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Service Manual Template - Frank`s Hospital Workshop

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3. Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 05 FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOL ONLY 4070513 MANIFOLD ASBY 12 WELL B 4 4070515 BOTTLE SUPPLY ASBY B 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOLONLY 4071001 OVRLY FRONT B 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOLONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 99125 LABEL 1 X1 2 CONTINOUS Go MAR
4. Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 0 TOOL ONLY 4070512 MANIFOLD ASBY 8 WELL B 4 4070515 BOTTLE SUPPLY ASBY B 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOLONLY 4071001 OVRLY FRONT B 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOLONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL 1 7771012 TAG S N WITH MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOLONLY 9001018 LABEL IN VITRO DIAG USE A 1 99125 LABEL 1 X1 2 CONTINOUS qr MAR
5. SLOTS NI SI 1SOd SIF WALI OS L39V88 Q TOJNIVI 83M01 001 d31S aayy 3HOQNVIS 40 NOLLV2O 1 6 d3 LS O1 43333 WOLLOS ONY dO JYYMOYYH JSOGNVLS L30V8 CIOSINVW N3 1H9LL SLOTS NI 518504 9I N3HM OS OQ OL L3s2VH8 CIOSINVW 31v1OH 19 1 335 Zl d3ls EVI SLOTS NI JYV SLSOd OF WILI OS 1 8 T IOJNIVI 001 4315 z 00 LON 1v 14 LIS LSNW 3H1 313 14WOO SI LN3ANSTTV N3HM 31ON QHONVHO LON INSWNSITV LVHL SUNS 38 O L 8 V 1Y SNI13A31 va day q 318vO UIMUVO 3H1 40 WOLLOS 3 LSNIVOV dN HOVE ONY 4 SLNN WYF NSLHOLL JA31 N3HM 8 MYV SLOdS 4 LSnrav NMOHS SV Ya Q3y VW SLOdS 1v 1SOd 4323HO ONY L30vH8 C IOJNIVIN 1411 IWYS LAN wyr XOUddV 39V ld JHL LV Z Wall HONOL S1SOd H108 OS MIYOS 9NIT3A3 10V 15 3AO8V 5001 07 NMOHS 8 SLOdS NO 51504 SIF OS NMOG 1332 Vii8 QTOJINVW H3MO1 CHYMYOd 3015 ANY 1 Q 104NIVIN 14 WIL 3VS 3HL NMOHS SV SNILLIJ H3AO Z WALI HONOL S1S0d OI HLO8 OS M3OS 31 LNOY4 15 NMOHS 3O Z WALD GAMYWW 51045 NO 150 91 Wall OS WYO ONISN LAXOVYS GIOJINVW H3MO1 80L d31S MOMsv WnnovA 13920v38 Q1O3I
6. AH311V8 JO 215 3ALIVO3N NO s GSIGSUS SSIMNSH LO MOVE dil 1134 V 3 AB IjO cuc JJ AS3 LIV8 NO HV3A ANY HLNOW 2 aes d3sn LON 9L SL SATOH 1 SATOH NI 31V907 Q4VO8 OL AV1dSIC SNILNNO V dIVL3a 33S 338 AV IdSIG OL 3Q TO 0 AV IdSIG aav SALON f N 989 E M D a NMOG O O tE _ 8 I 3418 3ALLISOd 6v02v INV HOIH olsa s 13 009 n m N ecd QNS ENJ O r ZINH L Sy io wog 33S 299 z SLuvd TWNOLLdO wae m J ZNY L o sii mimm minns 8 89 S z LS ozn 4 8 en dal mima 99 I gie erm HUHH a ia _ yh unm v 1 1 8 g en 4E zzo 8 U 9 A B Q B HHI ui Bes e 9 88 d Sn Tm lt R 3 La Z 3108 430 10 2 VINOT 515 unen i lal2 zH gs 13005 tc lal 20 a ml 17 JO lt seo B B Bon f 2 L 55 lal 3 t 7 Salal 6 amp I g Zl za 498885 er O aed I cmn rp gt S om S 131008 zn 8 8 er a ONE D SH E 3 IL 17 gt a B BE N 8 HHI a a sn ezn lid ill lt X M elsi a E gf omy 90 9 680 6 M d lo o5
7. d31S NI 35001 1391 SM 39S 9 NALHOLL 7902Z20v OL T3 TIVeVd S LI OS 920 07 1Snrav rZLzL SMAYNOS ONILNNOW 910 AHL LYASNI SNId H3TIVIS z AHL 2 0 200 dO WOLLOS 3 NI 3 OH LOTS JHL NONY SNIH3AAO 1 3 TIHM SNId 39HY1 AHL NO d31S 30V ld A8SV Q IO3INVIA HO 9L SIf vesc2o0v 20220 4315 33S JO LYVd SM3HSVM 041 3 MANOS 2161 9056 09 a 98767 AINO QNVH A8 NALHOLL 8066 ddlS L d3lS 33S d3lS 33S NALHOIL H3ONIJ LOLZL dals pre S207220v 890 20 3 8615 OL HSN14 SI LAVHS SHL LYASNI OL ATIVNS3IX3 ANY ATIVNHALNI HLOS 3384 LSNA 1902107 NALHOLL 38 LSNW A18W3SSV AT8W3SSV OL _ N 690Z 0P AYA 10HOO IV HLIM 51 HSVM 0202207 x 90669 H 9 3 q 9 v 4070516 AS Syringe Pump ASBY Page 1 of 1 5 23 Elx50 Strip Washer Service Manual 4070516 Syringe Pump Assembly rev B As of 11 20 00 Item Description Rev Qty Remarks 12062 SCR PAN 8 32X3 4 SS SLOT A 2 12105 SCR PAN 8 32X1 2 PHILLIPS SS A 4 14041 NUT SQUARE 8 32 SS A 4 16002 LKWSHR 4 HELICAL SPR SS A 2 16016 LKWSHR 8 HELICAL SPR SS A 10 16067 WSHR 177IS X 50 OD X 062 SS A 4 17059 WSHR FL 188IDX 3750DX 049 SS B 6 18034 WSHR SPRING WAVE 4 SS B 2 19112 SCR
8. TIYIS eese A8SV EVN 14315 335 A8SV TISA 91 Q IO3INVIA XO8 NO LINN 3O 8 TvIH3S 38 AS8SV ONiddIHS XO8 40 401 3dvL 91016 OL d31S ONbIOvd WNOLLIGGY SV z9019 H1V3N38 P8016 A1ddV oLsozor Ova 3 8016 NI 39V1d 13S A1ddnS dVYM 318808 NI LON SI AlddN S H3MOd 31 4 XO8 WNISIHO 1 QM vOSIG 29019 ova 8016 39V1d 48 9 4315 33S 335 OVE 8086 NI 30V ld 9 9315 335 A8SV Q 1O3INVI OVE SIHL OLNI 9 6 HALTS Lnd 1190209 13S 9 31SVM 199 90 dYYHS LON OG T38v166cvv SNIddIHS 6 086 DILSV ld V OLNI WAHL ONY 5311108 5150 0 WO 4 5409 3AOW3H YAO LSIW S311108 SN5ld LH3SNI ALINHOJNOO JO NOILV3V 1930 9 WWANVW 335 311108 PAE TWNOILdO 5 150 0 YANNI L L83SNI MOVE ANM 3H1 TIV LINN AHL lt NMOHS SV OL NI YANNI Li LH3SNI 39V 1d NMOHS SV QHVAWHOJ AVM 3H L TIY OLNI LINN 39V 1d S 4315 33S SNIddlHS LYOddNS H3l4l1 32018 ONITIVLSNI M3ldv 9V8 NI LINN 38 98086 YANNI Hit LH3SNI XO YANNI LUASNI NMOHS NOILISOd OL WYO NYNL ABSV YINIYLNOO NIddIHS 100605 JO pl LL d3
9. 3 3 NOTIV 3ALL931O08d AV1dSIG SNIOQTONI AINO AV TH3AO dO HOWE Y3dYd 3SV3 38 3AOW3H 3SVO NI LO TS RSA OL S318VO AV TH3AO LH3SNI y 33S H3HSVM 1 QHVOBA3M ATHAO INIWNONY L IIVL3ad A18W3SSV Ol LoszZor I ABSV TVOINVHO3W 31 31vonign 1090 0 OL asn NMOHS SV 6867 OLNI HSNd 9 OLNI L3SNI 8 066 686 6 NMOHS SV 9If NO AT8W3SSV 1N3IHO 9r 0 0 GHVOBA3M XTHAO OL 30930 0830 HO NNOO A8SV OL SO NNOD A8SV 1511 ASSV r I H 9 3 q 9 8 7 Wshr 8 Well Page 1 of 4 4070005 AS Final ASBY Stri Assembly Drawings 5 4 8 5 NW SV S00020P TI3M 8 H3HSVM dl31S A8SV 1 0 BATWA IAA V vlad 335 180 20 Loszzov GNVLS AT8W3SSV IHL NI SI N3HM Q3N31H91L 38 LSnIN SMAYOS ONILNNOW LINN OISV8 OL WOLLOS 3 83AOO dO ONILNNOW N3HM x 313 1dWOO SI 9NILS31 N3HM 138V1 A1ddV NZ S31ON ozr 000207 5 5 b 00 0 0 er 0 02 HO NNOO X98340 SHIM HO NNOO 85 OL 1511 ASSV q vlad 1802 0 1 152 0 snidva ASNIVoV NOV Sr86r NMOHS ATNO
10. 89d NIVIN auva S VAOBddV auu 8660 0 60 MSOONIM ans 866 m n an ONI SLNSWNYLSNI 69 162 689 489 ve E NS 30 80 59 o ce a on av Lx wo 3 av L5 uy x 9 E 9 BVI 30 am ow a ow ow z s o aw so sw sw 1 owt ov ny nv ow uo av S svi S swt Ww ew ama i yw r m aw uw OiQ46ZWv ziy za ev a evi io zo ev uv uv uv Tow ovosszny oa w ow ow ow ND 3 s w m bioa w m d oui 5 vw oszi ev 5 AMOW3N z AMONGN 7 vi z ivi z ivi z ivi v 8 wr nvas v E Hsvia w LL svi M vi i dv E ia MOT 5 ww 20 62 6 av Le sa ov ov 91 SZ Suv 82 82 n m 90 82 sa ev sa sv sa sv sa sv sav ZL Z oowi 50 22 S vI sav ZZ S 50 Z SL en ow w w w 90 81 vav 92 a a evi E uv een 209 6 52 A ww 92 A Fn sz uw av za svn zv za m Z ev a ez ew ia 1 ia 92 10 22 6 iv 22 e zz e a a o
11. OGY V Vlad NOISQHIX3 CIANO LSIW 3 30 3963 3GISNI 099 ONISN 35SV1d NI QNO8 197 ANY TOHOO IV HLIM NY319 93 Q3HSINIA d AWOS SNIWIAIH L A8 S3O3ld LNO JHL ASIGOW 3QV 18 AdVHS V ASN c WE S3Oald OML LND L 2 3di INO 100 7 3 388f1H THNNVHO 9 829 8 1 19 LSIN Y34009 W W FF LSIN Y34009 JO FOVAYNS 3GISNI OL 04186 z Sv8 OLNI GLE X ZE OL HOS 9686 JOVId 0 g 1VLad 33S 6902207 ISIN H3AOO 6S0240v TSIN HV3H H3AOD AHL dO 39Q3 ACISNI JHL 393 SIH L ONITHW3SSV N3HM I g AL 62086 1 OVE NI ATHWaSSV 4 3 3OVTd t 3AIS3HQV 31112000 66099 40 INnOWV TIVWS v ONISN QNOS ONIGNOS 30338 ANG 131 JOHOOTW 1AdOHdOSI HLIM SSOVAYNS NV319 JSV3 133 GION OL AHVSS3O3N dl HLO 19 NOLLOO 3 HO 3AO19 NOLLOO V 110 22099 HLIM NV319 NZ SALON TIVLAG NI NNOHS SV 3904 8 dOL NONY V ISIN H3AOD AONIH dO dO HLIM LSIN 3 dOL NOV LSIW VA XZ 33S 0 q 9 8 V 4070517 AS Aerosol Cover ASBY Page 1 of 1 5 25 Elx50 Strip Washer Service Manual 407051
12. an esee aee eidem ate ate ideis 3 1 Carrier Axis Home tii re b as eo pa a iti ees 3 1 Manifold Axis Home tee UTE IRE TER NER EE 3 1 Syringe Axis Home e e E e TESTI ARE CENE cus ERE 3 1 Verifying an AXIS Re e e ERE e eee re ee rte dee 3 1 Manitold 3 5 sam Ibn eli pieta 3 1 nai ante or ee E a eats 3 1 Cartier EE 3 2 Ettot Codes EE 3 2 Noncfatal ettors ien ete e eie 3 2 bat l ertors eere C Uit a iru tuba n 3 3 System Contigurati n s 5t Neo ton tette b RN eh e 3 3 SECTION 4 4 1 eR 4 1 BON LOO US Zo oq Ro rr e E 4 1 Non Mandatory Field Changes eee ie eene 4 1 OU ee E e per OA 4 1 4 2 SECTION EE 5 1 Assembly Dr aWwInl9 oerte Q base iue 5 1 4070004 AS Kit Slider Upgrade Page 1 of 1 sss enne enne nennt nennen nnne 5 3 4070005 AS Final ASBY Strip Wshr 8 Well Page 1 of 4 sse enne nnne 5 4 4070005 AS Final ASBY Strip Wshr 8 Well Page 2 4 5 5 4070005 AS
13. csccssccsseesecsceeseeeseeeeeeeeeesecesecesecseesaecnaecaaecseeeaeeeneeneesereeerenrens 7 7 ELX50 8GS Strip Washer 8 Well Generic Short 7 8 ELX50 8VS Strip Washer 8 Well w Valves Short Dispense ns 7 9 ELX50 8GVS Strip Washer 8 Well Generic w Valves Short Dispense 7 10 ELEX50 12 Strip Washer 12 Well 55 tut e RU o bte tite io mt 7 11 ELX50 12G Strip Washer 12 Well Generic esses eene nennen nennen nennen nennen rennen tenens 7 12 ELX50 12V Strip Washer 12 Well w Valves esee nennen nenne innen teen enne 7 13 ELX50 12GV Strip Washer 12 Well Generic 1 7 14 EL X50 16 Strip Washer 16 Well te ere Re 7 15 ELX50 16G Strip Washer 16 Well 1 7 16 ELX50 16V Strip Washer 16 Well w Valves enne nnne enne eren enne nnne nnns 7 17 ELX50 16GV Strip Washer 16 Well Generic w Valves nn ener 7 18 4070001 Accessories Set Bottles and Tubes sss enne nennen 7 19 4070002 Accessories Set Generic Unit nece cette tete 7 20 4070018 Accessories Set 384 Unit eene eene emen en en
14. HON NOLLONGONd 866 XOR ONY HOH ise AS NYG S3HONI NI JYY SNOISNINIA CC 5 G3I3IO3dS 36 953 a Q aj Q Oo GALWAS ATINA SI WAS IHL 1VH L ONINVAW WYOSINN 38 LSNW dY9 SOVAYNS ONILNNOW IHL 6066 1V3S IHL N33M138 dV9 JHL 3A 33S8O Olt WO 9702 0 SAOWSY NMOHS SV 3 19 NS S svozzor mc c N N Ng 6 he sv0zZ0v a 5 nS NILNNOW 6066 30vdsns ONILNNOW 6066 9 o Ly 3111901 85 39 LLSZZOY Sv0clov daovauns 38 ISNW 9 ONINdS IHL NO ANY 139 LON OG NMOHS SV S3OV1d 8 31LLOO 1A 1ddV 39V ld 3SN3dSIG 2 667 HLIM 099 JO 311108 WYY9 0c 35 V L dals 33S 3ovdsns 9NILNnOW 1v3s 60669 Sv0cZ0v H3Q Il OHS NO Q31V3S ATINA SI WAS 3H1 NMOG SS3Hd dO 85 1V3S Olf LLSZ OF L d3lS 30 4 A8SV Iwas Ir 1192209 SS dOL HON38 5 NO Q31V3S SI WAS ILLND 55384 ACIS SNIHdS 6066 dO A8SV Tv3aS 9Ir LLSZLOv 3SNV I4 JHL NO S IVIH31VIA SNINV3TO 553815 ON 3AV31 135 ONITIVLSNI 330438 ANC 131 1IVdOHdOSI HLIM 39NV 14 NV3T1O N SHLON r H 9
15. 3sN3dsia SSFINIVIS 538 1 3SN3dSId NO 1231 IANS L B SNId LNO 8V319 OL 2702 0 SNTALS ASN ore 6 S193430 SOVINNS ANY SHYNE SHOIN AAISSHAY JO A T d 33834 38 LSNW 538 1 40 3QISNI S39vdsns TIY B 3 ATIVNYSLNI S T 2 3313 LSNA 38 LSNW A18W3SSV AT8W3SSV ow OL HOlHd AHO TOHOOTY HLIM SLuvd HSVM NP B 60121 0L SALON r H 3 3 8 BY 12 Well Page 1 of 1 4070513 AS Manifold Assembly Drawings 5 20 4070512 Manifold Assembly 8 Well rev B As of 11 20 00 Item Description Rev Qty Remarks 12105 SCR PAN 8 32X1 2 PHILLIPS SS A 8 2872220 MP TEFLON DISPENSE TUBE ASBY MP D 0 DOCUMENT ONLY 2872538 JIG 0595 DIA TEFLON TUBE DRAW A 0 TOOL ONLY 4070513 AS MANIFOLD ASBY 12 WELL B 0 DOCUMENT ONLY 4072010 MANIFOLD WASHER 8 WELL G 1 4072011 TOP WASHER MANIFOLD E 1 4072012 GASKET WASHER MANIFOLD A 1 4072041 TUBE DISPENSE TEFLON B 8 4072041 DG TUBE DISPENSE TEFLON A 0 DOCUMENT ONLY 4072042 STYLUS TUBE ASPIRATE 0 TOOL ONLY 4072043 STYLUS TUBE DISPENSE C 0 TOOL ONLY 4072057 PACKING MANIFOLD A 1 4072507 JIG TEFLON TUBE CUTTING A 0 TOOL ONLY 4072508 JIG TEFLON SLEEVE INSTALL A 0 TOOL ONLY 407251
16. 4a Rete ee UR aee 1 4 Equipment Overview xut cR acon Ree AG AG itu aed e Ae tiet tete RU QN Ee Nd 1 5 SECTION T E A T 2 1 ridge 2 1 Principles f Operation scene caine ated RIP ROI e ERE De CURRERE deg 2 1 OVENI EW es ace Ie ea ee tu e ae 2 1 Hardware 5 nete aee REGE e 2 1 iouis M 2 1 Mannitol discrete CLTC 2 1 Carriers nt sushi lace han iamen 2 3 Carrier to Manifold P sItI nIne a nnne trennen aasawa enne nnne 2 3 Manifold Vertical Positioning 5 e ete eiecti eee RIO Teo eU eee 2 3 Positive Displacement Pump m n eet een re 2 3 Macuuin PUMP oae i Ee AU Te PEU SER u AN OUR EE B QUERER RM E 2 4 Vessels and Connections eng ive pa ie e i s 2 4 Display and Keypad siete e RN DRE DES QE UU DRE UD n E e rU ta t det te eed 2 5 Power Supply x diae e tiii s eee ta eee EET EE 2 5 Decontamination s see Ie e tte d ER ate 2 5 Aerosol eei e eie Pete eee eee 2 5 COD ADIE NI Goa ie eate hs 2 6 Configuration Software z s unu D S e e EN mat t eia eie t ett ead 2 7 SECTION 3 1 Error M 3 1
17. 82d NIVIN aia S WAOHddv auu 8660 POPSO DISOONIM 866 Vd ONI SLNSIACIHLSNI M3l Ol8 or Eri sao z ano FJ aneo wya D lt com s E E 1 5658 p l 13654 L zao E 1 MM DT ma Y 1 1dNS SONO 90N MEE M ONTGAS 4 tao E RA ou e ia uk zo sz 0 za 3 L 3 z3svud D zan n sa 1 mm BA 02 en 109 E iNOS shee zi IWW LWA 102 OLOdN 3SN3dsia D 8 u RUA oa 5 FE Ex nk 519 A 99 z negon 619 LL ovon e 13834 M sa 214 SONO 80 1 Ww Fx 4 en i 4 3 5 oveewed i H an ses zo EZ ta zao i za z L d sa D zan m sa mm HA zn oz r
18. ONI SLN3ADHISNI L M3l Ol8 or Ho aNd 1 4 e I 5 5 e I e 9 oy ME A ay a r oF e 35 5 MD s zn e fA s d Y w za 2 D 3 e Ww n g z z M H 5 o i 1 9 s z 9 H ant uus dois w lt 015 or ES T CQ D ot az vi lacs D m H 39 39 30 soni mm ven I 20 xo J ox MLNS bs uva 6 He 5 5 4 2a 3 sa so sa 5 v v z z v i L Mw om T H L r a zo za XIX LWW QVdA3M q Noawnd 6 s oa 29 wg ant 86 8 viva nh Age ino L so 880 z 0 99 ano aot anode lew S31V9 VH1X3 8010 j A LI zu wez E E s HOLINOW GOODS H3MOd an9 AS 4 Lo moot 99 EE e v 3100 No 90 Huozz H3MOd 21901 ino NIA z d Suo AS2 S Ad 9001 so vecsbi Lo zooxna ur MEE x z SYOLOW 34 318 H3MOd ner ae m za n 400 SC Main PCB Page 6 of 6 6 11 Elx50 Strip Washer Servi
19. Se 205 d s 9 ano GND SIN N30 4 s 104 4 rJ SON DE M on ENS si 17001 go vvv 90 son 4 son s MEE S914 501 hid 224 rel aHa pe Mm e n 4 ou m m a P 1500 zz zs ge 0500 E oso B 44 0510 os 02 398L008NL g es gt y 79 4 s erwies z mn T T arves m s stav yor I M V nay 9 S S s sao z k E tao ziav e z n 2 ede n orav ide z sav 58 LE 9 suedunr lii voneunbyuog 10v Ix sav kd sina tav SE z 99 sov vE Y zov D T t TINH 00 9 n 4 aw E 44 asns 10013939 FT 1noy15 90 kd SINS Gi tezisa 19 apt ES qni an 29 s89 L 160 a 90 i n TL ino Le ano 2 i Schematics 4070400 SC Main PCB Page 1 of 6 6 6 28 00007 9 30 133HS ONIMVEG aos w m
20. ente nna 7 21 4070019 Accessories Set 384 Generic Unit esses eene E enne tren 7 22 4070520 Aerosol Cover Assembly Generic esssssesseseeeee ener nennen nnne ener nennen 7 23 SECTION P 8 1 Recommended Service Replacement 8 1 SEG TION ECL 9 1 Proc dures t 9 1 ix Section 1 General Information Purpose and Scope This document is a service manual It provides technical information for qualified service personnel to assemble and test the functionality of the ELx50 line of instrumentation and assumes a general understanding of the Bio Tek Instruments Inc ELx50 Automated Strip Washer product line This manual should be used in conjunction with the Operator s Guide for ELx50 Automated Strip Washer Bio Tek part number 4071000 Certain sections of this manual contain copies of documentation that is currently in use at Bio Tek All documentation is subject to revision over time Schematics are included in this manual if component level repair is necessary Assembly drawings are included to aid in the assembly and disassembly of the ELx50 If you need further information please contact the Technical Assistance Center Mail Bio Tek Instruments Inc Technical Assistance Center Highland Park Box 998 Winooski Vermont USA
21. xz 00566 xz 4 01227 G3r1ddnS LAN g 1 1 33s v 1IVL3 19383 STSAIMS 15813 GNA OZOZE Z vaavo SIHL 1H3SNI 99061 86867 xv AT8W3SSV OIf 1052 0 S302 15814 9 SIHL 13SNI LAN HLIM HSNI Xv xz SI diL M3Y S 0091 66867 xc Xv 68161 saui 300801 1SNIVOY NOV Serey 0 20 90 F 00 21 ONILNNOW 310338 1OHOOTV 39v4nS NOM dIdLS 06299 6 Wvodrsa3ans eir 3uvMaavH 209220 GASNNN GHVOSIC 01227 3 1d3N a3riddns SYVMGYVH SNILNnOW AYYOSIA HO1VIW N w mi 0070207 8 3 O1 66099 3111001 ATddY N 17 00020 ZO0ECtZ er 000207 V 318vo NMOHS SV Sif No SQV3HH 90099 3111901 A1ddV oL MOS3Q JAIM 1N3IHO 00 20 HO NNOO A8SV OL HO NNOO A8SV WOU S31ON 1511 ASY I H 9 3 3 9 echanical ASBY Paoe 1 of 3 4070507 5 5 9 Elx50 Strip Washer Service Manual sw s03 suenan 33voS LON OG 3 l via3a 8 Sv 205040 q SUVMOYVH LSU 5005 08 LNNOW 310N ASV TVOINVHO3W vauva 94315 L T Na a0vAuns es 1 Q XEXIOLYYINONY eio Sores ENT INE S245 NT ey SNOIRONIG TY ME A8SV WYO SE SENSI AL
22. SILON oX ZFX HVT0SNV SLOFX 8690 0290 LA ISOONIM ONE S3ONVH3101 NOILONGOYd 866 66 9z z HLINS S A8 S3HONI NI SNOISN3MWIG SLNIWNYLSNI SYNLWNOIS 31519385 ASIMYSHLO 5597131 yB O BO 94315 94915 21016 PERS PR 1 35019 XO8 LINN Q399V8 30V1d NOLSId OLNO 307S ATINSSYVO AMSA 8180 07 P di Q OVE OILSW1d Y OLNI LINN Q3HSINIJ SHVMQMSVH ONILNNOW 330338 LHVHS YOLOW NYNL A8 LHVHS OLNO d31S M3MOS C0ZZ0v NI 31OH NYHL YOLOW 10 LAVHS GS0VSYHL LH3SNI 3318vO z d31S L d31S Og 2 us 3ov4dHns SauvMoL 3119 1v14 4915 2061 Xo d Lvov xz 09182 2 veosl al 2 SZOZ Ov x Ivi 9 91091 LOv6L xv xz 2202205 FA ATS 69024 gt Via Z0091 WY 2 xv 0 29091 7 azon oe 1VOO NIHL V 909 Z B q ATIQJ3HVO ASH3A ANY 3A331S SALLO31OSd SAYNLOVANNVN WO 4 0906205 SAOWSY dq3aN31X3 ATINA SI NOLSId OS HOLOW SSAO 1o SV3M SNIIGNVH N3HM 0906205 7 1661 Gi WD R q 9 das 3as xr YG 90121 j H 4 3 q X g v Assembly Drawings 4070524 AS Syringe Pump ASBY Medtec Page 1 of 1
23. SV YANNI 1 LH3SNI 30V d 29019 H1V3N38 8016 Alddv SV AYM SHL 0150 0 3 88 8 8016 NI 39V1d OLNI LINN 39V1d 13s AVYM 318808 NI S 9315 das LON SI Aldd 93MOd dl 4 XO8 JHL OYYOSIQ 29019 ONIdd IHS LYOddNS YILI 40078 ONITIVISNI 8 3313V OVE NI LINN ova 3188ng 1340vu8 8016 NI 32V 1d G 103INV OLNO HSNd 9 280 9 4315338 1 CTOSINV OL 3 0018 NI 3A0089 ATLA 33s NOLLISOd NMOG 3H1 NI SI 3 IHL OS WYO NYNL 6 33s op SL Ove 8096 NI ovd W V 916 YILA 1nd Li LUASNI nsoZop Agsv gToJINvW YANNI NMOHS 195 ONIGNL 31SVM Ci 13SNI NOILISOd 1 T103INVIW OL WYO A8SV ONIddIHS L00 20F JO ama a7 OL SALON H 9 3 3 q 8 V 2 2 4070005 AS Final ASBY Stri Assembly Drawings 5 6 o grass 13 p pur avos LON oa N SV S00020r TIIM 8 H3HSVM in mam 1 A8SV micis NOILONGOYd 091915395 3SIMHSHIO SS INIT YELFOK NMOHS SV XO8 NI 39Y1d OLLSW1d A8SV H3AO9 TOSONSY SaOv1d Z WNWININ WOLLOg L dals WO XOUddV LINN 40
24. Software The ELx50 washer is operated by on board software controllable through the washer s keypad This on board software referred to as the basecode has an identifying part number Your washer is installed with one of two possible ELx50 basecode part numbers 4070201 or 4070230 Each part number in turn has an identifying version number If you need to contact Bio Tek Instruments for technical assistance you will be asked for the part number and version of the basecode currently residing on your washer e locate this information press these keys from the washer s main menu UTIL gt TESTS CHKSUM The basecode part number and accompanying version number will appear for a few seconds followed by additional configuration information The display will automatically return to the main menu The 4070201 basecode supports the following features e Compatibility with the 8 8s and 12 channel manifolds e An easy to use menu driven interface Ability to create Wash Prime Dispense and Aspirate programs for automated microplate washing e Storage of up to 75 washer programs e User definable washer program names for rapid recall at program run time e Configurable program parameters such as variable soaking multispeed shaking bottom washing and crosswise aspiration Pre defined washer programs that can be copied and used as the basis for new programs e Pre defined maintenance programs includ
25. 5 30 4070524 Syringe Pump Assembly Medtec rev A As of 11 20 00 Item Description Rev Qty Remarks 12062 SCR PAN 8 32X3 4 SS SLOT A 2 12105 SCR PAN 8 32X1 2 PHILLIPS SS A 4 14041 NUT SQUARE 8 32 SS A 4 16002 LKWSHR 4 HELICAL SPR SS A 2 16016 LKWSHR 8 HELICAL SPR SS A 10 16067 WSHR 177IS X 50 OD X 062 SS A 4 17059 WSHR FL 188IDX 3750DX 049 SS B 6 18034 WSHR SPRING WAVE 4 SS B 2 19112 SCR SOCK CAP 4 40X3 8 SS B 2 19143 SCR SOCK CAP 8 32X1 2 SS A 4 19397 SCR SELF SEAL 10 32X 75 A 1 19401 STANDOFF M F HEX 4 40X9 16 SS A 2 28160 OPTO SENSOR MEDTEC A 1 4070518 SEAL AND CYLINDER ASBY A 1 4070524 AS SYRINGE PUMP ASBY MEDTEC A 0 DOCUMENT ONLY 4070525 CABLE SYRINGE PUMP ASBY MEDTEC A 1 CABLE F 4072023 MOUNTING BRACKET SYRINGE PUMP 1 4072025 NUT LEAD SCREW B 1 4072027 MOUNT SYRINGE PUMP PISTON D 1 4072060 PISTON SYRINGE PUMP POLISHED A 1 4072506 JIG GLUE SYRINGE PUMP B 0 66006 LOCTITE 242 BLUE A 0 AIR 66039 GREASE LINEAR WAY A 0 A R 91012 BOX 5X5X9 200 TEST WHITE A 0 98083 BAG 9X12 PLASTIC 2MIL A 0 Elx50 Strip Washer Service Manual 5 31 Ag awa 003 Jen 3 4D sme sma 31508 LON oq s g SV 4c8040Tvr 5 STOTT ON OR
26. CAP MOD 2 HOLE 89MM PP LINED BTL 2QT LG MOUTH PE FTG M LUER X 5 32 BARB PP TUBING SILICON 1 8 ID X 1 4 OD BAG POLY 12 X 18 Item 4070515 AS 4072038 48370 49904 67262 98079 Assembly Drawings 5 22 L k 133Hs vIe anvos m 53943 Sung A8 003 1 0 08181 37005 LON OQ se sess v g SV 4c8040r 9 ETET 548 66 2119 sozze 349 8 SWISINSWDWISNIYSEOIS 40 9NIMVHG azis 1N3SNOO NALLIMM SS38dX3 LNOHLIM SU3HLO 1 A8SV TI3M 9L SUSHIO G3SOTOSIO 39 THS O3NIVINOO JHL NON 11 ON SLNIWNYLSNI 931 018 HSINIA TIVI LV dO ASLO td ONY sun INOS amna te Hsmia3ovauns I XTAXIOLUVINONY 9109 60 0P50 A BEL L JNOSNHOII S3ONVH3T101 QNV MOM 6666 6 HINSE ANYO S3HONI NI SNOISN3WIG 77 Y 4315 IN NNT GN 94 3uniwols 021310395 SSIMYSHLO SS31Nn 9 5 LNO 3SV1d 3 9 OVE 21086 NI 83 Y ddlS 33S SQVAYHL AHL OL 76299 3111001 HalSVv3 ATEWAS OL 1OHOO IV HLIM
27. 1311 XZ SM3HOS LN3WN ITV xe 0 AWLNOZIYOH 1 3903 LN3WN I TV Sif LSNIVOV s zee6 1v 14 SI WYO 3H1 OS 1431A8SV WvO HOLON 1411 HSNd 0 d31S 3 94 1avHs OS OG OL lr 3ans 1504 35 8 aNnosiv SI WEY 9 30 QN3 JHL NI 1015 3H1 OS WHY 1N3WN9 ITv 30115 1509 NMOHS SV LNOW 1 LJVHS OLNO Ir 0 4315 NMOHS SV NHn L NMOHS 3H3HM XOYddY 810209 HSNd 9 9315 NMOHS SV 1N3IMO 85091 SH3HLO TWV ZL 8 0973 S 91 09X13 9L 09X13 SNI38 LINN IHL v 9315 das HO4 SI IHL OLNO 329018 19JYY09 JHL TIVLSNI V4 d31S IN3WNOUIV ABS V WVORIOLON 1317 4318 L 2 d3lS A8SV WvO HOLON 1417 d3lS 99315 2 NI 8102 0 902 0 NO NMOG ONIHSNd 6002 0 3310 OLNI dVNS IMAM S00Z20r 34 19 xz 3 902 07 LSNIVOV 7686 NMOHS SV VERB AN3W3NOIv WYO 3AISSHQV r90Z 0r LH3SNI 30S 8 SNILJITOIP ONY 9NDIOV8 JAOW3H E 33H 59091 8 xe 0SZ 0v OLNI 666 TIVLSNI 10220 440 3dIA LAVHS 3HL 66099 JO INNOWY TIVAS V 3SN S002 07 NO AT3383 STIS 8102 0 959091 8 JYNSN ANY ATIVOLLH3A L3VHS TIVLSNI ONIdS JHL TIVISNI NOA 30438 NMOHS
