INSTRUCTION MANUAL SCl 10 SPEEDVAC? CONCENTRATOR
Contents
1. INSTRUCTION MANUAL SC110 SPEEDVAC CONCENTRATOR SC110A SC210A SPEEDVAC PLUS CONCENTRATORS y SAVANT INSTRUMENTS INC 100 Colin Drive Holbrook NY 11741 4306 1 800 634 8886 516 244 2929 Fax 516 244 0606 111 3001 REV 8 3 94 TABLE OF CONTENTS TABLE OF CONTENTO 3ixs6deveskNuV aut ssYesuivus dquxes c dens oto demel ius Gao E E Siss Es ORI oa 2 10 BESCRIPTION queste cider io ienasi eL QI RINSSE ER PERI IER Spese ua e 3 2 0 INSTALLATION i kotvek sexitenai EUR IN IR TEX TAE UM TN V VER VES 3 Receiving soarce iis teal rud e Ue er ERE Eze b Nie ERAN EPRAN UE RS ODER ARV NNERAE RN CRAB ROCA 3 MOM RD TP 4 Inspection iore t nee e eai EH EM a RH URHRVEDKUH T sane 4 Bet MD oisi tue E v EYE SURE RU Iu UN o CAN VRAE LEM x CeEN A MUN ERN VEA RAN RUM HE YE 4 3 07 OPERATION 2 5 deeethest eecepeelopen Piera Serres areko iv tea dn Sd Vue tic da 5 3 1 COONIROLPANEL 52 iid ehe uS Hh d inedotisoratendonsvete sts peendsnarncensehvays 5 3 2 ege m 6 3 3 PREHEATING iscccsvescvsscancsssnciessterssdenskssaccudevepuscona des sy eavets a ve 6 34 SPEEDVAC SC110 OPERATION eere eere eene eene enn eren eene nne T 3 5 SPEEDVAC PLUS SC110A SC210A OPERATION 7 3 6 OPERATION WITH2. 088 6019 00 088 6020 00 M40 0022 01 095 1041 00 M60 0002 01 095 1061 00 M90 0074 00 095 6064 00 095 6004 00 ORS100 M60 0005 01 M60 0005 02 E60 0006 01 M60 0006 01 M50 0023 01 095 1058 00 095 1059 00 095 1005 00 M50 0020 01 095 1007 00 095 6053 00 M20 0001 01 095 6005 00 M80 0002 01 090 1031 01 090 1031 02 E70 0004 06 E70 0020 01 095 1057 00 095 1060 00 M60 0044 02 This part is not applicable to the 110 13 C210A 090 6012 00 087 6050 00 088 6020 00 M40 0003 01 095 1041 00 M60 0002 01 095 1061 00 M90 0074 00 90 600 1 095 6006 00 ORS200 M60 0015 01 M60 0015 02 E60 0006 00 M60 0006 01 M50 0023 01 090 1021 00 090 1022 00 095 1005 00 M50 0020 01 090 1003 00 95 670 2 M20 0001 01 095 6005 00 M80 0002 01 090 1031 03 090 1031 04 E70 0004 06 E70 0020 01 095 1057 00 095 1060 00 M60 0044 02
3. solenoid vacuum is applied manually using a Savant bleeder valve The SC110A and SC210A models differ only in size The SC110 and SC110A hold smaller rotors some of which hold up to 100 sample tubes The SC210A holds larger rotors and can accomodate up to 200 tubes A refrigerated trap is always used with the apparatus to protect the vacuum pump but this is not important in servicing the concentrator 1 2 Theory of Operation The concentrators have two modes of operation o Manual mode The operator activates rotation and chamber heating using the switches on the concentrator s front panel On the 110 the operator operates a manual bleeder valve at the top of the concentrator and must sequence the application of rotation and vacuum manually On the SC110A SC210A an internal vacuum solenoid operates autonatically o Slave mode SC110A SC210A The user attaches a Savant 1021 controller to the 9 pin connector at the rear of the unit and connects the sample chamber vacuum line to the controller The controller operates the rotor and gives the user many options for automatic or timed operation However the front panel DRYING RATE switch still controls the concentrator s chamber heater A circuit board inside the concentrator contains relays by which the unit rotates or brakes the rotor controls the chamber heater and activates the internal vacuum solenoid SC110A SC210A only LEDs monitor the state of each relay
4. 3001 REV B 3 94 3 6 OPERATION WITH THE SC1021 SPEEDVAC SYSTEM CONTROLLER With vacuum tubing connect the hose fitting at the rear of the SpeedVac concentrator to the other instruments as described in the SC1021 instruc tion manual Move the SpeedVac CONCENTRATOR switch to the ON position and leave it there the SC1021 will operate its motor The CONCENTRATOR switch can be shut OFF as an emergency stop for the rotor The DRYING RATE switch will still control the concentrator s chamber heater Remove the small metal plate at the rear of the concentrator cabinet SC110A SC210A only exposing the 9 pin connector Attach the control cable from the SC1021 Plug the line cord into the SC1021 The text display of the SC1021 gives you step by step guidance The SC1021 operates the concentrator sequences rotation and application of vacuum automatically ends the run according to your specifications and provides advanced features such as stored programs and run statistics Refer to the SC1021 instruction manual for details SPECIFICATIONS Dimensions W x H x D SC110 SC110A 11 4inx 13 0inx 17 6 in 29 0 cm x 33 0 cm x 44 5 cm SC210A 18 3 in x 18 1 in x 25 3 in 46 5 cm x 46 0 cm x 64 1 cm Weight SC110 SC110A 36 Ibs 16 kg SC210A 97 lbs 44 kg Electrical Requirements SC110 SC110A 120 V AC 60 Hz 6A or 240 V AC 50 Hz 3A SC210A 120 V AC 60 Hz 8A or 240 V AC 5OHz 4A Cabinet Chemical resistant coated steel constructi
5. Magnet Assembly seems normal do not reinstall the Upper Magnet Assembly yet The assembled unit can fail even though failures are not evident in the disassembled pieces This is because when assembled the upper and lower magnets pull toward each other A failure or misadjustment of the set screws on the Lower Magnet Assembly can impede rotation only when the unit is assembled Close the chamber cover and switch the ROTOR switch to ON without the Upper Magnet Assembly installed If the motor rotates or rotates quietly when this was not the case before then unplug the unit remove the bottom plate and remove the four screws that secure the motor mounting bracket Adjust the two 38 32 set screws so that the drive magnet is exactly 1 16 from the stainless steel closure plate This plate covers the hole to the vacuum chamber While the unit is apart verify that switching the ROTOR switch ON turns the LED labeled MOTOR on and the one labeled BRAKE off This verifies that the circuit board is trying to rotate the rotor and that the brake is off If these LEDs light inappropriately return the circuit board to Savant indicating a logic error in rotor rotation or braking as the case may be Reassemble the unit If the motor is slow or noisy even when the Upper Magnet Assembly is removed then replace the motor and Lower Magnet Assembly 2 8 Vacuum Problems If the apparatus fails to achieve or maintain vacuum and if the fault is traced t
