaft Make sure that the sleeve coupling has a firm hold on both sides Fasten the AB motor to the optical unit with the original four screws This question is often asked After step D above how close must TP1 and TP2 be to each other In other words how may the relationship between the AB motor and the optical balance glass be checked Select INT at the display Turn the front panel INTEGRATOR dial fully clockwise Center the front panel FINE ZERO dial 5
49. dicator LED turns on TP1102 normally HIGH LOW for LIMIT ALARM From the LIMIT ALARM state the AB Motor can be commanded to move to it s mechanical center position by pressing the front panel and COURSE ZERO buttons simultaneously The motor will then move in the appropriate direction until it s center position is sensed A BALANCE is NOT automatically done after this movement 42 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Hardware Information Temperature Control Electronics Figure 16 on page 44 is a functional block diagram of the 1047A heater control electronics Actual heating power is provided by the main power transformer 100 V AC secondary which can be measured at CON202 of the TEMP AND MOTOR CONTROL board A TEMPERATURE REGULATOR delivers power to the optical unit HEATER The optical unit TEMP SENSOR reports the actual optical unit temperature to the TEMPERATURE CONTROL circuit Required heater voltage is determined by a comparison between the actual optical unit temperature and the temperature setpoint Both poles of switch SW201 must be OFF for correct operation of the heater control circuitry The ON position of these poles is used by the factory and will disable operation of the heater circuit The HEATER is protected by a thermal fuse which opens at about 76 C The heater circuit is protected by a 2 A fuse Resistance of the HEATER about 190 Q cold Resistance of the TEMP
50. e A D Converter The Noise Specification is 5 x 10 9 RIU P P 5x10 9 0 0005 x 10 5 The allow able Noise output is 16 pV P P See 1 above The Drift Specification is 2 5 x 10 7 RIU hr 2 5 x 107 0 025 x 10 5 The allowable Drift output is 800 pV hr Service Handbook for 1047 Refractive Index Detector 12 2001 107 Performance Verification 108 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information This chapter provides troubleshooting information for the 1047 Refractive Index Detector Troubleshooting Information The following sub sections are guides for troubleshooting general categories of hardware problems These guides are meant to be used in conjunction with the detailed descriptions found in Hardware Information on page 19 e Hardware Troubleshooting 1 Detector turn on problems I Light intensity problems 1 Lamp turn on problems 1 Limit alarm AB motor and balance problems J Flush valve problems 1 Temperature control problems d Flow cell problems LI Loss of sensitivity e Chromatographic Troubleshooting 110 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information Hardware Troubleshooting Detector Turn On Problems Table 7 Detector turn on problems Problem Check Rear line switch turned on no response or incorrect Is the line switch functional detector turn on Is the line power se
51. e has been commanded to turn on it DOES NOT guarantee that the valve itself is functioning correctly 46 Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 17 Hardware Information Flush Valve Control Electronics Output Board LED Board 1 O Flush LED Input Row gt a Can 602 Key Switch Board Flush Regulator Set 12VD0 5VDC con 104 Valve Driver Circuits e TPSO06 Control Board Lamp Valve Terminal Flush Vane Service Handbook for 1047 Refractive Index Detector 12 2001 47 Figure 18 Hardware Information Remote Control Function Electronics The detector rear panel is arranged into an INPUT row and an OUTPUT row The Outputs Light Intensity Error and Balance Not Ready For both of these functions the appropriate pin pair is normally open The Light Intensity Error pair is closed as long as the error exists and opens again when the error clears The Balance Not Ready pair is closed for the duration of the BALANCE Block Diagram Light Intensity Error Output Board pg Output Row gt 4 l Light Intensity Error n CON 703 kid Front Panel Light Intensity Error LED other CON 109 CON 114 Board Control Board Ligh
52. e Preamplifier board If you did not note their original positions refer to Figure 26 on page 66 12 Replace the optical unit top covers and insulation Replace the detector front panel assembly How much work needs to be done depends up on how closely the new photo diode assembly matches the original photo diode assembly Proceed as follows 1 Turn on the detector The INTENSITY ERROR should not be on The average intensity display TP1 2 pressed together MUST be no less than 3 5 However remember that typical intensity values range from 4 0 to 5 5 TP 1 2 must also be within 0 2 of each other 2 In your best judgement If step 1 above is satisfactory immediately perform the complete calibration program described in Electrical Calibrations on page 93 If step 1 above is NOT satisfactory then a Is there an air bubble in the flow cell Try flushing the flow cell or b Is the new photo diode assembly correctly installed Is it adjusted correctly Are the photo diode wires correctly soldered to the Preamplifier board or c The new photo diode assembly requires a re adjustment of the mirror Refer to Mirror Replacement and Mirror Adjustment on page 85 and re adjust the mirror Optical Unit Heater Temperature Sensor The optical unit heater or it s thermal fuse and temperature sensor ARE NOT replaceable on the component level If these components become defective the complete optical unit must be replaced
53. e front panel COURSE ZERO button to make TP604 read 2 5 VDC This is near the maximum possible output for TP604 The front panel INT display will usually blank out before 2 5 VDC is reached However TP604 must reach 2 5 VDC before the front panel LIMIT ALARM is set Now measure TP605 visible Using VR614 adjust TP605 to measure 2 5 VDC Press BALANCE the INT display should return to 0 00 To test this calibration Locate the manual shaft turning knob on the OPTICAL BALANCE MOTOR If needed see Figure 31 on page 74 item C Carefully turn the manual shaft turning knob exactly two full turns in the counter clockwise direction The value of the INT display should be increasing in the positive direction Note the value of the INT display at the two turns position At the rear panel change the integrator POLARITY to negative by shorting the two POLARITY pins in the INPUT row This will initiate a balance and the INT display should return to 0 00 The front panel integrator POLARITY leds should now indicate negative integrator polarity Again carefully turn the manual shaft turning knob exactly two full turns in the counter clockwise direction The value of the INT display should now be increasing in the negative direction In value of the INT display should now be the same as the value in step 3 above but with a negative polarity 10 Perform the complete program of electrical calibrations described in Electrical
54. ecial care when handling the detector 12 Service Handbook for 1047 Refractive Index Detector 12 2001 General Information About this Manual This handbook provides service information for the 1047A Refractive Index Detector The handbook has been divided into the informational sections outlined in the table of contents Service Handbook for 1047 Refractive Index Detector 12 2001 13 General Information Repair Policy All printed circuit boards are replaced on the board level Certain other electronic components may be replaced on the component level The Optical Unit may be replaced as an assembly or repaired on the component level depending on the failure mode Certain replacements require subsequent mechanical and electrical adjustments These adjustments are documented in this handbook The components and assemblies available for replacement parts are described in Parts Information on page 127 Warranty Agilent Technologies warranty policies and procedures apply 14 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 1 General Information Specifications 1047A specifications for noise drift and wander are made under the following conditions Flow of 1 ml min of adequately degassed HPLC grade water 24 hour warm up time at detector temperature of 40 C and ambient temperature lt 25 C Detector response time 0 8 seconds For Hewlett Packard integrators peak
55. en 100 and 110 mV this is below the AB decision threshold 5 Press BALANCE Only an electrical autobalance should be done the AB Motor should not move The INT display should return to 0 00 as originally set 6 Again press and hold the COURSE ZERO button this time until the INT display reads between 150 and 160 mV this is above the AB decision threshold 96 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Procedures 7 Again press BALANCE This time the AB Motor should move to it s null position as an optical autobalance is done Then an electrical autobalance should return the INT display to 0 00 as originally set It is important to realize that a BALANCE will return the baseline to the value set by the FINE ZERO and INTEGRA TOR dials A BALANCE does not return the baseline to 0 00 unless as in the test above the actual set value is 0 00 Calibration of OUTPUT board VR701 Optional step Ordinarily VR701 of the OUTPUT board need not be calibrated Perform this procedure only if the relationship between the INT display and the REC display is not correct refer to Verify the Operation of the Baseline Controls and Autobalance on page 96 step 2 is not correct 1 The OUTPUT BOARD is located on the detector rear panel assembly VR701 is at the top of the board clearly labeled NULL the detector as described in Null the detector output Set the front panel RANGE switch to 32 Select
