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Binder CB CO2 broedstoof Service manual ENG

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2. 38 Reading out OF the actual Values EE 39 zip AA TUE OE ANA AA AA TT SEE AG 39 Result Calibration alignment of the temperature controller 39 O gt Calibration e leo E EE 40 eee etri PNP T m mmm di FOE Ma E e EE 48 8 Explosion Drawings of the CB 2 en menn ene nemen serra renis Di Sil CB COMPONENT BOAO ME Di 8 2 CB Kettle Sensors Heaters Permad ry nemen 52 8 3 CB Door Sealing Inner parts 53 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger T Modification levels The CB CO Incubator E2 is a further development of CB CO Incubator E Especially the Display controller MB inside the red triangle is conspicuous The whole electronic is placed inside the lower part of the CB CO Incubator Front Access Maintenance Theres no Fbox as at the CB CO Incubator E at the top of the chamber EINDER P 37 0 36 8 5 0 49 25 0 25 2 gt Display Controller MI Machine Room with all electronic parts 2 Unit overview O LAN gt d EX N EN Wy N M L K A Displ
3. d giir 6 3 Tolerance of the adjustment Binder calibrates each chamber before it is dispatched to the customer High quality measuring systems are used for this whose accuracy is tested annually To guarantee best possible quality of results which are worked out by use of the CB incubator the controlled values should be checked annually and re calibrated if necessary When is it worth to correct a deviation between the value measured and the value displayed on the MDDI controller The following table gives you the conditions for an ideal adjustment of a temperature deviation Measured value Measured range 2 points adjustment is higher than Temperature Up to 60 C 37 C 29 point not used 0 3K CO volume in CO volume in O Vol and 5 Vol CO2 0 3 Vol CO Deviations which are smaller than this deviations do not require an adjustment Important The values on the above chart can only be used if the value of measurement uncertainty on the calibration certificate of the measuring instrument is smaller than the values of the chart When this is not the case then you must take as limit value for an adjustment the measurement uncertainty value of the calibration certificate 36 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Calibration instructions for CO2 incubator CB with screen controller MBI Temperature CO2 02 controller Measurement device for
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5. Enter the value 0 to Start Value W Enter the value 7000 to End Value X Enter the value 7000 to End Value W Set State to Off gt leave menu with 4 x EXIT Determination and entry of the calibration values The incubator has to be pre heated at the working temperature common value 37 C for at least half an hour The altitude of the site above the sea level has to be entered to the temperature CO controller The procedure is described in chapter 7 3 of the operation manual Afterwards the incubator is switched off at the main switch 7 and the CO sensor is pulled out of the incubator The unit doors can now remain open during the calibration The white filter of the CO sensor is removed and the original BINDER calibration cap is pushed on the sensor tip The sensor with the calibration cup is put into the socket of the incubator again and the incubator is switch on at the main switch CT The calibration is carried out in two individual steps At first the sensor is exposed to pure nitrogen and afterwards it is exposed to analysed CO test gas with known CO concentration The original BINDER one litre pressure gas bottles T0bar have to be used The test gas bottles are connected via the original BINDER pressure reducer with flow meter and a plastic tube with the calibration cup The flow rate has to be situated between 600 and 900 ml min This is equal to the two highest graduations of the flow meter in upright position
6. Version of the described Chamber Standard equipped CB CO Incubator E2 with FPI Sensor System and MB Controller SERIAL NO O 270Ob4 gt Order No 9040 002 CB IAU Order No 90940 003 CB 20 Contents ii Modification 6 8 5 io nee TM 3 Bue rv ror ip ne RN EN a ENG 54855545558 FEA KENA a ENG e 4 2 NGORONG IEEE 5 2 2 Short description of the MB Controller aaa aaa eaaa eaaa aaa anana eaaa anae mr 6 S FUNCIO AA E AAAH ES ESE ETE I3 Sil The UO Measuring be leet I73 3 2 Function of the Heating System meme nee enne seen nes I73 3 3 Flow Chart of the heating function basis CB ID0 wiring dagram aee I7 3 4 Controller MB PIN description Input Oufmput IA 3 5 Function of the CO System KREE sese sese sese sen sena US 3 6 Flow Chart of the CO System basis CB wiring dagram US 37 Puneclon orihe PermadryB E E Ip 3 8 Flow Chart of the Permadry system basis CB wiring diagram I4 3 9 Description of the Function of the Fan Control I4 270 eniz eler Oe EE I8 ENK PORAN SLOT ZAU ON a ANAN AANGAL I8 AI2 Hot air sterilizati
7. 20 d O 2 8 4 28 28 o i8 28 M Tron Module Option N O Control GO A O0 70 M Tron A070 SOT A O07I M Tron 4030 O A 22 bi state OTI 20072 created 03 2002 Jochen Tussinger Description Rear Wall Main Fan Motor Part Insulation Kettle Heating Element D7EV 550W left Pri 00 temperature probe heating Element M Ar icde NOo 005 with wi 52 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 8 3 CB Door Sealing Inner parts Number Description Ar cle No Is mounted on Glass Door Hinge Glass Door Aa Inner Part CB Door By reguest i 8005 0080 C8 270 6005 0080 CB 240 By request GOOA 0070 CB 270 4005 0094 CB 270 4008009 CB 20 4005 0095 CB 270 Housing Cover AO0 02096 CB IO ti PET AA Cover Front Access Module 4022 0054 CB jIb0 4022 0077 CB 200 6002 0060 GOUT O00Z0U CB Z70 6005 0080 GEAR MN 9 50 bi 4 5 lt lt ee 5
8. Enter the value 7000 to End Value X Enter the value 7000 to End Value W Set State to Off EXIT Analogue input 2 Enter the value 0 to Start Value X Enter the value 0 to Start Value W Enter the value 7000 to End Value X Enter the value 7000 to End Value W Set State to Off EXIT Safety controller Enter the value 0 to Start Value X Enter the value 0 to Start Value W Enter the value 7000 to End Value X Enter the value 7000 to End Value W Set State to Off leave menu with 4 x EXIT 38 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Entry of the reference temperature Starting Situation During T hour without door opening the incubator is adjusted stable to the calibration temperature e g X W 37 C The reference instrument shows e g 36 5 C Reading out of the actual values Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Analogue Inputs read out and note and 2 leave the menu with 3 x EXIT Normal Display User Settings read out and note Safety control Act leave the menu with EXIT Example Reference device Analogue Input 7 equals Analogue Input 2 Safety control Act value X value W in Normal display 36 5 C 37 0 C 36 7 C 36 9 C Entries Normal Display Config Configuration 2 gt System Code 2802 Service Level gt Inp Val correct Analogue input 7 Enter
9. With the standard hot air sterilization at I8 br it is absolutely guaranteed that all germs are reliably eliminated and furthermore also time is saved All unit parts in the inner chamber are auto sterilized at a temperature of I8 Dnt The precision IR sensor is separately treated in a desinfectant bath This ensures that the measuring quality does not suffer from the high temperature as it may happen with other systems 2 I Hot air sterilization The hot air sterilization gets activated in the CB Menu The sterilization temperature of 87 5 C is adjusted by the manufacturer It may not be changed The CO sensor is temperature resistant up to a maximum temperature of 60 C By no means it can be sterilized or autoclaved The CO5 sensor head is especially adjusted for a specific chamber that it belongs to It cannot be operated in another chamber There is an adhesive label with a number on the sensor head to avoid any mixing up Effect the hot air sterilization as following TI Switch off the unit 2 Pull out the COs sensor without rotating from the connection bushing in the upper part of the rear and remove it from the inner chamber Only with option Oxygen control Remove the oxygen sensor from the connection bushing without rotating and put it on the middle shelf Before starting the sterilization please remove the water from the basin Shut the outer and the inner door of the unit Ensure that the water basins the shelf h
