Instrucciones rápidas del Fluorímetro
Contents
1. Page Setup Ss Print Page Setup Chrl P Import Export d Import Image Export Page 1 Fluerolog opi Exit 45 If you have a technical problem 1 Please consult the FluorEssence help files and this manual as well as all other manuals supplied with the sys tem If you are unable to solve the problem 2 3 52 Note the problem and any accompany ing error messages Determine FluorEssence s version number eN Data Acquisition Apaes ind Display Programming Choose the Help menu D About FluorEssence Tutorials Abat FluerEssence he About FluorEs sence win dow opens Near the bot tom are the FluorEs sence and Origin ver sion num hers About Min ipene C Click to close the window FluorEssc User s Guide v 1 0 28 Jan 2005 Write down the software s version num bers along with the purchase dates model numbers system configuration and serial numbers of the instrument and its accessories 5 Please contact a Spex Fluorescence Service Department listed below Be prepared to describe the malfunction and the attempts if any to correct it Note any error messages observed and have any relevant spectra sample polarization ratio xenon lamp scan water Raman scan etc ready for us to assist you Contact information In North America Telephone 1 732 494 8660 Fax 1 732 549 5125 World2. The data are now in an untitled project You must next create the name for the file Choose File again The File menu opens tee hapa Sew Choose Save Project sao Triada AS The Save As window appears B gt Page etg BEI Depart E lex Foe gt LAO ADS 3 19 nm AE Prope Pappi antt Egt 43 field choose Project opj from tbe list 9 Click Save Now the new project has a name To recall and open an existing project 1 Click File Ihe File menu opens 2 Choose Open C Program The Open window appears Las Date from Terrae de Pase Seta Ina Eme Pape Seta lport L brasri mags imris jane maat i prar ttiot 1 28 a ect xx a 44 FluorEssence User s Guide v 1 0 28 Jan 2005 4 Shutting Down FluorEssence 1 2 Save experiment files and data files if created In the Experiment Setup window click the Close button B Close the FluorEssence C Program FluorEssence AA Edt View Collect Graph window using either the Close button xi or in Open Samples the File drop Append down menu Exit Save Project Save Project As Ctrl N Ctri 0 D New Open Ctrl 5 Save Data As Save Template As
3. 1 04 3 28 Jan 2005 Click on the x y dataset you wish to add to the plot Choose gt to add this dataset to th plot The dataset appears in the Layer Contents column Adjust its order top to bottom with the buttons Click OK to cause the dataset to appear in the graph The Layer Number window closes 39 Merging two or more graph windows This puts all the open layers on one single page 1 40 Close all graph windows you don t want to merge In the Project Explorer at the bottom of the main Fluo resence window double click on the names of the undesired psa 062 dpa De fud guise DU JO 000545 TT I LA de Ih M MN Click the Merge button All the windows are combined onto one sheet of paper This is called a graph To preserve the old graphs while creating a new overlaid version answer Yes to the question Do vou wish to keep the old graphs A window appears asking you for num bers of rows and columns Total number of layers exactly overlay graphs choose row and column Cancel EA Number of Numer of Columns 2 e Each layer has ils own number in the upper left corner FluorEssence Uset s Gut de v LU 28 Jun 2005 Splitting two graphs by extraction This extracts each plot to separate layer in the graph 1 2 Click on the desired plot to activate it In th
4. strument and its con figuration 30 31 1 0028 Jan 2005 fluorEssence User s Louie v Click the RTC bution on the lower right The Real Time Control window opens d ABIRE a ann p m pe acest gea ce Cum a pi Al mmc dejo 18 D iniu uL Ps Wavelength in nm e F a r 34 LJ am pa Ne fers AA E I ls i roce c A li ma A This example shows the peak at 477 nm which is 10 nm too high Therefore we must calibrate the monochromator f Click the Previous Experiment button mmm The Experiment window appe h Click the Monos icon to view the monochromators index card then click the excitation monochromator tab sition of the peak in 477 mm Enter the current observe the Position Control he Click Calibrate Excitation 1 The Calibrate window opens 33 32 A Spectral Latius Calibrate Excitation 1 k In Peak of Ipferest enter the actual or expected po sition of thy peak it ought to be 467 nm Click OK At the bottom right of the Real Time Control win dow click Cancel n In the Experiment window click Run to confirm the correct peak position A correct scan is shown below i
