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User and Service Manual

1. ij e A 3 970mm 1035mm 352mm 38 2 40 7 13 9 E E M E 1270mm 1340mm 458mm 5 ATT 1 50 0 52 7 18 0 D oe ae 5 1570mm 1645mm 520mm T M ERA 2 61 8 64 7 20 5 PMOL LY 3 1870 1950 975mm S 3 73 6 76 7 384 250mm 9 8 ei 2 SS 7 2 UPS T N z wae ES rd x eo X COM ETC AS AAA Aj PR 14 8 A A 15 2 16 5 s E em Beo 4 17 S 10 5 E E 6 12 9 13 es 550mm 21 7 A 732mm 28 8 800mm 31 5 1 Sensor Box 7 Exhaust Collar 12 Electrical Outlet 2 RS232 Port Optional Retrofit Kit Provision 3 Power Inlet 8 Electrical Panel 13 Armrest 4 Sentinel Gold 9 Fluorescent Lamp s 14 Exhaust ULPA Filter Controller 10 IV Bar Retrofit Kit 15 Blower 5 Sash Window Provision 16 Downflow ULPA Filter 6 Single Piece Work 11 Plugged Service 17 UV Lamp Tray Fixture Provision Class Biological Safety Cabinets Ecco 9 2 2 General Specification _ _ cs 2 5 AC2 5S__ AC2 6S_ External Dimensions 1950 x 800 x 1400 mm
2. 5 5 COLOR AWG 1 Black 14 2 White 14 3 Green 14 4 Red 14 5 Blue 14 6 GN 14 7 Red 14 8 Red 14 9 Red 14 10 Red 14 11 Red 14 12 Red 14 13 Blue 14 14 Blue 14 15 Blue 14 16 Blue 14 17 Blue 14 18 Red 14 19 Red 16 20 Red 16 21 Red 16 22 Red 16 23 Blue 16 24 Red 16 25 Blue 16 26 Red 16 27 G Y 16 28 Red 16 29 Blue 16 30 G Y 16 31 Red 16 32 Red 18 33 Blue 18 34 Red 18 35 Blue 18 36 Red 18 37 Black 18 38 Black 18 39 Red 18 40 Red 18 41 Brown 18 42 Brown 18 43 Yellow 18 44 Yellow 18 45 Blue 18 46 Red 18 47 Red 18 48 Black 18 49 Yellow 18 90 Black 14 51 Black 18 52 Blue 18 53 Brown 16 54 Blue 16 55 Blue 14 56 Red 14 57 G Y 14 58 G Y 14 59 G Y 14 60 G Y 14 61 G Y 16 62 Red 16 63 Blue 16 64 Brown 16 NO COLOR AWG 101 Black 18 102 White 18 103 Green 18 Fluorescent Ballast NO COLOR AWG 107 Black 18 108 White 18 109 Red 18 110 Red 18 111 Blue 18 112 Blue 18 113 Yellow 18 114 Yellow 18 115 Yellow 18 116 Yellow 18 117 Red 18 118 Red 18 119 Blue 18 120 Blue 18 121 Yellow 18 122 Yellow 18 123 Yellow 18 124 Yellow 18 UV Ballast NO COLOR AWG 1
3. To Reset B H M 1 INNEN Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose RESET B H M Press SET button to confirm The display will Show READ MANUAL and PRESS SET Press SET button to confirm The display will Show CONFIRM Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display 2 2 4 6 Reset UV H M This option is used to reset the UV lamp hour meter The UV lamp hour meter indicates how long the UV lamp has been in operation Maximum counter is set at 2000 hours 10096 The counter value can be checked while in the diagnostic mode Please reset the UV lamp hour meter after each UV lamp replacement MENU gt ADMIN SETTINGS RESET UV H M E co Airstream Reset UV H M 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS
4. MAINTENANCE MODE AIRFLOW CALIB FIELD CALIBRATION RESET CALIBRATION NEW ADMIN PIN Default 0009 NEW FAN PIN Default 0000 AIRFLOW MONITOR ENABLE DISABLE FILTER LIFE DISP ENABLE DISABLE ADMIN SETTINGS SET LANGUAGE ENGLISH FRANGAISE ESPANOL 2 2 1 Settings Users may use the settings menu function to customize the operation of the BSC to meet specific application RESET B H M RESET UV H M RESET F H M RESET DEFAULT AIRFLOW OUT TEMP ENABLE DISABLE requirements The settings menu can be entered using both FAN PIN and ADMIN PIN E co Airstream Lt 2 2 1 1 Set Clock Time Users can set the time by increasing decreasing the hour and minute values The correct time will be maintained even after the unit is turned off MENU SETTINGS p SET TIME p HH MM To Set Time 1 Press MENU button to enter the menu display if the BSC is secured by FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm T
5. AC2 D and AC2 G blower plenum exhaust blower attached to frame AC2 E and AC2 S blower plenum To replace the blower follow the filter replacement steps until the blower plenum can be removed from the cabinet then follow these steps 1 Take out the blower housing 2 Unscrew the bottom plate to separate the top and the bottom section 3 Unscrew the impeller from the bottom plate The number of blower s may vary between each cabinet size and series 5 2 3 Fluorescent Lamp s Replacement Procedure 1 Disconnect the electrical connections 2 Unfasten the two screws from the bottom of the blue panel and then lift up the blue panel Caution The front panel is equipped with a gas spring which will open the front panel automatically 3 Thefluorescent lamp s is located behind the blue panel Disconnect the old one s and replace with the new one s 5 2 4 UV Lamp Replacement Procedure 1 Disconnect the electrical connections 2 Take out the old UV lamp by rotating it 90 counter clockwise and pull downward 3 Install the new UV lamp by pushing it upward and rotating it 90 clockwise to secure the lamp in its socket Class Biological Safety Cabinets Ecco 5 2 5 Airflow Sensor Replacement Procedure Airflow Sensor Cable ties Temperature Sensor In AC2 E and AC2 S series the airflow sensor is located on top of the cabinet The sensor can easily be accessed in these cabinets However in AC2 D and AC
6. distance between the cabinet aperture and any The distance between the side panel and any doorway is at least 1 5 m 5 doorway is at least 1 0 m 37 30 1 SS A nn YY 3 m 10 Allow at least 3m 10 between the aperture to the Any large obstruction projecting beyond the plane of aperture of an opposing cabinet fume cupboard etc the front aperture should not be within 30 cm 1 of the sides of the cabinet 1 5 m 5 You should not position the cabinet in a location The distance between cabinet aperture and the front where there is an obstruction that affect airflow of a bench opposite should be at least 1 5 m 5 within 2 m 7 of the front aperture 1 3 Avoid positioning bench at right angles the A projecting bench will help minimize traffic in front cabinet Any person working at the bench is likely to of the cabinet as long as the front ofthe bench is disturb airflow close to the cabinet situated at least 1 m 3 from the side ofthe cabinet E co Airstream 4 1 1 2 Relocating Cabinet Normally BSCs rarely moved once they their ideal positions but if the need arise to relocate or even repackage the unit then here is some considerations e Before moving the BSC remember to decontaminate the cabinet e Before moving the BSC remember to secure all moving parts ex sash e BSCis heavy be caref
7. Class Biological Safety Cabinets ECCO 4 European Union Directive WEEE and RoHS The European Union has issued two directives e Directive 2002 96 EC on Waste Electrical and Electronic Equipment WEEE This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Esco sells products through distributors throughout Europe Contact your local Esco distributor for recycling disposal e Directive 2002 95 EC on Restriction on the use of Hazardous Substances RoHS With respect to the directive on RoHS please note that this hood falls under category 8 medical devices and category 9 monitoring and control instruments and is therefore exempted from requirement to comply with the provisions of this directive ECZO Airstream vii Declaration of Conformation In accordance to EN ISO IEC 17050 1 2004 We Esco Micro Pte Ltd of 21 Changi South Street 1 Singapore 486777 Tel 65 6542 0833 Fax 65 6542 6920 declare on our sole responsibility that the product Category Class Biological Safety Cabinet Brand Airstream Model AC2 4D1 AC2 6D1 AC2 2E1 AC2 3E1 AC2 4E1 AC2 5E1 AC2 6E1 AC2 4G1 AC2 6G1 2 351 2 451 2 551 2 651 accordance with the following directives 2006 95 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromagn
8. cc 9 zu Wr ac ac 52 2 Oz E 2 au 9 S 5 As seen in the chart your cabinet s internal airflow velocity is relatively low when compared to the airflow disturbances potentially caused by the opening of a door a person walking by or a direct exposure to an air conditioning outlet These external airflow disturbances can affect the proper laminar flow of the BSC and impaired the protection offered by the cabinet When installing the BSC it should be located as far away as possible from sources of airflow disturbance and in an orientation which optimally shields the cabinet s airflow from all external airflow disturbances The following requirements should be taken into account Poor siting of a cabinet can adversely affect performance A specialist engineer should be consulted on correct positioning of the cabinet prior to installation Cabinets should never be sited in line with a doorway an open able window or adjacent to a thoroughfare Care should be taken to ensure that possible disturbances to airflow such as room air diffusers fans extractors vents etc are taken into account and any risk of disturbance noted and mitigated before installation Room air supply diffusers should not be within 1 5 meter 5 of the front aperture If there are large numbers of cabinets in a laboratory this recommendation may be difficult to comply with but where diffusers have to be placed in close proximity to a sa
9. Eccc m Airstream Thank you for purchasing this Esco Biological Safety Cabinet Please read this manual thoroughly to familiarize yourself with the many unique features and exciting innovations we have built into your new equipment Esco provides many other resources at our website www escoglobal com to complement this manual and help you enjoy many years of productive and safe use of your Esco products User and Service Manual For Technical Service contact North America Esco Technologies Inc 2940 Turnpike Drive Units 1516 Hatboro 19040 USA Toll Free USA and Canada 888375 ESCO Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com Airstream Rest of World Esco Micro Pte Ltd 21 ChangiSouth Street 1 Singapore 486 777 Class Tel 65 6542 0833 Fax 65 6542 6920 www escoglobal com mail escoglobal com Bio ogica Safety Ca bi net Esco AC2 E S D G User amp Service Manual Version A Released November 2010 Copyright Information Copyright 2010 Esco Micro Pte Ltd All rights reserved The information contained in this manual and the accompanying product is copyrighted and all rights are reserved by Esco Esco reserves the right to make periodic minor design changes without obligation to notify any person or entity of such change Sentinel and Airstreamo are registered trademarks of Esco Material in this manual is provided for informational pu
10. WxDxH 40 7 x 31 5 x 55 1 52 7 x 31 5 x 55 1 64 7 x 31 5 x 55 1 76 7 x 31 5 x 55 1 Gross Internal Dimensions 970 x 550x 670 mm 1870 x 550 x 670 mm WxDxH 38 2 x21 7 x 26 4 50 0 x21 7 x26 4 61 8 x21 7 x26 4 73 6 x 21 7 x 26 4 Usable Work Area 0 85 m 9 1 sq ft Avg Airflow Inflow 0 45 m s 90 fpm at initial setpoint Velocity Downflow 0 30 m s 60 fpm at initial setpoint with uniformity of better than 2096 Inflow 270 m h 162 cfm 356 h 213 cfm 440 m h 263 cfm 524 h 313 cfm Downflow 563 h 337 cfm 741 h 443 cfm 916 h 548 cfm 1091 m h 652 cfm Exhaust 270 m h 162 cfm 356 h 213 cfm 440 m h 263 cfm 524 h 313 cfm Required Exhaust Airflow With Optional Volume Thimble Exhaust Collar Static Pressure For Optional Thimble 29 0 11 in H20 31 Pa 0 12 H20 35 Pa 0 14 in H20 47 Pa 0 18 in Exhaust Collar ULPA Filter Downflow gt 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 3 USA Efficiency Exhaust gt 99 999 at MPPS as per EN 1822 H 14 EU sound JANSA Emission EN12469 Fluorescent Lamp Intensity gt 1040 Lux gt 1190 Lux gt 920 Lux gt 1020 Lux gt 97 foot candles gt 111 foot candles gt 85 foot candles gt 95 foot candles Cabinet Main Body 1 2 mm 0 05 18 gauge electro galvanized steel with white oven baked epoxy polyester Construction Isocide antimicrobial powder coated finish Work Zone 1 5 mm 0 06 16 ga
11. 2 19 8 Two steps of tightening are necessary with the first round torque strength of one third of the final torque required Over tightening may cause damage to filter and cause leak 1 Before the filters are removed from the BSC their contaminated faces should be taped off using plastic film or cardboard or some other suitable material This will minimize the risk of personnel in the event the decontamination was not thorough 2 Usedfilters should be disposed following local regulations They may have to be incinerated as medical waste They should be double bagged and appropriately labeled following removal from BSC 3 Proper personnel protective attire should be worn when removing used filters a disposable gown gloves facemask hair and shoe covers are appropriate These should be disposed after the procedure Proper hand washing after the procedure is also necessary 4 Before the new filters are installed all surfaces should be thoroughly cleaned of silicon and or adherent gasket material The new filter should be carefully handled and examined prior to fitting It is important that the filters and the gaskets be checked for leaks prior to use Class Biological Safety Cabinets Ecco 5 2 1 1 Filter Replacement for AC2 _E_ and 2 5 Series Remove screws from the bottom 2 sides of front panel Lift up the front panel and hook up the 2 support bar housing and lift up
12. Net Weight 213 kg 470 lbs 310 kg 683 Ibs Sound test is conducted an open field condition anechoic chamber PS Cabinet only excluding retrofit kit amp support stand ECCO Airstream APPENDIX 4A for 2 3 4 5ft 6A for 6ft CABINET POWER IN N gt j 54 2 O O EQR EL CB 2 for 2 3 4 5 EQR EL IEC320 C14 Note 1 Power supply 220 240VAC 50Hz 60Hz single phase 2 8uF Capacitor for 50Hz 3 10uF Capacitor for 60Hz 27 Blower only for 6 EQR EL CB 3 for 6 EQR EL BLOWER 14 EQR EL BLOWER 14 3 E 22 5 S Pd oos som BLOWER x x x X 6 MOTOR 13 14 11 9 1 SPEED CONTROL 1 2 9 5 EST M d MINE 4 I1 BL ee m 4 74 Lo S S S A EQR EL TB DFT EQR EL KB KBWC 26R for 2 3 4 5 mi EQR EL KH7060155 065 for 6 i 12 58 2 BLOWER CONTROL AAA 54 lt vi Ki 20 19 18 Motor voltage GER test point en BRN ass BLK 57 adole ze T et 1 NON POLAR CAPACITOR EQR EL CCAP SuF for 50Hz BK EORVEL CCAP 1OUF for 60Hz BLOWER CONTROL COIL E for 50Hz EQR EL CCAP 10u
13. 1 6 board or from e Voltage should be between 6 75 8 25VDC main board to e If voltage is out of range or no voltage check connection between SMPS and main display board e Check flat ribbon cable connection from main board to LCD keypad membrane NOTE There are two ribbon cables from the main board and the display and key pad The larger flat cable is for the Display The smaller flat cable is for the LEDs keypad membrane e Ifthe LCD Display is on but not the LEDs in keypad membrane check connection on the smaller Flat Ribbon cable e If incoming voltage at J13 is correct and flat ribbon cable connections are connected but the LEDs on the keypad membrane and LCD display are still not on proceed to next step mI Figure 1 6 Defective main e The main board is defective if the main board incoming supply is between 6 75 board 8 25VDC and o All LED s on the control panel are off o TheLCD is blank o No buzzer sound If these conditions exist replace the main board otherwise proceed to next step Note when replacing main board reconnect all wires correctly any wrong wiring may result in damage Class Biological Safety Cabinets Ecco Problem 2 Blank LCD Corrective Action Connection Check LCD large Flat Ribbon Cable connecting the main board with the display at the problem main board end Check if the cable has been inserted properly into its socket on the main board This is a Keyed connector with
14. 14 61 14 62 14 63 G Y 16 64 Black 16 65 Blue 16 66 Brown 16 SMPS NO COLOR AWG 101 Black 18 102 White 18 103 Green 18 Fluorescent Ballast NO COLOR AWG 107 Black 18 108 White 18 109 Red 18 110 Red 18 111 Blue 18 112 Blue 18 113 Yellow 18 114 Yellow 18 115 Yellow 18 116 Yellow 18 117 Red 18 118 Red 18 119 Blue 18 120 Blue 18 121 Yellow 18 122 Yellow 18 123 Yellow 18 124 Yellow 18 UV Ballast NO COLOR AWG 161 Black 18 162 White 18 164 Red 18 165 Red 18 166 Yellow 18 167 Yellow 18 173 Red 18 174 Red 18 175 Blue 18 176 Blue 18 Socket Outlet NO COLOR AWG 191 Black 16 192 White 16 193 Green 16 194 Black 16 195 White 16 196 Green 16 215 Black 16 216 White 16 217 Green 16 218 Black 16 219 White 16 220 Green 16 Blower NO COLOR AWG 239 Black 16 240 Blue 16 241 Brown 16 242 GN 16 243 Black 16 244 Blue 16 245 Brown 16 246 16 Ground Wires Connector A B C D pape Connector X9 X10 MOCCA Connector 11 12 X13 15 000 mem F M ECCO WORLD CLASS WORLDWIDE ELECTRICAL CIRCUIT DIAGRAM MODEL AC2 E S1 3 G2 DWG TITLE EP A AC2 E S1 3 G2 CHECKED REL DATE REMARK INDRAW INDRAS 2010 10 28 6A for 2 3 4 5 us 10A for 6 N 1 1 EQR
