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G1312-90006 - Agilent Technologies

1. Exchanging the Active Inlet Valve on page 82 78 1200 Series Binary Pump A User Manual Maintenance 7 3 Purge valve see Exchanging the Purge Valve Frit or the Purge Valve on page 88 4 Outlet ball valve see Exchanging the Outlet Ball Valve Sieve or the Complete Valve on page 86 5 Pump drive see Exchanging a Pump Drive in the Service Manual 6 Power supply see Exchanging the Power Supply in the Service Manual 7 HPM board see Exchanging the High Pressure Pump Main Board in the Service Manual 8 Fan see Exchanging the Fan in the Service Manual 9 Damper see Exchanging the Damper in the Service Manual 10 Solvent selection valve see Exchanging the Solvent Selection Valve on page 90 1200 Series Binary Pump A User Manual 79 7 Maintenance Simple Repairs The procedures described in this section can be done with the binary pump in place in the system stack Table9 Simple Repair Procedures Procedure Typical Condition Notes Checking and Cleaning the Solvent Filter on page 81 Exchanging the Active Inlet Valve on page 82 Exchanging the Outlet Ball Valve Sieve or the Complete Valve on page 86 Exchanging the Purge Valve Frit or the Purge Valve on page 88 Exchanging the Purge Valve Frit or the Purge Valve on page 88 Exchanging the Solvent Selection Valve on page 90 Exchanging the Pump Seals on page 94 Exchanging the Plungers o
2. 7 74 Maintenance Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD strap when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 14 Using the ESD Strap 1200 Series Binary Pump A User Manual Maintenance 7 Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 1200 Series Binary Pump A User Manual 75 7 Early Maintenance Feedback EMF 76 Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefi
3. corrugated waste tubing reorder pack Detector Figure 5 Recommended Stack Configuration Front View 1200 Series Binary Pump A User Manual 29 4 a ing the otacK LO 3 Installing the Pump For a detailed view of the flow connections refer to the section Flow connections in the product information of the individual modules Pressure output to recorder CAN Bus cable to handheld controller CAN Bus cable for inter module communication 5181 1516 0 5 m 5161 1519 1 0 m Analog signal to recorder Figure 6 30 Recommended Stack Configuration Rear View AC power GPIB or LAN to ChemStation AC power 1200 Series Binary Pump A User Manual Installing the Pump 3 Pressure output to recorder see Cable Overview in the Service Manual Analog signal to recorder see Cable Overview in the Service Manual GPIB or LAN to ChemStation see Cable Overview in the Service Manual If a single stack configuration becomes too high e g if an additional module like a G1327A ALS Thermostat is added or if your LC system is too high a two stack configuration may be a better setup Separate the stack between pump and autosampler and place the stack containing the pump on the right side of the stack containing the autosampler 1200 Series Binary Pump A User Manual 31 3 Installing the Pump Installing the Binary Pump Parts required Prepa
4. Figure 32 Outlet Ball Valve 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance 8 Purge Valve Assembly Table 15 Purge Valve Assembly Item Description Part Number Purge valve complete assembly G1311 60009 1 Valve body No part number 2 PTFE frit pack of 5 01018 22707 3 Gold seal 5001 3707 4 Cap pack of 4 5062 2485 Figure 33 Purge Valve Assembly 1200 Series Binary Pump A User Manual 119 Parts and Materials for Maintenance Active Inlet Valve Assembly 120 Table 16 Active Inlet Valve Assembly Item Description Part Number 1 Active inlet valve body No cartridge included G1312 60025 2 Valve cartridge 400 bar 5062 8562 Figure 34 Active Inlet Valve Assembly 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance 8 Accessory Kit G1311 68705 Table 17 Tools and Accessories Description Part Number Wrench 14 mm Seal insert too PTFE Frit pack of 5 Corrugated waste tube 1 2 m Corrugated waste tube reorder number 5 m Velocity regulator reorder number pack of 3 Hex key 4mm Wrench 1 4 5 16 inch Capillary pump to injection device length 900 mm ID 0 17 mm 8710 1924 01018 23702 01018 22707 no PN 5062 2463 5062 2486 8710 2392 8710 0510 G1329 87300 1200 Series Binary Pump A User Manual 121 8 Parts and Materials for Maintenance Seal Wash Option G1312 68711 Table 18 Active Seal Wash Option kit
5. damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the instrument has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection 1200 Series Binary Pump A User Manual Installing the Pump 3 Delivery Checklist Delivery Checklist Ensure all parts and materials have been delivered with the binary pump The delivery checklist is shown in Table 4 on page 27 In order to identify parts you may refer to Parts and Materials on page 110 Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 4 Binary Pump Checklist Description Quantity Binary pump 1 Solvent cabinet 1 5062 8591 Solvent bottle 2 or 4 9301 1450 amber bottle 9301 1420 transparent bottle Bottle head assembly 2 or 4 G1311 60003 Waste tube purge valve 1 5042 2461 reorder number 5 m Power cable 1 CAN cable 1 m 1 Remote cable As ordered Signal cable As ordered Service Manual 1 Accessory kit see Table 5 1 on page 28 1 if the binary pump is equipped with a solvent selection valve 1200 Series Binary Pump A User Manual 27 3 28 Installing the Pump Accessory Kit Contents G1311 68705 Table5 Accessory Kit Contents G1311 68705 Description Part Number Capillary pum
6. depending on the flow rate the drive motors are stopped and the active inlet valve is closed The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards Then the sequence starts again moving the pistons up and down between the two limits During the upward movement of the first piston the solvent in the pump head is pressed through the outlet ball valve into the second pumping unit The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system During the drawing stroke of the first piston the second piston delivers the drawn volume into the system Table 1 Pump Details Delay volume From mixing point to pump outlet dependent on back pressure 180 480 ul without mixer 600 900 pl with mixer Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active Inlet Valve SST sapphire ruby ceramic PTFE Outlet Valve SST gold sapphire ruby tantalum 1200 Series Binary Pump A User Manual Introduction 1 Table 1 Pump Details Adapter SST gold Purge Valve SST gold PTFE ceramic Damping Unit Gold SST For pump specifications see Site Requirements on page 18 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in th
7. descriptions of 14 electrostatic discharge ESD 73 106 EMF flag 76 EMF limits 77 EMF counters 76 environment 18 21 error condition 69 error messages 67 ESD electrostatic discharge strap 74 Exchanging the Active Inlet Valve Cartridge 84 exchanging active inlet valve 82 82 active inlet valve 80 84 interface board 106 internal parts 72 outlet ball valve sieve 86 outlet ball valve 86 outlet ball valve sieve 80 outlet ball valve 80 pistons 80 97 pump seals 94 pump seals 80 purge valve frit 88 purge valve 88 purge valve frit 80 purge valve 80 solvent selection valve 90 wash seals 98 wash seals 80 102 133 Index F fastest gradient response 61 features GLP 24 instrument layout 13 safety and maintenace 24 firmware download 132 updates 107 upgade downgrade 107 flow precision 23 23 flow range 23 flow connections 39 42 frequency range 22 frit 88 G gas solubility 50 gradient formation 23 hex key set 28 hexagonal key 3mm 94 97 104 hexagonal key 3mm 92 102 hexagonal key 4mm 94 97 104 hexagonal key 4mm 92 102 highest injection precision 58 high pressure mixing 6 hints for successful use 50 humidity 22 hydraulic system 23 indicator status 68 initialization 10 10 insert tool 102 installation pump module 32 installation 134 environment 21 instrument status indicator 69 lamp 69 interface board 106 interface cable 34 internet 132 introduction to the pump 6 L Lab
8. 1 introduction Electrical Connections 16 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 2 Site Requirements and Specifications Site Requirements 18 Power Consideration 18 Power Cords 19 Bench Space 20 Environment 21 Physical Specifications 22 Performance Specifications 23 gy Agilent Technologies 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the instrument Power Consideration The module power supply has wideranging capability see Table 2 on page 22 It accepts any line voltage in the range described in the above mentioned table Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cabl
9. Pump A User Manual NOTE Installing the Pump 3 Installing the Binary Pump The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch in the Service Manual 1200 Series Binary Pump A User Manual 35 3 Installing the Pump Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Agilent 1200 Series modules 1 Place the individual modules in a stack configuration as shown in Figure 5 on page 29 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 6 on page 30 5 Press in the power switches to turn on the modules 36 1200 Series Binary Pump A User Manual Installing the Pump 3 Connecting an Agilent 1200 Series Vacuum Degasser 1 Place the vacuum degasser in the stack of modules as shown in Figure 5 on page 29 2 Ensure the power switch on the front of the vacuum degasser is OFF switch stands out 3 Plug an APG cable into the APG
