MRXe Microplate Reader User Manual
Contents
1. 15 Fitting Lamp and Optical 15 Switching the Reader ERR 17 Chapter 3 20 3 1 Routine Maintenance Procedures 22 23 Autocla 23 External Painted and Plastic 23 loris MCN EE 23 Decontatinauom 23 3 3 BRepaelkane the Reader 24 34 Replacmg the amps ota eie ebd eet 25 3 5 Changing the Optical 26 Appendix A Self Test Diagnostics eere 28 Self Test Sample Printouts 29 Appendix B Accessories 30 Appendix C Specifications e eeee eere eren 32 Softwate Specifications Uu Ma avra ped 32 Hardware Specifications enc ket prote 32 Performance iode eeu te t sue 32 405 to 850nm Wavelength Range eese 33 340 to 400 Wavelength Range 33 33 MRX Microplate Reader User Manual rte Physical Environmental Conditions seen 35 Uto rom ERNST MRX Microplate Reader User Manual About This Manual About This Manual This manual has been written for the laboratory technicia2. Appendix B Accessories Appendix B Accessories Description DYNEX Technologies Part Number Cable USB type A B 50600167 US Power Cord 6090405009 Euro Power Cord 6090405008 Asia Aust Power Cord 575100900 Filter 340nm 6329780340 Filter 405nm 6329780405 Filter 450nm 6329780450 Filter 490nm 6329780490 Filter 540nm 6329780540 Filter 550nm 6329780550 Filter 620nm 6329780620 Filter 630nm 6329780630 Lamp UV 549000600 Dust Cover 9119780011 Lens Cleaning Cloth 41000070 Service Manual 99000500 Verification Test Plate kit non UV 0119780004 MRX Microplate Reader User Manual Appendix Accessories DYN X EQUIPMENT IN TRANSIT IMPORTANT Please include copy of uus feri with eun LE vous nsirumesent contains 3 hard drive piease retain back up copies ot any files Failure bo do so may result in the lass af those files Ketum Authorization Number Conse Technical Service D YNEX Technologies phone 170353 E TEMO fox 703 ROLE TR L6 Equipment Scnal Nurnber EQUIPMENT DECLARATION Clearly indicate fault condition ar reason for return CERTIFICATE OF DECONTAMINATION certify iha the equipment described above has been disinfected decontaminaed and is clean drv aral fit for transport Shamed Title Date reserves the nghi te refuse improperly cleaned exgaipeseni Shipping Address DYNEX Technologies Abin Above reium namber 14350 Sullyfield Circ
3. These cover A D Status Begins conversion and checks that the status line goes high and low Verifies that hardware can be detected and chosen Plate Motor Moves the plate to Out position and then to the In position Verifies that the plate carrier position sensors are operating correctly e Checks motor and sensors Background Checks for light leakage into the reading Light compartment Lamp Verifies that current is flowing through the lamp when it is switched on Checks that the lamp has not blown Filter Motor Moves the filter motor to the first position Checks the filter motor and optical alignment Transmission Verifies that all fibers are capable of transmitting Levels at least 33 of the maximum transmission level e Checks for blocked channels or broken diodes Filters Checks that gains can be selected for all filters Checks for damaged or missing filters 28 MRX Microplate Reader User Manual Appendix Self Test Diagnostics Self Test Sample Printouts SELF DIAGNOSTIC SEQUENCE Reader Serial 4 2CXC2549 Technologist Date 3 17 98 Time 11 15 02 AM Test 1 A D Pass Test 2 Plate motor Pass Test 3 Background light levels Pass Test 4 Bulb Pass Test 5 Filter motor Pass Test 6 Transmission levels Pass Test 7 Filters Pass Self Diagnostic Summary Reader Status Ready MRX Microplate Reader User Manual 29
4. vibration drafts and away from direct sunlight Retain the packing materials in case future transport is required Figure 2 Packing Contents Example 1 Reader 5 Software CD 2 User CD 6 Power Cord 3 Optical Filters 7 USB Interface Cable 4 Lamp MRX Microplate Reader User Manual 11 Chapter 2 1 Installation The MRX Back Panel Mains Inlet 6 Optics Cooling Vents 2 Voltage Selector 7 Serial Number Plate See the Voltage Selector section below 3 Aux Mains Socket 8 PSU Cooling Vents 4 USB Port 9 Main Enclosure 5 Optics Door 1 The warning symbols back panel indicate that Notes the optics door may be hot and that no appliances other than a monitor should be plugged into the mains outlet For more details refer to the discussion on Symbols earlier in this manual 2 Remove the protective foam packing from inside the optics chamber MRX Microplate Reader User Manual Chapter 2 1 Installation Voltage Selector Switch The position of the voltage selector switch see Figure 3 is very important and must be adjusted to match the line voltage of the mains power supply before switching the Reader on Position of Voltage Selector Mains Line Voltage 115 100 120V 230V 200 240V CAUTION FAILURE TO FOLLOW THE PROCEDURES DETAILED BELOW MAY RESULT IN DAMAGE TO THE MICROPLATE READER DUE TO PARTS FALLING INTO THE POWER SUPPLY Voltage selector switch e If the voltage selector is not in
