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1. 0 50 100 150 200 250 300 fL Figure 4 2 3 Ahistogram that does not show differential result because the WBC result is less than 0 5 0 50 100 150 200 250 300 fL WBC 0 4 x 1097 Figure 4 3 4 Ahistogram that does not show differential result because the peak is in the middle of the histogram 0 50 100 150 200 250 300 fL Figure 4 4 5 A histogram that does not show differential result because of increased nucleated erythrocytes or interference or inadequate hemolysis BC 2800 Auto Hematology Analyzer V1 1 4 1 Histograms and Pulse Graphs 0 50 100 150 200 250 300 fL Figure 4 5 6 A histogram that does not show differential result because of severe interference check the pulse graph to determine whether there is interference in the WBC channel 0 50 100 150 200 250 300 fL Figure 4 6 7 No lyse reagent or poor hemolysis 0 50 100 150 200 250 300 fL Figure 4 7 8 Increased granulocytes WBC 0 50 100 150 200 250 200 fL Figure 4 8 9 Increased lymphocytes WBC R2 O 50 100 150 200 250 300 fL Figure 4 9 10 Tumor patient WBC R4 _ O 50 100 150 200 250 300fL Figure 4 10 11 Increased monocytes WBC 7 Ss Seed Sn 0 50 100 150 200 250 300 fL Figure 4 11 4 2 BC 2800 Auto Hematology Analyzer V1 1 Histograms and Pulse Graphs 4 2 Pulse Graphs After each count the system can save the original sampling pulses of this time We can
2. Figure 6 15 A dialog box of Prepare to ship Move the cursor to Yes and press ENTER if you want to proceed with this procedure or to No and press ENTER if you wan to abort this operation If there is any error present the system will not perform this procedure Follow the instructions displayed on the screen to remove the error before trying to perform the procedure again After draining the tubing follow the instructions displayed on the screen to put the rinse diluent and lyse tubing into distilled water and press ENTER to flush this analyzer with the distilled water When the washing is over follow the instructions displayed on the screen to remove the rinse diluent and lyse tubing from the distilled water and press ENTER to drain the tubing again Turn off the analyzer when the screen displays Turn off the analyzer Wipe this analyzer dry and wrap it up for storage 6 16 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 3 System Status The items displayed in the Status screen reflect how the system is functioning and contribute significantly to diagnosing system errors You may follow the instructions given below to check those items Press MENU to enter the system menu and press the appropriate arrow keys to move the cursor to Service Status as Figure 6 16 shows Current sample WBC 3 1 x 10 L Lymph 3 7 x 10 L Mi ct 0 7 x 10 7L Wh ID 3118 Grane 47x tol BL Time 01 24
3. Figure 6 4 Rinse Priming screen 6 6 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 2 3 Lyse Prime Biohazard iS Wear standard laboratory attire including rubber gloves and D4 follow safe laboratory procedures when handling any material in the laboratory You should perform the Lyse prime procedure to prime the lyse tubing with lyse when there are bubbles in the tubing or the lyse in the tubing is contaminated or the old lyse ran out and a new container of lyse is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys t J lt to move the cursor to Lyse prime Press ENTER to prime the tubing with lyse and the priming progress will be displayed at the bottom of the screen as Figure 6 5 shows When the priming is done the screen will return to the initial state Dilvent prime Lyse test Rinse prime Clean bath Drain bath Zap aperture Drain tubing Flush aperture Clean wipe black Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming lyse Cyse prime Select service Figure 6 5 Lyse priming screen 6 2 4 Zap Aperture You can perform the Zap aperture procedure to unclog or prevent clogging Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys t lt BC 2800 Auto Hematology Analyzer V1 1 6
4. System Self Test eneee e eieiei 6 18 o oe E E EEPE PE EE PEPEE 6 23 6 6 System Configuration 2000000000 0000000000 00000000 01000000 0t000eeeeteeereeeteeeneneeee 6 25 6 7 Printing Management 2 24 2 02 1552 5 00ic0ck oon e eee ea 6 26 6 8 Adjusting Sample Probe and Replacing Probe Wipe 08 6 28 Chapter7 TroubleSMOoting sic 2c oct 2c nce acces at eds ad cec tad eSee sad ceded aed aedecels 7 1 GM Eror Godes ieee e eee e eE e ded eucde baa E E EERE AEE EEE 7 2 AZ SOMOS Alain natal au a E Me sede E T T 7 3 Chapters List of Spare parts sic cael ccidvost ce didtesannne aectielstacatestacelsiees 8 1 Appendix BC 2800 Error MeSSaQ ccccccccccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeseeaes 1 BC 2800 Auto Hematology Analyzer V 1 1 Hardware Chapter1 Hardware 1 1 General 1 1 1 Schematic puesane shesnneneesnsa _ Analog board Power drive K keyboard hye Se pannes i g ig i oe Figure 1 1 Schematic of the CPU board The CPU FPGA and Super I O are the major components on the board The CPU carries out the instructions and functions as the core of the board The FPGA functions as the relay between the CPU and the Super IO The Super I O includes various interfaces that can be accessed by the CPU through the FPGA System memories are SDRAMs The DOM is a Disk On Module that stores the system software and test data The RTC is a real time clock System confi
5. Be sure not to touch the power plug at the back of this analyzer with wet hands or wet rags Be sure not use organic solvent or acid alkaline detergent to wash the surface of this analyzer Otherwise the surface may fade or become corrupted Be sure to avoid direct contact with the reagents that will hurt your eyes skin and diaphragm In case you spill the reagents on you skin be sure to wash them off with much water In case you spill the reagents into your eyes be sure to immediately wash your eyes with much water and go see a doctor for further treatment 6 2 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 1 2 Recommended Regular Maintenance Table 6 1 Recommended Regular Maintenance Maintenance Period Content of Maintenance Everyday If you are to use this analyzer 24 hours a day be sure to perform the E Z cleanser cleaning procedure everyday Run the QC program everyday S Every three days If you are to use this analyzer 24 hours a day be sure to perform the Probe cleanser cleaning procedure every three days Every Week If you shut down your analyzer every day and follow the specified shutdown procedure to do that you need to perform the Probe cleanser cleaning procedure every week Every Month You should use the supplied probe localizer to calibrate the position of the probe to that of the wipe block The analysis result is sensitive to their alignment As
6. amp Time and set the time and date and restart the analyzer to enable the new settings If the error still remains call BC 2800 Auto Hematology Analyzer V1 1 7 7 Troubleshooting Mindray Customer Service Department or the distributor 7 2 24 Syringe Motor Error Something is wrong with the motor controls the syringe that aspirates dispenses samples and reagents Solution Access Service Self test Machine and test the motor The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor 7 2 25 Rotation Motor Error Something is wrong with the motor that rotates the sample probe Access Service Self test Machine and test the motor The error will be cleared if the result is normal Otherwise call Mindray Customer Service Department or the distributor 7 2 26 Elevator Motor Error Something is wrong with the motor that controls elevation of the sample probe Access Service Self test Machine and test the motor The error will be cleared if the result is normal Otherwise call Mindray Customer Service Department or the distributor 7 2 27 A D Error Something is wrong with the A D converter on the CPU board Solution Access Service Self test Circuit Test the A D interrupt The error will be cleared if the test result is normal Otherwise call Mindray Customer Service Department or the distributor 7 2 28 Vacuum Error The
7. normal sample BC 2800 Auto Hematology Analyzer V1 1 4 5 Histograms and Pulse Graphs priests 1270885 omet poma MDM a ap arabe Ehi aos page O a a peer ONTE oe Figure 4 24 Interference occurs because gain of PLT channel is too large and data length increases background whe leagh 430038 Cure positon 7168 heute al Press 1 to show rube r Jiro to scrad page T1 00 sand 0 piges ENTER te ext Figure 4 26 Slight interference in WBC channel and data length does not increase obviously normal sample ste lenge 41953 curent ponon 233064 Page 2 t show sier JE to porot 1 page TI to swot 30 pages ENTER i gt enit Figure 4 28 Slight interference in RBC channel and data length does not increase obviously normal sample p lengh moaca current ponina 730618 JL Ju Neve R a show rin GF te emelt pags IAE te etre 8D pages ENTER 10 ait Figure 4 25 Interference occurs because gain of PLT channel is too large data length increases normal sample pt lengh aao curet portion 730518 j J r were F show rin J te emelt pags N te rss pages ENTERI at na LaMar mee Figure 4 27 Slight interference in PLT channel and data length does not increase obviously normal sample whe kagi 4008000 currest posica 3072 4 Press 8 to show nda i to ron page T L te perot 0 pagea ENTER to ext Figure 4 29 Inadequate or no
8. to check the events one by one or press PgUp or PgDn to check the events on the previous or next screen If you want to print out the displayed events press PRINT If you want to acquire help information press HELP For every recorded event the NO column displays the sequences of the recorded events the Time column displays the time when this event occurred the Type column displays the event type the error events are also marked the corresponding error codes that are explained in Chapter 7 1 the Times column displays how many times 1 255 this event occurred and if it occurred more than 255 times the excessive events will be recorded from 1 to another log file the Information column displays extra information regarding the event This analyzer can save maximum 1000 log files and once the maximum number has been reached the newest log will automatically cover the oldest one 6 24 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 6 System Configuration To view the system configuration press MENU to enter the system menu and then press the appropriate arrow keys to move the cursor to Service Config Press ENTER to enter the Config screen as Figure 6 25 shows where you can only view the system configuration without changing it 1 0 0 Ac 000000 Storage capacity 10000 Discriminator Displayed All info Expiration date Clean condition PS 2 keyboard Edit sample infa Printer Transmiss
9. to control the three switches that respectively control the aperture zapping current source and HGB LED Others The Super I O outputs 2 control signals to control the photo couplers of the volumetric metering board and the buzzer of the keypad BC 2800 Auto Hematology Analyzer V1 1 Hardware 1 7 Driving Board The driving board controls and drives the pumps valves and motors 1 7 1 Basic Functions The driving board drives the valves pumps and motors It carries out the following instructions sent by the CPU to open close the pumps or solenoid valves to control the motors of the syringes to control the movement of the sample probe to remain the torques of the motors when the analyzer has entered the screen saver 1 7 2 Basic Blocks The driving board mainly consists of a power block switching block and motor control block See the figure below for the positions of each block of the PCBA 1 7 2 1 Power block The power block includes a 5V 12V and 30V DC supply The 12V and 30V supply comes from the power interfaces where two LEDs are installed to respectively indicate the whether the 12V or 30V supply is connected When the LED is on it indicates the corresponding power has been connected to the driving board The MC7805T converts the received 12V supply into the 5V supply as the following figure shows SV ts MC7805T gt Figure 1 7 How the 5V power is obtained 1 7 2 2 Switching