10. a Agilent 1200 Series System stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the outlet capillary binary pump to injection device to the outlet of the purge valve 1200 Series Binary Pump A User Manual Installing the Pump 3 Flow Connections of the Binary Pump with Solvent Selection Valve 8 Purge your system before first use see Priming and Purging the System on page 45 Bottle head assembly Solvent cabinet ll a Tube clip i O T WS MHS N S a gt semer Ct e Lae Channel A1 A H Way W Q Channel B1 O ap Ve Ai y OL Purge valve aaa Se int 4 Pump head Pump headchannel A channel B Active inlet Ss alee e Active inlet valve A Waste tubing A Waste outlet Outlet capillary to autosampler Channel A2 Channel B2 Solvent selection valve Figure 10 Binary Pump with Solvent Selection Valve 1200 Series Binary Pump A User Manual 41 3 Installing the Pump Flow Connections of the Binary Pump without Solvent Selection Valve Parts required Part number Description Other modules G1311 68705 Parts from accessory kit see Accessory Kit Contents in the Service Manual 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of
11. fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 1200 Series Binary Pump A User Manual 21 2 Site Requirements and Specifications Physical Specifications 22 Table2 Physical Specifications Type Weight Dimensions width x depth x height Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL Specification 15 5 kg 34 Ibs 180 x 345 x 435 mm 7 x 13 5 x 17 inches 100 240 VAC 10 50 or 60 Hz 5 220 VA 74 W 253 BTU 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6500 ft Up to 4600 m 14950 ft Installation Category II Pollution Degree 2 Comments Wide ranging capability Maximum Non condensing For storing the module For indoor use only Research Use Only Not for use in Diagnostic Procedures 1200 Series Binary Pump A User Manual Table 3 Site Requirements and Specifications 2 Performance Specifications Performance Specification Agilent 1200 Series Binary Pump Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compe
12. front cover Remove the top cover and foam 1 By using a screwdriver remove the metal plug in the z panel AZ Figure 23 Removing the metal plug from the z panel 2 Insert the socket delivered with the Seal Wash pump assembly into the hole on the z panel 3 Guide the wire of the active seal wash assembly through the hole and screw it onto the z panel 98 1200 Series Binary Pump A User Manual Maintenance 7 4 Guide the wire over the fan and plug the connector onto the mainboard connector P7 aie at 7 JA gt S Figure 24 Wire connected to the mainboard 5 Replace the foam and top cover 6 Disconnect all capillaries and tubes from the pump head and disconnect the active inlet valve cable 7 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws and remove the pump head from the pump drive 8 Place the pump head on the backside of the plungerhousing on a flat surface Loosen the lock screw two revolutions and while holding the lower half of the assembly carefully pull the pump head away from the plunger housing 9 Remove the support rings from the plunger housing and lift the housing away from the plungers 10 Install the support ring assembly from the active seal wash option kit into the plunger housing 1200 Series Binary Pump A User Manual 99 7 100 Maintenance Figure 25 Inserting the active seal wash support rings 11 Place the support rings
13. night and volatile solvent mixtures are used or e solvents have been changed For more information see the User Manual for the Agilent 1200 Series vacuum degasser 1200 Series Binary Pump A User Manual Optimizing Performance 5 When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and plungers in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 Molar or higher will be used for long time periods in the pump The seal wash option can be ordered by quoting part number 01018 68722 kit contains all parts needed for one pump head The active seal wash option kit can be ordered by quoting part number G1311 68711 The seal wash option comprises a support ring secondary seal gasket and seal keeper for both plunger sides A wash bottle filled with water isopropanol 90 10 should be placed above the pump in the solvent cabinet and gravity will maintain a flow through the pump head removing all possible buffer crystals from the back of the pump seal For the active seal wash a peristaltic pump is pumping the solvent through the pump head Running dry is the worst case for a seal and drastically reduces its lifetime The seal will build up sticky layers on the surface of the plunger These sticky layers will also reduce the lifet
14. on the plunger housing plungers not installed and snap the pump head and plunger housing together 12 Insert the plungers and carefully press them into the seal 13 Tighten the lock screw 14 Slide the pump head assembly onto the metering drive Apply a small amount of pump head grease part number 79846 65501 to the pumphead screws and the balls of the spindle drive Tighten the pumphead screws stepwise with increasing torque 15 Reconnect all capillaries tubes and the active inlet valve cable to its connector 16 Route the wash inlet tube into a bottle filled with a mixture of distilled water and isopropanol 90 10 and place the bottle above the pump in the solvent cabinet 17 Route the outlet of the wash tube into a waste container 1200 Series Binary Pump A User Manual Figure 26 Pumphead after completed installation 1200 Series Binary Pump A User Manual Maintenance Simple Repairs 101 7 Maintenance Exchanging the Wash Seals When If leaking Tools required hexagonal key3 mm 4 mm hexagonal key Insert tool Small flat head screwdriver Parts required Part number Description 1 0905 1175 Wash seal 6 5062 2484 Gasket seal wash pack of 6 Preparations Switch off binary pump at power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 92 1 Remove the seal keeper and the seal wash support rings from the plunger housing Remove the
15. points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting 1200 Series Binary Pump A User Manual 125 9 Appendix When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 126 1200 Series Binary Pump A User Manual Appendix 9 Safety Symbols Table 19 Safety Symbols Symbol Description The ap
16. premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings 1 Start channel A of the binary pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test see Pressure Test in the Service Manual 3 Your pump must be connected to a control software e g ChemStation EZChrom OL etc or handheld controller with which the pressure and ripple can be monitored otherwhise connect a signal cable between the pressure output of the isocratic pump and a recording device for example 339X integrator and set parameters e g Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device with the plot mode Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 1200 Series Binary Pump A User Manual Optimizing Performance 5 Table 7 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lsopropanol 100 Methan
17. seal keeper from the support ring assembly Seal keeper Ss Seal wash aR support ring Plunger housing Plunger Q 2 Using the blade of a flat head screwdriver remove seal wash gasket and the secondary seal from the support ring Support ring LX 102 1200 Series Binary Pump A User Manual Maintenance mple Repairs 7 Using the insert tool press the seal spring pointing upwards into the recess of the support ring Place a seal wash gasket in the recess of the support ring and replace the seal keeper Qa Insert tool ES Seal E Support ring gt gt 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 104 1200 Series Binary Pump A User Manual 103 7 Maintenance Reinstalling the Pump Head Assembly When Tools required Parts required 104 When reassembling the pump 3 mm hexagonal key 4 mm hexagonal key Description 79841 65501 PTFE lubricant 1 Slide the pump head assembly onto the pump drive Valve holder screw d Purge valve holder j Balls of spindle drive Pig Pumphead screws 2 Using a4 mm hexagonal key tighten the pumphead screws stepwise with increasing torque 3 Using a3 mm hexagonal key fix the purge valve holder to the pump head 1200 Series Binary Pump A User Manual Maintenance 7 Simple Repairs 4 Reconnect the capillaries tubing and the active inlet valve cable to the connecto
18. toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 11 Removing the Front Cover 2 Place the solvent cabinet on top of the binary pump 42 1200 Series Binary Pump A User Manual Installing the Pump 3 Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves Fix the tubes in the clips of solvent cabinet and binary pump Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the binary pump is not part of a Agilent 1200 Series System stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the outlet capillary binary pump to injection device to the outlet of the purge valve 1200 Series Binary Pump A User Manual 43 3 Installing the Pump Flow Connections of the Binary Pump without Solvent Selection Valve Bottle head assembly Solvent cabinet Tube clip Static Mixer Purge valve Pump he
19. valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pumping system 1200 Series Binary Pump A User Manual 7 1 introduction Overview of the Binary Pump Purge valve Pump outlet lt To waste Outlet valve Inlet i Inlet valve P From solvent bottle From solvent bottle Piston Pump head A Pump head B Figure2 The Hydraulic Path A seal wash optional is available when the pump is used with buffer solutions 8 1200 Series Binary Pump A User Manual Introduction 1 Overview of the Binary Pump How Does the Binary Pump Work The liquid runs from the solvent reservoir through an active inlet valve Each side of the binary pump comprises two substantially identical pump units Both pump units comprise a ball screw drive and a pump head with two sapphire pistons for reciprocating movement gt To mixing chamber From solvent bottle Seal Piston Ball screw drive Pee Gear Motor with encoder Figure 3 Principle of Pump Unit A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at double the speed of the second piston The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inn