15. 20 3 2 1 Turning on the BSC 20 3 2 2 Turning off the BSC 20 3 3 Working in the BSC 21 3 4 Working Ergonomics 22 3 5 UV Lamps 22 3 6 Decontamination and Disinfecting Agents SERVICE SECTION Chapter 4 Installation 4 1 General Requirements x 4 1 1 Location Requirements 27 4 1 2 Environmental Requirements 27 4 1 3 Support Requirements 27 4 1 4 Exhaust Requirements 27 4 1 5 Electrical Requirements Class Il Biological Safety Cabinets Ecco 28 4 1 6 Service Line Requirements 28 4 2 Installation 28 4 2 1 Connecting the Electrical Supply 28 4 2 2 Connecting to a PC 29 4 2 3 Safety Labels on the Fume Hood 29 4 2 4 Preliminary Cleaning 29 4 3 Performance Validation Certification 29 4 3 1 Disclaimer 29 4 3 2 References for Qualified Certifiers 31 5 1 Scheduled Maintenance 33 5 2 Parts Replacement 33 5 2 1 Filter Replacement Procedure 35 5 2 2 Blower Replacement Procedure 35 5 2 3 Fluorescent Lamp s Replacement Procedure 35 5 2 4 UV Lamp Replacement Procedure 36 5 2 3 Airflow Sensor Replacement Procedure 36 5 3 Maintenance Service Log 37 6 1 Certification Flowchart 37 6 2 Certification and Calibration 38 6 3 Airflow Adjustment 37 6 3 1 Speed Controller Adjustment 37 6 3 2 Initial Voltage Adjustment 39 6 3 3 Damper Adjustment 41 7 1 Decontamination Agents 41 7 1 1 Formalin Paraformaldehyde Decontamination 42 7 1 2 Chlorine Dioxide Decontamination 42 7 1 3 Hydrogen Peroxide Decontamination 43 7 1
16. 3 Pin 5 is ground If you have voltage at Connector A but not at the RED LIVE and BLUE NEUTRAL terminal blocks the problem is between connector A and the terminal strips If voltage is not present at connector A BUT is present at the female connector for the line cord at the top of the cabinet that the power cord is plugged into the problem is between the female connector and the pins on Connector A Figure 1 2 m Figure 1 3 E co Airstream Defective power e Locate the SMPS Power Supply and 5 pin connector supply Switch e Onthe BACK side of the 5 pin connector measure the DC voltage between Red Mode Power 1 and White pin 2 Measure on the side away from the SMPS See Figure 1 4 supply SMPS e The voltage should be in range of 7 5VDC 10 e If you do not have voltage measure the other side of the 5 pin connect e foutofrange or no voltage check incoming power to the SMPS Molded cord check terminal where the cord is connected The input of SMPS should be 10 of the cabinet incoming voltage e If you have input voltage but no output voltage replace the SMPS e If input and output voltage is correct proceed to next step SMPS input 4 115V or Connector SMPS output 230V AC dla ES red white is 75VDC Figure 1 5 Figure 1 4 Connection e Measure the incoming voltage on the main board at terminal J 13 Note polarity problem to main blue cable closest to edge is negative See Figure
17. 4 8 Reset Default User can reset the default setting by choosing this option The features being reset are warm up period 3 minutes post purge period 0 minute UV timer 60 minute measurement unit Metric Airflow monitor enabled ADMIN PIN 0009 Filter Life Display disabled and FAN PIN 0000 Note that the calibration settings cannot be reset as it may cause the BSC to operate in an unsafe manner The hour meters cannot be reset either MENU p ADMINSETTINGS 5 RESET DEFAULT To Reset Default 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose RESET DEFAULT Press SET button to confirm The display will Show READ MANUAL and PRESS SET Press SET button to confirm The display will Show CONFIRM Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display e Class Biological Safety Cabinets Ecco 2 2 4 9 Airflow Out Temp Using this option the user can select the whether or not to display the airflow when the ambient temperature is out of optimum temperature range below 18 C 65 F or
18. ADC ADC value for Fail Point Inflow calculated using offset based on Inflow Nominal Point ADC IFN ADC value for Nominal Point Inflow set during sensor calibration ADC IFA ADC value for Actual Inflow showing real time sensor reading ADC IFO ADC value for factory calibrated Zero Point Inflow no inflow set during zero calibration ADC IF1 ADC value for factory calibrated Fail Point Inflow ADC IF2 ADC value for factory calibrated Nominal Point Inflow ADC DFA ADC value for Actual Downflow showing real time sensor reading ADC DFO ADC value for factory calibrated Zero Point Downflow no downflow ADC DFN ADC value for Nominal Point downflow based on field calibration CONSTANT value for Inflow sensor constant keyed in during sensor calibration CALIB TEMP Temperature when the factory calibration was performed ADC TEMP ADC value for temperature Note Depending on preset unit at SETTINGS gt MEASUREMENT UNIT gt Metric or Imperial the units for temperature and velocity will change accordingly For Metric C m s while for Imperial F Factory setting is at Metric In order to prevent any AIRFAIL or unstable velocity displayed on LCD problem please ensure the following is done after the calibration 1 CONSTANT value is correct Check it with constant written on airflow sensor body or inside Replaceable Component Record List which comes with the test report 2 TEMP
19. Certification and Calibration Certification and calibration procedure can be found in the test report Class Biological Safety Cabinets Ecco 6 3 Airflow Adjustment 6 3 1 Speed Controller Adjustment The speed controller is located in the electronics panel which is accessed by raising the front engineering access panel at the top of the front of the cabinet and then opening the drop down cover by removing the two screws on the front left and right sides of the cover Speed Controller Plug the multimeter probes to the Motor Voltage Sampling Port Use the multimeter to take the voltage air velocity reading Adjust the airflow by adjusting the speed controller Use the provided screwdriver to adjust the controller 6 3 2 Initial Voltage Adjustment only for AC2 D and 2 series These AC2 BSCs are equipped with an exhaust fan below of the exhaust filter This fan needs to be adjusted so that the correct balance between downflow velocity and inflow velocity is achieved Faster increase in value Increase greatly in value Constant Slower decrease in value Decrase greatly in value The BSC downflow and exhaust fan speed control need to be adjusted to get nominal downflow velocity of 0 30 m s E co Airstream 6 3 3 Damper Adjustment The damper is used to balance the downflow velocity and exhaust velocity The damper needs to be adjusted when the downflow velocity does not meet the accepted
20. EL CB 3 for 2 3 4 5 EQR EL CB 4 for 6 7 22 x Power supply 110 120VAC 60HZ single phase CABINET POWER IN a las 31 41 6 2 Blower EOR EL IEC320 C14 EQR EL BLOWER 2 for 2 3 4 6 EQR EL BLOWER 29 for 5 Only for 6 Y P wo N e N 3 D CN Nag BLOWER 7 X xX X 6 MOTOR 14 EQR EL TB DFT 96 N 999 Morro a a EQR EL KB KBWC 15R for 2 3 4 5 co m 2 EQR EL KH7060155 065 for 6 213111 17 6 LES S ll i 56 ex 23 19 18 T lt lt lt BLOWER CONTROL vow y 22 CONTACT X13 3 22 63 7 Ki Motor voltage Ka no fect point Al lt CP2NON POLAR CAPACITOR BRN sz BLK 5 50 een ine 1 NON POLAR CAPACITOR EQR EL CCAP 20uF 53 BLK BLK BLOWER CONTROL COIL EQR EL CCAP 20uF for 2 3 4 6 54 EQR EL CCAP 30uF for 5 _ 198 u O EQR EL REL JQX 30A 1 107 FLUORESCENT BALLAST EQR EL MC 28 EQR EL BAL UNV T8 IS EQR EL MC 19 ejeje 32 ESC 2 RELAY BOARD F17T8 2 F25T8 3 F32T8 4 gt gt UVCTRL RECEP CTRL 8 8 LS 5 LS 8 LS 4 m MEN S 162 EQR EL TUB UVG15T8 for 2 3 N n EQR EL TUB UVG30T8 for 4 5 6 me 164 B 7 173 B 8 UV LAMP yO 167 5 7 5 1 e 1656 7 pe
21. FL CTRL BLOWER CTRL 2 2 2 161 UV BALLAST id 5 166 er Isi s s S s 26 B K2 UV INTERLOCK CONTACT E 25 3l EQR EL UVBALLAST 7 coml Ino 26 29 DC POWER 322 B2A A B5 EQR EL MC 13 47 5 RED x122 COM T 12 54 39 EQR ELRELJOCI2VD D 3 a MAGNETIC EQR EL MC 22 40 D 6 WHT UV INTERLOCK 102 UV INTERLOCK RELAY COIL EQR EL OUTLET GFCI EQR EL OUTLET GFCI RS232 PORT EOR EL SA TS NEAR 20 DB9F 9 PIN POWER INLET uj RED X15 1 RED GRN X15 2 GRN B BLK X15 3 BLK amp Z EQR EL SA B2 for 2 RS232 PORT HO EQR EL SA B1 for 3 4 5 6 N E Y 0 5 WHT gt D5 WE a MAGNETIC 111 42 SD4 WAT UV SW pIN2 p Pre urn 43 0 2 m 2 44 D aa uu BOARD AL AIN4 L o a El dp MAGNETIC 0 E dcs A s gt D 8 WAIT OPEN SW EQR EL MC 35 1080474 AL 51 52 53 54 EQR EL MC BS2011 UL WORLD CLASS WORLDWIDE ESC3 ESC4 LED MEMBRANE j wa an 22AWG ELECTRICAL CIRCUIT DIAGRAM ESC6 EQR EL MC 18 36 INTERFACE A 4 RA EQR EL MC 20 EQR EL MC 11 EQR EL MC 13 MODEL 2 2 2 AC2 3E2 AC2 4E2 AC2 5E2 2 6 2 2 252 AC2 352 2 452 2 552 2 652 MODEL 2 52 GENERATION 2 DWG TITLE EP C 2 E S2 G2 CHECKED Ret INDRA S PAUL T 2010 11 29 REV
22. Formaldehyde 1910 1048 Occupational Safety and Health Standards OSHA Occupational Safety and Health Administration U S Department of Labor http www osha gov e OSHA Formaldehyde Fact sheet PDF format http www osha gov OshDoc data General Facts formaldehydefactsheet pdf Class Biological Safety Cabinets Ecco Process Time Set up amp sealing the BSC to make it air tight Formalin vaporization Formalin contact time to obtain target log of 4 6 kill Ammonia vaporization to neutralize formalin Ammonia contact time to neutralize formalin Exhausting the ammonia residue Tear down amp cleaning the substantial residue TOTAL without ammonia neutralization TOTAL with ammonia neutralization Ammonia is used to neutralize formaldehyde OSHA prescribes the ammonia STEL is 35 ppm for 15 minutes exposure 4 times a day minimum of 60 minutes in between exposure Due to the adverse health effect of formalin gas its use has been banned in Germany Austria and Switzerland Other European countries are expected to follow suit Two primary candidates to replace formalin decontamination are chlorine dioxide gas and hydrogen peroxide vapor 7 1 2 Chlorine Dioxide Decontamination Chlorine dioxide decontamination is performed by injecting chlorine gas Cl into a cylinder filled with solid sodium chlorite which generates the greenish yellow chloride dioxide gas Chlorine dioxide decontamination is much fast
23. If fuse F4 is OK check the voltage between the BLUE NEUTRAL terminal block and the end terminal COM on the 154 circuit J6 on relay board Voltage at the end terminal Normally Closed circuit with no cable indicates the F4 fuse is good and the circuit is not energized Make sure the OUTLET LED is on If there is power to the relay and it is energized Check voltage across Connector B pin 1 to 2 and pin 4 to 5 If there is power through Connector B pins remove socket outlets from wall Check incoming power to the plug in the cable to the outlet No power indicates a wiring issue Recheck voltage from Connector B pins Check for loose or faulty connection between the power cord IEC inlet and Connector B Relay LS4 RELAY BOARD Figure 9 1 Faulty electrical e Socket is located inside the work zone socket e Check electrical socket connection e If connection is correct but socket has no output replace electrical socket E co Airstream Problem 10 Contaminated Sample Lab Corrective Action Blocked air grille Note Contaminated samples are normally the result of any of the following Cabinet overloaded Poor Technique of the user or lack of training with too many Samples being contaminated in the incubator or somewhere other than the BSC items Items to check on the BSC Make sure that the front and back air grilles are not blocked Remove all equipment and other items from the cabinet and wipe down the cabinet i
24. Press SET button to confirm Use UP DOWN buttons to choose RESET UV H M Press SET button to confirm The display will Show READ MANUAL and PRESS SET Press SET button to confirm The display will show CONFIRM Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display ee 2 2 4 7 Reset F H M This option is used to reset the filter hour meter The filter hour meter indicates how long the filter has been in operation Maximum counter is set at 10000 hours 10096 The counter value can be checked while in the diagnostic mode Please reset the filter hour meter after each filter replacement MENU p ADMIN SETTINGS RESET F H M To Reset F H M 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose RESET F H M Press SET button to confirm The display will Show READ MANUAL and PRESS SET Press SET button to confirm The display will Show CONFIRM Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display d I 2 2
25. SENSOR UNCALIBRATED will be displayed if the airflow velocity sensor is not yet calibrated E co Airstream 2 5 Diagnostic Mode Diagnostic mode can be accessed by pressing the SET button The diagnostic mode allows the user to know the condition of the BSC or help the service engineer during maintenance and troubleshooting On Screen Explanation Zu ADC IFO ADC for factory calibrated Zero Point Inflow no inflow ADC ADC for factory calibrated Fail Point Inflow UV TIMER Shows the UV timer value default is 60 minutes Maximum value is 00 minutes infinite on ADC IFN ADC for Nominal Point Inflow based on field calibration ADC IF2 ADC for factory calibrated Nominal Point Inflow ADC IFA ADC for Actual Inflow showing real time sensor reading ADC IFF ADC for Fail Point Inflow calculated using offset based on Inflow Nominal Point Class Biological Safety Cabinets Ecco E co Airstream Chapter 3 Basic Cabinet Operation 3 1 Sash Window Operation 3 1 1 Sash Window State Sash is fully open Sash is in safe position Sash is fully closed v Blower can be active v Blower can be activated x Blower can t be activated v Fluorescent lights can be used Y Fluorescent lights can be used Fluorescent lights can t be used x Unsafe working condition Y Safe working condition 3 1 2 Operating Motorized Sash Window Only for BSC with motorize
26. Singapore e sco offers field certification services directly Contact local Esco office Other Countries e Contact Esco or local distributor Class Biological Safety Cabinets Ecco E co Airstream Chapter 5 Service and Maintenance 5 1 Scheduled Maintenance Proper and timely maintenance is crucial for trouble free functioning of any device and your Esco BSC is no exception to this rule We strongly recommend that you follow the maintenance schedule suggested hereunder in order to obtain optimal performance from your Esco BSC No Description of Task to Perform Maintenance to be carried out every P Day Week Month Quarter 1Year 2 Years 1 Surface decontaminate the work zone 2 BSCpower upalarmverification wo 1 3 Thoroughly surface decontaminate the drainpan J OJo vw T 4 Check the paper catch for retained materials O Yo wo 5 Clean the exterior surfaces of the BSC Clean the sash window Check all service fixtures where present for proper operation Inspect the BSC for any physical abnormalities or malfunction 9 Clean up stainless steel surfaces with Re certification Change UV Lamp where present BE A A PE A 1 51 _ Exp Cleaning the BSC e Clean the work surface and walls with appropriate disinfectant agent and soap water a
27. System e The BIOQUELL principle is to seek micro condensation to achieve the kill The generator releases tiny high speed droplets inside the BSC BIOQUELL product for BSC is Clarus L Hydrogen peroxide vapor is non carcinogenic but highly effective against micro organisms Hydrogen peroxide 202 vapor breaks down under catalytic action to become air and water making it environmentally friendly and it leaves no residues The decontamination process is as fast as chlorine dioxide if the BSC is ducted However if the BSC is not ducted the hydrogen peroxide must be aerated which is time consuming The time needed for the entire process is outlined below E co Airstream Process Time Conditioning and decontamination cycle Ducting out H202 H O generator doing aeration For hydrogen peroxide decontamination the BSC need to be equipped with two ports 1 One port located in front opening or side wall penetrating the work zone area 2 One port located on top of the exhaust filter The generator used defines the port function as described below Steris Bioquell Hydrogen peroxide source Bottom front side port Top port 7 1 4 Comparison Table Aspect Formalin Vapor Chlorine Dioxide Hydrogen Peroxide lsitagenooxn o es NN Immediately Damaging to Life amp Health 2 ppm 5 ppm 75 ppm IDLH Sealing of BSC Must be airtight Must be airtight Small gaps are OK Lab evacuated during the process Yes due to leaka
28. above 30 C 86 F gt ENABLED MENU e ADMIN SETTINGS AIRFLOW OUT TEMP DISABLED To Set Filter Life Display 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose AIRFLOW OUT TEMP Press SET button to confirm Use UP DOWN buttons to choose between ENABLED and DISABLED Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display Ua s e a 2 3 Stopwatch and Experiment Timer e stopwatch function can be started by pressing the UP button while the sash is in the safe ready position Pressing UP button again while the stopwatch function will stop and resume the timer Pressing DOWN button will leave the stopwatch function and reset the timer The timer in the stopwatch function is counting up and shown using the HH MM SS format e experiment timer can be started by pressing the DOWN button while the sash is in the safe ready position Pressing DOWN button while the experiment timer function is working will stop and resume the timer Pressing the UP button will leave the experimen
