Home

Nihon Kohden Celltak MEK-6400

1. Check prime function Check draining function Check cleaning function Check dispensing function Check Item OK No Enter the value x R Acceptable R value WBC x10 UL lt 0 7 LY lt 5 2 MO lt 3 3 GR lt 11 4 Check X R values LY x10 uL MO x10 uL GR x10 uL MEK 3DN RBC x109 uL 0 29 lot no HGB g dL 1 0 HCT 96 MCV fL 3 6 MCH pg MCHC g dL PLT x107 uL 39 Closed Open Predilution Enter the value Meas data Calcoef Meas data Cal coef Meas data Cal coef WBC RBC Check current librati HGB ca ions HCT t coefficients MCV MCH MCHC PLT Closed Open Predilution Enter the value Meas data Calcoef Meas data Cal coef Meas data Cal coef eu ad WBC calibration RBC coefficients HGB HCT MCV MEK 3DN MCH lot no MCHC PLT Check paper feed operation WA 640VK a Check auto printing operation internal Check printing operation printer 7 Em 7 There is no dot missing on the printed paper External Check paper feed operation printer There is no dot missing on the printed paper Only necessary on the MEK 6400 analyzer 6 10 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Check Item OK No Check the bar code reader operation Check communication status when PC is connected Check that the 3 pin plug ty
2. 1 5 Data Storage Ss tec dias EET 1 5 Environmental Cohditions 2 tr it ined 15 to iz Tia Le OT LC Mc PP e A 15 Dimensions and Welght sitas E 1 6 Electromagnetic Compatibility ssassn een pese baee a 1 6 Iu C N Danae 1 6 Panel DESCriPtiON e n 1 7 AAA EE 1 7 Right Side Panel eR 1 8 i um a a a n 1 9 A ME CE 1 10 NEGA OO aes saci cia ter ease Sd Bede irons exces inet dm ep emt ti d SL Ee dca a 1 10 MEK 6410 sianie tein eiu aste eaaet dede edu nue ederet thse te iud a ede ce ende Eee 1 11 MEK 6420 MM Hee ER 1 12 Interference Substances cimil niii einn naire a dada 1 13 Service Manual MEK 6400 6410 6420 1 1 1 GENERAL 1 2 Introduction CAUTION To maintain the instrument in normal condition the user must perform the periodic maintenance Refer to Maintenance of the operator s manual This service manual provides useful information to qualified service personnel to understand troubleshoot service maintain and repair the MEK 6400 6410 6420 Automated Hematology Analyzer referred to as the instrument in this service manual The maintenance must be periodically performed because the instrument has fluid paths and precision parts Accordingly the user is responsible for performing the periodic maintenance The Maintenance section in this service manual desc
3. 6 3 DISPOSING Of Et E 6 4 Repair Parts Availability Policy 0 0 0 0 eee ine ent nnnm nnrrnnnn nennen ener nnns 6 4 Parts to be Replaced Periodically encisar en aa estate nace dana ap Rare ede Ese V E cene eR ne 6 4 Maintenance Schedule p 6 5 Displaying Operation History Screen oen eene tnter ma nexo nnb hee aXX n ERR o eR RR SR RAR RRA 6 6 Maintenance Check Sheet 5 c Der ne i Lea Fb as 6 8 Before Maintenance Procedure ssssssssssssseeeeneeneee nenne nennen nnnm enn RRE RR r rr rn naar nnne rnnt nnne 6 12 Strong Cleaning serseri ana eret tec a laa 6 12 Draining Measurement Baths and Sub Baths oococccnccccccnoccccnnoncnnonancncnnnncononn cnn adaa naaa eene enne 6 13 TMUMING POWER Off MeL EP 6 14 Daily Maintenance Procedures moco A A vibes cece a G 6 15 Checking Reagents and Other Consumables cooooccccnoccccnonccccnoncncncnnnnnnnannncanannn carac cnn rn nennen nennt nnns 6 15 Checking the Appearance of the Analyzer oooooocccinoccccnnnocccononccnonannnnnononanonnn nn nono oran nano cara nnn cn ran rra rnann ra nnns 6 15 Disinfecting the Surface of the Analyzer ooooconcccccinoccccnncoccnononccnonnn conca cnn cnn ono nano no nana rca nana na rannn nine 6 16 Cleaning the Surface of the Analyzer ooooconnoccconocccconooccconnnccnonnnconnnno cocoa n cnn nana cn rra n rn rr enne nnne 6 16 Check
4. Samples are not stirred enough Possible Cause Criteria Countermeasure The sample was not stirred thoroughly before measurement Stir the sample thoroughly before measurement Hematology analyzer failure Possible Cause Criteria Countermeasure Circuit malfunction Replace the MC 640V measuring unit with a new one RBC is outside 1 60 5 when circuit check is performed by pressing MENU key Replace the UT 7198 MEASURING board with a new one gt OTHER key gt CIRCUIT CHECK key Replace the UT 7192 AMP CONTROL board with a new one RBC sample error Replace the MD 640V combination syringe pump unit with a new one In closed mode replace the MS 641V cap pierce unit with a new one In open mode replace the MS 640V sampler unit with a new one Service Manual MEK 6400 6410 6420 2 13 2 TROUBLESHOOTING Poor PLT Reproducibility Background noise is high The following may be the main reasons for poor PLT reproducibility Background noise is high PLT measurement is highly sensitive Therefore the background noise must be within the acceptable range PLT lt 10 x 10 uL Before checking the PLT reproducibility check the background noise Samples are not stirred enough Measurement settings are inappropriate Dilution error Hematology analyzer failure Possible Cause Criteria Countermeasure The diluent is dirty Replace
5. H 4 30 Reattaching the POWER Board ssssssssssssseeeeseeeeennene nennen nnnnsennnnn seen tss enr rnn nennen nnns nennen nnn 4 31 Removing the MEASURING Board rim ceri ec eim ue dE a tne 4 32 Reattaching the MEASURING Board 2 ritiene tede npa aa sud g edd pn e bu Recon Sae eun dined 4 33 Removirigithe MIXED PUMP Board sucia a 4 34 Reattaching the MIXED PUMP Board ccoconnocccccccccononcccncnnnnnnnnnn cnn cnn nan rn cnn 4 34 Removing the PRINTER DRIVER Board ccccccoooccccccconononcnonononnnnncnccononnnnnnnononnnnnnnnnnnnnnnnnnnnncnnnnnnnnrnnnrnnnnnnnncncnnnnns 4 35 Reattaching the PRINTER DRIVER Board oooccccccccononcccnccnnonnnncnnconnnnnnncnnconnnnnnnn none nennen E 4 35 Removing the KEY BORA pe 4 36 Reattaching the KEY Board o0 eect eeeennr rere een eter reece eter eee nn nr nn rra ONNEN NARREN nennen ener nennen nnns 4 36 Removing the LIQUID SENSOR Board ret eii ei ein A ACT ARE eee 4 37 Reattaching the LIQUID SENSOR Board 55 ciat adaa eee pa ce Eun idad 4 37 Removing the ECD mc EET 4 38 Reattaching the ROB 4 39 Removing the Transformer sse nennen nennen RR R nn rr seen nhnnnnnr nnn nnne nnn 4 40 Reattaching the Transformer bte ten dresse E s a n 4 40 Service Manual MEK 6400 6410 6420 4 1 4 DISASSEMBLY AND ASSEMBLY Before You Begin Warnings and Cautions Required Tools 4 2 The procedures in this section tell how to remove replace and install m
6. 14 Remove the measurement baths by pulling them toward you Tube joint e bs yA Black O ring Black O ring 6 38 to remove them from the aperture and then pulling them downward If necessary remove filter joints on the RBC and WBC measurement bath assemblies by turning the tube connectors Steps 13 14 15 Place a cloth or tissue paper under your hand and remove the aperture cap by pulling it toward you If it is not easy to pull the aperture cap move it slowly left and right to remove it 16 Carefully rinse the aperture cap Remove all protein build up especially from the inside The condition of the aperture cap can be checked with a 100x microscope 17 If a clog or dust still remains in the aperture caps soak the aperture caps in CLEANAC 3 detergent for about an hour CAUTION Handle the aperture caps with care They can be damaged if a sharp object is used to clean them 18 Rinse the aperture caps with water and replace them in the original positions Make sure that the black O ring is facing the hole analyzer side NOTE When replacing the aperture cap do not push the aperture cap with your ball of finger Otherwise the aperture cap may be broken Service Manual MEK 6400 6410 6420 6 MAINTENANCE Shaft 19 Reattach the measurement baths so that the sub bath is in the measurement bath the shaft of the sub bath is in the tab of the
7. Motor driver on the UT 7193 POWER board failure Replace the board with a new one the sub bath spacer has rust or crystallization Sub bath metal plate spring which holds Remove rust or crystallization with a sandpaper Replace the sub bath spacer with a new one if the problem still occurs The sensor cannot detect the rotary movement of the sensor plate Sub bath sensor on the measuring unit failure Replace the sub bath sensor with a new one Cable s on the measuring unit is damaged Replace the cable with a new one 2 28 Service Manual MEK 6400 6410 6420 E101 BATH DRAIN ERROR The MP 640V pump unit does not drain the measurement baths 2 TROUBLESHOOTING Reason Possible Cause Criteria Countermeasure The pump rotor does not move The MP 640V pump unit is disconnected from the UT 7193 POWER board Reconnect or replace the cable with a new one Stepping motor failure Replace the MP 640V pump unit with a new one Motor driver on the UT 7193 POWER board failure Replace the board with a new one The sensor cannot detect the rotary movement Pump sensor on the pump unit failure Replace the pump sensor with a new one Cable s on the pump unit is damaged Replace the cable with a new one E122 CHECK SETTINGS E123 MEMORY ERROR Re enter the calibration coefficient and all other settings Then sample counting can be
8. eesen 2 25 A095 Power supply temp high oooooocccccnnonoccconnnonananccnnnnnnanncco nono nnnnaccnnnnnn 2 25 APPEARS ON THE RIGHT OF WBC MEASURED VALUE 2 26 APPEARS ON THE RIGHT OF MCHC MEASURED VALUE 2 26 C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or PE T Glumps app6eals mission Pru dite 2 27 OTHER FLA Sisi ii 2 27 Solving Problems from System Error Messages ococcccoccconoocccnonaconinnnnononancn conc ca nanannanna 2 28 E001 DILUTER INITIALIZE ERROR sse 2 28 E021 SAMPLER INITIALIZE ERROR seseeeeeeneennenns 2 28 E041 SUB BATH INITIALIZE ERROR coccccccocccononcnonononcnnnnnnaninnnnncnananancannrs 2 28 E101 BATH DRAIN ERROR seen nnns 2 29 E122 CHECK SETTINGS eiii tind bett aec se ce bises 2 29 E123 MEMORY ERROR iiit cnet e reti REE 2 29 E124 CIRCUIT ERROR seisine etansa To anar epo ne recess 2 29 E126 TOUCH PANEL ERROR coccccocccconncnconnnononnnnnnnnnnncnnonnnancannnnnnannncnnannss 2 29 E141 CAP PIERCE INITIALIZE ERROR esee 2 29 Service Maintenance Screens sessi 2 30 Displaying the SERVICE Screen esdire sinda ada aa nennen 2 30 CHECK MENU Screen sssssssssssssseseeeeeeeeeennee nennen enne rennen nennen 2 32 UNIT PD Sz e he Man ee ets 2 32 MID 64 OV COP A A 2 33 MSI MIELE 2 33 MS 641V MEK 6400 only iiinis iaiaaeaia 2 34 MV amp PUMP CHK ivi ancien ten rente en Shee nen e
9. HOY 04 05 16 21 LED On LED Off 1 5819 1 719 0 138 1 5804 Temp compensation 26 214 17 8 18 84 26 614 17 8 18 84 Diluent 4 434 Lysing reagent 4 614 With reagent lt 15 Without reagent gt 3 5 Y lt Temperature gt HGB unit 24 7 C MC unit 27 8 C Power board 24 9 C Service Manual MEK 6400 6410 6420 OK THPUT CAL HOY 04 05 16 21 SENSOR HOHITOR OS SENSOR SYSTEN ERR LOG Check these 4 values 5 7 5 ADJUSTMENT Cursor Correction factors WBC up WBC upper sensor WBC low WBC lower sensor RBCup RBC upper sensor RBC low RBC lower sensor 5 8 Check the values on the screen With diluent in the measurement baths all four values should be less than 1 5 V If not press the INPUT CAL key at lower part of the screen to change the correction factors 1 Touch the setting value or use the arrow keys to move the cursor to the setting value you want to change Move cursor Numeric keys 11 Enter the value using the numeric keys and press the Enter key When the value is increased the voltage also increases When the value is decreased the voltage also decreases iii Press the OK key to return to the SENSOR MONITOR screen and see if the 4 values are less than 1 5 V iv When the 4 values are less than 1 5 V go to the next step If not repeat steps i to 111 until a
10. In closed mode replace the MS 641V cap pierce unit with a new one In open mode replace the MS 640V sampler unit with a new one HGB sensor output failure Adjust the HGB variable resistance volume so that HGB ON item is 3 0 V HGB ON item is outside 1 5 to 4 5 V 1 V Refer to Adjusting the HGB Sensor Output Voltage in Section 5 range when sensor check is performed by Replace the MC 640V measuring unit with a new one pressing MENU key gt OTHER key gt Replace the UT 7201 HGB AMP board with a new one SENSOR MONITOR key Replace the UT 7202 HGB LED board with a new one Replace the UT 7192 AMP CONTROL board with a new one Poor RBC Reproducibility The following may be the main reasons for poor RBC reproducibility Measuring unit and fluid path are dirty Samples are not stirred enough Hematology analyzer failure Measurement bath rinse unit or fluid path is dirty Possible Cause Criteria Countermeasure RBC measurement bath 1s dirty Press the Clean key on the front panel to perform cleaning Perform strong cleaning Clean the RBC measurement bath Replace the RBC sub bath with a new one Replace the RBC measurement bath with a new one Drain path of the RBC measurement bath is Clean or replace the filter below RBC measurement bath with a new dirty one Rinse unit is dirty Clean the rinse unit RBC aperture is dirty Clean or replace the RBC aperture with a new one
11. AMP CONTROL Board sssssssssseeeseneeeeeneee nennen nennen entren nen nnn nent rnn nennen enn 7 2 eidcm N 7 4 MEASURING Board inscrita t us tune terested 7 5 HIGB AMP BOa rd RR 7 6 LIQUID SENSOR Board eene etn coria ee dai 7 6 MIXED PUMP BOALG a coccion dana 7 7 KEY BOA EM a care 7 7 PRINTER DRIVER BOATA rca ti 7 8 Section 8 Socket Pin Assignment ccce 8 1 ZK 820V Bar Code Reader SOCk8t ocococnoccconoccciconocccconcnccnnnnncnnonnncnnonn aca nnnnn cnn 8 2 serial Port 1 Serial POrt2 cuidan rai 8 2 Printer Socket ida e du nta cons ll ric ec 8 2 USB SOGKCE m EE 8 2 LIIS EUME E X 9 1 Connecting the Power Cord and Grounding the Analyzer ssssseeees 9 2 Connecting the Power Cord sssssssssssssseseeeeneneen nennen nennen enne enn 9 2 Equipotential Grounding siii lona 9 2 Connecting Tubes and Installing Reagent sessssseeeeeenennn e 9 3 Materials RO Quine ce mt 9 3 Connecting TUDES viii 9 4 Diluent TUDE Em 9 4 Brief LUE 9 5 Lysing Reagent TUDO crono es 9 6 Waste Fluid Tube ooooconnnncccncnononoccconcnnnnnnnccnnnnnnnnnncnn nennen nnne nnne nennen nnn 9 6 Turning Power OD Off 2 oec erre or rri rrt deber c 9 7 T rning On the POWOL critica 9 7 Cleaning the Fluid Path After Turning the Power On PRIME ON INSTALL 9 8 Turning Off the Power aiio iere ai
12. Service Manual MEK 6400 6410 6420 5 1 5 ADJUSTMENT General This section describes how to adjust the following sensors HGB sensor output voltage Upper and lower sensor output voltages of the manometer Liquid sensor output voltages To adjust the HGB sensor you need to remove the right side cover For the other sensors you only need to display the screen for adjustment You do not need to disassemble the instrument 5 2 Service Manual MEK 6400 6410 6420 5 ADJUSTMENT Adjusting the HGB Sensor Output Voltage The HGB sensor is located at the WBC measurement bath on the MC 640V measuring unit To maintain the measurement accuracy the HGB sensor voltage must be 3 0 0 1 V when the LED for HGB measurement is lit LED On and less than 0 5 V when the LED for HGB measurement is not lit LED Off When the HGB sensor output voltage is off the appropriate value the following alarm may occur for HGB measurement e 2 displayed beside HGB value HGB sensor voltage is below 1 5 V when LED On displayed beside HGB value HGB sensor voltage is above 4 5 V when LED On e displayed beside HGB value HGB sensor voltage is above 0 5 V when a eps FA NIHON KOHDEN CelltacK LED Off Y In the above cases the HGB measurement is not reliable The HGB sensor output voltage must be adjusted 1 Turn off the instrument by pressing the Power key while holding down the Reset key The pow
13. 11 HGB unit any value is OK as long as no alarm is displayed 12 MC unit any value is OK as long as no alarm is displayed 13 Power board any value is OK as long as no alarm is displayed 4 Turnon the instrument and fill the manometers with reagent 5 Display the SENSOR MONITOR screen and check the following values With reagent in the manometers 14 WBC manometer upper 1 5V 15 WBC manometer lower 1 5V 16 RBC manometer upper 1 5 V 17 RBC manometer lower lt 1 5 V 18 Diluent 1 5V 19 Lysing reagent I 5V If a check result is outside the normal range sensors must be adjusted Refer to Section 5 Adjustment to adjust the sensors 6 Press the OK key to return to the OTHER screen Checking the Circuit Check the analyzer s electrical circuit 1 Press the OTHER key on the MENU screen GD EJ Aa Quiz 2 Press the CIRCUIT CHECK key on the OTHER screen HOY 04 05 16 40 MA VIA Gu MAINTENANCE GD GEL BACKGROUND Service Manual MEK 6400 6410 6420 6 41 6 MAINTENANCE 6 42 3 The analyzer starts checking the circuit and the screen shows the Checking circuit message When the check is completed the result appears HOY 04 05 15 15 WBC 0 0 RBC 0 00 HGB HCT MCV 0 0 MCH MCHC PET 0 18 a HGB ON 2 014 HGB OFF 0 12V Check that the results are in the following ranges Normal range WBC 7 6 to 8 4 10 uL RBC 1 52 to 1 68
14. A001 No diluent Press RESET key after replacement RESET ALARM HOY 10 05 14 20 Reagent check N Operation check EN C A005 No detergent Press RESET key after replacement Background noise check ERN C a A007 No lysing reagent Maintenance check ENN GoD Press RESET key after replacement RESET ea Service Manual MEK 6400 6410 6420 RESET ALL ea 9 7 9 INSTALLATION Cleaning the Fluid Path After Turning the Power On PRIME ON INSTALL The fluid path inside the analyzer must be cleaned after installation or long term storage Perform PRIME ON INSTALL on the OPERATIONS screen For details refer to Using the Analyzer after Storage in Section 9 of the Operator s Manual HOY 04 05 16 21 1 Press the MENU key on the READY screen QC CALIBRATION SETTINGS DATA OPERATIONS 2 Press the OPERATIONS key on the MENU screen OTHER 3 Press the PRIME ON INSTALL key on the OPERATIONS screen A confirmation message appears on the screen SAI HOY 09 05 18 50 OPERATIONS HOY 09 05 18 53 d PRIME DRAIN BATHS Initial prime after PRIME ON INSTALL DRAIN ALL installation CD UU F YES NO 4 Press the YES key to prime the analyzer Press the NO key to cancel the procedure and the screen returns to the OPERATIONS screen After priming the READY screen appears 9 8 Service Manual MEK 64
15. Adjustment ad edo HOY 04 05 16 21 lt Electrode gt LED On LED Off HGB 1 5819 1 719 0 138 1 580V Temp compensation WBC 26 21V 17 8 18 8V RBC 26 61V 17 8 18 81 lt Mano Reagent gt Upper Lower WBC manometer 3 924 4 334 RBC manometer 4 164 Z 4 259 Diluent 4 434 Lysing reagent 4 614 With reagent lt 15 V Without reagent gt 3 5 Y lt Temperature gt HGB unit 24 7 C MC unit 27 9 C Power board 24 9 C POS SENSOR screen No function 2 38 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING ALARM LOG aay HOY 04 05 16 21 Date Time Seqt Alarm ALARM LOG screen Displays all the alarm data with date and time if they occur You can get the problem tendency using this log data For example if some manometer dirty message occurs frequently you can try to perform the strong cleaning Total data 0 Page 41 1 e SYSTEM ERR LOG screen Displays all the system error data with date and time so you can see any HOY 04 05 16 21 Date Time Seqt System error problem trends Total data 0 Page 1 1 e au X10 CV screen HOY 04 05 16 21 This screen allows you to check the data reproducibility of the latest 10 Ho 21 30 10 31 40 10 MEAN measurement data X CV X CV X CV AC M AY At 3 A sa After the instrument is repaired or the measurement results are unstable use this HGB 1
16. Countermeasure Manometer is dirty The RBC manometer is dirty Perform strong cleaning Sensor voltage adjustment error error RBC manometer lower sensor adjustment Adjust the RBC manometer lower sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in Section 5 Service Manual MEK 6400 6410 6420 2 23 2 TROUBLESHOOTING A061 HGB voltage low This message appears when the HGB BLANK diluent voltage is less than 1 5 V Reason Possible Cause Criteria Countermeasure Deterioration from WBC measurement bath is dirty Clean or replace the WBC measurement bath with a from the LED not receive the light photodiode age new one HGB voltage adjustment error Adjust the HGB voltage Refer to Adjusting the HGB Sensor Output Voltage in Section 5 Erroneous HGB measurement started when there was After cleaning perform priming and measure again operation blood sample in the WBC measurement bath or the hematology analyzer was drained Photodiode does There are crystals or other dust on the Remove the cause Circuit error The UT 7201 HGB AMP board failure Replace the board with a new one The UT 7202 HGB LED board failure Replace the board with a new one The UT 7198 MEASURING board failure Replace the board with a new one The MC 640V measuring unit failure Replace the unit with
17. Dimensions and Wagi esson a xo aba s 1 6 Electromagnetic Compatibility esseseeeeneenns 1 6 EC P M M Ea 1 6 Panel Description M EE 1 7 Eront sine 1 7 Right Side Panel icon iia 1 8 Rear Panel sco xen tox ent teen Mute COTES EEERRM ERAT Ee EE REMRAEENE RE anai 1 9 Seien PER 1 10 zonis EE 1 10 MEK GATO ss freee cpa rcH EE 1 11 MEK 6420 iii allie iA be iia es 1 12 Interference SUDSIANCES e aa Eaa aaa naa 1 13 Section 2 Troubleshooting imc ani n 2 1 Check Procedure Flowchart ssiri iaeoe aaa ataia iaaea a ae E Eaa 2 4 Operating Condition and Blood Sample Checks oooococonoccccncccccconaccconannnanonancnnannncnnannnnanna 2 5 Operating Condition Check ssssssssseseeeeeeneen nennen nennen nnne 2 5 Blood Sample Handling CHECK sistis mnra a 2 5 Pre dilution Sample Preparation Check oococcncccccnnoccccnnnoncnonancnnnnnocaninna na nnnnacannnnnrs 25 Checking the Hematology Analyzer sssssssssssssseeseeeeee nennen nennen nnne 2 7 Checking Background Noise isi scsi innit ia Le Eo Fue 2 7 Measurement sssssssssssssssseeeeee rene ee ren nnnnnnnn enne n nnne nennen nenne nennen 2 7 Service Manual MEK 6400 6410 6420 C 1 CONTENTS Parameter Data Check with Diluent sseeseeeeene 2 7 Reducing Background Noise eese nnne 2 8 Background Noise Problems ccccceeeeeeeeeeceeeeeeeeeeeneeeeeeeenean
18. PLT aggregation Measure the stained sample with a microscope Environmental Diluent temperature is low Environment temperature must be from 15 to 30 C condition is not Specified reagents are not used Only use the specified reagents appropriate Influence from other malfunctions High background noise Refer to the Background Noise Problems earlier in this section Measuring unit WBC sensitivity and threshold settings are WBC sensitivity must be 5 and threshold 4 unit failure failure inappropriate WBC aperture is not attached correctly Reattach the WBC aperture WBC aperture is dirty Clean the WBC aperture WBC aperture is damaged Replace the WBC aperture with a new one The screws securing the measurement Tighten the screws baths are loose The MC 640V measuring unit and chassis Remove the cause are not insulated there may be leakage The MC 640V measuring unit failure Replace the unit with a new one The UT 7198 MEASURING board failure Replace the board with a new one The hemolyzing Tube is in the upper part of the tank Put the tube in the bottom of the tank reagent Tube joints are loose Tighten the tube joint HEMOLYNAC3 Electromagnetic valve of the MD 640V Replace the unit with a new one is not dispensed combination syringe pump unit failure properly The MD 640V combination syringe pump Replace the unit with a new one The UT 7200 MIXED P
19. The DISPENSE key appears on the screen HOY 04 05 15 36 ID 0001 Co Press DISPENSE key to dispense diluent Mix the diluted blood thoroughly before measurement DISPENSE On MEK 6410 6420 On the READY screen select Pre dil for lt Dilute mode gt The DISPENSE key appears on the screen 2 Put the sampling nozzle into an empty cup and press the DISPENSE key on the screen Check that about 2 mL of diluent is dispensed into the cup ESULTS Checking the External Instruments Function Printers Check the printer function Check that data is printed properly and that the print is not faint and no dots missing Also check the auto print function Hand held Bar Code Reader Read a sample ID bar code label with the hand held bar code reader and check that the correct ID appears on the READY screen PC Send a sample data to PC and check that the data is properly received by the PC Checking the Power Cord Check that there is no damaged AC plug and exposed wire on the power cord Check that the 3 pin plug type power cord is used and the 3 pins and plug housing are not deformed Checking the Resistance of the Protective Ground Line Check that the resistance of the protective ground line of the power cord is 0 1 Q or less by using an earth tester or check the continuity with a multimeter Service Manual MEK 6400 6410 6420 6 45 6 MAINTENANCE Checking the Earth Leakage Current
20. Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the blood sample is high MCH and MCHC levels also become high Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually Excessive number of large PLT Excessive number of large PLT and or excessively high WBC may affect the MCV value Check by careful examination of the stained blood film MCH is determined from HGB and RBC values Therefore the limitations for HGB and RBC also affect MCH value MCHC is determined from HGB and HCT values Therefore the limitations for HGB and HCT also affect MCHC value RDW is determined from RBC value Therefore the limitations for RBC also affect RDW value Agglutinated RBC Agglutinated RBC may decrease RBC count and erroneous RDW This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Nutritional deficiency or blood transfusion Iron and or cobalamin and or folate deficiency may increase RDW Very small fragments Very small RBC RBC fragments and WBC fragments may be the cause in increased PLT count Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in decrease in PLT This can be checked by abnormal MCH and MCHC v
21. 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the cable connector from the POWER board blue and yellow cables To access the POWER board refer to Accessing the Connectors on the POWER Board earlier in this section POWER board Cable connector 4 Remove the cable connector from the photo sensor Remove all cable clamps from this cable Cable connector 5 Remove the screw and pull out the photo sensor Service Manual MEK 6400 6410 6420 4 17 4 DISASSEMBLY AND ASSEMBLY 6 Remove the 3 screws which secure the pump unit to the chassis The screws are guarded by blue rubber rings 7 Remove the 2 tube joints from the pump unit Tube joint Screw Screws 8 Pullout the pump unit toward you Reattaching the Pump Unit To reattach the pump unit reverse the above procedure 4 18 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the Sampler Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 R
22. 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the 3 cable connectors from the AMP CONTROL board The lowest cable is connected between the AMP CONTROL board and printer when the optional WA 640VK printer unit is installed in the instrument AMP CONTROL board Printer unit cable LIQUID SENSOR board POWER board AMP CONTROL board Cable connector 4 8 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Check the sampling nozzle position Make sure that the sampling nozzle is up and not inserted in the rinse unit If the sampling nozzle is lowered and in the rinse unit the sampling nozzle may be broken when the front panel unit is removed To raise the sampling nozzle to the top position rotate the upper coupling counterclockwise when viewed from the front E coupling Sampling nozzles Rinse unit There are 2 screws on the same position on the right side of the front panel 7 Carefully pull the front panel unit frontward while che
23. 9 Remove the measurement baths by pulling them toward ji c O Ab ji hi you to remove them from the aperture and then pulling e Lo them downward os b LD 10 Remove the cap from the left side of the WBC sub bath Ya LE See 1 mH i OI E Y br E 3 S 11 Remove the sub baths by pushing them to the left then 7 i pulling them toward you 12 Soak the measurement baths and sub baths in CLEANAC 3 detergent for about 10 minutes 13 Rinse the measurement baths and sub baths with water Filter joints Meas baths Sun and wipe them with a dry lint free cloth 14 Reattach the sub baths to their original positions Reattach the cap which was removed in step 10 to the left side of the WBC sub bath to fasten the sub baths Shaft 15 Reattach the measurement baths so that the sub bath is in the measurement bath the shaft of the sub bath is in the tab of the measurement bath and the round indent of the measurement bath fits the aperture 16 Tighten the screws which were loosened in step 8 to fasten the measurement baths Aperture NOTE Before tightening the screws check and remove any dirt or rust on and around the screws If dirt or rust is present noise alarm may occur during measurement Service Manual MEK 6400 6410 6420 6 25 6 MAINTENANC
24. Check that the earth leakage current is 0 5 mA or less under normal condition Check that the earth leakage current is 3 5 mA or less under each single fault condition 6 46 Service Manual MEK 6400 6410 6420 Section 7 Test Point Variable Resistor LED and Switch on Board AMP CONTROL lvi E 7 2 POWER Boald EEUU E EU o MU 7 4 MEASURING BO Arda a ia oido 1 5 FIGB AMP Board REMITTI 7 6 EIQUID SENSOR Board occiso ita iria iia 7 6 MIXED PUMP BOATO iii diet oi A Tf KEY BOG o er He LT PRINTER DRIVER Board ient eM cid 7 8 Service Manual MEK 6400 6410 6420 7 1 AMP CONTROL Board 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD 90 SO_ c ll 1N Q8 JTOYINO9 d O ARA A S 8619006 0619 NI0HOA NOH some e p boe H nod lea E ate ORLY Bw 1 ae E c e rf uei Seba z LA Dim rdg rag ad iid j E GPS A ees EA Eo ui MS L EI 85 Ea SE B hr q naita Viri NT c zm m mue fu CA mE E TE i any 58 a ra bu d Sony s LXXX EPEE d Tao ma suze na supe ce a ae sae eva FETTQTTTQTTEQEE QE Pree ere M LELELELE SL LHL BL Separa CIL DOG UGG E Mut ini ol a Eu t 2 a Tg tum un Wi ES A ee reer LL D D Ds Pi 2 2 e TAMTWOORN x wem cwm wee quer A wget og 22222535 fjr D n n n n n FEFERFERZ Y Mia TAEAE de ME Pal Service Manual MEK 6400 6410
25. LO A 4 Connect the detergent tube marked green to the CLN inlet on the right side panel Pass the detergent tube through the 18 L container cap Connect the end of the detergent tube to the cleanac tube assy Put the cleanac tube assy into the CLEANAC container and tighten the 18 L container cap CLEANAC 3 Detergent For STRONG CLEAN only 1 2 Cleanac tube 8 marked white MEK cap 3 Tube assy 6100 4 CLEANAC 3 detergent 5 in 2 L container Service Manual MEK 6400 6410 6420 Pour the CLEANAC 3 detergent into the 2 L container Use the 18 L container cock for pouring the diluent Connect the cleanac tube 8 marked white to the CLN inlet on the right side panel Pass the cleanac tube 8 through the MEK cap Connect the end of the cleanac tube 8 to the tube assy 6100 Put the tube assy 6100 into the 2 L container of the CLEANAC 3 detergent and tighten the MEK cap 9 5 9 INSTALLATION Lysing Reagent Tube Hemolynace3N 1 Replace the lysing reagent cap with the Hemolynac3 cap and tighten the cap 2 Connect the Hemolynac3 tube assy marked yellow to the HEMO3N inlet on the right side panel 3 Put the other end of the tube into the lysing reagent container through the Hemolynac3 cap Hemolynac3 tube assy marked yellow Hemolynac3 cap 4 When using the reagent tray place the lysing reagent container on the upper shelf of the reagent tray Lysing reagent Connec