10. 2 25 3 254 25 5 25 6 25 7 25 8 25 9 26 ZD 26 2 26 3 26 4 26 5 26 6 26 7 26 8 26 9 27 27 27 3 27 4 27 5 27 6 27 7 27 8 27 9 28 CN 28 2 28 3 28 4 28 5 28 6 28 7 28 8 28 9 29 20 i 29 3 29 4 29 5 29 6 29 7 29 8 29 9 0 SA 30 2 30 3 30 4 30 5 30 6 30 7 30 8 30 9 3i 3I 3 2 Sab 3i 4 Su 37 6 2A7I ZAI8 ZAI8 32 SCH 32 2 32 3 32 4 32 5 32 6 32 7 32 8 32 9 33 AA 33 2 33 4 33 5 33 6 33 7 33 8 33 9 34 24 34 2 34 3 34 4 34 5 34 6 34 7 34 8 34 9 5 A3 35 2 35 3 35 4 35 5 35 6 35 7 35 8 35 9 36 Sch 36 2 36 3 36 4 36 5 36 6 36 7 36 8 36 9 37 ETA Se 37 3 37 4 37 5 37 6 37 7 37 8 37 9 38 SC 38 2 38 3 38 4 38 5 38 6 38 7 38 8 38 9 39 39 39 2 39 3 39 4 39 5 39 6 39 7 39 8 39 9 0 A0 40 2 40 3 40 4 40 5 40 6 40 7 40 8 40 9 di SA SA SA d A SAS S z SA SA SA 42 SC 42 2 42 3 42 4 42 5 42 6 42 7 42 8 42 9 43 A3 43 2 43 3 43 4 43 5 43 6 43 7 43 8 43 9 44 44 44 2 44 3 444 44 5 44 6 44 7 44 8 44 9 5 45 45 2 45 3 45 4 45 5 45 6 45 7 45 8 45 9 46 dp 46 2 46 3 46 4 46 5 46 6 46 7 46 8 46 9 47 4TA 47 2 47 3 47 4 47 5 47 6 47 7 47 8 47 9 48 Ap 48 2 48 3 48 4 48 5 48 6 48 7 48 8 48 9 49 SC 49 2 49 3 49 4 49 5 49 6 49 7 49 8 49 9 45 CB 2 Service Manual O2 Vol U mV O2 Vol U mV O2 Vol U mV
11. 878 7 67 7 IO06G0 72 7 I7D65 52 8 668 9 57 8 768 7 62 8 SA 67 8 IO00 72 8 I7G0 0 52 9 670 8 57 9 770 8 62 9 883 5 67 9 IO0 I2b 72 9 MISS 53 672 7 58 772 9 63 885 9 68 IO0ID 2 73 I7G66p 53 674 6 D I gol SEN 888 3 D I IO0I8 0 3 I7G0 0 53 2 676 5 58 2 777 2 63 2 890 7 68 2 IO020 8 73 2 KGR 539 678 4 58 3 779 3 63 3 SEN 68 3 IO02236 73 3 I7 6G6 53 4 680 3 58 4 787 63 4 895 6 68 4 IO26G A 73 4 I7 00 53 5 682 3 58 5 783 6 63 5 898 0 68 5 I0207 73 5 RE Se 53 6 684 2 58 6 785 8 63 6 900 4 68 6 IO0227 73 6 I7I86pp SENG DOG 58 7 787 9 63 7 902 9 68 7 IO024 0 Ju I7900 53 8 688 0 58 8 ZOO 63 8 905 4 68 8 IO02 8 73 8 I702A 539 690 0 58 9 792 2 63 9 907 8 68 9 IO40 4 73 9 II06G8 54 GOI 0 59 794 4 64 Ma 69 IO43b 74 I200 7 54 693 8 DO 796 6 64 OiI28 SC IO46 A 74A TOU 54 2 695 8 59 2 798 8 64 2 MER 69 2 IO0407 74 2 I20 7 54 3 697 7 odis DOT 0 64 3 OI 69 3 UE 74 3 I2I06 54 4 699 7 59 4 803 64 4 920 2 69 4 IODD7 744 I274I 54 5 2078 59 5 805 3 64 5 922 8 69 5 IOD8 0 74 5 Mel 54 6 703 6 59 6 807 5 64 6 925 3 69 6 IO 9 74 6 I227 0 54 7 705 6 59 7 809 8 64 7 927 8 69 7 IO0G239 74 7 I224 6 54 8 707 5 59 8 OI20 64 8 930 3 69 8 IOpGp 8 74 8 UE 54 9 709 5 59 9 SE 64 9 932 8 69 9 IO 8 74 9 I227I6 46 5 37 75 2 75 3 75 4 T9 75 6 Pe 75 8 75 9 76 Z I 76 2 76 3 76 4 76 5 76 6 76 7 76 8 76 9 77 Fr 77 2 77 3 77 4 77 5 77 6 TI 77 8 ITA 78 793 78 2 78 3 78 4 78 5 78 6 78 7 78 8 78 9 79 79 3 79 2 79 3 794 9
12. Ge 9900 je gt S EE 0 6 N3dO 000 N3dO 5000 0 e o fs gt gt gt 5 le PISI pas Y ploy anja ji o o Ay uJgelv XA 9JON jue WI Ju kas uonejejsui Buuojiuow jeus 0 OJ U00 ay Aq e 0 abessaw uueje eu USULI 0 paya ims SI JOBJUOI WJe e 99 jenuajod LAWN 900 4 O e pue spunos Jezznq e awin 99 0849 0 e Buunp 2990 zou si uonipuoo Ang BAI DID D D IISIP 9 QISSOd e 959 U U99J9S 9 UO uly eniq e se jsJi ye eouequnisip Aue sojeoipul SUOHEIIPU WEE pue 59 0 JI9U UJIM se uegun wejsAs sisouDeip ojne siuojjoaja juabijjaju ue ulm peddinbe si e2iMep siu SIL aaa AACN pao CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 3 Function Incubators series CB are suited for cultivation of mammal cells under typical conditions of about 37 C The incubator allows the set defined pH conditions by justifying NaHCO buffer systems of commercial cell media by keeping exact CO atmosphere inside CB incubators care for high humidity inside to avoid increasing of the osmolarity caused by evaporation of the cell media To reach this the different functions heating sterilization and CO injection have to work as a perfect team This know how is as Firmware inside the controller type MI 3 4 The CO measuring principle The CO measuring procedure of the incubator series CB is characterized by fast reaction times as wel
13. Note The BINDER service uses larger pressure gas bottles with analysed test gases and an other flow meter But the procedure remains as described After 5 minutes exposing of the sensor to each test gas the CO2 concentration of the gas is entered to the MB controller according to the following description Exposion to Nitrogen 0 vol CO for 5 minutes Normal Display Config Configuration 2 System Code 2802 Service Level gt Inp Val correct gt Analogue input 4 Enter the display value of the controller e g 0 2 Vol 96 to Start Value X Enter the CO concentration of Nitrogen 0 Vol 96 to Start Value W leave menu with 4 x EXIT and stop exposure with Nitrogen 40 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Exposition to test gas 5 CO for 5 minutes Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct gt Analogue input 4 Enter the display value of the controller e g 4 7 Vol 96 to End Value X Enter the CO concentration of the CO test gas 5 0 Vol 96 to End Value W Set State to On gt leave menu with 4 x EXIT The corrected value 5 Vol appears on the controller display after 5 seconds Afterwards the exposure with test gas is stopped The analysis result of the test gas acc to the label of the gas bottle is reached 02 calibration alignment Reset of former cal
14. carried out for a comprehensive maintenance After the door has been open for 30 sec the temperature target 37 C and the COs content target 5 must have recovered to 98 of their set value after TI min The doors are checked for serviceability corrosion functionality and condition The door sealing are examined for wear seat and damage The serviceability and functionality of the hinges are tested The closing mechanism is checked for serviceability and functionality 6 The seal of the glass doors is checked The glass doors are examined for damage 7 The temperature controller MB is operated set values input and all keys checked for functionality The safety device 3 is tested for functionality set temp lower at safety device 27 8 EH The CO controller is operated set values input and all keys checked for functionality The CO cut off via the door contact is tested for functionality The main fan is checked for functionality The availability of the operating manual e g for safety information is checked The VDE test including determination of leakage current and insulation resistance is performed Ki for Germany other safety standards may be considered in other countries The temperature is re calibrated The CO is re calibrated Following service report should filled out during every maintenance and a copy should be handed over to the customer 48 CB 2 Service Manual state U 20U2 crea