5. IT PEN MRXS Microplate Reader User Manual IMPORTANT Please read carefully before using the MRX Microplate Reader Rev A Part No 99000210 Software Version Instrument N This document is copyright of DYNEX Technologies Inc and must not be copied or reproduced in any form without prior consent DYNEX Technologies reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development This manual supersedes all previous editions is a trademark of DYNEX Technologies MRX is a registered trademark of DYNEX Technologies Microsoft Excel Windows registered trademarks of Microsoft Corporation CopyrightO 2007 DYNEX Technologies rights reserved Limitations of Warranty Relating to Software 1 DYNEX Technologies accepts no liability for the maintenance or support of Microsoft software 2 DYNEX Technologies accepts no liability for any consequential loss arising from the use of software on this product outside the scope of the above definition Limitations of Use e The user of the MRX must follow the specific manufacturer s assay package insert when modifying parameters and establishing results calculation methods e The performance characteristics of have not been established with specific serology or antigen detection assays The user must evalu
6. age selector see Figure 3 The position of the selector is very important and must be adjusted to match the line voltage of the mains power supply Position of Voltage Selector Mains Line Voltage 115 100 120V 230V 200 240V If the voltage selector is not in the correct position refer to the Voltage Selector discussion on page 13 DO NOT place any objects or stand within 150mm of the front of the Reader as this will obstruct the plate carrier 1 Make sure that the instrument is switched OFF The power switch is ON when it is flush with the front panel and OFF when it stands out from the panel 2 Insert the power cord supplied into the socket at the rear of the instrument 3 Connect the power cord to a suitable power source with a protective earth ground 4 Connect the supplied USB cable to the and the personal computer used to control the reader Switch Reader on by pressing power switch on front panel 6 Power up the computer and install the Revelation operating program by running the file SETUP EXE located on the disk MRX Microplate Reader User Manual 17 Chapter 2 1 Installation Self Tests When the Revelation program starts on the computer the unit carries out several self tests to check that the instrument is working correctly Appendix A shows a list of these tests and a sample printout If the Reader fails any of the tests an error message is displayed If the software ca
7. ate the MRX in conjunction with the specific serology and or antigen detection assays This evaluation must include the establishment of performance characteristics for the specific assays Under no circumstances does MRX have any direct patient contact or perform any therapeutic patient function Specific diagnostic results are not provided by the device Diagnostic decisions are made only after independent confirmation by additional methods under the supervision of a qualified professional WARNING If this equipment is used in a manner not specified by the manufacturer then the protection provided by the equipment may be impaired Before using any decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment This page is intentionally left blank Table of Contents Table of Contents About This Manual 1 Safety Informa ooo 3 SY MUD T E 5 Symbols 5 Chapter 1 Features of Reader 7 Chapter 2 Installation and Setup ees 9 2 1 Installation 11 Unpackine the ad aduer eda lebe cele ahve 11 The Back Panel 12 Voltage 13 USB Driver Installatot OS 13 Panel A