26. OR WITH OXYGEN OR NITROUS OXIDE EQUIPMENT not suitable for use in the presence of FLAMMABLE ANAESTHETIC MIXTURE WITH AIR OR WITH OXYGEN OR NITROUS OXIDE According to the mode of operation CONTINUOUS OPERATION EQUIPMENT types classification Indoor stationary EQUIPMENT Pollution Degree 2 EQUIPMENT Requirements for marking of IN VITRO DIAGNOSTIC instruments EN1658 1996 1 6 Service Manual MEK 6400 6410 6420 1 GENERAL Panel Description Front Panel VA NIHON KOHDEN CellttacK VA NIHON KOHDEN CellttacK 4 8 7 9 10 1 2 0 eo B 11 d 4 5 6 Y 12 gt 13 15 it L 14 16 E MEK 6400 MEK 6410 6420 No Name Description 1 Main power lamp Lights when the Main power switch on the rear panel is tuned on 2 Power lamp Lights when the Main power switch on the rear panel and Power key on the front panel are turned on Turns the analyzer power on or off when the Main power switch on the rear panel is turned 3 Power key on When the power is turned on priming and self check are automatically performed and the READY screen appears 4 Auto print key Switches the printing mode between automatic and manual for the printer 5 Feed key
27. There are no damaged or dirty parts on the outside of the analyzer The reagents are not expired There is no leakage from the analyzer The sampling nozzle switches and keys are not damaged The labels are not torn or removed The reagents are properly connected to the analyzer The tubes are not damaged bent or clogged The power cord is connected properly The power cord is not damaged Grounding lead 1s connected properly The external instruments are properly connected to the analyzer The connection cables are not damaged No alarms appear when the analyzer is turned on and the READY screen appears The messages are displayed properly The touch screen keys function properly Calibrate the touch screen when necessary Date and time are correct Enter the value Closed Open WBC 0 2 x107 uL Measure background noise RBC 0 05 x10 uL HGB 0 1 g dL PLT 10 x10uL Measure hematology control and check that the obtained data is within the acceptable range on the assay sheet of the hematology control Check that the measurement baths and sub baths are not dirty or damaged Check that the pump tube is not collapsed or damaged Every 300 counts Check that filters and filter packings are not damaged Monthly or every 1 000 counts whichever comes first Replace filters Clean sub baths and measurement bath
28. This message appears when the manometer upper or lower sensor detects no liquid during RBC measurement or at the start of draining manometer Reason Possible Cause Criteria Countermeasure Diluent cannot be detected even when there is enough RBC manometer is dirty Perform strong cleaning RBC manometer upper sensor adjustment error Adjust the sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in diluent LED does RBC manometer lower sensor adjustment Section 5 not light error The MC 640V measuring unit failure Replace the unit with a new one Air bubbles in the RBC manometer is dirty Perform strong cleaning manometer RBC aperture is not attached properly Reattach the RBC aperture with a new one The MC 640V measuring unit failure Replace the unit with a new one A045 RBC bubble 2 This message appears when the manometer upper sensor detects the liquid during RBC measurement or when draining manometer from upper sensor OFF to lower sensor OFF Reason Possible Cause Criteria Countermeasure Air bubbles in the RBC manometer is dirty Perform strong cleaning manometer The MC 640V measuring unit failure Replace the unit with a new one A046 RBC bubble 3 This message appears when the manometer lower sensor detects no liquid during RBC measurement or aspirating sample from lower sensor ON to
29. Threshold settings for panic value FLAG SETTINGS Threshold settings for special flag displa CED om BAR CODE PORT Settings for RS 232C communication INPUT UNIT CAL Compensation value settings CL HPUT UNIT CAL ADV SETTINGS Screen The following settings are available TT 82 EE RM HOY 04 05 16 21 Clean after 10th measurement Y on O ort Clean after 10 measurement High altitude Automatically cleans the fluid path with the detergent after every 10 O on Y ort measurements when this setting is set to on To cancel this function set this Sa ee ERES setting to off This setting is only for MEK 6400 and MEK 6410 O on A ort High altitude Maia Makes the measurement time longer when this setting is set to on When the ZF on Q ort instrument is installed at a place lower than 1000 m above sea level check that MEER this setting is set to off O On Y ort Using handy bar code in measurement Allows input of sample ID number with a handy bar code reader when this setting is set to on Histogram smoothing Smooths the histogram when this setting is set to on The default setting is on Service Manual MEK 6400 6410 6420 2 35 2 TROUBLESHOOTING HOY 04 05 16 21 MANAGE REAGENT FACTOR HOY 04 05 16 21 Filters baths o z 1000 Pump tube 0 2000 Rinse unit 0 1000 Sanpling nozzle 0 3000 Cap pierce nozzle 0 1000 OK 2 36 Alarm recou
30. can be produced but there is leakage RBC aperture is not attached properly Reattach the RBC aperture properly The MC 640V measuring unit failure Replace the unit with a new one Measuring at high altitude higher than 1 000 m above sea level High altitude mode needs to be set to On Set High altitude on ADVANCED SET SETTINGS MENU of the SERVICE screen must be set to On 2 22 Service Manual MEK 6400 6410 6420 A050 RBC sample error 2 TROUBLESHOOTING This message appears when the voltage between the electrodes is out of acceptable range Reason Possible Cause Criteria Countermeasure Diluted sample error The diluent temperature is too low The environment temperature must be from 15 to 30 C for RBC count Specified reagents are not used Only use the specified reagents A051 RBC noise 2 This message appears when time sequence of the counted pulses is not stable A052 PLT noise 1 This message appears when there is baseline wandering during measurement A053 PLT noise 3 This message appears when the pulses are smaller than the threshold of counted pulses Reason Possible Cause Criteria Countermeasure Power line 2 pin power cord is used Use 3 pin power cord Ground lead is not connected Perform equipotential grounding Decrease in the power voltage Do not share the power source with any other instrument
31. 16 21 operation done by MS 640V sampler unit So you can check the fluid level movement and any bubble in the two manometers without any noise and vibration from the other units in the instrument UNIT MEAS X5 Automatically measures each sample in the WBC and RBC measurement baths 5 times separately from dilution operation done by MS 640V sampler unit So you can check the data reproducibility without any noise and vibration from the other units in the instrument WBC UNIT MEAS Measures the sample in WBC measurement bath separately from dilution operation done by MS 640V sampler unit RBCUNITMEAS Measures the sample in RBC measurement bath separately from dilution operation done by MS 640V sampler unit PRIME BATH Fills the two measurement baths with diluent PRIME UNIT Fills the two manometers with diluent CLEAN UNIT Cleans the two manometers with detergent BACKUPRAM CHK No function UNIT MAINTE Screen HOY 04 05 16 21 This screen allows you to check the operation of the following units and valves amm aM PMS 641Y MY amp PUMP CHK MD 640V Combination syringe pump unit MS 640V Sampler unit MS 641V Cap pierce unit MP 640V Pump unit Solenoid valves OK 2 32 Service Manual MEK 6400 6410 6420 HD 640 aay HOY 04 05 16 21 TD a DISPENSE A _ __ ___ a 5 TIMES AUTO PRIME itl OK HOY 04 05 16 21 IHITIALIZE ASPIRATE POS PIERCE POS WB
32. 30 C 59 to 86 F Operating humidity 30 to 8596 Operating atmospheric pressure 70 to 106 kPa If the diluent or hemolysing reagent temperature is lower than 15 C it will affect the measurement data of HGB WBC and WBC differential parameters or cause a hemolysing error or sample error alarm Blood Sample Handling Check Check that the blood sample is counted immediately after collection or stored in a cool place at temperatures between 2 and 8 C 36 and 46 F such as a refrigerator If the blood sample is stored in a refrigerator at temperatures lower than 2 C for a long time or left more than 12 hours at room temperature after collection the WBC differential parameters may be affected NOTE If the blood sample causes hemolysing error when it is counted within 30 minutes after collection wait at least 30 minutes after collection before counting it Measurement data might not be accurate for special blood sample such as from a neonate or patient with hepatic disease or special treatment In most cases these blood samples are difficult to hemolyze because RBC have strong membrane resistance and the analyzer counts high WBC Bilirubin or WBC in blood affects MCHC These special blood samples need a different measurement method Pre dilution Sample Preparation Check Most measurement data errors in pre dilution mode are caused by blood clotting and dilution of the blood sample Therefore check the following points Whe
33. 50 samples Environmental Conditions Storage temperature 20 to 60 C Operating temperature 15 to 30 C Storage humidity 10 to 95 Operating humidity 30 to 85 Non condensing Storage atmospheric pressure 70 to 106 kPa Operating atmospheric pressure 70 to 106 kPa Operating altitude less than 3000 m Power Requirements Power requirements MEK 6400J 6410J 6420J 110 to 127 V 10 AC 50 60 Hz MEK 6400C K 6410C K 6420C K 220 to 240 V 10 AC 50 60 Hz Power consumption less than120 VA Service Manual MEK 6400 6410 6420 1 5 1 GENERAL Dimensions and Weight Dimensions 230 W x 450 D x 383 H mm Net weight approx 18 kg Electromagnetic Compatibility IEC 61326 1 Edition 1 0 2002 Annex A EN 61326 1997 Amendment 3 2003 CISPR11 Edition 4 1 2004 Group 1 Class B EN 55011 1998 Amendment 1 1999 Group 1 Class B The power supply short interruption test is performed through the transformer which has at least three times the power capacity as the instrument Safety Safety standards IEC 61010 1 Edition 2 0 2001 EN 61010 1 2001 IEC 61010 2 101 2002 EN 61010 2 101 2002 IEC 61010 2 081 2002 EN 61010 2 081 2002 According to the type of protection against electrical shock CLASS I EQUIPMENT According to the degree of protection against harmful ingress of water IPXO Ordinary EQUIPMENT According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR
34. 6 MAINTENANCE 4 Slide the sampling nozzle plate to the right Sampling nozzle plate ati D TRUM S WT RC ccm c a y TO iD qe d Epid SR oh gt O We ET gt E AUS a 5 Check the following parts for dirt or blood clot Remove blood or salt crystals on the tip of the sampling nozzles with a cotton swab or lint free pad moistened with CLEANAC 3 detergent F 4 rig Jd a 97 LAE Tx 1 i b Sampling nozzles If the sampling nozzle is damaged or dirt blood cannot be removed replace Sampling nozzle plate the sampling nozzle with a new one should be about here To replace sampling nozzles x H gf 1 Push down the sampling nozzle plate as far as it goes The sampling i nozzle plate must be at the position indicated at left Otherwise the L A measurement baths and sub baths get in the way and the sampling i ia nozzles may be damaged 6 30 Service Manual MEK 640
35. 6 13 Turning Power Off scaring iaai nnne nn ranma 6 14 Daily Maintenance Procedures ocooonccccnnnccnocncccnnnnnnncnnncnnnnnnnnnnn R 6 15 Checking Reagents and Other Consumables sss 6 15 Checking the Appearance of the Analyzer ssssssseeee 6 15 Service Manual MEK 6400 6410 6420 C 5 CONTENTS Disinfecting the Surface of the Analyzer ssssssssene 6 16 Cleaning the Surface of the Analyzer sssssssssseeee 6 16 Checking the Reagents Connection Tubes sssssssseneeen 6 16 Checking the Power Cord and Grounding Lead sssseeee 6 16 Checking the External Instrument Connection sss 6 16 Checking the Power On ooccccccoccccnonccccononcnnanancnnnnoncnnnno cnn nano nennen enne enne nnn nnn 6 17 Calibrating the Touch Screen sssssssseeseeeeennen 6 17 Checking the Date and Time cuidar lc 6 17 AL nennen nnnm e nnne nnt rne nnns nnns 6 18 Measuring Background NoISG 2 reiner os 6 18 Measuring Hematology Control ooooconncccccnoccccnonncccnonancnncnnaca conc cn conan corran ea 6 20 Checking Measurement Baths and Sub Baths oooconoccccnnccccnnnccccnonannncnnnananana nc 6 21 Checking Pump TUD6 oii arica iaa 6 21 Weekly Every 300 Counts Maintenance Procedures cooooocccccccconoconccnncoononnncnnnconnnnnonnnnos 6 21 Checking Cleaning Filters eesssesesesesseeeeeee nennen nenne
36. 6420 7 2 TP011 5V TP012 ED TP013 3 3V TPO14 4 Vp TP015 Ep TP016 M15V TP017 EM15V SWO0141 TP018 15V TP019 EA TP0110 15V TP031 RFIL TP032 RIN TP033 R GATE TP034 R ROB TP035 RCLLEV TP036 RELE TP041 RANA TP042 R PH TP043 R THR TP044 R PLS TP051 WFIL TP052 WIN TP053 W GATE TP054 W ROB TP055 WCLLEV TP056 WELE TP061 WANA TP062 W PH TP063 W THR H TP064 W PLS H TP065 W THR L TP066 W PLS L TP071 HGB IN m oc LE 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD 5 V DC terminal for digital circuit Ground terminal for digital circuit 3 3 V DC terminal for digital circuit Supply voltage Vp terminal for optional built in printer Ground terminal for optional built in printer 15 V DC terminal for valve control Ground terminal for valve control Bit switches for debug All the bit switches are set to off 15 V DC terminal for analog circuit Ground terminal for analog circuit 15 V DC terminal for analog circuit Test point for RBC pulses through the low pass filter Test point for RBC pulse analog waveform from the measuring unit Test point for RBC gate pulse Test point for output waveform from Robinson gate circuit for RBC Test point for RBC level Test point for voltage between the electrodes at RBC side Test point for RBC analog output waveform from the gain setting circuit Te
37. Count switch on the front panel The hematology control is aspirated and counting is performed After counting the result appears on the screen Service Manual MEK 6400 6410 6420 6 MAINTENANCE 7 Check the data with the assay sheet NOTE Calibrate the analyzer when the obtained sample data is different from the values described on the assay sheet after recounting For details refer to Section 7 Calibration in the Operator s Manual Checking Measurement Baths and Sub Baths Refer to the Checking and Cleaning Measurement Baths and Sub Baths in the Monthly Every 1 000 Counts Maintenance Procedures later in this section Checking Pump Tube Refer to the Replacing Pump Tube in the Every Four Months Every 3 000 Counts Maintenance Procedures later in this section Weekly Every 300 Counts Maintenance Procedures Checking Cleaning Filters Check and clean the filters once a week or every 300 sample counts Refer to the Replacing Filters in the Monthly Every 1 000 Counts Maintenance Procedures later in this section Service Manual MEK 6400 6410 6420 6 21 6 MAINTENANCE Monthly Every 1 000 Counts Maintenance Procedures Replacing Filters Materials Required Powder free gloves lab coat safety glasses Phillips screwdriver Tweezers Procedure Replace the filters when they are clogged dirty and or after every 1 000 sample counts 1 Do the procedure in the
38. External Electrodes on the Measurement Baths 6 39 Checking the Sensor Monitor Screen ocococnocccinocccccoooccnnnnaccconnn conca cnn n nana nanannn cnn 6 40 Checking the CIreult assi adi usd 6 41 Checking the Aa 6 43 Checking the Current Calibration Coefficients sseeeeee 6 43 Checking the New Calibration Coefficients oonmnocinnnnnn ncccnnnnnccnnncccncnnnananinancnnn 6 43 Checking the Prime Function ooooocccnncccccnonocanonannncnananoncnnoco conan nn cnn ncnn nano canaria edia 6 44 Checking the Drain Function sessesessssseseeeneeeeennnene nennen nennen 6 44 Checking the Cleaning Function ssssssssessseeeeeeneeneen nennen 6 44 Checking the Dispensing Function cooococcnnncccnnonaccnonocnnnnnnn conan o canon cnn cnn conan conos 6 45 Checking the External Instruments Function oooccconccccnnncccconancccnnnanannanananannn nana 6 45 ilc ge ss E T T T E T A 6 45 C 6 Service Manual MEK 6400 6410 6420 CONTENTS Hand held Bar Code Reader cooooocccccccoooocccccccconoonnnnnncconannncnnnncnonannncnnnccinnnns 6 45 Ho o 6 45 Checking the Power Cord scooter 6 45 Checking the Resistance of the Protective Ground Line ssueuuses 6 45 Checking the Earth Leakage Current sssssssseeeeeeneennene 6 46 Section 7 Test Point Variable Resistor LED and Switch on Board 7 1
39. Feeds paper of the printer while held down 6 Print key Prints displayed data on the printer 7 jus print iede Lights when automatic printing mode is selected 8 LCD display Displays various messages measured data and touch screen keys Stops operation when pressed during operation Returns to the READY screen when pressed 9 Reset key j while changing settings Use this key only when an error occurs Cleans the fluid path aperture and manometer with detergent Automatically primes after 10 Clean key cleaning the fluid path Press this key when clogging occurs the manometer becomes dirty or bubbles occur in the manometer Eject key For closed mode only Opens the tube holder to set the sample tube MEK 6400 Count k Aspirates the sample and starts counting when lt Eject key operation gt is set to Count on ji 2 ey the OPERATION screen of the SETTINGS screen MEK 6410 MEK 6420 Dispense ke Dispenses the diluent in pre dilution blood mode when lt Eject key operation gt is set to spense Soy Dispense on the OPERATION screen of the SETTINGS screen MEK 6410 MEK 6420 12 Tube holder For closed mode only Holds a sealed vacuum blood collecting tube Press the Eject key to open After measurement the holder automatically opens MEK 6400 Aspirates the sample Dispenses the diluent when in the pre dilution blood mode On MEK 13 Sampling nozzle 6400 for open mode only 14 Count switch Aspirates the sample an
40. MAINTENANCE Checking the Sensor Monitor Screen HOY 04 05 16 21 E CALIBRATION SETTINGS d DATA OPERATIONS HOY 04 05 16 40 gm aA OF RAO CIRCUIT CHEGI MAINTENANCE GD GE BACKGROUND 6 40 You can view the power voltage for the sensors in real time on the SENSOR MONITOR screen For the upper and lower manometer sensors the voltage must be checked without reagent and then with reagent in the manometers Before displaying the SENSOR MONITOR screen make sure that the fluid are drained from the instrument 1 Press the OTHER key on the MENU screen to display the OTHER screen 2 Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen appears listing the output voltage of each sensor 1 8824 1 995 1 8954 Temp compen RBC 0 01V lt Mano Reagent gt WBC manometer RBC manometer With reagent 1 5V Without reagent gt 3 5 Y 27 0 C 36 4 C 32 4 C 3 Check that the results are in the following ranges Normal range lt Electrode gt 1 HGB LED On 1 5to 4 5 V 2 HGB LED Off 0 5 V 3 WBC 17 7 to 18 3 V 4 RBC 17 7 to 18 3 V lt Mano Reagent gt Without reagent in the manometers 5 WBC manometer upper gt 3 5V 6 WBC manometer lower gt 3 5V 7 RBC manometer upper gt 35V Service Manual MEK 6400 6410 6420 6 MAINTENANCE 8 RBC manometer lower gt 3 5 V 9 Diluent 23 5V 10 Lysing reagent gt 3 5V lt Temperature gt
41. MENU key on the READY screen The MENU screen appears 2 Press the SETTINGS key on the MENU screen The SETTINGS screen appears 3 Press the USERS key on the SETTINGS screen The USERS screen appears Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING HOY 04 05 14 32 4 Select FACTORY and press the CHANGE key A promt for the password Name Type of user factory Service gt Lab technician Other user Other user uv 5 Type 4321 and press the ENTER key 6 Press the OK key on the screen The SETTINGS screen appears CHANGE ADD USER OK DELETE 7 Press the OK key on the screen The MENU screen appears HOY 04 05 16 21 8 Press the OTHER key on the screen The OTHER screen appears em emm CALIBRATION SETTINGS DATA OPERATIONS 9 Press the SERVICE key on the screen The SERVICE screen appears The SERVICE screen has the following menu items HOY 04 05 16 21 CHECK MENU UNITMAINTE CU QED coer easure TWIT MAINTE SETTINGS MENU CONT MEASURE MONITOR SETTINGS MEN rai INITIAL MENU m oen 510 CV PRINTSETTINGS FTHITIAL WENU GI OKR Service Manual MEK 6400 6410 6420 2 31 2 TROUBLESHOOTING CHECK MENU Screen This screen allows you to check the measurement operation of the following blocks WBC amp RBC UNIT Simultaneously measures each sample in the WBC and RBC measurement baths separately from dilution HOY 04 05
42. Operator s Manual 3 Remeasure the hematology control Confirm that the obtained sample data is within the acceptable range on the assay sheet attached to the hematology control Poor WBC Reproducibility The following may be the main reasons for poor WBC reproducibility Measuring unit and fluid path are dirty Reagent is deteriorated Samples are not stirred enough Hematology analyzer failure Measurement baths rinse unit or fluid path is dirty Possible Cause Criteria Countermeasure WBC measurement bath is dirty Press the Clean key on the front panel to perform cleaning Perform strong cleaning Clean the WBC measurement bath Replace the WBC sub bath with a new one Replace the WBC measurement bath with a new one Drain path of WBC measurement bath is dirty Replace the hemoglobin filter below air trap with a new one Clean the air trap with a cotton swab Rinse unit is dirty Clean the rinse unit WBC aperture is dirty Clean or replace the WBC aperture with a new one Deterioration of reagents Possible Cause Criteria Countermeasure The specified reagents are not used Use only the specified reagents Deterioration of reagents Replace the reagents with new one Use the reagents in appropriate conditions The reagents are used outside the specified temperature range Use the reagents in the appropriate temperature Service Manual MEK
43. Pierce Nozzle ooooooccccccconoccccncconanancnnnnnonnna 6 26 Materials ROQUING E 6 26 PFOCCQUIC e MR 6 27 Every Four Months Every 3 000 Counts Maintenance Procedures ssssssssseeeneeeneeeenneeeenn 6 29 Checking and Cleaning Replacing the Sampling Nozzles oooooonococcnncccccnocccconnonccnonananonnnccnannn cnn nana ca nn 6 29 Materials Requintos io ei oi epoca 6 29 Proc oduna a ua sentesea E S E O cen fain wesc tones tate t wu hagtansaceseanatne 6 29 Replacing PUMP TUDE e 6 32 MatenialS REQUIFOG mucosa ra RE 6 32 diee me EET 6 32 Service Manual MEK 6400 6410 6420 6 1 Every Six Months As Required Maintenance Procedures oococcccoccccnooccncononcnnonnnnononnncnnonn cnn nono nnnnnnn nn rnnnn na canencanans 6 35 6 2 Removing a Clogifrom the Aperture isisingit aaa eE Aus ccu dba kun dera NH EROR RRzR SERRE 6 35 Cleaning Aperture Caps tin dee dec deerit Hep reb DI REC ciere khe cutem t E ecc PR ee teet feta 6 36 Materials REQUIRCG 22 e EE 6 36 POCOS ui os 6 36 Cleaning the External Electrodes on the Measurement Baths 0ococonociconiccccnnoccccnonnncnnnnancnnnnn nro nano narran 6 39 Checking the Sensor Monitor Screen oooocconnccccnnocccconannnnnonocanonnn nc nono n corran cana nn nc rn nr rra nennen enne 6 40 Checking the Circuit Soie aia ad a a 6 41 Checking the XR Vallas areas 6 43 Checking the Current Calibration Coefficie
44. a new one A062 HGB voltage high This message appears when the HGB BLANK diluent voltage is more than 4 5 V or the hematology analyzer was drained Reason Possible Cause Criteria Countermeasure Deterioration HGB voltage adjustment error Adjust the HGB voltage Refer to Adjusting the from age HGB Sensor Output Voltage in Section 5 Erroneous HGB voltage is adjusted when there was After cleaning adjust the HGB voltage operation blood sample in the WBC measurement bath Circuit error The UT 7201 HGB AMP board failure Replace the board with a new one The UT 7202 HGB LED board failure Replace the board with a new one The UT 7198 MEASURING board failure Replace the board with a new one The MC 640V measuring unit failure Replace the unit with a new one A063 HGB circuit error This message appears when the voltage is more than 0 5 V at HGB LED off Countermeasure Reattach the HGB cover Replace the board with a new one Replace the board with a new one Replace the board with a new one Replace the unit with a new one Possible Cause Criteria The HGB cover is not attached properly The UT 7201 HGB AMP board failure The UT 7202 HGB LED board failure The UT 7198 MEASURING board failure The MC 640V measuring unit failure Reason Light interference Circuit error A072 Tube holder open The analyzer cannot operate because the tube h
45. an anticoagulant OTHER FLAGS This message appears when the CBC parameters are outside the range Reason Possible Cause Criteria Countermeasure Adjustment and calibration are not appropriate Calibration for CBC parameters is not appropriate Perform calibration Environmental condition is not appropriate Specified reagents are not used Only use the specified reagents The environment temperature is out of 15 to 30 C The environment temperature must be within 15 to 30 C Service Manual MEK 6400 6410 6420 2 27 2 TROUBLESHOOTING Solving Problems from System Error Messages E001 DILUTER INITIALIZE ERROR The MD 640V combination syringe pump unit cannot be initialized Reason Possible Cause Criteria Countermeasure Diluter piston failure unit is damaged Cable s on the MD 640V combination syringe pump Replace the cable with a new one Motor driver on the UT 7193 POWER board failure Replace the board with a new one unit failure Motor on the MD 640V combination syringe pump Replace the unit with a new one diluter piston Sensor cannot detect the movement of the The UT 7200 MIXED PUMP board sensor failure Replace the board with a new one unit damaged Cable s on the MD 640V combination syringe pump Replace the cable with a new one E021 SAMPLER INITIALIZE ERROR The MS 640V sampler
46. and a fluid path to send the diluent or detergent to the other units Service Manual MEK 6400 6410 6420 3 7 3 BOARD UNIT DESCRIPTION MS 640V Sampler Unit This unit moves the two sampling nozzles to the positions for aspiration and dispensing The diluted sample is dispensed into the WBC and RBC measurement baths by coordination between this sampler unit and the syringe pump unit MS 641V Cap Pierce Unit MEK 6400 only This unit pierces the cap of a sample tube with a cap pierce needle to aspirate the blood sample with the sampling nozzle without opening the cap of the sample tube This unit also has a cap pierce rinse unit to clean the cap pierce needle 3 8 Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION MR 640V Rinse Unit MEK 6410 6420 only This unit cleans the sampling nozzles UT 7192 AMP CONTROL Board This board consists of two blocks One block amplifies the analog signals from the measuring unit and converts the amplified analog signals to digital signals The other block controls the valves and motors while receiving the key switch status and signals from the sensors This board controls all the operations such as cleaning and measurement Service Manual MEK 6400 6410 6420 3 9 3 BOARD UNIT DESCRIPTION UT 7193 POWER Board This board converts AC line voltage to the necessary DC voltages through the power transformer This board also has all the motor driver ICs t
47. and fasten it with the three screws on the rear panel and one screw on the right side panel 8 Press the Power key to turn on the power The analyzer starts priming the fluid pathway 9 If filters were replaced reset the filter counter When the filter counter is OPER HISTORY P WO 04 05 16 40 04 05 16 40 Filters baths 8 sub baths reset the measurement baths and sub baths counter is also reset Before resetting the counter the measurement baths and sub baths should be 83 1000 sx 83 3000 Pump tube cleaned Refer to the Checking and Cleaning Measurement Baths and Sub RESET 83 4 1000 is Sampling nozzle Cap pierce nozzle 61 4 1000 A 33380 Baths section Rinse unit To reset the counter press the RESET key for Filters baths amp sub baths on the OPER HISTORY screen to reset the counts to zero 10 Fill in the Maintenance Check Sheet AAA Checking and Cleaning Measurement Baths and Sub Baths Check the measurement baths and sub baths every day Clean the measurement baths and sub baths when there is any blood or dust on them Once a month or every 1 000 sample counts Materials Required Powder free gloves lab coat safety glasses Phillips and flat blade screwdrivers e CLEANAC 3 detergent Dry lint free cloth Service Manual MEK 6400 6410 6420 6 23 6 MAINTENANCE Procedure NOTE Be careful not to damage the measure
48. board with a new one The reagent is dirty Replace the reagent with a new one Checking the Reproducibility This check is used to check reproducibility of the instrument using printout of the CV values of 10 data of a diluted sample of the same MEK 3DN hematology control When the CV values are out of the specification range the reproducibility of the instrument is poor If the reproducibility is found to be poor this printed result is used to troubleshoot the instrument Checking Procedure 1 Reduce the background noise 2 Warm the sample MEK 3DN to room temperature 15 to 30 C 59 to 86 F and stir it sufficiently 3 Measure the sample 10 times Service Manual MEK 6400 6410 6420 2 9 2 TROUBLESHOOTING 2 10 4 Press the MENU OTHER SERVICE gt X10 CV key to display the result and check the CV data The type of user must be Service to enter the SERVICE screen CV Values The reproducibility is checked using the CV values The CV value is acquired by dividing the standard deviation by the average value When the CV value is large it indicates that the measurement data variability is large 1 e reproducibility is poor Example Parameters Measured Value Acquired CV Standard CV average WBC 6 8 x10 uL 1 3 2 0 LY 5 0 MO 12 0 GR 5 0 RBC 4 43 x10 uL 0 8 1 5 HGB 13 0 g dL 0 7 1 5 MCV 91 fL 0 4 1 0 PLT 242 x107 uL 32 4
49. code reader socket Power supply voltage 5 V DC pin 9 5 V pin 5 GND Rated current 200 mA 6 Serial port 1 Connects to the optional WA 460V 461V card printer or PC 7 Serial port 2 Connects to the optional WA 460V 461V card printer or PC 8 Printer socket Connects to an external printer WA 710V 712V or other 9 USB socket Connects to a PC The optional Data Management Software needs to be installed on the PC to receive data from the analyzer 10 Memory card socket Insert a memory card when you upgrade the software 11 Memory card eject button Press this button when you eject a memory card Service Manual MEK 6400 6410 6420 1 9 1 GENERAL Composition MEK 6400 Standard MEK 6400J MEK 6400K MEK 6400C MC 640V Measuring Unit UT 7198 MEASURING Board UT 7201 HGB AMP Board UT 7202 HGB LED Board XP 612V 2 Way Electromagnetic Value x 10 XP 602V 2 Way Electromagnetic Value x 2 MD 640V Combination Syringe Pump Unit UT 7200 MIXED PUMP Board XP 612V 2 Way Electromagnetic Value x 4 MP 640V Pump Unit PV 641VK Front Panel Unit UT 7203 KEY Board JQ 640V Inlet Outlet Unit UT 7199 LIQUID SENSOR Board XP 612V 2 Way Electromagnetic Value x 2 Valve Unit XP 612V 2 Way Electromagnetic Value x 6 Sampler Unit Cap Pierce Unit AMP CONTROL Board POWER Board Option Printer Unit PRINTER DRIVER Board 1 10 Service Manual MEK 6400 6410 6420 MEK 6410 Standard MEK 6410J MEK 6410K MEK