20. will provide also the latest firmware of the Agilent 1200 Series modules for download 132 1200 Series Binary Pump A User Manual Index Index A accessory kit 28 active inlet valve 82 82 120 active inlet valve 80 84 active seal wash 6 59 adapter 43 82 84 Agilent on internet 132 Agilent Diagnostic Software 66 Lab Advisor Software 66 algae growth 53 algae 50 alternative seal material 60 ambient operating temperature 22 ambient non operating temperature 22 analog output 23 AUTO mode 12 AUX output 37 ball screw drive 9 battery safety information 129 BCD board 106 bench space 20 binary pump checklist 27 bottle head assembly 27 buffer application 50 buffer solutions 6 C cable CAN 27 interface 34 power 27 remote 27 signal 27 capillary pump to injection device 28 changing solvents 46 checklist binary pump 27 cleaning 75 column 6 composition precision 23 23 composition range 23 compressibility compensation 23 compressibility compensation 11 62 condensation 21 connections flow 39 42 control software 38 counter seal wear 77 counter EMF 76 D damaged packaging 26 damaged parts 27 damping unit 10 degreaser spray 97 degreaser spray 102 delay volume 61 delay volume 10 29 delivery checklist 27 27 design 7 Diagnostic software 66 dimensions 22 dual piston in series design 7 1200 Series Binary Pump A User Manual E early maintenance feedback EMF 13 electrical connections
21. 1 4 inch 94 tweezers 88 U unpacking the pump 26 V vacuum degasser operational hints 58 vacuum degasser when to use 58 vacuum degasser 6 24 50 variable reluctance motor 9 variable stroke volume 12 velocity regulator 28 voltage range 22 wW wall socket 19 wash seals 80 102 waste tube 28 weight 22 wrench 1 4inch 86 94 wrench 1 4inch 88 wrench 14mm 82 84 86 wrench 14mm 88 wrench 1 4inch 92 wrench 1 4 5 16inch 28 wrench 14mm 28 135 www agilent com In This Book This manual contains technical reference information about the Agilent 1200 Series Binary Pump The manual describes the following e introduction e requirements e installation e configuring the pump e using the pump e optimizing performance e troubleshooting and diagnostics e maintenance e parts and materials e identifying cables e safety Agilent Technologies 2008 Printed in Germany 11 08 G1312 90006 fe Agilent Technologies
22. Advisor software 66 aboratory bench 20 amp power supply 68 amp status 68 amp instrument status 69 eak test 67 ine frequency 22 ine voltage 22 iquimeter 95 ithium batteries 129 M main assemblies overview 78 maintenance procedures 76 maintenance procedures 12 maintenance replacing firmware 107 minimum flow rate 50 missing parts 27 mixer 7 non operating altitude 22 non operating temperature 22 not ready condition 69 0 operating Altitude 22 operating temperature 22 optimum performance 29 outlet ball valve 86 118 outlet ball valve 80 overview pump 7 P parts active inlet valve 120 damaged 2 missing 27 outlet ball valve 118 pump head assembly with seal wash 116 pumphead 114 pump housing and main assemblies 113 pump housing and main assemblies 111 performance specification 23 pHrange 23 physical specifications 22 piston 9 80 97 power supply indicator 68 power supply lamp 68 power consideration 18 power consumption 22 power input socket 19 precision 50 prerun condition 69 pressure pulsation 23 pressure sensor readings 37 pressure pulsation 12 62 pressure range 60 pressure test 67 pressure operating range 23 1200 Series Binary Pump A User Manual Index priming withapump 45 48 with a syringe 45 with a pump 58 with a syringe 58 PTFE frit 88 PTFE frit 28 80 pump head assembly 114 pump head assembly with seal wash 116 pump seals 94 pump seals 50 80 pur
23. Agilent 1200 Series Binary Pump User Manual ot Agilent Technologies Notices Agilent Technologies Inc 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61312 90006 Edition 11 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep ar
24. F the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary at the purge valve and the connecting tube from solvent selection valve if installed to the AIV 7 Rinse your system with the solvent used for your next application 96 1200 Series Binary Pump A User Manual Exchanging the Plungers When When scratched Tools required hexagonal key3 mm 4 mm hexagonal key Maintenance 7 Parts required Part number Description 1 5063 6586 Plunger Preparations Switch off binary pump at power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 92 1 Check the plunger surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched Plunger surface 2 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 104 1200 Series Binary Pump A User Manual 97 7 Maintenance Installing the Active Seal Wash Option When When installing seal wash option Tools required 4 mm hexagonal key Screwdriver Pozidrive 1 Parts required Part number Description 1 G1311 68711 Active Seal Wash Option kit Isocratic or Quaternary pump 1 G1312 68711 Active Seal Wash Option kit Binary pump Preparations Switch off the pump at the main power switch Remove the
25. Repair 78 Simple Repairs 80 Checking and Cleaning the Solvent Filter 81 Exchanging the Active Inlet Valve 82 Exchanging the Active Inlet Valve Cartridge 84 Exchanging the Outlet Ball Valve Sieve or the Complete Valve 86 Exchanging the Purge Valve Frit or the Purge Valve 88 Exchanging the Solvent Selection Valve 90 Removing the Pump Head Assembly 92 Exchanging the Pump Seals 94 Exchanging the Plungers 97 Installing the Active Seal Wash Option 98 Exchanging the Wash Seals 102 Reinstalling the Pump Head Assembly 104 Exchanging the Optional Interface Board 106 Replacing the Module s Firmware 107 ot Agilent Technologies n 7 Maintenance Introduction to Maintenance and Repair Simple Repairs The module is designed for easy repair The most frequent repairs such as plunger seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Simple Repairs on page 80 Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the module from the stack removing the covers and disassembling the module The security lever at the power input socket prevents that the module cover is taken off when line power is still connected 72 1200 Series Binary Pump A User Manual Maintenance 7 Warnings and Cautions Module is partially energized when switched off as long as the
26. ad channel A Adapter Active inlet valve A Waste tubing 8 Purge your system before first use see Priming and Purging the System on page 45 py Outlet capillary to autosampler Figure 12 Pump head channel B Active inlet valve B Waste outlet Flow Connection of Binary Pump Without Solvent Selection Valve 1200 Series Binary Pump A User Manual Installing the Pump 3 Priming and Purging the System If a degasser is installed it can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the vacuum degasser or system with a syringe is recommended when e vacuum degasser or system is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the tubes Priming the system by using the pump at high flow rate 8 5 ml min is recommended when e pumping system was turned off for a length of time for example overnight and if volatile solvent mixtures are used or e solvents have been changed 1200 Series Binary Pump A User Manual 45 3 Installing the Pump Priming with a Syringe WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as d
27. and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series Binary Pump A User Manual Contents Contents 1 Introduction 5 Introduction to the Binary Pump 6 Overview of the Binary Pump 7 Instrument Layout 13 Electrical Connections 14 2 Site Requirements and Specifications 17 Site Requirements 18 Physical Specifications 22 Performance Specifications 23 3 Installing the Pump 25 Unpacking the Binary Pump 26 Optimizing the Stack Configuration 29 Installing the Binary Pump 32 Connecting Modules and Control Software 36 Flow Connections of the Binary Pump with Solvent Selection Valve 39 Flow Connections of the Binary Pump without Solvent Selection Valve 42 Priming and Purging the System 45 4 Using the Binary Pump 49 Hints for Successful Use of the Binary Pump 50 Solvent Information 52 Prevent Blocking of Solvent Filters 53 Algae Growth in HPLC Systems 54 5 Optimizing Performance 57 When to Use a Vacuum Degasser 58 When to use the Seal Wash Option 59 When to Use Alternative Seals 60 When to Remove the Static Mixer 61 How to Optimize the Compressibility Compensation Setting 62 1200 Series Binary Pump A User Manual Contents 6 Troubleshootin
28. anical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1200 Series Binary Pump A User Manual 13 1 Introduction Electrical Connections 14 The GPIB connector is used to connect the module with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your module The switches are preset to a default address and is recognized once after power is switched ON The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1200 Series module data transfer and synchronization One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number out
29. ary Pump A User Manual 19 2 Site Requirements and Specifications CAUTION Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 20 Bench Space The module dimensions and weight see Table 2 on page 22 allow to place the module on almost any laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1200 Series system make sure that the bench is designed to carry the weight of all the modules The module should be operated in a horizontal position 1200 Series Binary Pump A User Manual Site Requirements and Specifications 2 Environment Your pump will work within the specifications at ambient temperature and relative humidity described in Table 2 on page 22 The pump is designed to operate in a typical electromagnetic environment EN61326 1 where FF transmitters such as mobile phones should not be used in close proximity CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature
30. ate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood
31. bles other than the ones supplied by Aligent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Radio interference at unscreened cables If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 130 1200 Series Binary Pump A User Manual Appendix 9 Sound Emission Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1200 Series Binary Pump A User Manual 131 9 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products amp Services Life Sciences amp Chemical Analysis Solutions It
32. conditions that will decrease the gas solubility over time for example warming up the solvents For highest precision and reproducibility use a vacuum degasser When using the binary pump with vacuum degasser before operating the binary pump flush the degasser with at least two degasser tubing volumes 80 ml especially when the pumping system was turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels see Priming and Purging the System on page 45 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 53 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 ml min with an open purge valve When using the binary pump at low flow rates below 0 2 ml min check all 1 16 inch fittings for any signs of leaks Whenever possible use a minimum flow rate of 5 ul min per solvent channel to avoid crossflow of solvent into the unused pump channel Whenever exchanging the pump seals the purge valve frit and the outlet ball valve sieve should be exchanged too When using buffer solutions flush the system with water before switching it off The seal wash option shoul
33. d be used when buffer solutions of 0 1 molar or higher will be used for long time periods Check the pump plungers for scratches when changing the piston seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal 1200 Series Binary Pump A User Manual Using the Binary Pump 4 e After changing the plunger seals apply the seal wear in procedure see Exchanging the Pump Seals on page 94 e Place the aqueous solvent on channel A and the organic solvent on channel B The default compressibility settings are set accordingly 1200 Series Binary Pump A User Manual 51 4 Using the Binary Pump Solvent Information 52 Always filter solvents through 0 4 um filters small particles can permanently block the capillaries and valves Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric and nitric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt 2COCly 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabil
34. dia preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Symptoms Observed with the Agilent 1200 Series HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1200 Series where helium is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1200 Series can cause the following to occur e PTFE frits part number 01018 22707 purge valve assembly and column filter blockage causing increased system pressure Algae appear as white or yell
35. dicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition 1200 Series Binary Pump A User Manual 69 6 Troubleshooting and Diagnostics User Interfaces 70 Depending on the User Interface the available test vary Some descriptions are only available in the Service Manual Table 8 Test Functions available vs User Interface Test ChemStation Instant Pilot Control Module Agilent LC G4208A G1323B Diagnostic Software Pressure Test Yes No Yes Leak Test Yes No Yes 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 7 Maintenance Introduction to Maintenance and Repair 72 Simple Repairs 72 Exchanging Internal Parts 72 Warnings and Cautions 73 Using the ESD Strap 74 Cleaning the module 75 Early Maintenance Feedback EMF 76 EMF Counters 76 Using the EMF Counters 77 Overview of Maintenance and
36. e 400 bar Switch off binary pump at power switch 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector 3 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents BPs without the solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV 4 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head 5 Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 6 Position the valve so that the solvent inlet tube connection points towards the front 1200 Series Binary Pump A User Manual Maintenance 7 7 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve Solvent inlet tube Active inlet valve Figure 16 Exchanging the Active Inlet Valve 8 Reconnect the Active Inlet Valve cable to the connector at the Z panel and the inlet tube to the valve 9 Reinstall the front cover After an exchange of the valve it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly 1200 Series Binary Pump A User Manual 83 7 Maintenance Exchanging the Active Inl
37. e adapter at the AIV Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Cleaning the Solvent Filter 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour 2 Thoroughly flush the filter with bidistilled water remove all nitric acid some capillary columns can be damaged by nitric acid 3 Replace the filter 1200 Series Binary Pump A User Manual 81 7 Maintenance Exchanging the Active Inlet Valve When Tools required Parts required Preparations 82 If internally leaking backflow Wrench 14 mm Part number Description 1 G1312 60025 Active inlet valve body 1 5062 8562 Valve cartridg
38. e solvent selection valves 2 Using a Pozidriv screwdriver 1 loosen the holding screws of the valves Pull the valve module out of its connector 4 Hold the two plastic bodies of the valves and pull the two solvent selection valves apart 5 Exchange the defective solvent selection valve Press the exchanged valve new half together with the properly working old half 6 Connect the valve module to its electrical connectors and fix the assembly with the two holding screws 90 1200 Series Binary Pump A User Manual Maintenance Simple Repairs 7 Reinstall solvent tubes and the active inlet valve connection tubes Solvent tubes Solvent selection valve Connecting tubes Figure 22 Exchanging the solvent selection valve 1200 Series Binary Pump A User Manual 7 91 7 Maintenance Removing the Pump Head Assembly When Tools required Preparations CAUTION Exchanging pump seals Exchanging plungers Exchanging seals of the seal wash option Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Switch off pump at the main power switch and unplug the power cable Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 92 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve and mixing junction The following procedure describes the rem
39. e system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previous described loss of volume during the delivery stroke of the first piston 1200 Series Binary Pump A User Manual 11 1 introduction How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each plunger stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes will generate pressure pulsations of smaller amplitude than higher stroke v
40. e to the Instrument while the covers are removed 18 1200 Series Binary Pump A User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of each of the power cords is identical It plugs into the power input socket at the rear of the module The male end of each of the power cords is different and designed to match the wall socket of a particular country or region Electric Shock The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables The use of cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series Bin
41. er diameter of the pump head chamber allowing the solvent to fill the gap in between The first piston has a stroke volume in the range of 20 ul to 100 ul depending on the flow rate The microprocessor controls all flow rates 1200 Series Binary Pump A User Manual 9 1 10 Introduction in a range of 1 ul min to 5 ml min The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first pump unit The outlet of the pump unit is connected directly to the second pump unit The outlet of the second pump unit is connected via a small mixing chamber a coil and the damping unit to the purge valve assembly The outlet of the purge valve assembly is then connected to the following chromatographic system When turned on the pump runs through an initialization procedure to determine the upper dead center of the first piston of both pump channels The first piston moves slowly upwards to the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters for the two pump channels The active inlet valve is opened and the down moving piston draws solvent into the first pump head At the same time the second piston is moving upwards delivering into the system After a controller defined stroke length
42. er settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When performance indicates maintenance is necessary take note of the values displayed by pump liquimeter and seal wear counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1200 Series Binary Pump A User Manual 77 7 Maintenance Overview of Maintenance and Repair Overview of Maintenance and Repair Figure 15 on page 78 shows the main assemblies of the binary pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require to remove the module from its stack and to open the top cover Figure 15 Overview of Repair Procedures 1 Leak sensor see Exchanging the Leak Sensor in the Service Manual 2 Active inlet valve see
