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Leica ASP300 S

1. In the VIEW EDIT PROGRAMS screen Fig 43 highlight a program as similar as possible to the program you wish to create This minimizes the number of modification steps to be carried out Press COPY to copy the selected program The new program will have the same name as the program copied however the figure 2 will be added to indicate the change Highlight the line containing the new program Press PROGRAM NAME at the top of the table to display the keyboard Enter the new program name Editing program steps Press EDIT refer Fig 43 to access the PROGRAM STEPS screen In the headline you will find the PROGRAM NAME The colors on the left border of the table indicate the reagent groups to which the reagents belong The program steps are displayed in the order in which they are carried out For each program up to 13 steps can be defined The following characteristics of each program step can be edited Reagent name Duration of step with the exception of fill and drain steps Retort temperature if Ambient is selected the retort temperature indication remains empty Type of pressure and or vacuum cycle Retort drain time Delay step 43 5 Operation Editing program steps e To edita step highlight the corresponding line and press the respective headline e Inthe entry windows that pop up enter select the program step settings 3 High Priority Thursday 20 July 2006 1 00 55 PM a P 4 T
2. 22 4 3 Installing the accessories e Place the instrument in the definitive installa tion position Activated carbon filter e Unpack the activated carbon filter and insert it into the instrument see Fig 5 UPowsce nesrurencr Make sure to in sert the filter with the correct side facing upwards _LEICA 0476 34190 See label 29 at the front of the filter the arrow must point upwards Remote fill drain hose e Connectthe supplied remote fill drain hose to the remote drain connection on the front of the instrument refer Fig 6 e mportant When inserting the hose into the fill drain con nection item 18 in Fig 6 the connecting de vice 28 of the hose must lock with an audible click i If bulk containers can be located next to the instrument during remote filling and draining the hose may be shortened to improve handling and the fill drain rate If the hose is shortened a vee notch can be cut into the hose end to en sure a clear passage for fluid flow Operating Manual V 1 2 01 2008 4 Setting up the instrument Magnetic stirrer e Unpack the magnetic stirrer 30 Fig 7 and insert into the retort e Important Insert the magnetic stirrer so that the convex side 32 of the rotor blades 31 faces down Fig 7 Inserting the stirrer incorrectly will result in unsatisfactory stirring action Fig 7 4 4 Setting up the data connection
3. Cleaning solution bottles Maximum bottle volume Printer Pretest check Fluid level sensor Recirculation pump in out a Time before 1st cycle b Time between cycles System setup Password status Type of password Reagent Management Status Software access control lock mode Leica ASP300S max 300 cassettes 4 3 40 to 65 C ambient or 35 to 55 50 to 65 C 1K 90 sec 80 120 140 sec adjustable 70 kPa g 35 kPa g 70 kPa g 35 kPa g 10 3 5 0 Optional accessory ON OFF ON OFF ON OFF 16 min 20 min Supervisor User alphanumeric freely selectable ON OFF ON OFF 17 3 Instrument components and specifications 3 4 Technical Data continued Hardware and Software 18 Large color Liquid Crystal Display LCD and touchscreen User friendly intelligent software 3 5 floppy drive and printer port Alarm system with two remote alarm sockets Password protected instrument supervisor mode Multiple specimen protection system Capacities 15 programs that consist of up to 10 reagent and 3 paraffin wax steps e Time per program step 0 to 99 hours 59 minutes e Delay time max 7 days Up to 300 cassettes can be processed simultaneously Four user programmable retort clean programs Wax clean program 10 reagent bottles 3 wax baths 3 cleaning solution bottles 1 condensate bottle Reagent temperature adjustable from 35 C to 55 C or ambi
4. Fax 49 0 6224 143 200 Internet http www histo solutions de Leica ASP300S These are determined only by the contract provi sions agreed between ourselves and our cus tomers Leica reserves the right to change technical specifications as well as manufacturing process es without prior notice Only in this way is It pos sible to continuously improve the technology and manufacturing techniques used In our products This document is protected under copyright laws Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods including any electronic systems and media requires express prior permission in writing by Leica Biosystems Nussloch GmbH For the instrument serial number and year of manufacture please refer to the name plate at the back of the instrument Leica Biosystems Nussloch GmbH Table of Contents 1 important information 0 cesses seeeeeeeseceesesaeseseeseseeaseeeseseeaeeenaesesaeeesaesenaesesensenaeeeas 6 Z RNY E A E E E A E 7 2l Salet AS EU ONS cienne a a a n aa A oi 1 ZZ N a E E 1 2 3 Integrated safety devices acccecs ote cesar tavstianse res taaerideasein abuntanican eacstdecansenataph shane rsundarieanedatareaneeneaeaet 11 3 Instrument components and specifications s susnnsnnnsununnnnnnnsunnnnennnnnnnnnnnnnnennnnnnnnen
5. Operating Manual Leica ASP300S V1 2 English 01 2008 Always keep this manual together with the instrument Read this instruction manual carefully before working with the instrument Leica ASP300 S Advanced Smart Processor Automated vacuum tissue processor Seica MICROSYSTEMS NOTE The information numerical data notes and value judgments contained in this manual represent the current state of scientific knowledge and state of the art technology as we understand It following thorough investigation in this field We are under no obligation to update the present manual according to the latest technical devel opments nor to provide our customers with addi tional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case In particular no liability whatso ever is accepted for any financial loss or conse quential damage caused by or related to compli ance with statements or other information in this manual Statements drawings illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products Published by Leica Microsystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Telephone 49 0 6224 143 0
6. This alarm is also external to the Leica ASP300S lf installed it might typically be connected to a remote dialer that sends an automated phone message to the person responsible for after hours problems The remote alarm Is only generated when the in strument cannot continue with a Processing pro gram i i Note that if installed the remote alarm will still operate even if the local alarm is not installed Both local and remote alarm op tions are relays that are voltage isolated from the rest of the instru ment When an error condition occurs the relevant alarm circuit closes The remote alarm device connected to the instrument must be rated at less than 2 amp A maximum voltage of 30 V DC may be present Each alarm is connected to plug 34 as follows see Fig 12 Common terminal tip 36 connection inside Normally Open Contact First sleeve 35 connection outside Normally Closed Contact Second sleeve 33 Threaded connection 25 4 Setting up the instrument 4 7 Switching the instrument on A 26 Caution The instrument MUST be connected to an earthed mains power outlet socket It is recommended that the ASP300 S be plugged into a wall socket that has Ground Fault Circuit Interruption GFCI protection as an additional electrical safeguard e Connect the instrument to the mains power outlet socket If applicable switch the mains power
7. If the RMS is enabled the instrument will give you warning messages when reagents have been used for too long Warning messages for normal reagents are giv en based on up to 3 criteria warning messages for paraffin wax on up to 6 criteria The warning messages are displayed at the end of a cleaning cycle From there you can go di rectly to the REAGENT STATUS screen In addition warning messages on a yellow back ground are displayed in the program start screens Leica ASP300S In the reagent status screen Fig 64 you can display and update the actual reagent status of each station and you can set the stations as full or empty The following parameters are dis played e Age of reagent e Status of each reagent station e Warning thresholds that trigger warning messages red fields To change a reagent status highlight the corresponding line and press the desired key symbol In addition you can go directly to the REAGENT and STATIONS screens to make changes there Fig 69 Criteria for warning messages For normal reagents the following warning crite ria apply e number of blocks processed e number of programs carried out e number of days lapsed For paraffin wax three additional criteria can be applied e number of blocks processed e number of programs carried out e number of days lapsed since the wax has last been cleaned 59 5 Operation 5 5 System Monitor _ 7 The SYSTEM
8. gram itself is not deleted i The Favorite is only deleted from the FAVORITES list The pro 4 5 Operation 5 1 8 Editing the stations E The list below Fig 50 shows all stations allocated aa eug reagents currently defined in the instrument ViewlEdit Stations Thursday 20 July 2006 1 12 42 PM Battie 1 C CT M Fonnalin Fig 50 Adding changing reagents Allocating reagents Highlight the desired station either by pressing Only processing reagents except paraffin the reagent name or using the UP DOWN but can be allocated to stations 1 10 tons e Only a cleaning reagent can be allocated to e Press REAGENT NAME Station 11 e The SELECT REAGENT entry window pops up Only a cleaning alcohol can be allocated to a ert station 12 ae nee reagent at es e Only cleaning water detergent can be allocat patible with the respective station ed to stations 13 and 13 ext will be available for selection e Only paraffin wax can be allocated to the wax e Highlight the desired reagent and press OK to baths confirm 48 Operating Manual V 1 2 01 2008 5 Operation 5 1 9 Reagent groups E In this screen Fig 51 you find the colors used for each re gt Reagent Groups i agent group in the graphic program display Edit Reagent Groups T Cleaning alcohol 3 Cleaning waterdetengent amp o oe Ce Le
9. information on what has caused the error 61 6 Solutions 6 1 General The Leica ASP300S is equipped with a state of the art troubleshooting system lf an error is detected detailed error messages and corresponding user instructions on what to do are displayed on the touch screen For all errors the instrument will take corrective actions to protect the specimens unless an unsafe operating condition exists mh Details of all events are recorded in Run Log These events can be viewed by pressing MENU and then RUN LOG 6 2 Power failure Power failure during a processing program e lf a power failure occurs during a processing program the Leica ASP300S counts the time during as contributing to the step duration i e the step is not prolonged by the power failure e The Leica ASP300S does not however reduce the duration of subse quent steps should the power fail duration exceed the duration of the step in which the power failure occurred e fthe power failure has been long enough that the wax temperature may have dropped below a level suitable for using in the program the Leica ASP300 S will introduce a delay into the program before the wax step in order to ensure that the wax Is sufficiently melted Power failure during a paraffin or retort clean cycle e fa power failure occurs during a retort or paraffin clean cycle the total duration of the clean cycle will be extended by the power failure
