Home

F44 How to use Leica TP1020 Automatic Tissue Processor

1. Fig 17 delivered with two wax baths no 11 and 12 Optionally a third wax bath can be connected to station no 10 4 6 1 Installing the wax baths i The Leica TP1020 basic instrument is e Insertthewaxbath connection cable 1 in the notch 2 in the platform and mount the wax bath onto the station holder 3 Leica TP1020 33 4 instrument Setup 4 6 2 Connecting the wax baths The connection plugs of each wax bath has to he inserted into the 1 corresponding socket number at the rear of the instrument e Rotate the instrument so that the sockets are easily accessible e Insert plug number 12 into socket number 12 _ 12 e Insert plug number 11 into socket number 11 ll e Lock clip 1 at each a 10 plug ES a Fig 18 Before connecting a third wax bath to station no 10 the cover cap has to be removed Finally the standard instrument configura tion has to be altered accordingly For detailed instructions see Chapter 15 Modifying the instrument configuration 34 Instructions for Use V 2 7 Rev A English 04 2011 4 Instrument Setup 4 6 3 Fastening the wax bath connection cables instruments with fume control system only Instruments with fume control system are equipped with a Plexi glas fume containment shield which consists of two parts To en sure the fume containme
2. Container station holder with level indicator Notch for wax bath connection cable Container station platform Control panel Fig 2 18 Instructions for Use V 2 7 Rev A English 04 2011 3 Instrument Components and Specifications General overview Leica TP1020 with additional wax bath optional accessory continued Continued As ba Voltage selector Mains switch ae Socket for mains cable 12 11 10 EURI Sockets for wax bath connector plugs socket of station no 10 with a cover cap not shown closed Wax bath connector plug Leica TP1020 Fig 3 TP1020 rear side 19 3 Instrument Components and Specifications 3 5 General overview Leica TP1020 with fume control system Plexiglas fume containment shield Fig 4 20 Instructions for Use V 2 7 Rev A English 04 2011 3 Instrument Components and Specifications General overview Leica TP1020 with fume control system continued Activated carbon filter receptacle Hose clamp Fume outlet tube le optional accessory
3. D U pose oo H ooo gogg CE Instructions for Use Leica TP1020 V2 7 RevA English 04 2011 Order No 14 0422 80101 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica TP1020 Automatic Tissue Processor Seica MICROSYSTEMS NOTE The information numerical data notes and value judgments contained in this manual represent the current state of scientific knowledge and state of the art technology as we understand it follow ing thorough investigation in this field We are under no obligation to update the present manual periodically and on an ongoing basis ac cording to the latest technical developments nor to provide our customers with additional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we ex clude liability as far as permissible according to the national legal system applicable in each indi vidual case In particular no liability whatsoever is accepted for any financial loss or consequen tial damage caused by or related to compliance with statements or other information in this manual Statements drawings illustrations and other information as regards contents or technical details of the present Instructions for Use are not to be considered as warranted characteristics of our products Published by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19
4. 11 12 may still be solid when basket 1 is reached When these warning codes are displayed a short sound signal very short sound very short sound is emitted and repeated in 5 minute intervals W 01 W 03 are displayed by the processing cycle upon occurrence e g when the basket is due to be immersed into a wax bath as programmed W 04 W 06 are displayed immediately when starting an automatic processing cycle of less than 8 hours duration from start until the basket reaches the first wax bath To quit warning codes W 01 W 03 press any key To lower or lift the tissue basket press KEY and ARROW UP or ARROW DOWN simultaneously 67 10 Warning and Error Codes Troubleshooting Warning codes continued il W 01 W 03 block the transport of the tissue basket to the wax bath no 10 11 or 12 If you know though that the paraffin is actually liquefied you can immerse the tissue basket into or remove from one of the wax baths manually H To override the warning codes W 04 W 05 and W 06 press KEY and START simultaneously to start processing Code Possible root cause Troubleshooting Warning code Paraffin in wax bath stations 10 11 12 To quit the warning code press any W 01 W 02 W 03 still solid key in manual Check if the paraffin is really molten processing mode If you find that the paraffin is molten pause the automatic processing cy cle and in the manual processing
5. DO NOT continue to use the wax bath Check if the wax bath works trouble free Use of defective wax baths has to be discontin ued for safety reasons e Empty the wax baths completely before the paraffin cools When cooling down the paraffin contracts which can cause damage to the wax bath Activated carbon filter optional accessory e Warning Fire hazard If a filter becomes saturated with solvent there is a potential fire risk It is important that the activated carbon filters are changed at factory recommended intervals Leica TP1020 11 3 Instrument Components and Specifications 3 1 Technical data Type TP1020 Approvals EE UL cUL VDE Electrical data Nominal voltage c c cccccssessesssssesecsessessesssssssssesssssesssssssussessssssessssssnssesseeseeseeneass 100 120 230 240 V AC 10 Nominal frequency E 50 60 Hz Power fuses MDA by Bussmann 2x T 10 0 A L250V UL listed Nominal POWE E 700 VA Classifications according to IEC 1010 EN61010 1 Protecive sclass ssrsaciontafa E EE E E EE EE EEN l Pollitionid egreer al 2 Overvoltage calegory acarasanana aa nana Anania RA LARA NARA RELA Lana Anna RA SERRAR R NNR Il Maximum heat EE 700J s Operating temperature range ciccia iaia iaia rara 5 C 40 C Relative NUMIdity ENEE 80 non condensing Dimensions Earpusglenwgt ege eege Eeer den 820 mm H ELE 595 780 mm Pitch diameter Of rollers EE 610mm H Weight Net weight inc
6. Fig 5 Leica TP1020 21 3 Instrument Components and Specifications 3 6 Accessories supplied 3 6 1 Standard tissue basket Fig 6 3 6 2 Glass beaker with holder 22 Capacity 100 cassettes Tissue cassettes are loosely arranged inthe bas ket The maximum capacity is 100 cassettes The cassettes are stacked in the basket So that the infiltration process is not disturbed no cassette may lie on top of the stack The maximum filling level must be reduced Depending on the size of the specimens and the use of foam supports or paper towels the minimum filling level can also be used as the maximum filling level For process ing 100 cassettes the maximum filling level forthe reagents is approximately 1200 ml Holding capacity 1 81 The reagent container consists of a glass beaker with holder The wall ofthe beaker has two marks Min Max that specify the minimum and maximum level in the container The level must not exceed the Max mark or fall below the Min mark during filling Instructions for Use V 2 7 RevA English 04 2011 3 Instrument Components and Specifications 3 6 3 Aluminum container with holder standard outfit in instruments with vacuum function or available as optional accessory for other types Holding capacity 1 81 Instruments equipped with vacuum function are supplied
7. and if used 10 are heated temperature controlled wax baths that can be filled with either wax pellets or molten paraffin wax Embedding cassettes used to hold the tissue samples are placed into the tissue basket The basic instrument is designed for a single tissue basket An optional second basket can be added The basket or baskets are moved clockwise from station to station To ensure thorough infiltration the basket containing the tissue samples is agitated by moving up and down at each station This function can be switched off at any time During processing as the tissue basket moves from station to station there is a delay period of sixty 60 seconds during which time the basket is sus pended above the station Excess liquid can drip down during this process This ensures that there is minimal reagent carryover from station to station All instrument functions are activated through the control panel Real time is displayed via LCD The instrument can be operated in manual and automatic processing mode Automatic processing is controlled via 9 different programs which can be individually set up altered and edited If a power failure occurs the specimens are protected from drying out even when overnight processing has been selected since in case of a pow er failure the tissue basket will always be immersed into a station Once mains power is restored processing will be resumed where it had been in terrupted After a long
8. the cursor jumps to the next data entry position in the direction of the arrow e To switch lines press ARROW LEFT PLUS MINUS buttons To modify the parameter in the position where the cursor is located e To increase the numerical value press PLUS e To decrease the numerical value press MINUS e To change the number of baskets from 1 to 2 press PLUS e To change the number of baskets from 2 to 1 press MINUS e To change the station number press PLUS or MINUS respectively e To switch onthe vacuum function types 2 and 4 push PLUS e To deactivate the vacuum function types 2 and 4 press MINUS 41 6 Programming the Instrument 6 1 General description Station CO JO OO SWAN a Jee N oO CO Reagent Formalin Formalin Alcohol 70 Alcohol 80 Alcohol 96 Alcohol 100 Alcohol 100 Alcohol 100 Xylene Xylene Paraffin Paraffin VAC lt lt Z lt ZZZZZZZZZ lt Example of a one basket program Duration 1h00 1h00 1h30 1h30 1h30 1h00 1h00 1h00 1h30 1h30 2h00 2h00 42 Programs are created step by step in the pro gramming mode Programming parameters have to be entered for each station individually Forthat purpose the cursor is moved to the correspond ing data entry position with the ARROW buttons The actual parameters are entered with the PLUS MINUS keys All entered parameters are memo rized immediately The TP1020 has a memory capacity of
9. 1 h xie P4 16 30 i H Lloi vac 1n00 sip GE END 2 09 48 Leica TP1020 e Press PLUS MINUS to enter the number of days e Press ARROW to move the cursor to the data entry position for the hours e Press PLUS MINUS to enter the number of hours e To move the cursor to the digits destined for entering the minutes push the ARROW key e Press PLUS MINUS to enter the number of minutes The program shown here would be started the next day at 7 30 PM To activate the delay function press START Once the delay function has been activated the tissue basket will move immediately to the select ed starting container While the basket is waiting in the starting con tainer for the actual program to start the display reads h When pressing CLOCK the display switches from realtime indication to reading the selected start ing time and the resulting end time Display end of processing time To check if the programmed starting time leads to an acceptable end of run time e Press CLOCK In the example shown here the end of process ing would be the day after next at 9 48 AM 59 9 Operating the Instrument in the Automatic Processing Mode Delayed start continued Edit and change starting time when using the delayed starting function e Press CLOCK again e Check the selected starting time and if neces sary change it as appropriate to achieve the desired end or run time e To act