29. after pressing UV button refer to the Magnetic Switch troubleshooting section ESO Airstream Faulty UV tube e Replace the faulty UV tube Faulty UV ballast e Check voltage coming to the Relay board e Locate 158 J15 Terminal circuit and measure AC voltage between large BLUE Neutral terminal block and the end terminal with Red cable on the three terminal strip J15 on relay board If there is NO Power to the end terminal Terminal with cable refer to the next step NO power to UV Relay circuit LS8 If there IS voltage to the end terminal Terminal with cable check the central terminal Normally Open Circuit to the BLUE Neutral terminal block No voltage at the central terminal indicates a blown fuse or the circuit is not energized Check Fuse F8 on relay board See Figure 8 1 below If fuse F8 is blown as temporary solution use F5 spare to replace F8 If fuse F8 is okay check the voltage between the BLUE NEUTRAL terminal block and the end terminal on the LS8 circuit on J15 terminal of relay board Voltage at the end terminal with no cable indicates the F8 fuse is good and the circuit is not energized Make sure the UV LED is on If the ballast has power and the UV lamp is new the problem is either cable connections or the UV ballast Check Connector B cable connections If there no loose terminals replace the UV ballast No Power to UV No power to LS8 indicates K2 Relay is open Relay circuit 158 Che
30. criteria 3 4 5 unit 0 35 m s and 6 unit 0 33 m s Open the damper opening if the downflow velocity is too high close the damper opening if the downflow velocity is too low Damper Thumbscrew Loosen the thumbscrew s to slide the damper left or right to increase or reduce the downflow velocity Class Biological Safety Cabinets Ecco E co Airstream Chapter 7 Decontamination 5 decontamination should be performed any of the following eventualities e Before replacing the exhaust downflow filters or blower e Before accessing the contaminated negative or positive plenum of the BSC e Incase of an accidental spillage that might have contaminated any of the inaccessible surfaces e Before performance validation and re certification especially if the BSC has been used with BSL 3 or 4 agents e Before BSC relocation Personal protective equipment such as gloves gown and respirator with filter suitable for the decontamination gas vapor must also be used by the certifier performing the decontamination Esco can provide the formalin vaporizer and decontamination bag needed for the decontamination procedure 7 1 Decontamination Agents 7 1 1 Formalin Paraformaldehyde Decontamination Typically the decontamination is performed using formalin gas by either vaporizing 37 formalin solution or by de polymerization of solid paraformaldehyde Despite its widespread usage for decontamination formal
31. shown in the HyperTerminal window are current time inflow velocity downflow velocity temperature sash window position blower hour meter and blower condition or warm up condition 4 2 3 Safety and Warning Labels on the Cabinet Anyone using the BSC should familiarize themselves with the various labels displayed in and on the cabinet It is very important that users are familiar with the meanings of the labels before attempting to use the unit 4 2 4 Preliminary Cleaning Wipe the interior and exterior of the BSC with water or a mild household detergent 4 3 Performance Validation Certification After the installation but before starting to use it BSC performance must be validated and certified to factory standards It is recommended that this validation and certification be performed only by a qualified technician who is familiar with the methods and procedures for certifying biological safety cabinets The test methods and equipment needed for carrying out the tests are specified on the test report 4 3 1 Disclaimer The performance and safety of all Esco BSC are rigorously evaluated at our factory Regular field certification is important to ensure factory standards are maintained 4 3 2 References for Qualified Certifiers North America e http www nsf org Certified Biosafety Certifier e Esco www us escoglobal com e AFCA member certifying company www iafca com listview UK China India Middle East North Africa Malaysia
32. to return to the main display quod e 2 2 4 2 New FAN PIN default 0000 DISABLED FAN PIN restricts access to fan control and some parts of the menu settings and set mode User must enter four digit PIN before switching fan on or off As such it can restrict access to operating the BSC by unauthorized personnel It will also prevent unauthorized shutdown of the BSC when continuous operation is required Note that continuous operation is recommended for better safety Fan PIN is also needed to disable the alarm when the sash is fully raised and cleaning needs to be performed It is recommended that the Fan PIN be issued only to personnel authorized to use the BSC With FAN PIN the user can access admin and set mode parts of the menu E co Airstream Setting the to 0000 will disable this feature is disabled by default When the is disabled the BSC can be turned on and off without requiring PIN however to access the menu the user is still required to enter the FAN PIN 0000 MENU gt ADMINSETTINGS NEW FAN PIN gt XXXX To Set New FAN PIN 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons t
33. to the maximum Remove the access cover Remove the exhaust filter mounting frame from the top Remove the exhaust filter Using the 13 spanner turn the nut until the blower housing is lifted up to the maximum Remove the filter from the front To install new filter reverse the above procedure and note the following A When securing blower housing onto new filter please ensure gasket compression is equal on both downflow and exhaust filter B When securing the exhaust filter mounting frame please do not over tighten the nuts use the lowest torque if using a power tool AN 5 2 1 2 Exhaust Filter Replacement for AC2 _D_ and AC2 _G_ Series ECCO Airstream Remove screws from bottom 2 sides of front panel 3 Lift up the front panel 3 and hook up the 2 support bar housing and lift up to the maximum Remove the access cover 4 Remove the secondary exhaust filter mounting frame 1 from the top Remove the secondary exhaust filter 2 Remove the primary exhaust filter 5 from the front To install the new filter reverse the above procedures EN DER 5 2 1 3 Downflow Filter Replacement for AC2 _D_ and AC2 _G_ Series Follow step 1 6 on the AC2 D and AC2 G series exhaust filter replacement Continue with the following steps 7 Using the spanner turn the nut until the blower plenum 7 is lifted up to the maximum 8 Remove the downflow filter 6 from the front 9 Toinstall the new filter reverse the above procedures
34. 2 G series the sensor is located between the primary and secondary exhaust filter To access the sensor in these cabinets you will need to remove the secondary exhaust filter and its frame Do not forget to decontaminate the BSC before removing the filters Start by disconnecting the airflow sensor cable connector Cut the cable ties to remove the temperature sensor Unfasten the nut connecting the airflow sensor to the sensor housing Pull out the old airflow sensor from the sensor housing Push in the new sensor Make sure that the arrow sticker on the sensor tube is pointing up as shown in the figure below so that the axes of the sensor holes are parallel to the airflow direction Also make sure that the distance between the outer sensor hole and wall of the sensor housing is 5 5 cm as shown in the next figure pe one Dus Air Flow Direction Ensure correct distance Ensure correct orientation 6 Fasten the nut and reconnect the airflow sensor cable connector Mount back the cable onto the sensor housing Make sure that there is no gap between the housing and the filter as shown below yri When installing the airflow No gap between sensor please the casing and watch the airflow A A filter direction 5 3 Maintenance Service Log It is good practice and in some cases regulatory requirement to maintain a log of all maintenance work carried out on your cabinet E co Airstream Chapter 6 Rec
35. 2 3E2 AC2 4E2 AC2 6E2 19 21uF 2 252 2 352 2 452 2 652 19 21 Pues AC2 5E2 2 552 28 32uF the capacitor by Note 6 feet models have 2 pcs of capacitor 1 for additional blower required using multimeter Figure 4 9 De Class Biological Safety Cabinets ECCO Auto thermal cut off Motor failure Check the Fan for overheating The motor has a thermal cut out built in Wait 60 minutes with the FAN turned off and then try to restart If the FAN restarts determine why there is excessive heat in the cabinet If the FAN does not restart proceed to the next step WARNING The cabinet MUST be decontaminated before opening the Red Panel Check for physical damage Will the motor rotate Check wiring Replace the blower Problem 5 Airflow Failure AIRFAIL alarm is triggered Cause External air interference Blocked cabinet grille Low building supply voltage if new cabinet check this first Defective airflow sensor power supply Switch Mode Power supply SMPS Cabinet requires the air flows to be set Filter loaded or wrong speed controller setting Corrective Action Ensure that there are no external sources of airflow disturbance close to the sensor Check vents lights ceiling too close etc If there is no external air interference proceed to next step Ensure that the air grilles inside the cabinet are not blocked and that the
36. 2 5E2 AC2 6E2 AC2 4E3 AC2 6E3 AC2 3E3 AC2 453 AC2 5E3 AC2 653 AC2 353 AC2 4D3 AC2 5S3 AC2 6D3 AC2 4G3 AC2 6G3 Electrical Rating 220 240 V AC 50Hz 10 2 2 1 220 240 V AC 60Hz 10 2 2 2 Safety Warning e Anyone working with on or around this equipment should read this manual Failure to read understand and follow the instructions given in this documentation may result in damage to the unit injury to operating personnel and or poor equipment performance e Any internal adjustment modification or maintenance to this equipment must be undertaken by qualified service personnel e The use of any hazardous materials in this equipment must be monitored by an industrial hygienist safety officer or some other suitably qualified individual e Before you process you should thoroughly understand the installation procedures and take note of the environmental electrical requirements e Inthis manual important safety related points will be marked with the symbol e fthe equipment is used in a manner not specified by this manual the protection provided by this equipment may be impaired 3 Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various local regulations Familiarization and compliance with any such regulations are the sole responsibility of the users Esco s liability is limited with respect to user compliance with such regulations
37. 4 Comparison Table 43 7 2 Recommended Decontamination Sealing Method DEBES Chapter 8 Troubleshooting DEBE Chapter 9 Engineering Details 65 9 1 2 Series 65 9 1 1 Engineering Drawing 66 9 1 2 General Specification 67 9 2 2 5 Series 67 9 2 1 Engineering Drawing 68 9 2 2 General Specification 69 9 3 AC2 _D_ Series 69 9 3 1 Engineering Drawing 70 9 3 2 General Specification 71 9 4 2 G Series 71 9 4 1 Engineering Drawing 72 9 4 2 General Specification APPENDIX ECCO Airstream Warranty Terms and Conditions Esco warrants that the product described in this manual will perform according to specifications for a period of 36 months from the date of purchase Esco s limited warranty covers defects in materials and workmanship During the warranty period Esco s liability shall be at our option to repair or replace any defective parts of the product provided if proven to the satisfaction of Esco that these parts were defective at the time of being sold and that all defective parts shall be returned properly identified with a Return Authorization This limited warranty covers parts ONLY and not transportation insurance charges This limited warranty does not cover e Freight or installation inside delivery handling damage If your product was damaged in transit you must file a claim directly with the freight carrier e Products with missing or defaced serial numbers e Products for which Esco has not receiv
38. 5 minutes to reach 30 000 uW cm2 1 W 1 J sec which has been listed as germicidal for spore forming organism Unlike many other type of decontamination agent UV light doesn t leave any residue The decontamination action stops upon de energizing of the lamp However due to the short wavelength the UV light does not penetrate well Thus UV light can only be used to effectively disinfect the work area of an empty BSC For any container stored inside the BSC s work area the UV radiation will only disinfect the outer surface of the material leaving the inner surface and the content inside the material untouched e UV light decontamination method may be used before and after working with vegetative organisms and viruses However it should not be the sole decontamination agent the use of chemical decontamination agent is still encouraged e Minimize the material inside the BSC s work area during the process of UV light decontamination A direct interaction with UV light can degenerate plastic or rubber based material and can cause other hazard e Make sure that the BSC sash is in the fully closed position and the interlock is working properly before activating the UV lamp Avoid direct contact with skin and eyes as UV light is classified as a probable human carcinogen TheUV lamp should be activated for around 60 minutes to work effectively Use the UV timer feature to easily control the period of decontamination UV timer is disabled by defa
39. 61 Black 18 162 White 18 164 Red 18 165 Red 18 166 Yellow 18 167 Yellow 18 173 Red 18 174 Red 18 175 Blue 18 176 Blue 18 Socket Outlet NO COLOR AWG 191 Black 16 192 White 16 193 Green 16 194 Black 16 195 White 16 196 Green 16 215 Black 16 216 White 16 217 Green 16 218 Black 16 219 White 16 220 Green 16 Blower NO COLOR AWG 239 Black 16 240 Blue 16 241 Brown 16 242 G Y 16 243 Black 16 244 Blue 16 245 Brown 16 246 G Y 16 Ground Wires Connector X9 X10 n Q 5 F Connector 11 12 13 15 1004 F M ECCO WORLD CLASS WORLDWIDE ELECTRICAL CIRCUIT DIAGRAM MODEL AC2 E S2 GENERATION 2 DWG TITLE EP C AC2 E S2 G2 CHECKED REL DATE REMARK INDRA S PAUL T 2010 11 29 LOG RECORD Cabinet Serial Number Person in Charge 1 This log record should be used by the operator to record any new agent virus bacteria germs that has been introduced to the cabinet during its operation problems encountered etc 2 Anydecontamination procedure performed by either the user or the technician should be recorded down as well 3 Please also record any major maintenance procedure performed by the service technician for example parts replacement recertification etc User Signature Supervisor Signature In case of emergencies please call Name CN Cell Phone Number E mail
40. AS EXPIRED ESCO DOES NOT ACCEPT LIABILITY BEYOND THE REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL INDIRECT CONSEQUENTIAL OR INCIDENTAL DAMAGES INCLUDING WITHOUT LIMITATION ANY LIABILITY FOR THIRD PARTY CLAIMS AGAINST YOU FOR DAMAGES FOR PRODUCTS NOT BEING AVAILABLE FOR USE OR FOR LOST WORK ESCO S LIABILITY WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE SUBJECT OF A CLAIM THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and shall be subject to the exclusive jurisdiction of the courts of Singapore Technical Support Warranty Service Contacts Toll Free USA and Canada 877 479 3726 Singapore 65 6542 0833 Global Email Helpdesk support escoglobal com Visit http www escoglobal com to talk to a Live Support Representative Distributors are encouraged to visit the Distributor Intranet for self help materials Policy updated on 30th Jan 2007 This limited warranty policy does not apply to products purchased before 30th Jan 2007 Class Il Biological Safety Cabinets Ecco ECCO Airstream Introduction 1 Products Covered Esco Class Il Biological Safety Cabinet Airstream 0 6 meters 0 9 meters 1 2 meters 1 5 meters 1 8 meters 2 feet 3 feet 4 feet 5 feet 6 feet AC2 4E1 AC2 6E1 AC2 3E1 2 451 AC2 5E1 AC2 6S1 AC2 3S1 AC2 4D1 2 551 AC2 6D1 AC2 4G1 AC2 6G1 AC2 3E2 AC2 4E2 AC