6. SC110s and SC110As configured for 220VAC and all SC210As have a cover lock driven from the board without using a relay this line is also monitored by an LED A PAL integrated circuit on the board ensures correct operation in each of the operating modes 1 3 Sequence of Operation Each device in the controller can be considered separately Rotor The concentrator can select rotation or braking Rotation is enabled only if all of the following are true 1 the ROTOR switch on the front panel is ON 2 the chamber cover is closed 3 any attached SC1021 controller permits rotation In Slave mode the user leaves the ROTOR switch ON and the SC1021 controls rotor rotation The front panel ROTOR switch serves as an emergency stop In Manual mode the front panel ROTOR switch controls rotation On the SC110A SC210A switching the unit ON also starts the sequence for evacuating the chamber see below Switching the SC110 OFF immediately begins braking the rotor Switching the SC110A SC210A OFF releases vacuum waits for a factory set interval and then begins braking In both cases the unit releases the brake when the rotor slows to 6 RPM Dynamic braking is achieved by applying DC voltage to the AC motor Chamber heater In both operating modes the DRYING RATE switch on the concentrator front panel controls the chamber heater Switching it to LOW disables the heater switching it to MED or HIGH enables the heater The MED and HIGH set
7. THE SC1021 SPEEDVAC SYSTEM CONTROLLER 55 sscsssnsoctietssavcasctssscedvasnecavesetine sossspeacaananbshess 8 40 SPECIFICATIONS scccccccssessccsscsccsenecsnsosvcnsecesssssovensesccesanscsscanecassneessons 8 5 0 ACCESSORIES AND ROTORS cccsccscssssecsssscsscressserensaccaseraneesssesscescaaor eas 9 5 1 e cio icelds Me EE 9 5 2 ROTORS FOR SPEEDVAC SC110 AND SPEEDVAC PLUS SC110A SC210A eee eere eere 10 5 3 ROTORS FOR SPEEDVAC PLUS SC210A ee 10 60 WARRANTY AND LIABILITY eene ee enenn nennt hne hne nnne nennen 11 APPENDIX 1 MAINTENANCE AND SERVICE e eese eee eee ettet e eee enhn nn nnne 12 APPENDIX 2 APPLICATION INFORMATION n een tnn enne nenne nennen 13 APPENDIX 3 TIPS TO ENHANCE PERFORMANCE eene ee nennen nennen nenne 14 SpeedVac is a registered trademark of Savant Instruments Inc SpeedVac concentrator is protected under U S Patent No 4 226 669 Pear shaped flasks are protected under U S Patent No D340993 Teflon is a registered trademark of E I duPont de Nemours amp Co 1994 Savant Instruments Inc 111 3001 REV B 3 94 DESCRIPTION The Savant SC110 SpeedVac and SC110A SC210A SpeedVac Plus centrifugal vacuum concentrators rapidly evaporate liquids to concentrate or dry solutes analytes and residues in aqueous and organic solvents Vacuum applied to the sample ch
8. and best results read this manual in its entirety before operating this instrument e Install the SpeedVac on a clean dry level stable surface within 4 ft 1 2 m of a compatible electrical outlet e Place the unit in a convenient location with access to a vacuum source Typically the SpeedVac sits on a countertop or on the mobile Savant CC120 DX Deluxe Convenience Cart Overhead clearance roughly equal to the unit s depth is required for raising the cover The SC110 SC110A concentrators may be placed on top of the optional SC1021 SpeedVac System Controller e Makesure theON OFF switch on the front of the SpeedVac is in OFF position Connect power cord to instrument and plug into appropriate outlet The safety lid locking mechanism on SC110 SC110A 240 V and SC210A 120 V and 240 V units will automatically disengage WARNING Before connecting the SpeedVac concentrator to an outlet make certain that voltage frequency and amperage match the requirements indicated on the label on the rear of the instrument SC110 SC110A 120 V AC 60 Hz 6A or 240 V AC 50 Hz 3 A SC210A 120 V AC 60 Hz 8 A or 240 V AC 50 Hz 4 A If there are any questions please consult an electrician Note To manually open the lid prior to applying power use the interlock override tool This tool may also be used to open the unit during a power outage Insert the tool at an upward angle approximately 45 into the small hole in the upper right
9. Reconfiguration The polypropylene vacuum fittings do not withstand strong lateral force Should you ever need to remove a vacuum line carefully pull straight out to slide the tubing off the fitting Reconfigure the SpeedVac and system components as desired To reattach the line place a thin film of vacuum grease on the fitting carefully slide new tubing straight on over fitting 13 111 3001 REV 8 3 94 APPENDIX 3 TIPS TO ENHANCE PERFORMANCE 1 TO INCREASE THE RATE OF SOLVENT EVAPORATION AND SHORTEN DRYING TIME TRY ONE OR MORE OF THE FOLLOWING Use glass tubes and vials in place of plastic Install and use a Radiant Cover on the SpeedVac Preheat the SpeedVac chamber and rotor using MEDIUM DRYING RATE Use rotors with metal tube carriers instead of plastic rotors e g RH12 20 RH8 18 These rotors conduct and transfer heat to tubes for faster sample drying Prewash plastic tubes with methanol to remove materials that inhibit or prevent evaporation of aqueous samples 2 TO KEEP VACUUM PUMP OIL CLEAN TRY THE FOLLOWING D Empty solvent s collected in the Refrigerated Vapor Trap as frequently as possible Change the oil in the vacuum pump when it becomes brown in color or smells of solvents being evaporated Attach a Savant VPOF100 Vacuum Pump Oil Filter Recirculator to your vacuum pump when evaporating acids e g HCl acetic TFA in the SpeedVac i e Attach a Savant SCT120 Chemical T