10. 1h00 3 V 1h00 4 V 1h00 5 V 1h00 6 V 1h00 7 V 1h00 8 V 1h00 9 V 1h00 10 V 1h00 11 Paraffin V 1h00 12 Paraffin V 1h00 94 Instructions for Use V 2 7 Rev A English 04 2011 15 Appendix Leica TP1020 Programming Worksheet Program No Name Date Written by Station Reagent VAC Duration Comment S o nl olo B iw lye Paraffin N Paraffin Leica TP1020 95 15 Appendix Leica TP1020 Programming Worksheet Program No Name Date Station Reagent VAC Duration Comment Iesel IEN III lw lnm ed Paraffin N Paraffin 96 Instructions for Use V 2 7 Rev A English 04 2011 16 Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in house testing standards and that the product is fault less and complies with all technical specifications and or agreed character istics warranted The scope of the warranty is based on the content of the concluded agree ment The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Technical service information If you require technic
11. 9 programs Each program can be set up and edited by the user Programs nos 6 to 9 have already been set up ex works Programs nos 6 and 7 contain a short term or long term program for one tissue basket each Programs nos 8 and 9 contain a short term or long term program for two tissue baskets each These programs can be edited Instructions for Use V 2 7 RevA English 04 2011 6 Programming the Instrument 6 1 1 Special characteristics of two basket programs Station ornoo RUN e Jet ee N O CD Reagent Formalin Formalin Alcohol 70 Alcohol 80 Alcohol 96 Alcohol 100 Alcohol 100 Xylene Xylene Paraffin Paraffin Paraffin Example of a two basket program VAC Duration 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 Leica TP1020 In one basket programs for every processing sta tion a different infiltration time can be selected However in two basket programs infiltration time is the same for every processing station Basket no 1 starts in station no 2 and basket no 2 starts to be processed in station no 1 If a one basket program is modified into a two basket program by adding a basket the instru ment automatically selects station no 2 as start ing container At the same time the infiltration time for all stations is selected the same as the infil tration time for the actually displayed station These changes alter the previous one bas
12. a processing cycle is iM Progress sc ssesseseeseceeeeeeeeeeee 62 9 3 Displaying end of processing time 94 Pa singa Processione iaia il 9 5 ResUmMINga paUSEd process 9 6 Stopping or terminating a process 9 7 End of an automatic process 9 8 Removing the specimens s 9 9 Finishing the dally routine E Warning and Error Codes Troubleshooting scssssssssssssssessessesessseeesseneesesneatsseeneenseneeseass 67 VON Waning Code Siaran reiege 67 10 2 Error message Sines ee aana aa a e arita a ino 69 10 3 1 POWER FAILURE and WRONG STATION Messages 71 10 3 2 Measures to remove specimen material in case of prolonged power failure 72 10 4 ABORT Message iii CEL EE Maintenance 12 1 General maintenance instructions ececsesseecsecsesssecsecsecsesseeesecsuesessucsuecssesueesecsucseesussueeseesneeseesnesees RTE un WEE 12 2 1 Altering the standard working temperature iii 12 2 2 Reset after excess temperature shutdown 12 3 lte Leica TP1020 5 Table of contents 13 14 15 Optional AGGESSONESs Lauaccieieiaaa 13 1 Three level tissue basket 13 2 Basket removal device 13 3 Basket holder for second tissue basket 134 Connecting the fume outlet tube optional accessory for instruments With fume control SYSTEM iii i 86 13 5 Inserting the activated carbon filters o
13. are dishwasher proof e Take the glass or aluminum containers out of the beaker carriers and wash them in the dishwasher e Fill the wax baths and reagent containers and put them back into place e Reconnect the wax bath plugs e Switch on the mains switch Cleaning the Plexiglas fume containment shields of instruments with fume control system e Use a soft plastic spatula for removing residual paraffin from the surface of the shields to avoid scratches e Apply some alcohol or xylene on a cloth and wipe off the surfaces previously cleaned with the plastic spatula Do not allow xylene or alcohol to react on the surfaces 76 Instructions for Use V 2 7 Rev A English 04 2011 12 Maintenance 12 1 General maintenance instructions Leica TP1020 A For purposes of maintenance or repair the instrument may only be opened by service technicians authorized by Leica Overall the instrument can be considered maintenance free To ensure its trouble free functioning over a prolonged period of time we still recom mend Have a preventive maintenance done once a year by a service engineer authorized by Leica Once your warranty period expires we recommend to purchase a Leica Service Contract For details please contact your local Leica Service Organization Mop up spilled reagents immediately Clean the instrument on a daily basis see Chapter 11 Cleaning Once a month lift the carousel cove
14. container e Caution In case of a power failure the carousel automatically descends into a station e Instruments equipped with vacuum function may only be operated with the aluminum containers supplied together with the instrument Glass beakers used in combination with the vacuum accessories have a high hazard potential when damaged e While operating the instrument no liquid may enter in contact with any of the electrical connections or the interior of the instrument e Make sure to observe the level indicators on the reagent containers and wax baths Warning Use caution when handling solvents Make sure the premises are adequately ventilated Explosion hazard e Spilled reagents have to be wiped away immediately In case of long term exposure the instrument surfaces are only conditionally resistant to solvents Leica TP1020 9 2 Safety Operating the instrument continued e Always observe worker s protection rules and use adequate protective gear gloves laboratory coats The heated wax baths may only be used with paraffin Under no circumstances may they be filled with solvents When solvents heat a highly explosive mixture builds up Caution The interior containers of the wax baths become very hot when the heating func tion is activated Do not touch the gray upper rim of the containers with your hands Risk of injury Caution when handling hot paraffin Risk of injury Cleaning Bef
15. in progress can be displayed but not changed 1 Programs can be edited and changed while a processing cycle is e Activate the programming mode e Select a program To verify and or change the selected parameters e Move the cursor to the station number entry position ARROW RIGHT LEFT cursor buttons e Modify the station number pressing PLUS or MINUS and thus edit and if necessary alter step by step all station parameters AII modifications are saved immediately The program currently in 1 progress cannot be changed e Press PROG to quit programming 9 3 Displaying end of processing time e While a processing cycle is in progress it is possible to display the expected end of run time e To display end of run time press CLOCK 62 Instructions for Use V 2 7 Rev A English 04 2011 9 Operating the Instrument in the Automatic Processing Mode Display end of processing time continued In the example shown here end of processing would be the next day at 10 38 AM ln 0h50 Zeck PZ END 1 10 38 e To quit the display indication press CLOCK e again Automatic specimen processing can be interrupt ed and resumed later for example to reload spec 9 4 Pausing a process imens PAUSER e To interrupt the program press the PAUSE CONT button LJo1 vac 0h12 P4 PAUSE PAUSE is displayed While a run is paused PAUSE will be displaye
16. is activated Do not touch the gray upper rim of the con tainers with your hands Risk of injury The inside of the interior container 4 is coated with Teflon The interior container gets very hot It has minimum and maximum level indicator marks 5 H the excess temperature switch off mechanism reacts several times call Technical Service without delay DO NOT continue to use the wax bath Empty the wax baths completely be fore the paraffin cools When cooling down the paraffin contracts which can cause damage to the wax bath Instructions for Use V 2 7 RevA English 04 2011 4 Instrument Setup 4 1 Unpacking instructions Fig 10 ately When the instrument arrives check the tilt indicator Fig 10 on the package If the arrowhead is blue the shipment was transported laying flat was tilted at too great an angle or fell over during transport Note this on the shipping documents and check the shipment for possible dam age If obviously there is damage please contact your freight forwarder immedi Leica TP1020 A Caution when removing the metal strips There is a risk of injury when opening them the strip has sharp edges and is un der tension e To remove the metal strips 1 you need metal shears and suitable gloves Stand next to the crate and cut the strips at the location shown see Fig 10a lt above e Liftthe li
17. mode by pressing KEY and the cor responding ARROW button simulta neously lower the basket into the wax bath or lift it out of the wax bath Warning code WOW W 05 W 06 when starting an automatic processing cycle 68 Total duration of program from start until the basket reaches the first wax bath is less than 8 hours Paraffin in sta arrival of tissue basket tions 10 11 12 possibly still solid upon Check if there is sufficient time for the paraffin to liquefy completely until the basket arrives at the wax bath If this is not ensured fill the wax bath with molten paraffin To override quit the warning code press KEY and START simultaneously Instructions for Use V 2 7 RevA English 04 2011 10 Warning and Error Codes Troubleshooting 10 2 Error messages E 1 3 LJo 4 i On the next page you will find a com plete list of all error messages their meaning and how to troubleshoot them List of error codes To indicate a series of malfunctions the error codes E 01 to E 13 are displayed Three consecutive and very short sound signals that are repeated every 10 seconds reinforce the visual error code To quit the error codes press any key Code Possible root cause Troubleshooting Error code E 01 Cache memory defective Call Technical Service Error code E 03 E 04 E 05 Drive malfunction Switch off t
18. reading All key functions with the exception of the locking key itself are now locked Non of the key functions can be activated When pressing any key the dis play will always read LOCKED for 10 seconds e To unlock the key functions press KEN again for 5 seconds All key functions will return to normal CLOCK symbol button To setthe time and to indicate the total duration start time and expected end of automatic tissue processing programs The display differentiates between indication of time real time start time when working with de layed starting function end time which is indi cated through hyphens and colon and indica tion of duration holding time of the tissue basket in a particular station total duration of process which is indicated through the letters d and h 37 5 The User Interface THREE ARROW button for carousel up and down movement 38 The THREE ARROW button is used to start and stop the carousel tissue basket up and down movement When this function is activated the tissue basket is lifted and lowered in three second intervals within the processing stations This ensures a uni form mixing of all liquids and an optimized tissue infiltration This function is automatically activated when an automatic processing cycle is started It can be switched off and back on at any time also when no processing cycle is running With the function being activat
19. term power failure critical excess immersion time in a station will be visually displayed Instructions for Use V 2 7 Rev A English 04 2011 3 Instrument Components and Specifications 3 3 Standard delivery The Leica TP1020 is available in eleven different configurations which are described below All variants also include accessories as described in the table on page 17 D If the vacuum function or extraction is required for the intended processes to be carried out using this instrument this information must be included in the original order It is not possible to retrofit any instrument variant for use with vacuum function or extraction Type 1 One basket operation standard tissue basket Without vacuum without fume control 1 Basic instrument 12 Glass beakers with handle 2 Wax baths 1 8 liter 1 Standard tissue basket Type 2 Two basket operation double loading standard tissue baskets Without vacuum without fume control 1 Basic instrument 11 Glass beakers with handle 3 Wax baths 1 8 liter 2 Standard tissue baskets Type 3 One basket operation standard tissue basket With vacuum without fume control 1 Basic instrument 10 Aluminum reagent containers for vacuum 10 Reagent container carriers 2 Wax baths 1 8 liter 1 Standard tissue basket Type 4 One basket operation standard tissue basket Without vacuum with fume control system 1 Basic instrument 12 Glass beakers with handl