50. dae ga 4 36 Removing the LIQUID SENSOR Board sse eene 4 37 Reattaching the LIQUID SENSOR Board sse 4 37 Removing the LCD erisir ainan ennaa aad aade nennen nennen nennen enn ASTMES AE ennt enn rne nnns 4 38 Reattachingithe ECD cata E 4 39 Removing the TransfOrtmer tii dd Eon pd Da uu d 4 40 Reattaching the Transformer sssseeseeeeennnmeenn menn 4 40 SECTIONS A a EM lande 5 1 erg c ENAN 5 2 Adjusting the HGB Sensor Output Voltage sssssssseeeeeeneennenn 5 3 Adjusting the Upper and Lower Sensor Output Voltages of the Manometers 5 6 Adjusting the Liquid Sensor Output Voltages ssseseeeeeneeenn 5 9 Section 5 Maintenance eot nta 6 1 Ec e ria A E P AEE ERE OEE AEE E N SE EET EEE 6 3 DISPOSING Of Waste vir oa 6 4 Repair Parts Availability Policy ooonnnoncccnnnnnnccccccccnonananccccnnnnnannccnnnnnnanancnnccnnnnns 6 4 Parts to be Replaced Periodically eese 6 4 Maintenance Schedule uc eben reae Ceres t Fire tea a deett deseas ss 6 5 Displaying Operation History Screen sssssssssseseeeeneeeen nennen 6 6 Maintenance Check Sheet issnin oinin annaia aaan aa EAEE Naaa 6 8 Betore Maintenance Procedure iret a EE 6 12 Strong Cleanihg 2c sated E 6 12 Draining Measurement Baths and Sub Baths ccccceeeeeeeeeeeeeeeeeeeeeeteeeeees
51. earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the front panel unit Refer to Removing the Front Panel Unit earlier in this section 4 Place the front panel unit face down on a table covered with a clean soft and smooth antistatic material NOTE If the front panel unit is not faced down the key top covers will come off and fall from the rear of the front panel cover 5 Remove the 4 screws and pull out the front panel chassis from the front panel cover Front panel chassis Front panel cover 4 38 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 6 Remove the 4 screws from the front panel chassis Flat cable Cable connector Pink and white cables Touch screen cable Screw Tape Carefully peel off the 3 pieces of tape which fasten the flat cable and pink and white cables to the front panel unit 8 Remove the cable connector flat cable connector and touch screen cable connector and put the front panel unit face up o Carefully pull out the LCD from the front side of the front panel chassis Reattaching the LCD To reattach the LCD reverse the above procedure Service Manual MEK 6400 6410 6420 4 39 4 DISASSEMBLY AND ASSEMBLY Removing the Transformer WARNING For your safety before dis
52. enne nennen ennnnr sent rnn sent nne sen trns seines enne rennen nnns 3 9 UWT7193 POWER Board c M 3 10 1T7198 MEASURING BOA m 3 10 UT 7201 HGB AMP Board and UT 7202 HGB LED Board cooococccnccccccnoccccononcnnonnncononnncnnonnnnanannn no nann ana nnnnn ca nannnnanns 3 11 UT 7200 MIXED PUMP Board Y 3 11 UT 7205 PRINTER DRIVER Board cococccccoccccconoccnononcnononancnnononnnnonnnnn nan nn aran nr nn an iiaeia baide aganida iiaae 3 12 UT 7208 KEY Board iecore eoe te gestes aia in 3 12 UT 7199 LIQUID SENSOR BOANA 2 2 etta edes ee reve pete c tona lacio pia 3 13 XP 602V XP 612V 2 way Electromagentic Valve ooocccinnccccincccccononccnonancnnonnn canon o conan nennen nennen enne nnn nennen 3 13 Service Manual MEK 6400 6410 6420 3 1 3 BOARD UNIT DESCRIPTION 3 2 This section describes the following units and boards and how they work during operation This information helps you to fully understand the overall operation of the instrument Measuring unit Combination syringe pump unit Pump unit Front panel unit Inlet Output unit Valve unit Sampler unit Cap pierce unit MEK 6400 only AMP CONTROL board POWER board MEASURING board HGB AMP board and HGB LED board MIXED PUMP board PRINTER DRIVER board KEY board LIQUID SENSOR board 2 way electromagentic valve Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION Board and Unit Location Right Side View Va
53. nnne nenas 2 24 A062 HGB voltage high PET Adie i reise el a eda sad 2 24 A063 AGB Circuit GEFOE 2 2 2d iata cirea pei io aaa 2 24 A072 Tube holder oper iii is 2 24 A073 Tube in the holder 2 25 A091 Room temperature high occooocooccccncnoncconccncnonanonononcnnnnnonononcnnnnnn nn nc nc rn nan nn nr rr cr nan nnne nennen nne 2 25 A092 ROOM temperature lO Wisin alicia 2 25 A093 Internal temperature DIGA score 2 25 A094 Internal temperature low sess eene nnne eene n nnne enne 2 25 A095 Power Supply temp Nighi uiuere redeo cete tenera endo ek O 2 25 APPEARS ON THE RIGHT OF WBC MEASURED VALUE seen 2 26 APPEARS ON THE RIGHT OF MCHC MEASURED VALUE esee 2 26 C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or PLT Clumps appears 2 27 Sg FLAGS ass mE 2 27 Solving Problems from System Error Messages ooccccccocccccooccconancnonononcnconannnnnnn cnc nano cnn nan nn nr nan n rr nan eran entren nennen 2 28 E001 DILUTER INITIALIZE ERROR 3t netten ta t pe da ine 2 28 E021 SAMPLER INITIALIZE ERROR sss eene enne nennen nennt enne nn 2 28 E041 SUB BATH INITIALIZE ERRBOR 2 2 2 1 id bea 2 28 E101 BATHIDRAINIEBBOPN ier do ende tex rl etapa a gesu Ve year its 2 29 E122 CHECK SETTINGS initiates eter rene ia 2 29 E123 MEMORY ERROR terra a eu fh anh aes 2 29 E124 CIRCUIT ERROR iui deidad lili 2 29 E126 TOUGH PANEL ERROR coccion sia 2 2
54. of noise The reagents are not used in the appropriate Use the reagents in the appropriate temperature temperature range and they are deteriorated range High background The filters are clogged or dirty Clean or replace the filters with new ones noise of WBC The measurement baths and sub baths are Clean or replace the measurement baths and sub RBC and PLT dirty baths with new ones The apertures are dirty Clean or replace the apertures with new ones The rinse unit is dirty Clean the rinse unit The apertures are damaged Replace the apertures with new one The MC 640V measuring unit is damaged Replace the unit with a new one The sampling nozzle is clogged Replace the sampling nozzle with a new one The MD 640V combination syringe pump Replace the unit with a new one unit failure The UT 7192 AMP CONTROL board Replace the board with a new one failure The UT 7193 POWER board failure Replace the board with a new one The reagent is dirty Replace the reagent with a new one High background The measurement baths and sub baths are Clean or replace the measurement baths and sub noise of HGB dirty baths with new ones The MC 640V measuring unit failure Replace the unit with a new one The UT 7201 HGB AMP board failure Replace the board with a new one The UT 7202 HGB LED board failure Replace the board with a new one The UT 7192 AMP CONTROL board Replace the board with a new one failure The UT 7193 POWER board failure Replace the
55. of the power cord is 0 1 Q Check that the earth leakage current is 0 5 mA or less under normal condition and 3 5 mA or less under each single fault condition 6 5 6 MAINTENANCE Displaying Operation History Screen You can display the total operating time hours total number of counts and number of counts used to determine the maintenance schedule for filters measurement baths sub baths pump tube rinse unit sampling nozzles and cap pierce nozzle Cap pierce nozzle is only necessary for MEK 6400 analyzer HOY 10 05 14 23 When the filters measurement baths sub baths pump tube rinse unit sampling nozzles and cap pierce nozzle are used more than the following number of sample counts the ALARM screen appears to prompt you to check and or replace them Filters measurement baths and sub baths 1 000 counts Check and or replace the above item s on the MAINTENANCE screen Pump tube 3 000 counts Rinse unit 1 000 counts Sampling nozzles 3 000 counts Cap pierce nozzle 1 000 counts 1 Press the OTHER key on the MENU screen to display the OTHER screen HOY 04 05 16 21 am a CALIBRATION SETTINGS DATA OPERATIONS 2 Press the OPER HISTORY key to display the OPER HISTORY screen HOY 04 05 16 40 C PER HISTORY L DAILY OUTPUT CIRCUIT CHEC MAINTENANCE BACKGROUND 6 6 Service Manual MEK 6400 64 10 6420 6 MAINTENANCE The first page displays the software version installed langua
56. one Liquid cannot be Pump tube is damaged Replace the pump tube with a new one drained The MP 640V pump unit failure Replace the unit with a new one The UT 7193 POWER board failure Replace the board with a new one The MC 640V measuring unit failure Replace the unit with a new one Electromagnetic valve failure Replace the UT 7192 AMP CONTROL board with a new one Replace the UT 7193 POWER board with a new one Service Manual MEK 6400 6410 6420 2 17 2 TROUBLESHOOTING A022 WBC level 2 This message appears when the manometer upper sensor detects no liquid but the lower sensor does not detect no liquid at the start of WBC measurement or draining liquid from the manometer Reason Possible Cause Criteria Countermeasure Liquid is drained but liquid cannot be detected LED does not turn off WBC manometer is dirty Perform strong cleaning WBC manometer lower sensor adjustment error Adjust the sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in Section 5 The MC 640V measuring unit failure Replace the unit with a new one Liquid cannot be drained Pump tube is damaged Replace the pump tube with a new one The MP 640V pump unit failure Replace the unit with a new one The UT 7193 POWER board failure Replace the board with a new one The MC 640V measuring unit failure Replace the un
57. performed If the error still occurs replace the battery Reason Possible Cause Criteria Countermeasure The data cannot be The backup data is Replace the CR2450 battery with a new one saved inappropriately changed Replace the UT 7192 AMP CONTROL board with a new one E124 CIRCUIT ERROR Reason Possible Cause Criteria Countermeasure The analyzer cannot count preliminary pulse The MC 640V measuring unit failure Replace the unit with a new one The UT 7192 AMP CONTROL board failure Replace the board with a new one E126 TOUCH PANEL ERROR Reason Possible Cause Criteria Countermeasure Touch panel Cable s on the PV 640V front panel unit is Replace the unit with a new one does not damaged react Connector s on the front panel unit is Reconnect the connector properly disconnected Touch panel failure Replace the PV 640V front panel unit with a new one The UT 7192 AMP CONTROL board failure Replace the board with a new one E141 CAP PIERCE INITIALIZE ERROR The MS 641V cap pierce unit cannot be initialized Reason Possible Cause Criteria Countermeasure Cap pierce does not move Connector s on the MS 641V cap pierce unit is disconnected Reconnect the connector Cable s on the cap pierce unit is damaged Replace the cable with a new one Cap pierce motor failure Replace the MS 641V cap
58. pierce unit with a new one Motor driver on the UT 7193 POWER board failure Replace the board with a new one The sensor cannot detect the starting position of the sample needle Sensor on the cap pierce unit failure Replace the sensor with a new one Cable s on the cap pierce unit is damaged Replace the cable with a new one Service Manual MEK 6400 6410 6420 2 29 2 TROUBLESHOOTING Service Maintenance Screens The various service maintenance screens are provided for checking the operation of a component or block such as pump motor sensor and unit individually and for setting some functions which are usually not changed So these screens are not described on the operator s manual There may be additional function or change of the function which depends on the software version If you found a function which is not shown in this section please contact Nihon Kohden representative before using the function CAUTION Using a key on one of the service maintenance screens without reading can damage the instrument Completely read the corresponding part before using a screen key Displaying the SERVICE Screen 2 30 HOY 04 05 15 26 ID 0001 fa Sample type Dilute mode Sampling mode G Open f Closed GUAE HOY 04 05 16 21 eu CALIBRATION d DATA ex OPERATIONS ETTINGS HOY 04 05 14 40 LANGUAGE 1 Press the
59. the Power On PRIME ON INSTALL i s 9 8 Turning Off the zo E 9 9 Checking ACCUEBOV saz vical eosin Rae icc ede dt save oi rr nun code kostet cl iia 9 10 Checking the Circuit lost EUM 9 10 MEU nmm nnne nen nnhhern enn nnne nnne nnne nnne 9 10 Calibrating i P HM 9 11 Service Manual MEK 6400 6410 6420 9 1 9 INSTALLATION Connecting the Power Cord and Grounding the Analyzer Connecting the Power Cord AC source socket Equipotential Grounding Equipotential ground terminal 9 2 CAUTION Only use the provided power cord Using other power cords may result in electrical shock or other injury to the operator Connect the provided power cord to the AC SOURCE socket on the rear panel and plug the cord into a 3 prong AC outlet If possible use an independent AC outlet only for this instrument The instrument must not share an AC outlet with noise generating equipment such as a centrifuge constant temperature bath thermostat refrigerator air conditioner or ultrasonic cleaner WARNING For operator safety equipotential grounding of all instruments must be performed Consult with a qualified biomedical engineer When more than one electrical instrument is used there may be electrica
60. the counter press the RESET key for lt Sampling nozzle gt on the OPER HISTORY screen to reset the counts to zero Sampling nozzle 9 Fill in the Maintenance Check Sheet Replacing Pump Tube Check the pump tube for water droplets and leaks every day Replace the pump tube when there are water droplets or leaks Once every 4 months or every 3 000 sample counts whichever comes first NOTE Do not leave the pump tube with water droplets or leaks on it Materials Required Powder free gloves lab coat safety glasses Phillips screwdriver Procedure 1 Do the procedure in the Before Maintenance Procedure earlier in this section to turn off the instrument 2 Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then toward you to remove the cover 6 32 Service Manual MEK 6400 6410 6420 6 MAINTENANCE i o El ar S A gle E E a e 8 a Check the pump tube for water droplets and leaks If any droplet or leak is found replace the tube with a new one by using the following steps Pull out the white tube joint from the tube holder and pull out the pump tube by turning
61. the diluent with a new one The diluent bottle is dirty Clean the diluent bottle The fluid path is dirty Press the Clean key on the front panel to perform cleaning Perform strong cleaning Clean the sub baths and measurement baths Clean or replace the filter below the RBC measurement bath with a new one Clean the apertures Replace the measurement baths and sub baths with new ones Replace the aperture with a new one Rinse unit is dirty Clean the rinse unit The sampling nozzle is clogged Replace the sampling nozzle with a new one Noise through AC source Connect the power cord to another AC outlet Put a noise cut filter on the power cord Noise interference Perform equipotential grounding Move the instrument which is producing the noise away from the hematology analyzer The aperture is damaged Replace the aperture with a new one The MC 640V measuring unit failure Replace the unit with a new one Circuit malfunction is performed by pressing MENU key gt OTHER key gt CIRCUIT CHECK key PLT is outside 160 5 when circuit check Replace the UT 7198 MEASURING board with a new one Replace the MC 640V measuring unit with a new one Replace the UT 7192 AMP CONTROL board with a new one Samples are not stirred enough Possible Cause Criteria Countermeasure The sample was not stirred thoroughly before measureme
62. the pump rotator counterclockwise Then pull the black tube joint out of the tube holder Pump rotator 6 Remove the white and black tube joints and replace the pump tube 7 Return the white tube joint to the original position and push the pump tube Tube holder into the tube guide by turning the rotator counterclockwise Pump rotator White Tube guide Pump tube Service Manual MEK 6400 6410 6420 6 33 6 MAINTENANCE Tube holder Pump rotator OPER HISTORY Filters baths amp sub baths 83 1000 834 ma 83 1000 83 3000 Cap pierce nozzle 61 4 1000 A 3338 HOY 04 05 16 40 Pump tube Rinse unit Sampling nozzle 6 34 8 Return the black tube joint to the original position NOTE Be careful not to pinch the new pump tube between the tube guide and housing This may damage the pump tube Do not attach the black tube joint to the tube holder before the white tube joint because internal compressed air may disconnect the tube Put back the pump tube properly If the pump tube has slack remove the slack by turning the rotator clockwise If the pump tube has slack it will be damaged by the tube guide Make sure the joints are held properly by the tube holder as shown below Otherwise the pump tube may be damaged or the life of the pump tube will be shortened Tube holder Tube joint 9 Rea
63. 0 2 15 2 TROUBLESHOOTING Solving Problems from Alarm Messages A001 No diluent This message appears when the sensor in the tank does not detect the diluent during measurement priming or cleaning Reason Possible Cause Criteria Countermeasure No diluent in the tank Out of diluent Replace diluent The tube between the hematology analyzer and diluent is squeezed bent clogged or there is leakage Check the tube connection and if necessary replace the tube with a new one Leakage in the fluid path Check the fluid path If necessary replace tubes Pump tube is damaged Replace the pump tube with a new one The MP 640V pump unit failure Replace the unit with a new one The MD 640V combination syringe pump unit failure Replace the unit with a new one The UT 7192 AMP CONTROL board failure Replace the board with a new one The UT 7193 POWER board failure Replace the board with a new one Air bubbles When the hematology analyzer is left unused the diluent inside the tank may evaporate and enter the fluid path as air bubbles Press the Clean key to perform cleaning press the Reset and Power keys together to turn the power off then press the Power key to turn on Leakage in the fluid path Check the fluid path If necessary replace tubes Failure of the sensor in the tank Air bubbles on the liquid sensor Perform pri
64. 0 CONTENTS Reattaching the Sampler Unit sse 4 22 Removing the Cap Pierce Unit or Rinse Unit sesssssseeeeeneennene 4 23 Reattaching the Cap Pierce Unit or Rinse Unit esssseseeeeee 4 24 Removing the Inlet Outlet Unit xcited oana nnne nnne nnns 4 25 Reattaching the Inlet Outlet Unit essssseseeeeeeeeeenneennen 4 26 Removing the Valve Unit ooooooocccoconanccccccnnnononcnncnnnnnnnnncnnnnnnnnn nn nennen nenne tenen nnne nnns 4 26 Reattaching the Valve Unit oooononncccnnnnnanccccccnnnnonononcnnnonnenonnnnncnnnnnnnnnnnnnnennnnnnnnnns 4 27 Removing the AMP CONTROL Board saisin savindan aaaeaii aaa 4 28 Reattaching the AMP CONTROL Board cooococcnoccccconociccnonnccnonancnnonanonnonanccnanancanannos 4 29 Removing the POWER Board 1 I ROI hee eee eee 4 30 Reattaching the POWER BOArd iconic ion eects 4 31 Removing the MEASURING Board ccoooocccccoocccononcccnonncnnonnnnnnnnnnnonannnnnnnnnnnnnannncenannncaninns 4 32 Reattaching the MEASURING Board sse 4 33 Removing the MIXED PUMP Board seeeeenem enne nnne 4 34 Reattaching the MIXED PUMP Board ssennemn 4 34 Removing the PRINTER DRIVER Board seem 4 35 Reattaching the PRINTER DRIVER Board ssee 4 35 Removing the KEY BOAT iecore erii etae reae etude code etx Yr duae eun nx Adeeb 4 36 Heattaching the KEY Board tectae eo e ed
65. 0 MPV 1 0 In the above example the RBC measured value is 4 43 and the acquired CV is 0 8 0 896 of 4 43 is 0 035 4 43 0 035 is 4 465 and 4 395 Therefore the CV of 0 846 indicates that in 6 of 10 measurements the acquired value is between 4 395 and 4 465 4 37 4 39 4 40 4 43 4 44 4 45 4 45 4 46 4 47 4 48 2 data 6 data 2 data 4 395 4 465 Reference The reproducibility depends on the number of counted cells When this number increases the reproducibility is improved Therefore if the number of counted cells is small a sample with low concentration is measured the variation increases For example the CV is within 4 when counting 300 000 PLT but the CV increases to over 4 when counting 100 000 PLT This is a common feature for any hematology analyzer Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING Checking Accuracy Before checking accuracy measure a MEK 3DN hematology control with the procedure described in the Checking the Reproducibility section Confirm that the obtained sample data is within the acceptable range on the assay sheet attached to the hematology control If the data is outside this range or on the borderline calibrate the hematology analyzer with the following procedure Checking Procedure 1 Measure the MEK 3DN hematology control 10 times on the CALIBRATION screen 2 Calibrate the instrument Refer to Section 7 Calibration in the
66. 0 6410 6420 6 MAINTENANCE Sampling nozzle screw 2 Loosen the sampling nozzle screw from each of the sampling nozzle NOTE Be careful not to drop the screws into the analyzer 3 Turn the sampling nozzle 90 clockwise 4 Remove the joint from each sampling nozzle NOTE Diluent may flow out from the sampling nozzle when the joint is removed 5 Pull the sampling nozzle up to remove it Black joint 6 Insert each new sampling nozzle into the sampling nozzle guide attach the joint and turn the sampling nozzle 90 counterclockwise to lock it into place Make sure that the white joint is attached to the left sampling nozzle front panel side and the black joint to the right sampling nozzle Fasten the sampling nozzles with the sampling nozzle screws Sampling nozzle guide 7 Raise the sampling nozzle plate to the original position Service Manual MEK 6400 6410 6420 6 31 6 MAINTENANCE AAA MOV 04 05 16 40 Filters baths amp sub baths 93 1000 ss 6 Reattach the right side cover and fasten it with the three screws on the rear panel and one screw on the right side panel A 7 Press the Power key to turn on the power The analyzer starts priming the rn 83 7 3000 333 fluid pathway Rinse unit 83 1000 s 93 4 300 3 Cap pierce nozzle OK PREV CE 8 Ifthe sampling nozzles were checked and cleaned the sampling nozzle counter will have to be reset To reset
67. 00 6410 6420 Turning Off the Power Main power lamp CUON gt 8 Power lamp Power key 1 Main power switch Service Manual MEK 6400 6410 6420 9 INSTALLATION To turn the power off press the Power key on the front panel The analyzer automatically performs cleaning and the After cleaning the power will automatically shut off message appears After the cleaning is completed the power is automatically turned off To turn the main power off press the Main power switch on the rear panel Check that the Main power lamp on the front panel is off Always leave the main power on except for storage and transportation of the analyzer 9 9 9 INSTALLATION Checking Accuracy Checking the Circuit Measuring Background Noise 9 10 Check the analyzer before measurement to assure measurement reliability For details on the measurement reliability refer to Section 6 Quality Control of the Operator s Manual Check the analyzer s electrical circuit Refer to Checking the Circuit in Section 6 Count the diluent to measure background noise When using the analyzer in closed mode measure background noise in closed mode When using the analyzer in open mode measure background noise in open mode Closed mode is only for MEK 6400 To measure background noise refer to Measuring Background Noi
68. 1 45V D0112 4 Vp D051 MPSEN D052 MBSEN 7 4 36 V DC terminal for analog circuit 15 V DC terminal for analog circuit Ground terminal for analog circuit 15 V DC terminal for analog circuit Supply voltage Vu terminal for motor control Supply voltage 15 V DC terminal for valves Ground terminal for valves Ground terminal for motor control 5 V DC terminal for digital circuit 3 3 V DC terminal for digital circuit Ground terminal for digital circuit Supply voltage Vcc terminal for secondary power circuit control Supply voltage Vp terminal for optional printer unit Ground terminal for optional printer unit Test point for power key switch status check Power key switch Bit switches for power supply mode selection No 1 ON 5 V power supply always ON No 2 ON ALL power supply always ON LED for supply voltage check for secondary power circuit control When the voltage is supplied to the circuit this LED is lit LED for 15 V DC LED for 36 V DC LED for checking supply voltage for motor control LED for checking supply voltage for valves LED for 45 V DC LED for checking supply voltage for optional printer unit LED for checking position sensor for MP 640V LED for checking sub bath rotation sensor Service Manual MEK 6400 6410 6420 D071 MS1YSEN D072 MSIXSENI D073 MSIXSEN2 D083 MS2HI 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD LED for checking x axis position
69. 10 6420 4 5 4 DISASSEMBLY AND ASSEMBLY Removing the Right Side Cover Top Cover and Rear Cover WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the 3 screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then outward to remove the cover from the chassis 4 Remove the 4 screws which secure the top cover to the chassis 4 6 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Pull the top cover rearward and remove it 6 Loosen the two screws at the bottom of the rear cover Loosen these two screws 7 Remove the 5 screws from the rear cover 8 Remove the rear cover by tilting it rearward and then lifting it up Reattaching the Right Side Cover Top Cover and Rear Cover To reattach the right side cover top cover and rear cover reverse the above procedure Service Manual MEK 6400 6410 6420 4 7 4 DISASSEMBLY AND ASSEMBLY Removing the Front Panel Unit WARNING For your safety before disassembling the hematology analyzer wait
70. 105 LL MCV 85 to 115 fL PLT 152 to 168 10 uL HGB ON 1 5 V to 4 5 V HGB OFF less than 0 5 V If the HGB value is outside the normal range clean the WBC measurement bath and recheck the circuit If a check result is outside the normal range check the cable connection between the MC 640V measuring unit and the AMP CONTROL board Refer to Section 2 Troubleshooting Also check the sensitivity and threshold setting and write down the settings in the maintenance check sheet Press the OK key to return to the OTHER screen Press the OK key to return to the MENU screen Service Manual MEK 6400 6410 6420 Checking the X R Values 6 MAINTENANCE Measure the MEK 3DN hematology control twice on the QUALITY CONTROL screen and check that the X values are within the range on the assay sheet provided with the hematology control and that the R values are within the following range The following range are calculated statistically from the coefficient variation of the instrument WBC 0 7 x 10 uL LY 5 296 MO lt 3 3 GR lt 11 4 RBC 0 29 x 105 uL HGB 1 0 g dL MCV 3 6 fL PLT 39x 10 uL For details on how to measure the hematology control on the QUALITY CONTROL screen refer to Section 6 Quality Control in the Operator s Manual Checking the Current Calibration Coefficients When the X R values are within the range in the previous check you only need to write down the current cal
71. 3 9 2 9 13 8 3 5 138 3 1 HCT 42 1 1 0 42 1 1 0 42 1 0 9 function If the latest 10 measurements include unnecessary data delete the MCV 92 1 0 5 92 2 0 3 92 0 0 5 MCH 30 4 1 5 30 6 1 4 30 5 1 4 unnecessary data beforehand MCHC 33 1 0 6 33 3 1 3 33 1 1 1 PLT 258 1 6 260 1 9 261 1 6 LY 26 8 1 7 27 0 1 2 26 9 1 5 MO 4 0 4 3 3 9 4 3 4 0 4 7 GR 61 3 0 9 61 0 0 6 61 1 0 7 RDW 14 2 2 6 14 1 3 2 14 2 2 7 PCT 0 20 25 2 0 18 21 1 0 19 22 6 MPV 7 0 5 9 6 9 4 5 7 0 5 0 PDW 13 7 2 8 13 8 3 4 13 8 3 1 Total batches 4 Page 21 2 am ae Service Manual MEK 6400 6410 6420 2 39 2 TROUBLESHOOTING INITIAL MENU Screen HOY 04 05 16 21 Cu a OTHER MENU Screen HOY 04 05 16 21 Cu PRINT SETTINGS Screen 2 40 This screen allows you to initialize specified parts or all data in the instrument CALIBRATION Sets all the calibration coefficients to 1000 MEASURED DATA Deletes all the measurement data QC DATA Deletes all the measurement data for quality control and initializes all the settings for quality control SETTINGS Initializes all the settings on the SETTINGS screen This function is same as the INITIALIZE key at the bottom of the SETTINGS screen USER COUNT Resets all the counters for regular replacement parts to zero MAINTENANCE Initializes all the settings on the service maintenance screens FACTORY SET Initializes all the above mentioned settings to factory default settings and del
72. 400 6410 6420 4 21 4 DISASSEMBLY AND ASSEMBLY Reattaching the Sampler Unit To reattach the sampler unit reverse the above procedure NOTE When attaching the sampler unit to the chassis put the sampler unit to the left front side as far as possible Be careful not to pinch the cables of the valve unit Turn the upper coupling clockwise to bring down the sampling nozzle and check that the sampling nozzle on the left front side goes through the opening of the rinse unit Use the lower coupling to adjust the position of the sampling nozzle sideways front and back The cap pierce unit on the MEK 6400 analyzer or rinse unit on the MEK 6410 6420 analyzers position may need to be adjusted Opening of the rinse unit 4 22 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the Cap Pierce Unit or Rinse Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument The MEK 6400 analyzer has the MS 641V cap pierce unit The MEK 6410 6420 analyzers have the MR 640V rinse unit 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove th
73. 410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the PRINTER DRIVER Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the front panel unit Refer to Removing the Front Panel Unit earlier in this section 4 Remove the 4 screws from the PRINTER DRIVER board Reattaching the PRINTER DRIVER Board To reattach the PRINTER DRIVER board reverse the above procedure Service Manual MEK 6400 6410 6420 4 35 4 DISASSEMBLY AND ASSEMBLY Removing the KEY Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the front panel unit Refer to Removing the Front Panel Unit earlier in this section 4 Remove the 5 scre
74. 6400 6410 6420 2 11 2 TROUBLESHOOTING Samples are not stirred enough Possible Cause Criteria Countermeasure The sample was not stirred thoroughly before measurement Stir the sample thoroughly before measurement Hematology analyzer failure Possible Cause Criteria Countermeasure The appropriate amount of hemolyzing Put the HEMOLYNAC3 tube assay into the middle of the bottle reagent is not dispensed Replace the HEMOLYNAC3 tube assay with a new one Replace the MD 640V combination syringe pump unit with a new one Circuit malfunction Replace the MC 640V measuring unit with a new one WBC is outside 8 0 5 when circuit check is performed by pressing MENU key gt Replace the UT 7198 MEASURING board with a new one OTHER key gt CIRCUIT CHECK key Replace the UT 7192 AMP CONTROL board with a new one WBC sample error Replace the MD 640V combination syringe pump unit with a new one In closed mode replace the MS 641V cap pierce unit with a new one In open mode replace the MS 640V sampler unit with a new one Poor HGB Reproducibility The following may be the main reasons for poor HGB reproducibility Measuring unit and fluid path are dirty Reagent is deteriorated Samples are not stirred enough Hematology analyzer failure Measurement bath rinse unit or fluid path is dirty Possible Cause Criteria Countermeasure WBC measurement bath is dirty Press the Cl