43. escribed in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 46 Before using a new degasser or new tubings for the first time 1 Prime all tubings with at least 30 ml of iso propanol no matter whether the channels will be used with organic mobile phase or with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows 2 Replace the current solvent with adequate organic solvent see Table 6 on page 48 if current solvent is organic or with water if current solvent is an inorganic buffer or contains salt Disconnect solvent tube from your pump Connect syringe adapter to solvent tube Push syringe adapter onto syringe ao 1 A ww Slowly pull the syringe plunger to draw at least 30 ml of solvent through degasser and tubing Replace the priming solvent with the new solvent of your choice 8 Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing 9 Disconnect syringe adapter from solvent tube 10 Connect solvent tube to your pump 11 Repeat step 3 on page 46 through step 10 on page 46 for the other channel s of the binary pump 1200 Series Binary Pump A User Manual Installing the Pump 3 When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore
44. et Valve Cartridge When Tools required Parts required Preparations 84 If internally leaking backflow Wrench 14 mm Part number Description 1 G1312 60025 Active inlet valve body 1 5062 8562 Valve cartridge 400 bar Switch off pump at the main power switch and unplug the power cable 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector 3 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents BPs without the solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV 4 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head 5 Using a pair of tweezers remove the valve cartridge from the actuator assembly 6 Clean the area in the actuator assembly Flush the cartridge area thouroughly 7 Insert a new valve cartridge into the actuator assembly make sure the valve cartridge is fully inserted into the actuator assembly 8 Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 9 Position the valve so that the solvent inlet tube connection points towards the front 10 Using the 14 mm wrench tighten the nut by turning the valve in its final position do not overtighten the valve 1200 Series Binary Pump A User Manual Maintenance 7 Simple Repai
45. for Binary pump Description Part Number Seal wash pump assembly includes pump casette and pump motor 5065 9953 Pump casette Silicone 5042 8507 Support ring seal wash 4 EA for Binary pump 5062 2465 Secondary seal pre installed in support rings 0905 1175 Gasket wash seal 4 EA for binary pump for re order pack of 6 5062 2484 Seal keeper 4 EA for binary pump 5001 3743 Silicone rubber tubing 1mm I D 3 m 0890 1764 Seal 2 packs of 2 for binary pump 5063 6589 Seals insert tool 01018 2370 122 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 9 Appendix General Safety Information 124 General 124 Operation 125 Safety Symbols 127 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 128 Lithium Batteries Information 129 Radio Interference 130 Radio interference at unscreened cables 130 Sound Emission 131 Sound Emission 131 Agilent Technologies on Internet 132 ee Agilent Technologies 123 9 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ens
46. g and Diagnostics 65 Agilent Lab Advisor Software 66 Overview of the Pump s Indicators and Test Functions 67 Status Indicators 68 User Interfaces 70 Maintenance 71 Introduction to Maintenance and Repair 72 Early Maintenance Feedback EMF 76 Overview of Maintenance and Repair 78 Simple Repairs 80 Parts and Materials for Maintenance 109 Parts and Materials 110 Pump Head Assembly 114 Pump Head Assembly with Seal Wash 116 Outlet Ball Valve Assembly 118 Purge Valve Assembly 119 Active Inlet Valve Assembly 120 Accessory Kit G1311 68705 121 Seal Wash Option G1312 68711 122 Appendix 123 General Safety Information 124 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 128 Lithium Batteries Information 129 Radio Interference 130 Sound Emission 131 Agilent Technologies on Internet 132 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 1 Introduction Introduction to the Binary Pump 6 Overview of the Binary Pump 7 How Does the Binary Pump Work 9 How Does Compressibility Compensation Work 11 How Does Variable Stroke Volume Work 12 Early Maintenance Feedback EMF 12 Instrument Layout 13 Electrical Connections 14 ee Agilent Technologies 1 Introduction Introduction to the Binary Pump Introduction to the Binary Pump The binary pump comprises two identical pumps integrated into one housing It provides gradient generation byhigh pressure mixing Dega
47. g the Pump 3 Flow Connections of the Binary Pump with Solvent Selection Valve Parts required Part number Description Other modules G1311 68705 Parts from accessory kit see Accessory Kit Contents in the Service Manual 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 9 Removing the Front Cover 2 Place the solvent cabinet on top of the binary pump 1200 Series Binary Pump A User Manual 39 3 40 Installing the Pump Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Connect the solvent tubes from the bottle head assemblies to the inlet connectors Al A2 B1 and B2 of the solvent selection valve and label the tubes accordingly Fix the tubes in the clips of solvent cabinet and binary pump Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the binary pump is not part of
48. ge valve 88 purge valve 50 80 purge valve assembly 10 p urging the pump 45 reassembling the pump head 104 recommended pH range 23 removing the pump head 92 repair procedures 80 repairs replacing firmware 107 using the ESD strap 74 reproducibility 50 runmode 69 S safety class 124 safety information lithium batteries 129 safety general information 124 standards 22 symbols 127 sapphire piston 9 screwdriver pozidriv 1 90 94 97 screwdriver pozidriv 1 102 screwdriver flat head 102 seal insert tool 28 sealwash 59 seal wear counters 77 sealwash 8 when to use 59 seal wear counter 95 seal alternative material 60 seals 50 80 97 98 98 102 security lever 33 security lever 72 setable flow range 23 shipping containers 26 sieve 80 86 118 simple repair procedures 80 simple repairs 72 site requirements 18 snap fastener 39 42 solvent cabinet 40 43 solvent filters checking 81 cleaning 81 solvent inlet filter 50 solvent selection valve 6 39 solvent bottle 27 solvent cabinet 27 50 solvent filters prevent blocking 53 solvent information 52 sonic bath 86 sovent selection valve 90 specification performance 23 physical 22 stack configuration rear view 30 stack configuration front view 29 stack configuration 29 static mixer 61 1200 Series Binary Pump A User Manual status indicators 68 status indicator 67 stroke volume 9 12 syringe adapter 46 T tools screwdriver pozidriv 1 94 wrench
49. he tests and diagnostic features as provided by the Agilent Lab Advisor Software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1200 Series Binary Pump A User Manual Troubleshooting and Diagnostics 6 Overview of the Pump s Indicators and Test Functions Status Indicators The pump is provided with two status indicators which indicate the operational state prerun run and error states of the pump The status indicators provide a quick visual check of the operation of the pump see Status Indicators on page 68 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagnostic Software Pressure Test The pressure test is a quick test designed to determine the pressure tightness of the system After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight up to 400 bar see Service Manual Leak Test The leak test is a diagnostic test designed to determine the pressure tightness of the pump When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance The follow
50. hold waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1200 Series Binary Pump A User Manual Appendix 9 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1200 Series Binary Pump A User Manual 129 9 Appendix Radio Interference Never use ca
51. ht be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Lab Monitor amp Diagnostic Software LMD Instant Pilot G4208A only if supported by module Control Module G1323B only if supported by module Description Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware the following steps have to be performed 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load the firmware into the module as described in the documentation The G1312B Binary SL requires firmware revision A 06 02 or above main and resident Due to a different hardware platform there is no way to convert a G1314D VWD and the G1314E VWD SL Plus to a G1314A B VWD or G1314C VWD SL 1200 Series Binary Pump A User Manual 107 7 Maintenance Simple Repairs 108 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 8 Parts and Materials for Maintenance Parts and Materials 110 Pump Head Assembly 114 Pump Head Assembly with Seal Wash 116 Outlet Ball Valve Assembly 118 Purge Valve Assembly 119 Active I
52. ime of the primary seal Therefore the tubes of the wash option should always be filled with solvent to prolong the lifetime of the wash seal Always use a mixture of bidistilled water 90 and isopropanol 10 as wash solvent This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water For information on the installation of the active seal wash option refer to Installing the Active Seal Wash Option on page 98 1200 Series Binary Pump A User Manual 59 5 Optimizing Performance When to Use Alternative Seals 60 The standard seal for the pump can be used for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend the use of the polyethylene seals part number 0905 1420 pack of 2 These seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range 0 200 bar When used above 200 bar their lifetime will be significantly reduced DO NOT apply the seal wear in procedure performed with new standard seals at 400 bar 1200 Series Binary Pump A User Manual Optimizing Performance 5 When to Remove the Static Mixer The binary pump is equipped with a static mixer The total delay volume of the pump is 600 900 ul The