20. that is resistant to chloroform When working with paraffin that has a melting point below 58 C the in strument working temperature can be readjusted with the corre sponding setting screw e Use a screw driver to turn the setting screw A Seica 1 to the desired value If you find that the paraffin does not melt com WAX BATH TP 1020 pletely after lowering the working temperature ee 0422 30665 slightly readjust again i Factory set standard working temperature is 65 C 70 C on the 550 55 60 65 oc 1 Leica Microsystems Nussloch GmbH Madein D 69226 Nussloch Germany Fig 21 Leica TP1020 49 7 Preparations for Tissue Processing Altering the standard working temperature continued 50 Do not overfill the wax baths The level of the liquid paraffin must not exceed the upper fill level mark on the inside of the wax bath e To fill the wax baths use wax pellets or paraffin which has already been liquefied When filling the station make sure the paraffin level is not below the minimum level in which case there is a risk that not all speci mens will be entirely immersed in paraffin and thus will not be in filtrated completely It may take several hours to liquefy solid paraffin Make sure to calculate the waiting time When refilling wax pellets again make sure to observe the waiting time for complete liquefaction e Place the wax bath onto the correspo
21. the instrument Leica TP1020 29 4 instrument Setup 4 3 2 Adjusting the voltage selector Fig 14 30 When adjusting the voltage selector setting the instrument must be disconnected from the mains Insertthe small screw driver 4 into the small er one of the two notches at the lower end of the lock 5 using the screw driver as a lever Remove the shell 3 Remove the voltage selector 6 from the shell 3 and reinsert it so that the correct voltage selection can be seen in the window 1 of the shell Insert the voltage selector shell 3 together with the voltage selector 6 and fuses 7 back into the receptacle in the instrument and press lightly until it locks Check again if the right setting shows in the window 1 Instructions for Use V 2 7 RevA English 04 2011 4 Instrument Setup 4 3 3 Connecting the power cable 1 The instrument is supplied together with various country specific mains cables The instrument may only be connected to the mains with the cable supplied together with the instrument and it may only be connected to grounded sockets e Before connecting the mains cable check if the mains switch 2 at the rear of the instru ment is disconnected 0 e Outofthe selection of cables select the one with the plug that fits into your laboratory s wall outlets 2 D e Connect the mains ca
22. with aluminum containers instead of tf glass containers In aluminum containers the level indications marks are located on the inside of the container When filling the container make sure the filling Min level is not below the minimum or above the max imum level indication mark Fig 8 Leica TP1020 23 3 Instrument Components and Specifications 3 6 4 Wax bath Fig 9 Holding capacity 1 81 Nominal supply voltages 230 V AC 50 60 Hz Nominal capacity heating 150 VA Temperature setting range 45 65 C 3 K Excess temperature switch off mechanism at 85 C 5 K 24 If the actual temperature rises above the range of normal working temperature an excess tem perature switch off mechanism responds The wax bath heating is disconnected The yellow sig nal lamp is extinguished Use of the wax bath can be resumed only after a cooling down period For cooling down disconnect the wax bath connec tor plug from the socket at the rear of the instru ment or switch off the main switch The wax baths are equipped with heating While the heating is functioning the yellow pilot lamp lights 1 The handle 2 is insulated The outside wall 3 is likewise thermally insulated However it be comes hot to the touch in heating operation De spite this burn hazard exists when touching it Caution The interior container 4 of the wax bath becomess very hot when the heating function
23. 00 Ps Get D E P3 PROG OG 6 2 5 Activating the vacuum function instruments with vacuum function only 0h00 O Leica TP1020 e Press ARROW RIGHT to move the cursor to the next entry position The cursor blinks atthe station number data entry position e Select the desired station number by press ing PLUS or MINUS e Press ARROW RIGHT to move the cursor to the next entry position The cursor blinks atthe two positions next to the V for vacuum function e Toactivate the vacuum press PLUS e To deactivate the vacuum press MINUS A The Leica vacuum accessories for enhanced tissue infiltrations may ONLY be used with aluminum con tainers Glass beakers used in combination with the vacuum accessories have a high hazard potential when damaged 45 6 Programming the Instrument 6 2 6 Selecting infiltration times per station 0 hoo e Press ARROW RIGHT to move the cursor to the next entry position The cursor blinks at the hour entry digits e Press PLUS or MINUS respectively to enter the hours e Press ARROW RIGHT to move the cursor to the next entry position D SIE SC The cursor flashes on both digits destined for entering the minutes For stations you wish to leave out of e Press PLUS or MINUS to enter the minutes your program select an infiltration time of 0h 00 min me Setting range 0 hrs 05 min to 99 hrs 59 mi
24. 585 1 Tubing band clamp 14 0422 31973 Type 8 Two basket operation double loading standard tissue baskets With vacuum without fume control system Connection for optional third wax bath includes second tissue basket carrier 1 Basic instrument 14 0422 31414 9 Aluminum reagent containers for vacuum 14 0422 42545 9 Reagent container carriers 14 0422 42545 3 Wax baths 1 8 liter 14 0422 30665 2 Standard tissue baskets 14 0422 30585 Type 9 Basic Instrument for processing with chloroform as clearing reagent 1 Basic instrument 14 0422 42238 12 Glass beakers with handle 14 0422 42545 2 Wax baths for chloroform 14 0422 32001 1 Standard tissue basket 14 0422 30585 16 Instructions for Use V 2 7 Rev A English 04 2011 3 Instrument Components and Specifications Standard delivery continued Type 10 One basket operation standard tissue basket With fume control system and accessories 1 Basic instrument 12 Glass beakers with handle 2 Wax baths 1 8 liter 1 Standard tissue basket 1 Tubing band clamp 1 Activated carbon filter Standard 1 Activated carbon filter for formaldehyde 1 Exhaust hose diameter 50 mm 4 m long Type 11 1491020 US01 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 31973 14 0422 30673 14 0422 30674 14 0422 31975 One basket operation standard tissue basket With vacuum fume control system and accessories 1 Basic instrument 10 Aluminum reagent containers for vacuum 10 Reagent containe
25. D 69226 Nussloch Germany Phone 49 0 6224 143 0 Fax 49 0 6224 143 268 Internet http www leica microsystems com Leica TP1020 These are determined only by the contract provi sions agreed between ourselves and our cus tomers Leica reserves the right to change technical specifications as well as manufacturing process es without prior notice Only in this way is it pos sible to continuously improve the technology and manufacturing techniques used in our products This document is protected under copyright laws Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photo copy microfiche web cam or other methods including any electronic systems and media requires express prior permission in writing by Leica Biosystems Nussloch GmbH For the instrument serial number and year of manufacture please refer to the name plate at the back of the instrument Leica Biosystems Nussloch GmbH Table of contents Important Informati n SEENEN EE 7 CULLA EEE 8 2 1 Safety NOS an 8 22 ET aaa ala 8 Instrument Components and Specifications een 12 3 1 Technical data rcr aaa aan 12 3 2 Description of the instrument ww 4 Bid Standard CSlIVGRY sscesisessescoccsnccuccscerdecsessoscescsuscascvecvnsctessnacecceuscducsnsndassn hdadesasbecascsesvascsnstescastses Dat 3 4 General overview
26. H Processing station Il 36 Fig 20 The standard display shows the respective number of the station in which above which the holder for the specimen basket is currently locat ed At the same time the display also reads real time 24hour clock The colon between hours and minutes is blinking The display shows all tissue processing parame ters as well as the individual programs Tissue baskets and processing station are displayed with symbols In addition to programming features other useful data is displayed such as program duration and end of processing Also displayed are warning codes W 01 W 06 to ensure trouble free processing and error codes E 01 E 13 which indicate instrument or data entry errors Instructions for Use V 2 7 RevA English 04 2011 5 The User Interface Key functions Programming control panel locking time setting up and down movement of the tissue basket Programming button PROG To call and quit the programming mode in which programs are set up edited and altered and to display a program which is currently activated Lock button KEY To lock all control panel functions as protection against accidental changes of programmed pa rameters e To activate and quit the lock function press KEY for 5 seconds LOCKED will be displayed LJo 4 LOCKED Llo4 10 20 Leica TP1020 After 10 seconds the display returns to standard
27. Leica TP1020 with additional wax bath optional accessory 18 3 5 General overview Leica TP1020 with fume Control system c secseecsecseesesseesseeseesseeseeseesneesesneenss 20 BG AGCCESSOMES Supplied ee eeteteielteen eerste i Ri 3 6 1 Standard tissue basket e 30 2 Glass beaker witht holder iii iii inci 22 3 6 3 Aluminum container with holder standard outfit in instruments with vacuum function or available as optional accessory for other types iii 23 364 WX E EE 24 Instrument seu 4 1 Unpacking instructions 4 2 Installation site requirements 8 AS Electrical COMM re LEE 4 3 1 Checking the voltage Selector setting iii 29 4 3 2 Adjusting the voltage selector Se 4 3 3 Connecting the power TO AA Switching on the Instrument ieri 45 Setting the time 4 6 Instalimgithe aGCessories i e aaa ici 46 1 lnstallingthe wax baths ica ieri elia 4 6 2 Connecting the wax baths 7 4 6 3 Fastening the wax bath connection cables instruments with fume control system only 35 46 4 Installing the reagent stations ENEE The User In E Programming the Instrument iii 6 1 GeneraldEseriptiom ari coves c evarsatebeebscvsasusescecbenias 6 1 1 Special characteristics of two basket programs o BI Setting up editing progr aMS weccseissccoscssscaccsncrascsncsuecoutsascvetsncesecevcodetescesecsscobcsnecedesbecsctebecvetsucr eteschustea
28. al service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Date of delivery Decommissioning and disposal Leica TP1020 The instrument or parts of the instrument must be disposed of in compliance with the local laws 97
29. always be kept near the instrument i H additional requirements for acci dent prevention and environmental protection exceeding the scope of these Instructions for Use are im posed by laws regulations of the country of operation these Instruc tions for Use must be supplemented by appropriate instructions to ensure compliance with such requirements This instrument has been built and tested in accordance with the following safety regulations on electrical measuring control regulating and laboratory devices In order to maintain this condition and to ensure safe operation the operator must observe the instructions and warnings contained in these In structions for Use i For current information about applica ble standards please refer to the CE declaration of conformity on our inter net site www leica microsystems com A instrument s internal components The protective devices on both instrument and accessories may neither be removed nor modi fied Only authorized and qualified service personnel may repair the instrument and access the 2 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the institu tion which owns the instrument and in addition the designated personnel who operates services or repairs the instrument To ensure