28. 8 LON OG ITE TTE N SV S00020v q 181 43S somma xe TIAM 8 H3HSVM NM d31S nos 339 ASV TVNId 7 AINO SLINN SA Ve Zovauns ld SNId OLNO id P c HVv3ddvsla Suva QS VOSA3M ATHAO ce zr su3avaH 9438 S3HONI NI SNOISNANI JHL TILNN 340 MOVE Hadr TIVLSNI EMUI 79079345 SSIWISHIO SSSTNIT 3 TINS OL AV1dSIq NMOG 40 993 8 19 OL 9 MaI3WOLLN310d LLY ISN H3HSVM 9 3131 SAOWSY 0000p IHL YIMOd ANY 29019 AlddNS M3MOd V H3HSVM 3HL LO3NNOO d31S SLETdNOO SUV d31S z 33S 00667 ONISN 9310 4 S3ATVA Silva HIS 1 SI LINN 41 N Sz z z z ONITIVLSNI 310338 1OHOOTV BOVIYNS ONILNNOW 40 NV310 SALON nos 33S Xb NAHL 340438 3sva OLNI TIVLSNI 6867 NOE das nog 336 N we JAVA OL 1 13 zL En Sc db 8 amwa L ZV3001L 29219 33S 29219 xz wos 335 G uiv 10 v 21091 NOLLVLNAINO 31 nog 335 a18vo 79229 ZV300 211529 335 29610 292 9 woe 555 E SATWA OL 7066 21900 29219 NMOG MOLLS 40 5 1331 9 ASNIVOV AV TH3AO HSNd 9If dO 3903 HLIM AV TH3AO JHL 40 3903
29. TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 99125 LABEL 1 X1 2 CONTINOUS MARK WITH MODEL NUMBER Elx50 Strip Washer Service Manual ELX50 8GS Strip Washer 8 Well Generic Short Dispense ELX50 8GS Strip Washer 8 Well Generic Short Dispense rev P As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070519 MANF 8 WELL SHORT DISP ASBY A 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 H
30. 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 98011 BAG ZIP LOCK SMALL 0 98083 BAG 9X12 PLASTIC 2MIL 0 99125 LABEL 1 X1 2 CONTINOUS 1 MARK WITH MODEL NUMBER Elx50 Strip Washer Service Manual 7 17 ELX50 16GV Strip Washer 16 Well Generic w Valves ELX50 16GV Strip Washer 16 Well Generic w Valves rev G As of 11 20 00 Item Description Rev Qty Remarks 16077 WSHR ELT 174X 375X 030 003 A 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070019 ACCESSORIES GENERIC 384 UNIT B 1 4070020 DS FINAL TEST PROCEDURE ELX50 16 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070515 BOTTLE SUPPLY ASBY B 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070527 MANIFOLD 16 WELL ASBY B 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072073 PLATE MANIFOLD GLIDE C 1 4072077 STRIP GLIDE A 1 4072079 SPACER GLIDE STRIP B 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WA
31. 12 channel 12 well strips 96 well plates 16 channel 8 well strips 96 well plates 384 well plates The 8s channel manifold has shorter dispense tubes for special applications The ELx50 washer supports the washing of standard flat round and V bottom microwells Note The 16 channel manifold uses two aspirating tubes for each well when used with 8 well strips and 96 well plates For these formats it may be difficult for the tubes to reach the very bottom of round or V bottom wells possibly leading to higher residuals An ELx50 washer can be reconfigured to support a different manifold The level of difficulty for reconfiguration depends on the current and desired manifolds Appendix C in the ELx50 Automated Strip Washer Operator s Manual discusses manifold reconfiguration Carrier There are two possible plate carrier assemblies p n 4070526 for the 16 channel washer models and p n 4070508 for all of the other models e 4070508 holds 96 well plates and 1x8 or 1x12 strips and may be used with either the 1x8 or 1x12 manifold e 4070526 holds 384 well plates 96 well plates and 1x8 strips and may be used with either a 1x16 or 1x8 manifold Strips must be placed in their frames to be held by the carrier The carrier positions the strips beneath the manifold where wash solution is aspirated from and dispensed to the test wells The carrier 1s removable by the user and is constructed in a manner which allows for and with m
32. 8 X 8 1818181 m 7 55 K sey B8B BB 6 r E 6 4 o 3 X Ble gt S B i n E ozr r er vr j N uu di 89d NIV WH OL T Q 4070400 Main PCB rev E As of 11 20 00 Item Description Rev Qty Remarks 04018 LCD 2X24 SUPERTWIST DISPLAY E 1 DIPLAY 12089 SCR PAN 2 56X1 4 SS PHIL A 4 TO MOUNT DISPLAY 16001 LKWSHR 72 HELICAL SPR A 4 TO MOUNT DISPLAY 21010 DIODE IN5821 SCHOTTKY 30V 1 4W B 2 CR4 5 21013 DIODE SF21 50V 2AMP A 1 CR10 23099 VOLT REG LM2575S ADJ A 1 Q1 25108 IC LT1017CN8 COMPARATOR A 1 U31 INSTALLED IN 25135 IC 80C186 20 UP PLCC B 1 SOCKET U48 25136 IC DS1233 10 ECONO RESET A 1 Q6 25140 IC 74HC574 SOP20 A 3 U10 23 29 25141 74AC32 SOP14 A 2 U37 38 25142 74ACO8 SOP14 A 1 U39 25143 IC 74AC373 SOP20 A 3 U34 36 25144 IC 74AC245 SOP20 A 1 U33 25145 IC 74AC138 SOP16 A 2 U28 30 25146 IC 74HC541 SOP20 A 2 U20 27 25147 IC 74LSO4 SOP14 A 1 U49 26024 IC 2003A TRANS ARRAY SM A 1 U22 27009 MOSFET 2A 80V RFP2N08L POWER 1 2 27013 TRANSISTOR 2N7008RLRA N CH E 1 Q4 28004 VOLT REF 2 5 C 1 U9 28043 VOLT REF 1 2 LT1004 4MV B 1 Q5 28131 IC PBL3771QN STPR MTR DRV SM C 3 U1 3 28133 IC LT1080 SOP18 A 1 U11 29131 TRANSZORB 33V 1 5KE33A B 1 CR3 29137 IC PBM3960QN DUAL 7 BIT D A SM B 3 U6 8 29138 IC AT29C
33. A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 14 Bills of Material ELX50 16 Strip Washer 16 Well ELX50 16 Strip Washer 16 Well rev G As of 11 20 00 Item Description Rev Qty Remarks 16077 WSHR FLT 174X 375X 030 003 A 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070018 ACCESSORIES 384 UNIT B 4 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOLONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOL ONLY 4070527 MANIFOLD 16 WELL ASBY B 4 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072073 PLATE MANIFOLD GLIDE C 1 4072077 STRIP GLIDE A 1 4072079 SPACER GLIDE STRIP B 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOLONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOLONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOLONLY 4072519 TOOL INLET DISTRIBUTION LINE
34. Ball end 3 32 hex wrench 2 Slider Load Spring 4072063 3 Slider Load Plate 4072064 CORRECTIVE ACTION 1 Perform the modifications per the ELx50 Slider Upgrade Kit Instructions 4070004 AW See 4070004 AS Kit Slider Upgrade Page 1 of 1 page 5 3 2 End of procedure Non Mandatory Field Changes Eco 32194 Replace the O ring 49259 on the 4070509 assembly with O Ring 45045 Elx50 Strip Washer Service Manual el L0021 SERVICE BULLITEN DATE 2 SEPTEMBER 1999 CHANGE PRIORITY Discretionary PURPOSE ELx50 piston for the syringe was installed without being polished DISCUSSION ELx50 pistons Part Number 4072060 were installed without the part being polished to reduce friction on the Teflon seal This will cause the Teflon seal to wear prematurely and will eventually leak This Teflon seal could wear after one 1 year of use The polished piston would take years before the Teflon seal wears The serial number range is 144664 144699 145600 145648 Only eighteen 18 unit are affected The ship dates were between June 23 1999 and July 30 1999 CORRECTIVE ACTION For all units returned to Bio Tek Instrument Inc Remove the Syringe and visually verify the piston appears to be polished It should be a high gloss mirror stainless steel finish If the finish appears slightly dull then replace with a polished part PARTS REQUIRED QTY PART DESCR BTI PART 1 Pis
35. T OP TYEE QUOTE cect aig CLER eb dread 4 FAIL c a usa 4 2 1 GOMMECIONS Reo 4 amna 5 3 0 Entering WInmdoOWS u eoe oa se eee Ma Steele 5 4 0 Downloading Base 6 5 0 Downloading Define Assay Configurations 6 6 0 Software Configuration Verification 8 1 9 AutocallDralloty s uo poe eher hee tene Hehe EE ERE Ae CEEE eE BOE 8 7 1 Setting Up and Running 8 8 0 STOP Autocal Verificallblis sese dd eei 9 8 1 Vertical and Horizontal Alignment Check 10 S D CPIGIGICS tui oett aeq ten 10 ONC CURACY ts 11 9 2 Residual NGS z maa ES 14 9 S B rin SUNN U UN IN LL 18 RING III c UN 19 19 0 Label Application usus ete teles 20 Appendix A Bottle and Supplies Maintenance 20 Setting Up Fluidics Test eee faeere Pas eeu a 20 Appendix B Download Computer Setup 20 Append EIFOrGOOBS au a 21 General Emro Sanar
36. The Plate Carrier Check Valves If received by the customer All the rails manifold aspirate and dispense tubes 11 Perform all mechanical Field Change Notices 12 Inspect the up down CAM rubber roller for wear Replace if cracking 13 Verify the up down CAM is not rubbing against the optical sensor as it moves clockwise and counter clockwise 14 Verify the Belt and pully hardware is tight Inspection 15 Replace the top shroud Remove the instrument from the jig stand and install the bottom base pan Setup the instrument to perform a wash 16 Power On the instrument Allow the instrument to initialize At main menu select UTIL TESTS and CHKSUM Record the information on the data sheet 17 Duplicate the customer reported problem if applicable Note your findings on the data sheet PAGE 3 OF 6 4070005 ST REV E SERVICE TEST PROCEDURE 18 19 20 2 22 If required by an Engineering Change Order or requested by the customer update the instrument to latest version of the Basecode and Assay Configuration using the Field Software Download Procedure 8291007 AW Record the information on the data sheet Please inform the customer that the programmed wash routines will be erased when the new Assay Configuration files are loaded NOTE For instruments with Basecode part 4070230 downloaded Please got to step 21 Perform verification of the Vertical and Horizontal Alignment as described in Fina
37. xv wog 335 401 3 P I Wshr 8 Well Pace 2 of 4 NMOHS SV LSZ20 910 ONISN AlddV ATHAO 4070005 AS Final ASBY Stri Elx50 Strip Washer Service Manual el owes 31VOS LON OG I Sv S000Z0b TIIM 8 WdHSVM k dals L 91515 ASV TWN Te eua xencoLwvinewv 9109 L dalS Ses a NN ven NV sns 3 5 031319385 3SIMH3HLO 953 INN Malnoxos o ee NMOHS SV NI 39V1d ova ousvid ND A8SV 43409 8 S30W1d Z WAWININ WOLLOS l d3lS WOU p XOHddV foedus LINN 40 vitis 1080435 ASV Pf VIA 2 dais 335 TIIM 91 Q1O3INVIW NO LINN 40 VINAS ONY Y38WNN ASSV EVN ONIddiHS GNA v 61086 OVE Y OLNI W3HL 30V 1d PNAS S311108 91900 SdVO SAOWSY x xz 6 dais 33s S S190207 44 8 A8 SHINE 56016 HUM 901 3dYL 1SIW S3 11108 40 401 ONY 1H3SNI 338 311108 WNOLLdO ALINHOJNOO JO 9 9150 07 9 84315 ONIdd IHS 0L 9318 U3NNI XOS YANNI Lt LYISNI OL WOVE AVM 3 1 TIY LINN 3H1 1 NMOHS SV XO8 OL NI SNbIOvd
38. 0 O lt misis uu cxlo CID WW I 9 9 lol ZB N 21510010 DJL QF EISS N lt lt lt lt 9 11 Assembly Drawings 5 18 lt Hg A lt oB RE dm Su 8 amp E z 7 50 M z 92 a RE 2 IH 0 ps S dos p 356 Be 55 O scm E E 5 vo 8 Ez L g O g D a ri LO k T ES 5 F7 N 1 5 n a NN 3 ES NN pa lt _ NN N NN L5 N N 0 tu N N ea ro 3 lt a qose 4070511 AS Tubing Set Waste System ASBY Page 1 of 1 4070511 Tubing Set Waste System Assembly rev A As of 11 20 00 Item Description Rev Qty Remarks 4070511 AS TUBING SET WASTE SYSTEM ASBY A 0 DOCUMENT ONLY 4072034 STOPPER 2 LITER BOTTLE B 1 48124 FITTING 3 16 Y CONN PLASTIC C 1 49902 FTG M LUER X 1 4 BARB PP A 1 49904 FTG M LUER X 5 32 BARB PP B 2 49907 FTG 1 4X1 4 ELBOW 90 PP B 2 67262 TUBING SILICON 1 8 ID X 1 4 OD B 0 63 3 75 2X 67468 TUBING SILICON 3 16ID X 3 80D A 4 24 2X 98146 BAG ZIP LOCK MEDIUM 10X12 A 0 Elx50 Strip Washer Servi
39. 05404 0998 Telephone 1 800 242 4685 From within the United States 1 802 655 4044 From outside the United States excluding Europe 33 1 39 30 2276 From within Europe Fax 1 802 655 3399 USA 33 1 39 30 0987 Europe Email LabTAC BioTek com Abbreviations and Symbols The following warning and informational symbols may be found in various locations on the ELx50 Strip Washer Only qualified personnel who recognize shock hazards and are familiar with the safety precautions should repair this instrument o Alternating current Courant alternatif Dreiphasen Wechselstrom Corriente Atterna Corrente alternata Direct current Courant continue Gleichstrom Corriente continua Corrente continua Both direct and alternating current Courant continu et courant alternatif Allstrom Gleich und Wechselstrom Corriente continua y corriente alterna Corrente continua e corrente alternata d Earth ground terminal Borne de terre Erde Bettriebserde Borne de Tierra Terra di funzionamento Protective conductor terminal Borne de terre de protection Schutzleiteranscluss Borne de Tierra de Protecci n Terra di protezione On Supply Marche alimentation Ein Verbindung mit dem Netz Connectado Chiuso Off Supply Arrest alimentation Aus Trennung vom Netz Desconectado Aperto sconnessione dalla rete di alimentazione 1 2 General Information Caution refer to ac
40. 1 of 1 page 5 27 4070522 AS Kit Supply Tube Valve Opto Assembly Page 1 of 1 page 5 29 4070524 AS Syringe Pump ASBY Medtec Page 1 of 1 page 5 30 4070527 AS Manifold 16 Well Assembly Page 1 of 1 page 5 32 Elx50 Strip Washer Service Manual Sa 5 2 Assembly Drawings jo L L SLNAWNMISNI 131 8 40 SUSHIO GASOTOSIG 38 TIVHS NIJYJHL NOLIVINHOJNI AMIGO ONY 86 6 L L HIINS 8 A8 NYG AN3SNOO 553 4 3 LNOHLIM NOLIVINHOJNI 3H1 YON LI WILNACISNOD SNIV LNOO LNSWNOOG 5 JANLIYN IS g SY3HLO 03S 0 GALYANA oua 326 SSHIBNONV ON SINJWNYLSNI Y31018 lt YANS Lm YONG 3015 SIH L OLOHd 98660 VOVSO DISOONIA 866 Vd GNVIHSIH SIN3IWDHISNI 99 31N9S NOLI OUA STONY GAIL SNIMVSO 138dH31NI LON OQ 3NS3HQV HSINI3 WielalviW 1 SIG XX SIQL X OL NOWONGOUd S3HONI NI 38V SNOISN3WIQ NY 3 38 OL SYOIOD 931512345 3SIWI3HIO 5531 d3dvd 3 LIHM 02 Lb X 748 OLNO Q3ldO9010Hd SI GNVH 1531 SHL 1VH1 HONS YAldOOOLOHd JHL NO 9NIMVSG 39V Id MYOMLYV H31SVIN SV 9NIMVSO SIHL 3SN 31ON 86 vc c NOLLOnIGO3d 3SV3 13H diva NOLLdIH2S3Q V A38 000 07 pauinbas oN quiesse e1 Jaye uonounj ejeptleA Rasy 19 09 JosoJey pue pjojlue J
41. 3 3 o g V 4070518 AS Seal and Cylinder ASBY Page 1 of 1 5 27 Elx50 Strip Washer Service Manual 4070518 Seal amp Cylinder Assembly rev A As of 11 20 00 Item Description Rev Qty Remarks 4070518 AS SEAL AND CYLINDER ASBY B 0 DOCUMENT ONLY 4072045 SYRINGE PUMP CAP MACHINED C 1 4072511 JIG SEAL ASBY A 0 TOOL ONLY 49909 SEAL ENERGIZED 875X1 12 PE A 1 66033 LOCTITE INSTANT GEL C 0 AIR 5 28 Assembly Drawings L L L avos i SV ccSOLOv g ONIMWG 3215 A8SV NOILdO 3ATVA 1 AlddNs 11 aul VILN3QIHNOO SNIVLNO9 1 SIHL s3903 dHVHS JAOW3H Suns Tv HSINI 31 95 LON OG VIV A8 ava 023 NOlLdiios3a 548 16 61 58 zezez 3SV3138 848 2616 6 11862 z 31ON Gav Sag 86 9 2 vzOE Ova 313730 Z 310N OHO 9 8660 r0rS0 1 IMSOONIM 866 XO8 Med ONVTH IH xl o 16 6185 Y NI 26 82 7 4 3 34 5 S007500C OLO FXX TOL SLO X S3ONVH3 TOL NOILONGOYd S3HONI NI SNOISN3WIO TIV diva 5 G3I4IO3dS ASIMYSHLO SSJINN Page 1 of 1 ly Tube Valve Opto Assembl Remarks IN BAG IN BAG DOCUMENT ONLY IN BAG IN BAG IN BAG
42. 384 well at the same time yet it allows for overfill washing of the smaller 384 wells The dispense tubes are held above the plate when the dispense manifold reaches a mechanical stop while the aspirate tubes are able to continue descending into the wells MANIFOLD _ MCROPLATE i x y Y N g 1 N N NN P Sd xl d N N N N N N M KA Kay Neo Nee Ne Nec eec ses ce NN GUSTOS Y GCUOUSIOOUN C RIK X28757 X X25757 X X25252 KILL KPI GP RU X F DISPENSE UD CARRIER HD HEIGHT z zEzzzz zzzzz LILI EN E N N N 4 d N NA TEXAS x ee 09 K XRD KARL K KARI KK RIL KK RIL EX USC 5 mm aan HEIGHT E c Single manifold system of the 8 and 12 channel manifolds LEE Dispense manifold UU mE b Tubes lt positioned for la H overfill washing v Z L OLII III 77 2 Aspirate manifold Tubes JE H BEBE positioned for J Z aspiration xd zzzzzzzzzzzzzzzzzzzzzzzzzzz2ZZzz2 p LL L Patented dual manifold system of the 16 channel manifold 2 2 Functional Description The following table shows the washing capabilities of each manifold Manifold Washing Capabilities 8 channel 8 well strips 96 well plates 8s channel 8 well strips 96 well plates
43. 98 operating system as needed Note The PC in use as a download tool will need its windows environment configured one time Once properly setup the system should work as described in the following procedure See set up appendix at the end of this procedure PAGE 5 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 4 0 Downloading Base Code Insert the 4070201 FW Operating Software disk into the A drive of the PC Use the mouse and double click on the ELx50 Utilities Icon if the ELx50 utilities dialog box is not already open Double Click on the ELx50 Code Download Icon Swiss Army knife in the dialog box On the reader display Download In Progress will appear The following will appear the computer screen Represents any number Download Utility 7330201 Version Downloading elx50 bin to washer File size this number may vary with version amp model number Bytes sent this will count up during download Checksum this will appear at completion of download Download Successful this will appear at completion of download The UUT will reset at this point and may display an error OBFF because there is no assay configuration loaded yet This may be variable depending on what has been done to the instrument Press the Stop key to stop the beeping and wait for the main menu to appear 30 sec then press the shift and 2 hidden key between Main menu and Previous Screen keys This may b
44. AS Check off items below as completed Item Results Shoulder screws on carrier rail and manifold bracket rail are tight _ Pass Fail Four set screws in shaft collar of manifold bracket rail are tight with loctite applied Completed 4072503 jig for cam assembly is tight against post and slides freely with little or no gap on jig _ Pass Fail surface Pass Fail Ensure they are installed correctly high and low in the correct sockets Pass Fail Completed Completed Tubing clip shell plug tube aspirate stylus and tube dispense stylus on cover verify stylus per _ Completed BOM Using a 19396 screw verify the 2 threaded holes for the aerosol cover do not have any paint in them Completed Metal strip installed in the carrier EIx50 16 units only Completed By Date DOWNLOAD TESTING Refer to 4070020 TP TEST PROCEDURE Para Item Results Limits Record Base Code Revision Version loaded Base Code Rev Ver Record Assay Def Revision Version Loaded Assay Def Rev Ver Verify Correct Firmware Installed Per BOM Completed PAGE 3 OF 4 4070020 DS REV C Final Test Data Sheet EIx50 16 Certificate WO Unit Para Item Results Limits 5 UUT Configured For Correct Manifold 8 12 or 16 8 Check Manifold s Pass Fail 8 12 16 8 Auto Cal Performed _ Completed 79 2 Carrier Rests On Feet With No Rocking _ Pass Fail 3 Verify Aspirate Tubes Are Less Than 023 Above J
45. Costar Flat Bottomed 96 Well Plates BTI Jig Manifold Alignment Jig Microplate Stop Align with pin removed Jig Stand Asby Jig Autocal Verify Jig Manifold Calibration Jig 16 Channel Manifold Asby Jig Cam Alignment Jig Carrier Flatness 0 375 001 high spacer 3 8 x 3 8 lathe bit Stylus Tube Aspirate Stylus Tube Dispense Strip Washer Final Test Procedure WINWORD PAGE 1 OF 6 Part Number 4071000 4071008 61062 75011 4070515 48140 4070510 4070511 4070005 IP 98102 4072500 7342500 4072501 4072526 4072505 4072524 4072522 4072521 4072042 4072043 4070005 TP 4070005 ST REV E SERVICE TEST PROCEDURE Precision scale with minimum 0 01 resolution and 120 gram capacity DI with 0 1 Tween 20 solution FD amp C 1 Blue Dye Warner Jenkinson Part 05601 1105 50 200 ul pipette with tips Tool Inlet Distribution Line 4072519 Supply Tube Valve Option Asby 4070522 Null Modem Serial Cable 75053 Test Station MQX200 QC Assays 4070600 Parallel Cable 71072 Parallel printer emulating Epson mode Field Software Download Procedure 8291007 AW PC 386 min with 1 44mb 3 5 floppy drive 1 COM port minimum mouse and 4 MB RAM Windows 3 1 or Windows 95 installed on above PC Download Utility 8290206 FW Basecode and assay configuration firmware see BOM for specific model requirements Instructions 1 Decontaminate washer as described in ELx50 Operator s Guide 4071000 If the customer complain
46. Final ASBY Strip Wshr 8 Well Page 3 of 4 enne nnne 5 6 4070005 AS Final ASBY Strip Wshr 8 Well Page 4 of 4 sse ener 5 7 4070507 AS Mechanical ASBY Page 1 of 3 eene ener nnne nnne nennen 5 9 4070507 AS Mechanical ASBY Page 2 Of 3 eene enne enne nnne rne 5 10 4070507 AS Mechanical ASBY Page 3 Of 3 nennen enne ener ener enne nennen 5 11 4070508 AS Carrier ASBY Page 1 of luu aha anku A ennt nennen nnne trennt enne E 5 14 4070509 AS Lift Motor Cam ASBY Page 1 of ener enne nnne 5 16 4070510 AS Tubing Set Supply System Assembly Page 1 of 1 sse 5 18 4070511 AS Tubing Set Waste System ASBY Page 1 of nennen 5 19 4070513 AS Manifold ASBY 12 Well Page 1 of 1 enne 5 20 4070515 AS Bottle Supply ASBY Page 1 of 1 enne enne enne 5 22 4070516 AS Syringe Pump ASBY Page 1 of 1 ener ener nnne enini 5 23 4070517 AS Aerosol Cover ASBY Page 1 of 1 ener nre nennen een nennen 5 25 4070518 AS Seal and Cylinder ASBY Page 1 of 1 nennen nennen enne nnns 5 27 4070522 AS Kit Supply Tube Valve Opto Assembly Page 1 of 1 5 29 4070524 AS Syringe Pump ASBY Medtec Page 1 of l
47. G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070512 MANIFOLD ASBY 8 WELL B 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 4 Bills of Material ELX50 8V Strip Washer 8 Well w Valves ELX50 8V Strip Washer 8 Well w Valves rev R As of 11 20 00
48. G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070513 MANIFOLD ASBY 12 WELL B 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 12 Bills of Material ELX50 12V Strip Washer 12 Well w Valves ELX50 12V Strip Washer 12 Well w Valves rev R As of 11 20 00
49. IN BAG IN BAG 1 5 4 3X 5 25 8 25 11 11 25 24 3X IN BAG Qty 3 3 0 1 2 2 4 4 10 1 Rev 5 29 xe 0 L O00 YZ c9249 x9 EH 59 Kam L066v 5 S V OVE 2115 1 OLNI WO8 SLN3NOdNOO 11 SONILLIJ 3H1 JO 8 IHL OLNO ATIN ONISAL HSNd ONMENASSY N3HM 4 OL 10H09 v ISN Yad S38n1 A1ddns ang 7 SALON 4070522 AS Kit Su 4070522 Kit Supply Tube Valve Opto Assembly rev C As of 11 20 00 KIT SUPPLY TUBE VLVE OPT ASBY JUMPER FOR BERG HEDRS FITTING 3 16 Y CONN PLASTIC CLIP WIRE PVC SELF ADHESIVE BUSHING SNAP 250ID FTG F LUER X 5 32 BARB PP FTG M LUER X 5 32 BARB PP TUBING SILICON 1 8 ID X 1 4 OD BAG ZIP LOCK MEDIUM 10X12 CABLE SOLENOID ASBY Description NUT KEPS 8 32 Elx50 Strip Washer Service Manual Item 15011 4070504 4070522 AS 42113 48124 49539 49893 49901 49904 67262 98146 L L anvos 48 awa 3903 suuna Tv j lawuswvaue anona 3 IVOS LON OG sas ewe orze NOUOGONdOLSSVETAN V V SV vcS040v 2 NOLANA STONY GEH 9NIMYHO azis IM OALGAW ASV M SQVAYHL 90099 3111201 Alddv 39NIHAS AVIS OAQNY 7