5. In the main FluorEssence window choose the Experiment Menu button The Fluorescence Irem Main Experiment Menu appears alii M Choose Patra moimi M Spectra Mage Single Punt Phe OBS eni The Experiment Type window appears 35 FluarEssence User s Guide v 1 0 28 Jan 2005 Projects and files What is a project project js a collection of data that contains Graphs visual diagrams of the data Worksheets tables of data Notes comments about the data Datasets Axes Back grounds Graphs themselves may contain multiple kinds of information melud ing separate layers describing the data the axes the background col ors etc Concerning worksheets a dataset must contain at least two columns corresponding to x y data pairs Multiple y columns may correspond to a single x column Note For greater detail about projects graphs lay ers and how to merge combine and separate them see the Origin on line help files 37 Combining two plots datasets into one graph In its upper left SETTE corner an open graph has a small box with number in it 1 Right click on the numbered box in the upper left corner of the graph 2 Choose Layer Contents The Layer Number window opens In the Available Data column a list of x y data available to plot is shown 38 FluorEssence User s Guide v
6. time e C Program Files Jobin Yv Type from Experiment Type o A EN Ue ee m the menu press F8 while Experiment Setup say Synchronous choosing the Emission M EA The parameters oe Setup required for an prada System New Experiment emission scan are Relniti loaded into the elnitial System UI Reset i ization Experiment 4 Choose a system configuration from the list then click OK 24 10 0 FluorEssence User s Guide v 1 0 28 Jan 2005 Auto Run Pre Run previously set up experiment vious Experi without modification ment Make Overlay With an existing graph selected File creates an SPC file for use as an overlay file The existing graph should contain a single spectrum From any of these buttons upon initial start up of the soft ware you can choose a hardware configuration and experi ment type After a hardware configuration is loaded each button has its own separate function The Collect menu near the top of the main window also has these Graph Analysis Tools Fi functions plus another o important command System Ul Reset In Run Experiment case the four special my buttons are grayed out choose the System UI System New Experiment Reset command See System UI Reset Chapter 5 Technical Support for more details Click the Experiment Menu button The Select Hardware Configuration window opens e C Progr
7. Instrucciones r pidas del Fluor metro Encima del equipo no debe de haber ning n tipo de reactivo en general NADA La tapa ha de estar SIEMPRE cerrada s lo debe abrirse para cambiar las cubetas En el caj n que est bajo llave se guardan las cubetas Las cubetas se han de limpiar despu s de cada uso con agua destilada y despu s con Etanol Registrar las horas que est encendida la l mpara SIEMPRE ENCENDIDO DEL EQUIPO MUY IMPORTANTE HACERLO SIEMPRE EN ESTE ORDEN 1 pol cp Conectar el cable de alimentaci n de detr s de la torre Spectra CQ en el suelo Encender el ordenador Spectra CQ Encender el interruptor del ventilador por detr s del equipo interruptor inferior Encender la l mpara del equipo por detr s del equipo interruptor superior Esperar a que el equipo haga dos pitidos unos 2 min Encender el ordenador de sobremesa Ejecutar el programa de FluorEssence Origin Lab Hacer Click en la tecla Menu del programa M En este momento se realizar una comprobaci n del sistema IMPORTANTE entre cada paso esperar unos segundos Sino al inicial el programa la comprobaci n del equipo dar error y se tendr que reiniciar todo el sistema PARA REGISTRAR ESPECTROS DE EMISI N 1 lid e ll ra ey pa ba a y Al hacer click en la tecla Menu del programa BI Fluorescence Main Experiment Meno M nos aparecer un cuadro con varias opciones Selecc
8. Wide Web www jobinyvon com E mail info jobinyven com Technical support hours are 8 00 A M 5 00 P M Monday though Friday Eastern Standard Davylight Time In France Telephone 33 0 1 64 54 13 00 Fax 33 0 1 69 09 93 19 World Wide Web www jobinyvon fr Worldwide China 86 0 10 6849 2216 Germany 49 0 89 462317 15 Italy 39 0 2 57603050 Japan 81 0 3 58230141 UK 44 0 20 8204 8142 53