75. 6410C Option Service Manual MEK 6400 6410 6420 MD 640V MP 640V PV 641VK JQ 640V JQ 642V MS 640V MR 640V UT 71921 UT 7193 WA 640VK UT 7198 UT 7201 UT 7202 XP 612V XP 602V UT 7200 XP 612V UT 7203 UT 7199 XP 612V XP 612V 1 GENERAL Measuring Unit MEASURING Board HGB AMP Board HGB LED Board 2 Way Electromagnetic Value x 10 2 Way Electromagnetic Value x 2 Combination Syringe Pump Unit MIXED PUMP Board 2 Way Electromagnetic Value x 4 Pump Unit Front Panel Unit KEY Board Inlet Outlet Unit LIQUID SENSOR Board 2 Way Electromagnetic Value x 2 Valve Unit 2 Way Electromagnetic Value x 4 Sampler Unit Rinse Unit AMP CONTROL Board POWER Board Printer Unit PRINTER DRIVER Board 1 GENERAL MEK 6420 Standard Option MEK 6420J MEK 6420K MEK 6420C MD 640V MP 640V PV 641VK JQ 640V JQ 642V MS 640V MR 640V UT 71922 UT 71923 UT 7193 UT 7198 UT 7201 UT 7202 XP 612V XP 602V UT 7200 XP 612V UT 7203 UT 7199 XP 612V XP 612V Measuring Unit MEASURING Board HGB AMP Board HGB LED Board 2 Way Electromagnetic Value x 10 2 Way Electromagnetic Value x 2 Combination Syringe Pump Unit MIXED PUMP Board 2 Way Electromagnetic Value x 4 Pump Unit Front Panel Unit KEY Board Inlet Outlet Unit LIQUID SENSOR Board 2 Way Electromagnetic Value x 2 Valve Unit 2 Way Electrom
76. 641V JQ 642V Valve Unit ooooooccccnccnccocncocnconooncoonconnnonncon nono nnnn nro nono nn nnn 3 7 JQ 641V Valve Unit acia acia 3 7 JQ 642V Valve Unit etuer ia 3 7 MS 640V Sampler Unit 2 22 eintritt aan e c Rex ei 3 8 MS 641V Cap Pierce Unit MEK 6400 only sss 3 8 MR 640V Rinse Unit MEK 6410 6420 only oooonoocccccooccccccoccninnanccnanoncn nono nanonnnn conan cnn nana 3 9 UT 7192 AMP CONTROL BOATO n a a a cnn nann cn nennen enne nnns 3 9 WT7199 POWERBOard C 3 10 UT 7198 MEASURING Board cocccccnocccconoccnnoonccnnonnnnononnncnnnnnnnnnonnn nn nan encarnan nn n ran nn cnannncanannes 3 10 UT 7201 HGB AMP Board and UT 7202 HGB LED Board ccconnocccinocccconccccnonnconinnncanonnos 3 11 UT7200 MIXED PUMP Board 6 etate te roten teat eden eee ee at Ene au 3 11 UT 7205 PRINTER DRIVER Board 2 citt rn tah aci e lia 3 12 UT 7209 dz dizi 3 12 UT 7199 LIQUID SENSOR Board sssssssssseeeeeeeeeene enne eene nnne nnns 3 13 XP 602V XP 612V 2 way Electromagentic Valve ssssssseemene 3 13 Disassembly and Assembly eessesuus 4 1 Before You Begln dp terne a 4 2 Warhings and Cautions iniciar DOE Rut asas 4 2 Required TOS c 4 2 Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the ASTUME Seres ode ie A ia 4 3 Board and Unit Location tt tB t dre obt
77. 9 E141 CAP PIERCEUJNITIAEIZE ERROR iecit etre ra trice pru totes ee nepote bete rats tits 2 29 Service Maintenance Screens sssssssssssssesessseeeeee RRE sent rnr sentes entree 2 30 Displaying the SERVICE Screen sssssssssssssseseeseses eene enne nennen nennen senten aE E ENE EAEE 2 30 CHECK MENU SGEE Emm 2 32 UNIT MAINTE SCHe en LEE 2 32 MD640V ieina hile A adi 2 33 RE GAO E Lc E aeetan ences 2 33 MS 641V MEK A aaa aaa aaa eaa aaa E E ai 2 34 MV PUMP CHK ee 2 34 CONT MEASURE Screen coccccnoccccnnonccnononcnnnonoconnnnnn cnn 2 35 SETTING MENU Screen p ET 2 35 ADV SETTINGS SOM m 2 35 REAGENT FACTOR SGrI66li vasito diia iii 2 36 USER MAINTENANCE Scr66n iiieaiiiiii eda neneda anaran Emedi eiaa kadad ai eaaa kiai Sidana AARE 2 36 PANIC VALUE Screen for MEK 6400 and MEK 6410 0ococcococccccncccccononcnnonancnnonnnconannnco nono canina cnnans 2 37 FLAG SETTINGS Seremi dias 2 37 BAR GODE PORT SCIGGD attt eerta aaiae vane a a aA a e Tu de patte nexus 2 37 UNIF GALL Soren iii o e aa AS 2 38 2 2 Service Manual MEK 6400 6410 6420 MUSNES EEEE EEEE AE EEEE E o 2 38 X10 CGV SGre EM an RM 2 39 INITIALE MENU SFIGOF i euin tte ie 2 40 OTHER MENUS creen tinca rides cte etta dla lei 2 40 PRINT SETTINGS SClee iiss eH MUI Id LM ns 2 40 Service Manual MEK 6400 6410 6420 2 3 2 TROUBLESHOOTING Check Procedure Flowchart Check the hematolog
78. BC aperture manometer The MC 640V measuring unit failure Replace the unit with a new one A010 RBC PRIMING ERROR This message appears when diluent cannot be primed during priming the RBC manometer manometer upper or lower sensor does not detect the diluent Reason Possible Cause Criteria Countermeasure Liquid cannot be RBC manometer upper sensor adjustment error Adjust the sensor Refer to Adjusting the detected even when Upper and Lower Sensor Output Voltages of there is enough liquid RBC manometer lower sensor adjustment error the Manometers in Section 5 LED does not light The MC 640V measuring unit failure Replace the unit with a new one Air bubbles in the RBC aperture is not attached properly Reattach the RBC aperture manometer The MC 640V measuring unit failure Replace the unit with a new one A021 WBC level 1 This message appears when the manometer upper sensor does not detect no liquid at the start of WBC measurement or draining liquid from the manometer Reason Possible Cause Criteria Countermeasure Liquid is drained WBC manometer is dirty Perform strong cleaning but liquid cannot be WBC manometer upper sensor Adjust the sensor Refer to Adjusting the Upper and detected LED does adjustment error Lower Sensor Output Voltages of the Manometers in not turn off Section 5 The MC 640V measuring unit failure Replace the unit with a new
79. Before Maintenance Procedure earlier in this section to turn off the instrument Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed Slide the right side cover to the rear direction and then pull it toward you to remove the cover 4 Remove the 2 filter joint assemblies by turning the tube connectors For MEK 6400 also replace the filter between the MC 640V Measuring Unit and JQ 641V Valve Unit Filter joint Filter 6 22 Service Manual MEK 6400 6410 6420 6 MAINTENANCE 5 Remove the filter from each assembly Use tweezers to remove any dust from the filter If it is still dirty replace it with a new one 6 Reattach the filter joint assemblies to the bottom of the RBC measurement bath and air trap Make sure that the tube with the same number as the Filter packing number label on the attaching part is connected back to the original position Only finger tighten the filter joint Filter packing code no 2114 082062B NOTE When attaching the filter joint assembly be careful not to bend or damage the filter packing at the bottom of the measurement bath If there is leakage noted after installment of the filter check that there are no scratches or damage around the filter Damage may occur if a component is overtightened 7 Reattach the right side cover
80. C Serial Port 1 Serial Port 2 D sub 9 pin IOIOI 1 El IOIOI A El Pin No Signal Pin No Signal Pin No Signal 99 99 FG 4 To pin 6 7 RTS o o 99 9 2 RXD 5 SG 8 CTS le 3 TXD 6 To pin 4 9 NC Printer Socket D sub 25 pin female C SDBB 25S SL LNK 02 E Pin No Signal Pin No Signal Pin No Signal o E 1 XSTROBE 10 XACK 19 SG E S 2 DO 11 BUSY 20 SG o9 3 D1 12 PE 21 SG 4 D2 13 SLCT 22 SG 5 D3 14 NC 23 SG 6 D4 15 XERR 24 SG 7 D5 16 XSLCTIN 25 SG 8 D6 17 SG 9 D7 18 SG USB Socket gt Pin No Signal 1 NC a 2 USB D 3 USB D 4 ED 8 2 Service Manual MEK 6400 6410 6420 Section 9 Installation Connecting the Power Cord and Grounding the Analyzer ooonoccccnnncccconoccncnoncncnonanconana cnn nonrn nn nannn nn nan n cara rra 9 2 Connecting the Power Gord iii e dd e 9 2 Equipotential GFOUNGING ss its 9 2 Connecting Tubes and Installing Reagents sisirin anadan oenina d nn nara nn rennen nennen enn ea aE 9 3 Materials REQUIFSO ccoo aser ESENE ENE ebnenstiys 9 3 Connecting TUDES saires arae ronca nr 9 4 Bien TDG uta ninia oido 9 4 Beam 9 5 Lysing Reagent TUDO c E 9 6 Waste Fluid B V o A A EATARRA 9 6 T fming Power OMOT net 9 7 TUI On thie POWET 2i oorr geet ened NEE ei 9 7 Cleaning the Fluid Path After Turning
81. C DSP POS RBC ASP POS RBC DSP POS Tij 1 CYCLE CONT INITIAL L Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING MD 640V This screen allows you to check the operation of the MD 640V CAUTION When pressing the DISPENSE key place a container under the sampling nozzle because diluent dispenses from the sampling nozzle When using the DISPENSE key or 5 TIMES AUTO key be careful not to let the hemolysing reagent overflow from the WBC measurement bath Always perform INITIALIZE before pressing any other key INITIALIZE Moves the pistons to the initial positions ASPIRATE Moves the pistons to the position for aspiration DISPENSE Moves the pistons to the position for dispense 5 TIMES AUTO Repeats the aspiration and dispense operations 5 times PRIME No function MS 640V This screen allows you to check the operation of the MS 640V CAUTION Before checking the operation check that the sampling nozzle is not bent Check the sub bath positions which should be upside down because it prevents the sampling nozzle from bending Always perform INITIALIZE before pressing any other key INITIALIZE Moves the sampling nozzle to the initial position ASPIRATE POS Moves the sampling nozzle to the position for aspirating a blood sample PIERCE POS Moves the sampling nozzle to the position for the cap pierce lowering motion WBC DSP POS Moves the sampling nozzle to the pos
82. CelitacK Automated Hematology Analyzer MEK 6400 MEK 6410 MEK 6420 MEK 6400C MEK 6410C MEK 6420C MEK 6400J MEK 6410J MEK 6420J MEK 6400K MEK 6410K MEK 6420K If you have any comments or suggestions on this manual please contact us at www nihonkohden com 0634 900086B CONTENTS Contents GENERAL HANDLING PRECAUTIONS sess i WARRANTY POLO Yin tecti eee rien t dedit re te ruit eet fuerint buen tad Ot tinae ii RESPONSIBILITIES PROFESSIONAL USERS seen ii I eus EeHor iore iii Conventions Used in this Manual and Instrument sssssesemn V Warnings Cautions and Notes cccconooocccnnconononnnnnnconononononnconnnononnnncnnnnnn nn rnnccnnannnnnos V Seclon T GODBFal ue rta a IRAE 1 1 INTOdUCIO sex nice 4 E 1 2 sauer m 1 3 Specifications 0 1 4 Measured Parameters Ranges and Reproducibility to Specimen from Venous loe H 1 4 Standardization Analysis Method sssssssseeeene 1 4 Detection Method ococcccccccnnicnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncnnrnninnnnnnnnnnnnnnnnns 1 5 Dilution Ratio sita RER 15 COUNTING TIME c ri 15 BI HER 1 5 seo 1 5 Environmental Conditions oooooccccccnoncoooncnnccnnononnnnnccnnnnnonnnncnnnnnoncnnnccnnnnn nn 1 5 PowerheSequiremlerits eiie DEC nte se cuc Inda tree deserere cien Rr uu hs 1 5
83. E dd AA HOY 04 05 16 40 Filters baths amp sub baths 83 1009 Sampling nozzle D Cap pierce nozzle A Pump tube Rinse unit 17 Reconnect the filter joints to the RBC and WBC measurement bath assemblies by turning the tube connectors 18 Reattach the tube joint to the WBC measurement bath by turning the knurl joint 19 Reattach the HGB cover and fasten it with the screw 20 Reattach the right side cover and fasten it with the three screws on the rear panel and one screw on the right side panel 2 Press the Power key to turn on the power The analyzer starts priming the fluid pathway 22 If the measurement baths and sub baths were cleaned the bath counter will have to be reset When the bath counter is reset the filter counter is also reset Before resetting the counter the filters should be replaced Refer to Replacing Filters earlier in this section To reset the counter press the RESET key for Filters baths amp sub baths on the OPE HISTORY screen to reset the counts to zero 23 Hill in the Maintenance Check Sheet Checking and Cleaning Replacing the Rinse Unit and Cap Pierce Nozzle 6 26 Check and clean the rinse unit and cap pierce nozzle once a month or every 1 000 sample counts whichever comes first WARNING The cap pierce nozzle is sharp and potentially contaminated with infectious materials Be careful when handling the cap pierce nozzle an
84. ERVICE screen must be set to On A030 WBC sample error This message appears when the voltage between the electrodes is out of acceptable range Reason Possible Cause Criteria Countermeasure Diluted sample error The diluent temperature is too low The environment temperature must be from 15 to 30 C for WBC count Specified reagents are not used Only use the specified reagents Service Manual MEK 6400 6410 6420 2 19 2 TROUBLESHOOTING A031 WBC noise 2 This message appears when there is baseline wandering during measurement A032 WBC noise 1 This message appears when the time sequence of counted pulse is not stable Reason Possible Cause Criteria Countermeasure Power line 2 pin power cord is used Use 3 pin power cord Ground lead is not connected Perform equipotential grounding Decrease in the power voltage Do not share the power source with any other instrument Influence Measurement baths and apertures are dirty Clean or replace the measurement baths and apertures from other with new ones malfunction Perform strong cleaning High background noise Refer to Background Noise Problems earlier in this section Poor hemolyzation of the sample Refer to Appears on the right on WBC measured value later in this section Sample aspiration 1s not stable clog Refer to A029 WBC clog alarm Measuring WBC aperture is dirty Perform s
85. EST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD HGB AMP Board E E Mo 14 ES m Jo ofl Ga UNV 89H I SONO 719 Hodi Y N TPO11 EA Ground terminal for analog circuit TP012 HANA Test point for checking HGB sensor output LIQUID SENSOR Board J E P1 6190 900626 JAPAN E HE UT 7199 04 04 A E 61 90 900626 UT 7199 A 5V 5 V DC terminal for digital circuit ED Ground terminal for digital circuit TP014 DIL Test point for liquid sensor output for diluent TP015 LIY Test point for liquid sensor output for hemolysing reagent 7 6 Service Manual MEK 6400 6410 6420 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD MIXED PUMP Board Td S69006 0619 A 003L 1N TOU vo NY dvf TP011 45V TP012 ED D011 ORIGIN D012 ENCODE KEY Board La JAPAN UT 7200 45 04 04 6190 900635 8 POTEP Bl 5 V DC terminal for digital circuit Ground terminal for digital circuit LED for checking bottom position for piston LED for checking encoder output S 6190 9006444 S2 ara a PA 345 g4 MEA D011 MAIN POWER Main power LED lamp Vcc D012 SUB POWER D013 AUTO PRINT Power LED lamp 5 V DC LED for automatic printing mode SWO11 MAIN POWER Power key switch SWOI2 AUTO PRINT Auto print key switch SWO013 FEED SWO014 PRINT SWO015 RESET SW016 CLEAN SWO017 EJECT Service Manual MEK 6400 6410 6420 Feed key switch Print key swi
86. IALIZE SERS E LAE SLE Type of user ii Select Factory user and press the CHANGE key Factory Service Other user uw Other user iii Enter 4321 for the password using the numeric keys and press the Enter key CHANGE ADD USER OK DELETE Service Manual MEK 6400 6410 6420 5 9 5 ADJUSTMENT 2 On the MENU screen press the OPERATIONS key PRIME key to fill the instrument with diluent IN MOV 04 05 16 21 Qa gt DRAN ALL REMOVE CLOG STRONG CLEA ENU ney HOY 04 05 16 21 em ex CALIBRATION SETTINGS d DATA OPERATIONS gt OTHER 3 On the MENU screen press the OTHER key SERVICE key gt MONITOR key gt SENSOR MONITOR key to display the SENSOR MONITOR screen HOY 04 05 16 21 HOY 04 05 16 21 mp Go Cc eum HOY 04 05 16 21 SENSOR HOHITOR OPER HISTORY Wa DAILY OUTPUT CIRCUIT CHEC MAINTENANCE BACKGROUND APOS SENSOR m SERVICE 4 tities ale ite HOY 04 05 16 21 lt Electrode gt LED On LED Off HGB 1 5819 1 719 0 138 1 5804 Temp compensation WBC 26 214 17 8 18 81 RBC 26 614 17 8 18 21 lt Mano Reagent gt Upper Lower WBC manometer 3 924 7 4 334 RBC manometer 4 169 Z 4 254 Diluent 4 434 Lysing reagent 4 614 A T ith reagen d Without reagent gt 3 5 Y Check these 2 values Temperature HGB unit 24 7 TC MC unit 27 8 C Power
87. Influence Measurement baths and apertures are dirty Clean or replace the measurement baths and apertures from other with new ones malfunction Perform strong cleaning High background noise Refer to Background Noise Problems earlier in this section Filter below the RBC measurement bath is Clean or replace the filter with a new one clogged Sample aspiration is not stable clog Refer to A049 RBC clog alarm Measuring RBC aperture is dirty Perform strong cleaning unit failure Clean the RBC aperture RBC aperture is damaged Replace the RBC aperture with a new one The screw securing the measurement bath Tighten the screw is loose The MC 640V measuring unit and chassis Remove the cause are not insulated there may be leakage The MC 640V measuring unit failure Replace the unit with a new one A057 RBC upper manometer dirty The voltage of the upper liquid sensor on the manometer is 2 25 to 2 75 V Reason Possible Cause Criteria Countermeasure Manometer is dirty The RBC manometer is dirty Perform strong cleaning Sensor voltage adjustment error error RBC manometer upper sensor adjustment Adjust the RBC manometer upper sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in Section 5 A058 RBC lower manometer dirty The voltage of the lower liquid sensor on the manometer is 2 25 to 2 75 V Reason Possible Cause Criteria
88. LEANACs3 detergent with CLEANAC detergent appears HOY 09 05 19 24 Strong cleaning complete Replace CLEANAC3 with CLEANAC detergent 5 Replace the CLEANACs3 detergent with CLEANAC detergent and press the OK key The analyzer cleans with the CLEANAC detergent and the screen returns to the READY screen 6 After maintenance is complete and the analyzer is turned on again measure background noise at least twice Draining Measurement Baths and Sub Baths This function drains the measurement and sub baths to avoid spillage of the diluent 1 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen HOY 04 05 16 21 mm emm CALIBRATION SETTINGS DATA OPERATIONS y m Service Manual MEK 6400 6410 6420 6 13 6 MAINTENANCE 2 Press the DRAIN BATHS key on the OPERATIONS screen The Drain baths message appears MM HOY 09 05 18 50 OPERATIONS HOY 09 05 18 56 GOO Drain baths REMOVE CLOG STRONG CLEA E gt ER x O 3 Press the YES key to drain fluids from the measurement baths and sub baths The analyzer starts draining the baths and the Draining message appears on the screen Press the NO key to cancel the procedure After draining the screen returns to the READY screen Turning Power Off CAUTION Turn off the main power before doing maintenance Otherwise the operator may receive electrical shock Press the Power key while ho
89. Morphology and enumeration of human blood platelets J Appl Physiol 3 365 Dec 1950 Brecher G Cronkite EP Service Manual MEK 6400 6410 6420 1 GENERAL Detection Method Blood cell count Electrical resistance detection Hemoglobin Surfactant method colorimetric method Hematocrit Histogram calculation WBC population Histogram calculation Platelet crit Histogram calculation RBC distribution width Histogram calculation Platelet distribution width Histogram calculation Dilution Ratio Venous blood Sample volume 30 uL in normal dilution mode about 50 uL in low dilution mode 10 uL in high dilution mode 5 uL in higher dilution mode low and higher dilution modes are not available on the MEK 6420 analyzer WBC HGB 200 1 in normal dilution mode RBC PLT 40 000 1 in normal dilution mode Pre dilution blood Sample volume 10uL 20 uL WBC HGB 1200 1 600 1 RBC PLT 240 000 1 120 000 1 Counting Time Closed mode about 90 s sample from measurement start to data display MEK 6400 only Open mode about 60 s sample from measurement start to data display Display Display 5 7 inch color LCD with backlight and touch screen keys Resolution 240 x 320 dots Screen size approx 86 x 115 mm Display contents Numerical data histograms measuring conditions alarm message and other messages touch screen keys Data Storage Numerical data for all counted parameters for up to 400 samples and histograms for up to
90. N inlet on the right side panel Remove the detergent tube from the detergent container and put it into the waste container Do not disconnect the waste tube from the WASTE outlet NOTE Make sure that the waste tube is correctly connected Waste comes out from the CLN inlet when DRAIN ALL is performed To handle the diluent detergent hemolysing reagent and waste containers follow the instructions on each package label Waste container 7 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen sus IU MM NOV 09 05 18 50 PRIME DRAINBATHS DRANALL 8 Press the DRAIN ALL key on the OPERATIONS screen A confirmation message appears GS Eran OPERATIONS HOY 09 05 19 25 9 Press YES to start draining During draining the screen shows the 1 Remove CLN tube from detergent Draining message container and insert it into waste container 2 Disconnect ISO3 and HEMO3N tubes from inlets on the hematology analyzer NOTE Never remove tube from WASTE outlet After draining the screen returns to the READY screen Drain all EA NIHON KOHDEN Callar 1 CAUTION Turn off the main power before doing maintenance Otherwise the operator may receive electrical shock Press the Power key while holding down the Reset key The power turns off without cleaning Check that the power lamp is off Service Manual MEK 6400 64
91. NG board 6 Remove the 3 screws which secure the MEASURING board to the measuring unit Screws 7 Cable connectors Spacer bolt HIT 7 Remove the spacer bolt with a hex socket driver and remove the MEASURING board Be careful not to drop the black cap of the manometer Reattaching the MEASURING Board To reattach the MEASURING board reverse the above procedure NOTE Make sure to attach the screw on the MC cover Otherwise it may cause noise Service Manual MEK 6400 6410 6420 4 33 4 DISASSEMBLY AND ASSEMBLY Removing the MIXED PUMP Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Do the procedure in Removing the Combination Syringe Pump Unit earlier in this section to remove the combination syringe pump unit from the chassis 3 Remove the cable connector from the MIXED PUMP board The MIXED PUMP board is located at the left side of the combination syringe pump unit Cable connector 4 Remove the 4 screws which secure the MIXED PUMP board to the combination syringe pump unit and remove the board Reattaching the MIXED PUMP Board 4 34 To reattach the MIXED PUMP board reverse the above procedure Service Manual MEK 6400 6
92. Sensor Output Voltages of the Manometers in not turn off Section 5 The MC 640V measuring unit failure Replace the unit with a new one Liquid cannot be Pump tube is damaged Replace the pump tube with a new one drained The MP 640V pump unit failure Replace the unit with a new one The UT 7193 POWER board failure Replace the board with a new one The MC 640V measuring unit failure Replace the unit with a new one Electromagnetic valve failure Replace the UT 7192 AMP CONTROL board with a new one Replace the UT 7193 POWER board with a new one A043 RBC level 3 This message appears when the manometer lower sensor does not detect the liquid during RBC measurement or aspirating sample Reason Possible Cause Criteria Countermeasure Cannot produce measuring The pump tube is damaged Replace the pump tube with a new one pressure The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure can be RBC aperture is not attached properly Reattach the RBC aperture properly produced but there is leakage The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure can be RBC aperture is clogged Clean or replace the aperture with a new one produced but there is clog The MC 640V measuring unit failure Replace the unit with a new one Service Manual MEK 6400 6410 6420 2 21 2 TROUBLESHOOTING A044 RBC bubble 1
93. UMP board failure Replace the board with a new one The UT 7193 POWER board failure Replace the board with a new one APPEARS ON THE RIGHT OF MCHC MEASURED VALUE may be displayed on the right of MCHC value when the MCHC value is out of the normal range lower than 28 0 or higher than 38 0 g dL MCHC is known to be a stable factor and its physiological variation is approximately 3 independent of the patients Reason Possible Cause Criteria Countermeasure Specific o RBC aggregation Measure with a microscope characteristics of the 7 sample Sample is hemolyzing Measure with a microscope Environmental Specified reagents are not used Only use the specified reagents condition is not E appropriate The reagents are past the expiration date Replace the reagents with the new ones Calibration of the hematology analyzer 1s not correct Calibration of HGB or HCT is not correct Calibrate the hematology analyzer HGB measurement error No hemolysing reagent Put the tube into the bottom of the tank HGB sensor voltage adjustment error Adjust the HGB sensor voltage Refer to Adjusting the HGB Sensor Output Voltage in Section 5 HCT measurement error High background noise Refer to the Background Noise Problems earlier in this section Dilution ratio error The MD 640V combination syringe pump unit failure Replace the unit with a
94. WBC differential parameters are derived from the WBC count therefore the limitations for WBC also affect these parameters LY and LY Erythroblasts certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count MO and MO c Large lymphocytes atypical lymphocytes blasts and excessive number of basophils may interfere with an accurate MO count GRand GR Excessive eosinophils metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate GR count and GR EO Abnormal granules may interfere with an accurate EO count Service Manual MEK 6400 6410 6420 1 15 Section 2 Troubleshooting y Check Procedure Flowchart ococcnoccconoccccnonoccccnnoncnnnnrn cn cnn cnn 2 4 Operating Condition and Blood Sample Checks oooocccinnocccnnoccccononcncnnnoncnconnnnncnnn cnc nano cnn cnn cnn ran rr nennen entries 25 Operating Condition CHECK i iori eoa ie crt o censet bei e ege naa a aa esada s ua ANE e a dels 2 5 Blood Sample Handling Check sissisota asnan aai ie nennen seen nnns entren nennt s nennen 2 5 Pre dilution Sample Preparation Check sessssssssesssseseeeeeeennen nennen nennen enne nnns entrent 2 5 Checking the Hematology Analyzer oooccccnnoccccnccccccnnncccnnnoncnnono cnn cnn cnn narrar 2 7 Checking Background NOISE rosca il rial nte 2 7 Measures are 2 7 Parameter Data Check with DilUeNt ooooonccccnnnccccnoocccnonocanonnncncnnnnnonnnnn non enne n
95. a check mark is placed in the check box and the valve is open When pressing the check box with a check mark the check box is cleared and the valve is closed There are five keys for the MP 640V pump unit The function of each key is shown below lt lt High speed clockwise rotation lt Low speed clockwise rotation STOP Stops the rotation gt Low speed counterclockwise rotation gt gt High speed counterclockwise rotation When pressing the MONITOR key at the bottom of the screen the SENSOR MONITOR screen which shows each sensor status appears Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING CONT MEASURE Screen HOY 04 05 16 21 This screen allows you to check the instrument during continuous measurement NOTE Before starting the continuous measurement check that each reagent volume and waste container capacity are enough to do the continuous measurement 10 TIMES Continuously measures the sample 10 times 20 TIMES Continuously measures the sample 20 times 30 TIMES Continuously measures the sample 30 times EVER Measures the sample until you press the Reset key on the front OK panel SETTING MENU Screen HOY 04 05 16 21 The screen has settings which are not usually changed ADV SETTINGS Advanced settings REAGENT FACTOR REAGENTFACTOR Reagent consumption ratio setting ASER MAINTE o USER MAINTE Measurement times settings for user maintenance ANIC VALUE PANIC VALUE
96. a proper grounding facility is available 3 Before Operation a 2 Check that the instrument is grounded properly G 4 Pay extra attention when the instrument is in combination with other instruments to avoid misdiagnosis or other Check that the instrument is in perfect operating order Check that all cords are connected properly problems 5 6 Check that battery level is acceptable and battery condition is good when using battery operated models All circuitry used for direct patient connection must be doubly checked 4 During Operation 1 Both the instrument and the patient must receive continual careful attention 2 Turn power off or remove electrodes and or transducers when necessary to assure the patient s safety 3 Avoid direct contact between the instrument housing and the patient 5 To Shutdown After Use 1 Turn power off with all controls returned to their original positions 2 Remove the cords gently do not use force to remove them 3 Clean the instrument together with all accessories for their next use 6 The instrument must receive expert professional attention for maintenance and repairs When the instrument is not functioning properly it should be clearly marked to avoid operation while it is out of order 7 The instrument must not be altered or modified in any way 8 Maintenance and Inspection 1 The instrument and parts must undergo regular maintenance inspection at
97. ages of the Manometers in diluent LED does WBC manometer lower sensor Section 5 not light adjustment error The MC 640V measuring unit failure Replace the unit with a new one Air bubbles in the WBC manometer is dirty Perform strong cleaning manometer WBC aperture is not attached properly Reattach the WBC aperture with a new one The MC 640V measuring unit failure Replace the unit with a new one A025 WBC bubble 2 This message appears when the manometer upper sensor detects the liquid during WBC measurement or draining manometer from upper sensor OFF to lower sensor OFF Reason Possible Cause Criteria Countermeasure Air bubbles in the WBC manometer is dirty Perform strong cleaning manometer The MC 640V measuring unit failure Replace the unit with a new one 2 18 Service Manual MEK 6400 6410 6420 A026 WBC bubble 3 2 TROUBLESHOOTING This message appears when the manometer lower sensor detects no liquid during WBC measurement or aspirating sample from lower sensor ON to upper sensor ON Reason Possible Cause Criteria Countermeasure Air bubbles in the WBC manometer is dirty Perform strong cleaning manometer WBC aperture is not attached properly Reattach the WBC aperture The MC 640V measuring unit failure Replace the unit with a new one A027 WBC bubble 4 This message appears when the WBC measurement or aspirating sa
98. agnetic Value x 4 Sampler Unit Rinse Unit AMP CONTROL Board MEK 6420J K AMP CONTROL Board MEK 6420C POWER Board Printer Unit PRINTER DRIVER Board Service Manual MEK 6400 6410 6420 1 GENERAL Interference Substances WBC HGB High WBC When WBC count is outside the measurable range measure the sample in high dilution mode If the WBC count is still outside the range dilute the sample further Nucleated erythrocyte Nucleated erythrocyte is detected as WBC and causes increase in WBC count Unlysed red cells In some rare occasions the RBC in the blood sample may not completely lyse and these non lysed RBC may be detected as WBC and cause increase in WBC count Multiple myeloma The precipitation of proteins in multiple myeloma patients may increase the WBC count Leukemia WBC is fragile in leukemia patients and WBC may be destroyed during measurement These WBC fragments may also interfere with WBC differential measurement Chemotherapy Cytotoxic and immunosuppressive drugs cause low WBC count Cryoglobulins Cryoglobulin may be increased in patients who are pregnant or have myeloma cancer leukemia macroglobulinemia lymphoproliferative disorders metastatic tumors autoimmune disorders infections aneurysm thromboembolic phenomena diabetes etc which cause increase in WBC RBC or PLT counts and HGB concentration In such cases warm the blood sample to 37 C in a water bath for 30 minutes and meas