41. Display indicate SASH OK or SASH NO SASH NO indicates the sash is not at the proper position and the LIGHT LED should not be on This indicates a main board problem or a magnetic switch detection problem To access the magnetic switches for the sash positions remove the Right sash window profile cover as you face the cabinet There are 3 magnetic switches inside the right profile See Figure 7 2 below The one in the middle is for Ready position There is a magnet attached to the glass beside the middle switch Shift the switch or magnet position so the distance between them is between 10 13 mm 3 8 to 1 2 as explained in Figure 7 2 below If the distance is too far the switch may not be able to detect the magnet If the LCD still displays SASH NO the switch may be defective or there is a wiring connection problem To check the connections proceed to the next step Figure 7 2 Connection e Locate Connector C and D and check male and female side for any bad connection Problem e Ifthe fluorescent lamp still does not light with the LIGHT LED ON return to start of Problem 7 and repeat the troubleshooting process Problem 8 UV Light always OFF Corrective Action Sash not in UV Lower the sash to UV mode position state If SASH FULLY CLOSED is not displayed on the LCD please refer to the Magnetic Switch troubleshooting section Switch on the UV lamp by pressing the UV button If SASH FULLY CLOSED is displayed on LCD but UV is not ON
42. ERATURE shows room temperature If not refer to next possible cause part B 3 ADCIFO ADCIF1 ADC IF2 If not please re do calibration in correct sequence SET CONSTANT gt SET ZERO gt CALIB If CALIBRATION ERROR is encountered please refer to next possible cause part A 4 ADC DFO ADC DFN If not please re do calibration in correct sequence SET CONSTANT SET ZERO gt CALIB If CALIBRATION ERROR is encountered please refer to next possible cause part A 5 Just after calibration ADC IFA value should be close to ADC IFN value If not please adjust the speed controller to make both values closer by at least 20 units Sensor Failure A Air Flow Sensor failure misalignment Sensor e Airflow sensor the top of the cabinet E S series model or inside the unit below the Misalignment top filter D G series model Airflow sensor for D G series models can be accessed from the back of the unit See Figure 5 1 below It is a stainless steel tube with 2 round holes facing up Class Biological Safety Cabinets Ecco Important to check 1 Ensure that the sensor area is not blocked at all and that its through holes are perpendicular to the filter s surface Exhaust sensor is attached tightly to the box There is no gap between sensor box and filter surface Sensor box is installed tightly to the cabinet Turn off the cabinet Open the front panel and locate electrical box behind it Open electrical box and see Component
43. F for 60Hz EQR EL REL JQX 220V F17T8 2 F25T8 3 F32T8 4 109 117 121 2 T8 FLUORESCENT LAMP H1 1 T8 FLUORESCENT LAMP H2 FLUORESCENT p A eee BALLAST 111 C7 119 123 8 116 T8 FLUORESCENT LAMP EQR EL MC 28 EQR EL BAL UNV T8 IS EQR EL MC 19 Ji3 e e 12 ESC 2 RELAY BOARD gt UVCTRL RECEP CTRL LS 8 LS 4 _ _ EQR EL TUB UVG30T8 for 4 5 6 EQR EL TUB UVG15T8 for 2 3ft 165 B 10174 2 176 11167 6162 E 73 UV LAMP 175 Q 161 164 Bp 2 2 UV BALLAST Z E xo s e e gt 15 sTsTe 36 g K2 UV INTERLOCK CONTACT EQR EL UVBALLAST 6 102 Ino 26 2 1 4 2 48 5 191 32 194 X11 1 215 s up 4155 2 E i Se AAA DC POWER 0216 11 2 12 1 849 2 SUPPLY 217 11 3 220X12 33 X122 NIE 193 96 10 1 B 3 B 6 Socket Outlet 27 Socket Outlet 0 a 30 com WHT EQR EL OUTLET _ EQR EL OUTLET _ m UV INTERLOCK S4 12 GR 39 RELAY COIL D 3 de 95 Ta MAGNETIC EQR EL MC 22 40 5 _ UV INTERLOCK 103 EQR EL REL JQX 12VD RS232 PORT EOR EL SA TS POWER INLET X15 1 RED E GRN 52 B ae Q3 BIK 59 EQR EL SA B2 for 2 5 or 1185232 PORT EQR L SA B1 for 3 4 5 6 3 la os gt gt n uo 11 1 D a MAGNETIC ZE 53 un n0 1 4 D oa MAGNETIC gt Q I 2 READYSW amp MAIN BOARD AIN4 1080474 5 4 MEMBRANE EQR E
44. FAN should start If the FAN does not operate proceed to next step If the LED on the cabinet front does not illuminate refer to Problem 1 DC Voltage Measure the incoming voltage on the Relay Board at terminal J 1 Note polarity Connection BLUE cable closest to edge is negative See Figure 4 1 problem to relay Voltage should be between 6 75 8 25VDC board If voltage is out of range or no voltage check connection between SMPS power supply and relay board If voltage is correct proceed to next step Class Biological Safety Cabinets Ecco Fuse 1 is blown or Check AC voltage coming to the Relay board circuit is not Ensure the Motor Blower FAN button and LED is energized energized Locate LS1 circuit and measure AC voltage between large BLUE Neutral terminal block and the end terminal with Red cable on the three terminal strips J2 on relay board See Figure 4 1 If there is voltage to the end terminal Terminal with cable check the central terminal Normally Open Circuit to the BLUE Neutral terminal block No voltage at the central terminal indicates a blown fuse or the circuit is not energized Check Fuse F1 on relay board See Figure 4 1 below If fuse F1 is blown as temporary solution use F5 spare to replace F1 If fuse F1 is okay check the voltage between the BLUE Neutral terminal block and the end terminal on the LS1 circuit J2 on relay board Voltage at the end terminal with no cable indicates the F1 f
45. L MC 35 ESC3 LCD INTERFACE AAA B EQR EL MC 20 EQR EL MC 11 EQR EL MC 13 MODEL AC2 2E1 AC2 3E1 AC2 4E1 AC2 5E1 AC2 6F1 2 2 AC2 3E3 2 4 AC2 5E3 AC2 6E3 51 52 53 54 EQR EL MC BS2011 UL X9 5 _ MAGNETIC OPEN SW 22AWG ECCO WORLD CLASS WORLDWIDE ELECTRICAL CIRCUIT DIAGRAM MODEL AC2 E S1 3 G2 2 351 2 451 2 551 2 651 2 353 2 453 AC2 5E3 2 653 COLOR AWG 1 Black 14 2 White 14 3 Green 14 A Red 14 5 Blue 14 6 GN 14 7 Red 14 8 Red 14 9 Red 14 10 Red 14 11 Red 14 12 Red 14 13 Blue 14 14 Blue 14 15 Blue 14 16 Blue 14 17 Blue 14 18 Red 14 19 Red 16 20 Red 16 21 Red 16 22 Red 16 23 Blue 16 24 Red 16 25 Blue 16 26 Red 16 27 G Y 16 28 Red 16 29 Blue 16 30 G Y 16 31 Red 16 32 Red 18 33 Blue 18 34 Red 18 35 Blue 18 36 Red 18 37 Black 18 38 Black 18 39 Red 18 40 Red 18 41 Brown 18 42 Brown 18 43 Yellow 18 44 Yellow 18 45 Blue 18 46 Red 18 47 Red 18 48 Black 18 49 Yellow 18 50 Red 14 51 Black 18 52 Blue 18 53 Brown 16 54 Red 14 55 Blue 16 56 Blue 14 57 Black 14 58 Red 14 59 14 60
46. Layout at the beginning of this section to locate the main board inside electrical box Using multimeter check the exhaust sensor s output voltage DC at channel AIN1 and J1 on main board See Figure 5 2 below The airflow sensor voltage should increase if airflow is increased and decrease if airflow is decreased If your observations are different check airflow sensor connection on top of the unit see Figure 5 1 and at connector D pin 9 10 11 12 on electrical panel for any bad loose connection If connections are okay but sensor is still not working properly replace it If airflow sensor is working properly proceed to next step B Temperature Sensor Failure e Temperature sensor is located on top of the unit close to exhaust airflow sensor and attached on the white metal box See Figure 5 3 Using multimeter check the temperature sensor s output voltage DC at channel AIN4 and J4 on main board See Figure 5 2 0 01VDC should represent one degree Celsius For example if output is 0 25VDC it indicates temperature of 25 C Tolerance of 1 C is acceptable Replace temperature sensor if the reading is wrong If temperature sensor is working properly proceed to next step Z nilevdsclinactior E Temperature Figure 5 1 Sensor Figure 5 2 E co Airstream Problem 6 Excessive Blower Noise Cause Corrective Action Resonance NOTE Requires a Certifier Locate the motor speed controller Measure the motor v
47. Thimble Exhaust Collar ULPA Filter Downflow gt 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 3 USA Efficiency Exhaust gt 99 999 at MPPS as per EN 1822 H 14 EU Sound NSF ANSI 49 lt 62 dBA lt 61 dBA lt 62 dBA lt 62 dBA lt 63 dBA Emission EN 12469 lt 59 dBA lt 58 dBA lt 59 dBA lt 59 dBA lt 60 dBA gt 900 Lux gt 1130 Lux gt 1280 Lux gt 1050 Lux gt 1220 Lux Fluorescent Lamp Intensity gt 84 foot gt 105 foot gt 119 foot gt 97 foot gt 113 foot candles candles candles candles candles 1 2 mm 0 05 18 gauge electro galvanized steel with white oven baked epoxy polyester Main Body S Cabinet Isocide antimicrobial powder coated finish Construction Work Zone 1 5 mm 0 06 16 gauge stainless steel type 304 with BA finish Side Walls UV absorbing tempered glass 5 mm 0 2 colorless and transparent 220 240V AC AC2 2E1 AC2 3E1 AC2 4E1 AC2 5E1 AC2 6E1 50Hz 1 60Hz 1 220 240V AC AC2 2E3 AC2 3E3 AC2 4E3 AC2 5E3 AC2 6E3 60Hz 1 Net Weight 160 kg 353 Ibs 177 kg 390 lbs 203 kg 447 lbs 251 kg 552 Ibs 299 kg 658 165 Sound emission test is conducted in an open field condition anechoic chamber er Cabinet only excluding retrofit kit amp support stand 28 Pa 0 11 in 29 0 11 31 0 12 in 35 0 14 in 47 0 18 H20 H20 H O H20 E Cco Airstream A 9 2 2 S Series 9 2 1 Engineering Drawing
48. be Fluorescent tubes are located inside the blue panel The white lamp holder can be pulled off from the tubes Defective Check voltage coming to the Relay board Fluorescent ballast Locate LS7 circuit and measure AC voltage between BLUE NEUTRAL terminal block or Relay Board and the end terminal with Red cable on the three terminal strip J13 on relay issues board If there is voltage to the end terminal Terminal with cable check the central terminal Normally Open Circuit to the BLUE NEUTRAL terminal block No voltage at the central terminal indicates a blown fuse or the circuit is not energized Check Fuse F7 on relay board See Figure 7 1 below If fuse F7 is blown as temporary solution use F5 spare to replace F7 If fuse F7 is okay check the voltage between the BLUE NEUTRAL terminal block and the end terminal on the LS7 circuit J13 on relay board Voltage at the end terminal with no cable indicates the F7 fuse is good and the circuit is not energized Make sure the LIGHT LED is on If the ballast has power and the bulbs are new the problem is either connections or the ballast Check Connector C connections If there are no lose terminals replace the ballast Class Biological Safety Cabinets Ecco X Black cable Terminal ballast input J13 Ni i 230 or 115Vac ay RELAY BOARD Figure 7 1 Ready magnetic With the LIGHT LED ON and the sash at the Normal operating position Ready switch Issues Does the
49. binets Ecco E co Airstream Chapter 9 Engineering Details 9 1 AC2 E Series 9 1 1 Engineering Drawing _ a B c D FE z 660mm 730mm 172mm 242mm 250mm 26 4 28 7 6 8 9 5 9 8 3 970mm 1035mm 235mm 360mm 352mm T ES ti 1 38 2 40 7 9 3 142 13 9 P f L 1270 1340mm 360mm 458mm i Waa Lu 50 0 52 7 9 3 142 180 NL LA 1 1570 1645mm 360 520 61 8 64 7 9 3 13 9 20 5 pE 1870 1950mm 235mm 360mm 975mm 73 6 76 7 9 3 14 2 38 4 250mm 9 8 C gt d NES i i 14 15 16 9 EE Aa 5Teee E 216 con 4 9 V a 17 10 ur 5 N 11 b o 6 O 12 MN 137 gt 550mm 21 7 732 28 8 800mm 31 5 1 Sensor Box 7 Exhaust Collar 12 Work Tray 2 RS232 Port Optional 13 Armrest 3 Power In
50. buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose AIRFLOW MONITOR Press SET button to confirm Use UP DOWN buttons to choose between ENABLED and DISABLED Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display A 2 2 4 4 Filter Life Display Using this option the user can select the whether the filter life is displayed or not Filter life is calculated based on the filter hour meter F H M The filter life display will count down according to the amount of hours left in the filter hour meter with respect to filter life expectancy of 10000 hours When the filter is changed the F H M must be reset please see section 2 2 4 7 to reset the F H M ENABLED MENU gt ADMIN SETTINGS m FILTER LIFE DISP gt DISABLED Class Biological Safety Cabinets ECCO To Set Filter Life Disp 1 CO a Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose FILTER LIFE DISP Press SET button to confirm Use UP DOWN buttons to choos
51. buttons to choose the hour HH Press SET button to confirm Do the same for the minute and second The display will Show DATA LOG PERIOD SET for a few second and then return to SETTING 8 Press MENU button twice to return to the main display guru m 2 2 2 Setting Mode AC2 BSC has three working mode two of which normal mode and quickstart mode can be used in daily activity Both of these modes be seen and accessed when you enter the FAN PIN NORMAL MODE MENU SET MODE gt QUICKSTART MODE MAINTENANCE MODE To Set the Mode 1 Press MENU button to enter the menu display if the BSC is secured by a FAN and ADMIN PIN then it will ask for the appropriate PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SET MODE Press SET button to confirm Use UP DOWN buttons to choose between NORMAL MODE QUICKSTART MODE and MAINTENANCE MODE Press SET button to confirm 6 Thedisplay will return to SET MODE 7 Press MENU button twice to return to the main display A 2 2 2 1 Normal Mode The normal mode is activated by factory default In this mode all alarms and interlocks are enabled 2 2 2 7 Quickstart Mode Quickstart mode allows the user to activate the blower and the light sim
52. cabinet is not excessively loaded If there is no blockage in air grilles proceed to next step Open electrical box and find motor voltage Test Point shown in Figures 4 2 4 3 NOTE A qualified Certifier should make all voltage adjustments For a new cabinet if the motor voltage is lower than the Nominal Voltage value recorded in the factory test report adjust the speed controller to get output voltage to blower as specified in the Test Report Test Conditions Documentation Sheet For cabinet that has been used for more than 1 year refer to last motor Nominal Voltage from last certification report or if not available measure the actual airflow on cabinet and increase motor voltage accordingly to achieve nominal airflow velocity If supply voltage is same as Nominal voltage proceed to next step Cabinet must be turned on to perform this test Locate the 5 pin connector On the BACK side of the 5 pin connector measure the DC voltage between Green pin 5 and Black pin 4 Measure on the side away from the SMPS See Figure 1 4 and 1 5 The voltage should be in range of 12VDC 10 You do not have to measure voltage on the other side of the 5 pin connect If out of range check incoming power to the SMPS Molded cord check terminal where the cord is connected The input should be 10 of the cabinet voltage If you have input voltage but no output voltage replace the SMPS Refer to the Test Report for this model and set the air flow values to the requ
53. ck magnetic UV interlock switch on Left side as you face the cabinet of sash and K2 relay Check the AC voltage to and from the K2 relay No voltage indicates a wiring issue between the RED Live power in and the K2 relay If voltage to the K2 relay is present but no voltage out of the K2 relay this indicates the relay is not energized or defective Check the DC voltage to the coil of the K2 relay Voltage should be 12VDC 1096 No voltage indicates the magnetic switch on the Left side is not closed Check the wiring to the switch Connector D 6 and D 3 To simulate a closed switch jumper between connector D 6 and D 3 at Connector D If the relay does not energize check the wiring from the K2 coil to Connector D and the neutral wiring from K2 to the DC Power supply If wiring connections are OK visually inspect K2 relay for any burnt or flash marks If burnt or flash marks are present replace the relay If no burnt or flash marks are found turn off the cabinet Disconnect relay cables and check for the continuity at K2 relay terminals See figure 8 2 NC to COM has continuity while NO to COM doesn t have continuity Replace the relay if any of the terminals are found with wrong continuity configuration TE e Munt I n s IT T SERESA Til Relay Figure 8 2 Class Biological Safety Cabinets Ecco UV magnetic Move sash to Fully Closed position The LCD should show SASH FULLY CLOSED switch is defective If t
54. d required Light Control Socket Control UV Control Interlocked Interlocked Interlocked Y ER or ADMIN PIN Y ER or ADMIN PIN MENU Access en De s Yes no PIN required READY required required Complete Diagnostic Complete Diagnostic Complete Diagnostic Pressing Set button Timer Current Time Display OEA OR IIS EE EEE Air Velocity Display rr E If not calibrated displaying Sensor Uncalibrated Air Velocity Display Status Yes 1 o Yes No isplay al Air Fail checking is ang if air fails Sash Position Sash Position Detection Current Time Display Sash Alarm Yes not mutable Yes not L Not EIER Yes without WARM UP amp Yes without WARM UP amp Yes without WARM UP amp Fan Control PURGING time no PIN PURGING time no PIN PURGING time no PIN SASH required required required Ar Velocity Display vs es _ Ar Velo Delay Salas e AFalcheckng Yes display alert ifair fails ooo Ye e PE 1 FULLY Yes with WARM UP amp Yes with WARM UP amp OPEN Fan Control PURGING time FAN PIN PURGING time FAN PIN Not Applicable required required SwmcOFFFanrequsted Ne uuy Fan Control interocked Yes A ES A NA E Class Biological Safety Ca
