Operating manual
Contents
1. HLS Operation 28 3 Operation 3 1 Functional and operator conirol elements Refer to the frontal view on the first inside cover page of this manual Fig 1 Front view of the Centrifuge 5702 Fig 2 Front view of the Centrifuge 5702 R Front of the Centrifuge 5702 RH is structurally identical to that of the 5702 R time Time dial and Start Stop button short Short run button speed Speed selector dial and rpm rcf button 6 Stand by button Open lt Lid release 1 Line socket with fuse set and main power switch device identification plate with power supply ratings rear E Emergency lid release on underside with pull cord For the Centrifuge 5702 R 5702 RH only temp Temperature selection buttons fast temp Controlled temperature button prog 1 Program button 1 prog 2 Program button 2 C Condensation water tray 3 2 Fitting and removing the rotors When fitting the rotor follow the marking triangles on each side of the rotor nut They indicate the direction of the groove on the underside of the rotor and are needed to position the rotor safely To insert the rotor rotate the driver pin of the motor shift longitudinally Fit the rotor onto the motor shaft so the marking triangles point in the same direction as the motor shaft driver pin Tighten the rotor nut by turning them clockwise using the rotor key supplied If the rotor nut does not tighten easily check whether the rotor has caug2. Centrifuge 5702 Centrifuge 5702 R Centrifuge 5702 RH eppendorf Centrifuge 5702 5702 R 5702 RH Tastenk rzel Shortcuts Display Task Lid Press ae 5702 R 0 5702 RH g Parameter change Display Display 3 8 during centrifugation E flashes flashes closed gt 2 sec 5 sec 5 sec Brake L br on br on 3 12 ON OFF br OF br OF open gt 5sec Signal L b on 3 14 ON OFF b OF open gt 2sec Parameter lock L Lo on A A 3 15 ON OFF Bi SS LLO OW open gt 2sec Program 1 set parameter prog 1 3 16 only Centrifuge 5702 R RH 7 1 2 prog 2 E gt 2 sec At set rpm L y y 3 13 L 7 gt open gt 2 sec No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2012 Eppendorf AG Hamburg Abbildung 1 Figure 1 Centrifuge 5702 Abb 1 Fig 1 2 3 speed time stand by short E open 1 Netzschalter und stecker 1 Mains switch and plug 2 Rotormutter 2 Rotor nut 3 Rotor 3 Rotor E E Notentriegelung Emergency lid release Abbildung 2 Figure 2 Centrifuge 5702 R Abb 2 Fig 2 1 2 3 E 0 temp time short Netzschalter und stecker Rotormutter Rotor Notentriegelung Kondenswasserschale nur 5702 R 1 2 3 E C speed stand by fast temp Mains switch and plug Rotor nut Rotor Emergency lid release Condensation water tray on
3. Registered trademark of Becton Dickinson Rotor code Fixed angle Angle of rotor adapter bore All Eppendorf rotors are designated and letters e g Aerosol tight version I I I according to a simple logical system which describes the technical specifi Ea 45 1 1 cations as a uniform series of numbers I I Max no tubes adapters tube adapter Swing bucket rotor bore I I Max no tubes adapters 41 006 0 040 Mode d emploi succinct 42 Mode d emploi succinct La centrifugeuse 5702 est une centrifugeuse de table non refroidie la centrifugeuse 5702 R une centrifugeuse de table refroidie et le centrifugeuse 5702 RH une centrifugeuse de table refroidie et chauffante Avant la premi re mise en service de la centrifugeuse 5702 5702 R 5702 RH il convient de lire attentivement le mode d emploi La version la plus r cente du mode d emploi et des consignes de s curit dans votre langue se trouvent sur Internet l adresse www eppendorf com Pour un aper u complet du mode d emploi nous vous recommandons de lire en outre la version en anglais pages 24 a 40 ou en allemand pages 5 a 23 plus completes Vous trouverez ce symbole sur votre centrifugeuse ainsi qu diff rents endroits de la notice d utilisation Celui ci indique la pr sence de consignes de s curit importantes N utiliser la centrifugeuse qu apr s avoir lu ces consignes de
4. C 75 max 2000 m above NSL Height 265 mm Depth 580 mm Width 380 mm 36 kg lt 25 sec lt app 25 sec lt 25 sec lt app 25 sec 2 5 A time lag 230 V 5 0 A time lag 120 V 6 0 A time lag 100 V lt 54 dB A steady state at 4 C 23 C ambient temperature empty rotor 2 39 e ep Ordering information 40 7 Ordering information Centrifuge 5702 230 50 60 Hz without rotor Centrifuge 5702 R 230 50 60 Hz without rotor Centrifuge 5702 RH 230 V 50 60 Hz without rotor Fixed angle rotor and accessories Fixed angle rotor F 35 30 17 Aluminum 30 places angle 35 for mounting of 15 ml Falcons or 15 ml round bottom centrifuge tubes complete with stainless steel tube holders for 30 places 10 places Tube holders stainless steel for rotor F 35 30 17 for mounting of 15 ml Falcons or 15 ml round bottom centrifuge tubes set of 10 Adapter conical to support 15 ml Falcons for rotor F 35 30 17 set of 10 Rubber mat for 15 ml round bottom centrifuge tubes set of 10 Fixed angle rotor F 45 24 11 Aluminum angle 45 24 places max 11 mm for mounting of 1 5 2 0 ml micro test tubes Adapter for 0 2 ml PCR tubes for rotor F 45 24 11 set of 6 Adapter for 0 4 ml micro test tubes for rotor F 45 24 11 set of 6 Adapter for 0 5 ml micro test tubes and 0 6 ml Microtainer for rotor F 45 24 11 set of 6 Fixed a
5. Debrancher la fiche secteur et attendre l arr t complet du rotor cela peut prendre 5 min Actionner le d verrouillage d urgence situ sur le c t inf rieur de l appareil en tirant sur le cordon Devant le pied ventouse avant droit se trouve dans la plaque de fond un petit bouton en plastique blanc voir E sur la figure retirer ce bouton et tirer le cordon perpendiculairement vers le bas Noter que le cordon se rentre ensuite enti rement dans le chassis Apr s l ouverture du couvercle enfoncer a nouveau le bouton en plastique dans la plaque de fond Maintenance et nettoyage Appareil Les surfaces ext rieures de la centrifugeuse et de la chambre du rotor doivent tre r guli rement nettoy es avec un d tergent Il s agit de mesures d hygiene et cela emp che la corrosion due aux contaminations En cas de contaminations dues aux produits chimiques ou solutions agressives l utilisateur est responsable d effectuer un nettoyage et une d contamination adapt s D branchez la fiche secteur avec le couvercle ouvert d vissez le rotor avec la cl fournie et nettoyez les l ments separ amp ment Nettoyez les surfaces accessibles de l appareil avec un d tergent doux Choisissez une m thode de d sinfection conforme aux directives et aux prescriptions en vigueur pour le domaine d application Utilisez par ex de l alcool thanol isopropanol ou d autres produits d sinfectants a base d alcool faut viter le liquide
6. re fois au secteur Cela vite de l endommager Enlever les s curit s de transport Au cours de la centrifugation respecter une espace libre de s curit de 30 cm autour de la centrifugeuse Ne brancher l appareil qu des sources de tension correspondant aux indications de la plaque signal tique Utiliser exclusivement des prises munies d un conducteur de protection et d un c ble secteur ad quat Avant la premi re mise en marche v rifier si le rotor est bien serr Consignes de s curit et limites d utilisation A Pour votre propre s curit il convient de respecter les dispositions suivantes La centrifugeuse 5702 5702 R 5702 RH sera exclusivement utilis e pour les applications indiqu es Elle ne peut pas tre mise en service en atmosph re explosive La centrifugation de substances explosives ou susceptibles de produire des r actions violentes n est pas autoris e En cas de passage une chambre froide a une salle de laboratoire ordinaire la centrifugeuse doit tre chauff e une demi heure dans la chambre froide ou se r chauffer pendant au moins 3 heures dans la salle du laboratoire et ne peut pas tre raccord e au r seau afin d viter tout dommage d la condensation La centrifugeuse ne peut pas tre d plac e ou heurt e pendant son fonctionnement Une centrifugeuse qui n a pas t install e correctement ou r par e par un personnel comp tent ne peut pas tre utili
7. 100 1 min or rcf start Starts the run The symbol flashes while the rotor is running stop Stops the centrifuge The 4 symbol appears briefly as soon as the rotor comes to a standstill stand by Centrifuge switched to stand by mode Open Releases the lid latch temp Changes the nominal temperature value for 5702 R 5702 RH only While the centrifuge is running the remaining time is displayed The last minute is counted down in seconds When the preset time elapses the centrifuge stops automatically and emits a signal tone to indicate the centrifugation is finished The lid of the non refrigerated Centrifuge 5702 opens automatically following centrifugation The lid of the refrigerated Centrifuge 5702 R 5702 RH remains closed to maintain the sample temperature and can be opened with the OPEN button When you switch off the centrifuge 5702 5702 R 5702 RH at the main power switch the display goes blank after a few seconds delay 3 8 Changing the centrifugation parameters during the run The timer setting the rotation speed and the temperature only 5702 R 5702 RH can be changed during the run by briefly pressing the SHORT button The display begins flashing You can then set the new centrifugation parameters with the TIME and SPEED and the TEMP dial adjustors The new centrifugation parameters are programmed after 5 seconds 3 9 Short centrifugation short Press this button with the lid closed to start a short run at