50. Make sure that the check valve is in place and that the flow arrow is pointing toward the manifold When testing 16 channel manifolds it will be necessary to run the reader test station from a remote computer This PC will have Windows 9X operating system Excel and KC jr software loaded Connect the serial port of the computer to the serial port of the uQuant Power on the computer and open the KCjr program Open the EXCEL spreadsheet program and the files ACCURACY XLT and RESIDUAL XLT From the Main Menu select RUN PRIME and press the OPTION key if necessary until NEW BUFFER PRIME is displayed Then press the ENTER key to select this program If priming empty supply lines then repeat the PAGE 11 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER priming steps twice For washers with valves press the ENTER key again to select valve A Press the ENTER key or the START key to run NEW BUFFER PRIME For washers with valves repeat for valves B and C This will prime the washer with the 300 ul Solution When the program is complete press the MAIN MENU key TESTING 10 Place a Costar flat bottomed plate on the balance and tare the balance Use a 96 well plate for testing 8 and 12 channel manifolds and a 384 well plate for testing 16 channel manifolds Load the plate onto the carrier of the UUT making sure that the orientation of the plate in the carrier matches that of the manifold in the wa
51. NOTE TURN UNIT OFF AFTER AUTOCAL TO ENSURE UNIT HOMES AT NEXT POWER UP BEFORE FLUIDICS TEST 8 0 Autocal Verification 1 Verify that the carrier has the same serial number as the instrument 2 Verify that the carrier rests on both adjustable feet and on the rail without rocking 3 Install Autocal Verify Jig 4072526 into the carrier The jig must be positioned correctly in the carrier One corner of the jig is labeled 12 CH with an arrow pointing to the corner Another corner of the jig is labeled 8 or 16 CH with an arrow pointing to the corner Choose the appropriate corner for the manifold being tested and align that corner toward the front and against the right edge of the carrier 4 Place the 8 12 or 16 channel manifold on the manifold bracket 5 Configure the UUT for an 8 12 or 16 channel manifold whichever is appropriate From the Main Menu use the soft keys and select UTIL SETUP MANIFLD and press the soft key to select an 8 12 or 16 channel manifold Press the Main Menu key to return to the Main Menu PAGE 9 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 8 1 10 Vertical and Horizontal Alignment Check Use the soft keys and select UTIL TESTS and press the Shift Key and the 1 hidden key simultaneously the 1 hidden key is located between the clear and enter keys Use the soft keys and press AUTOCAL VERIFY and then press the ENTER key The carrier will slowly move into t
52. PSA B 1 49918 TUBING CLIP 1 4DIA SELF ADHESV A 1 7342500 JIG MICROPLATE STOP ALIGN E 0 TOOL ONLY 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 91093 LABEL 4 X72YDS CLEAR TAPE A 0 A R 5 8 Assembly Drawings Beales 278 3 LON OG zz E SESS Oe NUI 5 205020 su EENE M mar aeuo EE L 7 E OE 8 visa V9 3ovsuns como 5 NOCT E ozozee2 tumse as wea SSHON NI JYV SNOISNSWIQ 7 ozozeez EZ 3univwols 3119395 3SIME3HIO SST INN c dals NMOHS SV nau iaans NMOHS SV OZOZEEZ OLNO 0202 SLVLOY 00 30V ld z r xL 98161 HLIM YIL OY TS 3H1 SAOWSY OL 0704 4315 NMOHS Z A1NO 1 9 0 0 v vlad 335 2002 07 1 8 v xe 9 1 130 33S 91091 0 4915 z 133HS WLI erie 3d SIN3AGMInO3H 2802107 SNIOVdS 3H1 NIVL8O OL NMOHS SV AHVSS3O3N SV H3HSVM 8318vo ge v1lvi3a 33s 99041 QNOO3S V TIVLSNI TA z 4315 335 98041 xz 081 2108 g anavo lt 862 HLIM 9690212 33s Ganaans 020 9 8
53. SOCK CAP 4 40X3 8 SS B 2 19143 SCR SOCK CAP 8 32X1 2 SS A 4 19397 SCR SELF SEAL 10 32 75 A 1 19401 STANDOFF M F HEX 4 40X9 16 SS A 2 4070506 CABLE SYRINGE PUMP ASBY D 1 CABLE B 4070516 AS SYRINGE PUMP ASBY B 0 DOCUMENT ONLY 4070518 SEAL AND CYLINDER ASBY A 1 4072023 MOUNTING BRACKET SYRINGE PUMP 1 4072025 NUT LEAD SCREW B 1 4072027 MOUNT SYRINGE PUMP PISTON D 1 4072060 PISTON SYRINGE PUMP POLISHED A 1 4072506 JIG GLUE SYRINGE PUMP B 0 TOOL ONLY 66006 LOCTITE 242 BLUE A 0 AIR 66039 GREASE LINEAR WAY A 0 A R 5 24 Assembly Drawings L 30 L iie vie aos E Q 3903 syna Tv AWwos LON 28 oo 4 Nolidiossd duvHS SAOWSY saa 2519 Nouonaotd 3sv3 as g SV ZLSOZOV c NOILOSPORd GIL saa zesz t s800 S31ON 310N 313730 SLNSWNYLSNI 1 40 ji 326 SH3HLO AS8SV Sti3H1O OL 038079810 38 TIVHS G3NIVINOO NOLINIHO NI HON sid TOSOYAV VITOR ONY S00 FXXX SNIVINOD 1N3WnOOQ SIHL 9 SOW Jh m OLO0TXX eX ZFX YYIN NY SLOFX 2860 050 A DISOONIM araa S3ONVH3T01 NOILONGOYd 866 ONVIHOIH 18 66 HiWSS NI YW SNOISNINIG TV SYNLWNOIS G3I4IO3dS ASIMYSHLO SSA INN
54. SY 1S0d 9002207 2 ANNOYY TIVLSNI xr 60 8616 6 8006205 8106205 180d Way IN3ANS HI E 902407 81020 xz 3H VS VH LNSWNOMV IVLNOZINOH sod 3sve DL xz LN3WN9STTV TWOLLYSA v902 0v r I H 9 4 3 9 g v echanical ASBY Page 2 of 3 4070507 AS Assembly Drawings 5 10 20 123i 8 9 31 LON OG I sSv 205070r onoma A8SV IVOINVHO3IN HSIN vaiva T ne Tan 500 HO 4 6 XZEXIOLUVIN NY 60 S3HONINI SNOISNGINIO TIV SHRUVNOIS 091319395 3SIMH3HIO SS3TND IVOINVHO3M IHL HLIM NIVW3H LSNW S3HSVM SIHL HO3 O31Sn rav N338 SVH IHL ILON Q3HOVLLV LNOHLIM 1 NMO 51 TIVA CINOHS 130 VH 401 d31S 41 O 9 D QVI SLOTS OLNI 5309 OSV 9 51045 LSNIVOV 1V14 SSHONOL TILLS WALI 3 9 301 4315 86161 3 18 3SSV3N ONY 31100 T3AOWSM INIL V LW INO SHYTIOO JO NOI1V2O1 HOS 8 4415 343 3qIS ANY 1431 3HL NO S NIHSNA OlLSV 1d ASNIVOW 1 G3HSfld 3HA3HL OS SHY TIOO INSINLSPrQv N31HOIL S ALON 33S LNSWLSNPV OLNI SM3HOS 13S GSNYV LOTS NI SI 1804 Sif N3HM OS OL 1 YO 1331 13 0 V8 3 301S 5 11
55. Sag 80ZZe usd OHO 8 azis AN3SNOO SS3HdX3 LNOHLIM L AHSV IIHM Bk m Q10JINVIA EO RN o eres X C X SLO X un papa S3HONI NI dv SNOISNGNIG r d31S NON 5 aiva 3HnivN9IS 031512395 ASIMYSHLO SS31Nn 822 OO d31S NI 38001 1331 SMAUOS S N31H9IL 7902 0v OL T3TIV Vd S LI OS 5 02 0 1Snrav 74121 SMANOS 4315 ONILNNONW Sif IHL LH3SNI SNId H3TIVINS Z FHL OLNO 2906105 JO WO LLOS SHI NI 7 36H 8 LOTS SHL NONY SNIS3MOT 3 1 4315335 SNId 3 1 IHL NO d31S 3 1d e 2 T T LE A8SV Ne HO 9191 rhs 9 0 6106109 NALHOLL H39NIJ LOLZL das 335 06105 9 40 LHYd 490240 SH3HSVM 66041 AS 9 MIYOS 2161 d31S 2 uu 890240v JO 5 d31S 90 20 se ud I Ss es xz a 98v6y xz 10240v 6466L 8 LNO LND WYO4 39v 1d ATIQ33HVO 9 OVE QNVH 11086 NI 3 ld A8 v d3lS 335 8 6 0667 1 IHL OL v6299 3111201 e 98v6y V 6 8066 3 Alawassv VA OL 3 ATIVNS2LX3 ANY ATIVNH3LNI HLOS 3384 LSNA 2902207 38 LSNW ATHN3SSV A 18W3SSV OL HOlsd MHAO ION OG AY OHOO Iv HLIM 9 15 HSYM
56. When the verification is performed on this axis the software compares the number of steps observed at its present position to the actual number of steps required to move the axis back to the sensor home position At the completion of a dispense aspirate or wash program the carrier position is verified There are two scenarios 1 Ifthe carrier is on the sensor or the observed motor position is less than 3 full steps from the sensor then the carrier is moved away from the sensor 6 full steps The carrier is then moved toward the sensor until the sensor is marked If the observed motor position is outside the range of 3 full steps to plus 3 full steps inclusive then an error is generated 2 Otherwise the carrier is moved toward the sensor until the sensor is marked If the observed motor position is outside the range of 3 full steps to plus 3 full steps inclusive then an error is generated Error Codes Non fatal errors 0x1900 Ox 1A00 0x1B00 SYRINGE FMEA ERR 0x1C00 1 sensor not found at start of syringe aspirate stroke 2 sensor not open at start of dispense stroke 3 syringe aspirate stroke failure 4 sensor verify after last syringe stroke in program failed 5 sensor clear from home position too many steps MANIFOLD POS ERR 0 1000 could not calculate manifold position for offset provided AUTOCAL CHECKSUM ERR 0x1E00 3 2 Error Codes Motor Identification Manifold O Fatal errors All
57. diskette In drive a other floppy drive designators may be avallable at this screen It is recommended that the configuration files be loaded from a floppy drive wINSTALL Message Ready to load diskettes Press to continue This may take a few minutes The Download Utility files are being loaded In most cases there is only one Download Utility disk When the installation of the Utility is complete an ICON similar to the icon below will be seen in Windows 3 1 or Windows 95 Remove the Download Utility diskette from the computer s floppy drive PAGE 12 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE 3 3 Insert the CONFIGURATION diskette into the floppy drive Double click on the Download Utility Icon The floppy drive should become active while the application is initializing The following screen or similar should appear on the computer R EL ICC I lt IRKV HVIEII T IIZI I II IHl N ET I IIIIII IIIIAII IIIAIIAIIAIITNII0IWAI T Download Target Dialog Select ELx800 808 Reader for ELx800 ELx808 uQuant and PowerWave Readers Select ELx50 Washer for the Elx50 Select ELx405 Washer for the ELx405 After Selecting click OK A screen similar to the one below will appear PAGE 13 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE Download Utility J ET n e Version Language Download Language Assays
58. instrument display does not read Start Assay Download the following message window may appear Define Assay Download AN Unable to start the download sequence Error Message Receive Error Check that the reader is ready for download and that the proper cable is being used v Check that the cable is connected between the computer and the SERIAL port of the instrument It may be necessary to check change the configuration of the serial port in the Download Utility under Setup Serial Port If the instrument does NOT display Start Assay Download press the Shift and Hidden Key 2 simultaneously Hidden Key 2 is between the Main Menu and Previous Screen keys The instrument should display START ASSAY DOWNLOAD YES NO Readers START PROGRAM DOWNLOAD YES NO Washers Choose YES then double click on the DOWNLOAD button in the Download Utility start the process again After the configuration download is complete the Instrument will re initialize and return to the Main Menu Check that the current software is loaded onto the instrument by pressing UTIL TEST CHKSUM The instrument display will scroll through the seven digit part numbers and versions of both the Basecode and Configuration part numbers Check them against the list at the end of this procedure The software update is complete ELx50 16 Users ELx50 16 software defaults to an 8 channel manifold configuration at download When the download is compl
59. of these items are system configuration values which can be changed and loaded via extensions without a rebuild of the software System Configuration Vacuum Pump Startup Delay o 0 10 seconds Trough Dispense Height 96 12180 Trough Aspirate Height 30 12180 0 0 Trough Clearance Height 146 12 180 1 1 Crosswise Clearance Height 64 12180 Plate Clearance 146 OP Valve Open Delay 0 065000 milliseconds Valve Close Delay 0 0 65000 milliseconds 16 steps full step resolution See the Operation section of the operator s manual for current information Elx50 Strip Washer Service Manual 3 3 3 4 Error Codes Section 4 Bulletins This section contains Mandatory and recommended field change notices bulletins Mandatory Field changes FCN L0015 SERVICE BULLITEN DATE 31 MAR 98 ENGINEER TOM GRAVELIN CHANGE PRIORITY Mandatory PURPOSE To reduce the plate carrier noise when in motion This change should be performed on ELx50 units with serial numbers below 137288 Rev 21 Apr 99 DISCUSSION When the plate carrier was in motion the plate carrier would vibrate against the carrier slide A load spring is to be added to reduce the vibration EQUIPMENT REQUIRED 1
60. or 1x12 RESIDUAL test is displayed Press the ENTER key to select the appropriate program 11 Enter the Serial Number of the instrument being tested for the PLATE ID and press the ENTER key 12 Enter the Weight including the decimal point and press the ENTER key Note if the weight was 01 or less enter 01 13 Press the ENTER key to start the read The reader will read the plate calculate the results and print the results 14 To print a second copy of the report on the reader key pad press the REPORT soft key the RESULT soft key and the OPTION key until the file PAGE 16 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER to print is displayed then press the ENTER key NOTE Follow steps 15 25 for 16 channel washers 15 Open Excel selecting File test 384 16 channel residual xlt Minimize Excel and Open KCjr In KCjr program select Protocol Open protocol 384 630450 Refer to Appendix E to create this protocol if it isn t present 16 Click on Read Plate read plate at dialog and Ok The uQuant will read the plate and the absorbence data will be displayed 17 Use the mouse and select the Transformations Tab then use the arrow keys of the computer to move to well A1 of the data displayed in KC jr 18 Press the Shift Key and simultaneously use the arrow key to move the cursor from A1 down and over to highlight A1 P6 The wells from A1 P6 Should now appear highlighted 19 Press the CTL Key and simu
61. oy es os zeoo ids OL 99 19313S 900VbL ovi ea 529 4070400 SC Main PCB Page 3 of 6 Schematics 6 8 1noisssu OS 00r020r 9 30 133HS awos so Em 82d S IVAOBddV 8660 90990 LA MISOONIM 866 Nd ONI SLNAWNUYLSNI L 35 E A4 soni ai o ozv oa lir el 95 sa L el sa er T S i x ea Is sr E zo za oooroozeezi f 5 kD Z 50 why go aoi oa 00 oa 1 z vCXZ 3001 y 19 Wnnov ur ad 5 5 T T T n lt n D 9 vi Ho 2478 z ey T Te woz ds 4
62. receptacle with a functional ground Warning Internal Voltage Always turn off the power switch and unplug the power cord before cleaning the washer s outer surface Warning Liquids Avoid spilling liquids on the washer fluid seepage into internal components creates a potential shock hazard Wipe up all spills immediately Do not operate the instrument if internal components have been exposed to fluid Warning Potential Biohazards Some assays or specimens may pose a biohazard Adequate safety precautions should be taken as outlined in the assay s package insert Always wear safety glasses and appropriate protective equipment such as chemically resistant rubber gloves and apron Warning Software The washer operator must follow the manufacturer s assay package insert when modifying software parameters and establishing wash methods using the washer s on board software Precautions The following precautions are provided to help avoid damage to the instrument Caution Service The washer should be serviced by Bio Tek authorized service personnel Only qualified technical personnel should perform troubleshooting and service procedures on internal components Caution Environmental Conditions Do not expose the instrument to temperature extremes For proper operation ambient temperatures should remain between 15 40 C Performance may be adversely affected 1f temperatures fluctuate above or below this range Storage temperature limits ar
63. which is closest to the jig surface and that which is furthest from the jig surface is less than 010 inches Press the ENTER key The manifold will lift and the carrier will move further away from home and the manifold will lower into holes in the jig Verify that the manifold aspirate tubes have moved into the holes without catching the edge of the holes and lifting the manifold Press the ENTER key The manifold and carrier will return to home Remove the jig from the carrier and press the Main Menu key Mark the data sheet to indicate that the UUT has met all of the above criteria Repeat of sections 8 0 and 8 1 for a second or third manifold as necessary Initial the routing to indicate that the UUT has met all of the above criteria PAGE 2 OF 2 CONFIDENTIAL Bio Tek Instruments Inc Model ELx50 Strip Washer Procedure 4070020 TP Title Final Test Procedure ELx50 Strip Washer Rev Description of changes ECO Date A Release To Production 32594 07 21 99 B PG 9 Modify The Autocal Verification 32708 08 17 99 C Chg section 3 0 to Make Generic Highlight 33701 06 27 00 In Para 7 1 8 0 8 1 Order of Accuracy Changed Add to Residual Test Chg Para 1 In Clean Up Section Update Index D Add To Para 7 1 Step 5 Remove from 33806 08 16 00 Para 9 1 Step 20 Chg Para 9 4 Step 2 Table of Contents 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Equipment and Materials 3
64. 00 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 99125 LABEL 1 X1 2 CONTINOUS C 1 Elx50 Strip Washer Service Manual ELX50 8GVS Strip Washer 8 Well Generic w Valves Short Dispense ELX50 8GVS Strip Washer 8 Well Generic w Valves Short Dispense rev R As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070515 BOTTLE SUPPLY ASBY B 1 407051
65. 010 12TC FMEM 128K SM B 2 U44 45 29139 IC AM29F040 120 512K FMEM SM B 2 U46 47 29140 IC 128KX8 STATIC RAM TSOP 8X20 2 042 43 35009 TRIMPOT 20K 25T A 1 RT1 37002 RESNET 10K OHM 5R 6P SIP B 4 RN13 16 37007 RESNET 180 OHM 5R 10P SIP B 3 RN3 5 37046 RESNET 10K SIP 10PIN 9RES 5 A 6 RN6 8 10 12 38005 RES 1 00K OHM 1 SIZE 1206 D 7 R11 16 21 38026 RES 475 OHM SIZE 1206 A 1 R43 38067 RES 65 OHM 1W 1 B 6 R3 8 38086 RES 29 4K 1 1 8W 1206 B 1 R37 38087 RES 10 0K 1 1 8W 1206 A 1 R38 38088 RES 30 OHM 5 1 8W 1206 B 3 R40 42 38089 RES 3 01K OHM 1 1 8W 1206 B 1 R32 38090 RES 2 0K OHM 1 1 8 1206 A 1 R36 38091 RES 3 32K OHM 1 1 8W 1206 A 1 R22 38093 RES 15 0K 1 1 8W 1206 B 3 R18 20 38094 RES 2 49M 1 SIZE 1206 A 1 R39 4070200 FIRMWARE ASBY ELX50 V1 02 A 1 U40 HIGH U41 LOW 4070400 AS MAIN PCB ASBY D 0 DOCUMENT ONLY 4070400 SC MAIN PCB SCHEMATIC D 0 DOCUMENT ONLY 4070400 TP MAIN PCB ASBY TEST PROCEDURE A 0 DOCUMENT ONLY 4071400 MAIN PCB D 1 42112 HEDR 4 PIN DUAL B 2 P1 P4 6 4 Schematics Item Description Rev Qty Remarks 42150 HEDR 12 P 100 RTANG BRKS B 1 6 42225 HEDR 3 P 156 LOK A 1 42307 SWAGE CONN 5 32 RND 1 41 B 4 SWAGED INTO PCB 42310 HEDR 14PIN FOR LCD MOUNTING A 1 J9 MOUNTED ON 42311 HEDR 14PIN 1X 1 FOR LCD BL SEIS 42319 HEDR 4 P 100 PCB MOUNT GOLD A 2 23 42324 HEDR 5 P 100 PCB MOUNT GOLD A 2 J14 15 4246
66. 0505 CABLE VACUUM PUMP POWER ASBY B 1 CABLE D 4070507 AS MECHANICAL ASBY J 0 DOCUMENT ONLY 4070508 CARRIER ASBY A 1 4070509 LIFT MOTOR CAM ASBY B 1 4070516 SYRINGE PUMP ASBY B 1 4071004 LABEL REAR A 1 4071005 LABEL POWER IN SERIAL A 1 4072002 BASE PAN E 1 4072004 MOUNTING BRACKET MANIFOLD D 1 4072005 SHAFT MICROPLATE CARRIER F 1 4072008 SHAFT PIVOT E 1 4072013 COLLAR WASHER SHAFT B 2 4072018 SLIDER MOLDED B 1 4072032 STANDOFF MANIFOLD BRACKET F 5 4072033 STRIP FOAM A 1 4072063 SLIDER LOAD SPRING A 1 4072064 SLIDER LOAD PLATE A 1 4072082 PARTITION BASE A 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072502 JIG TEFLON FOAM STRIP ALIGN B 0 TOOL ONLY 4072503 JIG LIFTING CAM ALIGNMENT B 0 TOOL ONLY 41049 BELT TIMING 260T 1 4W D B 1 42058 TIE WRAP 3 A 3 42210 SCREWLOCK FM 25P SUB D A 1 45056 FOOT RUBR 1 0620DX27 32H3 16ID A 2 5 12 Assembly Drawings Item Description Rev Qty Remarks 49128 SPCR FF 6 32X 25L HEXAL B 4 49894 BEARING SNAPIN 3121D TEP642 W A 2 49895 STDOFF FF 4 40 1 125L AL HEX B 2 49898 GROMMET SPLIT PLASTIC 625 ID B 1 49899 GROMMET SPLIT PLASTIC 500 ID B 2 49902 FTG M LUER X 1 4 BARB PP A 1 49905 FTG F LUER X 1 4 BARB PP A 1 66006 LOCTITE 242 BLUE A 0 A R 66023 LOCTITE PRIMER T B 0 A R 66039 GREASE LINEAR WAY A 0 66290 TAPE UHMW 030X 5 ADHES BACK A 1 67468 TUBING SILIC
67. 100 ul of DI Tween 20 to the wells containing the residual dye solution The ELx50 Test Station with a 16 channel manifold may be used rather than the hand pipette method ELX50 300 ul TEST STATION SETUP 1 Select test manifold 8 12 or 16 channel PAGE 15 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Depress soft key Util Setup Manifold select 8 12 or 16 and return to the main menu Depress the soft key Disp then options select Disp 300 for 8 or 12 ch or Accur QC test384 16 channel Select 6 strips for 8 or 16 ch units Select 4 strips for 12 ch units Depress soft key Enter Main Menu Run Prime Option key to select New Buffer Prime then enter twice Weigh tray place in carrier press Run Disp Enter twice 9 Place the plate in the carrier of the wQuant reader test station with the strips containing the fluid to left side of the carrier or to the back of the carrier depending on which manifold is being tested Residual Test Limits For 8 and 12 channel manifold testing passes unless Residual Test Failure is displayed in the comments section For 16 channel manifolds Average residual 1 0 mg must say PASS and Maximum residual 1 5 mg must say PASS NOTE Follow steps 10 14 for 8 and 12 channel washers Go to step 15 for 16 channel washers 10 If testing an 8 or 12 channel manifold then use the soft keys to select READ and then press the OPTION key until 1x8 RESIDUAL
68. 11 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 6 Bills of Material ELX50 8S Strip Washer 8 Well Short Dispense ELX50 8S Strip Washer 8 Well Short Dispense rev P As of 11 20 00 Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOLONLY 4070519 MANF 8 WELL SHORT DISP ASBY A 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOLONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOLONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOLONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0
69. 1BOO Carrier offset position is out of range for present manifold SYRINGE_FMEA ERR 1C01 Sensor not found at start of syringe aspirate stroke SYRINGE ERR 1002 Sensor not open at start of dispense stroke SYRINGE ERR 10053 Syringe aspirate stroke failure SYRINGE ERR 1C04 Sensor verify after last syringe stroke in program failed SYRINGE FMEA ERR 1C05 Syringe FMEA failure MANIFOLD_POS_ERR 1D00 Could not calculate manifold position for offset provided AUTOCAL CHECKSUM ERR 1E00 Autocal checksum error PAGE 22 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Fatal Errors These errors indicate fatal conditions that require immediate attention If a fatal error is displayed contact Bio Tek s Technical Assistance Center for further instructions Error Cause Code TCB NOT AVAIL ERR A100 Task control block not available READ NOT AVAIL ERR A200 Reader function already in use NOT AVAIL ERR A300 Device not available Devicenotavaiable Appendix D Clear Assays 1 Place any assay disk in drive AX double click on 800 Assay Definition Icon 2 Click on ELx50 strip washer Click on OK 3 Plug serial cable into ELx50 Turn on ELx50 push SHIFT key and hidden key between Main Menu and Previous Screen to make unit ready for download Press YES on ELx50 4 Click on SETUP click on Clear Wash Programs Click on the box to the left of remain in download mode to