10. 2004 13 55 Lymph H 41 0 i i Mid 8 0 WB Al 1 f Mode B All Gran 510 0 oo cam 300 fL HGB 124 g L Name RBC 4 08 x 1012 L HCT L 36 0 V 88 3 fL Count Review Maintenance Quality control Setup gt Self test x 10 L Log fL T Calibration Config 10 15 20 25 fL Shutdown Print Reagents for 1 1 7 Select sub menu ENTER Enter sub menu Figure 6 16 Entering status screen Press ENTER to enter the Status screen as Figure 6 17 shows l tem Envir Temp C HGB zerolv HGB blarik v Vacuum Reagents for Figure6 17 Status screen BC 2800 Auto Hematology Analyzer V1 1 6 17 Maintenance Note that you can only view the displayed status items without changing them If any of the displayed item exceeds the given range see Chapter 7 Troubleshooting for solutions 6 4 System Self Test The system self test is a major way to locate system errors Follow the instructions given below to view and check the available self test items Press MENU to enter the system menu and press the appropriate arrow keys t lt to move the cursor to Service Self test as Figure 6 18 shows tem vValue Range Envir Temp C 26 0 15 0 35 0 HGB zerolv 0 01 0 00 0 20 HGB blarik v 4 51 3 40 4 80 Vacuum 187 175 205 Review Maintenance Quality contro gt Status og Calibration
11. 37 1 Dg 50 250 fL Quality contro Status Setup gt Self test Calibration gt Config 5 f Shutdown Print gt 99 Counts Figure 6 1 Entering maintenance screen Press ENTER to enter the Maintenance screen as Figure 6 2 shows tem Item Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe black Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Prime the tubing with diluent Figure 6 2 Maintenance screen If you want to exit this screen press MENU to enter the system menu and access the desired screen from there 6 4 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 2 1 Diluent Prime Biohazard Wear standard laboratory attire including rubber gloves and D4 follow safe laboratory procedures when handling any material in the laboratory You should perform the Diluent prime procedure to prime the diluent tubing with diluent when there are bubbles in the tubing or the diluent in the tubing is contaminated or the old diluent ran out and a new container of diluent is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys t lt to move the cursor to Diluent Prime Press ENTER to prime the tubing with diluent and the priming progress will be displayed at the bottom of the screen as Figure 6 3 shows When t
12. 50uL 10mL Waste exit L l l Probe Wipe Syringe assembly Sample probe _ gt Figure2 5 Washing module washing of sample probe 2 4 3 2 Washing of the front and back baths The part that washes the baths are shown in Figure2 6 The washing consists of two procedures washing the front bath and washing the back bath The front bath is washed by diluent and the back bath by rinse The diluent and the rinse are different and cannot replace each other 2 8 BC 2800 Auto Hematology Analyzer V1 1 Fluidic System Yo Aerial dust 50uL 10mL Dispensing tube Syringe a assembly tube o l l x Metering l l l l EES E Negative pressure chamber Rinse entrance aa lt lt ____ _ Oo j a o Figure2 6 Washing module of fluidic system washing of front and back baths 2 4 4 Hydraulic module The hydraulic module is shown in Figure2 7 This module serves to establish the vacuum and the positive pressure To establish the positive pressure When both valve 1 and the pump are open the pump sucks atmospheric air into it through the upper port and the NC end of valve 1 The sucked pressure is stored in the pressure chamber to establish the positive pressure The pressure value is monitored by the pressure sensor To establish the vacuum When both valve 2 and the pump are open
13. 7 Maintenance to move the cursor to Zap aperture Press ENTER to zap the aperture and the zapping progress will be displayed at the bottom of the screen as Figure 6 6 shows When the zapping is done the screen will return to the initial state Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Zapping aperture Zap aperture Select service Figure 6 6 Zapping aperture 6 2 5 Flush Aperture You can perform the Flush aperture procedure to assist zapping the aperture Follow the steps given below to perform the procedure At the Maintenance screen press the appropriate arrow keys t lt to move the cursor to Flush aperture Press ENTER to flush the aperture and the flushing progress will be displayed at the bottom of the screen as Figure 6 7 shows When the flushing is done the screen will return to the initial state 6 8 BC 2800 Auto Hematology Analyzer V1 1 Maintenance Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe black Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Flushing aperture Figure 6 7 Flushing aperture 6 2 6 Probe Cleanser Cleaning Biohazard Wear standard laborat
14. Fluidic System 2 1 Fluidic system prepares diluent for the whole blood and prediluted modes counts blood cells and measures HGB concentration 1 2 3 dispenses diluent 4 implements the flush startup and cleaning cycles 5 controls vacuum pressure BC 2800 Auto Hematology Analyzer V1 1 2 1 Fluidic System 2 2 Construction of Fluidic System The fluidic system consists of a sample probe assembly syringe assembly pump assembly volumetric metering unit right valve panel and front panel 2 2 1 Sample Probe Assembly a OS No Name 1 Rotation motor D IN a r i 5 gt Lae 2 Probe wipe 3 Probe holder 4 Sample probe 5 Elevator motor 6 Upper photo coupler Sleeve Left and right xe es Le A photo couplers 9 Retaining rin Figure 2 1 Sample probe assembly AAN anng 10 Mount 2 2 2 Syringe assembly 2 2 3 Pump Assembly 2 2 4 Volumetric metering unit 2 2 5 Right valve panel and front panel See Chapter 3 Disassembling Instructions 2 2 BC 2800 Auto Hematology Analyzer V1 1 Fluidic System 2 3 Composition of Fluidic System The fluidic system consists of the following subsystems sensor subsystem bath subsystem lyse dispensing and mixing subsystem diluting subsystem volumetric metering subsystem vacuum subsystem pressure subsystem and auxiliary subsystem The key components of the fluidic system are the solenoid valve syringe aperture s
15. O RINGE TR6A 20 09940 Recorder door TR6D 30 16662 TR60 D drive board 3001 10 07048 Rotation motor 3001 10 07049 Elevator motor 3001 10 07059 Sample probe 3001 10 07252 Diaphragm pump 3001 20 07072 Transformer 3001 20 07245 Three way valve ASCO 3001 20 07246 Two way valve ASCO 3001 30 06880 Sample probe assembly 3001 30 07021 Vacuum chamber assembly 900E 10 04913 Inverter 3001 10 07068 Tubing ID1 16 OD1 8 M90 100035 Tubing IDO 02 ODO 06 M90 10007 1 Tubing ID3 32 OD5 32 8 2 BC 2800 Auto Hematology Analyzer V1 1 Appendix BC 2800 Error Message CODE Error Message Information 0401 Envir Temp Abnormal 0402 Background abnormal HGB error HGB adjust WBC clog WBC bubbles RBC clog RBC bubbles Communication error Scanner error Scanner communication error Printer out of paper Printer connection error Recorder communication error Recorder out of paper Recorder too hot Press bar up Lyse out Diluent expired Rinse expired Lyse expired Filer error Real time clock error Syringe motor error Rotation motor error Elevator motor error A D error Vacuum error Press error 400B Diluent out 400D Waste full 8002 Dynamic memory error 8001 File error P N 2800 20 28832 1 1
16. See the block diagram above for details BC 2800 Auto Hematology Analyzer V1 1 Hardware 4 Drive signal detection circuit of the position transducers The control system judges the motor positions by the signals sent by the position transducers photocouplers The photocouplers are driven by the MCU through a 74LS07 The photocoupler sends the position signals to the MCU through a 74LS14 inverter See the figure below for the block diagram of the position detecting circuit The photocouplers are installed on the sample probe assembly and the syringe assembly and feed the control and feedback signals to the driving board through cables 1 7 2 4 Testable Signals To test the signals connect the grounding terminals of the oscillator and the multi meter to the DGND or the PGND The testable signals are control signals of valves and pumps sequence signals of the motors valves position signals sent by the position transducers serial communication signals reset signals and voltage signals of the power supplies BC 2800 Auto Hematology Analyzer V1 1 1 13 Hardware 1 8 Display Unit 1 8 1 Function of the Adapter The LCD adapter connects the LCD to the CPU board 9 interface board _ gt connecting cables CPU board Figure 1 9 Connection Schematic 1 8 2 Introduction of the adapter The adapter incorporates two FPC FFC connectors J2 and J3 The J3 is for the BC 2800 display while the J2 is reserved f
17. X4 and X2 are external crystal oscillators whose frequencies are 45MHz 45MHz and 24MHz respectively The clock output of the CPU BCLKO is main clock signal of the CPU board 1 4 BC 2800 Auto Hematology Analyzer V1 1 Hardware 1 4 CPU and Peripheral Devices DRAM CRTL Clock Driver SRAM BUS CONTROL DRC gt BUS Interface Figure 1 4 CPU and peripheral devices 1 4 1 CPU gt The CPU is MOTOROLA MCF5307 external frequency 45MHz operation frequency 90MHz processing speed as high as 75MIPS gt The MCF5307 features a 32 bit data bus and a 32 bit address bus The board uses a 24 bit addressing mode reserving the most significant 8 bits as the general purpose I Os for the FPGA gt The MCF5307 can be tuned through the BDM port J18 of the CPU board gt The CPU board utilizes the built in C and UART controllers of the MCF5307 to use the EEPROM and RTC as expanded serials ports gt The CPU boards utilizes the built in DRMA controller of the MCF5307 to use the 2x8M SDRAM as the expanded memory 1 4 2 WDT The Watch Dog Timer WDT is Tl TPS3828 It monitors the running of the software The CPU must send a feedback to the WDT every 1 6s otherwise the WDT will force the CPU to restart BC 2800 Auto Hematology Analyzer V1 1 1 5 Hardware MCF5307 PPY yJ nRSTI Figure 1 5 WDT 1 4 3 FLASH The FLASH is TE28F160 2M bytes The boot program is sto
18. analyze the reason leading to the fault by viewing these original data Common pulse graphs Enter password 3210 after a count you can view the WBC pulse graph of this count by pressing 1 and RBC pulse graph by pressing 2 and PLT pulse graph by pressing 3 Press ENTER to exit When the instrument is working normally the length of pulse data is related to the concentration of the blood sample The length of the pulse data should be within a limit range For general samples the range should be WBC lt 1M RBC lt 600K PLT lt 1M Data length of abnormal sample will not lie in this range Length of normal level controls data should be WBC 400 700K RBC 250 450K PLT 300 600K Normal pulse graphs Ny TF ii HAT ilh H sah N MND N Ni i N li HL Mh A Nays M Areeni f Kal l tel AN IAR MA 4 VINN UAL f Fress a to sus i oe cnr Figure 4 12 WBC pulses Figure 4 13 PLT pulses rhe lengh 119549 asma penbon Te vecin ne Curemtpomma 3022 Te AN WH A i pag TTA vert a Figure 4 14 RBC pulses Figure 4 15 Normal WBC background pulse BC 2800 Auto Hematology Analyzer V1 1 4 3 Histograms and Pulse Graphs Pee Press B mow ron 99 3 to show P Ju paoti pp 4 w ema o a T we lew i o 0 scrol page TI to sorot JOpazes ENTEN torm EES Figure 4 16 Figure 4 17 Pulse of normal RBC background check Pulse of normal
19. block The switching block mainly consists the photocoupling circuit and driving circuit of the pumps and valves as the figure below shows Photo coupling Isolation Drive circuit circuit Control Drive signal signal Figure 1 8 Switching control circuit 1 Photocoupling circuit The photocoupling circuit mainly consists of the photocoupler and resistors It provides 16 TTL outputs to the valves and pumps The photocoupler BC 2800 Auto Hematology Analyzer V 1 1 1 11 Hardware 2 TLP521 2 isolates the digital ground from the driving ground Driving circuit of valves and pumps The driving voltage of the valves and pumps are 12V TTL The circuit mainly consists of ULN2068 In the BC 2800 the circuit can maximum drives 14 valves and 2 pumps The fluidic system decides which pumps or valves are to be actually used 1 7 2 3 Motor control block The motor control block includes serial communication circuit control drive circuit of the sample probe mechanism control drive circuit of the syringe motor and drive signal detecting circuit of the position sensors Serial communication circuit Since the CPU board requires a 3 3V power supply while the driving board requires a 5V power supply a photocoupler H11L1 is needed for the purposes of conversion and isolation Control Drive circuit of sample probe mechanism The control Drive circuit of sample probe mechanism includes the control drive circuit of the