55. d sash window The motorized sash uses a push and hold mechanism so if you remove your finger from the button the sash will stop immediately this is a safety feature to control the closure and prevent anything getting trapped in the aperture as the sash descends Lower Sash from Fully Open Position When the sash is fully open pressing the down button and holding it will cause the sash to move to the Safe Height setting and stop If the fluorescent lights are on as the sash descends they will stay on as long as the sash stops in the Safe Position If you release the button before the sash has reached Safe Position the lights will switch off automatically Lower Sash from Safe Height Position When the sash is at safe operating height pressing the down button and holding it will cause the sash to move down to the fully closed position and stop If the fluorescent lights are on as the sash descends they will switch off automatically as soon as the sash reaches fully closed If you release the button before the sash has reached the fully closed position the lights will switch off automatically Raise Sash from Fully Closed Position When the sash is fully closed pressing the up button and holding it will prompt the user to input the password to turn on the fan If the password is correct if it was on fan will turn on and the sash will move up to the Safe Height setting and stop Raise Sash from Safe Height Position When the sash is safe o
56. e 3 3 Check the green plastic cable from the Interface board to the Display board Figure 3 4 ESCO Airstream Fluorescent NES 4 n Gable to 2295111 2 N holder Interf ce Board e gt 4 Metal 7 deflector Figure 3 1 mM Figure 3 2 D Triangle sign is corresponding to Pin 1 of the male connector FRC Flat Ribbon Cable EYPAD CON 2 5000lo Figure 3 3 A V Interface board olololo folo ola llolo Jo o Relay 155 Relay LSB BUZZER Relay En Rev X Trace face down Black color Relay Board EQR EL MC 02 Figure 3 4 Defective Cable e Replace them one by one to check which one s among them is are Defective and or Interface e Replace the Defective part s Board and or keypad Problem 4 Blower doesn t function Corrective Action Motor Blower Fan Switch on the Fan by pressing Fan button on control panel is not running Enter the Fan PIN number if required default is 0001 The LED for FAN should illuminate and the
57. e between ENABLED and DISABLED Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display 2 2 1 5 Language Using this option the user can select the language of messages displayed on LCD gt ENGLISH MENU gt ADMIN SETTINGS e SET LANGUAGE gt FRANCAISE p ESPA OL To Set Language 1 oy gt 8 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose SET LANGUAGE Press SET button to confirm Use UP DOWN buttons to choose between ENGLISH FRANCAISE and ESPANOL Press SET button to confirm The display will return to ADMIN SETTINGS Press MENU button twice to return to the main display 2 2 4 5 Reset B H M This option is used to reset the blower hour meter The blower hour meter indicates how long the blower has been in operation There s no maximum value in blower hour meter The counter value can be checked while in the diagnostic mode The value can also provide some help in setting up maintenance schedule MENU o ADMIN SETTINGS RESET B H M
58. e cleaning 3 2 Starting and Shutting Down the BSC 3 2 1 Turning on the BSC 1 Raisethe sash to the indicated normal operational height READY state The lamp will turned on when this height is reached Note When Quickstart mode is selected fan will turn on as well without pressing the fan button 2 Turn on the fan by pressing the FAN button Input the Fan PIN if asked if PIN 0000 This will start the warm up procedure default 3 minutes buttons are diabled during warm up period 3 BSC is ready for work 3 2 2 Turning off the BSC 1 Turn off the fan by pressing the FAN button Input the Fan PIN if asked if PIN 0000 This will start the post purge procedure default O minute All buttons are diabled during post purge period 2 Lowerthe sash to the fully closed position the display will Show UV MODE The sash can be lowered immediately after turning off the fan as it will not interrupt the post purge procedure Note When Quickstart mode is selected fan will turn off without pressing the fan button 3 Turn on the UV lamp when present to decontaminate the work area by pressing the UV button Leave the UV lamp on to make sure the decontamination is done effectively The UV lamp can only be turned on after the post purge procedure is finished 3 3 Working in the BSC e Surface decontaminate the work area work surface back and side wall UV lamp electrical outlet service fixture and the inner surface o
59. ed payment e Problems that result from o External causes such as accident abuse misuse problems with electrical power improper operating environmental conditions Servicing not authorized by Esco Usage that is not in accordance with product instructions Failure to follow the product instructions Failure to perform preventive maintenance Problems caused by using accessories parts or components not supplied by Esco Damage by fire floods or acts of God Customer modifications to the product O O O O 0 00 Factory installed customer specified equipment or accessories are warranted only to the extent guaranteed by the original manufacturer The customer agrees that in relation to these products purchased through Esco our limited warranty shall not apply and the original manufacturer s warranty shall be the sole warranty in respect of these products The customer shall utilize that warranty for the support of such products and in any event not look to Esco for such warranty support Esco encourages user to register product online at www escoglobal com warranty or complete the warranty registration form included with each product ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY NO WARRANTIES WHETHER EXPRESS OR IMPLIED WILL APPLY AFTER THE LIMITED WARRANTY PERIOD H
60. ediate mediate Parameters for use Concentration Contact time min Stability gt 1 week 1 o cesse ee eoe i Agents Bacterial Endospores A U ae se a an le Naked Viruses O Mycobacterium Vegetative Bacteria Enveloped Viruses Characteristics Inactivated by Organics Residual Corrosive Flammable Skin Irritant Eye Irritant Respiratory Irritant Toxic Use in BSCs Routine Surface Decon Biohazardous Spill 1 protected from light and air effective results may vary blank not effective Decontamination Decontamination may frequently be carried out by means of formaldehyde fumigation or using other decontamination agents such as chlorine dioxide or hydrogen peroxide Decontamination process should only be carried out by trained personnel In any of the following eventualities the user should ensure that the BSC has been properly decontaminated keeping in mind the nature of the pathogens used e Atthe time of moving relocating the BSC e time of changing the type of work being carried out in the BSC e Before accessing contaminated areas for servicing for example filter replacement Class Biological Safety Cabinets Ecco E co Airstream Chapter 4 Installation 4 1 General Requirement 4 1 1 Location Requirements Placing the BSC in a proper location is important Bad location may affect the performance of the cabinet Relative Air Velocities
61. er than formalin Being a true gas it spreads quickly without the need of pulsing the BSC s blower It can rapidly kill the micro organisms with high efficacy with just 1 hour contact time There is minimal residue to clean after the decontamination making the entire process much faster than formalin decontamination The time required for the entire process of chlorine dioxide decontamination is as follows No Process Time Set up amp sealing the BSC to make it air tight Chlorine dioxide gassing Chlorine dioxide contact time Chlorine dioxide scrubbing Tear down amp cleaning the minimal residue TOTAL Chlorine dioxide has the PEL of 0 1 ppm compared to 0 75 ppm for formalin In both processes airtight BSC sealing is required to protect personnel from the gas exposure 7 1 3 Hydrogen Peroxide Decontamination Hydrogen peroxide 202 decontamination is performed by flash vaporization of an aqueous peroxide mixture creating a vapor that is distributed throughout the inside the BSC STERIS and BIOQUELL are two major vendors of hydrogen peroxide generators There are significant differences in operating principles e The STERIS principle is to avoid condensation on surfaces to minimize corrosion and optimize vapor distribution The relative humidity inside the BSC must be lowered to 3096 so that the remaining 7096 relative humidity can be occupied by the hydrogen peroxide vapor STERIS product for BSC is VHP M100 Biodecontamination
62. ering the menu during this time will pause the warm up period MENU SETTINGS _____ WARMUPTIME gt XX MINUTES To Set Warm Up 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose WARM UP TIME Press SET button to confirm The time is set in MM format Use UP DOWN buttons to set the warm up period Press SET button to confirm 7 The display will show WARM UP SET for a few second and then return to SETTINGS 8 Press MENU button twice to return to the main display DE I EE 2 2 1 6 PostPurge Time After the user switches off the BSC blower there will be a post purge period to ensure that all contaminants are purged from the work zone The default setting is zero minute disable and user can set it between O to 15 minutes However it is recommended to purge the BSC for around 3 minutes after the work is complete MENU SETTINGS POSTPURGE TIME gt XX MINUTES To Set Post Purge 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for t
63. ertification amp Calibration BSC generally require re certification when e The cabinet is re located e Cabinet s performance is suspected e After filter or blower replacement e Atleast once a year During recertification e Cabinet airflow velocities and flow patterns are verified against the manufacturer s specifications and relevant international standards e Filters are scan tested to ensure they do not leak e Operator comfort tests may be performed e If airflow velocities are found to be off set point adjustments are made as part of the certification process before final values are recorded Airflow alarm calibration when e The cause of the airflow alarm cannot be determined e Re certification indicates the displayed airflow deviates by gt 0 02 m s 4 fom from actual measured velocities 6 1 Certification Flowchart Motor Filter Inflow Downflow Decontamination Voltage Leak gt Velocity Velocity Checking Test Microprocessor re calibration As needed Noise Smoke Pattern m Level cu Vibration Light Personnel Pressure Soap Protection Bubble Test Test Method For new cabinets commissioning For used contaminated cabinets Mandatory TTT _ _ _oao e Foco 5 A 6 2
64. es in order to achieve comfortable and healthy working conditions e Always ensure that your legs have enough legroom e Keep your lower back comfortably supported by your chair Adjust the chair or use a pillow behind your back whenever necessary e You should place your feet flat on the floor or on a footrest Don t dangle your feet and compress your thighs e You should keep varying your sitting position throughout the day at regular intervals so that you are never in the same posture for too long e Observe the following precautions with respect to your eyes o Give your eyes frequent breaks Periodically look away from the work area and focus at a distant point o Keep your glasses clean e Arrange the items apparatus frequently used in your work in such a way that you can minimize the physical strain involved in handling them e Exercise regularly The BSC s noise emission has been tested and found to be in compliance with EN 12469 ISO 4871 and NSF ANSI 49 which is important to ensure health and comfort for the operator Ergonomics accessories available with Esco include e Armrest padding e Lab chair e Footrest Please contact your local distributor or Esco for more information Class Biological Safety Cabinets Ecco 3 5 UV Lamps The UV light is a very effective germicide and virucide Even at the minimum acceptable irradiance in a BSC 40 uW cm US Department of Health and Human Services et al 2000 it would only take 12
65. escription hb Connection E ntar a name and choos anican tor the Hame LAJ 2010 40321 County regia Iron Asa code Phone number Connect using v 2 In the Name box and the Icon box 3 Choose the port in the Connect Using enter an appropriate name and icon drop down box e g COM1 E co Airstream COM1 Propertics Por Settings Function and chil act ac ASCII Setup Taminal keys Windows kaye Seed ine ende with Ing feeds Bis per recond 12 00 key senda O O Del O Spece Echo typed characters locally Data bite E A Line dea 0 miiseconos Emulation Parby uto detect Terminal Setup pe Telnet terminal ID AMS ASCII Receiving Stoo Backecrol bulfar lines 500 7 Append lire feeds lo ncoming ine ends C Fores incoming data to 7 bit ASCII 9 Wrap lines that be minal wicks Input Translation ASCI Sewn Lac Cancel anc Flaw control O Pia sound when connecting ar disconnecting Restore Defaults zn 4 Enter the connection s setting 5 Enter File Properties Setting ASCII Setup and tick Append line feeds to incoming ends 6 Click OK button twice to return to the HyperTerminal window The connection is configured successfully 7 Theinformation
66. etic Compatibility Directive and its amending directives has been designed to comply with the requirement of the following Harmonized Standard Low Voltage EN 61010 1 2001 EMC EN 61326 1 2006 Class B Design EN 12469 2000 Class Biological Safety Cabinet Performance Criteria More information may be obtained from Esco s authorized distributors located within the European Union A list of these parties and their contact information is available on request from Esco Lin Xiang Qian Vice President of Engineering Division This Declaration of Conformity is only applicable for 230V AC 50Hz units Class Il Biological Safety Cabinets Ecco ECCO Airstream Chapter 1 Product Information 1 1 About Airstream Biological Safety Cabinets BSCs The Esco Airstream Class Il Biological Safety Cabinet is a cost effective solution to providing operator product and environmental protection within laboratories and industrial facilities The Airstream features the use of 2 ULPA filters to deliver ISO Class 3 air cleanliness to the work zone The Esco Airstream Class Il Biological Safety Cabinet has been independently certified and type tested by Health Protection Agency HPA formerly CAMR the Centre for Applied Microbiological Research Porton Down Salisbury UK for compliance with the Class Il requirements of the EN12469 2000 European standard for microbiological safety cabinets The type testing performed included stringent eva
67. f the sash window before and after using the BSC e Allow the BSC to purge any contaminant by allowing the blower to operate at least 3 minutes before and after using the BSC e Surface decontaminate any item including your arms or apparatus with appropriate disinfectant before entering or exiting the work area e Place the waste container containment bag pippete discard pans etc inside the BSC work area e Ly 2 m and Tr the safe working area EY LOS LL M LA 4 EE m Area ub BSC 000 N 000 000000000000000000000000000 00000000000000000002000000 1 2 620 la Airstream N e Minimize room activity personnel movements closing and opening of doors etc since these external airflow disturbances may adversely affect the BSC s internal airflow thereby possibly impairing the containment capabilities of the BSC e Ensure that the sash is at normal operating height READY state before starting experiment e Ensure the front and back air grilles are not obstructed by your arms any other objects e Work as far inside the BSC as possible at least 150 mm 6 inches behind the front air intake grille e While working in the BSC move your hands slowly and in a controlled manner Rapid movements may disrupt the a
68. fety cabinet their discharge velocities and the force air handling rates will need to be low The position of a safety cabinet should satisfy the spatial requirements e g vision lighting and convenience of access of the operator and people working nearby If the cabinet is installed on a bench top the leading edge should slightly overhang or be flush with the edge of the bench top There should not be an open space between the leading edge of the cabinet and the front of the bench as this may create turbulence in front of the aperture It also provides an obstacle which could adversely affect airflow across the cabinet face 4 1 1 1 Position Requirements Allow at least 1 0 m 3 between the cabinet and pedestrian traffic routes thoroughfares or walkways SIT III o nun EEE ST x SSN SS SI py f Y Y M NS SSS MS IR SSS IS SI SSS E SSS SSS SS Z SS Ss N Uy Y fp yy Ss Yj 7 SEN SSS Ss IQ N gt III N IN N RR SS SS SSS SST SSS x SST SS M SS II SSS QS AA A gt H KH FH e N N SS SENS N SI x 30 cm 1 Y Allow at least 30 cm 1 clearance on both sides of the cabinet Class Biological Safety Cabinets Ecco 7 7 15 5