10. amber from an external source promotes solvent evaporation As the rotor in the concentrator spins centrifugal force keeps samples from foaming or bumping preventing sample loss Superior analyte recovery results Typical applications include concentrating or drying oligonucleotides ethanol precipitates of DNA hydrolysates of proteins chromatography fractions solid phase eluants liquid liquid extracts and samples for analysis by HPLC GC MS immunoassay and bioassay SpeedVac concentrators are also used in organic chernistry residue analysis such as pesticide and drug testing The SpeedVac SC110 model includes a built in manual bleeder valve on top of the unit to the right rear This instruction manual contains an explicit operating sequence see section 3 4 SPEEDVAC SC110 OPERATION that the user must follow to ensure vacuum is applied to the sample chamber only when the samples are spinning The SpeedVac Plus SC110A SC210A models include an automatic valve that ensures application of vacuum at the appropriate time without user interven tion All SpeedVac concentrators include a chamber heater to effectively counteract evaporative cooling and accelerate drying rate The operator can select low cham ber at ambient temperature medium chamber at 43 C or high chamber at 65 C For even shorter drying times an optional Radiant Cover further hastens drying rate by adding energy to the sample chamber SpeedVac co
11. ax 516 249 4639 for parts or repair For the SC110 SC110A order Savant part UMA100 For the SC210A order part UMA200 To determine if bearings are worn Open the sample chamber cover Unplug the instrument Remove the rotor hold down knob rotor and three screws Phillips on the chamber bottom mounting plate This plate of blue white plastic forms the top portion ofthe UMA which houses the bearings Replace the hold down knob Using the knob as a handle pull the drive shaft upward until the UMA comes free Pull carefully to release the magnetic attraction between the UMA and the Lower Magnet Assembly not visible located beneath the metal plate in the SpeedVac floor Hold the plastic mounting plate rigid and attempt to pivot the magnet bottom portion of the UMA around it If there is excessive play wobble between the plate and magnet replace with new UMA To replace the UMA Align holes then secure new UMA with the three screws Replace rotor Verify that rotor is seated properly on the aligning pins and that the hold down knob is hand tight Reconnect the instrument to an appropriate electrical outlet Resume operation Drive Shaft Upper Magnet Assembly Rotor Aligning Pins 12 111 9001 REV B APPENDIX 2 APPLICATION INFORMATION Dislocation due to braking When highly discrete solutes are dried using ordinary rotors and the total rotor mass is small some users note that the minute solute pellet is dislocated b
12. bottles RF8 100 Recovery flasks conical base 100 ml set of 8 RFA 8 Recovery flask adapters required for RF8 100 set of 8 cush ions and collars RH8 200 Rotor for 8 each 100 ml pear shaped flasks PSF100R Pear shaped flasks with receptacles 100 ml PSFA 200 Adapter for use of PSF100R flask in RH8 200 rotor AB250 Amber glass bottles 250 ml RH6 400 Rotor for 6 each 250 mi pear shaped flasks with receptacles PSF250R Pear shaped flasks with receptacles 250 ml PSFA 400 Adapter for use of PSF250R flask in RH6 400 rotor RH4 500 Rotor for 4 each 500 ml pear shaped flasks with receptacles PSFSOOR Pear shaped flask with receptacles 500 ml PSFA 500 Adapter for use of PSFSOOR flask in RH4 500 rotor MPR4 200 Microplate rotor includes 4 carriers MPTR8 210 Rotor for Marsh plates includes 4 carriers MPTR12 210 High capacity microplate rotor includes 4 carriers WARRANTY AND LIABILITY STATEMENTS AllSavant products excluding glassware are warranted against defects in material and workmanship for one year after the date of delivery to the original purchaser Savant s warranty is limited to defective materials and workmanship Warranty work is subject to our inspection of the unit No instruments equipment or accessories wil be accepted without a Return Material Authorization RMA number issued by Savant The warranty obliges you to follow the precautions in this manual When returning apparatus that may contain hazardous materials you must
13. d must be disconnected from the white cover by removing both the vacuum tubing and the electrical connector Alternatively you can unscrew the four recessed screws at the base of the concentrator to detach the vacuum solenoid from the blue casing o Take hold of the white cover and lift it straight up This is necessary because there are two guide pins beneath it To remove the chamber cover slide out the hinge pins using pliers or wire cutters Use a wood block or pencil to provide leverage and avoid damaging the paint finish 2 6 Upper Magnet Assembly The Upper Magnet Assembly accessible from the sample chamber contains bearings that can wear out since they are typically operated in a vacuum The Instruction Manual advises the user to lightly apply vacuum grease to the rotor shaft to extend the life of this assembly The Upper Magnet Assembly is a possible source of resistance to rotation and of noise Open the chamber cover Remove the hold down knob rotor and N three Phillips screws on the mounting plate Replace the hold down knob Pull the shaft upward until the magnet comes free You have removed the Upper Magnet Assembly Hold the magnet rigid and rotate the mounting plate If the rotation is difficult or uneven replace the assembly with a spare Upper Magnet Assembly For the SC110 or SC130A order Savant UMA100 For the SC210A order Savant UMA200 2 7 Lower Magnet Assembly If manual rotation of the Upper
14. e manual is designed to provide a step by step proc dure for generalists to evaluate a unit Later sections also give more applied information such as solutions to specific problem reports and detailed theory The manual does not explain how to repair individual components such as circuit boards Failed circuit boards can be returned to Savant where the Service Department uses customized testers to determine whether the board can be repaired and returned to service Other components should not be returned to Savant Organization All Savant service manuals are divided into these parts o Part 1 Theory of Operation describes the unit s purpose principles of design and sequence of operation Part 1 describes the diagnostic tools that are built into the unit o Part 2 Service Procedures shows how to determine whether a unit has failed how to identify and replace the faulty component and how to perform other required maintenance procedures o Part 3 Tables provides specifications for each model These specifications include acceptable measurements for each measurable parameter and specificacions and characteristics of the component subsystems and fluids Part 3 also lists order numbers for spare parts for each model o Technical drawings appear at the end of the manual Service notes updating this manual will be issued from time to time by Savant Service notes will reflect important changes in procedures or productio
