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1. 3 Check the large O ring seal in the grooved Ww 4 ru flange of the vessel glass is lightly greased not damaged and not wrinkled when in place Cleaning or Assembl 4 Charge vessel with water to approximately the required working volume of the vessel n i Check the electrode condition then 5 Replace the top plate and tighten retaining prepare the electrodes for first use clamp to finger tight Take care not to according to the instructions given accidentally clamp the autoclaving cover for in the relevant appendix i e the Pt 100 in the clamp Tighten all three screws on the clamping ring for even pH remove white sealant over pressure on the seal diaphragm completely 6 Equip any unused ports with a membrane collar and port closure if the port is to be used pO2 UNGIS base section for additions post autoclaving If not a remove diaphragm cartridge and blanking port closure is sufficient half fill with electrolyte Replace 7 Calibrate the pH electrode using the appropriate menu option in U DDC and buffers of eg pH 4 and 7 Follow the instructions to set a low and high reading 8 The dissolved oxygen electrode low value calibration can be set using the zeroing gel provided CARE strongly alkaline solution 9 Fit the pH and dissolved oxygen electrodes in the 13 5PG ports they have a finer thread than standard using adaptors if the elec
2. Numeric keys 1 9 plus Data Entry numbers 1 6 Select fermenter Toggles options Enter Confirmation Esc Abort last action UP ARROW Moves upwards DOWN ARROW Moves downwards Menu Go to Main Menu The screen is a high contrast LCD unit displaying 40 characters by 16 lines Different areas of the screen provide different sorts of information Top Line Fermenter data Second line Current option Lines 4 13 Fermenter values Lines 15 16 HELP information A square flashing cursor appears in a number of screens against something which can be changed STARTING OR STOPPING A FERMENTER To start a fermenter from the Main Menu select Option 1 fermenter Menu The screen changes to allow you to select which fermenter 1 6 to start using the numeric keypad The Fermenter Menu is then displayed Select Option1 Start Stop In the start Stop screen use the DOWN ARROW key to move through the options deciding which parameters to keep On and which to switch Off using the key Finally use the key to switch Start Fermenter to On and press either Enter or Menu to confirm Menu takes you back to Main Menu and pressing Esc will let you see the Values screen To stop an active fermenter select Option 1 Start Stop from the Fermenter Options menu and use the key to toggle Stop fermenter to On amp press Enter or Menu to confirm the shutdown CHANGING A SETPOINT There are two ways to c
3. 2 Gas Mix Kit Installation 1 Remove the back cover of the main pcb housing and drill a 4 5mm diameter hole in the cover 290mm from the bottom and 82mm from the left hand edge looking from the rear of the fermenter 2 Fit the bracket on the pcb holder itself brass coloured using the screw and nut from the black plate to the main pcb cover using the hole just drilled If correct the pcb etc will just fit into the cover 3 Remove the main flat band cable from the main pcb 25 way D connector to 20 way connector on pcb 4 Replace with the new cable by offering the rear cover up to the main pcb 5 Connect the single red wire to the corresponding red wire on the 24V connector on the main pcb to provide power 6 Pass the brown cable to the valves out from the main pcb using the cut outs on the bottom edge of the housing And carefully replace the cover do NOT force it Fitting of the valves 1 Remove the lead connector from the two gas valves by undoing the small cross head screw at the top of each valve retain rubber gaskets beneath the connector The mid section of each valve can also be removed to aid fitting undo the 2 small screws holding this section to the valve base 2 Undo the outlet pipe at the top of the rotameter 3 Undo the middle panel holding the rotameter and pull it gently forward to get access to the rear of the rotameter 4 Remove the two screws holding the rotameter in place and br
4. 3 more analogue outputs 0 10V or 4 20ma arranged in groups of 3 Analogue Box Front On the input block the left hand screw is for connecting the terminal Block signal line and the right hand for the signal ground On the output blocks the left hand screw is for connecting the 0 10V output the middle for 4 20mA output and the right hand screw for signal ground Back From the left hand edge looking from the rear 24V connections these are piggybacked onto the equivalent connectors on the main pcb Serial IN goes to base 25 D connection on the Master unit Serial OUT not used unless linked to satellite units Analogue I O linked to position 4 on main pcb Fitting 1 Remove cover on main pcb housing at rear of Labfors 2 Connect 24V power leads to 24V connector on main pcb 3 Connect analogue cable to position 4 on main pcb count from top 4 Connect serial link to 25 way D connector on base unit of the Master Labfors 5 Fit special EPROM for operation with analogue box 6 Replace cover and pass cables etc through the semi circular slots cut into bottom edge of the main pcb housing 7 Use terminal block on front of box to connect the signal and ground lines of any peripheral equipment eg input from a turbidity meter Analogue cable connection 8 Connect any output signals from the box to the relevant peripheral eg an analogue peristaltic pump Set up display calibration on screen see opera
5. esp P term default values available Page 19 LABFORS SERVICE MANUAL 5 4 pH System No display or incorrect display of pH No pH Control pH drifts up or down over time or acid amp alkali added almost continuously in turn Service Manual Version 1 0 for LABFORS Possible Causes Faulty pH electrode Temp compensation OFF Faulty amplifier module Processor problem Control not active Pumps not set to Auto No control in dead band No reagents Pumps not operating properly If pump heads do not rotate Faulty pump motor Wrong tubing type Faulty Solenoid Is only one pump effected If YES Control output not given Adjust set points Check indicator LED s do the acid and base pumps lights ON If NO Processor Fault Check Fermenter Overview does this show acid alkali pumps active lf NO PID settings incorrect Incorrect strength of reagents Remedy Check connections Test calibration with pH 4 and ph7 buffers Check slope of electrode for signs of ageing Replace or regenerate electrode Turn this to ON in normal use Check connections Replace sensor or use simulator fo test Replace amplifier module Switch OFF and ON again Replace processor Ensure control is turned ON Check and set to Auto if necessary pumps submenu Ensure this is OFF or small value Ensure bottles full line open amp connected to vessel Check operation manually and prime pumps Che
6. 57 IRIS Software Connections to Labfors 6 Repair items 6 1 Testing of process parameters setup calibration actuators 6 2 Removal of covers basic sections of the Labfors 6 3 Exchange of Pt 100 sensor 6 4 Exchange of circulation pump if applicable 6 5 Exchange of heating element mantle 6 6 Exchange of small heater pcb 6 7 Exchange of drive motor 6 8 Motor control pcb adjustment amp replacement 6 9 Exchange of rotameter inner cylinder 6 10 Exchange of operational amplifier module 6 11 Exchange of digital distribution pcb in 3rd section 6 12 Exchange of peristaltic pump components 6 138 Exchange of main pcb eprom micro processor 6 14 Exchange of screen amp keypad 7 Installation of Optional Accessories Post delivery 7 1 Addition of analogue box amp linking to external peripherals 7 2 Addition of satellite unit to Master system Lo Addition of mass flow controller and full gas mix system 7 4 Addition of two gas mix system lo Linking to IRIS software troubleshooting connection setup Page Service Manual Version 1 0 for LABFORS Page 2 WHERE TO LOOK BASIC INFORMATION INSTALLING A NEW SYSTEM CLEANING A UNIT ROUTINE EXCHANGE OF CONSUMABLES WHAT COULD BE WRONG HOW TO EXCHANGE KEY COMPONENTS HOW TO ADD ADDITIONAL ITEMS LABFORS SERVICE MANUAL 8 Appendices SPECIFIC 8 1 PID drawings BigOver1 doc INFORMATION AS 8 2 Electrical schematics Elecsch paf INDIVIDUAL SHEETS 8 3 Pump head flow rate deta
7. E 7 000 High Reading 2048 Low Ref E 4 1 Low Reading Slope 0 00379 Offset 11 98 Sensror Data Act read 0612 Act Value 0 00 Slp mV pH 56 3 Ref Temp 24 3 a gt Fl lt STATUS OK 00d13 00 gt Pump Flow Settings Fermenter F1 Acid Pump Auto Cycle 60 Set 0 Base Pump Auto Cycle 60 Set 24 AF Pump Auto Cycle 60 Set 0 Feed Pump ON Cycle 60 Set 18 Flow Out Auto Value 1 min O Select Option Menu for Main Menu nter or Esc to exit Fermenter gt Fl lt Status OK 00d00 00 pO2 Control Def Fermenter F1 Flow Cont On RPM Cont On Max Infl 100 Gas Mix Off Hand Mix 0 Menu for Main Menu to change value Enter Esc to exit Service Manual Version 1 0 for LABFORS Page 11 LABFORS SERVICE MANUAL 3 Cleaning Before disconnecting the corrective reagent feed lines from the Mains switch peristaltic pumps they should be clamped off They can then be On the right hand side of the base unit emptied manually after re sterilization OFF On safety grounds the fermenter must be switched off and the drive motor removed before opening the vessel Cleaning the base unit and Master Control panel 1 Use a soft damp NOT WET cloth anda neutral detergent to wipe away any stains or grime 2 Clean the unit down with electrical power off and allow to dry completely before use 3 After a spillage the stainless steel tray at the bottom of the base unit may be removed
8. Flow Rate Manual Air OUT E e a i tile Rota meter Air IN Unless specified otherwise control of gas flow into the fermenter is usually set manually A separate pump or laboratory air supply is connected to the fermenter supply pipework which has an integral pressure regulator valve The flow rate is adjusted using a rotameter calibrated in litres per minute hour The maximum air flow rate should be no more than 1 5 vessel volumes per minute A mass flow valve and or gas mixing system may also be used CHEMOSTAT OR CONTINUOUS OPERATION optional Probe Medium IN INPUT OUTPUT ON PERAR Feed Pump OFF Shot amp 50Secs Delay Keypad Settings or Analogue Or Analogue Medium OUT INPUT OUTPUT en fo Harvest 3 Pump This relies on a balance being set between addition of fresh medium into the vessel and removal of culture The correct rate matches the growth of the microbes so that a constant concentration in the vessel is maintained If the growth of the organism is limited by eg a source of sugar in the medium then the fermenter is being operated as a chemostat Medium is added to the culture by a peristaltic pump which can be an integrated digital unit relying on shot and delay times or an external analogue pump with variable speed Harvest of culture can be achieved by using a level cont
