63006320D Instruction Manual.pmd
Contents
1.2. LINKED TO CTS CTS 5 LINKED TO RTS DSR 6 OUTPUT FROM 6300 6320D DCD 8 OUTPUT FRM 6300 6320D DTR 20 INPUT TO 6300 6320D must be active GND 7 Suggested interconnections are detailed below 6300 6320D IBM PC XT 25 way D TXD 2 _ 2 TXD From PC RXD 3 3 RXD ToPC RTS 4 4 RTS From PC CTS5 5 CTS To PC DSR 6 6 DSR To PC DCD 8 8 DCD To PC DTR 20 20 DTR From PC GND 7 7 GND 6300 6320D IBM PC XT 9 way D TXD 2 _ 3 TXD From PC RXD 3 2 RXD To PC RTS 4 7 RTS From PC CTS5 8 CTS To PC DSR 6 6 DSR To PC DCD 8 1 DCD To PC DTR 20 4 DTR From PC GND 7 5 GND NOTE The Interface Cable Kit Order Code 542 009 can be used to implement the above interconnections Recorder Output This is available via the 4mm rear panel sockets The level is proportional to the displayed reading depending on the measurement mode Transmission 1mV per 0 1 T Absorbance 1mV per 0 001ABS Concentration 1mV per concentration unit 14 EC Declaration of Conformity The Buck 100 Spectrophotometer comply with the following European Standards EN 61326 1998 Electromagnetic compatibility Electrical equipment for measurement control and laboratory use EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Following the provision of EMC Directive 89 336 EEC and Low Voltage Directive 73 23 EEC 15
3. chamber lid is not closed 3 2 SAMPLE MEASUREMENT Many methodologies are well documented and where these are available the recommended wavelength should be used Where a new methodology is being developed the choice of wavelength can often be approximately determined by choosing an absorbance wavelength that is complimentary in colour to that of the standard solution The list given below illustrates this point The chart may be read from left to right or right to left i e a blue sample requires a yellow wavelength a yellow sample requires a blue wavelength Blue Yellow Greenish Blue Orange Bluish Green Red Green Red or Blue Colors as they relate to wavelength can be reviewed by placing a screen i e a piece of card in the light path where it passes through the sample chamber It will be seen that 400nm gives blue light and 700nm gives red with a progression between these points of green yellow and orange NOTE The human eye is unable to detect wavelengths below 400nm or above 700nm Once a complimentary colour has been chosen the precise analytical wavelength needs to be selected This wavelength will normally be that which gives the maximum absorbance value This can be selected by scanning in 5nm steps until an optimum is reached It should be noted that zero absorbance point will change with wavelength A zero CAL should be carried out whenever the wavelength is changed The complimentary color method of selecting
4. p acking case NOTE The instrument Monochromator is a non serviceable unit and no attempt should be made to repair this item Failure to observe this recommendation will result in the loss of any Warranty Claim on this product In the unlikely event of the monochromator requiring service or calibration it is essential that the Manufacturer or your local Distributor be contacted immediately for advice 4 2 LIGHT SOURCE REPLACEMENT The only routine maintenance which may be required is the replacement of the light source if this fails Failure should be suspected if the lamp failure indicator appears on the display This can be confirmed by looking in the sample chamber The Tungsten Halogen lamp is available from the Manufacturer or your local Distributor refer Section 5 2 Spares WARNING Disconnect the instrument from the mains supply prior to replacing the lamp Ensure the lamp is cool prior to handling Care should be taken when removing the lamp from the holder 1 Access to the tungsten halogen lamp can be gained via the lamp access p anel located at the rear of the unit refer Section 2 4 2 Slacken off the thumbscrew from the lamp access panel located at the rear of the instrument 3 Remove the old lamp from the holder The lamp is a plug in fit and should be removed by gently easing it from the holder IMPORTANT When fitting the replacement lamp it is essential that the glass envelope is not touched Finger marks will dam
5. Buck Scientific VIS 100 Operation Manual Safety This is important information please read carefully before installing or using this instrument 1 The Vis 100 spectrophotometer is designed for operation by trained personnel that are aware of the principles and applications involved For further help and advice please cont act us e mail sales bucksci com or visit www bucksci com 2 The Vis 100 spectrophotometer is a sensitive electronic and optical instrument designed for use in a laboratory environment Careful adherence to the inst allation instructions must be observed If in doubt contact a relevant and competent authority for advice before proceeding 3 In addition to observing the instructions detailed in the Operating Manual and Service Manual for this instrument all installation operating and service personnel must be aware of and employ a safe system of work 4 Voltage levels hazardous to life are present in this instrument for personal safety only trained engineers aware of the risk and avoidance of electric shock should remove protective covers from the instrument 5 This instrument is designed for minimal maintenance which must be carried out carefully following the procedures detailed in this manual All safety instructions in these procedures as well as those defined locally for the area or environment where the work is being carried out must be observed 6 Other than for those items defined in the mainten