15. not tightly Check the two door sealing Adjust the door keeping The door heating element is faulty Check the resistance normally 278 9 24 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 5 Most common service work Please note Please unplug when servicing or working on electronic part It s for your own protection 25 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger DI Changeing of the fan N If it is possible for the intended service work the mains plug should be pulled Note HA The fan motor turns when the chamber is RO switched on a Remove the rear wall of the chamber by use of a _ gt _ plus driver You have to cut the aluminium tape around the fan mounting plate Remove the two connectors to the fan motor Removed the 4 screws of the plate of the fan motor sheet metal frame 26 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger Unscrew the akis nut of the fan wheel left turning thread with a spanner oze i Remove fan Assemble the new fan motor in reversed order Replace Aluminium Tape 2 Paa er 4 Open the glass door and then the two screws at 4 7 the cover we Paa After unscrew you have to press down the cover gi to take it off Now you can change the steri filter The cover plate is fixed by 4 screws Before you can take ou
16. service purposes Description of CTMOT e The measuring system consists of 2 parts The measuring station which remains outside the CO incubator and the sensor housing which is put in the center of the usable volume of the incubator Both parts are connected by a ribbon cable which can be laid across the door gasket of the incubator without causing leakage e he measuring cell is placed directly in the atmosphere to be measured This means that there is no requirement for gas pumps whose inconstant flow rates can cause inaccuracies When converting the measured variable partial pressure CO to the display value VOL CO the respective altitude above sea level is entered and taken into account so that the display value always corresponds immediately to the real concentration in VOL COs irrespective of the place of use e he temperature is determined via ablO0 temperature sensor and is displayed on the large LED display in IO seconds alternation with the CO concentration e he measured data of temperature and CO concentration are output to an RS 422 interface Thus the measured data can be simple recorded stored and graphically represented via PC using the APT COM communication software developed by BINDER e For monitoring the nominal values set in the incubator can be entered into the mobile measuring system If these nominal values are exceeded or fallen below by permanently set tolerances this is reported by the unit
17. the Lemo socket might be unfixed Normal function no defect Sensor system is faulty The sensor head and may be the sensor board must be replaced The system must be re calibrated see chap re calibration The controller MB shows also a to low CO concentration Check the pressure of the CO bottle max bar min 0 bar oterilization process has to be off Check gas inlet valve When the door is closed the valve has to open with a clicking noise The door switch has to be in closed position when the door is closed If it is not closed no CO can flow inside the chamber Press the door switch by hand and check on the inlet tube whether CO is flowing in Check CO connection rear and the mm drill hole of the gas mixing head May be there is something jammed The controller MB doesn t show a to low CO concentration The CO Sensor System is defect The Sensor Outputsignal of the FPI Sensor board to the controller MB could be checked according the following chart Binder offers a calibration kit for this purpose with analyzed test gas with a CO5 concentration of 5 Vol Because the atmospheric pressure influences the measurement result of the CO sensor system the altitude of the site has to be taken into consideration Expected results during exposing the sensor head to 5 test gas HE Level Sensor current 8 00 mA 7 93 MA 200 m 7 86 mA 300 m 7 9 mA 400 m 7 72 mA 500 m 7 66 mA 600 m 7 60 mA 700 m 7555 mA 800 m 7
18. 3 79 6 Fi d 79 8 79 9 0 el 80 2 80 3 80 4 80 5 80 6 80 7 80 8 80 9 eil SN ei Su OTA OI OI OI OI8 OI0 82 82 82 2 82 3 82 4 82 5 82 6 82 7 82 8 82 9 83 SEN 83 2 83 3 83 4 83 5 83 6 83 7 83 8 83 9 84 84 84 2 84 3 84 4 84 5 84 6 84 7 84 8 84 9 5 ez 85 2 85 3 85 4 85 5 85 6 85 7 85 8 85 9 86 86 86 2 86 3 86 4 86 5 86 6 86 7 86 8 86 9 87 SI 87 2 87 3 87 4 87 5 87 6 SFT 87 8 87 9 88 88 88 2 88 3 88 4 88 5 88 6 88 7 88 8 88 9 89 89 89 2 89 3 89 4 89 5 89 6 89 7 89 8 89 9 0 OO 90 2 90 3 90 4 90 5 90 6 90 90 8 90 9 EN SEL OI HI 3 OTA Sa OI OI OI8 arg 92 Se 92 2 92 3 92 4 92 5 92 6 92 7 92 8 92 9 93 GEN 93 2 93 3 93 4 93 5 93 6 93 7 93 8 93 9 94 94 94 2 94 3 94 4 94 5 94 6 94 7 94 8 94 9 5 Su 95 2 95 3 95 4 95 9 95 6 95 7 95 8 95 9 96 96 96 2 96 3 96 4 96 5 96 6 96 7 96 8 96 9 97 95 97 2 97 3 97 4 97 5 97 6 gn 97 8 7 98 98 98 2 98 3 98 4 98 5 98 6 98 7 98 8 98 9 99 99 99 2 99 3 99 4 99 5 99 6 99 7 99 8 99 9 IO SALT 47 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 7 Maintenance The BINDER GmbH determines a annual service period to ensure the high quality and reproducibility of the results elaborated by help of this incubator Following tests and maintenance works must be
19. 49 mA 900 m 7 44 mA IODO0Um 7 39 MA this values are not reached with a tolerance of 0 24mA corresponds to 0 3 Vol Yo CO between the signal output of the FPI sensor board and the MB controller input from the sensor system is faulty The CO concentration doesn t go down after a door opening of approx 20s to 0 Vol CO The CO concentration drifts during exposing with 5 Testgas CO Controller shows 79000 There s also a red alarm bell blinking CO controller shows 9999 To low humidity inside the chamber Humidity to high inside the chamber Condensation inside Condensation at the glass door the table above during exposing with 5 COs Testgas If the deviation is within the tolerance of 0 24mA please make a re calibration If the value with the tolerance of 0 24mA at the signal output of the FPI Sensor board is reached but the controller doesn t show a value between 4 8 and 5 2 Vol CO there was a wrong calibration done Check the correct setting of the altitude and make a re calibration Make sure that you have reset the old calibration Check the altitude setting at the MB controller The Sensor system is defective or wrongley calibrated Make a re calibration and check if the value is now ok If not you have to change the sensor system Change first only the FPI sensor head and make a re calibration If the signal doesn t reach a stable value you have to change the sensor sy
20. ENJANG Sy paydnueguy si Su MRES SUM GMT Panjang Si dreoL SUID Jeg pue SIELUCINE ayp euojec ssas2ogd uongemttaie eui jo LOC Musa JE TIEN Zuu HOW H3CONIB Gi whudday JAQUES au apisia Atum au SEAU JO esHejoap peue to HH ST eques JEDA Dunesgnejapeu Jagweys au apisul Ajipiwnu ay jo jueugsn py aunpeooud siu Ag padonsap ag pe FOU au Auabawa raj pasn aq duo pinoys NPE eig peuede eg janu Sop eig JEU PIDAR 0 us petitingd uita at spa UO pug jo paying eq or Bey aur pooped eq ue ueresquajs AUB AWG aug mud e JENE A JE IB uo eaob out ATE Sup O SSL yg Din UD NEGUS au dors t SI HUN BINA S948 VOUS ALS LOU SUL Kar X j JUSLUUOIIALS o PJEZZEY jo 9589 ui Jaqweya Jauur au jo uonesimias s Ne JOH sus Bug BUO uo apigui paek ad ug yaa Jawan Sy jo jo aq oj su D AY Ope LCST JIE JOU IESEL Buoyed egag JeQweyo aug tJ PAU ag oj Sey Jens GO au STO ESBS TEN L HE L k HI ALAA byl FE o e Jequieua 3 00 BY JO Je JOH I sog us Pakua nG O Sau eas BU FAD HNS au SUE aus ay jo apnyye ayy jo Aug HEN ja NW One au sua e SUC NE Acum TEN IU
21. Jochen Tussinger 3 8 Flow Chart of the Permadry system basis CB wiring diagram Controller MB gt Permadry fan rz AL rota rong te ie 95 2604 A Analog Output 0020 od SE ES E Fan cold water basi n hiefoe Altii Plone 3 9 Description of the Function of the Fan Control The CB heats up to the engaged set value C just before the chamber reaches the set value the controller MB starts to pulse the heating signal The ON OFF rate is assigned by the regulation ration of the controller MI The higher the regulation ratio the higher is the fan speed If the chamber doesn t heat regulation ratio 0 the fan is working with his minimal speed If the chamber heats continuing regulation ratio I002G the fan is working with his maximum speed The speed of the fan is reduced to avoid kinetic influence of heating The rotation of the Permadry Fan is reverse proportional to the rotation of the Mainfan The only difference is The Mainfan is controlled constant with the help of a phase control level the Permadry Fan is in interval mode which is given by a solid state relay I CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 27I0 Sterilization Mode Depending on the resistance level of the germs attention must be paid to the quality of the sterilization procedure The lower the sterilization temperature the higher the risk of remaining germs and the longer the sterilization time is