8. cently the optics chamber door and the lamp in the optics assembly may still be hot Allow time for these components to cool before handling Take care when removing the optics chamber door as there may be glass from a possibly broken lamp in the optics assembly See Figure 10 Note DO NOT touch the filters lamp or reflector with bare fingers Switch off the power supply and disconnect the mains cable Using a Phillips screw driver remove the optics door 1 2 3 Slide optics assembly out of instrument 4 Remove the relevant filter from its position in the filter wheel and replace with the new one Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp Make sure the Filter Springs are around the sides of the Filter as shown in Figure 6 on page 14 The Springs must not extend past the rear face of the Filter as shown in Figure 7 on page 15 Otherwise the self test will fail due to Filter Motor Errors Slide the optics assembly back into the Reader chassis o Replace optics chamber door and reconnect power supply 26 MRX Microplate Reader User Manual 3 5 Changing Optical Filters This page is intentionally left blank MRX Microplate Reader User Manual 27 Appendix A Self Test Diagnostics Appendix A Self Test Diagnostics On power up and optionally before every read the Reader performs several self tests
9. ck Hazard 3 Equipment in Transit form decontamination See Appendix B External Painted and Plastic Surfaces cleaning the 22 Filters cleaning the 22 Front Panel 14 Fuses 4 Incorrect Operation 3 m Lamp fitting the 14 16 replacing the 24 M Maintenance 21 22 Microplate Reader See Reader front panel 14 36 e MRX back panel 12 O Optical filters changing the 25 Optical Filters fitting the 14 16 R Reader accessories 29 electrical specifications 32 features of 7 8 hardware specifications 31 interface 32 performance specifications 31 32 physical environmental conditions 34 repacking the 23 self tests 17 software specifications 31 switching it on 16 unpacking the 11 Repacking the Reader 23 S Safety Information 3 4 Self tests 17 27 sample printout 28 Specifications electrical 32 hardware 31 interface 32 performance 31 32 physical environmental 34 software 31 INI Voltage Selector 13 W Warning Symbol 5 MRX Microplate Reader User Manual
10. h a warning symbol to remind you of certain hazards the back panel this indicates that the optics door may be hot If you want to remove the optics door make sure that you allow the Reader to cool before touching it Adjacent to the mains outlet This indicates that the line voltage switch must be correctly set before plugging the unit into an outlet This also indicates that the auxilliary outlet has a power rating of up to 120V A and an earth leakage current of less than 1 mA at 264V and 60 Hz MRX Microplate Reader User Manual 5 This page is intentionally left blank MRX Microplate Reader User Manual Chapter 1 Features of the Reader Chapter 1 Features of the Reader The Microplate Reader is a microprocessor controlled photometer It is designed to measure the optical density OD of fluid samples in 96 well microplates in order to determine the results of chemical reactions Figure 1 MRX Microplate Reader A full list of the Reader s technical specifications can be found in Appendix C The Reader provides the following features Revelation software capable of detecting Endpoint Agglutination and Kinetic assays PC compatibility for control and data storage e Single dual and multi wavelength reading modes e Selection of up to six filter wavelengths e Data analysis and presentation as OD matrices and or graphics using curve fitting routines e Data output to a PC Verification P
11. ions contained in this manual or if the covers are removed by anyone other than approved service personnel 3 1 Routine Maintenance Procedures The Reader requires very little maintenance however certain simple procedures should be carried out on a regular basis On a daily basis e Verify self test passes Remove any microplate from the plate carrier wipe the plate carrier with a lint free cloth and return it to the In position Wipe the external surfaces Section 4 2 contains more detailed cleaning instructions On a weekly basis Review the results of the self test that occurs when you switch the Reader on to make sure the instrument is functioning correctly If you want to retain this information for your records print out the self test results see Appendix A for a sample or save them to a file On a yearly basis Remove the filters and clean them Section 3 2 contains more detailed cleaning instructions Periodic maintenance should be determined by the user depending upon location and environmental conditions 22 MRX Microplate Reader User Manual 3 1 Routine Maintenance Procedures 3 2 Cleaning Warning Always switch the instruments off and disconnect the power cable before cleaning the Reader The Reader is constructed from high quality materials nevertheless spilt saline solutions solvents acids or alkaline solutions must be removed from outer surfaces immediately to prevent damage Aut
