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1. 9 Loosen the screws fastening the measurement baths The screws cannot be removed from the measurement baths WBC measurement bath 10 Remove the measurement baths and sub baths by pulling them together HGB cover HGB cover screws Measurement bath screws 11 Place a cloth or tissue paper under your hand and remove the aperture cap by pulling it toward you If it is not easy to pull the aperture cap SS ES E move it slowly left and right to remove it Y 12 Carefully rinse the aperture cap Remove all dirt especially from the Ps inside Syringe 13 Insert a syringe containing CLEANACs3 detergent into the aperture cap Concave Aspirate and dispense the detergent by pressing the syringe s plunger up and down to remove the dust around the aperture cap M ion Be careful not to press the syringe with too much force Otherwise the aperture cap may be damaged Aperture The condition of the aperture cap can be checked with a 100x microscope Service Manual MEK 7222 6 17 6 MAINTENANCE Red mark AE Red O ring 6 18 SE o White mark p oo ic w Black O ring White O ring v 14 If clogged dust still remains in the aperture
2. 7 Remove the pump covers by removing the two screws on each cover Pump covers Pump tube Pump tube 6 14 Service Manual MEK 7222 6 MAINTENANCE 8 Pull out the white tube joint from the tube holder and pull out the tube by turning the pump rotator counterclockwise Then pull out the black tube joint from the tube holder Tube holder 9 Remove the white and black tube joints and replace the pump tube with a new one 10 Put the white tube joint back to the original position and push the pump tube into the tube guide by turning the rotator counterclockwise 11 Put the black tube joint back to the original position NOTE e Do not damage the pump tube with the tube guide Do not attach the black tube joint to the tube holder before the white tube joint This causes disconnection of the tube due to compressed internal air e Put back the pump tube properly If the pump tube has slack it will be damaged by the tube guide Make sure the joints are held properly by the tube holder as shown below Otherwise the life of the pump tube will be shortened 12 Reattach the front cover and fasten it with the two screws on each side 13 Turn the power on The h
3. ssssssseeeeneene 8 32 MEK 7222J K Hematology Analyzer Installation Check Sheet 8 34 Service Manual MEK 7222 8C 1 Introduction Demonstration Outline Service Manual MEK 7222 8 DEMONSTRATION GUIDE This demonstration guide provides an installation and setup flow chart for a demonstration of the MEK 7222J K hematology analyzer The installation takes approximately 2 hours Copy the check sheet provided at the end of this manual and use it for checks It is also useful in troubleshooting Do the following steps to install and set up the hematology analyzer for demonstration 1 Prepare the hematology analyzer a Connect the tubes b Connect the power cord and ground the hematology analyzer c Turn the laser switch on d Turn on the power 2 Set the settings and clean the fluid path in the hematology analyzer a Check and set the date and time settings b Clean the fluid path in the hematology analyzer by pressing the clean key on the front panel 3 Adjust the gain and measure background noise a Count the polymer microsphere suspensions to check the CV and PEAK values b Adjust the flow cell unit position c Adjust the gain FS and FL for WBC 5 part differential parameters in rough mode using polymer microsphere suspensions d Measure background noise e Adjust gain for WBC 5 part differential parameters in fine mode using venous blood samples from different healthy people which are 30 min
4. A confirmation messages appears on the screen DRAIN ALL 1 Disconnect diluent detergent and hemolysing reagent tubes from inlet 2 Do not disconnect tube from waste outlet Ces _10_ 4 Press YES to start draining If you press NO the process is canceled and the screen returns to the READY screen 4 5 4 DISASSEMBLY AND ASSEMBLY 4 6 During draining the screen shows the DRAINING ALL message DRAINING ALL 14 FEB O3 19 55 DRAINING ALL PLEASE WAIT When draining is completed the PRESS CLEAN KEY message appears 14 FEB O3 09 24 PRESS CLEAN KEY VERS ION 01 07 5 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For you safety before disassembling the hematology analyzer wait approx 10 minutes after turning off the main power switch Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Opening the Front Cover 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your safety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Remove the 4 screws which secure the front cover to the hematology analyzer chassis 3 Slightly pull the front cover toward you and open it as shown below To assemble the hematology analyzer rever
5. Turn the power on The hematology analyzer starts priming the fluid path Service Manual MEK 7222 6 MAINTENANCE Checking and Cleaning the Cap Pierce Rinse Unit Sampling Nozzle and Cap Pierce Needle Optional MS 721V Cap Pierce Unit Cap pierce needle cover Cap pierce rinse unit 1 Check for dirt around the opening of the cap pierce rinse unit 2 Check for dirt around the opening of the cap pierce needle cover Service Manual MEK 7222 Check and clean dried blood and dirt on the cap pierce rinse unit sampling nozzles and cap pierce needle every month or every 1000 sample countings 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 Check for dried blood or dirt on 1 2 3 and the bottom of the cap pierce rinse unit as shown below Cap pierce rinse unit UTR 4 3 Check for dirt on the sampling nozzle especially tip of the nozzle Wipe any dirt off 1 2 3
6. 3 Press the OPERATIONS key on the MENU screen to display the OPERATIONS screen Service Manual MEK 7222 5 5 5 Adjustment OPERATIONS 14 FEB B3 15 21 PRIME DRAIN BATHS AUTO PRIME DRAIN ALL STRONG CLEAN _ CLEAN FLOWCELL ADJUST FLOWCELL OK 4 Press the PARTICLE TEST key to display the PARTICLE TEST screen PARTICLE TEST 12 FEB 93 14 05 cU 80 0 8 8 2 8 PEAK 8 a a OR GAIN OUTPU 5 Press the MEASURE key The PRESS COUNT KEY TO START message appears and the hematology analyzer is ready for counting the polymer microsphere suspensions PARTICLE TEST 12 FEB 83 14 05 CANCEL PRESS COUNT KEY TO START 5 6 Service Manual MEK 7222 5 Adjustment 6 Put the sampling nozzle into the bottom of the sample container containing the polymer microsphere suspensions so that the tip of the sampling nozzle comes near the bottom of the sample container and press the Count switch The polymer microsphere suspensions is aspirated and measurement starts The MEASURING PARTICLES message appears on the screen and the scattergrams and histograms appear in real time The measurement lasts approximately 40 seconds h Put the sampling nozzle to this level NOTE Do not let the sampling nozzle touch the bottom of the sample container This may prevent aspiration of the polymer microsphere suspensions The result appears on the screen PARTICLE TEST 12 F
7. Acceptable range 1 645 HGB ON Acceptable range 1 5 V to 4 5 V HGB OFF Acceptable range 0 5 V or less MCV Acceptable range 100 15 PLT Acceptable range 160 5 Sensitivity WBC ___ RBC ___ Threshold WBC ___ RBC ___ PLT __ Background noise check Write each value displayed on the screen after pressing the count switch WBC _____ Acceptable range 0 2 or less x 10 1L RBC ____ Acceptable range 0 05 or less x 10 1L HGB Acceptable range 0 1 or less g dL PLT Acceptable range 10 or less x 10 AL TOC Acceptable range 100 or less Check with polymer microsphere suspensions Write each value on the PARTICLE TEST screen when the polymer microsphere suspensions are aspirated with the sampling nozzle FS CV Acceptable range 7 or less FS PEAK FL CV Acceptable range 7 or less FL PEAK TOC Service Manual MEK 7222 1 y mo 7 aJey mo Check with MEK 5DN Hematology Control Write down the lot number of the MEK 5DN hematology control and all the X R data on the X R NORMAL screen when the hematology control is aspirated with the sampling nozzle Lot No of MEK 5DN WBC X NE X LY X MO X EO X BA X RBC X HGB X HCT X MCV X MCH X MCHC X PLT X GR R LORS eR _ R_ ORO A S R___ SORS R___ A PA E A wt e Current Calibration Coefficients Write down the calibration coeffic
8. components in the instrument Removing replacing and installing major components should be done by qualified service personnel WARNINGS e To avoid the possibility of injury to yourself or damage to the instrument do not install or remove any component or change switch settings while the power is on and wait 10 minutes before installing to or removing any component from the instrument after the power is off e To avoid accidental discharge of static electricity which could damage the instrument components use a wrist ground strap when installing or removing any component of the instrument e When replacing any parts or units in the instrument do not touch the site of the instrument where blood is or may be e Wear rubber gloves to prevent infection by blood CAUTIONS e Before connecting or disconnecting any cables turn off the instrument and unplug the AC power cord from the instrument e Fuses cut off the power when an abnormality occurs in the instrument Eliminate the malfunction before replacing the fuse Use the correct fuse only The fuse rating is shown on the holder e Removal and replacement of any component in the instrument should be done by qualified service personnel e Use only parts recommended by Nihon Kohden to assure maximum performance from your instrument e Anti static bench mat e Wrist ground strap e Phillips screwdriver insulated type e Flat blade screwdriver insulated ty
9. pet aci Segel 6 4 16 8 16 8 17 4 17 0 Service Manual MEK 7222 8 DEMONSTRATION GUIDE 4 Check that there is no considerable difference in the listed data and press the OK key to return to the CALIBRATION screen If one data is extremely different from the others delete the data and measure the calibrator again to obtain the data close to other measured data To delete data a Press the DELETE key The SELECT DATA TO DELETE message appears Select the data to delete CALIBRATION MEASURE TIME 14 FEB 03 11 58 2 Pe xm n Po a uw ep E e N CA E N ae CA AO NO CACIGO GO CO N27 INO EE CA o o Ne Met tr m nm ng C0 s CALO 5 GO CO Is JL CALCO s P D UT SO EFICADORRNNN KOUNWUORE A O UND OO NUWWOON OICIGI J A E AS OO NAEUOQON uMNOU AS h TO NUWWOON OIN C GI E E E A ees etl ENGINE OO OO 00051111 DELETE key TZ TTD 3Io0m 3331203331003 ESAS UT 24 2 UD i P P SA SA IUUD Uu OUI JUTUD PO UD CAL AJ CAT EI _ b Press the time key of the desired data The DELETE DATA message appears c Press the YES key to delete the data The screen automatically returns to the CALIBRATION MEASURE screen after deleting the data If you press NO the process is canceled and the screen returns to the CALIBRATION MEASURE screen 5 Press the OK key to return to the CALIBRATION screen NOTE If you
10. EX D013 J D014 D015 D017 SWO015 SW014 SW013 D019 DO11 BIRYOU LED for capillary mode operation When the instrument is in the capillary mode this LED is lit D012 SAMPLE 0 LED for operation indication When the instrument is in standby mode this LED is lit D013 SAMPLE 1 LED for operation indication When the instrument is in standby mode this LED is lit DO14 SAMPLE 2 LED for operation indication When the instrument is in standby mode this LED is lit DOI5 SAMPLE 3 LED for operation indication When the instrument is in standby mode this LED is lit D017 MAIN POWER LED for status indication of the main power switch When the main power switch is set to on this LED is lit DO018 SUB POWER LED for status indication of the Power key When the Power key is pressed this LED is lit DOI9 LASER KEY LED for status indication of the laser switch When the laser switch is set to on this LED is lit SWOII BIRYOU Capillary mode switch SWOI2 TOSYUTU Diluent dispense switch in capillary mode SW013 POWER Power key SWOIA RESET Reset key SWO15 CLEAN Clean key VOLUME board VROI2 OFF SET Variable resistor for offset adjustment of pressure sensor Service Manual MEK 7222 7 9 7 Test Point Variable Resistor LED and Switch on Board POWER board 7 10 D0110 TP0114 TP0113 TPO112 TP0115 TP0118 SWo022 TP0116 D0111 TPO19 TPO110 Swo21 TPO111 TROIA D0114 TPO15 TPO16 TPO11 TP01
11. Test point for protection to a wrong connector connection TP092 Test point for protection to a wrong connector connection TP093 Test point for protection to a wrong connector connection DOS1 RS LED for operation check of the RBC sub bath in the MC 720V CBC measuring unit When the RBC sub bath works this LED lights D052 WS LED for operation check of the WBC sub bath in the MC 720V CBC measuring unit When the WBC sub bath works this LED lights D061 P2 LED for operation check of sensor 2 in the MP 520V pump unit When the sensor is turned on this LED is lit D062 P1 LED for operation check of sensor 1 in the MP 520V pump unit When the sensor is turned on this LED is lit D087 C1 LED for operation check of valve 1 in the MS 721 V cap pierce unit When the valve is turned on this LED is lit D088 C2 LED for operation check of valve 2 in the MS 721V cap pierce unit When the valve is turned on this LED is lit D091 1 LED for operation check of valve 1 in the MJ 721 V sheath unit When the valve is turned on this LED is lit D092 2 LED for operation check of valve 2 in the MJ 721V sheath unit When the valve is turned on this LED is lit D093 3 LED for operation check of valve 3 in the MJ 721V sheath unit When the valve is turned on this LED is lit D094 4 LED for operation check of valve 4 in the MJ 721V sheath unit When the valve is turned on this LED is lit D095 5 LED for operation check of v
12. UP VR VR042 LOW VR D042 LOWER TP041 UPE TP012 ED TP042 LOWE 1 Open the front cover according to the procedure described in Opening the Front Cover of Section 4 Disassembly and Assembly 2 Press the OPERATIONS key on the MENU screen The OPERATIONS screen appears 3 Press the PRIME key on the OPERATIONS screen The PRIMING message appears on the screen When the WBC and RBC measurement baths fill with diluent watch the manometer window as shown above and check that the manometer also fills with diluent Also check that the two LEDs D041 UPPER and D042 LOWER light Press the OTHER key on the MENU screen The OTHER screen appears Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen which displays each sensor output voltage appears 6 Adjust the VR041 UP VR and VR042 LOW VR variable resistors on the MANOMETER board so that each sensor output voltage is 1 5 V or less 7 Press the count switch and watch the diluent level in the manometer When the diluent level is lower than the bottom of the manometer press the reset key Check that there is no diluent in the manometer Also check that the two LEDs D041 UPPER and D042 LOWER do not light Press the OTHER key on the MENU screen The OTHER screen appears 9 Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen which displays each sensor output voltage appears 10 Adjust the VRO41 UP VR an
13. and the bottom of the cap pierce rinse unit with the cotton swab if there is dried blood or dirt 6 21 6 MAINTENANCE NOTE To remove crystals of blood or salt from the sampling nozzles wipe them off with a gauze or a cotton swab moistened with CLEANAC 3 detergent 7 Reattach the front cover and fasten it with the two screws on each side 8 Turn the power on The hematology analyzer starts priming the fluid path 9 Display the OPERATION HISTORY screen and press the RESET key for NEEDLE to reset the counts to zero OPERATION HISTORY 26 AUG OS 15 20 TOTAL HOURS enn hours ee E o PEIRE 8 1000 counts RESET Wee 8 3000 counts RESET NEEDLE CS1F2 gt RESET key 6 22 Service Manual MEK 7222 6 MAINTENANCE Checking and Replacing the Cap Pierce Needle Optional MS 721V Cap Pierce Unit Check that there is no blood clot dirt or damage on the cap pierce needle every month or every 1000 sample counts If there is any damage to the cap pierce needle replace it with a new one according to the following procedure WARNING The cap pierce needle is sharp and sample blood may have contacted it Be careful not to prick yourself with the cap pierce needle OPERATI ONS 14 FEB 03 15 21 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub ba
14. 00009 I al O No Name Laser switch 1503 Diluent inlet CLN Detergent inlet CLN3 Detergent inlet HEMO3 HEMO3N Hemolysing reagent inlet HEMOS Hemolysing reagent inlet Description Turns the laser on or off with the laser key for WBC 5 part differential measurement Inlet for the ISOTONAC 3 diluent Inlet for the CLEANAC detergent Inlet for the CLEANACe3 detergent Inlet for the HEMOLYNACe3 or HEMOLYNACe3N hemolysing reagent Inlet for the HEMOLYNAC S5 hemolysing reagent WASTE Waste outlet Outlet for waste such as used lyse detergent and aspirated samples Service Manual MEK 7222 When using the HEMOLYNAC 3N reagent attach the HEMO3N label provided with the hematology analyzer to cover the HEMOS label at the HEMO3 inlet 1 9 1 GENERAL Rear Panel A Refer to warnings and cautions in Connecting an External Instrument to the t Hematology Analyzer in E 2 Section 2 of the operator s ae manual o 4 o 6 7 Refer to warnings and cautions in Connecting the Power Cord and Grounding the Hematology Analyzer in Section 2 of the operator s manual No Name Description 1 ou Connects to the optional WA 460V card printer other external P printer or PC Serial port 1 Connects to the optional handy bar code reader Ca
15. 1 Noise is detected during counting A 042 RBC LEVEL 2 Then recount the sample If the alarm still occurs perform strong A 043 RBC LEVEL 3 The RBC aperture cap is clogged cleaning clean the aperture cap and replace the pump tube Press the Clean key to perform cleaning Then recount the sample A 044 RBC CLOG The RBC aperture cap is clogged If the alarm still occurs perform strong cleaning clean the aperture cap and replace the pump tube A 045 RBC BUBBLE 1 A 046 RBC BUBBLE 2 Press the Clean key to perform cleaning A 047 RBC BUBBLE 3 A a REC Ol Then recount the le A 048 RBC BUBBLE 4 S Add the diluent or check that the connection APOGA NENET oe tube is connected properly Perform counting A 049 RBC PRIME ERROR or the connection tube is out of ae e EN again after priming or cleaning the position hematology analyzer Prepare the sample and measure again A 050 RBC SAMPLE ERROR Abnormal sample Check that the temperature of the diluent is between 15 and 30 C A 051 RBC NOISE 2 Check the grounding and separate the instrument from other equipment and their power sources Then recount the sample A 060 UPPER MANO ERROR The upper part of the manometer is dirty Manometer sensor output is low A 061 LOWER MANO ERROR The lower part of the manometer is dirty Manometer sensor output is low Press the Clean key to perform the cleaning Then press STRONG CLEAN on the O
16. 10 from the board 6 To remove the LCD unit put the front panel unit face down on a table covered with a clean soft and smooth antistatic material disconnect the 3 cables cables 2 to 4 from the KEY DISPLAY board remove the 6 screws screws 11 to 16 from the LCD unit chassis and remove the LCD unit chassis from the front panel unit by pulling the LCD unit chassis toward you NOTE If the front panel unit is not put face down on a flat space the following key top covers and spacers will be apart from the original positions Key top covers Spacers Key top covers Service Manual MEK 7222 4 35 4 DISASSEMBLY AND ASSEMBLY 7 Turn the LCD unit chassis face up on the table 8 Remove the 4 screws which secure the LCD unit to the chassis Remove the LCD unit from the chassis Replacing the MS 721V Cap Pierce Unit Option 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Move the sampler unit leftward by hand 5 Disconnect the 2 tubes from the rinse unit 6 Disconnect the 2 cables connected to the cap pierce unit at the DRIVER board 7 Remove the 4 screws which secure the cap pierce unit to the chassis 8 Pull the cap pierce unit toward
17. 160 0 412 8 120 120 0 716 0 ra v0 0 OVER 1v Press the YES key to delete selected data 4 Press the OK key on the PEAK LIST screen to return to the AUTO DIFF GAIN screen 5 14 Service Manual MEK 7222 5 Adjustment Manually Adjusting the Gain The gain can be manually adjusted lt Adjusting the gain with automatic calculation function gt 1 Measure 10 human blood samples from different healthy persons which is 30 minutes to 8 hours after collection for all 22 parameters 2 Press the DIFF key on the SENS amp THRESHOLD screen MAIN 5459 ED 2 ED 611 28 s aor 17008 LOK REDRAW CALC 3 Select BLOOD for the sample type to display the scattergram FS FL and SD of the blood sample counted in step 1 4 Repeat steps 3 and 4 of the procedure in the Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions section to automatically adjust gain for FS FL and SD lt Gain adjustment with numeric keypad gt 1 Select the gain to be changed FS FL SD or THR 2 Enter the value with the number keys The entered value is displayed in the VALUE box 3 Press the ENTER key to enter the value for the selected gain Compensating Dilution Ratio Service Manual MEK 7222 When the cylinder block is disassembled and assembled or replaced with a new one perform the compensation of the dilution ratio Refer to the following example The label of the def
18. 3 8 Disassembly and Assembly eeeeeeeesus 4C 1 Betore You BOQgl uu eue cuite euet tert T aii 4 1 Warnings and Cautions RR 4 1 Required TOONS c ies 4 1 Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the sip EET 4 2 Board anid Unit LOCAtIONM P 4 3 Right Side ica a E a A EN aE a EEEE TE A 4 3 Ee ft 4 3 Upper Part of Right Side ini etr dene cete ttr in ee rog ERR 4 4 Lower Part Of RONE SIAE siiin rt tente ere baee dere gra terry S a e ERE dacs 4 4 Iioc 4 4 Draining the Hematology Analyzer ssssseeeeeeeeennnm eene nennen 4 5 Opening the Front Cover macia ii 4 7 REMOVING the Top COVEN visir dis 4 8 Replacing the Boot ROM ssssssssssseseeee eene cnn rre nan n rra ren nr rca rra ran rn ca nennen enne 4 9 Removing the Rear Cover Including the Power Supply Unit ssesees 4 11 Removing the CBC Measuring Unit oooococonnnccccnnccccconcccnnonancnnnnncconnnnn ca nnnn nc nn nnnn cnn rann cnn 4 12 Removing trie Diluter Unit a 4 13 Removing the Sampler Unit ina ias 4 15 Removing the Pump Unit enn nenne rennen 4 16 Removing the Triple Pump Unit cusco aia 4 18 Removing the WA 720VK Printer Unit Option oooonoccccnnnccocinaccccnanaccnonanncnanancanonnnconannncanno 4 19 Removing the Front P
19. 64 61 v DDD DIS ED 21 D 61 OO 30 rap 7 099 FS FL CALCULATE key MEASURE key ENTER key Measuring Background Noise 8 16 CA Press the sample type key to select PARTICLE to display the scattergram and the peak for the polymer microsphere suspensions Press the CALC key The appropriate setting value of FS calculated from the NOW peak and AIM peak appears in the VALUE box Press the ENTER key The value displayed in VALUE is entered in FS and the cursor moves to FL Press the CALC key The appropriate setting value of FL appears in the VALUE box Press the ENTER key to register the value for FL NOTE In PARTICLE mode SD gain cannot be adjusted SD gain is adjusted in fine mode by using human blood samples Press the MEAS key to return to the PARTICLE TEST screen to count the polymer microsphere suspensions to check the CV and peak value with the adjusted gain Repeat the procedure in the Counting Polymer Microsphere Suspensions to this section Adjusting the Gain FS and FL for WBC 5 Part Differential Parameters in Rough Mode Using Polymer Microsphere Suspensions until you obtain the optimum peak value Display the READY screen and press the Count switch to count the diluent There is no need to set the sample The result appears after measurement is complete VENOUS 14 FEB 03 09 35 105 uL BLOOD ID 88003 MIS NRA Wot 105 x
20. Baths and Sample Cup Check the sub baths measurement baths and sample cup every day Clean the measurement baths sub baths and sample cup when there is any blood stain or dust on them Once a month or every 1000 sample counts NOTE Be careful not to damage the sub baths and measurement baths The baths are made of special plastic 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths o O y 3 Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 10 Service Manual MEK 7222 6 MAINTENANCE 6 Check the WBC and RBC measurement baths sub baths and sample cup If there is any blood stain or dust on them remove and clean them taking the following steps Sample cup 7 WBC measurement bath HGB cover Measurement bath screws 8 9 Remove the joint of the sample cup Hold the ta
21. Increased WBC An extreme increase in WBC causes excessive light scatter In these cases measure manually Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer 2 Increased lipids The blood sample may be milky when there is excessive lipids This may occur with hyperlipidemia hyperproteinemia and hyperbilirubinemia Accurate HGB measurement can be achieved by manual methods and a plasma blank 3 Increased turbidity When RBC are resistant to lysing turbidity may increase causing increase in HGB Observe if MCH and MCHC values are abnormal HGB result affects MCH and MCHC result 4 Fetal bloods The mixing of fetal and maternal blood may increase HGB value 5 High WBC levels Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the blood sample is high MCH and MCHC levels also become high Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually Service Manual MEK 7222 8 29 8 DEMONSTRATION GUIDE MCV MCH MCHC PLT MPV 8 30 Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually Excessive number of large PLT Excessive number of large PLT and or excessively high WBC may affect the MCV value Check by careful examination of the stained blood film MCH is d
22. LED is lit D0917 17 LED for operation check of valve 17 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0918 E1 LED for operation check of spare valve 1 When the valve is turned on this LED is lit D0919 J3 LED for operation check of valve 3 in the MJ 720V inlet outlet unit When the valve is turned on this LED is lit D0920 J4 LED for operation check of valve 4 in the MJ 720V inlet outlet unit When the valve is turned on this LED is lit D0921 E2 LED for operation check of spare valve 2 When the valve is turned on this LED is lit 7 16 Service Manual MEK 7222 Section 8 Demonstration Guide INTOdUC HO esperanto ra optado 8 1 Demonstration Outline scitis innii cnn rancia naar a Eaa ai 8 1 Required Items for Demonstration oooonoocccccncnnncocccnnccnnnnonncnnncnnnnnnncnn nro nan en 8 2 Preparing the Hematology Analyzer sssssssssseeeneeeeee eene nennen 8 4 Installation Flowechartacosin coe do exeunt tree dano ride 8 4 Connecting TUDES cas oai annees tta cera eek Eae n pes radio eu Er ese Eu eaae pa vu 8 4 Connecting the Power Cord and the Grounding the Hematology Analyzer 8 6 Turning the Laser Switch ON coe tester the etre i ceca tak Yes naani 8 6 Turning On the POWER 1 erret tried 8 7 Setting the Date amp Time and Cleaning the Hematology Analyzer s 8 8 Checking the Date and Time Settings sssusa aiea 8 8 Cleaning the Fluid Path in the Hemato