20. gt Config Shutdown Print Figure 6 18 Entering self test screen Press ENTER to enter the Self test screen as Figure 6 19 shows If you want acquire help information press HELP 6 18 BC 2800 Auto Hematology Analyzer V1 1 Maintenance Tubing Result O Machine i O Valve Circuit 7 Figure 6 19 Self test screen The available self test items are divided into four groups the Tubing Machine Valve and Circuit as shown on the left of the screen You can switch among the groups by pressing F1 and once a group is selected the included test items together with their test results if available will be displayed on the right of the screen You may press PRINT to print out the displayed results Follow the instructions given below to conduct every test and if you want to acquire help information press HELP 6 4 1 Testing Tubing At the self test screen press F1 to select the Tubing group as Figure 6 19 shows The following test items will be displayed on the right of the screen Count Time It measures the duration of a WBC and RBC count namely how many seconds it takes for the aspirated fluid flows from the first sensor to the second Aperture v It measures the voltage v over the aperture Vacuum It checks whether the vacuum system functions normally Pressure It checks whether the system flushes the aperture at a normal press
21. may press ENTER to stop the process any time Note that a shortened soaking process may not be as effective as a complete one When the soaking is done press ENTER to flush the bath and tubing after which the screen will return to the initial state If you analyzer has been running continuously for 24 hours a dialog box will pop up to remind you to perform the Probe cleanser cleaning procedure If you want to do so move the cursor to Yes and press ENTER Otherwise move the cursor to No and press ENTER 6 2 8 Lyse Test Biohazard Wear standard laboratory attire including rubber gloves and follow safe laboratory procedures when handling any material in the laboratory In case of any abnormal WBC counts or histograms you can perform the Lyse test procedure to check whether the lyse can be dispensed properly Follow the steps given below to do so Unscrew and remove the retaining screws with hands or screwdrivers pointed by the arrows shown in Figure 6 9 on the right plate SMITH NMHYS WA Cx ZA Figure6 9 Removing the two screws Follow the arrow shown in Figure 6 10 to push and remove the right plate BC 2800 Auto Hematology Analyzer V1 1 6 11 Maintenance Figure 6 10 Removing right plate Remove the screws fixing the shielding box of the bath as Figure 6 11 shows Figure 6 11 Shielding box Remove the shielding box to expose the bath as Figure 6 12 shows 6 1
22. so move the cursor to Yes and press ENTER otherwise move the cursor to No and press ENTER Whole Blood AlIl Current sample WBC L 0 3 x 10L Lymph wee 1 AL Soha ras Midi reese x 10 W 21 Gran see DAL Time 01 28 2004 00 17 Lymph t z Mid fe 0 100 200 300 fL Gran OR Mode WB All Name Gender Ape 150 250 fL Next sample ROW CVY H 23 59 ROW SD 44 9 fL 5 a n PLT H 443 x 10L Wwe MPY 9 4 fL N WB All inan B PDW H 17 4 PCT H 0 416 0 5 10 15 20 25 fL Figure 6 8 Recommending probe cleanser cleaning 6 2 7 E Z Cleanser Cleaning Biohazard om A Wear standard laboratory attire including rubber gloves and follow safe laboratory procedures when handling any material in the laboratory You can use the E Z cleanser an enzyme based isotonic cleaning solution and wetting agent to clean the tubing and bath by performing the E Z cleanser cleaning procedure Follow the steps given below to perform the procedure At the Maintenance screen press the appropriate arrow keys f J lt to move the cursor to E Z cleanser cleaning Present the cleanser to the probe and press ENTER to aspirate the cleanser Remove the cleanser after the probe has risen up This analyzer will automatically soak the bath and tubing with the aspirated cleanser 6 10 BC 2800 Auto Hematology Analyzer V1 1 Maintenance The soaking process will last about 10 minutes and you
23. system does not reach the expected vacuum within the given time Solution Access Service Self test Tubing and test the vacuum The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor 7 2 29 Pressure Error The vacuum chamber does not reach the expected pressure within the given time Solution Access Service Self test Tubing and test the pressure The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor 7 8 BC 2800 Auto Hematology Analyzer V1 1 Troubleshooting 7 2 30 Diluent Out Possible causes insufficient diluent or wrong diluent volume setting Solution Check if there is sufficient diluent left If so access Setup Settings Reagents and adjust the remaining diluent volume if not change a new container of diluent 7 2 31 Rinse Out Possible causes insufficient rinse or wrong rinse volume setting Solution Check if there is sufficient rinse left If so access Setup gt Settings Reagents and adjust the remaining rinse volume if not change a new container of rinse 7 2 32 Waste Full The waste container is full Empty the container or change a new container to receive the waste and re set the waste container volume 7 2 33 File Error Something is wrong with file saving Turn off the analyzer and call Mindray Customer Service Department o
24. up from the bottom of the bath and the mixing is done thus The on off interval of the valve is critical to effect of the bubble mixing Either too many or too few bubbles will affect the mixing During the mixing process the airway should be well drained or the trapped liquid will affect the quantity of the bubbles as well as the dilution 2 10 BC 2800 Auto Hematology Analyzer V1 1 Fluidic System Positive pressure Bath assembly Negative pressure chamber Figure2 8 Mixing module 2 4 6 Waste discharging module The waste discharging includes discharging waste of the probe wipe discharging the waste of the bath discharging the waste of pressure chamber As Figure2 9 shows once the negative pressure is established inside the pressure chamber valve 11 will open to discharge the waste of the bath to the outside through the pressure chamber and the pump Likewise the waste of the probe wipe is discharged to the outside when valves 9 and 10 are open Bath gt assembly Negative pressure chamber Figure2 9 Waste discharging module BC 2800 Auto Hematology Analyzer V1 1 2 11 Fluidic System 2 5 Counting Procedure 2 5 1 Dilution Usually in blood samples the cells are too close to each other to be identified or measured For this reason the diluent is used to separate the cells so that they are drawn through the aperture one at a time as well as to create a conductive environment for blood a
25. 0 fL PLT 2 30 fL BC 2800 Auto Hematology Analyzer V1 1 2 13 Disassembling the Analyzer Chapter3 Disassembling the Analyzer 3 1 Main Unit Structure 3 1 1 Main user interfaces Front view 1 LCD 2 Keypad 3 Recorder 4 Power Indicator 5 START key 6 Sample Probe Figure 3 1 Front view N m 5 p lt 2 on an an a Rear view 1 Power switch r m 2 Equipotentiality 3 Waste Outlet Red 4 Lyse Inlet Orange a 5 Rinse Inlet Blue 6 Diluent Inlet i i Green ob eri A d L Figure3 2 Rear view BC 2800 Auto Hematology Analyzer V1 1 3 1 Disassembling the Analyzer Left view PP 1 RS232 Port1 for the scanner 2 Parallel Port 3 RS232 Port 2 for the computer 4 Power Interface of Floppy Disk Drive 5 Keyboard Interface Figure3 3 Left view 3 2 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer 3 2 Disassembling the Main Unit 3 2 1 Removing the left right and top covers As the figure below shows remove the retaining screws two for each cover with a Philips screwdriver and pull the covers away 4 ep Pra j P i p a l l A B gt A Gi 0 eae J 2 q co lt n 7 W J iN Z Figure3 4 Removing the left right and top covers 3 2 2 Removing the back plate and the f
26. 2 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 2 9 Figure 6 12 Bath At the Maintenance screen press the appropriate arrow keys t lt to move the cursor to Lyse test Press ENTER and this analyzer will automatically drain the bath and then dispense 2ml lyse to the bath Check the scale to see whether the lyse has reached the expected line the second from bottom In case the dispensed lyse has failed to reach this line for several consecutive times check whether the lyse has run out or the lyse tubing is not properly connected to this analyzer If the lyse is still enough and the tubing is well connected to the analyzer contact the Mindray Customer Service Department for repair Press ENTER to flush the bath and finish the test The screen will return to the initial state Clean Bath When you keep finding abnormal results from the background check follow the steps given below to perform the Clean Bath procedure At the Maintenance screen press the appropriate arrow keys t lt to move the cursor to Clean bath Press ENTER to start the procedure When the cleaning is done the screen returns to the initial state 6 2 10 Drain Bath The Drain Bath procedure checks whether the analyzer can drain the bath within the given time You can perform this procedure when you find three or more of the HGB WBC RBC and PLT results are abnormal Follow the first 4 steps of performi
27. 2051 whose resetting time is 470ms It uses a 11 0592MHz crystal oscillator 1 9 2 3 Keypad scanning The keypad matrix is 5X4 one incorporating 9 I O wires and 20 keys Note that the keys at line 5 and columns 3 and 4 are not used 1 9 2 4 Backlight control The keypad adapter shuts off the backlight and blinks the power indicator when instructed by the main board to do so usually after the analyzer entered the screen saver The backlight board uses an independent 12V power supply and receives the control signals through photocouplers The transistor is used to help control the LED so that the power indicator can be turned on even when something is wrong with the MCU 1 16 BC 2800 Auto Hematology Analyzer V1 1 Hardware YOO Figure 1 11 Control of LCD brightness The LCD brightness is controlled by pot RV1 Adjusting the RV1 can force the VBL to change within 0 5 3V The voltage change is fed into the inverter and causes the change of the drive current and hence the change of the brightness Note that the smaller the voltage and the brighter the LCD 1 9 2 5 Buzzer control The buzzer is controlled by a DC signal 5V DC current lt 40mA The 5V supply of the buzzer is isolated from the VDD and the control signal is received through a photocoupler TLP521 2 that is controlled by a current around 10mA AAT HALTI Figure 1 12 Buzzer control BC 2800 Auto Hematology Analyzer V1 1 1 17 Fluidic System Chapter2
28. 8 indicate the system status as defined by the software BC 2800 Auto Hematology Analyzer V1 1 1 7 Hardware 1 5 Analog Inputs and Outputs 1 5 1 Signals of Blood Cell Counts The CPU board has three A D converters U10 AD7928 U11 AD7908 and U14 AD7908 Both the AD7928 and AD7908 feature 8 channel and 1MSPS only the former is 12 bit converter and the latter 8 bit The U10 is actually installed and powered by a 2 5V supply while the U11 and U14 are reserved The sampling speed is set to 500KSPS 1 5 2 Signals of System Monitoring The Super I O monitors such system status as the 48V 12V and 12V supplies of the analog board the 3 3V and 12V supplies of the CPU board itself and the temperature of the whole analyzer 1 5 3 Signals of LCD Contrast The Super I O generates PWM signals that are then integrated to output a 0 2 5V analog signal to control the LCD contrast The user can adjust the contrast through the software interface 1 8 BC 2800 Auto Hematology Analyzer V1 1 Hardware 1 6 Digital Inputs and Outputs 1 6 1 Serial Port The analyzer has 6 serial ports which are illustrated in Figure 1 6 COMO CELUI ais COM COM FPGA eer 2 COMS ae COMSB Super I O COMA Figure 1 6 Serial ports The CPU incorporates 2 UART controllers 3 3LVTTL one to control the motor of the driving board and the other communicates with the recorder powered by 5VTTL The FPGA implemen
