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1. 11 1 Preparation of new filter capsule The needed parts are The VOC filter two pieces of silicone tube sealing rings The two blue caps that are installed on the filter when unwrapped can be discarded Correct filter performance is crucial for the system performance e Filter element should be changed every 3 months Mark the date when it is put on and make sure to keep this interval User manual for G185 CO2 O2 flatbed incubator otart by putting the silicone tube sealing ring on each side of the new filter element It must be put on so that it touches the base of the filter fitting Press one end of the filter remember flow direction into the stationary side of the filter holder It should align fully with the base of the filter holder 19 User manual for G185 CO2 O2 flatbed incubator Then fit the other side of the filter into the sliding part of the filter holder and slide it into place It may be necessary to twist the filter element gently to get it to connect fully e Notice the flow direction on the side of the filter element indicated by an arrow on the label MUST be the same as shown on the side of G185 N A filter element that has been fitted incorrectly will cause the unit not to work as intended Filter correctly installed 20 User manual for G185 CO2 O2 flatbed incubator Flow direction as marked on the G185 11 2 Removing old filter The filter is removed by gen
2. JN Extreme care must be taken here as any wrong changes or adjustments will have a direct affect on the surface temperature of the Incubator 73 User manual for G185 CO2 O2 flatbed incubator 20 Gas level validation The gas level can be validated through the gas sample port on the backside of the 3185 or directly from each chamber using the test Lid 20 1 Gas level validation through gas sample port The gas level can be validated through the gas sample port located on the back side of the Incubator e Make sure that the Incubator is ON and the gas levels are stabilized before any gas measuring Remove the red Cap from the gas sample port and attach your gas analyzer to this port Make sure that there are no leaks on any tubing s gus NEVER ATTACH A PRESSURIZED GAS SOURCE OR ANYTHING ELSE THAT BLOWS INTO THE SYSTEM ON THE GAS SAMPLE PORT AN Taking out large sample volume may affect the gas regulation 74 User manual for G185 CO2 O2 flatbed incubator Remove the red protective cap hold carefully on to it Attach the end of tube of the gas measuring probe into the gas sample port Make sure that outside air is not drawn in The first measurement taken will be invalid as this is only the air inside the gas sample circuit that is being emptied Compare the CO readout with the value shown under CO2 C in the menu and adjust if necessary Please refer to chapter 17 3 for more instructions Compa
3. K SYSTEMS User Manual CO2 O2 Long Term Incubator G185 Version 1 6 User manual for G185 CO2 O2 flatbed incubator Table of contents General Intorination Gc Sori occisis tiaut io Oron bio sd E 5 2 Explanation of Symbols Used in Manual sess 6 2 LCL E E TE A ETE EA 7 4 MRC C AOI Wat IN OS age serach ots dcansciesoe sa atecteadoren ces erence a e a ERE aaa SE 10 SD Imporant Salety InsteictlOlly ucesseoisseptieu tu ek e ese RE ese Dope Rp S dia d xD daa EEEE 11 C enere Oea ES E E ee eee ee 12 ol e O e e E E E E 12 O FRC CCS SONICS SU ee E E eanocraie dood I2 5 9 CODUDSDUDOLH commen te PnP an Moreti e E aces Rn iM Uma Ce RED REDDUNT DUE 12 L Prono GO SOLFDLIODS eee et ee oe E d ertum piena Mito nta i nen ere 13 8 Control panel for G185 incubator lesssssesssesssssesessee eene nennen nnne nnns 14 Sd AOnpattuient MCIC ALOIS a arrested soretnanerteatotsanaatnapatencmcsutcaniveean ra De PED mu Rd PM SE Rus 14 MUCO eue ea 15 10 Cias connec ONS seeen m et 16 BM HEPAT YOC a E E E E AE RE 18 IL Preparation of new filter Capsule ipe Det pao treten eien 18 11 2 REMOVING DIO DIOE EEE dur E E E e ssp E 21 1 Io T 29 12 1 Activating the heat and gas controls ccceccceecccssecceeeeeeeesceeeeeceseeeeeeeeeseeceeeeseeeseenes 24 L TRC yO T Meo RE TE 25 IDEAE ENT mm 26 IC NES OT pros DU SIC E RR NE ETE T ETERNA 27 Dp IN pressure aldi uoo ect ido or
4. Therefore it is strongly recommended that in order to reduce the risk of electric shock the button cover not to be removed This equipment doesn t contain any serviceable parts It is therefore recommended to have qualified service personnel performing any service needed on the equipment There are several types of labels attached to a G185 A complete list of labels are shown on table below This device contains UV light with a wave length of 254nm Always power off by removing mains Jat Li power cord from socket before UV Light Hazard I amp vold looking removing cover directly at light N Exposure to UV radiation may cause serious damages to your skin and eyes Warning on the back of incubator indicates that an earth connection is needed This bolt of lightning indicates un insulated materials within your unit may cause an electrical shock For the safety of everyone in the laboratory please don t remove any covering Warning label on mains wire Main product label Warning for 110 115V countries Warranty label Warning The symbol indicates that the unit contains high sensitive electronic components which can be damaged by static electricity Internal label placed next to every earth wire K SYSTEMS brand logo User manual for G185 CO2 O2 flatbed incubator User manual for G185 CO2 O2 flatbed incubator Info labels on the back of the G185 di Always have red cap on un
5. the hours with the arrow down key Change the minutes from 00 59 in a similar way but this time using the arrow up key When the time is set correctly leave this menu point by letting go of the SP key and pressing the arrow up and down 54 User manual for G185 CO2 O2 flatbed incubator 17 14 St St The next menu point is St St This means Start Set and is where timer function is set to switch ON for the G185 Follow these steps to navigate in the St St option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option St St appears in the display Press and hold SP key to change values Change the hours 0 24 by pressing and holding the SP key and then pressing arrow down key Change the minutes from 00 59 in a similar way but this time using the arrow up key When the time is set correctly leave this menu point by letting go of the SP key and pressing the arrow down 55 User manual for G185 CO2 O2 flatbed incubator 17 15 Heat When the start time has been set the next menu item heat must be switched to ON if the warmed area should warm up Follow these steps to navigate in the HEAT option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option hEAt appears in the display Press and hol
6. 17 Hour The HOUR option gives the opportunity to show the time on the display when the heat is off Follow these steps to navigate in the HOUR option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press arrow DOWN key until the option HOUR appears in the display Press and hold SP key to change values While holding the SP key in press either the arrow UP or arrow DOWN key to select between ON o r OFF When the required setting is activated let go of the SP key Press and hold UP DOWN key for 3 seconds again to exit the menu lt is also possible to toggle between the temperature display and the clock by pressing SP and heat w Remember to press SP first as you otherwise will switch off the unit Press and hold SP key While holding the SP key in press the HEAT key once then let go of the SP key The display now shows the time User manual for G185 CO2 O2 flatbed incubator 17 18 UV light The next item in the menu is u1 This is the toggle ON OFF for the UV light inside the circuit The UV light will make sure that no contamination occurs in the circuit When correctly set up there is full Carbon filtering of the air so that any harmful by product from the UV light process is removed at all times If the UV light is not needed it can be switched off from the menu Please follow below instruction on how t