56. er outputs polarity light above the front panel recorder POLARITY button A change in polarity will also cause a BALANCE to occur Block Diagram Integrator Polarity Output Board Input Row gt f e Polarity CON _703 Front Panel Integrator Polarity LED O O CON 114 CON 109 Mother Board Optical Auto nedor Balance e Polarity Board A a EAA Control Circuits Amplifier Board Auto Balance Board Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information MARKER The MARKER applies a voltage spike to the recorder outputs so that the start of a chromatogram may be noted on a strip chart recorder A MARKER can be initiated from the front panel or by applying a short to the MARKER pin pair Figure 22 Marker Board Output Marker o e Input Row gt CON 703 Front Panel Marker LED Panel Button Front Marker CON 114 CON 115 Mother Board Recorder Qutput Circuits Relay and Driver Marker Voltage Offset Amplifier Board Board Control 52 Service Handbook for 1047 Refractive Index Detecto
57. er to Figure 32 on page 81 to identify the flow cell assembly as it appears in the uncovered optical unit Also note the location of the mirror Service Handbook for 1047 Refractive Index Detector 12 2001 83 NOTE NOTE NOTE Procedures Do not disturb the mirror pitch screws 5 Remove the t wo mirror holding screws BE CAREFUL NOT TO TOUCH THE SURF ACE OF THE MIRROR Carefully remove the mirror and put it in a safe place If the detector is turned on now the LIGHT INTENSITY ERROR will come on 6 The two screws which hold the flow cell in place are hidden under the mirror With the mirror removed these screws can now be seen Remove the two flow cell holding screws but do not attempt to remove the flow cell assembly now 7 The four capillary tubes from the flow cell assembly can be seen coming from the front of the optical unit One of the tubes goes to the detector inlet Two of the tubes go to the flush valve The last tube goes to the 3 way fitting Now disconnect these four capillary tubes 8 Refer again to Figure 44 on page 140 Carefully extract the complete flow cell assembly item 6 as indicated in the figure This may be tedious As the flow cell assembly is pulled upward two thin pieces of insulation covering the capillary tubes will be displaced 9 Apply asmall amount of thermal compound part number 6040 0454 to the replacement flow cell assembly Usually the residual thermal compound fr
58. es an overview of DC power production and distribution All test points are clearly labeled across the top of the POWER SUPPLY board The REGULATOR SET is mounted on the rear panel These regulators contribute to the production of the 5 VDC LAMP supply and the 12 VDC supply The REGULATOR SET cable is connected to MOTHERBOARD CON107 To check the performance of the REGULATOR SET DC measurements can be made directly at the wires of CON107 5 VDC lamp TP 302 to BLU com almost zero GND TP 302 to WHITE in 8 3 VDC TP 302 to RED out 5 VDC 12 VDC TP 306 to ORN com almost zero GND TP 306 to YEL in 19 VDC TP 306 to BRN out 12 VDC Lamp voltage is supplied to the LAMP as soon as the rear panel LINE switch is turned on Circuits on the CONTROL board apply 12 VDC to the FLUSH VALVE when a FLUSH command is initiated 34 Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 11 Mother Board x from Main XFMR secondaries Power Von AC Voltages Hardware Information DC Power Distribution Regulator Set 5VDC a Lamp mounted on Rear Panel 2p er 6B a 12v0C ab fal Jg ov slia
59. estriction downstream of the valve Check for downstream restrictions inside the detector Check for excessive restriction downstream of the detector itself Note f the 1090 LC is being used the detector outlet SHOULD NOT be plumbed to the 1090 injector wash input Replace the valve Can the valve be activated and deactivated normally from the front panel If yes 1 The front panel FLUSH button must be off to use the rear panel remote input 2 Make sure that a contact closure is being applied to the remote control input normally open closed for activate deactivate 3 Check the OUTPUT board ribbon cable In this case other remote control functions will likely also be defective 116 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information Table 11 Flush Valve Problems Possible Problem Actions Valve cannot be activated from the front panel FLUSH button 4 Replace the OUTPUT board If no Is the FLUSH command applying 12 VDC as needed to the valve drive wires If yes replace the valve If no 1 Replace the CONTROL board 2 Replace the POWER SUPPLY board Can the valve be activated and deactivated normally from the rear panel remote input If yes 1 Deactivate the valve from the rear panel then check for correct front panel operation 2 Check the KEY SWITCH board ribbon cable In this case other front panel functions are likely to also be defective
60. evel detector repair Temperature and Motor Control Board e SW201 Both poles must be OFF see Heater Control Electronics on page 44 for details e TP201 upper right corner see Heater Control Electronics on page 44 for details e TP203 under CON201 see Heater Control Electronics on page 44 for details e TP204 205 left of CON201 see AB Motor Control on page 40 for details Power Supply Board All test points are clearly labeled across the top of the board See Power Distribution on page 32 for details Optical Autobalance Board VR2 TP1101 1102 See AB Motor Control on page 40 for details Service Handbook for 1047 Refractive Index Detector 12 2001 55 Hardware Information Electrical Autobalance Board e SW401 Both poles must be OFF see Signal Path Overview on page 21 for details e TP401 402 403 see Signal Path Overview on page 21 for details Control Board Test points extend out to the rear for convenience e VR501 TP1 4 See Signal Path Overview on page 21 for details e VR502 503 TP2 3 See Signal Path Overview on page 21 and Electrical Calibrations on page 93 for details Output Board e VR701 See Signal Path Overview on page 21 and Electrical Calibrations on page 93 for details 56 Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 24 Zero Input Hardware Information
61. ex Detector 12 2001 21 Hardware Information Figure 2 Signal Path Overview Display Switch Board Digital Display LED Board 2 Light Intensity MB Con 109 Error Preamplifier Board gt R eZ MB Con 104 MB Con 119 Intensity Error Signal Path AB Level Starts here Sensing and Control AB Decision Level Diff AB Motor Control Control Board Optical Autobalance Board 22 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Figure 3 Signal Path Overview A Ink Adjust Dial Time J gwiteh 10 mVI Con 704 1mV Electrical AB Circuit Response Time Controller 10mV INT Electrical AB Control Output Board Elec Autobalance Board 1mV ME Con 116 MB Con 105 1001 Range Switch Board Con Integrator Circuits First 1mV Stag Recor 1002 Con 1OmV Circuits Amplifier Board lA Zer Front Panel P Recorder Controls Service Handbook for 1047 Refractive Index Detector 12 2001 23 Figure 4 Hardware Information On the Preamplifier board S and R each produce a voltage output proportional to the light reaching it s photo diode These voltages are re
62. g screws while pressing the mirror Mirror adjustment can be done with the optical unit completely uncovered but stray light will cause some instability of TP1 2 After the mirror is finally tightened cover the optical unit Then the mirror adjust glass can be used to make TP1 2 equal 88 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures If it seems not e Is there an air bubble in the flow cell possibts i e Has the flow cell been replaced recently If yes has the clear protective mirror tape been removed from the flow cell e Did you get fingerprints or dirt on the optical balance or mirror adjust glass e Is the mirror a new replacement part If yes it could be that an additional step is needed for mirror adjustment See Figure 37 Figure 37 Mirror Screws 1 Mirror Holding Screws 2 Mirror Pitch Screws 2 mm HEX Ordinarily the pitch screws should not be loosened However if the mirror is a new part the pitch of the mirror may also need adjustment Proceed as follows a Loosen the mirror holding screws Establish the mirror position at midway between full left and full right Then tighten the holding screws b Loosen the pitch screws Adjust the mirror pitch so that the beam is related to the photo diodes as shown in Figure 36 on page 88 Then re tighten the pitch screws c Repeat the mirror adjustment procedure Service Handbook for 1047 Refractive Index Detector 12 2001 89