8. cord In front of the front right suction foot there is a small white plastic cap in the base plate see E in figures 1 and 2 cover flap Remove the cap and draw the cord out straight downward Please be sure to later make certain that the cord is pushed back completely into the housing before closing the lid Press the plastic button into the baseplate again 3 21 Device fuses The fuse box is located under the main power plug The fuse box can be removed from the rear The two fuses can be replaced see Ordering information 35 0 0 40 Maintenance and cleaning 36 4 Maintenance and cleaning 4 1 Device The outer surfaces of the centrifuge and the rotor chamber should be cleaned regularly with a mild cleaning agent This is for hygiene purposes and to prevent adhering impurities causing corrosion If material hazardous to health or aggressive material contaminate the device the owner is responsible for appropriate cleaning and decontamination Before cleaning unplug the power plug with the lid open unscrew the rotor using the rotor key supplied and clean it separately Use a mild cleaning agent for cleaning the accessible surfaces of the device Choose disinfection methods that correspond to the current legal regulations and guidelines for your area of application Use for example alcohol ethanol isopropanol or alcohol based disinfectants Do not allow any liquid to get into the gap at the motor shaft
9. de p n trer dans la fente la sortie de l arbre du moteur C est pourquoi la chambre du rotor doit tre uniquement nettoy e avec un chiffon humide Demandez galement au responsable de la s curit du laboratoire de vous indiquer les m thodes de nettoyage ou de d sinfections appropri es Avant d appliquer une m thode de nettoyage ou de d sinfection autre que celle recommand e par le fabricant veuillez vous assurer aupr s de la soci t Eppendorf que cette m thode ne risque d endommager ni l appareil ni les accessoires Afin de garantir un fonctionnement durable et fiable de la centrifugeuse veuillez noter que les produits chimiques agressifs peuvent endommager le rotor et la cuve Contr lez votre appareil contre les traces de corrosion et d endommagement une fois par mois Les joints en caoutchouc de la chambre du rotor doivent tre bien rinc s l eau apr s chaque nettoyage puis enduits de glyc rine ou de talc pour emp cher que les joints ne deviennent cassants Rotors L int rieur du bol du rotor les b chers herm tiques et les nacelles doivent tre nettoy s chaque mois l aide d un produit nettoyant neutre V rifier si les orifices du rotor ne pr sentent pas de traces de corrosion ou de r sidus L ensemble des rotors des b chers herm tiques des adaptateurs et des nacelles sont con us pour r sister l autoclave 121 C pendant 20 min Les capuchons tanches aux a rosols y compris le joint en si
10. des crous pose probl me v rifier que le rotor s est bien enfonc sur le tenon d entra nement de l arbre du moteur Charger le rotor de fa on sym trique et fermer le couvercle de la centrifugeuse Eviter une charge non quilibr e du rotor Apr s la centrifugation le couvercle de la centrifugeuse 5702 s ouvre automatiquement le couvercle de la 43 puns 10 dW93 p IPON Mode d emploi succinct 44 Mode d emploi succinct centrifugeuse r frig r e 5702 R et 5702 RH reste ferm pour maintenir la temp rature de l chantillon et peut tre ouvert via la touche OPEN time Permet de modifier la dur e de fonctionnement Le positionnement du bouton tournant TIME au dela de 99 min ou en dega de 0 5 min g n re la fonction de marche en continu La marche en continu est indiqu e par le message oo sur l affichage du temps speed Permet de modifier la vitesse par incr ments de 100 1 min ou en acc l ration x g La commutation entre les incr ments de 100 1 min et l acc l ration x g s effectue en appuyant sur la touche SPEED start Permet le d marrage de la centrifugation Le symbole I clignote et indique que le rotor tourne stop u Arr te la centrifugation Le symbole W appara t des que le rotor s arr te et que le couvercle de la centrifugation peut tre ouvert stand by Permet de mettre en marche et d arr ter la centrifugation Open Permet de d verrouiller le couvercle temp modifie la valeur th or
11. for at least 5 seconds with the centrifuge lid open After 5 seconds the dial adjuster is disabled and the centrifugation parameters are locked against unintentional adjustment Lo on appears in the display of the Centrifuge 5702 In the display of the Centrifuge 5702 R 5702 RH the locked parameters are symbolized by an additional pictogram The preset parameters can then no longer be altered whether the centrifuge is running or not The START STOP button can still be used to start and stop the centrifuge however It is also still possible to switch the signal tone and rcf switch on and off With the Centrifuge 5702 R 5702 RH it is also possible to additionally lock a program called up against unintentional resetting A program already written is called up through the program buttons and then as described above the program buttons and dial adjustor setting deactivated To enable the dial adjuster again press and hold down OPEN and SHORT button simultaneously for 5 seconds with the centrifuge lid open The display shows Lo OF or w 5702 R 5702 RH The current status Lo on can be displayed on the Centrifuge 5702 by briefly pressing the SHORT and OPEN buttons while the lid is open 33 0 40 Operation 34 3 Operation 3 16 Programming for 5702 R 5702 RH only With the Centrifuge 5702 R 5702 RH it is possible to store up to two permanent programs only during standstill First set the required progra
12. glass breakage carefully remove all splinters and all ground glass from the rotor the buckets the adapters and the rotor chamber You may need to replace the rubber mats and adapters to prevent further damage Fine splinters of glass may otherwise scratch the surface of the rotors and buckets reducing their resistance to chemicals The air turbulences within the rotor chamber produce a very fine black powder of abraded metal In addition to causing damage to the rotor chamber rotor buckets and adapters the powder also contaminates the samples Check the rotor bores regularly for residues and damage 4 5 Returning devices When returning centrifuges please ensure that these devices are fully decontaminated so that they do not present a health risk to our service staff You will find additional information and a blank of the decontamination confirmation at www eppendorf com Do also consult your laboratory safety officer about a suitable decontamination method Please fill out the decontamination confirmation and place it together with the device when it is to be sent back to Eppendorf 37 Buiuesp pue Troubleshooting 38 5 Troubleshooting Error Cause Remedy Stand by button lit red Centrifuge not ready Press STANDBY button No display No power Power failure Plug power cable in on both ends Check power fusing of lab device Lid will not
13. m thode de d contamination appropri e veuillez vous adresser galement au responsable de la s curit de votre laboratoire Veuillez remplir l attestation de d contamination et joignez la compl t e a l appareil lorsque celui ci doit tre renvoy la soci t Eppendorf 45 puns 10 dW93 p IPON Mode d emploi succinct Mode d emploi succinct Tableau d identification des anomalies Anomalie Origine Remede Aucun affichage Absence d alimentation Coupure secteur V rifier le raccordement secteur des deux c t s Contr ler les fusibles sur le tableau d alimentation du laboratoire sur l appareil Couvercle impossible ouvrir Coupure secteur Le rotor tourne encore D verrouillage d urgence Attendre l arr t complet du rotor Vibration de la centri fugeuse au d marrage Le rotor est charg de fa on asym trique Arr ter la centrifugeuse et r tablir la sym trie de charge D marrage impossible de la centrifugeuse Couvercle non ferm Pousser fermement sur le couvercle Affichage LID Couvercle non verrouill Couvercle non verrouill Pousser fermement sur le couvercle actionner Start Stop ou le d verrouillage d urgence Fermer nouveau le couvercle Lo on Blocage des param tres de Le couvercle de la centrifugeuse tant ouvert appuyer uniquement 5702 centrifugation contr