7. CO or wrong gas Check gas supply make attached to CO gas input sure that 1 0 bar of gas pressure is applied Actual gas concentration Check CO set point at is higher than set point CO SP in the menu Poor CO gas regulation Lid s are left open Close lid s Insulation missing on lid s Check the grey insulation on every lid CO2 L is shown on CO gas concentration Allow system to stabilize display more than 1 from set by closing all lids point CO Pr is shown on No wrong CO gas Check CO gas supply display pressure to system make sure that pressure is kept stable at 1 0 bar 84 User manual for G185 CO2 O2 flatbed incubator O gas regulator No Oz gas regulation System not powered Vee is on standby or Switch the system on MEN eai off O gas regulator is OFF Activate O gas regulator by setting O2 r to ON in the menu No N2 or wrong gas type Check gas supply make attached to N gas input sure that 1 0 bar of N gas is applied EE gas concentration Check Oz set point at BEEN ES than set point O2 SP in e menu Poor O gas regulation Lid s are left open Close lid s Closelids an missing on lid s bogota muli the oy insulation on ever bogota muli MEM L is shown on display Os gas concentration more Allow system to stabilize than 71 from set point by closing all lids n2 Pr is shown on No wrong Ne gas pressu
8. In the UNIT option the displayed temperature can be set either to degrees Celsius or Fahrenheit As default this is set to C Follow these steps to navigate in the UNIT option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press and hold SP key to see if C or F is chosen While holding the SP key in press either the arrow UP or arrow DOWN key to toggle between Celsius C or Fahrenheith F Press and hold UP DOWN key for 3 seconds again to exit the menu 34 User manual for G185 CO2 O2 flatbed incubator 17 2 CO2 concentration Setpoint Co SP is the CO set point This option is used to change the desired CO concentration in the system In order to change the CO setpoint please follow below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press arrow DOWN key until the option Co SP appears in the display Press and hold the SP key to see the current set value for CO concentration Change by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e As default the CO setpoint CO SP is set to 5 0 35 User manual for G185 CO2 O2 flatbed incubator 17 3 CO concentration calibration Co2 C is the COs calibration or a reading of the actual CO concentration in t
9. UNIT will appear in the display Press DOWN key until the option Co2 r appears in the display If CO regulation is not needed it can be switched OFF here The Default value is ON CO regulation is switched to OFF by holding the SP key while pressing the arrow DOWN key once When the CO regulation has been set for OFF there will not be shown any value under the CO2 C menu point It will display OFF to indicate that the regulator has been deactivated by user Press and hold UP DOWN key for 3 seconds to exit the menu Always connect 100 pure CO source to the CO gas inlet located on the back of G185 before switching the regulator ON 37 User manual for G185 CO2 O2 flatbed incubator 17 5 Oxygen concentration setpoint O2 SP is the O2 set point This option is used to change the desired O2 concentration in the system In order to change the O setpoint please follow below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option o2 SP appears in the display Press and hold the SP key to see the set value Change by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e As default the O setpoint O2 SP is set to 5 096 38 User manual for G185 CO2 O2 flatbed incubator 17 6 Oxygen C
10. Chamber no 5 and 10 Chamber no 4 and 9 Chamber no 3 and 8 Chamber no 2 and 7 Chamber no 1 and 6 Big comparent 71 User manual for G185 CO2 O2 flatbed incubator Please follow these steps for a detailed instruction of how to validate calibrate the temperature within each zone 1 Place warming plate in all 10 chambers 2 Switch on the incubator and wait for at least 40 minutes for the temperature to stabilize at set eg 37 0 degrees 3 Open lid no 10 tn 1 and place the dish containing oil based liquid and temperature sensor inside 4 Place test Lid and pull the sensor wire through the hole on the test Lid Wait for the temperature to get stabilized When temperature is stabilized write down the value and move on to the next chamber within the same zone User manual for G185 CO2 O2 flatbed incubator 5 When the temperature of both of chambers within the same zone has been measured the average value must be calculated using the following formula in Xx Templ Temp2 Temp avr E LUE 7 EM where x is the number of zone 6 Go through step 3 and 5 and calculate the average temperature value for all 6 zones When all 6 values are calculated they should be compared with what is being shown by the system This is done by entering the menu and checking the values for tn 1 to tn 6 Adjust if the calculated value are different then the ones displayed by the system for each zone
11. ailed description of temperature measurement on G185 52 User manual for G185 CO2 O2 flatbed incubator 17 12 Regulation factor The INT T Integral Time option is for changing the base value for the PID controller This should not be attempted by unauthorised persons If set at a different level the controlling principle will be affected Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option int appears on the display Pressing and holding the SP key will give a reading of the current setting The value for regulation factor can be change using SP key along with arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e From the factory it is set to a closely calculated value specific for the model 2 35 Do not change the value here unless authorization has been given directly by K SYSTEMS 53 User manual for G185 CO2 O2 flatbed incubator 17 13 Timer setting The ti St refers to the timer functions which means time set Follow these steps to navigate in the Ti St option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option Ti St appears on the display Press and hold SP key to change values Change the hour 0 24 by pressing and holding the SP key and then changing
12. connector can either get connected to a voltage source OR to a current source N Note that if a current source is attached to the 2 5mm jack connector the maximum current rating is between 0 1 0 Amp N If a voltage source is attached then the limitation is between 0 50V AC or DC 80 User manual for G185 CO2 O2 flatbed incubator If there is no alarm the switch within the unit will be in ON position as it is illustrated No Alarm mode below 2 5mm jack connector Whenever the G185 goes into an alarm mode the switch will become an open circuit This means that no current is able to run through the system anymore dc T REED RSE vse Beene pikes ir D pot B 2 5mm jack connector e Whenever the incubator power cord is removed from power outlet in the wall this switch will automatically indicate an alarm This is an extra safety feature added to alert the personnel in case of power cut out to the laboratory 81 User manual for G185 CO2 O2 flatbed incubator 23 Writing pads for the compartment lids and penholder Magnetic writing pads can be put on each compartment lids so that patient data or content of the compartment be written down Only write with a suitable non toxic pen that allows the text to be removed afterwards There is a pen holder located on the right hand side of the C185 82 User manual for G185 CO2 O2 flatbed incubator 24 Maintenance Reliable and safe opera