23. MO 2 to 10 within 20 0 CV WBC 4 0 to 9 0 x 107 uL EO 2 to 10 within CV30 0 gt 2 or average value 1 0 to 2 WBC 4 0 to 9 0 x 10 uL BA 0 to 3 LY Lymphocyte count 0 to 99 9 x 10 uL MO Monocyte count EO Eosinophil count 0 to 99 9 x 10 UL 0 to 99 9 x 10 uL BA Basophil count RBC Red blood cell count 0 to 99 9 x 10 uL 0 to 14 9 x 10 uL within 1 596CV 5 0 x 10S uL HGB Hemoglobin concentration 0 to 29 9 g dL within 1 5 CV 16 g dL HCT Hematocrit 0 to 99 9 MCV Mean cell volume 20 to 199 fL within 1 096CV 70 to 120 fL MCH Mean cell hemoglobin 10 to 50 pg MCHC Mean cell hemoglobin concentration 10 to 50 g dL RDW Red blood cell distribution width 0 to 50 PLT Platelet count 0 to 1490 x 10 uL within 4 0 CV 3 0 x 10uL PCT Platelet crit 0 to 2 9 MPV Mean platelet volume 0 to 20 0 fL PDW Platelet distribution width 0 to 50 Detection Method Blood cell count Hemoglobin Hematocrit WBC population Platelet crit RBC distribution width Platelet distribution width Standardization Analysis Method WBC ICSH1988 Light scatter by laser Electrical resistance detection Cyanmethemoglobin optical detection Histogram calculation Histogram calculation Histogram calculation Histogram calculation Clin Lab Haematol 10 203 212 1988 RBC ICSH1988 Clin Lab Haematol 10 203 212 1988 Service Manual MEK 7222 ICSH T
24. Microsphere Suspensions sssssee 6 4 Measuring the MEK 5DN Hematorogy Control ssssssseeeneenee 6 4 Current Calibration Coefficients ooooononnccnnnccccnnncccnnnoccnconacnnnnnrrncnanoncn canaria nnne 6 4 New Calibration Coeffisients sssssssssssesseseeeeeneen nennen nennen nennen 6 4 eye Irene EET 6 5 Checking the Operations oooooocccinnccccononccnnnnocanonancnnnnnononnnnn cnn naar nn nan n conan nennen nnne 6 5 Built in Printer Unit Operation When the printer is installed 6 5 External Printer Unit Operation When the external printer is connected 6 6 Bar Code Reader Operation WHen the bar code reader is installed 6 6 Gul 6 6 Displaying Operation History Screen sssssssssssssesseeeeeeeeeenenenn nennen nnns 6 7 erre n 6 7 Displaying the OPERATION HISTORY Screen sssssssssseeneeeenee 6 7 Checking Cleaning or Replacing Filters sessseeeeeeeenmnneennn 6 8 Checking and Cleaning the Sub Baths Measurement Baths and Sample Cup 6 10 Checking and Cleaning the Rinse Unit and Sampling Nozzles s 6 12 Checking and Replacing the Pump Tubes ssssssseeeeeeeeeeeneeee nennen 6 14 Cleaning the Aperture Caps moria ai 6 16 Checking and Re
25. Printer Unit WA 720VK The printer unit is installed into the front unit The pinter unit prints the numeric data scattergrams and histograms on thermal roll paper Rinse Unit and Switch Assembly The rinse unit cleans the sampling nozzle that blood clots can build up on the surface The count switch is included in this assembly 3 6 Service Manual MEK 7222 3 BOARD UNIT DESCRIPTION Laser Optical Unit Laser optical unit Flow cell adjuster The laser optical unit consists of optical components such as the laser diode and flow cell To differentiate WBC into 5 parts a light scatter technique flow cytometry with laser is used The laser optical unit detects the light scattered by the hemolyzed cells which pass in single file The scattered light is converted to electrical signals Flow Cell Adjuster The flow cell adjuster adjusts the position of the flow cell in the laser optical unit with a stepping motor AMP CONTROL Board The AMP CONTROL board controls operations such as measurement and cleaning the fluid path This board amplifies the analog signals which are output from the CBC measuring unit converts the analog signals to digital signals receives the signals from the switches and sensors and sends the control data to the DRIVER board so that the valves and motors are optimally controlled Service Manual MEK 7222 3 7 3 BOARD UNIT DESCRIPTION DRIVER Board The DRIVER board receive
26. RBC aperture caps 556827 2 pcs Periodic Maintenance Check Procedure Preparation Appearance Safety Reagents Service Manual MEK 7222 According to Section 2 Preparation of the operator s manual turn on the power and check the obtained data from the hematology analyzer before the periodic maintenance check by counting the diluent and MEK 5D hematology control The obtained data must be printed out with the optional printer or written for the record of the maintenance check Also check that the Priming operation is normal after turning on the power Prepare a container syringe and CLEANAC 3 detergent to clean fluid path components such as aperture cap When calibration in capillary mode is required prepare the T857 sample cup and T812 or T813 micro cap Check that there is no damage or fluid leakage on the hematology analyzer Checking the power cord Check that there is no damaged AC plug and exposed wire on the power cord Check that the 3 pin plug type power cord is used and the 3 pins and plug housing are not deformed Check that the resistance of the protective ground line of the power cord is 0 1 U or less by using an earth tester or check the continuity with a multimeter Check that the earth leakage current is 0 5 mA or less under normal condition Check that the earth leakage current is 1 0 mA or less under each single fault condition Check that Nihon Kohden recommended diluent det
27. Replacing Filters oooooocnnnnnncnnncccnnncccccnncccncnnnrnanannrcc non ca rca canaria 6 8 Checking and Cleaning the Sub Baths Measurement Baths and Sample Cup 6 10 Checking and Cleaning the Rinse Unit and Sampling Nozzles 6 12 Checking and Replacing the Pump Tubes ssssssssesseeeseeeeeneeeen nennen nnns 6 14 Cleaning the Aperture Caps oococcccccccccononcccononcnnnnnncononnn conan ocn rnnn rra cnn rra nn carac aran rra 6 16 Checking and Replacing the Sampling Nozzles ooconocccinncccnnnococcnnnccnnonnanc nan ncn nana nana 6 19 Checking and Cleaning the Cap Pierce Rinse Unit Sampling Nozzle and Cap Pierce Needle Optional MS 721V Cap Pierce Unit ssssene 6 21 Checking and Replacing the Cap Pierce Needle Optional MS 721V Cap Pierce Unit 6 23 Calibrating Touch Screen ooccccnnccccnncccccononncnanoncnnonn nn nono nora nn ran rra rra 6 25 Maintenance Check Sheet rrinin aaae cnn nc nn nana aaea A hE Eas 6 26 Service Manual MEK 7222 6C 1 6 MAINTENANCE To be Replaced Periodically Description NK code Qty per instrument O ring for manual mode sampling nozzle 315366 1 pe Filter for measurement bath and air trap 6144 0018990 2 pe Filter packing for measurement bath and air trap 2114 082062B 2 pes Pump tube 2114 080599A 2 tubes O ring white for WBC aperture cap 553198 1 pe O ring red for RBC aperture cap 553233 1 pc O ring black for WBC and
28. START 6 5 adjusting flow cell position PEAK 44 76 5000 5000 CLEAR J MEASURING PARTICLE 5 8 Service Manual MEK 7222 5 Adjustment 12 After adjusting the flow cell to obtain the optimum result count the polymer microsphere suspensions again to make sure the optimum results can be obtained again Refer to step 6 If the CV of ES and FL are below 7 and the peaks match the following value go to Adjusting Gain for WBC 5 Part Differential Measurement Finely by Human Blood section If the CV of ES and FL are below 7 but the peaks do not match the following value go to Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions section FS Peak 61 3 FL Peak 90 3 Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions FS Forward Small Size vertical 1 Display the SENS amp THRESHOLD screen for WBC 5 part differential axis of the parameters by doing one of the following scattergram a Press the GAIN key on the PARTICLE TEST screen FL Forward Large Complexity b Press the SENS amp THRESHOLD key on the SETTINGS screen and press the horizontal axis DIFF key NOW Current peak SD Side Granularity AIM Target peak hori tal axi horizontal axis Paes nD VERE THR Threshold of FS PARTICLE2 Press this key to select sample type from PARTICLE BLOOD and CONTROL In BLOOD mode the scattergram changes among MAI
29. according to Section 5 Adjustment MANO UPPER MANO LOWER DILUENT HEMOLYNAC 5 and HEMOLYNAC 3 1 5 V or less ELECTRODE 17 7 V to 18 3 V HGB LED ON 1 5 V to 4 5 V Adjust this voltage for 2 9 V to 3 1 V if it is out of 1 5 V to 4 5 V HGB LED OFF 0 5 V or less Press the MENU key on the READY screen The MENU screen appears Press the OTHER key on the MENU screen The OTHER screen appears Press the CIRCUIT CHECK key on the OTHER screen The CIRCUIT CHECK screen which displays the check result of specified measurement parameters appears Check that the following values are displayed on the CIRCUIT CHECK screen WBC 8 045 RBC 1 645 HGB ON 1 5 V to 4 5 V HGB OFF 0 5 V or less MCV 100 15 PLT 160 5 6 3 6 MAINTENANCE Background noise Measuring the Polymer Microsphere Suspensions Measuring the MEK 5DN Hematology Control Current Calibration Coefficients New Calibration Coefficients 6 4 Sensitivity and threshold WBC 5 sensitivity 4 threshold RBC 5 sensitivity AT AUTO or 5 threshold PLT 5 threshold 1 Press the count switch to count the diluent so that the background noise is checked Refer to Measuring Background Noise in Section 2 Preparation of the operator s manual The measurement result appears 2 Check that the following values are displayed on the screen WBC 0 2 or less x 103 iL RBC 0 05 or less x 109AL HGB 0 1 or less g dL PLT 10 or
30. attached to the hematology control b Count more than 10 venous blood samples from different healthy people which are 30 minutes to 8 hours after collection and make sure that there is no flag such as Left Shift Blasts Immature Granulocyte or Atypical Lymphocytes displayed Checking Data with MEK 1 Press the ORDER key on the READY screen or the measured data display 5D Hematology Control screen to display the ORDER screen TCS 12 FEB OS 16 32 D 2009 6 ui j 8 F H5 ID 0001 3 PARAMETERS 2 2 8 SAMPLING CLOSED SAMPLE TYPE BLOOD VERS 1ON 02 01 eu o Hoss reus 2 Select CONTROL as the sample and select 22 as the parameters ORDER 88 SEP G3 89 27 ID 0001 o 22 D GED el SAMPLE m Press OK key to return to ED a READY screen 3 Press the OK key to return to the READY screen 4 Measure the MEK 5D hematology control The result appears on the screen 5 Check that the obtained data falls within the acceptable range on the assay sheet attached to the hematology control Service Manual MEK 7222 8 27 8 DEMONSTRATION GUIDE Checking Data with Human Count more than 10 venous blood samples from different healthy people which are Blood Samples 30 minutes to 8 hours after collection and make sure that there is no flag such as Left Shift Blasts Immature Granulocyte Atypical Lymphocytes or Eosinophilia displayed WARNING Always w
31. bath for 30 minutes and measure the sample immediately RBC Leukemia An increase in WBC in leukemia patient causes increase in RBC Agglutinated RBC Agglutinated RBC may decrease RBC count This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Cold agglutinins IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and increase MCV HGB Turbidity of the blood sample Any physiologic and or therapeutic factors may increase HGB In such a case determine the cause of turbidity and follow the appropriate method below 1 Increased WBC An extreme increase in WBC will cause excessive light scatter In these cases measure manually Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer 2 Increased lipids The blood sample may be milky when there is excessive lipid This may occur with hyperlipidemia hyperproteinemia and hyperbilirubinemia Accurate HGB measurement can be achieved by manual methods and a plasma blank 3 Increased turbidity When RBC are resistant to lysing turbidity may increase causing increase in HGB Observe if MCH and MCHC values are abnormal HGB result affects MCH and MCHC result 4 Fetal bloods The mixing of fetal and maternal blood may increase HGB value HCT Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manuall
32. dae Replace the diluent detergent or hemolysing A 003 NO HEMOLYNACe3 hemolysing feapent reagent Then press the PRIME key on the A 005 NO CLEANAC i OPERATION screen A 007 NO HEMOLYNACe5 A 021 WBC LEVEL 1 Broncos Operation duine counting Press the Clean key to perform cleaning A 022 WBC LEVEL 2 Then recount the sample If the alarm still occurs perform strong A 023 WBC LEVEL 3 The WBC aperture cap is clogged cleaning clean the aperture cap and replace the pump tube Press the Clean key to perform cleaning Then recount the sample A 024 WBC CLOG The WBC aperture cap is clogged If the alarm still occurs perform strong cleaning clean the aperture cap and replace the pump tube A 025 WBC BUBBLE 1 A 026 WBC BUBBLE 2 Press the Clean key to perform cleaning A 027 WBC BUBBLE 3 e A ones Then recount the E i A 028 WBC BUBBLE 4 a Add the diluent or check that the connection ISSULHCIeH e ope tube is connected properly Perform counting A 029 WBC PRIME ERROR or the connection tube is out of zi s cy again after priming or cleaning the position hematology analyzer AAWE NOMET Noise is detected during counting T ne e ups A 031 WBC NOISE 2 power sources Then recount the sample Prepare the sample and measure again A 032 WBCSAMPLE ERROR Abnormal sample Check that the temperature of the diluent is between 15 and 30 C A 041 RBC LEVEL 1 Press the Clean key to perform cleaning 052 PLT NOISE
33. eTO i EA 0 SS r I O O NOTE e Replace the filter periodically e When attaching the filter joint assembly be careful not to bend or damage the filter packing at the bottom of the measurement bath e When there is a leakage check that there is no scratch or damage to the circumference of the filter Service Manual MEK 7222 1 11 1 GENERAL Composition Standard Composition MEK 7222K MEK 7222J CD 720V MA 720V MC 720V XP 512V MD 630V UT 7119 XP 513V MJ 720V UT 7179 UT 7133 UT 71341 UT 7137 Chassis Flow Cell Adjuster CBC Measuring Unit CBC Measuring Board Set PRE AMP Board MANOMETER Board HGB AMP Board HGB LED Board MV CONNECTION Board LED Board 2 way Valve Diluter Unit DILUTER DRIVER Board 3 way Valve Inlet Outlet Unit 4WAY LIQUID SENSOR Board 3 way Valve Sheath Unit SHEATH Board 2 way Valve 3 way Valve 3 way Valve Service Manual MEK 7222 MO 820V MF 820V UT 7172 UT 7173 UT 7174 UT 7175 MS 720V UT 7165 UT 7166 XP 513V PV 720V UT 7188 UT 7162 UT 7163 UT 7164 Service Manual MEK 7222 UT 7167 UT 7168 SC 720VJ for MEK 7222J SC 720VK for MEK 7222K 1 G
34. mode press this Value is calculated from the peak key to return to the PARTICLE NOW and AIM and the calculated TEST screen gain is displayed in the VALUE box The 3 types of histogram Particle Blood and Control and its PEAK value can be displayed with the sample type key on the SENS amp THRESHOLD screen for Diff NOW PEAK is the current peak of the displaying scattergram and AIM is the target peak If the gain is adjusted to the most appropriate value the values of NOW and AIM match If the difference between the NOW value and AIM value is more than 5 the gain must be adjusted NOTE Check that the CV of FS and FL are optimum less than 7 0 by counting the polymer microsphere suspensions before adjusting gain If the CV is not optimum count the polymer microsphere suspensions again after cleaning the fluid path in the hematology analyzer by Adjusting the Flow Cell Unit pressing the Clean key on the front panel or CLEAN FLOWCELL key Position and Counting the Polymer on the OPERATIONS screen and check that the CV values are Microsphere Suspensions sections optimum Adjust the gain only when the CV is optimum If the CV of the polymer microsphere suspensions is still not optimum after cleaning and adjusting the flow cell position contact your Nihon Kohden distributor Service Manual MEK 7222 8 15 8 DEMONSTRATION GUIDE Sample type key SENS amp THRESHOLD 12 FEB O3 15 41 x gt NOWA IM FS VALUEL
35. nennen nnn 8 1 Required Items for Demonstration oooonnnnccccnonnnnonccnnnnnnnnnccnnnnnnnn narran 8 2 Preparing the Hematology Analyzer 0 ccccceeesseeeeeeeeeesneeeeeeeeeennaeeeeeeeesneaeeeeeeeennseeseeeeees 8 4 Installation Flowchart cuac 8 4 Connecting TUDES mexicana 8 4 Connecting the Power Cord and the Grounding the Hematology Analyzer 8 6 Turning the Laser Switch On oooccccinccccnnonocanonannnnonnn cnn nono co nonnn cnn nano nnn narran Raana nan 8 6 Turning OMmthe Power me T 8 7 Setting the Date amp Time and Cleaning the Hematology Analyzer ssseseeeses 8 8 Checking the Date and Time Settings ocooocccinnnccconncccnnonocacinnnnccnnnnnanonnn ca nann no nann cnn nano 8 8 Cleaning the Fluid Path in the Hematology Analyzer ssse 8 8 Adjusting Gain and Measuring Background Noise sss 8 9 ier m o O pecaueesusttnecdedsaset E EET 8 9 Reference Principle of Differentiating WBC ssssseseenenm 8 9 Counting the Polymer Microsphere Suspensions eeseeeeeee 8 10 Adjusting the Flow Cell Unit Position ssesssseeeeeeeneneennenn nns 8 12 Adjusting Gain FS and FL for WBC 5 Part Differential Parameters in Rough Mode Using Polymer Microsphere Suspensions sessssseeeeeeennnee 8 15 Measuring Background NOISE oastei eene 8 16 Adjusting Gain for WBC 5 Part Differential Parameters in Fine M
36. on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 Remove blood or salt crystals on the rinse unit cap and the tip of the sampling nozzles with a cotton swab or cloth moistened with CLEANAC 3 detergent Rinse unit cap Sampling nozzles Tip of sample nozzles 6 12 Service Manual MEK 7222 6 MAINTENANCE Sampling nozzle plate 7 Slide the sampling nozzle plate to the left 1 1 Ia T4 o 1 8 Turn the rinse unit cap counterclockwise to remove the rinse J T f unit 9 Loosen the joint assemblies to remove the tubes Be careful not to lose the O ring from the rinse unit 10 Insert a cotton swab into the rinse unit from the bottom and push out the O ring to remove it from the rinse unit 11 Wipe the inside of the rinse unit and rinse unit cap with a cotton swab moistened with CLEANAC 3 detergent If they are still dirty soak them in CLEANAC 3 for about 10 minutes w 12 Rinse the rinse unit rinse unit cap and O ring w
37. performance Therefore during the operation of the equipment and or system if there is any undesired deviation from its intended operational performance you must avoid identify and resolve the adverse electromagnetic effect before continuing to use the equipment and or system The following describes some common interference sources and remedial actions 1 Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or cellular phone Install the equipment and or system at another location if it is interfered with by an emitter source such as an authorized radio station Keep the emitter source such as cellular phone away from the equipment and or system 2 Radio frequency interference from other equipment through the AC power supply of the equipment and or system Identify the cause of this interference and if possible remove this interference source If this is not possible use a different power supply 3 Effect of direct or indirect electrostatic discharge Make sure all users and patients in contact with the equipment and or system are free from direct or indirect electrostatic energy before using it A humid room can help lessen this problem 4 Electromagnetic interference with any radio wave receiver such as radio or television If the equipment and or system interferes with any radio wave receiver locate the equipment and or system as far as possible from the radio wave receiver If the
38. power line Set the correct date and time setting on the DATE amp TIME screen Refer to Section 3 The time displayed on the J upper right corner of the Scattergrams appear outside The backup battery is old Bubbles in the flow cell unit Incorrect flow cell position Incorrect gain for WBC 5 part differential parameters Check the date and time setting on the DATE 8 TIME screen and turn off and on the power of the hematology analyzer If the time is incorrect replace the backup battery with a new one Contact your Nihon Kohden distributor Clean the flow cell unit Refer to Section 9 2 7 Section 3 Board Unit Description CBE Measuring Un m M M 3 1 BI m cc 3 2 Pump Onil PL 3 2 NIU omar 3 3 sr as aBpre EE 3 3 Power Supply Unit and POWER Board sse eaa enne nnne 3 4 Inlet Qutlet Unit v EE 3 4 Sheath UNM se 3 5 Front Panel Units ete a 3 5 Optional Printer Unit WA 720VK ssessssssssesessseseeenneeee nennen nnn entren sentite sent senes 3 6 Rinse Unit and Switch Assemibly siria tinet adata eiii 3 6 Laser Optical Unit EE 3 7 gen 3 7 AMP CONTROL Board E 3 7 DRIVER Board sisecs cess ss 3 8 Optional Cap Pierce Unit MS 721V cccinncccnnncccccnnannnnann cnn nana ncn cnn a aa aa aE A EAER 3 8 Service M
39. return to the MENU screen or go to another screen except for the CALIBRATION screen from the CALIBRATION MEASURE screen all measured data are automatically deleted After calibration all measured data are automatically deleted You can perform calibration by entering either the calibration coefficient or the new measured value Calibration by Calibration Coefficient 1 Press the VENOUS key on the CALIBRATION screen to display the CALIBRATION VENOUS screen NOTE Write down the current settings before changing them Service Manual MEK 7222 8 23 8 DEMONSTRATION GUIDE New calibration coefficient Previous calibration coefficient x 8 24 14 FEB a3 12 00 CALIBRATION VENOUS CAL GQ 225 JE Eo Mk UN EMER MCHC 2 Calculate the new calibration coefficient based on the following formula The calibration coefficient is on the assay sheet provided with the hematology control and the mean measure value which were obtained in the Measuring the MEK 3DN Hematology Control procedure appears in the DATA column Calibrator assay value Mean measured value 3 Select the desired parameter by pressing the parameter key As you press the key the cursor moves between the DATA and CAL fields Measured value Calibration coefficient CALIBRATION VENOUS 14 FEB S 12 00 Parameter key DAT 7 Setting value 2 4 Move the cursor to the CAL field and enter the new calibration
40. s dL TOC Check the value Service Manual MEK 7222 Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous Blood Samples Service Manual MEK 7222 8 DEMONSTRATION GUIDE 2 Check the measured values on the result screen Make sure that the values are less than or equal to the following values WBC 0 2 x 10 UL RBC 0 05 x 10 uL HGB 0 1 g dL PLT 10 x 10 uL TOC 100 count TOC total optical count This parameter only appears in a background noise measurement Disregard the other parameter values because noise does not affect the other parameters If the values are greater than the above values check the following items press the Clean key to clean the fluid path and recount the diluent The diluent is clean No bubbles in the diluent The apertures and aperture tubes are clean The aperture is firmly attached The measurement baths and sample cup are clean Adjust the gain for WBC 5 part differential parameters in fine mode using for several to 15 venous blood samples from different healthy people which are 30 minutes to 8 hours after collection WARNING Always wear rubber gloves to protect yourself from infection when handling and measuring blood samples Notes about Blood Samples The anticoagulant may cause the blood samples to be unstable for measurement when measured within 30 minutes after collection For gain adjust
41. the bottom of the sample container containing the polymer microsphere suspensions so that the tip of the sampling nozzle comes near the bottom of the sample container NOTE Do not let the sampling nozzle touch the bottom of the sample container This may prevent aspiration of the polymer microsphere suspensions 7 Press the Count switch The polymer microsphere suspensions is aspirated and measurement starts The MEASURING PARTICLES message appears on the screen and the scattergrams and histograms appear in real time The measurement lasts approximately 40 seconds 8 After finishing the particle test the CV and PEAK values calculated from the scattergram and histogram appear on the screen Service Manual MEK 7222 8 DEMONSTRATION GUIDE PARTICLE TEST 12 FEB O3 14 07 FS histogram BE FS FL SD SD histogram CU 4 8 4 5 0 8 peak 58 93 251 OK GAIN OUTPUT MEASURE _ Check the CV and PEAK of FS and FL Scattergram 9 Check that CV and PEAK values are within the following range FS CV 7 0 or less PEAK 61 3 FL CV 7 0 or less PEAK 90 3 NOTE There is no need to check CV and PEAK values of SD If the CV value of FS or FL is more than 7 the flow cell position must be adjusted Refer to the Adjusting the Flow Cell Unit Position section If the PEAK value of FS or FL is outside the above range the gain for WBC 5 part differential measurement must be adjusted Press
42. the hematology analyzer reverse the above procedure Service Manual MEK 7222 4 31 4 DISASSEMBLY AND ASSEMBLY Replacing the AMP CONTROL Board 1 If there are different settings from the default settings on the CALIBRATION and SETTINGS screens write down these settings 2 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Remove the rear cover according to the procedure described in Removing the Rear Cover Including the Power Supply Unit 5 Disconnect the 9 connection cables from the AMP CONTROL board 6 Remove the 3 screws which secure the AMP CONTROL board to the chassis Remove the board from the chassis UT jill MT mL 7 Attach the new AMP CONTROL board to the original position with the 3 screws 4 32 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 8 Connect the 9 connection cables to the AMP CONTROL board 9 Put the rear cover and top cover back to the original positions 10 Turn on the main power switch on the rear panel 11 Continue pressing the Reset key Clean key and Dispense Open key on the front panel press the Power key on the panel release the Power key and release t
43. ust Amo updated after the sample is registered on the peak list The gain adjustment is performed using this mean value You can check if the obtained result is optimum on the PEAK LIST screen before adjusting gain For details on the PEAK LIST refer to the operator s manual 5 Press the AUTO key after finishing registration The ADJUST DIFF GAINS message appears GAIN FS 72 gt 62 FL 62 gt 61 SD 20 30 ADJUST DIFF GAINS Ge NO Qe v 6 Press the YES key to change diff gains The peak list of the registered blood 12 FEB 03 15 41 BLOOD NO DATA FOUND AUTO DIFF GAIN um 200 000 samples is initialized MO PEAK N AIM AVE GAIN FS 62 FL 61 SD 30 AID Service Manual MEK 7222 8 19 8 DEMONSTRATION GUIDE Checking the Gain Adjustment Count from several to about 15 human blood samples from different healthy people which are 30 minutes to 8 hours after collection Display the AUTO DIFF GAIN screen MENU gt SETTINGS SENS amp THRESHOLD AUTO DIFF GAIN Register the measured samples Check that the scattergram and MO are optimum Check the scattergram in each measured data Press the scattergram display area to change the scattergram between MAIN NE EO and MO BA Scattergram check points a The scattergrams are oval in shape and not touching the division line b The scattergrams are in the MO LY and NE area on the left scattergram c There a