30. ave the V button for vacuum on the control panel however this key is not functional V is not displayed Of course a vacuum function cannot be activated with the V buttons in those cases A Instruments equipped with vacuum function may only be operated with the aluminum contain ers supplied together with the instrument Glass beakers used in combination with the vacuum accessories have a high hazard potential when damaged In the manual processing mode pressing a but ton activates deactivates the vacuum function e Press V to activate vacuum The display reads VAC vacuum The pump noise diminishes while vacuum is generated e To deactivate the vacuum press V again VAC disappears from the display The vacuum is deactivated While the container is being ventilated a slight hissing noise can be heard Only after the station has been completely ventilated can the tissue basket be lifted out of it Llo3 vac 09 17 Llo 3 WAIT 09 18 Leica TP1020 55 9 Operating the Instrument in the Automatic Processing Mode 9 1 Starting a program A program can either be started immediately or at a later point in time using the delay function 9 1 1 Immediate start station number vacuum on off and infiltration time of the basket in the starting contain er Therefore check the parameters on display and decide if you actually wish to start the displayed prog
31. ble to the correspond ing socket 8 at the rear of the instrument and plug the mains cable into the wall outlet The instrument is ready to be switched on Fig 16 Leica TP1020 31 4 instrument Setup 4 4 Switching on the instrument TP1020 V 45 Setting the time 32 e Switch on the instrument with the mains switch on the right side at the rear of the in strument The instrument does a startup run Next the instrument name and software version e g V 1 00 are displayed This reading disappears after about 10 seconds Next the number of the processing station where the basket holder is located and the real time indication are displayed e Press CLOCK The cursor blinks at the hour indication e To adjust the hours press PLUS or MINUS respectively e Press ARROW RIGHT The cursor jumps to the minute indication e To adjust the minutes press PLUS or MINUS respectively O a e To finish setting the time press CLOCK again Instructions for Use V 2 7 RevA English 04 2011 4 Instrument Setup 46 Installing the accessories e Press ARROW UP to lift the carousel To install and remove the reagent con tainers and wax baths the instrument can be rotated on the bench This en sures good access to all stations at any time
32. borted e To stop a processing cycle in progress press STOP The carousel up and down movement stops immediately The display reads STOP With this the instrument is asking you whether you really want to abort the processing cycle currently in progress To resume processing press START Processing will continue as programmed Instructions for Use V 2 7 RevA English 04 2011 9 Operating the Instrument in the Automatic Processing Mode Stopping or aborting a process continued STOP 9 7 End of an automatic process Ll1 2 DONE Leica TP1020 If there is an emergency an automatic process ing cycle can be stopped immediately and in a subsequent step entirely aborted Processing will continue as programmed e To abort the process press STOP again This will terminate the specimen processing def initely without any option to resume processing The tissue basket remains immersed in the cur rent station and has to be removed in the manual processing mode The termination of automatic specimen process ing is indicated in the display supported by an audible signal that is repeated in 30 second inter vals The position of the specimen basket and DONE appear in the display e Press any key to confirm the message and turn off the sound signal The specimens can now be removed in the manual processing mode 65 9 Operating the Instrument in the Automatic P
33. d 2 off of the crate e Remove the Instructions for Use 3 and packing list e Remove all accessories 4 from the compartments of the cardboard grid 5 e When all parts have been removed lift them upwards and out and remove the intermediate bottom 6 underneath e Lift the outer carton ring 7 upwards and out Fig 10a 25 4 Instrument Setup 11 not visible 10 Fig 10b 26 i For safe transport of the instrument we rec Remove the wooden ledge 8 sideways from the interior box 9 Remove the interior box 9 in an upward move ment To lift up the instrument using 2 persons take it by the reagent container platform or by the lower end of the housing see Fig 10b cl e below and lift it from the base plate 10 Install the instrument on a stable laboratory bench Four rollers 11 under the instrument housing per mit rotating the instrument on the bench Remove the sponge rubber transport safety device 12 from under the carousel cover 13 Depending on the instrument version 4 foam trans port anchors 14 may have to be removed between the Plexiglas plates 15 When transporting the instrument do NOT lift it up by the carousel cover ommend keeping the original packaging Instructions for Use V 2 7 RevA English 04 2011 4 Instrument Setup Unpacking continued When transporting the instrument do not lift it up by the car
34. d continuously In addition 5 minutes after last pressing a key a double sound signal reminds the user the run is still paused The sound signal will recur in 5 minute intervals until processing is resumed When in PAUSE the buttons for manual opera tion become functional e g to lift the tissue bas ket out of a station to add specimens The carou sel can also be rotated to move the basket to any processing station see chapter 8 Operating the Instrument in the Manual Processing Mode Leica TP1020 63 9 Operating the Instrument in the Automatic Processing Mode 9 5 Resuming a paused process PAUSE CONT 9 6 Stopping or terminating a process STOP Llor vac 0h55 P8 STOP Lloi vac 0h55 i A sl J P 8 10 19 To reassume processing press PAUSE CONT again Pressing this key will lower the basket into the station Processing continues as programmed While a run is paused the infiltration time count down in the actual station will also be interrupt ed When processing is resumed the remaining infiltration time in the station will be carried out without interruption Thus the end of run time of a process will be postponed by the length of time the process was paused The display reading will automatically be updat ed showing the new end of run time If there is an emergency an automatic process ing cycle can be stopped immediately and in a subsequent step entirely a
35. desecaes 6 2 1 Selecting programming MOE oeren 6 2 2 Selecting the program E 6 2 3 Selecting the number of baskets c cccsecssssssssseseessessecssesnecsnssnecssessessessuesuecsnssneesessneeseesncsueesessneeseesneenes 6 2 4 Selecting the starting Station ccssssssssssssssssssssssssessssssssecsssssscsssssessessuscsessuscasssasssessesssessssseeseseesssess 6 2 5 Activating the vacuum function instruments with vacuum function oh 45 Instructions for Use V 2 7 Rev A English 04 2011 Table of contents 10 11 12 6 2 6 Selecting infiltration times per station cceceecsecssecsecssessecssesneeseesseesessuesnecsnssneessesneeseesnssnseseesneeseesnesees 46 6 2 7 Displaying total program Guration iListas painaa deda i a 4 6 2 8 Leaving the programming mode EEN 4 Preparations for Tissue Processing cc 48 7 1 Filling the reagent stations 7 2 Fillinigithewax dd E 7 3 Inserting the tissue basket iiii nakani aaai i iaaini aiaiai 51 Operating the Instrument in the Manual Processing Mode seen 53 8 1 Lifting and lowering the tissue basket 53 8 2 Moving the tissue basket to the next station iii 54 8 3 Activating the vacuum function instruments with vacuum function only 11111110 55 Operating the Instrument in the Automatic Processing Mode senn 56 9 1 ell Nels 56 9 1 1 Immediate start 9 2 Delayed Start EE 9 2 Editing and modifying programs while
36. djust voltage selection the fuses back into the receptacle in the instrument and press lightly until it locks e Remove the fuses 7 e Check ifthe setting that shows in the window e Insert replacement fuses of the same type 1 corresponds to the nominal voltage in your laboratory 80 Instructions for Use V 2 7 RevA English 04 2011 13 Optional Accessories 13 1 Three level tissue basket Fig 26 Holding capacity 20 cassettes per level The three level tissue basket consists of a holder 1 in which the three levels 2 for the cassettes are stacked The upper level is closed with a lid 3 Leica TP1020 81 13 Optional Accessories 13 2 Basket removal device Fig 27 82 The basketremovaldevice with drip tray makes it easy to remove the hot dripping tissue basket at the end of the processing cycle The rubber insert 4 of the drip tray can be removed for cleaning Grasp the basket removal device atthe black handle and insert the dripping tray under the tissue basket Insert the metal hook 1 under the notch at the bottom of the tissue basket To liftthe hook 2 press the spring 3 down wards with your thumb Lock the hook 2 into the upper rim of the tis sue basket and release the spring Lift the basket slightly and remove it from the basket holder in a horizontal movement Instructions for Use V 2 7 RevA English 04 2011 13 Optional Acce
37. e 2 Wax baths 1 8 liter 1 Standard tissue basket 1 Tubing band clamp Leica TP1020 Order No 14 0422 30543 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 31418 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 30536 14 0422 42545 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 30537 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 31973 15 3 Instrument Components and Specifications Standard delivery continued Type 5 Order No One basket operation standard tissue basket With vacuum with fume control system 1 Basic instrument with vacuum and extraction 14 0422 30535 10 Aluminum reagent containers for vacuum 14 0422 42545 10 Reagent container carriers 14 0422 42545 2 Wax baths 1 8 liter 14 0422 30665 1 Standard tissue basket 14 0422 30585 1 Tubing band clamp 14 0422 31973 Type 6 Two basket operation double loading standard tissue baskets Without vacuum with fume control system 1 Basic instrument with extraction 14 0422 31416 11 Glass beakers with handle 14 0422 42545 3 Wax baths 1 8 liter 14 0422 30665 2 Standard tissue baskets 14 0422 30585 1 Tubing band clamp 14 0422 31973 Type 7 Two basket operation double loading standard tissue baskets With vacuum with fume control system 1 Basic instrument 14 0422 31412 9 Aluminum reagent containers for vacuum 14 0422 42545 9 Reagent container carriers 14 0422 42545 3 Wax baths 1 8 liter 14 0422 30665 2 Standard tissue baskets 14 0422 30
38. e instrument 86 Instructions for Use V 2 7 Rev A English 04 2011 13 Optional Accessories 13 5 Inserting the activated carbon filters optional accessory for instruments with fume control system gt Instruments with fume control system can be equipped with two activated carbon filters 1 One filter is specific to formaldehyde whilst the other takes up other solvents The working life of the activated carbon filters is dependent on the reagent concentrations actually used instrument running time and ambient temperature Fig 32 Unpack the filters To open the lid 3 of the receptacle on the left side of the instrument loosen the two knurled knobs Fold down the lid 3 Insert the activated carbon filter for oth er solvents 1 in the lower guide rail and push until itis completely inserted Insert the activated carbon filter for formaldehyde 2 inthe upper guide rail and push until itis completely inserted e Fold up the lid and lock by tightening the two knurled knobs A Warning Fire hazard If a filter becomes saturated with solvent there is a potential fire risk It is important that the activated carbon filters are changed at factory recommended intervals Generally the activated carbon filters have to be replaced every four months Leica TP1020 87 14 Ordering information 14 1 Accessories Standard tissue E 14 0422 30585 Thre