13. contain any user serviceable parts Please leave all maintenance work to qualified personnel 6 1 Setup precautions In order to avoid any possible damages to this system please follow all setup precautions described in this chapter Finding a suitable location Place this unit on a flat hard and stable surface Never place the unit on a carpet or similar surfaces Never place this unit on top of other equipment that might heat it up Space for ventilation Never put anything under this unit this might block the ventilation openings causing possible damage to system Place this unit in a location with adequate ventilation to prevent internal heat build up Allow at least 10 cm clearance from the rear 30 cm from the top and 20 cm from left and right to prevent overheating Avoid high temperature moisture water and dust This unit must not be exposed to dripping or splashing This unit is intended for indoor purposes only 6 2 Accessories supplied e 3 VOC filter capsules plus one installed e 2 HEPA filter for input gas supply e 10 warming blocks for Nunc OR Falcon e 10 magnet pads to be attached to lids for writing amp Pack of Pens e 1 CD ROM containing K SYSTEMS A S data logging software e 1 Data Extension Cord amp 1 USB Converter e 2 Silicone Tube Sealing Rings amp Silicone Tube 3 m e Test Lid e 1 CD ROM containing USB converter drivers 6 3 Copyright notice The making of any unauthorized copies of copy protected
14. d 25 User manual for G185 CO2 O2 flatbed incubator 14 Alarms When the C185 is in an alarm mode a message will be shown on the display The below table contain a list of the alarm messages and an explanation of the alarms CO2 pressure alarm CO2 level alarm N2 pressure alarm O2 level alarm In the following chapters these alarm states are explained in more details 26 User manual for G185 CO2 O2 flatbed incubator 14 1 CO pressure alarm If the CO gas supply is not attached correctly or wrong CO gas pressure is applied to the system the G185 will go into CO pressure alarm mode CO2 P will be displayed indicating wrong incoming gas pressure CO gas supply failure e P stands for pressure A constant audible alarm is also activated which can be muted by pressing the alarm button e By pressing the alarm button 6 the CO2 P message on the display will also disappear but the red LED light will stay on until correct gas pressure is applied to unit User manual for G185 CO2 O2 flatbed incubator 14 2 N2 pressure alarm If the N gas supply is not attached correctly or wrong N gas pressure is applied to the unit the G185 will go into alarm mode n2 P will be displayed indicating wrong incoming nitrogen gas pressure The gas supply is not attached correctly or empty nll P stands for pressure A constant audible alarm is also activated which can be mute
15. d SP key to change values While holding the SP key in press the arrow UP to select between ON or OFF When the required setting is activated let go of the SP key Press and hold UP DOWN key for 3 seconds again to exit the menu If the Heat is set to off when the timer function is set nothing will be switched on when the timer engages Make sure that all compartments are empty when the timer function is used 56 User manual for G185 CO2 O2 flatbed incubator 17 16 A St The A St Automatic Start option is used to repeat the timer St St function everyday of the week Follow these steps to navigate in the A St option Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option A St appears in the display Press and hold SP key to change values While holding the SP key in press the arrow UP to toggle between ON or OFF When the required setting is activated let go of the SP and arrow keys Press and hold UP DOWN key for 3 seconds again to exit the menu Penultimate menu point for the timer function is A St This function will activate the timer all days if toggled to ON and the time start time has been set accordingly e Remember there are no calendar functions in the timer so all days include Saturday and Sunday 57 User manual for G185 CO2 O2 flatbed incubator 17
16. d by pressing the alarm button s By pressing the alarm button 6 the N2 P message on the display will disappear but the red LED light will stay ON until correct gas pressure is applied to unit 28 User manual for G185 CO2 O2 flatbed incubator 14 3 CO level alarm CO level alarm is activated if the concentration of the CO gas is deviating by than 196 from set value Remember when changing the set point more than 1 from the current gas level this will result in an CO level alarm The same goes for all calibration adjustments Gas concentration is deviating by more than one percent from set value e When there is an alarm an acoustic signal is also activated which can be muted by pressing the alarm Cu 14 4 O gt level alarm O2 level alarm is activated if the concentration of the O gas is deviating by more than 196 from set value Remember when changing the set point more than 1 from the current gas level this will result in an CO level alarm The same goes for all calibration adjustments O gas concentration is deviating by more than one percent from set value e When there is an alarm an acoustic signal is also activated which can be muted by pressing the alarm Cu 20 User manual for G185 CO2 O2 flatbed incubator 14 5 Temperature alarm All zones of the heating system can trigger a temperature alarm if they are more than 0 5 degree C from the set point Remember w
17. display Press arrow DOWN key until the option REST appears in the display Press and hold SP key to change values While holding the SP key in press either the arrow UP or arrow DOWN key When the display shows let go of the SP key Press and hold UP DOWN keys for 3 seconds again to exit the menu A complete restore will be done 63 User manual for G185 CO2 O2 flatbed incubator 17 23 Logic firmware The logics installed on your G185 is upgradeable Whenever an important update is available it will be distributed to our distributers around the world who will make sure that your incubator runs with the newest available logic firmware Please follow these steps to check the logic firmware which is currently installed on your unit Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option uEr appears in the display Press and hold SP key to check the unit s current firmware version Press and hold UP DOWN key for 3 seconds again to exit the menu 64 User manual for G185 CO2 O2 flatbed incubator 18 Front chamber keys On the sloping front of the G185 there are 10 buttons located They are numbered from 1 to 10 Each button corresponds to one compartment The compartments will be labelled to show which specific compartment corresponds to which button Should the labels have been remov
18. e O regulator must be switched to ON Follow the below instructions to activate deactivate the O2 regulator Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option o2 r appears on the display When the O regulation has been set for OFF there will not be shown any value under the 02 C menu point It will display OFF to indicate that the regulator has been deactivated by user Press and hold UP DOWN key for 3 seconds again to exit the menu Always connect a 100 pure N source to the gas inlet on the rear before switching the regulation ON The Default status for the oxygen control is OFF 40 User manual for G185 CO2 O2 flatbed incubator 17 8 CO2 flow rate Co2 F is the CO gas consumption It is automatically measured by the system and the actual consumption is shown In order to get a reading of current CO gas consumption please follow below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option Co2 F appears in the display Pressing and holding the SP key will give a reading of the actual flow rate Press and hold UP DOWN key for 3 seconds again to exit the menu e CO gas consumption is not a fixed value and may change in the intervals from 0 l h 10 l h The n