15. ed flasks 50 ml with receptacles 8 each RH4 100 Rotor for 4 each 100 ml pear shaped flasks PSF100R Pear shaped flasks 100 ml with receptacles set of 4 PSFA 100 Adapters for use of PSF100R flasks in RH4 100 rotor RCSO Rotor for 50 each Beckman Ready Caps HRH4 Horizontal rotor head Holds up to 4 each swing out carriers always order 2 each of same carrier MCM22 Swing out carrier for 22 each 0 4 ml tubes MCRS Swing out carrier for 5 each 1 0 ml tubes MCM8 Swing out carrier for 8 each 1 5 ml tubes PREP100 Prep rotor for large volume evaporation includes package of 5 prep rotor liners Radiant Cover recommended PRL100 Prep rotor liners 5 3 ROTORS FOR SPEEDVAC PLUS SC210A RH200 12 Rotor for 200 each 12 x 75mm tubes or 118 each 13 x 100 mm tubes or 200 each 1 5 ml microcentrifuge tubes RH60 17 100Rotor for 60 each 17 x 100 mm tubes RH52 15 Rotorfor52 each 15m conical centrifuge tubes 17 x 120 mm RH48 18 125 Metal rotor for 48 each 16 x 125 mm tubes or 18 x 125 mm tubes includes carriers RH32 18 150Metal rotor for 32 each 18 x 150 mm tubes or shorter includes carriers 10 6 0 111 3001 REV B 3 94 RH50 28 60 Rotor for 50 each 28 x 60 mm scintillation vials RH12 29 Metal rotor for 12 each 28 x 150 mm tubes or 50 ml conical centrifuge tubes includes carriers RH26 50 Rotor for 26 each 50 ml conical centrifuge tubes 30 x 115 mm RH8 100 Metal rotor for 8 each 100 mi recovery flasks or 250 ml plastic centrifuge
16. hand panel of the SpeedVac concentrator While lifting up on the lid pivot the tool handle upward creating a downward movement within the SpeedVac This manually disengages the locking mechanism e Lift the lid and clean the chamber of any packing material or foreign items that may be present from shipping Lightly coatthe drive shaft and rotor center with vacuum grease This protects these surfaces from corrosive vapors Do not lubricate the cover O ring 0 111 3001 REV 8 3 94 Attach the concentrator to a vacuum system vapor trap plus vacuum pump Read this entire section before proceeding General directions Connect the 0 5 inch 1 27 cm O D hose fitting at the rear of the unit to the vacuum system Coat fitting with a thin film of vacuum grease Thread vacuum tubing through the hole in the instrument right side panel Without twisting carefully push vacuum tubing straight on over fitting Note Read the Reconfiguration section of this manual see APPENDIX 2 page 13 before disconnecting or replacing vacuum hose Protecting the vacuum pump A vacuum pump experiences loss of efficiency and ultimate damage if evaporated solvents from samples are allowed to enter To prevent damage and extend pump life a vapor trap that _condenses and preferably freezes solvent vapors must be placed in line between the SpeedVac concentrator and vacuum pump A complete line of vacuum pumps and refrigerated vapor tra
17. he value specified in Section 3 1 Test the resistance across the thermal fuse If the fuse is blown infinite resistance then replace it Any time you replace a blown thermal fuse look for an underlying problem Test the thermistor and ensure that it is connected to the circuit board Resistance across the thermistor should be about 10K ohms when the ambient temperature is 25 C Replace the thermistor if it appears to be bad Repairing or reattaching the thermistor will correct a runaway request for heat and will prevent blowing another thermal fuse CE After any repair concerning chamber heating check the operation of chamber heating after you replace the Heater Assembly Switch the ROTOR OFF place a thermometer in the chamber and test the MED and HIGH switch positions The chamber temperature should stabilize at 43 C or 60 C respectively If during this test the chamber ever exceeds 70 C and you have tested the thermistor replace the circuit board Return the old circuit board to Savant indicating a logic error in chamber heating 10 2 10 All Other Problems Problems with a single device Problems may occur with o the cover lock 220VAC units only o operation of the internal vacuum solenoid In these cases look at the corresponding LED on the circuit board the one labeled COVER for the cover lock or SAV for the vacuum solenoid to verify that the circuit board is trying to operate the device appropriately If no
18. ignated by the RH prefix followed by numbers denoting vial capacity and tube diameter For example the RH100 6 rotor accommodates 100 tubes of 6 mm diameter Also available are horizontal rotor heads HRH prefix for swing out carriers microplate rotors MPR MPTR prefix and a prep rotor PREP 100 for batch pro cessing CAUTION Only these rotor series should be used in the SpeedVac DO NOT use other rotors even if they seem to fit the instrument CAUTION Always balance rotor loads An unbalanced rotor causes vibration that will wear out the bearings and may seri ously damage the SpeedVac Load the rotor uniformly There need not be a tube in each place but the tubes must be evenly spaced around the rotor When using a rotor with aluminum tube holders insert tube holders symmetrically When drying different types of sample in the same drying run distribute each type symmetrically so that their different drying rates do not introduce preces sion into the rotor s rotation Always select a rotor into which the sample tubes fit snugly To install or change a rotor Verify that CONCENTRATOR switch is in OFF position Open the lid Unscrew the rotor hold down knob and remove the existing rotor if any Select desired replacement rotor Align the notches in the rotor base with the drive pin of the motor shaft Place the rotor on the shaft be sure the pins engage the notches Hand tighten the rotor hold down knob until firmly