69. fterward e Clean the sash window using an appropriate disinfectant agent and glass cleaner afterward Useadamp cloth to clean the exterior surface of the BSC particularly on the front and top in order to remove dust that accumulated there e Use clean water to finish the cleaning and wash away any residue of disinfectant agent soap water and glass cleaner e For removing stubborn stains or spots on the stainless steel surface make use of Methyl Ethyl Ketone In such cases make sure that you wash the steel surface immediately afterwards with clean water and some liquid detergent Use a polyurethane cloth or sponge for washing Regularly cleaning the stainless steel surface can help you retain the attractive factory finish Test the audible and visual alarm The simplest method by far would be to move the sash until the glass window is no longer in the sash ready or UV mode position Check the cabinet s functionality e Check the BSC s mechanical functionality ex sash window lubricate if necessary e Check the BSC s electrical functionality ex fluorescent lamp replace if necessary e Check the BSC for any defect repair immediately Re certification All BSC must be re certified annually by a certified engineer See test report for recertification procedure Class Biological Safety Cabinets Ecco Accessing the paper catch The purpose of accessing the paper catch is to remove any retained materials that might cause ob
70. gauge stainless steel type 304 AC2 4D1 AC2 6D1 220 240V AC 220 240V AC 60Hz 1 AC2 4D3 AC2 6D3 Net Weight 223 5 kg 492 7 lbs 315 kg 694 165 Sound emission test is conducted in an open field condition anechoic chamber T Cabinet only excluding retrofit kit amp support stand ECCO Airstream 9 4 AC2 Series 9 4 1 Engineering Drawing 1470mm 57 9 1450mm 57 1 _ a B Y 1270mm 1340mm 1 50 0 52 7 a 1870mm 1950mm 2 13 6 a Ed y UEM 14 de 7 15 1 p 16 3 8 i d 17 12 550mm 21 7 732mm 28 8 800mm 31 5 670 26 4 1 RS232 Port 7 Electrical Panel 13 2 Exhaust ULPA 2 Power Inlet 8 Fluorescent Lamp s Filter 3 Sentinel Gold 9 IV Bar Retrofit Kit 14 1st Exhaust ULPA Controller Provision Filter 4 Sash Window 10 Plugged Service 15 Blower 5 Electrical Outlet Fixture Provision 16 Downflow ULPA Filter Retrofit Kit Provision 11 Work Tray 17 UV Lamp 6 Sensor Box 12 Armrest Class Biological Safety Cabinets Ecco 9 4 2 Genera
71. ge Yes due to leakage No people can still ager amen O Hi extensive cleaning of NaCl cleaning at all Decontamination time per BSC Equipment cost 055 100 2 000 USS 1 500 CI gas USS 18 000 5 52 000 reusable canister reusable for reusable several times 7 2 Recommended Decontamination Sealing Method EE AM 1 Putthe decontamination bag to enclose the 2 Usethe 50 mm wide aluminum tape to seal the entire BSC and support stand gap between the decontamination bag and the floor To compensate for areas where the decontamination bag must turn use multi section tape Ensure that the two layers of tape overlap Class Biological Safety Cabinets Ecco at the end points to prevent leakage nm 3 Overlap the tape at the joints 4 These 2 cables for BSC and for vaporizer should be combined so that only 1 cable should come out from the decontamination bag to minimize chance of formalin leakage 2 5 To seal the cable first make O shape 6 Put the bottom part of the decontamination bag aluminum tape around the cable and make a 5 on top of the aluminum tape and then seal it cm flat portion going to both directions Then with another layer of aluminum tape Use paste the aluminum tape to the floor multiple layer of aluminum tape at least 2 layers on both directions to ensure leak tightness 7 The BSC is ready for formalin decontamination E co Airs
72. he LCD display shows SASH NO the sash is not fully closed or misaligned Check the magnetic switches There are 3 magnetic switches inside the right profile See Figure 7 2 above The bottom one is for UV Mode position There is a magnet attached to the glass beside the bottom switch Shift the switch or magnet position so the distance between them is between 10 13 mm 3 8 to 1 2 as explained in Figure 7 2 above If the distance is too far the switch may not be able to detect the magnet If the LCD still showing SASH NO check the connections at Connector D pins D 4 and D 5 To simulate a closed U V Switch jumper between Connector D pins D 5 and D 4 If this does not turn on the UV go back to the start of this procedure and check it again Corrective Action Connection Is the OUTLET LED Illuminated If not press the Outlet button at the keypad Problem membrane in front panel Check voltage coming to the Relay board Locate LS4 J6 Terminal circuit and measure AC voltage between Large BLUE Neutral terminal block and the end terminal with Red cable COM on the three terminal strip J6 If there is voltage to the end terminal with Red cable COM check the central terminal Normally Open circuit to the BLUE Neutral terminal block No voltage at the central terminal Normally Open circuit indicates a blown fuse or the circuit is not energized Check Fuse F4 on relay board See Figure 9 1 below If fuse F4 is blown replace with F5 fuse
73. he PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose POSTPURGE TIME Press SET button to confirm The time is set in MM format Use UP DOWN buttons to set the post purge period Press SET button to confirm E co Airstream O 7 The display will show POST PURGE SET for a few second and then return to SETTING 8 Press MENU button twice to return to the main display 2 2 1 7 Data Log Period Using RS232 communication port the BSC can send data on the condition of the BSC to a PC Data Log Period option allows the user to control the amount of time in between the BSC data sending procedure For more information on the data logging feature of this BSC please check section Connecting the BSC to PC SETTINGS DATA LOG PERIOD HH MM SS To Set Data Log Period 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose DATA LOG PERIOD Press SET button to confirm The time is set in 55 format Use UP DOWN
74. he alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose SET TIME Press SET button to confirm The time is set in HH MM format Use UP DOWN buttons to choose the hour HH Press SET button to confirm Do the same for the minute The display will show TIME SET for a few second and then return to SETTINGS 8 Press MENU button twice to return to the main display QU cer 2 2 1 2 UV Timer UV timer can be used to switch off the UV lamp automatically after a fixed period The UV timer can be set up to 18 hours By default the timer is set to 60 minutes which is considered the most effective UV decontamination time Esco does not recommend leaving the UV lamp on for more than 60 minutes as it shortens the lifespan of the UV lamp Without the UV timer the lamp has to be switched off manually MENU gt SETTINGS 5 UV TIMER gt HH MM To Set UV Timer 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose UV TIMER Press SET button to confirm The time is set in HH MM format Use UP DOWN buttons to choose the hour HH Press SET button to co
75. in presents the following health risks e External contact can cause irritation to skin eyes and mucous membranes e Inhalation in small concentrations can cause coughing nausea and diarrhea e Inhalation in large concentrations can cause convulsions coma and death e Long term exposure can cause cancer Although the Permissible Exposure Level PEL for formalin is 0 75 ppm many scientists believe that there is no safe level of carcinogen exposure to humans Therefore typically the room must be evacuated when the decontamination process is performed which leads to lab down time The use of formalin decontamination also has other disadvantages e process is time consuming e The certifier needs to pulse the BSC fan to circulate the formalin vapor This can dislodge the tape holding the plastic sheet covering the exhaust filter e Due to excessive residue extensive cleaning must be done after decontamination and before use The formalin decontamination process can require an extended period of time as outlined below According to OSHA Occupational Safety and Health Administration USA formaldehyde Short Term Exposure Level STEL is 2 ppm for 15 minutes exposure 4 times a day minimum of 60 minutes in between exposures Any additional local safety regulations should also be observed Personnel should be given adequate training The following links provide general guidelines on formaldehyde safety e Regulations Standards 29 CFR
76. ir barrier allowing contaminants to escape or enter the BSC e The use of Bunsen burner inside the work zone is not recommended However if the use of Bunsen burner is unavoidable place the burner in the right side of the work zone e Place aerosol generating instruments as far inside the BSC as possible and at least 150 mm 6 inches from clean items materials e Place air turbulence generating equipment such as centrifuges blenders or sonicators towards the back of the BSC Stop other work while any of this equipment is in operation e As far as possible it is recommended that the BSC be operated continuously in order to achieve optimal containment and cleanliness Airflow studies have shown that once the fan has been switched off air from the BSC may escape due to the thermal currents from inside the BSC 3 4 Working Ergonomics On most occasions you would most likely be operating the BSC in sitting rather than standing posture There are some obvious advantages of the sitting posture e physiological energy cost and fatigue involved in sitting are relatively less e Sitting posture provides the body with a stable support However sitting position has some drawbacks too e The working area available is fairly limited e There is a potential risk of being constrained in the same posture for a long time e Sitting posture is one of the most stressful postures for one s back Therefore you should pay careful attention to the following guidelin
77. ired values This information is also contained on the label at the bottom of the BLUE front panel on the Left side as you face the cabinet Plug in the voltmeter to the Motor Voltage Sampling Port Measure the actual airflow velocity using anemometer or flow hood If actual airflow is okay but LCD still shows AIRFAIL proceed to next step If actual airflow is not okay please adjust motor speed controller accordingly until alarm is turned off and the LCD shows nominal airflow reading After this if LCD still shows AIRFAIL proceed to next step The sensor will take about 2 3 minutes before it can register a new airflow setting Note cabinet must be certified at least annually If the blower is already operating at maximum voltage and the airflow alarm is still triggered due to filter loaded it s time to change the filter If filter is not loaded proceed to next step E co Airstream Faulty inaccurate e Re calibrate the microprocessor Follow the calibration procedure in the test report calibration Warning To be carried out only by an authorized person CHECKING CALIBRATION DATA e After calibration is done go to main display by pressing MENU button twice e Press SET Diagnostic button LCD will display the mode and software version that is currently being used on the cabinet Example DIAGNOSTIC MODE NORMAL CP104D VERSION 1 5 Press DOWN button until you find the following TEMPERATURE Shows actual ambient temperature
78. is mode allows you to lower the sash further than usual 3 Lower the sash by pressing the DOWN button continuously until the sash is in the lowest position and create a 10 cm opening between the sash and the diffuser 4 Open the front panel 5 Slide your hand through the opening and clean the inner side of the sash using cloth and IPA 70 6 After cleaning push the UP button continuously until the sash is in the sash ready position 7 Close the front panel 8 Reattach armrest 9 Set the cabinet back to NORMAL MODE 5 2 Parts Replacement The use of non Esco parts and or parts not supplied directly by Esco or our authorized distributors including but not limited to maintenance parts spare parts replacement parts system components and or system accessories shall void all expressed or implied warranties 5 2 1 Filter Replacement Procedure e The filters should not be replaced until the entire cabinet has been decontaminated e Two persons may be required to facilitate filter changing on 5 and 6 BSC e The two ULPA filters under normal usage and barring an accident a puncture do not need replacement until the inflow and or downflow velocity cannot be maintained at the specific rate as mentioned in the test report even though the fan has been set to maximum speed e The cabinet must undergo re certification after changing filter D O a 9 5 3 Q 69 2 64
79. l Specification ___ 2 46_ 2 66_ External Dimensions 1340 x 800 x 1470 mm 1950 x 800 x 1470 mm WxDxH 52 7 x 31 5 x 57 9 76 7 x 31 5 x 57 9 Gross Internal Dimensions 1270 x 545 x 670 mm 1870 x 545 x670 mm WxDxH 50 0 x 21 5 x 26 4 73 6 x 21 5 x 26 4 Usable Work Area Avg Airflow Velocity 0 30 m s 60 fpm at initial setpoint with uniformity of better than 20 3 3 LE 3 741 m h 443 cfm 1091 m h 652 cfm Exhaust 356 213 cfm 524 313 cfm Required Exhaust Airflow Volume with Optional Thimble Exhaust Collar Static Pressure For Optional Thimble 31 Pa 0 12 in H O 47 Pa 0 18 in HO Exhaust Collar ULPA Filter gt 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 3 USA Efficiency Exhaust 0 gt 99 999 at MPPS as per EN 1822 H 14 EU Sound NSF ANSI 49 lt 62 dBA lt 63 dBA Emission lt 59 dBA lt 60 dBA Fluorescent Lamp Intensity gt 1280 Lux gt 119 foot candles gt 1220 Lux gt 113 foot candles Cabinet Main Body 1 2 mm 0 05 18 gauge electro galvanized steel with white oven baked epoxy polyester Construction Isocide antimicrobial powder coated finish 538 m h 317 cfm 823 m h 485 cfm Work Zone 1 5 mm 0 06 16 gauge stainless steel type 304 with 4B finish Side Walls UV absorbing tempered glass 5 mm 0 2 colorless and transparent eae Lk a AC2 4G1 AC2 6G1 Electrical 2 1 220 240 60Hz 1 AC2 4G3 AC2 6G3
80. let 8 Electrical Panel 14 Exhaust ULPA Filter 4 Sentinel Gold 9 Fluorescent Lamp s 15 Blower Controller 10 IV Bar Retrofit Kit 16 Downflow ULPA Filter 5 Sash Window Provision 17 UV Lamp 6 Electrical Outlet 11 Plugged Service Retrofit Kit Provision Fixture Provision Class Biological Safety Cabinets Ecco 9 1 2 General Specification A gt AC2 AE_ AC2 6E_ 730 x 800 x 1400 1035 x 800 x 1340 x 800 x 1645 x 800 x 1950 x 800 x External Dimensions mm 1400 mm 1400 mm 1400 mm 1400 mm WxDxH 28 7 x 31 5 x 40 7 x 31 5 x 52 7 x 31 5 x 64 7 x 31 5 x 76 7 x 31 5 x 55 1 55 1 55 1 55 1 55 1 660 560 670 970 x 550x 670 1270x550x670 1570x550x670 1870x 550x 670 Gross Internal Dimensions mm mm mm mm mm WxDxH 26 4 x 22 0 x 38 2 x 21 7 x 50 0 x 21 7 x 61 8 x 21 7 x 73 6 x 21 7 x 26 4 26 4 26 4 26 4 26 4 Usable Work Area 0 85 m 9 1 ft Avg Airflow Inflow 0 45 m s 90 fpm at initial setpoint Velocity Downflow 0 30 m s 60 fpm at initial setpoint with uniformity of better than 20 111 cfm 162 cfm 213 cfm 263 cfm 313 cfm Downflow 230 cfm 337 cfm 443 cfm 548 cfm 652 cfm Airflow 111 162 cfm 213 cfm 263 cfm 313 cfm Volume Required Exhaust With Optional 260 m h 320 m h 538 m h 615 m h 823 m h Thimble Exhaust 153 cfm 189 cfm 317 cfm 362 cfm 485 cfm Collar Static Pressure For Optional
81. locking arms The arms should be pointed in the same direction as the cable See Figure 2 1 below If the LCD Display is not on check power to main board as detailed in Problem 1 Figure 2 1 Adjust the potentiometer on main board by rotating the top metal part using flat screw driver to achieve the best LCD contrast Counter clock wise direction will increase the contrast See Figure 2 2 If the LCD remains blank replace it 4 va JI 44 MA N BOARD r EC y fec e lt y Pi Ps We 4 ST FEA TO gt AR Figure 7 2 Connect a new LCD to the LCD port on main board If the new LCD functions properly this indicates the old one is Defective If the new LCD does not work replace the large Flat Ribbon Cable If the LCD still does not work replace the main board Corrective Action Connection Does the LCD display work If not go to Problem 1 and make sure the main board problem has power Ensure that the small Flat Ribbon Cable is connected to the main board See figure 3 1 The triangle sign on the female connector of the cable indicates PIN number 1 Interface board and membrane keypad are located behind the blue panel underneath the metal light deflector Turn off the cabinet remove the bulbs and the clips that hold the bulbs Bulb connections pull off See Figure 3 2 Check connections between the cable from the main board and the Interface board See Figur