14. maximum speed The centrifuge stops when you release the SHORT button again 31 0 0 40 Operation 32 3 Operation 3 10 Continuous running time The continuous run function is set by turning the TIME dial to either above 99 minutes or below 0 5 minutes The timer displays oo to indicate that continuous running is active The time is counted upward in minutes stop Ends continuous running 3 11 Ref display and calculation SPEED Press the dial to toggle the display between 1 min rpm and rcf and vice versa Note that the rcf g force indicated when the display is switched is standardized to 15 ml Falcon tubes in the rotor A 4 38 At 4 400 1 min you can achieve the following maximum rcf with the various adapters Rotor Adapter Max centrifuging Max rcf g force radius cm rmax rounded A 4 38 1 5 2 0 ml 13 4 2 900 Round bucket 1 1 1 4m 13 2 2 850 2 7ml 13 2 2 850 2 6 7ml 13 2 2 850 4 10ml 13 2 2 850 9 15ml 13 0 2 800 15 ml Falcon 13 7 3 000 25 ml 13 5 2 900 50 ml Falcon 13 5 2 900 85 ml 13 5 2 900 A 4 38 5 7 ml 12 7 2 750 Rectangular bucket 9 ml 127 2 750 15 ml 12 7 2 750 20 ml 12 7 2 750 25 ml 12 7 2 750 A 8 17 15 ml Falcon 12 8 2 770 15 ml DIN tube 12 8 2 770 F 35 30 17 15 ml 12 7 2 750 Outer ring 15 ml Falcon 12 7 2 750 F 35 30 17 15 ml 10 7 2 300 Inner Ring 15 ml Falcon 10 7 2 300 F
15. open Power failure Rotor still running Emergency lid release see section 3 20 Wait for rotor to stop Centrifuge shakes when starting up Rotor unevenly loaded Stop centrifuge and load evenly Centrifuge will not start Lid not closed Press lid down or press OPEN button Display LID Lid not released Press lid down tight press Start Stop or emergency lid release Lid not locked Close lid again Lo on Centrifugation parameters With open centrifuge lid simultaneously press SHORT only 5702 locked against adjustment and OPEN for 5 seconds see 3 15 if parameters should be readjusted P1 Program 1 or 2 is called up Press illuminated program button to exit the program P2 level if required FA Display indicates a FAST TEMP The run can be interrupted with the START STOP run button if required Inb Rotor unevenly Check loading and repeat run loaded Int Power failure during run Check power plug Wait for rotor to come to a stop Repeat run Er2 Unbalanced rotor switch Inform Service defective Er3 Error in speed system Leave device switched on until error message disappears up to 5 min Er5 Lid switch Close lid restart Er 6 Drive error Repeat run Er 7 Overspeed Error in drive or speed measurement system Er 8 Drive error Repeat run Er9 25 Electronics error Repeat run Er 18 Temperature deviation Nominal value setting too low or refriger
16. outlet For this reason the rotor chamber should be cleaned only with a damp cloth You should also consult your laboratory safety officer with regard to a suitable method of cleaning and disinfecting Before any cleaning or disinfecting method other than that recommended by the manufacturer is used please check with Eppendorf that the intended method will not damage the device or its accessories In order to ensure long term reliable work with your centrifuge please note that aggressive chemicals may damage the rotor and the chamber Check your device once a month for corrosion and damage The rubber seals in the rotor chamber should be rinsed off thoroughly with water and lubricated with glycerin or talc after every clean to prevent them becoming brittle 4 2 Rotors The rotors buckets tube holders and adapters should be cleaned once a month or when necessary with a neutral cleaning agent to prevent residues of the material being centrifuged from changing the properties of the centrifuge and its accessories The rotor must be taken off for this Never place components in disinfectants or cleaning agents which contain sodium hypochlorite chlorine or are oxidants This will cause the material to change Disinfection with glutaraldehyde solution is possible We recommend Cidex activated glutaraldehyde solution The plastic adapters and rubber plates are dishwasher safe Check the tube holders and buckets for residues and corrosion For thoro
17. s curit Utilisation correcte La Centrifuge 5702 5702 R 5702 RH est destin e uniquement tre utilis e l int rieur et a t con ue pour s parer des fluides et des l ments en suspension de densit s diff rentes dans des microtubes agr s Cet appareil doit amp tre uniquement utilis par le personnel de laboratoire ayant regu la formation n cessaire L utilisateur doit avoir pris soigneusement connaissance du manuel d utilisation et s tre familiaris avec le fonctionnement de l appareil Installation de l appareil A L appareil est lourd Soulever et porter l appareil peut entra ner des probl mes de dos gt Transportez l appareil uniquement dans son emballage d origine gt Transportez l appareil a l aide d une deuxi me personne gt Pour les longues distances pr voyez un quipement de transport par ex l ve charges Placer la centrifugeuse sur une table de laboratoire stable horizontale et exempte de r sonance S assurer d une bonne ventilation de l environnement de la centrifugeuse et vider toute incidence solaire directe Afin d assurer une bonne ventilation de l appareil il est n cessaire de respecter un cart de 15 cm entre l appareil et les appareils voisins ainsi qu l arri re de l appareil Ceci est absolument n cessaire pour la puissance frigorifique de la 5702 R 5702 RH La centrifugeuse doit tre pr chauff e 3 heures dans le laboratoire avant de la raccorder pour la premi
18. someone else please include the instruction manual Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC on waste electrical and electronic equipment WEEE According to these regulations any devices supplied after 13 08 05 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations within the EU may vary from country to country please contact your supplier if necessary Si Declaration concerning the ATEX directive 94 9 EC The present design and ambient conditions inside Eppendorf centrifuges mean that they are not suitable for use ina potentially explosive atmosphere The centrifuges must therefore only be used in a safe environment such as the open environment of a ventilated laboratory or a fully extracted fume hood The use of substances which may contribute to a potentially explosive atmosphere is not permitted The final decision on the risks in connection with the use of such substances is the responsibility of the user of the centrifuge 27 N 0 euoyesipdde pue 0
19. 11 37 Returning devices 7 111 03 37 Troubleshooting guide ss asana aana eee ee eee eee eee aaa na eee 38 Technical data 71 1 5 5 39 Ordering information 2 22 03 2 2206 zu sun 333 a a NG dan data a aaa aa 40 1 Introduction Centrifuge 5702 is non refrigerated Centrifuge 5702 R is refrigerated and Centrifuge 5702 RH is a refrigerated and heatable bench top centrifuge For these centrifuges following rotors are available Fixed angle rotor F 35 30 17 with a capacity of 30 x 15 ml centrifuge tubes Fixed angle rotor F 45 24 11 with a max capacity of 24 x 1 5 2 0 ml in micro test tubes Fixed angle rotor F 45 18 17 Cryo with a max capacity of 18 x cryotubes max 17 mm Swing bucket rotor A 4 38 with a max capacity of 4 x 85 ml in round buckets and 4 x 90 ml in rectangular buckets Swing bucket rotor A 8 17 with a max capacity of 8 x 15 ml Before using the centrifuge 5702 5702 R 5702 RH for the first time please read the operating manual The latest version of the manual and the safety instructions in your language can be found on the Internet at www eppendorf com You will see this symbol on your centrifuge and at a number of points throughout this manual The texts it highlights are relevant to safety Use the centrifuge only after having read the safety notices 1 1 I
20. 45 24 11 0 2 ml 6 6 1 430 0 4 ml 8 2 1 770 0 5 0 6 ml 7 4 1 600 1 5 2 0 ml 8 2 1 770 F 45 18 17 Cryo without adapter 9 1 1 970 with adapter 8 9 1 930 To ascertain the maximum rcf for a specific adapter you can apply the following formula as per DIN 58 970 ref 1 118 10 5 n2 rmax n rotational speed in 1 min max Max centrifuging radius in cm Example The 85 ml adapter has a maximum radius of 13 5 cm At 4 000 rpm a maximum ref of 2 415 x g is reached 3 Operation 3 12 Adjusting the acceleration and braking ramps When working with Ficoll density gradients or other sensitive applications e g certain types of blood and urine centrifugations slower acceleration and braking ramps can be switched on for the Centrifuge 5702 5702 R 5702 RH This makes the centrifuge accelerate and decelerate gently This guarantees optimal centrifugation results Press the SHORT key for longer than 5 seconds while the centrifuge lid is open On the Centrifuge 5702 the text in the display changes from br on brake on to br OF brake off The symbol appears in the display of Centrifuges 5702 R RH The slower acceleration and braking ramps are now activated The current status will be displayed when the SHORT button is pressed for less than 5 seconds while the centrifuge lid is open To switch faster acceleration and braking back on press the short key for longer than 5 seconds again while the centrifuge lid is open The b