9. am FilesVobin Y Experiment Setup Advanced Setup SE Note This window does not appear if you have only one hardware configuration in stalled Skip to page 25 23 FluorEssence loads the chosen system configuration The Fluorescence Main Experiment Menu appears Fluorescence Main Experiment Menu Main Experiment Menu Spectra Kinetics 3D Anisotropy Single Point Phos Click an available scan type say Spec tra The Experiment Type window appears Choose an Experiment Type Setup window Back which appears gt For emission scan the default parameters are a water Raman scan 25 A mAs a A e 10 11 26 L IIl DEI gt IN 1 28 The Experiment Setu HT PMEAJLLLISIR LE t sell emen 1 Edi gt gt p window opens 299 mz Lnd Be A Spas ral Click the Experiment File field and en ter a new file name or select a previ ously saved file Verify that the experimental parameters are correct Be certain to check all parameters under all icons in the left hand column Insert the sample into the sample com partment and close the cover of the sample compartment Click Run is Tus v 2 Spes ds Note If the scan is ex displayed on the tremely fast the Inte
10. e toolbar choose the Extract to Layers button El E TE Sie A Note Other buttons available using the Cus tomize Toolbar command are the button for splitting each layer into a separate graph pido a and the button for merging open graph windows into one graph See the Ori on line help for more information gin 41 saving and recalling a file To save an experiment when in a new untitled experiment es To determine if you are in an untitled new experi ment examine the path shown at the top of the main FiuorEssence window I should show the word UNTI TLED at the end of the path 1 Run the experiment When the experiment is complete the Intermediate Display disappears The Project Name window appears 2 ma Project i project or browse for an existing one I Note If you are using an existing project name the soh ware will allow you to overwrite existing data or append the new data to the project 3 ClickOK The path of the project appears at the top of the main Fluor Essence window The data are now saved To save data into a new project when another project is already open 1 Run the experiment 2 Choose File The File menu opens 42 FluorEssence L sers 3 I v 1 0 28 Jan 2005 Choose New RN The New window opens ft Deer TL r 3E roger E LI Click OK
11. ionar Spectra Kinetics Aparecer n 3 opciones Emission Excitation y Synchronous Seleccionar la opci n de Emission En la ventana Experiment Setup seleccionaremos los par metros Anisotropy Single Point Phos Informaci n sobre la muestra Longitud de onda de excitaci n Rango de longitudes de onda del espectro Por ejemplo si excitamos a 500 nm el rango de longitudes de onda podr a ser de 510 a 800 nm Es aconsejable dejar un margen de unos 10 nm respecto la exc y el inicio del rango de emisi n Normalmente el valor del Slit se fija a 5 4 Una vez seleccionados los par metros hacer click en la tecla Run D Despu s de registrar el primer espectro el programa pedir el nombre del proyecto PARA REGISTRAR ESPECTROS DE EXCITACI N 1 Al hacer click en la tecla Menu programa M nos aparecer un cuadro con varias opciones Seleccionar Spectra Aparecer n 3 opciones Emission Excitation y Synchronous Seleccionar la opci n de Excitation En la ventana Experiment Setup seleccionaremos los par metros Seleccionar los par metros de manera equivalente a los de emisi n Ejemplo Emission wavelenth 550 nm si el m ximo de emisi n fuera 550nm Rango del espectro de excitaci n 350 540 nm Slits a 5 nm En este tipo de espectros es necesario seleccionar otro tipo de detector En la pestafia de la izquierda en Detectors afiadir 51 1 y eliminar el resto Comprobar que el
12. resto de variables la pesta a de Monos no han variado Hacer click en Run ICONOS B SICOS TILES Haciendo click es estos botones se puede seleccionar cualquier PRINCIPAL punto del espectro Los valores de intensidad y longitud de onda de ese punto punto se pueden visualizar en el recuadro de resultados INFORMACI N TIL SOBRE EL PROGRAMA El programa guarda los ficheros en proyectos Un proyecto contiene hojas de datos gr ficas y notas Los gr ficos pueden abrirse directamente con el programa Origin Lab sin necesidad de importar los datos Dentro del proyecto los worksheets se grabar n en la carpeta Data APAGADO DEL EQUIPO MUY IMPORTANTE HACERLO SIEMPRE EN ESTE ORDEN En general el apagado del equipo es exactamente igual que el encendido pero a la inversa 1 23 A Cerrar el programa FluorEssence Apagar el ordenador de sobremesa Apagar la l mpara del equipo por detr s del equipo interruptor superior NO APAGAR EL VENTILADOR Desconectar el ordenador Spectra CQ Desconectar el cable de alimentaci n de detr s de la torre Spectra en el suelo PASADOS AL MENOS 15 MIN DESCONECTAR EL VENTILADOR por detr s del equipo interruptor inferior Registrar el tiempo de encendido de la l mpara M s abajo se pueden ver im genes escaneadas del Manual de Instrucciones R pido MANTENIMIENTO Empresa HORIBA JOBIN YVON Persona de contacto Alberto Lozano alberto mtb e
