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Wellwash Versa User Manual

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1. fi Settings Wash head configuration Cancel The Wash head configuration window has the following parameters e Wash head You have to ensure that the installed wash head is the same as set in the Wash head configuration window The default is 2x8 96 Press OK to select the correct wash head from the list e I1x8 96 e 1x12 96 e I1x16 384 e 2x8 96 e 2x12 96 Warning An incorrect wash head configuration can cause liquid spillage A gt System The system parameters are set in the System window Go to the System row in the Settings menu and press the OK key fi settings Date and time 09 03 2010 11 09 52 Ci welw ye I ata aa Date format dd mm yyyy Time format 24 hour Buzzer Allon Language English The System window has the following parameters e Date and time The date and time is set in the dd mm yyyy hh mm ss format e Date format You can select the date format dd mm yyyy dd mm yyyy dd mm yyyy yyyy mm dd or mm dd yyyy The default is dd mm yyyy Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 71 Operating the Instrument settings menu Time format You can select the time format 12 hour or 24 hour The default is 24 hour Buzzer You can disable or enable the buzzer The default is Enabled Language You can set the preferred user interface language The language covers all parts of the software and the displayed language
2. Contact authorized technical service Use a different file name Insert a USB memory stick Table 11 10 Warning codes reported Code Explanation 101 WAI timer already timed out 112 Thermo Scientific Wellwash Versa User Manual Suggested action Thermo Fisher Scientific Troubleshooting Guide Troubleshooting guide Troubleshooti n The problems covered below are considered faults that require repair or p q p guide Symptom Dispensing volume is too low or residual volume is too high Dispensing volume is too low Volume is too low Wrong volume is dispensed Wrong volume is aspirated Plate is processed incorrectly Reagents are mixed Keys of the keypad do not function Display does not show all the information or anything at all Thermo Fisher Scientific Description Tubing is old or clogged Tips are blocked Filter is blocked The selector valve is not functioning properly Tips are deformed Leakage in tubing Dispensing valve is leaking when closed Wash head is incorrectly aligned Improper liquid calibration Underpressure in the bottle Too low maximum well volume has been defined Pump characteristics have changed Ambient temperature is too high or too low Overpressure in the bottle Pump characteristics have changed Wrong parameters are used A non active valve is leaking Keypad is broken Display is broken technical service immediately
3. Thermo Fisher Scientific gt D A Salt deposit Cleaning the plate carrier Thermo Fisher Scientific A Maintenance Cleaning the plate carrier Caution Although the Wellwash Versa is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from the outer surfaces to prevent damage and wipe them with deionized distilled water A Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods as damage may occur A 1 Turn the power OFF and unplug the instrument 2 Use disposable powder free gloves 3 Clean the instrument outside and the plate carrier with a soft cloth dampened with water or mild detergent 4 Ifyou have spilt infectious agents on the instrument decontaminate the instrument Refer to Decontamination procedure on page 102 Caution Do not use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish A Depending on the concentration of the wash buffers crystallization may occur around the dispense tips and container necks Therefore regular cleaning of these parts is essential Refer to Cleaning liquid containers on page 100 and Clean wash head on page 76 To
4. In addition this key can be used to terminate the possible computer remote control Thermo Fisher Scientific Lo abc Tal hee mno pqrs 637 Thermo Fisher Scientific def 3 tuv 8 ghi 4 WXYZ 9 10 11 12 Menus Main menu Info text bar Operating the Instrument Use the alphanumeric keys to enter numbers and text e The space character is found under the 1 key e The following special characters are found under the 11 key e The p character is found under the mno 6 key Use the CLEAR C 12 key to delete numbers or text Use the keys to select strips Refer to Strip selection with the number keys on page 41 To write a capital letter press the desired letter key repeatedly until the capital appears The internal software includes the Main Settings and Maintenance menus The menu layout is displayed in Table 5 3 Table 5 3 Program overview Main Settings Maintenance Protocol Prime amp rinse parameters Clean Head Plate L Sensors Calibration L Weall Wash head configuration Service Steps System Layout Reports You can specify the protocol related parameters and manage the run of the active protocol in the Main menu The Main menu is the one that opens when you start the instrument The Main menu contains the Protocol Head Plate Well Steps and Layout rows and their parameters f Settings j Protocol Head Plate
5. corrective work If problems orrur or reoccur contact authorized Suggested action Clean or replace the tubing Clean the tips Clean the inlet filters in the containers Component failure Contact authorized technical Service Change the wash head Clean or replace the tubing Component failure Contact authorized technical Service Check that the wash head is properly fitted Calibrate the wash head Do not cover the venting holes in the container caps Check the step parameter and adjust It Calibrate the dispensing pump speed Component failure or wear Contact authorized technical service Check the ambient temperature Do not cover the venting holes in the container caps Contact authorized technical service Check the parameters Component failure Contact authorized technical service Contact authorized technical service Contact authorized technical service Thermo Scientific Wellwash Versa User Manual 113 Troubleshooting Guide Troubleshooting guide Liquid is dispensed The plate orientation is incorrect Check the layout and place the plate in the correct onto the plate carrier orientation required by the wash head configuration The installed wash head is incorrect Check the wash head and replace the correct wash head required by the layout and the protocol Liquid is spilled during Well volume or shake speed Is too high Decrease the well volume or shake speed operation 114 Thermo Scient
6. e Pack the instrument according to the packing instructions e Use the original packaging for shipping e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department If the Wellwash Versa is exposed to potentially infectious chemical samples toxic or corrosive chemicals or radioactive chemicals waste Thermo Scientific Wellwash Versa User Manual 105 Maintenance Disposal of the instrument management of the complete instrument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to cc 2 Decontamination procedure on page 102 A Caution Observe all federal state and local environmental regulations A gt e Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Warning The used lithium Li battery is regulated waste and must be disposed of according to strict EPA Environmental Protection Agency requirements The Li battery has to be changed by an authorized service technician only Instruct
7. 11 and Figure 3 12 Note the difference in sizes e The larger thicker tube is the aspiration waste tube and is connected to the bottom hole e The smaller tube is the dispensing tube and is connected to the top hole 24 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Installation Installation setups e Apply silicone grease to the O rings of the tubes if necessary The small and large tube on the left side are blue for easier e recognition lli e y ll i Figure 3 11 Wash heads A and tubes B 2 Fit the wash heads next to each other onto the wash arm Figure 3 12 Figure 3 12 Fitting the 2 x 8 wash heads next to each other onto the wash arm 3 Check that the wash heads are evenly inserted Figure 3 13 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific 25 Installation Installation setups Figure 3 13 2 x 8 wash heads evenly inserted 4 Ensure that the wash heads are in the correct order Figure 3 14 5 If only one wash head 1 x 8 or 1 x 12 is used place the blank wash head onto the left of the wash arm Figure 3 14 Fitting the blank wash head onto the left of the wash arm 6 Enter the new wash head configuration in the Settings menu to correspond to the installed wash head The information is used to check for protocol wash head mismatches Refer to Wash head configuration on page 70 26 Thermo Scientific Wel
8. Lz v m T T T P Thermo Scientific Wellwash Versa User Manual Rev 1 0 Cat no N11166 Copyright Copyright 2010 Thermo Fisher Scientific Corporation All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks Nunc and Wellwash are registered trademarks of Thermo Fisher Scientific Inc and its subsidiaries All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots The version number displayed in screenshots may not always be the one of the currently released version Screenshots are only replaced if the content related to the application has changed No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Power failure The system requires un
9. Yell Flat Maintenance Untitled 1x6 96 The clock on the menu bar shows real time Thermo Scientific Wellwash Versa User Manual 47 Operating the Instrument Main menu See the info text bar for the required actions of the F1 F3 keys The action text on the info text bar changes according to the current menu Liquid level The info text bar also shows the liquid level in the containers A B R detection 22 W The liquid containers have sensors to enable constant and automatic liquid level detection during runs Fill the buffer containers and empty the waste container if the red bottle icon appears on the info text bar Refer also to Sensors on page 70 Liquid level detection LLD Liquids A B and Rinse e bottle icon is green when the container is full e bottle icon is red with an exclamation mark when the container is near empty e bottle icon is empty with a cross when the LLD is disabled Waste e bottle icon is red with an exclamation mark when the container is full e bottle icon is green when the container is not yet full e bottle icon is empty with a cross when the LLD is disabled Protocol The Protocol row in the Main menu shows the name of the active protocol An asterisk in front of the protocol name indicates unsaved changes to protocol parameters You can open another protocol by pressing the OK key on the Protocol row or by pressing the FILE key The list of saved protocols opens The
10. accuracy 2 83 85 86 98 active calibration 82 86 adding a new step 54 60 application 2 5 aspirate 35 55 60 62 63 98 108 mode 62 63 108 aspiration 13 24 35 58 62 63 66 108 height 58 62 63 108 time 62 63 autoprime 69 autorinse 69 buffer 27 28 29 34 37 38 40 48 61 64 66 70 77 78 79 93 107 container 27 37 48 source 27 61 64 66 buzzer 66 69 72 C calibration 47 73 82 83 85 86 87 113 curve 83 clean 22 30 47 73 74 76 97 98 99 100 103 113 wash head 74 76 78 99 cleaning 3 22 73 74 76 77 78 79 80 97 98 99 100 103 disinfection procedure 22 74 103 liquid containers 97 99 100 procedure 22 comment 66 connecting to a computer 32 D date and time 71 73 date format 71 decontaminating the instrument 74 93 97 99 102 103 106 121 Thermo Fisher Scientific decontamination 74 93 97 99 102 103 105 106 121 123 Certificate of 103 105 121 procedure 74 93 97 99 102 103 106 121 default plate 59 deleting a step 56 60 dispense 35 60 61 62 64 87 98 99 108 111 accuracy 108 height 62 64 speed 87 volume 64 108 dispensing 13 24 34 35 61 84 85 86 87 88 113 disposal of instrument 105 disposal of materials 100 101 E EIA 102 123 ELISA 13 123 empty priming vessel 74 81 82 environmental requirements 22 error 42 73 105 111 112 codes 111
11. on the instrument user interface Help errors and warnings log files and exported data The default is English NOTE Languages other than English are not yet available e English HX Chinese e Francais French e Deutsch German HASH Japanese e Portugu s Portuguese e Pycckuit Russian e Espanol Spanish The following information related to the instrument is also visible in the System window Version Version of the internal software SN Serial number of the instrument Protocol ver Version of the protocol parameter set The version can change with a new internal software version For the best compatibility ensure that the protocols are of the same version Memory used The amount of protocol memory that is in use as a percentage of total memory Reports You can export the available reports to a USB memory stick for viewing printing or saving them To export a report attach a USB memory stick to the USB memory stick port in the instrument select the desired report and press the OK key The selected report is exported to the USB memory stick under an automatically created folder named Wellwash 72 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Maintenance menu __ Settings 13 10 Status The reports available are e Error log The error log shows the date and time of the error and the error
12. protocols are listed in alphabetical order by protocol name The maximum number of available wash protocols is 99 48 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Main menu Protocol Modified Create new protocol Demot 2x8 96 04 03 2010 11 35 Demo2 04 03 2010 11 37 2x6 96 04 03 2010 11 38 Cancel i Itis possible to protect protocols from accidental editing or deleting A Creating a protocol Thermo Fisher Scientific locked symbol is shown in front of the protocol name when locked To lock a protocol select it on the protocol list and press the F3 Lock key For more details on opening a protocol refer to Selecting a protocol on page 38 For instructions on how to start create and save protocols refer to Chapter 4 Running Wash Protocols and Creating a protocol on page 49 Caution If the wash head format of the protocol that you want to open is different from the wash head format that is currently installed in the instrument all the protocol parameters are reset to their default values A Caution Change the wash head format to match the one used in the protocol before opening such a protocol to avoid resetting the protocol parameters A Untitled 1x6 96 1 Press the FILE key in the Main menu to create a new protocol Refer to Protocol on page 48 Thermo Scientific Wellwash Versa User Manual 49 Oper
13. 12 or 2 x 12 wash head is selected The strips are numbered 1 12 if an n x 8 wash head is used The strips are Thermo Fisher Scientific Thermo Fisher Scientific Operating the Instrument Main menu marked A H if an n x 12 wash head is used The strips are numbered 1 24 if a 1 x 16 wash head is used If there is no Layout step in the protocol the processed area is according to the Main gt Layout row selection Note Only the strips that are selected to the Layout step are saved with the protocol Strips selected using the Layout row or the number keys are not saved A Select unselect columns with OK key Move cursor with left or right arrow key Clear all by pressing C key Strips 1 12 are edited if an 8 way wash head is used or strips A H if a 12 way wash head is used The selected strips are highlighted with red color Select unselect columns with OK key Move cursor with left or right arrow key Clear all by pressing C key If the Layout step is the first step in the protocol the selected wells are shown in blue color on the Layout row in the Main menu The Layout row is also locked Thermo Scientific Wellwash Versa User Manual 67 Operating the Instrument settings menu Layout row Settings menu Prime amp rinse parameters 68 Thermo Scientific Wellwash Versa User Manual Protocol Demos Head Plate 1x8 96 1 Layout 2 Wash If the Layout step is not the first step i