21. C By way of preprogrammed temperature profiles it is possible to set optimized temperature arcs for the available swing bucket and fixed angle rotors This enables exact temperature control even for sensitive samples Setting the preprogrammed temperature profiles Pressing the Fast Temp key while the centrifuge lid is open results in the display of the set rotor with the corresponding temperature profile Using the arrow keys it is possible to select other temperature profiles The displays appearing stand respectively for the following rotors Display Rotor ro F 35 Fixed angle rotor F 35 30 17 ro F 24 Fixed angle rotor F 45 24 11 roF 18 Fixed angle rotor F 45 18 17 Cryo ro A4 rE Swing bucket rotor A 4 38 with rectangular buckets ro A4 ro Swing bucket rotor A 4 38 with round buckets ro A8 Swing bucket rotor A 8 17 ro ALL General temperature profile for all rotors After 5 seconds a selected temperature profile will be automatically adopted and the display will return to the operating status temp The temperature nominal value can be set using the arrow keys During the rest period of the centrifuge the current temperature and the temperature nominal value are displayed alternately current temperature short temperature nominal value long Only the current temperature is displayed during the run A deviation of the set temperature nominal value of more than 3 C is signalled during the run by a blinking temperature display fast
22. arameters during the run 31 SMOMCENUMMUGATION Au an ae MARIA nee date 31 Continuous ec 32 Ret display and calculation sire ren 22 ar ana ar da rn 32 Adjusting the acceleration and braking ramps 33 AUSCUIDM 222 tet atte sr re dete wad eae aaa eee ee ee a ected Y 33 Activating and deactivating the signal tone 0 0 0 eect tenes 33 Parameter loCk sakan aa aas Een Sad pea bio 33 Programming for 5702 R 5702 RH only 4eeee oe 34 Program selection for 5702 R 5702 RH only 2eeeeee es 34 Refrigeration for 5702 R only 0 ccc eet 34 Heating and refrigeration only for 5702 RH 0 eee ttt 35 Opening the centrifuge in case of power failure 35 DevicefUses sh ott sider tye adit ee ee 35 Maintenance and cleaning 00 cee eee ee eee eee eee 36 DEVICE nie 11 1 77 7 bd ibs 36 MM 7 7 77 1 1 na Un a 36 Refrigerated centrifuges 92 7 1 36 Glass breakages 7 77 7 7 1 1
23. ation defective gt 5 C fr nominal value Er 19 Refrigeration unit overheated Make sure that the air circulation through the cooling slots is not impaired Er 23 Motor overheating Allow motor to cool only 5702 Er 24 Fault in the refrigeration unit At start of operation contact Service After longer running time allow centrifuge to cool down If the suggested remedy repeatedly fails please contact Service 6 Technical data Line connection Power output Max speed Max centrifugal acceleration Max load Max kinetic energy Permissible density of material being centrifuged Ambient temperature Max rel air humidity Positioning height Dimensions Weight excluding rotor Startup time 230 V Deceleration time Startup time 100 120 V Deceleration time Fuses Noise level Overvoltage category Degree of contamination Technical specifications subject to change Centrifuge 5702 230 V 50 60 Hz 120 V 50 60 Hz 100 V 50 60 Hz 200 W 4 400 rpm 3 000 rcf 4 x 90 ml 2 280 Nm 1 2 g ml 2 40 C 75 max 2000 m above NSL Height 241 mm Depth 395 mm Width 320 mm 20 kg lt 25 sec lt 25 sec lt 25 sec lt 25 sec 2 5 A time lag 230 V 5 0 A time lag 120 V 100 lt 58 dB A Centrifuge 5702 R 5702 RH 230 V 50 60 Hz 120 V 50 60 Hz 100 V 50 60 Hz 380 W 4 400 rpm 3 000 rcf 4 x 90 ml 2 280 Nm 1 2 g ml 10 40
24. bezeichnung Product name ce ce 3 ce u c amp ri r 6 C ce ce ce Ce ce i ce CE ce ce 6 ce 6 ce ce C ce ce ce ce ce ce ce ce CE 6 A A A ec eve ce mr ce y rn ce ey re ce Se ce er A ce ce C ce ce or ce ce 6 c rr ce ce vy ce Se CE ee 4 ce Le c 6 ce ce rk 61326 Te ce 6 6 987 ce cee C 9 EG EN ISO 14971 E 010 2 101 EN 61326 2 6 EN 62366 O 18113 3 ce ce ce 6 3 ce ck C d A ce C 7 ce C ce C ce ce CE 6 ce 6 ce ce Te Irre lt ec 6 x or Vorstand Board of Management Pre eae A Ce ee CE c ce Hamburg Date 6 ee E eC 6 Ge 7 c c ce ce ce ce ce ce ce ce te ce Le cl ce rr rr 6 ce ce ce Ce ce C ce ce ce ort cf ce c eS ce CONG CENG CE Te CE TE CE cE SS CES Se in ae GS ce cE Te CE Ce ce CE ce CE ce CE ce CE Ce ce Se CS Ye CS Ce Ce M Ce Se Ce CE Le Ce CY CE disons CE Te CE Ta CE Te CE ce CP ce CY ce Se CE Ye CE Te oob rett Ce C CERTIFICATE OF COMPLIANCE Certificate Number Report Reference Issue Date Issued to This is to certify that representative samples of Standard s for Safety Additional Information 20120416 E215059 E215059 2002 February 28 2012 April 16 EPPENDORF ZENTRIFUGEN GMBH RIESAER STR 198 04319 LEIPZIG GERMANY Laboratory use Electrical Equipment Centrifuge Model 5702 Have been investi
25. e tout simultan ment sur SHORT et OPEN pendant 5 s si d r glement vous souhaitez modifier les param tres P1 Le programme 1 ou 2 est activ Appuyer sur la touche allum e du programme afin de P2 quitter le niveau du programme si souhait FA Indique une temporisation FAST Si n cessaire la temporisation peut tre interrompue TEMP via les touches START STOP Inb Charge irr guli re du rotor Contr ler la charge et renouveler la centrifugation Int Coupure secteur durant le Contr ler le raccordement secteur Attendre l arr t du fonctionnement rotor Refaire un essai Er 2 tecteur de balourd d fectueux Prendre contact avec le SAV Er 3 Syst me compte tours Laisser reposer l appareil sous tension jusqu ce que le d fectueux message d erreur s efface jusqu 5 min Er 5 Commutateur du couvercle Fermer nouveau le couvercle Er 6 Entra nement d fectueux Refaire un essai ga Erreur dans le syst me d entra nement ou celui du Er7 Surr gime compte tours Er 8 Erreur d entra nement Renouveler la centrifugation Er 9 25 Probl amp me lectronique Refaire un essai Er 18 Ecart de temp rature gt 5 C par Valeur th orique r gl e trop faible ou refroidissement rapport a la valeur th orique d fectueux Er 19 Surchauffe du circuit de V rifier que la circulation de l air travers les fentes de refroidissement refroidissement n est pas entrav e distance lat rale minimale 15 cm Er 23 Surchauffe du moteur Lais
26. ected against direct sunlight To ensure that the ventilation of the device is not impaired a fundamental clearance of 30 cm to the side anda min of 15 cm to the back wall must be maintained This is especially important for the refrigeration capacity of the 5702 R RH During centrifugation according to the recommendations set out in EN 61010 2 020 a safety clearance of 30 cm must be maintained around the centrifuge within which there are no objects which may be destroyed and so cause further damage Only connect the device to power supplies which correspond with the electrical requirements on the name plate Only use sockets with a protective earth PE conductor and suitable power cable Now connect the centrifuge to the power supply and switch it on at the main power switch on the rear side see cover flap The centrifuge is now ready for operation The Stand by button lights up green and the display is active Before starting check that the rotor is firmly seated 25 0 0 1 Safety precautions and applicational limitations 26 2 Safety precautions and applicational limitations AN For your personal safety please be sure to comply with the following regulations unconditionally The centrifuge 5702 5702 R 5702 RH must only be use for the specified applications see Introduction It must not be operated in explosive atmospheres Explosive or highly reactive substances must not be ce
27. ened For mechanical stability all the places on the rotor must be fitted with identical buckets The rotor may only be loaded symmetrically Opposing tubes should be of the same type and be filled equally On the rotor you will find information concerning the weight that a completely filled bucket may not exceed Prior to centrifugation the tubes should in any case be visually inspected for material damage Damaged tubes may not be centrifuged This is because broken tubes can in addition to sample loss result in further damage to the centrifuge and accessories Rotors showing clear signs of corrosion or mechanical damage must not be used Check the accessories regularly Rotors are high grade components which have to withstand extreme stresses and strains Aluminum rotors are largely protected from corrosion by the most common laboratory chemicals by means of an anodized coating though the protection is not unlimited Protect the rotors from mechanical damage Even minor scratches or cracks can result in serious internal material damage Avoid damaging the rotors by the use of aggressive chemicals such as strong and weak alkalis strong acids solutions of mercury copper and other heavy metal ions chlorinated hydrocarbons concentrated salt solutions and phenol If the rotor is contaminated by aggressive substances clean it immediately with a mild cleaning agent The material being centrifuged may not exceed a density of 1 2 g ml at maximu