70. 20 TP REV 4070005 IP REV Note The Following Steps Correspond To The Order Of Steps On The 4070005 ST Document Step Item Results Limits Record accessories received Carrier Aspirate Tubing set Bottles Dispense Tubing set Manifold Aerosol Cover Stylus Kit Power supply Power Cord Inspect the Instrument for loose hardware and proper tubing Completed v placement VA 5 to Ground 5 525 4 475 VDC EPROM information Part Version Rev Power Supply Checks VCC Across C8 and Ground 27 5 23 5 VDC Remove the top shroud Completed v Page 3 of 5 4070005 SD REV B SVCE TEST DATA SHEET Return Authorization Syringe Cap Inspection Completed v Comments Manifold Gasket Inspection Completed v Comments Cleaning of Completed v Plate Carrier Comments Check Valves Plate Carrier Rail v Completed v Comments Completed v Comments Completed v Comments Basecode P N VER CHECKSUM Assay Config P N Duplicated Customer Complaint Comments UTIL TESTS and CHECKSUM If new software was Basecode P N loaded VER CHECKSUM Assay Config P N VER 52 if no download was performed Verification of Vertical and Horizontal Alignment PASS FAIL Comments Page 4 of 5 4070005 SD REV B SVCE TEST DATA SHEET Return Authorization 20 Shake Test PASS FAIL or 22 Comments Performance Testing PASS FAIL A
71. 375X 030 003 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070018 ACCESSORIES 384 UNIT B 1 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOLONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOLONLY 4070515 BOTTLE SUPPLY ASBY B 4 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070527 MANIFOLD 16 WELL ASBY B 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072073 PLATE MANIFOLD GLIDE C 1 4072077 STRIP GLIDE A 1 4072079 SPACER GLIDE STRIP B 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOLONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7101028 LABEL PATENT A 1 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H
72. 7 Aerosol Cover Assembly rev B As of 11 20 00 Item Description Rev Qty Remarks 12107 SCR PAN 6 32X1 2 PHILLIPS SS A 3 16003 LKWSHR 6 HELICAL SPR SS A 3 17055 WSHR FL 1561DX 3750DX 049 SS B 3 19396 SCR 10 32X 375 THUMB NYLON A 2 4070517 AS AEROSOL COVER ASBY B 0 DOCUMENT ONLY 4072020 COVER MIST B 1 4072021 COVER REAR MIST A 1 4072059 RETAINER BAR AEROSOL COVER A 1 48255 TAPE LAB 1 WIDE YELLOW A 0 A R 67316 CHANNEL RUBBER EXTRUSION A 0 08 98079 BAG POLY 12 X 18 B 0 BAG 1 98170 BAG ZIP LOC 3X4 A 0 BAG 2 5 26 Assembly Drawings 18 awa oos ps mes E O sacs ssnarw 3328 LON OG dHVHS Sag 46 919 9 NOILONGOYd O1 350313 gH SV 8 k GOLOV o NOUO3rotd 548 seon 9 21 9 0 10 OL 93 3 SLNSWNYLSNI 31 0I8 JO azis AN3SNO2 SS3HdX3 LOOHLIM 1 SV d E a N MAD G3SOTOSIC 38 TIVHS O3NIVINOO NOLIVWHOZNI HON 11 M3HLISN ON 31 018 MEENA 135 20 AVS Idod ONY T S00 POX SNININOO 1N3A OOG SIHL zu 9 3ov4uns X ZFX 910 8650 9080 DISOONINA 2669
73. 7 JIG HOLDER MANIFOLD A 0 TOOL ONLY 49903 FTG M LUER X 1 4 28 PP A 1 49906 FTG F LUER X 1 4 28 PP A 2 49908 O RING 1 4X1 32 70DURO EP A 1 98011 BAG ZIP LOCK SMALL A 0 99204 RUBBER BAND 7 X 1 8 X 1 16 A 1 4070513 Manifold Assembly 12 Well rev B As of 11 20 00 Item Description Rev Qty Remarks 12105 SCR PAN 8 32X1 2 PHILLIPS SS A 8 2872220 MP TEFLON DISPENSE TUBE ASBY MP D 0 DOCUMENT ONLY 2872538 JIG 0595 DIA TEFLON TUBE DRAW A 0 TOOL ONLY 4070513 AS MANIFOLD ASBY 12 WELL B 0 DOCUMENT ONLY 4072009 MANIFOLD WASHER 12 WELL G 1 4072011 TOP WASHER MANIFOLD E 1 4072012 GASKET WASHER MANIFOLD A 1 4072041 TUBE DISPENSE TEFLON B 12 4072041 DG TUBE DISPENSE TEFLON A 0 DOCUMENT ONLY 4072042 STYLUS TUBE ASPIRATE 0 TOOL ONLY 4072043 STYLUS TUBE DISPENSE C 0 TOOL ONLY 4072057 PACKING MANIFOLD A 1 4072507 JIG TEFLON TUBE CUTTING A 0 TOOL ONLY 4072508 JIG TEFLON SLEEVE INSTALL A 0 TOOL ONLY 4072517 JIG HOLDER MANIFOLD A 0 TOOL ONLY 49903 FTG M LUER X 1 4 28 PP A 1 49906 FTG F LUER X 1 4 28 PP A 2 49908 O RING 1 4X1 32 70DURO EP A 1 98011 BAG ZIP LOCK SMALL A 0 99204 RUBBER BAND 7 X 1 8 X 1 16 A 1 4070519 Manifold Assembly 8 Well Short Dispense rev A As of 11 20 00 Item Description Rev Qty Remarks 12105 SCR PAN 8 32X1 2 PHILLIPS SS A 8 4070513 AS MANIFOLD ASBY 12 WELL B 0 DOCUMENT ONLY 4072011 TOP WASHER MANIFOLD E 1 4072012 GASKET WASHER MANIFOLD A 1 4072046 MANIFOLD 8 WELL SHORT DISP E 1 4072047 TUBE DISPENSE TEFLON SHORT B 8 4072047
74. 8 HEDR 2 P 100 PCB MOUNT STR 1 121 42626 HEDR 10PIN 1 RT ANGLE A 1 J4 42627 HEDR 2P RT ANG LOCK POLAR A 4 112131620 46082 FUSE RESETABLE 5A PCB MNT A 1 49014 TERM PCB B 1 GND 49149 SOCKET IC 28 PIN DIP B 2 INU4041 49478 SOCKET 84 PIN PLCC A 1 INU48 49694 HEDR 10P 1 B 1 J 49743 CRYSTAL CLOCK OSC 32 MHZ B 1 2 62027 FILTER EMI SUPPR 5 1GHZ A 1 F1 63018 INDUCTOR 220UH 1 11 65020 BUZZER 3 16V PIEZO C 1 BP1 81004 CAP 100 UF 63V ELEC A 6 C2 694 81022 CAP 470 UF 35V ELEC VERTICAL C 1 c30 81027 CAP 10000UF ELECTROLYTIC A 2 C80 C12 16 17 21 22 26 3 83054 CAP 820PF NPO 100V 10 1206 B dor 83062 CAP 3300PF CER 1206 A 3 C14 9 24 84040 CAP 10 UF 25V TANT SIZE 7343 A 4 C45 53 57 58 C15 20 25 27 33 36 5 1 54 56 59 62 66 71 84041 CAP 1 UF 50V X7R SIZE 1206 A 36 75 78 80 83 85 86 88 90 92 95 97100 103 84066 CAP 1UF TANT 35V SIZE 3528 B 9 ME LE 85002 CAP 01 uF 100V 1 1 C108 Elx50 Strip Washer Service Manual 6 5
75. 8 HELICAL SPR SS A 4 16017 LKWSHR 10 HELICAL SPR SS A 2 17021 WSHR FL 20IDX 440D 1 16TK SS E 2 18034 WSHR SPRING WAVE 4 SS B 2 18038 WSHR 1301DX 2500DX 020T NYLON A 1 19112 SCR SOCK CAP 4 40X3 8 SS B 2 19140 SCR SOCK CAP 10 32X1 2 SS A 2 19143 SCR SOCK CAP 8 32X1 2 SS A 4 19197 RING RETAINING 1 8 SHAFT SS A 1 19198 SCR SET 8 32X3 16 CUPPT SS B 2 4070501 CABLE MOTOR LIFT ASBY B 1 CABLE E 4070509 AS LIFT MOTOR CAM ASBY C 0 DOCUMENT ONLY 4072006 CAM LIFTING D 1 4072007 ROLLER CAM A 1 4072014 BRACKET MOTOR MOUNTING C 1 4072015 MOUNT MOTOR B 1 4072504 JIG LIFTING CAM OFFSET B 0 TOOL ONLY 4072509 JIG RETAINING CLIP ASBY C 0 TOOL ONLY 45045 O RNG 3 1610 X 5 16OD VITON B 1 66289 LOCTITE 454 GEL 20GRAM A 0 A R 8050546 CABLE OPTO ASBY D 1 CABLE BM Elx50 Strip Washer Service Manual 5 17 133Hs L L avos a SV 0190720tr q D NOILOJ OYd STONY GHIHL A8SV WALSAS AlddNS 135 ONIGNL STULL ONI SLNAWNYISNI X31 018 40 1N3SNOO SS3HdX3 LNOHLIM Q3Sn a31voriana sio Q380 SI 38 G3NIVINOO NOLLVIWHOJNI 3 1 HON ONY SLNIWNYLSNI 31 018 30 ABV L3IBdOHd ONY IVILN3GIJNOO SNIVLNOO 1N3An2OQ SIHL s3903 A8 023 NOIlLdlMOS3Q 3105 LON oa 83d
76. 9 MANF 8 WELL SHORT DISP ASBY A 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 10 Bills of Material ELX50 12 Strip Washer 12 Well ELX50 12 Strip Washer 12 Well rev P As of 11 20 00 Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY T 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 05 FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLO
77. A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOLONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOLONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7101028 LABEL PATENT A 1 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 98011 BAG ZIP LOCK SMALL 0 98083 BAG 9X12 PLASTIC 2MIL 0 99125 LABEL 1 X1 2 CONTINOUS o o ARENT MODEL NUMBER Elx50 Strip Washer Service Manual 7 15 ELX50 16G Strip Washer 16 Well Generic ELX50 16G Strip Washer 16 Well Generic rev G As of 11 20 00 Item Description Rev Qty Remarks 16077 WSHR FLT 174X 375X 030 003 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070019 ACCESSORIES GENERIC 384 UNIT B 1 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOLONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOLONLY 4070527 MANIFOLD 16 WELL ASBY B 4 4070602 TEST STATION UQU
78. ADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOL ONLY 4070513 MANIFOLD ASBY 12 WELL B 4 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOLONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOLONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOLONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 99125 LABEL 1 X1 2 CONTINOUS MARK WITH MODEL NUMBER Elx50 Strip Washer Service Manual 7 11 ELX50 12G Strip Washer 12 Well Generic ELX50 12G Strip Washer 12 Well Generic rev P As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT
79. ANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 4 4072040 COVER TOP GENERIC D 1 4072073 PLATE MANIFOLD GLIDE C 1 4072077 STRIP GLIDE A 1 4072079 SPACER GLIDE STRIP B 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOLONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOLONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOLONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOLONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7101028 LABEL PATENT A 1 7332501 JIG OVRLY ALIGNMENT A 0 TOOLONLY 7710102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 98011 BAG ZIP LOCK SMALL 0 98083 BAG 9X12 PLASTIC 2MIL A 0 99125 LABEL 1 X1 2 CONTINOUS e ap MARK WITH MODEL NUMBER 7 16 Bills of Material ELX50 16V Strip Washer 16 Well w Valves ELX50 16V Strip Washer 16 Well w Valves rev G As of 11 20 00 Item Description Rev Qty Remarks 16077 WSHR FLT 174X
80. C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOL ONLY 4070512 MANIFOLD ASBY 8 WELL B 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER Elx50 Strip Washer Service Manual ELX50 8G Strip Washer 8 Well Generic ELX50 8G Strip Washer 8 Well Generic rev P As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT
81. Channel e Line 3 is less than 3 e Line 4 is less than 3 Residual Test Limits 8 12 Channel e Passes unless Residual Test Failure is displayed in the comments field 16 Channel e Average residual less than 1 00mg and must say PASS e Maximum residual less than 1 50mg and must say PASS Final Quality Check 24 Rinse washer with de ionized water 2X or until all dye is gone 25 Empty washer of fluid by running Prime with no fluid connected Do not run for longer than one minute without fluid 26 Clean the exterior of unit Verify all hardware is tight and all tubing is properly seated 27 Remove the manifold and place into the proper shipping container PAGE 5 OF 6 4070005 ST REV E SERVICE TEST PROCEDURE 28 Ensure all received accessories accompany the unit For Bio Tek Instruments demo units complete the demo checklist 29 Attach the data sheet to Job Sheet PAGE 6 OF 6 Return Authorization Bio Tek Instruments Inc MODEL ELX50 TITLE SERVICE TEST DATA SHEET PROCEDURE 4070005 SD SERIAL TECHNICIAN DATE STARTED Rev Description of changes ECO Date Release To Production 33883 10 03 00 B Correction in step 23 Accuracy Limits 35485 10 17 01 For Page 1 amp 2 use Certificate of Calibration Form SF8 NOTE Technician to complete form and mark Uncertainty of Measurement as 4 1 Reference Procedure 4070005 ST REV Other Required Documents 4070005 TP REV 40700
82. DG TUBE DISPENSE TEFLON SHORT A 0 DOCUMENT ONLY 4072057 PACKING MANIFOLD A 1 4072516 JIG SHORT TEFLON SLEEVE INSTL A 0 TOOL ONLY 49903 FTG M LUER X 1 4 28 PP A 1 49906 FTG F LUER X 1 4 28 PP A 2 49908 O RING 1 4X1 32 70DURO EP A 1 98011 BAG ZIP LOCK SMALL A 0 99204 RUBBER BAND 7 X 1 8 X 1 16 A 1 Elx50 Strip Washer Service Manual 5 21 vie 31VOS LON OQ 8 SV SLSOLOP sa A8SV AlddNsS l 311108 97186 OVE OILSW1d NI JYYdS JOWId 4020 ABSV 311108 Aldd Ns ESI 3i vdS S1N3S3sd3 SIHL c y geo TS y EN v vlad g 117130 CN NMOHS SV Al N ANU N 33S 09 Lg N v T zV 000L 29219 S p p ecoz40p n 9 NN SS wos L 01lv13a 33s 9 1VvLad 6 9 8 AHVSS3O3N OL TOHOOT ISN 6 NMOHS SV dVO NI 8 SNILLId HSNd AHVSS3O3N 4l NO ONIGNL IANS OL 10H09 1V HLIM SONILLIS JO SAVE 6086 OVE OLLSV Id NI ASSY 311108 313 4 00 3 310N OL SALON H 5 3 a 9 g V lv ASBY Page 1 of 1 4070515 AS Bottle Su 4070515 Bottle Supply Assembly rev B As of 11 20 00 Remarks DOCUMENT ONLY 10 Qty 0 0 83 Rev Description BOTTLE SUPPLY ASBY
83. E WITH MODEL NUMBER Elx50 Strip Washer Service Manual 7 13 ELX50 12GV Strip Washer 12 Well Generic w Valves ELX50 12GV Strip Washer 12 Well Generic w Valves rev R As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070513 MANIFOLD ASBY 12 WELL B 1 4070515 BOTTLE SUPPLY ASBY B 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE
84. EDR 25 MTAB TERM FMALE 25 PC MNT FILTER EMI BEAD 43 OHMS COMPRESSOR DIAPH 24VDC Item 4070401 AS 4070401 SC 4071401 42063 42664 62057 68062 Schematics 6 12 OS LOvOLOY L JO 133HS A8 003 NOILdlHOS3Q ZONIMvHG JWS 26 4 OWY NAMWVHG 16 2219 19262 NOI 2 OL 3SV3 13H V S VAONddV Aqsy 516113 92 81111 8660 70790 LA DISOONIM 866 Vd GNVIHSIH ONI SLNIWNYLSNI y3L Olg ddd UIEN 1 uSEAA wo 7 CX Se qeo jewod ay Woy pejepei Seusniq y Aq asiou sjue eJd Ajqwasse stu i o 3avas o voc o zavas o AAA EvVLeCvLOevLe 9304 spe g 911194 i OL er o qva o Ir 4070401 SC PCB Filters 6 13 Elx50 Strip Washer Service Manual 6 14 Schematics Section 7 Bills of Material This section contains the Bills of Material used in building the ELx50 Automated Microplate Strip Washer and its variations These documents are subject to change PART NUMBER DESCRIPTION ELX50 8 Strip Washer 8 Well page 7 3 ELX50 8G Strip Washer 8 Well Generic page 7 4 ELX50 8V Strip Washer 8 Wel
85. EE MODEL NUMBER Elx50 Strip Washer Service Manual 7 5 ELX50 8GV Strip Washer 8 Well Generic w Valves ELX50 8GV Strip Washer 8 Well Generic w Valves rev R As of 11 20 00 Item Description Rev Qty Remarks 4070002 ACCESSORIES GENERIC UNIT G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070232 SFTWR GENERIC ASSAY V1 04 A 0 TOOL ONLY 4070512 MANIFOLD ASBY 8 WELL B 1 4070515 BOTTLE SUPPLY ASBY B 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071003 OVRLY KEYBOARD GENERIC B 1 4072040 COVER TOP GENERIC D 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN C 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 77710
86. ELx50 Automated Strip Washer Service Manual ELx50 Automated Strip Washer Service Manual December 2000 COPYRIGHT 1999 PART NUMBER 4071008 REVISION E BIO TEK INSTRUMENTS INC ii Notices BIO TEK amp INSTRUMENTS INC Highland Park Box 998 Winooski Vermont 05404 0998 USA Phone 802 655 4040 888 451 5171 800 24 BIOTK Service BBS 802 655 4107 Technical Assistance Center Fax 802 655 7941 Sales 802 655 3399 Service E Mail sales biotek com Internet Site http www biotek com Coordination Center Europe Africa Bio Tek Instruments S a r l Parc d Activit s de l Observatoire 43 Avenue des Trois Peuples B t E 78180 Montigny Bretonneux France Phone 33 1 39 30 2276 Fax 33 1 39 30 0987 All rights reserved Copyright 1999 Printed in the United States of America Bio Tek Part Number 4071008 This publication is protected by copyright and all rights are reserved No part of this manual may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopying and recording for any purpose other than the purchaser s personal use without written permission of Bio Tek Instruments Inc Trademarks Bio Tek is a registered trademark and ELx50 is a trademark of Bio Tek Instruments Inc All other trademarks are the property of their respective holders iii Restrictions and Liabilities Information in this documen
87. HSIN DAENT LOW LAN cm Ser d X ZFX TOLYVINONY SLOFX RS N E LA 29659 suya HONS S3ONVH3101 NOLLONGONd i aiva FYNLVYNOIS wen SO z oO 900 20 OLNI HSNd 100240 I 4215 1002 0 NO 3 LJVHS YOLOW NI TIVLSNI 1V13 SHL NO XZ Sr0Sr MIYOS 13S NOV SM3HOS LAS 9 A8SV xc LSNIVOV LHOLL ONINIVLAN SIP N 86161 900240 NYO HSNd 60S240v 9c0gl LJVHS HOLOIN NO vOSZZO0r LH3SNI r Wy kal NAAOHS SV 1N3lHO _ SNITIVLSNI 9 zZ d3lS 335 ca T iz B G me 4315 vLOZLOF LZOLL N Ss 9 xc x wa a318vo sog 21091 e gt EM z 9 N er H3ONIJ lt xc OvL6L e 2 R 3 Qr 13S440 WvO NMOHS NS 0 p SNILJITOIP Xv vOSZZor 9109 e 9 NMOHS AINO Ss Xv IG 16 SY Wad ONLLNNOW OLdO CNV LAVHS HOLON 38 08L ASVA1 LV 13S OL SIF ISN 6 31VLOMH GINOHS Q3131dWOO N3HM YOLOW SNITIVLSNI N3HM G vOSZZOY e INO 38 LON GINOHS qe 3 318 9 LL SQv3sH 68299 3111001 A1ddV K SALON r H j E q 9 g 4070509 AS Lift Motor Cam ASBY Page 1 of 1 Assembly Drawings 5 16 4070509 Lift Motor Cam Assembly rev B As of 11 20 00 Item Description Rev Qty Remarks 16016 LKWSHR
88. L6 SIE 05886 NOILOnGO d OL 3SV313H v Sd8 L6 9 G tie6z 9101521 8 848 461919 49662 11086 OL SHO 9 Sd8 46 96 6267 40 OL ONIGNL dO HLON31 SHO a HSINIA S00500 t9 HSINI3 3ovdsns 8660 7090 LA ISOONIM 866 ONYTH IH SLNAWNYLSNI Q Q QO oX ZFX HVIDONV SLO X 1 MON S3ONVH310 NOLLONGOYd 261812 _ A8NNQ S3HONI NI SNOISNAWIG TIY 991519345 ASIMYSHLO 553 AHVSSON dl NO ONIEGNL ACIS OL IOHOO IV H LIM SONILLIS dO S84V8 34 8 c LL086 9V8 NI S3rT8WN3SSV Q3HSINIJ S L066v L066F 0S 00 vc 9049 SALON H 6 Sc SZ 6 9219 t N Sc 0t c9c249 089 N Sc 0t 2924 9 19089 ly System Assembly Page 1 of 1 Set Su 4070510 AS Tubin 4070510 Tubing Set Supply System Assembly rev D As of 11 20 00 gt Z IA zn z 22 gt c2 Py EO zm go eo N amp le w Salo lt lt lt a gt 2 a 105 x 2 em x 0 e am 0m 10110 Qe lt lt S mz D 6 Z
89. LS 3119995 3SIMHSHIO SS3TND 8v OLdO 8102 0 131 YEUEFOK 33s anavo 6v0Lv 9315 1V3d3s 38 das xz 1509 A371Nd dO WOLLOS OL 1139 08 4315 OS OG 9 SOIJAN 1 0 4 zs 1130 33S 0140 HONOL LON 5300 8 Q3H31N39 SI SVL O1dO JHL 8v09L was 1VH1 O1dO QHVMSO3 IV39 YOLOW ONISN LINN HOWE 810240 30118 08 318 xz xc teeth 40 1138 HSNd 88 d31S xz 3318v9 3 318v2 TIVLSNI 8 d31S 4009 LNO OV IS LNAWNOMY 3 318vVO ee2 8 2315 OL SYNS 38 31 dYYM N31HO9IL H3ONIJ HLIM 3 318v9 109 amna xc anavo 508 52161 KINO INO 3 19 LNNOW Wr auau NaON 3 318vO ge2 S dals 335 xz v gv OLdO 8105 09 erler xz xc 7081 NMOHS L ANO xz 91091 3903 1N3wNonv T NMOHS ATNO XZ xz LNOZIHOH N3LHOIL 150 IHL SI WvO IHL LZOLL G 30 3903 3H1 OS 9 3903 1 LSNIVOV 1715 SI WVO JHL OS 1331 A8SV WVO HOLOW 1311 HSNd 3 4315 viaa 8 x XZ SUVMCYVH 1N3WN I TV WVLNOZIHOH 85091 XZ TIVOIH3A N31H9LL SIF IHL NI xz 9315 SHL NO SNILLIS SI 3H1 OS ASSV WVO HOLOIN 1311 NOILOAS Y3ddN
90. N 0202407 Z OL v SALON 9065 r H 9 3 3 q 9 g V e Assembly Drawings 5 32 4070527 Manifold 16 Well Assembly rev B As of 11 20 00 Item Description Rev Qty Remarks 12101 SCR PAN 8 32X5 16 PHILLIPS SS A 5 19105 SCR SET 6 32 X 1 8 CUPPT SS A 2 19979 PIN DOWEL 1875 X 625 SS A 2 4070527 AS MANIFOLD 16 WELL ASBY B 0 DOCUMENT ONLY 4072042 STYLUS TUBE ASPIRATE C 0 TOOL ONLY 4072043 STYLUS TUBE DISPENSE C 0 TOOL ONLY 4072067 MANIFOLD DISPENSE 16 CH D 1 4072068 MANIFOLD ASPIRATE 16 CH C 1 4072069 MTG BLOCK MFD BRACKET B 1 4072070 PLUG MANIFOLD CHANNEL C 1 4072071 PIN GLIDE D 1 4072075 BRACKET DISP MANIFOLD MTG A 1 4072078 PACKING 16 CH MANIFOLD A 1 4072524 JIG 16 CH MANIFOLD ASBY B 0 TOOL ONLY 49484 O RING 203ODX 07DIA DUR70SIL C 1 49486 BUMPER ATS WASHER MANIFOLD A 3 49903 FTG M LUER X 1 4 28 PP A 1 49906 FTG F LUER X 1 4 28 PP A 2 49908 O RING 1 4X1 32 70DURO EP A 1 66294 LOCTITE ASSURE 425 A 0 99204 RUBBER BAND 7 X 1 8 X 1 16 A 2 Elx50 Strip Washer Service Manual 5 33 Assembly Drawings Section 6 Schematics This section contains the Component Layout drawing and Electrical Schematic for the ELx50 Strip Washer These documents are subject to change DRAWING DESCRIPTION 4070400 AS Main PCB page 6 3 4070400 SC Main PCB Page 1 of 6 page 6 6 4070401 AS PCB Filters Assemb
91. NT KIT F 1 SHIPPING 98079 BAG POLY 12 X 18 B 0 FOR MANIFOLD 98083 BAG 9X12 PLASTIC 2MIL A 0 AND CARRIER 98085 BAG POLY 26X32 2 MIL A 0 FOR UNIT 7 20 Bills of Material 4070018 Accessories Set 384 Unit 4070018 Accessories Set 384 Unit rev B As of 11 20 00 Item Description Rev Qty Remarks USED ON FINAL 12174 SCR KNURLED HD 4 40 X 5 8 SS A 3 FOR HOLDING MANIFOLD 2872304 PLUNGER DISP TUBE MANIFOLD XL D 1 4070510 TUBING SET SUPPLY SYSTEM ASBY D 1 4070511 TUBING SET WASTE SYSTEM ASBY A 1 4070515 BOTTLE SUPPLY ASBY B 2 4070517 AEROSOL COVER ASBY B 1 4071000 MANUAL OPERATORS ELX50 E 1 IN 2ND BOX 4071006 DECLARATION OF CONFORMITY B 1 IN MANUAL 4071007 PERFORMANCE VERIFICATION RPT A 1 4071015 INSTRUCTIONS CUSTOMER PACKING B 1 4072042 STYLUS TUBE ASPIRATE 1 IN TUBE 1 4073001 SHIPPING CNTNR ASBY W BOTTLES 0 44299 LABEL NO SHARP OBJECTS A 1 48140 BOTTLE 2L H D HDPE NALGENE C 1 48146 FILTER HYDROPHOPIC VACUUM LINE B 1 49744 SHELL 3 16 DIA X 4 5 8 OL A 1 TUBE 1 49745 PLUG 3 16 DIA CLEAR A 1 IN TUBE 1 61062 PWR SUPPLY 24VDC DESKTOP F 1 91083 BUBBLE BAG 8X11 5 A 0 INSTALLED BY 94075 SHIPPING DOCUMENT KIT F 1 SHIPPING 98079 BAG POLY 12 X 18 B 0 FOR MANIFOLD 98083 BAG 9X12 PLASTIC 2MIL A 0 AND CARRIER 98085 BAG POLY 26X32 2 MIL A 0 FOR UNIT Elx50 Strip Washer Service Manual 7 21 4070019 Accessories Set 384 Ge
92. NVW OLNO 00S240r Dir JO WALI 135 VOL d31S ANSWNOMW Q IO3NIVIW OF 18 sisoa oir IVNIA331 OLNO Lf 8 IWNIW33 L OLNO Zf 9rr1d are Ty 10020 GQ IO3INVNW SIf 0086105 40 WAL 13 0 v8 T 103INVW t 00 dais aas e ava1 0v18 a xz xe 9 e ONIAN 9NLLO3NNOO 310438 0091 diNnid Sdv9 SAOWSY gt 151 ONIMIM 335 LL dais 21 4315 33S 79089 OL ea 193NNO9 OL 401 39 fi H L dO ANI 183SNI 3 NMOHS SV 9 lt TIVM NYHL ONLLLIA 9 T1Nd wi OLNI SNILLIJ TIVISNI 40018 s ol StTOS 13VHS NO Q31V3S ATINA SI 9749 A8SV IANS 13419 NI LOTS OLNI 309 A8SV s uqayovug 30 WOLLOS 1804 L E Q0INVW 34OONVIS Sv LIVHS NO 3 A8SV 39v 1d ASSV Suv 1N3WLSnrOv 8 1N3W3NOIV anava G103INVW SIn e S066 009220 40 Z WALI L Ww 3 1 1 A8SV 3AIHQ 3ONIHAS er 0 0 318v2 6 vr LOFOZOV ava MOVIE a38vo er LOvOZOV avai 6318 9 NMOHS SV NVd 3SV8 NI Zr 000207 oLoN a 318 0 AASV 131 OIN otn cir O13O WS 313VO S09 AASV 141 Z066 90669 vir 0070207 3umvo ten NAHL vr A8SV SARC SONIAS IIS 82021 Sea wasu saw S310N NO NN
93. OLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 49900 PLUG 437DIA HOLE NYLON BLACK B 4 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 8 Bills of Material ELX50 8VS Strip Washer 8 Well w Valves Short Dispense ELX50 8VS Strip Washer 8 Well w Valves Short Dispense rev R As of 11 20 00 Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16 C 0 DOCUMENT ONLY 4070020 TP FINAL TEST PROCEDURE ELX50 16 D 0 DOCUMENT ONLY 4070230 SFTWR DOWNLOADED CODE V1 06 C 0 TOOL ONLY 4070231 SFTWR DWNLD ASSAY CONFIG V1 04 A 0 TOOL ONLY 4070515 BOTTLE SUPPLY ASBY B 1 4070519 MANF 8 WELL SHORT DISP ASBY A 1 4070522 KIT SUPPLY TUBE VLVE OPT ABY C 1 4070602 TEST STATION UQUANT QC ASSAY B 0 TOOL ONLY 4071001 OVRLY FRONT B 1 4071002 OVRLY KEYBOARD A 1 4072001 COVER TOP D 1 40725
94. ON 3 16ID X 3 80D A 1 33 8 2X 68062 COMPRESSOR DIAPH 24VDC B 1 7120595 IDLER PULLEY ASBY B 1 7330503 CABLE POWER INPUT ASBY F 1 733 CABLE B 7330506 CABLE OPTO 14L ASBY C 1 733 CABLE E 7332004 SPRING TORSION B 1 7332020 ARM BELT TENSION B 1 7332028 SPACER TENSION ARM C 1 7333002 CABLE ASBY RS232 INTFC 486CPU B 1 Elx50 Strip Washer Service Manual 5 13 48 548 awa 2699 NOltdluosaq OL V ise ianvos L 4 l s3903 syna mv O lt 31v9S LON OG V SV 80S0ZO0r s STONY CHIHI INI X31018 40 ON SNIMYSG azis 1N3SNOO SS3HdX3 LNOHLIM SH3H10 uo SY3H10 038079810 38 TIVHS NI3H3H1 3H1 A8SV avita 20 AHVI3IidObd ANY 00 FXXX BEC T MC UT area OL GL0 X 0 LA SON S3ONVH3 101 NOILONGOYd 36 S3HONI NI SNOISNAWIG TIY G3I3IO3dS ASIMYSHLO 5531 LGIELIZ NYO ct xal Oud gl dals 83903 3S3HL LSNIVOV LHOIL SIF HSNd LNAWNOITV 5 OIC FLS 0F N3GGIH NMOH
