Catalog No - Thermo Fisher Scientific
Contents
1. CELLULE SPeT LIGHT Reagente enzimatico per il trattamento di preparazioni di cellule prima della rilevazione mediante ibridizzazione in situ SCOPO D UTILIZZO Da usarsi per il trattamento preliminare di preparazioni di cellule prima della rilevazione mediante ibridizzazione in situ In Situ Hybridization ISH QUANTITA 40 mL REAGENTE ACCLUSO Il presente reagente enzimatico contiene pepsina ed fornito in un tampone acidico CONSERVAZIONE Conservare a 2 8 C Non congelare STABILIT Non vi sono segni ovvi ad indicazione dell instabilit di questo reagente La soluzione stata sottoposta ad un controllo della qualit per garantire la coerenza e l affidabilit della performance Non usare dopo la data di scadenza riportata sul contenitore Se si osservano le debite modalit di conservazione non vi alcuna perdita di performance significativa Qualsiasi altra condizione di conservazione del reagente che si discosti da quelle specificate deve essere convalidata dall utente PREPARAZIONE DEI REAGENTI Il reagente pronto per l uso MODALIT D USO Preparazione delle cellule Prima di eseguire il fissaggio della preparazione di cellule i vetrini dovrebbero essere trattati con un adesivo Immergere i vetrini in SSC 2x a 37 C per 60 minuti Digestione enzimatica facoltativa Eseguire la digestione enzimatica conformemente a quanto raccomandato nelle istruzioni allegate alla sonda ISH In mancanza delle is2. e invitrogen CE Catalog No 00 8402 SPeT LIGHT CELL TREATMENT REAGENT Enzyme Reagent for Cell Preparations prior to ISH Detection Lot No INTENDED USE For use in pretreating cell preparations prior to In Situ Hybridization ISH detection QUANTITY 40 mL REAGENT PROVIDED This enzyme reagent contains pepsin and is supplied in an acidic buffer STORAGE Store at 2 8 C Do not freeze STABILITY There are no obvious signs to indicate instability of this reagent It has been quality controlled to assure consistent and reliable performance Do not use after the expiration date stamped on container With proper storage there is no significant loss of performance If this reagent is stored under any conditions other than those specified those conditions must be validated by the user REAGENT PREPARATION Reagent is ready to use USAGE Cell Preparation Slides should be treated with an adhesive before mounting cell preparation Immerse slides in 2x SSC 37 C for 60 minutes Enzyme Digestion Optional Perform enzyme digestion as recommended in the instructions that come with the ISH probe The following procedure may be used if kit instructions are unavailable Cover cell preparation with at least 100 uL of enzyme reagent incubate in a humidifying chamber for 2 minutes at 37 C Two minutes is recommended for most cell preparation however longer incubation times may be required for some cell types and preparations Ov
3. erdigestion can result in lost of nuclei and chromosome structure Some cell preparations do not require enzyme digestion Authorized Representative for IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 ORF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com P1008402 Rev 10 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Cat logo N 00 8402 REACTIVO PARA TRATAMIENTO CELULAR SPeT LIGHT Reactivo enzim tico para preparados celulares antes de la detecci n mediante ISH INDICACIONES Para usar en el pretratamiento de preparados celulares antes de la detecci n mediante hibridizaci n in situ ISH CANTIDAD 40 mL REACTIVO PROVISTO Este reactivo enzim tico contiene pepsina y se proporciona en un tamp n ac dico ALMACENAMIENTO Guardar a 2 8 C No congelar ESTABILIDAD No hay signos obvios que indiquen la inestabilidad de este reactivo Se ha sometido a un control de calidad para garantizar el funcionamiento constante y fiable No utilizar una vez pasada la fecha de caducidad impresa en el envase Si se guarda adecuadamente no hay p rd
4. ida significativa del rendimiento Si este reactivo se guarda en condiciones distintas a las especificadas dichas condiciones deben ser validadas por el usuario PREPARACI N DEL REACTIVO El reactivo est listo para usar INDICACIONES Preparado celular Antes del montaje del preparado celular los portaobjetos se deben tratar con un adhesivo Sumergir los portaobjetos en 2x SSC a 37 C durante 60 minutos Digesti n enzim tica opcional Realizar la digesti n enzim tica seg n lo recomendado en las instrucciones que se proporcionan con la sonda ISH Si no se dispone de las instrucciones del equipo se puede utilizar el siguiente procedimiento Cubrir el preparado celular con como m nimo 100 uL de reactivo enzim tico incubar en una c mara humidificadora durante 2 minutos a 37 C Se recomiendan dos minutos para la mayor a de los preparados celulares sin embargo es posible que se requieran tiempos de incubaci n m s largos para ciertos tipos de c lulas y preparados La sobredigesti n puede derivar en la p rdida de n cleos y estructura cromos mica Algunos preparados celulares no requieren digesti n enzim tica Representante autorizado de IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA49RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com P1008402 Rev 10 08 DCC 08 1089 2007 Invitrogen C