10. press ALL PROGRAMS to access the All Pro grams screen where all existing processing programs are dis played All Programs e Press the symbol corresponding to the pro gram you wish to start e A graphic representation of the stations retort will be displayed The stations required for the program you are about to start will be colored in the corresponding reagent group colors Fig 63 e Unlike the Favorites regular programs can be modified before being started However any processing step modifications that are made only apply to the program that has just been selected 55 5 Operation 5 3 1 Editing a selected program Thursday 27 July 2008 1 32 11 PM a as Retort kPa ac Wian ere Level sensor Not full Wide Closed Fig 63 To modify a program before running It press EDIT A message will be displayed indicating that the changes will only affect the currently running program Dismiss the message with YES and the program editing screen will open Fig 64 a A a aime ies few tm _ emia few fw te eae fw sas e et pman f e e _ eq eer Pew e Fig 64 56 Redefine the program finish time e Press FINISH TIME In the entry window set a new finish time Finish Time Fig 65 e Enter the day of the week when you want the program to be finished e Enter the time in 24 hour clock when you want the program to be finished e Pre
11. when calculating the proper pressure or vacuum Fig 35 Updating software New versions of the ASP300S operating software are provided on diskette Such upgrades generally contain two to three diskettes Do not perform a software upgrade if you do not have all of the diskettes in the upgrade package Read the instructions in the upgrade pack with care as they may contain additional version specific information as well as new or modified procedures parameters and data that must be loaded or modified to ensure that the new software version functions correctly 1 Make a backup copy of the instrument s current data You will need an empty formatted diskette for this purpose Upgrade 2 Insert Disk 1 or similar of the software upgrade into the diskette drive Software 3 Press UPDATE SOFTWARE dismiss the safety queries with YES OK and follow the onscreen Instructions Insert Disk 2 into the diskette drive when prompted Continue with the remaining disks if any 4 When the update process Is complete the instrument s software will reboot the screen will switch off and restart as tt would when switch ing the instrument off and back on Leica ASP300S 37 5 Operation Configuring a printer gt _ eae On the INSTALLATION screen press PRINTER TYPE to feat display the list of the printers that can be connected e The Leica ASP300S works with any printers that are com Printerlype l 7 pat
12. 01 General enspe ai E E R 60 O2 FONETI IUTE e A A E 60 6o MOUDE NOO UNO eee A O TA 61 6 4 Typical fill or drain problemS ssesesssssssesrrsrsrurunurururururununununanununananannnnnnnnnnnnnnunununununnnununanananannnnnnnnnnnnnnnns 62 7 Cleaning and Maintenance cccssssssssssssessssesessseeseeaeseeseeaeseesesassnsaesaeseeaeseesesasseesnsas 63 L Cran pod aSa a A T E T 63 Tes Wel E AR EEEE E E E A E A E EEE EE AE O A E 63 L12 Wax ClO AN progi aI annonsar aare Ea aara A araa 65 ASRS Ma GE a eraa E E E EE 66 Te Generale caning SUC 0S enn A aA E E R 67 1 2 1 Daily cleaning and maintenance ssesssssssssrsrerururururunununununununananananunnnannnnnnrunununununununanananununannnnnnnnnrnnnnnns 69 1 2 2 Periodic cleaning and maintenance ssesssssessrsrsrsrururunurunununanananarannnannununnrununununununnnanananunnnannnnnnnnnnnnnnnns 71 71 3 Preventative maintenance schedule ccssceesscssssssscscssssrsseseseeseessncessesensescessrsensensassnsensasensenseneates 12 8 Warranty and Service sssusususususnsnnnnnnnunununununnnunnunununununununnunununununununnnnnnunununununnnnnnnnnnnnnn annann nae 74 Leica ASP300S 5 1 Important information Symbols used in this manual Warnings appear in a gray box and are marked by a warning triangle A Notes i e important user information ap pear in a gray box and are marked by an information symbol H Flammable solvents and reagents are marked with this sym
13. MONITOR screen supplies important infor System Monitor mation on the Leica ASP300 S system status PUMP AGE Indication of hours of service of the pressure vacuum pump A warning message Is displayed according to the warning threshold set After ap prox 1 000 hours of use have Leica Technical Service carry out a preventive maintenance of the pump CARBON FILTER AGE Indication of hours of service of the activated car bon filter A warning message is displayed accord ing to the warning threshold set The carbon filter must be exchanged after approx 3 months of use Fig 70 j After replacing the filter press RESET to reset the filter age to 0 ROTARY VALVE RETRIES Provides information on the rotary valve status If the figure indicated Is higher than 50 a preventive maintenance is recommended FILL DRAIN This screen displays the average cae fill drain times of all stations ae n seconds short term average duration of last 5 fills drains long term average duration of last 20 fills drains In general these values tend to differ slightly however there should not be any extreme differ ences If one of the figures displayed exceeds 250 call Leica Technical Service There might be an obstruction which could Cause an instrument malfunction Fig 71 60 Operating Manual V 1 2 01 2008 5 Operation 5 6 On line help Help index ASP300S help index Sen
14. Practically vibration free floor e Room temperature constantly between 10 C and 35 C e Relative humidity maximum 80 non con densing e Avoid vibrations direct sunlight and heavy variation in temperature Moving the instrument 42 Electrical connection A Attention Follow the instructions below care fully to avoid damage to the instru ment CAUTION Unit wired for 230 240 Vac Refer changing to qualified service personnel 20 Fig 3 The chemicals to be used in the Lei ca ASP300S are both flammable and noxious The Leica ASP300S must be set up in a well ventilated area free from any ignition sources Do not operate the instrument in rooms with explosion hazard A After the instrument has been unpacked see un packing instructions attached to outside of trans port crate it must be touched only atthe points marked with dots for transport to its final site of installation Once the instrument has been installed there the brakes on the instrument rollers 24 must be locked A The instrument must be installed such that the mains switch at the rear of the instrument item no 42 in Fig 4 can easily be accessed at any time e Check the voltage label Fig 3 on the rear of the instrument to ensure that the instrument delivered is set to the correct voltage range A Caution Severe damage may occur if the in strument is conne
15. and condensate bottle seal lubrication e To ensure easy removal of the reagent and condensate bottles lubricate the O ring seals on the plug in nozzles with the O ring lubricant supplied i This procedure is particularly important with bottles containing chloroform O rings that have not been lubricated swell when exposed to chloroform The reagent bottles can then become very difficult to remove Checking the retort lid seal e Regularly check the retort lid seal for damage If the seal is damaged it must replaced without delay 12 Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance 7 2 2 Periodic cleaning and maintenance Cleaning the reagent bottles e Empty and clean the reagent bottles using a bottle brush and a laboratory detergent in warm water Never clean reagent bottles in an automatic dishwasher The reagent bottles are NOT dishwasher proof e Refill and reconnect the bottles once cleaned Make sure the bottle lids are tight and the bottles are properly seated in their home position at the rear of the reagent cabinet The reagent bottles must be properly engaged in the home po sition in the connection manifolds at the rear inner wall of the reagent module Failure to correctly plug reagent bottles into the manifold will cause an interruption to the processing run and may result in spilling of reagents e While the reagent bottles are outside of the reagent
16. cabinet wipe the stainless steel inner walls of the reagent cabinet with a damp cloth moistened with a mild detergent For further periodic cleaning and maintenance steps refer to the mainte nance schedule on the following pages Leica ASP300S 73 7 Cleaning and Maintenance 7 3 Preventative maintenance schedule Maintenance activity daily weekly monthly every 3 months Lubricate reagent bottle 0 rings and check for damage x by using the re mote fill drain system Wipe dry retort and lid after completing retort clean cycle Clean exterior of instrument with soft cloth and minimal amount of xylene Check retort filter screen for tissue or wax residue Remove residual paraffin from inner surface of wax bath lid Inspect and if necessary clean retort lid seal Inspect and if necessary clean wax bath lid seal Inspect and if necessary adjust paraffin levels Inspect reagent container levels 74 Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance Maintenance activity daily weekly monthly every 3 months Inspect and if necessary clean wax bath strainers Inspect wax bath air vent and if necessary clear Inspect and empty condensate bottle Clear inlet holes if necessary Examine activated carbon filter Replace carbon filter Check fluid level sensors a
17. handling paraffin wax or removing baskets molten paraffin is hot and may cause burns Also avoid personal contact with paraffin stations and retort walls they can be very hot as well Dispose of waste solvents with care according to local regulations and the waste man agement policy of the company or institution Do not clean reagent bottles in an automatic dishwasher they are NOT dishwasher proof Warnings Cleaning and maintenance AN Prior to each maintenance and or cleaning switch the instrument off and disconnect mains power Do not clean the instrument with solvents containing acetone or xylene No liquid may be spilled into the internal components of the instrument neither during operation nor during cleaning When working with cleaning detergents comply with all safety instructions by the manu facturer of the product and the laboratory management policy The condensate bottle should be inspected at least once every week and if necessary emptied 10 Operating Manual V 1 2 01 2008 2 Safety 2 3 Integrated safety devices Overpressure protection Overcurrent protection Overheating protection Over vacuum protection Overcurrent protection Leica ASP300S In the case of power failures or other processing problems the Leica ASP300S incorporates specimen protection features such as a fluid level sensor and sophisticated software controls which ensure that processin