19. ks The only required environmental check is that the unit be level Vibration and poor performance can be caused by operating the unit on a tilted surface If this is the case correct the environment and then retest the unit 2 3 Recommended Service Equipment o Volt ohm meter VOM e Variable frequency strobe light o Blade and Phillips screwdriver 2 4 Troubleshooting Aids There are five discrete LEDs on the circuit board that are numbered The function of these LEDs are as follows LED 1 Front panel LED always on if the DRYING RATE switch is HIGH If the radiant cover is installed LED 1 is ON only when the chamber heater and the radiant cover are both activated The LED is off whenever the rotor rotation is below 1000 RPM LED 2 Front panel LED always on if the DRYING RATE switch is MEDIUM LED 3 Always on if the DRYING RATE switch is LOW LED 4 Front panel LED always on if the ROTOR switch is ON LED 5 Always on if the ROTOR switch is OFF 5 There are also two banks of LEDs that provide additional status They are labeled on the circuit board The LEDs in bank LEDS1 have the following functions SLND On if the chamber cover solenoid is actuated to unlock the cover 220VAC units only CVR On if the chamber cover is closed SAV On if relay 1 is actuated if the unit has switched the internal SpeedVac Automatic Valve to apply vacuum to the concentrator chamber The LEDs in bank LEDS2 have the following fu
20. m RPM no load Delay before braking cycle starts ELECTRICAL Resistance of heater coil 115VAC units 220VAC units TEMPERATURES Medium chamber heating setting High chamber heating setting Thermal fuse cutout setting SC110 SC110A 12 9 32 8cm 11 3 28 7cm 17 6 44 7cm 36 lbs 16 kg 100 1675 RPM 1400 RPM 6 RPM 20 seconds 10 seconds SC110A only 50 ohms 200 ohms 43 C 60 C 98 C 12 SC210A 18 1 18 2 25 3 97 lbs 200 46 0cm 46 2cm 64 3cm 44 Xg 1675 RPM 1400 RPM 6 RPM 20 seconds 10 seconds 25 ohms 100 ohms 43 C 60 C 98 3 2 Spare Parts Order Numbers COVER HARDWARE Lucite cover with hook USA Lucite cover with hook Int l cover hook Cover hook nut Groove pin Cover switch assembly 115 VAC Switch Cover lock assembly 220 VAC Cover lock release tool SAMPLE CHAMBER COMPONENTS Chamber Chamber O ring seal Heater coil 115 VAC Heater coil 220 VAC Thermistor Thermal fuse 98 C Rubber foot MOTOR AND ROTOR COMPONENTS N Motor assembly 115 VAC Motor assembly 220 VAC Hold down knob Washer for hold down knob Upper magnet assembly Bearing plate for UMA Bearing for UMA Closure plate O ring for closure plate W CONTROL Circuit board 115 VAC Circuit board 220 VAC Fuse 4A Slo Blo Fuse holder VACUUM SpeedVac Automatic Valve 115 SpeedVac Automatic Valve 220 Vacuum fitting 110 SC110A 095 6012 00 095 6068 00
21. n Each service note will replace or add specific pages to this manual Insert these pages into the nanual as specified Discard any replaced pages Each service note will have as its first page a change history including it and all previous service notes Place the first page of the service note just after the cover page of this manual to show that the manual incorporates that and all previous service notes THEORY OF OPERATION 1 1 The Application The Savant SC110 SC110A SC210A concentrators spin one of a variety of rotors The rotors contain sample test tubes Vacuum can be applied to the sample chamber to withdraw vapors as the liquids evaporate Rotation of the tubes prevents bumping and foaming as vacuum is applied The units isolate the motor from the vacuum chamber The units include a two level electric heater Applying heat to the sample chamber encourages evaporation In addition the samples cool as some liquid evaporates applying heat counteracts this cooling and can protect the samples from freezing A rule that the Instruction Manual emphasizes is that vacuum should only be applied to the sample chamber when the rotor is spinning If this rule is not followed samples can bump foan and boil out of the tube The SC110A and SC210A SpeedVac Plus R concentrators have an internal bleeder solenoid the unit automatically controls this solenoid to comply with this rule The SC110 SpeedVac R concentrator does not have such a
22. ncentrators can be operated manually or attached to Savant s SC1021 SpeedVac System Controller unit for totally automatic operation The SC110 SC110A units hold up to 100 sample tubes the larger SC210A holds up 200 tubes 1 6 liters total volume A variety of available rotors accommodates microcentrifuge tubes test tubes microvials and mini and standard scintillation vials A line of large volume up to 500 ml multi sample rotors hold Savant s recovery flasks or pear shaped flasks with receptacle ends A prep rotor is available for concentrating large batch amounts of solution INSTALLATION Receiving Inspect the shipping carton upon receipt If the carton is damaged in any way do not accept delivery Call Savant at 1 800 634 8886 516 249 4600 or Fax 516 249 4639 111 3001 REV B 3 94 Unpacking Carefully remove the instrument and rotor from the shipping carton Compare the packing list to the box contents If there is a discrepancy call Savant at 1 800 634 8886 516 249 4600 or Fax 516 249 4639 Inspection Inspect the unit for any damage that may have occurred during shipment Should there be any damage report it to the carrier and contact Savant immediately Make sure the carrier inspects the damage and leaves an inspection report Register any claims for shipping damage against the carrier or his agent Save the shipping carton in the event a return is necessary Set up To assure safe operation
23. nctions HTR On if relay 2 is actuated if the chamber heater is on RCO On if relay 3 is actuated if the radiant cover is on BRK On if relay 4 is actuated if the unit is braking the rotor MOTOR On if relay 5 is actuated if the unit is rotating the rotor 2 5 Troubleshooting Sequence The presence of optional attachments affects the operation of the concentrator Therefore to simplify troubleshooting first remove any SC1021 controller connected to the rear of the concentrator Also disconnect any radiant cover from the rear of the concentrator Plug the unit into an operating electrical outlet of the proper voltage Quickly check the circuit board s voltage and logic by operating the DRYING RATE switch When there is no controller or radiant cover attached and when the chamber is at approximately ambient temperature the LEDs by the legends HIGH and MED should light up when you put the switch in the respective position In the LOW position neither LED should be lit o If neither LED is lit in any switch position look for a power fault Verify that the electrical outlet is operating by plugging a lamp or other appliance into it Unplug the unit remove the bottom plate and inspect the fuse F1 0 5 amp 250VAC on the circuit board 18 it is blown replace it and retest If the fuse is not blown plug the unit back in and carefully verify that AC is present at the power connector J8 If so return the circuit board If not re