44. 0 Reference of the operator s manual 3 1 3 BOARD UNIT DESCRIPTION The diluter unit aspirates a whole blood sample and dilutes it 200 times for WBC HGB and 40 000 times 200 x 200 for RBC PLT with diluent The diluter unit supplies diluent to all the fluid paths There are two pump units One is used for counting the blood cells and measuring the hemoglobin The other is used for WBC 5 part differential measurement The pump unit generates pressure to aspirate the blood sample and drain the waste fluid 3 2 Service Manual MEK 7222 Triple Pump Unit Sampler Unit Service Manual MEK 7222 3 BOARD UNIT DESCRIPTION The triple pump unit aspirates a constant volume of diluent or one of two types of the hemolysing reagents and dispenses the diluent or hemolysing reagent to the specified fluid path The sampler unit aspirates a whole blood sample with the WBC sampling nozzle moves it to the sub bath of the WBC measurement bath and dispenses the aspirated sample and diluent from the diluter unit to the sub bath so that the sample is diluted 200 times in the sub bath The sampler unit aspirates the 200 times diluted sample with the RBC sampling nozzle moves it to the sub bath of the RBC measurement bath and dispenses the 200 times diluted sample and diluent from the diluter unit to the sub bath so that the sample is diluted 40 000 times in the sub bath 3 3 3 BOARD UNIT DESCRIPTION Power Supply
45. 18 Service Manual MEK 7222 Section 6 Maintenance To be Replaced PeriodicallY cs coccion 6 1 Periodic Maintenance Check Procedure cooooccconnocccconoccnononcnonononcnnonn cnn nano nn nano cnn nonnnnnnnnnn canas 6 1 Preparation E 6 1 A EE EE 6 1 Safety cette 6 1 ROA GOING ccc ioo tree zo tex ut temor a be ve ned v EE vns AE ue vu dada 6 1 AA eene nnne nennen nnne nnne nennen nennen 6 2 SENSOR MONITOR SCIGGTn rii tuuc ede ete da 6 3 CIRCUIT CHECK Screen It 6 3 Background To e T 6 4 Measuring the Polymer Microsphere Suspensions cococccocccccoccccconancnconancnnnana canina canon 6 4 Measuring the MEK 5DN Hematorogy Control oooconcoccccnoccccconccaconancnnonancanannn cananea canon 6 4 Current Calibration Coefficients sissien aeea nr 6 4 New Calibration Coeffisients usiessa enisinia nennen a iai nennen 6 4 Software VeriMed 6 5 Checking the Operations cosa Ip D bei lit el AN 6 5 Built in Printer Unit Operation When the printer is installed 6 5 External Printer Unit Operation When the external printer is connected 6 6 Bar Code Reader Operation WHen the bar code reader is installed 6 6 Oui KM DE 6 6 Displaying Operation History Screen siisii anina ainan nnne 6 7 Each 6 7 Displaying the OPERATION HISTORY Screen ccciccoccconocccncnnocacinnnnononancannnnn canina canos 6 7 Checking Cleaning or
46. 3 Test point for output waveform from the peak hold circuit for SD channel 3 TP101 PKD Test point for peak detection pulse of 5 part differential WBC TP102 ANA1 THL Test point for threshold level of 5 part differential WBC TP103 THP Test point for threshold pulse of 5 part differential WBC TP111 Test point for operation check of 5 part differential WBC A D BUSY TP112 Test point for operation check of 5 part differential WBC IRQICK TP113 Test point for operation check of 5 part differential WBC IRQIRS TP114 Test point for operation check of 5 part differential WBC IRQIEN D178 POWER LED for status indication of power supply to this board When the power is supplied to the board this LED is lit D251 CARD IN LED for status indication of memory card insertion When the card is inserted into the slot of the instrument this LED is lit SW061 RESET Reset switch for this board Service Manual MEK 7222 Service Manual MEK 7222 7 Test Point Variable Resistor LED and Switch on Board TP011 5V 5V DC terminal for digital circuit TP012 ED Ground terminal for digital circuit TP013 12V 12 V DC terminal for valve control TP014 E12 Ground terminal for valve control TP015 A12V 12 V DC terminal for analog circuit TP016 EA 12 Ground terminal for 12 V DC A12V supplied analog circuit TP017 Vu Supply voltage Vu for motor control TP018 Eu Ground terminal for motor control TP091
47. 3 TP012 TP011 27V 27 V DC terminal for LCD TP013 36V 36 V DC terminal for analog circuit TP014 15V 15 V DC terminal for analog circuit TPOIS EA Ground terminal for analog circuit TP016 15V 15 V DC terminal for analog circuit TPO17 A12V 12 V DC for analog circuit TPO18 EA12 Ground terminal for 12 V DC use analog circuit TPO19 Vu Supply voltage Vu terminal for motor control TP0110 12V 12 V DC terminal for valve control TPO112 5V 5 V DC terminal for digital circuit TP0113 3 3V 3 3 V DC terminal for digital circuit TP0114 ED Ground terminal for digital circuit TPO115 Vcc Supply voltage Vcc terminal for secondary power circuit control TP0116 Vp Supply voltage Vp terminal for optional built in type printer unit TP0117 Ep Ground terminal for optional built in type printer unit TP0118 E12 Ground terminal for valve control TP0111 Eu Ground terminal for motor control TP021 POWER ON Test point for power off control signal D017 Vcc LED for supply voltage check for secondary power circuit control When the voltage is supplied to the circuit this LED is lit D0110 5V Test point for 5 V DC for digital circuit When the 5 V DC is supplied to the digital circuit this LED is lit D0111 Vu LED for check of supply voltage for motor control When the voltage is supplied to the motor control circuit this LED is lit Service Manual MEK 7222 7 Test Po
48. 4 D025 E oo M RES oo n LA KOHDEN A e 05 A DO11 STB LED for operation check of standby position sensor on the X axis When the sensor is turned on the LED is lit D012 SEN1 LED for operation check of the position sensor 1 on the X axis When the sensor is turned on the LED is lit D013 SEN2 LED for operation check of the position sensor 2 on the X axis When the sensor is turned on the LED is lit D014 HIGH LED for operation check of the high position When the sensor is turned on this LED is lit D015 MID LED for operation check of the middle position When the sensor is turned on this LED is lit D016 LOW LED for operation check of the low position When the sensor is turned on this LED is lit D017 MSMV LED for operation check of the valve When the valve is turned on this LED is lit D024 MFLENCD Reserved LED for operation check of the position encorder for MA 720V flow cell adjuster When the encoder is turned on this LED is lit D025 MFLORG LED for operation check of the original position sensor for MA 720V flow cell adjuster When the sensor is turned on this LED is lit ZANIHON KOHDEN 150 0225 74 8 y e A e ur ee E38 PES 000108 AA G There is no test point Service Manual MEK 7222 7 Test Point Variable Resistor LED and Switch on Board KEY DISPLAY board VRO11 UT 7168 UT 7167 D018 D011 al sicura SWO11 i mp d ar e mapane ao o SWO012 D012
49. 601 E e815 010 6804 9531 fE A 010 6804 7366 F NAE PINTADAS 371 375 5 teach eae 1809 815 020 8761 2726 fE A 020 8761 1135 RIB HE Sb DEAK ARCH 20 Bis 024 2383 2557 fE 024 2340 2565 RESIS EXerD NIBPS E 17 S LAE 420 E Ais 028 8779 6330 f amp 028 8773 6236 EXA EXDXDBRRI 1087 S HRAB 413 E 815 027 8360 2368 fE A 027 8360 2478 NIHON KOHDEN AMERICA INC 90 Icon Street Foothill Ranch CA 92610 USA Phone 1 949 580 1555 Fax 1 949 580 1550 EC REP European Representative NIHON KOHDEN EUROPE GmbH Raiffeisenstrasse 10 D 61191 Rosbach Germany Phone 49 6003 827 0 Fax 49 6003 827 599 NIHON KOHDEN ITALIA S r l Via San Tomaso 78 1 24121 Bergamo Italy Phone 39 035 219 543 Fax 39 035 232 546 NIHON KOHDEN IBERICA S L C Ulises 75A E 28043 Madrid Spain Phone 34 917 161 080 Fax 34 913 004 676 NIHON KOHDEN SINGAPORE PTE LTD 70 Shenton Way 414 05 Marina House Singapore 079118 Phone 65 6224 6700 Fax 65 6224 6216 The model and serial number of your instrument are identified on the rear or bottom of the unit Write the model and serial number in the spaces provided below Whenever you call your distributor concerning this instrument mention these two pieces of information for quick and accurate service Model Your Distributor Serial number PRINTED IN JAPAN
50. AMP CONTROL board with a new one Replace the DRIVER board with a new one E 141 CAP PIERCE INITIALIZE The cap pierce unit does not perform the Replace the cap pierce unit with a ERROR initialization new one E 142 CAP PIERCE RISING The cap pierce unit does not perform the Replace the DRIVER board with a ERROR upward movement new one E 144 CAP PIERCE OPERATION ERROR E 145 TUBE HOLDER OPERATION ERROR E 146 CAP PIERCE RINSING ERROR The cap pierce unit opens the tube holder door during measurement The cap pierce unit does not open the tube holder door when the Dispense Open key is pressed before or after measurement The cap pierce unit does not move to the position of the rinse unit Check that the blood sample tube is properly set in the tube holder Replace the cap pierce unit with a new one Check that a blood sample tube is properly set in the tube holder Replace the cap pierce unit with a new one Replace the cap pierce unit with a new one Replace the DRIVER board with a new one Service Manual MEK 7222 2 5 2 TROUBLESHOOTING Inaccurate Counting and Other Problems If operation is not accurate after attempting the countermeasure in the previous sections check the causes according to the following table Problem Possible Cause Criteria Countermeasure Insufficient grounding Make sure the ground is suffic
51. ATION 5 16 Service Manual MEK 7222 5 Adjustment 2 Follow the instructions on the screen to calibrate the screen SCREEN CALI BRAT I ON f the mark NOTE Do not use a sharp object to press the mark Use your finger After calibration ins completed the screen returns to the READY screen Adjusting Liquid Sensor Output Voltages VRO11 DILUENT VR012 HEMORYNAC 5 VR013 HEMORYNAC 3 VRO14 SHEATH 1 Open the front cover according to the procedure described in Opening the Front Cover of Section 4 Disassembly and Assembly 2 Disconnect the diluent detergent and reagent tubes other than the waste fluid tube from the hematology analyzer 3 Press the Clean key on the front panel to drain all fluid from the hematology analyzer Alarms such as NO DILUENT appear on the screen Check that there is no fluid in the fluid path of the hematology analyzer 5 Press the OTHER key on the MENU screen The OTHER screen appears Service Manual MEK 7222 5 17 5 Adjustment 6 Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen which displays each sensor output voltage appears 7 Adjust the following variable resistors so that the sensor output voltages SHEATH DILUENT HEMOLYNAC 5 and HEMOLYNAC 3 displayed on the screen are 3 5 V Adjusting Contrast of LCD Turn the screen brightness control to obtain the optimal contrast of the screen Screen brightness control 5
52. BC measuring unit with a new one Replace the DRIVER board with a new one Replace the triple pump unit with a E 121 A D CIRCUIT ERROR E 122 CHECK SETTINGS E 122 MEMORY ERROR The A to D converter on the AMP CONTROL board has a failure The backup data is inappropriately changed ERROR initialization new one E 082 TRIPLE PUMP ERROR The triple pump unit does not work Replace the DRIVER board with a new one E 101 WASTE PUMPI ERROR The pump unit does not drain the waste Replace the pump unit with a new fluid from the measurement bath one E 102 WASTE PUMP2 ERROR The pump unit does not drain the waste Replace the DRIVER board with a fluid from the mixing chamber new one E 103 PRESSURE SENSOR The output voltage from the pressure Replace the SHEATH board with a ERROR sensor on the SHEATH board is out of the new one acceptable range Replace the sheath unit with a new one Replace the AMP CONTROL board with a new one Re enter the calibration coefficient and all other settings Then sample counting can be performed If the error still occurs replace the back up battery with a new one E 124 CIRCUIT ERROR CBC E 125 CIRCUIT ERROR DIFF There is a circuit error in the CBC measuring unit or DRIVER board There is a circuit error on the AMP CONTROL board or DRIVER board Replace the CBC measuring unit with a new one Replace the DRIVER board with a new one Replace the
53. BLY 4 14 5 Disconnect the white tube joint and black tube joint from the diluter unit Black tube joint White tube joint 6 Pull the diluter unit upward Carefully separate 1t from the hematology analyzer chassis and disconnect the cable from the diluter unit Completely remove the diluter unit from the chassis To assemble the hematology analyzer reverse the above procedure CAUTION in Assembling the Hematology Analyzer Check that all the tube joints are firmly connected to the original positions Otherwise fluid may leak Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Removing the Sampler Unit 4 Service Manual MEK 7222 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer Open the front cover according to the procedure described in Opening the Front Cover Remove the top cover according to the procedure described in Removing the Top Cover Nozzle mover Move the sampling nozzle support plate upward and move the nozzle mover leftward as shown below so that you can remove the 4 screws which secure the sampler unit to the chassis Sampling nozzle support plate Remove the 4 screws which secure the sampler unit to the chassis Release the 3 tube ties and remove the tube joint as shown below i 4 15 4 DISASSEMBLY AND ASSEMBLY 7 Pull the sampler unit upward until the hooks at bo
54. COMPOSITION srt Rr P 1 12 Standard Composition mic ir A sie Ae delat 1 12 OPUONS surcar 1 14 Section 2 A Troubleshooting soii ati 2C 1 General veian id ii dida R 2 1 Eiro Messages ati a de rob 22 System Error Messages oooocccoooccccononcnconnoconononcnnonnnnn none nr rra nn r aran nn nrannn rra nn nn rn rare narran rn rnnnaninenns 25 Inaccurate Counting and Other Problems oococcncccccnnccccconocancnancnonannnccnonn cnn cnnnn conan n conan cnn nana 26 Section3 Board Unit DeScriptiOO ooomcnccconoonecccnccccccnnnonorrrrnnnnaanannnnnos 3C 1 esed CE mU m M 3 1 Service Manual MEK 7222 C 1 CONTENTS Section4 C 2 AAA EE 3 2 Pump UMi e 3 2 Tipe PUMP UNI E 3 3 Sampler UMI dete ii ii 3 3 Power Supply Unit and POWER Board cccccoocccinoccccnnoccnconnnccninnnnonannncnconn cnn none n nc iaaa nnns 3 4 InlevOutlstUnit aa ci E E E det 3 4 lg ES 3 5 Front Panel nit otto oi dd 3 5 Optional Printer Unit WA 720VK cocncccnnnccccononcccnnnonnncnnnnanonnn nn cnn nennen rana 3 6 Rinse Unit and Switch Assembly cocccconnccccncccccnconcccnnnnnccnnnnncnnnnnn cnn nnnn cn cnn nc nn enne nnne nnne nnns 3 6 EaserOpticaiUn lacoste line 3 7 do Aem 3 7 AMP CONTROL Boat P nce 3 7 DRIVER Board HR CT P EET NE 3 8 Optional Cap Pierce Unit MS 724V issie adana aaaea d nennen nennen
55. D and Switch on Board PRE AMP board SWO011 Swo012 TP022 TPO21 TPO11 VRO21 D023 D024 D025 D026 D027 D028 TPO11 EA Ground terminal for analog circuit TP021 C ADJ Test point for circuit check operation TP022 CIN Test point for blood cell pulse operation VRO21 ELE VR Variable resistor for adjustment of voltage between the electrodes D023 HGB LDE LED for operation check of the HGB LED When the HGB LED is lit this LED is also lit D024 WBC AMP LED for operation check of WBC measurement circuit selection When the circuit is selected this LED is lit D025 WBC VOLT LED for operation check of WBC high voltage circuit selection When the circuit is selected this LED is lit D026 RBC VOLT LED for operation check of RBC high voltage circuit selection When the circuit is selected this LED is lit D027 RBC AMP LED for operation check of RBC measurement circuit selection When the circuit is selected this LED is lit D028 CAL ON LED for operation check of circuit check pulse selection When the circuit check pulse is selected this LED is lit SWOI1 MC ADJ 1 Switch for compensation of the manometer This switch is used for the second digit of the two digit compensation value SWOI2 MC ADJ 2 Switch for compensation of the manometer This switch is used for the first digit of the two digit compensation value Service Manual MEK 7222 7 1 7 Test Point Variable Resistor LED and Switch
56. DN hematology control three times Refer to Measuring Calibrator in Section 7 Calibration of the operator s manual Service Manual MEK 7222 Software Version Checking the Operations Built in Printer Unit Operation When the printer is installed Service Manual MEK 7222 6 MAINTENANCE 3 Calibrate the hematology analyzer by entering the new calibration coefficient or measured data Refer to Calibration in Venous Blood Mode or Calibration in Capillary Blood Mode in Section 7 Calibration of the operator s manual NOTE Before entering the new calibration coefficient or measured data ask the customer if you can change the setting Some customers calibrate the hematology analyzer for data consistency with other manufacturers hematology analyzers Press the OTHER key on the MENU screen The OTHER screen appears 2 Press the OPERATION HIST key on the OTHER screen The OPERATION HISTORY screen appears It displays the software version total operating time total number of counts and number of counts that filters and pump tubes are used Refer to Displaying Operation History Screen in this section 3 Write down the software version on the check sheet Touch screen Check the touch screen function Refer to Calibrating Touch Screen in this section Date and time Check the date and time settings Refer to Setting Date and Time in Section 3 Changing Settings of
57. Delivery Date Person in charge of installation Checking Items OK Not OK Preparing the hematology analyzer 1 Connect tubes 2 Connect power cord and grounding lead 3 Turn laser switch on 4 Turn power on Date and Time Check and Cleaning 1 Check date and time settings 2 Clean fluid path in the hematology analyzer Adjusting gain and measuring background noise Item Value Standard range FS CV 1 096 1 Count polymer microsphere suspensions FS peak 6143 FL CV lt 7 0 FL peak 903 2 Adjust flow cell unit position CV 96 of FS and FL are lt 7 0 m FS peak 6143 3 Adjust FS and FL gain in rough mode FL peak 903 WBC lt 0 2x107 uL RBC lt 0 05x10 UL 4 Measure background noise HGB lt 0 1 g dL PLT lt 10x10 uL TOC lt 100 5 Adjust FS FL and SD gain in fine mode MO peak AVE AIM FS 186 to 192 6 Check the gain adjustment FL 47 to 51 SD 21 to 23 Calibration 1 Measure MEK 3DN hematology control Parameter on coefficient WBC 2 Calibrate CBC parameters RBC HGB HCT PLT 3 Calibrate WBC 5 part differential parameters Checking data 1 Check with MEK 5D hematology control 2 Check with human blood samples 8 34 Service Manual MEK 7222 quel Manufacturer NIHON KOHDEN CORPORATION 1 31 4 Nishiochiai Shinjuku ku Tokyo 161 8560 Japan Phone 81 3 5996 8036 Fax 81 3 5996 8100 ESAS He KA 169 5 SHAS 3008 38 Eid 021 6469 9016 f amp 021 6486 7218 AG HEU IR kK StL 3S HBAS
58. EBS 14 07 CU 4 8 4 5 8 0 PEAK 58 93 251 L9 Gk mur tE If the CV of FS and FL are below 7 go to Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions section If the CV of ES and FL are above 7 repeat the procedure to clean the flow cell and measure polymer microsphere suspensions again Then proceed to next step 7 Press the ADJUST FLOWCELL key on the OPERATIONS screen to display the ADJUST FLOWCELL screen ADJUST FLOWCELL 14 FEB B3 15 21 MANUAL ADJUST Service Manual MEK 7222 5 7 5 Adjustment 8 Press the MANUAL ADJUST key MANUAL ADJUSTMENT 12 FEB OS 14 04 FS 4 6 FL B C 60 COUNT 6595 bs B POSITION CVE 5 0 4 5 NOW START gt PEAK 58 92 4937 5000 2 guru reis 9 Press the MEAS key The PRESS COUNT KEY TO START message appears COUNT 0 ha RE POSITION Cy 0 0 0 0 NOW START gt PEAK 0 0 5000 5000 _CANCEL PRESS COUNT KEY TO START 10 Count the polymer microsphere suspensions again Refer to step 6 he Press the gt or lt key to adjust the flow cell position The histogram is automatically redrawn Adjust the flow cell until the CV of ES and FL is minimum and the peak is maximum MANUAL ADJUSTMENT 12 FEB 83 13 29 FS 41 FL 60 GUNT 707 Adjust flow cell osition For clearing and redrawing Re HE POSITION p scattergrams without OV 23 8 13 3 NOW
59. ENERAL Laser Optical Unit Flow Cell Unit MO Preamp Board Set MO FS PREAMP Board MO FL PREAMP Board MO SD PREAMP Board MO LD DRIVER Board Pump Unit For CBC Pump Unit For WBC 5 part Triple Pump Unit TRIPLE PUMP Board 3 way Valve 3 way Valve 3 way Valve Rinse Unit and Switch Assembly Sampler Unit SAMPLER X Board SAMPLER Z Board 3 way Valve Front Panel Unit Front Panel Board Set KEY DISPLAY Board VOLUME Board Power Supply Unit AMP CONTROL Board DRIVER Board POWER Board 1 GENERAL Options WA 720VK UT 7136 UT 7123 UT 7124 MS 721V UT 7189 MT 7190 MT 7191 XP 513V WA 460V Printer unit Printer Board Set PRINTER INTERFACE Board PRINTER KEY Board Cap Pierce Unit Cap Pierce Board Set CAP PIERCE 1 Board CAP PIERCE 2 Board 3 way Valve Card Printer Service Manual MEK 7222 Section 2 Troubleshooting erc P 2 1 Error Messages M E 2 2 System Error M6SSAGES ivive cerei ee dE EE ca ou Pe Ea RU Eu d ERR pu Duda 2 5 Inaccurate Counting and Other Problems ssssssseeeeeeeeeeneeenneeen nennen 2 6 Service Manual MEK 7222 2C 1 2 TROUBLESHOOTING General If a problem occurs the built in error detection function displays an error message with an alarm sound The tables in the next sections show the types of error message their causes and their countermeasures Use the
60. ICE POICY EU 1 2 Specifications sinense RR 1 3 Measured Parameters Ranges and Reproducibility to Specimen from Venous soo Eaa 1 3 Detection Method estados ase renes eden ra Eva EORR ewe d aues aE 1 8 Standardization Analysis Method sssssseeeeeneeeennee 1 3 Interference Substances iraisser aiana a aaia 1 4 Dilution Ratio A o o UE O aaa a E a a ade 1 6 COUNUNO TIMO saaa e E T RE E 1 6 DDISPIAY sucia 1 6 Data SUD Mtr CT 1 6 Environmental Conditions oooooccccconnoocccnccconoonnnnnnncnnnonnnnnnncnonononnnnncnnnnnnnnnnncnnnns 1 6 Power Requirements iii 1 6 Dimensions and Welght 5 ebraica eer eben Ene x en See sa ERR qua 1 7 Electromagnetic Compatibility 1 7 Salely sce n e MEME LUE 1 7 Panel DESCrPlONS er c EMI UE 1 8 Front Panel soe ote tese ox e terne A a 1 8 Right Side Panel AP PRPUNUoo PP nennen nennen nennen nnn eE nn 1 9 Rear Panel iii 1 10 Inside II 1 11 COMPOSITION UM a iaa 1 12 Standard Compositor il 1 12 OPINAS dota 1 14 Service Manual MEK 7222 1C 1 Introduction Service Manual MEK 7222 1 GENERAL CAUTION To maintain the instrument in normal condition the user must perform the periodic maintenance Refer to Maintenance of the operator s manual This service manual provides useful information to qualified service personnel to understand troubleshoot
61. Items for Make sure that you have the following necessary items for the demonstration Demonstration These are provided with the hematology analyzer The number in the parentheses indicates the necessary quantity 0 Waste tube 1 CLEANAC tube 1 18 L cap 3 Power cord 1 Ground lead 1 ISOTONAC tube 1 18 L diluent tube assy 1 18 L diluent tube assy Cleanac tube 8 for Cleanac tube assy 8 WASTE 1 CLEANAC8 3 1 8222 2 Qr 1 QU Hemolynac3 cap 2 Hemolynace3 tube assy 1 Hemolynace5 tube assy 1 Waste container 1 ra Laser key 2 Manuals 1 set 8 2 Service Manual MEK 7222 Service Manual MEK 7222 8 DEMONSTRATION GUIDE The following items are also required Diluent ISOTONAC 3 MEK 640 Detergent CLEANAC MEK 520 Detergent CLEANAC 3 MEK 620 Hemolysing reagent HEMOLYNAC 3 MEK 660 or HEMOLYNAC 3N MEK 680 Hemolysing reagent HEMOLYNAC 5 MEK 910 7 um polymer microsphere suspensions YZ 0194 supply code no T905 Hematology control MEK 3DN Hematology control MEK 5DN H L Sample containers Wiping cloth tissue papers Rubber or latex gloves Distilled water Cleaning bottle kit YZ 0252 15 blood samples from different healthy people 8 3 8 DEMONSTRATION GUIDE Preparing the Hematology Analyzer Installation Flowchart Connecting Tubes 8 4 Connect the diluent detergent hemolysing reagent and waste containers to the hematology analyzer Connect the power cord a
62. K are res MEAS key Service Manual MEK 7222 8 DEMONSTRATION GUIDE 3 Out of diluent or detergent alarm are not displayed If diluent or detergent runs out bubbles enter the flow cell If the out of diluent or detergent alarm appears replace diluent or detergent and clean the fluid path in the hematology analyzer by pressing the Clean key on the front panel or CLEAN FLOWCELL key on the OPERATIONS screen 4 The polymer microsphere suspensions are old or coagulated Perform the particle test after getting new polymer microsphere suspensions and adjust the flow cell unit position only when the CV value of the particle test greatly exceeds 7 Adjusting the Flow Cell Unit Position The flow cell unit must be adjusted when the laser does not scatter exactly on the sample flowing inside the flow cell Check if the laser scatters on the sample by the particle test NOTE When the flow cell is clogged with dust it cannot be adjusted correctly Clean the fluid path in the hematology analyzer by pressing the Clean key on the front panel or CLEAN FLOWCELL key on the OPERATIONS screen before adjusting the flow cell unit position 1 Prepare 1 mL of polymer microsphere suspensions in a sample container 2 Display the MANUAL ADJUSTMENT screen MENU gt OPERATIONS gt ADJUST FLOWCELL MANUAL ADJUST 3 Press the MEAS key The PRESS COUNT KEY TO START message appears and the hematology analyzer is ready to cou
63. MBLY AND ASSEMBLY 5 Gently pull the flow cell adjuster upper part upward and separate it from the laser optical unit 6 Disconnect the flow cell adjuster cable at the sampler unit 7 Remove the adjuster pin from the flow cell unit Refer to Removing the Laser Optical Unit To assemble the hematology analyzer reverse the above procedure Service Manual MEK 7222 4 25 4 DISASSEMBLY AND ASSEMBLY Removing the Laser Optical Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Remove the rear cover including the power supply unit according to the procedure described in Removing the Rear Cover Including the Power Supply Unit 4 Remove the 4 nuts M5 which secure the laser optical unit to the chassis Use a suitable hex socket driver 5 Remove the 2 screws which secure the flow cell unit to the holder 2 screws Holder 6 Move the holder leftward and downward and remove it from the laser optical unit 4 26 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 7 Disconnect the 2 PharMed tubes from the single manifold Manifold 2 PharMed tubes 9 Remove the tube joint No 4 Release the 3 tube ties Disconnect the cable as shown below Remove the laser optical unit and flow cell adjuster from the ch
64. N NE EO and MO BA when touched MEAS key in PARTICLE mode 62 ED 61 VIY REDRAW key in BLOOD and oe Qm 7 0 EE A MES CALC ER 2 Press the sample type key to select PARTICLE The scattergram and the peak for the polymer microsphere suspensions appear on the screen 3 Press the CALC key The optimal FS value calculated by the current NOW and target AIM peak values appear next to VALUE 4 Press the ENTER key to register the value to the FS box The value now appears next to FS and the cursor moves to FL 5 Repeat steps 3 and 4 to register the optimal FL value Service Manual MEK 7222 5 9 5 Adjustment 5 10 NOTE In PARTICLE mode SD gain cannot be adjusted SD gain is adjusted in fine mode by using human blood samples 6 Press the MEAS key to return to the PARTICLE TEST screen and count the polymer microsphere suspensions again 7 Tf the peak is not optimum repeat the procedure in the Counting the Polymer Microsphere Suspensions and Adjusting the Flow Cell Position section until you obtain the optimum value NOTE The gain adjusted by the polymer microsphere suspensions may not be the optimum gain for human blood Every time you adjust the gain roughly by the polymer microsphere suspensions adjust the gain in fine mode by using human blood samples Adjusting Gain for WBC 5 Part Differential Measurement Finely by Human Blood Sample NOTE Adjust the gain in rough mode using pol