12. late option MRX Microplate Reader User Manual 7 Chapter 1 Features of the Reader Temperature Control option 8 MRX Microplate Reader User Manual Chapter 2 Installation and Setup Chapter 2 Installation and Setup bonis cca 11 Wnpackine th Reader 11 The Reader Back Patel chica anda due ig ta ces dto ee 11 Voltage Selector 9 WIBCDL dente berries tee Doe cope el derer eere pes 13 USB D ver Installations s cii i eb rr e e Eee as eii ee OH ede 13 The Reader Front Patel e od e Waka ee Gh ed 15 Fitting the Lamp and Optical 15 Switching the Reader Oi 17 eidem aee bao 11 MRX Microplate Reader User Manual 9 Chapter 2 Installation and Setup This page is intentionally left blank 10 MRX Microplate Reader User Manual Chapter 2 1 Installation 2 1 Installation CAUTION DO NOT connect the instrument to the mains electrical supply or switch it on before you have completed this installation procedure Unpacking the Reader The Reader is packed to provide maximum protection during shipment Take care when unpacking it and examine it carefully for any damage Report any damage to the carrier immediately Check the contents against the Instrument Configuration Report enclosed and report any omissions to your supplier Remove the Reader from the carton and place it on a level surface free of dust moisture
13. le Chantally 2051 1583 Suzzesbed decontamination methods Wash all surfaces with a 108 bleach solution Follow thai wiih a mild detergent salistien Washers follow ie Deoantsmanatian Precocdbure found in the back af the mamal MRX Microplate Reader User Manual 31 Appendix Specifications Appendix C Specifications Software Specifications Standard Revelation Program Hardware Specifications No of filters 6 Performance Read cycle time Single Wavelength lt 4 seconds Dual Wavelength 6 seconds 32 MRX Microplate Reader User Manual Appendix Specifications 405 to 850nm Wavelength Range Dynamic range 0 100 to 4 000 OD Linearity 0 000 to 2 000 1 0 2 001 to 3 500 OD 1 5 Filter band width FWHM 10 2 Center Wavelength Accuracy 2nm Precision 0 000 to 2 000 OD 0 2 CV or 0 005 OD whichever is greater 2 001 to 3 000 OD 0 6 3 001 to 3 500 OD 1 0 CV Accuracy 0 000 to 3 500 OD 2 5 or 0 005 OD whichever is greater 340 to 400 Wavelength Range Option Dynamic range 0 100 to 3 000 OD Linearity 0 000 to 2 500 2 0 Filter band width FWHM 18nm 2 nm Center Wavelength Accuracy 2nm Precision 0 000 to 2 000 OD 0 6 CV or 0 005 OD whichever is greater 2 001 to 2 500 OD 1 0 CV Linearity is the maximum percentage deviation of a reading from the ideal straight line plot of OD versus conce
14. ment off press the power switch on the front panel Remove the mains power cable from the socket on the rear panel Warning Operating this equipment in ways other than detailed in this manual may Incorrect impair the protection provided by the instrument Operation DO NOT operate this equipment with the covers removed as potentially lethal voltages are contained within DO NOT operate this equipment with the safety earth ground disconnected DONOT install spare components or accessories as this may impair the safety of the Reader and will invalidate its warranty DO NOT place any objects or stand within 150mm of the front of the Reader as this will obstruct the plate carrier DO overfill the microplate wells as this may lead to liquid contamination of the wells and impair the safety of the Reader BEPREPARED for unexpected plate carrier movement MAKE SURE that the voltage ratings on the rear panel of the Reader correspond to the local mains supply MRX Microplate Reader User Manual 3 Safety Information MAKE SURE that the mains power cable is correctly wired Color codes are as follows Europe United States Brown Black Live Blue White Neutral Green yellow Earth ground Green Ground Fuses The instrument does not contain any user replaceable internal fuses MRX Microplate Reader User Manual Symbols Symbols Warning Symbol For your safety the Reader is marked wit