24. o the concentrator there are only four locations where leaks or obstructions can occur Inspect them starting with the easiest to reach o Cover Open the cover and visually inspect the O ring for wear or degradation Replace it if necessary The cover O ring should never be greased o Rear vacuum fitting This fitting is rarely the source of the leak Inspect this fitting and replace it if necessary o Vacuum solenoid SC110A SC210A only To verify that the internal vacuum solenoid is not obstructing vacuum or leaking take it out of the circuit Connect the vacuum lines that lead to the vacuum solenoid to each other using a two ended tubing connector Then apply vacuum to the concentrator and see if adequate vacuun is reached If it is replace the solenoid o Bottom seal Remove the motor as described in Section 2 7 Remove the stainless steel closure plate exposing the hole in the sample chamber Visually inspect the O ring between these surfaces for wear or degradation Replace it if necessary Lightly apply vacuum grease to this O ring 2 9 Heater Assembly The heater is in the sample chamber casting Gain access to it by disconnecting power and removing the bottom plate The heater can burn out over time In addition the heater is protected by a thermal fuse that blows if the chamber temperature ever reaches 98 C Either event can impede chamber heating The heater needs replacement if its resistance is greater than t
25. on Cover Transparent 0 75 in 1 91 cm acrylic standard Safety interlock on SC110 SC110A 240 V models and SC210A 120 V and 240 V models CAUTION DO NOT apply heat to this cover serious deformation will occur under vacuum Optional Radiant Cover available for all units Optional Glass Safety Cover available for SC110 SC110A Vacuum Chamber Vacuum Fitting Controls Concentrator Drying rate Drive Rotors 111 3001 REV B 3 94 Chemical resistant impregnated aluminum casting with inert fluorocarbon coating Teflon 0 5 in 1 27 cm O D vacuum fitting Two position switch ON OFF Three position switch HIGH MEDIUM LOW 65 C 43 C Ambient Seal less magnetic coupling drive Over 40 available See section 5 0 ACCESSORIES AND ROTORS for selection Custom rotors available con tact Savant for details 1 800 634 8886 516 249 4600 or Fax 516 249 4639 5 0 ACCESSORIES AND ROTORS 5 1 ACCESSORIES RC110A RC210A GSC110 1021 SCT120 DC120A Radiant Cover for SC110A SpeedVac Plus Radiant Cover for SC210A SpeedVac Plus Glass Safety Cover for SC110 SC110A SpeedVac System Controller Chemical Trap Disposable Cartridge for SCT120 when trapping acid and water vapors DC120A 4 Disposable Cartridge for SCT120 when trapping acid and DC120R water vapors 4 pack Disposable Cartridge for SCT120 when trapping radioactivity and organic solvent vapors DC120R 4 Disposable Cart
26. pack and label them following U S Department of Transportation DOT regulations applying to transportation of hazardous materials Your shipping documents must also meet DOT regulations All returned units must be decontaminated and free of radioactivity Under no circumstances shall Savant be liable for damages due to the improper handling abuse or unauthorized repair of its products Savant assumes no liability express or implied for your use of this equipment 11 111 3001 REV B 3 94 APPENDIX 1 MAINTENANCE AND SERVICE Maintenance Maintaining a clean instrument is crucial for dependable results Clean spills immediately Dried solvents can build up impairing rotor rotation Periodic cleaning of the sample chamber prevents problems Use a detergent solution on a sponge or gauze then thoroughly wipe the chamber dry Excess moisture in the chamber is removed on the next SpeedVac run but can alter the performance of this run As described under section 2 0 INSTALLATION keep the drive shaft and rotor center lightly coated with vacuum grease Do not lubricate the cover O ring Upper Magnet Assembly Although the Upper Magnet Assembly UMA is equipped with sealed bearings using the SpeedVac with aggressive acids and bases can cause bearings to corrode over time Should this occur the UMA may need replacement frequency depends on individual usage patterns Call Savant s Service Department 1 800 634 8886 516 249 4600 or F
27. place the power cord or cable to the circuit board o Other abnormal patterns of LED lighting mean that you should return the circuit board to Savant Before checking the rotor operation ensure that a rotor is installed in the concentrator chamber and tightly held in place 7 using the rotor hold down knob Close the cover and switch the ROTOR switch ON Listen for excessive noise Allow 20 seconds for the SC110 SC110A to come up to speed allow 45 seconds for the SC210A to come up to speed Use the variable frequency strobe light to measure the rotation speed Shine the light into the rotor chamber and adjust the knob until the rotor appears to be stationary then read RPM off the knob If the unit is noisy when rotating or if the rotating speed is 20 less than its rated speed then service the motor as described in the next sections 2 55 Disassembly and Replacement Access to the interior of the concentrator requires that the blue casing be separated from the white cover Most of the internal devices are fastened to the white cover To separate the casing from the cover follow these steps o Use a Phillips screwdriver to remove the four screws from the base of the concentrator Do not remove the four recessed screws o At the rear of the white cover there are two bolts on the underside They are visible from the rear of the unit Use a 1 4 nut driver to remove these two nuts On the SC110A SC210A the vacuum solenoi