63. ht intensity Correct the intensity loss then perform the complete program of electrical calibrations described in Electrical Calibrations on page 93 If the OUTPUT SPANS are correct there is a small possibility that the flow cell is defective However it is most likely that the loss of sensitivity is related to the chromatographic method the Liquid Chromatograph or a worsening detector environment Service Handbook for 1047 Refractive Index Detector 12 2001 121 Troubleshooting Information Chromatographic Troubleshooting Guide General Characteristics of RI Detector Because they respond to all solutes RI detectors have excellent versatility Generally speaking success depends up on how carefully certain mobile phase and environmental requirements are observed For maximum sensitivity the mobile phase and the sample solute should have different an RI as possible However even under optimum conditions the RI detector has only modest sensitivity when compared to the UV detector The RI detector is generally never used for trace analysis A limitation to the real sensitivity of RI detectors is their susceptibility to the environment temperature changes being the biggest problem To even begin to achieve the theoretical sensitivity of the detector the ambient environment and the chromatographic system parameters temperature flow etc must be kept under close control These factors most often result in baseline pr
64. ical Unit temp sensor may have resistance but be defective Replace the Optical Unit 120 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information Flow Cell Problems Flow cell problems can present the following symptoms Table 13 Flow Cell Problems Possible Problem Actions External leaks Replace the flow cell Refer to Flow Cell Replacement on page 83 This is usually caused by excessive restriction downstream of the cell Liquid may be seen coming from the contingency leak drain on the right side of the detector The leak may also cause light intensity problems and baseline problems such as noise drift and wander Note The back pressure rating of the flow cell is only 7 bar Internal cross compartment leaks or u cracks which Same as above aspirate In this case an external leak may not be observed This defect often causes light intensity problems and baseline problems such as noise drift and wander Loss of Sensitivity This complaint is usually expressed as an observed diminishment in peak height under the identical chromatographic conditions which previously produced satisfactory results When this complaint is made IMMEDIATELY verify that the INTEGRATOR and RECORDER OUTPUT SPANS are correctly calibrated A low OUTPUT SPAN will result in diminished peak height If the OUTPUT SPANS are low determine what has caused the loss in detector lig
65. ice Handbook for 1047 Refractive Index Detector 12 2001 Figure 1 General Information Pressure Contribution of Detector Typically the pressure contribution of the detector NOT SPECIFIED is no greater than 35 bar at 5 ml min IPA The difference between VALVE ON and VALVE OFF mode is no greater than 1 bar Pressure Contribution A P A Px Figure 1 shows how the pressure is measured Pressure Diagram HP 1090 5ml min mus HP 1047A IPA APx APY Service Handbook for 1047 Refractive Index Detector 12 2001 17 General Information 18 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information This chapter provides hardware information for the 1047 Refractive Index Detector Hardware Information Detection principle optical system and detector flow path Refer to the 1047A Operator s Handbook 01047 90000 The section OVERVIEW of the Operator s Handbook contains detailed information on the detection principle optical system and flow path of the 1047A 20 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Signal Path Overview Figure 2 on page 22 and Figure 3 on page 23 show the functional signal path block diagram for the 1047A More detailed block diagrams and fundamental circuit explanations will be found in the following text Service Handbook for 1047 Refractive Ind
66. ice in case of part number issues or upgrades The latest version of this manual is available as Adobe Acrobat Reader PDF version only and can be downloaded from the Agilent Technolgies web page www agilent com Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Contents 1 General Information This chapter provides general information for the 1047 Refrac tive Index Detector 9 Safety Summary 10 General 10 Operation before applying power 10 Safety Symbols 11 1047A related warnings 12 About this Manual 13 Repair Policy 14 Warranty 14 Specifications 15 Pressure Contribution of Detector 17 2 Hardware Information This chapter provides hardware information for the 1047 Re Sractive Index Detector 19 Detection principle optical system and detector flow path 20 Signal Path Overview 21 Integrator Path 27 Recorder Path 30 Power Distribution 32 AC Power Distribution 32 DC Power Distribution 34 Power Supply Area Wiring Diagram 36 Motherboard 38 AB Motor Control 40 Temperature Control Electronics 43 Service Handbook for 1047 Refractive Index Detector 12 2001 3 Contents Flush Valve Control Electronics 46 Remote Control Function Electronics 48 The Outputs 48 The Inputs 50 Recorder Control Electronics 53 Summary of Switches and Potentiometers 55 Temperature and Motor Control Board 55 Power Supply Board 55 Optical Autobalance Board 55 Electrical Autobalance Board 56 Control
67. if contacted result in personal injury 10 Service Handbook for 1047 Refractive Index Detector 12 2001 WARNING General Information Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible and when inevitable should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or perform any unauthorized modification to the instrument Capacitors inside the instrument ma y still be charged even if the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Safety Symbols Below you will find some symbols that are used on the instrument and throughout the documentation The apparatus will be mark ed with this symbol when it is necessary for the user to refer to the instruction manual in order to protect the apparatus against damage Indicates dangerous voltages Indicates a protected earth terminal
68. ifier board ribbon cable from CON501 of the CONTROL board 2 Refer to Figure 41 on page 134 item 33 pre amp support plate Remove the two screws which hold the support plate to the card cage Then pull the complete assembly up for access Be CAREFUL the Preamplifier board is still attached to the Optical Unit by the photo diode assembly cable Service Handbook for 1047 Refractive Index Detector 12 2001 65 Figure 26 Procedures Remove the Plexiglas safety cover from the Preamplifier board The three wires of the photo diode assembly cable white clear and green are soldered to the Preamplifier board Note the location of these wires on the board At the Preamplifier board carefully un solder the three wires of the photo diode cable Remove the standoffs which fix the Preamplifier board to it s support plate then remove the board Attach the replacement Preamplifier board to the support plate Carefully solder the three wires of the photo diode cable to the new Preamplifier board If needed refer to Figure 26 Replace the Plexiglas safety cover then re attach the complete Preamplifier board assembly to the card cage Re connect the Preamplifier board ribbon cable to CON501 of the CONTROL board Preamplifier Board to Optical Unit to CON 501 Photo Diode Assembly Board 7 O SAMP las Re ane Pies Wl 7 VEAR BLK ns BRN lt Z RED 2 ORN C y c z YEL zZ Oo D GREEN Ss N C
69. ill usually be near zero Flow cell defective leaking internally between compartments or leaking externally Flush valve defective the user has attempted to purge air bubbles but half of the cell retains air because the valve is defective Flow cell dirty optical surfaces are coated with an opaque material In this case the TP1 2 intensities will be abnormally low but not zero Lamp does not turn on when the line switch is turned on In this case TP1 2 intensities will be zero Lamp not correctly adjusted In this case TP1 2 intensities will be abnormally low or zero depending on the degree of mis adjustment INTENSITY ERROR THRESHOLD CALIBRATION incorrect In this case the actual light intensity is sufficient but the intensity error circuit is incorrectly calibrated The TP1 2 intensities seem correct but the INTENSITY ERROR indicator is on If calibrations seem correct likely failures are PREAMPLIFIER board ribbon cable not securely connected to CONTROL board CON501 In this case TP1 2 readings will be 0 00 Activate the flush valve and flush with a flow rate higher than the analytical flow rate Many times it will be necessary to flush with pure methanol or even isopropanol to purge the air bubbles Replace the flow cell and perform the alignments and calibrations described in Procedures on page 61 See Flush Valve Problems 1 Perform an appropriate flushing procedure 2 Has the flow cell ju
70. increases The rate of heat transfer changes as the flow rate changes this can cause baseline instability until thermal equilibrium is reached The 1047A inlet tube should be insulated using the pipe insulation 0890 1032 this ensures that the thermal stability in the flow system is easier to achieve The1047A outlet tube should have a wide internal diameter to avoid high back pressures which can cause baseline instability or even damage the flow cell The tubing should not be coiled as this can cause pressure pulses which produce reproducible spikes on the baseline e All solvents must be HPLC grade e Solvents must not contain any particulate matter and must be thoroughly degassed before use Careful filtering removes suspended particulate matter which might contaminate the cell walls or reduce sensitivity by light scattering Degassing prevents bubbles forming in the solvent as it passes through the flow cell e Filters should not exceed 2 pm in pore size The filter material must not react with the solvent to cause solvent contamination Service Handbook for 1047 Refractive Index Detector 12 2001 123 Troubleshooting Information e Ifthe mobile phase is a combination of two or more solvents these solvents must be thoroughly mixed One method for solvent preparation is to first separately degas the pure solvents and then mix them to form the desired solvent composition followed by a further 10 minutes degassing This extra 10