6. acter regardless of the instrument operating mode For example 123 4 540 ASCII V lt CR gt Outputs a voltage proportional to the monochromatic light level passing through the sample and wavelength separated by an ASCII TAB character For example 1234 5 540 ASCII Z lt CR gt Calibrates a zero absorbance if the dark shutter is open SO lt CR gt command or zero transmittance if the dark shutter is closed SC lt CR gt command ASCII SC lt CR gt Closes the dark shutter which blocks monochromatic light entering the sample chamber This allows 0 transmittance to be calibrated ASCII SO lt CR gt Opens the dark shutter which allows monochromatic light to enter the sample chamber This allows 100 transmittance zero absorbance to be calibrated The shutter must be open for normal measurements ASCII Gnnn lt CR gt Commands the instrument to go to the wavelength nnm For example G540 lt CR gt will set the wavelength to 540nm 13 ASCII Fxxxx x lt CR gt Sets the concentration factor to xxxx x For example F1000 lt CR gt will set the factor to 1000 Note lt CR gt is an ASCII carriage return character The last three commands provide an output which can readily be incorporated into most spreadsheet software packages 6 2 RS232 Output The bi directional RS232 interface is available on the rear panel 25 way D type connector The connections are as follows TXD 2 INPUT TO 6300 6320D RXD 3 OUTPUT FROM 6300 6320D RTS 4
7. age the lamp Should accidental damage with finger marks occur the surface of the lamp may be cleaned using iso propyl alcohol 4 Carefully remove the replacement lamp from the packaging ensuring the glass portion of the lamp is not touched 5 Insert the lamp into the holder as illustrated ensuring that it is fully pushed home Fig 4 2 1 Lamp Fitting 6 Close the lamp access panel and re tighten the thumbscrew NOTE It is essential that only the specified replacement lamp is used Accuracy of optical alignment and performance cannot be guaranteed using alternative manufactured lamps SECTION 5 OPTIONAL ACCESSORIES 5 1 OPTIONAL ACCESSORIES The following list of items are available as optional accessories for use with the Vis 100 except where specified 630 005 630 020 630 022 630 021 633 001 632 001 632 031 630 028 635 100 543 001 542 009 033 290 5 2 SPARES 012 075 060 084 060 229 016 021 016 025 630 204 060 287 060 288 Adjustable Cell Holder 10 100mm Test Tube Holder 12mm Test Tube Holder 16mm Test Tube Holder 25mm Heated cuvette system cuvette holder and external control unit supplied with interconnection cables instruction manual mains cables for EU UK and USA Universal voltage and frequency External sipper pump supplied with inlet and outlet tubing mains cable and instruction manual 230V 50Hz External sipper pump supplied with inlet and outlet tubing mains cable a
8. ality deionised water or other suitable solvent must be used for dissolving or diluting samples chemicals and reagents All measurements require calibration to a blank for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample Where reagents are added to the sample to produce a colour proportional to its concentration a sample based blank should be used In this case the blank should consist of the sample plus any reagents or chemicals to be used except those that produce the colour to be measured Deviations from the Beer Lambert Law may occur at high and low concentrations giving non linear response during sample concentration measurements For all new methods a linear range should be defined by the preparation of a calibration curve Cuvettes and sample holders must be filled to a minimum level which covers the light path SECTION 4 MAINTENANCE 4 1 GENERAL The Vis 100 is designed to give optimum performance with minimum maintenance It is only necessary to keep the external surfaces clean and free from dust The sample area should always be kept clean and any accidental spillage should be wiped away immediately To give added protection when not in use the unit should be disconnected from the mains supply and covered with the optional dust cover 630 028 For longer term storage or re shipment it is recommended that the unit be returned to the original
9. ample Measurement Good Practice Guidelines MAINTENANCE General Light Source Replacement OPTIONAL ACCESSORIES Optional Accessories Spares INTERFACING Analogue RS232 N 2 1 2 2 2 4 2 5 2 6 4 1 4 2 5 1 5 2 6 1 6 2 SECTION 1 INTRODUCTION 1 1 INSTRUMENT DESCRIPTION The 100 is microprocessor controlled Visible Range Spectrophotometer covering the wavelength range of 320 to 1000nm with a 8nm bandwidth The monochromator is of a modified Czerny Turner design incorporating a stepper motor driven 1200 lines mm holo graphic diffraction grating and featuring automatic second order response suppression The 100 model has full interfacing capability for Analogue output and serial RS232 interfacing The optical system is independantly housed and isolated with lenses to give maximum protection from environmental contamination Combined with a mechanically rigid structure these models provide a system with fast warm up low drift and high reliability 1 2 INSTRUMENT SPECIFICATIONS Wavelength Range 320 1000nm Resolution inm Accuracy 2nm Bandwidth 10nm Transmittance Range 0 to 199 9 Resolution 0 1 Stray Light lt 0 5 Photometric Accuracy 2 Absorbance Range 0 300 to 1 999A Resolution 0 001A Concentration Range 300 to 1999 Concentration Resolution 0 1 1 Units ppm mg l g l M blank Factor 0 to 999 9 1000 to 9999 Photometric Noise Levels Photometric Stability Read