18. instrument parameters From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE to go to the MORE MENU FUNCTIONS screen Fig 21 Fig 22 In the window MORE MENU FUNCTIONS press SYSTEM SETUP Program Options Display Buzzer he ore The _ SYSTEM SETUP screen is divid Num Blocks PIeS ar i ed up into five selection fields Buzzer Volume oe instrument e Program Options fer 7 e Display Buzzer e Date Time e Security e Instrument Fig 23 Leica ASP300S 31 5 Operation Program Options Station Sequential i 1 Wax l 2 Wax ll 3 Waxill Prompt for Disabled Num Blocks l Fig 24 Wax Bath Order Date Time 20 0712 Time 10 07 18 Fig 25 32 PROGRAM OPTIONS Select the options with which programs are executed here Press a button to change the associated value e STATION ALLOCATION by Age or Sequential by Age RMS is activated reagents will automatically be used in order of increasing cleanliness Sequential reagents will be used in sequential order of stations e WAX BATH ORDER Auto or 1 2 3 Can only be activated when STATION ALLOCATION is set to Sequen tial otherwise the order of wax baths will be managed by the RMS e PROMPT FOR NUM BLOCKS Enabled or Disabled Enabled When starting a program the actual number of blocks must be entered This is recommended with the RMS being enabled Disabled Number of b
19. stop the instrument during operation Always press PAUSE before opening the retort lid during processing to allow the retort to vent Do not remove the wax drain hose or the remote fill drain hose until the fill or drain has been completed as pressurized air is used to clear the hose after each fill drain After refilling replacing the reagent bottles ensure that the lids are tight The bottles must be properly pushed home into the connection manifolds at the rear inner wall of the reagent module Failure to correctly plug the reagent bottles into the manifold will cause an interruption to the processing run and may result in spilling of reagents Fixatives containing mercuric salts acetic or picric acid will corrode metallic compo nents in the instrument After each paraffin step a retort clean cycle must be run They are also available on the Internet http www msdsonline com 1 Material safety data sheets can be obtained from the supplier of the chemicals Leica ASP300S 9 2 Safety Warnings handling reagents Be careful when handling solvents Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument Reagents used for tissue infiltration can be both toxic and or flammable To avoid damaging the instrument only use those reagents that are listed in chapter 3 5 Do not use acetone benzene or trichlorethane in the instrument Use caution when
20. time calculation In administrator mode open SERVICE FUNCTIONS Fig 59 Press TIME UNTIL WAX MELTED A safety query will be displayed Dismiss it with YES Enter the desired delay in the time screen and press OK Fig 60 correct lf the instrument attempts a paraffin step with the paraffin only partly molten severe instrument malfunctions may occur A Only change the melting time if you are absolutely certain that the new time entered is 54 Operating Manual V 1 2 01 2008 5 Operation 5 3 Running programs Programs can be started from two different screens FAVORITES Thursday 27 July 2006 7 51 28 AM Routine Overnight Small Biopsies Finish ASAP Routine Overnight RMS Finish ASAF Fig 61 In the ASP300 S you can define up to five Favorites A Favorite is a processing program that is fre quently used and therefore allocated to the FAVORITES list All settings are already programmed Only the number of blocks has to be entered if this func tion is enabled Starting a Favorite To start a Favorite press the corresponding sym bol on the Favorites screen the program will be started immediately After the program has been started its finish time and or other program parameters can still be changed as with any other program Leica ASP300 S ALL PROGRAMS Routine Ovemight eutine Bee aE Fig 62 In the Favorites screen
21. CK and REAGENT STATUS to access the reagent status table Fig 57 Highlightthe wax bath you justfilled and press SET AS FULL After about 90 minutes add another 0 5 kg of Wax pellets Do not insert the splash protector during the heating phase The pellets will take a total of approx 10 hours to melt affin level may NOT drop below the MIN level pos 2 Fig 58 Once the paraffin is completely molten rein sert the splash protector Software control Paraffin pellets have to be completely molten before the ASP300S allows any programs to be run The instrument software calculates when the wax will be ready for use and selects program starting times accordingly i e the paraffin will definitely be molten when itis required for the first paraffin step 53 5 Operation Adding molten paraffin To avoid damage to the thermal fuse molten wax that is added to the wax baths may have a maximum temperature of 70 C e If filling with molten wax do not fill above the MAX level see Fig 58 on the wall of the wax baths ae l e After a wax bath has been filled it must be The functions below should only be used by certified Service Staff Incorrect use of marked as full in the REAGENT STATUS these functions can cause the instrument to malfunction screen Printer Siaha idle Software control lf the wax baths are filled with mol ten wax itis possible to override the melting
22. Instruction for use for in vitro diagnostic instruments for professional use In addition the following in house standards were applied e DIN EN ISO 9001 2000 Leica Biosystems Nussloch GmbH 3 a Postfach 1120 HAAS CET MAAS OK D 69222 Nussloch Anne De Greef Safft March 10 2008 President Biosystems Devision Leica ASP300S 7 10 Information for the People s Republic of China ised by Electronic Products Bs atm Hazardous substances EZ ER wh TAL wb E me of the part DIRKA 2 RAR Ho Cd PBB PBDE i Ba AR E ited circuit boards mee mH ctronic components a ee ee chanical parts Bes ical components Lelee S gt les p X e ee a A RIKASASVREGMH PHS EEST 11363 2006 EMEA RES UT Indicates that the concentration of the hazardous substance in all materials in the parts is below the relevant threshold of the SJ T 11363 2006 standard RASA SURE EGREHR VY PHS SBMS 11363 2006 EME HN REEX Indicates that the concentration of the hazardous substance of at least one of all materials in the parts is above the relevant threshold of the SJ T 11363 2006 standard e The actual product may or may not include in all the part types listed above 78 Operating Manual V 1 2 01 2008
23. LATION menu installation Serlal umber SOO00000000 Copyright 1999 2004 VIL 10 1 55 Fig 32 Assigning an instrument name Instrument Name iii ty let flejlejlyty 02 02 0000 0 00B ISAE e eSLI LICI w COO e R a le aac Press INSTRUMENT NAME to display the key board Enter a name 20 characters maximum for the instrument Fig 33 The instrument name is also displayed on the FAVORITES screen 36 Use this menu to set the name of the instrument the language of the user interface and configure a connected printer The serial number of the instrument and the cur rent software version are entered at the factory and cannot be edited Selecting a language Press LANGUAGE to open the SELECT LANGUAGE menu Select the desired language there and press OK Fig 34 A message box will prompt you to restart the instrument in order to display the user interface in the selected language Press YES to restart the instrument and display the user interface in the new language Operating Manual V 1 2 01 2008 5 Operation Setting the altitude of the installation location in meters above sea level Site Elevation This is important as it affects the actual pressure in the retort Press SITE ELEVATION metres enter the value in the number field and press OK This parameter in meters must be entered to ensure that the ASP300 S makes the appropriate corrections
24. Manual V 1 2 01 2008 3 Instrument components and specifications Overview instrument components 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Basic instrument processor module Basic instrument reagent module Wax bath lids Retort lid Touch screen Cover flap of the instrument console Instrument console comprising Printer port Local alarm connection Remote alarm connection Floppy disk drive Serial port Activated carbon filter Condensate bottle Reagent bottle 13 units Drip tray Wax drain connection Remote drain connection Retort Wax baths Specimen basket Lid for specimen basket Splash protection Leica ASP300S Instrument components and accessories The processor module contains three paraffin wax baths a processing retort and also a touch screen with integrated key symbols as well as the electronic components The cassettes are held in three baskets 21 each of which has a capacity of up to 100 cassettes All processing occurs in the stainless steel retort under the pressure vacuum and temperature conditions selected The quick connect reagent bottles are stored in the reagent cabinet 13 3 Instrument components and specifications 3 2 Specific instrument options 14 Reagent Management System option RMS that displays the usage length and frequency of use of each reagent and also allows automatic orderi
25. Operation 5 2 2 Changing the wax Fig 55 Menu Station Drain Remote Fig 56 52 Draining the wax e Connectthe paraffin drain hose 36 to the wax drain connection 17 on the front of the instru ment Fig 55 and insert the loose end of the hose into a collecting container A Make sure that the hose is pushed all of the way onto the O rings of the wax drain connection While performing a paraffin drain ensure that the paraffin drain hose is securely placed in the remote container and is kept in place until the completion of the operation as the hose is automatically purged with air upon completion of the drain The hose should therefore not be re moved from the remote container until this cleaning step is complete In the FAVORITES screen press SMART SCREEN see previous chapter e Select the wax bath to be drained and the re mote container Remote Fig 56 Then press STATION DRAIN e Several safety queries will be displayed each of which has to be confirmed individually in order to continue Operating Manual V 1 2 01 2008 5 Operation Filling with wax pellets gt rans gt Fig 57 Fig 58 Leica ASP300 S Important During the melting process the par Fill the wax bath with wax pellets up to the max imum level line 1 in Fig 58 This requires ap prox 3 5 kg pellets per wax bath Leica pellets Press BA