29. 845 Locking nut of 7 5ml syringe 2800 20 28846 Body of 50ul syringe 2800 20 28847 Nut of 50ul syringe 2800 20 28848 Bolt of 50ul syringe 2800 20 28849 Connection wire of inverter 2800 21 28728 Shielding box cover assembly 2800 21 28788 Shielding box assembly 2800 30 28650 CPU board 2800 30 28652 Analog board 2800 30 28654 2800 30 28664 Driving board Keypad BC 2800 Auto Hematology Analyzer V1 1 8 1 List of Spare parts 2800 30 28668 Volumemetric metering board 2800 30 28670 Power board 2800 30 28715 2800 30 28716 Screen assembly Sampling assembly 2800 30 28746 LCD adaptor 2800 30 28767 Bath assembly 2800 30 28778 Syringe assembly 2800 30 28779 7 5ml syringe assembly 2800 30 28780 50ul syringe assembly 2800 30 28789 Bath mount assembly 2800 30 28815 HGB mount assembly 2800 30 28817 CAP component for LYSE 2800 30 28818 CAP component for DILUENT 2800 30 28819 CAP component for RINSE 2800 30 28839 Waste tubing assembly 3001 10 07252 Vacuum Pump 3001 20 07247 Localizer 3001 21 07182 Cap for LYSE 3001 30 06957 Probe wipe 59BR 10 08830 Thermal head Mg90 000130 O RING 6x1 M90 100054 O RING 2 00X1 80 M90 100069 P N X420 1 10 32 Special Tapered Thread To 3 32 Mg90 100101 Y shaped
30. ARTICULAR PURPOSE Exemptions Mindray s obligation or liability under this warranty does not include any transportation or other charges or liability for direct indirect or consequential damages or delay resulting from the improper use or application of the product or the substitution upon it of parts or accessories not approved by Mindray or repaired by anyone other than a Mindray authorized representative This warranty shall not extend to any instrument which has been subjected to misuse negligence or accident any instrument from which Mindray s original serial number tag or product identification markings have been altered or removed or any product of any other manufacturer Safety Reliability and Performance Mindray is not responsible for the effects on safety reliability and performance of the BC 2800 Hematology Analyzer if M assembly operations extensions re adjusts modifications or repairs are carried out by persons other than those authorized by Mindray E Personnel unauthorized by Mindray repairs or modifies the instrument BC 2800 Auto Hematology Analyzer V1 1 III Copyright Return Policy Return Procedure In the event that it becomes necessary to return a unit to Mindray the following procedure should be followed 1 Obtain return authorization Contact the Mindray Service Department and obtain a Customer Service Authorization Mindray number The Mindray number must appear on the outside of the sh
31. BC 2800 Auto Hematology Analyzer Service Manual Copyright Copyright Statement SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD hereinafter called Mindray owns all rights to this unpublished work and intends to maintain this work as confidential Mindray may also seek to maintain this work as an unpublished copyright This publication is to be used solely for the purposes of reference operation maintenance or repair of Mindray equipment No part of this can be disseminated for other purposes In the event of inadvertent or deliberate publication Mindray intends to enforce its rights to this work under copyright laws as a published work Those having access to this work may not copy use or disclose the information in this work unless expressly authorized by Mindray to do so All information contained in this publication is believed to be correct Mindray shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material This publication may refer to information and protected by copyrights or patents and does not convey any license under the patent rights of Mindray nor the rights of others Mindray does not assume any liability arising out of any infringements of patents or other rights of third parties Content of this manual is subject to changes without prior notice PROPERTY OF SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS
32. CO LTD ALL RIGHTS RESERVED Responsibility on the manufacturer party Mindray is responsible for safety reliability and performance of this equipment only in the condition that e all installation expansion change modification and repair of this equipment are conducted by Mindray qualified personnel e applied electrical appliance is in compliance with relevant National Standards e the instrument is operated under strict observance of this manual BC 2800 Auto Hematology Analyzer V1 1 Copyright A Note AY This equipment is not intended for family usage This equipment must be operated by skilled trained clinical personnel A Warning A It is important for the hospital or organization that employs this equipment to carry out a reasonable maintenance schedule Neglect of this may result in machine breakdown or injury of human health Il BC 2800 Auto Hematology Analyzer V1 1 Copyright Warranty Workmanship amp Materials Mindray guarantees new equipment other than accessories to be free from defects in workmanship and materials for a period of one year from date of shipment under normal use and service Mindray s obligation under this warranty is limited to repairing at Mindray s option any part which upon Mindray s examination proves defective THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY P
33. Drain tubing Flush aperture Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Cleaning wipe block Figure 6 14 Cleaning wipe block When the soaking is done wipe the bottom of the wipe block with a probe cleanser dipped cloth that does not leave debris Press ENTER to flush the block and the inner surface of the probe After the flushing is done the screen returns to the initial state When the accumulated analyzed samples reach 4 000 a dialog box will pop up to remind to clean the wipe block You may move the cursor to Yes and press ENTER to start the cleaning or to No and press ENTER to ignore the message BC 2800 Auto Hematology Analyzer V1 1 6 15 Maintenance 6 2 13 Preparing to Ship If this analyzer is not to be used for over two weeks or is to be shipped perform the Prepare to ship procedure to flush and drain it Follow the steps given below to do so Remove the diluent rinse and lyse tubing from their containers At the Maintenance screen press the appropriate arrow keys t J lt to move the cursor to Prepare to ship Press ENTER and a dialog box will pop up to ask you to confirm this operation as Figure 6 15 shows Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Flush aperture Probe cleanser c 7 Prepare to Sip E Z cleanser cle Remove the tubin e and lyse containers
34. PLT background check 4 4 BC 2800 Auto Hematology Analyzer V1 1 Histograms and Pulse Graphs Abnormal pulse graphs Carer postion 15306 irene tinea timate Pers F t show nier Pp to enog page TILI eroe 20 pages ENTIM iora Figure 4 18 Severe interference in WBC channel and data length increases obviously background ureri potice 13324 Press 7 te stow nas Pp 80 scre8 page T 1 eros 28 pages ENTER to ext Figure 4 20 Severe interference in RBC channel and data length increases obviously background PEER E TA TTET Paese F to show rder P io smd p Figure 4 22 Severe interference in P LT channel and data length increases obviously background whe length 7205314 Cures poston 15640 T LW J o J Press 7 le stow rer lonni paga MEL poras papa OTER po me 4 Figure 4 19 Severe interference in WBC channel and data length increases obviously normal sample st ength 1123968 current potna T680 Vuh i da j Treva fi abow ruler CE be enoa paga 1I to rE 0 pages ENTER 10 w Figure 4 21 Severe interference in RBC channel and data length increases obviously normal sample pt leh 261000 eurez pores 128000 4 j Villesi ENA bu Pme F t show rder P Tor to panogi pagr TI to cre 0 papra ENTEN IO exe Figure 4 23 Severe interference in PLT channel and data length increases obviously
35. ample probe pump bath metering tube probe module negative positive pressure chamber and hose The solenoid valve is ASCO458 Totally 11 valves are used 7 three way valves and 4 two way valves The two syringes 10mL and 50uL are made by Mindray The aperture is O80 The sample probe has two layers and its surface is polished making the probe easy to clean BC 2800 Auto Hematology Analyzer V1 1 2 3 Fluidic System 2 4 Functional modules The fluidic system can be divided into the following functional modules aspiration dispensing module counting module washing module hydraulic module mixing module and waste discharging module See the figure below for the interaction of these modules Reagent Washing module module Counting Quantification Mixing module Sample Hydraulic module discharging Figure2 2 Interaction of the functional modules 2 4 1 Aspiration dispensing module The aspiration dispensing module includes a motor that drives the 50 uL and 10 mL syringes the former for aspirating dispensing the whole blood sample and the latter for aspirating dispensing the prediluted sample diluent and lyse See the figure below for details BC 2800 Auto Hematology Analyzer V1 1 Fluidic System Buffer tubing j C NC N c NC Cc NO c CRO NO NO Buffer tubi
36. ample probe if you have replaced the wipe block or observed motor error or wrong test result Also as required by regular maintenance you should use the localizer to adjust the position of the sample probe monthly Figure3 17 Localizer 3 2 15 Adjusting Sample Probe Position Biohazard Wear standard laboratory attire including rubber gloves and follow safe laboratory procedures when handling any material in the laboratory 3 10 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Follow the steps given below to adjust the sample probe position 1 Refer to the steps mentioned in section 3 2 1 to remove the right cover of the analyzer 2 Access the Setup Password screen and enter the administrator password to obtain the administrator authority Access the Service Self test screen Press F1 to select the Machine group and press f or to move the cursor to Elevator motor as the figure below shows Tubing I Item Result Machine Syringe motor O Valve n O Circuit Rotation motor Elevator motor Print Select test ENTER Execute Figure3 18 Accessing elevator motor Press ENTER and an elevator motor screen will pop up as the figure below shows BC 2800 Au
37. boa 81920 Fe Bjo owner el to scro8 8 page TNL be serol 50 pates ENTER 9 ext Figure 4 38 Interference in WBC channel from tubing Feature data length increases the base line of signal is not stable p lengt 2043000 curent pcsion 151000 Veen J M Vna plat anny Iarr aati OO Pass 3 to show eater J tose o I page TE to srol 30 pages INTER io ent Figure 4 37 Interference in PLT channel from tubing Feature data length increases the base line of signal is not stable the length 2043000 carent porhor 1152000 Prese to tow nda m 80 srol pare TLL 80 scroll pages ENTER to ext Figure 4 39 Interference in RBC channel from tubing Feature data length increases the base line of signal is not stable 4 8 BC 2800 Auto Hematology Analyzer V1 1 Adjustment Chapter5 Adjustment This chapter deals with gain adjustment and hardware adjustment 5 1 Gain Adjustment 5 1 1 Adjust the channel gains if you replace the below parts the bath s the aperture s the analog board the Disk on Module Method 1 Using normal patient fresh blood or calibrator to check the gains 2 Control the gains through the digital potential meter Replacement of the analog board will affect WBC whole blood WBC prediluted RBC HGB PLT results Replacement of the bath s will affect WBC whole blood WBC prediluted RBC HGB PLT Repla