53. ing sections describe these functions in detail see Service Manual 1200 Series Binary Pump A User Manual 67 6 Troubleshooting and Diagnostics Status Indicators Two status indicators are located on the front of the binary pump The lower left one indicates the power supply status the upper right one indicates the instrument status ka a ower supply indicator Figure 13 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON When the indicator is off the module is turned OFF Otherwhise check power connections availability of power or check functioning of the power supply 68 1200 Series Binary Pump A User Manual Troubleshooting and Diagnostics 6 Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions When the status indicator is OFF and power switch light is ON the module is in aprerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a setpoint or while a self test procedure is running An error condition is in
54. izing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1200 Series Binary Pump A User Manual Using the Binary Pump 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 1200 Series Binary Pump A User Manual 53 4 Using the Binary Pump Algae Growth in HPLC Systems 54 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous me
55. mixer has a volume of 420 ul The static mixer and both connecting capillaries can be replaced by a small capillary G1312 67301 under the following conditions e the delay volume of the pump should be reduced to a minimum for fastest gradient response and e the detector is used at medium or low sensitivity Removing the mixer will result in an increase of the composition ripple and higher detector noise 1200 Series Binary Pump A User Manual 61 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 62 The compressibility compensation default settings are 50 x 10 6 bar best for most aqueous solutions for pump head A and 115 x 10 bar to suit organic solvents for pump head B The settings represent average values for aqueous solvents A side and organic solvents B side Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side Under normal conditions the default settings reduce the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications If the compressibility values for the solvents used differ from the default settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimized by using the values for various solvents described in Table 7 on page 63 If the solvent in use is not listed in the compressibility table when using
56. n page 97 Exchanging the Wash Seals on page 102 Exchanging the Optional Interface Board on page 106 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If internally leaking If pump performance indicates seal wear If scratched When seals show indication of leaks If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 ml min H 0 with purge valve open indicates blockage Error messages Valve failed or Valve Fuse are generated Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals Leaks at lower pump head side loss of wash solvent Error condition indicated by red status indicator 80 1200 Series Binary Pump A User Manual Maintenance 7 Checking and Cleaning the Solvent Filter When Parts required Preparations CAUTION If solvent filter is blocked Description Concentrated nitric acid 35 Bidistilled water 1 Beaker Remove solvent inlet tube from th
57. ned time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The pump provides a series of EMF counters for the pump head Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The pump provides the following EMF counters e liquimeter pump A e seal wear pump A e liquimeter pump B e seal wear pump B Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset of the counters Both liquimeters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 1200 Series Binary Pump A User Manual Maintenance 7 Seal Wear Counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Using the EMF Counters The us
58. nger Plunger housing including springs Support ring seal wash Secondary seal Wash tube 1 0 m Gasket seal wash pack of 6 Seal keeper pack of 2 Seal pack of 2 or Seal pack of 2 for normal phase applications optional Capillary outlet valve to piston 2 Pump chamber housing Active inlet valve body Replacement cartridge for active inlet valve 400 bar Screw purge valve holder Purge valve holder Outlet ball valve Screw lock Apdater Purge valve assembly Screw M5 60 mm lg Seal wash pump assembly G1311 60005 5063 6586 G1311 60002 5062 2465 0905 1175 0890 1764 5062 2484 5001 3743 5063 6589 0905 1420 G1312 67300 G1311 25200 G1312 60025 5062 8562 0515 0175 G1312 23200 G1312 60012 5042 1303 G1312 23201 G1311 60009 0515 2118 5065 9953 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance 8 Pump Head Assembly with Seal Wash lA A Es S 5 L On a FAA A 19 Figure 31 Pump Head Assembly with Seal Wash Option 1200 Series Binary Pump A User Manual 117 Parts and Materials for Maintenance Outlet Ball Valve Assembly 118 Table 14 Outlet Ball Valve Assembly Item Description Part Number Outlet ball valve complete assembly G1312 60012 1 Socket cap G1312 21208 Housing screw 01018 22410 3 Outlet valve cartridge No part number 4 Sieve pack of 10 5063 6505 5 Gold seal outlet 5001 3707 6 Cap pack of 4 reoder number 5062 2485
59. nlet Valve Assembly 120 Accessory Kit G1311 68705 121 Seal Wash Option G1312 68711 122 gy Agilent Technologies 109 8 Parts and Materials for Maintenance Parts and Materials Parts and Materials Figure 28 Overview of Main Assemblies Front View 110 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance 8 Table 10 Repair Parts Pump Housing and Main Assemblies Front View Item Description Part Number 1 Pump head see Pump Head Assembly on page 114 G1311 60004 2 Pump drive assembly G1311 60001 Exchange assembly pump drive G1311 69001 3 Cable assembly AIV to main board G1311 61601 4 High pressure pump main board HPM G1312 66520 Exchange assembly HPM board G1312 69520 5 Cable assembly solvent selection valve G1312 61602 6 Fan assembly 3160 1017 7 Damping unit 79835 60005 8 Solvent selection valve half of a complete valve G1312 60000 Screw solvent selection valve 5022 2112 9 Leak pan pump 5042 8590 1200 Series Binary Pump A User Manual 111 8 Parts and Materials for Maintenance Parts and Materials Figure 29 Overview of Main Assemblies Rear View 112 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance Table 11 Repair rear panel and connectors Item Description Part Number 1 Nut M14 analog output 2940 0256 2 Washer analog output 2190 0699 3 Standoff remote connector 1251 7788 4 Standoff GPIB connec
60. not be fully degassed Pump for approximately 10 minutes with your selected flow rate before starting any application This will allow the vacuum degasser to properly degas the solvent in the degasser tubes The pump should never be used for priming empty tubings never let the pump run dry Use the syringe to draw enough solvent for completely filling the tubings to the pump inlet before continueing to prime with the pump 1200 Series Binary Pump A User Manual 47 3 48 Installing the Pump Priming with the Pump When the pumping system has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Therefore priming of the vacuum degasser and the pumping system is required before starting an application 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min Flush the vacuum degasser and all tubes with at least 30 ml of solvent Set flow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your application oa Ff amp w N Repeat step 1 on page 48 through step 4 on page 48 for the other channel s of the binary pump 6 Close the purge valve and set the required composition and flow rate for your ap
61. nsation Recommended pH range Gradient formation Composition range Composition precision Composition accuracy Control and data evaluation Analog output Communications Specification Two dual piston in series pumps with proprietary servo controlled variable stroke drive floating piston design and active inlet valve Setpoints 0 001 5 ml min in 0 001 ml min increments 0 1 5 0 ml min 0 07 RSD or 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 l min whatever is greater Operating range 0 400 bar 0 5880 psi up to 5 ml min lt 2 amplitude typically lt 1 at 1 ml min isopropanol at all pressures gt 1 MPa User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel High pressure binary mixing delay volume 180 480 ul without mixer 600 900 pl with mixer dependent on back pressure 1 99 or 5 l min per channel whatever is greater 0 5 absolute 0 15 RSD at 1 ml min Agilent ChemStation for LC For pressure monitoring 2 mV bar one output Controller area network CAN GPIB RS 232C APG Remote ready start stop and shut down signals LAN optional 1200 Series Binary Pump A User Manual 23 2 Site Requirements and Specifications Table3 Performance Specification Agilent 1200 Series Binary Pump Safety and maintenance Exten