10. ance procedures herein there are no user serviceable items in this instrument Removal of covers and attempted adjustment or service by unqualified personnel will invalidate any warranty and incur additional charges for rep air 7 Reference should always be made to the Health and Safety Data for any chemicals or reagents used All available information advice and warnings on the handling storage use and disposal of such must be carefully observed When not available this data must be requested from the supplier before proceeding in any way 8 It is important that good laboratory practice is observed when handling samples chemicals reagents and ancillary equipment in order to carry out measurement and analysis with this instrument Suitable safety and personal protective equipment must be used at all times 9 Ifit is suspected that safety protection has been impaired in any way the spectrophotometer must be made inoperative and secured against any intended operation The fault condition must be reported to the appropriate servicing authority In all such reports model number and serial number of the spectrophotometer must be reported CONTENTS SECTION 1 SECTION 2 SECTION 3 SECTION 4 SECTION 5 SECTION 6 Vis 100 SPECTROPHOTOMETER OPERATING MANUAL INTRODUCTION Instrument Description Instrument Specification INSTALLATION Unpacking Installation Displays Controls Outputs Sampling OPERATION Initial Set Up S
11. ator is extin guished and the adjust annunciator is illuminated both primary and secondary display will show Pressing the RETURN or CAL key returns to concentration plus wavelength mode 3 3 GOOD PRACTICE GUIDELINES 1 For optimum performance all spectrophotometers should be sited in a clean dry dust free atmosphere When in use ambient temperature and light levels should remain as constant as possible Adherence to Standard Operating Procedures SOP and Good Laboratory Practice GLP should be monitored with regular calibration checks and a suitable Quality Control QC programme The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed The correct selection of sample containers is imperative for accurate and reproducible results a Plastic disposable cuvettes should be used ONCE only b Glass cuvettes should be thoroughly cleaned after use Discard when scratches become evident in optical surfaces c Care should be taken when selecting semi micro or micro cuvettes The cuvette window on the inner chamber the area filled with sample must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample In this case semi micro or micro cuvettes with self screening black surrounds must be used or alternative holders for these cuvettes fitted d Glass test tubes and other sample tubes should be used with car
12. ays ensure the fuse rating is correct Before attempting to change the voltage select disconnect the instrument from the mains sup ply Withdraw the fuse holder from the power input socket and remove the fuse Extract the grey fuse retainer and rotate so that the correct voltage is visible through the aperture in the fuse holder Replace the fuse retainer in its holder fit the correct fuse and push assembly back into the power input socket MAINS CONNECTIONS The unit is supplied with a moulded on plug However if this is removed for any reason the connection wires in the mains lead are colour coded to conform to the internationally recognised standard such that UK CONNECTIONS US CONNECTIONS BROWN LIVE BLACK LIVE BLUE NEUTRAL WHITE NEUTRAL GREEN YELLOW EARTH GREEN EARTH IMPORTANT THE UNIT MUST BE EARTHED The Green Yellow wire in the a c supply cable must be connected to a properly grounded terminal 2 3 DISPLAYS A 5 Mb UNITS A xF T ABS CONC A A 1 Primary display area Transmission Absorbance Concentration 2 Primary display adjust annunciator 3 Secondary display area Wavelength Factor 4 Primary display units 5 Secondary display adjust annunciator 6 Operation with PC 7 Menu options T ABS CONC FACTOR UNITS 8 Menu pointers for 7 2 4 CONTROLS Zz i 100 8 K 5 y FACTOR b aM VA CAL _ T ABS CONC xF UNITS AAA A
13. e Where possible matched tubes should be used and any index mark set to the correct position before measurements are made e Ensure any sample containers used are compatible with the consituents of both the samples and standards they are to hold Plastic cuvettes are not compatible with organic solvents f All sample containers must be handled with care by the top and non optical surfaces only Any finger marks evident must be removed by a suitable cleaning process g Flow through cuvettes must be selected with care and consideration for the sample type sample volume pumping system rinse sample and waste handling to be used Samples and standards should not be stored in open cuvettes or sample cont ainers as evaporation will change the value and lead to staining of the walls which may be irreversible If stored in stoppered and sealed cuvettes they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material Cold samples should be allowed to equilibrate to ambient temperature before measurement unless a suitable temperature controlled sample holder is in use Temperature change during measurement may cause air bubbles to form on the walls of the sample holder This is a common cause of drift during sample measurement 10 In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good qu