14. 3 1 mm Max overall plate height 14 5 mm 0 2 mm 449824 Nunc U96 U 4 2 mm 14 5 mm 0 1 mm 430341 Nunc C96 C 3 4 mm 14 0 mm 0 3 mm 249662 Nunc V96 V 4 1 mm 14 5 mm 0 1 mm 441653 Nunc starwell strip plate Star 3 6 mm Footprint 9 0 mm Pitch size center to center 9 0 mm 384 wells 464718 Nunc F384 Flat 2 mm Max overall plate height 14 4 mm 0 25 mm Footprint 4 5 mm Pitch size center to center 4 5 mm Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 59 Operating the Instrument Main menu Steps Wash 60 Thermo Scientific Wellwash Versa User Manual You can create or edit the wash protocol in the Main menu Steps are listed and numbered in the order of execution It is possible to create several steps in the step list and process the steps in the defined sequence Steps can be used several times in the user defined order In the majority of applications the protocol has only a wash step A protocol can have a maximum of 99 steps If there is more than one step in the protocol the step parameters are not shown on the Steps row due to lack of space Refer to Adding new steps to protocols on page 54 and Deleting steps from protocols on page 56 The following steps are available e Wash e Aspirate e Dispense e Soak e Prime e Pause e Layout Liquid is aspirated and liquid is dispensed into the well Simultaneous dispensing and aspiration is possible with greater volume or lower
15. 40 Run a protocol for example one of the demo protocols available with the instrument The protocol should have an aspirate dispense shake and soak function to ensure an adequte sample of functions to test the instrument s proper operation Check that the liquid channels work properly during dispensing and aspiration To ensure that each channel works properly change the channel that is used in the dispense step and run the protocol again If you want to change for example the wash head s or the language modify the required settings in the Settings menu Refer to Settings menu on page 68 Thermo Scientific Wellwash Versa User Manual 35 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 36 uw Soom Ace Son 1 2 3 fA Thermo Fisher Scientific j 5 6 pars 7 tuv wxyz 0 c e5 9 100 17 012 Chapter 4 Running Wash Protocols To run a wash protocol l 10 Ensure that there is sufficient liquid in the used buffer containers A B or Rinse and that the Waste container is not full when you begin The liquid levels in the containers are automatically monitored during runs Follow the color coded bottle icons on the info text bar Refer to Filling and emptying liquid containers on page 38 Select a protocol Refer to Selecting a protocol on page 38 The selected protocol name is shown on the Protocol row in the Main menu Insert the 96 or 384 well plate
16. 89 Dapo aul etear a0 vo co o 6 keneeaneme emer Cre erate ne nee een tte eee te ee 90 Exportiie a protocol 45 text files c tees cae acdc eee 91 SMUL siss inna 93 Dt el AON e ee ed eel tee 93 Emergency Situations sccssssssssssseessssssssesseessseeseseseesnseesnsasseeaes 95 Handling emergency SiC AatiON So tiyacweatdapsndiets ante ineine 95 Maintenant E sscisccecdicstecusnsenenvchiavenasvtsiarsdvaiunasndvanetiecswsauigionnntiveevesstvaesusaiveses 97 Regular and preventative maintenance ssssesssesesesssesseressresseeeseres 97 Mantenance checklistirarenar erano na N NSA 97 lnstrument CATS Aerien inn E E A 98 Cleanins the Instinct E E 98 SEDO a a E eer ner ere nese oe erry ne eer 99 Ge Toul eyed oan plate Car ule quer ter ren eet eer ner nner Perret arnt ene rere 99 Cleanine ligtiid COntainers oses least aa Gain 100 Replacing the wash Read S jcdccccesncccetncazsheatanttendidenoeaneeaceascneeees 101 Disposal of matrial ennea a dake ian A E 101 Decontamibanon proceduto T e E 102 Retittiig the transport lOCK sersniscsrsnnin eneore aa 103 Maintaining a system log serenana amie 105 PACKING TOR SErVI CE soa csestvacnrsedadeeasesticxeacetaseneeet sarees Gonteniabeenteteses 105 Disposalof the instrument sne ees tiiiungteas eran 105 Technical Specifications ccssssssessessseseeesesesssseeeseseseeseanaess 107 Generals pecic Ons opne E 107 Performance specication esistia n E 108 Salty SPECICATION a nwa a E E A 109 In
17. Create new protocol ox 5 Select Export with the Down arrow key and press the OK key The protocol is exported to the USB memory stick under an automatically created folder named Wellwash The file extension of an exported protocol is PRO The file is in binary format You can export protocols one at a time Importing a protocol To import a protocol from a USB memory stick to the instrument 1 Insert the USB memory stick Figure 6 25 2 Go to the Protocol row in the Main menu and press the OK key The list of protocols appears Protocol Yell Modified Create new protocol OK 3 Press the FILE key to open the File menu 90 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Exporting and Importing Exporting a protocol as text file Create new protocol 4 Select Import with the Down arrow key and press the OK key A list of protocols stored on the USB memory stick opens Well Modified 1x6 96 Flat 10 02 2010 10 57 1x12 96 Flat 04 02 2010 15 21 1x16 384 Flat 04 02 2010 15 47 5 Select the protocol you want to import with the Down arrow key and press the OK key You can import protocols one at a time The protocol is imported to the instrument and it is available on the list of protocols It is possible to import protocols also from the Wellwash instrument Exporting dq You can export a protocol as a text file to a USB memory stick The t
18. Refer to Prime amp rinse parameters on page 68 The layout editing of the plate or strips can be carried out in two ways either by using the number keys or the Layout row in the Main menu It is possible to select the strips to be processed on the plate with the number keys Settings Maintenance Protocol Untitled Head Plate 1x6 96 Well Demol Demo3 The following rules apply when the number keys are used e 1x8 wash head strips 1 to 12 any combination e 1x 12 wash head strips A to H any combination press number keys 1 8 respectively e 1x16 wash head strips 1 to 24 any combination press number keys 1 12 to select two strips at a time respectively e 2x8 wash head any combination of two adjacent strips Only an even number of strips can be selected e 2 x 12 wash head any combination of two adjacent strips Only an even number of strips can be selected e Press the corresponding number key to select a strip e Press the corresponding number key to unselect the strip e Double press a key to select all unselected strips from this key down to a selected strip if that exists An even number of strips are selected with 2 x wash heads e Double press a key corresponding to a selected strip to delete all selected strips in descending order An even number of strips are deleted with 2 x wash heads All selections are deletable Thermo Scientific Wellwash Versa User Manual 41 Running Wash Prot
19. conformity with the requirements sessesessessssessessrrssseresse 109 Troubleshooting Guide ccccssssssssseesssesessesessesseseesesesesseseesneersneas 111 FO UN Wy ATENEO CS asper eatin tee E tecactiens 111 Troubleshootine SUIS srsnereraierai a a eres 113 Ordering Informatio ianasscsansucisannndaaan 115 W ellwas h V eisie E E 115 List Of Sate parts and accessorie ienai n N T terstinteeveaees 115 histor Thermo Sciemtinie platescesniicnireitnn n 116 System L rien 117 Thermo Scientific Wellwash Versa User Manual 9 Contents Appendix B 10 Thermo Scientific Wellwash Versa User Manual Certificate of Decontamination Thermo Fisher Scientific Contents Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 11 Contents 12 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Intended use Thermo Fisher Scientific Chapter 1 Introduction to the Wellwash Versa Microplate Washer The Wellwash Versa Figure 1 1 is a microplate washer It is designed to use both 96 and 384 well plate formats the 96 well plate in both landscape and portrait orientation and the 384 well plate in landscape orientation The 96 well plates and strips are designed to be washed in l x 8 2 x 8 1 x 12 or 2 x 12 format and the 384 well plates in 1 x 16 format The instrument also allows shaking during soaking The instrument has an intuitive graphical user interface In addition the Wellwash Versa
20. default is A Dispense volume pl For a 96 well plate the dispense volume range is 50 pl to 400 ul in 10 pl increments and the default value 300 yl For a 384 well plate the wash volume range is 20 pl to 120 ul in 10 ul increments and the default value 100 pl Note Because aspiration is on during dispensing the residual volume in the wells depends on the dispensing height A Dispense height start The dispense height start ranges from 0 mm to 20 mm in 0 1 mm increments The default is 17 0 mm Ensure that the value fits the plate in use Because dispensing starts from a lower level in a well and ends at a higher level the start height should be lower than or equal to the end height Dispense height end The dispense height end ranges from 0 mm to 20 mm in 0 1 mm increments The default is 17 0 mm Strip over mode You can define the strip over function The default is No e No All selected strips are processed as fast as possible e Yes If Yes is selected unused strips are processed dry The time parameters are measured during the first protocol run The wash head stays above unused strips for as long as it takes to process each selected strip Thermo Fisher Scientific Thermo Fisher Scientific Prime Operating the Instrument Main menu During a soak step the liquid remains in the wells for the set time with or without shaking of the plate or strips The soak step can be used in a protocol
21. shape has an effect on the aspiration height and aspiration mode A Flat U V Star A Star B Flat Flat bottom Round V bottom Flat bottom Showing the bottom with curved bottom with curved orientation edges edges and 8 ribs of the 8 ribs 58 Thermo Scientific Wellwash Versa User Manual Figure 5 24 Well shapes Thermo Fisher Scientific Operating the Instrument Main menu e Well offset Adjust the X offset of a plate Adjustment is required primarily with a 384 well plate The offset value ranges from 1 5 mm to 1 5 mm in increments of 0 1 mm Caution With 384 well plates check that the offset limit is not too big to prevent dispensing outside the wells A Use the up and down arrow keys to adjust the value Press the F3 Preview key to move the plate carrier and align the wash head over the first column of a plate Then press the left or right arrow key to move the plate carrier in steps of 0 1 mm Caution Well offset has an effect on the residual volume in the well A Press OK to accept the value and or F2 to close the window Microplate requirements Both 96 and 384 well format microplates can be used with the Wellwash Versa Table 5 4 dimensions A Note Use only plates manufactured according to ANSI SBS standard Table 5 4 Microplate requirements Well Default Default plate Bottom Bottom Dimension name Plate dimensions plate plate code designation shape height 96 wells 439454 Nunc solid F96 Flat
22. the caps of all containers to avoid overflow of the waste container and to warn the user when the liquid containers are almost empty The different reagent sensors and the waste sensor can be enabled or disabled in the Sensors window OK Go to the Sensors row in the Settings menu and press the OK key Settings sensors Buffer B sensor Enabled Buffer Rinse sensor Enabled Buffer Waste sensor Enabled Plate sensor Enabled The Sensors window has the following parameters e Buffer A sensor You can disable or enable the buffer A sensor The default is Enabled e Buffer B sensor You can disable or enable the buffer B sensor The default is Enabled e Buffer Rinse sensor You can disable or enable the buffer Rinse sensor The default is Enabled e Buffer Waste sensor You can disable or enable the buffer Waste sensor The default is Enabled e Plate sensor You can disable or enable the plate sensor The default is Enabled When enabled the instrument checks whether there is a plate on the plate carrier at the beginning of the protocol run Wash head You must always set the wash head configuration to correspond to the configuration physically installed wash head Refer to Head Plate on page 57 Go to the Wash head configuration row in the Settings menu and press the OK key 70 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument settings menu
23. the instrument g capabilities specifications Table 10 8 Performance specifications Performance specifications Wash volume 50 1000 ul in 50 ul increments 96 well plate 20 120 ul in 10 ul increments 384 well plate Wash cycles 1 10 Residual volume Aspiration height Dispense volume Dispense accuracy Dispense precision Soak time Rinse time Rinse volume Prime volume Memory Linear shaker 108 Thermo Scientific Wellwash Versa User Manual lt 1 5 ul per well with sweep aspirate mode and with high aspirate speed at room temperature flat bottom 96 well plate Adjustable 0 14 mm in 0 1 mm increments 50 400 ul in 50 ul increments 96 well plate 20 120 ul in 10 ul increments 384 well plate 10 300 ul at room temperature with 2 x 8 wash head CV 3 300 ul 96 well plate 1 s 60 min in increments of 1 s min 1 150 s in increments of 1 s 5 100 ml in 5 ml increments 5 100 ml in 5 ml increments 99 protocols Amplitude 2 5 mm 5 Hz Amplitude 1 5 mm 10 Hz Amplitude 1 mm 15 Hz Thermo Fisher Scientific Thermo Fisher Scientific Safety specifications In conformity with the requirements Technical Specifications Safety specifications This section describes the safety specifications for the Wellwash Versa instrument Wellwash Versa bears the following markings Type 888 100 240 Vac 50 60 Hz 100 VA max Class CE mark Wellwash Versa conforms to the following requireme
24. to be washed onto the plate carrier and ensure that the microplate is correctly oriented according to the wash head configuration The A1 position of the plate is in the upper left corner when the n x 8 or 1 x 16 wash head is in use and in the lower left corner when the n x 12 wash head is in use Refer to Loading the plate on page 39 Ensure that the plate is not covered Press the PRIME key to prime the instrument Refer to Priming the system on page 40 Select the strips if they are not already selected Refer to Strip selection on page 41 Press the START key The microplate is washed automatically according to the predefined protocol If you want to abort the run press the STOP key during the run Remove the plate after the wash protocol Rinse the instrument Refer to Rinsing the liquid system on page g q y pag 43 Thermo Scientific Wellwash Versa User Manual 37 Running Wash Protocols Filling and emptying liquid containers Filling and emptying liquid containers Selecting a protocol Selecting a ready made protocol 38 Thermo Scientific Wellwash Versa User Manual To fill or empty the liquid containers Remove the liquid containers and fill or empty them when necessary Refer to Liquid level detection on page 48 2 Unscrew the liquid container cap and fill with a suitable buffer solution 3 Replace the liquid container cap and reinstall the liquid or waste containe