82. luations of cabinet airflows containment and operator comfort factors 1 2 Labels Electrical Requirement CABINET POWER INLET 220 240VAC 50Hz 7A single phase Damage may result if the cabinet is connected to an incorrect power source ECCO Model AC2 4E1 Serial 2010 53057 21 Changi South Street 1 singapore 48677 Singapore 486777 Power 360W 220 240VAC 50HZ 1PH Manufactured in 2010 Model The model of the unit Serial The serial number of the unit Power The power consumption and electrical requirement of the unit Manufactured year The year of manufacture Airflow Information t Cabinet Airflow Setpoin 5 AC24E1 Class ll BSC per EN 12469 2000 INFLOW 99 6Liter s DIM or 0 45 0 025m s Secondary method Grid 76mm opening 9 readings 127mm apart DOWNFLOW 0 27 0 36 5 12 points total Grid L R 159mm from wall amp 318mm apart F B 138mm from wall amp 138mm apart Safe work area non perforated flat portion of work tray Class Biological Safety Cabinets Ecco 1 3 Quick View 1 3 1 2 E amp AC2 _G_ Series Quick View 1 EscoSentinel Control System 5 Interior Work Area 2 Sash Window 6 Armrest 3 Service Fixture Provision optional 7 IV Bar Provision optional 4 Electrical Outlet Provision optional 8 Glass Side Wall E co Airstream 1 3 2 AC2 S and AC2 D Series Quick View 1 EscoSentinel Contr
83. n metric m s and imperial fpm gt METRIC MENU SETTINGS gt MEASUREMENT UNIT IMPERIAL To Set Measurement Unit 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose SETTINGS Press SET button to confirm Use UP DOWN buttons to choose MEASUREMENT UNIT Press SET button to confirm Use UP DOWN buttons to choose between METRIC and IMPERIAL Press SET button to confirm The display will return to SETTINGS Press MENU button twice to return to the main display 99 2 en MT 2 2 1 5 Warm Up Time There will be a period of warm up before the fan is fully functioning This is to ensure that the sensors the blower and the control system are stabilized as well as purging the work zone of contaminants The default setting is 3 minutes and the user can set it between 3 to 15 minutes During the warm up period the user can use the FAN button to turn off the blower LIGHT button to turn on and off the fluorescent lamp and MENU button However to be able to access the menu the user need to input ADMIN PIN and even then some portions of the menu WARM UP and all FIELD CALIBRATION is still not accessible for the user Ent
84. ncluding the area under the work surface Wipe down everything that is returned to the BSC If the contamination problem continues have the cabinet certified including air flows and filter leak tests Error Code Corrective Action Sash Error Position e Magnetic switches are located inside right profile cover See drawing B 1 below Switch 1 i E Shorted Switch_2 do uf amp Switch 3 Class Biological Safety Cabinets Ecco Magnet Mag Switch 1 Sash Window front view Mag Switch 2 Mag Switch 4 Mag Switch 3 N Sash movement range Magnetic Magnetic Magnetic Magnetic Switch 1 Switch 2 Switch 3 Switch 4 Sash is fully opened ALARM is given ALARM can be muted Cabinet sash is at nominal height ready to use Sash is fully closed UV can be operated SASH is at unsafe state ALARM is given ALARM cannot be Open Open Open Open SASH ERROR POSITION detected Description Table 2 SENSOR e Calibrate the controller Refer to test report to calibrate UNCALIBRATED E co Airstream Normal Or Quickstart Normal Or Quickstart Function Available Mode and Airfail Alarm Mode and Airfail Alarm Maintenance Mode Off Yes with WARM UP amp Yes with WARM UP amp Yes without WARM UP amp Fan Control PURGING time FAN PIN PURGING time FAN PIN PURGING time no PIN required require
85. ncrease and decrease corresponding value inside one of the menu options o Move the sash window upward and downward for motorized sash BSC o Accessing the stopwatch and experiment timer function for non motorized sash BSC 6 Set or Mute Button o Choose the menu or sub menu currently displayed on the LCD screen o Proceed to the next step or sequence inside one of the menu options mute the alarm sound only in fully open state during normal mode 7 Menu Button When you are entering menu options the alarm will sound to indicate that the microprocessor is not monitoring the operation of the cabinet No further warnings will be given o Toenter and exit from the menu options o back to the previous level of the menu options o Toaccess maintenance mode from ERR MSWITCH and AIRFAIL error condition Class Biological Safety Cabinets Ecco 2 2 Menu Options Please refer to the following diagram for complete reference to all menu options available SET TIME UV EXPERIMENT TIMER 55 SETTINGS MEASUREMENT UNIT METRIC IMPERIAL WARM UP TIME XX MINUTES POSTPURGE TIME XX MINUTES DATA LOG PERIOD 55 NORMAL MODE SET MODE MENU QUICKSTART MODE
86. nes at a higher velocity small purple arrows e Acombination of inflow downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone e Air returns to the common air plenum where the 3296 exhaust and 68 recirculation process is continued e Optimized air curtain maintains personnel and product protection even in the unlikely event of inflow and downflow imbalance that would compromise protection on a conventional cabinet Class Biological Safety Cabinets Ecco E co Airstream Chapter 2 Sentinel Control System 2 1 Sentinel Control System LCD Menu Up Fan Socket Display Button Button Button Button ECCO Sentinel GOL MICROFROCESSOR CONTROL SYSTEM Visual Set Mute Down Lamp UV Alarm Button Button Button Button 1 Fan Button o Turns on and turn off the fan 2 Lamp Button o Turns on and turn off the fluorescent lamps 3 Socket Button o Turnson and turn off the electrical socket retrofit kit The maximum rating of all the outlets in the cabinet is 5 A If overload the fuse will blow 4 UV Button o Turns on and turn off the UV lamp o UV lamp can only be activated when the sash window is fully closed Since the sash is capable of filtering UV rays users are protected from the harmful UV radiation 5 Up A and Down V Arrow Button o Move the menu options upwards downwards o I
87. nfirm Do the same for the minute The display will show UV TIMER SET for a few second and then return to SETTINGS 8 Press MENU button twice to return to the main display N 2 2 2 1 3 Experiment Timer Experiment timer is a countdown timer that can be used for critical experiment Experiment timer can be set between 00 00 00 and 17 59 59 MENU r SETTINGS gt EXPERIMENT TIMER gt HH MM SS To Set Experiment Timer 1 Press MENU button to enter the menu display if the BSC is secured by a FAN PIN then it will ask for the PIN otherwise go to step 3 2 Use UP DOWN button to enter the FAN PIN or ADMIN PIN digit by digit Press SET button to confirm 3 The alarm buzzer will sound 4 Use UP DOWN buttons to choose SETTINGS Press SET button to confirm 5 Use UP DOWN buttons to choose EXPERIMENT TIMER Press SET button to confirm 6 Thetime is set in HH MM SS format Use UP DOWN buttons to choose the hour HH Press SET button to confirm Do the same for the minute and second 7 The display will show EXPERIMENT TIMER SET for a few second and then return to SETTINGS 8 Press MENU button twice to return to the main display Class Biological Safety Cabinets Ecco 2 2 1 4 Measurement Unit Using this option the user can select the unit in which air velocity is measured and displayed The user can choose betwee
88. ning of this section to locate the speed controller This is indicated by the N1 and N2 D G series models only in the layout If you followed the steps outlined above and still the blower doesn t work Switch off the cabinet Bypass the speed controllers by adding jumper cable across two cables going to Niand 2 D G series models only See Figure 4 8 below Switch on the cabinet The speed controller is defective when the fan operates properly Replace the defective speed controller If the fan does not operate proceed to next step Speed control 706 123SB short cable connector To other circuits Figure 4 6 Figure 4 8 Defective Switch the cabinet off capacitor Locate the capacitor and disconnect two cables from the capacitor Refer to Figure 4 9 Using a capacitance meter or aDVM set to capacitance measurement Record the value between two capacitor cables If the value is out of given ranges on table 1 replace capacitor If the capacitance is within the given ranges proceed to next step Warning The capacitor may still have some electrical charges if the cabinet was on prior to performing this check Wait for 1 2 minutes and check the capacitor voltage using a voltmeter to ensure the capacitor is discharged voltage less than 5VAC prior to disconnecting the capacitor Disconnect 2 cables censum Capacitor1 Capacitor 2 AC2 _ESDG1 3 7 5 8 5uF 3 5 4 5uF 9 11uF AC2 2E2 AC
89. ntraller Class Biological Safety Cabinets Ecco Problem 1 Cabinet does not start LCD button fan light and socket inoperative Cause Corrective Action Power Failure e Check wall outlet for power e outlet has a switch make sure it is ON Power cord is not e Ensure the power cord is connected correctly to the wall outlet connected properly NOTE Some cabinets have 2 cords or faulty Disconnect the cord at the cabinet and measure the AC voltage between the live and the neutral terminal of the cord e If the voltage is not within 2 of the wall socket voltage replace cord otherwise proceed to next step Circuit breaker has e Check the circuit breaker inside the electrical panel tripped NOTE f circuit breaker has tripped do not reset the breaker before checking all electrical components and wiring connections See Figure 1 1 e Does the cabinet operate correctly after resetting the circuit breaker If not proceed to next step breaker Circut breaker normal condition ripped cond ion Figure 1 1 Improper Measure AC voltage between LIVE RED and NEUTRAL BLUE terminal blocks inside connection electrical box See Figure 1 2 Voltage must be within 1096 of the rated voltage Refer to cabinet Serial Number Tag for required voltage If the voltage is out of the range or there is no voltage check cable connection on both sides of connector A See Figure 1 3 230 VAC at Connector A pin 1 and
90. o choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose NEW FAN PIN Press SET button to confirm The FAN PIN consists of 4 numerical digits XXXX that the user must enter one by one Use UP DOWN buttons to choose the first digit X Press SET button to confirm Do the same for the following 3 digits 7 display will show CONFIRM PIN Press SET button to confirm 8 The display will return to ADMIN SETTINGS 9 Press MENU button twice to return to the main display E Tu 2 2 4 3 Airflow Monitor Whenever the air velocity falls below the fail point the air fail alarm will be triggered This option is used to enable disable alarm The alarm is enabled by default When the Airflow Monitor is disabled the warm up period is removed But the airflow will not be displayed for the first three minutes If the ambient temperature is outside of 18 30 which is the cabinet working temperature the Airflow Monitor is automatically disabled gt ENABLED MENU gt ADMIN SETTINGS m AIRFLOW MONITOR gt DISABLED To Set A F Monitor 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN
91. ol System 4 Interior Work Area 2 Sash Window 5 Armrest 3 Electrical Outlet Provision optional 6 Service Fixture Provision optional Class Biological Safety Cabinets ECCO C 1 4 Airflow Pattern 1 4 1 AC2 E and AC2 S Series Airflow Pattern ULPA Filtered Air Unfiltered Potentially contaminated Air Room Inflow Air e Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum fan plenum at the top of the cabinet Theuniform non turbulent air stream protects against cross contamination within and throughout the work area e Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small purple arrows Acombination of inflow downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone e Air returns to the common air plenum where the 3296 exhaust and 68 recirculation process is continued e Optimized air curtain maintains personnel and product protec
92. oltage and record this number Increase the motor speed by 5 10 VAC Does the noise change or go away If the noise is gone measure the actual airflow velocity Requires a Certifier o Ifthe velocity is still in the acceptable range maintain it o If velocity is out of acceptable range or if noise persists go to next step Loose motor or Note Decontaminate cabinet first before accessing the blower impeller wheel e Open the blower access panel and check if the blower mounting bolts are tight mounting e Doesthe motor rotate freely without noise e f motor is physically damaged replace it Problem 7 Light always OFF Corrective Action Sash in SASH Is the Light LED on the front panel ON ALARM state If not press the Light button and make sure the sash is in the Normal operating position Ready Position Normal Operating height Note The sash must be at the Normal Operating position Is the cabinet in Warm Up Note The fluorescent light will not light if the cabinet is in Warm Up unless the cabinet is in Quick Start Mode Move the sash to Ready position normal operating height Switch on the light by pressing LIGHT button on the membrane If the LED is ON the keypad and main board are functioning correctly If fluorescent light does not light with the window in the proper position proceed to next step If the LIGHT LED still does not light skip to Ready magnetic switch Issues Burned out Replace the faulty fluorescent tube fluorescent tu
93. peration position pressing the up button and holding it will cause the sash to move up to the fully open position and stop If the fluorescent lights are on as the sash rises they will stay on as long as the sash is allowed to fully open Stopping the sash midway will cause the lights to switch off automatically Class Biological Safety Cabinets ECCO 3 1 3 Using Sash Window e sash window should be fully closed when the cabinet is not in use This helps keep the work zone interior clean e sash window should always be in the normal operating height at all times when the cabinet is in use Even if the cabinet is left unattended but the blower is on the sash window should never be moved from the normal operating height unless during loading or unloading of materials apparatus into the cabinet e The alarm will be activated whenever the sash window is moved from the normal operating height e Whenever the sash window is moved to the correct height from a higher or lower position the light will automatically be turned on as a signal to the user e sash window may be opened to its maximum position for the purpose of loading unloading of materials apparatus into the cabinet When the sash window is fully opened the alarm sound may be muted by pressing MUTE button but will be automatically sounded again after 5 minutes to remind the user that it is not safe to work in the cabinet and the light will be turned on to facilitat
94. ply by opening the sash window to the operating position In this mode all alarms and interlocks are enabled 2 2 2 3 Maintenance Mode Maintenance mode should only be accessed by qualified personnel during maintenance In this mode all alarms are disabled and all interlocks are defeated Class Biological Safety Cabinets Ecco 2 2 3 Field Calibration The purpose of calibration is to ensure the accuracy of the airflow display and alarm if present This involves measuring airflow with reference instrumentation and establishing reference between airflow sensor s on the BSC to the standard reference Calibration should only be carried out by trained personnel This section is presents a brief overview of the calibration menu function For more information refer to test report gt AIRFLOW CALIB MENU gt FIELD CALIBRATION RESET CALIBRATION 2 2 3 1 Airflow Calibration This option allows proper calibration and operation of the airflow sensor alarm There will be three points to be calibrated namely inflow fail point inflow nominal point and downflow nominal point 2 2 3 2 Reset Calibration This option allows the user to reset all value calibrated in the field and return it to the values gathered in factory calibration 2 2 4 Admin Settings The admin menu allows you to change both FAN and ADMIN PIN The reset blower filter and UV hour meter functions a