28. power on power off sequence 1 4 Overview of Maintenance Since the unit s behavior differs depending on the attached equipment it simplifies service to follow these rules o Always ask the customer which concentrator model is in use and in the case of the SC110A SC210A whether SC1021 controller or radiant cover is attached o Always detach any such apparatus before evaluating failed units If the customer reports a failure in Slave mode verify that the unit works in Manual mode then determine whether the unit sends correct signals to the connector SERVICE PROCEDURES 2 1 Safety Precautions The concentrator uses AC power and some of the service procedures in this chapter require operation with the cover off exposing power lines This raises the risk of electrical shocks The unit should only be plugged into a circuit protected by a Ground Fault Interruptor GFI This minimizes injury from shock In addition you should not touch exposed wires at all without first unplugging the unit An additional hazard to the equipment is as follows The circuit board and indicator panel contain electronics that can be damaged by static electricity by giving it a shock When removing a circuit board always hold it by the sides Persons doing extensive maintenance on circuit boards should be grounded such as by wearing wrist straps When shipping a circuit board always enclose it in a static protective bag 2 2 Environmental Chec
29. ps is available from Savant A chemical trap placed in line between the vapor trap and the vacuum pump further protects the pump from any vapors pulled through the trap Savant s SCT120 Chemical Trap may be used with the DC120A cartridge to adsorb aqueous vapors and neutralize corrosive acid vapors or with the DC120R cartridge to adsorb radioactivity and volatile organic vapors System configuration may vary depending on the needs preferences and application requirements of the user If uncertain of the optimal set up call Savant Technical Services at 1 800 634 8886 516 249 4600 or Fax 516 249 4639 for additional information OPERATION The SpeedVac or SpeedVac Plus concentrator is an important component in a sample drying concentration system that should also include vacuum gauge refrigerated vapor trap chemical trap and vacuum pump and may contain other equipment Please refer to operating instructions of each component for details on use 3 1 CONTROL PANEL Two switches on the instrument front panel govern operation of the SpeedVac SC110 SC110A SC210A concentrators The CONCENTRATOR switch controls rotation ON OFF The DRYING RATE switch regulates sample chamber temperature HIGH MEDIUM LOW Indicator lights illuminate when a function is switched on 111 3001 REV B 3 94 3 2 ROTORS A wide variety of SpeedVac rotors is available to suit every sample drying application These rotors are des
30. rap with acid neutralization cartridge when evaporating acids i e HCl acetic TFA in the SpeedVac If you are using a gel dryer with your SpeedVac remove the gel dryer from the system Use an oil free maintenance free Savant Gel Pump GP110 for the gel dryer SAVANT SERVICE MANUAL 01 Apr 92 SC110 SpeedVac Concentrators 10 and SC210A SpeedVac Plus R Concentrators CONTENTS ee Preface THEORY OF OPERATION The Application Theory of Operation Sequence of Operation Overview of Maintenance PUNE SERVICE PROCEDURES N ubleshooting Safety Precautions Environmental Checks Recommended Service Equipment Troubleshooting Aids Troubleshooting Sequence NNN NN H 3 Lei ae TUE ailed Procedures a Disassembly and Replacement Upper Magnet Assembly Lower Magnet Assembly Vacuum Problems Heater Assembly 11 Other Problems e Houat UPRWNHO NNNMNNN O amp e SPARE PARTS LIST Specifications Spare Parts Order Numbers WW CJ NP 4 ENGINEERING DRAWINGS Preface This manual explains how to detect diagnose and correct malfunctions to the component level in the Savant SC110 SpeedVac R and the SC110A and SC210A SpeedVac Plus R concentrators It is written for Savant manufacturing personnel and for Savant field service personnel It is also available to trained customers who wish to perform their own maintenance Th
31. ridge for SCT120 when trapping radioactivity and organic solvent vapors 4 pack 111 3001 REV B 3 94 5 2 ROTORS FOR SPEEDVAC SC110 AND SPEEDVAC PLUS SC110A SC210A RH40 6 Rotor for 40 each 6 x 50 mm tubes includes carriers RH100 6 Rotor for 100 each 6 x 50 mm tubes RH100 8 Rotor for 100 each 8 x 29 mm tubes RH40 11 Rotor for 40 each 1 5 ml microcentrifuge tubes includes adapters RH64 11 Rotor for 64 each 1 5 ml microcentrifuge tubes _ RH120 11 Rotor for 120 each 1 5 ml microtubes RH20 12 Rotor for 20 each 12 x 75 mm or 10 each 13 x 100 mm tubes includes carriers RH40 12 Rotor for 40 each 12 x 75 mm tubes RH60 12 40 Rotor for 60 each 12 x 32 mm or 12 x 40 mm vials RH72 12 Rotor for 72 each 12 x 75 mm tubes RH32 13 Rotor for 32 each 13 x 100 mm tubes RH24 15 Rotor for 24 each Waters WISP autosampler vials RH8 17 5 Rotor for 8 each 15 ml Corning Corex 17 5 x 102 mm tubes RH8 18 Rotor for 8 each 17 x 100 mm tubes includes carriers RH10 15 Rotor for 10 each 15 ml conical centrifuge tubes 17 x 120 mm RH24 18 Rotor for 24 each 18 x 52 mm mini scintillation vials RH12 20 Rotor for 12 each 20 x 60 mm reaction vials or 20 x 47 mm vials RH6 25 Rotor for 6 each 30 ml Corning Corex 25 x 105 mm tubes RH12 28 Rotor for 12 each 28 x 60 mm scintillation vials RH4 18 150 Rotor for 4 each 18 x 150 mm tubes RH6 50 Rotor for 6 each 50 ml conical centrifuge tubes 30 x 115 mm RH8 50 Rotor for 8 each 50 ml pear shaped flasks PSF50R Pear shap
32. seated 3 3 PREHEATING Evaporative sample cooling that occurs under vacuum actually decreases sample drying rate The sample chamber heater counteracts this effect to accelerate drying rate If desired operate the DRYING RATE switch at least 15 minutes before starting the concentration run LOW leaves the chamber at ambient temperature i e chamber heater is off MEDIUM heats the chamber to 43 C HIGH heats the chamber to 65 C 111 3001 REV 8 3 94 3 4 SPEEDVAC SC110 OPERATION Load samples symmetrically Verify that the rotor is firmly seated and the hold down knob is hand tight Close the cover and set the CONCENTRA TOR switch to ON Allow rotor to reach full speed approximately 20 seconds before manually applying vacuum with the bleeder valve This valve exposes spinning samples in the sample chamber to vacuum from the vacuum system and at the end of the min bleeds the chamber back to atmospheric pressure Premature application of vacuum can cause bump ing and foaming in some cases sample could boil out of the tubes The blue bleeder valve is located on the top right toward the rear of the instrument Turn arrowhead indicator toward concentrator OPEN to apply vacuum If necessary select DRYING RATE as described in section 3 3 Continue the run until a predetermined time has elapsed or until a vacuum gauge configured in line between the SpeedVac and vapor trap indicates a predetermined