25. together with other steps soak parameters soak time mm ss shake speed The Soak step has the following parameters e Soak time mm ss The soak time is the time period between a wash cycle in microplate wash protocols The minimum value is 00 00 s and the maximum value 60 00 min in increments of 1 s The default value is 00 01 e Shake speed You can define the shake speed Off Low Medium or High The default is OH The shake speed setting is effective only when the soak time is gt 00 00 Caution Ensure that the liquid does not spill at the shake speed and volume settings that you use A Priming is performed to fill the liquid system of the instrument with liquid and to remove all air from the tubes A priming step must also be performed when switching between buffers A protocol should have prime steps for the proper channels to ensure that the tubing is filled correctly with the desired liquid before performing any wash or dispense steps The tubing volume should be at least 15 ml to fill the line all the way from the intake port to the wash head A prime function is requested before a protocol is started and it can be accepted or rejected The prime step can be used in a protocol together with other steps If the instrument will be left to stand for a longer time priming must be performed with air to remove all liquid from the system Raise the tube from the liquid to allow air to enter the tube Note Avo
26. 02 To clean or disinfect the instrument 1 Press the OK key on the Cleaning Disinfection procedure row Maintenance 2 Empty all containers and fill the rinse container with cleaning reagent and connect all tubes to the rinse container Press the OK Maintenance Cleaning Disiry ti a oe ga ai Move wash he OK continue F2 cancel 3 Select the soak time using the Right arrow key and number keys ox The default is 15 min Press the OK key Priming preparing the soak and soaking take place 74 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Thermo Fisher Scientific Operating the Instrument Maintenance menu Maintenance Cleaning Disinfection procedure Clean wash hi Soaking 00 14 53 soak wash he Move wash head Empty priming vessel Continue Press the F2 key to abort the soaking if you want a shorter soak time than the set soak time and to continue the procedure Priming is then carried out Maintenance Empty all bottles Fill rinse bottle with distilled water Empty all containers and fill the rinse container with distilled water Press the OK key Priming preparing the soak and soaking take place The default soak time is 2 s Finally the priming vessel is emptied Maintenance Cleaning Disinfection procedure Clean wash he Emptying priming vessel Soak wash he Move wash head Empty priming vessel Thermo Scienti
27. 20 ml in 1 ml increments or it can be set OFF The default is 5 ml e Rinse volume Rinse button ml The rinse volume ranges from 5 ml to 100 ml in 1 ml increments The default is 30 ml e Autorinse The washer can be programmed to rinse the system automatically using the rinse liquid with a selected volume At the end of the run the software starts to count down the selected time 30 seconds before the autorinse starts the buzzer gives an audible sound and the remaining time is shown on the display When the time reaches zero rinsing starts e Volume ml The autorinse volume ranges from 5 ml to 100 ml in 1 ml increments The default is 30 ml e Time hh mm The times range is from 00 00 Off to 12 00 in increments of 1 h or 1 min The default is Off e Autoprime Periodically primes the instrument at a set interval to ensure that the instrument stays primed and ready to be used at all times Autoprime is not recommended for long time intervals overnight instead use the soak wash head function Refer to Soak wash head on page 78 e Volume ml The autoprime volume ranges from 1 pl to 30 ul in 1 pl increments The default is 5 pl e Time hh mm The time ranges from 0 00 Off to 9 00 in increments of 15 min The default is Of Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 69 Operating the Instrument settings menu Sensors Liquid level sensors are built into
28. 300 pl The tare weighed mass of each plate is entered in the display Whenever a mass is entered the mean volume per well and accuracy is calculated All wash head configurations have their own calibration curves done at the factory Calibration is only done to the active wash head defined in the Settings menu The calibration is performed with the original factory values To carry out the gravimetric calibration Select the Calibrate current wash head row using the Down arrow key and press the OK key Maintenance Calibration Pump calil Active cal jai Default Calibrate Starting calibration of 1x8 96 wash head OK 2 Press the OK key to start the calibration of the wash head Thermo Scientific Wellwash Versa User Manual 83 Operating the Instrument Maintenance menu Te Maintenance j Calibrate current wash head Weight Yolume Accuracy 3 Select the used channel using the Up arrow key and or press the OK OK key j Maintenance Calibrate current wash head Weight Yolume Accuracy 4 Insert the first tare weighed plate onto the plate carrier and press the F1 key to continue Dispensing is carried out i Maintenance Calibrate current wash head Weight 5 Weigh the dispensed plate and enter the mass of the dispensed OK liquid using the number keys Press the OK key when ready to 84 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scie
29. 4 SO aE ETE steele oie vena fo eeapsoena 65 gi bss omer eae er ee nae eee eer a ee ner ee ee a geen ee 65 PAIS ates E E ia ear E T 66 Ay OUI E tint Gay E E E A Seanean tian A icine ausaencnieun 66 TeAVOUE iO Wanni r a N 68 SREI a A e E E A T E E ET 68 Pimec Tinse pardinete Siasea a pee aed 68 DEOS a EE N E AEE 70 Wash head contier oim ancsi n a e 70 DSCC a AE E A even AE E E ET es 71 PS DOE 6 saccade ue cose sa sce ae N EAA ELAI A AAAA N TETEA Le INVALINECN ANCE SINC 1 aise hasan steam A 19 OS a EE E E A A AAE E tay eae 73 Cleaning Disinfection procedure sseseseseesseseeseresesesseressressees 74 Cran 95 hhee e TAa 76 SOak Wash Nead ic xyaessciasaerdioveboted oan pecan 78 Move Was ly TCA eae tce scoala tats ee cleo a 80 Empi primine Vesse breina a a 81 CAUDAN Co 5 Renee erator ene etre ene ee en Seer nna nner 82 PNG CIVG CANUT ON Aes n ca canine a NEA 82 8 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Appendix A Contents Calibrate Current wash CAG ici de ccassvcccsinechitvseeaunseertadiecewaneetentss 83 DEV ICS E E E E E EE ceed cee ae ae 87 Calibrate dispensing pump speed eee seeeeseeeeeseeeeneeeeeneeeeeaees 87 Exporting and Importing ccssscssssssssesssseecesseeseseseeeeeseeeeeeiseeeens 89 EXPOLtIN a PLOLOCOl sos s ccbserwiaiecersaeresd aden n a aos
30. 7 27 37 38 40 48 60 65 89 volume 61 64 108 washing 13 61 waste 4 24 27 28 29 30 31 34 37 38 48 70 93 100 101 102 105 106 109 117 container 27 30 31 34 37 38 48 70 93 100 101 102 well shape 58 62 63 Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 127 Notes 128 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 129 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 PO Box 100 Fl 01621 Vantaa Finland www thermo com N11166
31. 89 90 91 maintaining a system log 97 105 maintaining the instrument 47 73 74 97 103 124 Thermo Scientific Wellwash Versa User Manual maintenance 3 47 73 74 97 98 103 105 checklist 97 menu 47 73 memory stick 17 72 menu 35 39 41 43 46 47 48 90 92 layout 47 microplate requirements 59 move wash head 74 80 81 93 normal 62 63 100 102 0 on off switch 32 operational check 33 35 98 ordering information 3 117 orientation 34 39 114 P packing 21 22 104 105 106 for service 21 105 instructions 105 list 21 materials 106 pause 60 66 performing 3 22 34 35 98 103 self diagnostics 34 plate 3 13 17 22 34 37 39 40 41 42 47 50 57 58 59 61 62 63 64 65 66 70 83 84 85 86 97 99 100 102 103 104 105 107 108 111 113 114 118 123 carrier 17 22 34 37 39 40 59 70 84 85 97 99 100 102 103 104 114 clamp 17 40 100 mode 61 orientation 114 type 39 62 63 107 portrait orientation 13 predispense volume 69 preparing soak 74 75 77 79 prime 34 35 37 40 41 43 46 47 60 65 66 68 69 73 93 101 107 108 amp rinse parameters 41 43 47 68 69 volume 41 66 69 108 priming 24 30 35 37 38 40 41 65 66 74 75 76 77 78 79 80 81 82 84 93 102 117 Thermo Fisher Scientific vessel 24 66 74 75 76 78 79 80 81 82 93 102 117 program overview 47 proto
32. B 2 kV line to ground 1 kV line to line Criteria B 3 Vims 190 kHz 80 MHz Criteria A 3 A m Criteria A 30 500 ms Criteria B 60 100 ms Criteria B 100 10 ms Criteria C 100 20 ms Criteria C 100 5000 ms Criteria C EN ISO 12100 1 2003 A1 2009 EN ISO 12100 2 2003 A1 2009 with relation to ISO 14971 2007 Thermo Fisher Scientific warning codes Code 2 3 4 Thermo Fisher Scientific Error and Explanation Chapter 11 Troubleshooting Guide Note Do not use the instrument if it appears to be malfunctioning A Note The instrument does not verify the logic flow of the received commands A When an error is detected the current operation is terminated After an error it is best to abort the current run and restart from the beginning after the problem is fixed To abort press the STOP key after an error and accept by pressing the OK key The error Table 11 9 and warning codes Table 11 10 are presented below Table 11 9 Error codes reported Suggested action Computer command not recognized Invalid computer command argument Plate position error Head position error Check the carrier movement Contact authorized technical service Check the head movement Contact authorized technical service Dispense pump rotation error The pump is jammed Dispense pump time out Plate was not detected Contact authorized technical service The pump is jammed Contact authorized te
33. Fisher Scientific Thermo Scientific Wellwash Versa User Manual 45 Operating the Instrument Display and keys for navigating and editing ES Ea E3 Help 46 Thermo Scientific Wellwash Versa User Manual Use the F1 F3 keys to select a corresponding action shown on the info text bar Figure 5 23 The function assigned to a key depends on the active menu For example the keys can be used to accept or cancel changes close a window add or delete protocol steps sort lists and lock protocols In the Main menu the F1 F3 function keys are reserved for protocols that you can assign to the keys for a quick selection The instrument is shipped with three demo protocols assigned to the keys To assign your own protocols to the keys press the FILE key in the protocol list and select Quick Key gt Set Clear F1 F3 Press the FILE key for example to save the active protocol in the Main menu Depending on the active menu the FILE key opens a list of actions possible for the current protocol New Open Save Save As Quick Key Export Import and Delete Press the HELP key to view more detailed instructions about the context Press the PRIME key to prime the instrument Press the RINSE key to rinse the instrument Press the START key to start the execution of the currently selected protocol Press the STOP key to terminate the active protocol execution Pressing the key also returns the internal software to the previous state
34. OR Press the FILE key in the Main menu and select Open using the Down arrow key and then press the OK key 2 Select the ready made protocol you want to run from the protocol list using the Down arrow key and then press the OK key Selecting the wash head defines the plate type and orientation Table 4 2 Wash head vs plate and orientation Wash head nx8 X12 1x 16 Plate 96 well plate 96 well plate 384 well plate Orientation Landscape Portrait Landscape Insert the 96 or 384 well plate to be washed onto the plate carrier Figure 4 22 Ensure that the microplate is correctly oriented to match Thermo Scientific Wellwash Versa User Manual 39 Running Wash Protocols Priming the system Priming the system 40 Thermo Scientific Wellwash Versa User Manual the wash heads Table 4 2 Position A1 of the plate should correspond to position Al marked on the plate carrier Do not push the plate clamp inward The plate or microstrips must be properly and steadily lowered onto the surface of the plate carrier to prevent jamming of the head movement The plate clamp will apply pressure to the plate to stop free movement during processing Figure 4 22 Inserting the microplate Caution Make sure that the microplate position A1 is inserted correctly before starting wash protocols A Caution Make sure that the strips in strip plates are positioned in the microplate as selected in the user interface otherwis
35. ak wash 2 Press the OK key to empty the priming vessel The priming vessel is emptied 3 Press the OK key when the procedure is done Calibration All the calibration procedures are set in the Calibration window Active calibration 82 Thermo Scientific Wellwash Versa User Manual aa Maintenance Calibration Active calibration Calibrate current wash head al Remove The following calibrations are available e Active calibration see Active calibration on page 82 e Calibrate current wash head see Calibrate current wash head on page 83 You can select either Factory or User e Factory Factory default calibration for the wash head selected in the Settings e User An actual calibration of the wash head performed by a user The date of the last user defined calibration is shown This may have also been performed at the factory Thermo Fisher Scientific Calibrate current wash head Thermo Fisher Scientific Operating the Instrument Maintenance menu The gravimetric calibration is performed in the Calibrate current wash head window It is recommended to calibrate the wash head when the dispensed volume is incorrect or when needed Calibration principle Gravimetric 2 point linear regression curve at 100 pl and 300 ul The valve times in the calibration are based on the factory default values of the wash head Two plates are filled with 100 pl and other two with
36. am layer and anti foaming agent During operation the see through aerosol cover can be present Figure 2 2 or absent The aerosol cover primarily protects the user against biohazardous aerosols and also against environmental contamination The aerosol cover reduces the amount of airborne pathogens making the air you breathe healthier and cleaner Figure 3 20 shows the aerosol cover being installed The corners have magnets for quick magnetic mounting of the cover a EE EI Es a a a d DPDT PTTE TERS Figure 3 20 Magnetic mounting of the aerosol cover To connect the power supply cable Warning Never operate your instrument from a power outlet that has no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region A Thermo Scientific Wellwash Versa User Manual 31 Installation Connecting to a computer USB port for memory stick ON OFF switch Power supply connector Connecting to a computer Warnings and cautions Electrical 32 Thermo Scientific Wellwash Versa User Manual 1 Ensure that the ON OFF switch Figure 3 21 at the right side panel of the instrument is in the OFF 0 position 2 Connect the power supply to a correctly installed line power outlet that has a protective grounded conductor 3 Connect the power supply cable to the power input socket Figure 3 21 at the back of the instrument Figure 3 21 Conn
37. as been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 119 Certificate of Decontamination 120 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Glossary decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination disinfection The destruction of pathogenic bacteria usually with an antiseptic chemical or disinfectant EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions ELISA Abbreviation for enzyme linked immunosorbent assay remote control Running mode allowing a remote computer to operate the washer wash head Interchangeable twin strip 2 x 8 way 2 x 12 way or 1 x blanking heads allowing either 1 x 8 2 x 8 1 x 12 or 2 x 12 processing A single 1 x 16 way wash head allows processing of a 384 well plate USB Universal serial bus Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 121 Glossary 122 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Index A accessories 15 21 117