26. T temp I Pw oa Reagent vaf fe enn ve tow 4 T 3 Ethanol Abaoluia x al Ethanol Absolute sco Fig 45 44 Operating Manual V 1 2 01 2008 5 Operation Editing program steps continued Setting a delay step A step that is nominated as a delay step will be extended to ensure that a program will finish at a specified time Finish Time To set a delay step e Highlight the program step you wish to define as delay step e Press DELAY The delay symbol is moved to the selected step thus defining the step as delay step Copying program steps e Highlight the step you wish to copy e Press COPY e f required modify properties of the step i Remember that a program step cannot be copied if the program already contains the maximum number of 13 steps e Use the MOVE UP MOVE DOWN buttons to move program steps up or down within an existing program without having to recreate those steps Deleting program steps To delete a step from a program e Highlight the step you wish to delete e Press DELETE Remember that it is not possible to delete a step from a program containing only one single step Programs must consist of at least one step Leica ASP300S 45 5 Operation 5 1 7 Favorites In the ASP300S you can define up to five Favorites Favorites can be programmed to end either e as soon as possible ASAP or e ata pr
27. The MORE MENU FUNCTIONS screen will appear Press REAGENTS e The SET UP REAGENTS AND WARNING THRESHOLDS screen opens e To add a reagent e Press INSERT to display the keyboard e Enter the new reagent name e Press OK to confirm e You will then automatically be prompted to select the reagent group Thresholds Monday 31 July 2006 3 05 57 PM Select the reagent group tere Clean Cina Fig 39 Allocate the new reagent to the desired group and press OK to confirm Fig 38 Leica ASP300S 39 5 Operation A Allocation of reagents to the correct reagent group is the basis for compatibility monitoring Allocation to the wrong reagent group can lead to reagent cross contamination Changeable parameters Fig 41 40 Entering modifying reagent thresholds If warning thresholds are required for a certain reagent enter them as follows i The respective threshold value applies for all reagent stations Highlight the reagent to be modified either by pressing the reagent name or using the UP DOWN buttons Press the header of the parameter to be changed the corresponding entry screen opens Enter a new threshold or if no warning Is desired press CLEAR to remove the threshold altogether Press OK to confirm containing the same reagent Changing reagent names or reagent groups i If a reagent is already used in a program it can neither be renamed nor
28. around the retort lid seal with the plastic scraper provided Thoroughly remove all wax deposits from around the lid seal e For convenience the lid may be removed during cleaning Lift the lid to a vertical position release the hinge lock and pull the lid toward you Only use the plastic scraper provided when cleaning the retort lid and seal to avoid damage to the retort lid seal and to the PTFE coating on the retort lid Do not damage the edges of the seal with the scraper Cleaning the retort e The retort may be wiped clean using a cloth moistened with solvent xy lene or alcohol or mild detergent Ensure that the air holes at the top front of the retort are open Cleaning the retort strainer e Use alcohol or xylene to clean the strainer located at the bottom of the retort For convenience the strainer may be taken out to remove all solid dirt Cleaning the touch screen e Qn the MENU FUNCTIONS screen press CLEAN TOUCH SCREEN e Clean the touchscreen e Press ENABLE EXIT to enable the BACK key symbol Press BACK to re turn to MENU FUNCTIONS Leica ASP300S 71 7 Cleaning and Maintenance Cleaning the processor module top surface To ensure proper sealing of the lids at all times it is important that you e remove both lids for cleaning e first scrape the stainless steel surface with the plastic scraper and then wipe down to remove all solid matter around the retort and paraffin wax Stations Reagent
29. ax Clean ll Last cleaned 180401 Wax Clean I Last cleaned 260401 Fig 74 Leica ASP300S Fig 75 The Leica ASP300 S contains a wax clean feature that removes xylene from used wax On the CLEAN PROGRAMS screen select the wax bath symbol corresponding to the wax bath the content of which you wish to clean In the event that the last reagent in the retort was not compatible with paraffin run a retort clean before starting the paraffin clean A paraffin clean consists of the following steps e The paraffin is transferred into the retort e n the retort the paraffin is heated to the maximum temperature e The retort is evacuated to draw off the xylene vapor The duration of the wax clean program Is pro grammable For that purpose e Qn the View Edit Programs screen highlight the wax clean program and press EDIT e Enter the desired duration We recommend a duration of one hour e Press OK to confirm 67 7 Cleaning and Maintenance 7 1 3 Smart Clean The smart clean prepares the ASP300S for transport During a smart clean a series of air cleaning steps will be applied which will remove any excess liquid condensate from all internal tubing Prior to starting a smart clean a complete retort clean program must be run To start a smart clean press the SMART CLEAN button on the MORE MENU FUNCTIONS screen and press OK to confirm The smart clean itself takes approx 15 minutes and consi
30. bol Instrument surfaces which become hot during operation are marked with this symbol Avoid direct contact with these sur faces they may cause burns Figures in brackets refer to item 5 nos in drawings Function keys to be pressed on the instrument touch screen are written ENTER in bold print capital letters Qualification of personnel e The Leica ASP300 S may be operated only by trained laboratory personnel e The instrument may be operated only accord ing to the instructions contained in this manual Designated use The instrument has been designed so that it is safe to use by the operator as well as for pro cessing specimens provided that it is operated according to the present instruction manual The Leica ASP300S8 is a modular automated tissue processor designed for the following labo ratory applications e fixation e dehydration e paraffin wax infiltration of histological tissue specimens The Leica ASP300 S must be operated exclusively with the reagents listed in chapter 3 5 Compatible reagents A Any other use of the instrument is considered improper Failure to adhere to these instruc tions may result in an accident personal injury damage to the instrument or accessory equipment Instrument type All information provided in this manual applies only to the instrument type indicated on the title page A name plate indica
31. can it be allocated to another reagent group The corresponding symbols will be disabled i e they will not be surrounded by a blue border If a reagent is renamed all stations and programs linked to that reagent need to be reedited as well Highlight the reagent the name or group of which you wish to change Press the corresponding headline key symbol In the entry window or via the keyboard enter the new reagent group allocation the new reagent name Press OK to save the new reagent group reagent name Operating Manual V 1 2 01 2008 5 Operation Deleting reagents e Highlight the reagent to be deleted in the SET UP REAGENTS AND WARNING THRESHOLDS screen e Press DELETE e Press OK in the screen to confirm the reagent is deleted program cannot be deleted i Please remember that a reagent which is already used in a Fig 42 Adding new reagents to stations Go to SMART FUNCTIONS to remote fill the reagent bottle from an external bulk container or fill the reagent bottle manually After filling a bottle manually the bottle must be defined as full For that purpose e Press MENU to access the MENU FUNCTIONS screen e Press REAGENT STATUS e Inthe reagent list highlight the station reagent that has been filled manually e Press SET AS FULL to mark the station as full Upon activation of the RMS all warning thresholds for the se lected reagent are automatically re
32. chnical Data Nominal voltage Nominal frequency Main fuses Nominal power Dimensions W x D x H in mm Dry weight unpacked Weight packed Operating temperature range Relative humidity IEC 1010 classifications Altitude Local remote alarm relays Two factory preset voltages not user adjustable 100 to 120V or 230 to 240 V 50 to 60 Hz Two fast blowing melting fuses 20x5 mm UL approved e for 100 to 120V F 10 A 250 VAC e or 230 to 240V F 5A 250 VAC 1000 VA 595 x 680 x 1 325 approx 160 kg 220 kg 15 C to 35 C 10 to 80 non condensing Protective class 1 Pollution degree 2 Overvoltage installation category II e 800 V impulse 120 V systems e 1500 V impulse 240 V systems 2500 m maximum 30 V DC maximum 2 A 2 connections Both single voltage free changeover contacts both Normally Open and Normally Closed connections available Paraffin wax baths Number of baths Volume liters Melt time Temperature Temperature accuracy 16 3 4 3 per bath approx 10 hrs 40 to 65 C 1K Operating Manual V 1 2 01 2008 Instrument components and specifications Retort Capacity Reagent volume Temperature paraffin Temperature processing reagents Temperature cleaning reagents Temperature accuracy Filling time approx Drain time approx Impregnation vacuum Impregnation pressure Fill vacuum Drain pressure General Reagent bottles
33. cloth moist ened with mild detergent and dry Do not use solvents on painted surfaces and or the touch screen Checking the drip tray Regularly check the drip tray 16 for spilled reagents Fig 76 Fig 76 Leica ASP300S 69 7 Cleaning and Maintenance Draining the condensate bottle Exchanging the activated carbon filter Fig 78 Filter label T U Pcuance THIS FILTER ON LEICA PART NO 0476 34150 70 e Remove and empty the blue condensate bot tle and reinsert Fig 77 Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution e The life of the carbon filter cartridge 13 will depend on the reagent types used and the frequency of vacuum cycles which expel air from the instrument through the filter e n general carbon filter cartridges should be replaced after approx 3 months Fig 78 After replacing the filter press open the menu SMART MONITOR to reset the filter age to 0 See also chapter 5 5 e Prior to changing the filter we recommend a smart clean When inserting the new carbon fil ter make sure the arrow on the fil ter label points up Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance 7 2 1 Daily cleaning and maintenance Cleaning the retort lid e Remove wax from the inside of the retort lid and from
34. cted to a power supply voltage other than that to which it was originally set The power supply voltage for the in strument is factory preset and CAN NOT be altered by the user Operating Manual V 1 2 01 2008 4 Setting up the instrument Connecting the instrument to mains The instrument MUST be connected Once the instrument has been AN to an earthed mains power outlet switched on the mains switch ON socket OFF 41 should always remain in It is recommended that the ASP300 S position ON be plugged into a wall socket that has Ground Fault Circuit Interruption GFCI protection as an additional Important electrical safeguard Specification for connection 40 is Make sure to use the appropriate as follows mains cable for the local voltage 100 120 V or 230 240 V supply wall outlet max 200VA e Connect the mains cable 37 to the mains power supply socket 41 e The jumper cable 38 which is supplied as part of standard delivery connects the mains power supply output 43 to the electronics module input 40 Left Electrical connections at the rear panel of the instrument a a q Right f g Pi i Correct n a connection S of the z E cables 2026666606608 ss ss ss For Sakaleslim nnn A Any Ue aa lT Leica ASP300S 21 4 Setting up the instrument 13 18 Fig 5 Fig 6