95. OO AgSV OL SO NNOO A8SV 1511 SNISIA A8SV r 9 4 3 q 9 g v Elx50 Strip Washer Service Manual 4070507 Mechanical Assembly rev K As of 11 20 00 Item Description Rev Qty Remarks 12028 SCR PAN 10 32X3 8 SS SLOT A 2 12077 SCR SOCK CAP 6 32X1 2 SST A 3 15011 NUT KEPS 8 32 B 2 15012 NUT KEPS 10 32 B 2 16003 LKWSHR 6 HELICAL SPR SS A 8 16016 LKWSHR 8 HELICAL SPR SS A 5 16017 LKWSHR 10 HELICAL SPR SS A 5 16048 WSHR CURVED 208ID 3220D SS A 8 16051 WSHR FL 1941DX 3120DX 04T SS B 4 16065 WSHR CENTERING A 1 17003 WSHR FL 1561DX 3120D B 1 17021 WSHR FL 20IDX 440D 1 16TK SS E 11 17033 WSHR FLAT 4 125X 250 SS B 2 17055 WSHR FL 1561DX 3750DX 049 SS B 4 17056 WSHR FL 219IDX 4380DX 049 SS 2 17059 WSHR FL 188IDX 3750DX 049 SS B 5 18033 WSHR 6 FENDER SS B 3 18034 WSHR SPRING WAVE 4 SS B 2 19055 SCR SOCK CAP 6 32X5 8 BLK A 1 19112 SCR SOCK CAP 4 40X3 8 SS B 2 19139 SCR SOCK CAP 10 32X5 8 SS A 4 19143 SCR SOCK CAP 8 32X1 2 SS A 5 19186 SEMS 6 32X1 4 ST PHIL ITOOTH A 7 19198 SCR SET 8 32X3 16 CUPPT SS B 4 19332 SCR SHLDR 6 32X 1870DX 375 SS A 4 19393 SCR 8 32X 375 ROLOK PHH THDFRM A 1 4070400 MAIN PCB ASBY E 1 4070401 PCB FILTERS ASBY A 1 4070500 CABLE MOTOR CARRIER ASBY B 1 CABLE A 407
96. OPHOPIC VACUUM LINE B 1 49744 SHELL 3 16 DIA X 4 5 8 OL A 1 TUBE 1 49745 PLUG 3 16 DIA CLEAR A 1 IN TUBE 1 61062 PWR SUPPLY 24VDC DESKTOP F 1 91083 BUBBLE BAG 8X11 5 A 0 INSTALLED BY 94075 SHIPPING DOCUMENT KIT F 1 SHIPPING 98079 BAG POLY 12 X 18 B 0 FOR MANIFOLD 98083 BAG 9X12 PLASTIC 2MIL A 0 AND CARRIER 98085 BAG POLY 26X32 2 MIL A 0 FOR UNIT Elx50 Strip Washer Service Manual 7 19 4070002 Accessories Set Generic Unit 4070002 Accessories Set Generic Unit rev G As of 11 20 00 Item Description Rev Qty Remarks USED ON FINAL 12174 SCR KNURLED HD 4 40 X 5 8 SS A 2 FOR HOLDING MANIFOLD 4070510 TUBING SET SUPPLY SYSTEM ASBY D 1 4070511 TUBING SET WASTE SYSTEM ASBY A 1 4070515 BOTTLE SUPPLY ASBY B 2 4070520 AEROSOL COVER ASBY GENERIC B 1 4071000 MANUAL OPERATORS ELX50 E 1 IN SECOND BOX 4071006 DECLARATION OF CONFORMITY B 1 IN MANUAL 4071007 PERFORMANCE VERIFICATION RPT A 1 4071015 INSTRUCTIONS CUSTOMER PACKING B 1 4072042 STYLUS TUBE ASPIRATE C 1 IN TUBE 1 4072043 STYLUS TUBE DISPENSE C 1 IN TUBE 1 4073001 SHIPPING CNTNR ASBY W BOTTLES C 0 44299 LABEL NO SHARP OBJECTS A 1 48140 BOTTLE 2L H D HDPE NALGENE C 1 48146 FILTER HYDROPHOPIC VACUUM LINE B 1 49744 SHELL 3 16 DIA X 4 5 8 OL A 1 TUBE 1 49745 PLUG 3 16 DIA CLEAR A 1 IN TUBE 1 61062 PWR SUPPLY 24VDC DESKTOP F 1 91083 BUBBLE BAG 8X11 5 A 0 INSTALLED BY 94075 SHIPPING DOCUME
97. RE ELx50 STRIP WASHER 20 21 22 23 24 25 26 Press the ENTER key to start the read Remove the printouts from the printer and observe the results For 8 and 12 channel manifolds under QC Results Accuracy pct err the third item on the list must be less than 2 5 CV Percent the fourth item on the list must be less than 2 99 NOTE Follow steps 12 22 are for 16 channel washers Use PC with Microsoft Windows 9X operating system and Microsoft Excel Setup Click on the Excel icon to open and File Open the test 384 16 channel accuracy xit Click on password READ ONLY Minimize excel by clicking on the on the upper right corner Open KcyJr by clicking on the KcJr icon and Select OK Open the protocol and select 384 630450 if not selected at the open protocol dialog Click on Open Now you are ready to read the plate NOTE Use the mouse to switch back to the KC jr program In the KC jr program select the 384 630450 protocol if not already selected NOTE When you read the plate ensure the uQuant Test Station 4070602 is at the main menu Click Read Plate and Read Plate at dialog Now click on OK The uQuant will read the plate and the absorbence data will be displayed This will take about 8 minutes to complete When the plate has finished and the data is showing click on the Transformation Tab these results are the solutions with the scratches from the plate
98. S NI SLOAN N NY 610 0 NALHOILL 39 28 6 NMOHS xc L686 910220v OL 6L02Z0v JO 3ovdsns LNOYS ONILNNOW xz 13 HO SNION38 900bL LNOHLIM 9If LSNIVOV SI 3ovdsns LNOYS OS 1SNIVOV 6106205 HShd 76 6 4102 0 SNIVSV JAIS Lv14 S NI SLNN NONY xz vOv f H d 3 q g 4070508 AS Carrier ASBY Paee 1 of 1 Assembly Drawings 5 14 4070508 Carrier Assembly rev A As of 11 20 00 Item Description Rev Qty Remarks 14006 NUT HEX 10 32 SS A 2 14041 NUT SQUARE 8 32 SS A 2 14042 NUT SQUARE 6 32 SS A 2 19394 SCR 10 32X 50 NYLON HEX HEAD A 2 4070508 AS CARRIER ASBY A 0 DOCUMENT ONLY 4072016 CARRIER MOLDED C 1 4072017 SUPPORT CARRIER B 2 4072019 SPRING MICROPLATE C 1 49187 FSTNR BLACK 6 32X1 2 B 2 49897 FSTNR BLACK 8 32X1 2 A 2 Elx50 Strip Washer Service Manual 5 15 aol isse L L anvos a fi s3903 ewing TV Ag awa 093 NoLLdldios3a zi 3 31vOS LON OG Sag 2699 ovtez NOILONGOUd OL 3993138 v 66 2 8 al 2 eee A8SV WYO E E N MOLO 4 WAHAN ONV ONI SINSHMSLSN LOIR
99. SEMBLY STAND 4072501 JIG LIFTING CAM ALIGNMENT 4072503 JIG MANIFOLD CALIBRATION 4072505 Elx50 Strip Washer Service Manual 8 1 Section 9 Procedures This section contains the Final Inspection and Test Procedures and Data Sheet for the ELx50 Strip Washer These documents are subject to change PART NUMBER 4070005 ST 4070005 SD 4070020 IP 4070020 TP 4070020 DS 8291007 AW DESCRIPTION Strip Washer Service Test Strip Washer Service Data Sheet Final Inspection Procedure ELx50 16 Final Test Procedure ELx50 16 Final Test Procedure ELx50 16 Data Sheet Field Software Download Procedure Elx50 Strip Washer Service Manual 9 1 9 2 Procedures CONFIDENTIAL Bio Tek Instruments Inc Model ELx50 8 ELx50 12 Strip Washers Procedure 4070005 ST Title Service Test Procedure Rev Description of changes ECO Date A Release to Production 2994 10 14 97 B Changed Step 10 to 13 and 13 to 10 30327 02 24 98 C Add Shake Test amp Custom Assay Check Off 30711 06 10 98 D Added 16 Channel Manifold Testing Calibrating 33883 10 03 00 E Correction to step 23 accuracy limits 35485 10 17 01 Equipment Required Requires listing on the SF8 document Equipment ELx50 Operator s Guide ELx50 Service Manual 24 Volt Power Supply Power Cord Set USA 2 ELx50 Supply Bottles BTI ELx50 Waste Bottles BTI 1 ELx50 Supply Tubing Sets BTI ELx50 Waste Tubing Sets BTI Strip Washer Final Inspection Procedure
100. SPR SS A 3 17055 WSHR FL 1561DX 3750DX 049 SS B 3 19396 SCR 10 32X 375 THUMB NYLON A 2 4070517 AS AEROSOL COVER ASBY B 0 DOCUMENT ONLY 4072020 COVER MIST B 1 4072039 COVER REAR MIST GENERIC B 1 4072059 RETAINER BAR AEROSOL COVER A 1 48255 TAPE LAB 1 WIDE YELLOW A 0 A R 67316 CHANNEL RUBBER EXTRUSION A 0 08 98079 BAG POLY 12 X 18 B 0 BAG 1 98170 BAG ZIP LOC 3X4 A 0 BAG 2 Elx50 Strip Washer Service Manual 7 23 Bills of Material Section 8 Recommended Service Replacement Parts The following are recommended service replacement parts for the ELx50 Strip Washer and its variations These are the most common parts that might have to be replaced during preventive maintenance or repair This list is subject to change Description Bio Tek Part Number Display LCD 2 X 24 Super twist 04018 Belt Timing 260T 1 4W D 41049 Overlay Keyboard 4071002 Main PCB Assembly 4070400 Tubing Supply Set Assembly 4070510 Tubing Waste Set Assembly 4070511 Bottle Supply Assembly 4070515 Syringe Pump Assembly 4070516 Operators Manual ELx50 Strip Washer 4071000 Cover Top 4072001 Shipping Container Assembly 4073000 Waste Bottle 48140 Top Case Mounting Screws 49748 Power Supply 61062 Valve Check 3 16 barbs PP V 2 1 68061 Compressor Diaphragm 24VDC 68062 Idler Pulley Assembly 7120595 Cable Opto Sensor 14L Assembly 7330506 Jigs for Assembly amp Calibration Bio Tek Part Number JIG MANIFOLD ALIGNMENT 4072500 JIG AS
101. STE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072521 JIG MICROPLATE STOP ALIGN 0 TOOL ONLY 4072522 JIG CARRIER POSITION CHECK A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 4072526 JIG AUTOCAL VERIFY B 0 TOOL ONLY 7101028 LABEL PATENT A 1 7332501 JIG OVRLY ALIGNMENT A 0 TOOL ONLY 7770102 LABEL FCC COMPLIANCE A 1 7771011 LABEL ETL WITHOUT UL B 1 7771012 TAG S N WITH CE MARK A 1 8290206 SFTWR DOWNLOAD UTIL V1 42 H 0 TOOL ONLY 9001018 LABEL IN VITRO DIAG USE A 1 98011 BAG ZIP LOCK SMALL A 1 x MARK WITH MODEL 99125 LABEL 1 X1 2 CONTINOUS C 1 NUMBER 7 18 Bills of Material 4070001 Accessories Set Bottles and Tubes 4070001 Accessories Set Bottles and Tubes rev G As of 11 20 00 Item Description Rev Qty Remarks USED ON FINAL 12174 SCR KNURLED HD 4 40 X 5 8 SS A 2 FOR HOLDING MANIFOLD 4070510 TUBING SET SUPPLY SYSTEM ASBY D 1 4070511 TUBING SET WASTE SYSTEM ASBY A 1 4070515 BOTTLE SUPPLY ASBY B 2 4070517 AEROSOL COVER ASBY B 1 4071000 MANUAL OPERATORS ELX50 E 1 IN 2ND BOX 4071006 DECLARATION OF CONFORMITY B 1 IN MANUAL 4071007 PERFORMANCE VERIFICATION RPT A 1 4071015 INSTRUCTIONS CUSTOMER PACKING B 1 4072042 STYLUS TUBE ASPIRATE C 1 IN TUBE 1 4072043 STYLUS TUBE DISPENSE C 1 IN TUBE 1 4073001 SHIPPING CNTNR ASBY W BOTTLES C 0 44299 LABEL NO SHARP OBJECTS A 1 48140 BOTTLE 2L H D HDPE NALGENE C 1 48146 FILTER HYDR
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103. The Assay Configuration disk part number will be called out on each instrument versions final assembly BOM The Assay Configuration Software installed on the load PC should be checked for the proper revision level each time the loading PC is used See set up appendix for instructions 2 0 Procedure 2 1 Connections Connect the Unit Under Test UUT in the following manner with the power switches OFF PAGE 4 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 2 2 Power Up Once all of the test equipment is connected turn on the PC Once the PC has completed its BOOT sequence switch on the ELx50 The ELx50 will display the following in sequence Power Up Sequence V Initializing then Power Up Sequence V Clearing Download Area then Power Up Sequence V READY FOR DOWNLOAD Note The top line of the display may vary with model but the display must say Ready for Download before proceeding If the instrument has something else on the display turn it off and on again and press the shift and 1 hidden key between the clear and enter keys at the same time This will erase the memory in the instrument Note If at any time the Main PCB is replaced tag the failed PCB with the appropriate version and rev of the firmware which is loaded as it is removed from the unit 3 0 Entering Windows If Windows program is present on screen go to para 4 Enter Windows 3 X 95 or
104. The Group ID will be either ELx800 ELx808 PowerWave u Quant ELx405 or ELx50 The names may vary as well if the Configuration files have been customized by Bio Tek Select the DOWNLOAD button The Configuration download will begin If the instrument and computer are not properly connected or if the Instrument display does not read Start Assay Download the following message window may appear Define Assay Download 4 Unable to start the download sequence Error Message Receive Error Check that the reader is ready for download and that the proper cable is being used 14 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE v Check that the cable is connected between the computer and the SERIAL port of the instrument It may be necessary to check change the configuration of the serial port in the Download Utility under Setup Serial Port If the instrument does NOT display Start Assay Download press the Shift and Hidden Key 2 simultaneously Hidden Key 2 is between the Main Menu and Previous Screen keys The instrument should display START ASSAY DOWNLOAD YES NO Reader START PROGRAM DOWNLOAD YES NO Washer Choose YES then double click on the DOWNLOAD button in the Download Utility start the process again After the configuration download is complete the instrument will re initialize and return to the Main Menu Check that the current software is loaded onto
105. W REV F FIELD SOFTWARE DOWNLOAD PROCEDURE Enter target subdirectory Please select the main subdirectory PATH where you wish Extensions Download Utility 800 files to be installed Drive C The Download Utility will be installed in subdirectory C XTENSION unless a different subdirectory name is entered Enter source drive Please select the diskette drive you will use to install assays The assay configuration files will be installed from a floppy diskette in drive a other floppy drive designators may be available at this screen It is recommended that the assay configuration files be loaded from a floppy drive PAGE 5 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE wINSTALL Message Ready to load diskettes Press to continue This may take a few minutes The Download Utility files are being loaded In most cases there is only one Download Utility disk When the installation of the Utility is complete an ICON similar to the icon below will be seen in Windows 3 1 or Windows 95 Remove the Download Utility diskette from the computer s floppy drive Extensions teles Download Utility 4 3 Insert the appropriate CONFIGURATION diskette into the floppy drive 4 4 Ifthe instrument display reads ASSAY DOWNLOAD Readers or READY FOR DOWNLOAD Washers double click on the Download Utility icon If the instrument is at the main
106. Y ERR 0400 Manifold motor failed positional verify MOTOR VERIFY ERR 401 Carrier motor failed positional verify PAGE 21 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Error continued Error Cause Code SYR VOLUME TO LARGE 0900 Syringe volume too large PROGRAM CHECKSUM ERR 0 00 Checksum failure assay number MANIFOLD AUTOCAL ERR OBOO Invalid manifold for calibration MOTOR VERIFY ERR Syringe motor failed positional verify UNDEF PROG TYPE ERR Undefined program type specified PROGRAM NUM ERR Invalid assay number PROGRAM SET ERR Invalid write attempt PROGRAM DEL ERR Invalid delete attempt INVALID BUFFER ERR 0COO Invalid buffer selected CAL CHECKSUM ERR ODOO Failed calibration checksum test DISPENSE RATE ERR Dispense rate is invalid for manifold type DISPENSE VOLUME ERR OFOO Dispense volume invalid for manifold type CNFG DATA ERR 1000 Necessary configuration data missing CNFG CHECKSUM ERR 1100 Failed configuration checksum test CAL DATA ERR 1200 Necessary calibration data missing MOTOR NOT HOMED ERR 1300 Manifold motor not homed successfully MOTOR NOT HOMED ERR Carrier motor not homed successfully MOTOR NOT HOMED ERR Syringe motor not homed successfully AUTOCAL ABORT ERR 1400 Autocal of washer was aborted MALLOC ERR 1900 Memory allocation failure ASPIRATE_POS_ERR 1A00 Carrier offset position is out of range for present manifold DISPENSE_POS_ERR
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108. asher software If Extensions Define Protocol BTI P N 8290501 is installed on your computer this Download Utility should not be installed Un install the Define Protocol before using the utility 3 When updated software is downloaded onto the Instrument any open assays that have been programmed on board the Instrument will be erased and will have to be re programmed Please print the assay definitions before WINWORD PAGE 1 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE downloading new software To do this attach a printer to the parallel port of the Instrument choose REPORT from the main menu on the Instrument then press ASSAY and choose the programmed assay definition to print This printout will detail all assay parameters except any formulas which may have been defined The formulas are printed as part of the assay results report printout refer to a hardcopy of an assay results report or step through the define formula section from the front keypad to obtain the formula definition Note The download process will set the reader s filter table to its original default configuration The user must also check that the reader s filters and filter table positions match after the download To check the reader s filter table press UTIL gt SETUP gt FILTER gt Scroll through each of the filters programmed positions and compare to the physical position on the filter wheel See reader s operation manual for instructions on ho
109. aterials that are not damaged by chemical decontamination with 0 596 sodium hypochlorite or 7096 ethanol There is a priming soaking trough that is the normal home position for the manifold and which is an integral part of the carrier Carrier to Manifold Positioning Carrier movements are controlled by a timing belt and microstepping stepper motor Positions are configured for 1x8 1x12 and 1x16 well columns Resolution of carrier position is 0 002 inches minimum Through the on board software the carrier position can be offset from the factory default settings to optimize washing performance in a specific plate type or with a specific chemistry Manifold Vertical Positioning The washing manifold moves vertically by a microstepping stepper motor control It is possible for the user to offset the vertical position of the aspirating tubes relative to the carrier surface to optimize washing performance This offset is in equal increments no greater than 0 005 inches The offset range is from 0 100 to 1 0000 inches above the carrier top surface The manifold vertical positioning design allows for the manifold to lift as the aspirate tubes touch the well bottom and in that way compensate for vertical misalignment of the manifold and strips Positive Displacement Pum Wash solution rinse solution and disinfecting solutions are delivered to the microwells by a built in positive displacement pump syringe pump The pump draws fluid from the supply b
110. ccuracy Test 8 12 Channel Plate Weight with Fluid 13 97 14 83 Line 3 of Printout 2 50 Line 4 of Printout 2 99 Accuracy Test 16 Channel Line 3 of Printout lt 3 0 Line 4 of Printout 3 0 Residual Test 8 12 Channel PASS FAIL Comments field on printout Residual Test 16 Channel Average residual 1 00mg and must say PASS Maximum Residual lt 1 50mg Says PASS I HEREBY CERTIFY THAT I HAVE REVIEWED ALL ACCEPTANCE DATA THAT THE PRODUCT MEETS SPECIFICATIONS AND IS ACCEPTABLE FOR RELEASE Service Technician Date Page 5 of 5 CONFIDENTIAL Bio Tek Instruments Inc Model Elx50 Strip Washer Procedure 4070020 IP Title Final Inspection Procedure ELx50 16 Rev Description of changes ECO Date A Release To Production 32594 07 21 99 Equipment and Materials Jig Autocal Verify 4072526 24 volt power supply 61062 Feeler Gauges 0 001 0 025 Detailed Procedure 1 0 Alignment Setup 1 1 Verify that the carrier has the same serial number as the instrument It is possible that an instrument will have one carrier for use with 8 and 16 channel manifolds and another for use with 12 channel manifolds 1 2 Verify that the carrier rests on both adjustable feet and on the rail without rocking Use the appropriate carrier for the manifold to be tested 1 3 Install Autocal Verify Jig 4072526 into the carrier The jig must be positioned correctly in the carrier One cor
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113. channel pipette w tips Power Supply 61062 Power Cord Jig Manifold Calibration 4072505 Jig Microplate Stop Align 4072521 Jig Autocal Check 4072522 Test Station pQuant 4070602 Jig Autocal Verify 4072526 Jig 16 CH Manifold Asby 4072524 Tool Stopper 3 Hole Waste Btl 4072518 Tool Inlet Distribution Line 4072519 needed for washers with valves only Supply Tube Valve Option Asby 4070522 needed for washers with valves only ELx50 16 Set up to Dispense 150 ul or 50 ul ELx50 16 Set up to Dispense 300 ul or 100 ul Thumb screws 4072066 PAGE 3 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 1 0 Introduction The ELx50 is a EB80C186 based computer which is assembled with no internal software installed other then two BOOT EPROMs The ELx50 display the following message at initial power up Power Up Sequence V READY FOR DOWNLOAD NOTE The first line may vary depending on the version and model number Represents any number At this point the ELx50 will do nothing else until it has had Operating Software and Assay Configuration Software downloaded to it Once operating software is downloaded the unit will need a configuration download which loads in assay definition parameters The need for two download operations comes about because we will in the future be manufacturing many different versions of this instrument Each version will have different assay definition parameters
114. companying documents Attention voir documents d accompanement Achtung siehe Begleitpapiere Atenci n vease los documentos incluidos Attenzione consultare la doc annessa P Caution risk of electric shock Attention risque de choc electrique Gefahrliche elektrische Spannung Atenci n riesgo de sacudida el ctrica Alta tensione in questo documento Alta tensione non significa tensione pericolosa come definito in IEC 417 gt Warnings Use the ELx50 Automated Strip Washer on a flat surface and away from excessive humidity When operated in a safe environment according to the instructions in this manual there are no known hazards associated with the ELx50 However the operator should be aware of certain situations which could result in serious injury Do not reach under the instrument during operation as the syringe pump may pinch your fingers Elx50 Strip Washer Service Manual 1 3 Hazards Warning Power Rating The ELx50 Strip Washer s power supply must be connected to a power receptacle that provides voltage and current within the specified rating for the system Use of an incompatible power receptacle may produce electrical shock and fire hazards Warning Electrical Grounding Never use a two prong plug adapter to connect primary power to the ELx50 power supply Use of a two prong adapter disconnects the utility ground creating a severe shock hazard Always connect the system power cord directly to a three prong