9. If pumps or valves are involved make sure these are switched to Auto or have realistic manual set valves 9 Monitor the action of controllers via the Fermenter Overview screen and LED indicators in the third section 10 Use your senses for additional verification of actuators working eg if the heater works the vessel will get hot or you may be able to hear solenoid valves click on or off 11 Some parameters take a long time to get to fully operational condition eg dissolved oxygen electrode needs at least 2 hours with power supplied Don t take the first value as correct especially soon after autoclaving 12 Look at measured values and the raw A D converter readings on the calibration page do they roughly match eg pH7 approx 512 an A D reading of 100 or 1000 would suggest a fault see addendum for more info 13 Unexpected values or odd behaviour from measurement probes can sometimes indicate an earth problem which may not show up on a conventional electrical test eg the dissolved oxygen electrode is very sensitive to a poor earth connection leading to the value drifting high Default values for calibration and settings are provided within the EPROM for re initialised cards and new installations see separate addendum UDDC installation information sheet The next stage in fault investigation usually requires you gain access to the relevant actuator for more direct tests CHECK COMPONENTS BY SWAPPING WITH THOSE IN A
10. and replace the cover 6 10 Exchange of the operational amplifier pcb This is located in the third section and is connected to the front panel which comes away when the screws are removed The electrode cables terminate at the left side of the panel and link to the operational amplifier by a D connector The purpose of the pcb is to digitise the analogue signals from the electrodes Exchange may be necessary if eg a sensor value cannot be calibrated properly even with a simulator Check the connections from the electrode cap and cable wiring before reaching this conclusion To exchange the pcb 1 Remove the front cover by undoing four screws 2 left 2 right 2 Remove the earth lead from the front panel 3 Remove the ribbon cable connector at the rear of the module 4 Rest the panel on its front to gain access to the amplifier module 5 Undo the two screws holding the metal protective shield and pull this backwards to expose the pcb part 19939 plug in measuring unit 6 Remove the pcb from the D connector where it links to the wiring from each of the four sensor cables 7 Replace the pcb 8 When replacing the cover lift the back edge slightly to allow the connector for the ribbon cable to pass through 9 Replace the ribbon cable connector 10 Replace the pcb To adjust antifoam sensitivity Remove the Op amp board Remove the top cover Remove the ribbon cable to free the cover then replace At the front of th
11. braided silicone tubing used for these connections 10 Release the pump from its supporting backplate by unscrewing the retaining screws Service Manual Version 1 0 for LABFORS Page 26 LABFORS SERVICE MANUAL 11 Remove the end fittings from the faulty pumps and re fit them on the new pump 12 Check the braided silicone tubing for signs of wear and replace if necessary part no 10454 13 Re assembly is the reverse of this procedure 6 5 Exchange of Heating element The indication of a need for exchange will be 1 No heating maybe a dead short on the element also check using a multi meter 2 Water circulates 3 The controller output indicates heating is called for 4 There is power entering and leaving the connections on the Aqua pcb to the heater white amp white striped cables To exchange the heating element 1 Remove the screws supporting the bottom section of the base unit 3 at the rear left 3 rear right 2 Gently lift the bottom section forward and outwards a short way 3 Remove the earth leads Heating element amp 4 Reach inside and remove the small ribbon cable coming Wiring from the base unit onto the middle of the Aqua pcb part no20263 5 The front section can now be turned clear of the main body of the base unit to allow easy access 6 Locate the heater cartridge part no 13719 7 Remove the two wires from the heater element to the Aqua pcb white and white striped fabric no
12. damage to the glass front 2 Remove the inner guide for the memory card from the main pcb housing 2 x 7mm nuts 3 Remove the four large nuts holding the back cover of the screen keypad housing 4 Bring cover up gently and remove the earth lead on the inside of the back cover 5 Remove the back cover and the pcbs on the inside of the screen keypad can be seen N B If removed without taking the rear pcb housing off the front of the screen keypad housing can be removed only by disconnecting all cables plus earth leads and undoing the 4 Screen amp Keypad Interior large nuts at the rear The front of the screen keypad housing can then be lifted clear from the front of the Labfors Service Manual Version 1 0 for LABFORS Page 35 LABFORS SERVICE MANUAL 6 14 Replacing Screen and or Keypad ae 2 Place glass screen face down on a cloth for protection Undo the 6 small nuts holding the LCD screen pcb on right Retain all spacers and ceramic washers Undo the earth lead at the bottom of the housing Disconnect small connector at the bottom of the keypad pcb which links two white leads from the LCD pcb to the small perpendicular pcb across the keypad area Lift LCD panel and remove replace as necessary Remove the small perpendicular pcb by undoing two small retaining nuts The keypad area can now be removed if required Re assembly i 2 HO 11 ize Fit the earth lead to the lower left hand si
13. face O ring Soft face Seal Faces Assembled Hard Face rotates Soft Face static Service Manual Version 1 0 for LABFORS Page 15 LABFORS SERVICE MANUAL 4 5 Battery on Memory Card The memory card stores information about setpoints calibration PID setup profiles and other preferences A watch batttery preserves tha data on the card even when the Labfors is switched off After a period of 1 2 years the battery loses power and the user reports a loss of settings every time the unit is switched off A further reason for wishing to remove the battery may be that a PIN number password has been set and the user has forgotten it Removing the battery clears the memory The user will normally exchange the batitery themselves but may well ask for it to be done as part of preventive maintenance 1 Remove the card from the slot in the Labfors main pcb housing noting the orientation of the card 2 A small screw is found on the back edge of the card Remove this with the special tool provided with the card or using a watchamaker s screwdriver 3 Put your fingernail or eg a thin penknife blade into the small crack above the area containing the screw hole and pull backwards 4 A battery holder is revealed with the battery sitting in position Note the orientation of the battery and exchange for a new one TYPE XXXXXXX 5 Ensure the write protect tab on the back edge of the card is seet towards the midd
14. for corrosion and to replace clearly worn or damaged membranes and fittings 4 4 Mechanical Seal This is another compnent of vital importance for maintaining a contamination free culture It Cap Dip tube Air filter prevents microbes from entering or leaving via the rotating drive shaft The mechanical seal is subject to wear over time and is normally replaced Bottle Meade enh at annual service inspections if the customer requests this Any contamination of cultures which cannot be explained by the usual reaons of seed problems or O ring failure may be due to a fialure of the mechanical seal Mechanical Seal on Drive Shaft 1 To exchange the mechanical seal the vessel top plate must first be removed by unscrewing the bolt holding the clamping ring in place and then lifting the top plate free 2 Itis a good idea to remove the pH and dissolved oxygen electrodes first along with any other fittings which reduce access to the centre drive shaft 3 Allow the top plate to rest on its side so the underside can be clearly seen The mechanical seal is recessed into the drive shaft bearings housing and is held in place by a spring and locking collar which are fitted to the bottom of the drive shaft 4 Remove the lower section of the drive shaft which has the impellors fitted by using a spanner to loosen the shaft from the top end where there is a flattened section Once loosened this can be wound off the thread located a
15. for emptying and or cleaning in a sink 4 Any spillages of reagents especially acid or alkali must be wiped up immediately CARE NEEDED Cleaning of a recently used system Please note sterilization of the vessel and peripherals MUST be done by the user prior to your Starting work on the equipment 1 The pH and dissolved oxygen electrodes should be removed and stored in suitable reagents according to the manufacturer s instructions which come with each electrode Copies are included in the appendices of this operating manual 2 Periodic cleaning and regeneration of the electrodes are also covered by these instructions 3 The vessel should be rinsed several times in distilled water to remove any loose culture residues 4 Growth of culture on the vessel walls may require disassembly and light brushing to completely clean the glass 5 If the vessel must be tipped up to empty out any culture or cleaning liquid ensure both the glass section AND the metal support frame are BOTH held firmly This prevents the glass side arms of the vessel jacket being broken off by contact with the support frame Examination of the vessel during cleaning During cleaning of the vessel an examination for any chips or cracks in the vessel glass can be made and faulty vessel glasses MUST be replaced To disassemble the vessel glass from the support frame it is necessary to first loosen and then remove the bolts holding both the top an
16. on neck at right 2 RS232 serial port for printer computer connections on neck at left The mains socket main fuse the hour counter ID plate and motor connection cable are at the left rear of the base unit Top Section Service Manual Version 1 0 for LABFORS Page 25 LABFORS SERVICE MANUAL 6 3 Exchange of Pt 100 Sensor This is necessary when it is shown that an incorrect value is coming from the sensor Use a second calibrated probe in its place to confirm the fault is not with the cable or amplifier module If you do not have a second probe check the calibration values at 0oC eg ice water and approximately 50 C hand hot is considered to be 56 C Of course this only detects gross errors and not small inaccuracies If it can be shown the sensor is at fault exchange is simple 1 Locate Pt 100 sensor on top plate From the top a 2 pin Lemo connector can be seen in a 10mm diameter port 2 Undo the two slot head screws holding the sensor in the top plate 3 Pull firmly upwards to release the sensor from the port 4 Lightly grease the O ring on the replacement sensor with a little high vacuum grease 5 Replace the electrode firmly into the top plate port and screw down into place 6 Connect to the operational amplifier unit and check readings 6 4 Exchange of the Circulation Pump if fitted N B For systems with a heater pad the Aqua print connects directly to a socket on the left side of th