5. ne berm ige Verdauung kann zu einem Verlust des Nukleus und der Chromosomstruktur f hren Einige Zellpr parationen erfordern keine Enzymverdauung Bevollmachtigter Repr sentant f r IVDD 98 79 E C Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 ORF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI008402 Rev 10 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses
6. orporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE No de catalogue 00 8402 REACTIF DE TRAITEMENT CELLULAIRE SPeT LIGHT R actif enzymatique pour pr parations cellulaires avant la d tection ISH UTILISATION PR VUE A utiliser lors du pr traitement de pr parations cellulaires avant la d tection ISH hybridation in situ QUANTIT 40 mL R ACTIF FOURNI Ce r actif enzymatique contient de la pepsine et est fourni dans une solution acide tamponn e STOCKAGE Conserver entre 2 et 8 C Ne pas congeler STABILITE Il n existe aucun signe vident indiquant une instabilit de ce r actif Sa qualit a t contr l e afin de garantir l homog n it et la fiabilit de sa performance Ne pas utiliser apr s la date de p remption estampill e sur le carton Il n y aura pas de perte de performance importante si les conditions de stockage sont ad quates Si ce r actif est conserv dans des conditions autres que celles sp cifi es il devra tre valid par l utilisateur PREPARATION DU REACTIF Le r actif est pr t l emploi USAGE Pr paration cellulaire Les lames doivent tre trait es avec un adh sif avant d y attacher la pr paration cellulaire Plonger les lames dans 2x SSC 37 C
7. pendant 60 minutes Digestion enzymatique facultatif Effectuer la digestion enzymatique tel que recommand dans le mode d emploi fourni avec la sonde ISH La proc dure suivante peut tre utilis e si le mode d emploi pour le kit n est pas disponible Couvrir la pr paration cellulaire avec au moins 100 uL de r actif enzymatique incuber dans une chambre d humidification pendant 2 minutes 37 C On recommande deux minutes pour la plupart des pr parations cellulaires Toutefois des temps d incubation plus longs peuvent tre n cessaires pour certains types de cellules et de pr parations Une surdigestion peut avoir pour r sultat une perte de structure des noyaux et des chromosomes La digestion enzymatique n est pas n cessaire pour toutes les pr parations cellulaires Repr sentant Autoris pour IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA49RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com P1008402 Rev 10 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE N catalogo 00 8402 REAGENTE PER IL TRATTAMENTO PRELIMINARE DELLE
8. tektion MENGE 40 mL MITGELIEFERTES REAGENZ Dieses Enzymreagenz enth lt Pepsin und wird in einem S urepuffer geliefert AUFBEWAHRUNG Bei 2 8 C aufbewahren Nicht einfrieren STABILIT T Es bestehen keine offensichtlichen Anzeichen f r die Instabilit t dieses Reagenzes Es wurde qualit tsgepr ft um eine gleichf rmige und zuverl ssige Leistung zu gew hrleisten Nicht nach dem auf dem Beh lter aufgedruckten Verfalldatum verwenden Bei ordnungsgem er Aufbewahrung besteht keine signifikante Leistungseinbu e Wird dieser Puffer unter anderen Bedingungen aufbewahrt als die angegebenen m ssen diese Bedingungen vom Benutzer validiert werden REAGENZVORBEREITUNG Das Reagenz ist gebrauchsfertig GEBRAUCH Zellvorbereitung Die Objekttr ger sollten vor dem Fixieren von Zellpr parationen mit Haftmittel behandelt werden Die Objekttr ger f r 60 Minuten in 2X SSC bei 37 C eintauchen Enzymverdauung Freiwilli Gem den Empfehlungen in den der ISH Probe beiliegenden Anleitungen die Enzymverdauung durchf hren Wenn die Kit Anleitungen nicht zur Verf gung stehen kann das folgende Verfahren angewendet werden Mindestens 100 uL Enzymreagenz auf die Zellpr paration geben so dass es abgedeckt ist und in einer Befeuchtungskammer 2 Minuten bei 37 C inkubieren F r die meisten Zellpr parationen werden zwei Minuten empfohlen F r einige Zelltypen und pr parationen k nnen jedoch l ngere Inkubationszeiten erforderlich sein Ei
9. truzioni per l uso del kit si pu eseguire la procedura riportata qui di seguito Ricoprire la preparazione di cellule con almeno 100 uL di reagente enzimatico incubare in una scatola di controllo dell umidit per 2 minuti a 37 C Due minuti il tempo d incubazione raccomandato per la maggior parte delle preparazioni di cellule alcune preparazioni e tipi di cellule tuttavia potrebbero richiedere dei tempi d incubazione pi lunghi La digestione eccessiva pu comportare una perdita di nuclei e della struttura cromosomica Alcune preparazioni di cellule non richiedono la digestione enzimatica Rappresentante Autorizzato per IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA49RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI008402 Rev 10 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Katalog Nr 00 8402 SPeT LIGHT REAGENZ F R DIE BEHANDLUNG VON ZELLEN Enzymreagenz f r Zellpr parationen vor der ISH Detektion VERWENDUNGSZWECK Zur Verwendung bei der Vorbehandlung von Zellpr parationen vor der In Situ Hybridization ISH De
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