14. er vary so cannot be interchanged Calibrate on one and use only the same type of sample holder for each analysis batch SECTION 3 OPERATION 3 1 INITIAL SET UP NOTE If the unit is in continuous use it is recommended that it should be left switched on to obtain maximum lamp life Do not open the sample chamber door during initialisation Connect the unit to the correct mains supply and switch on After switch on the Vis 100 automatically re aligns the monochromator at zero order wavelength During this initialisation the instrument displays CAL on the primary display and the wavelength on the secondary display The wavelength will change as the instrument searches for the peak output at zero nm After calibration the unit will then go to the last wavelength used and enter the measurement mode last used The following error conditions which are displayed for 2 seconds are possible if the instrument fails to find the zero order peak Err 4 Lamp failed after initially finding Onm peak If the lamp has failed it should be replaced Err 5 No light level detected The instrument has failed to find a zero order peak Possible causes of this error are that the lamp has failed or that a sample has been left in the unit which is absorbing the zero order light Err 6 No dark level detected The instrument checks the region below 320nm which should have no optical throughput If this region cannot be found the most likely cause is that the sample
15. herefore independent of the solution in the light path The following error codes are possible after a calibration Err 1 Dark cal error This error occurs when the instrument closes the shutter to block light entering the sample chamber If the detector output does not fall to a level normally associated with a dark cal then this error is indicated The most likely cause is that the sample chamber lid is not closed Err 2 Light cal error This error indicates that there is insufficient light to calibrate to 100 The most likely cause is that light at the selected wavelength is being absorbed by a sample in the sample chamber This error can also be caused if the lamp has failed A calibration resets the sample number to unity on any printed results Absorbance Mode This mode is selected by moving the cursor to the ABS mode using the LEFT or RIGHT arrow keys refer diagram on p 4 The primary display will show the absorbance with ABS units The wavelength can be adjusted using the UP and DOWN arrow keys A calibration routine is initiated by pressing the CAL key The routine performs a zero transmission calibration followed by a 0 000 Absorbance calibration A blank clear solution should be used for this calibration procedure An internal shutter is automatically activated to perform the zero setting and this part of the routine is therefore independent of the solution in the light path The instrument will display overrange 1 ABS abso
16. nd instruction manual 110V 60Hz Dust Cover PC software on CD ROM compatible with Windows 98 and above 40 column printer Interface cable kit Storage case Tungsten Halogen Lamp 10 x 10mm plastic cuvettes pack 100 10 x 10mm plastic cuvettes pack 500 Replacement fuse 2A Replacement fuse 1A 10 x 10mm MkII Cell Holder Paper roll for printer Qty 1 Printer ribbon Qty 1 SECTION 6 INTERFACING Serial Interface The Vis 100 has a bi directional RS232 interface set to 1200 baud 7 data bits odd parity 1 stop bit The 25 way D connector allows a standard one to one interconnection lead to be used as supplied with the 40 column printer interface cable kit A printout is initiated by pressing the PRINT key If the sample munber is unity then the printout will include a header block The sample number is incremented every time the PRINT key is pressed The following commands can also be sent to the instrument via the serial interface using Windows HyperTerminal for example ASCII D ord Same as pressing the PRINT key ASCII T lt CR gt Outputs transmission and wavelength separated by an ASCII TAB character regardless of the instrument operating mode For example 100 0 540 ASCII A lt CR gt Outputs absorbance and wavelength separated by an ASCII TAB character regardless of the instrument operating mode For example 0 001 540 ASCII C lt CR gt Outputs concentration and wavelength separated by an ASCII TAB char