71. lation item 9 and optics cover item 12 Removing these covers will expose the optics as shown in Figure 32 on page 81 3 Disconnect the existing lamp wires from their locations at the VALVE LAMP terminal block Loosen the lamp holding collar note that the collar is secured by set screws 1 5 mm hex located in the collar Remove the lamp holding collar and remove the lamp assembly 4 Fix the replacement lamp assembly into the lamp holding collar Insert the lamp into the optical unit and loosely screw the lamp in to place with the holding collar Do not tighten the collar at this time Connect the lamp wires to their positions at the VALVE LAMP terminal block There is no polarity either wire can be connected to either position 5 Turn on detector power Refer to Figure 32 on page 81 The light from the lamp should be clearly seen in the area of the slit Now rotate the lamp until the slit is completely covered by the light as shown in Figure 33 on page 82 If it seems not to be possible to correctly adjust the lamp see the special information note below step 8 80 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Figure 32 Inside the Optical Unit 3 4 2 3 1 Mirror Assembly Fi o DIP h 2 Mirror Pitch Screw 2 mm HEX E x J 3 Mirror Holding Screws 4 Cell Assembly 4 5 Slit Lens Assembly z 5 6 Slit
72. ll z ORN 2 Lamp gt ey Mother Valve Lamp Board Terminal K e e Con 107 ORN af A ORN S BRN s Distributed to general electronics 5 G BRN Con 102 ier i Valve TP301 5VDC Lamp N Rect R301 TP302 5VDC Lamp GND Con 105 ne R P305 12VDC Flush for all pC TP306 12VDC GND WW Valve Voltages e TP303 5VDC Main Circuit Value 579 P304 DN Control Board Ripple not e TP307 15VDC greater than amp P308 A CND 10mv p p TPS09 15VDC Power Supply Board may be measured here x Lamp Voltage measured at the Terminal 29 6 3 0 VDC Service Handbook for 1047 Refractive Index Detector 12 2001 35 Table 2 Hardware Information Power Supply Area Wiring Diagram Figure 12 shows details the wiring of the power supply area This figure should be used when a power supply component needs to be replaced Legend Power Supply Wiring Term Description 1 To TEMP AND MOTOR CONTROL board CON202 To MOTHERBOARD CON113 To MOTHERBOARD CON110 Orange to top bottom right posts of TEMP switch Gray to transformer White to top left post of TEMP switch Gray to bottom left post of TEMP switch Orange from top post of voltage selector to transformer oOo ama N oon A WwW N Black from middle post of voltage selector to bottom right post of LINE switch 10 Blue from bottom post of voltage selector to transformer a Sai White from top left post of LINE switch to line fu
73. llowing boards a TEMP AND MOTOR CONTROL board b OPTICAL AB board c AB board d POWER SUPPLY board e KEY SWITCH board Make sure that the motor is turning the optical unit balance glass control shaft Replace or adjust the coupling sleeve Then perform the alignment procedure for the AB motor assembly Make sure that the flow cell is free of air bubbles Make sure that the INTENSITY ERROR is not present Purge the cell of bubbles if needed If the detector intensity is normal replace the AB board and OPTICAL AB board Does the front panel BALANCE button work correctly If yes 1 Make sure that the remote signal being applied is a contact closure normally open closed for BALANCE 2 Check the OUTPUT board ribbon cable in this case other remote control functions are likely to also be defective 3 Replace the OUTPUT board If no Replace the AB board and the OPTICAL AB board Does the rear panel BALANCE remote input work correctly 114 Service Handbook for 1047 Refractive Index Detector 12 2001 Troubleshooting Information Table 10 Limit alarm AB motor and balance problems Possible Problem Actions If yes 1 Check the KEY SWITCH board ribbon cable In this case other front panel functions are likely to also be defective 2 Replace the KEY SWITCH board If no Replace the AB board and the OPTICAL AB board The AB motor reaches it s mechanical limit but the LIMIT 1 Replace the OPTICAL AB boar
74. ment on page 83 Check for corroded contacts in the temperature selector switch refer to Table 15 on page 131 Clean or replace the selector switch Check for poor quality solder connections of the Photo Diode wires at the Pre Amplifier board Carefully re solder the connections 126 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information This chapter provides parts information for the 1047 Refractive Index Detector Parts Information This section provides diagrams for parts identification and the complete parts listings respectively e Major Components e Major Assemblies e Front Panel Assembly e Rear Panel Assembly e Optical Unit e Cables e Inlet Outlet Tubes e Screws NOTE Part numbers have been updated as of 12 2001 Some part numbers are marked bold with These may not be available Contact your local Agilent support office in case of part number issues or upgrades 128 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 14 Parts Information Major Components The following table identifies the major components of the 1047A with top cover removed as shown in Figure 40 on page 130 Major Components Description ERC number Part number N A Optical Unit see Table 15 on page 131 1 LED Board 1 ERC75008A 01047 66510 2 Key Switch Board ERC75006B 01047 66509 3 Display Select Switch Board ERC75013 01047 66514 4 Digital Displa
75. nings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies former Hewlett Packard Company assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation before applying power comply with the installation section Additionally the following shall be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it should be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay etc are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Adjustments described in the manual are performed with power supplied to the instrument while protective covers are removed Energy available at many points may
76. nstall the replacement OUTPUT board Re attach the holding clamps and re connect both ribbon cables Turn on the detector turn on should be normal Verify that each function of the OUTPUT board works correctly No electrical calibrations are necessary However refer to Verify the Operation of the Baseline Controls and Autobalance on page 96 step 2 for information on when OUTPUT board VR701 may need adjustment Service Handbook for 1047 Refractive Index Detector 12 2001 67 NOTE Figure 27 Procedures Motherboard 1 2 3 4 Remove the six boards from the detector card cage Disconnect all cables from the motherboard Remove the six screws which secure the motherboard and remove it Install the replacement motherboard and secure it with it s six screws Re connect all cables refer to Motherboard Layout on page 39 if necessary Replace the six card cage boards then check for proper turn on and operation No calibrations are needed Boards and Controls located in the front panel 1 2 Remove the detector top cover Refer to Major Assemblies on page 134 Remove the side panels items 3 and 15 by removing screws item 4 Disconnect the tubes connected to the IN OUT block item 17 Re connecting these tubes incorrectly will destroy the flow cell Refer to Figure 29 on page 71 for correct plumbing Figure 27 represents a top view of the detector with the detector top cover
77. nt Panel Assembly 01047 60002 2 Bottom Casting 01047 24102 3 Top Cover 01047 24103 4 Screw M5 9 3 mm long 0515 0750 5 Main Power Transformer 01047 61101 6 Optical Unit with AB motor 01047 60017 without AB Motor 01047 60018 7 Shock Absorbing Foot 4040 2089 8 Three Way Fitting 01047 27601 9 Capillary Tube Assembly 01047 60010 10 Capillary Tube Assembly 01047 60011 11 Preamplifier Board ERC75100H 01047 66502 12 Optical Balance Motor Assembly includes 01047 60016 complete Assembly plus holding bracket 12a Cable 01047 61608 13 Support Stand Assembly 01047 60012 14 Leak Pan 01047 43201 15 Side cover left 01047 04103 16 Bulkhead Nut 01046 25701 Service Handbook for 1047 Refractive Index Detector 12 2001 131 Table 15 Parts Information Major Assemblies Description ERC number Part number 17 Capillary Holder 18 1 16 ZDV union INLET 19 Metal Floor 20 Lamp Valve Terminal Assembly 22a Fuse 2A 21 Motherboard 22 Temperature and AB motor Control Board 23 Power Supply Board 24 Optical Auto Balance Board 25 Auto Balance Board 26 Control Board 27 Amplifier Board 28 Top Cover 29 Rear Panel Assembly 30 Hex Set Screw 31 PC Guide Rail 32 Screw hex M6 30 mm long 5 mm key Flat Washer 33 Preamp Support Plate 34 Preamp Plastic Cover 35 Shaft Connection Sleeve 36 Wide bore 1 16 union outlet 37 Screw pan pozi M4 6 mm long Lock washer ERC75201A ERC751 ERC751 02A 03A