17. so this must be changed Check for bubbles in culture Check service supply Check as in 5 2 Replace if necessary Check connections Replace if necessary Check connections Check services supply of gas Check solenoid valve operation listen for activation does valve get warm over time test for flow with air Replace valve if necessary Adjust esp P term default values available Full 3 term control is often needed for good pO2 control Service Manual Version 1 0 for LABFORS Page 21 LABFORS SERVICE MANUAL 5 6 Antifoam amp Feed Pump Foam not sensed Foam always frequently detected Antifoam control does not work Feed Pump does not work see above for range of possible pump faults Service Manual Version 1 0 for LABFORS Possible Causes Faulty Antifoam electrode Check lead amp amplifier by shorting cables together does display show AF ON and or pump operates If YES If NO Processor fault Antifoam probe sheathing damaged Pump switched to ON Processor fault Control not active Pumps not set to Auto No reagent or inadequate Pump not operating properly If pump heads do not rotate Faulty pump motor Wrong tubing type Faulty Solenoid Is only one pump effected If YES Control output not given short out probe Check indicator LED s does the antifoam pump lights ON If NO Processor Fault Check Fermenter Overview does this show antifoam pump active If
18. the display asks for memory card to be inserted If YES Processor Problem Memory card problem Processor problem or fault with keypad Remedy Plug in switch mains ON Check plug fuse 10A Check fuse in base unit 10A Check plug connections Test mains cable continuity Check connections at mains socket in base unit Check reset in plug socket Touch any key LCD screen should now light Check power supply to 24V feed from transformer Replace transformer if necessary Replace back light Check 24V feed to main pcb Replace screen panel Replace main pcb Memory card should be inserted N B Never insert or remove a memory card if the unit is switched on Switch On and OFF to clear fault If no change remove amp replace processor or exchange it for a new one Re initialise card remove battery briefly to clear mem Switch On and Off If no change replace keypad or processor try processor first For general random faults with operation pumps etc check earthing amp labdrive pcb If setpoint calibration etc data is not being retained exchange memory card battery Service Manual Version 1 0 for LABFORS Page 17 LABFORS SERVICE MANUAL 5 2 Drive System Motor does not start Drive motor runs slowly or erratically pulses or hunts Possible Causes Speed controller OFF s the speed control switched ON at Start Fermenter or Fermenter Options screens PO2 control sw
19. E MANUAL CALIBRATION al pO E Z 11 12 13 Calibration of parameters is possible for both active and closed down fermenters The RPM parameter cannot be calibrated as this is factory set Press Menu to get to Main menu 2 to select parameter menu eg 1 to select the fermenter 1 6 eg 2 to select the desired parameter 1 5 2 to select the Calibrate screen Enter the High Ref values using the numeric keys eg 600C and press Enter to confirm Press DOWN ARROW to move on The High Read is a value generated by the A D converter in the instrumentation press Enter to allow the reading shown in the Act Read display to replace the current value in the High Read position Use the DOWN ARROW key to pass over the value Repeat the process for the Low Value and Low Reading Press ENTER to confirm or Menu PUMP FLOW CONTROL This screen allows the built in reagent and feed pumps to be set to ON OFF or AUTO control Acid Base and Feed pumps can be ON OFF or AUTO eg when the acid and base pumps are under the control of the pH control system The feed pump can only be set to ON or OFF The cycle and set values are in seconds and allow different flow rates to be achieved by varying the time a pump is active over time The maximum duty cycle for any pump is 40 of the total cycle time eg cycle 60 set 24 secs This screen is reached via the Fermenter Options Menu Use the key to toggle On O
20. LABFORS SERVICE MANUAL Labfor Service Manual Fermenters and Shakers For over 30 years INFORS AG ISO 9001 A Display B Keypad C RS232 Port D Memory Card Slot E Peristaltic Pumps F Pump ON Manual Button G Pt 100 Electrode Cable H pH Electrode Cable l Oxygen Electrode Cable J Antifoam Probe Cable K Baffles F QS Verk Handbuch Ser hb LabSer e doc L Air Inlet M Operational Amplifier N Rotameter O Water Pipe for Gas Cooler P Water Valve for Gas Cooler Q Base unit Heater Control R Water Inlet S Cooling Water Inlet Valve T Connector for Vessel Jacket U S Steel Tray for Vessel Service Manual Version 1 0 for LABFORS V Drive Motor W Port Fittings X Top Plate Clamp Y Mechanical Seal Z Drive Shaft 1 Top Plate 2 Overflow Pipe to Drain 3 Vessel Jacket Impellors 5 Sparger Page 1 LABFORS SERVICE MANUAL LABFORS SERVICE MANUAL Version 1 0 TABLE OF CONTENTS 1 Dimensions technical data and services requirements 2 Installation amp basic operation 3 Cleaning 4 Exchange of consumable parts 4 1 Vessel seal 4 2 O rings on port fittings amp clamping ports 4 3 Membranes filters reagent bottle fittings sample device 4 4 Mechanical seal removal of drive shaft amp impellors included 4 5 Battery on memory card 5 Troubleshooting guides 5 1 Basic Operation 5 2 Drive System 5 3 Temperature System 5 4 pH System 55 Dissolved oxygen system 5 6 Antifoam amp Feed Pump
21. NO Pump not switched to ON No set value given Remedy Check connections Replace antifoam probe Replace amplifier module Check connections Replace Replace sheathing Switch pump to Auto Check connections Replace Ensure control is turned ON Check and set to Auto if necessary pumps submenu Ensure bottles full right reagent amp connected Line open to vessel Check operation manually and prime pump Check connections Check drive belt Replace pump motor Check tubing type not too hard and spring tension Check connections to solenoid Replace solenoid Check connections Replace Labdrive pcb in 3rd section also for pump mal functions Check connections Replace processor or pcb Switch pump ON Give cycle amp set times Secs Page 22 LABFORS SERVICE MANUAL 5 7 IRIS Software Connections to Labfors assumes prior correct installation by Infors No Data logging or control Error opening com port Message box appears Logging but no Control No change in logged value Cable not connected Cable connected to wrong COM port Cable connected to wrong fermenter Cable not earthed at one end Fermenter not switched to remote operation COM port settings incorrect Fermenter mis assigned Device model incorrect eg set for ISF not S DDC Wrong protocol for comms Eg baud rate or parity mis set Conflict with other devices on COM port eg a mouse COM port settings incorre
22. NOTHER UNIT OF THE SAME TYPE IF POSSIBLE Service Manual Version 1 0 for LABFORS pH and Dissolved Oxygen Simulator Calibration is best achieved using a certified simulator for pH and dissolved oxygen as this tests both the cable and amplifier a suitable unit is made by the Valley Instrument Co Inc of Exton PA USA If calibration is successful using the simulator incorrect readings can then almost certainly be attributed to a faulty probe or earthing problem A simulator can be used for temperature also but it is often cheaper and easier to carry a spare Pt 100 sensor known to be calibrated properly ice water and boiling water provide 2 certain reference points at normal altitudes and atmospheric pressures If you do not have access to a simulator useful tests can still be performed on site LED RELATES TO THE INDIC ACTION OF ATOR Motor Foam Pump Base Pump Acid Pump Circ Pump Feed Pump Cooling Valve Heater Overtemp always ON 24V Power always ON Indicator LEDS in the 3rd section of the Labfors Page 24 LABFORS SERVICE MANUAL 6 2 Removal of Covers Main Sections of the Labfors The base unit of all Labfors contains the following Bottom Section Undo the 3 screws at each side to the rear of the panel and lift forward gently to gain access to Thermocirculator pump if fitted Heater element in pocket if fitted Cold water inlet pressure regulation valve Cold water control valve
23. ORS if installation is requested by the customer This is aided greatly when all services outlets include the necessary pressure reduction system already installed by the customer Water Inlet Cold amp preferably soft lt 50ppm suspended solids water supplied at a pressure of gt 1 5 bar enters from the left side of the base unit of the fermenter Push on connector pipes are supplied separately in a bag Push one of these into place and fit braided tubing to the cold water tap Use jubilee clips to secure pipe to fitting at both ends Water Drain The drain outlet is on the top of the fermenter jacket if applicable It requires a clear fall to the drain sink without sharp bends or kinks Push fit onto glass connection arm at the top of the vessel jacket CARE not applicable to systems which use an electrical heater pad Exit Air The exit air leaves via the disposable filter attached to the exit gas cooler if fitted If oxygen supplementation is used take care to pipe the exit air away safely to avoid explosion risk Service Manual Version 1 0 for LABFORS Page 7 LABFORS SERVICE MANUAL Vessel connections Temperature control Connect either the water circulation pipes to the vessel jacket using the rapid coupling connectors or plug in the heater had and secure to the vessel using the velcro tabs Inlet of water into the jacket is controlled by a manual valve on the right front side of the bottom se
24. and re assemble in the reverse order ED Right hand end showing main roller amp bar for pump head supports Dis assembly of the pump head section 1 Remove the circlip on the brass coloured rod which holds the pump assemblies in place 2 Withdraw the rod from the right side to release individual pump head holders 3 Remove the relevant pump head by taking away the retaining circlip Replace as necessary Reverse the procedure for re assembly GEE Main roller removed bar holding pump head supports can be seen Service Manual Version 1 0 for LABFORS Page 33 LABFORS SERVICE MANUAL 6 13 Removal of Back Cover amp Main pcb Modification Replacement of Master Units 1 Turn the Labfors around for access to the large black panel at the rear of the Master unit 2 Remove the memory card from slot on your left The card is replaced with the text towards you still looking at it from the back of the unit 3 Remove the screws along the side edges of the panel 4 left 4 right using a flat head screwdriver 4 Lift out inner the cover and turn it gently to the left You will see and earth lead at the bottom of the inside of the casing which needs removing with a cross head screwdriver 5 You now have in situ access to the main pcb The EPROM and microprocessor can be upgraded from here without further disassembly Replacement of EPROM 1 The EPROM gives the Labfors its identity It made need upgrad