35. e surrounded by a black border instead of a blue one Disabled Default state of the instrument All instrument and software functions of the ASP300S are fully accessible to all staff 34 Operating Manual V 1 2 01 2008 5 Operation LOCK MODE Enabled or Disabled Wen Functions i naan uy 06 OS PM The Leica ASP300S provides a feature called Maintenance View Edit Other Operations Lock to prevent access to any operations of the Leica ASP300 S to unauthorized users Enabled nabled The LOCK button is enabled in the menu func tions To enable a password has to be entered When activated LOCK prevents any user input until after a password has been entered Disabled All functions can be accessed at any time until the screen saver turns off the screen Fig 30 Entry keyboard The keyboard is displayed any time text needs to be entered e The keyboard headline 1 tells you what kind of text to enter e 30 characters can be entered into each entry field though sometimes not all characters entered can be displayed Important keys Shift 771 1 To shift to upper case symbols EGE GCgeac eoo0 jcis AltGr l w iS P a y hu 3 A F Permits the entry of special char i ters geo ogooogoooo0aag i B Ea a a 5 m m 8 a a a Deletes the previous character ES ce O Delete Deletes the entire line oc om Coe Fig 31 Leica ASP300S 35 5 Operation 5 1 3 INSTAL
36. ed Fig 68 Fig 67 e Press START to start the program Press OK to confirm the query that follows The NUMBER OF BLOCKS must be entered if RMS has been enabled 03 15 e The FINISH TIME button shows the time when the current program will be finished If anything occurs that delays the finish time the finish time 8 Jul indication will be updated accordingly Finsh Time e Before a program in progress can be modified the PAUSE symbol must be pressed After pressing PAUSE all buttons surrounded by a blue bor der can be accessed edited To modify or delete program steps press EDIT and in the entry window carry out any changes you wish to make Press CONTINUE to restart the program The finish time of a program in progress can only be changed as long as the delay step has not yet been completed e Press TABLE to change from graphic to tabular program display press GRAPHICAL to go back to graphic display 58 Operating Manual V 1 2 01 2008 5 Operation 5 4 Reagent status Reagent Status Monday 31 July 2006 1 26 28 PM Blocks Cycles Days Since Since Since Changed Changed Changed Cleaned Cleaned Cleaned 1 Formalin 500 J 5 Ful e e a A E T T E C E a0 E a r fe ehae ateate of 0 Tr eee ateoute 0 0 fs etnet vente C T 2 0 a want n 600 nn soo a Fn waxy tistowax soo 2 soo 2 run wana eitowax soo 3 ws soo a e Reagent warnings
37. edetermined finish time View Edit Favorites CA 20 Ea 2006 9 35 45 AM Gi 4 Adding and or modifying favorites e Highlight the program line to be altered J e Press PROGRAM NAME e Selectthe desired program and press OK to confirm Setting a finish time e Press FINISH TIME e Enter the finish time 24 hour clock Select ASAP if the program finish time is not to be delayed e Press OK to confirm If a preprogrammed finish time is assigned to a favorite a de lay will automatically be inserted into the program at the nomi nated Delay Step to ensure that the program finishes at the preprogrammed time 46 Operating Manual V 1 2 01 2008 5 Operation Delete Leica ASP300S Assigning a finish day In addition to the finish time you can also define a specific day of the week when a Favorite Is to finish e Press DAY e Select the desired week day in the list and press OK to confirm Assigning a symbol to a Favorite Assigning a specific symbol to a favorite makes it easy to recognize at any time e Press ICON in the table header e Select the desired symbol and press OK to confirm The first symbol in the list is empty select this option if you do not want to assign a symbol to a specific program Deleting a Favorite e Highlight the Favorite you wish to delete e Press DELETE The Favorite is deleted without any further queries
38. ent Paraffin temperature adjustable from 40 C to 65 C Choice of three retort drain rates of 80 120 and 140 seconds Up to 100 reagent names in memory Operating Manual V 1 2 01 2008 3 Instrument components and specifications 3 5 Compatible reagents The following reagents may be used in the Leica ASP300S Leica ASP300 S Fixatives 1 Formalin buffered or unbuffered 2 Formalin saline Dehydration 1 Ethanol 4 Butanol 2 lsopropanol 5 Industrial Methylated Spirits 3 Methanol Clearing 1 Aylene _ Prior to using these reagents contact your 2 Toluol P Leica representative or Leica directly for in 3 Chloroform formation on necessary protective measures Paraffin wax 1 Paraffin Caution Reagents other than those listed here may damage some com ponents of the instrument Do not use acetone benzene or trichlorethane in the instru ment Fixatives containing mercuric salts acetic or picric acid will i corrode metallic components in the instrument and shorten instrument life If you choose to work with such fixatives it is essential to perform a clean cycle which contains multiple water rinses each time after use to minimize damage In addition we recommend frequent and regular preventive maintenance by the Leica Technical Service 19 4 Setting up the instrument 4 1 Site requirements e The instrument needs approx 650 x 700 mm floor space e
39. ent sta tus all lists will be empty 6 Store actual instrument status on floppy disk 7 Display run log 8 Restart instrument Leica ASP300S Fig 16 System Diagnostics Menu If during initialization Touch here refer Fig 13 is touched and subse quently the supervisor password Is entered the SYSTEM DIAGNOSTICS menu Fig 15 opens up giving ac cess to standard instrument settings Attention These settings must be modified by experienced operators only Seri ous instrument malfunctions may occur if any settings are modified wrongly Fig 15 To exit this menu the instrument has to be re started press RESTART APPLICATION For instrument reinitialization press YES to con firm the query ARE YOU SURE YOU WANT TO Fig 16 i e Initialization starts again from the screen shown in Fig 13 Such prompts are issued before all important irreversible steps This allows the operator to undo changes caused by accidental key strokes 2 4 Setting up the instrument 48 Touchscreen functions Enabled 28 The Leica ASP300S is programmed and operated via a colored LCD touch screen The control software contains an on line help feature where detailed information on the operation of each screen on error messages and on the software functions can be found Help can be accessed from any screen by pressing the HELP button Fig 17 Button symbol
40. g can be completed successfully without damage to the tissue specimens e When power is off the air pump and air valves default to a safe condition retort vented no pressure generation e f the microprocessor control fails to stop the air pump at the right moment during retort pressurizing a separate electronic hardware circuit will cut pump power e In addition there is a safety relief valve that vents all excess air pump output to atmosphere e Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses An error is indicated and all heating is stopped by the microprocessor control if the instrument detects any of the following conditions e abnormally high temperature gt 75 C e contradictory results of the temperature sensors e failure of one or more heating power control components e lf the microprocessor fails to interrupt heating power independent temperature limiting hardware circuits limit the temperature rise to a safe level e fthe temperature limiting circuits malfunction an independent hard ware thermal fuse circuit cuts power to the heating elements e The vacuum system Is not capable of generating a dangerous vacuum condition e Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses 11 3 Instrument components and specifications 3 1 Overview instrument components 15 Fig 2 12 Operating
41. hey do not touch or be come caught on the retort wall Changing the clip e Once the clip is installed the handle 4 of the spec imen basket cannot be removed as otherwise the clip will be bent e Toremove or replace a clip all parts that are inside the basket e g spiral insert and separating walls must be removed from the basket e Then insert a suitable tool screwdriver 5 Fig 11 and pry the clip out from the inside AN Once bent clips may no longer be used and must be disposed of In this case in stall a new clip from the standard scope of delivery 24 Operating Manual V 1 2 01 2008 4 Setting up the instrument 46 Alarm functions The Leica ASP300 S is equipped with 3 different alarm functions Instrument alarm This alarm is generated from within the Leica ASP300 The Instrument alarm is used for all alarm messages Local alarm This alarm is external to the Leica ASP300 S e g in the office of the instrument operator The Local alarm is used when the Instrument cannot continue with the current program or op eration because of a problem Alarm connections optional lf required connect the local and or remote alarm systems to the alarm sockets 9 10 in Fig 8 at the instrument console using the phono jack 34 supplied 6 3 mm socket 9 Remote alarm socket 10 Local alarm Leica ASP300S Remote alarm
42. ible with commercial PC s e With a Centronics standard cable connect the printer to the printer port See chapter 4 4 Fig 8 e Inthe SELECT THE PRINTER TYPE window highlight the print er that is connected to the instrument and press OK If the printer driver you connected is not listed try a similar printer of the same manufacturer If this approach does not work call Leica Techni cal Service to have the required printer driver in stalled Fig 37 Printing run logs Printing is possible when the PRINT symbol is displayed Print For the PRINT symbol to be displayed a printer must have been configured for the instrument The following lists and protocols can be printed e reagent list e stations list e reagent status list e all programs e runlog e error log Installing custom help See chapter 5 6 Online help on page 61 38 Operating Manual V 1 2 01 2008 5 Operation 5 1 4 Editing the reagent list Adding new reagents 1 Enter the reagent name and allocate the new reagent to the reagent group It belongs to 2 Define the stations bottles that will be filled with the new reagent 3 Fill the stations bottles according to the reagent list Enter reagent names Enter reagent names via the REAGENTS screen You must be logged on at supervisor access level to proceed e From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE e