38. ating the Instrument Main menu ox 2 Select New and press the OK key 3 Select the Head Plate row using the Down arrow key and press the OK key The wash head plate list box appears Refer to Head Plate on page 57 4 Select the wash head and plate with the Down arrow key and or press the OK key to accept the selection If the selected wash head is different from the one that is set in the Settings menu you must change the wash head in the Settings menu Refer to Wash head configuration on page 70 Yell bottom Well offset Select the Well row and press the OK key The Well type window opens Refer to Well on page 58 Select the well bottom shape of the plate or strips with the Down arrow key and or press the OK key to accept the selection 50 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific VAI VE 7 Operating the Instrument Main menu Adjust the well offset value with the Down or Up arrow key Press the OK key to accept the selection For more information on the Preview function refer to Well on page 58 Select the Steps row in the Main menu using the Down arrow key and press the OK key Refer to Steps on page 60 Steps amp parameters No steps Steps amp parameters No steps Steps amp parameters Af 300uI 3 cyc 00 00 Delete Steps are listed and numbered in the order of execution It is poss
39. bration OR Press the F2 key to cancel the calibration OR Press the F3 key to repeat the calibration if the calibration accuracy is not good enough The accuracy must be 2 5 3 5 x that of 100 ul Maintenance Calibrate current wash head Weight Yolume Accuracy Plate 1 9 6000 100 00 000 Plate 2 28 8000 300 00 000 Accept Cancel 11 Calibration is now ready Press the F1 key to accept the new calibration The calibration for the wash head is saved and it becomes the active calibration Thermo Fisher Scientific Operating the Instrument Maintenance menu 12 Press the OK key when the procedure is done from the original factory default values A Note The accuracy indicates how much the current performance differs After the calibration verify the new performance by dispensing a plate for example with 300 pl Weigh the plate to determine the accuracy Service All the relevant service procedures are set in the Service window fi Maintenance Service Dispense speed calibration The Service window has the following parameters Dispense speed calibration Calibrates the dispensing pump speed Calibrate dispensing The dispensing pump speed calibration is performed in the Service pump speed window Principle e Dispensing pump speeds over the required operating range are automatically calibrated using the liquid in the buffer A bottle e Calibration is always performed with
40. can be connected to plate handling devices The Wellwash Versa can be used to wash and prepare plates for a variety of test routines mainly in enzyme linked immunosorbent assay ELISA tests The Wellwash Versa is available in the following configurations e Wellwash Versa 100 240V Cat no 5165010 e 96 and 384 well plate washing e Wellwash Versa 100 240V Fisher Scientific Cat no 5165030 e 96 and 384 well plate washing Figure 1 1 Wellwash Versa microplate washer The Wellwash Versa is a microplate washer intended for automated washing aspiration dispensing and shaking of 96 and 384 well plates and strips that meet ANSI SBS standards The instrument can be Thermo Scientific Wellwash Versa User Manual 13 Introduction to the Wellwash Versa Microplate Washer Intended use equipped with up to four liquid containers It can be used in research or routine test laboratories by professional personnel The Wellwash Versa is part of an analyzing system for the end user who is thus responsible for validation of the whole system in order to produce reliable and safe results If the analyzing performance is critical for medical diagnosis the diagnostic test result has to be checked using internal quality controls or with a corresponding test We recommend using Good Laboratory Practices GLP during the analyzing process Use for self testing is excluded 14 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scien
41. chnical service Plate sensor doesn t work Non volatile parameters lost Contact authorized technical service Attempt to reset the serial number No more memory for storing user data Memory is full delete unused protocols Error s during startup USBwiz error Contact authorized technical service Contact authorized technical service No firmware on the USBwiz chip Contact authorized technical service XY offset adjustment failed Thermo Scientific Wellwash Versa User Manual 111 Troubleshooting Guide Error and warning codes Code Explanation 23 More than one command has the same hash value 20 Parameter memory not found 26 Parameter memory erase failure 27 Parameter memory write failure 50 File open error File not found 53 The file already exists 54 The file does not exist 55 The media is full 56 End of file 57 Other file error none of the previous 58 Firmware update aborted by user 99 File read failed 60 File not found 61 USB memory stick not present 62 File write failed 70 No enough memory 71 Adding the step not successful 80 Invalid step parameter s 81 Too many step parameters 82 At least one of the step parameters is missing 83 Step list is not empty 84 Creating a new step list failed 85 No strips are selected 86 Invalid SOF parameter 91 USB device timeout Suggested action Contact authorized technical service Contact authorized technical service
42. clean the plate carrier Caution Do not disassemble the plate carrier A 1 Turn the power OFF and unplug the instrument 2 Use disposable powder free gloves 3 If spillages have occurred move the plate carrier by pushing it to the left Figure 9 26 This makes it easier for you to clean the area under the plate carrier Thermo Scientific Wellwash Versa User Manual 99 Maintenance Cleaning liquid containers 4 Wipe both the plate carrier and the area surrounding it with a mild detergent 5 Clean the plate carrier immediately when spillages have occurred onto or around the plate carrier Be particularly careful of the area close to the plate clamp a EU TE A E o an v mamaaa Figure 9 26 Cleaning the plate carrier and the surrounding area 6 Move the plate carrier back to its normal position once it has been cleaned Cleaning liquid Before cleaning the waste container empty it according to disposal containers regulations see Disposal of materials on page 101 The containers must be cleaned regularly depending on the applications using a mild detergent The liquid containers tubes and tube connectors can be autoclaved some five to ten times The liquid level sensors or the liquid filters cannot be autoclaved 100 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Can be gt autoclaved Replacing the wash head s Disposal of materials Thermo Fisher Scientific Ma
43. col 26 33 34 35 37 38 39 40 42 46 47 48 49 52 53 55 56 60 63 64 65 66 67 68 70 72 73 89 90 91 92 114 Q quality control 14 Quick Reference Guide 117 ready made protocols 38 reagent 33 61 64 66 70 74 refitting the transport lock 103 105 releasing the transport lock 22 remote control 17 32 46 123 replacing the wash head 97 101 reports 47 68 72 73 residual 108 113 volume 108 113 rinse 27 28 29 33 37 43 46 48 61 64 66 68 69 70 13374 79518599 97 98 107 108 117 time 108 volume 43 69 108 rinsing 27 37 43 93 S self diagnostics 34 sensors 17 29 70 73 111 service 17 21 33 47 73 87 95 97 98 103 105 106 113 117 Settings menu 26 35 68 70 71 83 shake 35 61 65 speed 61 65 shaker 19 107 108 shaking 13 19 65 shutdown 93 97 shutting down 93 97 soak 35 60 61 65 69 74 75 77 78 80 81 93 108 time 61 65 74 75 77 78 108 wash head 69 74 78 93 soaking 13 74 75 77 78 79 81 spare parts 117 specifications 3 107 general 107 performance 108 Thermo Fisher Scientific safety 106 109 speed 19 61 62 63 65 87 88 108 star 40 59 62 118 starting a ready made protocol 38 startup 34 111 status 73 report 73 step 35 40 51 52 54 55 56 57 58 60 61 63 64 65 66 67 68 112 113 steps 35 46 47 51 52 53 54 55 59 60 63 64 65 66 91 stri
44. crements The aspiration height varies according to the plate type and well shape see Table 5 5 on page 62 The default is 2 6 mm Note The aspiration height defines the amount of residual volume in the wells If you want the wells completely empty move the tips all the way to the well bottom a e Aspirate speed You can define the aspiration rate as Low Medium or High The default is High in 96 well format and Low with 384 well format e Aspirate time s The aspiration time ranges from O s to 10 sin 1 s increments The default is J s e Strip over mode You can define the strip over function The default is No e No All selected strips are processed as fast as possible e Yes If Yes is selected unused strips are processed dry The time parameters are measured during the first protocol run Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 63 Operating the Instrument Main menu The wash head stays above unused strips for as long as it takes to process each selected strip Dispense A dispense step fills the wells with liquid The dispense step can be used in a protocol together with other steps W 64 Thermo Scientific Wellwash Versa User Manual Dispense parameters Dispense volume pl 300 Dispense height end 17 0 Strip over mode No The Dispense step has the following parameters Buffer source You can define the reagent source channel A B or Rinse The
45. dispensing height for increased washing efficiency Wells can be left empty or full at the end of the wash step The wash step is the most commonly used step in wash protocols It is often the only step in wash protocols and therefore has more parameters than the other steps Wash parameters Buffer source Ay Wash volume pl 300 More Wash cycles 3 parameters Soak time mm ss Shake speed The wash parameters are grouped into two windows Use the Right and Left arrow keys to move from one window to another Thermo Fisher Scientific Thermo Fisher Scientific Operating the Instrument Main menu Wash parameters cont Wash mode 266 ASpirate time s Strip over mode No Dispense height start Aspirate mode sweep2 Dispense height end Aspirate height 2 6 Final aspirate Aspirate speed The Wash step has the following parameters Buffer source You can define the reagent source channel A B or Rinse The default is A Wash volume pl Volume of the wash buffer used in microplate washing For a 96 well plate the wash volume range is 50 pl to 1000 pl in 10 pl increments and the default value is 300 yl For a 384 well plate the wash volume range is 20 pl to 300 pl in 10 ul increments and the default value is 00 wl It is possible to dispense more than the well volume and the excess liquid is aspirated If a so called overflow volume is selected where the dispensing volume is greater than th
46. dure should be performed if the instrument is left to stand or is switched off at the end of operation If the instrument will be left to stand for a short time during the same day the wash head can be immersed in wash buffer or distilled water Refer to Soak wash head on page 78 and Move wash head on page 80 Caution If the wash head is not rinsed the tips may become clogged If this occurs the wash head will need repairs or may have to be replaced A 3 If the instrument will be left to stand for a longer time priming must be performed to remove all liquid from the system For this purpose remove all tubes from the liquid containers Prime the instrument without liquid to empty all liquid from the system 4 Switch OFF the instrument 5 Ifyou have spilt infectious agents on the instrument disinfect with 70 ethanol or another disinfectant see Decontamination procedure on page 102 Warning The wash heads and priming vessel may be infectious after the instrument has been used A Warning When handling waste containers it is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination A Thermo Scientific Wellwash Versa User Manual 93 Shutdown shutting down 94 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Chapter 8 Emergency Situations Handling I
47. e Wellwash Versa instrument and accessories carefully with the arrows on the transport package pointing upwards Place the instrument onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will invalidate the instrument warranty A Retain the original packaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Using other packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use If you relocate your instrument or ship it for service refer to Packing for service on page 105 Check the enclosed packing list against the order Visually inspect the transport package the instrument and the accessories for any possible transport damage If any parts are missing or damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Thermo Scientific Wellwash Versa User Manual 21 Installation Installation setups Environmental requirements Installation setups Releasing the transport lock 22 Thermo Scientific Wellwash Versa User Manual When you set up your Wellwash Versa avoid sites with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Make sure that e T
48. e and press the F1 Delete key Delete Close New step F 2 Press the OK key to confirm the deletion 56 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Thermo Fisher Scientific Head Plate Operating the Instrument Main menu You can select the wash head and plate format on the Head Plate row in the Main menu If you change the wash head and plate check the step parameters afterwards The default wash head setting is 2 x 8 for Wellwash Versa When a one column wash head 1 x 8 or 1 x 12 is used the blank wash head should always be on the left of the wash arm viewed from the front Press the OK key to accept the selected wash head and plate Thermo Scientific Wellwash Versa User Manual 57 Operating the Instrument Main menu Well JE You can select the well shape and well offset on the Well row in the Main menu If you change the well shape check the step parameters afterwards When you press the OK key the Well type window appears Well type Well bottom Well offset Cancel The following settings are available e Well bottom Select the well shape Different well shapes of strips and plates available are shown in Figure 5 24 V is typically V shaped and differs distinctly from U and C wells The default well shape format is Fiat Note If a 384 well plate is selected you can select only the Flat well shape A Note The well
49. e spilling can occur and the instrument may become contaminated A Caution For proper performance all wells in a strip should have an equal amount of liquid A The PRIME key starts a sequence and a list of buffer channels appears The default channel is the liquid specified by the first step of the wash protocol After selecting the channel set the priming volume by modifying the default volume of 30 ul Priming ensures that the lines to the wash head are filled with liquid If you prime for the first time the lines are empty and you should use a greater volume to fill the lines completely At least 30 ml of liquid is needed to fill the lines from the bottle to the tip On subsequent priming occasions when the lines have liquid you can use a smaller priming volume Note In case of two buffer protocols both the buffer channels have to be primed before the protocol is started If channels are switched within Thermo Fisher Scientific Strip selection Strip selection with the number keys abc def ghi ik mno pars tuv 1 2 a E eon e ze e8 Thermo Fisher Scientific wxyz 0 c 97 10 14 12 Running Wash Protocols Strip selection the same protocol add a prime step to the protocol to ensure that liquid in the tubing is replaced completely volume of at least 15 ml is required A The volume used during priming is defined in the Settings gt Prime amp rinse parameters gt Prime volume Prime button ml menu