22. MB controller shows not a reading between 4 8 or 5 2 Vol the MBT is not calibrated correctly or it is faulty Check the correct setting of the altitude above see level which is essentially for correct COs values displayed on MBl controller display see CB operating manual Try a re calibration see chapter re calibration with the correct altitude setting set before 22 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger MB controller display shows CO readings of I9to 20 Vol Yo CO also when there is no COs inside the chamber e g with open door FPI sensor board Red and green error LED flash alternately FPI sensor board Green LED flashes and lights alternately FPI sensor board Red LED is continuously on or flashes green remains off during normal operation CO too low inside the chamber par example cell medium is colored violet CO too low e his means the new plugged CO sensor head is not initialized successfully e Try a restart of the chamber by switching OFF and after JOsON an the main switch e If this worse reading still remains the sensor board has to be replaced and a re calibration must be carried out This means no connection between sensor head and the FPI sensor board The sensor head is not plugged correctly try to plug it again The red silicone wire between the Lemo socket for the sensor head and the FPI sensor board is faulty The soldered contacts of
23. O2 Vol U mV O2 Vol U mV 50 DI p 55 27 60 OIGA 65 935 4 70 I0 2 DO G IOA 55 WER GO SAERG D I 937 9 zu 073 7 50 2 SEA 55 2 ZIDA 60 2 820 9 65 2 940 5 70 2 I0 8 4 50 3 623 0 533 774 60 3 823 65 3 SEIN 70 3 IO8T 4 50 4 624 8 554 ZI0A 60 4 825 4 65 4 945 6 70 4 IOB4 4 50 5 626 5 59 5 724 4 60 5 827 6 65 5 948 2 70 5 IO8 4 50 6 628 4 55 6 723 4 60 6 829 9 65 6 950 8 70 6 IO000 4 50 7 630 2 oo 725 4 60 7 SEIN 65 7 953 4 70 7 UK 50 8 632 0 55 8 727 5 60 8 834 4 65 8 956 0 70 8 IO006G 8 50 9 633 8 55 9 729 5 60 9 836 7 65 9 958 6 0 9 OSS Di 635 6 56 37 Di 839 0 66 OGI3 IW I7I029 bi 637 4 D I 733 5 DI SEA Sch 963 8 T4 I706G0 DI 639 2 56 2 735 6 DI 843 6 66 2 966 5 2 3 I709 GAN 56 3 737 6 bli 845 9 66 3 969 Tic III23 DI A 642 9 56 4 739 6 DI A 848 2 66 4 O I8 ZA I77ID 3 BIS 644 7 56 5 Z4T SAS 850 5 66 5 974 4 I778A DI6 646 6 56 6 743 7 GIp 852 8 66 6 977 278 I7I278 Bi 648 4 56 7 745 8 DI Saa 66 7 979 8 8 I724 4 DI8 650 3 56 8 747 8 GI8 857 4 66 8 982 4 278 I72 0 DIO Esc 56 9 749 9 DI9 859 8 66 9 985 7 2 9 I7370 52 654 0 57 752 0 62 SS 67 987 8 72 I7342 D3 655 8 754 SEN 864 5 enr 990 5 72 I73 4 52 2 657 7 57 2 lc 62 2 866 8 67 2 993 2 72 2 I7A06 92 3 659 6 5 758 2 62 3 869 2 67 3 996 0 72 3 I74A38 52 4 D I A 57 4 760 3 62 4 SA 67 4 998 7 72 4 I74 0 52 5 663 3 57 5 762 4 62 5 873 9 67 5 IO0T A 72 5 0 52 6 665 2 57 6 764 5 62 6 876 3 67 6 IO04 7 72 6 I7b3b 52 7 D I 57 7 766 6 62 7
24. also the O Sensor Due to the sterilization units in copper version change their colour This does not have any negative effect on the function and quality of the unit Caution danger of burning Due to the increased temperature performances there is the risk of burnings at the marked unit parts and especially at the inner glass door Do not touch KG CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger AIZ Hot air sterilization with inner chamber contaminated with highly infective material If it is not possible to discharge the unit and to remove all necessary parts for safety reason the hot air sterilization can exceptionally be done nonetheless Attention Note that this procedure destroys the CO and the O sensor option Shut the outer and the inner door of the unit Switch on the unit Unlock the keypad of the controller MB with the key switch option unlocked horizontal position key is removable 4 Activate the emergency sterilization procedure in the CB Menu as following e re Enter Altitude Sterilization Emergency steriliz Humidity Normal User Code User Code Emergency Steri No TMP 487 5 CO 0 0 0 0 4 Display during the emergency sterilization 0 0 0 0 process AUTO _CONFIG Ww RESET MENUE _VIEW gt 20 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Caution Never o
25. ay controller MB for temperature and CO as well as O option B Connection socket for low tension supply option C CO sensor D Gas mixing head E PT IO0 temperature probe F 02 sensor option G Internal socket 230V max 3 A option H Shelf holder bar Shelves J Shelf holder K Lower housing cover L Permadry water basins M Inner glass door N Measuring access port CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 2 The Controller MBI Display Controller Mi Operating Light gt BINDER W 777 EXIT Button to leave a VA Menue ON OFF Button Stand by ENTER Button to confirm a selection Navigation buttons corresponding function will be assigned Mainswitch For the controller MBI it is possible to show all setpoints and actual values at the same time It is also possible to display the actual values portrary CB E2 Service Manual Na D U GA 5 Es O e d N O O A gt co O O D C D Na O state O I 2007 2 2 Short description of the MB Controller Souen Hawa NONIE o ubu WA Ua Eres IPB E 80 0994 86 FG C9 FL LO Bp n c l Hepta B8 4 Pes 29 ta 0 G 58075804985 Haws YIONIS Lic pC VOPR CL ELS is Uc PL GA HOLY Aa NOY i Uc a Gum ped pauso pute IAA Ob ZB LLY E9 pa D Bis BS III BUR jo sprs SO BLY IA O 26 LL LA pe 0 Gp lel pret mg 9 e A Eer a cr ctl aznap ay
26. ccording to the above table this corresponds to a voltage of 202 2 mV With ambient air the MB controller display should display 27 Vol According to the above table this corresponds to a voltage of 270 mV Setting Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct Analogue input 5 For Start Value X enter the voltage value that corresponds to the controller display eg 202 2 For Start Value W enter the reference voltage value of the O concentration of the ambient air 270 leave menu with 4 x EXIT Sensor with pushed on calibration cap unit door open unit in normal operation Sensor exposition to test gas 80 vol O for 5 minutes Comparison of table values With sensor exposition to test gas 80 Vol O2 the MB controller display shows e g 80 9 Vol 26 According to the above table this corresponds to a voltage of IA Dmy With sensor exposition to test gas 80 Vol O2 the MB controller display should display 80 Vol According to the above table this corresponds to a voltage of IA34my End value setting Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct Analogue input 5 For End Value X enter the voltage value that corresponds to the controller display e g 7475 For End Value W enter the reference voltage value of the O test gas 80 vol 7434 Set State to On gt leave