28. ertificate Number Report Reference Issue Date Issued to This is to certify that representative samples of Standard s for Safety Additional Information 20120416 E215059 E215059 2002 August 27 2012 April 16 EPPENDORF ZENTRIFUGEN GMBH RIESAER STR 198 04319 LEIPZIG GERMANY Laboratory use Electrical Equipment Centrifuge Model 5702R 5703 and 5702RH 5704 Have been investigated by UL in accordance with the Standard s indicated on this Certificate ANSI UL 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and C22 2 No 1010 1 1992 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part General Requirements Part 2 Particular Requirements for Laboratory Centrifuges CAN CSA C22 2 No 1010 2 020 92 CAN CSA C22 2 No 1010 2 020A 97 See the UL Online Certifications Directory at www ul com database for additional information Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by UL s Listing and Follow Up Service meeting the appropriate requirements for US and Canada The UL Listing Mark for the US and Canada generally includes the UL in a circle symbol with C and US identifiers the word LISTED a control number m ay be alphanumeric assigned by UL and the product category name product identifier as indicated in the appro
29. gated by UL in accordance with the Standard s indicated on this Certificate ANSI UL 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and C22 2 No 1010 1 1992 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part General Requirements Part 2 Particular Requirements for Laboratory Centrifuges CAN CSA C22 2 No 1010 2 020 92 CAN CSA C22 2 No 1010 2 020A 97 See the UL Online Certifications Directory at www ul com database for additional information Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by UL s Listing and Follow Up Service meeting the appropriate requirements for US and Canada The UL Listing Mark for the US and Canada generally includes the UL in a circle symbol with C and US identifiers A the word LISTED a control number may be alphanumeric assigned by UL and the product category name product identifier as indicated in the appropriate UL Directory Look for the UL Listing Mark on the product William R Carney Director North American Certification Programs UL LLC Any information and documentation involving UL Mark services ae provided on behalf of UL LLC UL or amy authorized licenses of UL For questions please contact local UL Customer Service Representative at www ul comicontactus Page 1 of 1 CERTIFICATE OF COMPLIANCE C
30. ht on the motor shaft driver pin To release the rotor turn the rotor nut anticlockwise using the rotor key 3 3 Loading the rotors The rotors and buckets round and rectangular must be loaded symmetrically The adapters must be loaded only with the specified tubes Minimize the difference in weight between the filled sample tubes This will reduce wear on the drive and cut running noise The centrifuges 5702 5702 R 5702 RH have an automatic imbalance detector which shuts down the centrifuge if the weight differences are excessive This is indicated by the error message Inb in the display A The maximum weight for a fully loaded bucket is indicated on each rotor 3 Operation 3 4 Fixed angle rotor The fixed angle rotor F 35 30 17 can be loaded with 15 ml Falcons and 15 ml round bottom centrifuge tubes It is available in the 10 and 30 position versions Please use only swing bucket rotor A 4 38 with round buckets and the appropriate adapters for centrifuging blood sampling systems Before centrifuging the Falcons place their plastic adapters in the tube holders The adapters prevent the Falcons from being destroyed For the centrifugation of round bottom tubes made of Duran glass round rubber pads are required to prevent the tubes from cracking Make sure a rubber pad is placed under each glass tube before centrifuging In order to avoid breakage of the tubes when using centrifuge tubes of glass polystyrene or similar materia
31. ique de la temp rature uniquement pour la 5702 R 5702 RH La valeur th orique de la temp rature peut tre r gl e au moyen des touches fl ch es de 9 C 40 C 9 C a 42 C pour la centrifuge 5702 RH Au cours de l arr t l cran indique alternativement la valeur th orique r gl e de la temp rature et la temp rature actuelle dans la chaudi re du rotor Au cours de la centrifugation la temp rature actuelle dans la chaudi re du rotor est la seule tre indiqu e fast temp d marre une temporisation avec un r gime sp cifique a la centrifugation un message d cran FA La temporisation se termine automatiquement ou en appuyant sur la touche STOP Un signal sonore p riodique retentit Le temps la temp rature et la vitesse de rotation peuvent tre modifi s en cours de centrifugation Pour ce faire actionner au pr alable la touche SHORT ce qui entra ne le clignotement de l affichage de centrifugeuse Apr s modification des parametres de centrifugation la nouvelle valeur de consigne pour la vitesse de rotation est appliqu e Le temps de fonctionnement restant est affich durant la centrifugation En cas de modification du temps en cours de centrifugation le temps d j coul est pris en compte La derni re minute est d compt e en secondes Lorsque le temps r gl s est coul la centrifugeuse s arr te automatiquement short Le fait dappuyer sur cette touche lorsque le couvercle est fer
32. licone sont soumis l usure naturelle et doivent tre remplac s en cas de d gradation perceptible Une attention particuli re devra tre accord e aux rotors libres en veillant la stricte propret des pivots et des rainures recevant les b chers herm tiques et les nacelles Ils devront tre lubrifi s l aide de la graisse fournie de sorte que les b chers puissent osciller librement Les b chers herm tiques ne doivent pas tre stock s avec des capuchons serr s Verre cass Lors de la centrifugation de tubes en verre il faut savoir que le risque de bris de verre s accro t au fur et mesure que la vitesse de rotation ou de centrifugation augmente Veuillez respecter les indications du fabricant relatives la charge maximale des tubes de centrifugation En cas de bris de verre veuillez retirer imm diatement tous les clats et le verre pulv ris du rotor des tubes des adaptateurs et de l espace de la centrifugeuse Eventuellement les plaques en caoutchouc et les adaptateurs doivent tre chang s pour viter davantage de d g ts Renvoi des appareils Lors du renvoi de centrifugeuses veuillez prendre garde ce que les appareils soient enti rement d contamin s et qu ainsi ils ne pr sentent aucun risque pour la sant de notre personnel d entretien Vous trouverez d autres informations et un mod le de l attestation de d contamination sous www eppendorf com Pour obtenir des renseignements propos de la
33. ls please observe the limiting data with reference to endurance specified by the manufacturer The maximum load capacity for Falcons is 20 x 15 ml for 15 ml round bottom centrifuge tubes 30 x 15 ml The rotor can be loaded with a combination of 15 ml Falcons and 15 ml round bottom centrifuge tubes The maximum capacity is then 20 x 15 ml Falcons and 10 x 15 ml round bottom centrifuge tubes 15 ml round bottom centrifuge tube S IK NO oy I 15 ml Falcon ZEN KZ Fig 3 Loading of rotor F 35 30 17 with 15 ml Falcons and 15 ml round bottom centrifuge tubes The specified max weight of 56 g imprinted on the rotor is the gross weight rating including the tube holder adapter tubes and contents Always weigh the tube holder and the load together A maximum of 24 1 5 2 0 ml test tubes can be centrifuged in rotor F 45 24 11 If the appropriate adapters are used it is also possible to load it with 0 2 ml PCR tubes 0 4 ml test tubes 0 5 ml test tubes and 0 6 ml Microtainers Fixed angle rotor F 45 18 17 Cryo can be equipped with cryotubes max diameter 17 mm or 13 mm with adapters and sealable centrifuge tubes max diameter 16 5 mm or 12 2 mm with adapters In each of these cases max tube length is 50 mm Maximum load adapter tube and contents per bore is 3 75 g on rotor F 45 24 11 and 8 7 g on rotor F 45 18 17 Cryo When loading the rotors please make sure that the tube
34. ly 5702 R Front Centrifuge 5702 RH baugleich mit Centrifuge 5702 R Front of the Centrifuge 5702 RH is structurally identical to that of the 5702 R Contents 24 Introduction er a een ere ee es a re 25 Intended se ake erie 25 Delivery PACKAGE 250 aaa BR A ana Ba san 25 Installing the device 3 dened nd OS Edd Bg aaa mont D dote de bbe ed 25 Safety precautions and applicational limitations 26 Operation 2 eee 1 0 0 28 Functional and operator control elements 2 28 Fitting and removing the rotors lt aaa rraren arena 28 LOAdINGANG fotos 22224420 1 0 28 Fix d anglerotOrs 3 1 0 1311 29 Swing bucket rotors 00 6 30 Operation of round bucket with aerosol tight caps tte 31 Centrifugation with timer setting 0 0 0 cece tenet eee 31 Changing the centrifugation p