50. e well volume the dispensing height is affected That is it is not possible to lift the wash head too high without aspirating the excess liquid Wash cycles You can define the number of wash cycles that will be performed from 1 to 10 The default value is 3 Soak time mm ss The soak time is the time period between wash cycles in the wash step The default value is 00 00 no soak The minimum value is 1 s and the maximum value 60 min in increments of 1 s Shake speed You can define the shake speed Off Low Medium or High The default is Off The shake speed setting is effective only when the soak time is gt 00 00 Caution Ensure that the liquid does not spill at the shake speed and volume settings that you use A Wash mode You can define the wash mode The default is Plate mode e Plate All selected strips are processed before the next cycle Strip Each selected strip is processed the number of cycles defined before moving to the next strip Strip over mode You can define the strip over function The default is Vo e No All selected strips are processed as fast as possible Thermo Scientific Wellwash Versa User Manual 61 Operating the Instrument Main menu 62 Thermo Scientific Wellwash Versa User Manual e Yes Unused strips are processed dry The time parameters are measured during the first protocol run The wash head stays above unused strips for as long as it takes to proce
51. ecifications without prior notice as part of our continuous product development program Table 10 7 and Table 10 8 Table 10 7 General specifications General specifications Overall dimensions Weight Operating conditions Transportation conditions Storage conditions Mains power supply Power consumption Heat dissipation Display Keypad User interface Computer interface Plate types Shaker Number of buffers ca 345 mm W x 385 mm D x 240 mm H 13 6 W x 15 2 D x 9 4 H 9 kg 19 8 Ibs 10 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only 40 C to 70 C packed in transport packaging 25 C to 50 C packed in transport packaging 100 240 Vac 50 60 Hz nominal 100 VA max 341 BTU max High contrast color display with 480 x 272 dots Four arrow keys OK key three function keys F1 F3 FILE and HELP keys 0 12 number keys a z letters and space with the number keys c key START STOP PRIME and RINSE keys Graphical user interface USB 1 1 2 0 compatible RS 232 96 and 384 well plates Linear shaking 3 speeds Low 5 Hz 2 5 mm Medium 10 Hz 1 5 mm and High 15 Hz 1 mm 2 buffers and 1 rinse buffer Thermo Scientific Wellwash Versa User Manual 107 Technical Specifications Performance specifications Performance This section provides the performance specifications for
52. ecting the power supply cable If you are using an external computer with the Wellwash Versa in remote control connect the communication cable to the USB port for PC or serial RS 232 C port Figure 3 21 This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety Ensure that the power supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The power plug should only be inserted into a socket outlet with a p plug y protective ground contact Never use an extension cable without a protective ground wire Thermo Fisher Scientific A A Defects and abnormal stresses A Operating precautions and limitations before operation Thermo Fisher Scientific Installation Operating precautions and limitations before operation Warning Only authorized technical service personnel are allowed to open the instrument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening the instrument A The same precautions applicable when using any electrical equipment should naturally be observed with this instrument Warning Do not touch switches or electrical outlets with wet hands Switch the instrument OFF before disconnecting it from the
53. ensing height A Dispense height start The dispense height start ranges from 0 mm to 17 mm in 0 1 mm increments The default is 6 0 mm Ensure that the value fits the plate in use Because dispensing starts from a lower level in a well and ends at a higher level the start height should be lower than or equal to the end height Dispense height end The dispense height end ranges from 0 mm to 17 mm in 0 1 mm increments The default is 14 4 mm Thermo Fisher Scientific Operating the Instrument Main menu e Final aspirate The wells are emptied in the end of the step The default is Yes which leaves wells empty No leaves the wells wet Aspirate An aspirate step removes liquid from the wells The aspirate step can be used in a protocol together with other steps Aspirate parameters Aspirate mode miu ASpirate height mm Aspirate speed Aspirate time s Strip over mode The Aspirate step has the following parameters e Aspirate mode You can define the aspiration mode Normal Sweep 2 or Sweep 3 The default is Normal Sweep is allowed for 96 well plates with flat or C shaped wells e Normal There is one aspiration position in the center of the well e Sweep2 Aspirates in two different positions across the bottom of the well e Sweep3 Aspirates in three different positions across the bottom of the well e Aspirate height mm The aspiration height ranges from 0 mm to 14 mm in 0 1 mm in
54. es 38 DECLINE anais a EA E 38 Selecting a ready made protocol ssssssesesesesssseserssssesssrsssresssesses 38 Nadi tie Piare aa a aie ewenin sean ae aE 39 PE a oko E ne Rpm rrr nee Penner Pn er ener are ter One ere tee ae ene ree 40 SPSE TECTO Reem ea en ene tree nr eee E eee rte ee ere 4 Strip selection with the number keys cccceessecceeeseeeeeeneeeeeenees 4 Strip selection with the Layout row eeccceesscceeseeceeseeeeneeeeeneeeeees 42 Cance ino a TU Dnne tecnica a a ua ataeae 42 Rinsing the liguid systeameicin a a ieeeneteuteies 43 Chapter 5 Operating the Instrument ssssssessrsssrenuuensnnnunnnnnnunnnunnnunnnnennnennnennnnnnnnnnns 45 Display and keys for navigating and editing 0 0 eee eeeeeeeeeeeeeeeees 45 IVA UNUIS E E carers waeg T T O S ede a neniv as ae rea 47 DY Maile UID sacs teach ety panei ES E E ES A S A 47 Wich di level decin E E 48 Protocolar a e A E S EE 48 Creatine a Protocol nissana a E E nr nase elas 49 Creating a protocol in an optional way esesesssesesesssressresseress 53 Adding new steps to protocols sssssssssssessssesssesssesersssresersseess 54 Deleting steps from protocols ssssssssssssesssesssressresssressresssesses 56 Fead PITS epar E E O 57 A I E E Nats la sect A E E E tal 58 Microplate reguiremMents sresrecninrr rin r o EE E ES 59 STE D E E E E E E EE E coe TE 60 TW AIS Updos E E E eae A EE E EA E E ET 60 E EEE I E T E E A A T A E E A 63 DiD S O A T E E 6
55. ext proto c ol as text file has information about the procol such as the name the steps and their parameters modification date wash head well type and well fi le offset To export a protocol as a text file 1 Insert the USB memory stick Figure 6 25 2 Go to the Protocol row in the Main menu and press the OK key The list of protocols appears Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 91 Exporting and Importing Exporting a protocol as text file 92 Thermo Scientific Wellwash Versa User Manual Protocol Create new protocol Demo1 2x6 96 Demo2 2x8 96 Demo3 Sort Cancel Modified 04 03 2010 11 35 04 03 2010 13 50 04 03 2010 11 36 3 Select the protocol you want to export with the Down arrow key 4 Press the FILE key to open the File menu Protocol Create new protocol Demo1 Demo2 Demo3 Modified 04 03 2010 11 35 04 03 2010 13 50 04 03 2010 11 36 5 Select ASCII export with the Down arrow key and press the OK key The protocol is exported to the USB memory stick You can open the file with a text editor print it or save it Thermo Fisher Scientific Shutting down Thermo Fisher Scientific Chapter 7 Shutdown To shut down the Wellwash Versa 1 Remove any plates still in the instrument 2 Rinse the liquid system with distilled water to remove any buffer solution from the liquid lines and wash head The rinsing proce
56. fic Wellwash Versa User Manual 75 Operating the Instrument Maintenance menu Maintenance Cleaning Disi Clean wash he Empty all bottles Soak wash he OK Move wash he Empty priming vessel 6 Empty all containers and press the OK key Priming is then carried out Maintenance Cleaning Disi Clean wash hi Cleaning procedure completed soak wash he Move wash he Empty priming vessel OK 7 Press the OK key when the procedure is ready Clean wash head The procedure cleans the wash head according to a set of actions when necessary Carry out this procedure if the wash heads are dirty or clogged The instrument fills the priming vessel with a selected liquid and soaks the wash head for a selected period of time Note Use a suitable cleaning agent and repeat the procedure with at distilled water A To clean the wash head 1 Select the Clean wash head row using the Down arrow key and OK press the OK key 76 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Maintenance menu Maintenance X j istilled water Empty priming vessel 2 Change the wash buffer to cleaning agent or distilled water Press the OK key 0 64 Maintenance Clean Cleaning Disinfection procedure Clean wash head soak wash head Move wash head Empty priming vessel Cancel OK 3 Select the priming channel using the Down arrow key and or press t
57. for a 120 V supply Thermo Scientific Wellwash Versa User Manual 109 Technical Specifications Safety specifications 110 Thermo Scientific Wellwash Versa User Manual 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation A EMC performance EN 61000 6 3 2007 EN 61000 6 1 2007 EN 61326 1 2006 EN 61326 2 6 2006 Test standards EN 55011 2007 EN 61000 3 2 2006 Generic standards Emission standard for residential commercial and light industrial environments Generic standards Immunity standard for residential commercial and light industrial environments Product family standard Electrical equipment for measurement control and laboratory use Product family standard Electrical equipment for measurement control and laboratory use in vitro diagnostic IVD medical equipment Performance limits Class B 150 kHz 1 GHz Class A EN 61000 3 3 1995 A1 2001 A2 2005 ANSI C63 4 2003 Class B 450 kHz 1 GHz EN 61000 4 2 1995 A1 1998 A2 2001 4kVCD 8kVAD Criteria B EN 61000 4 3 2002 A1 2002 EN 61000 4 4 2004 EN 61000 4 5 2006 EN 61000 4 6 2007 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 2004 Risk management 3 V m 80 MHz 2 7 Ghz Criteria A 1 kV Criteria
58. g the instrument on page 98 Clean the plate carrier when necessary See Cleaning the plate carrier on page 99 Clean the liquid containers regularly See Cleaning liquid containers on page 100 Replace the wash head when necessary See Replacing the wash head s on page 101 Ensure proper shutdown See Chapter 8 Shutdown Decontaminate the instrument when relocating the instrument or sending it for service See Decontamination procedure on page 102 Service the instrument regularly See Cleaning the instrument on page 98 and Maintaining a system log on page 105 vV depending on the laboratory conditions and the use and configuration of the instrument SIOS ON Daily Weekly Monthly This chapter contains an outline of the points mentioned in the checklist checklist below Table 9 6 Yearly v Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary If needed Thermo Scientific Wellwash Versa User Manual 97 Maintenance Cleaning the instrument Instrument care gt gt gt e Cleaning the Instrument 98 Thermo Scientific Wellwash Versa User Manual Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied Always ensure that the electrical supply in the laboratory conf
59. he OK key Maintenance Clean Cleaning Disir Move wash he OK conti nue a 4 Select the soak time using the Right arrow key and number keys The default is 75 min Press the OK key Priming preparing the soak and soaking take place Pama Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 77 Operating the Instrument Maintenance menu Maintenance Clean Cleaning Disinfection procedure Clean wash h Soaking 00 14 38 Soak wash he Move wash head Empty priming vessel Continue than than the set soak time and to continue the procedure Priming is then carried out pre 5 Press the F2 key to stop the soaking if you want a shorter soak time Maintenance Wash head cleaning procedure ready Repeat the procedure with distilled water if cleaning agent has been used Repeat the wash head cleaning procedure with distilled water if cleaning agent has been used OK 6 Press the OK key when the procedure is ready Soak wash head Soaks the wash head according to a set of actions if necessary The wash head is soaked in liquid standby to prevent clogging The instrument fills the priming vessel with a selected liquid and leaves the wash head immersed in the solution This procedure can be used for a shorter period of time between washes using a wash buffer or to soak for an extended period for example overnight using distilled water Rinse channel To soak the wa
60. he working area is flat dry clean and vibration proof and leave additional room for cables covers and so on e The ambient air is clean and free of corrosive vapors smoke and dust e The ambient temperature range is between 10 C 50 F and 40 C 104 F e The humidity is low so that condensation does not occur relative humidity is between 10 and 80 non condensing Caution Do not operate the instrument in an environment where potentially damaging liquids or gases are present A This section describes the procedures that must be carried out before instrument operation Caution Leave the instrument to stand for at least three hours before installing and switching it on so there is no possibility of condensation causing a short circuit A Warning All parts of the instrument that come into contact with potentially infectious materials must be treated as potentially infectious areas It is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination when performing cleaning procedures and also when making adjustments to the instrument A The instrument comes with one transport lock Caution Make sure that the transport lock has been released before you put the instrument into operation A To release the transport lock 1 Remove the padded packing material protecting the wash head arm and
61. ible to create several steps in the step list and process the steps in the defined Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 51 Operating the Instrument Main menu sequence Steps can be used several times in the user defined order In a majority of applications the protocol has only a wash step Protocol Untitled Head Plate 1x8 96 For more information on the step parameters refer to Steps on page 60 AAAAAAAAAMAMMAMA 11 Select the strips to be processed by using the number keys Refer to Strip selection with the number keys on page 41 OR v ox Use the Down arrow key to select the Layout row in the Main menu and press the OK key Refer to Strip selection with the Layout row on page 42 12 Once the processed area is selected save the new protocol Press the FILE key in the Main menu Note that the processed area is not l saved with the protocol Use the layout step if you want to save the processed area Select Save As using the Down arrow key and press the OK key The Save Protocol As dialog opens 14 Enter the protocol name for example Test by using the number ox and letter keys and then press the OK key The protocol is now to created Note You cannot use the protocol name Untitled j r j bo 3 a oy oe Pre ay a E oF 52 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrume
62. id unnecessary priming with air A Thermo Scientific Wellwash Versa User Manual 65 Operating the Instrument Main menu Pause Jil 66 Thermo Scientific Wellwash Versa User Manual Prime parameters Buffer source A Prime volume ml 30 The Prime step has the following parameter e Buffer source You can define the reagent source channel A B or Rinse The default is A e Prime volume ml The prime volume ranges from 5 ml to 100 ml in 5 ml increments The default is 30 ml Priming is carried out in the priming position Aspiration is on while priming At the end the priming vessel is aspirated empty and the wash head is driven to the home position You can stop a protocol momentarily and then start the protocol again The Pause step can be used in the protocol together with other steps Pause parameters Comment The Pause step has the following parameters e Buzzer You can set the buzzer ON Yes or OFF No There is a beep when the Pause step starts The default is No e Comment You can add text such as instructions There is space for 256 characters To end the pause step press the OK key to continue the protocol execution A three second alarm is sounded The Layout step lets you select strips on the plate This step determines the area on which the next steps are processed The layout is landscape when a 1 x 8 2 x 8 or 1 x 16 wash head is selected and portrait when a 1 x
63. ific Wellwash Versa User Manual Thermo Fisher Scientific Wellwash Versa List of spare parts and accessories Thermo Fisher Scientific Chapter 12 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Table 12 11 through Table 12 13 Table 12 11 Instrument catalog number Code Item 5165010 5165030 Wellwash Versa Table 12 12 Codes for spare parts and accessories Wellwash Versa Fisher Scientific Code Item N11166 Wellwash Versa User Manual CD N11167 Wellwash Versa User Manual Printed N11165 Wellwash Versa Quick Reference Guide N09541 Priming vessel SW smooth blue N10800 1x8 wash head N10801 1x12 wash head N10803 1x16 wash head Wellwash Versa N10804 Blank wash head Wellwash Versa N10807 2 liter wash bottle A Wellwash Versa N10808 2 liter wash bottle B Wellwash Versa N10809 2 liter rinse bottle Wellwash Versa N10810 4 liter waste bottle square N10811 4 liter wash bottle A round N10812 4 liter wash bottle B round Wellwash Versa N10813 4 liter rinse bottle round Wellwash Versa N10814 9 liter waste bottle rectangular Wellwash Versa N10816 Aerosol cover Wellwash Versa N10818 Bottle stand 2x2 configuration 3x2 bottles 1x4 bottle square Wellwash Versa N10819 Bottle stand 1x4 configuration 3x2 bottles 1x4 bottle square Wellwash Versa Quantity 1 1 1 Thermo Scientific Wellwash Versa User Manua
64. intenance Replacing the wash head s Cannot be autoclaved Warning When handling the waste container it is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination A To replace the wash head s refer to Installing the wash head s on page 24 Warning The wash heads may be infectious after the instrument has been used A Warning Disinfect the wash heads thoroughly before you remove them A Warning Prime the instrument without liquid to empty all liquid from the system before removing the wash heads A Warning When handling the waste container it is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination A Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious material Thermo Scientific Wellwash Versa User Manual 101 Maintenance Decontamination procedure Decontamination procedure A 102 Thermo Scientific Wellwash Versa User Manual Warning The samples can be potentially infectious Dispose of all used plates disposable gloves syringes disposable tips and so on as biohazardous waste Be cautious and always use gloves A Warning Treat the
65. interrupted power supply in order to operate correctly Thermo Fisher Scientific has no responsibility whatsoever for system malfunctions arising from power failures Intended users How to use this user manual For more information Thermo Fisher Scientific About This User Manual The Wellwash Versa microplate washer can be used as standalone in research and routine test laboratories by professional personnel This user manual is for the following instruments Wellwash Versa Cat no 5165010 and 5165030 It has been designed to give you the information you need for e Reviewing safety precautions e Installing the instrument e Navigating in the user interface e Operating the instrument e Programming of wash protocols e Defining of wash parameters e Performing cleaning and maintenance procedures e Troubleshooting the instrument performance This user manual also describes all the features and specifications of the Wellwash Versa instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument Keep the user manual together with the instrument in case you distribute it onwards For the latest information on products and services visit our websites at http www thermoscientific com http www thermoscientific com
66. ions for changing the Li battery are described in the service manual A Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Pollution degree 2 see Safety specifications on page 109 Method of disposal Electronic waste Contaminated waste Infectious waste WEEE symbol Do not treat electrical and electronic equipment as unsorted waste Collect waste from electrical and electronic equipment separately A WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS A Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative 106 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific General specifications Thermo Fisher Scientific Chapter 10 Technical Specifications Thermo Fisher Scientific reserves the right to change any sp
67. isk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A Thermo Scientific Wellwash Versa User Manual 5 About This User Manual safety symbols and markings 6 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Contents STNG GSS ene A ESR 3 How to use this user manual seeneseeneseensseorssecssesreseeresecrssecssesrese 3 Formore oorno ensa o alata a 3 Safety symbol sand markings iisen a 4 Safety symbols and markings used on the Wellwash Versa 4 Warning and other markings used in the documentation 5 Introduction to the Wellwash Versa Microplate Washer 13 ended U Caen e a a E 13 Wellwash Versa Main Parts scssssssssssssssssssssseseseseesesesseeseesess 15 TAS ele 40 EN OW eai ae a EA 15 Wiad Sy SCG Mi CAO TAN eeaeee ESE 17 US Be Oy siek DOr renee hee Aecarey arena RAS 17 NOSIS PC POunecie ahaa ec ec eee 17 PN a as ote es gestae A tneeae Saleen shasta ee neater 17 Wash heads errana dana a a A 17 SACP ean r a a 19 Mmstalatl On oios ae sia eouataer aoe 21 What to doupon delivery seminere e a 21 Unpack me thein irU
68. ispense A 300pl Aspirate Delete 5 Press the OK key to edit the aspirate step For more details on the Aspirate step refer to Aspirate on page 63 Aspirate parameters Aspirate mode Aspirate height mm 2 6 Aspirate speed High Aspirate time s 1 Strip over mode No 6 Save the changes you make in each step 7 Press the F3 New step key to add further new steps to the protocol Steps amp parameters Wash A 300pl 3 cyc 00 00 Dispense A 300pl J5 W Aspirate Cancel 8 Select a new step with the Down arrow key and press the OK key to add the step to the protocol You can continue in the same manner as above adding the desired steps to your protocol When there are more steps present than the display can show blue arrows appear at the bottom and or at the top of the display Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 55 Operating the Instrument Main menu Dispense A 300ul Soak 00 00 Off Wash Af 300ul 3 cyc 00 00 Aspirate Normal Dispense A 300ul Delete Close New step a 9 Use the Up and Down arrow keys to navigate in the protocol Deleting steps from To delete a step from a protocol protocols Steps amp parameters Aspirate Normal W w Dispense A 300ul Je Soak 00 00 Off A 300ul 3 cyc t OD 00 1 Use the Up and Down arrow keys to go to the step you want to delet
69. l 115 Code Item Quantity N10820 Small bottle stand for own bottles 1 N10821 Spare bottle 2 1 N10822 Spare bottle 4 round 1 N04001 USB A B device cable 1 2305290 Serial cable F9 F25 1 longer USB cables available from PC stores List of Thermo Table 12 13 Codes for plates Scientific plates code Item 439454 Nunc solid F96 464718 Nunc F384 430341 Nunc C96 449824 Nunc U96 249662 Nunc V96 441653 Nunc Starwell strip plate 116 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Appendix A System Log Instrument name number User Date Comments o ee a PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 117 System Log 118 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Appendix B Certificate of Decontamination Name Address Tel Fax Instrument Name Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information on contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit h
70. llwash Versa Figure 2 5 Wellwash Versa wash head models Blank head 1x8 head 1x8 96 Blank head 1312 head 1x12 96 1x6 head 1x8 head 2x8 96 1x12 head 1312 head 2x12 96 1x16 head 1x16 384 Figure 2 6 Configurations of Wellwash Versa wash heads 18 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Wellwash Versa Main Parts Shaker Shaker The linear shaker operates at three different speeds Table 2 1 Table 2 1 Shaking speeds Speed designation Speed Low 5 Hz amplitude 2 5 mm Medium 10 Hz amplitude 1 5 mm High 15 Hz amplitude 1 mm Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 19 Wellwash Versa Main Parts Shaker 20 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific A What to do upon delivery Unpacking the Checking delivery for completeness or Thermo Fisher Scientific instrument A damage Chapter 3 Installation This chapter describes the installation procedures for the Wellwash Versa instrument Warning The Wellwash Versa weighs 9 kg 19 8 Ibs and care must be taken when lifting the instrument A This section covers the relevant procedures to be carried out on receipt of the instrument Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective plastic wrapping until the ambient temperature has been reached Unpack th
71. logs 73 messages 42 105 exporting a protocol 89 91 exporting data or protocols 72 89 91 92 F flat 22 58 62 63 108 foam 30 31 foaming 30 31 G gravimetric calibration 83 87 H handling emergency situations 95 Thermo Scientific Wellwash Versa User Manual 123 importing a protocol 90 importing data or protocols 89 90 91 info text bar 38 46 48 installation 3 21 22 24 28 31 33 34 35 101 109 category 109 installing 3 22 24 28 31 101 liquid containers 28 Wellwash Versa 21 22 33 109 instrument settings 68 intended use 3 13 K keypad 45 98 107 113 L landscape orientation 13 17 language 35 72 107 layout 37 41 42 47 52 60 66 67 68 114 editing 41 linear regression 83 linear shaking 107 liquid 14 17 27 28 29 30 34 35 37 38 40 43 48 60 61 63 64 65 70 76 78 80 81 84 85 93 97 98 100 101 103 105 113 114 A sensor 70 B sensor 70 channels 27 35 containers 14 27 28 29 30 34 37 38 48 70 93 97 98 100 103 105 filters 98 100 103 level 27 28 30 34 37 38 48 70 98 100 103 level detection 27 38 48 level sensors 28 30 34 70 98 100 103 Rinse sensor 70 system diagram 17 Waste sensor 70 locked 49 67 68 main 15 37 39 41 42 46 47 48 49 51 52 53 54 57 58 60 67 68 89 90 91 menu 37 39 41 42 46 47 48 49 51 52 53 54 57 58 60 67
72. lters in the liquid tubes are clean A Warning Make sure that the liquid level of the waste bottle is always kept below the maximum level indicated on the bottle to avoid potential overflow as the contents of the waste bottle are potentially infectious A Warning The contents of the waste bottle are potentially infectious so it is important to wear protective clothing such as disposable gloves a laboratory coat and safety glasses when emptying or handling a waste bottle A The liquid level sensor is unable to detect foam In case of foaming 1 When using wash buffers that show a strong tendency of foaming empty the waste container as soon as the foam level has reached the maximum filling level indicated on the waste container Thermo Fisher Scientific Installing the aerosol cover Connecting the power supply cable A Thermo Fisher Scientific Installation Installation setups 2 Additionally add a commercially available anti foaming agent such as silicone oil to the empty waste container to reduce foaming Use concentrations of anti foaming agents as recommended by the manufacturers 3 If foaming continues to be a problem we recommend switching to a larger waste container and additionally increasing the concentration of anti foaming agent in the waste container 4 To help facilitate breakdown of the foam in the waste container carefully swirl the waste container from time to time to improve mixing between fo
73. lwash Versa User Manual Thermo Fisher Scientific Liquid containers Thermo Fisher Scientific and channels me WASTE Venting holes i lt A Installation Installation setups The Wellwash Versa includes three standard buffer containers 2 liters and a waste container 4 liters Containers for greater volumes are available as an option 4 l for buffers and 10 for waste The liquid channels are A for Buffer A B for Buffer B R for Rinse and W for Waste Channel R is primarily intended for rinsing and is connected to the RINSE key as the only channel The Rinse channel can also be used in wash protocols as the buffer source The liquid containers are named and the tubing is color coded to correspond to the correct buffer source A blue B yellow R green and W colorless Figure 3 15 Refer to Connection diagram on page 28 Figure 3 15 Waste and liquid containers with liquid level detection sensors Caution Do not cover the venting holes a Thermo Scientific Wellwash Versa User Manual 27 Installation Installation setups Connection diagram Figure 3 16 shows the connections of the liquid level sensor cabling and liquid container tubing WASTE Figure 3 16 Wellwash Versa connection diagram Installing the liquid To install the liquid containers containers 1 Fit the liquid container tubing to the corresponding color coded tube connectors on the left side panel of
74. m Wash head manifolds Liquid valve manifold gt K a gt L j yf ce arr r f ff ff ff 4 d f Pi L A ff A fy fi Z Dispense valves E A E fh of OF E of of ef F ff FH A SS LF F A EP of ff Pf KOI Sf LF fk T 3 Vy pi Dispense pump R B A I I I Liquid level sensors B w e Aspirate pump Liquid containers 9 Liquid level sensor Waste container The instrument is equipped with a USB port host for an external memory stick Figure 2 4 You can transfer wash protocols from one instrument to another of the same model with the USB memory stick The instrument is equipped with a USB port client for an external PC connection The connection is used for remote control and service of the instrument A separate manual Wellwash Versa Remote Control Command Sets Cat no D08894 is available upon request The plate carrier of the Wellwash Versa instrument supports both portrait and landscape orientation of the plate Figure 2 2 A plate clamp is incorporated into the plate carrier to keep the plate firmly positioned during processing A sensor in the plate clamp senses the presence of a microplate The wash head alternatives Figure 2 5 and their configurations Figure 2 6 are shown below Thermo Scientific Wellwash Versa User Manual 17 Wellwash Versa Main Parts Wash heads 1x8 wash head 1x12 wash head 1x16 wash head Wellwash Versa Blank wash head vve