78. o equilibrate at 40 C for ninety minutes 90 minutes yet Refer to Figure 32 on page 81 preceding Set both the optical balance glass and the mirror adjust glass to be perpendicular to the light beam best estimation by eye is ok Note that the mirror adjust glass movement is accessed through a hole in the side of the optical unit This hole may be reached by removing the front panel cosmetic plug Figure 42 on page 137 item 3 For this purpose use aa long flat blade screwdriver with a blade no more than 4 mm wide Figure 35 shows the location of the photo diodes inside the optical unit The photo diodes can be seen by looking from the rear of the optical unit as shown The Figure also shows the possible movements of the mirror during adjustment 86 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures Figure 35 Location of Photo Diodes Glasses Front View Top View Photodiode Mirror Holding Screw He JA Pitch R Fis j G Halding Screw wy 5 Turn on the detector and look at the photo diode area By hand move the mirror such that the light beam falls equally on the photo diodes as shown in Figure 36 Before you start read the general information below for an overview of the job and some helpful hints Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 36 The basic task Helpful hints Procedures Light beam on Pho
79. o be perpendicular to the beam as shown in the figure The optical balance glass is then in it s null position Figure 14 Relationship Optical Balance Motor Optical Unit manual shaft optical balance optical mirror turning knob glass control balance adjustment shaft glass glass tical 01777 c z optica l l Pa beam ol O shaft balance L presne ie egm Se motor ena cig sleeve coupling i Optical Unit Partial Top View Figure 15 shows the electronic control circuits of the optical balance motor Legend for Figure 15 A Commands for motor drive circuit B Motor drive pulses C Actual motor position signal 40 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Figure 15 Control Circuits of Optical Balance Motor AB Motor Assy Temperature and Mator Control Board C C 9 Er cs TP205 oter Drive Mtr Pos Sensor 5 Circui oO TP204 NAVV TTR 12 r 12 j oc Con 101 p Na Rey Con 119 Con 109 Motor Drive Motor Sense 5 Con 903 A ane ww Pulse Ckts a VRZ e TF1101 Q Limit Alarm LED Board 2 Limit Ala
80. oblems which interfere with accurate quantization and even with automation Another severe limitation of RI detectors is that they are impractical to use in gradient analysis It is extremely difficult to adequately match the RI of sample and reference streams during gradient formation Optimizing your 1047A on page 123 describes certain key points to observe when optimizing the 1047A system for best performance 122 Service Handbook for 1047 Refractive Index Detector 12 2001 Solvent Delivery System Inlet Tubes Outlet Tubes Solvents Troubleshooting Information Optimizing your 1047A In this section we describe some practical hints which can help you optimize the use of your 1047A e The solvent delivery system of your liquid chromatograph must produce a very stable and pulse free flow Pressure pulses in the flow stream cause baseline noise and drift e Ifthe solvent delivery system of your liquid chromatograph has a multichannel capability the mobile phase should be pre mixed in a reservoir and pumped from one channel only e With the 1090 HPLC we recommend that the unused solvent delivery channel lines are flushed with the same solvent for your analysis This eliminates the possibility of baseline instability caused by small amounts of different solvent leaking into the mobile phase stream e The flow rate can affect baseline stability Noise caused by eddy currents in the flow cell increases as flow rate
81. og output at the rear panel The 10 mV recorder output is taken to both the digital display and the rear panel The voltage RIU relationship for the recorder outputs is determined by the RANGE SWITCH setting Important points in the signal path block diagram e When a BALANCE is done the ELECTRICAL AUTOBALANCE board does set the output of ELECTRICAL AB CIRCUITS to zero However the actual signal outputs of the detector will be whatever value has been set by the position of the front panel FINE ZERO and INTEGRATOR dials e The front panel FINE ZERO dial influences both RECORDER and INTEGRATOR outputs The front panel INTEGRATOR dial influences only the INTEGRATOR output e At the display selecting functions TP1 and TP2 displays the actual signal present at PREAMPLIFIER board TP1 and TP2 respectively e At the display selecting functions TP1 and TP2 simultaneously displays the average of TP1 and TP2 Service Handbook for 1047 Refractive Index Detector 12 2001 31 Hardware Information Power Distribution AC Power Distribution Figure 10 shows details the AC power distribution of the 1047A AC secondary voltages may be measured at motherboard CON110 as shown The 100 V AC secondary for the heater may be measured at CON202 of the TEMP and MOTOR CONTROL board The rear panel TEMP switch must be on for this measurement NOTE The present AC power supply only employs two primary windings 115 V AC orn and 240 V AC blu as
82. om the original flow cell assembly is enough to use on the new assembly 10 Carefully press the new flow cell assembly into place in the optical unit Take care to position the flow cell tubes correctly Take care to replace the thin pieces of insulation which originally covered the capillary tubes Secure the flow cell in place with the two flow cell holding screws Incorrect plumbing will destroy the flow cell 11 Re plumb the flow cell assembly capillary tubes as shown in Figure 29 on page 71 Correct routing of the stainless steel flow cell capillaries is important for good baseline stability Poorly routed capillaries can put strain on the flow cell causing excessive baseline drift and wander 12 Carefully replace the mirror Screw the two mirror holding screws in to 84 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE NOTE Procedures place but do not tighten them down at this time 13 Perform the Mirror Adjustment on page 85 procedure Mirror Replacement The lamp should be correctly adjusted at this time If it is not refer to Lamp Replacement Intensity Adjustment on page 80 If the mirror has not yet been removed begin with step 1 below If the mirror has already been removed and is now loosely back in place begin immediately with Mirror Adjustment on page 85 1 Remove the detector top cover If needed refer to Figure 40 on page 130 to identify the optical
83. one test pin on the switch board labeled TP1 the label may not be present on very old detectors TP1 is used for the DIGITAL DISPLAY BOARD span calibration Measure TP1 with respect to chassis GND At the front panel use the COURSE ZERO button to make TP1 read 180 mV VR1201 is the only potentiometer on the DIGITAL DISPLAY BOARD By adjusting VR1201 make the INT display read 1800 mV In other words the INT display should read 10 times the value of TP1 with the opposite polarity 60 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures This chapter provides procedures for the 1047 Refractive Index Detector Procedures General Information Read this first The figures in Parts Information on page 127 may be used as needed as instructions for the remov al of certain 1047A sub assemblies and parts Several of these procedures refer to this chapter The top cover of the 1047A is removed as shown in Figure 25 The top cover must be removed for most of the procedures described herin Figure 25 Top Cover Top Top Screw Screw OD p ee Screw DcCrew Front 62 Service Handbook for 1047 Refractive Index Detector 12 2001 Procedures e ALWAYS DISCONNECT POWER before removing any board or disconnecting any cable in the 1047A This basic advice will be assumed for all written procedures e Remember that solv ents
84. or shaft should still be adjusted to two full turns counter clockwise with the INT and REC OUTPUT SPANS correctly calibrated 2 Locate VR503 and TP3 on the CONTROL board Set up the DC Voltmeter to measure TP3 with respect to chassis GND 3 Adjust VR503 so that TP3 reads 0 5 VDC The detector Autobalance circuit is now adjusted so that the Electrical Optical Autobalance decision threshold is 4 x 10 5 RIU Verify the Operation of the Baseline Controls and Autobalance 1 The AB motor shaft should still be adjusted to two full turns counter clockwise The front panel INTEGRA TOR dial should be set fully clockwise and the FINE ZERO dial should be at it s mid position 5 turns The RANGE knob should be set to 32 2 Press BALANCE After the balance both the REC and the INT displays should be theoretically 0 00 The FINE ZERO dial which affects both INT and REC should provide similar baseline range in both the and direction The INTEGRATOR dial when turned fully counter clockwise should add 10 mV to the existing value of the INT display The REC display is not affected by the INTEGRATOR dial If the relationship between the INT display and REC display is not correct refer to the procedure Calibration of OUTPUT board VR701 on page 97 3 Select the INT display and use the baseline control dials to set the INT display to a baseline value of 0 00 4 Press and hold the COURSE ZERO button until the INT display reads betwe