19. e iene taccetncug tee eceenincdaten 54 GAA C m S 55 INS EE 56 EA A e earcmendcavseceeceosd 57 mE qr 58 INE MORE 59 1719 Pressure Of 1icomilp CO Pas isis sioaaoe teneat bua aii 3400 qutbE io n700300500000 029902955 2020908 520 000855 60 17 20 Sores ie OF mCi IN5 D OS oo esquequ iuuat aotem E E 61 WES MM B CON TA AO NR m mm 62 VN MEI 63 IS MU UTE TAR AEC IO DOES DR 64 Lo Pron t chambef OS atin actresses AE MpI ORUM Me tom Ret 65 19 WSS ay AN OM see ceat bot bir oo SPP ca Uerum ees epe et ixi vun rh inque domus ES 70 DOTS devel valo AERO A EA deer ahis D E Roo Mesue VUE Dep A AE E oNtue poma Egbr 74 20 1 Gas level validation through gas sample port sess 74 20 2 Gas level validation through each gas chamber esee 76 PANE eee ILU INN m m 79 22 Alarm switch for external dialling system eeesssessseeeeeseneennem enne 80 23 Writing pads for the compartment lids and penholder 0 0 0 ecceceeeeceeseeeceeeeeeeeeeeeeeeees 92 3 User manual for G185 CO2 O2 flatbed incubator P M Ese NER TE E TE S E TEE AE E EA AEE E E E 83 25 OE SION a a E E EE A 84 87 26 Technical aSsistance cccccececccc
20. e zone2 Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option tn 2 appears on the display Pressing and holding the SP key will give a reading of the current temperature of zone 2 chamber no 4 and 9 Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e tn 2 is used to adjust the temperature of compartment 4 and 9 e Adjust according to a high precision measurement done in a dish with media under mineral oil located in compartment 4 and 9 e Please refer to chapter 19 for a detailed description of temperature measurement on G185 48 User manual for G185 CO2 O2 flatbed incubator 17 11 3 Calibration of Zone 3 The item called Tn 3 in the menu is used to calibrate the temperature in the zones Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option tn 3 appears on the display Pressing and holding the SP key will give a reading of the current temperature of zone 3 chamber no 3 and 8 Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e tn 3 is used to adjust the t
21. ececccceceueccececencecececcececeecenessceneneenseeneenenessseseseeessenenes User manual for G185 CO2 O2 flatbed incubator 1 General Information amp Service COPYRIGHT This manual contains information that is subject to copyright All rights reserved This manual should not be photocopied otherwise copied or distributed completely or in part without the approval of K SYSTEMS Kivec Biotec Users of K SYSTEMS Kivex Biotec products should not hesitate to contact us if there are any unclear points or ambiguities in this manual K SYSTEMS Kivex Biotec A S Klinteho vaenge 3 5 3460 Birkerod Denmark Tel 45 45 99 56 00 Fax 45 45 99 56 19 E mail info K SYSTEMS dk Internet www K SYSTEMS dk Service address Please contact your local K SYSTEMS Kivex Biotec distributor for details CAUTION If the equipment is used in a manner not specified by this manual the safety of the user may be at risk and the equipment may be damaged Always use the equipment as outlined in this instruction manual User manual for G185 CO2 O2 flatbed incubator 2 Explanation of Symbols Used in Manual oe NOTE Used to direct attention to a specific item WARNING Used to warn the user against a specific item DANGER Used when caution is needed HELP Used when the help of an assistant is needed User manual for G185 CO2 O2 flatbed incubator 3 Safety symbols The G185 contains high voltage components that may be hazardous
22. ecessary amount needed is automatically calculated and adjusted by the system oe Remember that 100 pure and dry CO gas will bee needed to increase the CO level in the system 4 User manual for G185 CO2 O2 flatbed incubator 17 9 N2 flow rate n2 F is the No gas consumption The necessary amount is automatically calculated and adjusted by the system and the actual consumption is then shown In order to get a reading of current N gas consumption please follow below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option n2 F appears in the display Pressing and holding the SP key will give a reading of the current nitrogen consumption Press and hold UP DOWN key for 3 seconds again to exit the menu e N gas consumption is not a fixed value and may change in the intervals from 0 l h 54 l h The necessary amount is automatically calculated and adjusted by the system oe Remember that 100 pure and dry No will bee needed to reduce the Oz level in the system Remember also to activate the Oz regulation as described in chapter 17 7 42 User manual for G185 CO2 O2 flatbed incubator 17 10 PC PDA data logger The next item in the menu is r232 This is the communication protocol with the PDA or a Windows based PC The communication can be toggled between PC PDA or OFF here Press and h
23. ed the order is also listed below 65 User manual for G185 CO2 O2 flatbed incubator Pressing a key will toggle the light in the key on off This light can be used as an indication warning that the compartment is in use and should not be opened Never leave a compartment lid open for unnecessary long time Each opening should be minimized to less than 10 seconds under normal use Inside the compartment there is a warmed area that covers the bottom In the middle the gas intake and outlet is located This must be kept free at all times If the gas inlet outlet in the middle of each compartment is blocked the gas concentration in that compartment will no longer be correct The area in each chamber can be cleaned with a suitable cleaning detergent If alcohol or a similar embryo harmful substance is used make sure that the lid is kept open for some hours afterwards or rinse with sterile water so that all traces are removed before use User manual for G185 CO2 O2 flatbed incubator The gas in outlet of the compartment is shown in detail Insert the warming optimization plate The warming optimization plate will ensure full contact with the dish This generally means much more stabile temperature for the cells 67 User manual for G185 CO2 O2 flatbed incubator The plate fits the compartment The plate can be taken out and put in an autoclave for cleaning Place the dish where it fits the pattern There i
24. emperature of compartment 3 and 8 e Adjust according to a high precision measurement done in a dish with media under mineral oil located in compartment 3 and 8 e Please refer to chapter 19 for a detailed description of temperature measurement on G185 49 User manual for G185 CO2 O2 flatbed incubator 17 11 4 Calibration of Zone 4 The item called Tn 4 in the menu is used to calibrate the temperature in the zone4 Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option tn 4 appears in the display Pressing and holding the SP key will give a reading of the current temperature of zone 4 chamber no 2 and 7 Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e tn 4 is used to adjust the temperature of compartment 2 and 7 e Adjust according to a high precision measurement done in a dish with media under mineral oil located in compartment 2 and 7 Please refer to chapter 19 for a detailed description of temperature measurement on G185 50 User manual for G185 CO2 O2 flatbed incubator 17 11 5 Calibration of Zone 5 The item called Tn 5 in the menu is used to calibrate the temperature in the zoned Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT wi
25. en culture may lead to evaporation and a change in pH External dialler is an option Please contact your K SYSTEMS sales representative in your country for more information 13 User manual for G185 CO2 O2 flatbed incubator 8 Control panel for G185 incubator In the below table the control panel is explained Alarm button is used to mute an audible alarm Standby On Turns on this unit or turns off to normal standby mode Display panel Shows information about the current status of the unit Setpoint s used to select item in the menu in order to change their status It is also used to change temperature and gas setpoints Arrow keys up and down Are used to navigate through the menu and changes values for temperature and gas concentrations 8 1 Compartment indicators The compartment indicators are used to indicate whether a compartment is occupied or not 14 User manual for G185 CO2 O2 flatbed incubator 9 Mains connection The G185 comes with a non detachable mains power cord The power cord is prepared for the country in which the unit is intended to be used in Insert this in a suited mains supply that is grounded Do not defeat the safety purpose of the grounding type plug A grounding type plug has two blades and a third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet The