99. ajor components in the instrument Only qualified service personnel should remove replace and install major components WARNING e To avoid the possibility of injury to yourself or damage to the instrument do not install or remove any component or change switch settings while the power is on and wait 10 minutes before installing to or removing any component from the instrument after the power is off To avoid accidental discharge of static electricity which could damage the instrument components use a wrist ground strap when installing or removing any component of the instrument When replacing any parts or units in the instrument do not touch any part of the instrument where blood is or may have contacted Wear rubber gloves to prevent infection by blood CAUTION Before connecting or disconnecting any cables turn off the instrument and unplug the AC power cord from the instrument Fuses cut off the power when an abnormality occurs in the instrument Eliminate the malfunction before replacing the fuse Use the correct fuse only The fuse rating is shown on the holder Removal and replacement of any component in the instrument should be done by qualified service personnel Only use parts recommended by Nihon Kohden to assure maximum performance from your instrument Anti static bench mat Wrist ground strap Phillips screwdriver insulated type Flat blade screwdriver insulated type He
100. alues and examination of the stained blood film Very large PLT Large PLT may exceed the PLT threshold and might not be counted which results in low PLT count Chemotherapy Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count In such a case measure manually Hemolysis Hemolyzed specimens contain red cell stroma which may increase PLT count Anticoagulated blood Blood anticoagulated with acid citrate dextrose may have clumped PLT which may cause decrease in PLT count Agglutinated PLT Clumped PLT may decrease PLT count and or increase WBC count For such sample collect the sample in sodium citrate anticoagulant and measure only PLT The PLT result must be corrected for the sodium citrate dilution effect Very large PLT Large PLT may exceed the PLT threshold and not be counted which results in low MPV Very small fragments Very small RBC RBC fragments and WBC fragments may interfere with MPV measurement Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in erroneous MPV This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Service Manual MEK 6400 6410 6420 1 GENERAL Chemotherapy Cytotoxic and immunosuppressive drugs may affect MPV In such a case measure manually NOTE Blood samples collected in EDTA do not maintain stable MPV because platelets swell depending on the interval after collection and storage temperature
101. aning the analyzer Otherwise the operator may receive electrical shock Service Manual MEK 6400 6410 6420 6 15 6 MAINTENANCE Disinfecting the Surface of the Analyzer NOTE Disinfect the sampling nozzle before every maintenance because infectious blood may be adhered to it Wipe the analyzer with a cloth moistened with disinfecting ethanol Disinfect the sampling nozzle open mode thoroughly because it touches blood Cleaning the Surface of the Analyzer CAUTION Never use organic solvents such as thinner or acetone because they damage the enclosure of the analyzer NOTE When wiping the analyzer with a cloth moistened with water wring out the cloth to prevent water from entering the analyzer 1 Dilute a neutral detergent with water 2 Wipe off the dirt with a soft cloth moistened with the diluted neutral detergent 3 Wipe the analyzer with a dry soft cloth Checking the Reagents Connection Tubes Check that the reagent tubes are not damaged bent or clogged For correct tube connection refer to Connecting Tubes and Installing Reagents in Section 9 Checking the Power Cord and Grounding Lead Check that the power cord and grounding lead are properly connected and not damaged Checking the External Instrument Connection When using an optional printer PC and hand held bar code reader check that they are properly connected to the analyzer Check that the connection cables are not
102. are within the range on the assay sheet 9 11
103. assembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Do the procedure in the Accessing the Connectors on the POWER Board earlier in this section to open the POWER board 3 Remove all cable connectors from the POWER board so that the board can be opened fully 4 Remove the 2 screws which secure the AC inlet to the chassis and pull out the AC inlet rearward so that the screws and connectors of the transformer can be easily accessed 5 Remove the 3 screws which secure the transformer to the chassis Remove the screw which secure the earth lead to the terminal Remove the 3 cable connectors Remove the transformer from the chassis Screws Reattaching the Transformer To reattach the transformer reverse the above procedure 4 40 Service Manual MEK 6400 6410 6420 Section 5 Adjustment General oia act oido playa liinda iii 5 2 Adjusting the HGB Sensor Output Voltage coconcccccnnccccnnoncccconnncnnonnncanonencnnnnnn cnn nan n narran cnn 5 3 Adjusting the Upper and Lower Sensor Output Voltages of the Manometers cccccnooocccccccnnconccnncccnnnnononccnnnnnnnnos 5 6 Adjusting the Liquid Sensor Output Voltages cooococcnnoccccnoncccnonancncnonnnononenccnono cnn non cnn non di nr n rancia nnns 5 9
104. ay be cause for voiding this warranty Defective products or parts must be returned to NKC or its authorized agents along with an explanation of the failure Shipping costs must be pre paid This warranty does not apply to products that have been modified disassembled reinstalled or repaired without Nihon Kohden approval or which have been subjected to neglect or accident damage due to accident fire lightning vandalism water or other casualty improper installation or application or on which the original identification marks have been removed In the USA and Canada other warranty policies may apply RESPONSIBILITIES PROFESSIONAL USERS This instrument must be used by a professional user with a full knowledge of operating this instrument only for his her intended use and according to the instructions for use Instructions in the operator s manual must be followed especially the following points e Storage and stability of reagents e Handling of reagents e Instrument installation e Connection of all tubes to inlets and outlets e Connection of all tubes to reagents and waste container e Checking the amount of reagents and waste fluid e Calibration e Quality control e Maintaining and servicing If deviating from the instructions the professional user does it at the risk and liability of the laboratory and only after validation by the laboratory Nihon Kohden has no responsibility over such deviations ii Servic
105. board 24 9 C OK AWPUT CAL 5 10 Service Manual MEK 6400 6410 6420 5 ADJUSTMENT 4 Check the values on the screen With diluent in the instrument both values should be less than 1 5 V If not press the INPUT CAL key at lower part of the screen to change the correction factors 1 Touch the setting value or use the arrow keys to move the cursor to the setting value you want to change Cursor Move cursor 180 Correction factors Diluent 5535 Lysing 5535 Diluent Diluent and detergents Lysing Hemolysing reagent Numeric keys ii Enter the value using the numeric keys and press the Enter key When the value is increased the voltage also increases When the value is decreased the voltage also decreases iii Press the OK key to return to the SENSOR MONITOR screen and see if both values are less than 1 5 V iv When both values are less than 1 5 V go to the next step If not repeat steps 1 to 111 until both values are less than 1 5 V 5 On the MENU screen press the OPERATIONS key DRAIN ALL key to drain the fluid from the instrument Follow the instructions on the screen Make sure that the tubes are correctly connected 6 Repeat steps 3 and 4 so that both values become above 3 5 V 7 When the liquid sensor output voltages are correctly adjusted return the type of user to the previous type Service Manual MEK 6400 6410 6420 5 11 Section 6 Maintenance General
106. cking that the cables are not caught Completely separate the front panel unit from the chassis Reattaching the Front Panel Unit To reattach the front panel unit reverse the above procedure Service Manual MEK 6400 6410 6420 4 9 4 DISASSEMBLY AND ASSEMBLY Installing the WA 640VK Printer Unit Option WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the front panel unit Refer to Removing the Front Panel Unit earlier in this section 4 Remove the 8 screws which secure the blank panel to the front panel unit and remove the blank panel Blank panel 5 Remove the 2 screws which secure the printer blank panel to the backside of the front cover 6 Remove the printer blank panel from the front side of the front panel unit while depressing the stopper downward and pulling the printer blank panel as shown left Stopper Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 7 Attach the optional WA 640VK printer unit without printer door to the front cover with 8 screws During this step chec
107. d 9 9 Checking ACCUIaCy PE nn nn ran Naaa Teana n ENADE AEE ANa En Ahaaa Sha caita 9 10 Checking the Circuit aru 9 10 Measuring Background NOISO sissies daniodd 9 10 Calibrating Lernen ederet rar rte ci 9 11 Service Manual MEK 6400 6410 6420 EY GENERAL HANDLING PRECAUTIONS This device is intended for use only by qualified medical personnel Use only Nihon Kohden approved products with this device Use of non approved products or in a non approved manner may affect the performance specifications of the device This includes but is not limited to batteries recording paper pens extension cables electrode leads input boxes and AC power Please read these precautions thoroughly before attempting to operate the instrument 1 Tosafely and effectively use the instrument its operation must be fully understood 2 When installing or storing the instrument take the following precautions 1 Avoid moisture or contact with water extreme atmospheric pressure excessive humidity and temperatures poorly ventilated areas and dust saline or sulphuric air 2 Place the instrument on an even level floor Avoid vibration and mechanical shock even during transport 3 Avoid placing in an area where chemicals are stored or where there is danger of gas leakage 4 The power line source to be applied to the instrument must correspond in frequency and voltage to product specifications and have sufficient current capacity 5 Choose a room where
108. d noise The result is displayed after the measurement is complete Parameter Data Check with Diluent Check that the background check values are less than or equal to the following values Disregard the other parameter values because noise does not affect the other parameters Especially check the data for the PLT parameter because PLT is more affected by dust particles than the other parameters When there are dust particles smaller than the WBC and RBC parameters in the diluent the WBC and RBC are not affected by dust but the PLT value increases because the platelets are about the same size as the dust particles If PLT is over 10 x 10 uL do the action described below to reduce the background noise Best values Acceptable values Values in the operator s manual WBC x 107 UL 0 0 0 1 0 2 RBC x 10 uL 0 00 0 01 0 05 HGB g dL 0 0 0 1 0 1 PLT x 107 4L 3 9 10 Service Manual MEK 6400 6410 6420 2 7 2 TROUBLESHOOTING 2 8 Reducing Background Noise To reduce the background noise when the background check value is outside the optimal range shown in the previous tables do the following 1 Press the Clean key on the front panel to perform cleaning If this does not reduce the background noise do the following steps 2 Thoroughly clean the diluent container with detergent 3 Wash away any remaining detergent in the hydraulic system with clean diluent 4 Perform the background check to make sure tha
109. d other forms of recovery of such wastes so as to reduce the disposal of waste Contact your Nihon Kohden representative for disposal at the end of its working life iv Service Manual MEK 6400 6410 6420 Conventions Used in this Manual and Instrument Warnings Cautions and Notes Warnings cautions and notes are used in this manual to alert or signal the reader to specific information WARNING A warning alerts the user to the possible injury or death associated with the use or misuse of the instrument CAUTION A caution alerts the user to possible injury or problems with the instrument associated with its use or misuse such as instrument malfunction instrument failure damage to the instrument or damage to other property NOTE A note provides specific information in the form of recommendations prerequirements alternative methods or supplemental information Service Manual MEK 6400 6410 6420 lt vi This page is intentionally left blank Service Manual MEK 6400 64 10 6420 Section 1 General INTOdUCION Pe eee 1 2 sre 1 3 Specific NS iii P M 1 4 Measured Parameters Ranges and Reproducibility to Specimen from Venous Blood 1 4 Standardization Analysis Method ooooonocccnnnccccnnnocccononcccnnnnncn nono nnn nano nc nnnn cnn nan n cnn nennen nnnm nennen nennen 1 4 Detection Method iiec ent ai lille 1 5 DILO MRAIO m PEE 1 5 Counting TIME III 1 5 BIA
110. d performing this procedure Cap pierce nozzle is only on the MEK 6400 analyzer Materials Required Powder free gloves lab coat safety glasses Phillips and flat blade screwdrivers Cotton swabs e CLEANAC 3 detergent Lint free pad New cap pierce nozzle when required code no YZ 0342 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Procedure 1 Do the procedure in the Before Maintenance Procedure earlier in this section to turn off the instrument 2 Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then toward you to remove the cover 4 Slide the sampling nozzle plate to the right Sampling nozzle plate 18 H Ue tr Cap pierce nozzle top view a Rinse unit top view 5 Check the parts indicated in the illustration for dirt or blood clot Remove blood or salt Rinse unit bottom view seen from the front panel side crystals on the rinse unit and the tip of the cap pierce nozzle with a cotton swab or lint free pad moistened w
111. d starts counting Printer unit 15 WA 640VK Thermal array printer Prints out measured data and sample ID no optional 16 Printer door i us e paper of the WA 640VK printer unit To open pull the upper left corner Service Manual MEK 6400 6410 6420 Ta 1 GENERAL Right Side Panel 1 8 1 2 3 4 e En du E LJ AAN A CEIT No Name Description ISO3 1 f 1 Inlet for the ISOTONAC 3 diluent Diluent inlet CLN 2 Detesient iniet Inlet for the CLEANAC detergent HEMO3N 3 Bemolysiigutagent iiei Inlet for the Hemolynace3N hemolysing reagent 4 WASTE Outlet for waste such as used lyse detergent and aspirated Waste outlet samples Service Manual MEK 6400 6410 6420 Rear Panel 1 GENERAL No Name Description 3 Supplies the power to the analyzer when it is turned on Under l Ma Ponen normal conditions keep this switch turned on 2 Fuse holder Contains the time lag fuse To replace the fuse contact your Nihon Kohden representative AC SOURCE 3 AC source socket Connects the AC power cord to supply AC power to the analyzer 4 Equipotential ground Connects the ground lead to the equipotential ground terminal on terminal the wall for earth grounding Connects to the optional hand held bar code reader 5 ZK 820V Supplies the power to the bar code reader when connected Bar
112. damaged 6 16 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Checking the Power On When the analyzer is turned on check that the self check is performed and the READY screen appears Check that no alarms appear and the touch screen keys function properly Also check that there is no smell heat or noise and that there is no leakage from the analyzer FA NIHON KOHDEN Calibrating the Touch Screen Calibrate the touch screen when the pressed position and operating position do not match Press the Eject key MEK 6400 or Count key MEK 6410 6420 while holding down the Reset key The TOUCH SCREEN CALIBRATION Screen appears 2 Follow the instructions on the screen to calibrate the screen NOTE Do not use a sharp object to press the mark Use your finger After calibration is completed the screen returns to the READY screen Checking the Date and Time Check that the date and time on the screen are correct To change the date and time refer to Setting Date and Time in Section 3 of the Operator s Manual Date Time HOY 04 05 15 26 ID 0001 GEN Dilute mode 3 Normal Service Manual MEK 6400 6410 6420 6 17 6 MAINTENANCE Clock Accuracy At an operating temperature of 25 C the accuracy of the clock IC of the analyzer is about 52 seconds per month At storage temperatures between 20 and 60 C the accuracy of the clock IC of the anal
113. e high Measurement is performed at temperatures above 30 C Room temperature low Measurement is performed at temperatures below 15 C Perform measurement at temperatures between 15 to 30 C The analyzer cannot measure the temperature Sensor failure Replace the UT 7202 HGB LED board with a new one The UT 7192 AMP CONTROL board failure Replace the board with a new one A093 Internal temperature high A094 Internal temperature low A095 Power supply temp high This message appears when the internal temperature is abnormal Reason Possible Cause Criteria Countermeasure Internal temperature is abnormal Actuator moves inappropriately Circuit error Temperature sensor failure Replace the electromagnetic valve motor unit or board which is causing the abnormal temperature Service Manual MEK 6400 6410 6420 2 25 2 TROUBLESHOOTING APPEARS ON THE RIGHT OF WBC MEASURED VALUE may be displayed on the right of WBC value when the RBC ghosts affect the WBC measurement During counting the hematology analyzer detects the RBC pulses which are under the threshold for WBC Reason Possible Cause Criteria Countermeasure Specific High RBC membrane resistance and many Dilute the sample in the pre dilution mode and characteristics of RBC ghosts measure it in pre dilution mode or measure the the sample stained sample with a microscope
114. e Aa 2 18 A025 WBC DUbDlS 2 nicotina ii ica 2 18 A026 WBC bubble 3 NN 2 19 A027 WBC bu bble 4 2 IL ds ida 2 19 A029 WBC ClOG C 2 19 A030 WBC Sample ertOr iioi n pepe tune ada 2 19 A031 WBC DOIS 2 io e p io 2 20 A032 WBC NOISE p 2 20 A036 WBC upper manometer dirty ssis eisini nasii eaa inaa aiaa 2 20 A037 WBC lower manometer dirty cacaos ed e 2 20 AVAT RBG level T merria eaa oea a ML 2 21 A042 RBC level Zinc ae oaio a a D AEREA N NE AE EES 2 21 A0493 RBC level cq 2 21 A044 RBC DUDDIG 1 iiim diee ri ater oa ea bor rette EEA RE iiie ES 2 22 A045 ABC bubble 2 ne ni 2 22 A046 RBC bubble B ooooconiccccccoccccccocnccconncnncnonccononancncnnnc nn enne nne nent nnn denn rnns rra nera ran rca nnne 2 22 Service Manual MEK 6400 6410 6420 2 1 A047 RBC bubble 4 ccccccccccccccccnocccccnnonoconononancnnnnnnnccnnonnnancnnnnnnnnranononancnnnnnnnnnnnonnnancnnnnnnancano ninas 2 22 A049 RBO ClOG mint 2 22 AQ5O RBG Sample Ort EE 2 23 Meng 2 23 A052 PLT NOSO T iii 2 23 A053 PLT NOSO Sii ad PEE AAE AREORA ES AEE ATEAREN EEEE a RESA 2 23 A057 RBC upper manometer dirty ooocoooccccnnoccinonaccnoncinnnnncnonna cnn nan nnn naar nn cnn nn niasa cnn nennen nnne nnns 2 23 A058 RBC lower manometer dirty 2 rete dere tds 2 23 A061 HGB voltage low sssesssssssseseeneeeeneneen nennen nnn aana net aid Eais nnne
115. e JQ 640V inlet outlet unit do not detect the hemolysing reagent during measurement priming or cleaning Reason Possible Cause Criteria Countermeasure No hemolyzing The tube between the hematology analyzer Check the tube connection and if necessary replace reagent flow and HEMOLYNAC3 hemolysing reagent is the tube with a new one squeezed bent clogged or there is leakage The MD 640V combination syringe pump Replace the unit with a new one unit failure Failure of the Air bubbles on the liquid sensor Perform priming sensor Voltage adjustment error of the liquid sensor Adjust the voltage Refer to Adjusting the Liquid Sensor Output Voltages in Section 5 Liquid sensor failure Replace the UT 7199 LIQUID SENSOR Board with a new one A009 WBC priming error This message appears when diluent cannot be primed during priming the WBC manometer manometer upper or lower sensor does not detect the diluent Reason Possible Cause Criteria Countermeasure Liquid cannot be WBC manometer upper sensor adjustment error Adjust the sensor Refer to Adjusting the detected even when Upper and Lower Sensor Output Voltages of there is enough liquid WBC manometer lower sensor adjustment error the Manometers in Section 5 LED does not light The MC 640V measuring unit failure Replace the unit with a new one Air bubbles in the WBC aperture is not attached properly Reattach the W
116. e Manual MEK 6400 6410 6420 EMC RELATED CAUTION This equipment and or system complies with the International Standard EN61326 1 for electromagnetic compatibility for electrical equipment and or system for measurement control and laboratory use However an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326 1 can cause harmful interference to the equipment and or system or cause the equipment and or system to fail to perform its intended function or degrade its intended performance Therefore during the operation of the equipment and or system if there is any undesired deviation from its intended operational performance you must avoid identify and resolve the adverse electromagnetic effect before continuing to use the equipment and or system The following describes some common interference sources and remedial actions 1 Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or cellular phone Install the equipment and or system at another location if it is interfered with by an emitter source such as an authorized radio station Keep the emitter source such as cellular phone away from the equipment and or system 2 Radio frequency interference from other equipment through the AC power supply of the equipment and or system Identify the cause of this interference and if possible remove this interference source If this is not possible use a different po
117. e cable connectors from the POWER board MEK 6400 For the MS 641V cap pierce unit remove red and blue cable connector large and blue cable connector small MEK 6410 6420 For the MR 640V rinse unit remove the blue cable connector LIQUID SENSOR board POWER board Large connector for MS 641V AMP CONTROL board Small connector for MS 641V and MR 640V Also remove cable binder from the cable To access the POWER board refer to Accessing the Connectors on the POWER Board earlier in this section Service Manual MEK 6400 6410 6420 4 23 4 DISASSEMBLY AND ASSEMBLY 4 Remove the front panel unit Refer to Removing the Front Panel Unit earlier in this section 5 MEK 6400 Remove the 2 tube joints from the cap pierce unit and 2 tube joints from the rinse unit MEK 6410 6420 Remove the 2 tube joints from the rinse unit These 2 tube joints are y only for MEK 6400 6 Remove the 4 screws which fasten the cap pierce unit rinse unit to the chassis 7 Pull out the cap pierce unit rinse unit toward you Reattaching the Cap Pierce Unit or Rinse Unit To reattach the cap pierce unit rinse unit reverse the above procedure NOTE After reattaching the cap pierce unit rinse unit check that the sampling nozzle on the left front side goes through the opening of the rinse unit On the sampler unit turn the upper coupling clockwise to br
118. e procedures and provides the maintenance check sheet for documenting the activity NOTE When performing any maintenance procedure perform strong cleaning and drain baths prior to turning the power off and document the activity After maintenance and turning the power on measure background noise run without a specimen until results are within specifications followed by running hematology control Refer to Measuring Background Noise in Section 2 of the Operator s Manual The maintenance schedule outlined on the following page will minimize operational problems with the analyzer The recommended intervals are based on analyzers operating in laboratories that process samples from a general patient population These intervals are affected by several factors including the following Number of samples processed Work load schedule Operating environment Patient population being analyzed Each laboratory must assess its own situation and modify these recommended intervals as necessary WARNING Protect yourself from infection before cleaning and doing maintenance Be careful not to directly touch any place where blood is or may have contacted CAUTION Gloves should be worn during the maintenance procedures They should be powder free as powder may cause instrument problems Service Manual MEK 6400 6410 6420 6 3 6 MAINTENANCE Disposing of Waste NOTE Overdue maintenance is usual
119. ean key on the front panel to perform cleaning Perform strong cleaning Clean the WBC measurement bath Replace the WBC sub bath with a new one Replace the WBC measurement bath with a new one Drain path of the WBC measurement bath is Replace the hemoglobin filter below air trap with a new one dirty Clean the air trap with a cotton swab Rinse unit is dirty Clean the rinse unit Deterioration of reagents Possible Cause Criteria Countermeasure The specified reagents are not used Use only the specified reagents Deterioration of reagents Replace the reagents with new one Use the reagents in appropriate conditions The reagents are used outside the specified temperature range Use the reagents in the appropriate temperature Samples are not stirred enough Possible Cause Criteria Countermeasure The sample was not stirred thoroughly before measurement Stir the sample thoroughly before measurement 2 12 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING Hematology analyzer failure Possible Cause Criteria Countermeasure The appropriate amount of hemolyzing Put the HEMOLYNAC3 tube assay into the middle of the bottle reagent is not dispensed Replace the HEMOLYNAC3 tube assay with a new one Replace the MD 640V combination syringe pump unit with a new one HGB sample error Replace the MD 640V combination syringe pump unit with a new one
120. eeeeeeeseeanes 2 9 Checking the Reproducibility eeeseeseeeseeeeeeeeeeennnenennneee enne 2 9 Checking Procedure cuco nied og ha ed 2 9 ACUTE 2 10 eiae eopiteuirin c EP 2 11 GheckingProcedure Sene 2 11 Poor WBC Reproducibility eeessesesseseee eene 2 11 Poor HGB Reproducibility inn 2 12 Poor RBG Reproducibility sis 12 rero terree erepta rg rta pc partu 2 13 Poor PET Reproducibility zs safe eie titi 2 14 Poor HOT or MCV Reproducibility oooonococnnnnicccnnocccconcccnnoncacc asana 2 15 Solving Problems from Alarm Messages sssssssseeeeeneeeenenenn nennen 2 16 AOOT SING lot emu EE 2 16 A005 No detergent eL S 2 16 A007 No lysing reagent sessseeeenmenn menn 2 17 A009 WBC priming SITO suit cada o te 2 17 A010 RBC PRIMING ERROR 25 et tret eb edo drea cds dus 2 17 A021 WBC level llas aid rrr tona iom nest puma ede a guns eden 2 17 A022 WBC level 2 liie esu pe Ae ee 2 18 A023 WBC level 3 inncocinnnccconnccccnncccccononnnnnnnn conan ncn non rc rra entren nnne nnns 2 18 A024 WBO b bble 1 5 erit rint reete adas 2 18 A025 WBO bubble 2 ett iia 2 18 NANA 2 19 A027 WBC bubble 4 uan Ree ed Ae ees 2 19 A029 WBC ClOG sist tias 2 19 A030 WBC sample error sss menn 2 19 A031 WBC NOISE 2 icon id ds 2 20 A032 WBC noise 1 sssssssssssesseseseeeeeennenenn nennen nnne nnns nennen 2 20 A036 WBC u
121. emove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the cable connector from the POWER board red cable Remove the cable binder from the cable To access the POWER board refer to Accessing the Connectors on the POWER Board earlier in this section Cable binder transparent Red cable Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Move the sampling nozzle plate to the position shown below in ox Sampling nozzle plate i Rotate the upper coupling counterclockwise when looked from the front view to raise the sampling nozzle plate up Upper coupling ii Rotate the lower coupling counterclockwise when viewed from the front to move the sampling nozzle plate to the rearward to the right when viewed from the right side Lower coupling 4 20 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 6 Remove the 2 tube joints from the sampling nozzles Remove the tube of the left sampling nozzle from the tube clamp Hooks Tube clamp Tube joints 7 Lift up and pull the sampler unit toward you to unhook the hooks on the sampler unit from the tabs on the chassis 8 Rotate the sampler unit 90 counterclockwise when viewed from the right side and lift the unit up Be careful not damage sampling nozzles cables and tubes Service Manual MEK 6
122. ennen nnne nennen nnne 2 7 Reducing Background NOISE e A 2 8 Background Noise Problems acce o duin cen dea caca de tecta Dune din 2 9 Checking the Reproducibility s sisien ana a cnn a aada rca 2 9 Checking Procedure vecina ida 2 9 MON NANOS P c Ec 2 10 Checking ACCULACY EE 2 11 Checking Procedule iid tee trie eit E De diae ed dee ld 2 11 Poor WBC Reproducibility sseessssesseeseeeeeseeeneeeenneneeennnene nennen nnne rare ra anakaa niknais 2 11 Poor HGB Reproducibility RN 2 12 PoorRBG R producibiliby scroll sti 2 13 POOF PET REDIOGUCIDIINY cia eL EE 2 14 Poor HCT or MCV Reproducibility oooonnocccnnnccccnnncccconanccnonancnnonnncnnnnnn no nonnn nan nnne nnne nnne nennen nnne 2 15 Solving Problems from Alarm Messages sssssssssssseeeeeeeeenee nennen nennen nennen nnne nnne nnns senes 2 16 A901 NO dIIUerib Castalia 2 16 A005 No detergent c 2 16 A007 No lysing reagent sessssssseenennnnm eene nme nnrnn nennen nnne rennen nennen nenne 2 17 A009 WBO priming Error cinta credat uite ee been reete a eC Flu sace EIER EE di 2 17 A010 RBC PRIMING ERROR 2 pad rne an tto ee ergo tuere bp a Eee pecca pact ER erede 2 17 A021 WBC level T giiia aie ae aaa a us en x pra ua esa rey rua Pe aaa aiant 2 17 A022 WBC level Luarca 2 18 A029 WBG level caida tii ita 2 18 A024 WBO bu bble liviana ti in