85. osition No electrical calibrations are needed Both poles of SW201 should be OFF Power Supply Board Remove the defective board and insert the replacement board No electrical calibrations are needed Optical Auto Balance Board DO NOT TOUCH VR2 Remove the defective board and insert the replacement board No electrical calibrations are needed 64 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE NOTE NOTE NOTE Procedures Auto Balance Board DO NOT TOUCH VR401 Remove the defective board and insert the replacement board No electrical calibrations are needed Both poles of SW401 should be OFF Control Board DO NOT TOUCH VR501 Remove the defective board and insert the replacement board Make sure to connect the ribbon cable from the pre amplifier board to CON501 correctly Perform the complete program of electrical calibrations described in Electrical Calibrations on page 93 Amplifier Board DO NOT TOUCH VR615 608 607 614 605 or 604 Remove the defective board and insert the replacement board Perform the complete program of electrical calibrations described in Electrical Calibrations on page 93 In older detectors the original AMPLIFIER board may have 14 potentiometers The current AMPLIFIER board has only 8 potentiometers The current AMPLIFIER board is a direct replacement for the original board Preamplifier Board 1 Disconnect the Preampl
86. presentative of light intensity and are useful in detector troubleshooting The intensity voltages are first taken to the CONTROL board From the CONTROL board Figure 4 they are taken directly to the front panel DIGITAL DISPLAY where S or R can be selected as TP1 or TP2 respectively at the display Control Board to Electrical to Optical AB Board AB Board to Display to LED Board 2 MB Con 111 MB Con 109 Con 104 Control Board Int TP2 Error Buffer e Soca a AVG Preamplifier Board TF 1 e me D TP2 5 p Detector Signal R x Ly Path begins here VR501 VR503 AR TP3 3 Decision gt ___ Buffer Next the average intensity typically between 4 0 and 5 5 is taken to the CONTROL board INTENSITY ERROR threshhold circuit Measuring TP2 VR502 is adjusted such that the INTENSITY ERROR is set if the original factory intensity changes by approx 25 TP4 is normally HIGH and LOW for error 24 Service Handbook for 1047 Refractive Index Detector 12 2001 Figure 5 Hardware Information The detector signal path actually begins at the output of the DIFF AMP With the detector on and the lamp wires disconnected from the LAMP VALVE terminal the offset of the DIFF AMP should be 0 5 mV measured at TP1 adjusted with VR501 The output of the D
87. ptical Unit Housing 01047 25201 12 Optics Cover 01047 04105 13 Optical Unit Top Cover 01047 04106 14 Teflon Drain Tube 5021 7115 15 Leak Pan Spring 01047 29101 16 Lamp socket 01047 25202 17 Holding screw for top cover and optics housing total 12 M3 0515 0924 6 mm long Washer 3050 0891 18 Holding screw for optics cover lens slit assembly not shown 0515 0912 and cell assembly total 10 M3 8 mm long Washer 3050 0891 19 Mirror holding screw total 2 M4 15 mm long 0515 0909 Service Handbook for 1047 Refractive Index Detector 12 2001 141 Parts Information Table 18 Optical Unit Description Part number Small washer 2190 0586 Large Washer 3050 0894 20 Support plate holding screw total 4 M4 18 mm long 0515 1073 flat pozi 21 Mirror pitch set screw total 2 M4 hex 4 mm long 2 mm key 0515 0529 22 Set screw for photo diode assembly and lamp holding collar 0515 0154 total 3 M3 hex 3 mm long 1 5 mm key 142 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Cables General Refer to the 1047A Operator s Handbook 01047 90000 for specific information on the connection of cables At the 1047A all inputs and outputs are terminal connectors which accept spade type fittings or even bare wires Signal Cables for Hewlett Packard Data Handling Devices Table 19 Signal Cables 1047A to others Description Part number HP 3390 2
88. r 10 Remove the AB Motor assembly from the optical unit It will eventually be attached to the replacement optical unit see Figure 30 on page 73 78 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE NOTE Procedures 11 Carefully place the new optical unit into the detector At this time secure the optical unit by replacing the four nuts which secure the optical unit support feet The two locking screws should be screwed into their holes but not tightened 12 Carefully re solder to the preamplifier board the three wires of the optical unit photo diode assembly cable If you did not make a note of the correct position of the three wires refer to Figure 26 on page 66 Then replace the safety cover 13 Reconnect the optical unit leak drain tube to the leak drain fitting at the rear of the optical unit 14 Connect the heater and temp sensor of the replacement optical unit to their positions on the Temp and Motor control board 15 Connect the lamp wires to the VALVE LAMP terminal block 16 The replacement optical unit is now installed in the detector At this time turn on the detector Turn on should be normal If the LIGHT INTENSITY ERROR is on it will be necessary to re plumb the flow path so that the flow cell can be flushed to eliminate the LIGHT INTENSITY ERROR If the LIGHT INTENSITY ERROR is not on we will re plumb the flow path later Incorrect plumbing may destroy the flow cell Make
89. r 12 2001 NOTE Hardware Information Recorder Control Electronics Figure 23 on page 54 shows the electronic control circuits of the optical balance motor is a functional block diagram of the recorder control electronics All recorder outputs circuits reside on the AMPLIFIER board Some of these circuits are adjustable on the board The recorder output circuits are controlled by the recorder control circuits of the CONTROL board The RANGE switch sets the sensitivity of the detector signal voltage vs RIU Refer to the PERFORMANCE VERIFICATION section for details The POLARITY control changes the polarity of both recorder outputs from positive to negative The POLARITY INDICATOR led is ON for negative polarity This control does not affect the integrator output The RECORDER control when pushed to zero disables short to GND both recorder outputs This allows a strip chart recorder to be mechanically zeroed without disconnecting the detector signal cable The RECORDER indicator LED is ON for this mode Make sure that the RECORDER indicator LED is OFF for normal operation The MARKER button applies a 1 mV short duration offset to both recorder outputs This produces a deflection on the strip chart to mark the beginning of a chromatogram MARKER may also be initiated from the rear panel remote control inputs Service Handbook for 1047 Refractive Index Detector 12 2001 53 Hardware Information
90. rm Ckt e TP1102 Optical AB Board Con 115 Con 114 Con 601 Con 703 Course Zero O lt Input Row Balance Key Switch Board Output Board Service Handbook for 1047 Refractive Index Detector 12 2001 41 Hardware Information The motor can be moved either by the front panel and COURSE ZERO buttons or under certain conditions by initiating a BALANCE The OPTICAL AUTOBALANCE board produces the initial drive command pulses for the motor The correct frequency of these pulses 250 Hz 10 Hz is critical for proper operation of the motor Frequency can be measured at TP1101 normally LOW pulsing 250 Hz for motor operation and adjusted with VR2 The motor driver circuits of The TEMP AND MOTOR CONTROL board receive the drive command pulses from the OPTICAL AUTOBALANCE board On the TEMP AND MOTOR CONTROL board the operation of the motor driver circuits can be measured at TP204 normally LOW pulsing 250 Hz for motor operation TP205 should be constantly 5 VDC TP204 205 are near CON201 Finally the drive command pulses are converted to 12 VDC pulses and delivered to the windings of the motor The actual mechanical position of the motor is sensed by the motor position sensor potentiometer circuitry on the OPTICAL AUTOBALANCE board If the motor drives too far in either direction the front panel LIMIT ALARM in
91. se White from line fuse to left post of line filter Brown from bottom left post of LINE switch to transformer Black from top right post of LINE switch to line fuse Black from line fuse to right post of line filter Grn Yelllow to ground a a a a a N o on A ow N Red wire and Yellow wire un used 36 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Power Supply Wiring Figure 12 37 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 3 Hardware Information Motherboard Figure 13 shows the lay out of the motherboard Also shown is legend referred to in the cables termination table There are no like connectors on the motherboard This greatly reduces the possibility of connection errors Each individual connector is keyed so that it s cable may be inserted only in the correct way The PC board connectors are also keyed so that a board may not be inserted in the wrong location Be careful to note however that it is possible to remove these keys from the motherboard connectors Cables termination Term Description Motherboard holding screws six total To the REGULATOR SET mounted on the rear panel To the main power transformer secondaries connector To the AB MOTOR assembly To the VALVE LAMP terminal To the GROUND connector cable attached to the rear panel TEMPERATURE AND MOTOR CONTROL board POW
92. se the FINE ZERO dial to make the INT display as close to 0 00 as possible The detector output is now nulled Service Handbook for 1047 Refractive Index Detector 12 2001 93 NOTE Figure 39 Front Procedures Calibrate the LIGHT INTENSITY ERROR THRESHOLD 1 The detector output should still be nulled 2 Refer to Figure 39 on page 94 In the detector locate the CONTROL board Locate VR502 and TP2 on the CONTROL board On the CONTROL board the test points extend out from the rear of the board for easy access With a DC Voltmeter prepare to measure TP2 with respect to chassis GND The 20 VDC range is sufficient 3 Adjust VR502 so that TP2 reads 7 5 VDC The detector is now calibrated such that the LIGHT INTENSITY ERROR will come on if the current light in tensity as seen by the photo diodes deviates by approximately 25 from the original factory in tensity Location of Boards Lamp eos Amplifier Board Control Board VR VR VR 502 501 B04 0 Oo oO D TP2 TP1 TPA TPS 2 Rear 94 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Procedures Calibrate the Integrator Output Span 1 2 The detector output should still be nulled Locate the man