26. he system Remember that the CO regulator must be activated before the concentration can be viewed or changed Whenever an adjustment of the CO concentration is needed it can be done here by following below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option Co2 C appears in the display Press and hold the SP key to have a reading of the current CO volume Use SP key and arrow UP or DOWN to adjust the reading based on reliable data from an external gas analyzer Press and hold UP DOWN key for 3 seconds again to exit the menu e Please refer to next chapter to for instructions on how to activate CO regulator e Please refer to chapter 20 for more details on gas level validation and calibration procedures e Calibration is done by adjusting the CO level according to a measurement done on the gas sampling outlet using a precision CO2 measurement device only N Changing the calibration value should only be done based on certain measurement by a trained user or a technician 17 4 CO2 regulator User manual for G185 CO2 O2 flatbed incubator CO2 r is used to toggle the COs regulation ON OFF As default the CO regulator is set to ON Follow the below instructions to activate deactivate the CO regulator Press and hold UP DOWN key for 3 seconds to enter the menu The option
27. hen changing the set point more than 0 5 degrees from the current temperature this will result in an alarm The same goes for all calibration adjustments The alarm condition is indicated by a red LED light in the alarm key and a pulsating audio signal that can be muted by pressing the alarm key once To mute the alarm sound press once Press once again and the sound will no longer be muted e When there is a temperature alarm an acoustic signal is also activated which can be muted by pressing the alarm outon e The display will not change in any way and will stay at the point where it was when the alarm started 30 User manual for G185 CO2 O2 flatbed incubator 15 Setting the temperature The set temperature is 37 0 C by default The set temperature can be adjusted in the range from ambient to 49 0 C Changing the Set point for the temperature follow these instructions Press the button with the symbol SP the current set temperature will be shown on the display To raise the set temperature press and hold SP then press arrow UP When the desired Set point is reached release both keys To lower the Set point temperature press and hold SP then press arrow DOWN When the desired set point is reached release both keys e If SP and arrow UP or DOWN are pressed continuously the temperature setting will change quickly To change the temperature slowly press one step at a time 31 User manual f
28. ibed in the manuals e To reduce the risk of fire or electric shock this equipment should not be exposed to rain or moistures and objects filled with liquids 10 User manual for G185 CO2 O2 flatbed incubator 5 Important Safety Instructions e Read the safety instructions before using equipment e Always keep these instructions e Heed all warnings e Follow all instructions e Do not use this apparatus near water e Do not block any ventilation openings e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Do not defeat the safety purpose of the grounding type plug A grounding type plug has two blades and a third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet e Protect the power cord from being walked on or pinched particularly at plugs convenience receptacles and the point where they exit from apparatus e Unplug this apparatus during lightning storms or when unused for a long period of time e Refer all servicing to qualified service personnel servicing is required according to service manual or if the apparatus has been damaged in any way such as if the apparatus have been dropped exposed to rain or moisture or does not operate normally 11 User manual for G185 CO2 O2 flatbed incubator 6 General description N This unit does not
29. ime to adjust Ce Remember that if one warmed area is much warmer than the area in its proximity there will be cross over heating Calibration is best done by starting out with Tn 1 and then Tn 2 to Tn 6 Then letting the system stabilize and repeating the procedure Please refer to chapter 19 for a more detailed description of temperature validation 46 User manual for G185 CO2 O2 flatbed incubator 17 11 1 Calibration of Zone 1 The item called Tn 1 in the menu is used to calibrate the temperature in zonet Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option tn 1 appears on the display Pressing and holding the SP key will give a reading of the current temperature of zone 1 chamber no 5 and 10 Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e tn 1 is used to adjust the temperature of compartment 5 and 10 e Adjust according to a high precision measurement done in a dish with media under mineral oil located in compartment 5 and 10 e Please refer to chapter 19 for a detailed description of temperature measurement on G185 47 User manual for G185 CO2 O2 flatbed incubator 17 11 2 Calibration of Zone 2 The item called Tn 2 in the menu is used to calibrate the temperature in th
30. is described There are 6 separate controls for the temperature on the G185 Each controller is responsible in controlling the temperature of a separate zone Therefore each of the 6 available zones is equipped with its own separate temperature sensor and heater allowing the user to adjust the temperature within every zone separately and achieve higher precision Each zone can be calibrated separately using the item that corresponds to the respective zone in the menu These items are placed in the menu and they are named Tn 1 Tn 2 Tn 3 Tn 4 Tn 5 and Tn 6 An overview of the zones associated with the tune functions is shown on table below Big compartment 1 User manual for G185 CO2 O2 flatbed incubator From the table above we can see that compartment no 5 and 10 is controlled by Tn In order to adjust calibrated the temperature of this zone please find item called tn 1 and adjust using high precision thermometer AN AN Calibration of temperature is done by adjusting the tn x where x is the zone number according to a measurement done on both of the chambers within the zone by a precision Thermometer After temperature adjustment allow at least 15 minutes for the temperature to stabilized use the thermometer to verify correct temperatures on each zone Be careful when changing the calibration settings to only change the tune value of the place where the measurement is actually done Give the system t
31. ll appear in the display Press DOWN key until the option tn 5 appears on the display Pressing and holding the SP key will give a reading of the current temperature of zone 5 chamber no 1 and 6 Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e tn 4 is used to adjust the temperature of compartment 1 and 6 e Adjust according to a high precision measurement done in a dish with media under mineral oil located in compartment 1 and 6 e Please refer to chapter 19 for a detailed description of temperature measurement on G185 51 User manual for G185 CO2 O2 flatbed incubator 17 11 6 Calibration of Zone 6 The item called Tn 6 in the menu is used to calibrate the large compartment used for dry bath incubation purposes Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option tn 6 appears on the display Pressing and holding the SP key will give a reading of the current temperature of zone 6 Large compartment Change according to a precision thermometer by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu e The large compartment is only warmed and has no gas control Please refer to chapter 19 for a det