123. ento eite ibo 4 3 Turning the Power Off spcis iien naraenia a oak r Eedi adaa 4 4 Cleaning and Draining the Fluid Pathway ooococonccccnnoccccononcnnonannnnnnnanonnananancnnn cnn 4 4 AMANING the PO WE Off ON 4 5 Removing the Right Side Cover Top Cover and Rear Cover ssssssssess 4 6 Reattaching the Right Side Cover Top Cover and Rear Cover 4 7 Removing the Front Panel Unit sssssseeennnm emnes 4 8 Reattaching the Front Panel Unit eeessssseeeeneenm mn 4 9 Installing the WA 640VK Printer Unit Option oooconccccnnnncccnnccconnnnccnnnnnrncnnnnrnan narco 4 10 Removing the Measuring Unit essseeen mme nnn 4 12 Reattaching the Measuring Unit coocconnonccccncnnnnoccccncccnnnonnncnnnonanennncnnnnnanenancnnnnnns 4 13 Accessing the Connectors on the POWER Board sssssssseeeene 4 14 Reassembling the Board cccccccceseccceeeeeeeeeneeeeeeeeneeneeeeeeeeneneeeeeeeenseeseeeeenenes 4 14 Removing the Combination Syringe Pump Unit eesesseeeeennee 4 15 Reattaching the Combination Syringe Pump Unit ssssseeess 4 16 Removing the Pump nil iier itii et te ct nente r ha EE Eee retaa idiatsaedeiiee 4 17 Reattaching the Pump Unit Quien a da oia 4 18 Removing the Sampler Unit ssssssseseseseseeeeeeeenneee nennen nennen nnns 4 19 Service Manual MEK 6400 6410 642
124. er press the RESET key for Rinse unit and Cap pierce nozzle on the OPER HISTORY screen to reset the counts to zero Cap pierce nozzle 81 Cp 9 Fill in the Maintenance Check Sheet 6 28 Service Manual MEK 6400 64 10 6420 6 MAINTENANCE Every Four Months Every 3 000 Counts Maintenance Procedures Checking and Cleaning Replacing the Sampling Nozzles Check and clean the sampling nozzles once every four months or every 3 000 sample counts whichever comes first When PLT background count increases or the sampling nozzle is bent replace the sampling nozzles with a new one WARNING The sampling nozzles are sharp and potentially contaminated with infectious materials Be careful when handling the sampling nozzles and performing this procedure Materials Required Powder free gloves lab coat safety glasses Phillips and flat blade screwdrivers Cotton swabs e CLEANAC 3 detergent Lint free pad New sampling nozzle s when required code no YZ 0341 Procedure 1 Do the procedure in the Before Maintenance Procedure earlier in this section to turn off the instrument 2 Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then toward you to remove the cover Service Manual MEK 6400 6410 6420 6 29
125. er turns off without cleaning Check that the power lamp is off 2 Remove the 3 screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then outward to remove the cover from the chassis 4 Remove the screw on the HGB cover to remove the HGB cover HGB cover 5 Turnon the instrument by pressing the Power key Service Manual MEK 6400 6410 6420 5 3 5 ADJUSTMENT READY M HOY 04 05 15 27 ID 0001 Qiu Sample type Dilute mode Sampling nozzle position RESULTS 5 4 6 On the READY screen press the MENU key OTHER key SENSOR MONITOR key to display the SENSOR MONITOR screen ENU M HOY 04 05 16 21 Q HOY 04 05 16 40 OPER HISTORY Ya DAILY OUTPUT CIRCUIT CHEG MAINTENANCE GD up BACKGROUND gt lt Electrode gt HGB 1 8824 1 8954 WBC 0 064 RBC 0 014 lt Mano Reagent gt WBC manometer RBC manometer Diluent Lysing reagent LEDOn LED 1 995 0 114 17 7 18 39 17 7 18 30 Upper Lower 0 844 1 244 4 299 7 1 184 2 414 2 294 lt 1 5 Y With reagent Without reagent gt 3 5 Y lt Temperature gt HGB unit MC unit Power board 27 0 C 36 4 C 32 4 C OK 7 Check that the following values are displayed If
126. erial data communication when the bar code port is used as RS 232C port Use handy bar code reader Check that a check mark is place in the check box aside of Yes when using a handy bar code reader The default setting is Yes Use specified cable Press the check box aside of Yes when using this port as RS 232C port with the YZ 0319 cable The default setting is No 2 37 2 TROUBLESHOOTING UNIT CAL Screen This screen allows you to change the compensation values of the cylinder HOY 04 05 16 21 volume of the MD 640V combination syringe pump unit and manometer volumes for RBC and WBC of the MC 640V measuring unit When replacing ES olo DILUTER E the MD 640V or MC 640V unit with a new one the change of the compensation value is not necessary because the compensation value of the HC WBC 35 new unit is automatically registered When you replace the board of the MD 640V or MC 640V unit i e UT 7200 or UT 7198 with a new one you need to change the compensation value Before the board replacement write down the compensation value Enter it after the board replacement MONITOR Screen HOY 04 05 16 21 This screen allows you to check the output voltage from each sensor alarm log and system error log ALARM LOG YSTEM ERR LOG OK SENSOR MONITOR screen Displays the status of all sensors with voltage or temperature For details on the INPUT CAL key refer to Section 5
127. es Phillips and flat blade screwdrivers Dry cloth or tissue paper e CLEANAC 3 detergent Microscope Procedures For daily cleaning of the aperture caps press the zy Clean key on the front panel However if the CLOG message frequently appears or the background count is high clean the aperture caps as directed in the following procedure NOTE The aperture caps are behind the measurement baths AA MOV 09 05 18 50 PRIME DRAIN BATHS Gu Gm CU Gia 1 Replace the CLEANAC detergent with CLEANAC 3 detergent 2 Pressthe STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning Refer to Before Maintenance Procedure earlier in this section 3 Replace the CLEANAC 3 detergent with CLEANAC detergent 4 Remove the diluent tube from the ISO3 diluent inlet and the hemolysing reagent tube from the HEMO3N inlet on the right side panel 5 Remove the detergent tube from the detergent container and put it into the waste container Do not disconnect the waste fluid tube from the WASTE outlet NOTE Waste comes out from the CLN inlet when DRAIN ALL is performed 6 Press the DRAIN ALL key on the OPERATIONS screen Waste container HOY 09 05 18 50 PRIME DRAIN BATHS _ o gt CAMA Cu qo 6 36 Service Manual MEK 6400 6410 6420 6 MAINTENANCE A confirmation message appears on the screen JPEH HOY 09 05 19 25 1 Remove CLN tube from detergent container a
128. etes all the above mentioned data This screen allows you to download the demonstration data from the PC memory card when inserting the memory card into the slot at the rear panel of the instrument and pressing the DOWNLOAD DATA key No function Service Manual MEK 6400 6410 6420 Section 3 Board Unit Description Board and Unit LOCATON c E EE 3 3 Right Side Vi Wicca 3 3 Leit SIDS Vie W siii ira il dd cord id 3 3 Front ViSW sasao EET 3 4 TOP VOW ir A A E 3 4 MC 640V Measuring Unit eesesssssssesesessee senem eee dee deena iiaea riiai aaa aiai 3 5 MD 640V Combination Syringe Pump Unit s sisien naaa nennen ener e nnne srt rins entren nnn 3 5 MP 640V PUMP UN m S 3 6 PV 641V Front Panel Unit C 3 6 JO 640V Ode M EE 3 7 JQ 641V JQ 642V Valve Unit 52 iones estet oec id t Rug dde acd occae 3 7 Nec RM ATCTAUMIROMT REP 3 7 J9 642V Valve UN o o III MEE tn MET N ec Lao 3 7 MS 640V Sampler Unit essssssssssesseseeessee nennen nennen da eaa AR NAR i eaaa dea aAa tnnn seen EETA sea kanaa 3 8 MS 641V Cap Pierce Unit MEK 6400 only ooooooconoccccncoccccnoncccnnoncncnnonnncnnnnnnnnnonnncn non n nn nannn kaanak anaiai iiias 3 8 MR 640V Rinse Unit MEK 6410 6420 only ooooocococcccccoccccnoonncncancncnnnnnncnnnnnnnnnnnnnnnnann cnn na nnns nn tnne stent Aaa a 3 9 UT 7192 AMP CONTROL Board sse nennen
129. ge hardware version total operating time hours and total number of counts HOY 04 05 16 40 Software version V01 0 1450 Language JAPANESE ENGLISH Hardware version 6400 Y01 02 Total measurement count 1283 counts Total operating time 151 hours The second page displays the number of counts for filters measurement baths sub baths pump tube rinse unit sampling nozzles and cap pierce nozzle HOY 04 05 16 40 Filters baths amp sub baths rn 83 3000 3338 E Pump tube Rinse unit Sampling nozzle Cap pierce nozzle 61 1000 scam OK PREY NEXT After checking and replacing the filters measurement baths sub baths pump tube rinse unit sampling nozzles and cap pierce nozzle reset the counts to zero by pressing the RESET key 3 Press the OK key to return to the OTHER screen Service Manual MEK 6400 6410 6420 6 7 6 MAINTENANCE Maintenance Check Sheet Copy fill out and save this check sheet each time you do maintenance or service Checking Date Customer Customer Address Service Personnel Service Company Analyzer Model Analyzer Serial Number Hardware Revision Number Software Revision Number Check Item OK No Daily Check There are enough Nihon Kohden specified reagent recording paper and other consumables ISOTONAC3 diluent CLEANAC detergent CLEANAC3 detergent HEMOLYNAC3N lysing reagent
130. ge pump for hemolyzing reagent which aspirates the hemolyzing reagent from the reagent bottle and sends it to the WBC measurement bath Service Manual MEK 6400 6410 6420 3 5 3 BOARD UNIT DESCRIPTION MP 640V Pump Unit This unit creates a negative pressure to aspirate diluted blood through each aperture and to drain the waste fluid This unit creates a positive pressure to completely mix the diluted blood sample and hemolyzing reagent for hemolyzation PV 641V Front Panel Unit This unit has key switches and LCD with touch screen It may also have an optional printer unit The LCD displays the measurement result numeric data and histogram and alarm message if something wrong occurs The measurement result can be printed if an optional printer is installed 3 6 Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION JQ 640V Inlet Outlet Unit This unit has inlet ports for diluent detergent and hemolyzing reagent and an outlet port for waste fluid The three liquids which are detected with liquid sensors of this unit are supplied to the related units through the fluid path The waste fluid is drained through the outlet port JQ 641V JQ 642V Valve Unit JQ 641V Valve Unit This unit is for the MEK 6400 analyzer It has six 2 way valves and a fluid path to send the diluent or detergent to the other units JQ 642V Valve Unit This unit is for the MEK 6410 MEK 6420 analyzers It has four 2 way valves
131. ibration coefficients on the CALIBRATION screen for closed open and pre dilution modes When the X R values are outside the range in the previous check measure the MEK 3DN hematology control 3 times on the CALIBRATION screen and write down the mean values and calibration coefficients If the measured data of one measurement is extremely different from the other two measurements delete that data and only use the mean of the two measurement data which are close to each other For details on how to measure the hematology control on the CALIBRATION screen refer to Section 7 Calibration in the Operator s Manual Checking the New Calibration Coefficients For details on how to measure the hematology control on the CALIBRATION screen refer to Section 7 Calibration in the Operator s Manual 1 Measure the MEK 3DN hematology control 3 times on the CALIBRATION screen for closed mode Write down the lot number of the measured hematology control If the measured data of one measurement is extremely different from the other two measurements delete that data and only use the mean of the two measurement data which are close to each other Service Manual MEK 6400 6410 6420 6 43 6 MAINTENANCE Checking the Prime Function sd ACI HOY 09 05 19 50 Gaia CU GD ELA REMOVE CLOG STRONG CLEA Checking the Drain Function Checking the Cleaning Function 6 44 In the lt Data gt field enter the median of the assay val
132. id path and recount the diluent If the values are still not optimum refer to Section 2 Troubleshooting The diluent is clean No bubbles in the diluent The aperture caps are clean The aperture caps are firmly attached The measurement baths and sub baths are clean Measuring Hematology Control 6 20 Measure a MEK 3D hematology control which has the same conditions as human blood Confirm that the obtained sample data is within the acceptable range on the assay sheet attached to the hematology control CAUTION Use hematology control before the expiration date Do not use hematology control when the top layer is slightly red or the whole hematology control is red because the red blood cells in the control are hemolyzed Do not freeze the hematology control because this hemolyses it Use and store the hematology control with extreme care according to its instructions 1 Gently take out the hematology control from the refrigerator 2 Bring to room temperature by rolling the hematology control vial between the palms of the hands 3 Turn the hematology control vial upside down at least 30 times to thoroughly mix the plasma and red blood cells 4 Select Open for Sampling mode on the READY screen 5 Put the sampling nozzle into the bottom of the hematology control vial so that the tip of the sampling nozzle touches the bottom of the vial 6 Press the lt gt
133. ing down the sampling nozzle and turn the lower coupling to adjust the position of the sampling nozzle sideways front and back If necessary adjust the cap pierce unit or rinse unit position 4 24 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the Inlet Outlet Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the 2 tube joints from the inlet outlet unit and 1 tube joint from the pump unit Inlet outlet unit Pump unit 4 Remove the cable connector from the inlet outlet unit al RR RN Hook 5 Remove the 2 screws which secure the inlet outlet unit to the chassis Service Manual MEK 6400 6410 6420 4 25 4 DISASSEMBLY AND ASSEMBLY 6 Lift the inlet outlet unit up to unhook the hook from the chassis and pull out the unit toward you Reattaching the Inlet Outlet Unit To reattach the inlet outlet unit reverse the above procedure Removing the Valve Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minu
134. ing the Reagents Connection Tubes ooooccccnoccccnncccccononccnonn cnn nono ncn nono conan rra naar cnn r nnns entren nnns 6 16 Checking the Power Cord and Grounding Lead sss eene enne nnne nnns 6 16 Checking the External Instrument Connection ssessssssssesseseeeeeeeene nennen nnns 6 16 eae xepurdrgem E 6 17 Calibrating the TOUGH SCIO o dae e osepedee aid diia 6 17 Checking the Date and TIME RE lidia 6 17 Clock verna aia 6 18 MEU nmm nennen nnne nnn nnhhenrn nennen nnne nnne nnne 6 18 Measuring EME nennen nennen enitn nnn EAE a Aa EES 6 20 Checking Measurement Baths and Sub Baths ssssssssssssssssseeeeeeeeene nennen nennen nnns 6 21 Checking PUMP TUDE m MQ 6 21 Weekly Every 300 Counts Maintenance Procedures ococccooccccncocccnonoccccnnnnnnnnnnnnnnono cnn ran nn cnn nn rn enne entente 6 21 Checking GleaningFilterS scooters ca 6 21 Monthly Every 1 000 Counts Maintenance Procedures oococcoccconocccconoccnconnccononancn nono ncn non cnc cnn ona rann nc nnne nennen 6 22 Replacing FINOS inisiasi ESEE do 6 22 Materials Required APPO S 6 22 Procedur oe PEE 6 22 Checking and Cleaning Measurement Baths and Sub Baths ooooocccninccccinnccccononnnnnononanonancnnnnnn cnn nana nn cnn 6 23 Materials ROQUITSO E a 6 23 PFOCCCUIG ion A ct ea 6 24 Checking and Cleaning Replacing the Rinse Unit and Cap
135. it with a new one Electromagnetic valve failure Replace the UT 7192 AMP CONTROL board with a new one Replace the UT 7193 POWER board with a new one A023 WBC level 3 This message appears when the manometer lower sensor does not detect the liquid during WBC measurement or aspirating sample Reason Possible Cause Criteria Countermeasure pressure Cannot produce measuring The pump tube is damaged Replace the pump tube with a new one The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure can be produced but there is leakage WBC aperture is not attached properly Reattach the WBC aperture properly The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure can be produced but there is clog WBC aperture is clogged Clean or replace the aperture with a new one The MC 640V measuring unit failure Replace the unit with a new one A024 WBC bubble 1 This message appears when the manometer upper or lower sensor detects no liquid during WBC measurement or at the start of draining manometer Reason Possible Cause Criteria Countermeasure Diluent cannot be detected even when there is enough WBC manometer is dirty Perform Strong Cleaning WBC manometer upper sensor adjustment error Adjust the sensor Refer to Adjusting the Upper and Lower Sensor Output Volt
136. ith CLEANAC 3 detergent If the cap pierce nozzle is damaged or dirt blood cannot be removed replace the cap pierce nozzle with a new one O ring for rinse unit and cap pierce nozzle upper code no 295538 O ring for cap pierce nozzle guide lower code no 315366 Cap pierce nozzle bottom view seen from the front panel side with the tube holder opened Service Manual MEK 6400 6410 6420 6 27 6 MAINTENANCE To replace the cap pierce nozzle 1 Turn the cap pierce nozzle 90 counterclockwise 2 Remove the joint from the cap pierce nozzle 3 Pull the cap pierce nozzle up to remove it 4 Reattach the joint to the new cap pierce nozzle 5 Insert the cap pierce nozzle into the cap pierce nozzle guide and turn the cap pierce nozzle 90 clockwise Cap pierce nozzle guide 6 Reattach the right side cover and fasten it with the three screws on the rear AA MOV 04 05 16 40 Filters baths amp sub baths 93 1000 ss panel and one screw on the right side panel 7 Press the Power key to turn on the power The analyzer starts priming the Pump tube 83 3000 33 fluid pathway Rinse unit 834 100 3331 8 If the rinse unit and cap pierce nozzle were checked and cleaned the rinse Sampling nozzle 83 3000 3300 unit and cap pierce nozzle counter will have to be reset To reset the count
137. ition for dispensing the diluted sample for WBC measurement RBCASPPOS Moves the sampling nozzle to the position for aspirating the diluted sample for WBC measurement RBCDSPPOS Moves the sampling nozzle to the position for dispensing the diluted sample for RBC measurement 1CYCLE Moves the sampling nozzle to the positions according to each operation of MS 640V CONT INITIAL Repeats moving the sampling nozzle to the initial position until you press the Reset key on the front panel 2 33 2 TROUBLESHOOTING IHITIALIZE HIGH POS LOW POS Gs Qs 2 34 HOY 04 05 16 21 MS 641V MEK 6400 only This screen allows you to check the operation of the MS 641V CAUTION If you check the operation while the tube holder is open the cap pierce needle comes out Always perform INITIALIZE before pressing any other key INITIALIZE Moves the cap pierce needle to the initial position HIGH POS Moves the cap pierce needle to the upper position LOW POS Moves the cap pierce needle to the lower position MV amp PUMP CHK This screen allows you to check the operation of the valves and MP 640V pump unit CAUTION Before starting the pump carefully check what happens by opening a specified valve and working the pump Otherwise the reagent such as diluent may overflow somewhere in the instrument When pressing a check box of MC 640V MD 640V JQ 640V and JQ 641V
138. k that no cable is pinched 8 Put the front panel unit back to the original position 9 Fasten the front panel unit to the chassis with the 4 screws 10 Connect the 2 cables of the front panel unit to the AMP CONTROL board 11 Connect the cable of the printer unit to the AMP CONTROL board Front panel unit cables Printer unit cable 12 Connect the cable of the front panel unit to the POWER board Refer to step 4 in the Removing the Front Panel Unit section 4 13 Attach the printer door to the printer unit on the front side of the front AS panel unit while depressing the stopper downward and pushing the printer door into the opening of the printer unit Service Manual MEK 6400 6410 6420 4 11 4 DISASSEMBLY AND ASSEMBLY Removing the Measuring Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Disconnect the 3 cables from the AMP CONTROL board Pyer a Wee Screw 4 12 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Remove the 4 tube joints and sub bath motor cable c
139. l potential difference between the instruments The potential difference between the instruments may cause current to flow to the patient connected to the instruments resulting in electrical shock micro shock Always perform equipotential grounding when required It is often required in the operating room ICU room CCU room cardiac catheterization room and X ray room Consult with a biomedical engineer to determine if it is required When equipotential grounding is required connect the equipotential ground terminal on the rear panel of the analyzer to the equipotential ground terminal on the wall equipotential grounding system with the equipotential grounding lead potential equalization conductor Service Manual MEK 6400 6410 6420 9 INSTALLATION Connecting Tubes and Installing Reagents In order for the analyzer to operate correctly you must install all reagent and waste tubing before the power is turned on Materials Required Powder free gloves lab coat safety glasses ISOTONAC 3 diluent CLEANAC detergent CLEANAC 3 detergent Hemolynac 3N lysing reagent Reagent inlet tubings and waste outlet tubing Waste container or appropriate drain 2 L container Lint free cloth CAUTION Only use Nihon Kohden recommended reagents Otherwise the measurement result cannot be guaranteed and incorrect reagent concentration can cause equipment damage NOTE e Only use the CLEANAC 3 detergent for st
140. lding down the Reset key The power turns off without cleaning Check that the power lamp is off FA NIHON KOHDEN 6 14 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Daily Maintenance Procedures Checking Reagents and Other Consumables CAUTION Use only Nihon Kohden recommended reagents Otherwise the measurement result cannot be guaranteed and incorrect reagent concentration can cause equipment damage Check that the following Nihon Kohden specified reagents are used e ISOTONACS3 diluent CLEANAC detergent e CLEANAC 3 detergent For STRONG CLEAN only e Hemolynac 3N hemolysing reagent Check that these reagents are not expired and are not run out Check that there are enough consumables such as hematology control sample containers sample tubes and sample cups When using a printer check that there is enough recording paper Checking the Appearance of the Analyzer Check the following points There are no damaged or dirty parts on the outside of the analyzer The sampling nozzle switches and keys are not damaged There is no leakage from the analyzer The labels are not torn or removed WARNING Be careful not to directly touch any place where blood is or may have contacted Protect yourself from infection before cleaning and doing maintenance CAUTION Turn off the main power before disinfection or cle
141. leaning or replacing the cap pierce needle Service Manual MEK 6400 6410 6420 HOY 04 05 16 21 Low High WBC 2 0 5 0 RBC 2 00 7 00 HGB 6 0 20 0 HCT 20 0 60 0 MCV 50 0 150 MCH 20 0 40 0 MCHC 25 0 40 0 PLT 30 600 e e OK HOY 04 05 16 21 Anisocytosis PLT clumps 300 Pit Rbcinterfere 0 10 BAR CODE PORT Use handy bar code reader HOY 04 05 16 21 Y ves No Use specified cable O Yes f No A a OK Ta Ey Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING PANIC VALUE Screen for MEK 6400 and MEK 6410 This screen allows you to change the upper and lower thresholds for remeasurement with the dilution ratio changed When a blood sample has a parameter which is out of the range between the upper and lower thresholds the instrument automatically measures the sample with the dilution ratio changed Changing the upper and lower thresholds depends on the user s needs FLAG SETTINGS Screen This screen allows you to change the thresholds for display of the following specified flags If there are a lot of normal blood samples which have one of the following flags frequently displayed increase the value to prevent displaying the flag The initial value is 20 0 RDW The initial value is 300 The initial value is 0 10 Anisocytosis PLT clumps PIt Rbc interface BAR CODE PORT Screen This screen allows you to set the settings for s
142. least every 6 months 2 If stored for extended periods without being used make sure prior to operation that the instrument is in perfect operating condition Service Manual MEK 6400 6410 6420 i 9 When the instrument is used with an electrosurgical instrument pay careful attention to the application and or location of electrodes and or transducers to avoid possible burn to the patient 10 When the instrument is used with a defibrillator make sure that the instrument is protected against defibrillator discharge If not remove patient cables and or transducers from the instrument to avoid possible damage WARRANTY POLICY Nihon Kohden Corporation NKC shall warrant its products against all defects in materials and workmanship for one year from the date of delivery However consumable materials such as recording paper ink stylus and battery are excluded from the warranty NKC or its authorized agents will repair or replace any products which prove to be defective during the warranty period provided these products are used as prescribed by the operating instructions given in the operator s and service manuals No other party is authorized to make any warranty or assume liability for NKC s products NKC will not recognize any other warranty either implied or in writing In addition service technical modification or any other product change performed by someone other than NKC or its authorized agents without prior consent of NKC m
143. ll 4 values are less than 1 5 V On the MENU screen press the OPERATIONS key gt DRAIN ALL key to drain the fluid from the manometers Follow the instructions on the screen Make sure that the tubes are correctly connected Repeat steps 4 and 5 so that all values become above 3 5 V When the upper and lower sensor output voltages of the manometers are correctly adjusted return the type of user to the previous type Service Manual MEK 6400 6410 6420 5 ADJUSTMENT Adjusting the Liquid Sensor Output Voltages The JQ 640V inlet outlet unit has liquid sensors for detecting the presence or absence of diluent detergent and hemolysing reagent When there is reagent in the instrument the sensor voltage should be less than 1 5 V When the instrument is empty the sensor voltage should be more than 3 5 V The liquid sensors detect the presence or absence of reagent in the instrument G based on the borderline sensor voltage of 2 5 V If the sensor voltage is not correct the sensors cannot detect the reagent in the instrument and No diluent No detergent or No lysing reagent alarm may occur 1 The type of user must be Service to enter the SERVICE screen i On the MENU screen press the SETTINGS key USERS key SETTINGS ME HOY 04 05 14 40 m ED E DATE amp TIME FAUTO CLEANING DISPLAY K USERS D OUTPUT HOY 04 05 16 21 emm A CALIBRATION Ee DATA OPERATIONS OTHER ecm INIT
144. lve unit Sampler unit Inlet Outlet unit Combination syringe pump unit Pump unit Front Panel unit Cap pierce unit MIXED PUMP board MEK 6400 only Measuring unit Left Side View AMP CONTROL board MEASURING board Service Manual MEK 6400 6410 6420 3 3 3 BOARD UNIT DESCRIPTION Front View LCD KEY board PRINTER DRIVER board Top View LIQUID SENSOR board POWER board AMP CONTROL board 3 4 Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION MC 640V Measuring Unit When a blood cell of the diluted blood sample passes through the aperture the resistance between the external and internal electrodes increases This change in resistance causes a proportional change in voltage because a constant current flows between the electrodes This measuring unit counts the blood cells by the voltage changes Meanwhile the total diluted blood sample volume which is aspirated through the aperture is kept constant by the manometer of this measuring unit At the lower part of the WBC measurement bath a light of 540 nm wavelength shines through the hemolyzed blood sample The sample absorbs some of the light A photodiode detects the unabsorbed light The HGB can be determined from the light absorption ratio of the sample and diluent MD 640V Combination Syringe Pump Unit This unit dilutes the blood sample 200 times and sends the diluent to each part of the fluid path This unit has another syrin
145. ly indicated by an increase in imprecision of one or more of the directly measured parameters This imprecision is due to carryover or dilution sampling inconsistencies If this occurs on more than a random basis perform the appropriate maintenance more frequently than indicated Follow your local laws for disposing of medical waste WARNING Dispose of the blood sample and replaced parts by following your local laws for disposing of infectious medical waste Always wear rubber gloves to protect yourself from infection when handling waste Repair Parts Availability Policy Nihon Kohden Corporation NKC shall stock repair parts parts necessary to maintain the performance of the instrument for a period of 7 years after delivery of the instrument In that period NKC or its distributors will repair the instrument This period may be shorter than 7 years if the necessary board or part is not available For discontinuation announcements contact your Nihon Kohden distributor or representative Parts to be Replaced Periodically 6 4 Filter code no T802 3 pcs for MEK 6400 2 pcs for MEK 6410 6420 Pump tube code no T462 1 pc Service Manual MEK 6400 6410 6420 6 MAINTENANCE Maintenance Schedule Checking cap pierce nozzle is only necessary on the MEK 6400 analyzer Service Manual MEK 6400 6410 6420 Perform the following procedures at the scheduled intervals You can use the OPER HISTORY screen t
146. measurement bath and the round indent of the measurement bath fits the aperture 20 Tighten the screws which were loosened in step 13 to fasten the measurement baths NOTE Before tightening the screws check and remove any dirt or rust on and around the screws If dirt or rust is present noise alarm may occur during measurement Round indent Reconnect the filter joints to the RBC and WBC measurement bath assemblies by turning the tube connectors if they were removed in step 14 2 Reattach the tube joint to the WBC measurement bath by turning the knurl joint 22 Reattach the HGB cover and fasten it with the screw 23 Reattach the right side cover and fasten it with the three screws on the rear panel and one screw on the right side panel 24 Press the Power key to turn on the power The analyzer starts priming the fluid pathway 25 Fill in the Maintenance Check Sheet Cleaning the External Electrodes on the Measurement Baths The external electrodes are the screws on the left side of each measurement bath Check that these screws are not dirty or rusty If there are dirt or rust on the external electrodes they may cause NOISE alarm When the screws are dirty or rusty remove them by cleaning the measurement bath with the CLEANAC 3 detergent Refer to the Checking and Cleaning Measurement Baths and Sub Baths earlier in this section Service Manual MEK 6400 6410 6420 6 39 6