39. e level tissue basket 14 0422 30547 Single level compartment for three level tissue basket E 14 0422 30622 Lid for three level tissue basket iii 14 0422 30623 Basket removal device with drip tray cccccecssecsesessesescsesecsesecsesessessesesaseeseeeeseseseees 14 0422 30637 Glass WEAK ONG eer 14 0424 60429 Beaker carrier eer 14 0422 30671 Aluminum container RH 14 0422 32166 1 Station holdertorWaxXbatli ac cara 14 0422 30571 Wax bath Leer 14 0422 30665 Wax bath resistant to chloroform 1 81 adjustable up to 70 C 14 0422 32001 Screwdriver for wax bath siiiswiiacadnnimnaiisnndinatcenboninundinnanncennala 14 0170 10702 Fume outlet tube solvent resistant 2 me 14 0422 31974 Fume outlet tube solvent resistant 4 M Jc cora 14 0422 31975 Instructions for Use V 2 7 Rev A English 04 2011 15 Appendix 15 1 Modifying the instrument configuration The instrument has a factory set standard con Configuration Signal level 4 i For this menu item four different settings can be selected Setting 4 is factory preset Setting 1 is the lowest level The next higher level always contains all the parameters of the next preceding lev el plus the additional signals as listed on this page Leica TP1020 figuration which can be modified by the user if desired Modifications can be done in the configuration menu which consists of 4 differen
40. e with the instrument 92 The following reagents are safe for use with the TP1020 Important Using other than the reagents listed below can cause damage to the instrument or to parts of the instrument Fixation Formalin buffered or unbuffered Picric acid Dehydration Ethanol Isopropyl alcohol Methanol Butyl alcohol Industrial alcohol Clearing Xylene and xylene substitutes Toluene Benzene Acetone Important Chloroform These substances are permitted only with instrument Trichlorethane fl variant 9 For additional information refer to Chap 3 3 Standard delivery page 16 Paraffin Paraffin Instructions for Use V 2 7 Rev A English 04 2011 15 Appendix 15 4 Factory set programs Program No 6 1 basket Station Reagent VAC Duration 1 V 0h15 2 V 0h15 3 V 0h15 4 V 0h15 5 V 0h15 6 V 0h15 7 V 0h15 8 V 0h15 9 V 0h15 10 V 0h15 11 Paraffin V 0h15 12 Paraffin V 0h15 Program No 8 2 baskets Station Reagent VAC Duration 2 V 0h15 3 V 0h15 4 V 0h15 5 V 0h15 6 V 0h15 7 V 0h15 8 V 0h15 9 V 0h15 10 V 0h15 11 Paraffin V 0h15 12 Paraffin V 0h15 Leica TP1020 93 15 Appendix 15 4 Factory set programs continued Program No 7 1 basket Station Reagent VAC Duration 1 V 1h00 2 V 1h00 3 V 1h00 4 V 1h00 5 V 1h00 6 V 1h00 7 V 1h00 8 V 1h00 9 V 1h00 10 V 1h00 11 Paraffin V 1h00 12 Paraffin V 1h00 Program No 9 2 basket Station Reagent VAC Duration 2 V
41. ed Do not touch the gray upper rim of the containers with your hands Risk of injury Caution when handling hot paraffin Risk of injury A Spilled reagents have to be wiped away immediately In case of long term exposure the e Disconnect the wax bath plugs slightly lift the wax bath lids and remove the paraffin containers Empty the wax baths completely before the paraffin cools When cooling down the paraffin contracts which can cause damage to the wax bath e Lower the carousel in the manual processing mode A Before cleaning the instrument disconnect the mains switch To clean the painted surfaces the container platform and the control panel do not use solvents containing acetone or xylene neither use abrasive cleaning powders Only mild household detergents may be used The lacquered surfaces and the control panel are not resistant xylene or acetone e Remove residual paraffin from the station holders lid seals and lid holders e Use a soft plastic spatula for removing the paraffin Leica TP1020 75 11 Cleaning When cleaning the instrument no liquid may enter in contact with any of the electrical con nections or the interior of the instrument e Use a moist cleaning cloth to clean platform lacquered instrument surfaces and control panel e Clean the rims of the glass or aluminum reagent containers and of the wax baths 1 The glass and aluminum containers
42. ed the green diode in the THREE ARROW button is lighted e Toswitch off the function press the THREE ARROW button The diode is extinguished the function is switched off It can however be turned back on at any time e To reactivate the function press the THREE ARROW button once again LI If the tissue basket is located in a wax bath that contains solid 1 paraffin the instrument disconnects the up down function auto matically Instructions for Use V 2 7 Rev A English 04 2011 5 The User Interface Start pause resume stop and abort automatic tissue processing cycles START PAUSE CONT HH Uoi vac P4 PAUSE 0h12 STOP 0h55 H ln VAC P8 STOP Leica TP1020 START button To immediately start processing or to insert de layed starting parameters for automatic process ing after having selected a program PAUSE CONT button To pause and resume an automatic processing cycle When the function is activated the display reads PAUSE In addition 5 minutes after last pressing a button a double sound signal will remind the user that processing is still paused This signal will repeat itself every 5 minutes until the auto matic processing cycle is resumed When automatic processing is paused the but tons for manual processing become functional so that e g the tissue basket can be lifted out of the station to add tissue samples o
43. ee Chapter 13 is displayed while you try to lower the basket into one of the wax baths check whether the paraffin is actually liquefied Leica TP1020 If yes you may carry on and lower the basket into or lift it out of the wax bath 53 8 Operating the Instrument in the Manual Processing Mode Lifting and lowering the tissue basket continued e To lower the basket press KEY and ARROW DOWN simultaneously Or e To lift the basket press KEY and ARROW UP simultaneously 8 2 Moving the tissue basket to the next station To transport the tissue basket from one station to E ei the next the CIRCLE ARROW button has to be pressed The movement can only be carried out in single steps and clockwise e Press CIRCLE ARROW LJo1 The basket is moved to the next station WAIT ew AIT alal lzi and two blinking arrows indicating in which di m rection the basket is moving are displayed The carousel will stop when the basket is above the next station without lowering the basket into the station a e To move on to the next station press CIRCLE ARROW again 54 Instructions for Use V 2 7 Rev A English 04 2011 8 Operating the Instrument in the Manual Processing Mode 8 3 Activating the vacuum function instruments with vacuum function only bed The Leica TP1020 is available in different types with or without vacuum function Instru 1 ments without vacuum function also h
44. er clockwise Instructions for Use V 2 7 Rev A English 04 2011 13 Optional Accessories Basket holder for second tissue basket continued i The instrument standard configuration will then have to be changed accordingly to acknowl edge that a third wax bath has been connected see Chapter 15 WE Fig 30 Installing the station holder e Use a screw driver to lift the reagent station holder out of the platform e Insert the wax bath holder and drive in with a plastic hammer Leica TP1020 Connecting the third wax bath e Pull off the cover cap from the socket no 10 e Insert the plug 1 of the third wax bath into socket no 10 and secure with the clip 2 85 13 Optional Accessories 13 4 Connecting the fume outlet tube optional accessory for instruments with fume control system length of 2 or 4 meters the instrument can be connected to a cen tral fume extraction system The fume outlet tube can also be used to conduct solvent fumes outside a building i Via a solvent resistant fume outlet tube which is available in a Y V E 0 ii l i E An See N i ZEN ia f N 1 Jz _ Ca J ua AA Le Fig 31 Installing the fume outlet tube e Put the tube 1 over the connecting piece on the left side of the housing and fasten by means of the hose clamp 2 that is supplied with th
45. ginally set up 73 10 Warning and Error Codes Troubleshooting 10 4 ABORT message Loos III ABORT 0h47 START START 0 ole ZIS im t a w gt _ D m i a E 3 74 ABORT is displayed after quitting an error code emitted due to instrument malfunction When con firming such an error message processing is aborted Atthis point processing can be restarted from the beginning To skip the stations that had already been covered during the previous aborted run proceed as follows e Press START e Tostartthe currently displayed program press START again This will cause the instrument to step by step move the basket to the programmed starting station Before reaching that position e Press PAUSE CONT to switch to the manual processing mode e Press CIRCLE ARROW to move the basket to the station where processing was aborted e Press ARROW DOWN to lower the basket e To leave the manual processing mode press PAUSE CONT again Processing will then continue where it had previ ously been aborted Instructions for Use V 2 7 Rev A English 04 2011 11 Cleaning e Lift the carousel in the manual processing mode e Remove all station containers from the platform instrument surfaces are only conditionally resistant to solvents Caution The interior containers of the wax baths become very hot when the heating func tion is activat
46. he corresponding function keys on the control panel Leica TP1020 51 7 Preparations for Tissue Processing Inserting the tissue basket continued two blinking arrows which indicate the direction of movement in each particular case When the carousel has completely come to a halt this indication will disappear from the display D While the carousel is moving the display reads WAIT and shows To avoid reagent carryover as far as possible in the manual mode allow for a sufficient dripping time 52 Instructions for Use V 2 7 Rev A English 04 2011 8 Operating the Instrument in the Manual Processing Mode 8 1 Lifting and lowering the tissue basket A Use caution when lowering the carousel Keep your fingers out of the space between the container lid and the upper rim of the container HO DI LJo1 VYWaitT 09 20 in W 02 Pressing the corresponding button the basket can be lifted out of a station or lowered into one e To liftthe basket press ARROW UP e To lower the basket press ARROW DOWN The up or down movement is carried out entirely and a corresponding message is displayed while the action is in progress e To interrupt an up or down movement before itis completed press one of the two ARROW keys again The movement will stop immediately when press ing the button D If one of the warning codes W 01 W 03 s
47. he main switch and next switch it back on Try again Call Technical Service Error code E 07 Clock component defective Call Technical Service Error code E 08 Wax bath 1 Error code E 09 Wax bath 2 Error code E 10 Wax bath 3 Leica TP1020 Wax bath not connected Wax bath possibly connected to wrong socket Excess temperature switch off mech anism has responded Wax bath defective Control unit defective Connect wax bath Check if wax bath is connected to the appropriate socket Correct if necessary Disconnect the wax bath con nection plug from the socket at the rear of the instrument Let wax bath cool down Reconnect wax bath If the yellow pilot lamp lights use of the wax bath may be continued Exchange wax bath Call Technical Service 69 10 Warning and Error Codes Troubleshooting List of error codes continued Press any key to confirm error Error code E 11 Loss of process data message ABORT will be dis played processing cycle will be aborted Restart processing cycle Ifthe same problem recurs call Technical Service Error code E 12 Loss of program settings Press any key to confirm error message ABORT will be dis played processing cycle will be aborted Restart processing cycle Ifthe same problem recurs call Technical Service Error code E 13 Erroneous data entry for delayed Correct errone