62. ol 120 1 Propanol 100 Toluene 87 Water 46 6 Repeat step 1 on page 62 through step 5 on page 62 for the B channel of your binary pump 1200 Series Binary Pump A User Manual 63 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 64 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 66 Overview of the Pump s Indicators and Test Functions 67 Status Indicators 68 Power Supply Indicator 68 Instrument Status Indicator 69 User Interfaces 70 oe Agilent Technologies 65 Troubleshooting and Diagnostics Agilent Lab Advisor Software 66 The Agilent Lab Advisor Software is a standalone product that can be used with or without data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series HPLC modules This includes tests and calibrations procedures as well as the different injector steps to perform all the maintenance routines Agilent Lab Advisor also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument T
63. olumes at same flow rates In addition the frequency of the pressure pulsations will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes resulting in less flow ripple will improve composition ripple The module uses a processor controlled spindle system to drive its plungers The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended Early Maintenance Feedback EMF The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use them see Agilent Lab Advisor 12 1200 Series Binary Pump A User Manual Introduction 1 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mech
64. oval and disassembly of pump head A left one For pump head B right one proceed in the same way and skip steps that deal with the purge valve 1 Remove the front cover 1200 Series Binary Pump A User Manual Maintenance 7 Simple Repairs 2 Disconnect the capillary at the pumphead adapter and the tube at the active inlet valve Beware of leaking solvents Pumphead adapter Active inlet valve 3 Using a 3 mm hexagonal key loosen the purge valve holder and lift it up 4 Disconnect the Active Inlet Valve cable 5 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws Pumphead screws 6 Remove the pump head from the pump drive 1200 Series Binary Pump A User Manual 93 7 Maintenance Exchanging the Pump Seals When Tools required Parts required Preparations 94 Seals leaking if indicated by the results of the pump test check both pump heads individually 3 mm hexagonal key 4 mm hexagonal key 1 4 inch wrench Part number Description 2 5063 6589 Seals pack of 2 standard or 0905 1420 for normal phase application 5022 2159 For the seal wear in procedure Restriction capillary Switch off binary pump at power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 92 Disassemble the Pumphead assembly Using one of the plungers carefully remove the seal from the pump head be careful not to break the
65. owish white deposits on filters Typically black particles from the 1200 Series Binary Pump A User Manual Using the Binary Pump 4 normal wear of the piston seals do not cause the PTFE frits to block over short term usage Please refer to the section Exchanging the Purge Valve Frit or the Purge Valve on page 88 in this manual Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle part number 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1200 Series Binary Pump A User Manual 55 4 Using the Binary Pump Algae Growth in HPLC Systems 56 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 5 Optimizing Performance When to Use a Vacuum Degasser 58 When
66. p to injection device length 900 mm ID 0 17 mm Seal insert tool Wrench 1 4 5 16 inch Wrench 14 mm Hex key 4mm Corrugated Waste Tube 1 2 m Corrugated Waste tube reorder number 5 m Velocity regulator reorder number pack of 3 PTFE Frit G1329 87300 01018 23702 8710 0510 8710 1924 8710 2392 no PN 5062 2463 5062 2486 01018 22707 1200 Series Binary Pump A User Manual Installing the Pump 3 Optimizing the Stack Configuration If your binary pump is part of a complete Agilent 1200 Series system you can ensure optimum performance by using the configuration of the system stack in the following configuration This configuration optimizes the system flow path ensuring minimum delay volume Flow connections in the stack Example setup with 0 17 mm ID green capillaries Solvent bottle pump G1311 60003 bottle head assembly PTFE tubings Pump autosampler Solvent cabinet G1312 67305 SST green T Pump purge valve waste Degasser r 5062 2461 PTFE tubing wide bore L reorder pack Binary pump Instant Pilot G4208A Autosampler Autosampler column compartment G1313 87305 SST green Column compartment column G1316 87300 SST green Column detector DAD G1315 87311 SST coated VWD 5062 8522 PEEK Column compartment Detector waste DAD 0890 1713 PTFE wide bore VWD 5062 8535 PEEK 5062 2463
67. paratus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 1200 Series Binary Pump A User Manual 127 9 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 128 Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic house
68. plication Table 6 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between lsopropanol Best solvent to flush air out of reverse phase and normal the system phase both times After an installation Ethanol or Methanol Alternative to lsopropanol second choice if no Isopropanol is available To clean the system when Bidistilled water Best solvent to re dissolve using buffers buffer cristals After a solvent change Bidistilled water Best solvent to re dissolve buffer cristals After the installation of normal Hexane 5 lsopropanol Good wetting properties phase seals P N 0905 1420 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 50 Solvent Information 52 Prevent Blocking of Solvent Filters 53 Algae Growth in HPLC Systems 54 How to Prevent and or Reduce the Algae Problem 55 gy Agilent Technologies 49 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 50 Place solvent cabinet with the solvent bottles always on top or at a higher level of the binary pump When using the binary pump without vacuum degasser shortly degass your solvents for example apply vacuum for 15 30 s in an appropriate vessel before using them in the binary pump If possible apply solvent
69. plunger Remove wear retainers if still present Plunger ea 1200 Series Binary Pump A User Manual Maintenance 7 3 Insert seals into the pump head and press firmly in position Seal 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 104 Reset the seal wear counter and liquimeter as described in the User Interface documentation Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 but it will definitely damage the normal phase application seals 0905 1420 1 Place a bottle with 100 ml of isopropanol in the solvent cabinet and place the tubing including bottle head assembly of the pump head that is supposed to be worn in into the bottle 2 Screw the adapter 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it 3 Connect the restriction capillary 5022 2159 to the purge valve Insert its other end into a waste container 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min 1200 Series Binary Pump A User Manual 95 7 Maintenance 5 Close the purge valve set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 minutes at this pressure to wear in the seals The pressure can be monitored at your analog output signal with the handheld controller Chemstation or any other controlling device connected to your pump 6 Turn OF
70. power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1200 Series Binary Pump A User Manual 73
71. put or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch See your software documentation for further information The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series Binary Pump A User Manual Introduction 1 Electrical Connections Configuration switch GPIB Slot for interface board RS232 Remote Analog output CAN Bus er Power Plug Security Lever Figure 4 Rear View of Binary Pump Electrical Connections and Label 1200 Series Binary Pump A User Manual 15
72. r Active inlet valve cable 1200 Series Binary Pump A User Manual 105 7 Maintenance Exchanging the Optional Interface Board When Board defective Parts required Description 1 BCD Interface board see BCD External Contact Board in the Service Manual CAUTION Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1 Switch OFF the module at the main power switch Unplug the module from main power Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the module Install the new interface board Secure screws 2 3 4 5 Reconnect the cables to the board connector BCD Interface board Figure 27 Exchanging the Interface Board 106 1200 Series Binary Pump A User Manual Maintenance 7 Replacing the Module s Firmware When Tools required Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware mig
73. rations Part number Description 1 Pump 1 Power cord for other cables see text below and Cable Overview in the Service Manual 1 G4208A Control Software ChemStation EZChrom OL etc 1 G1323B and or a handheld controller Instant Pilot or Control Module Locate bench space Provide power connections Unpack the pump Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 32 1 Place the module on the bench in a horizontal position 2 Ensure the power switches on the front of the modules are OFF switches stand out 1200 Series Binary Pump A User Manual Installing the Pump 3 Installing the Binary Pump 3 At the
74. rear of the binary pump move the security lever to its maximum right position Status lamp Power switch Serial number Figure 7 Front of Binary Pump 4 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 1200 Series Binary Pump A User Manual 33 3 Installing the Pump Installing the Binary Pump 5 Connect the required interface cables to the rear of the binary pump see Connecting Modules and Control Software on page 36 Configuration switch GPIB Slot for interface board RS232 Remote Analog output CAN Bus tfetelTetstalrye we o o p o 1 mutoto EE Party OKON a n MANAMI wes censors IMEI 77 Power plug Security lever Figure 8 Rear of Binary Pump 6 Connect the capillary solvent tubes and waste tubings see Flow Connections of the Binary Pump with Solvent Selection Valve on page 39 or Flow Connections of the Binary Pump without Solvent Selection Valve on page 42 7 Press power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 8 Purge the binary pump see Priming and Purging the System on page 45 34 1200 Series Binary