25. at the front of the section and replaced There are both dip switches and potentiometers on the front edge of the pcb These alter the characteristics and response of the motor controller The DIP switches should be in the following positions The three potentiometers are usually factory set and should only be adjusted on advice from Infors Their purpose is P1 Internal set point value P2 Peak power limit P3 Constant power limit Location of dip switches and potentiometers speed control card partially withdrawn Service Manual Version 1 0 for LABFORS Page 29 LABFORS SERVICE MANUAL 6 9 Exchanging the rotameter inner cylinder This is a simple job usually required if a user acquires a large eg 13L vessel The maximum airflow needed is 1 5 vessel volumes per minute If this cannot be met by the original rotameter flow rate range a new one must be installed Calibration tables are included for each rotameter type in the Labfors operating manual The scale on the Vogtlin rotameters is calibrated in litres hr as standard and the type is given by the code after the manufacturer s name eg V100 80 14 is usual To exchange the inner cylinder which is the actual variable area tube 1 Remove the front plastic cover 4 screws 2 Unscrew the large screw on top using a hexagon key The tube can be levered out gently from between the top and bottom of the casing 3 Replace the tube with the new one
26. ck connections Check drive belt Replace pump motor Check tubing type not too hard and spring tension Check connections to solenoid Replace solenoid Check connections Replace Labdrive pcb in 3rd section also for pump mal functions Check connections Replace processor or pcb Adjust esp P term default values available Check reagents 0 1 1 0M Page 20 LABFORS SERVICE MANUAL 5 5 Dissolved oxygen system Possible Causes Faulty pO2 electrode No display or incorrect display of dissolved oxygen Faulty amplifier module Processor problem Control not active Actuator not switched to ON No gas flow into vessel Fault with Actuator a Stirrer Motor No dissolved oxygen control b Mass flow control valve c Gas Mix unit Dissolved oxygen drifts up or down over time or stirrer hunts PID settings incorrect Remedy Check connections Test calibration with zeroing gel and max speed air flow Use simulator if available Check slope of electrode for signs of ageing Replace membrane or whole electrode Check connections Replace sensor or use simulator to test Replace amplifier module Switch OFF and ON again Replace processor Switch control ON In Po2 Def screen select stirrer flow and or gas mix as appropriate Other settings must be made for flow and gas mix to work only control by stirrer is available as standard factory default is for flow control to be set ON
27. ct COM port not existing on this computer Conflict with other devices on COM port eg a mouse Parameter not set for this Fermenter settings are not correct Backup value may be ON look in Text view table Service Manual Version 1 0 for LABFORS Remedy Connect firmly at both ends Check which COM port a fermenter is assigned to and swop if needed Swop Cable if necessary Ensure earth strap connected at the computer end to a case screw Select this option at the Fermenter instrumentation Check in Peripherals dialogue box Extras menu devices sub menu Check in Fermenter Properties dialogue box Click on Settings button in Peripherals dialogue box and check seitings re fermenter manual Ensure the fermenter COM port is not shared check Control Panel settings in Windows Check in Peripherals dialogue box Extras menu devices sub menu Try different com port Ensure the fermenter COM port is not shared check Control Panel settings in Windows Click remote operation ON in the Edit Measured Parameter dialogue Set accept remote on the fermenter control panel Click backup value OFF in the Edit Measured Parameters dialogue Page 23 LABFORS SERVICE MANUAL 6 Repair Items 6 1 Testing of Process Parameters setup calibration actuators etc The Labfors can be thought of as a single container for measurement and control using at least 5 different operational sub systems
28. ction of the base unit Open this valve fully for first operation after connection of the vessel Connect the inlet pipe of the exit gas cooler using the small rapid coupling connector and connect braided tubing to the outlet top pipe and place the free end in the drain sink A second manual valve on the right front side of the fermenter controls water inlet into the exit gas cooler This is turned on to give a steady flow through the exit gas cooler The vessel top plate is equipped with a Pt 100 temperature probe This is in a 10mm clamped port and a 2 pin Lemo connector It is used for connecting to orange cable from the operational amplifier module Push to fit when the red spots on cable and connector are aligned Gas supply Fit a 0 22u filer to the top of the vessel sparger using braided tubing long enough to allow a clamp to be placed between the filter and the metal sparger pipe Connect the air line from the rotameter to the air sparger in the vessel by braided tubing pushing it onto the air filter If a gas mixing system is present connect the gas supply bottles to the appropriate inlets either to the rear of the base unit or on the separate gas mixing box Initial Setup Checklist Memory card in place Water line connected Drain line connected Air line connected Vessel connected to heating system Rotameter outlet connected to sparger Exit gas cooler inlet amp drain connected PT100 temperature sensor co
29. d bottom collars of the support frame together Any O ring seals which appear flattened or dry should be replaced and the new seals lightly greased with a mineral compound such as eg Edwards high vacuum grease Membranes which have been pierced should be replaced Service Manual Version 1 0 for LABFORS Page 12 LABFORS SERVICE MANUAL 4 Exchange of Consumable Parts During a routine service inspection a number of consumable parts may have to be exchanged 4 1 Vessel seal The vessel seal is critical for maintaining an uncontaminated environment for the culture 1 Remove the clamping ring from the vessel to release the top plate 2 Carefully lift the top plate upwards so the pipes etc clear the lip of the glass section and place the top plate gently on its side away from the glass section The glass section will continue to be supported by the metal frame surrounding it 3 The vessel seal is a large O ring sitting ina groove in the flange at the top of the glass section If it is flattened or shows signs of fraying then it should be replaced with a new one 4 The new seal is very lightly greased with silicone grease and placed in the groove It must fit snugly without any wrinkles If a good fit cannot be achieved discard the seal and use a new one 5 Re assemble the top plate onto the vessel glass and replace the clamp using even pressure on all the clamping screws finger tight only 4 2 O rings o
30. de of the housing Put the 4 small spacers into place and replace the screen pcb Re fit the perpendicular pcb and re connect the white wires from the screen Fit and tighten the retaining nuts to hold the screen pcb into place Reconnect the earth lead to the inside of the back cover plate of the screen keypad housing Pass cables though the holes in the back cover of the housing ready for reconnection to the main pcb Replace the nuts holding the back cover Re fit the screen keypad to the main pcb if this has been removed Reconnect the earth lead at the top right of the main pcb housing Replace the inner guide for the memory card Reconnect all cables Refit rear cover LCD Screen amp Light Buzzer pcb removed Screen Keypad re connected Service Manual Version 1 0 for LABFORS Page 36 LABFORS SERVICE MANUAL 7 1 Fitting an Analogue Box The Analogue Box allows additional input and output signals to be added to a Labfors Master unit It replaces satellite fermenter number 4 The box has connections at the front and rear Front hn From left hand edge looking at the front only the first three blocks of connectors are used on a basic analogue box remainder are for chart recorder outputs Block 1 Inputs 0 4 i e 5 analogue inputs 0 10V arranged in groups of two Block 2 Outputs 0 2 i e 3 analogue outputs 0 10V or 4 20ma arrranged in groups of 3 Block 3 Outputs 3 5 i e
31. dule Processor problem Control not active Control output not given Check indicator LED s is the heater light ON If NO Processor Fault Check Fermenter Overview does this show heater cooling valve active If NO Set a high setpoint eg 45 C Can you hear the circulation pump working If NO No Water Faulty Circulation pump if fitted If YES Does the vessel get hot If NO Over temp cut out fault Faulty heating element Faulty Aqua pcb Neg factor too high control biased towards cooling No Water or inadequate flow Set a low setpoint eg 5 C Can you hear the circulation pump working If NO Faulty Circulation pump if fitted If YES Does the vessel get hot If NO Faulty cooling valve Incorrect Neg Factor PID settings incorrect Remedy Check connections Test calibration with eg ice and hot water Replace amplifier module Check connections Replace Pt 100 and re test Switch OFF and ON again Replace processor Ensure control is turned ON Check connections Replace Labdrive pcb in 3rd section Check connections Replace processor or main pcb Turn water supply ON Check connections Replace circulation pump Check operation Check connections Replace heater Check power output to heater Replace pcb Adjust Turn cooling water ON Check connections Replace circulation pump Check connections Replace cooling valve Adjust eg if cooling water temperature is too high Adjust
32. e walled vessel 5 C under coolant temp to 60 70 C 500W per station Electronic brushless DC motor with tacho feedback PWM controlled Top drive with direct coupling via a single mechanical seal impellors amp baffles or a draft tube system OR Axial magnetic drive system approx 20 1200 rpm RS232 Tx Rx and ground only Memory card type PCMCIA 2 12pH 0 100 Total cycle time and dose time 40 max Total cycle time and dose time 40 max Optional conductivity probe 410 x 375 x 930 mm Master Base 375 x 375 x 735 mm Base only Electricity 230V 50Hz 6A noise free Water gt 1 5 bar Air 1 5 bar Service Manual Version 1 0 for LABFORS Page 4 LABFORS SERVICE MANUAL Overview of the Labfors Systems of Measurement amp Control TEMPERATURE Temperature is controlled using an electrical heating jacket or water PID Loop Temperature thermocirculator The thermocircualtor sends liquid around the vessel jacket and INPUT OUTPUT heats it with an electrical element if required Op Amp a Cooling water enters via a magnetic valve and leaves via an overflow pipe Water reee i goes either directly into the vessel jacket or into a cold finger depending on the Heater Cooling heating type nee The range of control is approximately 5 C above the cooling temperature to 60 C A signal from a Pt 100 temperature sensor in the culture is used for feedback control m by t