115. ct Yes The instrument will now wait for the download 3 The download is performed using the Download Utility Program part number 8290206 FW Start Windows 3 1 or Windows 95 on the computer Note If the Download Utility is already installed on your computer go to step 3 3 3 1 Install the Download Utility via the File Manager or Windows Explore Double click on the Download Utility s Winstall exe file 32 The install program will ask several questions it is highly recommended that the user accept all the default settings in the installation process Once the Winstall exe is begun you will see screens similar to those shown below PAGE 10 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE Enter target drive Please select the drive where you wish Extensions Download Utility 800 files to be installed on your machine Select C to install the Download Utility onto hard drive C Enter target subdirectory X Please select the main subdirectory PATH where you wish Extensions Download Utility 800 files to be installed Drive C EXTENSION The Download Utility will be installed in subdirectory C XTENSION unless a different subdirectory name is entered PAGE 11 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE Enter source drive Please select the diskette drive you will use to install assays The configuration files will be installed from a floppy
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117. e Duc Edd End QU ea 21 am den Oa e ea err ne er en mec A 23 Appendix D Glear ASSAVS u uuu ie E FOU EN ESEE 23 Appendix E Using KCjr Data Reduction Software 23 PAGE 2 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Equipment and Materials X X X Requires listing on the Lab Product Equipment Calibration Data form specified on the data sheet A PC 386 min with a 1 44 mb 3 5 floppy drive 2 COM ports mouse and 4 meg of ram min Windows 3 1 installed on the above PC A PC 486 min with a 1 44 mb 3 5 floppy drive 2 COM ports mouse and 16 megabytes of ram recommended Windows 95 microsoft Excel version 7 0 and KCjr installed on above PC 75053 Serial Cable 71072 Parallel Cable Any parallel printer with paper Firmware see BOM for what is required for the assembly being tested Costar flat bottomed 96 well plates BTI 98102 Costar 384 well plates 98174 2 ELx50 supply bottles BTI 4070515 1 ELx50 waste bottles BTI 48140 1 ELx50 supply tubing sets BTI 4070510 1 ELx50 waste tubing sets BTI 4070511 Precision Scale with a min resolution of 0 01 and capacity of 120 grams Tween 20 FD amp C 1 Blue dye Deionized water Gelman glass filter system for filtering dye 50ml beaker 2 Plastic reservoir 200 ul pipettes w tips 200 1000 ul pipettes w tips 50 200 ul 8
118. e Appendix D 6 0 Software Configuration Verification With the introduction of Assay definitions downloaded from a PC the need for determining if the assays loaded are the ones which we really want in the version of the ELx50 we are setting up If a foreign language was downloaded the display should now reflect the appropriate language From the UUT main menu press the Util soft key Then press the Tests soft key Then press the Chksum key A revision level and part number will appear on the display as will the code checksum Compare these against 407 XXXX SP MP version and checksum On the second display version and part number of assay config download will be displayed They should match 407XXXX SP version number Refer to BOM for proper SP MP 7 0 Autocalibration The Autocal procedure establishes an accurate manifold and carrier location for each washer All ELx50 Washers with 16 channel manifolds shall be calibrated in both 8 and 16 channel configurations All ELx50 Washers with only an 8 or 12 channel manifold shall be calibrated as 8 channel only 7 1 Setting Up and Running Autocal Note Install Serial Number Label and mark Serial Number on Carrier 1 Connect the ELx50 to the 24 volt power supply that will ship with the instrument and power on the instrument 2 Plug the connector for the 4072505 jig into the header which is underneath the left side of the washer The correct header to use is the one closest to the front
119. e broader Caution Sodium Hypochlorite Do not expose any part of the instrument to Sodium Hypochlorite solution bleach for more than 30 minutes Prolonged contact may damage the instrument surfaces Be certain to rinse and thoroughly wipe all surfaces Caution Wash Buffer Solution Although many precautions have been taken to ensure that the instrument is as corrosion proof as possible the washer is not sealed and liquids can seep into sensitive components Make sure that any spilled wash buffer solution is wiped off the washer Prolonged exposure to salt solution may corrode parts of the microplate carrier movement rail springs and other hardware Caution Warranty Failure to follow preventive maintenance protocols may void the ELx50 warranty 1 4 General Information Equipment Overview There are several models in the ELx50 Washer product line refers to a Generic instrument and V indicates a Valve instrument with the multiple valve hardware PART NUMBER DESCRIPTION ELX50 8 Strip Washer 8 Well Manifold ELX50 8G Strip Washer 8 Well Manifold Generic ELX50 8V Strip Washer 8 Well Manifold w Valves ELX50 8GV Strip Washer 8 Well Manifold Generic w Valves ELX50 8S Strip Washer 8 Well Manifold Short Dispense ELX50 8GS Strip Washer 8 Well Manifold Generic Short Dispense ELX50 8VS Strip Washer 8 Well Manifold W Valves Short Dispense ELX50 8GVS Strip Washer 8 Well Manifold Generic W Valves Short D
120. e tray then place it in the carrier Run disp then enter twice NOTE When testing 8 channel and 16 channel manifolds the First 6 columns of the plate are used When testing 12 Channel manifolds the first 4 rows of the plate are used 3 Place the plate in the carrier of the UUT with the 150 ul Evacuate Solution wells toward the back of the carrier Make sure that the orientation of the plate is in the carrier 4 From the Main Menu select RUN MORE ASPIR then press the OPTION key until residual qc test is displayed for 8 and 12 channel manifolds or the resid qc test384 is displayed for 16 channel manifolds and press the ENTER key to select this program 5 Use the number keys to Enter The Number Of Strips to process 6 or 4 Press the ENTER key again to save the selection 6 Press the ENTER key or the START key a final time to start the program The washer will aspirate the contents of the strips containing the Evacuate Solution When the program is complete return to the main menu and prime the unit to clean 7 Immediately remove the plate from the carrier and weigh it because the residual is small and it will begin to evaporate Record the weight 8 When testing 8 and 12 channel manifolds use an 8 channel hand pipette add 300 ul of DI Tween 20 to the wells containing the residual dye solution this will require dispensing more than once to each well When testing 16 channel manifolds use an 8 channel hand pipette and add
121. er test just prior to terminating the test and determine that it is aspirating and dispensing to the microplate wells and that there are no visible leaks NOTE It is important to maintain clean solutions and bottles and tubing for all testing of ELx50 washers Water and dye solutions which are recycled over several days will grow algae bacteria molds or other undesirable organisms Refer to Appendix A for maintenance recommendations for supplies 1 Add approximately 250 ml of DI H2O with 0 196 Tween to a clean empty waste bottle 2 Place the stopper of a Tool Stopper 3 Hole Waste Btl 4072518 firmly into the top of the waste bottle 3 Connect the 2 waste line fittings of the stopper to the manifold of the UUT and the vacuum line to the vacuum port of the UUT NOTE Do not over tighten the tapered luer fitting connections Finger tighten only If finger tight were rated 1 10 with 10 being hard this shall be a 1 4 Connect the short tube of a 4070510 Tubing Set to the inlet port on the washer manifold Connect the other end of this tube to the top port of the syringe of the UUT 5 Connect the long tube of a fluid inlet line 4070510 Tubing Set Supply System Asby to the fitting on the stopper and to the bottom port of the syringe of the UUT For ELx50s with valves connect the fluid inlet line from the output port on the back of the instrument to the syringe and use the Tool Inlet Distribution Line 4072519 to connect the su
122. ete it is important to set up the instrument for the appropriate manifold Press UTIL then SETUP then MANFLD and select the desired manifold IMPORTANT PAGE 8 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE To check your Instrument s NEW software configuration from the instrument s Main Menu press UTIL then TESTS then CHKSUM Record the information below and fax this portion to BTI Technical Assistance Center at 802 655 3399 so that we may update your instrument records If you are updating more than one instrument please make note of all serial numbers of updated instruments I have successfully updated my Bio Tek Autoreader Autowasher Model S N The new software installed is Version Code Checksum Version Company name Signature Date PAGE 9 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE B Loading Assay Configuration Files Only 1 Connect the instrument serial port to the computer port using the 75053 null modem cable Uf your computer has a 25 COM port it will be necessary to attach a 9 pin to 25 pin adapter to the computer end of the cable 2 Turn computer and instrument ON 2 1 the instrument press the Shift and Hidden Key 2 simultaneously Hidden key 2 is between the Main Menu and Previous Screen keys The instrument display should read START ASSAY DOWNLOAD YES NO Readers START PROGRAM DOWNLOAD YES NO Washers Sele
123. ew the Data Sheet For Completeness Ensure it has Been Signed off All Printouts are Attached 3 0 All labels are Applied Per BOM I HEREBY CERTIFY THAT I HAVE REVIEWED ALL ACCEPTANCE DATA THAT THE PRODUCT MEETS SPECIFICATIONS AND IS ACCEPTABLE FOR RELEASE Name or Inspector No Title Date PAGE 4 OF 4 CONFIDENTIAL Bio Tek Instruments Inc Model ELX800 ELx808 PowerWave uQuant ELx405 ELX50 and all variations of each Procedure 8291007 AW Title Field Software Download Procedure Rev Description of changes ECO Date A Release To Production 28381 11 19 96 B Updated DLOAD commands Add procedure for 29671 08 05 97 loading ASSAY CONFIG Add ELX50 and PowerWave information C Change PoweWave References 29972 10 21 97 D Add Filter Table note add reference to nQuant 31209 10 21 98 E Add Ref to ELx405 Remove Assay from Text 32306 04 06 99 F Add Ref to ELx50 16 Software 32679 08 10 99 Equipment Required A PC 386 min with a 1 44mb 3 5 floppy drive 1 COM port minimum mouse and 4 MB RAM Windows 3 1 or Windows 95 installed on the above PC 75053 Null Modem Serial Cable 8290206 FW Download Utility Instrument Basecode and Assay Configuration Software Diskettes see About This Document below Special Notes 1 We may refer to the EIx800 ELx808 PowerWave u Quant ELx405 or Elx50 as the Instrument throughout this procedure 2 The purpose of this Download Utility is to allow field upgrading of reader and w
124. g with water from the sink first Thoroughly wash all supplies in hot soapy water Rinse again with sink water Then rinse all with the alcohol SETTING UP FLUIDICTS TEST Prepare a solution of DI H20 with 0 1 Tween 20 by mixing 1 liter of Deionized water with 0 9 ml of Tween 20 Prepare the washer Evacuate Solution by mixing 1 liter of DI HAO Tween solution with 0 50 grams of FD amp C 1 blue dye This solution will be referred to as the Evacuate Solution Mix the solution well Prepare the washer precision and accuracy solution by mixing 1770 ml of DI H20 Tween with 30 0 ml of washer evacuate solution This is a 1 60 dilution This solution will be referred to as the 300 ul Solution Setup the 4070602 Test Station uQuant QC Assays Connect the printer to the parallel port of the test station Power on the test station and the printer Appendix B Download Computer Setup Adjustments to the default Windows and Extensions installation that needs to be done before the above procedure will function as described These adjustments will only need to be made once and will remain on the set up computers hard disk The mouse and keyboard will be used to manipulate files Click and Double Click refer to using the mouse to select something by moving the cursor to the item and depressing and releasing the left most mouse button PAGE 20 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER First Install 8290206 FW SFTWR DWNLD U
125. he UUT and the manifold will lower to a position just above the jig Verify that the manifold aspirate tubes do not touch the jig Verify that the manifold aspirate tubes are less than 023 inches above the surface of the Jig Verify that the difference between the aspirate tube which is closest to the jig surface and that which is furthest from the jig surface is less than 010 Inches Furthest Closest 010 Press the ENTER key The manifold will lift and the carrier will move further away from home and the manifold will lower into holes in the jig Verify that the manifold aspirate tubes have moved into the holes without catching the edge of the holes and lifting the manifold Press the ENTER key The manifold and carrier will return to home Remove the jig from the carrier and press the Main Menu key two times Mark the data sheet to indicate that the UUT has met all of the above criteria Repeat of sections 8 0 and 8 1 for a second or third manifold as necessary Refer to 4070005 IP Mechanical Alignment Check if UUT fails Autocal Verify 9 0 Fluidics Test See Page 20 For Solutions Mixtures Connect the Printer to the Parallel port of the Test Station Apply Power to the Test Station and the Printer 9 1 Accuracy Test PAGE 10 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER NOTE When testing 8 channel and 16 channel manifolds the first 6 columns of the plate are used When
126. he process for downloading both the Basecode and assay configuration software onto the instrument PAGE 2 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE To load the Basecode and configuration files follow Procedure A for Basecode and Configuration files To load just the Configuration files use the Procedure B for Configuration Download A Loading the Basecode and Configuration files 1 Connect the instrument serial port to the computer port using the 75053 null modem cable Uf your computer has a 25 pin COM port it will be necessary to attach a 9 pin to 25 pin adapter to the computer end of the cable 2 Turn the computer and instrument ON 3 BASECODE DOWNLOAD Insert the Basecode software diskette into the computer s floppy drive 3 1 Get to the DOS a gt prompt or appropriate floppy drive prompt 32 the instrument press the Shift and Hidden Key 1 simultaneously Hidden key 1 is between the CLEAR and ENTER keys The instrument display should read START CODE DOWNLOAD YES NO IMPORTANT To properly load the Basecode software the above message MUST BE displayed on the instrument If START ASSAY DOWNLOAD is displayed press NO and repeat step 3 2 making certain that Hidden Key 1 is pressed 3 3 Select YES The instrument display will read Clearing Download Area then Ready for Download 3 4 At the computer DOS prompt type DLOAD and press Return 4 The Instrument display wi
127. ick and hold the left mouse button on Well A1and drag the mouse down to well P1 and then across to well P24 This will highlight all 384 wells 15 Using the mouse click the button labeled APPLY The formula will be applied to all wells 16 Use the mouse to select the REPORTS tab 17 In the box labeled Report Banner type 384 Well Absorbance Data 630 450 18 Use the mouse to check the box labeled Formula Reports 19 Use the mouse to click the button labeled OK 20 At the main Screen select New Protocol You will be prompted to save the protocol 384 630450 select yes PAGE 24 OF 25 Certificate WO UNIT Bio Tek Instruments Inc MODEL 1 50 TITLE Final Test Data Sheet Elx50 PROCEDURE 4070020 DS SERIAL Rev Description of changes ECO Date Release To Production 32594 07 21 99 Add Ref To Metal Strip Verify Stylus Per BOM Modify Autocal Verification Section Delete Note Add Fluids Test Heading 32708 08 17 99 Rewrite To New Format 33806 08 16 00 For Page 1 amp 2 use Lab Product Equipment Calibration Data C of C Form QA70 02FORM7 NOTE Technician to complete form and mark Uncertainty of Measurement as 4 1 Reference Procedure 4070020 TP REV Note The Following Steps Correspond To The Order Of Steps On The Cal Procedure Attach All Printouts To The Back Of The Data Sheet Numbers In Paragraphs 8 0 9 2 Parenthesis Are The Steps In The Procedure ASSEMBLY ALIGNMENT Refer to 4070005
128. ide accurate and repeatable carrier and manifold positioning Hardware Controller The strip washer is a microprocessor driven device with memory sufficient to perform all functions described in the ELx50 Software Requirements Specification Bio Tek part number 4076200 and for storage of up to 75 washer programs Washer programs can be defined directly from the washer keypad or they can be downloaded to the washer from a separate computer via the RS232 port using the Define Reader protocol Manifold Aspirating from and dispensing into the microwells can occur through a 1x8 1x12 or a 1x16 manifold There is no automatic detection of which 1f any manifold is present Only one manifold is included with an instrument with the other manifolds available as accessories The manifolds are easily removed and serviceable by the user They are constructed in a manner which allows for and with materials that are not damaged by chemical decontamination with 0 5 sodium hypochlorite or 70 ethanol Elx50 Strip Washer Service The ELx50 16 models contain the patented dual manifold design The 16 channel manifold has 16 sets of dispense and aspirate tubes arranged in a fixed horizontal position relative to each other but located in two separate manifolds The two manifolds move independent of each other in the vertical axis This design overcomes the problem of fitting both dispense and aspirate tubes into the much smaller
129. ig _ Pass Fail 4 Verify Diff Between Closest And Furthest From Jig Is Less Than 010 _ Pass Fail 6 Verify Aspirate Tubes Have Moved And Do Not Catch On The Edge Pass Fail 10 Second or Third Manifold Completed Above Testing _ Pas Fal 20 8 12 Ch For Valve Units Also Record Line 3 Of Printout 2 5 2 5 20 8 12 Ch For Valve Units Also Record Line 4 Of Printout 0 2 99 29 16 Ch For Valve Units Also Record Accuracy Test Results 3 09 _ 29 16 Ch For Valve Units Also Record CV Error 3 09 _ 30 Multiple Manifold Units Manifold Passes Accuracy Tests Pass Fail 31 Valves A B C Ensure Proper Operation Of Each Valve With a Prime Pass Fail 13 8 12Ch Residual Test All Channels 0 0 4 00mg 25 16 Ch Residual Test All Channels Ave Residual lt 1 0mg Ave Max Residual lt 1 5mg Max Burnin Test 1 Hr Minimum Record Start and Stop Times Ensure Supplied Power Supply Can Be Properly Screwed Into Conn Threads Ok And The UUT Does Turn On Properly Indicating Power Supply Is Working 2 Shake Test Verify Carrier Shakes Burnin Testing Proper Cleanup Has Been Finished _ Completed 10 0 Labels And Accessories Applied Or With UUT Completed By Date QC NOTE A Qualified QC Technician Must Perform The Following Step Item Results Limits 0 Ensure UUT is Free From Scratches and Other Cosmetic Defects Revi
130. ing AutoPrime used to keep the manifold tubes moist and free from clogging e Support for the following languages English French German Italian and Spanish Note Contact Bio Tek for information on changing to a different language The 4070230 basecode supports all of the 4070201 features plus the following e Compatibility with the 16 channel manifold for 384 well plate washing e Ability to create Soak and Link programs 2 6 Functional Description The basecode is downloaded from a PC to the washer via the serial communication port e prepare the washer for basecode software download press SHIFT HIDDEN KEY 1 from the main menu Hidden key 1 is between the CLEAR and ENTER keys e To download the software exit to DOS and type A NDLOAD 1 and press Enter represents the drive containing the basecode diskette and 1 represents the com port gt For detailed basecode download instructions refer to 8291007 AW Field Software Download Procedure Configuration Software The ELx50 washer requires information downloaded from a configuration software disk containing default washer programs diagnostics programs and a configuration file config 50 It is NOT recommended that you modify the config 50 file The on board software provides ample parameters for adjusting horizontal positions dispense and aspirate height etc Contact Bio Tek engineering with any specific questions or
131. ispense ELX50 12 Strip Washer 12 Well Manifold ELX50 12G Strip Washer 12 Well Manifold Generic ELX50 12V Strip Washer 12 Well Manifold w Valves ELX50 12GV Strip Washer 12 Well Manifold Generic w Valves ELX50 16 Strip Washer 16 Well Manifold ELX50 16G Strip Washer 16 Well Manifold Generic ELX50 16V Strip Washer 16 Well Manifold w Valves ELX50 16GV Strip Washer 16 Well Manifold Generic w Valves Elx50 Strip Washer Service Manual 1 5 1 6 General Information Section 2 Functional Description Principles of Operation This section of the manual discusses the principles of operation for each sub assembly within the ELx50 Automated Strip Washer Overview The ELx50 Automated Strip Washer performs microwell strip washing functions consistent with Enzyme Immuno Assay EIA needs These functions include aspirating from and dispensing into 1x8 or 1x12 microwell strips and 96 and 384 well plates Flat round and V bottom strips are accommodated A variety of wash parameters are user programmable via the on board software This instrument is powered by an external 24 volt power supply An internal positive displacement pump delivers wash buffer to the microwells from a 2 liter supply bottle The positive displacement pump allows for control of a variety of flow rates and volumes An internal vacuum pump aspirates liquid from the microwells into a waste collection vacuum vessel Stepper motors prov
