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Centrifuge 5804 / 5804 R / 5810 / 5810 R 1

1. 31 yuns 10 dW3 p IPON Instrucciones breves 32 Instrucciones breves Las centr fugas 5804 5810 son centr fugas de mesa sin refrigerar y las 5804 R 5810 R son centr fugas de mesa refrigeradas La Centrifuga 5804 5804 R 5810 5810 R est determinada exclusivamente para la utilizaci n en espacios interiores y sirve para la separaci n de soluciones acuosas y suspensiones de diferente densidad en tubos de muestras autorizados Los modelos 5804 5804 R tienen una capacidad volum trica m xima de 400 ml y los modelos 5810 5810 R una capacidad m xima de 1 600 ml Antes de poner el aparato en marcha por primera vez leer el manual de instrucciones Para obtener una visi n general m s completa del manejo es recomendable leer adem s la versi n en ingl s m s detallada p ginas 5 a 25 La gu a de accesorios del anexo forma parte de las presentes instrucciones de servicio Este signo lo encontrara en la centrifuga asi como en varios puntos de estas instrucciones de servicio Los textos marcados de esta manera se refieren a indicaciones relevantes para la seguridad Unicamente debe utilizar la centrifuga si ha leido estas indicaciones de seguridad Indicaciones de seguridad En inter s de su propia seguridad por favor tenga en cuenta las siguientes disposiciones El rotor y su tapa tienen que estar siempre sujetos de acuerdo con las normas Unicamente debe centrifugarse con el rotor firmemente apretado E
2. Centrifuge 5804 5804 R 5810 5810 R Instruction Manual Mode d emploi succinct Instrucciones breves eppendorf Centrifuge 5804 5804 R 5810 5810 R Shortcuts Task Function Lid Press Display Chapter in Centrifuge instruction 5804 R 5810 R manual Parametarset 1 Select 1 Selected Ais E S at parameter y flashes E 2 Select 2 New nominal a oF v value appears 1 Press repeatedly Acceleration 3 11 SOT Stop CI ramp 9 fast L 0 slow E 2 Select ramp a Deceleration ramp 9 0 OCHO Alarm ON OFF Dj O alarmon on Y alarm off m Programming 1 Set parameter 1 Parameter shown 3 14 only during standstill w 2 Press 2 x 2 P symbolizes L first free program no 3 Storing 3 ok gt 2 sec x 3 12 At set rom 4 f on E y open gt 4 sec K off Centrifuge 5804 5804 R 5810 5810 R Figure 1 2 rpm 0 rcf QO rad prog rpm rcf Y accel brake min sec Fig 1 Display field and control panel of the 5804 5810 non refrigerated rpm ae rcf GQ rad C prog rpm rcf ee JS accel MX brake min sec Fig 2 Display field and control panel of the 5804 R 5810 R refrigerated a Q DA Contents Centrifuge 5804 5804 R 5810 5810 R Centrifuge 5804 5804 R 5810 5810 R Operating Instructions 22 424588 a a aa A WR ards d
3. Mode d emploi succint 26 Mode d emploi succint Les centrifugeuses 5804 5810 sont des mod les de paillasse non r frig r s et les centrifugeuses 5804 R 5810 R sont des mod les de paillasse r frig r s La Centrifugeuse 5804 5804 R 5810 5810 R doit exclusivement tre utilis e a l int rieur Elle sert s parer les solutions et les suspensions aqueuses de diff rentes densit s dans des tubes autoris s Les mod les 5804 5804 R ont une capacit de charge maximale de 400 ml les mod les 5810 5810 R de 1600 ml Avant de proc der a la premi re mise en service des centrifugeuses 5804 5810 et 5804 R 5810 R il convient de lire le mode d emploi Pour un aper u complet des instructions d emploi nous vous recommendons de lire en outre la version en anglais pages 5 25 plus compl tes A Vous trouverez ce symbole sur votre centrifugeuse ainsi qu diff rents endroits de la notice d utilisation Celui ci indique la pr sence de consignes de s curit importantes N utiliser la centrifugeuse qu apres avoir lu ces consignes des curit Instructions de s curit Pour la propre s curit des utilisateurs il convient de respecter strictement les instructions ci apr s Les rotors et les couvercles de rotor doivent toujours tre fix s conform ment aux instructions Ne jamais centrifuger sans avoir v rifi le serrage de la fixation du rotor Le rotor doit uniquement tre garni de mani re sym triq
4. Veuillez contr ler r guli rement si de tels couvercles ne pr sentent pas de corrosion chimique ou de petites fissures Les couvercles pr sentant des fissures ou des d colorations laiteuses doivent tre imm diatement remplac s Les rotors couvercles ou nacelles qui sont endommag s sous l effet de r actions chimiques ou m caniques ou qui ont d pass leur dur e de vie maximale ne peuvent plus tre utilis s PC Polycarbonate PP Polypropyl ne Installation AN Ne concerne que le mod le 5804 R 5810 R attendre au moins 4 heures avant la premi re mise sous tension de la centrifugeuse pour viter d endommager le groupe compresseur et qui serait la cons quence d un transport non conforme La tension et la fr quence du r seau doivent correspondre aux donn es fournies sur la plaque du fabricant de l appareil Placer la centrifugeuse sur une surface horizontale stable et exempte de r sonance en cas de forte vibration du support le dispositif de s curit anti balourd peut tre rendu inactif S assurer d une bonne ventilation de l environnement de la centrifugeuse et viter toute incidence solaire directe Respecter un espace libre d au moins 15 cm sur les c t s de la centrifugeuse et de 10 cm l arri re Selon les recommandations de la norme EN 61010 2 020 il convient de marquer et de respecter un espace libre de s curit de 30 cm tout autour de la centrifugeuse Cet espace devra