35. m entraine une centrifugation br ve a r gime maximal La centrifugation est arr t e en relachant la touche SHORT Programmation Dans la centrifugeuse 5702 R 5702 RH il est possible d enregistrer jusqu deux programmes fixes uniquement possible l arr t R gler pour cela les param tres souhait s Maintenir appuy es PROG 1 ou 2 jusqu ce que la touche de programmation ne clignote plus et soit allum e en permanence Le programme est enregistr Un appui bref sur la touche de programmation entra ne l appel du programme souhait Arr t de la fonction de freinage Pour un arr t progressif sans freinage appuyer sur la touche SHORT pendant plus de 5 secondes le couvercle de la centrifugeuse tant ouvert L inscription l cran passe de br on frein activ br OF frein inactiv ou soft s affiche 5702 R 5702 RH le frein de la centrifugeuse est maintenant inactive R glage fixe des param tres Contre un d r glement involontaire le couvercle de la centrifugeuse tant ouvert appuyer simultan ment sur les touches SHORT et OPEN pendant 5 secondes De cette mani re les param tres ou les programmes d finis de mani re fixe ne peuvent plus tre d r gl s aussi bien lors de la centrifugation qu l arr t une centrifugation peut quand m me tre d marr e ou arr t e via les touches START STOP Mode d emploi succinct Ouverture de la centrifugeuse en cas de coupure de courant
36. m data with the TIME and SPEED dial adjustors The function at set rom and the switched off braking function can also be stored if necessary in the permanent program PROG 1 or 2 Hold down for 2 seconds until the program button is no longer flashing and lights up continuously A brief signal will sound The program is now stored 3 17 Program selection for 5702 R only Program selection is only possible during stillstand PROG 1 or 2 Pressing once calls up the required program The button for the program activated lights up in blue If dial adjustors or buttons are required for the program setting the program remains unchanged The text Pr 1 Program 1 or Pr 2 Program 2 appears in the centrifuge display Exit the program by again pressing the program button The blue lighting of the button extinguishes and the centrifugation parameters can be set freely again 3 18 Refrigeration for 5702 R only temp The nominal temperature value can be set with the arrow keys from 9 C to 40 C During stillstand the nominal temperature setting and the current temperature in the rotor chamber are displayed alternately With the centrifuge running only the current temperature in the rotor chamber is displayed A deviation of more than 3 C from the temperature set is signalled during the run fast temp starts a controlled temperature run with a centrifuge specific rotation speed in order to quickly reach the selected tempe
37. m rotation speed If the rotor is run for a lengthy period of time or more often with short centrifugation runs the sample tubes will become hot Keep within the limits specified by the tube manufacturers Seal the tube lid down tight before centrifuging The lids of unclosed tubes may rip off during centrifugation and damage the centrifuge When using organic solvents e g phenol chloroform the durability of plastic tubes may be impaired When closing the centrifuge lid do not place your fingers between the lid and the centrifuge otherwise they may be trapped 2 Safety precautions and applicational limitations The following rotors and accessory buckets have a maximum service life of 7 years A 4 38 5702 720 003 and 5702 740 004 A 8 17 5702 700 002 The date of manufacture is engraved on them in the format 10 01 October 2001 Transparent aerosol tight caps made of polycarbonate have a maximum service life of 3 years The date of manufacture is embossed on them in the form of a clock RS Aerosol tight caps may lose their sealing strength when exposed to organic solvents e g phenol chloroform Check the aerosol tight caps regularly for changes or cracking Aerosol tight caps showing cracks or other changes must be replaced immediately Rotors caps or buckets which have been damaged by chemical or mechanical factors or which have passed maximum service life may no longer be used Transfer If the device is passed on to
38. n 30 x 115 2 adapters 1 x 85 ml 38 x 106 Rectangular bucket 90 ml for A 4 38 set of 4 Rectangular bucket 90 ml for A 4 38 set of 2 Do not use with aerosol tight caps Adapter for standard tubes for rectangular buckets 2 adapters 10x 5 7ml 2 adapters 8x 9ml 2 adapters 6x 15 ml 2 adapters 4 x 20 ml 2 adapters 2x 25 ml Swing bucket rotor type A 8 17 Adapter conical to support 15 ml Falcons for rotor A 8 17 set of 8 Rubber pad for 15 ml round bottom centrifuge tubes set of 8 Set of fuses for 5702 5702 R 230 V 2 x 2 5 A time lag for 5702 5702 R 120 V 2 x 5A time lag Important note 5702 745 006 5702 736 007 5702 737 003 5702 719 005 5702 735 000 5702 724 009 5702 732 001 5702 723 002 5702 717 002 5702 734 004 5702 718 009 5702 709 000 5702 762 008 5702 710 008 5702 711 004 5702 712 000 5702 713 007 5702 716 006 5702 700 002 5702 702 005 5702 701 009 5425 351 003 5425 353 006 Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such action Falcon Registered trademark of Becton Dickinson Cidex Registered trademark of Johnson amp Johnson Duran Registered trademark of Schott Ficoll Registered trademark of Pharmacia Biotech AB Microtainer
39. ngle rotor F 45 18 17 Cryo Aluminum angle 45 18 places max 17 mm for mounting of cryotubes max 17 mm and centrifuge tubes which can be sealed max 16 5 mm max length in mm 50 Adapter for cryotubes max 13 mm and centrifuge tubes which can be sealed max 12 2 mm max length in mm 50 Swing bucket rotors and accessories Swing bucket rotor 4 x 85 ml type A 4 38 aluminum incl 4 round buckets Swing bucket rotor 4 x 85 ml type A 4 38 aluminum excluding round buckets Round bucket 85 ml for A 4 38 set of 4 Round bucket 85 ml for A 4 38 set of 2 Caps aerosol tight for round bucket 85 ml set of 2 5702 000 019 5703 000 012 5704 000 016 5702 704 008 5702 705 004 5702 707 007 5702 706 000 5702 708 003 5702 746 002 5425 715 005 5425 717 008 5425 716 001 5702 747 009 5702 752 002 5702 720 003 5702 740 004 5702 722 006 5702 761 001 5702 721 000 7 Ordering information Adapter for standard tubes and blood sampling systems for round bucket 85 ml 2 adapters 4x 1 5 2 0 ml micro test tubes 2 adapters 5x 1 1 1 4 ml adapter bore x max vessel length in mm 8 5 x 100 2 adapters 5x 2 7ml 12 5 x 100 2 adapters 4x 2 6 7ml 13 5 x 100 4 adapters 4x 4 10 ml 16 x 100 9 adapters 4x 9 15 ml 17 5 x 100 2 adapters 1 x 15 ml Falcon 17 2 x 121 2 adapters 2 x 15 ml Falcon 17 2 x 121 2 adapters 1 x 25 ml 25 x 100 2 adapters 1 x 50 ml Falco
40. ntended use The Centrifuge 5702 5702 R 5702 RH is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes This device may only be operated by trained specialist staff They must have carefully read the operating manual and be familiar with the function of the device 1 2 Delivery package 1 Centrifuge 5702 non refrigerated or 1 Centrifuge 5702 R refrigerated with condensation water tray or 1 Centrifuge 5702 RH refrigerated and heatable each without rotor 1 power cable 1 operating manual 1 rotor key 1 set of fuse 1 3 Installing the device A The device is heavy Lifting and carrying the device can lead to back injuries The device may only be transported in its original packaging gt The device must be transported by least two people gt Use a transport aid e g dolly to transport the device longer distances For 5702 R RH only In order to prevent damage to the compressor following improper transport the device may not be switched on until four hours after setting up For 5702 only Please remove the transport safety device and keep it for possible use if the device is subsequently moved again Place the centrifuge on a solid flat non resonant lab bench For the Centrifuge 5702 R RH the condensation water tray should be inserted from the side see cover flap The surrounding area must be well ventilated and prot
41. ntrifuged When being moved from the cool room to a normal lab environment the centrifuge must either warm up for half an hour in the cool room first or it must warm up for at least 3 hours in the lab before being connected to the supply system in order to prevent damage by condensation The centrifuge must not be moved or knocked while in operation Improperly installed or serviced centrifuges must not be operated Repairs may only be carried out by Service personnel authorized by Eppendorf Use only original Eppendorf spare parts and rotors When handling toxic radioactive liquids or pathogenic microorganisms of risk group see World Health Organization Laboratory Biosafety Manual comply with the relevant national regulations Bioseals are a part of biological safety systems which are not able to guarantee the safety of people and the environment on their own when handling pathogenic microorganisms When working with pathogenic organisms of a higher risk group more than one aerosol tight bioseal must be provided for If the named liquids are spilled in the rotor or rotor chamber the centrifuge must be thoroughly and professionally cleaned Before using any cleaning or decontamination method other than that set out in section 4 Maintenance and cleaning please consult Eppendorf to ensure the intended method will not damage the device Rotors must always be properly secured The centrifuge may only be operated with the rotor firmly tight