132. l claims under this Limited Warranty must be made in writing to Bio Tek Attention Service Department Purchaser must ship the Product to Bio Tek postage pre paid Bio Tek shall either repair or replace with new or like new at its option and without cost to the Purchaser any Product which in Bio Tek s sole judgment is defective by reason of defects in the materials or workmanship This Warranty is VOID if the Product has been damaged by accident or misuse or has been damaged by abuse or negligence in the operation or maintenance of the Product including without limitation unsafe operation operation by untrained personnel and failure to perform routine maintenance This Warranty is VOID if the Product has been repaired or altered by persons not authorized by Bio Tek or if the Product has had the serial number altered effaced or removed This Warranty is VOID if any of the Products has not been connected installed or adjusted strictly in accordance with written directions furnished by Bio Tek Batteries fuses light bulbs and other consumable items used in any of the Products are not covered by this Warranty Software utilized in conjunction with any of the Products 1s not covered by the terms of this Warranty but may be covered under a separate Bio Tek software warranty We will continue to stock parts for a maximum period of five 5 years after the manufacturer of any equipment has been discontinued Parts shall include all materials char
133. l Inspection Procedure 4070005 IP This is found in the ELx50 Service Manual Section 3 If this step fails perform the Auto calibration outlined in the Final Test Procedure 4070005 TP This test procedure can be found in the ELx50 service manual Section 3 0 Run a shake test per the procedure outlined in the 4070005 TP NOTE If the instrument has Basecode Part 4070202 downloaded Please go to the Performance Testing section Perform verification of the Vertical and Horizontal Alignment as described in Final Inspection Procedure 4070020 IP This is found in the ELx50 Service Manual Section 3 If this step fails perform the Auto calibration outlined in the Final Test Procedure 4070020 TP This test procedure can be found in the ELx50 service manual Section 3 0 Run a shake test per the procedure outlined in the 4070020 TP PAGE 4 OF 6 4070005 ST REV E SERVICE TEST PROCEDURE 23 Performance Testing For Basecode part 4070202 Perform the Residual Accuracy tests as described in the Final Test Procedure 4070005 TP Include copy of test results to the job sheet For Basecode part 4070230 Perform the Residual Accuracy tests as described in the Final Test Procedure 4070020 TP Include copy of test results to the job sheet Accuracy Test Limits 8 12 Channel e Plate Weight with Fluid 14 40 mg 3 13 97 14 83 e Line 3 of Printout is less than 2 5 e Line 4 of Printout is less than or equal to 2 99 16
134. l w Valves page 7 5 ELX50 8GV Strip Washer 8 Well Generic w Valves page 7 6 ELX50 8S Strip Washer 8 Well Short Dispense page 7 7 ELX50 8GS Strip Washer 8 Well Generic Short Dispense page 7 8 ELX50 8VS Strip Washer 8 Well w Valves Short Dispense page 7 9 ELX50 8GVS Strip Washer 8 Well Generic w Valves Short Dispense page 7 10 ELX50 12 Strip Washer 12 Well page 7 11 ELX50 12G Strip Washer 12 Well Generic page 7 12 ELX50 12V Strip Washer 12 Well w Valves page 7 13 ELX50 12GV Strip Washer 12 Well Generic w Valves page 7 14 ELX50 16 Strip Washer 16 Well page 7 15 ELX50 16G Strip Washer 16 Well Generic page 7 16 ELX50 16V Strip Washer 16 Well w Valves page 7 17 ELX50 16GV Strip Washer 16 Well Generic w Valves page 7 18 4070001 Accessories Set Bottles and Tubes page 7 19 4070002 Accessories Set Generic Unit page 7 20 4070018 Accessories Set 384 Unit page 7 21 4070019 Accessories Set 384 Generic Unit page 7 22 4070520 Aerosol Cover Assembly Generic page 7 23 Elx50 Strip Washer Service Manal 7 2 Bills of Material ELXS0 8 Strip Washer 8 Well ELX50 8 Strip Washer 8 Well rev O As of 11 20 00 Item Description Rev Qty Remarks 4070001 ACCESSORIES G 1 4070003 KIT GENERIC HDW FINAL ASBY J 1 4070005 AS FINAL ASBY STRIP WSHR 8 WELL N 0 DOCUMENT ONLY 4070020 DS FINAL TEST PROCEDURE ELX50 16
135. lS dais N SNIddIHS Hainoxoa 6016 H3AV Z HLIM dO dO1 AdVL L330v48 G1O43INVW SNIddIHS V 6 9215 33S SNIddlHS H3NNI XOS l340Vvug GQ IO3INVW HSNd 9 133 8 Q TOJINVW OL 32078 NI 3AOOH9 vlad 33S NOLLISOd JHL NI SI YATION 3HL OS NYNL SNIddIHS LHOddhs WalJr 018 SALON 9 8 a a g V Ry 2 2 4070005 AS Final ASBY Stri 5 7 Elx50 Strip Washer Service Manual 4070003 Kit Generic Hardware Final Assembl rev J As of 11 20 00 Item Description Rev Qty Remarks 15012 NUT KEPS 10 32 B 2 16016 LKWSHR 8 HELICAL SPR SS A 3 16017 LKWSHR 10 HELICAL SPR SS A 4 17021 WSHR FL 20IDX 440D 1 146TK SS E 4 17059 WSHR FL 188IDX 3750DX 049 SS B 3 19053 SCR SOCK CAP 8 32X5 16 BK A 3 19139 SCR SOCK CAP 10 32X5 8 SS A 2 19194 SCR SOCK CAP 10 32X3 8 SS B 4 4070507 MECHANICAL ASBY K 1 4072081 COVER BOTTOM BASE A 1 4072500 JIG MANIFOLD ALIGNMENT D 0 TOOL ONLY 4072501 JIG ASSEMBLY STAND A 0 TOOL ONLY 4072505 JIG MAINIFOLD CALIBRATION C 0 TOOL ONLY 4072513 JIG OVERLAY ALIGNMENT A 0 TOOL ONLY 4072518 TOOL STOPPER 3 HOLE WASTE BTL A 0 TOOL ONLY 4072519 TOOL INLET DISTRIBUTION LINE A 0 TOOL ONLY 4072523 JIG FOOT ALIGN A 0 TOOL ONLY 45056 FOOT RUBR 1 062ODX27 32H3 16ID A 2 49845 RUBBER FOOT 81 DIA GREY
136. ll read DOWNLOAD IN PROGRESS if using a COM port other than COMI Type DLOAD x x indicates the COM port number Examples COMI Type DLOAD COM2 Type DLOAD 2 3 5 The computer will display File size 3000513 may vary Bytes sent xxxxxx will be counting up 3 6 The download will take several minutes When complete the computer will display DOWNLOAD SUCCESSFUL The instrument will display either START ASSAY DOWNLOAD or will return to the Main Menu Remove the Basecode diskette from the floppy drive PAGE 3 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE 4 CONFIGURATION DOWNLOAD This download is performed using the Download Utility Program part number 8290206 FW Start Windows 3 1 or Windows 95 on the computer Note If the Download Utility is already installed on your computer go to step 4 3 4 Install the Download Utility via the File Manager or Windows Explore Double click on the Download Utility s Winstall exe file 4 2 The install program will ask several questions it is highly recommended that the user accept all the default settings in the installation process Once the Winstall exe is begun you will see screens similar to those shown below Enter target drive Please select the drive where you wish Extensions Download Utility 800 files to be installed on your machine Select C to install the Download Utility onto hard drive C PAGE 4 OF 16 8291007 A
137. ltaneously press the C key This is a copy command 20 Use the computer mouse and change to the EXCEL spreadsheet application Click on File selecting program test 384 16 channel residual xlt to change to the correct spreadsheet program 21 With the cursor in cell C3 press the CTR key and simultaneously press the V Key This is a paste command The data from the reader file will be copied into the spreadsheet program See 9 1 paragraph 8 22 Move the cursor to cell E23 and Enter The Weight including the decimal point Press the ENTER key when finished 23 Move the cursor to cell E25 and Enter The Serial Number of the UUT Press the ENTER key when finished 24 Move the cursor to cell E27 and Enter Your Name Or Initials Press the ENTER key when finished 25 Plug the printer into the parallel port of the computer with EXCEL installed Click on the print icon or go to file and select print 9 3 Burnin Testing Burnin testing may be run now or after completing the residual and precision and accuracy testing Each ELx50 shall be run for a minimum of 1 hour of burnin There is a burnin program loaded with the base code of each ELx50 This PAGE 17 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER program mimics a plate washing program and loops continuously until stopped The object of running the burnin program is to reveal any early life component failures which may occur The operator must observe the instrument und
138. ly page 6 12 4070401 SC PCB Filters page 6 13 Elx50 Strip Washer el Schematics 6 3 e awos 22 SHEN 3 LON OG gt E s 931831 D SV 00v040v 9 3H3M A3HL 1VH1 HOLVOIONI HLIM ANY Mau kemiaa 3328 iaooe TORUM 03 38 1VIH3S 38 OL 38V SSNEWASSV QHVOS L BOA NIN zinc ae sNVINOO INSNNOOOSIHL HSINIJ 39V4unS 89d OL LHOIL 38 LSNW 32VMS NOSNHOFW SENS ee YVINONV SLOTX mesmo an soon
139. make X in box go away Click on Clear Wash Programs 5 Now all assay software should be cleared Appendix E Using KCjr Data Reduction Software Creating a data reduction protocol 1 Use the mouse to double click the KCjr icon on the desk top or select START PROGRAMS Kcjunior and click the Kcjunior pop up box 2 Select OK and wait for the program to initialize 3 Select NEW PROTOCOL and enter a protocol name in the appropriate box Use the name 384 630450 This protocol will read a 384 well plate at 630 nm and 450 nm reference wavelengths PAGE 23 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 4 Use the mouse to select the READ METHOD tab 5 In the box labeled Read Method Type select MultiWavelength 6 In the boxes labeled Wavelengths nm select first 630 and then 450 7T In the box labeled Plate Geometry select 16x24 8 Use the mouse to select the TEMPLATE tab 9 Use the mouse to select Well Ids 10 At the box labeled Well Type Selections choose sample 11 Using the mouse click and hold the left mouse button on Well A1and drag the mouse down to well P1 and then across to well P24 This will assign Well Ids to all 384 wells Well A1 should read SMPO01 and well P24 should read SMP384 12 Use the mouse to select TRANSFORMATIONS 13 At the box labeled Transformation Formula use the mouse to click the down arrow and highlight the transformation formula WV 630 WV 450 14 Using the mouse cl
140. menu press the Shift and Hidden Key 2 simultaneously Hidden Key 2 is between the Main Menu and Previous Screen keys The instrument should display START ASSAY DOWNLOAD YES NO Readers START PROGRAM DOWNLOAD YES NO Washers Select YES on the instrument The instrument will display as stated in 4 4 Click on the Download Utility icon The floppy drive should become active while the PAGE 6 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE application is initializing The following screen or similar should appear on the computer Download Target Dialog Download Target C Reader ELx800 ELx808 uQuant Powerwave C ELx50 Strip Washer Select ELx800 808 Reader for ELx800 ELx808 uQuant and PowerWave Readers Select ELx50 Washer for the Elx50 Select ELx405 Washer for the ELx405 After Selecting click OK A screen similar to the one below should appear Download Utility J n 1 Version Language Download Language PAGE 7 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE The Group ID will be either ELx800 ELx808 PowerWave uQuant ELx405 or ELx50 depending on the Configuration software being installed The names may vary as well if the Configuration files have been customized by Bio Tek Select the DOWNLOAD button The Configuration download will begin If the instrument and computer are not properly connected or if the
141. n n ener ener 5 30 4070527 AS Manifold 16 Well Assembly Page 1 of 1 nennen enne 5 32 SEGTION Rlm 6 1 Schematics m 6 1 4070400 AS Main PGB ces ee 6 3 4070400 SC Main PCB Page Lof 6 upa aasan bete eerie edens 6 6 4070400 SC Main PCB Page 2 of 6 EEEE EEEE EE EE 6 7 4070400 SC Main PCB Page 3 SPO T u US aS S L DO a RPE Saa 6 8 4070400 SC Main PCB Page 4 of 6 rennen ener trennt retener trennen eren nennen 6 9 4070400 SC Main PCB Page 5 of 6 E Oa a enne nes 6 10 4070400 5 Main PCB Page 6 0f l e l tree tete tette eite 6 11 4070401 AS PCB Filters Assembly 6 12 4070401 SG POR Pillu eeiam ted in iter UR edits 6 13 DSEGTION ap 7 1 oil 01 Y ee 7 1 ELX50 8 Strip Washer 8 Well eee tese ete etie eret dede HR ER E Ee Re Eee dea 7 3 ELX50 8G Strip Washer 8 Well Generic nnne enne nennen nnne 7 4 ELX50 8V Strip Washer 8 Well w Valves esee eene enne nennen ener entente nennen nennen 7 5 ELX50 8GV Strip Washer 8 Well Generic w Valves sse 7 6 ELX50 88S Strip Washer 8 Well Short Dispense
142. ner of the jig is labeled 12 CH with an arrow pointing to the corner Another corner of the jig is labeled 8 or 16 CH with an arrow pointing to the corner Choose the appropriate corner for the manifold being tested and align that corner toward the front and against the right edge of the carrier 1 4 Place the 8 12 or 16 channel manifold on the manifold bracket Fasten the manifold in place with thumb screws 4072066 WINWORD PAGE 1 OF 2 4070020 IP REV A FINAL INSPECTION PROCEDURE ELx50 16 1 5 Configure the UUT for an 8 12 or 16 channel manifold whichever is appropriate From the Main Menu use the soft keys and select UTIL SETUP MANIFLD and press the soft key to select an 8 12 or 16 channel manifold Press the Main Menu key to return to the Main Menu 2 0 Vertical and Horizontal Alignment Check 2 1 2 2 to 2 3 2 4 2 5 2 6 2 7 2 8 2 9 2 10 2 11 Use the soft keys and select UTIL TESTS and press the shift key and the 1 hidden key simultaneously the 1 hidden key is located between the clear and enter keys Use the soft keys and press AUTOCAL VERIFY and then press the ENTER key The carrier will slowly move into the UUT and the manifold will lower a position just above the jig Verify that the manifold aspirate tubes do not touch the jig Verify that the manifold aspirate tubes are less that 023 inches above the surface of the jig Verify that the difference between the aspirate tube
143. neric Unit 4070019 Accessories Set 384 Generic Unit rev B As of 11 20 00 Item Description Rev Qty Remarks USED ON FINAL 12174 SCR KNURLED HD 4 40 X 5 8 SS A 3 FOR HOLDING MANIFOLD 2872304 PLUNGER DISP TUBE MANIFOLD XL D 1 4070510 TUBING SET SUPPLY SYSTEM ASBY D 1 4070511 TUBING SET WASTE SYSTEM ASBY A 1 4070515 BOTTLE SUPPLY ASBY B 2 4070520 AEROSOL COVER ASBY GENERIC B 1 4071000 MANUAL OPERATORS ELX50 E 1 IN SECOND BOX 4071006 DECLARATION OF CONFORMITY B 1 IN MANUAL 4071007 PERFORMANCE VERIFICATION RPT A 1 4071015 INSTRUCTIONS CUSTOMER PACKING B 1 4072042 STYLUS TUBE ASPIRATE 1 IN TUBE 1 4073001 SHIPPING CNTNR ASBY W BOTTLES 0 44299 LABEL NO SHARP OBJECTS A 1 48140 BOTTLE 2L H D HDPE NALGENE C 1 48146 FILTER HYDROPHOPIC VACUUM LINE B 1 49744 SHELL 3 16 DIA X 4 5 8 OL A 1 TUBE 1 49745 PLUG 3 16 DIA CLEAR A 1 IN TUBE 1 61062 PWR SUPPLY 24VDC DESKTOP F 1 91083 BUBBLE BAG 8X11 5 A 0 INSTALLED BY 94075 SHIPPING DOCUMENT KIT F 1 SHIPPING 98079 BAG POLY 12 X 18 B 0 FOR MANIFOLD 98083 BAG 9X12 PLASTIC 2MIL A 0 AND CARRIER 98085 BAG POLY 26X32 2 MIL A 0 FOR UNIT 7 22 Bills of Material 4070520 Aerosol Cover Assembly Generic 4070520 Aerosol Cover Assembly Generic rev B As of 11 20 00 Item Description Rev Qty Remarks 12107 SCR PAN 6 32X1 2 PHILLIPS SS A 3 16003 LKWSHR 6 HELICAL
144. of the instrument 3 Install part 1 the stepped plate portion of the jig into the carrier The tall steps are nearer the front of the instrument 4 Install part 2 for 8 12 ch the contact pin portion of the jig onto the manifold bracket of the washer being tested and hold in place with 12174 screws 5 Configure the UUT for an 8 or 12 channel manifold From the Main Menu use the soft keys and select UTIL SETUP MANIFLD and Press the soft key to select 8 or 12 Press the Main Menu key to return to the Main Menu PAGE 8 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Check off on the Data Sheet the Manifold s that are being tested 6 From the Main Menu use the soft keys and select UTIL TESTS and then press the shift key and the 1 hidden key simultaneously the 1 hidden key is located between the clear and enter keys 7 Use the soft keys press AUTOCAL MECH and YES The carrier will slowly move into the UUT and AUTOCAL will be performed 8 If the UUT is a 16 channel ELx50 then configure the UUT for a 16 channel manifold part 3 J From the Main Menu use the soft keys and select UTIL SETUP MANIFLD and Press the soft key to select a 16 channel manifold Press the Main Menu key to return to the Main Menu 9 On ELX50 16 remove 4072505 part 3 from the manifold bracket of the UUT and install 4072505 part 2 Hold in place with 12174 screws 10 Repeat steps 6 7 above 11 Remove the autocal jigs
145. or is open Carrier Axis Home Position The carrier home position is defined to be sensor home plus an offset This offset Carrier Home Offset is presently defined in System Configuration The only time that sensor home and the home position are the same is if this value is set to zero Manifold Axis Home Position The manifold home position is defined to be sensor home Syringe Axis Home Position The syringe home position is defined to be sensor home plus an offset This offset Syringe Home Offset is presently defined in System Configuration The only time that sensor home and the home position are the same is if this value is set to zero Verifying an Axis Manifold When the verification is performed on this axis the software compares the number of steps observed at its present position to the actual number of steps required to move the axis back to the sensor home position At the completion of any program the manifold position is verified There are two scenarios 1 Ifthe manifold is on the sensor or the observed motor position is less than 3 full steps from the sensor then the manifold is moved away from the sensor 6 full steps The manifold is then moved toward the sensor until the sensor is marked If the observed motor position is outside the range of 3 full steps to plus 3 full steps inclusive then an error is generated 2 Otherwise the manifold is moved toward the sensor until the sensor i
146. ottles and forces it through the dispense tubes of the manifold It is possible to dispense at one of nine different flow rates by running the Elx50 Strip Washer Service Manual 2 2 4 pump at different voltages The flow rate 1s selectable by the user within the on board software The on board software contains calibration values for each flow rate Vacuum Pump The ELx50 has an internal vacuum pump which is cycled on and off as needed Vessels and Connections There are two 2 liter supply bottles three 1f V version washer No self sealing pressure vessels are required for fluid supply Each supply vessel has a hole in its cap that retains a suction tube A single inlet supply tube to the strip washer is connected to one or the other of these supply bottles though a pair of fittings Typically one bottle will be used for wash buffer solution and the second bottle will be used for deionized water rinse The user will disconnect the supply tube from the suction tube of one supply bottle and connect it to the suction tube of the other supply bottle when switching from one solution to the other There is no supply bottle liquid level detection It is the responsibility of the user to monitor and refill the supply bottles The washer may or may not have the multiple valves hardware V version washers Washers equipped with this hardware prompt the user to enter a supply bottle designation A B or C at program run time and then u
147. ow to the right of the language field then select the appropriate language Then click on the box to the left of the phrase DOWNLOAD LANGUAGE Click on the Download button to initiate the download NOTE The group list box may not list Bio Tek ELx50 if the software version is different There will be only one group listed and its name should be close to the software version name in ISIS For exceptions see following note The UUT display will change to DOWNLOAD IN PROGRESS while the assays are being downloaded When the download is done the computer will display a download successful message and the UUT will be reset to the normal main menu screen This may take up to 15min to get to the main menu screen The UUT will usually ERROR IEO0 because the AUTOCAL sequence has not been run Press the Stop key to stop the beeper and return to the main menu Use the mouse to Click on OK FILE and then EXIT to return to the windows menu Perform software configuration verification per Section 6 0 and record on data sheet To download the custom assay disk 407020 as per BOM Follow instructions per Section 5 0 using 407020 as per BOM Perform software configuration verification per Section 6 0 and record on data sheet PAGE 7 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER If the define assay download is unsuccessful or you want to download new define assay files you must first erase the existing assay files Se
148. pply port of the bottle to the three inlet ports of the washer 6 If the washer is an ELx50 16 then install a 16 channel manifold on the washer and configure the washer for a 16 channel manifold 7 Start the burnin program From the Main Menu use the soft keys and press UTIL TESTS then press the shift and 1 hidden key simultaneously Use the soft keys and press MFG and BURNIN PAGE 18 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER NOTE If the wash manifold is catching on the edge of the Microplate wells 8 then the Autocal configuration is not correct and Autocal must be rerun When burnin cycling has completed press the STOP key to end cycling and disconnect the UUT from the fluid and vacuum lines 9 4 Verify Shaking 1 2 The burnin program includes shaking as part of its program During the shaking cycle observe that the carrier is moving back and forth and that the liquid is mixing in the wells Check Pass or Fail to indicate that shaking has been verified NOTE Repeat any failed test 9 5 Cleanup 1 Disconnect the DI 20 with 0 1 Tween solution from the UUT and connect supply bottle of plain Deionized Water Run the maintenance program DAY RINSE From the Main Menu select MAINT and press the OPTION key until DAY RINSE is displayed then press the ENTER key to select this program Repeat process For UUT with valves disconnect the fluid single supply line from the b