48. hile the carousel is in the upper end position ARROW UP and ARROW DOWN button for vertical carousel movement Lift and lower respectively the carousel to with draw the basket from a processing station or insert a basket into a processing station To halt an up or down movement while still in progress push one of the two buttons again The movement will stop immediately when pressing the button Vacuum function variant 2 and 4 To connect disconnect the vacuum function in the manual processing mode Instructions for Use V 2 7 Rev A English 04 2011 5 The User Interface These buttons are equipped with a repeat function If one of the ARROW is pressed for a while the cursor jumps to the respectively next data entry position If one of the PLUS MINUS buttons is pressed for a while the displayed numerical value is continuously increased or decreased The Leica TP1020 is available with and without a vacuum function For the variants without vacuum func tion no V appears in the display Of course a vacuum function cannot be activated with the V buttons in those Cases Leica TP1020 Programming mode buttons These buttons are used to enter or alter parame ters when programming ARROW LEFT ARROW RIGHT buttons cursor To move the cursor in the direction of the arrow to the respectively next data entry position e When pressing one of the ARROW buttons
49. is not part of the program programmed infiltration time 0 h 00 min in stead of POWER FAILURE the message WRONG STATION is displayed In addition when mains power is restored an uninterrupted sound signal is emitted The upper line of the display shows how long the basket remained in the wrong station due to the power failure In the example shown here the basket remained in station no 4 for 16 minutes By pressing any key the display reading will switch back to normal and the sound signal will be turned off Once mains power is restored processing will be reassumed as programmed The basket will be moved from the wrong station to the next station that is part of the current program 71 10 Warning and Error Codes Troubleshooting 10 3 2 Measures to remove specimen material in case of prolonged power failure i In case of a prolonged power failure the tissue can be removed from the instrument manually and also be relo cated to the next station by entirely manual operation Switch off the mains switch Remove the crank handle 1 fromthe clip and insert it into the access port 2 on the left side of the instrument Removing the tissue basket e Liftthe carousel rotating the crank handle in the direction of the arrow until the lower end of the basket is above the rim of the pro cessing station e Hang on to the crank handle to keep it securely in its position and rem
50. ivate the delay function press START START o The warning codes W 04 W 06 see Chapter 7 are displayed for programs of less than 8 hours 1 duration from start until the basket reaches the first wax bath Check if the available amount of time is sufficient for the paraffin to liquefy completely If this is not ensured fill the wax baths with liquid paraffin e To override the warning codes and start pro cessing press KEY and START simultaneously The program will be started atthe selected delay time e To quit the display indication press CLOCK e again 60 Instructions for Use V 2 7 RevA English 04 2011 9 Operating the Instrument in the Automatic Processing Mode Locking the key functions i To protect program settings against unintended deletion or alter ations the key functions of the control panel can be locked e To lock the panel press KEY for 5 seconds LOCKED will be displayed After 10 seconds the display will return to standard reading Every time a button is pressed LOCKED will be displayed again e To unlock the control panel functions press KEY again for 5 seconds Leica TP1020 61 9 Operating the Instrument in the Automatic Processing Mode 92 Editing and modifying programs while a processing cycle is in progress in progress All programs can be edited and changed with the exception of the program currently in progress A program
51. ket program permanently If the second basket is deleted the starting con tainer and the infiltration times remain as set for the two basket program They have to be reen tered as desired for each individual station 43 6 Programming the Instrument 6 2 Setting up editing programs 6 2 1 Selecting programming mode PROG Lloi v P3 PROG 0h50 6 2 2 Selecting the program Lloi v Ny P3 PROG BIR 6 2 3 Selecting the number of baskets ES P 3 0 h50 V 0h00 O PROG 44 e To select the programming mode push the PROG key The following parameters are displayed Number of baskets H or H HH Number of processing station L1 12 Vacuum ONT or OFF Tissue infiltration time in the displayed station 0 h 50 min Program number Programming mode P1 P9 PROG The cursor blinks at the program number e Select the desired program number by pressing PLUS or MINUS e Push the ARROW LEFT button to move the cursor to the upper line The cursor blinks next to the first basket symbol e To change the number of baskets from 1 to 2 press PLUS e To change the number of baskets from 2 to 1 press MINUS With this the infiltration for the tissue baskets will be the same in all stations Instructions for Use V 2 7 RevA English 04 2011 6 Programming the Instrument 6 2 4 Selecting the starting station LI v 0h
52. luding accessories ENEE 60 kg Net weight including packaging material e 116 kg Wax baths Quantity E 2 optionally 3 Volume ee EE 1 8 Nominalvoltage ariani 230 V AC 50 60 Hz Nominal capacity per station iiii n nA ANRE R HORNNES ASES RERNENR NENS 150 VA Temperature setting range i 45 C 65 C 3 K Excess temperature SHUtCOWN EE 85 C 5K 12 Instructions for Use V 2 7 Rev A English 04 2011 3 Instrument Components and Specifications Technical Data continued Reagent stations Licei 10 9 when 3rd wax bath is installed VOUM LE 1 81 tissue basket AC VARO AE E EEA NE 1 optionally 2 HAdINg Ga atelier 100 cassettes optionally 200 Programs LIE sailor 9 freely selectable Programmable infiltration time per station 99h 59 min Start CGAY E 9 days Dripping Bel EE 60 seconds Vacuum function types 3 5 7 8 and 11 only see Chapter 3 3 Pressurediletonidlic a Max 500hPa 10 approx 0 5bar Leica TP1020 13 3 Instrument Components and Specifications 3 2 14 Description of the instrument The Leica TP1020 is an automatic tissue processor designed for laboratory applications It is used for the fixation dehydration and infiltration of histo logical tissue samples with fixatives alcohol solvents and paraffin wax The reagent stations numbered 1 10 are used to contain reagents Station 10 may be replaced with an optional third wax bath Stations 11 12
53. m press the CURSOR button The next standard setting is displayed Configuration ProgPreset ProgPreset Set programs 6 9 to default values 90 Instructions for Use V 2 7 RevA English 04 2011 15 Appendix Modifying the instrument configuration continued Programs no 6 9 come with a num Standard setting is NO 1 ber of factory preset standard values however these may be altered by the e To change the setting press the PLUS key user When answering YES programs no 6 9 are set to standard values and programs no 1 5 are deleted e For the next menu item press the CURSOR Configuration button V Sy stemReset The next standard setting is displayed 1 A system reset will set real time to SystemReset Resetting certain 0 00 and maximum heating time for the values to default values wax baths to 8 hours The Sound Signal Level setting and the number Standard setting is NO of wax baths selected in the standard configuration menu will remain unal tered by a system fesat e To change the setting press the PLUS key When answering YES the system clock is set at 0 00 and the maximum heating time for the wax baths is set at 8 hours No other settings are altered Leaving the configuration menu e To leave the configuration menu press PROG Leica TP1020 91 15 15 2 Reagents appropriate for us
54. n Press ARROW RIGHT to quickly jump to the next o hoo Station for entering the desired parameters vac uum and infiltration time Press PLUS MINUS to enter the desired parameters In two basket programs infiltration time is the same for all stations m DI 46 Instructions for Use V 2 7 RevA English 04 2011 6 Programming the Instrument 6 2 7 Displaying total program duration Once a program is set up total run time can be displayed e Press CLOCK to display the total run time of RH the program The total duration displayed here is 1 day 0 hours EA d Lloi vac SEH and 12 minutes e To quit the display indication press CLOCK RH again P TOTAL 1d00h12 While you are in the programming mode you cannot start a program Prior to starting a 1 program you must quit the programming mode 6 2 8 Leaving the programming mode To end programming you have to leave the programming mode e Press PROG to quit programming A Warning Use caution when handling solvents Make sure the premises are adequately venti lated Explosion hazard Always observe worker s protection rules and use adequate protective gear gloves laborato ry coats While operating the instrument no liquid may enter in contact with any of the electrical con nections or the interior of the instrument To remove and reinstall the reagent containers and wax baths the ins
55. n as the basket has arrived at the pro grammed starting station it will be lowered into that station Leica TP1020 57 9 Operating the Instrument in the Automatic Processing Mode Immediate start continued Lloi vac ER P 4 09 29 9 1 2 Delayed start START Lloi vac 1h Ga t P4 START 00 00 58 From that point on the remaining infiltration time of the basket in the current station is displayed While processing time is running the display reading is updated in one minute intervals i e you can always see exactly the remaining infil tration time for each station Select starting time e Press START The program which was used last is displayed Decide whether you want to start that particular program or select another one e To enter the desired starting time press ARROW RIGHT The display reads START and the cursor blinks at the data entry position for the days of delay 0 Start at the same day today 1 Start the next day tomorrow 2 Start in two days the day after tomorrow ett The delay function enables you to start a program e g during the week end so it will be completed by the time you return to work on Monday Instructions for Use V 2 7 RevA English 04 2011 9 Operating the Instrument in the Automatic Processing Mode Delayed start continued Llo vac P4 START 1 19 Llo
56. nding station holder and push the cable into the notch at the edge of the platform Check for each wax bath whether it is actually installed at same station number it is connected to at the rear of the instrument clean and undamaged The covers must close tightly as for instruments with vacuum function the vacuum would not be able to be built up 1 The wax bath rims and sealing rings of the lids always have to be Instructions for Use V 2 7 Rev A English 04 2011 7 Preparations for Tissue Processing 7 3 Inserting the tissue basket Use caution when lowering the carousel Keep your fingers out of the space between the container lid and the upper rim of the con tainer e yTy7 zs m_ Fig 22 Fillthe tissue cassettes or capsules into the tissue basket To hook in the tissue basket s the bas ket holder s should not be located over a wax bath Lift the carousel in the manual process ing mode Rotate the carousel so the basket holder 1 is located above the starting container for basket 1 Hook the tissue basket 2 into the bas ket holder as shown Lower the basket into the starting con tainer in the manual processing mode or start an automatic processing cycle The carousel may not be result from doing so rotated manually Severe damage will When working in the manual mode all carousel movements are activated through t