147. ment baths and sub baths 1 Do the procedure in the Before Maintenance Procedure earlier in this section to turn off the instrument 2 Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 3 Slide the right side cover to the rear direction and then pull it toward you to remove the cover 4 Remove the screw on the HGB cover to remove the HGB cover HGB cover 5 Check the WBC and RBC measurement baths and sub baths If there is any blood or dust on them remove and clean them taking the following steps 6 24 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Steps 10 11 Step 6 6 Remove the tube joint connected to the WBC pav baths WBC meas bath measurement bath by turning the knurl joint Tube joint 7 Remove filter joints on the RBC and WBC measurement bath assemblies by turning the tube connectors 8 Loosen the screws fastening the measurement baths The screws cannot be removed from the measurement baths I2 7j e B y UH RID lee ALAR
148. ming Voltage adjustment error of the liquid sensor Adjust the voltage Refer to Adjusting the Liquid Sensor Output Voltages in Section 5 Liquid sensor failure Replace the UT 7199 LIQUID SENSOR board with a new one Wrong tube The cleanac tube 8 tube for CLEANAC3 is used for diluent Use the diluent tube A005 No detergent This message appears when the liquid sensors on the JQ 640V inlet outlet unit do not detect the detergent during priming or cleaning Reason Possible Cause Criteria Countermeasure No detergent The tube between the hematology analyzer Check the tube connection and if necessary replace flow and CLEANAC detergent is squeezed bent the tube with a new one clogged or there is leakage Pump tube is damaged Replace the pump tube with a new one The MC 640V measuring unit failure Replace the unit with a new one The MP 640V pump unit failure Replace the unit with a new one Failure of the sensor in the tank Air bubbles on the liquid sensor Perform priming Voltage adjustment error of the liquid sensor Adjust the voltage Refer to Adjusting the Liquid Sensor Output Voltages in Section 5 Liquid sensor failure Replace the UT 7199 LIQUID SENSOR board with a new one 2 16 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING A007 No lysing reagent This message appears when the sensors on th
149. molyzing reagent EJE XP 602V XP 612V 2 way Electromagentic Valve These valves open or close the fluid path when receiving the valve drive voltage from the UT 7193 POWER board XP 602V valve has a silicone rubber diaphragm XP 612V valve has a fluorocarbon rubber diaphragm Service Manual MEK 6400 6410 6420 3 13 4 DISASSEMBLY AND ASSEMBLY Section 4 Disassembly and Assembly BSOS UB iii iii 42 Warnings and Caution rro od 4 2 FREQUITOR TOONS uc ds 4 2 Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the Instrument 4 3 Board and Unit Location aa coin ir A AAA 4 3 Turning the Power Off PE 4 4 Cleaning and Draining the Fluid Pathway sessssssseeeeeeeeeeeeeneeeen nennen nennen nnne nnn nnn nnns 4 4 TUNING the eo pe 4 5 Removing the Right Side Cover Top Cover and Rear Cover sssssssssseseeeeneeeeeen eene nnne 4 6 Reattaching the Right Side Cover Top Cover and Rear Cover sssssssssseeseeeneennene 4 7 Removing the Front Panel Unit iiec ctore a a eens x Ee eue d uta 4 8 Reattaching the Front Panel Unit canica etae ree eta s tema cope sta sd ae ayuu n dra be uan can Enea nona RE us 4 9 Installing the WA 640VK Printer Unit Option ssssesssseseeeeneenenennneeennneennne nennen nnn nnne nennen 4 10 Removing the Measuring AA nennen eene nnnnnnne enne nnnnnnnn nennt enennn nnn nnne nennen 4 12 Reattaching the Measuring Uni
150. move all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover and top cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove all cable connectors from the AMP CONTROL board 4 Remove the 3 screws which secure the AMP CONTROL board from the rear cover e EE 4 28 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Remove the 7 screws which secure the AMP CONTROL board to the chassis 6 Pull the AMP CONTROL board to the right then toward you to remove it from the instrument Reattaching the AMP CONTROL Board To reattach the AMP CONTROL board reverse the above procedure Service Manual MEK 6400 6410 6420 4 29 4 DISASSEMBLY AND ASSEMBLY Removing the POWER Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Do the procedure in Accessing the Connectors on the POWER Board earlier in this section to open the POWER board 3 Remove the 3 cable c
151. mple time is too short from lower sensor ON to upper sensor ON Reason Possible Cause Criteria Countermeasure The aperture is too WBC aperture is damaged Replace the WBC aperture with a new one large Incorrect aperture 100 um Use an appropriate aperture 80 um Air bubbles in the No liquid Perform priming manometer The MC 640V measuring unit failure Replace the unit with a new one A029 WBC clog This message appears when the manometer lower sensor detects the liquid but the upper sensor does not detect the liquid during WBC measurement or aspirating sample Reason Possible Cause Criteria Countermeasure Measuring pressure can WBC aperture is clogged Clean or replace the WBC aperture with a new one be produced but there EE I is clog The MC 640V measuring unit failure Replace the unit with a new one Cannot produce measuring pressure The pump tube is damaged Replace the pump tube with a new one The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure can be produced but there is leakage WBC aperture is not attached properly Reattach the WBC aperture properly The MC 640V measuring unit failure Replace the unit with a new one Measuring at high altitude higher than 1 000 m above sea level High altitude mode needs to be set to On Set High altitude on ADVANCED SET SETTINGS MENU of the S
152. n 6 21 Monthly Every 1 000 Counts Maintenance Procedures ooooccconccccconocccconcccnnonnnccnannncanonnos 6 22 Replacing FIters ierra brink AO 6 22 Materials Required c 6 22 gem 6 22 Checking and Cleaning Measurement Baths and Sub Baths 6 23 Materials Required mini 6 23 disco pa EE 6 24 Checking and Cleaning Replacing the Rinse Unit and Cap Pierce Nozzle 6 26 Materials Required cocoa 6 26 deos PEE 6 27 Every Four Months Every 3 000 Counts Maintenance Procedures 6 29 Checking and Cleaning Replacing the Sampling Nozzles sssss 6 29 Materials Required eeeesesessseseeeseeeeee enne nnne nnn nnn 6 29 ure gm 6 29 Replacing Pump Tube ooooocccccccononoccccccnonnnnncnnnnnnnnnncnnnncn nano eene nennen 6 32 Materials Hequlited zi ro tie xt peii oe iere oues 6 32 PYOCCOUIC m deedean 6 32 Every Six Months As Required Maintenance Procedures cooccccccnocoocccnccoonoonncnncccnanonoss 6 35 Removing a Clog from the Aperture sssssssseseeeneneeeenneeenn nenne 6 35 Cleaning Aperture Caps ze itt etc rete eet re doit eee 6 36 Materials Hequlted 5 coit orit tirer tac ates eb ooi tea pares ur pi eeu 6 36 Procedures D 6 36 Cleaning the
153. n pre dilution blood is measured data accuracy depends on careful clotting and diluting Before using a sahli pipette for pre dilution blood collection check that the sahli pipette is completely cleaned and dried An optional microcap 20 uL is recommended for pre dilution blood collection Supply code no T812 When putting the pre dilution blood into a sample cup make sure there are no bubbles in the cup If you cannot avoid making bubbles with the sahli pipette we recommend the optional microcap which prevents bubbling Service Manual MEK 6400 6410 6420 2 5 2 TROUBLESHOOTING n pre dilution mode about 1 mL of sample is aspirated Make sure that the sampling nozzle is near the bottom of the sample cup so that the correct volume of the sample can be aspirated 2 6 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING Checking the Hematology Analyzer There are two hematology analyzer checks background noise and reproducibility Checking Background Noise Measure the diluent to check the background noise Measurement Count the diluent in closed mode and open mode Closed mode is only for MEK 6400 1 Select Closed or Open for Sampling mode and press the MENU key on the READY screen 2 Press the OTHER key on the MENU screen 3 Press the BACKGROUND key on the OTHER screen The Measure background noise message appears on the screen 4 Press the YES key to measure backgroun
154. nd insert it into waste container 2 Disconnect ISO3 and HEMO3N tubes from inlets on the hematology analyzer NOTE Never remove tube from WAST outlet Drain all SENES NO 7 Press YES to start draining the analyzer NOTE Be sure all reagent has drained into the container Failure to do so may result in a liquid spill 8 After draining press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 9 Remove the three screws on the rear panel and one screw on the right side panel The two screws marked with A only need to be loosened and not removed 10 Slide the right side cover to the rear direction and then toward you to remove the cover HGB cover Service Manual MEK 6400 6410 6420 6 37 6 MAINTENANCE 12 Remove the tube joint connected to the WBC measurement bath by turning the knurl joint 13 Loosen the screws fastening the measurement baths The screws cannot be removed from the measurement baths
155. new one Liquid cannot be drained from the measurement bath Replace the filter below the RBC measurement bath with a new one Blood sampling error sampling nozzle is clogged bent or air bubbles in the sampling nozzle Replace the sampling nozzle with a new one 2 26 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or PLT Clumps appears Platelet aggregation is a phenomenon where some platelets stick together to create a large aggregated platelet cell This is because some platelets in the sample excessively react against anticoagulant When platelet aggregation occurs the PLT value is low and the insufficient hemolysing alarm also occurs because the aggregated PLT cell is counted as a WBC or RBC ghost There is a high possibility that platelet aggregation occurs when heparin is used as an anticoagulant Platelet aggregation rarely occurs when EDTA ethylenediaminetetraacetic acid salt is used This alarm is displayed when there is poor hemolyzation and PLT value is low Reason Possible Cause Criteria Countermeasure of the sample Specific characteristics PLT aggregation Measure with a microscope anticoagulant Error due to Heparin is used as an anticoagulant Collect the sample again and use EDTA as an anticoagulant EDTA dependent pseudothrombocytopenia Collect the sample again and use heparin as
156. not HGB sensor must be adjusted HGB LED On 3 00 1 V HGB LED Off below 0 5 V Service Manual MEK 6400 6410 6420 5 ADJUSTMENT 8 Adjust the HGB variable resistance volume on the measuring unit with a phillips screwdriver so that the HGB sensor voltage is within the appropriate range HGB variable resistance volume 9 Turn off the instrument and replace the right side cover Service Manual MEK 6400 6410 6420 5 5 5 ADJUSTMENT Adjusting the Upper and Lower Sensor Output Voltages of the Manometers VA NIHON KOHDEN bd HU 0 lame Type of user Other user Other user CHANGE Y ADD USER 3 The MC 640V measuring unit has manometer for WBC and RBC for sampling 360 uL of diluted sample accurately Each manometer has upper and lower sensors for detecting the presence or absence of liquid in the manometer When there is liquid in the manometer the sensor voltage should be less than 1 5 V When the manometer is empty the sensor voltage should be more than 3 5 V The upper and lower sensors detect the presence or absence of liquid in the manometer based on the borderline sensor voltage of 2 5 V If the sensor voltage is between 2 25 and 2 75 V the sensors cannot detect the liquid surface accurately and manometer dirty alarm may occur To correctly detect the presence or absence of liquid in the manometer the sensor voltage difference of at least 2 0 V i
157. nt Stir the sample thoroughly before measurement Inappropriate measurement settings Possible Cause Criteria Countermeasure appropriate The sensitivity and threshold settings are not Check that the sensitivity and threshold settings are set as below RBC SENSITIVITY 5 RBC THRESHOLD AUTO PLT THRESHOLD 5 Dilution error Possible Cause Criteria Countermeasure The MS 640V sampler unit failure Replace the sampling nozzle with a new one Replace the unit with a new one unit failure The MD 640V combination syringe pump Replace the unit with a new one 2 14 Service Manual MEK 6400 6410 6420 Hematology analyzer failure 2 TROUBLESHOOTING Possible Cause Criteria Countermeasure The aperture is dirty Clean the aperture The aperture is damaged Replace the aperture with a new one Circuit malfunction PLT is outside 160 5 when circuit check is performed by pressing MENU key gt OTHER key CIRCUIT CHECK key Replace the UT 7198 MEASURING board with a new one Replace the MC 640V measuring unit with a new one Replace the UT 7192 AMP CONTROL board with a new one The MC 640V measuring unit is not attached properly Reattach the unit Make sure to tighten the screws attaching the unit to the chassis Refer to Removing the Measuring Unit in Section 4 The MC 640V measuring unit failure Replace the unit
158. nt When this setting is set on and an alarm message such as bubble or noise occurs the instrument automatically measures the sample up to 3 times while displaying the alarm message for each measurement This function is useful to check what alarm occurs during the recount if the recount frequently occurs The default setting is off REAGENT FACTOR Screen This screen allows you to change the ratio of consumption of each reagent when there is a difference between the actual consumption and calculated consumption on the screen Diluent Detergent Changes the consumption rate of the diluent Changes the consumption rate of the detergent Lysing reagent Changes the consumption rate of the hemolysing reagent USER MAINTENANCE Screen This screen allows you to change the measurement times of the following items The instrument counts the measurement times When the actual measurement times exceeds the preset measurement times for check cleaning or replacement the corresponding user maintenance message appears Filters Baths Measurement times for checking cleaning or replacing the filter and measurement and sub baths Pump tube Measurement times for checking cleaning or replacing the pump tube Rinse unit Measurement times for checking cleaning or replacing the rinse unit Sampling nozzle Measurement times for checking cleaning or replacing the sampling nozzle Cap pierce nozzle Measurement times for checking c
159. nts essssssssssseeeeneeeeee rana 6 43 Checking the New Calibration Coefficients sssssssssseeeen eene nnne nnne nnns 6 43 Checking the Prime FUNCION eiie eren i eem reet torna nae n Rat ab eR ER ERR AER ERA Eaa a Eae paid 6 44 Checking the Drain FUNCION E 6 44 Checking the Cleaning Function oonoccconnccccnnicccnononccnonnancn coronan nn cr narrar carr 6 44 Checking the Dispensing Function ocooncccnnnncccnnoncccnnnnncnnnonacanonnn cnn nan n cnn nennen Ea a nennt nne a trennen nne RN 6 45 Checking the External Instruments Function eessessesssssseseeeseee nennen enne nenne nene nnne nnne nnns 6 45 illom X M 6 45 Hand held Bar Code Reader etia eu a Desa rente eine dai uR 6 45 ac ES 6 45 Checking the Power Cordi p 6 45 Checking the Resistance of the Protective Ground Line oooooocnnnncccnnnncocnnnoccnnnnncccnnncnnn nano nn nn conan 6 45 Checking the Earth Leakage Current oooccccincccccnncocccononcnnnonocanonnn cocoa n cnn nan n cana nn nennen nennen nnns nnne nnne nnns 6 46 Service Manual MEK 6400 6410 6420 6 MAINTENANCE General The scheduled maintenance procedures described in this section must be routinely performed in order to ensure optimum performance Failure to perform the scheduled maintenance procedures may result in inaccurate or imprecise analysis of whole blood samples This section describes the maintenanc
160. nulocyte count MEK 6400 6410 0 to 59 x 10 uL MEK 6420 0 to 99 x 10 uL RBC Red blood cell count 0 to 14 9 x 10 uL within 1 5 CV 5 0 x 105 uL width HGB Hemoglobin concentration 0 to 29 9 g dL within 1 5 CV 16 g dL HCT Hematocrit 0 to 99 9 MCV Mean cell volume 20 to 199 fL within 1 0 CV 70 to 120 fL MCH Mean cell hemoglobin 10 to 50 pg MCHC Mean cell hemoglobin 10 to 50 g dL concentration RDW Red blood cell distribution 0 to 50 PLT Platelet count 0 to 1490 x 10 uL within 4 0 CV 3 0 x 10 uL PCT Platelet crit 0 to 2 9 MPV Mean platelet volume 0 to 20 0 fL PDW Platelet distribution width 0 to 50 n panic value recount 0 to 599 x 10 uL MEK 6400 MEK 6410 only Standardization Analysis Method WBC ICSH1988 RBC ICSH1988 HGB NCCLS H15 A2 HCT NCCLS H7 A2 1 4 ICSH The assignment of values to fresh blood used for calibrating automated blood cell counters Clin Lab Haematol 10 203 212 1988 ICSH The assignment of values to fresh blood used for calibrating automated blood cell counters Clin Lab Haematol 10 203 212 1988 H15 A2 Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in Blood Second Edition Approved Standard 1994 H7 A2 Procedure for Determining Packed Cell Volume by the Microhematocrit Method Second Edition Approved Standard 1993 PLT Brecher amp Cronkite Method
161. o control the stepping motor s and valve s of each unit UT 7198 MEASURING Board This board which is attached to the MC 640V measuring unit sends the voltage between the electrodes liquid level sensor output voltages and HGB sensor output voltages to the UT 7192 AMP CONTROL board 3 10 Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION UT 7201 HGB AMP Board and UT 7202 HGB LED Board These boards which are attached to the MC 640V measuring unit sends the HGB optical density to the UT 7198 MEASURING board UT 7201 UT 7202 UT 7200 MIXED PUMP Board This board which is attached to the MD 640V combination syringe pump unit sends the position sensor output signal of the diluter piston to the UT 7193 POWER board Service Manual MEK 6400 6410 6420 3 11 3 BOARD UNIT DESCRIPTION UT 7205 PRINTER DRIVER Board This board which is attached to the WA 640VK printer unit receives the printer control signals from the UT 7192 AMP CONTROL board to drive the motor and thermal array head of the printer unit UT 7203 KEY Board This board which is attached to the PV 640VK front panel unit sends the status signals of the key switches on this board to the UT 7192 AMP CONTROL board 3 12 Service Manual MEK 6400 6410 6420 3 BOARD UNIT DESCRIPTION UT 7199 LIQUID SENSOR Board This board which is attached to the JQ 640V inlet outlet unit has two liquid sensors for detection of diluent detergent and he
162. o keep track of maintenance Daily Check reagent volume reagent expiration date recording paper and other consumables Check sampling nozzle open mode switches keys and outside surface of the analyzer Check reagent tube connection Check power cord and grounding lead connection Check external instrument connection printer PC bar code reader Check screen display and touch screen key function Calibrate touch screen Check date and time settings Check daily accuracy background noise measure hematology control Check measurement baths and sub baths Check pump tube Every 300 counts Check filters and filter packings Monthly or every 1 000 counts whichever comes first Replace filters Clean measurement baths and sub baths Clean rinse unit and check cap pierce nozzle Every four months or every 3 000 counts whichever comes first Check sampling nozzles Replace pump tube Every six months or as required Clean aperture caps Clean external electrodes on the measurement baths Check sensor monitor Check circuit Check X R values Check calibration coefficients Check priming function Check draining function Check cleaning function Check dispensing function Check external instrument function printer PC bar code reader Check that the 3 pin plug type power cord is used and the 3 pins and plug housing are not deformed Check that the resistance of the protective ground line
163. oint insert the tube into the fitting nut and turn the fitting nut clockwise to fasten it 00 AD Tube stopper Fitting nut Tube Board and Unit Location Refer to Board and Unit Location in Section 3 Service Manual MEK 6400 6410 6420 4 3 4 DISASSEMBLY AND ASSEMBLY Turning the Power Off Before turning the analyzer power off perform strong cleaning and drain the fluid from the instrument Cleaning and Draining the Fluid Pathway HOY 04 05 16 21 1 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen gt DOI HOY 09 05 19 50 2 Press the STRONG CLEAN key on the OPERATIONS screen PRIME DRAIN BATHS GO EL REMOVE CLOG 3 Replace the CLEANAC detergent with CLEANAC 3 detergent Refer to Connecting Tubes and Installing Reagents in Section 2 of the Operator s Manual OPERATIONS HOY 09 05 19 09 4 Press the YES key to perform strong cleaning The analyzer starts cleaning and the Strong cleaning message appears on the screen Replace CLEANAC with CLEANAC3 detergent After cleaning the instruction to replace the CLEANAC 3 detergent with CLEANAC detergent appears n 5 Ignore the message and press the OK key The screen returns to the READY Do strong cleaning screen YES NO 4 4 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 6 Remove the diluent tube from the ISO3 diluent inlet and the hemolysing reagent tube from the HEMO3
164. older is open This message appears only on the MEK 6400 analyzer only Reason Possible Cause Criteria Countermeasure Tube holder cannot Tube holder is open Close the tube holder be closed Holder hook does not move Replace the MS 641V cap pierce unit with a new one Replace the UT 7193 POWER board with a new one Replace the holder sensor with a new one Replace the UT 7193 POWER board with a new one The analyzer cannot detect if the holder is open or closed The holder sensor on the MS 641V cap pierce unit failure 2 24 Service Manual MEK 6400 6410 6420 A073 Tube in the holder Open mode cannot be selected because the sample tube is set in the tube holder This message appears only on the MEK 6400 analyzer only 2 TROUBLESHOOTING Reason Possible Cause Criteria Countermeasure Tube in the holder The sample tube is set in the tube holder Remove the sample tube and close the tube holder The analyzer cannot detect the tube The tube sensor on the MS 641V cap pierce unit failure Replace the tube sensor with a new one Replace the UT 7193 POWER board with a new one A091 Room temperature high This message appears when measurement is performed at temperatures over 30 C A092 Room temperature low This message appears when measurement is performed at temperatures below 15 C Reason Possible Cause Criteria Countermeasure Room temperatur
165. onnector CAUTION Be careful not to cut your finger with the edges of the chassis Sub bath motor cable connector 6 Pull out the measuring unit toward you Reattaching the Measuring Unit To reattach the measuring unit reverse the above procedure NOTE When reattaching the measuring unit place the measuring unit to the right as far as possible Service Manual MEK 6400 6410 6420 4 13 4 DISASSEMBLY AND ASSEMBLY Accessing the Connectors on the POWER Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the 2 screws which secure the POWER board to the chassis 4 Open the board to the right If it is difficult to open the board pull out the chassis marked A Reassembling the Board To assemble the board reverse the above procedure 4 14 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the Combination Syringe Pump Unit WARNING For your safety before disassembling the hematology analyzer wait 10 min
166. onnectors from the AMP CONTROL board Pull the cables to the POWER board side through the slots on the chassis AMP CONTROL board 4 30 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Remove the 2 screws which secure the POWER board to the chassis 6 Liftup the POWER board to unhook the POWER board from the 3 tabs on the chassis and pull out the board toward you Reattaching the POWER Board To reattach the POWER board reverse the above procedure Make sure that the hooks on the POWER board is hooked onto the tabs on the chassis refer to step 6 in the above procedure Service Manual MEK 6400 6410 6420 4 31 4 DISASSEMBLY AND ASSEMBLY Removing the MEASURING Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Do the procedure in Removing the Measuring Unit earlier in this section to remove the measuring unit from the chassis 3 Remove the 4 screws from the front panel of the measuring unit 4 Remove the screw from the rear panel of the measuring unit and remove the MC cover MC cover Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Remove the 3 cable connectors from the MEASURI
167. pe power cord is used and the 3 pins and plug housing are not deformed Check that the resistance of the protective ground line of the power cord is 0 1 Q Check that the earth leakage current is 0 5 mA or less under normal condition and 3 5 mA or less under each single fault condition Service Manual MEK 6400 6410 6420 6 11 6 MAINTENANCE Before Maintenance Procedure Before turning the analyzer power off for maintenance perform strong cleaning and drain fluid from the measurement baths and sub baths When background counts are out of specification or the clogging message frequently appears perform strong cleaning to clean the analyzer more thoroughly with CLEANAC 3 detergent containing sodium hypochlorite Strong Cleaning 1 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen HOY 04 05 16 21 QC CALIBRATION SETTINGS OPERATIONS _ OTHER PRIME DRAIN BATHS Replace CLEANAC with CLEANAC3 detergent Do strong cleaning YES GEM 6 12 Service Manual MEK 6400 6410 6420 6 MAINTENANCE 3 Replace the CLEANAC detergent with CLEANAC 3 detergent Refer to Connecting Tubes and Installing Reagents in Section 2 4 Press the YES key to perform strong cleaning The analyzer starts cleaning and the Strong cleaning message appears on the screen Press the NO key to cancel the procedure After cleaning the instruction to replace the C
168. pper manometer dirty ssssseeeen 2 20 A037 WBC lower manometer dirty isisisi isunei aieiaa 2 20 A041 RBC level 1 sesssssssssssseseseeeeeenneee nennen enn nennen nnns 2 21 A042 RBC leveli2 ern onere aa dada 2 21 A043 BBC level asta tee er uerit rte mert Ses aerate edulis dep 2 21 A044 RBC bubble Tercia raise 2 22 A045 RBC bubble 2 eoe dE iud e Resa a a dB re a gae Eu E HERR 2 22 A046 RBC bubble 3 nennen nennen 2 22 A047 RBC DUDDIOS cuido ideada data 2 22 A049 RBO Cl iria dla 2 22 A050 RBC sample error sssssssseeeeeeeeeen nennen nnns 2 23 A051 RBC noise Zorita 2 23 A052 PLENOISO eU iaa R 2 23 A053 PLT DOSO dana 2 23 A057 RBC upper manometer dirty sssssseeeeeene 2 23 A058 RBC lower manometer dirty ssssseeeeen 2 23 A061 HGB voltage lOW sasite anadenia nennen nennt nnns 2 24 A062 HGB voltage high ral 2 24 A063 NN 2 24 C 2 Service Manual MEK 6400 6410 6420 CONTENTS A072 Tube holder open sssesseeenn eene 2 24 A073 Tube in the Holder icu ii eet teer eres 2 25 A091 Room temperature high ccceeesecceeeeeeeneeeeeeeeeeeeseeeneeeeseeseeaneees 2 25 A092 Room temperature low cccecesseeccceeeeeeseeeeeeeeeesneeeeeeeeeneneeneaeees 2 25 A093 Internal temperature high ooooocccononoconicicononnnananccnnnnnnnnn nr 2 25 A094 Internal temperature low
169. ribes the maintenance that should be performed by qualified service personnel The Maintenance section in the operator s manual describes the maintenance that can be performed by the user NOTE If the instrument has a problem and there has been no periodic maintenance the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one The information in the operator s manual is primarily for the user However it is important for service personnel to thoroughly read the operator s manual and service manual before starting to troubleshoot service maintain or repair this instrument This is because service personnel needs to understand the operation of the instrument in order to effectively use the information in the service manual For simplicity the suffix C J K will be omitted in this manual There is no difference in operation and servicing among models with different suffixes unless otherwise specified Service Manual MEK 6400 6410 6420 1 GENERAL Service Policy CAUTION Be careful not to directly touch any place where blood is or may spread to Wear rubber gloves to protect yourself from infection before doing maintenance Nihon Kohden Corporation s basic policy for technical service is to replace faulty units printed circuit boards or parts We do not support component level repair of boards and units outside the factory NOTE When ordering par
170. rong cleaning If used for daily use HGB measurement may be affected e The Hemolynac 3 lysing reagent with cyan cannot be used There is no assay sheet for this reagent Service Manual MEK 6400 6410 6420 9 3 9 INSTALLATION Connecting Tubes NOTE Do not squeeze or bend the tubes Otherwise the analyzer may be damaged Follow the instructions on each package for handling the reagent Be careful not to let dust enter the lysing reagent diluent and detergent f Diluent Lysing reagent Detergent Diluent Tube NOTE Place the diluent container at the same level as the analyzer 1 Connect the diluent tube marked blue to the ISO3 inlet on the right side panel 18 L container cap 2 Pass the diluent tube through the 18 L container cap 18 L tube assy 2 Diluent tube marked blue 3 Connect the end of the diluent tube to the 18 L tube assy 2 4 Put the 18 L tube assy 2 into the diluent container and Diluent 18 L tighten the 18 L container cap 9 4 Service Manual MEK 6400 6410 6420 Detergent Tube 9 INSTALLATION NOTE Place the detergent containers at the same level as the analyzer Only use the specified detergent tubes for the detergent For performing STRONG CLEAN use CLEANAC 3 detergent For other purposes use CLEANAC detergent CLEANAC Detergent I 18 L container cap Derergent iube Cleanac tube assy marked green CLEANAC detergent 5 L 3
171. s Clean rinse unit and check cap pierce nozzle Only necessary on the MEK 6400 analyzer 6 8 Service Manual MEK 6400 6410 6420 6 MAINTENANCE Check Item OK No Every four months or 3 000 counts whichever comes first Check sampling nozzles Replace pump tube Every six months or as required Clean aperture caps Clean external electrodes on the measurement baths Enter the value HGB LED On 1 5 to 4 5 V HGB LED Off 0 5 V WBC 17 7 to 18 3 V RBC 17 7 to 18 3 V WBC manometer Upper gt 3 5 V WBC manometer Lower gt 3 5 V Without RBC manometer Upper 73 5 V reagent RBC manometer Lower gt 3 5 V Check sensor Diluent gt 3 5 V monitor Lysing reagent gt 3 5 V WBC manometer Upper 1 5 V WBC manometer Lower 1 5 V With RBC manometer Upper 1 5 V reagent RBC manometer Lower 1 5 V Diluent 1 5 V Lysing reagent 1 5 V HGB unit C MC unit C Power board C Enter the value WBC 7 6 to 8 4 x10 UL RBC 1 52 to 1 68 x10 uL MCV 85 to 115 fL PLT 152 to 168 x10 UL HGB ON 1 5 to 4 5 V HGB OFF 0 5 V WBC sensitivity WBC threshold RBC sensitivity RBC threshold PLT threshold Check circuit Service Manual MEK 6400 6410 6420 6 9 6 MAINTENANCE