33. e base panel Withdraw Pt 100 The circulation pump provides heated water to the double jacket of the vessel for temperature control of the culture The pump switches on as soon as the temperature controller is turned on and can be heard when in operation Any air in the system which causes the noise is usually displaced quite quickly If the pump cannot be heard operating and the culture temperature alters very little this is an indication that the pump has failed Also the pump may have to be exchanged or at least repaired if it begins to leak Disassembly 1 Remove the screws supporting the bottom section of the base unit 3 at the rear left 3 rear right 2 Gently lift the bottom section forward and outwards a short way 3 Remove the earth leads 4 Reach inside and remove the small ribbon cable coming from the base unit onto the middle of the Aqua pcb part no20263 5 The front section can now be turned clear of the main body of the base unit to allow easy access 6 Locate the pump at the bottom of the section part 12325 7 Unsolder the red wire linked to the right angled tubing at the front edge of the pump 8 Disconnect the three wires supplying power to the pump from the terminal bock on the lower right hand edge of the Aqua pcb Note the arrangement of the wires 9 Disconnect from the water supply at each end of the Exchanging Circulation pump by unscrewing the Jubilee clips holding it to the Pump
34. e four screws 2 Left 2 Right holding the magnet solenoid housing to the pump head assembly 3 Turn the unit around so the pump heads face you 4 Remove the tensioning springs behind the pumps which link the pump heads to the solenoid magnets housing A pair of long nosed pliers are best for this Only the top part of the spring needs to be removed to separate the two parts 5 During re assembly ensure the plungers of the magnet solenoid are correctly re located relative to the metal bars at the bottom of the pump head section Correct operation depends on this Removal of a single magnet solenoid The magnet pull the pump heads towards the main roller after the drive motor has been activated 1 Undo the two cross head screws holding the selected magnet in place 2 Pull back the black insulation blanket at the rear of the magnet solenoid housing 3 De solder the two wires linked to the magnet 4 Replace magnet and solder connections into place Removal of main roller This part is rotated by the motor and provides the means for the peristaltic pump head to rotate when the relevant magnet solenoid pulls the pump head forward 1 Undo the circlip holding the main roller in place 2 Unscrew the retaining bolt using a hexagon key while holding the nut at the rear still with a spanner Remove the retaining bolt nut and two washers Pull out the main roller from the left hand end where the pulley is mounted 5 Refit
35. e module where the cables are connected is a blue potentiometer marked 1 9 When there is no foam the diode above should be lit To reduce sensitivity turn the potentiometer away from the 9 until the light goes out Foe ae Do For the foam sensor in the vessel ensure the black wire is connected to the vessel top plate and the red wire is connected to the probe itself r Rotameter Measurement module with operational amplifiers pcb Pd F Mi 4 id p Op Amplifier pcb ready for exchange Service Manual Version 1 0 for LABFORS Page 30 LABFORS SERVICE MANUAL 6 11 Exchange of the digital distribution pcb Labdrive This pcb carries signals from the instrumentation to the actuators eg heater valve pumps etc A set of indicator bars on the edge of the pcb shows when a particular actuator is supposed to be active If you do not see the correct light at the correct time then suspect a problem with this pcb Also a number of seemingly unrelated problems such as pumps chattering or the stirrer speed not working properly can sometimes be ascribed to faults in the Labdrive pcb part no 20271 Exchange is straightforward 1 Remove the front cover by undoing four screws 2 left 2 right 2 Remove the earth lead from the front panel 3 Remove the ribbon cable connector at the rear of the module and place the front section safely to one side 4 Remove the single screw holding the pcb at t
36. e to come out from the bottom of the instrument housing Replace the memory card the correct way around Connect the larger serial cable from the Serial Out 25 way D connector of the satellite unit to the 25 way D connector Serial IN on the Master unit Set the instrumentation to allow display of multiple fermenters See operating manual 7 3 Connecting a 3 1 gas mix system with Mass Flow Valve SECTION TO BE ADDED AT THIS POINT Position of Master analogue cable Free position oe Oe ee _ oe ain tos es Serial IN connection on Master base unit Rear view of gas mix unit showing mass flow control valve Service Manual Version 1 0 for LABFORS Page 38 LABFORS SERVICE MANUAL 7 4 Fitting a 2 Gas Mix System This provides an alternative control of pH and dissolved oxygen especially for supplementation and animal cell fermentations A kit is supplied containing A pcb holder with mounting for fitting to the inside of the main pcb housing s cover The screw needed for this is located ona black metal plate which is NOT used in this configuration A small pcb with indicator panel with the unused side plate Linked to the pcb holder is a connector which splits into a flat band cable with end 2 connections 25 way D and 20 way using a middle joining connector plus the leads connected to the gas valves marked O2 and CO2 which are electrically operated A small strip of insulating foam
37. ff or Auto options DOWN ARROW moves on to the next position Enter the cycle time in seconds and use Enter to confirm the new value DOWN ARROW moves you on Repeat for the Set time The flow setting allows the thermal mass flow valve if fitted to be controlled with a manual value for gas flow rate in litres per minute This valve is usually under the automatic control of the pO2 control system A final Enter or Menu confirms the new values Esc aborts the process DEF PO2 This screen allows several options to be set for control of dissolved oxygen It is accessed from the the Fermenter Menu If Flow Control is set to On using the key then oxygen control is via the optional thermal mass flow control valve If the RPM control is set to On then speed is used to control pO2 either in place of the flow valve or additional to it Max Influence sets a symmetrical limit in RPM in which control of pO2 can take place eg set point of 1000RPM with max influence of 200 would give control of pO2 by stirrer soeed from 1200 to 800 RPM only This is the only standard option Gas Mix and hand Mix only apply to systems with a special gas Mix unit Parameter Menu Fermenter F1 Parameter pH 1 Setpoint 2 Calibrate 3 PID Setup 4 Controller View 5 Calibrate all Select option Menu for Main Menu Enter or esc to Exit Fermenter gt F1 lt STATUS OK 00d00 00 Calibrate Fermenter F1 Parameter pH High Ref
38. h play to remove the drive belt from the motor pulley and the roller above Alternatively free the drive belt by temporarily unscrewing the pump module and removing the drive belt from the roller pulley 6 Disconnect the wires from the 8 way terminal block on the rear of the support plate noting the arrangement of the wires 7 Undo the nuts completely for removal of the pump motor 8 A single large nut at the rear holds the support plate if removal in its entirety is required 9 Re assembly is the reverse of this procedure u W J u k n F 2 Third section Labdrive pcb Pump motor face on showing retaining nuts Service Manual Version 1 0 for LABFORS Page 31 LABFORS SERVICE MANUAL b Exchange of pump heads roller and solenoid magnets ule 2 10 11 Remove the front cover of the third section by undoing four screws 2 left 2 right Remove the earth lead from the front panel of the third section Remove the ribbon cable connector at the rear of the module and place the front section safely to one side The pump motor and drive belt can be seen mounted on an internal plate to the left of the housing interior A large capacitor is also mounted on this plate below the motor Undo the 5 screws holding the top cover 1 back 3 rear right and 1 rear left Remove the screws above each pump head and pull the black pressure plates forwards Lift the cover carefully upwards then rearwards to exp
39. hange a setpoint From the Values Screen simply press Enter after first selecting the parameter with the cursor Alternatively press Menu to show the Main Menu and press 1 to select the Fermenter Menu Press 2 for the Parameter Menu The Parameter Selection sub menu will allow you to choose the parameter you want to change eg 1 RPM A new setpoint is entered using the numeric keys and confirmed with Enter DOWN ARROW moves to the Alarm Lim field where a symmetrical alarm limit value can be entered outside of which an alarm will be generated Press Enter to confirm the value and Enter again to return to the parameter menu 1 Fermenter Menue 2 Parameter Menue 3 Printer Comms 4 Hardware Options 5 Password 6 Copy Memory Card d Fermenter Menu Fermenter F 1 1 Start Stop 2 Parameter Menu 3 Fermenter 4 Pump Flow Settings 5 Edit Profile 6 Profile Control 7 Ferm Overview 8 Pump Timings 9 Alarms 0 pO2 Control def Fermenter gt Fl lt Status OK 00d00 00 Start Stop Fermenter F1 PH Control Off pO2 Control Off Temp Control Off RPM control Off Antifoam 2 Ont START FERMENTER Off Menu for Main Menu to change value Enter Esc to exit Fermenter gt F 1 gt Status OK 00d 20 49 Long View Name Setpoint Output 37 0 10 Flow Men for Main Menue Enter to Edit Setup Esc for Value Service Manual Version 1 0 for LABFORS Page 10 LABFORS SERVIC
40. he front bottom where it passes through the heat sink 5 Lift up slightly and pull forwards to release the pcb from the rear connector and its front support 6 Replace the pcb and reverse the procedure for assembly When you have the pcb removed it is possible to see the large capacitor at the very back of the third section 6 12 Exchange of peristaltic pump components The motor and drive belt for the peristaltic pumps can be accessed from the third section The single stepper motor drives a roller which operates any or all of the four peristaltic pumps on demand This is normally switched off unless pump action is called for or you press the manual button located behind the pump heads The top section houses the peristaltic pumps the solenoid magnets which pull the pump heads onto the roller and the manual switch for pump priming A small instrument fan part No 12343 is also located in the top section a Exchange of drive motor and belt 1 Remove the front cover by undoing four screws 2 left 2 right 2 Remove the earth lead from the front panel 3 Remove the ribbon cable connector at the rear of the module and place the front section safely to one side 4 The pump motor and drive belt can be seen mounted on an internal plate to the left of the housing interior A large capacitor is also mounted on this plate below the motor 5 The motor is held to the supporting plate by 4 nuts Loosen these nuts to give enoug