38. cement of the aperture s or gasket s will affect WBC whole blood WBC prediluted RBC PLT Enter the Password screen and enter the password 3210 Count Review Quality contro Service Calibration Shutdown PW level service Press MENU to enter the system menu move the cursor to Setup Gain and press ENTER to enter the Gain screen BC 2800 Auto Hematology Analyzer V1 1 5 1 Adjustment Setup Settings Caunt Review Quality contro Service Calibration Shutdown Reagent Print amp comm Date amp time 2 Wain gt Count Ref range Other CoOo0e000 Reagent Digipot Print amp comm Rate Date amp time Gain Count Ref range Other 100 0 100 0 WBC WB WBC PB REC HGB 109 7 P a gt A gt HGB blank V MENU 7 Move cursor T Displayed values current gains Range adjustable range Rate changing factor compared to the old value Reagents for Select setting item F1 Select setting group gt 99 Counts WBC whole blood gain of the WBC channel in the whole blood mode WBC prediluted gain of the WBC channel in the prediluted mode RBC gain of the RBC channel HGB gain of the HGB gain PLT gain of the PLT channel BC 2800 Auto Hematology Analyzer V1 1 Maintenance Cha
39. creen 6 26 BC 2800 Auto Hematology Analyzer V1 1 Maintenance You can perform the following operations at the Print screen Press f or to select the desired task Press DEL to delete the selected task Press HELP to display the help information Press MENU to return to the system menu BC 2800 Auto Hematology Analyzer V1 1 6 27 Maintenance 6 8 Adjusting Sample Probe and Replacing Probe Wipe See Chapter 3 3 6 28 BC 2800 Auto Hematology Analyzer V1 1 Troubleshooting Chapter7 Troubleshooting The chapter deals with the codes possible causes and solutions of the errors If the error remains after you have tried the recommended method call Mindray Customer Service Department or the distributor This chapter consists of two parts the first part dealing with the errors and assigned error codes and the second possible causes and recommended solutions BC 2800 Auto Hematology Analyzer V1 1 7 1 Troubleshooting 7 1 Error Codes The errors recorded in the log are presented in the error codes See Table 7 1 for the correspondence between the errors and error codes Table 7 1 Errors and codes Environmental Background Temperature 0403 HGB error Abnormal 0404 HGB adjust WBC clog 0406 WBC bubbles 0407 RBC clog RBC bubbles Communication Scanner 0801 Scanner error 0803 ar error communication error Printer Printer out of l Recorder 1001 connection 1003 PEON pa
40. e accordingly After the adjustment test the actual count time again and make sure the difference is within 2 seconds If the error still remains call Mindray Customer Service Department or the distributor RBC Bubbles This error message occurs when the actual RBC count time is less than the preset RBC count time by 2 seconds Possible causes 1 Insufficient diluent or rinse 2 Loose tubing connection 3 Inappropriate RBC count time setting Solution Check if the diluent or rinse is sufficient If not change a new container of diluent of rinse Check the tubing connections If necessary reconnect the tubing If the error still remains access Setup Password to gain the administrator authority and then access Setup Settings Count and adjust the RBC count time If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor BC 2800 Auto Hematology Analyzer V1 1 7 5 Troubleshooting 7 2 9 Communication Error The received communication settings are different with the BC 2800 Solution Access Setup Settings Print amp comm and change the communication settings accordingly 7 2 10 Scanner Error Too long or invalid bar code Solution Check and make sure the bar code is correct If the error still remains call Mindray Customer Service Department or the distributor 7 2 11 Scanner Communication Error Something is wrong w
41. e unclogging is successful and you can return to the Count screen to continue the analysis Otherwise Access Service Maintenance to soak the bath and tubing with probe cleanser When the soaking is done access Setup Settings Count to note down the preset WBC count time Access Service Self test Tubing and test the actual WBC count time If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis If the difference is still greater than 2 seconds and stabilized around a certain value access Setup Settings gt Count to change the WBC count time accordingly After the adjustment test the actual count time again and make sure the difference is within 2 seconds If the error still remains call Mindray Customer Service Department or the distributor 7 2 6 WBC Bubbles This error message occurs when the actual WBC count time is less than the preset WBC count time by 2 seconds Possible causes Insufficient diluent or rinse Loose tubing connection Inappropriate WBC count time setting Solution Check if the diluent or rinse is sufficient If not change a new container of diluent of rinse Check the tubing connections If necessary reconnect the tubing If the error still remains access Setup Password to gain the administrator authority and then access Setup Settings Count and adjust the WBC co
42. eeeeeeeeeeeeeeeeeeeeees 1 16 C hapter2 Fluidie Sy Stem iccastecnesecc duase vacetdanbadetdevtacesdautccetdevtacesdautacetdevtecunds 2 1 2 1 Eluidie systemi ion glad te d oe oi bed oi edo hed a ed id bed od bans 2 1 2 2 Construction of Fluidic System 2 2 c c ceeeeeeeeeeeeneeceeeeseeeeeeeceee 2 2 2 3 Composition of Fluidic System eee eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee 2 3 2 4 Functional modules 3 c xp gscceaccesargeassect chen saevasevsneveneetustausseuavenerdasteneesuenaad 2 4 2 5 Counting Procedure ein cesta eee eee ee 2 12 Chapter3 Disassembling the Analyzer eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneeeeees 3 1 3 1 Main Unit Structure oad oder cnredereat rath Seid ted ideadeacesecttededeutsaceiachentedeutdacatatts 3 1 3 2 Disassembling the Main Uniit c cece cee ete cette tceeeceeeeeeeceeenes 3 3 Chapter4 Histograms and Pulse Graphs ccccccccceeeeeeeeeeeeeeeeeeeeeeeeeees 4 1 4 1 Histograms crit tees Gaeta tive lite nie hee i e 4 1 4 2 Pulse Graphs ienei aa a aa aaa adnate anaes 4 3 Chapters Adj StMENt onene ek reee EEEE EEEE EEEE EEEE E Eeh 5 1 5 1 Gain Adjustment nenene 5 1 Chapter6 Maintenance ss cas ccacd2pnes at snsgiasdonnecntosnieaudtosei esol nelaeeeieeletens 6 1 6 1 Regular Maintenance ccs c cote 2c odeoccedeoricedessesstuaeducateadu cates steaduende 6 2 6 2 System MAINIOMANCE noieeoe eoero eardere ieaie ardea 6 4 Bs System StatUS re ie aaa a e ad i dd Ded 6 17 0 4
43. elevator motor and that of the rotation motor The control system of the sample probe motor consists of a AT89S51 MCU and ADM705 WDT The AT89S51 also detects the signals coming from the position sensor when controlling the motors Control drive circuit of the elevator motor The circuit includes the control part a MCU system and the drive part as shown in the figure below The MCU system provides the sequence signals for the elevator and rotation motors and controls the position sensors as the figure below shows The MCU reset signal RST_XY is active high The drive part mainly consists of a control device L6506 drive device L298N and follow current device UC3610 The drive voltage is 30V The sequence signal and the enable signal of the drive device come from the MCU Control drive circuit of the rotation motor The circuit mainly consists of a control part MCU system and a drive part Refer to the previous introduction for the MCU system The drive part is the ULN2068B and the drive voltage is 12V The sequence signal comes from the MCU as shown in the figure below Control drive circuit of the syringe motor The circuit mainly consists of a control part MCU system and a drive part The MCU is the P87LPC762 with built in WDT The MCU system executes the aspirating and dispensing operation of the syringe and detects the signals sent by the position transducers The drive part is similar to that of the elevator motor
44. ells passing the aperture The detected pulses are then sent to the analog board to be amplified rectified recognized adjusted and counted When the sample is mixed in the bath valve 2 is open and the negative pressure chamber introduces the atmospheric air to pass through the filter and valve 2 and then to flush the metering tube Valve 1 is open when the vacuum has been established and the sample blood cells in the bath is drawn through the aperture by the negative pressure to generate the counting pulses The sample keeps moving to push the rinse between the back bath and metering tube to move through the tube When the rinse passes the upper optical sensor mounted on the metering tube a start signal is generated and sent to the analog board which starts the counting right away and when the rinse passes the lower optical sensor a stop signal is generated and sent to the analog board which stops the counting right away Volumetric metering the volumetric metering ensures a relatively objective and stable analysis cycle Monitoring of the counting time the volumetric metering enables the monitoring of the counting time By monitoring the counting time the system can easily know whether the aperture is clean or blocked and feed this information to the service personnel in terms of the aperture voltage so that they can service the 2 6 BC 2800 Auto Hematology Analyzer V1 1 Fluidic System analyzer in time 2 4 3 Washing mod
45. gurations are stored in the EEPROM The VRAM is the memory for video display 1 1 2 Basic Functions of the CPU Board To receive such analog signals as the WBC RBC PLT counts HGB measurement aperture voltage vacuum pressure signals etc To monitor such system status as the 48V 12V and 12V supplies of the analog board the 3 3V and 12V supplies of the CPU board itself and the temperature of the whole analyzer To receive the keypad signal and control the keypad buzzer and LCD backlight BC 2800 Auto Hematology Analyzer V1 1 1 1 Hardware To generate control signals to control the valves aperture zapping HGB LED current source and digital pot To drive and turn on the LCD and adjust the contrast To drive the keyboard printer and floppy drive 1 2 BC 2800 Auto Hematology Analyzer V1 1 Hardware 1 2 Power Supply The CPU board is powered by two independent external power supplies a 5V supply and a 12V supply Two 5A fuses are respectively installed on the two power entries The 5V supply is converted a 3 3V supply to power the digital components and the 3 3V supply is also further converted into a 1 5V supply to power the FPGA The 12 8V supply serves the CPU board only Power socket Figure 1 2 Power distribution of the CPU board BC 2800 Auto Hematology Analyzer V1 1 1 3 Hardware 1 3 RTC VARM SDRAM Synchronous clock Figure 1 3 Arrangement of the CPU Clock The X1
46. he priming is done the screen will return to the initial state p ent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming diluent Diluent prime Select service Figure 6 3 Diluent priming screen BC 2800 Auto Hematology Analyzer V1 1 6 5 Maintenance 6 2 2 Rinse Prime Biohazard Ca Wear standard laboratory attire including rubber gloves and D4 follow safe laboratory procedures when handling any material in the laboratory You should perform the Rinse prime procedure to prime the rinse tubing with rinse when there are bubbles in the tubing or the rinse in the tubing is contaminated or the old rise ran out and a new container of rinse is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys t JE J lt to move the cursor to Rinse prime Press ENTER to prime the tubing with rinse and the priming progress will be displayed at the bottom of the screen as Figure 6 4 shows When the priming is done the screen will return to the initial state tem Diluent prime Lyse test Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming rinse