27. ddoy pad PALING Ou anjEA au Japus BAD Oy NONG ec lg Ol SUNG Wu ipa cudde you sue suo hen ddesoyo ft Bue 05700 uaBmoodAu so uabixcuad u Aq peouen jul q Ajeuomppe UES sheo aur jo uio eiui Jou 49095 ay up ipei US au jo uoggjodeAe Ag pasnes Ayuejauiso eq jo Bursearul prove oj episus Aypiunig 394 soy HBO SUOFeQnaul gD apisui ejeudsoune oz exe Guidaay Aq BIPSLU jas Brueulun2 jo swajs s sayng OSHEN Ag suongpuoo Hd peuysp yas oj SMOJE REGO SUL t ModE jo Sucpuos Japun spa EWWEW jo UGDA Jo paling g5 seules siojeqnau asn pauljapald bed sn jo abueg a aBugua oj Log up au pes c SUG cung syulod jas ou jo Anus Unies Leu ma rauen eur Ag usab sr uanoung ey unipeBiiagu Jog UOAIEISE B WUJUD Ou WORNT H3 LNA NO pue Ag purjs eed 650 OF uon pusod mesu e papa ol uoneuedo Jo ApEe dwe pfd LAN 3 0 0 088425 spun lonuog zoozeer Haug G 4 pum ur Anao LARA FSM apoo passe sn Dau Aun pagaen oe AE BUKANE jou Dua mau ay aeus a agnfug pasua au up ege Wun Biji pa amp ueus Du s mage BRN pius ea H ag o Sey nua ayj aGenfug Eu Bu wayo yo BEET uj u2uass4 pug ya bug tugunan ur paledan eg ura NUR po spear eu r Dia USAWA spe coup usus 5 MUN BUTLER Japun u usum Sinan out musa siu uium pasua aq we Bumasa Joer in 20r Dol Lo H
28. ervice Manual state U 20U2 created 03 2002 Jochen Tussinger 3 7 Function of the Permadry system Isotonic osmotic pressure ratios essential for the growth of cells are basically maintained in CO incubators by a maximum humidity content in the inner chamber During this process care must be taken to ensure the best possible protection against contamination The patented Permadry amp system guarantees a humidity performance of up to 98 relative humidity with completely dry inner walls The principle hereof is totally easy The double basin system consists of a large surface warm water basin an a cold water basin as defined condensation point The temperatures of both basins are in that way controlled that the humidification and the dehumidification are permanently balanced The Permadry amp system works completely free of disturbances or maintenance The handling of the Permadry system is the easiest thing and as safe as a coffee cup The basin is easily removable and can be refilled in or at the unit at any time Cold air produced by the Permadry fan streams through the air channel and cools the round cold water basin The condensation point is fixed by this N Eus S S The Permadry water basin must be placed correctly There is a description PRONT impressed EE Pl 5 Lettering FRONT gt Ta Gat is Ip CB 2 Service Manual state O 20072 created 03 2002
29. ibrations Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct Analogue input 5 Enter the value 0 to Start Value X Enter the value 0 to Start Value W Enter the value 7000 to End Value X Enter the value 7000 to End Value W Set State to Off leave menu with 4 x EXIT Determination and entry of the calibration values The incubator has to be pre heated at the working temperature common value 37 C for at least half an hour The altitude of the site above the sea level has to be entered to the temperature CO O2 controller The procedure is described in chapter 7 3 of the operation manual The chamber doors can be opened during the calibration procedure The original BINDER calibration cap is pushed on the O gt sensor tip only when exposed to the test gas not when exposed to ambient air The calibration is carried out in two individual steps At first the sensor is exposed to ambient air and afterwards it is exposed to analysed O test gas with known Os concentration Use the original BINDER one litre pressure gas bottle 0 bar The test gas bottle is connected via the original BINDER pressure reducer with flow meter and a plastic tube with the calibration cup The flow rate has to be situated between 600 and 900 ml min This is equal to the two highest graduations of the flow meter in upright position 4 Note The BINDER service uses a larger pressure ga
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31. l as the highest accuracy and selectivity The accuracy of the CO measuring system bases on a single beam infrared measuring cell which measures in differential mode with permanently alternating transmission characteristic of its semi conductor filter Due to this highly developed single beam principle with Fabry Perot interferometer FPI disturbance variables and aging phenomena in the measuring system are almost completely eliminated so that this measuring system in contrast to other measuring procedures remains practically drift free between calibrations and is absolutely selective for CO The CO measuring cell contains a measuring section inside in which the absorption of infrared light depends on the number of CO molecules in the beam path This number of CO molecules changes with the ambient pressure in relation to a constant volume The distances between the molecules are consequently pressure dependent The collision frequency of the IR beam with COs molecules increases therefore by increasing pressure For this reason the ambient pressure must be compensated in order to correct the display reading of the CO2 concentration in VOL This is achieved by entering the altitude of the site above the sea which is described in this manual 3 2 Function of the Heating System The temperature measurement is realized by ablO0 temperature probe which changes his resistance at different temperatures For example 37 C 774 380 see f
32. menu with 4 x EXIT The corrected value 80 vol 96 appears on the controller display after 5 seconds Afterwards the exposure with test gas is stopped The analysis result of the test gas acc to the label of the gas bottle is reached 43 AN 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 U ial US pus IA Lo Us la E s 2 2 2 2 2 3 2 4 See 2 6 d 2 8 29 3 A 3 2 3 4 3 5 3 6 3 7 3 8 3 9 4 di 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 5 5 2 5 3 5 4 5 5 5 6 uu 5 8 a9 6 Di 6 2 6 3 6 4 6 5 6 6 6 7 6 8 6 9 7 ZA 7 2 7 3 7 4 7 9 7 6 7 7 7 8 ra 8 ch 8 2 8 3 8 4 8 5 8 6 8 7 8 8 8 9 9 SC 9 2 9 3 9 4 Es 9 6 9 7 9 8 9 9 AN AR IO I0A I05 I0p IO IOUP MOS ITA I 3 E IIA iS I 8 e I 8 NK I3 IS ER E I2A ER I28 ta I28 2 8 E EN ER I33 I2A I35 I2p I3 KEE EK IA IAi E IA3 IAA IA IA8 IA IA8 IA2 eh ER ID IA d Ip T9 EE E Ip Ip Ip2 Ip IpG4A Ip IpGp Ip Ip KR t7 IZA K I A wes I p ke K Fe US EN I8 ER I84A ER ER to ER EK I0 kal ER 459 3 KEE dE ER ISA KEE 9 0 20 20 2 20 3 20 4 20 5 20 6 20 7 20 8 20 9 EA Se EA SlA ZIA Ska EAR 2l EAR SKS 22 22 22 2 22 3 22 4 22 5 22 6 22 7 22 8 22 9 23 SZ 23 2 23 3 23 4 23 5 23 6 23 23 8 23 9 24 24 24 2 24 3 24 4 24 5 24 6 24 7 24 8 24 9 44 5 Ac 25
33. mperature probe for inner temperature and safety device Inner view left double temperature probe for inner temperature and safety device middle gas mixing head socket of the FPI sensor head 30 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 5 4 Setting of the door heating This function is needed to stop condensation at the glass door Make sure that the outer and inner door seal tightly SW Offset Tur 4 020 Press the Button W up to SW Offset door press ENTER to confirm Now you can set the desired value Then press EXIT to come back to standard display The System Code is only for Service Engineers Don t give the System Code to the customer it is possibl eto change also the parameters so that the chamber couldn t work 3i CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 5 5 CQO Reference Measurement The COz measuring procedure of the incubator series CB is characterised by fast reaction times as well as the highest accuracy and selectivity The accuracy of the CO measuring system bases on a single beam infrared measuring cell which measures in differential mode with permanently alternating transmission characteristic of its semi conductor filter Due to this highly developed single beam principle with Fabry Perot interferometer FPI disturbance variables and aging phenomena in the measuring system are almost completely elimina