32. material including manuals and software is an infringement of copy rights and constitute a criminal offence User manual for G185 CO2 O2 flatbed incubator 7 Product descriptions The G185 incubator is a new generation of desktop CO2 and O2 incubator used for maintaining an optimal environment for the development of embryos and blastocysts Direct warming of the dishes in the chambers gives superior temperature conditions in comparison to conventional incubators The G185 incubator is composed of 10 separate culture heat chambers each having its own lid and a flat warming block insert The warming blocks are customized to accommodate several types of dishes for either Falcon or Nunc In addition the G185 incubator has a large warmed compartment on the side that can hold culture media For maximum performance the system has 6 separate temperature controllers controlling and regulating the temperature in the culture chambers and the large compartment The Incubator needs 10096 CO2 and 10096 N2 in order to be able to control the CO2 and O2 concentrations in the culture chambers The incubator has been developed and optimized for tissue culturing with an overlay of either Paraffin or mineral oil The incubator can be connected to a pc running the K SYSTEMS data logging software for long term data logging and data storage Also an external dialler can be connected to the incubator to ensure maximum safety and reliability Op
33. n the gas bottle This is because there is a built in pressure limiter in the circuit 60 User manual for G185 CO2 O2 flatbed incubator 17 20 Pressure of incoming N gas The next item in the menu is n2 Pr and stands for N pressure This function can only be used to monitor the N gas pressure inside the unit In order to check the internal N gas pressure please follow these instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option n2 Pr appears in the display Press and hold SP key to check the actual N gas pressure normally between 0 35 0 65 Press and hold UP DOWN key for 3 seconds again to exit the menu By pressing the SP button the actual N gas pressure is shown normally between 0 35 0 65 e Remember that the inside pressure is always lower than what is shown on the outside regulator on the gas bottle This is because there is a built in pressure limiter in the circuit 61 User manual for G185 CO2 O2 flatbed incubator 17 21 Decontamination The next item in the menu is dCnt which stands for decontamination This function enables the user to heat up the whole heated area zone 1 to zone 6 up to 60 C for approx 1 hour in order to decontaminate the whole heated surface The decontamination system is equipped with a timer that automatically stops the decontamination process
34. o switch ON or OFF for the integrated UV light Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press arrow DOWN key until the option u1 appears in the display Press and hold SP key to see actual status Press and hold SP while pressing arrow DOWN once to change status of UV light Press and hold UP DOWN key for 3 seconds again to exit the menu The UV light will automatically switch off when the unit is turned off e For optimal air purification reasons it is recommended to have the UV light set to ON when unit is being used 59 User manual for G185 CO2 O2 flatbed incubator 17 19 Pressure of incoming CO gas The next item in the menu is Co Pr and stands for CO2 pressure This function can only be used to monitor the CO gas pressure inside the unit In order to check the internal CO gas pressure please follow these instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press arrow DOWN key until the option Co Pr appears in the display Press and hold SP key to monitor internal COs gas pressure Press and hold UP DOWN key for 3 seconds again to exit the menu The actual CO gas pressure in the circuit is shown normally between 0 35 0 65 e Remember that the inside pressure is always lower than what is shown on the outside regulator o
35. old UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option r323 appears in the display Press key UP or DOWN once while holding the SP to turn on the communication port Data logging is prepared and sent for a PC default When the required setting is set let go of the SP key Hold the SP button and press down once this will change the display reading to PDA Now data are being prepared and send for a PDA attached to the C185 Hold the SP button and press down once more this will change the display reading to OFF No data is being sent to the communication port Press and hold UP DOWN key for 3 seconds again to exit the menu User manual for G185 CO2 O2 flatbed incubator Having the parameter set incorrectly when no PDA or PC is attached will not make any difference Only switching to OFF when the PDA or PC is attached will impact on the datalogger software Communication protocol is set to PC by default Please refer to the enclosed Installation instruction for datalogger software for further instruction for datalogger installation Never attempt to switch off the dataflow when the PDA or PC is logging data Stop the program first on the PDA or the PC 44 User manual for G185 CO2 O2 flatbed incubator 17 11 Surface Temperatures In this section the temperature controls of the G185
36. oncentration Calibration The item called O2 C is found in the menu and is used for calibration or just having a readout of the actual O concentration in the system Remember that O regulator must be activated before the concentration can be shown changed Whenever an adjustment of the Os concentration is needed it can be done here by following below instructions Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option o2 C appears in the display Reading the actual O value is done by pressing and holding the SP key Change by pressing and holding the SP key and arrow UP or DOWN Press and hold UP DOWN key for 3 seconds again to exit the menu Please refer to next chapter to for instructions on how to activate Os regulator Please refer to chapter 20 for more details on gas level validation and calibration procedures e Calibration is done by adjusting the Os level according to a measurement done on the gas sampling outlet by a precision O2 measurement device N Changing the calibration value should only be done based on certain measurement by a trained user or a technician User manual for G185 CO2 O2 flatbed incubator 17 7 O2 regulator O2 r is used to toggle the O regulation ON OFF If Os regulation is not needed the oxygen regulator can be switched off here If low oxygen conditions are needed th
37. or G185 CO2 O2 flatbed incubator 16 Menu Function The user can access a number of advanced functions via the menu Please follow the below instructions for entering exiting and navigating within the menu Press and hold UP DOWN key for 3 seconds to enter the menu The first option UNIT will appear in the display Press UP DOWN key to navigate within the Menu While holding the setpoint key in press either the arrow UP or arrow DOWN key to change values within the options Press and hold UP DOWN key for 3 seconds again to exit the menu 32 17 Overview of the menu items CO SP Set Point for CO CO2 C CO concen tration CO2 regulator O2 SP Os Set Point O2 C O concen tration O2 r O regulator CO2 F CO flow n2 F N flow r232 Data logging Continue on next column rf LN t tn 1 Calibration of Zone 1 tn 2 Calibration of Zone 1 tn 3 Calibration of Zone 3 tn 4 Calibration of Zone 4 tn 5 Calibration of Zone 5 tn 6 Calibration of Zone 6 int t Regulation factor ti st Time setting st st Start set Continue on next column User manual for G185 CO2 O2 flatbed incubator heat Timer for heater A set Auto set hour Display time u 1 Uv light CO pr COs pressure n2 pr N pressure dc nt Decontami nation uer Current logic version User manual for G185 CO2 O2 flatbed incubator 17 1 Unit