13. rmedi Intermediate ate Display may be only in Display screen completely or rapidly dis played before the Origin window appears 1 4 FluarEssence User s Guide v 1 0 28 Jan 2005 Dine Flunaromiax General inle Ei ai eer 5 hangs Curation 1 Emnin 1 AS Dien E em You can watch the incoming data in real time along with how the positions of accessories vary The scan may paused continued or aborted all data are recorded the Intermediate Display vanishes For a new project the Project name window appears sms Project name Planta entes a project mame Enter a name for the entire project or browse for an existing project name with the Browse button then click OK All data are moved to Origin s graph window 21 AS aaa 8 Reference RT 1 2 3 3 Do post processing as needed using the Analysis menu Fi Fiuortstence C Program Files Wobin Yvon WDatalecohen WIHTITLED Elo Fak ves Collect uoh deae Monochromator parameters for the xenon lamy Signal S1 FluorEssence User s Guide v 1 0 28 Jan 2005 3 Tips amp Tricks Calibration of your instrument Excitation calibration Monochromator Initial Final Incre shis band 1200 wave w ve ment pass grooves mim length length Excitation 200 nm 600 nm nm nm Emission 350 nm 1 nm Detector parame
14. s Mantenimientos realizados Trabajo realizado Importe total XENON LAMP RECORD OF USE Time Off Total Time ETIAM TOTAL HOURS FluorEssence User s Guide v 1 0 28 Jan 2005 There are four special buttons for running experiments in b 2 Quick Guide to Running Scan 1 Turn on the host computer and all in FluorEssence struments and accessories as ex Experiment El Choose an overall type of experi ment to run such as Phosphores plained in their respective instruction cence general Spectra e g emis manuals sion or excitation or Single Point 2 Click on the FluorEssence Previous Ex Modify slightly a previously set up Ema periment experiment and run it short cut to start Fluor Essence The FluorEssence window opens ww FluorEssence C Program FilesWobin YvonWatalscohenWNTITLED ES Wew Collect Graph Analysis Tools Format Window Help salsaa al mim e r z al ls Ed Graphi OBS FluorEs Intensty Microvotts Wavelength inm Name Type size Modified _ Graph Normal 7k8 3 17 2003 19 55 3 17 2003 14 55 For Help press F1 22 VA Select Hardware Configuration Fluorolog 1 Fl Save as default Force Initialization a Immediately upon opening FluorEssence press F8 and simultaneously click the Experiment Menu button El At any
15. s at 467 nm ome e Cm m feet amo ais leje zitiely m Wavelength pt in nm i bem ma Gn deg es gig TAS Pees Cum p Tt me aem iiia Experiment Type 6 Choose Emission The water Raman experiment automatically loads mr pem gue Mie Ba dc EE Dan meae Dea imm 1 a mee ig Fa fate a T Click Run The Intermediate Display opens The water Raman scan runs 8 Ifthe water Raman scan is not at 397 nm calibrate the emission monochro mator as shown on pages 31 34 FluorEssenee Users Guide v 1 0 28 Jan 2005 Emission Calibration Monochromator parameters for the water Raman scan Monochromator Initial Final Incre Slits band 1200 wave wave ment pass grooves mm length length Excitation 350 nm 5nm Emission 365 nm 450 nm nm 5nm Detector parameters for the water Raman scan Detector Signal Integration time Units Signal S1 100 ms CPS Reference R1 100 ms mA 1 EH 0 PM E Floor sento CM rogram Files shin YeonWalalaz phun TITLED Da amp yes Gobet Guph Toole Format window telo al FIT EZ al ls El El El 9 Insert a cuvette with HPLC grade tri ple distilled water in the sample com partment Close the sample compartment s lid Start FluorEssence
16. ters for the xenon lamp scan Detector Signal Integration time Units 100 ms CPS 100 ms mA Close the sample compartment s lid Start FluorEssence In the main FluorEssence window choose the Experiment Menu button n s pinde pele The Fluorescence Main Experiment Menu appears Choose spectra The Experiment Type window ap pears FluorEssence User s Guide v 1 0 28 Jan 2005 5 Choose Experiment Type Excitation The xenon lamp scan automati cally loads oia A g P Above is an uncalibrated FluoroMax lamp scgn The main peak ought to be at 467 nm but here appears 480 nm ges Calibrate the excitation mono hromator Ferca nn Des if required a Expand the plot by clicking the Expand button D Click the cursor button to start the cursor function wm f Inort sseoce C Program f Yvon Data calibrato Drin x2 mj5e Es dee Quas isss Ioh j gwea i ajena auna 2181 Click on the graph near the peak to place the cursor on the graph d Using the left and right arrows on the keyboard move the cursor to the top of the peak 6 Read the x value of the plot this is Spectral Acquisition citatian the Note Your lamp scan wavelength may appear different 6 Click Run of the peak depending on the in The Intermediate Display opens The xenon lamp scan runs
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