17. out lt 1 1 Hr after warm up Custom LCD Graphics display Outputs Analogue 0 1 999V d c RS232 serial port Light Source Tungsten Halogen 20W 12V Input Voltage 115 230Vac 20 10 Input Power lt 50W Size 365 w x 272 d x 160 h mm Weight 6Kgs SECTION 2 INSTALLATION 2 1 UNPACKING Remove the Vis 100 from the packaging and ensure the following items are present 1 Model Vis 100 Spectrophotometer 2 Mains Cable 3 Pack 100 disposable Cuvettes 060 084 4 Interface Cable 013 210 5 63 Zero Software 635 100 6 Optional Accessories as ordered Any shortages or damage should be reported immediately to the Manufacturer or your local Distributor 2 2 INSTALLATION MAINS SUPPLY The vias 100 model is designed to operate on 115 230V a c supply 20 10 50 60Hz The standard 2 metre mains cable supplied with the unit is fitted with an IEC type connector which can be plugged directly into the POWER IN socket on the rear panel The mains fuse is housed within the POWER IN socket When replacing the fuse the unit must be disconnected from the mains supply In the event of the fuse failing after replacement it is advisable to consult with the Manufacturer or your local Distributor before proceeding further Fuse Rating 2A F fast blow type NOTE The unit should be positioned within 1 5 metres of an earthed mains supply VOLTAGE SELECTION NOTE When changing the voltage select switch position alw
18. rbance during the dark calibration The possible error conditions are the same as for the transmission mode Concentration Mode This mode is selected by moving the cursor to the CONC menu option using the LEFT or RIGHT arrow keys The primary display will show the concentration with ppm mgt glt M or blank units The wavelength can be adjusted using the UP and DOWN arrow keys Pressing the CAL key places the instrument in a concentration cal mode indicated by the cal annunciator which allows the UP and DOWN arrow keys to be used to change the displayed concentration to the required value the wavelength adjust annunciator is extinguished and the primary adjust annunciator is illuminated Pressing the CAL or RETURN key with the required calibration standard in the sample chamber calibrates the concentration to the set value by calculating a multiplication factor for the current absorbance If the factor calculated is out of range then the instrument will display Err 3 for 2 seconds after the calibration The factor can be displayed on the secondary display by moving the cursor beneath the F AC TOR menu option With the factor displayed the UP and DOWN arrow keys allow the factor to be set directly Pressing the RETURN or CAL key returns to concentration plus wavelength mode The concentration units can be set by selecting the UNITS menu option and then scrolled through by using the UP and DOWN arrow keys the wavelength adjust annunci
19. wavelength may not be applicable in all situations perhaps because the solution has no distinct colour or the solution is of a complex nature and the absorbing species of interest is not the predominant colour In these circumstances it will be necessary to scan across the spectrum to determine points of maximum absorbance Situations also exist where solutions will absorb at more than one wavelength If this situation is encountered it is usually best to select the wavelength which gives maximum absorbance It is always good practice to verify linearity i e concentration vs reading of the methodology at the selected wavelength Where more than one absorbing wavelength is available it is prob able that one will offer better linearity characteristics than others To perform measurements the following procedures should be carried out Transmission Mode This mode is selected by moving the cursor to the T indicator on the menu bar using the LEFT or RIGHT arrow keys refer diagram p 4 The primary display will show the transmittance with T units The wavelength can be adjusted using the UP and DOWN arrow keys A calibration routine is initiated by pressing the CAL key The routine performs a zero transmission calibration followed by a 100 transmission calibration A blank clear solution should be used for this calibration procedure An internal shutter is automatically activated to perform the zero setting and this part of the routine is t
20. xe 1 used to adjust values on the selected display 2 used to move the cursor horizontally between menu options 3 used to select the displayed menu option 4 initiates a calibration routine 5 Print key Provides a printout of the current reading with an incremental sample number When pressed for the first time after a calibration the print out will give calibration information The incremental sample number will be reset after a calibration 2 5 INPUTS OUTPUTS Fig 2 5 1 Rear Panel Layout i x O RAE ni Disconnect poser before i 1 LAMP ACCESS PANEL This panel allows the user to gain access to the tungsten halogen lamp when replacement is necessary NOTE The Lamp Access Panel and all ventilation slots must not be covered or obstructed at any time 2 ROCKER SWITCH On Off switch for the unit 3 POWER IN SOCKET IEC type connection socket for mains cable 4 OUTPUT SOCKETS Analogue output 5 OUTPUT SOCKET Output socket for 25 way RS232 2 6 SAMPLING The Model VIS 100 is supplied with a 10mm cuvette holder Other options can be fitted refer to Section 5 Optional Accessories The Model VIS 100 has a dual 10mm cuvette holder with a 12 7mm test tube holder The domed lid on this model enables tall test tubes up to 100mm in height to be used WARNING The optical characteristics and light path of the cuvettes and test tubes used in the dual purpose sample hold
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