15. n and describes how to use the MRX Microplate Reader With the information in this manual you can Install the Reader Configure the Reader using the Setup dialog to suit your particular needs Connect the Reader to a PC personal computer Perform basic maintenance procedures This manual also describes all the features and specifications of the Reader hardware and software Information on how to use the Revelation software is included For explanations of error messages and possible solutions refer to the included Troubleshooting Guide Note Manual updates available at www DynexTechnologies com MRX Microplate Reader User Manual 1 This page is intentionally left blank MRX Microplate Reader User Manual Safety Information If there is any doubt or concern about the safety of the instrument contact an approved service center Warning Although this instrument is fully insulated and earthed grounded it is Electric important for all users to be aware of the potential hazard of using liquids Shock in close proximity to an electrical supply If any liquids are spilled Hazard disconnect the instrument from the mains electrical supply immediately and clean the liquid up DO NOT reconnect the electrical supply until the instrument has been fully inspected by an approved service engineer Take care when using non rigid non standard microplates as spillages are more likely to occur To switch this instru
16. n not communicate with the Reader it will request the unit s serial number this can be found on the Reader s back panel Double check the cable connection between the Reader and the PC If an error message persists contact the service center MRX Microplate Reader User Manual Chapter 2 1 Installation This page is intentionally left blank MRX Microplate Reader User Manual 19 Chapter 3 Maintenance Chapter 3 Maintenance 3 1 Routine Maintenance Procedures 22 cR 23 23 External Painted and Plastic Surfaces 23 noe Ri ct 23 Decontami at On ssec si eoa 23 Jed Repackiatig Reddere eie vv ae ERA RUN ER ERR AERE RUE 24 3 4 Replacing 25 3 5 Changing the Optical 26 20 MRX Microplate Reader User Manual Chapter 3 Maintenance This page is intentionally left blank MRX Microplate Reader User Manual 21 Chapter 3 Maintenance IMPORTANT The first section in this manual contains safety information for all the equipment in the system You should be familiar with this information anyway but if not you must read through this section before carrying out any maintenance The warranty on the Reader may become void if you do not follow the safety and maintenance instruct
17. nd caps into the carton and lower the Reader into the carton Fit the top end caps to the Reader Place the filters and lamp in the accessories box and fit this between the end caps Seal the box carefully and securely Make sure that the box is not dropped or handled in a rough manner Figure 8 Repacking the Reader 24 MRX Microplate Reader User Manual 3 3 Repacking Reader 3 4 Replacing the Lamp Warning If the Reader has been used recently the optics chamber door and the lamp in the optics assembly may still be hot Allow time for these components to cool before handling Take care when removing the optics chamber door as there may be glass from a possibly broken lamp in the optics assembly Figure 9 summarizes the lamp replacement instructions Note DO NOT touch the lamp or reflector with bare fingers 1 Switch off the mains power supply and disconnect the cable 2 Using a Phillips screw driver remove the optics door 3 Slide the optics assembly out of the instrument 4 Detach the lamp from the assembly and replace with a new one Make sure that the lamp is seated correctly and connected 5 Slide the optics assembly back into the Reader chassis 6 Replace the optics chamber door and reconnect the power supply Figure 9 Replacing the Lamp MRX Microplate Reader User Manual 25 otical Filters 3 5 Changing the Optical Filters Warning If the Reader has been used re
18. ntration Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range Precision is the mean Coefficient of Variation CV figure for all wells on a plate that has been read 20 times in succession Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range Interface Computer Interface USB 1 0 2 0 compatible Electrical Light source 75W tungsten halogen lamp MRX Microplate Reader User Manual 33 Appendix Specifications 34 Mains input Power consumption Fuses Mains Power Fuse U K mains plug powercord Safety 100 to 120V or 200 to 240V 50 to 60 Hz switch selectable 175 VA There are no internal user replaceable fuses The power supply contains a CSA approved 20 5 4A cartridge fuse with a voltage rating of 250V in the primary circuit If the fuse blows contact the service center 5A Class apparatus MRX Microplate Reader User Manual Appendix Specifications Physical Environmental Conditions Weight 10 kg 22 16 Dimensions D390 x W360 x H220 mm 15 4 x 14 2 x 8 7 inches Operating Temperature 10 40 Operating Humidity 10 90 RH Storage Temperature 0 50 C Storage Humidity 10 9096 RH Altitude 2000m MRX Microplate Reader User Manual 35 Appendix Specifications Index Accessories 29 p Back Panel 12 Cleaning 22 D Decontamination 22 Electric Sho