38. power requirement is 230V 50 Hz OR 115V 60Hz and must be specified by customer prior to order NAM AS Connecting the unit to a wrong voltage will cause serious damages to the system 15 User manual for G185 CO2 O2 flatbed incubator 10 Gas connections On the back of the unit there are two gas inlets and one gas sample port outlet These ports are marked CO2 N2 and Gas sample port The CO2 inlet should be connected to 100 pure CO2 The N2 inlet should be connected to 10096 pure N2 if low oxygen conditions are required O2 control in the compartments is available in the range from 20 2 by infusing the N2 e Gas pressure for both inlets should be 1 0 bar 14 5 PSI and must be kept stable Always use a high quality pressure regulator for both gases 16 User manual for G185 CO2 O2 flatbed incubator Remove the red cap for the gas inlet that is needed Take care not to loose the cap as it will be needed when gas is not connected to the unit Connect the CO2 gas to the CO2 inlet with a suitable silicone tube Connect the N2 inlet to the Nitrogen Bottle in a similar way e If no Oxygen control is needed leave the red cap on the N2 inlet N Never run the unit with an inlet or outlet NOT covered by a red cap 17 User manual for G185 CO2 O2 flatbed incubator 11 HEPA VOC filter The combined HEPA and VOC filter is mounted on the side of the unit for easy access and replacement
39. re Check No gas supply display to system make sure that pressure is stable at 1 0 bar If Oo regulation is not needed set the O2 r to OFF in the menu to deactivate oxygen regulation and abort the N alarm Data logger No data is send to PC System not powered System is on standby or owitch the system on switched off The rs232 item in menu Change to PC is set to OFF or PDA mode Incubator and PC not use the cable supplied oroperly attached along with unit Data logger software USB Please refer to software driver not properly installation guide for installed instructions MEM Data cable between Check connection Only 85 User manual for G185 CO2 O2 flatbed incubator Display Missing segment s in Failure in the PCB Replace the PCB Contact display your K SYSTEMS Distributor for details Keyboard Absent or erratic function Failure in the keyboard Replace keyboard of operation buttons Contact your K SYSTEMS Distributor for details 86 User manual for G185 CO2 O2 flatbed incubator 26 Technical assistance For more information contact the K SYSTEMS office nearest you For Denmark call 45 45995600 Outside Denmark see your K SYSTEMS catalogue for the phone number of the office nearest you or visit our website at www K SYSTEMS dk for up to date world wide contact information K SYSTEMS is pleased to provide internet access to product information for i
40. re the N2 readout with the value shown under O2 C in the menu and adjust if necessary Please refer to chapter 17 6 for more instructions e Remember to put the protective red cap on the outlet afterwards User manual for G185 CO2 O2 flatbed incubator 20 2 Gas level validation through each gas chamber The gas level can also be validated by taking a small gas sample from each of the chambers AN K SYSTEMS strongly recommend the use of K SYSTEMS gas analyzer as this unit is specially developed to take samples from small size chambers without interfering the internal gas conditions within each chamber Make sure that the Incubator is ON and the gas levels are stabilized before any gas measuring Prior to any gas measuring make sure that the Lid have not been opened for at least 5 minutes Make sure that the gas analyzer is calibrated before use Please follow these instructions for gas measuring directly from each chamber 1 Make sure that the system is ON and that the gas levels are reached and stabilized 2 Make sure that no Lids are opened through the whole validation process 3 Inject the needle from the gas analyzer through the chamber and make a gas measurement 76 User manual for G185 CO2 O2 flatbed incubator 4 Test Lid can also be used for customers using other types of gas analyzers Compare the CO readout with the value shown under CO2 C in the menu and adjust if necessar
41. s a warming optimization plate for Nunc and for Falcon dishes The plate is marked with a N on the reverse side for Nunc and F for Falcon type dishes A little mineral oil inside the groove will make it easier to remove the dish 68 User manual for G185 CO2 O2 flatbed incubator N The K SYSTEMS warming blocks for the G85 is not recommended for use in the G185 69 User manual for G185 CO2 O2 flatbed incubator 19 Temperature validation The temperature of each of the 6 zones can be validated using the following equipment In order to be able to perform the temperature test the following items are needed e Precision thermometer e Falcon or Nunc dish with integrated PT 100 sensor mounted in the dish e Oil based Liquid e Test Lid 70 User manual for G185 CO2 O2 flatbed incubator There are 6 different regulators on board a G185 5 regulators for controlling the surface temperature of the chambers tn 1 tn 5 and 1 controller for the big compartment tn 6 Within every zone there are 2 chambers In order to be able to make an accurate temperature validation it is strongly recommended to measure the temperature on both of the chambers whiting the specific zone eg To validate the temperature of tn 1 the temperature of chamber no 5 and chamber 10 must be measured and an average value calculated The below table is used to localize the chambers associated with the specific zones
42. te iIIa ocrepa iiu tUe dise ce eincaseniuesoces 28 CERSEI AE a secs E wa css ae cow psn ees 29 14 4 O level alafM eee ae ne ee 29 Id Temperature alai Mesoni Ea a a aa ES 30 I Se UN UNS Tee T e a E E EE 31 PGs E AT a o POE E E N ate E AE E E ET E ne A 32 I7 OVERVIEW of the The nu AGING cs scien cisnervanetendsaselersemntndecannGaisaantine Dev asap 2 saben 2M a cup riase 33 MEN UUM E 34 172 COZ 6Ooncentratiot SCO eeke eed Hoppe On pa oca fcre toten dd taico E ad Edad 35 17 3 CO concentration calibration 20 0 0 cece cece ec ecececccccccececccccececececececececscececececectctaneees 36 User manual for G185 CO2 O2 flatbed incubator CA CO Pe EAU a E 37 PS Oxygen concentration setpPO Disse E E E 38 17 6 Oxygen Concentration Calibration ccccccccccsseccceeceeesceceseceaeseeeeeeeaeeesseeseeeeeeenes 39 IW M PATET EROR T 40 EF KG ivo Tc C 4 are MS PAQUETE mo o 42 LIO WC PPG aa NO SC Ci 2 9999299999929 12 99999998999 800 0 80 90959 99 0005 52 50 9995 2 0 3585 43 ILLE Suttace Tempe NUN eno rat E Ros aper Mc OMNE UMEN 45 LLI Caipaton or Zone Tessio Edad esepuDEN Mu NUI SS UM PI MM MUM 47 KATMA S nong WA C 48 EES C OnO ZOMG a E E E AE N 49 IW OW OL AOE aar E E 50 IWAN NEG sciet 7 ONC dT r R E TE E EE E 51 I AIG Calibration of Zone E 52 IEEE PAN TOLLIT ME LS CU ERR 53 Mee T 66 UNS ease canta deaste aa ncace seed asetenaeaeccsebeor da yereaseaceuseeac
43. tion of this equipment is based on the following conditions 1 Correct calibration of temperature and gas using high precision equipments in the intervals described in the manuals 2 Replacement of VOC and inline HEPA filters in the correct intervals specified in the manuals 3 Inspection and replacement of UV light according to the intervals defined in the manuals 4 Replacement of Oxygen sensor in the correct intervals defined in the manuals N It is strongly recommended to perform the inspection and servicing in the intervals described in the MAINTENANCE and SERVICING manuals Refusing to do so can have fatal outcome causing the unit to stop performing as expected and cause damage to Embryos Blastocysts etc that are being kept alive inside the incubator N Guarantee void if service and maintenance are not followed 83 User manual for G185 CO2 O2 flatbed incubator 25 Troubleshooting Heating svstem No heating display is off The heating switch is off Press the heating switch No heating The alarm is on The temperature is more than 0 5 C off the set temperature EEEKARM C temperature is wrong temperature set point Heating not even System not calibrated Calibrate each zone according to the user manual using high precision thermometer CO gas requlator No CO gas regulation System not powered EE eod is on standby or owitch the system on off by setting CO2 r to ON in menu No