75. remote connector at the rear of the module 4 Connect the APG cable to the APG remote connector of the pump see Figure 6 on page 30 5 Press in the power switches to turn on the vacuum degasser The AUX output allows the user to monitor the vacuum level in the degasser chamber 1200 Series Binary Pump A User Manual 37 3 38 Installing the Pump Connecting control software and or control modules 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 Plug a GPIB cable into the GPIB connector at one of the modules preferably at the detector MUST for the DAD 3 Connect the GPIB cable to the Agilent control software in use 4 Plug a CAN cable into the CAN connector of the control module Do not connect the Agilent control software or the control module with the vacuum degasser 5 Connect the CAN cable to the CAN connector of one of the modules 6 Press in the power switches to turn on the modules The Agilent control software e g ChemStation EZChrom OL etc can be also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the control module or Agilent control software refer to the respective user manual For connecting the Agilent 1200 Series equipment to non Agilent 1200 Series equipment see Introduction to the Binary Pump on page 6 1200 Series Binary Pump A User Manual Installin
76. rench unscrew the purge valve and remove it from the purge valve holder 4 Remove the plastic cap with the gold seal from the purge valve 5 Using a pair of tweezers or a toothpick remove the frit Valve body PTFE frit Gold seal Plastic cap Figure 20 Purge Valve Parts 88 1200 Series Binary Pump A User Manual Maintenance 7 Simple Repairs 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Replace the cap with the gold seal NOTE Before reinstallation always check the gold seal A deformed seal should be exchanged 8 Insert the purge valve into the purge valve holder and locate the pump outlet and the waste outlet as shown below 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Pump outlet capillary Purge valve holder Purge valve Waste capillary Figure 21 Exchanging the Purge Valve Frit 1200 Series Binary Pump A User Manual 89 7 Maintenance Exchanging the Solvent Selection Valve When If leaking internally croossflow between the ports or if one of the channels is blocked Tools required Screwdriver Pozidriv 1 Parts required Part number Description 1 G1312 60000 Solvent selection valve PN gives half of a complete solvent selection block 1 Disconnect the solvent tubes Place solvent tubes into the solvent cabinet to prevent leaks due to hydrostatic flow Disconnect the active inlet valve connection tubes from th
77. rs 11 Reconnect the Active Inlet Valve cable to the connector at the Z panel and the inlet tube to the valve 12 Reinstall the front cover Active inlet valve body Valve cartridge Figure 17 Active Inlet Valve Parts NOTE After an exchange of the valve cartridge it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly 1200 Series Binary Pump A User Manual 85 7 Maintenance Exchanging the Outlet Ball Valve Sieve or the Complete Valve When Tools required Parts required Preparations 86 Sieve whenever the pump seals will be exchanged Valve if internally leaking Wrench 1 4 inch Wrench 14 mm Part number Description 1 G1312 60008 Outlet ball valve 1 5063 6505 Sieve pack of 10 Switch off pump at the main power switch and unplug the power cable Before exchanging the outlet ball valve you can try to clean it in a sonic bath Remove the gold seal and the sieve Place the valve in upright position onto the plastic cap in a small beaker with alcohol Place in a sonic bath for 5 to 10 minutes Insert a new sieve and replace the gold seal 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body Remove the plastic cap with the gold seal 4 Using a pair of
78. sive diagnostics error detection and display through handheld controllers G4208A G1323B and Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable For use with flow rates below 500 l min a vacuum degasser is required 24 1200 Series Binary Pump A User Manual 1200 Series Binary Pump A User Manual 3 Installing the Pump Unpacking the Binary Pump 26 Damaged Packaging 26 Delivery Checklist 27 Optimizing the Stack Configuration 29 Installing the Binary Pump 32 Connecting Modules and Control Software 36 Connecting Agilent 1200 Series modules 36 Connecting an Agilent 1200 Series Vacuum Degasser 37 Connecting control software and or control modules 38 Flow Connections of the Binary Pump with Solvent Selection Valve 39 Flow Connections of the Binary Pump without Solvent Selection Valve 42 Priming and Purging the System 45 Priming with a Syringe 46 Priming with the Pump 48 ee Agilent Technologies 25 3 Installing the Pump Unpacking the Binary Pump 26 Damaged Packaging Upon receipt of your module inspect the shipping containers for any signs of
79. ssing is not included but a vacuum degasser is available as a separate product for applications that require best flow stability especially at low flow rates or maximum detector sensitivity This is most likely required to run small internal diameter columns 2 mm and 1 mm i d which require low flow rates A solvent selection valve optional will allow to select a binary mixture isocratic and gradient from four independent solvent bottles An active seal wash optional is available when the pump is used with concentrated buffer solutions Power supply HPM board Pump drive Mixer Damper Outlet valve Pump head A Mixing chamber Purge valve Solvent selection valve amp Pump head B Active inlet valve Leak sensor Figure 1 Overview of the Binary Pump 6 1200 Series Binary Pump A User Manual Introduction 1 Overview of the Binary Pump The binary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by two pump assemblies which can generate pressure up to 400 bar Each channel comprises a pump assembly including pump drive pump head active inlet valve which has a replaceable cartridge and outlet valve Both channels are connected in a low volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer A purge
80. to use the Seal Wash Option 59 When to Use Alternative Seals 60 When to Remove the Static Mixer 61 How to Optimize the Compressibility Compensation Setting 62 ee Agilent Technologies 57 5 Optimizing Performance When to Use a Vacuum Degasser 58 The pump does not necessarily require degassing But for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 ml min Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser lines are empty you should prime the vacuum degasser before running an analysis The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with a syringe is recommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 3 5 ml min is recommended when e pump was turned off for a length of time for example during
81. tor 0380 0643 5 Power supply behind rear panel 0950 2528 6 Screw M4 7 mm lg power supply 0515 0910 7 Washer power supply 2190 0409 1200 Series Binary Pump A User Manual 113 8 Parts and Materials for Maintenance Pump Head Assembly Table 12 Pump Head Assembly Item Description Part Number z a 7 Complete assembly included items marked with Sapphire plunger Plunger housing including springs Support ring Seal pack of 2 or Seal pack of 2 for normal phase applications Capillary outlet valve to piston 2 Pump chamber housing Active inlet valve body Replacement cartridge for active inlet valve 400 bar Screw M4 purge valve holder Purge valve holder Outlet ball valve Screw lock Apdater Purge valve assembly Screw M5 60 mm lg G1311 60004 5063 6586 G1311 60002 5001 3739 5063 6589 0905 1420 G1312 67300 G1311 25200 G1312 60025 5062 8562 0515 0175 G1312 23200 G1312 60012 5042 1303 G1312 23201 G1311 60009 0515 2118 114 1200 Series Binary Pump A User Manual Parts and Materials for Maintenance 8 Pump Head Assembly Figure 30 Pump Head Assembly 1200 Series Binary Pump A User Manual 115 Parts and Materials for Maintenance Pump Head Assembly with Seal Wash 116 Table 13 Pump Head Assembly with Seal Wash Item Description Part Number 1 o 3 19 Complete assembly included items marked with Sapphire plu
82. tweezers remove the sieve Check the gold seal It should be exchanged when strongly deformed Place the valve in an upright position insert the sieve into the recess and replace the gold seal with the cap Make sure that the sieve cannot move and is away from the seal area of the gold seal 1200 Series Binary Pump A User Manual Maintenance Simple Repairs 7 5 Place a new sieve into the recess of the outlet ball valve and replace the cap with the gold seal Valve body Sieve Gold seal Plastic cap Figure 18 Outlet Ball Valve Parts 6 Reinstall the outlet ball valve and tighten the valve 7 Reconnect the valve capillary Valve capillary Outlet ball valve Figure 19 Exchanging the Outlet Ball Valve 1200 Series Binary Pump A User Manual 87 7 Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 ml min of H30 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 14 mm Pair of tweezers or toothpick Parts required Part number Description 5 01018 22707 PTFE frit pack of 5 1 G1311 60009 Purge valve 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm w
83. ure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 124 1200 Series Binary Pump A User Manual Appendix 9 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many

G1312-90006 - Agilent Technologies

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