41. he main pcb 1 Remove back cover in the way described previously 2 Remove the ribbon connectors and small black cable from the screen keypad sections connecting to the main pcb 3 Note the orientation of the red line on the ribbon cables and number both the cables and the sockets for easy re location on the new pcb 4 Remove the green 24V power connector coming from the bottom of the base unit to the main pcb 5 Remove the serial link cable connected to the lower left of the base unit from the main pcb 6 Remove the analogue flat band cable connected to the lower right of the base unit from the main pcb 7 Remove the earth lead connected at the lower left of the pcb 8 Using a 7mm spanner undo the six nuts holding the pcb to the rear of the black housing 9 Remove the pcb part no 21697 complete Take care not to lose the small metal support spacers behind the pcb 10 Replacement is the reverse of these steps Removal of pcb Housing with Screen Keypad Unit 1 With the main pcb removed disconnect the earth leads at the top left of the black housing 2 Undo the four large screws holding the pcb housing in place the housing plus screen keypad come away as a single unit 3 Remove metal plates on bottom edge of the pcb housing for replacing in new housing Removal and Disassembly of Screen Keypad Main pcb removed 1 Place the screen keypad unit on a soft cloth to prevent removal of screen keypad
42. he micro processor to activate either the heater or the cooling valve A closed Pt 100 circulation of chilled water can be accommodated if required The main controller display shows actual value setpoint PID control etc STIRRER SPEED PO2 Valve E Eee A setpoint is given to the control circuitry by the PID Loop2 Speed microprocessor A tachometer in the motor provides An IN OUTPUT the H for Ca control Ue ie o eee Speed are tactory set for this parameter Ine main display PCB shows the actual value for speed The level of Motor dissolved oxygen can be cascaded to influence the stirrer speed Speed range is typically 20 800 or 40 1200 rpm Tala Adjustments can be made using potentiometers and Tacho dip switches on the speed controller pcb in the second f the base unit meter section o pH The pH measurement and control system consists PID Loop3 pH of an electrode linked to a signal conditioning module INPUT OUTPUT which sends the measured value to the microprocessor Op Amp a Calibration is digital and similar to the procedure for a bench meter Range is typically pH 2 12 kaa fee ae Integrated peristaltic pumps deliver a timed dose of either acid or alkali reagent into the fermenter vessel The total on time of each pump is recorded and can be used to meas
43. hen the al ii Zhe motor is above 250rpm View of motor connections to outlet of armoured cable Remove the connectors Remove plate holding the from the speed pcb 5 amp 6 armoured cable connection 8 Motor cables removed from base unit 9 Service Manual Version 1 0 for LABFORS Page 28 LABFORS SERVICE MANUAL 6 8 Exchange adjustment of the speed control pcb The speed control circuitry consists of the main pcb 22169 which has a smaller pcb 22168 piggybacked onto it If the motor does not work at all check the small fuse on the motor pcb first The small piggyback pcb is easily exchanged by unfastening the two connectors from the main speed control pcb and undoing the two screws holding the smaller pcb in place The main pcb is removed and replaced as follows 1 Unfasten slot head screws holding second section of drive system in place 2 x rear left 2x rear right 2 Carefully remove front section holding the gas pressure regulator 3 Remove the earth lead from the front section so it can be taken away 4 Withdraw the speed control pcb 22169 by pulling it forward free it from the 96 way connector it links to at the back of the second section 5 Disconnect the large white connector at the top rear of the speed pcb 6 Disconnect the brown power connector on the centre rear of the speed pcb 7 The speed control pcb can now be pulled forwards and freed from the retaining brackets
44. i e temperature speed pH dissolved oxygen and foam Additional functions can be added via peripheral items see Section 2 for explanations of standard functions Part of fault diagnosis and repair requires that you have a knowledge of how each process parameter is controlled and setup for normal operation This requires familiarisation with the section of the operating manual dealing with the U DDC instrumentation A short quide is included in Chapter 2 for testing on installation but more detailed information is required for fault diagnosis If the Labfors does not function at all on switching ON check the cable fuse laboratory earth trios and main fuse on the machine by the power indicator light A fuse is also present on the motor controller pcb printed circuit board Testing each parameter should follow a common pattern 1 Ensure relevant probe is in place look for signs of damage or eg no electrolyte or physical dents 2 Ensure correct services are switched ON eg water 3 Ensure the parameter has a realistic setpoint for the test eg to check the cooling valve the temperature setpoint may be set to 5 C 4 Check that control in Dead Band BD is switched to ON amp or the actual value for Deadband is near zero eg lt 0 5 5 Check calibration of parameter before starting fermenter 6 Check PID values for irregularities eg large value or no P value 7 Start fermenter and ensure the parameter is turned ON 8
45. if fitted Heater pcb 20263 with cut out if no water present Transformer connection block amp mains socket Water inlet pipe on left if fitted OR Electrical connections to heater pad if fitted Manual control valves for water inlet to vessel jacket if fitted and exit gas cooler Soe Sig et elias tN Second Section Undo the 2 screws at each side to the rear of the panel and lift forward gently to gain access to 1 Air pressure regulation valve 2 Rotameter fixing screws 3 Motor controller pcb with adjustment potentiometers p es 4 Mains transformer OF ilies P 5 Air inlet pipe on right of panel cover t pp N Third Section Undo the 2 screws at each side to the rear of the panel and lift forward gently to gain access to 1 Operational amplifier module amp ribbon cable 2 Diagnostic LED s showing actuator operation 3 Digital controller pcb 4 Motor for peristaltic pumps amp drive belt Top Section Undo the 3 screws on right side to the rear of the panel 1 screw on the left rear plus an additional screw at the top rear Remove the screws on the top of each peristaltic pump Lift the panel forwards then upwards past the pumps to reveal Peristaltic pump heads Roller Solenoid valves to pull pump heads onto roller Switch for manually starting pump motor Small instrument fan Shee le ae Main pcb Housing Screen and Keypad Master only From the front side you can gain access to 1 Memory card slot
46. ils Flowrate doc 8 4 Calibration graphs for rotameters Calibgraph doc 8 5 Where to find the Serial No SerialCont doc 8 6 Contact details for Infors SerialCont doc 8 7 Labfors Components What s Where Whatwhre doc 8 8 U DDC Instrumentation LABUDDC doc 8 9 Addendum to Labfors U DDC installation information UDDCinst doc 8 10 Addendum PID Control Overview PiDover doc 8 11 Trouble shooting contamination leaks VesCont doc 8 12 Labfors Outline diagnostic table Faults doc 8 13 Initial setup of electrodes ElecPrep doc 8 14 How to exchange the sheathing on an antifoam probe AFFROINS doc 8 15 Service Agreement Checks for annual preventive maintenance LabAnSer doc Service Manual Version 1 0 for LABFORS Page 3 LABFORS SERVICE MANUAL Working volume No of vessels No parameters No reagent pumps Temperature Control Temperature Range Heating Stirrer Speed range s Data connection Profile storage pH range pO range Antifoam Feed Pump Dimensions Length x Depth x Height Services 1 Dimensions Technical Data and Services Requirements Approx 0 5 to 10L Up to three base units per Master C rpm pH measure amp PID control pO measure amp PID control options antifoam substrate cycle dose control Optional mass flow control and gas mix 4 per base unit Water temperature control via a Double jacketed vessel OR Heater pad temperature control with a singl
47. ing it forward 5 Screw the new fitting holding the two valves carefully into place do NOT use the delicate arms of the valves themselves Refit the rotameter and replace the cover 6 Turn on the Labfors and set the unit for operation with the gas valves Display Mix ON Gas Mix ON Gas Mix AUTO Mix Offset0 001 Negative factor 0 in PID setup for pO2 see diagram N B the value of last setpoint will latch even if controller turned off CO2 valve works in parallel with Acid pump this MUST be set to Auto Gas valves fitted to rotameter Service Manual Version 1 0 for LABFORS Page 39 LABFORS SERVICE MANUAL 7 5 Linking to IRIS Software Troubleshooting Connection amp Set up IRIS NT software provides data logging graphical display archiving and supervisory set point control SSPC for Infors fermenters The software must be installed on a PC running Microsoft Windows 9x or NT4 2000 The link to the Labfors is made via an RS232 serial cable which connects directly to a port on the main pcb Control of the type of output and communications protocol is handled by special settings available in the Printer Comms menu option of the U DDC instrumentation Use of the Labfors with a printer can also be controlled from here Details of installation of the IRIS software and operation with the Labfors is given in the operating manual for IRIS NT This section deals with the very basic physical set up and simple troubleshooti
48. ing to eg add an analogue box or other additional facilities 2 The EPROM is located to the left of the large square microprocessor chip located in the top third of the pcb 3 Removal can be achieved using a chip extractor or by gentle leverage with a penknife blade or fine screwdriver CARE 4 Before placing the new EPROM in the empty socket check the pins are all properly aligned and if not gently press the side on the chip onto a hard surface to cause all the pins on that side to move inwards fractionally 5 Locate the EPROM correctly by lining up the notch cut out of one end of the EPROM with a corresponding notch on the socket Press firmly into place ensuring no pins are bent under during this process Replacement of the Microprocessor 1 This is not normally necessary unless a fault condition exists The microprocessor is easily located as it is a large square socketed chip in the top third of the pcb 2 Removal must be made using the correct extraction tool available from main electronics suppliers which has two claws that locate into slots in the socket 3 Note the position of two small circles on the chip diagonally opposite relative to these long slots in the socket so the new microprocessor can be correctly oriented Removal of Processor ATELE Service Manual Version 1 0 for LABFORS Hi Location of EPROM amp Processor Page 34 LABFORS SERVICE MANUAL Replacement of t