65. Open key While pressing the Reset key press the Capillary blood mode key on the front panel The TOUCH SCREEN CALIBRATION screen appears Adjust the touch screen properly Refer to Calibrating Touch Screen in Section 9 Maintenance of the operator s manual Initialize the settings according to Initializing Settings in Section 3 Changing Settings of the operator s manual Enter the settings which you wrote down in step 1 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Removing the Rear Cover Including the Power Supply Unit 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Loosen the 2 screws at the bottom of the rear cover 4 Remove the 7 screws which secure the rear cover to the hematology analyzer chassis 5 Pull the rear cover including the power supply unit upward Carefully separate the rear cover from the hematology analyzer chassis and disconnect the 4 cables at the POWER board Completely remove the rear cover from the chassis To assemble the hematology analyzer reverse the above procedure Service Manual MEK 7222 4 11 4 DISASSEMBLY AND ASSEMBLY Removing the CBC Measuring
66. PERATION EQUIPMENT types classification Indoor stationary EQUIPMENT Pollution Degree 2 EQUIPMENT Requirements for marking of IN VITRO DIAGNOSTIC instruments EN1658 1996 Service Manual MEK 7222 1 7 1 GENERAL Panel Descriptions Front Panel VA NIHON KOHDEN GeutacE asd aJ 2 16 3 17 T 18 5 6 19 7 8 9 20 10 11 12 13 No Name Description 1 LCD display Displays various messages measured data and touch screen keys 2 Power lamp Lights when the main power switch on the rear panel and power key on the front panel are turned on 3 Main power lamp Lights when the main power switch on the rear panel is tuned on 4 Laser lamp Lights when the laser switch on the right side panel is turned on Turns the hematology analyzer power on or off when the main power switch on the rear panel is 5 Power key turned on When the power is turned on priming and self check are automatically performed and the READY screen appears Stops operation when pressed during operation Returns to the READY screen when pressed while 6 Reset key changing settings Use this key only when an error occurs Cleans the fluid path aperture and manometer with detergent Automatically primes after cleaning the 7 Cleankey fluid path Press this key when clogging occurs the manometer becomes dirty or bubbles occur in the manometer Auto print mode i d 8 Das L
67. PERATION screen to perform the strong cleaning 2 2 Service Manual MEK 7222 Error Messages Possible Cause Criteria 2 TROUBLESHOOTING Countermeasure A 062 HGB VOLTAGE HIGH A 063 HGB CIRCUIT ERROR A 064 HGB VOLTAGE LOW HGB sensor output adjustment error Error in the HGB circuit The WBC measurement bath is dirty or the CBC measuring unit has a failure Adjust the HGB sensor output Refer to Adjusting HGB Sensor Output Voltage in Section 5 Adjustment Replace the CBC measuring unit with a new one if the internal connections have no problem and the message still appears after the adjustment Replace the CBC measuring unit with a new one 1f the internal connections have no problem and the message still appears after the adjustment Check the HGB sensor output and adjust it after cleaning the measurement bath Refer to Adjusting HGB Sensor Output Voltage in Section 5 Adjustment Replace the CBC measuring unit with a new one if the internal connections have no problem and the message still appears after the adjustment A 070 DOOR IS OPEN The hematology analyzer does not operate because the door of the MS 721V cap pierce unit is open Close the door tube holder A 071 TUBE IS CONTAINED A 081 LASER OFF Open mode can not be selected because the sample tube is set in the MS 721V cap pierce unit The laser switch is not set to on Remove the sample tube a
68. Removing the Triple Pump Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Disconnect the 8 tube joints No 18 to No 25 19 T G ll 23 Y 36 al 21 4 Remove the 2 black screws which secure the triple pump unit to the chassis 5 Disconnect the black tube joint No 12 connected to the inlet outlet unit as shown below 12 6 Carefully pull the triple pump unit frontward Disconnect the cable from the triple pump unit Remove the triple pump unit from the chassis To assemble the hematology analyzer reverse the above procedure 4 18 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Removing the WA 720VK Printer Unit Option Service Manual MEK 7222 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Release the 2 cable ties which bind the flat cable of the printer unit Cable ties 4 Remove the 4 screws which secure the printer unit to the front cover 4 19 4 DISASSEMBLY AND ASSEMBLY 5 Disconnect
69. SERVICE MANUAL MEK 7222J MEK 7222K Cellitac E AUTOMATED HEMATOLOGY ANALYZER MEK 7222 Va NIHON KOHDEN 0634 001896A aeujf amp ugno Model MEK 7222J K Manual code no 0634 001896A Reader Comment Card We welcome your comments about this manual Your comments and suggestions help us improve our manuals Please circle the number for each of the following statements corresponding to your evaluation and add comments in the space provided Fax or send your completed comment card to Fax 81 3 5996 8100 International Div Sales Promotion Section Nihon Kohden Corp 1 31 4 Nishiochiai Shinjuku ku Tokyo 161 8560 Japan Strongly Agree Neutral Disagree Strongly Agree Disagree This manual is organized 1 2 3 4 5 can find the information want 1 2 3 4 5 The information is accurate 1 2 3 4 5 can understand the instructions 1 2 3 4 5 The illustrations are appropriate and helpful 1 2 3 4 5 The manual length is appropriate 1 2 3 4 5 Comments Thank you for your cooperation We appreciate it very much Name Occupation Position Hospital Company Address Phone CONTENTS Contents EMG Related CANNON aria aa i Conventions Used in this Manual and Instrument c cccceeeeeeeeeeeeeeeneeeeeeeeeeeseneeeesenaeeeeeaes iii Warning
70. TION screen NOTE After calibration all measured data are automatically deleted 8 25 8 DEMONSTRATION GUIDE Calibration for WBC 5 Part Differential Parameters 8 26 CALIBRATION VENOUS D Check that the calibration coefficients of LY MO EO and BA for WBC 5 part differential parameters are 1000 In the gain adjustment performed in the previous sections WBC 5 part differential parameters are already accurately calibrated Therefore you only need to check the calibration coefficient Display the CALIBRATION VENOUS screen MENU gt CALIBRATION gt VENOUS 14 FEB 3 12 00 ATA CAL 7 3 JL 1000 J quem RBD 4 26 IL 1000 J VALUEL SD 12 7 JE 1000 mem 38 6 IL 1000 eee AED 90 7 MCH 29 9 4 8 MCHC 33 0 2 Press the NEXT key to display the WBC 5 part differential parameters CALIBRATION VEN 14 FEB O3 Check that the calibration coefficients of LY 96 MO EO and BA are 1000 If the calibration coefficients are not 1000 move the cursor to that CAL field and enter 1000 using the numeric keypad Press the ENTER key to register 1000 as the calibration coefficient Service Manual MEK 7222 8 DEMONSTRATION GUIDE Checking the Data Check the following data to confirm that the gain is adjusted and calibration is performed properly a Measure the MEK 5DN L H hematology control to check that the obtained data falls within the acceptable range on the assay sheet
71. Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the 4 black screws which secure the valve shield cover and sampling cup support bracket including the sampling cup Remove them from the hematology analyzer Sampling cup support bracket Valve shield cover 4 Remove the 4 screws which secure the CBC measuring unit to the hematology analyzer chassis Tube tie 4 12 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 6 Disconnect the No 1 No 3 No 4 No 5 and No 8 tube joints 7 Disconnect the 2 flat cables from the CBC measuring unit 8 Remove the CBC measuring unit from the chassis completely To assemble the hematology analyzer reverse the above procedure Removing the Diluter Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Remove the black screw which secures the lower part of the diluter unit Screw at the upper part of the unit is behind the valve Screw at the lower part of the unit 4 Remove the black screw which secures the upper part of the diluter unit Service Manual MEK 7222 4 13 4 DISASSEMBLY AND ASSEM
72. Unit and POWER board AM POWER board The power supply unit has a power transformer and AC inlet socket with main power switch and two fuses The power supply unit transforms the AC line voltage to AC voltages The POWER board converts the AC voltages to the DC supply voltages for the boards motors and valves Inlet Outlet Unit The inlet outlet unit detects the diluent detergent and two different hemolysing reagents These fluids are supplied to the specified fluid path through this unit All waste fluids are drained through this unit 3 4 Service Manual MEK 7222 Sheath Unit Front Panel Unit Service Manual MEK 7222 3 BOARD UNIT DESCRIPTION The sheath unit produces a specific sample for WBC 5 part differential measurement The sample is mixed with the hemolysing reagent and injected into the center of the flow cell The pressurized diluent is injected into the sheath of the flow cell The front panel unit consists of the KEY DISPLAY board and VOLUME board The KEY DISPLAY board has key switches such as Power key and Dispense Open key and LED lamps such as power lamp and operation indicator lamps The numeric graphic and message data are processed on this board to display them on the LCD The touch screen communicates with this board The VOLUME board has a variable resistor for contrast control This front unit has a space for the optional WA 720VK printer unit 3 5 3 BOARD UNIT DESCRIPTION Optional
73. above suggested remedial actions do not solve the problem consult your Nihon Kohden Corporation subsidiary or distributor for additional suggestions This equipment complies with International Standard EN55011 1999 Group 1 Class B Class B EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes Service Manual MEK 7222 i This page is intentionally left blank Service Manual MEK 7222 Conventions Used in this Manual and Instrument Warnings Cautions and Notes Warnings cautions and notes are used in this manual to alert or signal the reader to specific information WARNING A warning alerts the user to the possible injury or death associated with the use or misuse of the instrument CAUTION A caution alerts the user to possible injury or problems with the instrument associated with its use or misuse such as instrument malfunction instrument failure damage to the instrument or damage to other property NOTE A note provides specific information in the form of recommendations prerequirements alternative methods or supplemental information Service Manual MEK 7222 iii Explanations of the Symbols in this Manual and Instrument The following symbols found in this manual instrument bear the respective descriptions as given On panel Symbol Descript
74. alve 5 in the MJ 721V sheath unit When the valve is turned on this LED is lit D096 6 LED for operation check of valve 6 in the MJ 721V sheath unit When the valve is turned on this LED is lit D097 7 LED for operation check of valve 7 in the MJ 721V sheath unit When the valve is turned on this LED is lit D098 8 LED for operation check of valve 8 in the MJ 721V sheath unit When the valve is turned on this LED is lit D099 9 LED for operation check of valve 9 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0910 10 LED for operation check of valve 10 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0911 11 LED for operation check of valve 11 in the MJ 721 V sheath unit When the valve is turned on this LED is lit D0912 12 LED for operation check of valve 12 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0913 13 LED for operation check of valve 13 in the MJ 721V sheath unit When the valve is turned on this LED is lit 7 15 7 Test Point Variable Resistor LED and Switch on Board D0914 14 LED for operation check of valve 14 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0915 15 LED for operation check of valve 15 in the MJ 721V sheath unit When the valve is turned on this LED is lit D0916 16 LED for operation check of valve 16 in the MJ 721V sheath unit When the valve is turned on this
75. anel Unit ooooooocccccncnococcccncnononancnoncnononnncnnnn nono cnn eene nnne 4 20 Removing the Inlet Outlet Unit sisisi irnar sariani nnne nnns enne 4 22 Removing the Flow Cell Adjuster oooonoccnnnnccconnnccccnnnccnconnacnnonnncnnnnnn conan n cn nannn narran n cn nnne nnn 4 24 Removing the Laser Optical Unit esssessssessessseseee eene enne enne enne nennen 4 26 Removing the Sheath Unit 221 11er ie ie a 4 28 Removing the Rinse Unit and Switch Assembly ssssssseeeeeennn 4 31 Replacing the AMP CONTROL Board cocococcnnoccccnocccncononcnonnnnnononnncnnnnnonoconnn nc rana ca nennen 4 32 Replacing the DRIVER Board nicotina 4 33 Replacing the VOLUME Board KEY DISPLAY Board and LCD Unit ssssssss 4 34 Replacing the MS 721V Cap Pierce Unit Option sssssmee 4 36 Service Manual MEK 7222 CONTENTS Section 5 Aumenta 5C 1 Adjusting HGB Sensor Output Voltage sse eene nennen nennen 5 1 Adjusting Upper and Lower Sensor Output Voltage of the Manometer ssss 52 Compensating manometer Volume ooconnoccconocccnnonacaconnncnnonncnnnanonannnnn no nonnn narran oca nenne nennen 5 3 Adjusting Gain for WBC 5 Part Differential Measurement ssssseeeeee 5 4 Counting the Polymer Microsphere Suspensions and Adjusting the Flow Cell Position 0 cccccceceeceeeeeeeeeeeseneeeeeeneeeeeeaeeeeeeaeeese
76. anual MEK 7222 Service Manual MEK 7222 7 Test Point Variable Resistor LED and Switch on Board TPO16 EA12 Ground terminal for 12 V DC supplied to analog circuit TP017 Vp Supply voltage Vp terminal for optional built in printer TPO18 Ep Ground terminal for optional built in printer TPO19 Vcc Supply voltage Vcc for secondary power circuit control TP0110 3 3V 3 3 V DC terminal for digital circuit TP0111 36V 36 V DC terminal for analog circuit TP0112 27V 27 V DC terminal for LCD TP0113 15V 15 V DC terminal for analog circuit TPO114 EA Ground terminal for analog circuit TP0115 15V 15 V DC termial for analog circuit TP021 CIN Test point for blood cell pulses from MC 720V CBC measuring unit TP022 CFIL Test point for output waveform through the low pass filter TP023 RB Test point for output waveform from the Robinson gate circuit TP024 CLOUT Test point for output waveform from baseline stabilizer circuit TP031 PEAK HOLD Test point for output waveform from the peak hold circuit TP032 CANA Test point for output waveform from the gain setting circuit TP033 LOW THR Test point for lower threshold level TP034 HIGH THR Test point for upper threshold level TP061 VLD Test point for laser operation TPO71 ANA1 Test point for output waveform for FS channel 1 from MO 820V laser optical unit TP072 FIL1 Test point for output waveform through the low pass filter for FS chann
77. anual MEK 7222 3C 1 CBC Measuring Unit Service Manual MEK 7222 3 BOARD UNIT DESCRIPTION The CBC measuring unit has two measurement baths and a common manometer Each measurement bath has an aperture cap The aperture size is different for the two baths WBC and RBC PLT Two electrodes are positioned so that the aperture is in between them A constant current flows between the two electrodes When blood cells pass through the aperture it causes electrical resistance and the voltage between the two electrodes varies in proportion to the cell size These voltage pulses for each cell are obtained until a constant volume of diluted sample is aspirated For more details about counting method and hydraulic operation refer to Electric Cell Counting and Principle of Hydraulic Operation in Section 10 Reference of the operator s manual After the chemical processing is applied to the blood sample the CBC measuring unit measures the hemoglobin concentration when a specific wavelength light 540 nm is transmitted through the blood sample and diluent to the HGB sensor The light intensity passed through the blood sample is inversely proportional to the hemoglobin concentration The light intensity passed through diluent is used as reference The HGB sensor converts the light intensity to current A voltage is obtained from the current For more details about chemical processing refer to Hemoglobin Measurement in Section 1
78. assis No 4 Cable NOTE To remove only the laser optical unit separate the flow cell adjuster from the laser optical unit Refer to the Removing the Flow Cell Adjuster To assemble the hematology analyzer reverse the above procedure Service Manual MEK 7222 4 27 4 DISASSEMBLY AND ASSEMBLY Removing the Sheath Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Remove the rear cover including the power supply unit according to the procedure described in Removing the Rear Cover Including the Power Supply Unit 3 Disconnect the 6 tube joints No 16 to No 21 as shown below No 21 No 17 No 16 No 18 Black screw 5 Remove the black screw which secures the lower part of the sheath unit to the chassis 6 Disconnect the 4 black tube joints No 4 No 10 No 13 No 14 and white tube joint from the diluter unit No 10 No 14 No 13 No 4 White tube joint from diluter unit 7 Remove the 2 black screws which secure the upper part of the sheath unit to the chassis 4 28 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 8 Disconnect the 3 cables between the sheath unit and DRIVER board 9 Disconnect the 2 cables at the SHEATH board as shown below 10 Disconnect the tube joint from the manif
79. asurement mode is correct venous blood mode or capillary blood mode Calibration in venous mode must be checked before calibration in capillary mode Perform the measurement for capillary blood calibration in the capillary blood mode by pressing the Capillary mode key on the front panel Prepare the capillary blood samples of the volume you have selected on the CAPILLARY screen on the MEASURE MODE screen For details refer to the operator s manual Display the CALIBRATION MEASURE screen MENU gt CALIBRATION gt CAL MEASURE If there is no calibrator measured data stored in the memory the MEASURE CALIBRATION SAMPLE message appears E0 BAX RBC HGB HET MEASURE CALIBRATION SAMPLE EHE RDW Measure the calibrator The MEASURING message appears on the screen After measurement the measured data appears on the screen Measure the calibrator more than 3 times The screen lists the measured data for each measurement and the mean value of all measurements Up to 5 measurement data can be listed If you measure more than 5 times the oldest data is deleted to store the latest data CALIBRATION MEASURE 14 FEB 03 11 58 ii D oe eee E eS PEL Gey DS 18 7 58 4H 56 9H 46 6 i O 33 1H 28 4H 3 1H 35 2 ncm HEN 1 8 Mean value 4 28 4 23 4 26 4 28 12 12 12 8 12 7 38 5 38 4 38 6 38 5 80 4 30 8 90 6 38 6 24 8 30 0 30 0 29 9 SEXE Seo Sa RR a 13 9 14 0 13 9 13 9 223 223 230 225 8 14 8 14 20 15 8 14
80. at are resistant to lysis may interfere with an accurate LY count MO and MO Large lymphocytes atypical lymphocytes blasts and excessive number of basophils may interfere with an accurate MO count NE and NE Excessive eosinophils metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate NE count and NE EO and EO 2c Abnormal granules may interfere with an accurate EO count BA and BA 4v Immature cell metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate BA count and BA Dilution Ratio e Venous blood Sample volume 55 uL for 22 parameters 30 uL for WBC RBC HGB HCT MCV MCH MCHC and PLT WBC HGB 200 1 RBC PLT 40 000 1 Capillary blood Sample volume 10uL 20 uL WBC HGB 1200 1 600 1 RBC PLT 240 000 1 120 000 1 Counting Time Within 70 s sample from measurement start to data display Display Display 5 5 inch LCD with backlight and touch screen keys Resolution 320 x 240 dots Screen size approx 86 x 115 mm Display contents Numerical data scattergrams histograms measuring conditions alarm message and other messages touch screen keys Data Storage Numerical data for all counted parameters for up to 400 samples and histograms and scattergrams for up to 50 samples Environmental Conditions Storage temperature 20 to 60 C Operating temperature 15 to 30 C Storage humidity 10 to 95 Non condensing Ope
81. ation coefficient Calibration can be performed for the following parameters in venous blood mode and capillary blood mode individually e WBC MCV RDW RBC PLT MPV HGB The MEK 3DN hematology control can be used as a calibrator if it is stored at 2 to 8 C and is used within 3 days after opening For higher accuracy use the calibrator soon after opening HCT is calibrated from RBC and MCV Therefore it is calibrated by calibrating RBC and MCV NOTE Calibration with calibrator can be performed for WBC RBC HGB HCT PLT and MPV but not for WBC differential parameters To calibrate WBC differential parameters refer to the Calibration for WBC 5 Part Differential Parameters section The MEK 5DN hematology control cannot be used as a calibrator MEK 5DN is for accuracy control e MEK 3DN is for accuracy control of the hematology analyzer refined from normal human blood The calibrator contains living cells Handle and store the calibrator properly following the instructions in the assay sheet 8 21 8 DEMONSTRATION GUIDE Measuring the MEK 3DN Hematology Control To perform accurate calibration you need to obtain a mean value of the calibration measurement by measuring the MEK 3DN more than 3 times The mean value of the measured data can be automatically calculated and entered on the CALIBRATION screen Measured data Press OK to return to CALIBRATION screen 8 22 NOTE Check that the me
82. ault compensation value set at our factory is attached to the diluter unit There are two rotary switches on the DILUTER DRIVER board Each rotary switch selects one of 10 terminals The rotary switch for the first digit of the two digit compensation value has 0 2 increment or decrement per terminal The rotary switch for the second digit of the two digit compensation value has 2 increment or decrement per terminal 5 15 5 Adjustment When the HGB and WBC data have 2 decrease with the hematology control after replacing the cylinder block with a new one change the compensation value with the rotary switches so that the HGB and WBC data have 2 increase If the first digit and second digit rotary switches are set to 3 and 5 respectively the compensation value is 53 To increase the HGB and WBC data decrease the compensation value In this case to have 2 increases at HGB and WBC data change the compensation value 53 to 43 because 2 0 2 10 Therefore set the second digit rotary switch to 4 Note that the RBC and PLT data have 4 2 increase when the HGB and WBC data have 2 increase SWO012 DIL ADJ 1 SWO11 DIL ADJ 2 Calibrating Touch Screen Pos A YY Calibrate the touch screen when the pressed position and operationg position do not match 1 Press the Capillary mode key while holding down the Reset key The TOUCH SCREEN CALIBRATION screen appears TOUCH SCREEN CALIBR
83. background noise increases Yes No l l l l l Service Manual MEK 7222 6 27 6 MAINTENANCE 6 28 SENSOR MONITOR screen Write each sensor output voltage on the SENSOR MONITOR screen as follows MANO UPPER ____ V under no fluid condition Acceptable range 3 5 V or more MANO LOWER ____ V under no fluid condition Acceptable range 3 5 V or more DILUENT ____ V under no fluid condition Acceptable range 3 5 V or more HEMOLYNAC 5 Vunder no fluid condition Acceptable range 3 5 V or more HEMOLYNAC 3 WVunder no fluid condition Acceptable range 3 5 V or more MANO UPPER V filled with fluid Acceptable range 1 5 V or less MANO LOWER V filled with fluid Acceptable range 1 5 V or less DILUENT V filled with fluid Acceptable range 1 5 V or less HEMOLYNAC 5 V filled with fluid Acceptable range 1 5 V or less HEMOLYNAC 3 V filled with fluid Acceptable range 1 5 V or less HGB LED ON V with fluid Acceptable range 1 5 V to 4 5 V HGB LED OFF V with no fluid Acceptable range 0 5 V or less ELECTRODE Vfilled with fluid Acceptable range 17 7 V to 18 3 V MJ Warmer temperature C filled with fluid CD Temperature inside chassis C filled with fluid DILUTER CF ____ Compensation of dilution ratio MEAS UNIT CF ___ Compensation of manometer volume CIRCUIT CHECK screen Write each value on the CIRCUIT CHECK screen as follows WBC Acceptable range 8 045 RBC
84. be to this joint insert the tube into the fitting nut and turn the fitting nut clockwise to fasten it ine O ring Tube a Tube stopper nu Board and Unit Location This following pictures show the location of the boards and units in the hematology analyzer Right Side Inlet outlet unit Front panel unit Power supply unit Sheath unit Left Side Laser optical unit Flow cell adjuster Printer unit Option AMP CONTROL board Service Manual MEK 7222 43 4 DISASSEMBLY AND ASSEMBLY Upper Part of Right Side Diluter unit Lower Part of Right Side Triple pump unit Front View of Chassis Sampler unit Rinse unit and switch assembly CBC measuring unit DRIVER board 4 4 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Draining the Hematology Analyzer Service Manual MEK 7222 To avoid any trouble drain all the fluid from the hematology analyzer Some of the units described in this section require the following procedure 1 N Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning Disconnect the diluent tube from the ISO3 inlet the hemolysing reagent tubes from the HEMO3 and HEMOS inlets and the detergent tubes from the CLN and CLN3 inlets on the right side panel CAUTION Do not disconnect the waste fluid tube from the WASTE outlet 3 Press the DRAIN ALL key on the OPERATIONS screen OPERATI ONS
85. ber of counts that filters and pump tubes are used to determine the maintenance schedule When filters or tubes are used more than the following number of sample counts the messages appears on the READY screen to prompt you to check and replace them Filters 1000 counts Pump tubes 3000 counts Cap pierce needle 1000 counts Displaying the 1 Press the OPERATION HIST key on the OTHER screen to display the OPERATION HISTORY OPERATION HISTORY screen Screen 14 FEB O3 15 22 Total number of counts Total operating hours TOTAL COUNTS 1972 counts A e id Number of counts DE HOURS 277 hours the filters are used EIE 8 1000 counts RESET Number of counts the E h pump tubes are used A 8 3000 counts RESET Number of counts the cap pierce NEEDLE 1 1000 counts CRESET LE Ee needle is used only when the cap pierce unit is installed o ax VERSION V02 01 The screen shows the total operating time total number of counts and number of counts that filters and pump tubes are used After checking and replacing filters or tubes and the cap pierce needle reset the counts to zero by pressing the RESET key 2 Press the OK key to return to the OTHER screen Service Manual MEK 7222 6 7 6 MAINTENANCE Checking Cleaning or Replacing Filters Check and clean the filters once a week or every 300 sample counts Replace them when they are clogged or dirty every 1000 sample counts 1 Press the STRONG CLEAN key on