47. hemolysis in WBC channel and data length increases 4 6 BC 2800 Auto Hematology Analyzer V1 1 Histograms and Pulse Graphs pt length 2048000 arent porhon 4608 Ten Figure 4 30 Too high sample concentration in the RBC channel Figure 4 31 Too high concentration in the it normally does not appear PLT channel it normally does not appear wde length 2088000 Curent potion 564228 whe length 362076 cwrte potan 370668 wherval Sou hgh 100 AAAI ANA A rae Pues i D show ater DANI te select rder 2 to Bade ndee fji te sero 1 page T1 to srol 30 pages ENTER to est hij tomore opace TL to move 10 eposen ENTER to est Figure 4 32 Interference in WBC channel Figure 4 33 Measuring interference from caused by inverter Feature sine wave inverter with cycle of 20 26us whe length 2379108 carent ponton 73767 rhc length 183886 Cusrest pontica 179712 AAAA aetna Aen nea tad hehe an ann ah tn no nen a a a toler f te serol page TK4 1o sered 30 pages ENTER 1s est Figure 4 34 Insufficient liquid in WBC bath Figure 4 35 Insufficient liquid in RBC bath during count during count BC 2800 Auto Hematology Analyzer V1 1 4 7 Histograms and Pulse Graphs phticngsh 739909 camem poston 204800 TT N EEEE fJ te soroa page T11 to sarot 30 pages DITER io cut Figure 4 36 Insufficient liquid in RBC bath during PLT counting p kng 2233232 cune pon
48. ins call Mindray Customer Service Department or the distributor 7 2 18 Lyse Out Possible causes insufficient lyse or wrong lyse volume setting Solution Check if there is sufficient lyse left If so access Setup Settings gt Reagents and adjust the remaining lyse volume if not change a new container of lyse 7 2 19 Diluent Expired The diluent has expired or its expiration date is not correctly set Check whether the diluent has expired If so change a new container of diluent if not access Setup gt Settings Reagents and adjust the expiration date 7 2 20 Rinse Expired Possible causes rinse has expired or its expiration date is not correctly set Solution Check the rinse If it is expired change a new container of rinse if not access Setup Settings Reagents and adjust the expiration date 7 2 21 Lyse Expired The lyse has expired or its expiration date is not correctly set Check whether the lyse has expired If so change a new container of lyse if not access Setup Settings Reagents and adjust the expiration date 7 2 22 Filter Error Something is wrong with the filter of the vacuum chamber Solution Access Service Self test Tubing and test the filter The error is cleared if the result is normal If not call Mindray Customer Service Department or the distributor 7 2 23 Real Time Clock Error Something is wrong with the clock Access Setup Settings gt Date
49. ion Barcode Figure 6 25 Configuration Every screen displays 13 items and you can press t or to select the item you want to see or press PgUp or PgDn to go to the previous or next screen If you want to print out the configuration press PRINT If you want to acquire help press HELP BC 2800 Auto Hematology Analyzer V1 1 6 25 Maintenance 6 7 Printing Management Press the MENU to enter the system menu and press the appropriate arrow keys t i lt to move the cursor to Service Print as Figure 6 26 shows Software version 1 0 0 2 S N Ac 000000 Storage capacity 10000 gi Discriminator Displayed Enter sample into All into 6 Search On date Review Maintenance Status Quality contro Setup Self test Og Calibration Config Shutdown Figure 6 26 Entering print screen Press ENTER to enter the Print screen as Figure 6 27 shows The printing tasks are queued in this screen where you can view the all tasks and delete those waiting to be processed Once something goes wrong with the printing device the task being processed will be deleted and the queued tasks will keep waiting Once the system finds the error has been removed it will resume printing and process the tasks from the first one Note that you cannot change the sequence of the queued tasks Figure 6 27 Print s
50. ipping container Return shipments will not be accepted if the Mindray number is not clearly visible Please provide the model number serial number and a brief description of the reason for return 2 Freight policy The customer is responsible for freight charges when equipment is shipped to Mindray for service this includes customs charges Company Contact Address Mindray Building Keji 12th Road South Hi tech Industrial Park Nanshan Shenzhen P R China Phone 86 755 26582888 Fax 86 755 26582680 Authorized Representative Name Shanghai International Holding Corp GmbH Europe Address Eiffestra B e 80 D 20537 Hamburg Germany Phone 49 40 2513175 Fax 49 40 255726 IV BC 2800 Auto Hematology Analyzer V1 1 Content Content Chapter 1 Hardware secs asssgcssaanckeattagatseaannbarn bank akgensbace dean eas eanands aaa 1 1 LCL Generala r a E a E E E A A AE 1 1 k OW SI SUMO IY ih e eee deb ER e EE OK Eer KOEK E Eei 1 3 TI Oe ever e E E E E a 1 4 1 4 CPU and Peripheral DeVICES cciccicscsaiscivccsiaccinstietivecciedcteshetiieciatesecdices 1 5 1 5 Analog Inputs and Outputs cc ccssers catencceteee cance cate eater eee 1 8 1 6 Digital Inputs and Outputs sass vecks exci evsnznsiecaeenth essernevbeapentheosemmaveaomes ons 1 9 ET Driving Boarne e Vleet e ven en vice Rene vents ye Rel v een vier 1 11 1 8 Display Unitins ene wn weGnees OAR 1 14 1 9 Functions of the Keypad Adapter cc cceeeeeeeee
51. ith the communication between the scanner and the analyzer Solution Check the connection between the two devices 7 2 12 Printer Out of Paper Possible cause The printing paper has run out or is not correctly installed Solution Check whether the printer is out of paper If so load paper to the printer otherwise re install the existing paper If the error still remains please contact Mindray Customer Service Department or the distributor 7 2 13 Printer Connection Error Check whether the printer is well connected to the analyzer If the error still remains please contact Mindray Customer Service Department or the distributor 7 2 14 Recorder Communication Error Call Mindray Customer Service Department or the distributor 7 2 15 Recorder Out of Paper Possible causes recording paper has run out or is not correctly installed Solution Check whether the recording paper has run out If so load new paper if not re install the existing paper If the error still remains call Mindray Customer Service Department or the distributor 7 2 16 Recorder Too Hot Possible causes the recording head overheats 7 6 BC 2800 Auto Hematology Analyzer V1 1 Troubleshooting Solution Stop using the recorder If the error repeats call Mindray Customer Service Department or the distributor 7 2 17 Press Bar Up Possible causes The press bar of the recorder is up Solution Push it back If the error still rema
52. ixing plate of the power module As the figure below shows remove the retaining screws totally 10 to remove the plates BC 2800 Auto Hematology Analyzer V1 1 3 3 Disassembling the Analyzer Figure 3 5 3 2 3 Removing the front cover As the figure below shows remove the retaining screws totally 6 to remove the cover Figure3 6 3 4 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer 3 2 4 Removing the LCD assembly As the figure below shows remove the retaining screws totally 4 to remove the assembly Figure3 7 3 2 5 Removing the keypad As the figure below shows remove the retaining screws totally 7 to remove the keypad Figure3 8 3 2 6 Removing the LCD and adaptor As the figure below shows remove the retaining screws 4 for each part to remove the LCD and adaptor BC 2800 Auto Hematology Analyzer V1 1 3 5 Disassembling the Analyzer NN S i j AA bel Figure3 9 3 2 7 Removing the power shielding box As the figure below shows remove the retaining screws totally 3 to remove the box Figure3 10 3 2 8 Removing the driving board analog board CPU board and power board As the figure below shows remove the retaining screws totally 18 t
53. low the displayed instructions to remove all the tubes except for the one for the waste from this analyzer Press ENTER to start the draining procedure When the draining is done the screen will display Turn off this analyzer and you should turn off this analyzer as instructed 6 14 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 2 12 Cleaning Probe Wipe Block After being used for a long time the bottom of the probe wipe block may be contaminated by blood and the inside of the block may also be contaminated by the dirt sucked in So you need to clean the wipe block regularly Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys CJL I lt to move the cursor to Clean wipe block Present the probe cleanser to the sample probe and press ENTER to aspirate the cleanser Remove the cleanser after the probe has risen up Unscrew and remove the retaining screws with hands or screwdrivers pointed by the arrows shown in Figure 6 9 on the right plate of this analyzer Follow the arrow shown in Figure 6 10 to push and remove the right plate Follow the instructions displayed on the screen to place an empty cup below the sample probe Press ENTER to soak the wipe block with the aspirated cleanser The soaking progress will be displayed on the screen as Figure 6 14 Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture
54. nalysis This analyzer can process two types of blood samples whole blood samples and prediluted blood samples 2 5 1 1 Whole blood mode When analyzing a whole blood sample this analyzer aspirates 13 L of the sample and follow the procedure presented in Figure 2 10 to dilute it before proceeding to the actual analysis Whole Blood Sample 13uL Diluent 3mL 1 231 Dilution Diluent 0 5mL Diluent 3 5mL For WBC Analysis 1 308 Dilution For RBC Analysis 1 44862 Dilution Figure 2 10 Diluting procedure of whole blood mode 2 5 1 2 Prediluted mode When analyzing a prediluted sample the operator should first collect 20 u L of capillary sample and dispense 1 6mL of diluent from this analyzer to predilute the sample Then the operator should present the prediluted sample to the analyzer 2 12 BC 2800 Auto Hematology Analyzer V1 1 Fluidic System which will aspirate 0 7ml of the sample for further dilution as Figure 2 11 shows Capillary Blood Sample 20uL Diluent 1 6mL 1 1 Dilution Diluent 2 1mL 1 324 Dilution Diluent O0 44mL Lyse 0 36mL For WBC Analysis 1 417 Dilution Diluent 3mL For RBC Analysis 1 45004 Dilution Figure 2 11 Diluting procedure of prediluted mode 2 5 1 3 Volume range of blood cells After reacting with the diluent and lyse the cell volumes mainly fall into the following ranges WBC 30 350 fL RBC 25 25
55. nalyzer It helps the service engineers diagnose system errors Press MENU to enter the system menu and press the appropriate arrow keys t JL Ji lt to move the cursor to Service Log as Figure 6 23 shows Count Setup Review Quality contro gt Status Calibration Shutdown Tubing O Machine O Valve Circuit Maintenance gt Self test Config Print Reagents for gt 99 Counts enu A ITER Enter sut Figure 6 23 Entering log Press ENTER to enter the Log screen as Figure 6 24 shows O Other Settings NO Time Type g g Times Informat ion 1 1 04 2005 15 34 Self test 2 09 04 2005 15 30 Others 3 09 04 2005 15 23 Print amp comm 4 03 04 2005 15 21 Self test 2 MENU F 1 Select log type T 1 PgUp PgOn Scrol Figure 6 24 Log screen BC 2800 Auto Hematology Analyzer V1 1 6 23 Maintenance The recorded events are divided into three groups All Settings and Other including setting discriminators system self test and updating system software which are all listed on the left of the screen All the recorded events are listed on the right of the screen by default You can press F1 to select the interested group and the right of the screen will display the events of the selected group only Every screen displays 10 events You can press ft or J
56. needed When you think the bath might be dirty perform the Clean the bath procedure When the analyzed samples add up to 200 the system will remind you to perform the Probe cleanser cleaning procedure When the analyzed samples add up to 4 000 the system will remind you to perform the Clean wipe block procedure When this analyzer is not to be used for two weeks be sure to perform the Prepare to ship procedure to empty and wash the fluidic system To obtain reliable analysis results this analyzer needs to work in a normal status Be sure to run the Self test items regularly to check the status of this analyzer When this analyzer gives alarms for clogging you can perform the Flush aperture or Zap aperture procedure or press F2 at the Count screen to unclog the system If you see other error messages see Chapter 7 Troubleshooting for solutions BC 2800 Auto Hematology Analyzer V1 1 6 3 Maintenance 6 2 System Maintenance Press MENU to enter the system menu and press the appropriate arrow keys t JL 4 J lt to move the cursor to Service Maintenance as Figure 6 1 shows Whole Blood AIl Current sample WBC H 12 5 x 10L Lymph H 48x10 X Mid H 10x10 W 0 120 Gran 6 7 x 10L Time 01 27 2004 22 23 Lymph 39 0 x WD Mid 8 1 A CaA 4 an Mode WB All i 100 200 300 fL i j Gran 53 9 HGR H 167gL Name gt R RAC awx wn R HOT