49. itched on and set to work with stirrer Stirrer setpoint 0 Drive shaft not moving Can the drive shaft on the vessel top plate be turned by hand If NO No power to motor Is power being supplied to motor pcb No power getting to motor from speed control pcb Speed control malfunction Does speed match displayed value for speed visual estimation lf NO Motor bearings failure Tachometer fault Check DIP switches are correctly set Service Manual Version 1 0 for LABFORS Remedy Switch ON Switch pO2 Control by Stirrer OFF to test operation Check Stirrer set point gt 0 and deadband does not include zero rpm Remove obstruction or replace bearings on drive shaft Replace speed control pcb or motor Check connections Check connections to speed controller pcb Check position of DIP switches and potentiometers on speed control pcb are set correctly Check motor bearings by rotating motor shaft by hand If PO2 control by stirrer is switched ON turn it OFF Remove top from motor and visually check tachometer segments for signs of damage Page 18 LABFORS SERVICE MANUAL 5 3 Temperature System No display or incorrect display of temperature No temperature control No heating or inadequate heating No cooling or inadequate cooling Temperature drifts up or down over time Service Manual Version 1 0 for LABFORS Possible Causes Faulty Pt 100 sensor Faulty amplifier mo
50. laced Autoclaving sterilizes the glycerine This needs only to be done occasionally but should be checked every time the vessel is to be used Connection to a computer Printer The Labfors has an RS232 serial port on the left side of the black support for the screen keypad If specified a cable should be provided which connects to this port and a 9 pin serial port at the rear of the computer Installation and use of IRIS software is the subject of another guide Printers must have a serial port or a parallel serial converter in order to link directly to the Labfors Additional cables and equipment lt may be that some extra cables are included in the packing These are likely to be for external analogue pumps satellite base units or special cables for connecting peripheral items eg turbidity meter methanol sensor etc Specific instructions for connecting these items should be available If in any doubt contact Infors Reagent bottles are normally provided with the system These are set up separately and for test purposes can be filled with water See diagram for correct set up Page 8 LABFORS SERVICE MANUAL Bringing into use from a service viewpoint 1 Check all O ring seals for wear and replace as necessary Smear with a little high temp grease 2 Undo the clamping ring using the screw at the front of the vessel The top plate can be lifted clear of the vessel glass which remains in place
51. le of the card in the Write position before replacing it in the Labfors The card will be initialised by the Labfors on first switching the fermenter on and all setpoint values set to zero with defaults for all other data If the customer has downloaded the current configuration to a PC the data can be restored to the card using Option 6 in the Main Menu Copy Memory Card Memory Card Screwdriver Screw Battery Write protect Holder tab Service Manual Version 1 0 for LABFORS Page 16 LABFORS SERVICE MANUAL 5 Trouble shooting the Labfors 5 1 Basic Operation Labfors does not work at all No green mains power light Mains power but no display LCD Screen halts at P O S T Screen Power On Self Test Screen shows random characters and or odd behaviour of pumps etc System hangs or does not respond to inputs Keys fail to work Possible Causes Power lead not plugged in or Mains power not turned ON Fuses blown Break in power connections Unit switched ON but not touched for longer than 30 minutes No 24V supply Can the red LED indicating 24V supply be seen in the LED display in the third section of the Labfors If NO No Back light Can figures be seen dimly in the LCD display lf YES No 24V supply to pcb Main pcb or back light faulty f 24V supply to main pcb OK can peristaltic pumps be operated manually f YES If NO No Memory card Do all tests show pass and
52. n port fittings amp Clamping Ports The O rings seal the port closures against the top plate so no microbes can get in or out If their effectiveness is compromised the fermenter is functionally useless The port closure O rings are located in grooves in the closure and can be easily levered out for exchange Remember to lightly grease the new O ring Port fittings which have pipes going through them e g sampling device antifoam probe use a clamping arrangement to secure the pipe in addition to the O ring which actually seals against the top plate In this case there will usually be a small cylinder of silicone tubing inside the fitting which may become deformed and sticky over time This should be replaced at the same time The antifoam electrode has an insulating sheath that fits over the length of the probe except for the tip This is like a transparent plastic coating which can become ripped in time Replacement involves removal of the old covering and applying the new cover using the same heat shrink method employed for electrical cable sheathing with a strong blower or a flame Service Manual Version 1 0 for LABFORS Page 13 LABFORS SERVICE MANUAL eninge Hi Reagent Bottle Fittings Reagent Bottle Parts Disassembled These additional items are usually replaced by the user as necessary However as part of routine maintenance it makes sense to check any fittings in these perpiheral items eg dip tubes
53. n to the plug fuse 2 fuses can be found behind a cover above the location of the connecting socket in the mains inlet and On Off switch Air The air inlet can be found on the left side of the fermenter Use only clean dry oil and dust free air with a pressure of gt 2 0 bar A push on Connector pipe is used with braided tubing Fit a jubilee clip at each end of the pipe to secure it Inlet air is supplied through the rotameter variable area flow meter to the vessel Ensure this is turned on using the black knob below the rotameter scale Calibration graphs are provided in the operating manual Packing and disposal The LABFORS is shipped in a wooden crate All packing materials are environmentally friendly and can be re cycled Unpacking and Checking Please make sure that no part of the LABFORS sustains damage during unpacking Use the delivery documentation to ensure that all parts are present If there is a discrepancy between the list and the contents and or there are any signs of transport damage please contact Infors at once If this advice is not followed promptly any resulting costs will be the responsibility of the purchaser All accessories and flexible connection pipes are usually packed in a cardboard box placed on top of the fermenter base unit The vessel is also packed in a separate cardboard box within the crate Services The final connection of the fermenter to the services will be made by INF
54. ng for an installed system you may find already connected Serial cable connections Troubleshooting for an installed system See chart in Section 5 8 Connection to a PC or a printer 10 20 30 30 6 70 850 9 50 RS 232 On the side of the unit a male 9 pin connector is provided Description Computer Side Computer Side SDDC pins Pin Configuration Pin 2 receive data RD Ground Pin 3 send data TD Tx Rx Pin 5 Signal ground Rx Tx No other connections are used by the LABFORS For a functional data connection the cable must match this schematic A cable 9 Pin female 9 Pin female is available from INFORS Should your serial connection be to a 25 pin socket a standard 9 25 pin adaptor can be used to allow a direct connection It is possible to make your cable connections according to the table and schematic shown Note With 9 pin to 9 pin connecting plugs the data lines 2 and 3 must be crossed If a printer is to be connected this must be via a SERIAL connection or a serial to parallel converter must be used lt needs no additional settings to be made Also when a terminal programme is used it is not necessary to issue a command for data to be transmitted Setup 1 Connect one end of the serial cable to the Labfors Master Connect the other end to the 9 pin serial port of the PC Com1 or Com 2 3 Connect the earth lead at one end to a good earth point eg a casing screw 4 Refe
55. nnected Drive motor connected 10 Air supply turned on 11 Water supply turned on 12 Mains power on oS oe Se This is purely for testing the basic functions of the fermenter Connecting of probes etc is covered in a separate section Service Manual Version 1 0 for LABFORS Drive System The drive motor is permanently connected to the right hand side of the base unit It is placed on top of the vessel and locks into place using a lug on the vessel drive hub and a key way on the connecting hub of the drive motor To fit place the motor on the vessel drive hub having first removed the plastic protection cap and turn until the motor falls into place with only light guidance Before first use the vessel drive hub needs lubrication if this is not already present as part of the factory testing of the vessel NEVER run the drive system dry it will ruin the mechanical seal in a very short time Lubrication of the top seal The base of the drive hub has two short pipes sticking up where it joins the vessel top plate They are joined by a short length of silicone tubing If liquid cannot be seen in this silicone tube the seal need re filling To do this undo the silicone tubing at the longer pipe and connect a syringe containing a few millilitres of glycerine Inject the glycerine gently until liquid comes out of the top of the longer steel pipe At this point the seal system is filled and the tubing can be rep
56. ose the pump system Disconnect the electrical wiring from the rear of the pump unit The white connector block behind the pump magnets can be pulled apart to break most of the connections but a red and brown wire will still need to be cut to totally free the unit Undo the two large bolts left and right holding the whole pump module in place with a 6mm hexagon key Pull module forward and down slightly to remove the drive belt from the pulley on the left side of the roller The whole peristaltic pump module with solenoid magnets can now be placed on the bench for further disassembly Side view showing right retaining bolt 9 Magnet solenoids showing wiring behind 8 Close up showing left retaining bolt amp roller 9 Service Manual Version 1 0 for LABFORS Page 32 LABFORS SERVICE MANUAL c Dis assembly of pump module after removal from Labfors casing N B Replacement of the main roller or pump heads should not be necessary unless they are physically damaged Important Replacement of the rubber bands around the edge of each pump head to provide grip on the peristaltic tubing does not require dis assembly of the pump module for replacement They can be pulled off the relevant pump head and replaced Removal of screws holding pump while the whole unit in place heads and magnets 2 Separation of pump heads from the magnet solenoids 1 Turn pump module so the pumps face away from you Remove th
57. r to IRIS manual for operation from this point Service Manual Version 1 0 for LABFORS Page 40