86. bs of the sample cup and push the cup back to remove it Remove the HGB cover of the WBC measurement bath by removing the screw 10 Loosen the screws fastening the measurement baths The screws cannot be removed from the measurement baths 11 Remove the measurement baths and sub baths by pulling them together 12 Soak the measurement baths sub baths and sample cup in CLEANAC 3 detergent for about 10 minutes 13 Rinse the measurement baths sub baths and sample cup with water and wipe them with a dry cloth 14 Reattach the baths and cup to its original position and tighten screws Make sure that the baths are attached firmly especially the sub baths 15 Replace the HGB cover and fasten it with the screw 16 Reattach the front cover and fasten it with two screws on each side 17 Turn on the power The hematology analyzer starts priming the fluid path Service Manual MEK 7222 6 11 6 MAINTENANCE Checking and Cleaning the Rinse Unit and Sampling Nozzles Check and clean the rinse unit and sampling nozzles once a month or every 1000 sample counts 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths o O 4 3 Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws
87. caps soak the aperture caps in CLEANAC 3 detergent for about an hour CAUTION Handle the aperture caps with care It may be damaged if a sharp object is used to clean it 15 20 Rinse the aperture caps with water and reattach them to the original position There are separate aperture caps for WBC and RBC Be careful not to attach the aperture caps to the wrong positions Attach the WBC aperture cap white O ring to the white side Attach the RBC aperture cap red O ring to the red side Check that the red or white O ring is at the front Return each bath to the original position and tighten the screws Make sure that the baths are attached firmly especially the sub baths Fasten the HGB cover with the screw Reattach the front cover and fasten it with the two screws on each side Turn the power on The hematology analyzer starts priming the fluid path Count a diluent sample and check that the background noise is decreased Refer to Measuring Background Noise in Section 2 Service Manual MEK 7222 6 MAINTENANCE Checking and Replacing the Sampling Nozzles o O Y Service Manual MEK 7222 Check the sampling nozzles once a month or every 1000 sample counts taking the following steps When PLT background noise increases replace the sampling nozzles with a new one The plastic part of the sampling nozzle for the MEK 7222J K hematology analyzer is colored blac
88. coefficient obtained in step 2 using the numeric keypad The entered value appears in the VALUE box 5 Press the ENTER key to register the value When you register a new calibration coefficient the measured data also changes Change all necessary values 6 Press the OK key to return to the CALIBRATION screen Service Manual MEK 7222 Service Manual MEK 7222 8 DEMONSTRATION GUIDE Calibration by Measured Data 1 Press the VENOUS key on the CALIBRATION screen to display the CALIBRATION VENOUS screen 2 Select the desired parameter by pressing the parameter key As you press the key the cursor moves between the DATA and CAL fields Measured value Calibration coefficient Parameter key CALIBRATION VENOUS 14 Feea3 12 00 C 7 3 1000 Pis value BEC 4 26 JE 1000 VALUED SD 12 7 11 1000 aD E 33 6 11 1000 D 90 7 pen 29 9 Numeric keypad MCHC 33 0 GU C 225 JC 1000 OK key to return to the CALIBRATION screen NEXT key to go to Enter key to register another page the entered value 3 Move the cursor to the DATA field and enter the median of the assay value listed in the assay sheet using the numeric keypad The entered value appears in the VALUE box 4 Press the ENTER key to register the value When you register new measured data the calibration coefficient also changes Change all necessary values 5 Press the OK key to return to the CALIBRA
89. d VR042 LOW VR variable resistors so that each sensor output voltage is 3 5 V or more 11 Press the OPERATIONS key on the MENU screen The OPERATIONS screen appears 5 2 Service Manual MEK 7222 5 Adjustment 12 Press the PRIME key on the OPERATIONS screen The PRIMING message appears on the screen When the WBC and RBC measurement baths fill with diluent watch the manometer window and check that the manometer also fills with diluent Also check that the two LEDs D041 UPPER and D042 LOWER light NOTE Since the threshold for fluid detection in the manometer is set to around 2 5 V an alarm such as UPPER MANO ERROR or LOWER MANO ERROR can occur when the sensor output voltage comes closer to 2 5 V When the difference between the sensor output voltages with fluid and without fluid becomes less than 2 V a bubble alarm can frequently occur because the difference voltage is not enough to discriminate between the two statuses To maintain the difference voltage 2 V or more clean the manometer completely before checking the sensor output voltages Compensating Manometer Volume Service Manual MEK 7222 SWO12 DIL ADJ 1 SWO11 DIL ADJ 2 Label on MC unit side When the manometer is disassembled and assembled or replaced with a new one perform the compensation of the manometer volume Refer to the following example The label of the default compensation value set at our factory is attached to the CBC measuring u
90. d leakage on the hematology analyzer Yes No l Safety There is no damaged AC plug and exposed wire on the power cord Yes No l A f i 3 pin plug type power cord is used and the 3 pins and plug housing are not deformed Yes No Resistance of the protective ground line of the power cord is 0 1 U or less Yes No Earth leakage current is 0 5 mA or less under normal condition Yes mA No Earth leakage current is 1 0 mA or less under each single fault condition Yes mA No l Reagents Nihon Kohden recommended diluent detergent and hemolysing reagent are used Yes No ISOTONAC 3 diluent is not past the expiration date Yes No CLEANAC detergent is not past the expiration date Yes No CLEANAC 3 detergent is not past the expiration date Yes No HEMOLYNAC 3 or HEMOLYNAC 3N hemolysing reagent is not past the expiration date Yes No HEMOLYNAC 5 hemolysing reagent is not past the expiration date Yes No l Cleaning Replacing Rinse unit is cleaned Yes No Cap pierce needle is cleaned Yes No l Two filters are replaced with new ones Yes No l l Two pump tubes are replaced with new ones Yes No i Sampling cup Bath for WBC 5 part differential measurement is cleaned Yes No Two sub baths and two measurement baths are cleaned Yes No Two aperture caps are cleaned Yes No Touch screen is cleaned with a soft cloth moistened with 80 alcohol Yes No Two sampling nozzles are cleaned or replaced with new ones if they are damaged or PLT
91. djust the flow cell unit until the CV of FS and FL is minimum and the peak is maximum NOTE Check the CV and PEAK values by measuring the polymer microsphere suspensions again after adjusting the flow cell unit If the CV values obtained from measuring new polymer microsphere suspensions which are not coagulated are not optimum after adjusting the flow cell unit the hematology analyzer may be damaged Contact your Nihon Kohden distributor 8 14 Service Manual MEK 7222 8 DEMONSTRATION GUIDE Adjusting Gain FS and FL NOTE for WBC 5 Part Differential Adjust the FS and FL gain only when the PEAK values are not optimum Parameters in Rough Mode in the particle test Using Polymer Microsphere Suspensions 1 Display the SENS amp THRESHOLD screen for WBC 5 part differential ES Refer to the Cleaning the Fluid Path in the Hematology Analyzer parameters by doing one of the following a Press the GAIN key on the PARTICLE TEST screen b Press the SENS amp THRESHOLD key on the SETTINGS screen and press the DIFF key NOW Current peak Press this key to select sample type from AIM Target peak PARTICLE BLOOD and CONTROL In PARTICLE mode the 12 FEB 03 scattergram of the polymer l microsphere suspensions is displayed The entered value ED 62 GDL 60 299 Et 301 ya n O aD Press OK key to return we GE s the entered value to the SETTINGS screen The appropriate setting gain In PARTICLE
92. e the provided power cord Using other power cords may result in electrical shock or other injury to the operator Equipotential Grounding When equipotential grounding is required connect the equipotential ground terminal on the rear panel of the hematology analyzer terminal marked by Y to the equipotential ground terminal on the wall equipotential grounding system with the equipotential grounding lead potential equalization conductor WARNING For operator safety equipotential grounding of all instruments must be performed Consult with a qualified biomedical engineer Insert the laser key into the laser switch hole on the right side panel and turn the laser switch to the side marked by HE NOTE When the measurement is performed with the laser switch turned off CBC can be measured but there is no WBC 5 part differential scattergram and data and an alarm occurs Store the key in a safe place Service Manual MEK 7222 Turning On the Power Main power lamp Power lamp Laser lamp Power key Service Manual MEK 7222 8 DEMONSTRATION GUIDE 1 Press the main power switch on the rear panel of the hematology analyzer to on The main power lamp on the front panel lights 2 Press the power key on the front panel to on The power lamp lights When the laser switch on the right side panel is turned on the laser lamp also lights Checking the reage
93. ean the measurement bath Refer to Section HGB value 9 Incorrect LCD display Circuit error Press the Reset key If the problem still F Instrument repeats same occurs turn off the instrument wait about 10 operation seconds then turn it on again Paper jammed Remove the jammed paper Refer to the printer manual G No printing Circuit error Press the Reset key If the problem still Occurs turn off the instrument wait about 10 seconds then turn it on again The pressed position and operating Calibrate the touch screen Refer to Section 9 position do not match The touch screen keys do Em Circuit error Press the Reset key If the problem still H not function occurs turn off the instrument wait about 10 seconds then turn it on again 2 6 Service Manual MEK 7222 Problem Priming starts suddenly the instrument program and the READY screen appears screen is not correct the alloted area K Flags related to WBC 5 part differential frequently appear Service Manual MEK 7222 When noise interferes with Possible Cause Criteria Power cord is not connected properly 2 TROUBLESHOOTING Countermeasure Connect the power cord properly priming automatically starts Equipment near the instrument is generating noise Separate the instrument from other equipment and their power sources Noise in the power line The date and time setting is not correct Use a different
94. ear rubber gloves to protect yourself from infection when handling and measuring blood samples 1 Count more than 10 human blood samples from different healthy people which are 30 minutes to 8 hours after collection 2 Check that none of the following flags is displayed Press the flag key to check this Immature Granulocyte Blasts TOCSY 12 FEB OS 16 32 panan Hey 8 do 4 Left Shift F i Atypical Lymphocyte d E Eosinophilia e i Flag Display Screen b The Flag key appears and flashes on the screen if there is flag Press the flag key to display the flag display screen There is no flag if the flag key is not displayed Flag key O 12 Fee Os 16 32 Flaca NE 2 6 S Measured data Press the flag display Blasts 3 3 J Press here to display the area to return to the Cerai 927 magnification of the data previous screen If there is a flag repeat the procedure in the Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous Blood Samples section Perform the calibration again if the data obtained in the above measurement differs from the data obtained using other instruments or at other test facilities Calculate the new calibration coefficient for each parameter from the mean value of measured data obtained by the MEK 7222J K hematology analyzer and other means 8 28 Service Manual MEK 7222 8 DEMONSTRATION GUIDE Interference Substances WBC HGB HCT Unlysed red cells In some
95. eeeeseneeeteaes 5 5 Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions coocccoconcccccononcnnnonanannnnnnonannncnnonn cnn nnnnn na nannncinns 5 9 Adjusting Gain for WBC 5 Part Differential Measurement Finely by Human Blood Sample coo ii E ble ide ete ea agone dus 5 10 Editing the Peak EISE eroi teta tein xe ous ner aine teles ele 5 13 Manually Adjusting the Gain sessssessesssesseseeeeneenneee enne nnne 5 15 Compensating Dilution Ratio ooooconnncinnnnnncnnnncccnnnccccnnnannnn narran anna rca carr 5 15 Calibrating TOUCH Screens EU 5 16 Adjusting Liquid Sensor Output Voltages ooonoocccnnncccccnoccncononcnonannanano narran cnn ran rca rancia aSa 5 17 Adjusting Contrast of LCD ococcinnccccnnoccccononnnnonnnnnnnnnn co nonn cnn cnn n conan rca narran rare rara rra 5 18 Section 6 Maintenance a 6C 1 To be Replaced Periodically aia ads 6 1 Periodic Maintenance Check Procedure cooooocccnocccccnoccccconccnnnancnnnonnnnnnannn nara ncn enne nnne nnns 6 1 i leida 6 1 files 6 1 Siu eeina 6 1 FROAGOINS E 6 1 Gleaning REplaciO O msn ts 6 2 SENSOR MONITOR screen concccccnocccconccnnonnnncnnnnnnnnnnnnnnnone nn rca nennen nennen nennen nennen 6 3 CIRCUIT CHECK Sereehi iioi in 6 3 Background MOISE irreal 6 4 Measuring the Polymer
96. el 1 TP073 GATE Test point for output waveform from the gate circuit for 5 part differential WBC TPO74 RBP Test point for output waveform of Robinson gate pulse for 5 part differential WBC TPO75 RB1 Test point for output waveform from the Robinson gate circuit for 5 part differential WBC TPO76 RBTHR Test point for threshold level on the Robinson gate circuit for 5 part differential WBC TP077 PHA1 Test point for output waveform from the peak hold circuit for FS channel 1 TPO78 CL1 Test point for output waveform from the baseline stabilizer circuit for FS channel 1 TPO79 PEAK HOLD Test point for the peak hold control signal for 5 part differential WBC TP081 ANA2 Test point for output waveform for FL channel 2 from MO 820V laser optical unit TPOS2 FIL2 Test point for output waveform through the low pass filter for FL channel 2 TP083 CL2 Test point for output waveform from the baseline stabilizer circuit for FL channel 2 TP084 PHA2 Test point for output waveform from the peak hold circuit for FL channel 2 TP091 ANA3 Test point for output waveform for SD channel 3 from MO 820V laser optical unit TPO92 FIL3 Test point for output waveform through the low pass filter for SD channel 3 7 18 7 Test Point Variable Resistor LED and Switch on Board DRIVER board 7 14 TP093 CL3 Test point for output waveform from the baseline stabilizer circuit for SD channel 3 TP094 PHA
97. ematology analyzer starts priming the fluid path 14 Display the OPERATION HISTORY screen and press the RESET key for TUBES to reset the counts to zero Service Manual MEK 7222 6 15 6 MAINTENANCE Cleaning the Aperture Caps 6 16 For daily cleaning of the aperture caps press the Clean key on the front panel However if the clogged messages frequently appears or background noise is still high clean the aperture caps taking the following steps 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Remove the diluent tube from the ISO3 inlet the hemolysing reagent tubes from the HEMO3 and HEMOS inlets and the detergent tubes from the CLN and CLNG inlets on the right side panel Do not disconnect the waste fluid tube from the WASTE outlet 3 Press the DRAIN ALL key on the OPERATIONS screen A confirmation message appears on the screen 4 Press YES to start draining the hematology analyzer 5 After draining press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 6 Remove the two screws on each side of the front cover of the hematology analyzer 7 Open the front cover by pulling it from the right side Service Manual MEK 7222 6 MAINTENANCE Sub baths 8 Remove the HGB cover of the WBC measurement T Ss bath by removing the screw
98. en and press the TOTAL HOURS 277 hours RESET key for NEEDLE to reset the counts to zero ERRATA IA ee LIS 8 1000 counts GUN ES 38 3000 cants CM NEEDLE 1 1000 counts oK VERSION 02 01 51F2 gt 6 24 Service Manual MEK 7222 6 MAINTENANCE Calibrating Touch Screen Calibrate the touch screen when the pressed position and operating position do not match xA 1 Press the Capillary mode key while holding down the Reset key The TOUCH SCREEN CALIBRATION screen appears TOUCH SCREEN CALIBRATION Press the exact center of the mark in the lower right corner 2 Follow the instructions on the screen to calibrate the screen TOUCH SCREEN CALIBRATION e mark NOTE Do not use a sharp object to press the mark Use your finger After calibration is completed the screen returns to the READY screen Service Manual MEK 7222 6 25 6 MAINTENANCE This Page is intentionally left blank 6 26 Service Manual MEK 7222 aley mo 6 MAINTENANCE Maintenance Check Sheet Fill out and save this check sheet each time you do maintenance or service Date Customer Customer Address Service Personnel Service Company Instrument Name Hematology Analyzer Instrument Model MEK 7222J K Instrument Serial Number Hardware Revision l Software Version l l Appearance l l There is no damage on the hematology analyzer Yes No i There is no flui
99. en the CV value from the particle test is over the normal range it is probably caused by dust or bubbles inside the flow cell Check the following items before adjusting the flow cell unit position 1 Did you clean the fluid path and flow cell in the hematology analyzer by pressing the Clean key on the front panel or by pressing the CLEAN FLOWCELL key on the OPERATIONS screen You need to completely remove air bubbles from inside the flow cell with the CLEANAC detergent when the hematology analyzer is first installed Bubbles inside the flow cell cannot be removed by priming at power on or strong cleaning Bubbles and dust can be removed by cleaning the flow cell by pressing the Clean key on the front panel or pressing the CLEAN FLOWCELL key on the OPERATIONS screen If the CV from the particle test is over the normal range perform cleaning by pressing the Clean key on the front panel or CLEAN FLOWCELL key on the OPERATIONS screen 2 CLEANAC and CLEANAC 3 detergents are connected correctly If CLEANAC and CLEANAC 3 detergents are connected incorrectly bubbles inside the flow cell can not be removed Connect CLEANAC and CLEANAC 3 correctly and clean the hematology analyzer by pressing the Clean key on the front panel or CLEAN FLOWCELL key on the OPERATIONS screen Service Manual MEK 7222 MANUAL ADJUSTMENT 12 FEB 03 14 04 FS FL 60 x COUNT 6595 FS FL POSITION CY 5 0 4 5 NOW START 2 PEAK 58 92 4937 5000 O
100. ening the Front Cover of Section 4 Disassembly and Assembly 6 Perform the following checking cleaning and replacing Cleaning the rinse unit Clean the rinse unit according to the procedure described in Checking and Cleaning the Rinse Unit and Sampling Nozzles in this section Cleaning the cap pierce needle Clean the cap pierce needle according to the procedure described in Checking and Cleaning the Cap Pierce Rinse Unit Sampling Nozzle and Cap Pierce Needle in this section Replacing the filters Replace the filters with new ones according to the procedure described in Checking Cleaning or Replacing the Filters in this section Replacing the pump tubes Replace the pump tubes with new ones according to the procedure described in Checking and Replacing the Pump Tubes in this section Cleaning the sub baths measurement baths and sampling cup Clean the sub baths measurement baths and sampling cup according to the procedure described in Checking and Cleaning the Sub Baths Measurement Baths and Sampling Cup in this section Cleaning the aperture caps Clean the aperture caps according to the procedure described in Cleaning the Aperture Caps in this section Cleaning the touch screen Clean the touch screen with a soft cloth moistened with 80 alcohol Service Manual MEK 7222 SENSOR MONITOR Screen CIRCUIT CHECK Screen Service Manual MEK 7222 6 MAINTENANCE Checking and rep
101. ent Otherwise the hemoglobin concentration decreases Service Manual MEK 7222 8 DEMONSTRATION GUIDE e When using HEMOLYNACe3N reagent attach the HEMOSN label provided with the hematology analyzer to cover the HEMOS label at the HEMOS inlet Refer to Connecting tubes 2 in section 2 of the MEK 7222 operator s manual Inlet Outlet on the Reagent Container Cap Tube Tube ASSY hematology analyzer 18 L diluent tube assy ISOTONAC 18 L cal S 1 Diluent ISOTONAC 3 j oe A Cleanac tube assy 8222 p 2 Detergent CLEANAC q N Ea YT SZ ee Cleanac tube 8 Detergent CLN3 3 CLEANAC 3 Inlet C Hemolysing reagent Hemolynac3 tube assy am 4 HEMOLYNAC 3 or Hemolynac3 C x edo HEMOLYNAC 3N cap Hemolynac5 tube assy 5 Hemolysing reagent HEMOS HEMOLYNAC 5 NU 3A Inlet E 18 L tube assy 8 Attached to WASTE pida WASTE Se Waste tltno Container the container a Outlet F Service Manual MEK 7222 8 5 8 DEMONSTRATION GUIDE Connecting the Power Cord and Grounding the Hematology Analyzer Tov A Equipotential ground terminal AC SOURCE socket Turning the Laser Switch 8 6 Connecting the Power Cord Connect the provided power cord to the AC SOURCE socket on the rear panel and plug the cord into a 3 prong AC outlet on the wall CAUTION Only us
102. ere suspensions Then adjust the gain minutely by measuring human blood Observe the instructions on the 7 Lm polymer microsphere suspensions manual to achieve optimum performance and safety Service Manual MEK 7222 5 Adjustment CAUTION Do not swallow the polymer microsphere suspensions If swallowed contact your physician immediately e Avoid contact with the mouth and eyes If the polymer microsphere suspensions contacts the mouth or eyes wash thoroughly and immediately with water and contact your physician immediately Counting the Polymer Microsphere Suspensions and Adjusting the Flow Cell Position The hematology analyzer uses the light scatter technique to differentiate WBC into 5 parts To ensure accurate laser beam scatter for the sample blood cells count the polymer microsphere suspensions and adjust the flow cell position 1 Clean the flow cell to remove dusts or bubbles inside the flow cell Press the CLEAN FLOWCELL key on the OPERATIONS screen to clean the flow cell Refer to Cleaning the Flow Cell in Section 9 14 FEB 3 15 21 NOTE After cleaning if an alarm appears to indicate there is not sufficient amount of diluent detergent or hemolysing reagent in the bottle refill the bottle and clean the flow cell again 2 Prepare about 1 mL of polymer microsphere suspensions in a sample container When the MS 721V cap pierce unit is installed select OPEN mode on the ORDER screen
103. ergent and hemolysing reagent are used Check that these reagents are used before the expiration date Especially the expiration date of the Hemolynac 3 hemolysing reagent which includes cyanogen is 30 days after the package is opened while the expiration date of the Hemolynac 3N non cyanogen type is 90 days after the package is opened 6 1 6 MAINTENANCE Cleaning Replacing 6 2 If you use Hemolynac 3 which is past the expiration date the HGB value can be lower than the actual value because the cyanogen is deteriorated If Hemolynac 3 which is past the expiration date is used and the HGB value of the hematology control is lower than the assay sheet suggest the customer to replace the Hemolynac 3 with a new one If a facility does not have many blood samples and a regular size bottle of hemolysing reagent is not consumed by one hematology analyzer within the expiration date suggest the customer to divide the hemolysing reagent into several smaller bottles for several hematology analyzers 1 Press the STRONG CLEAN key on the OPERATIONS screen to start strong cleaning 2 Disconnect the diluent detergent and reagent tubes other than the waste fluid tube from the right side panel of the hematology analyzer 3 Press the DRAIN ALL key on the OPERATIONS screen to drain all the fluid inside the hematology analyzer Turn off the main power switch on the rear panel Open the front cover according to the procedure described in Op
104. etas rece an 4 13 Removing the Sampler Unit ioc dense iio 4 15 Removing the Pump UNit ooooocococco nananccononnnanancnnnnnnnnnnnonnn corran rre 4 16 Removing the Triple Pump Unit a cs 4 18 Removing the WA 720VK Printer Unit Option oooconccccnnnccccnnocccnnonacanonanccnnnaacannanaca canario 4 19 Removing the Front Panel Unit oooooonccocccnncnoncconnnonananccnnnonnnnnnno nono n nana EE 4 20 Removing the Inlet Outlet Unit ooooococonncccninncccnnnccccnnnccnnnnrn co nanrn nara cnn nn rca 4 22 Removing the Flow Cell AdjusteF ooooconccccnnnncccinocccnoncccnnonnnccnonnn non nano cacon eene nnn 4 24 Removing the Laser Optical Unit eene nennen nnne nnns 4 26 Removing the SheathiUnit sssaaa pee nens aat rt etas Dea pasce ERE euadere eR Eamus 4 28 Removing the Rinse Unit and Switch Assembly sssssseeeen 4 31 Replacing the AMP CONTROL Board coocccconocccconoccccnonocanonnncnnnnnoncnconnnannnnnn cc nano cnn narra nennen 4 32 Replacing the DRIVER Board teinte reet eceteee stet teer tree ecd c aed neun at 4 33 Replacing the VOLUME Board KEY DISPLAY Board and LCD Unit usss 4 34 Replacing the MS 721V Cap Pierce Unit Option sssssee 4 36 Service Manual MEK 7222 4C 1 Before You Begin Warnings and Cautions Required Tools Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY The procedures in this section tell how to remove replace and install major
105. etermined from HGB and RBC values Therefore the limitations for HGB and RBC also affect MCH value MCHC is determined from HGB and HCT values Therefore the limitations for HGB and HCT also affect MCHC value RDW is determined from RBC value Therefore the limitations for RBC also affect RDW value Agglutinated RBC Agglutinated RBC may decrease RBC count and erroneous RDW This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Nutritional deficiency or blood transfusion Iron and or cobalamin and or folate deficiency may increase RDW Very small fragments Very small RBC RBC fragments and WBC fragments may be the cause in increased PLT count Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in decrease in PLT This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Very large PLT Large PLT may exceed the PLT threshold and might not be counted which results in low PLT count Chemotherapy Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count In such a case measure manually Hemolysis Hemolyzed specimens contain red cell stroma which may increase PLT count Anticoagulated blood Blood anticoagulated with acid citrate dextrose may have clumped PLT which may cause decrease in PLT count Agglutinated PLT Clumped PLT may decrease PLT count and or increase WBC count For such sample collect the sample in
106. ey after finishing registration The ADJUST DIFF GAINS message appears AUTO DIFF GAIN 12 FEB O3 15 41 REED BLOOD ID 0000000001230 DATE 12 FEB 3 TIME 15 48 MO PEAK N D NOW AVE Al GAIN FS 72 gt 62 FL 62 9 61 A 20 30 7 Press the YES key to change diff gains and the peak list of the registered blood sample is initialized AUTO DIFF GAIN 12 FEB O3 15 41 000 000 BLOOD NO DATA FOUND MO PEAK CN AVE AIN X 9 9 m us am Check the gain with a human blood sample after changing the gain Service Manual MEK 7222 5 Adjustment Editing the Peak List 1 Press the LIST key on the AUTO DIFF GAIN screen to check and delete a registered blood sample on the PEAK LIST screen ID keys of the registered samples The number of the registered samples The date and time of measurement ii F N 12 registered samples MO istribution ES 187 9 7 0 Peaks of MO distribution E S587 350 The average of WBC peak of SD 25 0 5 0 the registered sample LY Peaks of LY distribution Ep 36 07 8 0 SD 20 0 0 0 NE istribution ii 55 8 12 8 Peaks of NE distribution EE 58 0 OUER MED Delete registered sample from the peak list Go to another page Print the list book 2 Press the sample ID key to return to the AUTO DIFF GAIN screen where the scattergram of the chosen blood sample can be checked Check Points The MO and LY scattergrams appear with