93. shown In older instruments a rotary voltage selector switch was used which employed four primary windings 100 V AC 115 V AC 220 V AC and 240 V AC In the current wiring scheme the 100 V AC wire red and the 220 V AC wire yel are not used These wires are capped and tied out of the way 32 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Mother Board n a C 1 Oo N Power Supply Board Figure 10 AC Power Distribution Mother Rear Panel Main r veel 1 Board Power Line ON OFF Power B 8VAC Tytoletl Con Receptacle Switch Transf P 2 10 and Line ransformer yellow z 0 Filter E 8VAC yellow 5 white white bro hit Neutral ak Lown c wns 5 Line Fuse E E Eer black pe white n Ground si 75 oe Orage SFOND j vac Hot gt A BVAC black black 240 z VAC blug black 8 E BVAC ae brown 9 Selector shown set for 115VAC 100 VAC x gray gray to CON 202 S hit pep ws Temp Control Board Part of l gt Temperature J orange s to CON 113 Control Circuitry EE Mother Board Rear Panel Temp ON OFF Switch Service Handbook for 1047 Refractive Index Detector 12 2001 33 Hardware Information DC Power Distribution Figure 11 provid
94. specification range range setting RecFS recorder full scale in mm For the following examples we assume a recorder FULL SCALE DEFLECTION of 250 mm and a correct detector recorder matching Verifying the Noise 1 Specification 2 3 Verifying the Drift Specification j i Noise Specification 2 5 x 10 RIU P P equivalent to 5 x 10 RIU P P 1047A RANGE setting to 1 64 x 10 RIU equivalent to 156 25 x 10 RIU Calculate 5 Noise oI RIU somm 8mm 156 25 x 10 RIU Allowable noise at range setting 1 64 is 8 mm P P Drift Specification 2 5 x 10 T RIU hr 1047A RANGE setting to 1 64 x 10 RIU equivalent to 1 5625 x 10 Calculate 2 5x 10 RIU Drift 5 1 5625 x 10 RIU x 250mm 400mm Allowable drift at range setting 1 64 is 40 cm hr or 7 cm over 10 minutes 106 Service Handbook for 1047 Refractive Index Detector 12 2001 Verifying the Wander Specification Example Calculating the noise Example Calculating the Drift Performance Verification Wander Specification 1 x 10 RIU equivalent to 2 x 10 8 RIU 1047A RANGE setting to 1 64 x 10 5 RIU equivalent to 15 625 x 10 Calculate 8 Wander AXU RIUS x 250mm 32mm 15 625 x 10 RIU Allowable wander at range setting 1 64 is 32 mm For the ChemStation In the ChemStation full scale range is set in pV 1 Oo Ne WO WD The 1047A integrator output 1 x 10 gt RIU 32000 pV is connected to th
95. specification M verifying the drift specification C1 verifying the wander specification e For the recorder outputs M the range knob and recorder outputs 1 the recorder full scale deflection setting m calculating recorder deflection CI verifying the noise specification M verifying the drift specification M verifying the wander specification e For the ChemStation m calculating noise and drift 100 Service Handbook for 1047 Refractive Index Detector 12 2001 Calculating Allowable Integrator Deflection Performance Verification 1047A Specifications Verification The specifications of the 1047A can be found in Specifications on page 15 For the Integrator Output The integrator output of the 1047A is calibrated such that 32 mV equals 1x 10 DELTA RIU Verifying 1047A specifications is a question of converting the specifications to an allowable deflection noise drift or wander on the integrator at a specific integrator attenuation The formula for calculating allowable deflection in mm at a specific integrator attenuation Spec Deflect eflection RIU mm where Spec specification of detector Deflection allowable deflection in mm RIU mm scaling factor at a specific attenuation See Table 6 on page 102 for the RIU scaling factor of Hewlett Packard integrators For other integrators refer to the manual for that integrator Look up the signal input required for FULL SCALE DEFLECTION at the specific
96. st been replaced If so has the protective clear tape around the cell been removed If not remove the tape See Lamp Turn Problems on page 113 Has the lamp just been replaced If so refer to the Lamp Replacement Intensity Adjustment on page 80 procedure Have any optical unit repairs just been made Review the calibrations that may be needed when repairs are made to the optical unit 1 CONTROL board 2 DIGITAL DISPLAY board Connect the ribbon cable securely 112 Service Handbook for 1047 Refractive Index Detector 12 2001 Table 9 Troubleshooting Information Lamp Turn Problems Lamp Turn Problems Possible Problem Actions The lamp does not turn on when the line switch is turned Lamp is defective Replace the lamp and perform the lamp on the detector itself turns on normally intensity adjustment Lamp voltage is absent Measure the DC voltage supply to the lamp wires at the valve lamp terminal Should be in the range of 2 5 to 3 0 VDC If the supply is absent 1 Replace POWER SUPPLY board 2 Replace POWER REGULATOR set Limit Alarm AB Motor Drive and Balance Problems These three areas are very closely related in the detector electronics The AB motor position is sensed by a feedback potentiometer which is connected to the motor shaft If the motor is driven too far in either direction the front panel LIMIT ALARM indicator is turned on From the LIMIT ALARM state the
97. sure that the flow path is plumbed as shown in Figure 29 on page 71 17 The original AB motor assembly may now be installed on the replacement optical unit Refer to AB Motor Assembly Replacement and Adjustment on page 73 Replace and adjust the AB motor assembly as described 18 Replace the left and right detector covers If you have not already done so re plumb the flow path Incorrect plumbing may destroy the flow cell Make sure that the flow path is plumbed as shown in Figure 29 on page 71 Turn on the detector and set detector temperature to 40 C After the detector has equilibrated at 40 C for 90 minutes perform the complete electrical calibration program described in Electrical Calibrations on page 93 Service Handbook for 1047 Refractive Index Detector 12 2001 79 NOTE Procedures Inside the Optical Unit Lamp Replacement Intensity Adjustment The replacement lamp assembly 01037 60002 consists of the bulb and it s holder The bulb is pre set in to the holder but the position of the bulb MAY need re adjustment as described in the intensity adjustment procedure below The replacement bulb should be cleaned with alcohol and clean cloth Care must be taken not to get fingerprints on the bulb 1 Remove the detector top cover If needed refer to Figure 40 on page 130 to identify the optical unit and lamp 2 Refer to Figure 44 on page 140 Remove the optical unit top cover item 13 insu
98. t Intensity Circuit 48 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Block Diagram Balance Not Ready Output Board Output Row gt Balance Not Ready Mother Board Cot Auto Balance Board Relay Driver Service Handbook for 1047 Refractive Index Detector 12 2001 49 Hardware Information The Inputs Balance A BALANCE is initiated by applying a short to the BALANCE pin pair or by the front panel BALANCE button Figure 20 Block Diagram Balance Input Output Board Balance Input Row lr 7 CON 703 Front Panel Front Panel Balance Balance Button LED CON 114 CON 115 Mother Y E Hl Board C Auto Balance Optical Auto Board Balance Board 50 Service Handbook for 1047 Refractive Index Detector 12 2001 NOTE Figure 21 Hardware Information INTEGRATOR POLARITY The INTEGRATOR POLARITY can only be changed by applying a short to the POLARITY pin pair Alternating shorts change INTEGRATOR POLARITY from plus to minus The current INTEGRATOR POLARITY is shown under the front panel digital display This is not to be confused with the record
99. tal Display Board 8 Range Switch Board Assembly 19 Holding Screws for Key Switch Board 9 Ribbon Cable to Motherboard CON116 20 Holding Screws for Display Switch Board 10 to Motherboard CON117 21 to Motherboard CON108 11 Fine Zero Adjust Assembly 22 to VALVE LAMP Terminal Block Service Handbook for 1047 Refractive Index Detector 12 2001 69 NOTE Procedures Use Figure 28 on page 69 as a guide for the replacement of a particular front panel component as described in the two generalized procedures below TEMPERATURE SELECT SWITCH assembly RANGE SWITCH BOARD assembly FINE ZERO ADJUST assembly or INTEGRATOR ADJUST assembly 1 Disconnect any cables associated with the assembly to be removed Refer to Figure 28 on page 69 for the locations of these cables Refer to Figure 42 on page 137 Working from the front remove the appropriate dial item 1 and nut The assembly may now be removed from the front panel Install the replacement assembly and re connect any cables associated with the assembly No calibrations are needed Re assemble the detector and check for proper operation LED BOARD 1 KEY SWITCH BOARD LED BOARD 2 DIGITAL DISPLAY BOARD or DISPLAY SWITCH BOARD Do not touch the position of any potentiometers Disconnect any cables associated with the board Refer to Figure 28 on page 69 for the locations of these cables Remove the screws which secure the board Figure 28 on page 69 and remove the bo