95. re usually used after you change the blower filter or UV lamp as they can easily give you the indication on when to do BSC maintenance While the reset default function will return the options in the settings menu to their factory settings 2 2 4 1 New ADMIN PIN default 0009 ADMIN PIN restricts access to some of the more delicate menu functions namely admin and field calibration which should only be accessed by a qualified personnel User must enter four digits ADMIN PIN before accessing these menus ADMIN PIN can also be used to switch to maintenance mode from ERR MSWITCH and AIR FAIL error condition MENU p ADMINSETTINGS gt NEW ADMIN PIN LL XXXX To Set New ADMIN PIN 1 Press MENU button to enter the menu display if the BSC is secured by an ADMIN PIN then it will ask for the PIN otherwise go to step 3 Use UP DOWN button to enter the ADMIN PIN digit by digit Press SET button to confirm The alarm buzzer will sound Use UP DOWN buttons to choose ADMIN SETTINGS Press SET button to confirm Use UP DOWN buttons to choose NEW ADMIN PIN Press SET button to confirm The FAN PIN consists of 4 numerical digits XXXX that the user must enter one by one Use UP DOWN buttons to choose the first digit X Press SET button to confirm Do the same for the following 3 digits 7 The display will show CONFIRM PIN Press SET button to confirm 8 The display will return to ADMIN SETTINGS 9 Press MENU button twice
96. rical Panel Retrofit Kit Provision 16 UV Lamp Class Biological Safety Cabinets Ecco 9 3 2 General Specification ee AC2 4D AC2 6D_ External Dimensions 1340 x 800 x 1470 mm 1950 x 800 x 1470 mm WxDxH 52 7 x 31 5 x 57 9 76 7 x 31 5 x 57 9 Gross Internal Dimensions 1270 x 545 x 670 mm 1870 x 545 x670 mm WxDxH 50 0 x 21 5 x 26 4 73 6 x 21 5 x 26 4 Usable Work Area 0 56 6 0 sq ft 0 85 9 1 sq ft Avg Airflow Velocity 0 30 m s 60 fpm at initial setpoint with uniformity of better than 20 3 3 PE mov 3 741 m h 443 cfm 1091 m h 652 cfm Exhaust 356 m h 213 cfm 524 m 313 cfm Required Exhaust Airflow Volume with Optional Thimble Exhaust Collar Static Pressure For Optional Thimble 31 Pa 0 12 in H O 47 Pa 0 18 in H O Exhaust Collar ULPA Filter gt 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 3 USA Efficiency gt 99 999 at MPPS as per EN 1822 H 14 EU Sound NSF ANSI 49 lt 62 5 dBA lt 63 dBA Emission 12469 lt 59 5 lt 60 Fluorescent Lamp Intensity gt 1200 Lux gt 111 5 foot candles gt 1020 Lux gt 95 foot candles Cabinet Main Body 1 2 mm 0 05 18 gauge electro galvanized steel with white oven baked epoxy polyester Construction 538 m h 317 cfm 823 m h 485 cfm Isocide antimicrobial powder coated finish 1 5 mm 0 06 16 gauge stainless steel type 304 with 4B finish 0 9 mm 0 035 20
97. rposes only The contents and the product described in this manual including any appendix addendum attachment or inclusion are subject to change without notice Esco makes no representations or warranties as to the accuracy of the information contained in this manual In no event shall Esco be held liable for any damages direct or consequential arising out of or related to the use of this manual um Table of Contents INTRODUCTORY PAGES Table of Contents Warranty Terms and Conditions Introduction 1 Products Covered V 2 Safety Warning V 3 Limitation of Liability vi 4 European Union Directives on WEEE and RoHS vii Declaration of Conformity USER SECTION 1 1 1 About Airstream Biological Safety Cabinets 1 1 2 Labels 2 1 3 Quick View 2 1 3 1 AC2 _E_ amp AC2 G Series Quick View 3 1 3 2 2 5 amp AC2 D Series Quick View 4 1 4 Airflow Pattern 4 1 4 1 2 E amp AC2 5 Series Airflow Pattern 5 1 4 2 AC2 D amp 2 G Series Airflow Pattern 7 Chapter 2 Control System 7 2 1 Sentinel Control System 8 2 2 Menu Options 8 2 2 1 Settings 11 2 2 2 Setting Mode 12 2 2 3 Field Calibration 12 2 2 4 Admin Settings 16 2 3 Stopwatch and Experiment Timer 16 2 4 Alarm and Warnings 17 2 5 Diagnostic Mode Chapter 3 Basic Cabinet Operation 3 1 Sash Window Operation 5 3 1 1 Sash Window State 19 3 1 2 Operating Motorized Sash Window 20 3 1 3 Using Sash Window 20 3 2 Starting and Shutting Down the BSC
98. structions Care must be taken as the area is contaminated Before opening the paper catch e Use appropriate personal protective equipment PPE e Wipe down the interior of the BSC including the top and bottom surfaces of the work tray s while airflow is operating e If the interior cannot be wiped down the BSC should be decontaminated Accessing the paper catch E 1 Turn the blower off Then raise the sash to fully open position for easy access Remove the tray s to access the paper catch The figure beside points to the location of the paper catch Locate and remove any material trapped Be careful of any sharp objects that might be present You might want to use a mirror to help you see better Diffuser Sash Window 1 Stopper Armrest 1 Remove armrest 2 Lowerthe sash by continuously until the sash is in the lowest position and create a 10 cm opening between the sash and the diffuser Open the front panel Slide your hand through the opening and clean the inner side of the sash using cloth and IPA 7096 After cleaning push continuously until the sash is in the sash ready position Close the front panel Reattach armrest A E co Airstream Sash cleaning procedure for motorized sash BSC 1 Remove armrest 2 Press MENU button select SET MODE then Front Panel __ N choose MAINTENANCE MODE th
99. t timer function and reset the timer The timer in the experiment timer function is counting down and shown using the HH MM SS format Operator can use the SETTINGS EXPERIMENT TIMER menu refer to section 2 2 1 3 to set the experiment timer 2 4 Alarms and Warnings BSC uses alarms to indicate that the condition inside the BSC is not safe for the operator so check the LCD display to understand the cause of these alarms The most common alarm is the SASH ALARM that indicates that the sash is neither at the normal operating height nor at fully closed position UV mode this condition can easily be corrected by putting the sash at the appropriate operation position Another warning that should be acted upon is AIR FAIL which indicates that there is airflow failure The operator should check if there is any obstruction to the airflow and correct it if possible However if the problem continues the operator should stop working as the BSC s protection may have been compromised Call service or Esco s local distributor Other alarms that indicate a failure or an error in the BSC system e AIRFLOW NO will be displayed if the blower is turned off while there is an airflow failure e SASH ERROR POSITION will be displayed if the microprocessor controller detects more than one micro switch activated at the same time which is impossible as the sash can only be at one position at one time This indicates a failure in the sash detection system e
100. tachments should be equipped with an emergency shut off valve that be accessed quickly and with ease should the need arises Check whether there is a need to install pressure regulators to reduce the line pressure e Your BSC can accommodate service fixtures on the left or right hand side of the cabinet Make allowance for the positioning of service lines when planning the installation site to ensure ease of access to emergency shut off valves 4 2 Installation 4 2 1 Connecting the Electrical Supply e First of all refer to the serial label on the BSC for the proper electrical rating e Review the electrical wiring diagrams in Appendix section prior to installation All wiring should be done in accordance with the National Electrical Code e Connect the supplied power cord to the input on the top of the BSC Make sure the cable connector is seated firmly in the socket 4 2 2 Connecting to a PC using RS232 1 Connect the RS232 female connector of the BSC to the RS232 male connector of the PC using DB9 RS232 serial cable 2 Usethese port settings for your RS232 connection o BaudRate 2400 o Data Bits 8 Stop Bits t1 Parity None o Flow Control None If you are using Windows XP s HyperTerminal follow these steps 1 Click Start and select All Programs Accessories Communication HyperTerminal Connect LAZ 2010 41321 Enter datale tor the ghara number that pau ba diat Connectian D
101. tand was used during certification The maximum NSF approved leg levelers adjustment is 50 mm 2 4 1 4 Exhaust Requirements The exhaust filter area is especially susceptible to disruptive air currents or air drafts A clearance of at least 30 cm 17 is recommended between the highest point of the cabinet and the ceiling If the distance is less than 30 cm 1 the airflow alarm system may need re calibration In fact for proper exhaust filter leak scanning purposes a minimum clearance of 50 cm 1 8 is recommended 4 1 5 Electrical Requirements e Ensure that the outlet is rated accordingly to the model requirement The cabinet will not work properly or may even be damaged if it is powered by an incorrect source e Thecabinet s maximum voltage fluctuation is 2 of nominal voltage otherwise install power stabilizer e Surge protection and UPS are strongly recommended for better protection e The power cable is located on the right hand side of the BSC and the cord is 2 5 m long When preparing the installation site try to ensure the outlet is located to the right of the cabinet for ease of access The cabinet should be connected to unobstructed dedicated power outlet s Class Biological Safety Cabinets Ecco 4 1 6 Service Line Requirements e All service lines should be installed by a suitably qualified and certified engineer in accordance with all applicable local state and government regulations e Service line at
102. tion even in the unlikely event of inflow and downflow imbalance that would compromise protection on a conventional cabinet E co Airstream 1 4 2 2 D and 2 G Series Airflow Pattern ULPA Filtered Air Unfiltered Potentially contaminated Air Room Inflow Air e Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum fan plenum at the top of the cabinet e Dual fans dual exhaust filters provide an added measure of protection If the primary fan fails the secondary fan still pushes the air across the exhaust filters to maintain inflow and containment e Approximately 32 of the air in the common plenum is exhausted through the ULPA filter to the room The remaining 6896 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the work surface in clean air e The uniform non turbulent air stream protects against cross contamination within and throughout the work area e Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zo
103. tream Chapter 8 Troubleshooting This guide addresses the most common service issues For more trouble shooting or service information contact your local ESCO Distributor Hardware e DVM Digital Voltage Meter Note An analog meter can be used for troubleshooting but cannot be used for motor voltage measurement e Phillips screwdriver e Insulated jumper cables The Electrical Panel and Component Layout Open the front panel the electrical panel is the red covered box located on the back of the panel The component layout is as followed Electrical Box 660 x 440 mm Bottom a de Hole f r Ter gabe 2 pm for UV swilth 12 muy 2 pin far Ready swilch 1 2 pin tor Fully Open switch 3 pim for flow Sensor 1 nin fer Temperature Sensor E 2 pin far LV intedock switch need Contreras Capabite pe 1 5 i positlan when mh mn 4 Ll k EA 2 cover closed gr rl r gt Capac bay a loh CREB for El dr AGD 8 pin lor lamp ff x i E T 4 pin tor UY lamp E Connector E i far cutest A 5 pin far power supply Connector e N an A 3 5 pin far bows 4 Flugrescen Ballast a 1 citcurl breaker Box Cover AL 3 z a E Fs E E i Molar Motor Speed Controller 2 Speed Ca
104. uge stainless steel type 304 with 4B finish Side Walls 0 9 mm 0 035 20 gauge stainless steel type 304 220 240V AC 320 h 189 cfm 538 h 317 cfm 615 h 362 cfm 823 h 485 cfm 60Hz 1 2 353 2 453 2 553 2 653 Net Weight 180 kg 397 Ibs 218 kg 481 Ibs 256 kg 563 Ibs 305 kg 672 Ibs Sound emission test is conducted open field condition anechoic chamber an Cabinet only excluding retrofit kit amp support stand ECZO Airstream A 9 3 2 D Series 9 3 1 Engineering Drawing DE Y 1270mm 1340mm 50 0 52 7 1870mm 1950mm 73 6 76 7 1 2 12 13 14 5 E E E E 5 E ry 3 7 2 16 Sw e Y 4 E J E O 5 5 aero re AAC a OASIS Y to 1 i A 545mm 21 5 732mm 28 8 B _ g ls 800mm 31 5 1 RS232 Port 7 Fluorescent Lamp s 11 Armrest 2 Power Inlet 8 IV Bar Retrofit Kit 12 2nd Exhaust ULPA 3 Sentinel Gold Provision Filter Controller 9 Plugged Service 13 1st Exhaust ULPA 4 Sash Window Fixture Provision 14 Fan 5 Worktray 10 Electrical Outlet 15 Downflow ULPA Filter 6 Elect
105. ul with the weight distribution Usually it will take 6 or more people to move it For repackaging e Bolt the BSC to the pallet e Strap the BSC body down to the pallet e Repackage as necessary If possible use original packaging e When moving the BSC use material handling equipment and lift the pallet 4 1 2 Environmental Requirements e Indoor use only e Altitude of up to 2000 meter 6600 e Relative humidity between 2096 90 e Temperature between 18 30 C 65 F 86 F e Pollution Degree 2 0 Pollution degree describes the amount of conductive pollutants present in an operating environment In pollution degree 2 0 it is assumed that only non conductive pollutants such as dust are present except that occasional conductivity is cause by condensation 4 1 3 Support Requirements e support cabinetry should be leveled e Thesupport cabinetry must be able to withstand the weight of the cabinet and any apparatus within e support cabinetry must be ergonomics Esco recommends Esco support stand with leveling feet for BSC Without Esco s support stand Esco cannot guarantee the cabinet s resistance against tipping and hence the user would be solely responsible for ensuring that the cabinet is securely fastened to third party support cabinetry The use of non leveling feet Esco support stand will nullify the third party certification NSF or T V that the cabinet may have because only Esco leveling feet support s
106. ult Leaving the UV lamp on for over 60 minutes or even overnight is not recommended because it shortens the lifespan of the lamp The UV lamps used in Esco BSC have a lifespan of 2 000 hours e TheUV lamp should be cleaned of any dust and dirt weekly and changed annually to ensure its effectiveness e The use of UV lamp in BSC has been explicitly discouraged all major international standards and recommendations 3 6 Decontamination and Disinfecting Agents e For stainless steel surfaces all common disinfecting agents except chlorine based ones are suitable e For powder coated surfaces all common disinfecting agents are suitable However the BSC has been specifically evaluated for use with the following o 1Nhydrochloric acid 1N sodium hydroxide 1 quaternary ammonium compound 596 formaldehyde 5 000 ppm hypochlorite 296 iodophor 596 phenol o 7096 ethyl alcohol e Depending on the contaminant involved at the time of operating the BSC there are various other types of disinfecting agents that may be used The following table outlines the effectiveness of various disinfecting agents against the different types of contaminants O O O O O E co Airstream Peroxide Glutaral Paracetic Chlorine Quaiemaly Decontaminant M Chlorine lodophor Alcohol Phenolic Ammonium dehyde acid Dioxide Compounds Acetic acid 565520 Inter Inter Inter Classification Sterilant Sterilant Sterilant High Level Low Level mediate m
107. use is good and the circuit is not energized Terminal J2 A Power into FAN Circuit j 1 RelayLS1 Fuse F1 Normally closed relay connection not used RELAY BOARD Figure 4 1 K1 Relay not Check voltage at the speed control on the motor voltage test points Refer to Figure energized or faulty 4 2 E S series model and Figure 4 3 D G series model If the voltage is correct at the speed control proceed to next step No voltage at the speed control indicates that K1 relay is not energized or the wiring between the Relay board and K1 is open Check the voltage between the BLUE Neutral terminal block and NO terminal contact at K1 relay Refer to Figure 4 4 4 5 Ensure that the motor blower switch in front keypad membrane is on If voltage is present at K1 proceed to check the speed controller in next section Otherwise check all terminal connections to K1 relay If connections on K1 relay are OK visually inspect K1 relay for any burnt or flash marks Refer to Figure 4 5 If burnt or flash marks are present replace the relay Figure 4 2 Figure 4 3 E co Airstream ed A 0 Hormally Open 3 7 y e X 22 lt gt E ji a NET ES E a nm 2 Figure 4 5 Defective motor Switch on the blower on the cabinet speed controller Open electrical box and see Component Layout at begin

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