34. n of the COs concentration Vol 96 CO2 I8 Trade names of common NV media US 4 NaHCO3 g l 3_ DMEM 3 70 BME 2 20 MEM 2 20 Medium I00 2 20 NM Naa NE No mie 22 peg Y RA 6 8 7 6 pH value Example If a pH of 7 2 is measured in a medium which is buffered with 2 20 g NaHC03 per liter there must be 8 Vol CO in the surrounding of this medium 33 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 5 5 2 Measuring of CO directly via chemical indicator tubes This is a common do it yourself test of the users A chemical color reaction in a glass tube shows the CO concentration A standardized volume of air from the inside of the incubator has to be sucked through this glass tube to get a quantitative test result Therefor a special hand pump must be used with a standardized suction volume Example for such a procedure Il Break off both ends of such a glass tube or remove the plugs 2 Pin that end with the higher end of the scale on the adapter of the hand pump which belongs to that test system 3 Pin the other end trough the silicon plugged access port of the inner chamber door 4 of the CB incubator 4 Take one sample volume out of the inner camber volume by pressing the pump entirely together and releasing it afterwards 5 The standardized volume is sucked trough the glass tube and the chemical indicat
35. ng reference instrument 37 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Measuring uncertainty of the reference instrument C Calibration adjustment of the temperature controller KA NM necessary In case of No chap 3 can be skipped Notes Repeated calibrations are recommended in periods of I2 months Calibration alignment of the temperature controller Overview First of all the former calibration is reset see chap reset of the calibration The input value correction is deactivated afterwards set the state of the input val correction to Off Then return to the normal operation mode and let adjust the incubator for hour to the calibration temperature The display reading values of the inner chamber temperature Analogue input 7 of the preheating chamber temperature Analogue input 2 and of the safety controller are set to the display reading value of the reference instrument This is done by entering the display value of the reference instrument to the menu Input value correction of analogue input analogue input 2 and of the safety controller Finally the input value correction is activated set the state of the input val correction to On Reset of former calibrations Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct Analogue input 7 Enter the value 0 to Start Value X Enter the value 0 to Start Value W
36. older and the shelves are in the inner chamber Switch on the unit Unlock the keypad of the controller MB with the key switch option unlocked horizontal position key is removable 9 Activate the sterilization procedure in the CB Menu as following w SN ID AB I8 CB 2 Service Manual state U 20U2 created 03 2002 Jochen Tussinger Enter Altitude Sterilization Emergency steriliz Humidity Normal CO2 Probe outside CO2 P oba dulsice 7 NO STERI SEC3 00 00 05 08 44 05 O4TI2 0 TMP L 5 C02 0 0 0 0 O 0 0 0 O 96 Display during the sterilization process AUTO CONFIG W RESET MENUE VIEW Caution Never open the two unit doors during the sterilization process as the temperature reaction time will be interrupted and the sterilization will become ineffective IO The hot air sterilization is automatically finished after 3 hours Il Switch off the unit and open the outer door The temperature of the glass door handle is about 0 Use gloves or a tool e g pincers for opening the glass door or let the unit cool down as follows with opened front door for at least TI hour with closed front door at least 4 hours Please make sure that the unit has cooled down to ambient temperature but down to at least 60 C before plugging in the CO sensor In case of doubt please wait at least 2 hours I2 Befor putting into operation please plug in the CO sensor Optional
37. ollowing chart The CB is equipped with a double PTI00 one part is for the measurement inside the chamber the other part is connected to the safety device class 37 The measured value is evaluated in the controller MB The controller MB compares the set value and the now measured value and decides to give a signal current to the solid state relay 4K3 to activate the heating E CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 3 3 Flow Chart of the heating function basis CB 450 wiring diagram Pt UN FohLer in Imenroum 224 BIL i RegelfUhler 264 E T j NZ 7 W3 d amp 2 p3 Tt po B 3 a Controller MBI gt Gl 4 Bi GOA SV DC pi I G 0540 22 Connection Display The biO0 temperature probe is equipped with 3 cables two red and one white cable The white cable is connected to pin at El Ptl00 the two red cables at pin s 2 and 3 at El ROO0 To measure the resistance disconnect all three cables from the controller an measure between the white cable and one of the red cables do not measure between both red cables po fi Measuring of the HO resistance between the white cable PIN and one of the two red cables PIN 2 or 3 UK RS NN 0770 EM 000 53 Cao KS EN For Example Your resistance measurement system shows you I7I4A 280 Q this corresponds to 37 C 0 0 3 4 Controller MB PIN descripti
38. on Input Output BEI BEZ 853 BEA Ground TE Output Phase controll EI RO 3A IIb 220V AC Heater air duct BA2 7A IIP 220V AC E2 Rn O0 o Door Heater BA3 7A 7IIP 2320V AC pra E3 RO SC Collection Alarm BAI 2A 7I7IB 2320VN AC wa E4 4 20mA O Heater Kettle D BA4 60mA 5V DC E2 PO CO Solenoid valve BA5 60mA 5 DC E Safety device CI3 BAG 60mA 5 DC Wes Fan Cold water bassin E Safety device class 2 0 2 0 0 4 20mA Volatge output 45mA 24VDC Display connection 5422 E ee ECH 055 5 O Output Side CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 3 5 Function of the CO System The CO System measures the CO concentration inside the chamber This information is supplied by the FPI Sensor Head The Controller MB compares the engaged value with the now measured value and decides to open the solenoid valve of the CO gas inlet 3 6 Flow Chart of the CO System basis CB wiring diagram FPI Sensor gt FPI Sensor Board gt Controller MB gt Solenoid Valve gt Gas Injection Nozzle white 2 grey 3 green 4 yellow 5 blue 6 red 7 brown 8 pink 4 20m To Al A2 To Power supply at the Transformer Be ok Hi 45 264 A MBI Controller MBI Fh ti E4 4 20mA L DC H BAS 60m BV DC fr Solenoid Valve CO I3 CB 2 S
39. on with inner chamber contaminated with highly infective material 20 4A TOUDE NOON AA AA a 22 5 Most common service work 25 os ee E e eet EE 26 5 2 Take out of the electronic component board eene 28 5 3 Opening of the rear service lid to achieve the area of the air jacket 29 94 SEUNG Ol Me door NGANG MTN agan EN basada ance Pap ANAN 3i 5 5 COs Reference Measurement 32 Di Measuring of CO indirectly via the pH of the cell medium 33 5 5 2 Measuring of CO directly via chemical indicator tubes 34 5 5 3 Measuring of CO directly via a electronic measuring 080 0 6 35 C oie MNS TUS mm 36 SN R tselen e 0 ION EE 36 6 2 References for 0 0 0 nennen sese sese 36 6 3 Tolerance of Ie AdUSIMENL mo TOTEM 36 Calibration instructions for CO2 incubator CB with screen controller MBI 37 Temperature GO2 kaagungan E Nga es a a di Gu LAONG PAGING Telnet Gar aua aa nnn qa UG Mar kapanah 37 TIME Me AAA 37 Calibration alignment of the temperature controller
40. optically and acoustically as well as via a potential free alarm contact The alarm function only becomes active if both measured values were previously within the tolerance limits Measuring in a BINDER incubator type CB 0 Measuring station door is normally closed during measurement Sensor housing O Ribbon cable 35 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 6 Calibration 6 Definition of calibration In this service manual the word calibration means to adjust the actual value readings of the MI controller for the two controlled values of the CB incubator which are CO concentration and temperature to accurate references of known value Other words e g alignment are also customary for the same procedure 6 2 References for calibration Reference for temperature E A electronic measuring and display device for temperature which is traceable to a acknowledged standards calibration institution g DKD PTB for Germany with valid calibration certificate The cable to the sensor probe must be thin to cause no leakage E when it is laid between via the door sealing i Reference for CO concentration Two test gases with analyzed concentration are used to which the FPI sensor head is exposed directly BINDER offers a calibration kit with all necessary gases and accessories The third bottle is only needed for option Oz control li KT