75. mains supply 4 This section describes defects and abnormal stresses Warning If the instrument is not functioning properly it may create electromagnetic interference which could impair the operation of other devices or equipment in the usual environment A Whenever it is likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation Contact authorized technical service immediately The protection is likely to be impaired if for example the instrument e Shows any visible damage e Fails to perform the intended functions e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses pe Read this manual in its entirety as it contains information necessary to ensure safe operation 2 Always ensure that the electrical supply in the laboratory conforms to that specified on the type label on the rear of the instrument 3 Particular attention should be paid to the correct fitting of the tubing follow the color code carefully 4 Fill the reagent and rinse containers only after the installation and operational check of the system 5 Check that the correct protocol has been selected for the washer Thermo Scientific Wellwash Versa User Manual 33 Installation Switching on 6 Check that the liquids are put in the correct containers called for by the protocol 7 Check that there is
76. mpi http www thermoscientific com readingroom http www thermoscientific com wellwash In our efforts to provide useful and appropriate documentation we appreciate your comments on this user manual to your local Thermo Fisher Scientific representative Thermo Scientific Wellwash Versa User Manual 3 About This User Manual safety symbols and markings Safety symbols and markings Safety symbols and markings used on the Wellwash Versa x Bc HHO 4 Thermo Scientific Wellwash Versa User Manual These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated The following symbols and markings appear on the type label and the instrument itself Power ON a Power OFF a Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A A black label with the following text Figure 2 3 on page 16 CAUTION WARNING DISCONNECT SUPPLY BEFORE SERVICING and AVERTISSEMENT COUPER L ALIMENTATION AVANT L ENTRETIEN ET LE DEPANNAGE 4 Thermo Fisher Scientific Warning and other markings used in the documentation Thermo Fisher Scientific DEEA About This User Manual Safety symbols and markings The following symbols and markings appear in this user manual Warning R
77. must add distilled water or a suitable cleaning agent manually into the priming vessel This procedure can be used to leave the wash head in liquid standby or when a clogged wash head is cleaned and the instrument pump cannot be used to fill the priming vessel To move the wash head 1 Select the Move wash head row using the Down arrow key and press the OK key 80 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Maintenance menu Maintenance Clean Cleaning Disin 2 Fill the priming vessel manually if necessary Press the OK key Preparing to soak and soaking take place Maintenance Clean Cleaning Disinfection procedure Clean wash h Soak wash he 1 Move wash heaa Empty priming vessel Continue 3 Press the F2 key to abort the soaking and to continue the procedure 4 Press the OK key when the procedure is ready Empty priming vessel The procedure empties the priming vessel of liquid The procedure can be carried out for example after the Move wash head procedure Refer to Move wash head on page 80 To empty the priming vessel 1 Select the Empty priming vessel row using the Down arrow key and press the OK key Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 81 Operating the Instrument Maintenance menu 7 Maintenance Clean wash C En SSSR ED Move wash 1 So
78. n case there is any abnormal situation during operation such as fluids emergency spilling inside the instrument situations 1 Switch OFF the instrument Figure 2 4 on page 16 2 Unplug the instrument immediately from the power supply Figure 2 3 on page 16 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 If the corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 95 Emergency Situations Handling emergency situations 96 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Regular and preventative Chapter 9 Maintenance maintenance Maintenance Thermo Fisher Scientific Table 9 6 Maintenance checklist Item Rinse the instrument with deionized distilled water after use See Instrument care on page 98 Keep the instrument free of dust See Instrument care on page 98 Wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Cleaning the instrument on page 98 If any surfaces have been contaminated with biohazardous material disinfect with a mild sterilizing solution See Instrument care on page 98 Clean the case of the instrument periodically See Cleanin
79. n the protocol the Layout row is not locked but enabled You can select strips using the row until the first layout step in the protocol is encountered There are two ways to select strips in the Main menu The first is by selecting the Layout row and the second is by using the number keys Refer to Strip selection with the Layout row on page 42 and to Strip selection with the number keys on page 41 You can specify the instrument settings in the Settings menu The Settings menu contains the Prime amp rinse parameters Sensors Wash head configuration System and Reports parameters Settings Wash head configuration System Reports The prime and rinse parameters can be set in the Prime amp rinse parameters window Go to the Prime amp rinse parameters row in the Settings menu and press the OK key Thermo Fisher Scientific Operating the Instrument settings menu Settings Prime amp rinse parameters Prime volume Prime button ml Predispense volume ml Rinse volume Rinse button ml Autorinse Autoprime Warning If you activate the Autorinse or Autoprime parameter ensure that the wash heads are firmly attached A The Prime amp rinse parameters window has the following parameters e Prime volume Prime button ml The prime volume ranges from 5 ml to 100 ml in 1 ml increments The default is 30 ml e Predispense volume ml The predispense volume ranges from 1 ml to
80. ne ni cianeiants nn a 21 Checking delivery for completeness or damage ssseeeeeeeseeeseees 21 Environmental Tequite M1 C105 mernes nna aa 22 Installations etU p o EE E etre 22 Releasing tMetrans port lOc Resiceiea a 22 Removing the priming Vessel ccncsscceteczcecssodecgatgedsncadencs cdaceteueeacgevans 24 Installing the wash head s rinner 24 Liquid containers and channels ccisedssinstnsanestenssachstevenetverdavdideardes 27 CGonnecnon dagtiniai E enon RE 28 Installing the liquid containers ssssessseessesesesesseressresseressresses 28 TG AAI 22 Sos n E E TE TNN 30 Installing the actosol cOver scssseiatsntcgvieiseoneneduncevctutiguncuevenieede 31 Connecting the power supply Cable ws ccuasesiceriieaer aunt 31 Connecting toa COM pU an a aca ay 32 WANES ARCAS n apecias ate aa tad tea ent ide Ge reaeeaatagee 32 Fecr Al scp su htt tae taal te Oi ae ae eet en eek ae EL 32 Defects and abnormal stresses c ccsseccccsecccnssccccecceceeeccensccens 33 Operating precautions and limitations before operation 0 33 SW CIS OU eeoa i tate eolacney aie E eee 34 Performing the operational check ees eeeseeeesseeeeneeeesneeeeeaeeeeeaees 35 Settings after installation seseris icons iieiea 35 Thermo Scientific Wellwash Versa User Manual 7 Contents Chapter 4 Running Wash Protocols ccsssssssssesesesesseseeseeseesesseseeseesnsanees 37 Filling and emptying liquid containers s sssesesesessesseessressressrese
81. ng the transport lock Figure 9 27 Aligning the plate carrier and wash head arm 2 Screw on the transport lock screw and the transport lock tag with the supplied Allen key Figure 9 28 p E pog Figure 9 28 Fastening the transport lock 3 Place small plactic bags around the tubes to protect them during transport 4 Insert the padded packing material around the plate carrier and wash head arm for protection after the transport lock is refitted Figure 9 29 104 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Maintaining a system log Packing for service Disposal of the instrument Thermo Fisher Scientific Maintenance Maintaining a system log Figure 9 29 Transport lock and tag fitted A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system can be very useful in properly maintaining the system Refer to Appendix A System Log Copy the table as many times as necessary but leave the blank original inside the user manual To pack the Wellwash Versa for service follow the instructions presented below e Inform Thermo Fisher Scientific about the use of hazardous materials e Remove any microplate before decontamination Decontaminate the instrument Remove the liquid containers and wash heads e Refit the transport lock Refer to Refitting the transport lock on page 103
82. nt Main menu Creating a protocol in an An optional way to create a protocol is to use the Protocol window optional way 1 Select the Protocol row in the Main menu using the Up or Down arrow key and press the OK key 2 Select the Create a new protocol row and press the OK key Protocol Modified Create new protoco 1x6 96 18 02 2010 12 36 1x6 96 18 02 2010 10 33 Sort Cancel Well aie Te 4 7 Protocol Wash2J s E rae ms 4 A i TA 5 OK Save F2 Cancel 4 Inthe Main menu set the desired protocol parameters and steps 5 When ready press the File key and select Save to save your protocol Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 53 Operating the Instrument Main menu Adding new steps to To add a new step to a protocol protocols 1 Select the Steps row in the Main menu using the Down arrow key and press the OK key Steps amp parameters A 300pl 3 cyc 00 00 2 gj Dispense A 300pl v ox 3 Select the step with the Down arrow key and or press the OK key 4 A flashing insert bar with the selected step appears Select the position where you want to insert the step by using the Up or Down arrow key Press the F3 Insert key 54 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Main menu Steps amp parameters 1 Sf Wash A 300pl 3 cyc 00 00 2 g D
83. ntific Operating the Instrument Maintenance menu Maintenance Calibrate current wash head Yolume Accuracy 6 Insert the second tare weighed plate onto the plate carrier and press the F1 key to continue Dispensing is carried out Maintenance Calibrate current wash head 7 Weigh the dispensed plate and enter the mass of the dispensed OK liquid using the number keys Press the OK key when ready to Maintenance i Calibrate current wash head Weight Yolume Accuracy Plate 1 Plate 2 Average Delta 8 Press the F1 key to continue the calibration OR Press the F2 key to cancel the calibration OR Press the F3 key to repeat the calibration if the calibration accuracy is not good enough The accuracy of the first plate must be 50 and that of the second plate must be 5 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 85 Operating the Instrument Maintenance menu 86 Thermo Scientific Wellwash Versa User Manual Maintenance Calibrate current wash head Weight Yolume Accuracy Continue Cancel 9 Continue dispensing and weighing the third and fourth plate in the similar way At the end you should have the mass of both plates entered and their accuracy calculated Maintenance y Calibrate current wash head Weight Yolume Accuracy beta ooo J SS Continue Cancel 10 Press the F1 key to continue the cali
84. nts 2006 42 EC Machinery Directive 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment Safety performance IEC 61010 1 2001 Ed 2 with US and CA National differences IEC 61010 2 101 2002 Ed 1 Particular Requirements for In Vitro Diagnostic IVD Medical Equipment The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation category ll according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V
85. ocols Canceling a run Strip selection with the Layout row Canceling a run 42 Thermo Scientific Wellwash Versa User Manual e Hold the C 12 key down for more than 2 seconds to clear all selections e Double press the C 12 key to select all when the area is empty e Strip selection works when any row in the Main menu is active The layout of the plate or strips in the protocol can alternatively be edited in the Layout window Go to the Layout row in the Main menu and press the OK key Select unselect columns with OK key Move cursor with left right array key or right array key Clear all by pressing C key Select strips by pressing the OK key and by moving with the left or right arrow key Strips 1 12 are edited if an 8 way wash head is used or strips A H if a 12 way wash head is used Select unselect columns with OK key Move cursor with left or right array key Clear all by pressing C key You can cancel a started run by pressing the STOP key except when the Wellwash Versa is already waiting for a user input key to some warning or error message being displayed as a consequence of some error detected in the run Thermo Fisher Scientific Rinsing the liquid system Thermo Fisher Scientific Running Wash Protocols Rinsing the liquid system The RINSE key starts a sequence where the rinse liquid is pumped through the liquid system for a short period of time and left in the line
86. orms to that specified on the type label of the instrument To guarantee the continuous reliability and accuracy of the Wellwash Versa e Rinse the instrument with deionized distilled water after use e Prevent any liquid from entering the instrument e Keep the instrument free of dust and other foreign matter e Perform the operational check regularly see Performing the operational check on page 35 In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Cleaning the instrument on page 98 Clean the keypad surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used A Caution Do not use the instrument to aspirate or dispense any acidic solutions as this could damage the instrument A Caution Never use acetone as it will damage the covers A Caution Do not autoclave any part of this instrument apart from the liquid containers tubes and tube connectors However the liquid level sensors or the liquid filters cannot be autoclaved A Clean the instrument regularly as stated below
87. plate carrier to expose the transport lock and transport lock tag Figure 3 7 Thermo Fisher Scientific Thermo Fisher Scientific Installation Installation setups Figure 3 7 Transport lock and transport lock tag 2 Unscrew the transport lock screw with the supplied Allen key Figure 3 8 Figure 3 8 Untastening the transport lock 3 Keep the transport lock and tag Figure 3 9 for future relocation or transportation of the instrument A Ree Figure 3 9 Transport lock and tag removed Thermo Scientific Wellwash Versa User Manual 23 Installation Installation setups Removing the The instrument comes with the priming vessel installed The priming priming vessel vessel is correctly installed if it stays in place and does not move up To remove the priming vessel 1 Push the priming vessel away from yourself towards the instrument until you hear a snap 2 Lift the priming vessel up and remove it Figure 3 10 Figure 3 10 Removing the priming vessel Warning The priming vessel may be infectious after the instrument has A been used A Installing the wash The wash heads are packed separately and must be installed before use head s Warning The wash heads may be infectious after the instrument has AAN been used A Note Always wear disposable powder free gloves when handling the wash heads A To install the wash head s 1 Fit the wash heads to the tubing according to Figure 3
88. ps selecting with the Layout row 42 selecting with the number keys 37 41 47 52 sweep 62 63 108 switching on 34 symbols 4 system 2 5 14 33 34 35 37 38 40 43 47 65 68 69 71 72 73 93 101 105 119 123 log 105 settings 73 T time 2 31 34 40 43 47 61 62 63 64 65 69 71 72 75 76 78 93 111 format 72 transport 21 22 23 33 103 104 105 107 damage 21 package 21 103 troubleshooting 3 111 113 guide 113 the instrument 113 type label 4 33 98 U unpacking the instrument 21 USB 17 32 34 72 89 90 91 92 107 112 118 123 memory stick 17 34 72 89 90 91 92 112 memory stick port 17 72 PC port 17 user interface 3 45 72 107 W warning codes 111 112 warranty 21 Thermo Scientific Wellwash Versa User Manual 125 wash 3 13 17 18 22 24 25 26 27 30 34 35 37 38 39 40 41 42 47 48 49 50 52 57 59 60 61 64 65 66 67 68 69 70 71 73 76 77 78 79 80 82 83 86 89 91 93 97 99 101 103 104 105 108 113 114 117123 cycles 61 65 108 head 17 18 22 24 25 26 34 35 39 40 41 42 47 49 50 57 59 61 66 67 68 69 70 71 73 74 76 78 80 81 82 83 86 87 91 93 97 101 103 104 105 108 113 114 117 123 head arm 22 103 104 head configuration 26 34 47 68 70 71 73 83 126 Thermo Scientific Wellwash Versa User Manual head format 49 mode 61 protocol 3 1