100. tc is eliminated Most problems in the RI system are chromatographic in nature and it is important to determine what contribution the liquid chromatograph is making to the problem NOTE Certain defects in the flush valve can resemble flow problems Refer to Flush Valve Problems on page 116 to see if your specific symptom is described NOTE Defects in the flow cell can resemble chromatographic problems Refer to Flow Cell Problems on page 121 to see if your specific symptom is described Refer to section 5 of the 1047A Operator s Handbook 01047 90000 for specific information Service Handbook for 1047 Refractive Index Detector 12 2001 125 Trou bleshooting Information Troubleshooting an Instable Baseline Excessive Noise 1 2 Make sure the optical unit locking screws are loose Make sure the laboratory bench is absolutely free of mechanical vibrations Make sure that lamp intensity is OK The average intensity TP1 and TP2 pressed together must be between 4 5 and 6 0 Values more negative than 6 0 can cause excessive noise Excessive Drift or Wander 1 The environmental temperature must be stable no open windows no excessive air conditioning or ventilation etc The column and column capillary must be shielded from temperature changes and excessive air flow Make sure that the flow cell stainless steel inlet outlet capillaries are installed correctly See Flow Cell Replace
101. tion b CON108 on the MOTHERBOARD This is the connection from the TEMP SELECT SWITCH assembly 2 Check the integrity of the rear panel TEMP switch 3 Check resistance through the temperature sensor if open replace the Optical Unit 4 Replace the TEMP AND MOTOR CONTROL board Service Handbook for 1047 Refractive Index Detector 12 2001 119 Troubleshooting Information Table 12 Temperature Control Problems Possible Problem Possible Causes and Actions 5 Replace the TEMP SELECT SWITCH assembly The front panel TEMPERA TURE indicator led turns on 1 Check the following connections for tightness normally but never begins to flash This means that the a CON202 on the TEMP AND MOTOR CONTROL board actual temperature as measured by the temp sensor This is the input for the 100 V AC heating power never reaches the setpoint b CON201 on the TEMP AND MOTOR CONTROL board This is the connection to the Optical Unit heater 2 Check the 2 A fuse on the TEMP AND MOTOR CONTROL board Replace fuse if open 3 Check for 100 V AC heater voltage at CON202 of the TEMP AND MOTOR CONTROL board This voltage should be steadily applied as long as both rear panel switches are on If voltage is absent check the PRIMARY and AC POWER DISTRIBUTION areas 4 Check resistance through the Optical Unit heater If open replace the Optical Unit 5 Replace the TEMPERATURE AND MOTOR CONTROL board 6 Replace the TEMP SELECT SWITCH assembly 7 The Opt
102. to Diodes Light beam Photodiodes As you adjust the mirror by hand use display TP1 and TP2 The goal of the mirror adjustment is twofold TP1 and TP2 should both be as high as possible and TP1 and TP2 must be as close together as possible Average intensity TP1 2 buttons pressed together must be no less than 3 5 but you should strive for the maximum intensity Typical intensities are from 4 0 to 5 5 When TP1 and TP2 are within 0 2 of each other you may use the mirror adjust glass Figure 32 on page 81 to make them equal DO NOT use the mirror adjust glass to compensate for a difference greater than 0 2 It could be helpful to slightly tighten the mirror holding screws then use a lever such as a small screwdriver or needle nose pliers to adjust the mirror This provides for a finer resolution of adjustment The mirror can move in several directions as shown in Figure 35 on page 87 This means that a very clear procedure for moving the mirror is not really possible You must find the mirror position which best meets the requirements of maximum intensity and photo diode equality Once the correct position for the mirror has been established tightening the holding screws can be a problem The position of the mirror tends to change as the screws are tightened To avoid this place your finger on top of the mirror and gently begin to press If the position of the mirror does not change TP1 2 unchanged then tighten the holdin
103. tting correct Are all cables in place and tight Measure test points on POWER SUPPLY board for correct output If test point measurements are NOT correct a Replace POWER SUPPLY board b Replace POWER REGULATOR set Light Intensity Problems When operating correctly the LIGHT INTENSITY ERROR indicator on the front panel will come on when the light intensity reaching the photo diodes differs from the original factory intensity by about 25 Ordinarily a LIGHT INTENSITY ERROR is caused by a reduction in intensity However an intensity increase as seen by the detector signal electronics will also cause the error The detector also gives a contact closure output rear panel while a LIGHT INTENSITY ERROR is present Actual light intensity can be displayed by TP1 sample photo diode and TP2 reference photo diode on the front panel Pressing TP1 and TP2 together will display the average in tensity of the two photo diodes The average intensity must be at least 3 5 Typical intensity values range from about 4 to 5 5 depending on optical unit cleanliness age and whether or not certain optical alignments were ever performed on site Service Handbook for 1047 Refractive Index Detector 12 2001 111 Troubleshooting Information Table 8 Light Intensity Problems Possible Problem Actions Air in flow cell this is the most common cause of the error In this case the intensity average as displayed by TP1 2 w
104. ual shaft turning knob on the front of the AB Motor assembly if needed refer to Figure 31 on page 74 As carefully as possible turn the AB Motor assembly shaft exactly TWO FULL TURNS in the COUNTER CLOCKWISE direction As the motor shaft is turned the INT display should be increasing in the positive direction Two counter clockwise turns of the shaft equals a beam displacement of 16 x 10 5 RIU Mechanically introducing a known RIU is more reliable and convenient than the classic method of preparing an RI solution for injection in to the cell Use Figure 39 on page 94 to locate the AMPLIFIER board On the AMPLIFIER board locate VR613 VR613 is clearly labeled on the board Adjust VR613 so that the INT display reads as close to 512 mV as possible The tolerance for this adjustment is 512 mV 10 The INTEGRA TOR OUTPUT SPAN is now calibrated such that 32 mV equals 1 x 10 RIU Calibrate the Recorder Output Span 1 The AB Motor shaft should still be adjusted to two full turns counter clockwise with the INT display reading 512 mV Set the front panel RANGE switch to 32 Select the REC display Locate VR612 on the AMPLIFIER board it is clearly labeled Adjust VR612 so that the REC display reads as close to 5 mV as possible The tolerance for this adjustment is 5 mV 10 Service Handbook for 1047 Refractive Index Detector 12 2001 95 NOTE Procedures Calibrate the Autobalance Decision Threshold 1 The AB Mot
105. ware Information Recorder Path The RECORDER signal path consists of the REC OUTPUT SPAN RANGE CONTROL MARKER OFFSET POLARITY CONTROL FS OUTPUT SELECT and REC SHOR TING CONTROL stages REC OUTPUT SPAN is adjusted by VR612 Figure 9 Output Board to Display from Elec Autobalance Board to Amplifier from Amplifier from Amplifier Board Board Board Recorder First Stage Integrator Circuits Circuits Output Row INT ImV 1OmvV Con 703 e o Out Z A 5 peses NJ GND lt 2 Resp Response 5 Time Time oO ou 5 Controler Output Board The RANGE CONTROL is set by the user from the front panel The MARKER OFFSET applies a voltage spike to the recorder signal for the purpose of marking the start of a chromatogram on a strip chart recorder The MARKER OFFSET can be applied manually from the front panel or automatically from rear panel 30 Service Handbook for 1047 Refractive Index Detector 12 2001 Hardware Information Recorder POLARITY can be changed only from the front panel FS OUTPUT SELECT is the beginning of the separate 1 mV and 10 mV full scale recorder outputs The REC SHORTING CONTROL provides an easy means of shorting zeroing the recorder outputs so that a strip chart recorder may be mechanically zeroed without disconnecting the detector The 1 mV recorder output is taken directly to the 1 mV recorder anal
106. y Board ERC75012 01047 66513 5 Preamp Board ERC75100H 01047 66502 6 Mother Board ERC75201A 01047 66508 7 Amplifier Board ERC75106A 01047 66507 8 Control Board ERC75105A 01047 66506 9 Auto Balance Board ERC57104A 01047 66505 10 Optical Auto Balance Board ERC75011A 01047 66501 11 Power Supply Board ERC75103A 01047 66504 12 Temperature and AB Motor Control Board ERC75102A 01047 66503 13 Output Board ERC75007B 01047 66515 14 Main Power Transformer 01047 61101 15 Lamp Assembly 01037 60002 16 Optical Balance Motor Assembly includes 01047 60016 complete assembly plus mounting bracket 17 Solenoid flush Valve Assembly 01047 60008 Service Handbook for 1047 Refractive Index Detector 12 2001 129 Parts Information Figure 40 Major Components Jeiector Rear 18 6 f 6 870 7 12 14 ww O 5 P NU Optical Unit 18 7 2 3 4 elector Front 130 Service Handbook for 1047 Refractive Index Detector 12 2001 Parts Information Major Assemblies The following table identifies the major assemblies shown in Figure 41 on page 134 Table 15 Major Assemblies Description ERC number Part number 1 Fro

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