107. he Reset key Clean key and Dispense Open key 12 While pressing the Reset key press the Capillary blood mode key on the front panel The TOUCH SCREEN CALIBRATION screen appears Adjust the touch screen properly Refer to Calibrating Touch Screen in Section 9 Maintenance of the operator s manual 13 Initialize the settings according to Initializing Settings in Section 3 Changing Settings of the operator s manual 14 Enter the settings which you wrote down in step 1 Replacing the DRIVER Board ha Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Remove the top cover according to the procedure described in Removing the Top Cover 3 Remove the rear cover according to the procedure described in Removing the Rear Cover Including the Power Supply Unit Disconnect the 8 connection cables from the DRIVER board Remove the 4 screws which secure the DRIVER board to the chassis Remove the DRIVER board from the chassis a2 UT X P JE us os a m m 435 c ree Service Manual MEK 7222 4 33 4 DISASSEMBLY AND ASSEMBLY 6 Attach the new DRIVER board to the chassis with the 4 screws 7 Reverse the above procedure to assemble the hematology analyzer Replacing the VOLUME Boa
108. he assignment of values to fresh blood used for calibrating automated blood cell counters ICSH The assignment of values to fresh blood used for calibrating automated blood cell counters 1 3 1 GENERAL HGB NCCLS H15 A2 H15 A2 Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in Blood Second Edition Approved Standard 1994 HCT NCCLS H7 A2 H7 A2 Procedure for Determining Packed Cell Volume by the Microhematocrit Method Second Edition Approved Standard 1993 PLT Brecher amp Cronkite Interference Substances WBC Unlysed red cells In some rare occasions the RBC in the blood sample may not completely lyse and these non lysed RBC may be detected as WBC and cause increase in WBC count Multiple myeloma The precipitation of proteins in multiple myeloma patients may increase the WBC count Leukemia WBC is fragile in leukemia patient and WBC may be destroyed during measurement These WBC fragments may also interfere with WBC differential measurement Chemotherapy Cytotoxic and immunosuppressive drugs cause low WBC count Cryoglobulins Cryoglobulin may be increased in patients who are or have myeloma cancer leukemia macroglobulinemia lymphoproliferative disorders metastatic tumors autoimmune disorders infections anerurism pregnant thromboembolic phenomena diabetes etc which cause increase in WBC RBC or PLT counts and HGB concentration In such cases warm the blood sample to 37 C in a water
109. ient Equipment near the instrument is Separate the instrument from other equipment Noise interference during P A generating noise and their power sources counting Noise in the power line Use a different power line The front cover is open Close the front cover Dirty diluent Replace the diluent Dirty sub bath measurement bath and Clean the sub bath measurement bath and filters filters Refer to Section 9 Dirty aperture caps Clean the aperture caps Refer to Section 9 Poor contact of external electrode to the B High background noise Firmly tighten the measurement bath socket of the instrument Press the Clean key to clean the fluid path i using the CLEANAC detergent Perform the The fluid path and diluent tube are dirty i strong cleaning by selecting STRONG CLEAN on the OPERATION screen e Stir the sample sufficiently without creating Insufficient stirring of sample bubbles Clean the sub bath and or measurement bath Poor reproducibility of Dirty sub bath and or measurement bath C Refer to Section 9 blood cell count Dirty aperture caps Clean the aperture caps Refer to Section 9 i Reduce the background noise Refer to High background noise Problem B Water leaks from inside Pump tube is broken Replace the pump tube Refer to Section 9 D the hematology analyzer Filter is clogged Replace the filter Refer to Section 9 E Poor reproducibility of The WBC measurement bath is dirty Cl
110. ients as follows New Calibration Coefficients WBC RBC HGB HCT PET NE Venous blood mode LY Wenous blood mode MO Wenous blood mode EO Venous blood mode BA _____ Venous blood mode RDW Venous blood mode MPV ____ Venous blood mode 6 MAINTENANCE Write down the lot number of the MEK 3DN hematology control new calibration coefficients and measured data as follows Lot No of MEK 3DN Venous blood Capillary blood Parameter Measured data Calibration coefficient Measured data Calibration coefficient WBC RBC HGB HCT PLT NE LY MO EO BA RDW MPV Service Manual MEK 7222 6 29 6 MAINTENANCE 6 30 Software Version Write down the software version on the OPERATION HISTORY screen Software version Operations Touch screen function is checked Yes No Date and time settings are checked Yes No Automatic cleaning operations are normal during this maintenance check Yes No There is no fluid leakage on the hematology analyzer Yes No Constant volume of the diluent is properly dispensed when pressing the Dispense Open key on the front panel in capillary blood mode Yes No Cap pierce unit properly works when the cap pierce unit is installed and the Open mode or Closed mode is selected Yes No Built in Printer Unit Operation When the printe
111. ights when automatic printing mode is selected optional 9 Auto print key Switches the printing mode between automatic and manual for the WA 720VK printer unit optional 10 Feed key Feeds paper of the WA 720VK printer unit while held down optional 11 Print key Prints measured data on the WA 720VK printer unit optional Printer unit 12 WA 720VK Roll printer Prints out measured data and sample ID no optional 13 Printer door For the recording paper of the WA 720VK printer unit To open pull the upper right corner optional 14 Betten brightness Adjusts the screen brightness control 15 Capillary blood Lights when in capillary blood mode mode lam Capillary blood 16 mode key Switches the operation mode to capillary blood mode The capillary blood mode lamp lights 17 Dispense key Dispenses diluent approx 2 mL from the sampling nozzle Available only for the capillary blood mode Operation indicator Blinking Aspirating the sample 18 os Off Counting the sample ps Lit Ready for next counting 19 Sampling nozzle Aspirates the sample Dispenses the diluent when in the capillary blood mode 20 Count switch Aspirates the sample and starts counting 1 8 Service Manual MEK 7222 Right Side Panel A Refer to Connecting the Tubes in Section 2 of the operators manual 1 GENERAL o 1
112. in the allotted area ABCDEF in the MAIN scattergram The NE scattergrams appear within the allotted area FEGHIJ The MO scattergrams appear within the allotted area KLOP in the MO BA scattergram The LY scattergrams appear within the allotted area LMNO in the MO BA scattergram The NE scattergrams appear within the allotted area QRST in the NE EO scattergram Flags do not frequently appear MO scattergram NE scattergram MO scattergram NE scattergram P F J KP Q T LY scattergram L O Ec R S LY scattergram M N MAIN MO BA NE EO Service Manual MEK 7222 5 13 5 Adjustment 3 To delete undesirable data from the list 1 Press the DELETE key on the PEAK LIST screen The SELECT FIRST DATA message appears M om m muc GGG mm coc oom Ud AT CA mine qub SOS OOO OOG NNN WM OSS I Ore com COO N Doo EDOD EEE ii Press the ID key of the first sample to be deleted The SELECT LAST DATA message appears ME ME Z Mo FS 187 0 7 8 EE 53 0 7 3 0 SD 25 0 5 0 EN FS 36 0 6 0 EE 38 0 8 0 SD 20 0 0 0 NE ES 160 0 12 0 Eb 120 0 16 0 78 4 OVER epee xo 111 The confirming message for deletion appears after pressing the ID key of the last sample to be deleted 12 FEB 12 FEB 12 FEB PEAK 15 480 16 21 16 26 Al E 187 187 0 7 8 53 53 0 3 0 25 25 0 5 0 36 36 0 6 0 38 38 0 8 0 20 20 0 0 0 168
113. int Variable Resistor LED and Switch on Board D0112 15V LED for check of 15 V DC for analog circuit When the 15 V DC is supplied to the analog circuit this LED is lit D0113 A12V LED for check of 12 V DC for analog circuit When the voltage is supplied to the analog circuit this LED is lit D0114 36V LED for check of 36 V DC for analog circuit When the voltage is supplied to the analog circuit this LED is lit SW021 POEWR Power switch SW022 Bit switches for power supply mode selection See the following table Bit switch Default Operation when the bit switch is set to ON No 1 OFF When the main power switch is turned on only 5 V DC is live No 2 OFF When the main power switch is turned on the 36 V 15 V 12 V A12 V 27 V Vu and Vp supply voltages are live Service Manual MEK 7222 7 11 7 Test Point Variable Resistor LED and Switch on Board AMP CONTROL board TPO112 TPO115 TP0114 TPO113 TPO111 TP013 TP014 TPO15 TPO16 TPO110 TPO11 TPO12 TPO19 TP113 TP114 TP111 TP112 TP076 TPO75 TP074 TP071 TPO81 TPO91 TPO61 TP072 TP082 TP092 TPO077 TPO78 TP084 TP083 TPO79 TP103 TP102 TP101 TPO11 5V 5 V DC terminal for digital circuit TPO12 ED Ground terminal for digital circuit TP013 12V 12 V DC terminal for valve control TP014 E12 Ground terminal for valve control TP015 A12V 12 V DC terminal for analog circuit 7 12 Service M
114. ion Description AC power off s03 ISOTONACe3 Disconnection from the mains diluent AC power on CLEANAC Connection to the mains detergent CLEANACe3 Main power lamp CLN3 detergent HEMOLYNACe3 On only for part of the equipment hemolysing reagent Off only for part of the equipment HEMOS HEMOLYNACe3N hemolysing reagent ECO OC 10 5 2 lt 4 gt T E 3 o o z a Z oo Screen brightness Reset WASTE WASTE ARS Clean Attention consult operator s manual Pd Capillary blood mode Alternating current lt gt Count Equipotential terminal py Dispense E Printer socket Auto print IOIOI El Serial port 1 O qe Coal El Print A Biohazard W Inlet Year of manufacture ke Outlet Serial number Q When using the HEMOLYNAC 3N hemolysing reagent attach the HEMO3N label over the HEMO3 label Service Manual MEK 7222 On screen and recorded data Symbol Description Symbol Description gt Beside WBC or RBC measured value Sample error Beside HGB measured value Dirty measurement baths Beside WBC measured value Poor hemolyzation Beside HGB measured value HGB voltage adjustment error Beside MCHC measured value Abnormal MCHC Beside HGB measured value HGB circuit error Beside WBC or PLT measured value Platelet coagulation Service Manual MEK 7222 Section 1 General slirere Ufouolnieee eL 1 1 SEN
115. istor for adjustment of output voltage from sensor for HEMOLYNAC3 reagent VRO14 LIQ3 Variable resistor for adjustment of output voltage from sensor in the sheath unit 7 4 Service Manual MEK 7222 SHEATH board 7 Test Point Variable Resistor LED and Switch on Board D022 D023 VR012 TP012 TP013 TPO14 TPO11 L N3GHOM NOHIN 74 o in Re o 1 N N v gt gt gt A ul gt A mo o TP022 TP021 TP023 691L 1N SW021 TPO11 EA Ground terminal for analog circuit TPO12 PRESSURE Test point for output voltage from pressure sensor TPO13 15V Test point for 15 V DC for analog circuit TPO14 15V Test point for 15 V DC for analog circuit TP021 TEMP_MIX Test point for temperature inside the mix chamber TPO22 HEAT STATUS Test point for heater operation TP023 TEMP_BOTTOM Test point for environmental temperature at the bottom of the instrument VRO11 GAIN Variable resistor for gain adjustment of pressure sensor VRO12 OFF SET Variable resistor for offset adjustment of pressure sensor D021 HEATER LED for operation check of the heater When the heater works this LED lights D022 BOTTOM HOT LED for operation check in high environmental temperature When the temperature control works the LED lights SW021 Switch for temperature setting See the following table Bit switch setting Combination OFF Bit 1 ON OFF Bit 2 Default Whe
116. ith water and dry thoroughly with a dry cloth 13 Reattach the O ring to the rinse unit and return the rinse unit and rinse unit cap lt gt 2 ICs to the original position 14 Reattach the front cover and fasten it with the two screws on each side 15 Turn the power on The hematology analyzer starts priming the fluid path Service Manual MEK 7222 6 13 6 MAINTENANCE Checking and Replacing the Pump Tubes Check the pump tubes for water droplets and leaks every day Replace the pump tubes when there are water droplets or leaks Once every 4 months or every 3000 sample counts NOTE Do not leave the pump tubes with water droplets or leaks on them This may erode them 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths o OQO A 3 Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 Check the pump tubes for water droplets and leaks If any droplet or leak is found replace the tube with a new one taking the following steps
117. k to avoid misuse Do not use the sampling nozzle which has a gray plastic part 1 Press the STRONG CLEAN key on the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths 3 Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 19 6 MAINTENANCE Sampling nozzle screws Tube holder Spacers Sampling nozzle Joint Sampling nozzle guide 6 20 Check the sampling nozzles for blood stains flaking of the coating or bending There are two sampling nozzles If the sampling nozzle needs to be replaced take the following steps Remove the screw tube holder and spacer from the sampling nozzle NOTE Be careful not to drop the screws tube holder and spacers into the hematology analyzer 8 Remove the joint from the sampling nozzle 9 Turn the sampling nozzle 90 counterclockwise and pull it up to remove it Insert the new sampling nozzle into the sampling nozzle guide attach the joint and fasten the nozzle with the screw Reattach the front cover and fasten it with the two screws on each side
118. lacing the sampling nozzles Check the sampling nozzles and replace them with new ones if they are damaged or PLT background noise increases according to the procedure described in Checking and Replacing the Sampling Nozzles in this section 10 Turn on the main power switch on the rear panel While pressing the Reset key on the front panel press the Power key on the front panel The power turns on without priming The READY screen appears Press the MENU key on the READY screen The MENU screen appears Press the OTHER key on the MENU screen The OTHER screen appears Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen which displays each sensor output voltage appears Check that the sensor output voltages of MANO UPPER MANO LOWER DILUENT HEMOLYNAC 5 and HEMOLYNAC 3 are 3 5 V or more under no fluid condition Write down each voltage on the check sheet Connect the diluent detergent and reagent tubes to the right side panel of the hematology analyzer Press the OPERATIONS key on the MENU screen The OPERATIONS screen appears Press the PRIME key on the OPERATIONS screen The screen returns to the READY screen after priming operation is completed Check that the following sensor output voltages are displayed on the SENSOR MONITOR screen Write down each voltage on the check sheet If there is a sensor output voltage out of the acceptable range adjust the sensor output voltage for the acceptable range
119. less x 10 AL TOC 100 or less 1 Measure the polymer microsphere suspensions according to the procedure described in Counting the Polymer Microsphere Suspensions and Adjusting the Flow Cell Position in Section 3 Changing Settings of the operator s manual 2 Check that the CV of FS and FL are 7 or less on the PARTICLE TEST screen If the CV is more than 7 clean the flow cell measure the polymer microsphere suspensions again and adjust the flow cell position 1 Write down the lot number of the MEK 5DN hematology control on the check sheet 2 Check the reproducibility by measuring the MEK 5DN hematology control Refer to Counting the MEK 5D Hematology Control in Section 6 Quality Control of the operator s manual 3 Check that the obtained results are within the acceptable range If the obtained data is out of the range go to New Calibration Coefficients 1 Press the CALIBRATION key on the MENU screen The CALIBRATION screen appears 2 Press the VENOUS key or CAPILLARY key on the CALIBRATION screen according to the facility s requirement The current calibration coefficients are displayed on the screen 3 Write down the calibration coefficients on the check sheet if the obtained results are within the acceptable range at Measuring the MEK 5DN Hematology Control in this section 1 Write down the lot number of the MEK 3DN hematology control on the check sheet 2 Measure the MEK 3
120. lled Needs measuring unit replacement Contact your Nihon Kohden representative Check the hematology analyzer by counting the hematology control Then recount the sample Replace the filters and reset the operation history Replace the pump tubes and reset the operation history Check or replace the cap pierce needle and reset the operation history 24 Service Manual MEK 7222 System Error Messages 2 TROUBLESHOOTING E 001 DILUTER INITIALIZE ERROR E 002 BLOOD SAMPLING ERROR E 003 DILUTER ERROR The diluter unit does not perform the initialization The diluter unit does not aspirate a blood sample System Error Message Possible Cause Criteria Countermeasure Replace the diluter unit with a new one Replace the DRIVER board with a new one E 021 SAMPLER INITIALIZE ERROR The sampler unit does not perform the initialization E 022 SAMPLER ERROR E 041 WBC SUB BATH INITIALIZE ERROR E 042 WBC SUB BATH ERROR E 051 RBC SUB BATH INITIALIZE ERROR The sampler unit does not work The WBC sub bath does not perform the initialization The WBC sub bath does not work The RBC sub bath does not perform the initialization E 052 RBC SUB BATH ERROR E 081 TRIPLE PUMP INITIALIZE The RBC sub bath does not work The triple pump unit does not perform the Replace the sampler unit with a new one Replace the DRIVER board with a new one Replace the C
121. logy Analyzer sesseeee 8 8 Adjusting Gain and Measuring Background Noise sssseeeeenenn 8 9 glo WENA E 8 9 Reference Principle of Differentiating WBC sssssssesseeeenn 8 9 Counting the Polymer Microsphere Suspensions esseeeeenee 8 10 Adjusting the Flow Cell Unit Position 8 12 Adjusting Gain FS and FL for WBC 5 Part Differential Parameters in Rough Mode Using Polymer Microsphere Suspensions sse 8 15 Measuring Background NoiSe ooooooocccccccnnococcccccnonencncnncnonnnnnononcnnnnnnnnnnncnnnnennnnnnnnnanens 8 16 Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous o bebe ued A a 8 17 Checking the Gain Adjustment ooooccccnnncccnonccccconnancncononancnno conca nn nn nano iiine i dienaa 8 20 Calibration micos as 8 21 Procedure Flowchart scimiscia aa aie REONE EEE ea 8 21 Calibration for CBC Paramters with Calibrator sseseeeene 8 21 Calibration for WBC 5 Part Differential Parameters oooocconnncccinnncccnnnccinonancnnnanancncnnor 8 26 eite deelBEicc lit a data eta aer 8 27 Checking Data with MEK 5D Hematology Control sssseeeeenee 8 27 Checking Data with Human Blood Samples sse 8 28 Interference Substances sssssssssssssssseeesee eene aE rnnt nnne a nennen nnn nnns 8 29 Storing and Transporting the Hematology Analyzer
122. m when an error message occurs There are two types of error messages Error message when turning the power on Error message related to measurement CAUTION When an alarm occurs the acquired data may not be correct especially when the or sample error message appears Do not use the alarmed data If the hematology analyzer detects an error the error message appears on the screen as shown below NOTE When performing the strong cleaning as the countermeasure after the strong cleaning is completed press the count switch without aspirating the diluent from the sampling nozzle Perform this procedure two or more times so that the diluent replaces the CLEANAC 3 detergent inside the hematology analyzer PARAMETERS 22 SAMPLING METHOD VENOUS SAMPLE TYPE BLOOD CLEAN KEY VERS ION V01 07 MENU ID ORDER RESULT 22 98 A 003 NO HEMOLYN Error message when turning the power on BLOOD VENOUS PSE ID uB cLos B B1L 1805 4 L g dL r4 fL PS g dL OS 16 21 1 e o E E VERSION EU 6 ER Se 2298 Error message after cleaning or priming CIN NNN Wu 188 L MENU 1D ORD ER ma ore Error message after measurement Service Manual MEK 7222 2 1 2 TROUBLESHOOTING Error Messages Erroneous operation during counting Messages Possible Cause Criteria Countermeasure A 001 NO DILUENT A 002 NO CLEANACe3 Out of diluent
123. ment leave the samples for more than 30 minutes before measurement When a sample is stored in a cool place such as a refrigerator or when the sample is left for more than 12 hours after collection it may affect WBC differential measurement Thoroughly mix the samples immediately before measurement If the blood sample is stirred too much so that bubbles are created the sample may be hemolyzed Do not measure coagulated samples Counting a coagulated sample may damage the hematology analyzer 8 17 8 DEMONSTRATION GUIDE 1 Count from several to about 15 human blood samples from different healthy people which are 30 minutes to 8 hours after collection 2 Display the AUTO DIFF GAIN screen MENU gt SETTINGS gt SENS amp THRESHOLD AUTO DIFF GAIN The number of data that The scattergram serial can be registered number The sample ID The date and time of measurement The number of The mark appears when the 1D cooonnnn 280 registered samples sample is registered on the DATE 12 FEB 03 ME 15 48 eak list P MO PEAK N 1 NOW MO peaks of the NOU BUE am displayed scattergram AVE The average peaks of the registered MO distribution AIM The target MO peaks Actual gain gt Optimum gain calculated by MO peak LIST va AUTO 1 Change gain to Return to the the optimum gain SENS amp THRESHOLD Go to the PEAK LIST screen screen Change samples to be displayed Register the di
124. n be used as a serial port Serial port 2 2 Bar code socket Also supplies the power to the bar code reader Power supply voltage 5 VDC pin 9 5 V pin 5 GND Rated current 200 mA 3 Printer socket Connects to an external printer WA 710V or other 4 Main power switch Supplies the power to the hematology analyzer when it is turned on Under normal conditions keep this switch turned on Contains the time lag fuse To replace the fuse contact your i Puce holder Nihon Kohden distributor 6 AC SOURCE Connects the AC power cord to supply AC power to the AC source socket hematology analyzer 7 Equipotential ground Connects the ground lead to the equipotential ground terminal on terminal the wall for earth grounding CAUTION e In order to avoid any safety hazard only connect personal computers which are approved by IEC 60950 using the information found in the reference in Section 10 e Only use the 3 prong power cord for the PC Service Manual MEK 7222 1 GENERAL Inside Panels CAUTION e For WBC 5 part differential measurement the laser beam is used Do not open any part labeled CAUTION The laser can cause burns and blindness e Do not remove the caution labels FECC AM t 7T4 4UVS O yD ERRTSE 25A1vL 7 85 L U7 3x5 cd E AEEHRRI URIEU USE CLASS 1 LASER PRODUCT CAUTION LASER RADIATION WHEN OPEN AND INTERLOCKS DEFEATED AVOID EXPOSURE TO BEAM
125. n the environmental temperature is 15 to 23 C temperature in the mix chamber is set to 36 3 C When the environmental temperature is 23 C or higher temperature in the mix chamber is set to 30 C Temperature in the mix chamber is fixed to 36 3 C ON When the environmental temperature is 15 to 23 C temperature in the mix chamber is set to 34 1 C When the environmental temperature is 23 to 28 C temperature in the mix chamber is set to 28 C Temperature in the mix chamber is fixed to 34 1 C Service Manual MEK 7222 7 5 7 Test Point Variable Resistor LED and Switch on Board TRIPLE PUMP board D014 D015 D016 D017 D018 D011 D012 D013 D011 HCPHI LED for operation check of the high position sensor When the sensor is turned on this LED is lit D012 HCPMID LED for operation check of the middle position sensor When the sensor is turned on this LED is lit D013 HCPLOW LED for operation check of the low position sensor When the sensor is turned on this LED is lit D014 MV 1 LED for operation check of the MV1 valve When the valve is turned on this LED is lit D015 MV2 LED for operation check of the MV2 valve When the valve is turned on this LED is lit D016 MV3 LED for operation check of the MV3 valve When the valve is turned on this LED is lit D017 MV4 LED for operation check of the MV4 valve When the valve is turned on this LED is lit D018 MV5 LED for
126. nalyzer 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Loosen the 2 black screws which secure the inlet outlet unit to the chassis 5 Release the tube tie as shown below and disconnect the No 9 tube joint No 9 4 22 Service Manual MEK 7222 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 6 Disconnect the No 15 tube joint as shown below No 15 7 Disconnect the 5 tube joints No 10 to No 14 No 13 No 12 No 11 No 10 No 14 4 23 4 DISASSEMBLY AND ASSEMBLY 9 Slightly pull the inlet outlet unit upward and move it rightward Completely remove the inlet outlet unit To assemble the hematology analyzer reverse the above procedure Removing the Flow Cell Adjuster 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Remove the 2 black screws which secure the flow cell adjuster to the chassis 4 24 Service Manual MEK 7222 4 DISASSE
127. nalyzer wait approx 10 minutes after turning off the main power switch 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Insert a small flat blade screwdriver between the ROM and its socket on the AMP CONTROL board Carefully lift the ROM with the screwdriver until the ROM slightly tilts Take out the screwdriver and insert it into the oppisite side of the IC socket Continue lifting both sides of the ROM with the screwdriver until the ROM is removed CAUTION When you remove the ROM from the IC socket with a sharp edge tool such as a flat blade screwdriver be sure that it does not damage the printed pattern under the IC socket 4 DISASSEMBLY AND ASSEMBLY 4 10 5 Put the new ROM on the IC socket and carefully insert the pins of the new ROM into the IC socket CAUTION Before putting the new ROM on the IC socket check that the ROM position is correct as shown in step 4 Do not break or bend a pin of the ROM The pins must be correctly inserted into the socket Otherwise the hematology analyzer may not work 10 11 Put the top cover back to the original position Refer to Removing the Top Cover Turn on the main power switch on the rear panel Continue pressing the Reset key Clean key and Dispense Open key on the front panel press the Power key on the panel release the Power key and release the Reset key Clean key and Dispense