43. ica ASP300 S Fig 51 Selecting a new color for a reagent group Highlight the line containing the reagent color you wish to change Press COLOR in the table header the entry window SELECT COLOR pops up Select the desired color and press OK to confirm If you want to close the entry field without changing the color press CANCEL The new color will be used in all stations to symbolize the reagent group ithas been allocated to 49 5 Operation 5 2 Reagent handling 5 2 1 Fill drain reagents other than wax 1 Manually changing the reagents e Remove the appropriate reagent bottle from the reagent cabinet remove the bottle screw cap 4 e Drain refill the reagent by pouring to from a bulk container Use the in cluded funnel for clean filling i e On the REAGENT STATUS screen verify correct allocation and set the reagent bottle as full 100 Set as Full 2 Use the Remote Fill Drain features e Connectthe supplied remote fill drain hose to the remote drain connection 7 on the front of the instrument refer Fig 6 Chapter 4 3 page 22 Important Ensure that the hose is pushed all of the way onto the connection until a clear click is heard e Putthe loose end of the remote fill drain hose into the desired container for the drain fill operation as desired Fig 52 While performing a reagent fill drain ensure that the remote fill drain hose is secure
44. ill the reagent stations bottles either via re mote drain fill function SMART SCREEN or manually REAGENT STATUS Create the programs you need Both processing and retort cleaning programs can be copied and modified Allocate the most popular programs to the FAVORITES menu and if desired set a finish time for these programs and allocate a symbol 29 4 Setting up the instrument 4 10 Switching off the instrument 30 Exit Application lf the instrument must be completely switched off or disconnected from the mains please proceed as follows e Press MENU to go to the MENU FUNCTIONS screen e there press the button EXIT APPLICATION A message appears stating that all data will be saved and the system will now be shut down Shutdown in Progress Please wait while the system writes unsaved data to the disk Fig 19 When all data has been saved the following message appears Shutdown Computer Fig 20 The instrument can now be switched off by means of the ON STOP switch on the right of the instrument and by means of the ON OFF switch on the back of the instrument item 42 in fig 4 Press the RESTART button to restart the instrument Caution The Multistainer must only be completely switched off in this way Otherwise this can result in serious damage to the instru ment s hardware and data loss Operating Manual V 1 2 01 2008 5 Operation 5 1 Setting up the
45. ing up the instrument ASP300S first steps Access control Access levels user profiles in the ASPIO0S Meat is lock moder Preventing unauthorized acces no i Alarm functions The various alarm types and their functions Cleaning the ASP3008 The Leica ASP300 S contains an on line help feature accessible from any of the principal screens For information on basic problems refer to the Help Index which can be accessed by pressing INDEX on the Help screen Wednesday 2 August 2006 9 44 47 AM The index lists all help topics in hyperlink format Use the UP DOWN arrows to scroll s Press the PREVIOUS TOPIC button 1 to return from a seers specific topic to the index Press BACK 2 to go back to the screen from a where you accessed Help Cleaning the a Wihhout accidentally actvanig a oo a 7 _ Leica ASP300 S The CUSTOM HELP button 3 will only be displayed if user specific help files have been Fig 72 installed Detailed information on how to create and install customer specific help files can be found in the What is Custom Help help text i In addition to the Help Index there are context specific help texts These texts are displayed only when Help is accessed from a screen where a specific problem has occurred Accessing Help in case of error messages To access Help when an error message Is displayed e press DETAILS or e press the HELP button on the error message to obtain
46. ive table header To disable a step set the number of cycles to 0 Only cleaning water steps can be disabled The following characteristics of each step can be edited i The finish times of clean programs cannot be edited Program Reagent name only those reagents that are compatible with the reagent station being edited will be displayed Number of cycles the duration displayed is calculated based on the number of cycles Retort temperature temperatures from 50 to 65 C can be set duration is calculated automatically based on the number of cycles set Station 13 ext i Only reagents belonging to the cleaning water group can be al The term 13 ext stands for an additional station which can be used instead of station 13 Station 13 always contains cleaning water Some applications require the use of a cleaning water detergent mixture between the cleaning alcohol and the cleaning water step lf a program contains a 13 ext step the operator is prompted to insert bottle 13 ext when needed and to remove the bottle as soon as the step has been completed located to station 13 ext Station 13 ext may be used for clean programs only Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance 7 1 2 Wax clean program henu J Clean Programs Wax Clean Ill Last cleaned 1804 01 Rapid Claaning program Standard Cleaning Program Extended Cleaning program W
47. locks can be entered optionally not mandatory to enter no of blocks DATE TIME Be sure to verify that the date and time entered do actually correspond to the local time date as this will ensure that all programs are carried out correctly Press the buttons to access the entry windows where settings or correc tlons can be made if necessary Date and time setup screen Thu 20 Jul 2006 Gi d Ge rom me oe Operating Manual V 1 2 01 2008 5 Operation Display Buzzer DISPLAY BUZZER LCD Backlight e The number next to DISPLAY OFF indicates the remaining time in min Time mins aa Buzzer Volume Instrument until the screen saver after the last user action is activated e The BUZZER VOLUME can be set between 1 and 10 mans INSTRUMENT 60 Press WAX BATH SET TEMP to open the input screen Fig 28 for the wax Lomp bath temperature Set the temperature according to the value required by the wax used Select the highest permissible temperature for the wax to ensure that a min Wax temperature and run options screen Soo ccs Leica ASP300S imum loss of temperature occurs when filling the retort Access the RUN OPTIONS function to display the op tions selected for the cur rent program The options selected will be applied to ALL programs A printer option will be dis played only if a printer has been configured for the in strument The i
48. ly placed in the remote container and is not removed from the container until the operation is fully com pleted as pressurized air is used to clear the hose after each fill drain The hose should therefore not be removed from the bulk con tainer until this cleaning step is complete 50 Operating Manual V 1 2 01 2008 5 Operation Fill drain reagents In FAVORITES press MENU In MENU FUNC TIONS press SMART SCREEN The SMART SCREEN Fig 53 pops up The smart screen Is the initial screen for manual operation of the instrument The following steps should only be carried out by trained laboratory personnel experienced in handling reagents Status bar The following values are displayed e Retort temperature and pressure e Wax bath temperature e Value of the retort level sensor e Retort lid open or closed Status bar displayed in administrator mode Fig 53 Select station Press key symbol Remote drain e Qn the screen select reagent bottle no 3 and the bulk container Remote Then press STATION DRAIN e Make sure no reagent has been spilled Remote station Drain Remote fill e QOnthe screen select an empty reagent bottle no 3 and the bulk container Remote Press STATION REFILL e The remote fill procedure should be complet ed in less than 170 seconds Station Refill e Make sure no reagent has been spilled Fig 54 Leica ASP300S 51 5
49. n upright position The instrument MUST be connected to an earthed mains power outlet socket The instru ment must not be connected to an extension cord without protective earth conductor Ensure uniform current supply according the to set voltage The set voltage CANNOT be changed by the user Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set The instrument must be set up in a well ventilated area free from any ignition sources The chemicals to be used in the Leica ASP300 S are both flammable and noxious Do not operate the instrument in rooms with explosion hazard If there is a significant difference in temperature between the warehousing and the in stallation site of the instrument and if at the same time there is a high air humidity level condensation water may form In this case a waiting period of at least two hours must be observed before the instrument is switched on Failure to adhere to this waiting period may result in damage to the instrument Failure to adhere to this waiting period may re sult in damage to the instrument 8 Operating Manual V 1 2 01 2008 2 Safety Warnings Operating the instrument The Leica ASP300S may only be operated by trained laboratory personnel according to its designated use and per the present instruction manual The ON STOP switch at the side of the instrument may be used in an emergency to
50. nce with the local laws 76 Operating Manual V 1 2 01 2008 9 EC Declaration of Conformity CE EC Declaration of Conformity feica MICROSYSTEMS We herewith declare in exclusive responsibility that the Leica ASP300 S Vacuum Tissue Processor was developed designed and manufactured to conform with the e Directive 2006 95 EC of the European Parliament and of the Council Low Voltage e Directive 2004 108 EC of the European Parliament and of the Council electromagnetic compatibility and e Direktive 98 79 EC of the European Parliament and of the Council in vitro diagnostic medical devices The following harmonized standards were applied e EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements e EN 61010 2 010 2003 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 Particular requirements for laboratory equipment for the heating of materials e EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements e DIN EN 61010 2 101 2002 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 Particular requirements for laboratory equipment for the heating of materials e EN 14971 2007 Medical devices Application of risk management to medical devices e EN 591 2001
51. nd clean if necessary Inspect electrical connectors at the rear panel of the instrument Inspect and if necessary clean inside reagent bottles Check that the bottle caps and seals of the reagent bottles are in place and tight Check that the reagent bottles are fully inserted in the connection manifolds Leica ASP300S 15 8 Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the delivered contract product has been subjected to comprehensive quality control measures in accordance with Leica s internal testing standards The product does not have any defects and meets all stipulated technical specifications and or has all agreed properties The scope of warranty depends on the contents of the individual contract concluded The terms of warranty of the Leica sales organization or other organization from which the contract product was purchased shall apply solely Service information If you require technical service or need replacement parts please contact your Leica sales representative or dealer who sold this product Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Delivery date of the instrument Shutdown and disposal of the instrument The instrument of parts of the instrument must be disposed of in complia