89. r Refer to Liquid containers on page 27 4 Always reprime the system before running a wash protocol Refer to Priming the system on page 40 You can select a protocol from the protocol list or first create a protocol and then start it Refer to Selecting a ready made protocol on page 38 and Creating a protocol on page 49 You can select a ready made protocol either with the F1 F3 keys or from the protocol list in the display see Protocol on page 48 Three ready made demo or favorite protocols can be connected to the F1 F3 keys to select routine assays quickly To assign your own protocols to the keys press the FILE key in the protocol list and select Quick Key gt Set Clear F1 F3 To start a ready made protocol shown on the info text bar with the quick keys F1 F3 or from the protocol list Thermo Fisher Scientific F1 F3 function keys Example protocol list rox vc Vea Loading the plate Thermo Fisher Scientific Demot 1x6 96 Flat 1 Wash 2 Dispense 11 ZAT Protocol Create new protocol Demo1 Demo2 Demo3 Test1 1x8 96 1x6 96 1x6 96 1x6 96 Well Flat Flat Flat Flat Modified 09 02 2010 13 02 09 02 2010 13 05 09 02 2010 13 03 09 02 2010 12 56 Running Wash Protocols Loading the plate 1 Press for example the F1 key Demo in the Main menu OR Press the OK key on the Protocol row in the Main menu
90. rocedure using the prepared decontaminant Refer to Cleaning Disinfection procedure on page 74 9 If needed autoclave the liquid containers tubes and liquid connectors However the liquid level sensors or the liquid filters cannot be autoclaved 10 Empty the plate carrier Ensure that you are wearing disposable powder free gloves 11 Switch OFF the power and disconnect the power supply cable 12 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol 13 Place the instrument in a large plastic bag 14 Place a cloth soaked in the prepared decontaminant solution into the bag Ensure that the cloth does not come into contact with the instrument 15 Close the bag firmly and leave the instrument in the bag for at least 24 hours 16 Remove the instrument from the bag 17 After decontamination clean the instrument using a mild detergent 18 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package Refitting the When you relocate the instrument or ship it for service make sure that transport lock 7 refit the transport lock To refit the transport lock 1 Align the wash head arm and the plate carrier in order to refit the transport lock screw Figure 9 27 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 103 Maintenance Refitti
91. s e Status report The status report shows the following parameters report name date and time instrument name version and serial number current protocol prime and rinse parameters sensor settings and status wash head configuration system settings calibration parameters for all wash heads offset values and pump usage hours Maintenance You can specify the maintenance procedures in the Maintenance menu Thermo Fisher Scientific me nu The Maintenance menu contains the Clean Calibration and Service parameters Main Settings f Maintenance Ay Clean Calibration Service Clean Cleaning and disinfection procedures are set in the Clean window Maintenance y Clean Clean wash head Soak wash head Move wash head Empty priming vessel Close Remove The Clean window has the following parameters Thermo Scientific Wellwash Versa User Manual 73 Operating the Instrument Maintenance menu e Cleaning Disinfection procedure see Cleaning Disinfection procedure on page 74 e Clean wash head see Clean wash head on page 76 e Soak wash head see Soak wash head on page 78 e Move wash head see Move wash head on page 80 e Empty priming vessel see Empty priming vessel on page 81 Cleaning Disinfection Cleans or disinfects the instrument according to a set of actions Refer procedure to Chapter 10 Maintenance particularly Decontamination procedure on page 1
92. s The volume used during rinsing is defined in the Settings gt Prime amp rinse parameters gt Rinse volume Rinse button ml menu Refer to Prime amp rinse parameters on page 68 Thermo Scientific Wellwash Versa User Manual 43 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 44 Chapter 5 Operating the Instrument This section describes the user interface of the Wellwash Versa instrument Display and keys The keypad and display are shown in Figure 5 23 for navigating and editing Thermo SCIENTIFIC WELLWASH VERSA EMMA Settings Maintenance 13 05 lt 4 Menu bar Active row gt i lemo Head Plate 1x8 96 well Flat START STOP a onnan PRIME and Steps 1 Wash 2 Disp a RINSE keys amp 1 2 3 4 5 6 7 8 9 10 11 12 500000000000 Info text bar __ __ Demol Demo2 Demo3 a P S CA q eo Function keys Numeric keys with letters nm 8aa800000000 Figure 5 23 Keypad and display of the Wellwash Versa The keys for navigating and editing are detailed below The keys also have other functions depending on the menu level in the user interface The active row is colored blue Use the Left Right Up and Down arrow keys to navigate You can repeat the selection by holding down the arrow key lt PAV ox Use the OK key to select and edit the highlighted item It is mostly used for setting and accepting the active parameter Thermo
93. sh head 1 Select the Soak wash head row using the Down arrow key and OK press the OK key 78 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Operating the Instrument Maintenance menu Maintenance 2 Change the wash buffer to cleaning agent or distilled water if necessary Press the OK key OK V Maintenance Clean Cleaning Disinfection procedure Clean wash head soak wash head Move wash head Empty priming vessel Cancel OK 3 Select the priming channel using the Down arrow key and or press the OK key Priming preparing the soak and soaking take place Maintenance Clean Cleaning Disinfection procedure Clean wash h Soaking Soak wash he Move wash hea Empty priming vessel Continue 4 Press the F2 key to abort the soaking and to continue the procedure The priming vessel is emptied Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 79 Operating the Instrument Maintenance menu Maintenance Clean Cleaning Disinfection procedure Clean wash he Emptying priming vessel Soak wash he Move wash head Empty priming vessel 5 Press the OK key when the procedure is ready Maintenance Clean Cleaning Disi i Clean wash hi Procedure done Soak wash he Move wash he Empty priming vessel Move wash head The procedure moves the wash head down into the priming vessel to the soak position You
94. ss each selected strip Aspirate mode You can define the aspiration mode Normal Sweep 2 or Sweep 3 Sweep is allowed for 96 well plates with flat or C shaped wells The default value depends on the wash head type or well shape e Normal There is one aspiration position in the center of the well e Sweep2 Aspirates in two different positions across the bottom of the well e Sweep3 Aspirates in three different positions across the bottom of the well Aspirate height The aspirate height ranges from 0 mm to 14 mm in 0 1 mm increments The default aspirate height varies according to the plate type and well shape Table 5 5 The zero height from which the aspirate height is defined is the lowest level of a plate when the plate is properly placed on the plate carrier Table 5 5 Plate and well shape vs default aspirate height Plate Well shape Default aspirate height 96 Flat 2 6 mm 36 C 2 3 mm Default aspirate heights are 96 U 42mm defined so that the wash heads float above the bottom For J6 V 3 4 mm example 0 5 mm above the 96 Star 314mm bottom with flat bottom plates 384 Flat 2 2mm Aspirate speed You can define the aspiration rate Low Medium or High The default is High Aspirate time s The aspiration time ranges from 0 s to 10s in 1 s increments The default is J s Note Because aspiration is on during dispensing the residual volume in the wells depends on the disp
95. sufficient liquid in the liquid containers and room in the waste container to run the protocol or series of protocols Empty the waste container before a run or a series of runs The liquid level sensor will warn you if safe levels have been passed Some warnings can be overridden at the user s risk 8 Check that the wash head configuration matches the configuration demanded by the protocol For example if 2 x 12 way heads have been fitted and the protocol demands a x 8 way head this may result in the plate carrier getting wet 9 Fit the microplate in the correct orientation appropriate for the wash head configuration Note that if you do not load a microplate correctly onto the instrument for example a 12 way wash head has been fitted when a plate is loaded for an 8 way head this will result in the plate carrier getting wet 10 Select the number of strips to be processed correctly If there are empty strips on a plate ensure that they are not selected to be processed Switching ON Check that all the cables are properly fitted according to the installation instructions Switch the instrument ON The system performs initialization tests self diagnostics each time when switched on Caution Do not switch the power off during Performing self diagnostics Do not plug unplug the USB memory stick during Performing self diagnostics A buffer Ensure that the dispensing pump is not run for longer than a few min
96. the 2 x 8 wash heads e Dispense speed calibration should be performed if the gravimetric calibration fails or after every 10 000 dispensed plates To calibrate the dispensing pump speed 1 Select the Dispense speed calibration row using the Down arrow OK key and press the OK key Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 87 Operating the Instrument Maintenance menu Maintenance Fill the buffer A bottle with distilled water and press the OK key Dispensing pump speed is calibrated Maintenance Dispense speed calibration 88 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Exporting a protocol Thermo Fisher Scientific Chapter 6 Exporting and Importing This section describes how to export or import wash protocols from one instrument to another To export a protocol from the instrument to a USB memory stick 1 Insert the USB memory stick Figure 6 25 DT are MIegggag 7 Figure 6 25 USB memory stick inserted 2 Go to the Protocol row in the Main menu and press the OK key The list of protocols appears Well Modified 1x6 96 Flat 10 02 2010 10 57 1x16 364 Flat 10 02 2010 11 02 v 3 Select the protocol you want to export with the Down arrow key Thermo Scientific Wellwash Versa User Manual 89 Exporting and Importing Importing a protocol OK 4 Press the FILE key to open the File menu Protocol
97. the instrument Figure 3 17 The connectors are from left to right W Waste R Rinse B Buffer B and A Buffer A 28 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Thermo Fisher Scientific Installation Installation setups Figure 3 17 Connecting the liquid container tubing 2 Connect the liquid sensor cables into the appropriate color coded sockets on the left side panel of the instrument Figure 3 18 The sockets are from top to bottom W Waste R Rinse B Buffer B and A Buffer A Figure 3 18 Connecting the liquid sensor cable All the liquid containers are connected to the instrument in Figure 3 19 Thermo Scientific Wellwash Versa User Manual 29 Installation Installation setups Foaming 30 Thermo Scientific Wellwash Versa User Manual lt a x gt SSE Figure 3 19 Liquid containers connected to the instrument Caution Always ensure that the liquid containters are attached properly Always connect the container cap to the correct container and to the correct connector on the left side of the instrument Otherwise the wash performance can be seriously affected A Caution Ensure that the intake tube is all the way down at the bottom so that no red tubing is visible A Caution Ensure that the container of the priming solution is always full at the beginning of the priming procedure A Caution Check regularly that the inlet fi
98. tific Chapter 2 Wellwash Versa Main Parts This chapter describes the main parts of the Wellwash Versa instrument Instrument The front view of the Wellwash Versa instrument is shown in Figure 2 2 views P AN Display v O Liquid containers Rase P a aa lt _ Keypad Wash heads on wash head arm See through aerosol cover Priming vessel Bottle stand option Plate carrier Plate clamp Figure 2 2 Wellwash Versa front view with accessories The back view of the Wellwash Versa instrument is shown in Figure 2 3 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 15 Wellwash Versa Main Parts Instrument views USB port for memory stick ON OFF switch Power supply connector Figure 2 3 Wellwash Versa back view The side views of the Wellwash Versa instrument are shown in Figure 2 4 USB port for memory stick Connectors for liquid sensor cables from top to bottom W Waste Rinse B Buffer B and A Buffer A Connectors for liquid container tubes Left Right ON OFF switch from left to right W Waste Rinse B Buffer B and A Buffer A Figure 2 4 Wellwash Versa side views 16 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Liquid system diagram USB memory stick port USB PC port Plate carrier Wash heads Thermo Fisher Scientific Wellwash Versa Main Parts Liquid system diagra
99. used microplate waste container priming vessel on the plate carrier disposables and all substances used in accordance with good laboratory practice GLP guidelines A Warning Inquire about appropriate collecting points and approved methods of disposal in your country state or region A If you have spilt infectious agents carry out the decontamination procedure Warning The decontamination procedure should be performed by authorized trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 e Decon 90 min 4 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A To decontaminate the instrument 7 Prepare the decontaminant Thermo Fisher Scientific Maintenance Refitting the transport lock 8 Run the Maintenance gt Cleaning Disinfection p
100. utes without liquid otherwise it may be damaged A Caution Do not touch the wash head or plate carrier when the instrument is busy A Caution Prime the liquid system with the liquid that will be used wash 34 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific Performing the operational check Settings after Thermo Fisher Scientific installation Installation Performing the operational check Before switching the instrument on Move the wash arm up and down and the plate carrier back and forth to ensure that they move freely Lower the wash arm with the wash head in place so that the tips touch the plate carrier Then move the plate carrier until the tips are aligned with the small circular indentations on the surface of the plate carrier Check that the outermost tips in the wash head are directly above the indentations to ensure that the wash arm is right angled to the plate carrier After switching the instrument on and before you put the instrument into use perform the following operational check Rotate empty bottles upside down one by one to ensure that the liquid level sensors in the bottles work properly The liquid level icon should indicate full when the bottle is upside down and empty when the bottle is in an upright position Prime the instrument to ensure that priming works and all the channels are filled with liquid For more information refer to Priming the system on page

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