42. priate UL Directory Look for the UL Listing Mark on the product Wiiliam R Carney Director North American Certification Programs ut uc Any information amd involving UL Mark services are provided on behalf of UL LLC UL or amy authorized licenses of UL For questions pleas wwa ul Com contacts documentation contact local UL Customer Service Representative at Page 1 of 1 Evaluate your operating manual www eppendorf com manualfeedback eppendorf Your local distributor www eppendorf com contact Eppendorf AG 22331 Hamburg Germany eppendorf eppendorf com www eppendorf com Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG Hamburg Germany 5702 901 016 11 052013
43. r on message appearing briefly in the display signals the reactivation of the faster acceleration and braking ramps 3 13 At set rpm For centrifugation time can be counted either immediately from the start or not until attainment of the preset speed Pressing the START STOP button for longer than 2 seconds with the centrifuge lid open switches the at set rom mode symbolized by the pictogram To exit the at set rom mode again and begin counting down the centrifugation time immediately after starting the centrifuge press the START STOP button again for longer than 2 seconds with the centrifuge lid open until the pictogram ZF is displayed 3 14 Activating and deactivating the signal tone Press and hold down the OPEN button for longer than 2 seconds with the centrifuge lid open to toggle between b on signal tone on and b OF signal tone off Press the OPEN button for less than 2 seconds with the centrifuge lid open to display the current status With the Centrifuge 5702 R 5702 RH the selected signal tone is symbolized by the pictogram d The current status is shown when the OPEN button is pressed for less than 2 seconds while the centrifuge lid is open Centrifuge 5702 3 15 Parameter lock To secure the centrifuge parameters against unintentional adjustment the Centrifuge 5702 and the Centrifuge 5702 R 5702 RH allows you to lock in the parameters you need Press and hold down OPEN and SHORT button simultaneously
44. rature in the rotor During the FAST TEMP run FA appears in the display as well as the present temperature and the fixed optimal rotational speed The run ends automatically or by pressing the STOP button A signal tone sounds periodically Stand by refrige With closed centrifuge lid the rotor chamber is refrigerated before and after a run to ration the selected nominal temperature if this is less than the ambient temperature Since there is no rotation of the rotor the temperature adaptation takes place slowly If the centrifuge was in standby refrigeration over a longer time or at low temperatures start a brief FAST TEMP run before inserting the samples in order to prevent the samples from freezing If the centrifuge is not used for more than 8 hours or the lid is not opened the refrigeration switches off to protect the device Remark In cases of higher ambient temperature temporary air noises may occur until the desired temperature is attained These are a sign of high refrigeration capacity Block the ventilation slots under no circumstances For ambient temperatures below 18 C a warming up time of 1 hour is required before operating 3 Operation 3 19 Heating and refrigeration 5702 RH In the case of the Centrifuge 5702 RH we are dealing with a benchtop centrifuge with a regulated heating and cooling system The Centrifuge 5702 RH can be refrigerated or heated precisely within a temperature range from 9 C to 42
45. rder to avoid breakage of the tubes when using centrifuge tubes of glass polystyrene or similar materials please observe the limiting data with reference to endurance specified by the manufacturer Only load the rotor symmetrically so that the rotor and the pivots are evenly loaded during centrifugation The maximum load adapter tubes and contents of the rotor A 8 17 is 8 x 38 g The maximum permissible tube length is 120 mm 3 Operation 3 6 Operation of round bucket with aerosol tight caps The inner part of the aerosol tight cap is also equipped with a silicone sealing ring Never remove this sealing ring from the cap Whenever using the cap make sure that the sealing ring is undamaged and sits uniformly in the groove Silicone sealing ring The round bucket and the aerosol tight cap are autoclavable 121 C 20 minutes The aerosol tight cap including the silicone sealing ring is subject to natural wear and must be replaced when visual inspection reveals wear When handling the aerosol tight cap remember that polycarbonate has limited chemical resistance to phenol 3 7 Centrifugation with timer setting Switch on the centrifuge with the mains power switch if necessary and then with the switch The nominal values of the last run are displayed Load the rotor symmetrically and close down the centrifuge lid time lt Alters the running time speed Alters the speed in increments of
46. rective europ enne 2002 96 CE relative aux d chets d quipements lectriques et lectroniques DEEE En vertu de cette directive tout appareil livr apres le 13 08 2005 dans le secteur Business to Business dont ce produit fait partie ne pourra plus tre mis au rebut avec les ordures m nag res ou par les services municipaux Pour faciliter leur identification ces appareils seront pourvus du symbole suivant Etant donn que les r glementations relatives l limination des d chets au sein de l UE peuvent varier d un pays l autre nous vous invitons contacter vos fournisseurs au besoin ES Mode d emploi Mettre la centrifugeuse sous tension a l aide du commutateur principal et actionner la touche Standby Les valeurs de consigne de la derni re centrifugation sont affich es Pour le montage du rotor respecter le triangle d indication fix des deux c t s des crous du rotor fig triangle Ils indiquent la direction de la rainure sur la face inf rieure du rotor et sont n cessaires un bon positionnement du rotor Pour ins rer le rotor tourner le tenon d entra nement de l arbre du moteur dans le sens de la longueur Placer le rotor sur l arbre du moteur de mani re ce que les triangles d indication indiquent la m me direction que le tenon d entra nement de l arbre du moteur Serrer fond les crous du rotor l aide de la cl fournie du rotor en tournant dans le sens des aiguilles d une montre Si le serrage
47. rifugeuse et les accessoires Les rotors qui pr sentent des traces de corrosion videntes ou des endommagements m caniques ne peuvent pas tre utilises Contr ler les accessoires chaque mois Eviter tout affaiblissement de la protection du rotor par des substances chimiques agressives telles que des alcalis forts ou faibles des acides forts des solutions contenant du mercure du cuivre ou d autres ions de m taux lourds des hydrocarbures chlor s des solutions salines concentr s ou du ph nol En cas de contamination par des substances agressives nettoyez le rotor imm diatement avec un d tergent doux La densit de la mati re centrifuger ne peut pas d passer 1 2 g ml la vitesse de rotation maximale Dans le cas d une dur e de fonctionnement plus longue les tubes a essai vont se r chauffer Verrouiller le couvercle des tubes avant de proc der la centrifugation Les couvercles ouverts peuvent s arracher lors de la centrifugation et endommager la centrifugeuse Les valeurs seuils sp cifi es par le fabricant de tubes concernant la capacit de charge admissible des tubes en plastique doivent tre respect es En cas d utilisation de solvants organiques par ex ph nol chloroforme la stabilit et la capacit de charge admissible des tubes en plastique peuvent tre r duites Lors de la fermeture du couvercle de la centrifugeuse ne pas poser les doigts en dessous du couvercle au risque de les coincer Les
48. rotors suivants et les nacelles correspondantes ont une dur e de vie maximale de 7 ans La date de fabrication est grav e sous le format 10 01 ce qui signifie octobre 2001 A 4 38 5702 720 003 A 8 17 5702 700 002 Les couvercles herm tiques transparents ont une dur e de vie maximale de 3 ans La date de fabrication est grav e sous la forme d une horloge I e Les couvercles tanches aux a rosols peuvent perdre de leur solidit sous l effet de solvants organiques par ex ph nol chloroforme Contr ler r guli rement si les couvercles herm tiques ne pr sentent pas de traces de corrosion chimique ou de petites fissures Les couvercles herm tiques pr sentant des fissures ou d autres traces de corrosion doivent tre imm diatement remplac s N Les rotors couvercles ou nacelles qui sont endommag s sous l effet de r actions chimiques ou m caniques ou qui ont d pass leur dur e de vie maximale ne peuvent plus tre utilises Remise du produit un tiers Si vous mettez ce produit disposition d un tiers veillez y joindre le pr sent mode d emploi Elimination du produit Il convient de se conformer aux r glementations l gales applicables pour l limination du produit Informations concernant la mise au rebut des appareils lectriques et lectroniques dans l Union europ enne Au sein de l Union europ enne les appareils lectriques sont r gis par des r glementations nationales bas es sur la di