33. t replace the circuit board Return the old circuit board to Savant indicating a logic failure in the operation of that device Analyzing the relay contacts or circuit board connectors may reveal a bad relay Handle this problem by replacing the circuit board If the signals are good at the connectors then repair or replace the affected device C1021 problems If problems occur only when the SC1021 is attached use the VOM or oscilloscope to analyze the signals on the 9 pin connector to ensure they are correct Pins enabling remote control of the concentrator Pin 1 Grounded by SC1021 controller if it is present Pin 2 GROUND reference for pins 1 6 produced by concentrator Pin 3 Grounded by SC1021 controller to start a concentrator run switched to high impedance by controller to start the braking sequence Pin 4 Grounded by the concentrator if it is present Pin 5 Grounded by the concentrator when the cover is closed Pin 6 Grounded by the concentrator when the rotor reaches 67 of its maximum RPM Pins enabling remote operation of the vacuum solenoid Pin 7 Return neutral Pin 8 Switched AC power to the solenoid Pin 9 GROUND 11 y SECTION 3 3 1 Specifications PHYSICAL Height Width Depth Weight Maximum capacity of sample tubes with standard Savant rotor Maximum shaft RPM no rotor Typical figure for 60Hz units Typical figure for 50Hz units End of run detection setting TIMING Typical time to maximu
34. tings enable circuits factory set at 43 C or 60 C respectively The circuit board switches off the chamber heater when the chamber achieves the respective temperature When a radiant cover is attached to the SC110A SC210A the effect of the HIGH setting changes In the HIGH setting neither the radiant cover nor the chamber heater will operate until the chamber rotation reaches 1000 RPM Rotation sensor A Hall effect switch senses rotation of the rotor The SC110A SC210A provide an up to speed signal based on this switch at the 9 pin connector in case an SC1021 controller is connected On SC110s and SC110As configured for 220VAC and all SC210As the unit unlocks the cover only when this switch detects that rotor speed is less than 6 RPM The cover is normally locked it is locked when the power is off Whether or not there is a cover lock a switch senses whether the cover is closed This signal goes to the 9 pin connector in case an SC1021 controller is connected Vacuum solenoid The controller uses three of the 9 pin connectors to operate the vacuum solenoid inside the SC110A SC210A The controller switches the solenoid tp vacuum only when rotor rotation reaches 1000 RPM The concentrator does not include a vacuum gauge The SC1021 controller or an external vacuum gauge such as the Savant VG5 is used to read vapor pressure in the vacuum line going to the concentrator chamber The concentrator does not have any special
35. vapor pressure in the sample chamber To devise a standardized endpoint time or vapor pressure for a specific application when repeatability of results is important conduct one or more test runs with expendable batches of sample At the end of the run turn arrowhead indicator on bleeder valve toward the left CLOSED to bleed the sample chamber to atmosphere allow 10 seconds for pressurization Stop the run by setting the CONCENTRATOR switch to the OFF position The rotor brakes until rotation decreases to 6 RPM Do not open the cover until rotation has completely stopped All 240 V units have a cover lock that prevents cover release at rotor speeds gt 6 RPM To shut off chamber heater set DRYING RATE switch to LOW 3 5 SPEEDVAC PLUS SC110A SC210A OPERATION Load samples symmetrically Verify that the rotor is firmly seated and the hold down knob is hand tight Close the cover and set the CONCENTRA TOR switch to ON Bleeder valve operation in SpeedVac Plus models is automatic Aspeed sensor inside the concentrator applies vacuum when the rotor reaches 800 RPM If necessary select DRYING RATE as described in section 3 3 When the mun is stopped by setting the CONCENTRATOR switch to the OFF position the unit immediately bleeds the rotor chamber then waits 10 seconds before braking the rotor Do not open the cover until rotation has completely stopped Shut off chamber heater by setting DRYING RATE switch to LOW 4 0 111
36. y the concentrator s braking action impeding recovery For these critical applications Savant recommends the use of a heavier rotor such as the RH64 11 or the HRH4 rotor head which holds two or four swing out buckets The conical tip of the sample tube swings out horizontally as rotation increases This draws solute to the tip of the tube minimizing pellet dislocation on braking Typical results The following data were gathered using Savant s SC110 SpeedVac concentrator RVT100 55 C Refrigerated Vapor Trap VP100 113 liter min vacuum pump the RH20 12 rotor and 20 glass tubes of 12 x 75 mm each containing 2 ml of solvent Time is shown as hours minutes These approximate drying times are presented as a guide only Results will differ depending on type of vacuum pump temperature and capacity of vapor trap integrity of seal between fittings and connections degree of contamination number of tubes per run and other factors Further improvements in evaporation rate Rotors with aluminum tube carriers such as the RH20 12 conduct more heat to the samples and enhance evaporation To speed evaporation further use a Savant Radiant Cover For the SC110 SC110A order the Savant RC100 Radiant Cover For the SC210A order the Savant RC200 Radiant Cover WARNING Never shine a lamp or heat lamp through the standard acrylic chamber cover The cover absorbs heat and wil deform especially while the sample chamber is under vacuum
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