57. ng Lyse entrance Lyse Diluent dispensing Sample dispensing tube tube probe E Diluent entrance Ly Syringe assembly Figure 2 3 Aspiration dispensing module The lyse is aspirated and dispensed into the bath as described below The valves 1 3 and 5 are open and the motor pulls the syringe plunger downward to aspirate certain amount of lyse The aspirated lyse is stored in the buffer tubing of valves 1 and 3 Then valve 1 is closed and the motor runs reversely to push the syringe plunger upward to dispense the stored lyse into the bath Since the capacity of the buffer tubing is far greater than the volume of the aspirated lyse the lyse will not overflow to the syringes through valves 3 and 4 Service tip Note that the length and type of the buffer tubing shall not be changed 2 4 2 Counting module The counting module mainly consists of the bath valve 1 and 2 vacuum filter metering tube negative pressure and other supporting components as figure below shows BC 2800 Auto Hematology Analyzer V1 1 2 5 Fluidic System i r ye r x ar dust ra ll 2 ilter eee tis sssi N Io o l l l Metering tube l l Aperture Negative Pressure Front chamber bath Back bath Figure2 4 Counting module The counting module is implements the most important function of the analyzer counting The electrodes installed on the bath detect the pulses caused by blood c
58. ng the Lyse Test procedure to expose the bath BC 2800 Auto Hematology Analyzer V1 1 6 13 Maintenance At the Maintenance screen press the appropriate arrow keys t lt to move the cursor to Drain bath Press ENTER to start the procedure and the screen will display the progress bar When the bath is drained the screen displays Drain bath as Figure 6 13 shows Check the bath and its lower hose for residual liquid If there is turn off the analyzer and call the Mindray Customer Service Department or the distributor for repair if not press ENTER and the analyzer will prime the bath with diluent When the priming is done the screen will return to the initial state and you should try to find other reasons the contribute to the abnormal results tem Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe black Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Prime the bath with diluent Press ENTER to continue Select service Figure6 13 Drain Bath 6 2 11 Draining Tubing If this analyzer is not to be used for a long time or it is to be maintained be sure to perform the Drain tubing procedure Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys CIL I lt to move the cursor to Drain tubing Press ENTER and fol
59. o remove the boards 3 6 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer Figure3 11 3 2 9 Removing the transformer As the figure below shows remove the retaining screws totally 2 with a socket wrench Figure3 12 3 2 10 Removing valves As the figure below shows remove the retaining screws two for each valve to remove the valves BC 2800 Auto Hematology Analyzer V1 1 3 7 Disassembling the Analyzer Figure3 13 3 2 11 Removing the sample probe assembly As the figure below shows remove the retaining screws totally 3 to remove the assembly SoZ Figure3 14 3 8 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer 3 2 12 Removing the syringe assembly As the figure below shows remove the retaining screws totally 4 to remove the assembly Figure3 15 3 2 13 Removing the vacuum chamber and volumetric metering tube assembly As the figure below shows remove the retaining screws 4 for each part to remove the assembly BC 2800 Auto Hematology Analyzer V1 1 3 9 Disassembling the Analyzer i _ re F z S 7 5 Figure3 16 3 2 14 Adjusting Sample Probe and Replacing Wipe block The relative position between the sample probe and wipe block has influence on the test results In the accessory kit there is a sample probe localizer as Figure 8 28 shows You need to use the localizer to adjust the position of the s
60. o tubing malfunctions Therefore testing the valves is a major way to remove fluidic errors At the Self test screen press F1 to select the Valve group as Figure 6 21 shows The following test items will be displayed on the right of the screen Press the appropriate arrow keys t lt to select the valve you want to check and press ENTER to test it If the valve goes through the Off On Off sequence without making abnormal sound it passes the test Otherwise something may be wrong with the valve Tubing item Result I item Result O Machine vi i Off V7 Off Oo vi be Valve 7 ka fE Circuit in i pil v3 Off yo Off WA Off vid Off v5 Oft vii ort Select test ENTER D Figure 6 21 Testing valves 6 4 4 Testing Circuits At the Self test screen press F1 to select the Circuit group as Figure 6 22 shows You can test the A D interrupt at this screen by pressing ENTER to see whether the WBC RBC and PLT signals can be properly converted into digital signals BC 2800 Auto Hematology Analyzer V1 1 6 21 Maintenance Tubing tem Result O Machine O Valve Circuit Reagents for Select test ENTER Execute Figure 6 22 Testing A D converter 6 22 BC 2800 Auto Hematology Analyzer V1 1 Maintenance 6 5 Log The log records all the major events taking place during the running of this a
61. omer Service Department or the distributor HGB Error HGB gain is not correct and HGB blank voltage is within OV 3 2V or 4 9V 5V Solution Access Setup Password to gain the administrator authority Access Setup gt Settings Gain to adjust the HGB blank voltage to 3 4 4 8V 4 5V recommended If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor HGB Adjustment HGB gain is not correct and HGB blank voltage is within 3 2 3 4V or 4 8 4 9V Solution Access Setup Password to gain the administrator authority Access Setup gt Settings Gain to adjust the HGB blank voltage to 3 4 4 8V 4 5V recommended If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor WBC Clog This error message occurs when the actual WBC count time is greater than the preset WBC count time by 2 seconds BC 2800 Auto Hematology Analyzer V1 1 7 3 Troubleshooting Possible causes clogged aperture inappropriate WBC count time settings or solenoid valve error Solution Access Service Maintenance and do the Zap aperture and Flush aperture procedures After unclogging access Setup Settings Count to note down the preset WBC count time Access Service Self test Tubing and test the actual WBC count time If the tested time differs from the preset time by less than 2 seconds it means th
62. or other Mindray analyzer Only the J2 is installed for the BC 2800 The J1 serves to connect the LCD signal cable 1 14 BC 2800 Auto Hematology Analyzer V1 1 Hardware For new BC 3000 For BC 2800 Figure 1 10 Schematic of the adapter BC 2800 Auto Hematology Analyzer V1 1 1 15 Hardware 1 9 Functions of the Keypad Adapter 1 To scan the keypad The keypad adapter scans the keypad and reports the scanned key code to the main board 2 To control the LCD brightness The keypad adapter receives the instructions from the main board to turn on off the backlights and power indicator of the LCD and to control the brightness of the backlights 3 To control the buzzer The keypad adapter receives the instructions from the main board to turn on off the buzzer 1 9 1 Architecture of the Adapter The adapter mainly consists of a MCU keypad matrix backlight control power indicator control and buzzer 1 9 2 Detailed Description 1 9 2 1 Power supply The main board provides a 12V and 3 3V supplies which are isolated from each other The 3 3V supply is the main power of the adapter and the 12V is passed to the backlight board inverter of the LCD and also converted to a 5V supply to drive the buzzer and controls the on off of the backlight power the adapter Since the 3 3V and 12V are isolated the MCU send the control signals to the buzzer and backlight board through photocouplers 1 9 2 2 MCU The MCU is AT89C
63. ory attire including rubber gloves and follow safe laboratory procedures when handling any material in the laboratory You can soak the bath and tubing with the probe cleanser an alkaline detergent by performing the Probe cleanser cleaning procedure If your analyzer is to run 24 hours a day you should perform this procedure every 3 days If you follow the shutdown procedure to turn off your analyzer everyday you should perform this procedure every week Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys t J lt to move the cursor to Probe cleanser cleaning Present the cleanser to the probe and press ENTER to aspirate the cleanser Remove the cleanser after the probe has risen up This analyzer will automatically soak the bath and tubing with the aspirated cleanser The soaking process will last about 5 minutes and you may press ENTER to stop the process any time Note that a shortened soaking process may not be as effective as a complete one BC 2800 Auto Hematology Analyzer V1 1 6 9 Maintenance When the soaking is done press ENTER to flush the bath and tubing after which screen will return to the initial state To make sure this analyzer functions normally every time the accumulated analyzed samples reach 200 a dialog box will pop up to remind you to perform the Probe cleanser cleaning procedure as Figure 6 8 shows If you want to do
64. per communication error error abnormal R d t of R d t 1004 ecorder out o ecorder o0 1006 Press bar up paper hot 2004 Lyse expired Filter error 2006 Real time clock error Syringe motor Rotation motor 4004 Elevator motor error error error 400B Diluent out Rinse out 400D Waste full f Dynamic 8001 File error memory error 7 2 BC 2800 Auto Hematology Analyzer V1 1 Troubleshooting 7 2 Solutions 7 2 1 7 2 2 7 2 3 7 2 4 7 2 5 This chapter presents measures to be taken when the errors occur Environmental Temperature Abnormal Possible causes abnormal environmental temperature or malfunctioning temperature sensor Solution Access Service Status and check the environmental temperature If the temperature exceeds the specified range by 15 C 35 C you need to adjust the work environment of this analyzer so that the analyzer works in the requested environment If the temperature is within the requested range and the error remains call Mindray Customer Service Department or the distributor Background Abnormal At least one parameter failed the background check Solution At the Count screen press F3 to do the startup procedure If the error still remains access Service Maintenance and do the Probe cleanser cleaning procedure When the cleaning is done return to the Count screen and check the background again to see whether the error is cleared If not call Mindray Cust
65. pter6 Maintenance Regular cleaning and maintenance are demanded to guarantee this analyzer operating properly This chapter introduces how to take care of this analyzer and check the system status Liquid overflow or leak during the operation of this analyzer will degrade the accuracy of the analysis results Once it occurs immediately wipe off the spills If it occurs inside this analyzer be sure to shut down the power immediately and call Mindray Customer Services Department or the distributor Otherwise the service life of this analyzer may be shortened BC 2800 Auto Hematology Analyzer V1 1 6 1 Maintenance 6 1 Regular Maintenance To keep this analyzer in a good shape regular maintenance is demanded 6 1 1 Special Notes The parts in contact with blood are potentially infectious Be sure to wear standard laboratory attire including rubber gloves when maintaining or operating this analyzer and wash your hands with detergent when you are done Be sure to keep you hair clothes cuff or hands away from the moving parts of this analyzer Be sure to use specified tools or parts to maintain this analyzer and be sure to clean the used tools as instructed by their instruction manual when you are done Be sure to use soft and clean cloth or neutral detergent soaked cloth twisted dry or soft cloth washed by ethanol to clean the surface of this analyzer Be sure to pay attention to the marks or symbols on this analyzer