57. nt shields can be freely rotated and dis placed as required without being obstructed by the wax bath connection cables two magnetic clamps are provided on each of the cables to attach the cables to the housing After having mounted and connected the wax baths place the magnetic clamps on the side wall of the housing so that the cables are fixed in a position that ensures free movement of the fume containment shields 4 6 4 Installing the reagent stations o Sedi Kon HEET il eh foo ING nei Kl E gt SES J oY SSN 8 E AW Fig 19 Leica TP1020 Mount the reagent con tainers 1 glass or alu minum onto the station holders 2 of the platform 35 5 The User Interface Control panel E PAUSE 4 mm ca O 0000 0006 The control panel is an easy care key pad The individual keys are organized in four function al groups Pressing anykeywillbe acknowledged by an audible signal This function can be discon nected if required see Chapter 15 Display The display is a two line LCD indication each line with 16 single characters When the instrument is on the display is always illuminated fos 2
58. or Use The Leica TP1020 is a modular automated tissue proc essor designed forthe following laboratory applications e fixation e dehydration e paraffin wax infiltration of histological tissue specimens The Leica TP1020 must be operated exclusively with the reagents listed in chapter 15 A Any other use of the instrument is con sidered improper Failure to adhere to these instructions may result in an ac cident personal injury damage to the instrument or accessory equipment Instrument type All information provided in these Instructions for Use applies only to the instrument type indicated on the title page A name plate indicating the instrument serial number is attached to the back of the instrument feica SACE Model Fabr Nr 3740 03 2009 TP 1020 1 1 Kat Nr SEH ___100 120 230 240 MN ZOO VA Mie nostrani _ 50 60 Hz Leica Biosystems Nussioch GmbH D 69226 Nussoch 2 Safety A use of other Leica products Make sure to comply with the safety instructions and warnings in this chapter Make sure to read these instructions even if you are already familiar with the operation and 2 1 Safety notes These Instructions for Use includes important in formation related to the operating safety and maintenance of the instrument The Instructions for Use are an important part of the product and must be read carefully prior to startup and use and must
59. ore cleaning the instrument disconnect the mains switch Caution The interior containers of the wax baths become very hot when the heating function is activated Do not touch the gray upper rim of the containers with your hands Risk of injury Caution when handling hot paraffin Risk of injury When cleaning the instrument no liquid may enter in contact with any of the electrical con nections or the interior of the instrument Spilled reagents have to be wiped away immediately In case of long term exposure the in strument surfaces are only conditionally resistant to solvents To clean the painted surfaces the container platform and the control panel do not use sol vents containing acetone or xylene neither use abrasive cleaning powders Only mild household detergents may be used The lacquered surfaces and the control panel are not resistant to xylene or acetone A 10 Instructions for Use V 2 7 Rev A English 04 2011 2 Safety Maintenance e For purposes of maintenance or repair the instrument may only be opened by service technicians authorized by Leica e Before exchanging the fuses switch off the mains switch and unplug the instrument e Burnt out fuses may only be replaced by fuses of the same type and specification For appropriate brands and specifications see Chapter 3 Technical Data Wax baths e If the excess temperature repeatedly please call Leica Technical Service
60. ous data entry start function e g desired starting time would be earlier than current real time 70 Instructions for Use V 2 7 Rev A English 04 2011 10 Warning and Error Codes Troubleshooting 10 3 1 POWER FAILURE and WRONG STATION messages D Uoi 0h47 Sat ee be ort eet tet POWER FAILURE Ca aa TI PTT ot In case of a power failure the carou sel is lowered immediately into the station where the tissue basket hap pens to be located when the power failure occurs Once mains power is restored processing will be resumed as programmed Possible impairment of the processing cycle through the interruption is displayed i l In 0h16 iii ria WRONG ST TION T rere t et t St Leica TP1020 POWER FAILURE is displayed when mains pow er is restored after a power failure but only if the processing cycle in progress has been impaired by the power failure The display reading is accompanied by a sound signal which intends to attract the user s atten tion to the malfunction so he she can take appro priate measures to rescue the specimens The upper line of the display shows the surplus time the basket remained immersed and the sta tion number where it was immersed due to the power failure In the example shown here the infiltration time in station no 3 was exceeded by 47 minutes If due to the power failure the basket is lowered into a station which
61. ousel cover e To lift up the instrument take it by the reagent container platform or by the lower end of the housing Fig 11 and lift it from the base plate 8 e Install the instrument on a stable laboratory bench Four rollers 12 under the instrument housing permit rotating the instrument on the bench e Remove the sponge rubber transport safety device 9 from under the carousel cover 11 LI For further secure transport of the 1 instrument we recommend you keep the original packaging material Fig 11 Leica TP1020 27 4 instrument Setup 4 2 Installation site requirements i Because of the quantity of solvents used for processing and the solvent concentrations that build up for the instrument versions without fume control system containment shields and activated carbon filter we recommend installation in the labora tory s fume hood 780 mm ben 20M mr r_ 28 Fig 12 A This instrument may not be operated in hazardous locations The installation site has to fulfill the following conditions Stable and plane installation surface Minimum installation surface dimensions 850 x 850 mm Stable ambient temperature of 5 C to 40 C Relative humidity 80 maximum Necessary conditions for electrical connection Grounded wall outlet near enough so it can be reached with the mains cable sup
62. ove the tissue basket 72 Fig 23 Instructions for Use V 2 7 RevA English 04 2011 10 Warning and Error Codes Troubleshooting Warning and Error Codes Troubleshooting continued Leica TP1020 Use caution when lowering the carousel Keep your fingers out of the space between the container lid and the upper rim of the con tainer When you release the crank handle the carousel will start to slowly de scend Manual relocation to the next station e Rotating the crank handle in the direction of the arrow lift the carousel upwards until there is a notable resistance to the rotating movement When you release the crank handle the carousel will be lowered into the next processing station Skipping stations To skip one or more processing stations hold on to the crank handle to avoid that the basket will actually be lowered into the station e Next lift the carousel again with the crank handle until you notice resis tance to the rotating movement e To lower the basket into the station release the crank handle When using the manual transporting option infiltration times for all stations have to be user controlled e Toresume processing once mains power has been restored switch on the mains switch After mains power is restored the software recognizes any manual station changes that have occurred during the power failure When mains power is back the program is resumed as ori
63. plied to gether with the instrument il If you wish to install the basket holder for a second tissue basket you should do that now For mounting instructions see Chapter 13 Optional Accessories Instructions for Use V 2 7 RevA English 04 2011 4 Instrument Setup 43 Electrical connection 4 3 1 Checking the voltage selector setting The voltage selector is located in a shell 3 above the mains switch 2 at the rear of the instrument The actual selected voltage can be seen in the window 1 e Check if the setting showing in the window 1 corresponds to the nominal voltage in your laboratory e Remove the adhesive tape If the setting is correct proceed at 4 3 3 If the setting does not correspond to the nominal voltage in your laboratory the voltage selector MUST be adjusted to match your laboratory s Fig 13 electrical setting prior to connecting the instru ment to the mains A Important The voltage selector is factory preset Before connecting the instrument to the mains make sure the correct setting matching your laboratory s power supply has been selected The socket for connecting the mains cable located at the rear of the instrument is sealed with an adhesive tape which indicates the factory preset voltage for your particu lar instrument Connecting the instrument to the mains with the voltage selector set at a wrong value can cause severe damage to
64. ptional accessory for instruments With fume Control SYSTEM iiii ii 87 Hiert inf rmati eieiei TA Accessories steet EES AppendiX aida i 15 1 Modifying the instrument configuration 15 2 Reagents appropriate for use with the instrument eececceeseeseeceesessessessessessesseeseeseeseeeeseeeteseeeeesaeens 92 15 4 UFactory Set programs rhelee bic een 93 Instructions for Use V 2 7 Rev A English 04 2011 1 Important Information Symbols used in this manual and their meaning Warnings appear in a gray box and are marked by a warning triangle A Notes i e important user information appear in a gray box and are marked by an in formation symbol i 5 Figures in brackets refer to item nos in drawings Function keys to be pressed on the instrument touch screen are written in bold print capital letters ENTER Qualification of personnel e The Leica TP1020 may be operated only by trained laboratory personnel e Alllaboratory personnel designated to operate the Leica instrument must read these Instructions for Use carefully and must be familiar with all techni cal features of the instrument before attempting to operate it Leica TP1020 Designated use The instrument has been designed so that it is safe to use by the operator as well as for professing speci mens provided that it is operated according to the present Instructions f
65. r carriers 2 Wax baths 1 8 liter 1 Standard tissue basket 1 Tubing band clamp 1 Activated carbon filter Standard 1 Activated carbon filter for formaldehyde 1 Exhaust hose diameter 50 mm 4 m long All instrument variants of the Leica TP1020 come with the following accessories 4 Replacement fuses T 10 0 A L250V 1 Setof power cables 1 Power cable for Germany 1 Power cable for USA Canada Japan 1 Power cable for UK ST BU F 5A 1 Screwdriver 5 5 x 200 1 Crank handle size 4 mounted 1 Instructions for Use for Leica TP1020 1491020 US02 14 0422 42545 14 0422 42545 14 0422 30665 14 0422 30585 14 0422 31973 14 0422 30673 14 0422 30674 14 0422 31975 14 0600 00759 14 0411 45754 14 0411 13558 140411 13559 14 0411 27822 14 0170 10702 14 0222 30663 14 0422 80001 o You will find the standard accessories and in case you ordered them further parts in the 1 upper portion of the packing box Compare the delivered parts with the packing list and your actual order If you find any discrepancies please contact your local Leica sales organization immediately Leica TP1020 17 3 Instrument Components and Specifications 3 4 General overview Leica TP1020 with additional wax bath optional accessory Carousel cover Reagent station lid Tissue basket holder Standard tissue basket Glass container with holder Wax bath