128. nd close the door tube holder To measure WBC 5 part differential parameters set the laser switch on the right side panel to on with the laser key A 090 ROOM TEMP OVER A 091 ROOM TEMP LOW Measurement is performed at temperatures over 30 C Measurement is performed at temperatures below 15 C Perform measurement at temperatures between 15 and 30 C A 093 CASE TEMP LOW A 094 POWER TEMP OVER A 101 WA 720VK ERROR A 092 CASE TEMP OVER Internal temperature especially at the sheath unit is abnormal Internal temperature especially at the power supply unit is abnormal The thermal head of the built in printer is not ready for printing Turn off the hematology analyzer After ventilating it turn it on Check the operation Replace the sheath unit with a new one if the message still appears after improving the ventilation Turn off the hematology analyzer After ventilating it turn it on Check the operation Set the head on the paper with the head set lever Replace the printer with a new one if the message still appears after setting the head on the paper A 102 NO PAPER There is no paper in the built in printer Load the paper properly A 103 WA 710V ERROR The locally purchased external printer is not ready for printing Check the printer according to the instruction manual of the printer A 104 NO PAPER There is no paper in the locally pu
129. nd perform grounding if necessary Turn the laser switch on the right side panel to on for WBC 5 part differential measurement Turn on the power of the hematology analyzer Connect the following reagent bottles and waste container Waste container Diluent ISOTONACs3 Detergent CLEANAC 3 Detergent CLEANAC Hemolysing reagent HEMOLYNAC 3 or HEMOLYNAC 3N Hemolysing reagent HEMOLYNAC 5 Tubes and caps necessary for connecting the reagents and container and the inlets and outlets to which these reagents and container are to be connected are indicated in the following illustration and table For details refer to the operator s manual CAUTION Avoid reagents or waste fluid contact with the skin If it contacts the skin or eyes or is swallowed wash thoroughly with water and see a physician immediately NOTE Be careful not to let dust get in the hemolysing reagent diluent or detergent When using the diluent container detergent container or waste container follow the instructions on each package Place the diluent and detergent containers at the same level as the hematology analyzer Do not squeeze or bend the tubes Otherwise the hematology analyzer may be damaged If necessary cut the tube to an appropriate length if it does not fit Only use the specified detergent tubes for the detergent When using HEMOLYNACe3N reagent after using HEMOLYNAC 3 reagent calibrate the hematology analyzer after changing the reag
130. nit There are two rotary switches on the PRE AMP board Each rotary switch selects one of 10 terminals The rotary switch for the first digit of the two digit compensation value has 0 2 increment or decrement per terminal The rotary switch for the second digit of the two digit compensation value has 2 increment or decrement per terminal 5 3 5 Adjustment When the WBC RBC and PLT data have 2 decreases with the hematology control after replacing the manometer with a new one change the compensation value with the rotary switches so that the WBC RBC and PLT data have 2 increase If the first digit and second digit rotary switches are set to 3 and 5 respectively the compensation value is 53 To increase the WBC RBC and PLT data decrease the compensation value In this case to have 2 increases at WBC RBC and PLT data change the compensation value 53 to 43 because 2 0 2 10 Therefore set the second digit rotary switch to 4 If you change the compensation value from 53 to 60 the WBC RBC and PLT data have 1 4 decrease because 7 x 0 2 1 4 Adjusting Gain for WBC 5 Part Differential Measurement 5 4 When the scattergrams appear outside their allotted area on the screen or the flags frequently appear adjust the gain for WBC 5 part differential measurement Two steps must be performed First adjust the gain roughly by measuring 7 um polymer microsph
131. nt the polymer microsphere suspensions Use the CANCEL key to return to the OPERATIONS screen MANUAL ADJUSTMENT 12 FEB 03 13 29 FS FL 60 COUNT 0 hos GE POSITION CVS 0 0 0 0 NOW START gt PEAK 0 0 5000 5000 CANCEL PRESS COUNT KEY TO START 8 13 8 DEMONSTRATION GUIDE 4 Put the sampling nozzle into the bottom of the sample container containing the polymer microsphere suspensions so that the tip of the sampling nozzle comes near but does not touch the bottom of the sample container NOTE Do not let the sampling nozzle touch the bottom of the sample container This may prevent aspiration of the polymer microsphere suspensions fol Put the sampling nozzle to this level 5 Press the Count switch The polymer microsphere suspensions in the sample container are aspirated and measurement starts The MEASURING PARTICLE message appears on the screen and the scattergrams and histograms appear in real time The measurement takes approximately 40 seconds MANUAL ADJUSTMENT 12 FEB O3 13 29 oO B COUNT 707 POSITION FS FL CY 23 8 13 3 NOW START PEAK 44 76 5000 5000 AR MEASURING PARTICLE Adjust the flow cell FS histogram CV and PEAK values of FS and FL are displayed The histogram is redrawn without unit adjusting the flow cell unit 6 Press the or key to adjust the flow cell unit position The histogram is automatically redrawn A
132. nts priming and circuit self check are automatically performed After priming operation is completed the READY screen appears The hematology analyzer is ready for counting 26 AUG 63 15 19 ID 0001 PARAMETERS 22 SAMPLING CLOSED SAMPLE TYPE BLOOD VERSION V02 01 8 7 8 DEMONSTRATION GUIDE Setting the Date 8 Time and Cleaning the Hematology Analyzer Checking the Date and After turning the hematology analyzer power on check that the date and time Time Settings displayed at the upper right of the screen are correct ii ID 0001 Display the DATE amp TIME screen MENU gt SETTINGS gt DATE amp TIME Select the setting item by pressing the item key The cursor moves to the item 14 FEB 03 15 13 03 J VALUEL Item keys End EER N Eq 1 Numeric keypad 14 3 Enter the value using the numeric keys The entered value appears next to 4 5 6 Cleaning the Fluid Path in 1 the Hematology Analyzer 2 8 8 VALUE Press the ENTER key to register the value The cursor moves to the next item Repeat steps 2 to 4 to enter other items Press the OK key to finish setting and return to the SETTINGS screen The clock starts immediately from the new date and time Clean the fluid path in the hematology analyzer by pressing the clean key yy on the front panel os SEP 03 69 15 CLEANING PLEASE WAIT The READY screen appears after cleaning It takes about 12 minu
133. o replace faulty units printed circuit boards or parts We do not support component level repair of boards and units outside the factory NOTE e When ordering parts or accessories from your nearest Nihon Kohden Corporation s distributor please quote the NK code number and part name which is listed in this service manual and the name or model of the unit in which the required part is located This will help us to promptly attend to your needs e Always use parts and accessories recommended or supplied by Nihon Kohden Corporation to assure maximum performance from your instrument Service Manual MEK 7222 Specifications 1 GENERAL Measured Parameters Ranges and Reproducibility to Specimen from Venous Blood Specifications except WBC population were determined using hematology control blood MEK 3DN counted 10 times consecutively Measured Parameters WBC White blood cell count Measuring Range CV Coefficient of Variation 0 to 300 x 10 uL Reproducibility to Specimen from Venous Blood within 2 0 CV 4 0 to 9 0 x 10 uL NE Neutrophil percent LY Lymphocyte percent MO Monocyte percent EO Eosinophil percent BA Basophil percent NE Neutrophil count 0 to 99 9 0 to 99 9 0 to 99 9 0 to 99 9 0 to 99 9 0 to 99 9 x 107 uL within 5 0 CV WBC 4 0 to 9 0 x 10 UL NE 40 to 70 within 5 0 CV WBC 4 0 to 9 0 x 10 uL LY 20 to 45 within 12 0 CV WBC 4 0 to 9 0 x 107 uL
134. ode Using Venous Blood Ezr go e PU Y 8 17 Checking the Gain Adjustment ooooccccnnnccccnnaaccnonnncnnonnnnanonnnnnnnnno carr nnn nana nnne nnns 8 20 Service Manual MEK 7222 CONTENTS er lloro rec 8 21 Procedure Flowchakt ceu A Ei 8 21 Calibration for CBC Paramters with Calibrator sssee 8 21 Calibration for WBC 5 Part Differential Parameters ssssseseeeeeeee 8 26 Che king Data sis m 8 27 Checking Data with MEK 5D Hematology Control sse 8 27 Checking Data with Human Blood Samples ssssssseeeeeenne 8 28 Interference Substances iii 8 29 Storing and Transporting the Hematology Analyzer sssssseeeee 8 32 MEK 7222J K Hematology Analyzer Installation Check Sheet 8 34 Service Manual MEK 7222 C 5 CONTENTS This page is intentionally left blank C 6 Service Manual MEK 7222 EMC RELATED CAUTION This equipment and or system complies with the International Standard EN61326 1 for electromagnetic compatibility for electrical equipment and or system for measurement control and laboratory use However an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326 1 can cause harmful interference to the equipment and or system or cause the equipment and or system to fail to perform its intended function or degrade its intended
135. old of the sheath unit Service Manual MEK 7222 4 29 4 DISASSEMBLY AND ASSEMBLY 11 Remove the 2 black screws which secure the flow cell unit holder 12 Move the holder leftward and downward and remove it from the laser optical unit 13 Disconnect the 2 PharMed tubes from the single manifold Manifold 2 PharMed tubes 14 Pull the sheath unit upward and pull it toward you CAUTION When removing the sheath unit from the chassis check that there is no damage on the tubes and cables connected to the sheath unit To assemble the hematology analyzer reverse the above procedure 4 30 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Removing the Rinse Unit and Switch Assembly 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Disconnect the 2 tube joints connected to the rinse unit 5 Release the tube tie which binds the 2 tubes Remove the 4 screws which secure the rinse unit and switch assembly to the chassis 7 Disconnect the white connector of the assembly as shown below To assemble
136. on Board HGB AMP board TP052 TPO51 VRO51 TPOS1 EA Ground terminal for analog circuit TPO52 HANA Test point for output voltage from HGB sensor VROSI HGB VR Variable resistor for adjustment of output voltage from HGB sensor MANOMETER board D041 VR041 VR042 D042 TP041 TP042 TP043 TP041 UPE Test point for output voltage from the optical sensor at the upper part of the manometer TP042 LOWE Test point for output voltage from the optical sensor at the lower part of the manometer TP043 ED Ground terminal for digital circuit VRO41 UP VR Variable resistor for adjustment of output voltage from optical sensor at the upper part of the manometer VRO042 LOW VR Variable resistor for adjustment of output voltage from optical sensor at the lower part of the manometer D041 UPER LED for operation check of optical sensor at the upper part of the manometer When the fluid level is higher than the upper sensor this LED is lit D042 LOWER LED for operation check of optical sensor at the lower part of the manometer When the fluid level is higher than the lower sensor this LED is lit 72 Service Manual MEK 7222 7 Test Point Variable Resistor LED and Switch on Board MV CONNECTION board MV8 MV9 MV1 MV2 MV3 MV4 MV5 MV6 MV7 D061 MV1 LED for operation check of MV1 valve When the MV1 works this LED lights D062 MV2 LED for operation check of MV2 valve When the MV2
137. operation check of the MV5 valve When the valve is turned on this LED is lit 7 6 Service Manual MEK 7222 7 Test Point Variable Resistor LED and Switch on Board DILUTER DRIVER board Service Manual MEK 7222 TPO11 ORIGIN Test point for output voltage from the home position sensor for the piston of the diluter TPO12 ENCODE Test point for output voltage from the position encoder for the piston of the diluter TPOI3 ED Ground terminal for digital circuit TP014 5V Test point for 5 V DC for digital circuit SWOII DIL ADJ 2 Switch for compensation of the diluter This switch is used for the first digit of the two digit compensation value SWOI2 DIL AD J 1 Switch for compensation of the diluter This switch is used for the second digit of the two digit compensation value DO019 ENCODE LED for operation check of the position encoder for the piston of the diluter When the encoder is turned on this LED is lit DO110 ORIGIN LED for operation check of the home position sensor for the piston of the diluter When the sensor is turned on this LED in lit D0111 MD1MV LED for operation check of the MV1 valve When the valve is turned on this LED is lit D0112 MD2MV Reserved LED for operation check of the MV2 valve When the valve is turned on this LED is lit 7 7 7 Test Point Variable Resistor LED and Switch on Board SAMPLER X board SAMPLER Z board 7 8 D011 D012 D013 D017 D02
138. osuppressive drugs may increase the fragility of cells which may cause low PLT count In such a case measure manually Hemolysis Hemolyzed specimens contain red cell stroma which may increase PLT count Anticoagulated blood Blood anticoagulated with acid citrate dextrose may have clumped PLT which may cause decrease in PLT count Agglutinated PLT Clumped PLT may decrease PLT count and or increase WBC count For such sample collect the sample in sodium citrate anticoagulant and measure only PLT The PLT result must be corrected for the sodium citrate dilution effect Very large PLT Large PLT may exceed the PLT threshold and not be counted which results in low MPV Very small fragments Very small RBC RBC fragments and WBC fragments may interfere with MPV measurement Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting erroneous MPV This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Chemotherapy Cytotoxic and immunosuppressive drugs may affect MPV In such a case measure manually NOTE Blood samples collected in EDTA does not maintain stable MPV because platelets swell depending on the interval after collection and storage temperature Service Manual MEK 7222 1 5 1 GENERAL WBC 5 part differential parameters are derived from the WBC count therefore the limitations for WBC also affect these parameters LY and LY Erythroblasts certain parasites and RBC th
139. pe e Tweezers 4 DISASSEMBLY AND ASSEMBLY Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the Instrument 4 2 CAUTION Valve Joint When connecting the valve joint to the electromagnetic valve turn the valve joint clockwise using moderate force until the valve joint comes to a stop Do not use extreme force to tighten the valve joint further because this will damage the tip of the valve joint If the valve joint is loosely connected it will leak NOTE Black screw Black screws are used to fasten the individual units to the chassis of the instrument to enable the quick removal and replacement of these units However to fasten the pump unit to the chassis normal screws are used Spring type tube joint e Spring type tube joints are used in the instrument to prevent over tightening of the joints and to prevent loosening of the joints after the joints are tightened e There are 2 types of spring tube joints white inlet side and black outlet side Each tube joint and its corresponding port in the instrument are marked with the same color or number to ensure matching Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY No spring type tube joint The no spring type tube joint consists of the O ring tube stopper and fitting nut To disconnect the tube from this joint turn the fitting nut counterclockwise to loosen the joint and pull the tube toward you To reconnect the tu
140. placing the Sampling Nozzles sseem nr 6 19 Service Manual MEK 7222 C 3 CONTENTS Section 7 Section 8 CA Checking and Cleaning the Cap Pierce Rinse Unit Sampling Nozzle and Cap Pierce Needle Optional MS 721V Cap Pierce Unit sssseeese 6 21 Checking and Replacing the Cap Pierce Needle Optional MS 721V Cap Pierce Unit 6 23 e7 Lelr uineB oU eiicie me e M 6 25 Maintenance Check Sheet c ccccescccesesseeeececeeeeeeceeseeaeeeeeeaeeeeaeeeeseaeeseeneaeeseeaeeeeenaaeeees 6 26 Test Point Variable Resistor LED and Switch on Board 7C 1 PREAMP SO ANG ecc 7 1 HGB AMP board i e ec ec hatin ee ih ab a ee 7 2 MANOMETER Oar as ione c dnd aa 7 2 MV CONNECTION board isseire tata 7 3 AWAY LIQUID SENSOR board 1 rre eai rece td honte de dd nea unas 7 4 SHEATH DOIG ERE C I RS 7 5 TRIPEE PUMP boardcode incida 7 6 DILUTER DRIVER DOGIG ii et titi T SAMPLER X board EE 7 8 SAMPLER Z board ci alta 7 8 KEY DISPLAY DOAEGQ iioii recede pne c etes ene eee taa 7 9 eB 7 9 eidem Banus a EN 7 10 AMP CONTROL board coccccnoccccnnoncccnnoncnnononcnnnnnnnnnnnn anaa aa aaa aaan a Ra ea inneren nnns 7 12 DRIVER DOTA aisiais oi a E E E E O Eak 7 14 Demonstration Guide 00 8C 1 INTO UCHON sr AI 8 1 Demonstration Outline oooonocccnnnncccnnoncccnonocanononancnana cnn nana ncn none n cn naar cnn naar cnn naar n na
141. r is installed Pressing the Feed key on the front panel feeds the recording paper and there is no dot missing on the paper by pressing the Print key on the front panel Yes No External Printer Unit Operation When the external printer is connected Paper feed operation properly works on the external printer and there is no dot missing on the paper Yes No Bar Code Reader Operation When the bar code reader is installed Light emitter and detector parts are cleaned with a cotton swab moistened with 80 alcohol Yes No Bar code reading operation properly works Yes No Others Write down any other points on this sheet Service Manual MEK 7222 aJey mo Section 7 Test point Variable Resister LED and Switch on Board PRE AMP DO ice TED TM 7 1 HGB AMP Doard Me MES 7 2 MANOMETER DOM idos 7 2 MV CONNECTION board cooococooocnccccccccccnnnnccncccnccnnonononnnonononononnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccnnnnos 7 3 AWAY LIQUID SENSOR DOALrd 2 epoca ba yi veo coda decenas ee vest alacena 7 4 SHEATH DO rd seur isis 7 5 TRIPLE PUMP board m Edi 7 6 DILCUTER DRIVER DOard a omen erint ida 7 7 SAMPLER XDOard ttt dire re tasa fucci is 7 8 SAMPEER Z DOANA A e 7 8 KEY deli dosi aditld 7 9 jue NBI Iioc 7 9 POWERDOS Unai ii eee 7 10 AMP CONTROL DOA rd ot os 7 12 B zibdzzdse ip iii 7 14 Service Manual MEK 7222 7C 1 7 Test Point Variable Resistor LE
142. rare occasions the RBC in the blood sample may not completely lyse and these non lysed RBC may be detected as WBC and cause increase in WBC count Multiple myeloma The precipitation of proteins in multiple myeloma patients may increase the WBC count Leukemia WBC is fragile in leukemia patients and WBC may be destroyed during measurement These WBC fragments may also interfere with WBC differential measurement Chemotherapy Cytotoxic and immunosuppressive drugs cause low WBC count Cryoglobulins Cryoglobulin may be increased in patients who are pregnant or have myeloma cancer leukemia macroglobulinemia lymphoproliferative disorders metastatic tumors autoimmune disorders infections aneurysm thromboembolic phenomena diabetes etc which cause increase in WBC RBC or PLT counts and HGB concentration In such cases warm the blood sample to 37 C in a water bath for 30 minutes and measure the sample immediately Leukemia An increase in WBC in leukemia patient causes increase in RBC Agglutinated RBC Agglutinated RBC may decrease RBC count This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Cold agglutinins IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and increase MCV Turbidity of the blood sample Any physiologic and or therapeutic factors may increase HGB In such a case determine the cause of turbidity and follow the appropriate method below 1
143. rating humidity 30 to 85 Non condensing Storage atmospheric pressure 70 to 106 kPa Operating atmospheric pressure 70 to 106 kPa Power Requirements Power requirements MEK 7222J 110 117 or 127 V 10 AC 50 60 Hz MEK 7222K 220 230 or 240 V 10 AC 50 60 Hz Power consumption 250 VA 1 6 Service Manual MEK 7222 1 GENERAL Dimensions and Weight Dimensions 382 W x 485 D x 520 H mm Net weight approx 38 kg Electromagnetic Compatibility IEC 61326 1 1997 Amendment 1 1998 Amendment 2 2000 EN 61326 1 1997 Amendment 1 1998 CISPR11 1997 Group 1 Class B EN 55011 1998 Amendment 1 1999 Group 1 Class B The power supply short interruption test is performed through the transformer which has three times or more as large capacity as the instrument Safety Safety standards IEC 61010 1 2nd Edition 2001 EN 61010 1 2nd Edition 2001 Laser IEC 60825 1 1993 Amendment 1 1997 Class 1 EN 60825 1 1994 Amendment 11 1996 According to the type of protection against electrical shock CLASS I EQUIPMENT According to the degree of protection against harmful ingress of water IPXO Ordinary EQUIPMENT According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR OR WITH OXYGEN OR NITROUS OXIDE EQUIPMENT not suitable for use in the presence of FLAMMABLE ANAESTHETIC MIXTURE WITH AIR OR WITH OXYGEN OR NITROUS OXIDE According to the mode of operation CONTINUOUS O
144. rchased external printer Load the paper properly according to the instruction manual of the printer Service Manual MEK 7222 2 3 TROUBLESHOOTING Error Messages Possible Cause Criteria Countermeasure WBC 5 0 RBC 4 50 is displayed on the right of measured value HGB 13 4 is displayed on the right of HGB measured value Abnormal sample The WBC measurement bath is dirty Check that the temperature of the diluent is between 15 and 30 C Then recount the sample Water and alcohol cannot be measured Clean the measurement bath HGB 13 4 is displayed on the right of HGB measured value MCHC 38 0 is displayed on the right of MCHC measured value WBC 9 0 is displayed on the right of WBC measured value HGB circuit error Abnormal RBC Error occurred when diluting the sample WBC counting error Poor hemolyzation HGB 13 4 is displayed on the right of HGB measured value HGB voltage adjustment error WBC 9 0C PLT 490C C is displayed on the right of WBC and PLT measured values Replace filters Replace pump tubes Check cap pierce needle WBC and PLT counting error Platelet coagulated Poor hemolyzation and low PLT Measurement is performed more than 1000 times Measurement is performd more than 3000 times The measurement is performed more than 1000 times in closed mode when the MS 721V cap pierce unit is insta
145. rd KEY DISPLAY Board and LCD Unit 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Remove the front panel unit according to the procedure described in Removing the Front Panel Unit 3 Remove the 12 screws which secure the optional printer unit and shield cover to the front panel unit Remove first the printer unit and second the shield cover from the front panel unit CAUTION Pay attention to the sharp edges of the shield cover lt can cause cuts or injuries To reduce these possibilities wear gloves Shield cover Printer unit 4 34 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 4 To remove the VOLUME board disconnect the cable 1 at the KEY DISPLAY board and remove the 2 screws screws and 2 from the VOLUME board Screw 3 Screw 12 Cable 3 LCD unit chassis Screw 11 Screw 4 Cale Screw 2 Screw 13 Screw 1 Screw 5 Screw 10 Screw 16 Screw 6 Cable 1 Screw 14 Secrew 9 Screw 8 Screw 15 Screw 7 Cable 4 NOTE The VOLUME board is not available If this board has a problem only the variable resistor will be sent to you for replacement 5 Toremove the KEY DISPLAY board disconnect the 4 cables cables 1 to 4 from the board and remove the 8 screws screws 3 to
146. rding to the procedure described in Opening the Front Cover of Section 4 Disassembly and Assembly Remove the screw which secures the HGB cover to the CBC measuring unit chassis Remove the HGB cover Screw TP052 HANA VRO51 HGB VR TP051 EA Fill the sub bath for the WBC measurement bath with diluent Press the OTHER key on the MENU screen The OTHER screen appears Press the SENSOR MONITOR key on the OTHER screen The SENSOR MONITOR screen which displays each sensor output voltage appears Adjust the VRO51 HGB VR variable resistor on the HGB board so that the HGB LED ON voltage is 3 V 0 1 V Connect a digital voltmeter between the TP051 EA and TP052 HANA test points and check that the voltage between the TP051 and TP052 test points is 3 V 0 1 V If the voltage is out of the range adjust the VRO51 HGB VR variable resistor for 3 V 0 1 V NOTE If the output voltage from the sensor is less than before when the diluent is aspirated through the aperture cap it does not affect the HGB measurement value but the resolution for HGB measurement decreases Refer to Hemoglobin Measurement in Section 10 Reference of the operator s manual 5 1 5 Adjustment NOTE in Assembling the Instrument Before closing the front cover check that the HGB cover is attached to the original position Adjusting Upper and Lower Sensor Output Voltage of the Manometer Manometer window D041 UPPER VRO041
147. re not many scattergrams in the upper right and central area MO PEAK check point AVERAGE PEAK and IDEAL PEAK are the same Check the values AUTO DIFF GAIN 12 FEB O3 15 41 FS AVE 186 to 192 D31 031 FL AVE 47 to 51 SD AVE 21 to 23 j E ID 0000000001230 8 20 DATE 12 FEB 03 TIME 15 40 MO PEAK N D NOW AVE AIM GAIN FS 722 FL 62 61 SD 20 30 If the measured data is not optimum perform the procedure in the Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous Blood Samples section again NOTE The measured data is not optimum if the human blood sample used in the above measurement is abnormal or the gain was adjusted greatly in the adjustment in fine mode Service Manual MEK 7222 Calibration Procedure Flowchart Calibration for CBC Parameters with Calibrator Service Manual MEK 7222 8 DEMONSTRATION GUIDE 1 Perform calibration measurement with MEK 3DN calibrator to obtain a mean value for calculating the new calibration coefficient 2 Perform the calibration for CBC parameters WBC RBC HGB MCV PLT RDW MPV using the MEK 3DN calibrator 3 Check that the calibration coefficients for WBC 5 part differential parameters LY MO EO BA are 1 000 on the CALIBRATION screen In the gain adjustment performed in the previous section WBC 5 part differential parameters are already accurately calibrated Therefore you only need to check the calibr
148. rward small angle scatter FL forward large angle scatter SD side scatter From the forward small angle scatter the size of the cell is detected From the forward large angle scatter the complexity of the cell is detected From the side scatter the granularity of the cell is detected 8 9 8 DEMONSTRATION GUIDE Counting the Polymer Microsphere Suspensions OPERATIONS 14 FEB 03 15 21 PRIME DRAIN BATHS AUTO PRIME DRAIN ALL STRONG CLEAN PARTICLE TEST GEN FLOWCELL ADJUST FLONCELL Gz PARTICLE TEST 12 FEB OS 14 05 FS EE cuU 0 0 0 0 0 0 PEAK Cm COCOS MEASURING PARTICLES 8 10 eb Put the sampling nozzle to this level Count the polymer microsphere suspensions YZ 0194 supply code no T905 to check the CV and PEAK values 1 Clean the flow cell to remove dust and bubbles inside the flow cell Press the CLEAN FLOWCELL key on the OPERATIONS screen to clean the flow cell 2 Prepare 1 mL of polymer microsphere suspensions in a sample container 3 When the MS 721V cap pierce unit is installed select OPEN mode on the ORDER screen 4 Display the PARTICLE TEST screen MENU 3 OPERATIONS PARTICLE TEST 5 Press the MEASURE key The PRESS COUNT KEY TO START message appears and the hematology analyzer is ready for counting the polymer microsphere suspensions CUM 0 0 0 0 0 8 NEN PEAK a a CANCEL PRESS COUNT KEY TO START 6 Put the sampling nozzle into