58. rol system antifoam pump used in reverse to withdraw culture or a simple side arm acting as an overflow option Even if the fermenter is operated as a chemosiat for a short time large containers are needed for both fresh medium and harvest Antifoam reagent can be added to the fresh medium at a low concentration eg 1 20 000 if required Service Manual Version 1 0 for LABFORS Page 6 LABFORS SERVICE MANUAL 2 Setup and Basic Operation Transport n view of the weight of the complete LABFORS and packing materials it should never be carried by one person alone For moving the packed system over long distances a low trolley or palette raiser is recommended If the LABFORS is to be transported by a fork lift truck it is vital it is secured in such a way that there is no chance of it falling off or otherwise being damaged Location The LABFORS fermenter is best mounted on a laboratory bench of suitable working height Access to air water and power services should be nearby along with a suitable drain sink A free space of 50cm should be left all around the fermenter to make servicing both easier and quicker The unit should be sited away from potential sources of electrical noise Services Connections Power 230V 10 A single phase supply for standard LABFORS The power supply should be clean and constant if not it is recommend fitting of suitable filters and or a UPS to the mains power supply In additio
59. s switch The motor may need replacing due to physical damage no feedback from the internal tacho generator in the top unit bearings failure or the coil windings burning out The motor is controlled by a speed controller pcb which is mounted in the second section of the base unit Connections to the motor are all made using plug in connectors 1 Unfasten slot head screws holding second section of drive system in place 2 x rear left 2x rear right 2 Carefully remove front section holding the gas pressure regulator 3 Remove the earth lead from the front section so it can be taken away 4 Withdraw the speed control pcb 22169 by pulling it forward to reveal the connections to the motor at the rear of the speed pcb 5 Disconnect the large white connector at the top rear of the speed pcb 6 Disconnect the power connector on the centre rear of the speed pcb 7 Disconnect the free hanging terminal block with connections from the motor black red yellow wires L R This is in the main bundle of wiring at the back of this section 8 Undo the rectangular armoured cable connecting panel to the outside rear right side of the base unit 2 screws 9 Pass the connectors out to free the motor and cabling 10 Reverse stages 8 1 above to connect the new moior which is supplied with the armoured cable and metal elbow already fitted 11 Check new motor for speed control on 3 12 A green LED will light on the speed control pcb w
60. t is pushed over the drive shaft and located snugly into the O ring The seal is shaped for this with a step which fits into the O ring The seal is handled by its SIDES ONLY during this operation 11 The metal rotating seal is now pushed into place so the polished faces meet securely A small O ring and washer which are inside this part of the seal may come loose during fitting and must be pushed back into place firmly 12 The top of the rotating seal has a slot in it into which a corresponding lug on the tensioning spring is fitted by pushing the spring the right way around onto the shaft until the lug firmly locates 13 The retaining ring can be slipped over the drive shaft and pushed down firmly to hold the spring in place 14 Pushing down firmly on the retaining collar compress the spring until the gap between the flat base of the top plate and the bottom edge of the retaining ring is 17mm see diagram 15 Still holding the tension on the spring tighten the retaining collar grub screw with an allen key until it is secure The lower section of the drive shaft can now be screwed back and tightened WARNIING The lower section of the drive shaft can be removed for exchange or cleaning but the retaining ring should never be loosened unless you intend to replace the mechanical seal This may dislodge the seal face and cause scoring Mechanical Seal Component Parts Washer Hard face Soft Face Spring O ring Hard
61. t the top of the remaining part of the shaft which is normally h f the i 5 S ed by the body of the lower drive Retaihing Lockitg Spring Ring 6 A small Allen key is used to loosen the retaining ring grub screw which holds the Drive shaft Static seal tensioning spring onto the drive shaft and in top plate both can then be withdrawn by pulling firmly upwards so they come free 7 The rotating metal part of the mechanical seal can now be removed by pulling upwards to expose the soft face of the static carbon slip ring Any signs of scoring or rings on this face is a clear indicator of wear Service Manual Version 1 0 for LABFORS Page 14 LABFORS SERVICE MANUAL 8 The static part of the seal can be lifted out by prizing it upwards from the recess which retains it An O ring is located beneath the static seal which must also be removed A new seal is fitted by reversing this procedure WARNING Do not touch either face of the static or rotating parts of the mechanical seal as this can damage them The new seal is packed assembled but there are covers over the matching faces of the static and rotating seals which must be removed when they are ready to be fitted 9 The O ring is firstly greased lightly with eg high vacuum grease and placed into the recess of the drive shaft bearing housing by carefully sliding it over the end of the drive shaft 10 The cover is removed from the soft static seal and i
62. ting manual Service Manual Version 1 0 for LABFORS Page 37 LABFORS SERVICE MANUAL 7 2 Fitting a Satellite unit to a Labfors Master This upgrade is fairly quick and easy The satellite unit has a base unit and a vessel but no instrumentation It has an independent power supply Up to two additional satellite units can be added to the Labfors Master unit even if an analogue box is already fitted be 2 We Set up the satellite unit close within 3 metres maximum of the Master unit Turn off the Master unit and ensure the satellite also is not powered Turn the Labfors Master unit around for access to the large black panel at the rear of the Master unit Remove the memory card from slot on your left The card is replaced with the text towards you still looking at it from the back of the unit Remove the screws along the side edges of the panel 4 left 4 right using a flat head screwdriver Lift out inner the cover and turn it gently to the left You will see and earth lead at the bottom of the inside of the casing which needs removing with a cross head screwdriver The small flat band cable from the Analogue D socket at the rear of the satellite unit is placed in either socket 2 or 3 which is on the upper right side of the main pcb For location an analogue box if fitted is always in position 4 and the equivalent cable from the master base unit will be in position 1 Replace the rear cover allowing the new cabl
63. ting the arrangement of the wires 8 Remove the circlip part 11597 holding the heater cartridge from its sheath using a pair of circlip pliers 9 Withdraw the faulty heater element 10 Grease the O ring 12969 on the new heater element and place into the sheath 11 Connect wiring to Aqua pcb 6 6 replacement of the Aqua small heater pcb This part would need to be exchanged if power was not being supplied to either the circulation pump or the heater 1 Remove wiring from the right hand side of the terminal block from the heater element pump and feed from the base unit Note the arrangement of the wires 2 Remove wiring from the left side of the terminal block from the various over temperature devices 3 The Aqua pcb is held to the front casing by 4 nuts undo these to free the pcb for replacement Other small electrical components can be exchanged The 2 way magnetic cooling valve 19948 Temperature switch for 900C cut out braided 12271 Temperature switch 800C complete 21989 Mains power inlet socket and filter 14499 Fuse 10A 12292 Mains ON light and green cover 12250 19162 Operational hour counter 12573 econ oa Service Manual Version 1 0 for LABFORS Page 27 LABFORS SERVICE MANUAL 6 7 Exchange of the Drive Motor The most usual form of Labfors motor is the current round bodied unit It is connected to the base unit via an armoured cable terminating on the right side above the main
64. trodes are too long to fit without The other vessel fittings can be added such as sample pipe exit gas cooler and acid alkali inlets Retaining screw fo SIDE VIEW RAT colar cee OPERATION 1 Air flow should be set to no more than 1 5 vessel volumes per minute eg for a 1 2L E ag working volume vessel the max is 1 8NL min ee _ Purp alin oy TO VESSEL 2 Pass the tubing from the reagent bottles eee fi through the appropriate peristaltic pump b ae using the small connector in these lines to a ee ene stop the tubing from being pulled through the bias pie pump Unclamp the connection between the bottle and the vessel 3 Calibrate the 100 dissolved oxygen high value by passing air through the vessel at maximum flow rate when the stirrer speed is also at the maximum to be used At least 2 6 Connect hours is needed before electrode is usable tubing 4 Complete the set up by setting appropriate Calibrate setpoint values This should be sufficient for O2 100 an installation check without autoclaving Service Manual Version 1 0 for LABFORS Page 9 LABFORS SERVICE MANUAL UDDC Instrumentation basic points The UDDC2 instrumentation is based on a powerful industrial process controller which uses a memory card type PCMCIA to store data about Set points profiles etc The keypad is splashproof and has several sections each with keys which perform specific tasks
65. ure how much reagent has been added Temperature compensation can be activated if required A gas valve for COz may replace the acid pump DISSOLVED OXYGEN Measurement of dissolved oxygen is via an electrode PID Loop4 pO2 linked to an integrated operational amplifier within the signal conditioning module A polarising voltage is also INPUT OUTPUT1 OUTPUT2 supplied by this unit Calibration is similar to a bench fama meter with zero and 100 values being set Motor i Thema Control is by a PID loop acting on either the stirrer speed or by influencing an optional thermal mass flow control valve to adjust the air flow rate A gas mixing unit can be used for special applications such as animal cell culture Range 0 100 Service Manual Version 1 0 for LABFORS Page 5 LABFORS SERVICE MANUAL ANTIFOAM optional Control of foam is based on a conductivity PID LoopS Foam INPUT Probe Op Amp OUTPUT Antifoam Pump probe above the culture surface detecting the build up of foam by contact with the probe tip The rest of the probe is insulated to prevent false signals After a user selectable waiting time delay the integral peristaltic pump delivers a dose of antifoam reagent shot The system then resets and will only add more reagent if foam is still present This arrangement can double as a level control system to remove culture GAS FLOW Gas

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