172. s required 1 With the instrument power on press the Clean key on the front panel to perform cleaning Clean two or three times 2 The type of user must be Service to enter the SERVICE screen i On the MENU screen press the SETTINGS key USERS key HOY 04 05 16 21 HOY 04 05 14 40 MEASUREMENT IEASUREMEN OPERATION eum CALIBRATION Guy DATA OPERATIONS auum DATE amp TIME FAUTO CLEANING DISPLAY OUTPUT INITIALIZE ii Select Factory user and press the CHANGE key 111 Enter 4321 for the password using the numeric keys and press the Enter key Service Manual MEK 6400 6410 6420 3 5 ADJUSTMENT On the MENU screen press the OPERATIONS key PRIME key to fill the WBC and RBC measurement baths with diluent ENU MP HOY 04 05 16 21 GER PATOS Y f DATA SiS EIU NM MOV 04 05 16 21 Qa gt REMOVE CLOG STRONG CLEA cs 4 On the MENU screen press the OTHER key SERVICE key gt MONITOR key gt SENSOR MONITOR key to display the SENSOR HOY 04 05 16 21 OPER HISTORY Ya DAILY OUTPUT CIRCUIT CHEC MAINTENANCE BACKGROUND SERVICE D HGB WBC RBC lt Electrode gt lt Mano Reagent gt pper Lower WBC manometer 3 924 Y 4 334 RBC manometer 4 164 Z 4 254 MONITOR screen HOY 04 05 16 21 UH SE Cu K IHITIAL MENU OTHER MEHU eum
173. se in Section 6 The result appears on the screen after measurement Make sure that the values are less than or equal to the following values WBC 02 xl10 uL RBC 0 05 x10 uL HGB 0 1 g dL PLI 10 x10 UL When measured on the BACKGROUND screen Fail appears beside the parameter which is over the acceptable value Disregard the other parameter values because noise does not affect the other parameters If the values are not optimum check the following items press the yy Clean key on the front panel to clean the fluid path and recount the diluent If the values are still not optimum refer to Section 2 Troubleshooting Grounding Not sharing the AC outlet on the wall with other instrument The diluent is clean No bubbles in the diluent Remove clog from the aperture Do strong cleaning The external electrodes the screws on the measurement baths are securely fastened Filters are clean Service Manual MEK 6400 6410 6420 Calibrating Service Manual MEK 6400 6410 6420 9 INSTALLATION The aperture caps are clean The aperture caps are firmly attached The measurement baths and sub baths are clean For checking accuracy use a MEK 3D hematology control which has the same conditions as human blood For details on calibration refer to Section 7 Calibration of the Operator s Manual After calibration measure the hematology control again and check that the measured values
174. sensor does not detect no liquid at the start of RBC measurement or draining liquid from the manometer Reason Possible Cause Criteria Countermeasure Liquid is drained RBC manometer is dirty Perform strong cleaning but liquid cannot be RBC manometer upper sensor Adjust the sensor Refer to Adjusting the Upper and detected LED does adjustment error Lower Sensor Output Voltages of the Manometers in not turn off Section 5 The MC 640V measuring unit failure Replace the unit with a new one Liquid cannot be Pump tube is damaged Replace the pump tube with a new one drained The MP 640V pump unit failure Replace the unit with a new one The UT 7193 POWER board failure Replace the board with a new one The MC 640V measuring unit failure Replace the unit with a new one Electromagnetic valve failure Replace the UT 7192 AMP CONTROL board with a new one Replace the UT 7193 POWER board with a new one A042 RBC level 2 This message appears when the manometer upper sensor detects no liquid but the lower sensor does not detect no liquid at the start of RBC measurement or draining liquid from the manometer Reason Possible Cause Criteria Countermeasure Liquid is drained RBC manometer is dirty Perform strong cleaning but liquid cannot be RBC manometer lower sensor Adjust the sensor Refer to Adjusting the Upper and detected LED does adjustment error Lower
175. sensor for MS 640V LED for checking y axis position sensor for MS 640V LED for checking position sensor for MS 640V LED for checking upper position sensor for MS 641V D084 MS2LOW LED for checking lower position sensor for MS 641V D085 MS2OPEN LED for checking tube holder position sensor for MS 641V D086 MS2TUBE LED for checking tube sensor for MS 641V MEASURING Board a S EANIHON KOHDEN m 4 sem ngo skis enti Roa Q Q e D 1k NIHON KOHDEN me UT 7198 MEASURING BD UT 7198 M 6190 900706A 52 Po MEASURING BD TP011 5V TP012 ED TP013 15V TP014 EA TP015 15V TP016 36V TP017 E36 TP021 HGB ANA TP022 HGB TEMP TP023 MC TEMP D042 R UP D044 R LO D052 W UP D054 W LO 6190 900706A S1 oi 5 V DC terminal for digital circuit Ground terminal for digital circuit 15 V DC terminal for analog circuit Ground terminal for analog circuit 15 V DC terminal for analog circuit 36 V DC terminal Ground terminal for 36 V DC Test point for HGB sensor output Test point for HGB temperature output Test point for MC 640V temperature output LED for checking upper liquid sensor for RBC manometer LED for checking lower liquid sensor for RBC manometer LED for checking upper liquid sensor for WBC manometer LED for checking lower liquid sensor for WBC manometer Service Manual MEK 6400 6410 6420 7 5 7 T
176. st point for output waveform from the peak hold circuit at RBC side Test point for RBC threshold Test point for RBC digital pulse Test point for WBC pulses through the low pass filter Test point for WBC pulse analog waveform from the measuring unit Test point for WBC gate pulse Test point for output waveform from Robinson gate circuit for WBC Test point for WBC level Test point for voltage between the electrodes at WBC side Test point for WBC analog output waveform from the gain setting circuit Test point for output waveform from the peak hold circuit at WBC side Test point for WBC upper threshold Test point for WBC digital pulses which exceed the upper threshold Test point for WBC lower threshold Test point for WBC digital pulses which exceed the lower threshold Test points for HGB voltage from the HGB AMP board Service Manual MEK 6400 6410 6420 1 3 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD POWER Board NIHON KOHDEN 1 POWER BD UT 7193 6190 900528B S al TP011 36V TP012 15V TP013 EA TP014 15V TP015 Vu TP016 M15V TP017 EM15 TP018 Eu TP019 5V TP0110 3 3V TP0111 ED TP0112 VCC TP0113 Vp TP0114 EP TP021 POWER SW031 POWER SW032 D016 Vcc D017 15V D018 36V D019 Vu D0110 M12V D011
177. t 5 iret oett ss 4 13 Accessing the Connectors on the POWER Board sssssssssssssseeeseeeeeeeen a uenia narran nnne nennen nnns 4 14 EE NN eene seen nennen nenne hne enne nennen nennen nnns 4 14 Removing the Combination Syringe Pump Unit ssssessssssseseesseseeeneee eene 4 15 Reattaching the Combination Syringe Pump Unit oooooccccnnnocinonoccncnonannnanan conan non cnnnnnnnannn co nann cnn nana na nannn cane 4 16 REMOVING The Pump Units sitial idiotas 4 17 Reattaching the PUMP Unit escocia EEN 4 18 Removing the Sampler Unit nennen nennen nennen enne n sen nr aran nnns enne n nnn nnn nnne nen 4 19 Reattaching the Sampler Unit cete iii da cx te as 4 22 Removing the Cap Pierce Unit or Rinse Unit nennen ran cnn nnns eterne nnne anaiai 4 23 Reattaching the Cap Pierce Unit or Rinse Unit essssssssssseeeeeeeeneeeen mener 4 24 Removing the Inlet Outlet Unit usina ai A ec edd ete 4 25 Reattaching the InleU Outlet nil ottenere coeno eaaa edv ves Eo rub d 4 26 REMOVING the Valve Unitaria eter betur aia 4 26 Reattaching the Valve Unii m 4 27 Removing the AMP CONTROL Board ccoooocccccooccnononcncnononcncononononnnncnnnnnn canon nn ran n nr entres sen rns sed t rines ennt rsen nnne nnn nen 4 28 Reattaching the AMP CONTROL Boat sissien sasra nennen nennen nnne nennen EE nnne nnne nennen 4 29 Removing the POWER BOard e
178. t the background noise is reduced If the data of the background check is still not optimum replace the diluent with diluent from a new sealed container NOTE Once the background noise is reduced and when the instrument is used every day the instrument is not severely contaminated However contamination builds up in the instrument and cannot be easily removed if the instrument is not cleaned periodically Periodic maintenance is important for keeping the instrument in optimum condition When not using the instrument for a long time clean the fluid path with diluent Refer to Storing and Transporting the Analyzer in Section 9 of the Operator s Manual Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING Background Noise Problems Problem Possible Cause Criteria Countermeasure High background Thedilient or dient container fs dirty Clean the diluent container or replace the diluent noise for all with new one parameters The fluid path inside the hematology analyzer Press the Clean key on the front panel to perform is dirty cleaning Perform strong cleaning Noise interference through AC source or Connect the hematology analyzer power cord to other instruments nearby another AC outlet If possible use an independent AC outlet only for the hematology analyzer Perform equipotential grounding and remove the other instruments if they are the cause
179. t the waste tube marked red to the waste outlet on the right side panel Pass the waste tube through the MEK cap Connect the waste tube to the waste tube assy Insert the waste tube assy into the 2 L container waste container and tighten the MEK cap 9 6 Service Manual MEK 6400 6410 6420 Turning Power On Off Turning On the Power 9 INSTALLATION 1 Confirm that the analyzer and optional external instrument power cords are connected to grounded power outlets Main power switch the analyzer Main power lamp 2 Setthe printer power switch ON 3 Press the Main power switch on the rear panel to ON The Main power lamp on the front panel lights Always leave the main power ON except for storage and transportation of 4 Press the Power key on the front panel ON The Power lamp lights and Wd o coeno n 4 amp 8 oo NE Power lamp Power key the screen illuminates within 15 to 30 seconds Cleaning of the fluid path priming and circuit self check are automatically performed HOY 04 05 15 11 Detergent Lysing reagent Diluent Priming Circuit check Operation check Maintenance check Remaining time minutes 1 When there is an error the Fail appears on the screen Press the DETAILS key to display details on the alarm HOY 10 05 14 49
180. tch Reset key switch Clean key switch Eject key switch arora 7 7 7 TEST POINT VARIABLE RESISTOR LED AND SWITCH ON BOARD PRINTER DRIVER Board 6190 900653 8 JAPAN JE 04 05 MIL re oun numerus 19 0o rus Lis ost Ou 6023 EE un io E At Tai FLOIS Pals Lp FLO Epa S amp yai o me m CD24 a 210126 TIT 20112 E zoni s 20110 mao NOR O TP011 5VP 5 V DC terminal for printer TP012 EDP Ground terminal for printer TP013 5V 5 V DC terminal for digital circuit TP014 ED Ground terminal for digital circuit TP015 HDVp Supply voltage for thermal array head of printer TP031 REF Test point for reference voltage 7 8 Service Manual MEK 6400 6410 6420 Section 8 Socket Pin Assignment ZK 820V Bar Code Reader Sockets A Aa 8 2 Serial Port 1 Serial Port2 icono iaa shade ada leida as 8 2 Printer SOC o A 8 2 A oos mU 8 2 Service Manual MEK 6400 6410 6420 8 1 8 SOCKET PIN ASSIGNMENT CAUTION Connect only the specified instruments to the analyzer and follow the specified procedure Failure to follow this instruction may result in electrical shock or injury to the operator and cause fire or instrument malfunction ZK 820V Bar Code Reader Socket D sub 9 pin ZK 820V Pin No Signal Pin No Signal Pin No Signal FG 4 To pin 6 7 CTSO 92 2 TXDO 5 SG 8 RTSO LOJ 3 RXDO 6 To pin 4 9 VC
181. tes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the sampler unit Refer to Removing the Sampler Unit earlier in this section 4 Remove the cable connector from the POWER board red and white cable Remove the cable binder from the cable To access the POWER board refer to Accessing the Connectors on the POWER Board earlier in this section Cable connector 4 26 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY 5 Remove the 2 screws which secure the valve unit to the chassis 6 Tilt the valve unit toward you and remove the 9 tube joints from the valve unit Right side panel P s d RE Chassis POWER board 7 Pullout the valve unit toward you Reattaching the Valve Unit To reattach the valve unit reverse the above procedure Service Manual MEK 6400 6410 6420 4 27 4 DISASSEMBLY AND ASSEMBLY Removing the AMP CONTROL Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Re
182. trong cleaning unit failure Clean the WBC aperture WBC aperture is damaged Replace the WBC aperture with a new one The screw securing the measurement bath Tighten the screw is loose The MC 640V measuring unit and chassis Remove the cause are not insulated there may be leakage The MC 640V measuring unit failure Replace the unit with a new one A036 WBC upper manometer dirty The voltage of the upper liquid sensor on the manometer is 2 25 to 2 75 V Reason Possible Cause Criteria Countermeasure Manometer is dirty The WBC manometer is dirty Perform strong cleaning Sensor voltage adjustment error error WBC manometer upper sensor adjustment Adjust the WBC manometer upper sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in Section 5 A037 WBC lower manometer dirty The voltage of the lower liquid sensor on the manometer is 2 25 to 2 75V Reason Possible Cause Criteria Countermeasure Manometer is dirty The WBC manometer is dirty Perform strong cleaning Sensor voltage adjustment error error WBC manometer lower sensor adjustment Adjust the WBC manometer lower sensor Refer to Adjusting the Upper and Lower Sensor Output Voltages of the Manometers in Section 5 2 20 Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING A041 RBC level 1 This message appears when the manometer upper
183. ts or accessories from your nearest Nihon Kohden Corporation s representative please quote the NK code number and part name which is listed in this service manual and the name or model of the unit in which the required part is located This will help us to promptly attend to your needs Always use parts and accessories recommended or supplied by Nihon Kohden Corporation to assure maximum performance from your instrument Service Manual MEK 6400 6410 6420 1 3 1 GENERAL Specifications Measured Parameters Ranges and Reproducibility to Specimen from Venous Blood Specifications except WBC population were determined using hematology control blood MEK 3DN counted 10 times consecutively Measured Parameters Measuring Range Reproducibility to Specimen from Venous Blood CV Coefficient of Variation WBC White blood cell count MEK 6400 6410 0 to 59 x 10 uL MEK 6420 0 to 99 x 10 uL within 2 0 CV 4 0 to 9 0 x 10 uL within 5 0 CV WBC 4 0 to 9 0 x 10 uL 07 0 LY Lymphocyte percent 0 to 99 9 LY 20 to 45 0 within 12 0 CV WBC 4 0 to 9 0 x MO Monocyte percent 0 to 99 9 10 UL MO 2 to 10 GR 9 Granulocyte percent 0 to 99 9 within 5 0 CV WBC 4 0 to 9 0 x 10 uL GR 40 to 70 LY Lymphocyte count MEK 6400 6410 0 to 59 x 10 UL MEK 6420 0 to 99 x 10 uL MO Monocyte count MEK 6400 6410 0 to 59 x 10 uL MEK 6420 0 to 99 x 10 uL GR Gra
184. ttach the right side cover and fasten it with the three screws on the rear panel and one screw on the right side panel 10 Press the Power key to turn on the power The analyzer starts priming the fluid pathway 11 If the pump tube was replaced the pump tube counter will have to be reset To reset the counter press the RESET key for Pump tube on the OPER HISTORY screen to reset the counts to zero 12 Fill in the Maintenance Check Sheet Service Manual MEK 6400 6410 6420 6 MAINTENANCE Every Six Months As Required Maintenance Procedures Removing a Clog from the Aperture When the CLOG alarm occurs remove the clog by the following procedure 1 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen HOY 04 05 16 21 CALIBRATION SETTINGS pro c OPERATIONS 2 Press the REMOVE CLOG key on the OPERATIONS screen The confirmation message appears HOY 09 05 18 50 HOY 09 05 18 55 PRIME DRAIN BATHS A Eni NR GATA ET a Remove clog 3 Press the YES key to remove the clog from the aperture The analyzer starts removing the clog and the Removing clog message appears on the screen Press the NO key to cancel the procedure After removing the clog the screen returns to the OPERATIONS screen Service Manual MEK 6400 641 0 6420 6 35 6 MAINTENANCE Cleaning Aperture Caps Materials Required Powder free gloves lab coat safety glass
185. ue listed in the assay sheet which is attached to the hematology control Write down the values of the lt Data gt field and lt Cal gt field Repeat the procedure in open mode then in pre dilution mode NOTE Other calibration method may be used in some laboratories Before changing calibration coefficients check with the laboratories on the calibration method This function fills the fluid path inside the analyzer with diluent Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen Press the PRIME key The Prime confirmation message appears Press the YES key to prime The analyzer automatically checks the reagent and starts priming Press the NO key to cancel the procedure The screen returns to the OPERATIONS screen During priming the screen shows the Priming message After priming is completed the screen returns to the READY screen Refer to Before Maintenance Procedure earlier in this section Press the yiy Clean key on the front panel to check the cleaning function After cleaning the screen returns to the READY screen For checking the strong cleaning function refer to Before Maintenance Procedure earlier in this section Service Manual MEK 6400 6410 6420 6 MAINTENANCE Checking the Dispensing Function 1 On MEK 6400 On the READY screen check that Open is selected for lt Sampling mode gt and select Pre dil for lt Dilute mode gt
186. ue npe ice 2 34 CONT MEASURE SCree m 2 35 SETTING MENU Sree cetreria tas 2 35 ADV SETTINGS Screen cocooncccccnncncccnnnncnnonnncnnnnnnnnnnnnn nn rnn cnn nennen nnne nnns 2 35 REAGENT FACTOR Soreen 1 neces ea 2 36 USER MAINTENANCE Screen iiras ideata eaaa aen aaiae 2 36 PANIC VALUE Screen for MEK 6400 and MEK 6410 2 37 FLAG SETTINGS Screen iue iia iii cado aad 2 37 BAR CODE PORT Screen ccccccnocccccooccnnnnnncnnnnnncnconnncnnannnconannncn nana na rannncinnns 2 37 UNIT CAL Screen sect aeiia ES 2 38 MONITOR Screen mE 2 38 X1O CV SC Mica aleta 2 39 INITIAL MENU SOre6n incio 2 40 OTHER MENUS CrOeN 2 40 PRINT SETTINGS Scre amp n ntt eene dentes itle riada 2 40 Section 3 Board Unit Description eese eere 3 1 Board and Unit LOCaLORn scarico redo dece deutet edo Dv veu aa pu Dean Du e Atte d Right Side View oooooconccccnoocccnconcncncnnnncnnannncnnannn cocoa cnn nan nc n nennen rennes nnne n enirn nnns 3 3 Service Manual MEK 6400 6410 6420 C 3 CONTENTS Section 4 C 4 Left Side View IA 3 3 uto A E ies 3 4 TOP Mi Wii aa 3 4 MC 640V Measuring Unitrin rere eren aioi hn tache sa e E Ea a ds 3 5 MD 640V Combination Syringe Pump Unit 3 5 MP 640V Pump Unit eene enne nnne nennen nennen rennes 3 6 IV 641V ErontParielDI nicer tot Doer a 3 6 JQ 640V Inlet Outlet Unit cintia tede eec i a ev ELLE eges 3 7 JQ
187. unit cannot be initialized Reason Possible Cause Criteria Countermeasure The sampler unit does not perform the initialization Sampling nozzles do not move Replace the sampling nozzle with a new one The cap on the rinse unit or cap pierce unit for cap piercing catches the nozzle Replace the cap with a new one Connector s on the sampler unit is disconnected Reconnect the connector Cable s on the sampler unit is damaged Replace the cable with a new one Sampler initialize position sensor failure Replace the sampler initialize position sensor with a new one Sampler separating sensor failure Replace the sensor with a new one Motor driver to the UT 7193 POWER board failure Replace the board with a new one Sampler motor failure Replace the MS 640V sampler unit with a new one E041 SUB BATH INITIALIZE ERROR The sub baths of the MC 640V measuring unit cannot be initialized Reason Possible Cause Criteria Countermeasure Sub baths do not The connector of the sub bath sensoris Reconnect the connector of the sub bath sensor rotate disconnected Sub bath is not attached properly Reattach the sub bath Cap is not attached properly Reattach the cap Cable s on the measuring unit is damaged Replace the MC 640V measuring unit with a new one Sub bath motor failure Replace the MC 640V measuring unit with a new one
188. upper sensor ON Reason Possible Cause Criteria Countermeasure Air bubbles in the RBC manometer is dirty Perform strong cleaning manometer RBC aperture is not attached properly Reattach the RBC aperture The MC 640V measuring unit failure Replace the unit with a new one A047 RBC bubble 4 This message appears when the RBC measurement or aspirating sample time is too short from lower sensor ON to upper sensor ON Reason Possible Cause Criteria Countermeasure The aperture is too RBC aperture is damaged Replace the RBC aperture with a new one large Incorrect aperture 100 um Use an appropriate aperture 80 um Air bubbles in the No liquid Perform priming manometer The MC 640V measuring unit failure Replace the unit with a new one A049 RBC clog This message appears when the manometer lower sensor detects the liquid but the upper sensor does not detect the liquid during RBC measurement or aspirating sample Reason Possible Cause Criteria Countermeasure Measuring pressure can be produced but there is clog RBC aperture is clogged Clean or replace the RBC aperture with a new one The MC 640V measuring unit failure Replace the unit with a new one Cannot produce measuring pressure The pump tube is damaged Replace the pump tube with a new one The MC 640V measuring unit failure Replace the unit with a new one Measuring pressure
189. ure the sample immediately Leukemia An increase in WBC in leukemia patient causes increase in RBC Agglutinated RBC Agglutinated RBC may decrease RBC count This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Cold agglutinins IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and increase MCV Hemolysis When RBC is hemolyzed RBC is decreased Turbidity of the blood sample Any physiologic and or therapeutic factors may increase HGB In such a case determine the cause of turbidity and follow the appropriate method below 1 Increased WBC An extreme increase in WBC causes excessive light scatter In these cases measure manually Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer 2 Increased lipids The blood sample may be milky when there is excessive lipids This may occur with hyperlipidemia hyperproteinemia and hyperbilirubinemia Accurate HGB measurement can be achieved by manual methods and a plasma blank 3 Increased turbidity When RBC are resistant to lysing turbidity may increase causing increase in HGB Observe if MCH and MCHC values are abnormal HGB result affects MCH and MCHC result 4 Fetal bloods The mixing of fetal and maternal blood may increase HGB value Service Manual MEK 6400 6410 6420 1 13 1 GENERAL HCT MCV MCH MCHC PLT MPV 5 High WBC levels
190. utes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Loosen the 2 screws A and B which secure the combination syringe pump unit to the chassis Tube joint 1 Screw B 4 Remove the 5 tube joints from the combination syringe pump unit Screw A Tube joint 4 Tube joint 5 Screw B Tube joint 2 Tube joint 3 Service Manual MEK 6400 6410 6420 4 15 4 DISASSEMBLY AND ASSEMBLY 5 Remove the cable connector from the MIXED PUMP board and stepping motor Cable connected to stepping motor Tube joint 5 MIXED PUMP board Cable connected to MIXED PUMP board 6 Pull out the combination syringe pump unit by tilting it toward you Be careful not to damage any tube Reattaching the Combination Syringe Pump Unit To reattach the combination syringe pump unit reverse the above procedure 4 16 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the Pump Unit WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument
191. wer supply 3 Effect of direct or indirect electrostatic discharge Make sure all users and patients in contact with the equipment and or system are free from direct or indirect electrostatic energy before using it A humid room can help lessen this problem 4 Electromagnetic interference with any radio wave receiver such as radio or television If the equipment and or system interferes with any radio wave receiver locate the equipment and or system as far as possible from the radio wave receiver If the above suggested remedial actions do not solve the problem consult your Nihon Kohden Corporation subsidiary or distributor for additional suggestions This equipment complies with International Standard EN55011 1999 Group 1 Class B Class B EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes The CE mark is a protected conformity mark of the European Community The products herewith comply with the requirements of the IVD Directive 98 79 EEC Service Manual MEK 6400 6410 6420 iii NOTE about Waste Electrical and Electronic Equipment WEEE directive 2002 96 EEC For the member states of the European Union only The purpose of WEEE directive 2002 96 EEC is as a first priority the prevention of waste electrical and electronic equipment WEEE and in addition the reuse recycling an
192. with a new one Poor HCT or MCV Reproducibility The following may be the main reasons for poor HCT or MCV reproducibility Measuring unit is dirty Measurement settings are inappropriate Hematology analyzer failure Measuring unit is dirty Possible Cause Criteria Countermeasure Measurement baths and sub baths are dirty Clean the measurement baths and sub baths Aperture is dirty Clean the aperture Inappropriate measurement settings Possible Cause Criteria Countermeasure The sensitivity and threshold settings are not appropriate Check that the sensitivity and threshold settings are set as below RBC SENSITIVITY 5 RBC THRESHOLD AUTO Hematology analyzer failure Possible Cause Criteria Countermeasure The aperture is damaged Replace the aperture with a new one Circuit malfunction RBC is outside 1 60 5 when circuit check is performed by pressing MENU key OTHER key gt CIRCUIT CHECK key Replace the MC 640V measuring unit with a new one Replace the UT 7198 MEASURING board with a new one Replace the UT 7192 AMP CONTROL board with a new one RBC sample error Replace the MD 640V combination syringe pump unit with a new one In closed mode replace the MS 641V cap pierce unit with a new one In open mode replace the MS 640V sampler unit with a new one Service Manual MEK 6400 6410 642
193. ws from the KEY board Reattaching the KEY Board To reattach the KEY board reverse the above procedure 4 36 Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Removing the LIQUID SENSOR Board WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off earlier in this section to turn off the instrument 2 Remove the right side cover top cover and rear cover Refer to Removing the Right Side Cover Top Cover and Rear Cover earlier in this section 3 Remove the inlet outlet unit Refer to Removing the Inlet Outlet Unit earlier in this section 4 Remove the cable connector from the LIQUID SENSOR board 5 Remove the 2 tube joints 6 Remove the 2 screws which secure the LIQUID SENSOR board to the inlet outlet unit Screw Cable connector Screw Tube joints Reattaching the LIQUID SENSOR Board To reattach the LIQUID SENSOR board reverse the above procedure Service Manual MEK 6400 6410 6420 4 37 4 DISASSEMBLY AND ASSEMBLY Removing the LCD WARNING For your safety before disassembling the hematology analyzer wait 10 minutes after turning off the main power switch Remove all cables and cords connected to the instrument 1 Do the procedure in Turning the Power Off
194. x socket driver Tweezers Service Manual MEK 6400 6410 6420 4 DISASSEMBLY AND ASSEMBLY Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the Instrument CAUTION Valve Joint When connecting the valve joint to the electromagnetic valve turn 93 3 the valve joint clockwise using moderate force until the valve joint comes to a stop Do not use extreme force to tighten the valve joint further because this will damage the tip of the valve joint If the valve joint is loosely connected it will leak NOTE Black screw Black screws are used to fasten the individual units to the chassis of the instrument to enable the quick removal and replacement of these units However to fasten the pump unit to the chassis normal Screws are used Spring type tube joint Spring type tube joints are used in the instrument to prevent over tightening of the joints and to prevent loosening of the joints after the joints are tightened There are 2 types of spring tube joints white inlet side and black outlet side Each tube joint and its corresponding port in the instrument are marked with the same color or number to ensure matching No spring type tube joint The no spring type tube joint consists of an O ring tube stopper and fitting nut To disconnect the tube from this joint turn the fitting nut counterclockwise to loosen the joint and pull the tube toward you To reconnect the tube to this j
195. y analyzer according to the following check procedure flowchart Are the following conditions acceptable Reagents Temperature Blood sample Does the screen appear with no error messages such as the following when the power is turned on No diluent No detergent No lysing reagent e XXX initialize error XXX priming error Check the specified parameters after the background noise measurement Are the WBC RBC HGB and PLT data acceptable s there no alarm message Check the following after the MEK 3D hematology control measurement s there no alarm message Are the following parameter reproducibility acceptable RBC HGB WBC PLT HCT and MCV Are all the obtained parameters within the acceptable range on the assay sheet 24 Refer to the Operating Condition and Blood Sample Checks section Refer to the Solving Problems from Alarm Messages and Solving Problems from System Error Messages sections Refer to the Checking Background Noise section Background Noise Problems table and Solving Problems from Alarm Messages section Refer to the Solving Problems from Alarm Messages and Checking Accuracy sections Service Manual MEK 6400 6410 6420 2 TROUBLESHOOTING Operating Condition and Blood Sample Checks Operating Condition Check Measurement requires the following operating conditions Operating temperature 15 to
196. yzer is about 7 minutes to 52 seconds per month Measuring Background Noise 6 18 Measure background noise in closed mode and open mode Closed mode is only for MEK 6400 1 Select Closed or Open for Sampling mode and press the MENU key on the READY screen HOY 04 05 15 26 0001 Sanple type Sampling mode Y Open Y Closed MENU 2 Press the OTHER key on the MENU screen HOY 04 05 16 21 CALIBRATION SETTINGS DATA OPERATIONS Service Manual MEK 6400 6410 6420 6 MAINTENANCE 3 Press the BACKGROUND key on the OTHER screen U HOY 04 05 16 40 oz HISTORY BM DAILY OUTPUT CIRCUIT CHEG MAINTENANCE Gib uo BACKGROUND Y The Measure background noise message appears on the screen 4 Press the YES key to measure background noise The result is displayed after the measurement is complete HOY 04 05 17 30 Make sure that the values are less than or equal to the following values WBC 0 2 x10 uL RBC 0 05 x10 uL HGB 0 1 g dL PLT 10 x10 uL Fail appears beside the parameter which is over the acceptable value Disregard the other parameter values because noise does not affect the other parameters Service Manual MEK 6400 6410 6420 6 19 6 MAINTENANCE Tf the values are greater than the values listed above check the following items press the y Clean key on the front panel to clean the flu

Nihon Kohden Celltak MEK-6400

Contents

Download Pdf Manuals

Related Search

Related Contents