44. tly rotation the filter element with one hand and pulling back the sliding part of the filter holder 21 User manual for G185 CO2 O2 flatbed incubator N Never run the G185 with the filter element missing 22 User manual for G185 CO2 O2 flatbed incubator 12 Menu In the next chapters the functions associated with the keyboard and the menu items explained The table below shows the main keys and their purposes Alarm button is used to mute an audible alarm Standby On Turns on this unit or turns off to normal standby mode Display panel Shows information about the current status of the unit Setpoint s used to select item in the menu in order to change their status It is also used to change temperature and gas setpoints Arrow keys up and down Are used to navigate through the menu and changes values for temperature and gas concentrations 23 User manual for G185 CO2 O2 flatbed incubator 12 1 Activating the heat and gas controls The heat and gas controls are activated using the heat 6 button soon after system activation the main display will alternate the reading between the following 3 parameters Tp Temperature in C or F C is default Co2 C CO Concentration in 96 O2 C O Concentration in 96 This is illustrated in the below table Tp Temperature in C or F default is C After to has been shown the actual temperature will be displa
45. ts products To obtain user guides and any other documents that may associate with this product please go to the K SYSTEMS website at hitp www k SYS TEMS dk 214 products htm All goods and services are sold subject to the terms and conditions of sale of the company within K SYSTEMS which supplies them A copy of these terms and conditions is available only on request Contact your local K system representative for the most current information 2008 K SYSTEMS All rights reserved K SYSTEMS A S Klintehoej Vaenge 3 5 DK 3460 Birkeroed Denmark KIVEX BIOTEC LTD Phone 45 45995600 Fax 45 45995619 Web K SYSTEMS dk 87
46. used gas input and outputs Chamber numbers on the front of the incubator can be seen in the left corner of each chamber Label on the large heating compartment Please note that there is no gas control in the large compartment The large compartment is not intended for any long term incubation purposes User manual for G185 CO2 O2 flatbed incubator 4 Precautions warnings e Do not use product if package is damaged e Do not use product without a proper VOC filter attached e Do not use this product at temperatures exceeding 30 C e Do not block the cooling fan while in operation e Never use a non K SYSTEMS filter e Use only 100 pure CO2 and 100 pure N2 gas e Always use HEPA filter for input CO2 and N2 gas e Always keep red cap on unused gas and sample lines e Read and understand the user manual completely before use e NEVER block gas supply pins placed in the middle of each of the 10 gas chambers e Make sure that CO2 and N2 gas supply pressures are kept stable at 1 0 bar e Always connect power cord to a proper grounded outlet e VOC filters must be changed in the interval described in the manuals e UV lamp must be changed in the interval described in the manuals e O2 sensor must be changed in the interval described in the manuals e Big compartment only to be used to keep things warmed e Never leave lids open for more than 10 seconds e Perform temperature and gas calibration in the intervals descr
47. when the desired temperature has been reached and brings the system back to normal status AS WHILE DECONTAMINATION PROCESS IS ACTIVE ALL GAS REGULATIONS ARE DEACTIVATED AN ACOUSTIC ALARM WILL WARN THE USER ABOUT RISING TEMPERATURES THE ACTUAL TEMPERATURE AND DECOMINATION TIME LEFT WILL BE CONSTANTLY BEE SHOWN ON THE DISPLAY THE SYSTEM WILL ALSO WARN THE USER ABOUT THE HOT SURFACE TEMPERATURES To activate the decontamination process please follow the instructions below Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the display Press DOWN key until the option dCnt appears in the display Hold the SP button while pressing arrow UP or DOWN buttons to toggle the decontamination OFF or bring it back ON again Press and hold UP DOWN key for 3 seconds again to exit the menu aS AFTER END DECONTAMINATION PROCESS THE UNIT WILL STILL BE HOT DO NOT USE THE DEVICE UNTIL THE TEMPERATURE AND GAS LEVELS ARE BACK TO NORMAL IT IS RECOMMENDED TO TEST THE TEMPERATURE AND GAS CONCENTRATIONS BEFORE USE TO ENSURE CORRECT LEVELS User manual for G185 CO2 O2 flatbed incubator 17 22 Reset Next item in the menu is the function REST This is the overall back to factory default settings function To restore factory settings please follow the instruction below Press and hold UP DOWN key for 3 seconds to enter the menu The option UNIT will appear in the
48. y Please refer to chapter 17 3 for more instructions Compare the N readout with the value shown under O2 C in the menu and adjust if necessary Please refer to chapter 17 6 for more instructions e Remember to put the protective red cap on the outlet afterwards J4 User manual for G185 CO2 O2 flatbed incubator a li E rem Sf mna emer M Teta There is no gas control in the big compartment so all bottles kept in this compartment should be sealed or not be sensitive to CO2 or O2 levels 78 User manual for G185 CO2 O2 flatbed incubator 21 AC cooling fan The cooling fan is placed on the button cover of the G185 and is used to cool down the electronics inside N The ventilation holes under the G185 must be kept clear at all times 79 User manual for G185 CO2 O2 flatbed incubator 22 Alarm switch for external dialling system In order to be able to connect the G185 to an external monitoring system for maximum safety especially during the nights and weekends this Incubator is equipped with an external connector a 2 5mm jack connector in the back which can be connected to such a monitoring device Whenever there is an alarm that could be temperature alarm out of set point gas alarms for CO or Os levels low pressure alarms for CO and Ne gases no gas alarms or if the power to the unit suddenly is cut the switch is de activated indicating that the unit needs inspection by a user The
49. yed for a few seconds Co2 C CO Concentration in 96 After CO2 C has been shown the actual CO gas concentration will be displayed for a few seconds O2 C O Concentration in 96 After O2 C has been shown the actual O gas concentration will be displayed for a few seconds If CO regulation is deactivated the system will show OFF right after CO2 C has been displayed If O regulator is deactivated the system will display OFF right after O2 C has been displayed Activation of CO2 and O2 controls require that the CO regulator CO2 r and O regulator O2 r have been activated within the menu 24 User manual for G185 CO2 O2 flatbed incubator 13 Keyboard Lock In order to prevent any change of the settings by mistake the G185 has a built in keyboard lock that can be activated de activated using the keyboard buttons Please follow the following instructions to lock and un lock the keyboard Press arrow up and arrow down along with the alarm key at the same time will lock the keyboard When pressing a random key the display will show lock while keyboard is locked To unlock the keyboard press SP along with Arrow up and down keys at the same time e If the keyboard lock has been activated and any buttons is pressed Lock will be shown on the display to indicate that the keyboard is locked e Kindly note that the alarm still can be muted when key lock is engage

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