149. s Cautions and Notes sesssssssssssseeeeeenneennnn nennen nennen nnn iii Explanations of the Symbols in this Manual and Instrument ssessssss iv 97 of A tee erie ere ee een eee reper Perrier ete errr ere er errr E T iv On screen and recorded data ooooocconoccccconccccconcnccnoncnnnnnnnncnnannncannnnn narran cn ennt V D 1C 1 INTOdUCION Bem cR 1 1 SEVICE Rol eiii iaa 1 2 Specifications iii na 1 3 Measured Parameters Ranges and Reproducibility to Specimen from Venous Dl EET 1 3 Detection MetMoOd id UR nas iene eu de reli Bateau 1 3 Standardization Analysis Method ssssseeeeenennn 1 3 Interference Substances iii ai 1 4 Dilution AO pican indir 1 6 Counting TME e LS iia 1 6 Br 1 6 Data Storage sesssssssssssssseeseeeeee nennen nennen cnn rra 1 6 Environmental Conditions eese nnns 1 6 Power hequlremliehts ccce lao Cute tabo ipd ete DEL oue a 1 6 Dimensions and Weight ssssssseeeeneeem eene nnn 1 7 Electromagnetic Compatibility isss esisiini taiias 1 7 rM EET 1 7 Panel IDESCHIPU OMS ft PL dui 1 8 Iuuen emo 1 8 Right Side Panel sese eene nennen aR EREE Enae oiana EEn 1 9 REA RAMEN T TETTE RI UTI UNE 1 10 Inside Panels its adi 1 11
150. s the control data from the AMP CONTROL board and drives the valves and motors by setting the drive current to on or off Optional Cap Pierce Unit MS 721V Cap pierce needle Cap pierce rinse unit Tube holder door Count switch The cap pierce unit allows you to get the measurement data without opening the cap of the sample tube and touching the blood 3 8 Service Manual MEK 7222 Section 4 Disassembly and Assembly Before YOU BODIN etica de dran exer a De uid p 4 1 Warnings and Cautions sse eene n nnns nennen nnns 4 1 Required TOOIS C 4 1 Caution and Notes Related to Valve Joint Black Screw and Tube Joint in the Iris UUM Sciacca costes erento dre tpud dde 4 2 Boardiand Unit LOCION cursar carl a eo gea comae duae ele rue Cota dodo 4 3 allele EE E 4 3 E anne ERAN a nied 4 3 Upper Part of Right Side niteat diee inerte a dedos b eed 4 4 Lower Partof Right Side inire Ex SERRE SR ONE ER LE EX RR Ree FE a 4 4 Front ViewWw 0f eic 4 4 Draining the Hematology Analyzer sssseseeeeennnm eene nnns 4 5 Opening the Front Cover ER 4 7 Removing the Top COve rs es sis seve a 4 8 Replacing the Boot ROM ccoo pili 4 9 Removing the Rear Cover Including the Power Supply Unit sssesesss 4 11 Removing the CBC Measuring Unit sssssssssseeeeeene eene 4 12 Removing the Dil ter Unit cia E tete tu piste Le
151. se the above procedure Service Manual MEK 7222 47 4 DISASSEMBLY AND ASSEMBLY Removing the Top Cover 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your safety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Remove the 6 screws which secure the top cover to the hematology analyzer chassis at the right and left side 7 7 sr E al P IN j p A LE Jm iih i TIT A B d 3 Slide the top cover rearward and remove it from the chassis To assemble the hematology analyzer reverse the above procedure NOTE in Assembling the Hematology Analyzer There are hooks at the top right and left inside of the top cover Fit these hooks into the front cover edge 4 8 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY Replacing the Boot ROM Service Manual MEK 7222 CAUTION The ROM is easily damaged by static electricity Before touching the ROM touch the metal chassis to eliminate static electricity from your body If there are different settings from the default settings on the CALIBRATION and SETTINGS screens write down these settings P Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy a
152. service maintain and repair the MEK 7222 Hematology Analyzer referred to as the instrument in this service manual The maintenance must be periodically performed because the instrument has fluid paths and precision parts Accordingly the user is responsible for performing the periodic maintenance The Maintenance section in this service manual describes the maintenance that should be performed by qualified service personnel The Maintenance section in the operator s manual describes the maintenance that can be performed by the user NOTE If the instrument has a problem and there has been no periodic maintenance the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one The information in the operator s manual is primarily for the user However it is important for service personnel to thoroughly read the operator s manual and service manual before starting to troubleshoot service maintain or repair this instrument This is because service personnel needs to understand the operation of the instrument in order to effectively use the information in the service manual 1 1 1 GENERAL Service Policy 1 2 CAUTION e Be careful not to directly touch any place where blood is or may spread to e Wear rubber gloves to protect yourself from infection before doing maintenance Nihon Kohden Corporation s basic policy for technical service is t
153. sodium citrate anticoagulant and measure only PLT The PLT result must be corrected for the sodium citrate dilution effect Very large PLT Large PLT may exceed the PLT threshold and not be counted which results in low MPV Very small fragments Very small RBC RBC fragments and WBC fragments may interfere with MPV measurement Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in erroneous MPV This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Chemotherapy Cytotoxic and immunosuppressive drugs may affect MPV In such a case measure manually NOTE Blood samples collected in EDTA do not maintain stable MPV because platelets swell depending on the interval after collection and storage temperature Service Manual MEK 7222 8 DEMONSTRATION GUIDE WBC 5 part differential parameters are derived from the WBC count therefore the limitations for WBC also affect these parameters NE and NE Excessive eosinophils metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate NE count and NE LY and LY Erythroblasts certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count MO and MO Large lymphocytes atypical lymphocytes blasts and excessive number of basophils may interfere with an accurate MO count EO and EO Abnormal granules may interfere with an accurate EO count BA and BA Immature cell metamyeloc
154. splayed sample on the peak list 3 Check if the sample is appropriate to use in gain adjustment Only register appropriate samples for gain adjustment Check Points of the optimum sample for adjustment e The distributions of NE LY and MO are oval in shape on the scattergram e The distributions of NE LY and MO are separate from each other e NE LY and MO should be located in the allotted positions Optimum scattergram for gain adjustment MO 8 18 Service Manual MEK 7222 8 DEMONSTRATION GUIDE Scattergrams which cannot be used for automatic gain adjustment FS GAIN too high a There is no NE distribution and MO and LY are not separated on the scattergram Scattergram of a leukemia patient b MO is distributed on NE Scattergram of a patient with immature granulocytes c NE is distributed too close to MO Scattergram of a blood sample collected more than 8 hours ago The number of i registered samples Registered mark 3 P 4 Press the ADD key to register the blood sample to the peak list is AUTO DIFF GAIN Testis displayed in the scattergram serial No after registration and the number of N P in the MO PEAK table is incremented Up to 12 samples can be registered ID You should have at least 5 samples registered Press the and keys to MO PEAK change displayed samples NOW UE m 2 pray P NOTE The mean value AVE of the registered MO distribution peak data is x 2 40
155. tes for cleaning Perform cleaning again by pressing the Clean key Clean the fluid path more than 2 times Service Manual MEK 7222 8 DEMONSTRATION GUIDE Adjusting Gain and Measuring Background Noise Flowchart Reference Principle of Differentiating WBC Laser Count the polymer microsphere suspensions to check the CV and PEAK values Adjust the flow cell unit position Adjust gain for WBC 5 part differential parameters in rough mode using polymer microsphere suspensions Measure background noise Adjust gain for WBC 5 part differential parameters in fine mode using venous blood samples from different healthy people which are 30 minutes to 8 hours after collection Check the actual peak list for all samples Make sure the average peak of MO 1s optimum The hematology analyzer uses the light scatter technique to differentiate WBC into neutrophil lymphocyte monocyte eosinophil and basophil counts The diluted blood sample is injected into the flow cell Blood cells pass through the sensing zone one by one A laser beam through the sensing zone is scattered by the passing cells and the scattered light is detected The angle and intensity of scattered light indicates the volume and characteristics of the cell From this WBC is differentiated into 5 parts Diluent Diluent Sample Service Manual MEK 7222 In gain adjustment you adjust the following FS fo
156. th sides are released Pull the sampler unit toward you 8 Disconnect the 2 cables from the sampler unit 9 Completely remove the sampler unit from the chassis To assemble the hematology analyzer reverse the above procedure CAUTION in Assembling the Hematology Analyzer Check that all the tube joints are firmly connected to the original positions Otherwise fluid may leak Removing the Pump Unit There are two pump units at the front of the hematology analyzer chassis To remove one of the pump units perform the following procedure 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy Analyzer 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the rear cover including the power supply unit according to the procedure described in Removing the Rear Cover Including the Power Supply Unit 4 16 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 4 Remove the 3 screws which secure the pump unit to the chassis The following picture shows the right pump unit about to be removed 5 Disconnect the motor cable of the pump unit at the DRIVER board on the rear cover Motor cable of left pump unit Motor cable of right pump unit To assemble the hematology analyzer reverse the above procedure Service Manual MEK 7222 4 17 4 DISASSEMBLY AND ASSEMBLY
157. the GAIN key to go to the SENS amp THRESHOLD screen to adjust gain Refer to the Adjusting Gain FS and FL for WBC 5 Part Differential Parameters in Rough Mode Using Polymer Microsphere Suspensions section FS Forward Small Size vertical axis of the scattergram FL Forward Large Complexity horizontal axis SD Side Granularity horizontal axis THR Threshold of FS When the CV is Not Optimum CV of FS or FL is over 7 0 If the polymer microsphere suspension is old it clots and CV value is not optimum Replace the polymer microsphere suspensions and perform the particle test again PARTICLE TEST 12 FEB G3 14 07 The polymer microsphere suspensions clots Service Manual MEK 7222 8 11 8 DEMONSTRATION GUIDE Adjusting the Flow Cell Unit Position 8 12 The scattergram is large If dust or bubbles accumulate inside the flow cell the scattergram of polymer microsphere suspensions becomes large Clean the flow cell by pressing the CLEAN FLOWCELL key on the OPERATIONS screen or clean the fluid path in the hematology analyzer by pressing the Clean key on the front panel If this does not improve it adjust the flow cell position PARTICLE TEST 12 FEB 03 14 07 FS HE SD CU 16 2 14 7 20 0 PEAK 6l The scattergram is large Check Points before Adjusting the Flow Cell Unit Position The flow cell unit position is adjusted to the most appropriate position at the factory Wh
158. the OPERATIONS screen to perform strong cleaning 2 Press the DRAIN BATHS key on the OPERATIONS screen to drain the measurement baths and sub baths 3 Press the Power key while holding down the Reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side 6 Remove the 2 filter joint assemblies by turning the tube connectors 6 8 Service Manual MEK 7222 6 MAINTENANCE 7 Remove the filter from each assembly Use tweezers to remove any dust from the filter If it is still dirty replace it with a new one 8 Reattach the filter joint assemblies NOTE When attaching the filter joint assembly be careful not to bend or damage the filter packing at the bottom of the measurement bath When there is a leakage check that there is no scratch or damage around the filter 9 Reattach the front cover and fasten it with the two screws on each side 10 On the OPERATION HISTORY screen press the RESET key for FILTERS to reset the counts to zero OPERATION HISTORY 26 RUG Gs 15 20 SS ee ee PA ae ee FILTERS 8 1000 counts ES 813000 counts GEM ME 100 onts GUN ox VERSION V02 01 51F25 Service Manual MEK 7222 6 9 6 MAINTENANCE Checking and Cleaning the Sub Baths Measurement
159. the flat cable from the printer unit as shown below 6 Completely remove the printer unit from the front cover To assemble the hematology analyzer reverse the above procedure Removing the Front Panel Unit 1 Press the main power switch on the rear panel to turn the power off The power lamp and main power lamp go off WARNING For your sefety before disassembling the hematolozy analyzer wait approx 10 minutes after turning off the main power switch 2 Open the front cover according to the procedure described in Opening the Front Cover 3 Remove the top cover according to the procedure described in Removing the Top Cover 4 Remove the 4 screws which secure the front panel unit to the chassis through the 2 hinges CAUTION Hold the front panel unit immediately after removing the 4 screws Otherwise the front panel unit can fall down and be damaged due to ts weight 4 20 Service Manual MEK 7222 Service Manual MEK 7222 4 DISASSEMBLY AND ASSEMBLY 5 Release the cable tie and disconnect the cable from the front panel unit as shown below Cable tie 6 Completely remove the front panel unit from the chassis To assemble the hematology analyzer reverse the above procedure 4 21 4 DISASSEMBLY AND ASSEMBLY Removing the Inlet Outlet Unit 1 Drain all the fluid from the hematology analyzer according to the procedure described in Draining the Hematolozy A
160. the operator s manual Automatic cleaning operation Check that the automatic cleaning operations are normal during this maintenance check Fluid leakage Check that there is no fluid leakage on the hematology analyzer especially after each automatic cleaning operation Dispense operation Check that the constant volume of the diluent is properly dispensed when pressing the Dispense Open key on the front panel in capillary blood mode Open Closed mode selection operation when the cap pierce unit is installed Check that the cap pierce unit properly works when selecting the Open mode or Closed mode Check that Pressing the Feed key on the front panel feeds the recording paper and there is no dot missing on the paper by pressing the Print key on the front panel 6 5 6 MAINTENANCE External Printer Unit Operation When the external printer is connected Bar Code Reader Operation When the bar code reader is installed Others 6 6 Check that the paper feed operation properly works on the external printer and there is no dot missing on the paper 1 Clean the light emitter and detector parts with a cotton swab moistened with 80 alcohol 2 Check that the bar code reading operation properly works Write down any other points on the check sheet Service Manual MEK 7222 6 MAINTENANCE Displaying Operation History Screen General You can display the total operating time total number of counts and num
161. ths 3 Press the power key while holding down the reset key to turn the power off Check that the power lamp is off 4 Remove the two screws on each side of the front cover of the hematology analyzer 5 Open the front cover by pulling it from the right side Service Manual MEK 7222 6 23 6 MAINTENANCE Sampling nozzle plate 6 Slide the sampling nozzle plate to the left 7 Remove the screw from the cover Pull the cover towards you and remove it from the cap pierce needle M eer 8 Pull the cap pierce needle upwards and remove it from the cap pierce rinse unit Turn the tube joint counterclockwise and disconnect it from the cap pierce needle Cap pierce needle Car pierce rinse unit 9 Replace the cap pierce needle with a new one 10 Assemble the cap pierce needle cover and put back the sampling nozzle plate to its original position 11 Reattach the front cover and fasten it with the two screws on each side 12 Turn the power on The hematology analyzer starts priming the fluid path OPERATION HISTORY 26 RUG 83 15 20 TOTAL COUNTS 1972 counts AAA A y eet 13 Display the OPERATION HISTORY scre
162. um gain Displays the PEAK LIST screen Change samples to Register the displayed sample on be displayed the peak list 3 Register an appropriate blood sample for adjustment on the peak list Register only the blood samples which can be used for adjustment You can register up to 12 samples You should have at least 5 samples registered Blood samples to be registered can be displayed with the or gt key Check points for blood samples which can be used for adjustment The distributions of NE LY and MO are elliptical shapes on the scattergram The distributions of NE LY and MO are separate from each other NE LY and MO should be located in the allotted positions Optimum scattergram MO a b a There is no NE distribution and MO and LY are not separated on the scattergram A scattergram of a leukemia patient b MO is distributed on NE A scattergram of a patient who has immature granulocytes c NE is distributed too close to MO The scattergram of a blood sample collected more than 8 hours ago 5 11 5 Adjustment 5 12 4 Press the ADD key to register the blood sample is displayed in the scattergram serial No after registration and the number of N in the MO PEAK table is incremented 5 After registering blood samples to the peak list check that the obtained result is optimum on the PEAK LIST screen Refer to the Editing the Peak List section 6 Press the AUTO k
163. utes to 8 hours after collection f Check the actual peak list for all samples to see if the gain is adjusted appropriately Make sure that the average peak of MO is optimum 4 Calibrate the hematology analyzer a Perform calibration measurement with MEK 3DN calibrator to obtain a mean value for calculating the new calibration coefficient The MEK 3DN calibrator can be used as a calibrator if it is stored at 2 to 8 C and is used within 3 days after opening To ensure higher accuracy use the calibrator soon after opening b Perform the calibration for CBC parameters WBC RBC HGB MCV PLT RDW MPV c Check that the calibration coefficients for WBC 5 part differential parameters LY MO EO BA are 1 000 on the CALIBRATION 8 1 8 DEMONSTRATION GUIDE screen In the gain adjustment performed in step 3 WBC 5 part differential parameters are already accurately calibrated Therefore you only need to check the calibration coefficient 5 Check the following data a Measure the MEK SDN H L hematology control and check that the obtained data falls within the acceptable range on the assay sheet attached to the hematology control b Count more than 10 venous blood samples of different healthy people which are 30 minutes to 8 hours after collection and make sure that there is no flag such as Left Shift Eosinophilia Blasts Immature Granulocyte or Atypical Lymphocytes displayed Required
164. works this LED lights D063 MV3 LED for operation check of MV3 valve When the MV3 works this LED lights D064 MV4 LED for operation check of MV4 valve When the MV4 works this LED lights D065 MV5 LED for operation check of MV5 valve When the MV5 works this LED lights D066 MV6 LED for operation check of MV6 valve When the MV6 works this LED lights D067 MV7 LED for operation check of MV7 valve When the MV7 works this LED lights D068 MV8 LED for operation check of MV8 valve When the MV8 works this LED lights D069 MV9 LED for operation check of MV9 valve When the MV9 works this LED lights Service Manual MEK 7222 7 3 7 Test Point Variable Resistor LED and Switch on Board 4WAY LIQUID SENSOR board VRO11 VR012 VRO13 VR014 TP011 LIQO Test point for output voltage from sensor for diluent and detergent TPO12 LIQ1 Test point for output voltage from sensor for hemolysing reagent HEMOLYNACS5 TPO13 LIQ2 Test point for output voltage from sensor for hemolysing reagent HEMOLYNAC3 TP014 LIQ3 Test point for output voltage from sensor in the sheath unit TPO15 5V 5 V DC terminal for digital circuit TPO16 ED Ground terminal for digital circuit VRO11 LIQO Variable resistor for adjustment of output voltage from sensor for diluent and detergent VROI2 LIQI Variable resistor for adjustment of output voltage from sensor for HEMOLYNACS reagent VRO13 LIQ2 Variable res
165. y 1 4 Service Manual MEK 7222 MCV MCH MCHC RDW PLT MPV 1 GENERAL Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually Excessive number of large PLT Excessive number of large PLT and or excessively high WBC may affect the MCV value Check by careful examination of the stained blood film MCH is determined from HGB and RBC values Therefore the limitations for HGB and RBC also affect MCH value MCHC is determined from HGB and HCT values Therefore the limitations for HGB and HCT also affect MCHC value RDW is determined from RBC value Therefore the limitations for RBC also affect RDW value Agglutinated RBC Agglutinated RBC may decrease RBC count and erroneous RDW This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Nutritional deficiency or blood transfusion Iron and or cobalamin and or folate deficiency may increase RDW Very small fragments Very small RBC RBC fragments and WBC fragments may be the cause in increased PLT count Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in decrease in PLT This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Very large PLT Large PLT may exceed the PLT threshold and may not be counted which results in low PLT count Chemotherapy Cytotoxic and immun
166. y connected NOTE e To handle the diluent container detergent container and waste container follow the instructions on each package To handle the Hemolynace3 or Hemolynace3N and Hemolynace5 hemolysing reagents follow the instructions in the operator s manual for each hemolysing reagent 3 Press the DRAIN ALL key on the OPERATIONS screen and press YES key The hematology analyzer starts draining all fluid Service Manual MEK 7222 8 DEMONSTRATION GUIDE Connect the spare tubes to the ISO3 HEMO3 or HEMO3N HEMOS CLN and CLN3 inlets and put the other ends of the tubes into the distilled water The optional YZ 0252 Cleaning bottle kit is available for easy setup Press the Clean key on the front panel to prime the hematology analyzer with distilled water Repeat steps 2 and 3 to drain all fluid from the hematology analyzer 7 Press the main power switch on the rear panel to turn the main power off Main power switch Service Manual MEK 7222 8 33 8 DEMONSTRATION GUIDE MEK 7222J K Hematology Analyzer Installation Check Sheet Use this check sheet to check that the hematology analyzer is properly installed The values in the gain adjustment and calibration should always be entered as they are useful in troubleshooting Facility Hospital Name Serial Number
167. ymer microsphere suspensions before adjusting gain in fine mode 1 Measure 10 human blood samples from different healthy persons which is 30 minutes to 8 hours after collection for all 22 parameters NOTE e Do not use a blood sample which was collected more than 8 hours before or refrigerated Some blood samples may cause poor hemolysing when you use a blood sample collected within 30 minutes In this case leave the blood sample more than 30 minutes before measurement Stir the blood sample carefully before measurement Be careful that too much stirring cause poor hemolysing Do not measure a blood sample which is clumped or clotted This may damage the hematology analyzer 2 Press the AUTO DIFF GAIN key on the SENS amp THRESHOLD screen SENS amp THRESHOLD 14 FEB 83 15 12 Service Manual MEK 7222 Service Manual MEK 7222 5 Adjustment The scattergram The number of data that serial number can be registered up to 50 samples The sample ID The date and time of AUTO DIFF GAIN 12 FEB O3 15 41 The mark appears when the sample is registered on measurement The number of th k list registered samples Ms ID 0000000001230 DATE 12 FEB B3 The target MO peaks TIME 15 40 MO PEAK N 1 AUE AIM The average peaks of the registered MO distribution MO peaks of the Actual gain displayed scattergram Gain set by the AUTO key optimum gain G oi gain to the LIST Qum optim
168. you 9 Put the new cap pierce unit to the original position and reverse the above procedure to assemble the hematology analyzer 4 36 Service Manual MEK 7222 Section 53 Adjustment Adjusting HGB Sensor Output Voltage oooconoccccnnocccnnoncccnnoncccnnancncnnann cnn conc nn nan nennen nnns 5 1 Adjusting Upper and Lower Sensor Output Voltage of the Manometer s 5 2 Compensating manometer Volume ssiri ennir enne enne nnne nnne nen 5 3 Adjusting Gain for WBC 5 Part Differential Measurement sssssseene 5 4 Counting the Polymer Microsphere Suspensions and Adjusting the Flow Cell POSitlOn iiia io air it 5 5 Adjusting Gain for WBC 5 Part Differential Measurement Roughly by Polymer Microsphere Suspensions ooconcocccincccccnonacacinnncnnonn carr nno nana nn nn cr rnnr rca 5 9 Adjusting Gain for WBC 5 Part Differential Measurement Finely by Human Blood Sample icono adri 5 10 Editing tlie Peak List sion A S 5 13 Manually Adjusting the Gain sssssssseeeeneeeneenmeneen nennen 5 15 Compensating Dilution Ratio tieniti tese eme ct en atten cde 5 15 Calibrating TOUCH Sc E EE 5 16 Adjusting Liquid Sensor Output Voltages ooonoccccnnoccccnnccnnnonnoncconanca nana ncnnannn cn rana n conan cc nnnnnn 5 17 Adjusting Gontrastot LCD Em 5 18 Service Manual MEK 7222 5C 1 5 Adjustment Adjusting HGB Sensor Output Voltage Service Manual MEK 7222 Open the front cover acco
169. ytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate BA count and BA Service Manual MEK 7222 8 31 8 DEMONSTRATION GUIDE Storing and Transporting the Hematology Analyzer WARNING This procedure must be performed to safely store and transport the hematology analyzer There are many expensive units installed in the hematology analyzer If there is any malfunction caused from not doing this procedure the expense necessary for performing maintenance for that damage will be charged to you When storing and transporting the hematology analyzer is necessary after demonstration clean it by the following procedures Clean the inside of the hematology analyzer only with distilled water After cleaning make sure that all distilled water is completely drained from the hematology analyzer If diluent is left inside the hematology analyzer the inside of the hematology analyzer will become dirty because of dried diluent crystal or other dust This increases background noise 1 Press the Clean key yy on the front panel to clean the fluid path Remove the diluent tube from the ISO3 inlet the hemolysing reagent tubes from the HEMO3 or HEMO3N and HEMOS inlets and the detergent tubes from the CLN3 and CLN inlets on the right side panel Do not disconnect the waste fluid tube from the WASTE outlet CAUTION Make sure that the waste fluid tube is correctl

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