66. r the distributor 7 2 34 Dynamic Memory Error Something is wrong with the system memory Turn off the analyzer and call Mindray Customer Service Department or the distributor BC 2800 Auto Hematology Analyzer V1 1 7 9 List of Spare parts Chapters List of Spare parts P N Description 0000 10 10891 Keyboard 0000 10 10907 Disk on module 32M 0010 10 12316 Hitachi STN screen 640X480 0200 10 05528 Bearing for syringes 0200 20 05530 Plunger 1800 30 19455 Indicator board 2000 20 03124 Ruby Red Cell Counter 80um RB 22084 2800 20 32054 START key 2800 30 32057 Recorder assembly 2800 20 28674 Keypad overlay 2800 20 28696 Syringe press plate 2800 20 28699 Plunger pin 2800 20 28700 7 5ml bolt 2800 20 28701 Upper shielding plate for the analog board 2800 20 28702 Lower shielding plate for the analog board 2800 20 28719 Guide bushing 2800 20 28724 Sealing bushing 2800 20 28725 Sealing ring 2800 20 28739 Connection wires for the indicator board 2800 20 28740 LCD adaptor wire flexible FFC 2800 20 28754 2800 20 28757 Connection wire between the keypad and the recorder Connection wire of the position sensor 2800 20 28770 Connection wire for the LCD 2800 20 28843 Body of 7 5ml syringe 2800 20 28844 Flange of 7 5ml syringe 2800 20 28
67. red in the FLASH so the FLASH is also called the BootROM Every time the system is powered on the CPU first executes the boot program that initializes the system and loads the control software from the DOM The FLASH also contains such information as the FPGA configuration FPGA version and LCD contrast 1 4 4 SDRAM The system memory consists of two 8M byte memories 1 4 5 DOM The CPU board uses a 32M DOM that is powered by a 3 3V supply the DOM can also be supplied by 5V supply The DOM is only operational after the FPGA is configured 1 4 6 RTC The CPU board uses a real time clock RTC to record the time The RTC is connected to the I C bus of the CPU board and synchronized by a 32 768KHz crystal oscillator When the analyzer is powered on the RTC is powered by the CPU board when the analyzer is powered off it is powered by the built in battery 1 4 7 EEPROM The CPU board uses an 8K byte EEPROM to store such information as system configurations and settings It is connected to the I2C bus of the CPU and can be written by CPU on line 1 4 8 LEDs When D1 is on it means 3 3V is functioning properly When D9 is on it means 12 8V is functioning properly When D5 is on it means the system is 1 6 BC 2800 Auto Hematology Analyzer V1 1 Hardware reading or writing the DOM When D7 is on it means the FPGA has been configured and is functioning properly When D20 is on it means the FPGA is restarting The D11 D1
68. sary contact with the probe and probe area Follow the steps given below to replace the wipe block 1 Follow steps 1 6 according to Section 3 3 1 2 Pull the loosen sample probe upward until it out of the wipe block then remove the wipe block and disconnect the tubings from the it pay attention to the correspondence between the tubings and the connectors as the figure below shows Install a new wipe block and connect the tubings the tubing marked with a black line should connect to the bottom connector When the connection is done place the wipe block back to its original position 3 Follow steps 7 9 according to Section 3 3 1 to fix the sample probe 3 14 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer Figure3 24 Installing probe wipe BC 2800 Auto Hematology Analyzer V1 1 3 15 Histograms and Pulse Graphs Chapter4 Histograms and Pulse Graphs 4 1 Histograms This chapter presents several WBC histograms you may encounter frequently 1 A normal histogram a G d 0 50 100 150 200 250 300 fL Figure 4 1 NOTE Blood cells lain between the first and the second discriminators are lymphocyte those between the second and the third discriminators are mid sized cells those between the third and the fourth discriminators are granulocyte The fourth discriminator is the fixed line 2 A histogram that is too narrow to indicate the differential result
69. the liquid and air in the pressure chamber is expelled to the outside through the NO end of valve 1 and the pump and the NC end of valve 2 to establish the vacuum The pressure value is monitored by the pressure sensor The pump is an imported American product whose P N is 3001 10 07252 BC 2800 Auto Hematology Analyzer V1 1 2 9 Fluidic System Pressure Pressure sensor sensor lt Probe Wipe Le Negative pressure Negative pressure chamber chamber NO Waste exit gt Waste exit yc gt Pump AY NC Probe Wipe NC Figure2 7 Pressure vacuum module the left establishes the pressure the right establishes the vacuum 2 4 5 Mixing module The types of mixing is available mixing by the horizontal movement of the sample probe and bubble mixing As Figure2 8 shows the aspirated sample needs to be diluted before the counting Once the sample is dispensed into the bath containing certain amount of dilute the system will inform the mixing module to work Then the sample probe initiates a mild horizontal movement inside the bath and the positive pressure is established inside the pressure chamber The valve is then open briefly and separate the air in the pressure chamber into several air segments and expel them into the bath to introduces bubbles The bubbles pop
70. to Hematology Analyzer V1 1 3 11 Disassembling the Analyzer Tubing Item Result Machine Syringe motor O Valve O Circuit Rotation motor Elevator motor Print Reagents for Select test ENTER Execute Figure3 19 Elevator motor screen Press f to move the sample probe upward and press to move the probe to the top of the bath as the figure below shows Figure 3 20 Sample probe on the top of the bath 3 Loose the retaining screw by a Philips screwdriver as the figure below shows 3 12 BC 2800 Auto Hematology Analyzer V1 1 Disassembling the Analyzer Figure 3 21 Removing screws 4 Remove the probe from the wipe block and insert the localizer into the wipe block from the bottom as the figure below shows N Figure3 22 Insert localizer 5 Insert the probe into the wipe block until it reaches the localizer as the figure below shows BC 2800 Auto Hematology Analyzer V1 1 3 13 Disassembling the Analyzer Figure3 23 Inserting sample probe into wipe block 6 Re tighten the screw to fix the probe and remove the localizer 3 2 16 Replacing Wipe block Biohazard Wear standard laboratory attire including rubber gloves and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unneces
71. ts 2 UART 3 3VTTL one to connect the keypad and the other reserved to control the pump Another 2 UARTs RS232 are implemented inside the Super I O to connect the scanner and to communicate with the PC 1 6 2 Parallel Port and PS 2 Port The Super I O provides a DB25 parallel connector to connect to connect a printer or a floppy drive the power supply of the floppy drive is supplied by the PS 2 The software will automatically adapt to the connected printer or the floppy drive The Super I O provides a keyboard interface and a mouse interface COM3 and COM4 Note that the BC 2800 does not support the mouse yet 1 6 3 GPIOs 1 Signals of the Start key The FPGA detects the input signal which will turn low when the Start key is pressed 2 Volumetric metering Signals The FPGA detects the signals sent by the start transducer and the end transducer BC 2800 Auto Hematology Analyzer V1 1 1 9 Hardware Signals of level detection The BC 2800 has not level sensors Digital pot The SPI bus interface implemented by the FPGA controls the 4 digital potential meters on the analog board to control the HGB gain Signals controlling valves and pumps The Super I O outputs 20 control signals to control the valves and pumps through the driving board Since the BC 2800 only has 1 pump and 11 valves the redundant lines and ports are reserved Signals controlling bath The Super I O outputs 4 control signals through the analog board
72. ule The flushing module includes washing the exterior and interior of the sample probe washing the front and back bath flushing the aperture and flushing the fluidic lines 2 4 3 1 Washing exterior and interior of sample probe The part that washes the sample probe is shown in Figure2 5 The washing consists of two procedures washing the exterior and washing the interior 1 To wash the exterior The plunger of the 10mL syringe moves downward to aspirate diluent from the NO end of valve 6 Then the plunger moves upward and valves 6 and 5 are open so that the aspirated diluent is dispensed through the NC end of valve 6 NC end of valve 5 and valves 4 and 3 to the lower part of the probe wipe Then the pump functions to open valves 9 and 10 to introduce a negative pressure on the upper part of the probe wipe Because of this negative pressure the diluent dispensed to the lower part of the probe wipe is transferred to the upper part and washes the exterior through the up and down movement of the sample probe 2 To wash the interior The plunger of the 10mL syringe moves downward to aspirate diluent from the NO end of valve 6 Then the plunger moves upward to dispense the aspirated diluent through valves 5 and 6 and the buffer tubing to the sample probe to wash the interior BC 2800 Auto Hematology Analyzer V1 1 2 7 Fluidic System
73. unt time If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor 7 2 7 RBC Clog This error message occurs when the actual RBC count time is greater than the 7 4 BC 2800 Auto Hematology Analyzer V1 1 Troubleshooting 7 2 8 preset RBC count time by 2 seconds Possible causes clogged aperture inappropriate RBC count time settings or solenoid valve error Solution Access Service Maintenance and do the Zap aperture and Flush aperture procedures After unclogging access Setup Settings Count to note down the preset RBC count time Access Service Self test Tubing and test the actual RBC count time If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis Other wise Access Service Maintenance to soak the bath and tubing with probe cleanser When the soaking is done access Setup Settings Count to note down the preset RBC count time Access Service Self test Tubing and test the actual RBC count time If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis If the difference is still greater than 2 seconds and stabilized around a certain value access Setup gt Settings Count to change the RBC count tim
74. ure BC 2800 Auto Hematology Analyzer V1 1 6 19 Maintenance Filter It checks whether the filter functions normally 6 4 2 Testing Motors and Recorder Printer At the Self test screen press F1 to select the Machine group as Figure 6 20 shows The following test items will be displayed on the right of the screen Press t or to select the desired item and press ENTER to conduct the test Tubing tem Result Machine E Syringe motor O Valve Circuit Rotation motor Elevator motor Print Select test ENTER Execute Figure 6 20 Testing mechanic parts Syringe motor The syringe motor controls the aspiration volume This test checks whether the motor functions normally Rotation motor The rotation motor rotates the sample probe inside the analyzer This test checks whether the motor functions normally Elevator motor The elevator motor controls elevation of the sample probe This test checks whether the motor functions normally Print This test checks whether the recorder or printer functions normally If normal when you press ENTER the recorder or printer will print out test page if abnormal the screen will display the corresponding error message and you can 6 20 BC 2800 Auto Hematology Analyzer V1 1 Maintenance see Chapter 7 Troubleshooting for solutions 6 4 3 Testing Valves Malfunctioning valves will lead t

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