19. oclaving DO NOT autoclave any part of this instrument External Painted and Plastic Surfaces Clean external surfaces with a mild laboratory detergent followed by 1096 bleach or 70 alcohol Always dilute detergents according to the manufacturer s instructions Filters Clean the filters by wiping them with a lint free cloth or a lens tissue If necessary a little alcohol may be used with the cloth or tissue Decontamination If you need to return the Reader to the service center and it has been in contact with human blood other potentially infectious body fluids pathological samples toxic or radioactive materials it must be decontaminated Before shipping the instrument do the following e Clean the surface with a mild laboratory detergent followed 10 bleach Fill out an Equipment in Transit form refer to Appendix B for a copy of the form MRX Microplate Reader User Manual 23 3 2 Cleaning 3 3 Repacking the Reader This procedure assumes that the original Reader packaging materials are being used Figure 8 summarizes the repacking instructions D QA ax cux s Remove the optics chamber door and take out the lamp and filters When you replace the optics chamber door insert the optics chamber packing materials Pack the lamp and each of the filters separately Make sure that there is no microplate in the plate carrier Place the Reader in the original anti static plastic bag Place the bottom e
20. tch on the optics assembly MRX Microplate Reader User Manual 15 Chapter 2 1 Installation For Philips type lamps Make sure that the lamp is seated correctly and that the lamp pins are horizontal as shown in Figure 5 Figure 5 Fitting the Lamp and Filters Insert the filters into the filter wheel Place the filter with the lowest wavelength in position 1 the next lowest in position 2 and so on e Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp e Fit filter blanks into all unoccupied positions Make sure that the retaining springs grip the filter casing and are not bent forwards over the edge of the casing The Filter Blanks must be fitted into the unoccupied filters positions in the Filter Wheel Make sure the Filter Springs are around the sides of the Filter as shown in Figure 6 The Springs must not extend past the rear face of the Filter as shown in Figure 7 Otherwise the self test will fail due to Filter Motor Errors Figure 6 Correct Filter Spring Positioning view of Filter Wheel from filter spring side MRX Microplate Reader User Manual Chapter 2 1 Installation Figure 7 Incorrect Filter Spring Positioning 4 Slide the optics assembly back in attach the lamp connector to the lamp and replace the optics chamber door Switching the Reader On Before switching the Reader on note the position of the volt
21. the correct position insert a suitable small flat bladed screwdriver through the slot in the clear plastic cover and move the selector to the desired voltage DO NOT LOOSEN THE SCREWS USED TO RETAIN THE PLASTIC COVER USB Driver Installation The Reader requires the installation of a USB driver which emulates a standard 5 232 COM port on the control computer The port is then referred to as a Virtual Com Port VCP To start the installation process open the file USB Driver and VCP Install Procedure located on the supplied User CD Follow the procedure through to its completion before continuing MRX Microplate Reader User Manual 13 Chapter 2 1 Installation 14 MRX Microplate Reader User Manual Chapter 2 1 Installation MRX Front Panel Figure 4 The Front Panel 1 Power Switch marked I O for on off 2 Power Light 3 Plate Carrier Door Fitting the Lamp and Optical Filters Warning Disconnect the mains cable before fitting lamp and filters Note DO NOT touch the filters lamp or reflector directly 1 Using a Phillips screw driver remove the optics door and if fitted remove the packing materials from the optics chamber 2 Slide the optics assembly out of the instrument 3 Insert the lamp into the assembly and plug the connector onto the lamp pin contacts see Figure 5 For Osram type lamps Make sure that the lamp is seated correctly with the locating pip on the lamp fitted into the no
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