52. ndividual options are described in chapter 5 3 1 33 5 Operation Security SECURITY Access rights to the instrument are managed here as Supervisor lt i Enabled Bete user profiles i Administrator mode is disabled on delivery Lock Mode f Enabled Set Pwd Fig 29 SUPERVISOR PASSWORD Enabled or Disabled Enabled Two different types of user profiles are available A password is required for supervisor level access to the in strument Enabling SUPERVISOR MODE already requires entering the password 5 1 2 Access levels The Leica ASP300 S may be configured to allow two levels of user access Operator symbol Operator access level OPERATOR symbol is displayed in the upper right corner of the touch g2 e Operators may run programs and view results On this level the screen all enabled buttons are surrounded by a black border Supervisor symbol Supervisor access level e Supervisors may perform all Operator functions and additionally create programs and perform the instrument set up functions At the Supervisor access level a status line is added to the SMART SCREEN functions containing information on the retort and wax baths see Fig 53 page 51 To access Supervisor level press SUPERVISOR enter the required pass word and confirm When entering the password the SUPERVISOR sym Supervisor Operator bol will be displayed instead of the OPERATOR symbol and all enabled buttons will b
53. ng of reagent sequence thus eliminating the need to physically move reagent bottles After renewal of one or more reagents in a se quence the RMS automatically uses the reagents in order of increasing cleanliness In process fluid recirculation tidal agitation for continuous and efficient mixing of fluids Remote fill drain system can drain and refill reagent bottles from bulk containers via the retort and a hose plugged into the processor module while the user is fully protected against coming into contact with reagents Remote wax bath fill drain feature Optical fluid level sensor Active paraffin wax solvent removal feature that increases the life of the wax by extracting and condensing contaminating solvents Magnetic stirrer to gently circulate reagents ensuring uniform reagent temperature Programmable finish time for processing programs Three step retort drain feature adjustable minimizes reagent carryover Specimen processing under pressure vacuum alternating pressure vacuum cycles or under ambient pressure Four user programmable clean programs The clean programs automatically skip any steps not required to complete the clean Operating Manual V 1 2 01 2008 3 Instrument components and specifications 3 3 Standard delivery packing list The basic version of the Leica ASP300 S includes the following parts 1 Leica ASP300 Basic Instrument 1 Set of power cords 230 240 V version 1 Main
54. nly authorized and qualified service personnel may repair the instrument and access the instrument s internal components 2 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the institution which owns the Instrument and in addition the designated personnel who operates services or repairs the instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings Leica ASP300S 2 Safety Warnings Markings on the instrument itself operating instructions as defined in this manual must be followed when operating or replacing the item marked Failure to adhere to these instructions may result in an acci dent personal injury damage to the instrument or accessory equipment A Markings on the instrument showing the warning triangle indicate that the correct A Some instrument surfaces become hot during operation They are marked with this warning label Touching these surfaces may cause burns Transport and Installation Follow the unpacking instructions carefully to avoid damage to the instrument A cleaning must be performed prior to each transport during which the instrument may be shaken tilted or lifted Severe internal damage may otherwise occur A The instrument may only be transported in a
55. nnnnnnas 12 3 1 Overview instrument COMPONENTS cccescessesssecsceesesseseseeseesenseseesensesseseesensensessuseesansesensensatensensanes 12 3 2 Specific instrument options saoscesesssnescisnssancsanciaasiscirscesiestutarcactorvassuacse meee aeadansieteuaiaorstancstonedinsatenates 14 3 93 Standard delivery packing MISE s eessessseressrerererunururununununanunanavinnnnnnnnunununununununununananunununanannnnnnnnnrnrnnnns 15 e TEC PCa Da eE E E E E E N 16 35 Compatible reageNiS cor ceze ceeucerrs aca acusictersadacaineenmseantessatans ty stepataanasactani iiia caa oi ideii ronnan 19 4 Setting up the instrument ccccsesesssseeeeeeseseceeaeaeseseseeeeassesesenenaeaesesenaeaeesenenenassenesetanas 20 Ar SEES aa E E A AREE 20 42 EMEC WI GCal COMME CUON arrre tastserettadaroteatssnpoimasanenapatevmaaiuatsenasngcantassanashyteentsdbasstencttaaniaieesataaeneaciasnecarates 20 4 3 Installing the accessories ssisiisceccauscd juteasndniesnotunsiansaans oattdnwutosausduniinansancimensidiusieedsstvecnntyiadeanvigvanarvauneanine 22 44 Setting up the data connections e essssssresessrsrurenururururununanananuvunnnrunnrnrurnnunununanununananananunnnannnnnnnnnnnnnnns 23 AS ACOS eara AEA E AE 24 4 6 Switching the instrumenton tanisdonscscsptcbcnsnacnstsdapucdsdauansscisdediasteannonanatelaatndadiieiasiutabdidesantadtacadevudiagaeias 25 AT Touchscreen MUM LIONS ceesre panaia e a E E aR 27 48 TSE CKIISE TO TGS operato inaire r A D E 28 5 Ope
56. outlet socket ON e Switch the ON OFF switch item 42 Fig 4 located at the rear panel of the instrument ON e Switch the ON STOP switch at the right side of the instrument ON The ON OFF switch at the rear and the ON STOP switch at the side should be left ON at all times to maintain heating to the paraffin wax stations The ON STOP switch at the side of the instrument may be used in an emergency to stop the instrument during operation Favorites e The instrument will take a few minutes to ini tialize See opposite for corresponding touch Initialising the instrument screen display Fig 13 Please wait e After that the 14 Fig 13 Screen saver e Ascreen saver will operate turning the screen off when no key has been pressed for a user q Small Biopsies Finish ASAP programmable time Press any part of the touchscreen to restore the screen After having been restored the screen willbe inoperational for a few seconds to avoid acci dental activation of any keys Fig 14 Operating Manual V 1 2 01 2008 4 Setting up the instrument System Diagnostics Thursday 20 July 2006 11 35 42 AM The following functions can be selected 1 Access for Service Engineers only 2 Access INSTALLATION menu 3 Abort the current program 4 Abort the current program and delete the cur rent allocation of reagents to reagent bottles and retort 5 Delete all programs and reset instrum
57. ration roerne E E ae e pees O 29 5 1 Setting up the instrument parameters cccccecssescsseesssessesessessesescessesenseesesenseeceesesenseeessnseesenesenseneas 29 a RE EES 0 A EEEE E E EEE E E E E E E A E areata 29 o2 ACCESS IOV EIS mirourion e A NEA T Ona aeaiia 32 DISAIN STALA UCU TEN a A E A AER 34 5 1 4 Editing the reagent list seeseseserersrsrerununururununununanunanananannnunnnnnnnnurununununnnunununanunananananannnnnnnnnrnnnnnnnnnnnn enne 37 5 1 5 Viewing the program NS Et cctcae crease aneeleecntsectincuamvantesdaunati atnestigavatarantanaasetitedrenmtnanetehcinednaitbanarentaviedtecsteuts 40 5 1 6 Adding and or modifying Programs ccccccsccssssscsecsersssssessesesessesessessusescesersensessessnseesessnsensenseseesenees 41 Me FVE e A EA E A E 44 SAE E E EEE E A E A E E E E A E E E A E EE E 46 SEEE E E TN EC OUD r ENE cera A EE E E E EA E S P E 41 D2 Reagent Ma NING areren a a iE ondina anni 48 5 2 1 Fill drain reagents other than WAX ccccceccssessscsscssscsscessssesecsucevscsusessusesessucevecsusessusesessuensaseusesanenss 48 TAA PANU EVE VV AN serenas T E EA A O N 50 4 Operating Manual V 1 2 01 2008 Table of Contents So AMNI OCU A MINS een cue voa aeneastavergoces A E 53 53 1 Editing a selected DOU E dill rrii ana aaea agi 54 2 an aO e R E E E E E A made atueRaay 56 54 Reagent statis senoun onna E a ia a saree aani ES SN 57 VSCOM ONO a E E A 58 30 ORANO NOID oe e E E A TO OE E A EEEE 59 0 SOONE n E 60
58. s Press the button to activate the corresponding function on the Leica ASP300S touchscreen All buttons have a uniform design for easy Identification The buttons may contain text labels or graphical icons Smart Clean Buttons on the Leica ASP300S have different appearances depending on whether they are enabled or disabled A button is disabled if the function that it performs is not applicable at the moment Disabled icons have a thinner border than active ones as shown here lf a disabled button is pressed a help text will be displayed explaining why this particular button Is disabled Operating Manual V 1 2 01 2008 4 Setting up the instrument 4 9 Checklist for first operation i Once the instrument is ready to be switched on the menus below have to be accessed to set the required parameters For detailed instructions on each individual parameter refer to the On line help feature Screen Press button System Monitor Installation sim 7 Fig 18 Leica ASP300 S Parameter settings Enter carbon filter age warning and pump warning threshold Enter instrument name and select language Activate printer optional Set all parameters as required especially wax bath set temperature Check whether date and time are correct Add the desired reagents and edit the warning thresholds when REPLACE REAGENTS will be displayed Allocate a reagent to each station F
59. s Printer optional e Lift the flap of the instrument console 6 e Connectthe printer to the printer port 8 in the console via a standard Centronics cable Fig 8 e A suitable printer can be recommended by your Leica distributor e f conformance to electromagnetic interfer ence standards Is essential a specially shield ed printer cable will be required Other connections e For additional data transfer and storage the instrument console is equipped with a stan dard 1 44 MB floppy disc drive 11 and an RS 232 serial interface 12 Fig 8 Leica ASP300S 23 4 Setting up the instrument 4 5 Anti reflection clip Installation Instructions Anti reflection clip Function The anti reflection clip Order no 0476 44135 prevents any reflections that might otherwise be emitted from the specimen basket 3 order no 0476 34193 and that could affect the level sensor in the retort thus causing malfunctions Installing the clip The anti reflection clips are intended for installation on the specimen basket to prevent reflections of the level sensor e Insert the left lug 2a of the anti reflection clip 1 into the seventh hole from the left of the lower oval openings Fig 9 e Snap the right lug 2b Fig 10 into the seventh hole from the right with light pressure When inserting or removing the metal baskets into from the retort always do so carefully so that t