41. or changes its color beginning from the side pinned into the chamber in direction to the hand pump 6 The more CO is inside the chamber the vast the chemical reaction will cause a change of the color of the chemical reactor 7 The CO concentration can be read off by the scale directly printed on the glass tube or a delivered reference reading rule It is necessary to correct the result with the current ambient pressure The necessary Example for chemical indicator tubes formula is printed on the instruction sheet of such systems I i Pa G n SA L D DN K i ba ik Notes All the necessary equipment must be delivered by only one manufacturer and one defined test system This test systems are nor very accurate A typical accuracy is around IO of the full scale value Therefor this system should not be used for re calibrating the BINDER FPFsensor system Example for hand pump foreground or a electrical pump background 34 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 5 5 3 Measuring of CO directly via a electronic measuring device The easiest way to measure CO concentration are electronic sensor systems BINDER offers the portable measuring device model CTM 0 which were specially designed to measure CO concentration and temperature inside of COs incubators The CTM 0 can be used both for reference measurements in certified laboratories as well as for
42. pen the two unit doors during the emergency sterilization process as the temperature reaction time will be interrupted and the sterilization will become ineffective 5 The emergency hot air sterilization is automatically finished after 3 hours 6 The incubator is no longer ready for operation Contact the BINDER service for repair As CO2 Sensor and or 02 Sensordestroyed Due to the sterilization units in copper version change their colour This does not have any negative effect on the function and quality of the unit Caution danger of burning Due to the increased temperature performances there is the risk of burnings at the marked unit parts and especially at the inner glass door Do not touch EA CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 4 Trouble Shooting Safety hints Do never unplug the CO sensor head while the chamber is switched on This could result in sensor destruction or initiation problems Hint The tradename of the controller is MI High CO consumption e f when the CO is connected the max pressure of Th bar is exceeded this can lead to a defect in the pressure switch The pressure switch becomes leaky as a result and loses gas CO too high inside the The controller MB shows also a too high concentration of COs chamber e MB controller output which controlls the gas inlet valve is defect par example cell medium is There are continually 5V DC at controlle
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44. r output BAS colored yellow CO too high e The solenoid valvet is mechanically defect it doesn t close e The controller MB doesn t show a too high concentration of CO The FPI Sensor System is defect The Sensor outputsignal of the FPI Sensor board could be checked according the following chart Binder offers a calibration kit for his purpose with analyzed test gas with a COs concentration of 5 Vol Because the atmospheric pressure influences the measurement result of the CO sensor system the altitude of the site has to be taken into consideration Expected results during exposing the sensor head to 5 test gas Sea Level Sensor Current 0m 8 00 mA IO m 7 93 mA 200 m 7 86 mA 300 m 7 9 mA 400 m 7 72 MA 500 m 7 66 mA 600 m 7 60 mA 700 m 7555 mA 800 m 7 49 mA 900 m 7 44 mA IODO0Um 7 39 MA If this values are not reached with a tolerance of 0 24mA corresponds to 0 3 Vol Yo CO between the signal output of the FPI sensor board and the MI controller input from the sensor system is faulty e Replace the FPI sensor head and check the sensor current against the expected value again during exposing the sensor head to test gas If the deviation is now within the tolerance of 0 24mA you can proceed a re calibration at the MB controller as described in chap re calibration to achieve maximum accuracy e f this values are reached with a tolerance of 0 24mA at the signal output of the FPI sensor board and yet the
45. s bottle with analysed test gas and an other flow meter But the procedure remains as described After 5 minutes exposing of the sensor to ambient air or to the test gas the entrance signal of the O sensor is corrected according to the table below and is entered to the MB controller according to the following description Correcting table for O calibration alignment of controller display The connection between the oxygen concentration of the test gas or the ambient air and the expected entrance signal to the MB controller is presented in the table below O Vol U mv 79 290 9 79 4 TIA0 79 Ap Reference value Reference value for air or BINDER test gas 4 452 d gi 484 7 Ab IAG0 DI A IA0p If other test gases are used see table in appendix for according tension values The measuring value correcting in the following description is carried out according this table by entering the expected voltage value and the voltage value X that corresponds to the actual controller display 42 CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger Procedure of O calibration alignment of controller display Sensor without calibration cap unit door open unit in normal operation Exposition to ambient air Gi vol 02 for 5 minutes Comparison of table values With ambient air the MB controller display shows e g 20 3 Vol O2 A
46. stem first only the FPI sensor head after that you have to make a re calibration Means measurement value underrange The Sensor signal between the Sensor Board and the Sensorinput E4 at the controller MB is low under 4mA Check if the sensor head puts correctly Check the cable between the Sensor Board and the controller M I looking for a break or a faulty contact Check the cable between the Sensor Board and the sensor If the controller gets the correct signal but shows still 20900 the input at the controller is defect The controller must be changed There s no current from the transformer 24VDC Check the power supply for the FPI sensor Possibly the fuse is blown T500mA Means measurement value exceeding The sensor signal between the FPI sensor board and the sensor input at the controller MB at Pin E4 is to high over 20mA or there s a initialization error try a restart If the red LED of the sensor board is lit permanent you have to change the sensor board If not check the sensor current and the sensor output of the sensor board Try a re calibration with analyzed testgas If the error reshows you have to change the controller The stpoint of the door heating is to high This is a internal setting of the controller Please see description in chapter 4 4 The Permadry fan doesn t run This fan is for blowing cold air throgh the air chanel to the cold water basin Check the outer door and the inner door fits