66. r to its upper end position clean the carousel axle with a cleaning cloth and subsequently apply a thin coat of machine oil Never attempt any repairs on the instrument of wax baths on your own by doing so you will loose any warranty claims 77 12 Maintenance 12 2 Wax bath 12 2 1 Altering the standard working temperature switch on when the actual temperature of the paraffin is 5 C below standard working temper ature When working with paraffin that has a melting point below 58 C the instrument working tem perature can be readjusted with the corresponding setting screw e Use a screw driver to turn the setting screw A Leica 1 to the desired value If you find that the paraffin does not melt com WAX BATH TP 1020 pletely after lowering the working temperature Se D422 30665 slightly readjust again 1 Factory set standard working temperature is 65 C The wax bath heating will automatically Leica Microsystems Nussloch GmbH Made in D 69226 Nussloch Germany Fig 24 78 Instructions for Use V 2 7 Rev A English 04 2011 12 Maintenance 12 2 2 Reset after excess temperature shutdown Leica TP1020 D If the actual temperature rises above the range of normal working temperature an excess temperature switch off mechanism re sponds The wax bath heating is disconnected The yellow signal lamp is extinguished Use of the wax bath can be resumed only af ter a cooling do
67. r to unload them for further special processing The pause period is terminated by pressing the PAUSE CONT button The processing cycle is resumed and the remaining immersion time for that station is completed without interruption STOP button To immediately stop the carousel up and down movement press once or to abort a processing cycle in progress press twice The display reads STOP 39 5 The User Interface 40 While the carousel is moving the dis play reads WAIT and two blinking arrows indicate the direction of the movement Only when the carousel has completely come to a halt this in dication will disappear from the dis play After the vacuum function is switched off using the V button rais ing the basket is delayed until the venting of the container is completed The Leica TP1020 is available with and without a vacuum function The instrument versions without vacuum functions do have the V button in the control panel however it is not func tional V is not displayed Of course a vacuum function cannot be activated with the V buttons in those cases Manual processing keys These buttons are only functional in the manual processing mode a while and while an automatic processing mode is paused via the PAUSE CONT button CIRCLE ARROW button to rotate the carousel To move the tissue basket to the next station Rotation is only clockwise and w
68. ram Otherwise select another program The cursor blinks at the program number data entry position For a program to be selectable for use at least one infiltration time for one processing station must have been set 1 AII corresponding parameters are displayed such as number of baskets starting position Lloi vac 1h00 W 05 e To start a program press START e Select the desired program number by pressing PLUS or MINUS e To start the selected program immediately press START again 56 Instructions for Use V 2 7 RevA English 04 2011 9 Operating the Instrument in the Automatic Processing Mode Immediate start continued e To start the selected program immediately press START again The warning codes W 04 W 06 see Chapter 13 are displayed for pro grams of less than 8 hours duration from start until the basket reaches the first wax bath Check if the available amount of time is sufficient for the paraffin to liquefy completely If this is not ensured fill the wax baths with liquid paraffin e To override the warning codes and start pro cessing press KEY and START simultaneously After the drain time of 60 seconds the carousel will automatically move to the programmed start ing position While the basket is being moved the display reads the numbers of the container stations the carousel is rotating past Instead of the infiltra tion time h will be displayed As soo
69. rocessing Mode 9 8 Removing the specimens 9 9 Finishing the daily routine i 66 Due to the relatively long time paraffin requires to melt the instrument should not be turned off routinely after finishing the day s last processing cycle Lift the carousel Allow for the tissue basket to drain in that position Lift the tissue basket slightly with your hand and pull it out of the basket holder in a hori zontal movement Lower the carousel Check the filling level and quality of the liquid in each station reagent and wax baths and refill or exchange completely if necessary Clean the edges of the reagent containers and wax baths as well as the seals of the lids refer to Chapter 14 Cleaning Wipe the control panel clean Press KEY to lock the panel and that way protect program settings against accidental alteration or misuse Instructions for Use V 2 7 RevA English 04 2011 10 Warning and Error Codes Troubleshooting 10 1 Warning codes LJo 4 W 03 Solid paraffin takes several hours to melt Please keep this in mind and make sure to switch on the instrument early enough so the paraffin will be molten and ready for use when necessary i OI LI Leica TP1020 The warning codes W 01 W 06 may be dis played W 01 W 02 W 03 Paraffin in wax bath station 10 11 12 is still solid W 04 W 05 W 06 Paraffin in wax bath station 10
70. ssories 13 3 Basket holder for second tissue basket o To increase the specimen throughput the instrument can be retrofitted with a basket hold 1 er for a second tissue basket A second tissue basket standard or three level type has to be ordered separately Standard delivery 1 basket holder for second tissue basket 1 wax bath 1 station holder for wax bath Fig 28 Leica TP1020 83 13 Optional Accessories Basket holder for second tissue basket continued 8a 8b 84 Fig 29 Installing the second basket holder Rotate the carousel cover counterclockwise until it can be removed from the axle 3 in an upward movement The second basket holder is installed at position 2 For this purpose the existing cover and bar must be removed Clutch the rod 5 with a pair of tongs and release the slotted screw 8a on the upper side of the disc 4 Insert the rod 5 of the second basket holder in the bore on the bottom of the disc 4 Adjust the holding device 6 for the second basket in the same way as the holding device in position 1 and hold it in that position Put the locking washer 7 around the bore in disc 4 Insert the Allen screw 8b into the bore from above and tighten with an Allen key no 3 Insert the hub 2 of the carousel cover 1 from above into the axle 3 of the center piece To secure hold on to the disc 4 and rotate the carousel cov
71. t menu items Signal level 1 4 3 Wax bath ProgPreset SystemReset Editing the configuration menu e Switch off the mains switch e Switch the mains switch back on While the display still reads TP1020 V x xx press PROG for a short while The first configuration menu item is displayed Modifying the configuration Signal level Setting the desired signal type and frequency Level 1 Audible signal in case of power failure and malfunctions Level 2 Level 1 audible signal for interruption warning codes and end of program Level 3 Level 2 audible signal for acknowledgment of warning codes and Error messages Level 4 Level 3 audible signal every time a button is pressed 89 15 Appendix Modifying the instrument configuration continued e For the next menu item press the CURSOR button e To change the setting press the PLUS MINUS keys Configuration 3 wax bath NE The next standard setting is displayed 3 Wax bath Is a third wax bath being used i In station no 10 you can install a third wax bath instead of the reagent station If you do so you have to modify the configuration menu accordingly otherwise the instrument will not function properly If you change from a three wax baths operation mode back to two wax baths you have to go back and readjust the configuration menu e To change the setting press the PLUS MINUS keys e For the next menu ite
72. trouble free operation of the instru ment make sure to comply with the following instructions and warnings Instructions for Use V 2 7 RevA English 04 2011 2 Safety Transport and installation A Keep in mind Chapter 3 Technical data During transport keep the instrument upright When transporting the instrument do not lift it up by the carousel cover This instrument may not be operated in hazardous locations Attention the voltage selector is factory preset Before connecting the instrument to the mains make sure the correct setting matching your laboratory s power supply has been selected The socket for connecting the mains cable located at the rear of the instrument is sealed with an adhesive tape which indicates the factory preset voltage for your particular instrument e Connecting the instrument to the mains with the voltage selector set at a wrong value can cause severe damage to the instrument e When adjusting the voltage selector setting the instrument must be disconnected from the power supply e The instrument may only be connected to the mains with the cable supplied together with the instrument and it may only be connected to grounded sockets Operating the instrument e The carousel may not be rotated manually Severe damage will result from doing so e Caution when lowering the carousel Keep your fingers out of the space between the container lid and the upper rim of the
73. trument can be rotated thus ensuring easy access to all stations at any time Leica TP1020 47 7 Preparations for Tissue Processing 7 1 Filling the reagent stations e Lift the carousel cover e Fill all stations with the corresponding reagents Make sure to observe the minimum and maximum level indication marks Spilled reagents have to be wiped away immediately In case of long term exposure the instrument surfaces are only conditionally resistant to solvents e Mount every container onto the station holder at the corresponding station o The container rims and sealing rings of the lids always have to be 1 clean The lids have to close tightly otherwise larger amounts of solvent fumes will escape and in instruments with vacuum func tion vacuum will not be generated 48 Instructions for Use V 2 7 Rev A English 04 2011 7 Preparations for Tissue Processing 7 2 Filling the wax baths The heated wax baths may only be used with paraffin Under no circumstances may they be filled with solvents When solvents heat a highly explosive mixture builds up Caution The interior containers of the wax baths become very hot when the heating function is activated Do not touch the gray up per rim of the containers with your hands Risk of injury Caution when handling hot paraffin Risk of injury Altering the standard working temperature special wax bath model
74. wn period For cooling down disconnect the wax bath connector plug from the socket at the rear of the instrument or switch off the main switch e Disconnect the wax bath connector plug from the socket at the rear of the instrument e Wait for the wax bath to cool A Empty the wax baths completely before the paraffin cools When cooling down the paraffin contracts which can cause dam age to the wax bath Operational test e Plug the wax bath back in e Check whether the yellow pilot lamp lights when heating is activated e Wait until the paraffin is completely molten The set standard working temperature has been reached when the pilot lamp goes out e Measure if the actual temperature of the paraffin corresponds to the standard working temperature If there is any doubt whether the wax bath is functioning trouble free it has to be exchanged 79 12 Maintenance 12 3 Replacing the fuses Burnt out fuses may only be replaced by fuses of the same type and specification See Chapter 3 Technical Data A Before exchanging the fuses switch off the mains switch and unplug the instrument Fig 25 The fuses are located in the voltage selector The currently selected voltage can be seen in the shell 3 small window 1 in the voltage selector shell e Remove the shell 3 as described in Chapter Insertthe voltage selector shell together with 4 3 2 A

F44 How to use Leica TP1020 Automatic Tissue Processor

Contents

Download Pdf Manuals

Related Search

Related Contents