149. removed Highlight A1 P6 hold amp drag mouse of the data displayed in KC jr Press the CTL Key and simultaneously press the C key This is a copy command Minimize KCjr by clicking on the on the upper righter hand corner of the window Open excel by clicking on the excel tap on the taskbar this should have been minimized in step 12 ensuring the test 384 16 channel accuracy xlt is open The cursor must be at Excel cell C3 A1 well of the UUT Press the CTRL PAGE 13 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 27 28 29 30 31 32 Key and simultaneously press the V key This is a paste command The data from the reader file will be copied into the spreadsheet program Below the data Excel cell E23 enter Weight and cell 123 Valve or Non Valve information needed Plug the printer into the parallel port of the computer with EXCEL installed Print the results by clicking on the print icon Observe the results that were printed For 16 channel manifolds Accuracy and CV error lt 3 Both must state Pass For washers with multiple manifolds Accuracy and Residual tests must be performed on each manifold Repeat sections 9 1 and 9 2 for each manifold Remember to configure the washer for the manifold being tested For washers with valve option A B C Ensure proper operation of each Valve with a Prime Observe during the Prime the fluid is moving through the tube
150. ring up a screen which says Start Assay Download Y N In this case answer Yes and the Ready for Download screen will appear In any case the unit needs to display the Ready for Download screen before proceeding 5 0 Downloading Define Assay Configurations NOTE If unit was previously programmed you must clear the assays See Appendix D Clear Assays The following download loads actual assay definitions into the instrument under test Insert the Assay Config program disk 4070202 or 4070203 as per the BOM into the AX drive The display the UUT must say Ready For Download before proceeding If it does not Press the Main Menu key and then the shift and hidden key 2 simultaneously Press Yes for download PAGE 6 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Use the mouse to double click on the ELX50 Assay Config Download Icon from within the ELX50 Utilities dialog box The user then will be prompted to select whether the target is an ELx800 Reader or an ELx50 Strip Washer Insure that the ELx50 Strip Washer is selected The computer will read in the files from the disk in the A drive This will take some time A 50 series Download screen will appear on the computer screen Use the mouse to select File and then Open Click on Bio Tek ELx50 in the Group list box then click on OK This will bring in the assay definitions Ifa foreign language configuration is required select it now by clicking on the arr
151. s e zn 109 iNOS ca z 1asviud zi wwa 2 SOLON HANNY zu n s u RUA oa anoo anv ozo nee z ano so 4 oovon ano asay 1363 7 w Ha em w SONO SONO OzH s 19 ov IE ossewed a mA zo E v n zao za 92 L zo 90 zasvHd D zan zz sa 1 mm HA 7 eww r3 we in 102 or iNOS ea t zn iw a za a INWA LWA ioo T i 148 068 y YOLOW 1311 G 104INVW i 3 amn oa neo 5 99 sz N zr n T GND NA 100A anv L szo P n Schematics 4070400 SC Main PCB Page 5 of 6 6 10 28 00007 9 340 9 133HS awos s dod S IVAOBddV auu 660 090 LA DISOONIM 866 GNVTHOIH 3OV3331NI QVdA3M 4 um
152. s marked If the observed motor position is outside the range of 3 full steps to plus 3 full steps inclusive then an error is generated Syringe There are five distinct actions that are being tested to insure the syringe is functioning correctly 1 Atthe beginning of each syringe intake stroke the software checks to insure that the optical sensor is marked If the sensor is not marked an error is generated 2 During the syringe intake stroke the software detects how many steps the syringe travels before the sensor changes from marked to open If this number of steps falls outside the specified range syringe sensor clear Syringe FMEA Range then an error is generated 3 Atthe beginning of each syringe dispense stroke the software checks to insure that the optical sensor is open If the sensor is not open then an error is generated 4 After completion of a Dispense Prime or Wash Program the software checks to insure that the optical sensor is marked Ifthe sensor is not marked and error is generated Elx50 Strip Washer Service Manual Ba 5 When power up or self test is performed the number of steps from syringe home position to clear the sensor is determined syringe sensor clear If this value is greater than the value specified Syringe FMEA Offset then an error is generated This determined value is then used during step 2 along with Syringe FMEA Range to determine a failure Carrier
153. s marked accordingly A to A valve B to B C to C It is possible for the wiring to be incorrect causing it to select the wrong valve Initial The Printouts and make a second copy of each to be shipped with the instrument Attach original print out s to the data sheet 9 2 Residual Test Place a Costar flat bottomed plate on the balance and tare the balance Use a 96 well plate for testing 8 and 12 channel manifolds and a 384 well plate for testing 16 channel manifolds When testing 8 or 12 channel manifolds use an 8 channel hand pipette and add 150 ul of Evacuate Solution to one half of the microwell plate When testing 16 channel manifolds use an 8 channel pipette and add 50 ul of Evacuate Solution to 6 columns of the microwell plate Avoid getting dye on the rim of the wells This will cause unexplainable failures of the test The ELx50 Test Station with a 16 channel manifold may be used rather than the hand pipette method ELX50 150 ul TEST STATION SETUP 1 Select test manifold 8 12 or 16 channel 2 Depress the soft key utility setup manifold then select 8 12 or 16 and return to the main menu PAGE 14 OF 25 4070020 REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER Depress soft keys run then option key Select D150 programs for 8 or 12 channel manifolds or select Resid QC test 384 for 16 channel Hit enter main menu run prime option key to select New Buffer Prime then enter twice Weigh th
154. se that bottle when running the program There is one 2 liter waste vessel The waste vessel has a sealing closure with one inlet and one outlet It is connected to an internal vacuum pump and a vacuum is created in the waste vessel The vessel is strong enough to withstand the external atmospheric pressure present under these conditions The collection vessel also connects directly to the manifold and solution 1s aspirated from the test wells by the manifold and pulled into the collection vessel Functional Description An optional hydrophobic vacuum filter may be placed in line between the vacuum pump and the collection vessel This filter impedes aerosols from entering the vacuum pump and therefore the room and waste from being pulled into the vacuum pump in the event of a collection vessel overflow It is the responsibility of the user to monitor and periodically empty the waste vessel There is no waste vessel level detection Display and Keypad The washer is equipped with a 2x24 character LCD display and a 25 key keypad Power Supply The washer is supplied with a 24 volt external power supply and cable Decontamination All exposed surfaces are designed to be chemically disinfected with 0 5 sodium hypochlorite and 70 ethanol Aerosol Cover The fluid dispensing region is covered with an aerosol cover The aerosol cover is easily removed to provide access to the manifold and plate carrier Elx50 Strip Washer Service Manual DS
155. sher From the Main Menu select RUN DISP and press the OPTION key until accuracy qc test is displayed for 8 or 12 channel manifolds or the acc qc test384 is displayed for 16 channel manifolds Then press the ENTER key to select this program If this is a ELX50 model with valves then use the soft keys to select valve A Use the number keys to Enter the number of Strips to process 6 or 4 Press the ENTER key again to save the selection Press the ENTER key a final time to start the program The washer will dispense 300 ul or 100 ul to the wells depending on which manifold is in place When the program is complete press the MAIN MENU key Immediately remove the plate from the carrier and Weigh it Record the Weight Place the plate in the carrier of the nQuant reader test station with the strips containing the fluid to left side of the carrier or to the back of the carrier depending on which manifold is being tested NOTE Follow steps 17 20 for 8 and 12 channel washers Go to step 21 for 17 16 channel washers Use the soft keys to select READ and then press the OPTION key until the 1x8 ACCURACY or the 1x12 ACCURACY test is displayed Press the ENTER key to select this program Enter the Serial Number of the instrument being tested for the PLATE ID and press the ENTER key Enter the Weight including the decimal point and press the ENTER key PAGE 12 OF 25 4070020 REV D FINAL TEST PROCEDU
156. suggestions The configuration software is downloaded from a PC to the washer via the serial communication port using Bio Tek s Download Utility e To prepare the washer for configuration software download press SHIFT HIDDEN KEY 2 from the main menu Hidden key 2 is between the Main Menu and Previous Screen keys e For configuration download instructions refer to 8291007 AW Field Software Download Procedure Elx50 Strip Washer Service Manual 2 7 Functional Description Section 3 Error Codes Homing an Axis When any motor axis is moved to sensor home its position is set to 0 Sensor home and the home position are not necessarily the same The following is a description of how each axis is moved to sensor home For the Carrier and Syringe axis there are two ways the motor can move to sensor home 1 Ifthe carrier or syringe is on the sensor typically at home then the axis is moved off the sensor plus 3 full steps The axis 1s then moved toward the sensor until the sensor is marked 2 Otherwise the carrier or syringe axis is off the sensor and the axis is moved toward the sensor until the sensor is marked For the manifold the process is similar 1 Ifthe sensor is open typically at home then the axis is moved away from the sensor until the sensor 15 marked plus 3 full steps The manifold is then moved toward the sensor until the sensor is open 2 Otherwise the manifold axis is moved toward the sensor until the sens
157. t has been recreated during this step Please record this on the Data sheet 2 Record The following Information on the ELx50 Data Sheet 4070005 SD Return Authorization Serial Number Technician Date 3 Note the accessories received with unit and record on the receiving report document 4 Turn the instrument over and remove the Base Pan Inspect all PCBs and ensure all cables and components are seated and oriented properly For valve version units verify all tubing is snug on each fitting 5 Set DVM to measure DC Volts Attach to the positions noted below and measure the voltages Record the values on the data sheet PAGE2 OF 6 4070005 ST REV E SERVICE TEST PROCEDURE Connect a DVM positive lead to C8 and the negative lead to chassis ground Set Connect the DVM positive lead to C5 and the negative lead to chassis ground 6 Record EPROM Information that is labeled on the EPROMs Cleaning and Updates 2s Place the instrument on the Jig Stand Refer to the 4070507 AS drawing in the ELx50 Service Manual Remove the top shroud 8 Remove the Syringe Cap and inspect the inner Teflon compression ring for evidence of leaking Replace cap if leaking is evident Verify the plunger is clean and has a high gloss appearance 9 Remove the manifold cover and inspect the rubber gasket for wear and puncture holes caused by the cleaning stylus Note Do not lubricate the gasket 10 Clean the following
158. t is subject to change and does not represent a commitment by Bio Tek Changes made to the information in this document will be incorporated in new editions of the publication No responsibility is assumed by Bio Tek for the use or reliability of software or equipment that 1s not supplied by Bio Tek for its affiliated dealers Revision Revision Date A 8 97 B 4 98 C 6 98 D 8 99 E 12 00 Changes Released to Production Updated assembly drawings and Bills of Material Updated TAC phone numbers Edited reformatted text throughout for clarity Updated contact information for Coordination Center Europe Africa Added Section 1 General Information and Section 2 Functional Description Updated assembly drawings and bills of material to include support for the 16 channel manifold Updated service procedures BOM s and drawings iv Warranty This Warranty is limited and applies only to new products except for computer based software which is covered under a separate Warranty Policy manufactured by Bio Tek Instruments Inc Bio Tek Bio Tek makes no warranty whatsoever regarding the condition of used products Bio Tek warrants the instrument hereinafter collectively referred to as Products or Product for a period of one 1 year from the original purchase date against defective materials or workmanship This Warranty is limited to the original purchaser the Purchaser and cannot be assigned or transferred Al
159. testing 12 channel manifolds the first 4 rows of the plate are used SETUP 1 Set up the washer for the manifold to be tested From the Main Menu screen use the soft keys and press UTIL SETUP MANFLD and select the 8 12 or 16 channel manifold Install the appropriate manifold on the UUT Place the stopper of a 4070511 Tubing Set Waste System Assembly firmly into the top of an empty waste bottle Connect the two manifold outlet ports of the UUT to the waste line of this stopper Connect the vacuum supply line from the stopper to the vacuum port on the rear of the UUT Connect a buffer bottle containing 300 ul Solution to the washer inlet of the UUT using the long tube of a 4070510 Tubing Set Supply System Asby The washer inlet is the lower syringe port at the back of the instrument Make sure that there is a check valve in place and that the flow arrow is pointing in direction of the inlet port For instruments with valves connect the supply line from the lower syringe port to the outlet port on the back of the washer after shortening the line to 5 inches long Use a 4072519 Tool Inlet Distribution Line to connect valve A to the buffer bottle of 300 ul Solution Connect the manifold supply line to the manifold inlet and the syringe outlet port The manifold inlet port is the single port closest to the edge of the manifold The syringe outlet is the top syringe port at the back of the instrument
160. the instrument by pressing UTIL TEST CHKSUM The instrument display will scroll through the seven digit part numbers and versions of both the Basecode and configuration part numbers Check them against the list at the end of this procedure The software update is complete PAGE 15 OF 16 8291007 AW REV F FIELD SOFTWARE DOWNLOAD PROCEDURE IMPORTANT To check your instrument s NEW software configuration from the instrument s Main Menu press UTIL then TESTS then CHKSUM Record the information below and fax this portion to BTI Technical Assistance Center at 802 655 3399 so that we may update your instrument records If you are updating more than one instrument please make note all serial numbers of updated instruments I have successfully updated my Bio Tek Autoreader Autowasher Model S N The new software installed is Version Code Checksum Version Company name Signature Date PAGE 16 OF 16
161. tility Vx xx Reference BOM Follow the Procedure in 8291007 AW Second Modify Configuration and Add Functionality Change the Extensions Description to ELX50 Utilities Change the Description of the Define Protocol Download Icon to read ELx50 Assay definition download Create a shortcut in Windows with these Properities for Downloading Basecode Description ELx50 Code Download Command line A Dload Working Directory A V Appendix C Error Codes An error code is displayed on the Microplate washer as a four digit identifier The first character will be either O 1 or A O or a 1 indicates a non critical error and the instrument will respond to keypad input An A as the first character denotes a more serious error In this latter case turn off the washer Upon restarting the washer you should be able to enter commands into the keypad General Errors These errors indicate non fatal conditions that require attention If an error is displayed refer to the troubleshooting tips and checklists shown in Chapter 5 Contact Bio Tek s Technical Assistance Center for further instructions Error Error Cause Code ABORT ERR 0100 Wash function aborted NO_SENSOR_ERR 0200 Manifold motor couldn t find optical sensor NO_SENSOR_ERR 0201 Carrier motor couldn t find optical sensor NO_SENSOR_ERR 0202 Syringe motor couldn t find optical sensor AUTOCAL JIG ERROR 0300 Manifold motor couldn t find edge of jig MOTOR VERIF
162. ton Syringe Pump Polished 4072060 PROCEDURE 1 Verify the unit serial number is within the suspected serial number ranges If not then do not proceed 2 Remove the two flat head screws holding the syringe 3 Remove the syringe from the syringe piston 4 Inspect the Piston It should have a mirror finish If the finish is slightly dull and does not have a glossy mirror finish remove the syringe piston by removing the flat head screw that is located on the head of the piston 5 Install the new piston part 4072060 6 Re install the syringe 7 End of procedure 4 2 Bulletins Section 5 Assembly Drawings This section contains Assembly Drawings that are used in building the ELx50 Strip Washer These documents are subject to change DRAWING DESCRIPTION 4070004 AS Kit Slider Upgrade Page 1 of 1 page 5 3 4070005 AS Final ASBY Strip Wshr 8 Well Page 1 of 4 page 5 4 4070507 AS Mechanical ASBY Page 1 of 3 page 5 9 4070508 AS Carrier ASBY Page 1 of 1 page 5 14 4070509 AS Lift Motor Cam ASBY Page 1 of 1 page 5 16 4070510 AS Tubing Set Supply System Assembly Page 1 of 1 page 5 18 4070511 AS Tubing Set Waste System ASBY Page 1 of 1 page 5 19 4070513 AS Manifold ASBY 12 Well Page 1 of 1 page 5 20 4070515 AS Bottle Supply ASBY Page 1 of 1 page 5 22 4070516 AS Syringe Pump ASBY Page 1 of 1 page 5 23 4070517 AS Aerosol Cover ASBY Page 1 of 1 page 5 25 4070518 AS Seal and Cylinder ASBY Page
163. ts instructions diagrams and accessories that were furnished with the standard models THIS WARRANTY CONTAINS THE ENTIRE OBLIGATION OF BIO TEK INSTRUMENTS INC AND NO OTHER WARRANTIES EXPRESSED IMPLIED OR STATUTORY ARE GIVEN PURCHASER AGREES TO ASSUME ALL LIABILITY FOR ANY DAMAGES AND OR BODILY INJURY OR DEATH WHICH MAY RESULT FROM THE USE OR MISUSE OF ANY EQUIPMENT OR INSTRUMENT BY THE PURCHASER HIS EMPLOYEES AGENTS OR CUSTOMERS OTHER THAN THE EXPRESS WARRANTY CONTAINED HEREIN WE SHALL NOT BE RESPONSIBLE FOR ANY DIRECT OR CONSEQUENTIAL DAMAGES OF ANY KIND THIS WARRANTY SHALL NOT BE CHANGED OR MODIFIED IN ANY WAY WITHOUT THE EXPRESS WRITTEN PERMISSION OF AN OFFICER OF BIO TEK INSTRUMENTS INC Contents at a glance SEGTION m ep 1 1 M M 1 1 SECTION precem 2 1 F nctional Descriptions M 2 1 lasudle Bc EE 3 1 ER 3 1 SEG TOWN 4 1 Bulletins ooi sioe teo ue te uha 4 1 5 1 Assembly Drawing 5 1 SECTION Heer 6 1 SCHEMATICS 35 5 sccsssdsescocesonsssseucenss 6 1 ua 7 1 Bills Of Mat plal y A S cbsesvcvuecesssssvescvsnces
164. urn in bottle and connect and connect the fluid inlet lines 4072519 Run the maintenance program DAY RINSE again Repeat this step for valve A B C Disconnect all tubes from UUT and remove the carrier Take care not to mix the carriers from different washers since each carrier is aligned for a specific washer Pick the UUT off of the bench and tilt it back to drain any remaining fluid from the syringe pump If only one manifold is being tested and this is not an instrument with valves repeat step one NOTE Do not let the weight of the washer rest on the rear panel because this may damage the syringe cup which sticks out of the back of the instrument PAGE 19 OF 25 4070020 TP REV D FINAL TEST PROCEDURE ELx50 STRIP WASHER 7 Use a paper towel or acid brush with Isopropyl Alcohol to remove any blue dye stain from the fluid ports of the manifold and the washer 10 0 Label Application After successful completion of all tests apply regulatory labels per BOM and final assembly drawing This is to be done by a certified technician Not required for Service Appendix A Bottle and Supplies Maintenance Prepare batches of dye and Deionized water solution that are small enough to be consumed within two weeks Rinse the bottles before adding fresh dye Sanitize all supply bottles and tubing sets waste bottles and hoses once a month with 7096 isopropyl alcohol Dump residual dye and water down the drain And rinse everythin
165. vavceusse susceusssssonseuses snuvcsusnesueseskess osesosusevswucssedssseshsousie E ESE oN e SE 7 1 SECTION 8 1 Recommended Service Replacement Parts 8 1 SECTION 9 u 9 1 PFoC6 nesuy uluya 9 1 vi Table of Contents hnE s iii NETSA E E E E E EES iii Trademarks iii Restrictions and Erabilities uy SEN Aui a E a iv REVISION secte d ee eeu tete aoe ee iv ees SECTION 1 1 General INf OVATION M 1 1 Purpose and eee inn eee eiie de duce fees tete fie felice 1 1 1 1 Telephones ores MM AIME 1 1 MAN bos EIN UID UU T 1 1 Email itu ee aiti E 1 1 Abbreviations and Symbols eae tene OE E tee dre 1 2 Wag Sein 1 3 Hazards iis 1 4
166. w to access the reader s filter wheel 4 When updated software is downloaded into the Elx50 or ELx405 existing programs are retained The software download adds new programs to the existing programs on board the washer In the event the user attempts to download too many assays for the washer program to store an error message will be displayed during the download process About This Document The ELx800 ELx808 PowerWave uQuant ELx405 and ELx50 all models have two levels of software installed on board 1 The Basecode software and 2 The Configuration software All ELx800 instruments have the same Basecode software part number 7330202 FW All ELx808 instruments have the same Basecode software part number 7340201 FW All PowerWave instruments have the same Basecode software part number 7260201 FW All Quant instruments have the same Basecode software part number 7270201 FW All OEM ELx50 instruments have the same Basecode software part number 4070201 FW All other ELx50 instruments have the Basecode software part number 4070230 FW All Elx405 instruments have the same Basecode software part number 7100201 FW The configuration software part number is dependent on the model of the instrument Contact Bio Tek Instruments to obtain current software revisions and versions These two levels of software are loaded into the instrument s memory via a computer connected to the serial port of the instrument This procedure explains t

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