60. s cable D 1 Mains cable UK ST BU F 5A 100 120 V version 1 Mains cable USA C J Jumper cable mains Basket hook Specimen baskets assy including lid dividers springs and handle Remote fill drain hose Paraffin drain hose Funnel Lubricant Molykote 111 for valves and O rings Activated carbon filters assy Magnetic stirrer complete Paraffin wax scraper 4 Reagent bottles plastic 13 in instrument Condensate collector plastic in instrument Drip tray in instrument Splash protection Maintenance kit 2 replacement lids 9 o rings Maintenance kit for pump in instrument Sets of labels for reagent bottles set of 15 each Remote alarm plug Operating manual for Leica ASP300 S multi language 3 5 HD diskette blank DOS formatted on rear of console cover Demo Program CD ROM AQ m AQ Wa Part no 0411 13558 0411 27822 0411 13559 0411 34604 0476 34713 0476 34193 0476 34716 0476 34721 0476 43631 0336 35460 0476 34150 0476 34202 0476 35923 0476 34274 0476 34278 0476 34145 0476 34770 0476 35921 0476 35922 0476 35 92 6844 01005 0476 34712 Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete If there is any difference please contact your local Leica Sales Office immediately Leica ASP300S 15 3 Instrument components and specifications 3 4 Te
61. set to 0 Leica ASP300S 41 5 Operation 5 1 5 Viewing the program list This list Fig 43 displays all programs currently existing in the E ASP300S You can create A e Up to 15 processing programs ast retort cleaning programs lt 1 wax cleaning program EE e fraso N C C e E _ iG At supervisor access level op Senna an Routine Cvenuaght 1 a iah Prionty I e the processing program names can be edited Extended Cleaning program 02 02 Sandor rong Pan Wax Clean Program os O e new processing programs can be created and existing ones can be deleted Fig 43 Important New processing programs are created by copying existing ones Therefore the list must contain at least one program at all times Program duration cannot be specified It is determined by the total dura tion of all program steps plus the estimated fill and drain times To alter the duration of a program the duration of one or more individual program steps has to be modified i Retort and wax cleaning programs are predefined They cannot be renamed added or deleted 42 Operating Manual V 1 2 01 2008 5 Operation 5 1 6 Adding and or modifying programs Boo goo e e OO OS 0 OO 00 a0 SoCo oo a0 0 e a ppt chai te Fig 44 Leica ASP300 S Creating a new program Make sure you are logged on at supervisor level
62. ss OK to confirm i Programs can be started up to 6 days in advance For the program to finish at a specific date time the delay step will be prolonged accordingly Operating Manual V 1 2 01 2008 5 Operation Access the RUN OPTIONS function to display the options selected for the current program LEVEL CHECK enabled The lower level sensor in the retort Is activat ed The program will be stopped and an error message will be displayed when the level Is not reached RECIRCULATION enabled During each processing step the reagent Is pumped periodically from the bottle to the retort and back for continuous and efficient mixing of fluid The first pump step is carried out after 16 minutes all subsequent pump steps in 12 minute intervals PRE TEST enabled After starting the program the first reagent is pumped into the retort and back into the bottle to verify whether all tubes and valves are unobstructed STIRRER enabled Magnetic stirrer is ON PRINTER if enabled At the end of the program run a full run log will be printed out The options selected will be applied to ALL programs All run options can be enabled disabled during the program run if necessary The printer option will be displayed only if a printer has been configured for the instrument 57 5 Operation 5 3 2 Starting a program aa Retort OPa 20 C Wax GC Level sensor Mot fall Lid Clos
63. sts of 3 phases Phase 1 Smart Clean Prior to starting the smart clean make sure all reagent containers are correctly inserted and connected All internal tubing is cleaned with pressurized air to force any remaining reagents back into the reagent bottles Phase 2 Before phase 2 starts the operator is prompted to remove all reagent bottles from the instrument All internal tubing is again cleaned with pressurized air Phase 3 Before phase 3 starts the operator is prompted to insert an empty bottle into station 11 The tubing is cleaned with pressurized air for the third time remaining rests of reagents if any are forced back into station 11 Empty and clean all reagent bottles thoroughly before reinsert ing them into the instrument to make sure there will be no re agent spills from the bottles that could cause damage during transport 68 Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance 7 2 General cleaning steps Paraffin wax baths Warning Be careful as the walls of the wax baths are very hot and may cause burns e Wipe the wax baths and lids clean The lid can be removed for cleaning purposes e f they are dirty remove the wax strainers from the wax baths Clean dry and reinsert them e Ensure that the air vent hole at the top rear right hand corner ts unobstructed Instrument exterior Clean the instrument exterior as necessary Wipe with a damp
64. ting the instrument serialnumber is attached to the back of the Loko Wirio Miraka Carrs Daa ieee instrument 7 Fig Operating Manual V 1 2 01 2008 2 Safety A and use of other Leica products Make sure to comply with the safety instructions and warnings in this chapter Make sure to read these instructions even if you are already familiar with the operation 2 1 Safety instructions This instruction manual includes important infor mation related to the operating safety and main tenance of the instrument and It is an important part of the product If additional requirements on acci dent prevention and environmental protection exist in the country of op eration this instruction manual must be supplemented by appropri ate instructions to ensure compli i ance with such requirements This instrument has been built and tested in ac cordance with the safety regulations on electri cal measuring control regulating and laboratory devices In order to maintain this condition and to ensure Safe operation the operator must comply with the instructions and warning contained in this instruction manual i For current information on applicable standards please refer to the instru men s CE declaration and visit http Awww histo solutions com A The protective devices on both instrument and accessories may neither be removed nor modified O
65. to ensure that the effectiveness of the clean is not compromised 62 Operating Manual V 1 2 01 2008 6 Solutions 6 3 Troubleshooting gt emo Leica ASP300 S When a problem occurs in the Leica ASP300S take the following steps to diagnose the problem i Use the SMART SCREEN functions to individually test opera Check for error messages indicating the nature of the problem lf an error message is displayed press the Help button on the message for any information relating to the cause Check the Run Log for any messages that indicate the nature of the fail ure If the failure occurred during a program run check the Run Log for an indication of whether the failure occurred during the fill processing or drain cycles and the reagent station in use at the time tions such as fill drain pressurizing and evacuation opera tions Power failure lf there is evidence of power to the instrument i If you cannot identify the problem by carrying out the above Verify that the mains lead is plugged in to the wall socket and that the wall socket is switched ON Check whether the bridge cable is plugged in properly Verify that the power switches on the rear of the instrument adjacent to the power lead and on the side of the instrument are both switched ON Messages indicating that there has been a partial power failure indicate that power has been lost to the heaters but not to the main control elec
66. tronics Carry out the above steps to identify the nature of the problem listed steps call Leica Technical Service 63 6 Solutions 6 4 Typical fill or drain problems Fill and drain problems may be due to several causes 1 There is insufficient reagent 64 Verify that both the reagent bottles and the wax stations have suffi cient reagent 2 Insufficient pressure or vacuum The Leica ASP300S is unable to create sufficient vacuum for a fill or pressure for a drain in the retort Verify that the reagent bottle in question is correctly inserted in the reagent cabinet Verify that all reagent bottles are fully pushed home into their sockets Check whether there is some debris under the retort lid seal causing a leak 3 There is a blockage in the air or reagent lines Blockages in the reagent lines are typically caused by wax or tissue de bris If there is reagent in the retort that cannot be drained try to remove the blockage as follows i If you cannot eliminate the problem by carrying out the above Heat the retort to the maximum possible temperature consistent with the reagent currently in the retort if any Leave the retort at this temperature for atleast 15 minutes Whilst the retort is at maximum temperature attempt to fill or drain lf there is no reagent in the retort Run the extended retort clean program Use the maximum settings for number of clean cycles and temperature consistent
67. with the type of reagents solvents used in the clean program listed steps call Leica Technical Service before attempting to run any further programs Operating Manual V 1 2 01 2008 7 Cleaning and Maintenance 7 1 Clean programs You find the clean programs at the end of the programs list Fig 43 page 42 To display a clean program highlight the respective line and press EDIT The ASP300S offers three retort clean programs and one paraffin clean program Clean programs cannot be copied or deleted 7 1 1 Retort clean programs a fo 23 A clean program comprises a minimum of three and a maximum of four steps A om S o oe i a 1 Cleaning Solvent e E T 1 step from station 11 2 Cleaning Alcohol 1 step from station 12 3 Cleaning Water 1 step from station 13 Optional step between steps 2 and 3 Cleaning Water Detergent 1 step from station 13 ext Fig 73 e The steps of retort clean programs are listed in the order in which they are carried out fig 73 e The clean programs skip any steps not required to complete the clean If e g an infiltration program has been aborted during the alcohol step the subsequent clean program will skip the cleaning solvent step and will start with the cleaning alcohol step Leica ASP300S 65 7 Cleaning and Maintenance Editing steps 66 To edit a program step highlight the corresponding line and press the respect

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