47. t the complete component board please check that the power supply is disconnect Please remove the drawn part of the air chanel of the Permadry system before you take out the component board Now lift the component board and take them out forwards Now it is possible to change all electronic parts If you plug in the power supply to measure some parts be carefull there could be 230V AC 28 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 5 3 Opening of the rear service lid to achieve the area of the air jacket This is necessary for following service works To replace the temperature probe of the air jacket To replace the socket of the FPI sensor head To replace the gas mixing head To replace the double temperature probe for inner temperature and safety device 9 8 If it is possible for the intended service work the mains plug should be pulled out Note The fan motor turns when the chamber is switched on Remove the rear wall of the chamber by use of a plus driver Cut the aluminum foil with a sharp knife at the upper area as shown Note that it should not be cut up all around Cut only the top line and the two sides 29 Fold up the insulation as shown beside Remove the parker screws which fix the metal cover Remove the metal cover sheet Now you have access to following paris e socket of the FPI sensor head gas mixing head double te
48. ted 03 2002 Jochen Tussinger C C Maintenance schedule CO Incubators series CB Type CB D n Working hour BEST CONDITIONS FOR YOUR SUCCESS Calibration Yes Service Station Contract Number UIT Working step Component ype Number recovery time CO and Temperature O MN general condition e door door sealing O O OJOJOO O rr 2 CO calibration Implementation features Maintenance issued by Date signature Steri filter NO MN O N NIN ISIS sch a lo O 0 OI gt ch co 0 gt OID COIN 0 gt ch NO N Customer confirmation Date signature Calibration certification handed over Date signature 49 Working step Working instruction Supplement Co general state optical test wear corrosion aaa LC E RR E O cc Sa 2222222 ZZ CU CH ru Cr o EH AO CCT Co CC adjust CO and Oo CO adjustment zero and one value 596 CO test gas O adjustment one measurement value 50 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger 8 Explosion Drawings of the CB E2 8 CB Component Board 2 4 IA 5 Number Description Ar cle No A4 Gas fine filter GO A 0006 4022 0077 CB 270 9 Mounting panel 4007 0074 A Pass clamp grey 5 Oo 6 8 H
49. ted so that this measuring system in contrast to other measuring procedures remains practically drift free between calibrations and is absolutely selective for CO The CO measuring cell contains a measuring section inside in which the absorption of infrared light depends on the number of CO molecules in the beam path This number of CO molecules changes with the ambient pressure in relation to a constant volume The distances between the molecules are consequently pressure dependent The collision frequency of the IR beam with COs molecules increases therefore by increasing pressure For this reason the ambient pressure must be compensated in order to correct the display reading ol the CO concentration in VOL 96 This is achieved by entering the altitude of the site above the sea which is described in the CB operating manual It is a common desire of customers to make test measurements between the re calibrations implemented as a part of the recommended annual maintenance work In principal there are 3 possibilities to test COs concentration inside a incubator which are described later on 32 CB 2 Service Manual state OTI 20072 created 03 2002 Jochen Tussinger DI Measuring of CO indirectly via the pH of the cell medium By use of the indirect determination of CO concentration via the pH value of the nutrient it is possible to check the CO concentration inside the chamber This method can not be used for re calibra
50. temperature Type CE LL LL of the measuring device Temperature calibration The temperature calibration is effected in one single procedure at the most common working temperature mostly used 37 C in thermal stationary condition The unit is checked in empty condition with one central shelf The reference sensor for temperature is led into the empty inner chamber through the door sealing and is positioned in the middle of the usable volume on one central shelf Both unit doors remain closed during the calibration The incubator has to be pre heated at the calibration temperature for at least one hour The adjustment of the temperature controller is only necessary in case there is a deviation between the temperature measured in centre of the useful volume and the reading of the actual temperature displayed on the controller MB or its safety controller of more than 0 2 K In case the reference instrument has a measuring uncertainty larger than 0 2 K see its calibration certificate this is the confidence criteria Test for the necessity of a temperature calibration adjustment of the controller Temperature set point W Display reading of the controller Display reading X of the safety controller in menu user settings Display reading of the reference instrument Divergence actual temperature Reading reference instrument Divergence actual temperature of the safety controller Readi
51. the display value of the controller e g 37 0 C to End Value X Enter the display value of the reference instrument e g 36 5 to End Value W Set State to On EXIT Inp Val correct Analogue input 2 Enter the display value of analogue input 2 e g 36 7 C to End Value X Enter the display value of the reference device e g 36 5 C to End Value W Set State to On EXIT Inp Val correct Safety controller Enter the value Safety control Act e g 36 9 C to Start Value X Enter the display value of the reference instrument e g 36 5 C to Start Value W Check and enter if needed the fix value 767 to End Value X Check and enter the fix value Abr to End Value W Set State to On leave the menu with 4 x EXIT The corrected value appears on the controller display after 5 seconds Result Calibration alignment of the temperature controller After the adjustment of the controller the temperature display reading of the reference instrument is compared with the display reading of the controller MB again Temperature set point of the Actual value shown on the test controller equipment CB 2 Service Manual state O 20072 created 03 2002 Jochen Tussinger 02 calibration alignment Reset of former calibrations Normal Display Config Configuration 2 5 System Code 2802 Service Level gt Inp Val correct gt Analogue input 4 Enter the value 0 to Start Value X
52. tion of the sensor system but it is a simple method to test for the correct CO concentration without any special CO measuring equipment Only a accurate pH indicator or a pH measuring electrode is necessary but this is standard equipment in cell culture laboratories This method bases on the acid base equilibrium of the buffer system in the nutrient The most common media are buffered by NaHCOs With the help of the pH value in the medium a conclusion can thus be drawn about the concentration of CO This conclusion is directly possible using the diagram below which expresses the interrelationship between CO concentration in vol and the pH of different NaHCOs buffered media We recommend the incubation of an empty sample with medium for II day under the same conditions as the cells The incubation can be performed in a cell culture bottle or in a 50 ml Falcon tube with open lid After gassing remove the empty sample from the incubator and within 5 minutes measure the o value with a glass electrode During the measurement the medium should have the least possible surface contact with the ambient air so that the CO can only diffuse out slightly Therefore transfusing should be avoided A significant downward movement will only be observed after 5 minutes allowing sufficient time for measurement Naturally pH test strips can also be used pH6 8 range not bleeding Following graph shows the pH value of NaHCO buffered media as functio

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