49. s e Les r parations devront tre confi es un atelier apr s vente d ment accr dit par la soci t Eppendorf Seule l utilisation de pi ces de rechange et des rotors d origine Eppendorf est autoris e En cas de manipulation de liquides toxiques ou radioactifs ou de germes pathog nes de la cat gorie de risques v Organisation Mondiale de la Sant Laboratory Biosafety Manual les dispositions l gales nationales doivent tre respect es En cas de germes pathog nes avec un groupe de risques plus lev s il est n cessaire de pr voir une mesure plus importante qu un scellement bio tanche aux a rosols Si un liquide des types indiqu s est r pandu par m garde dans le rotor ou dans le bol du rotor la centrifugeuse doit faire l objet d un nettoyage soign par un personnel comp tent Les rotors doivent tre toujours fix s en tenant compte des prescriptions La centrifugation ne peut tre effectu e que si le rotor est correctement serr Le rotor doit tre charg de mani re sym trique les tubes diam tralement oppos s doivent tre du m me type et remplis de fa on similaire Mode d emploi succinct Avant d effectuer la centrifugation il convient de v rifier visuellement dans chaque cas un ventuel endommagement des r cipients Les r cipients endommag s ne doivent pas tre centrifug s puisque si ceux ci se cassent il peut y avoir outre la perte de l chantillon d autres dommages sur la cent
50. s are inserted in the bores of the rotor opposite one another in pairs To ensure that the rotor is loaded symmetrically the tubes opposite one another need to contain approximately the same filling quantity Fixed angle rotors are operated without a rotor lid 29 0 0 40 Operation 30 3 Operation 3 5 Swing bucket rotors The swing bucket rotor A 4 38 can be equipped with round or rectangular buckets Round buckets and the associated adapters see ordering information are conceived for the centrifugation of Falcons blood withdrawal systems and other centrifuging tubes with round bottoms Rectangular buckets and associated adapters see ordering information are conceived for the centrifugation of glass centrifugation tubes UN Use only the rotor bucket round or rectangular adapter combinations approved by Eppendorf Check that all buckets are fully attached lubricated lightly and can swing out freely If you use overlength tubes it is essential to carry out a manual swing test with empty tubes When the rectangular buckets are not completely filled insert the adapters so that the bolts of the rotor A 4 38 are symmetrically loaded Avoid unbalanced loading of the inner adapter bores pointing to the rotor nut since this could cause overshooting of the rectangular buckets For mechanical stability all the places must be fitted with identical buckets The buckets are sorted by weight The weight class is embo
51. ser refroidir le moteur uniquement 5702 Er 24 Erreur dans le groupe de Au d but de la centrifugation prendre contact avec le refroidissement SAV Apres de nombreuses centrifugations laisser refroidir la centrifugeuse Si aucune des mesures pr conis es ci dessus ne devait apporter de rem de aux anomalies il convient de faire appel au service apr s vente den Service 46 ce C a ce CE ce ce ce CE CE ge CE ce rec el re CES ce ame CE a ce CE ee CE Le ce ES ES Ce Ce ec ce eS ce a ce ce ec C C CE ng ce ce ce ce ce ce ce ce ce 4 ce ce Ee ce ce Ce Ee et e ee Ce CE ve CE ce CS ce ce ce ce PL sc PA Ce AAA 6 re CE ce at ce re ce rc ce gt g C CE CE ce Ce CE tn ce a CE z ce 4 3 KA EG Konformit tserkl rung eo ce ce ce EE EC Conformity Declaration ec A 6 ce Ce lt ce ce tere Das bezeichnete Produkt entspricht den einsch legenden Anforderungen A ve ce vr p Bor 0 kap EG Richtlinien und Normen heei oe re ruse JR e Se ce Co ce x uktes oder einer nicht bestimmungsgema en Anwendung verliert diese Erkl rung ihre G ltigkeit ri ra c el The product named below fulfi di eee ce the EC directives and standards listed OC MR ce 6 LE ce ce or an unintended use this declaration becomes invalid ce Tr C ce re ce ce ec YA ce ce ce c Ce Tr ce Produkt
52. ssed on the side of the bucket e g 90 the last 2 digits in grams Opposing buckets must be of the same weight class When reordering please quote the weight class required Before inserting the buckets in the grooves make sure the grooves are clean Dirty grooves or pegs will prevent the buckets from swinging out evenly Fig 4 Fig 5 Fig 6 Rotor and buckets symmetrically Rotor and buckets asymmetrically Adapter loaded asymmetrically loaded loaded Not permitted because the Not permitted because the pegs of the bucket are not uniformly loaded and the loading of the adapter bores pointing to the rotor nut could cause overshooting of the rectangular buckets pegs of the buckets are subject to differing loads The specified max weight of 400 g imprinted on the rotor is the gross weight rating of a bucket including adapter tubes and contents The maximum load adapter tubes and contents of the round bucket is 190 g The maximum load adapter tubes and contents of the rectangular bucket is 240 g A maximum of 8 x 15 ml Falcons or 8 x 15 ml tubes of Duran glass can be centrifuged in the rotor A 8 17 Prior to the centrifugation of 15 ml Falcons please insert the provided plastic adapter into the rotor Prior to the centrifugation of tubes of Duran glass please insert the round rubber plate into the rotor Please make certain prior to centrifugation that such a rubber plate is found under every glass tube In o
53. temp Starts a temperature control run in order to reach the preselected temperature as quickly as possible During the temperature control run FA appears in the display as well as the current temperature and the optimum rotational speed Refrigeration runs at less than room temperature are automatically carried out with a rotor specific low rotational speed while heating runs are carried out initially with a maximum and then with a nominal rotational speed For runs with precisely controlled temperatures we recommend starting a short Fast Temp run immediately prior to centrifugation This prevents an overshooting of the temperature in the rotor chamber such as can sometimes occur after longer standby periods Stand by The rotor chamber is heated or cooled to the selected nominal temperature both Heating and before and after a run with the lid closed Because the rotor does not thereby rotate refrigeration the temperature adjustment occurs slowly The current temperature in the rotor chamber is displayed For runs with precise temperature control following longer standby periods we recommend starting a brief Fast Temp run immediately prior to centrifugation This prevents potential overshooting of the temperature in the rotor chamber 3 20 Opening the centrifuge in case of power failure Pull the power plug and wait for the rotor to come to a stop this may take as long as 5 minutes Emergency lid release on underside of device by pull
54. ugh cleaning remove the rubber plates from tube holders and buckets and clean all parts separately Then refit the rotor and bolt it into place with the supplied rotor key Check the rotor tube holders and buckets once a month for mechanical damage All rotors buckets adapters caps and tube holders can be autoclaved 121 C 20 min The aerosol tight cap including silicon sealing ring rubber mats and rectangular bucket adapters are subject to normal wear and must be replaced when a visual inspection reveals wear Do not unbolt the rectangular bucket adapters from each other On the swing bucket rotor make sure in particular that the pegs and grooves of the buckets are free of dirt They should be lightly lubricated with pivot grease provided with each swing bucket rotor so the buckets can swing freely The aerosol tight buckets must not be stored with their caps sealed 4 3 Refrigerated centrifuges Clean condensation water and ice buildup regularly from the rotor chamber by defrosting using a soft absorbent towel Regularly empty and clean the condensation water collector Remove this from the left Please clean the condensation water drain regularly 4 Maintenance and cleaning 4 4 Glass breakage When centrifuging glass tubes please observe that high speeds rcf s increase the risk of glass breakage Please follow the manufacturer s instructions concerning the maximum speed rcf of centrifuge tubes In the event of
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