5. Activa la preselecci n de la velocidad En la pantalla parpadea SPEED velocidad Activa cambio a visualizaci n FOR o a la correcci n del radio Q Modifica los valores En el campo de indicaci n aparece el nuevo valor nominal Activa la preselecci n del tiempo 1 99 min o funcionamiento continuo Pantalla 00 Activa la programaci n de los niveles de aceleraci n y frenado Y YX Activa la preselecci n de la temperatura de centrifugaci n y la refrigeraci n en standby Rango de temperaturas de 9 C a 40 C Inicia la centrifugaci n BE parpadea y muestra la rotaci n del rotor Inicia la identificaci n manual del rotor Detiene la centrifugaci n Interrumpe el programa en curso escape Borra el programa seleccionado Inicia una regulaci n r pida de la temperatura a la velocidad espec fica del rotor Finaliza al llegar a la temperatura nominal gt Se al ac stica intermitente Activa el programa memorizado Inicia la nueva programaci n P1 P9 PA PZ Inicia la marcha breve Mantener pulsada la tecla Short durante el tiempo que debe durar la marcha En la pantalla aparece el letrero SH At set rpm produce una cuenta atr s del tiempo de centrifugado al alcanzarse el n mero de revoluciones preseleccionado at set rpm Si se pulsa la tecla START 7 STOP durante m s de 4 s con la tapadera de la centr fuga abierta se produce una conmutaci n a la funci n at set rpm Con la tapa cerra
6. Eppendorf s r l Tel 390 2 55 404 1 Fax 390 2 58 013 438 E Mail eppendorf eppendort it Internet www eppendort it JAPAN Eppendorf Co Ltd Tel 81 3 5825 2363 Fax 81 3 5825 2365 E Mail info eppendorf jp Internet www eppendorf jp NORDIC Eppendorf Nordic Aps Tel 45 70 22 29 70 Fax 45 45 76 73 70 E Mail nordic eppendorf dk Internet www eppendorf dk SOUTH 8 SOUTHEAST ASIA Eppendorf Asia Pacific Sdn Bhd Tel 60 3 8023 2769 Fax 60 3 8023 3720 E Mail eppendorf eppendorf com my Internet www eppendorf com my SPAIN Eppendorf Ib rica S L U Tel 34 91 651 76 94 Fax 34 91 651 81 44 E Mail iberica eppendorf es Internet www eppendorf es SWITZERLAND Vaudaux Eppendorf AG Tel 41 61 482 1414 Fax 41 61 482 1419 E Mail vaudaux vaudaux ch Internet www eppendorf ch UNITED KINGDOM Eppendorf UK Limited Tel 44 1223 200 440 Fax 44 1223 200 441 E Mail sales eppendorf co uk Internet www eppendorf co uk USA Eppendorf North America Inc Tel 1 516 334 7500 Fax 1 516 334 7506 E Mail info eppendorf com Internet www eppendorfna com OTHER COUNTRIES Internet www eppendorf com worldwide eppendorf In touch with life Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 538 01 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc One Cantiague Road PO Box 1019 Westbury N
7. Y 11590 0207 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe International Tel 49 1803 666 789 E mail support eppendorf com North America Tel 1 800 645 3050 ext 2258 E mail support naGeppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support asiapacificGeppendort com B 5820 900 105 08 0209 Printed in Germany Printed on chlorine free bleached paper eppendorf is a registered trademark
8. actives ne doivent pas tre centrifug es La densit de 1 2 g ml ne peut pas tre d pass e lors de la centrifugation a r gime maximal En cas de dur e plus longue de fonctionnement dans les centrifugeuses non r frig r es 5804 5810 les r cipients d chantillonnage se r chauffent Les donn es limites sp cifi es par le fabricant des r cipients propos de la charge admissible doivent tre respect es En cas d utilisation de solvants organiques par ex chloroforme la solidit des r cipients en plastique peut tre diminu e En cas de passage du chambre r frig r e a un laboratoire normal soit la centrifugeuse doit tre pr chauff e 1 2 heure dans la chambre r frig r e soit elle doit se r chauffer au moins pendant 3 heures dans le laboratoire et ne doit pas tre raccord e au secteur avant d viter des dommages en raison du condensat Les rotors sont des composants de qualit sup rieure devant r sister a des charges extr mes Les rotors en aluminium sont prot g es tr s largement contre la corrosion de produits chimiques courants des laboratoires grace une couche anodis e cette protection n est cependant pas illimit e Veuillez prot ger les rotors des d t riorations m caniques M me de l g res fissures ou rayures peuvent entrainer des d t riorations internes graves du mat riel qui sont visuellement difficilement reconnaissables voire m me compl tement non reconnaissables Veuillez viter
9. de r duire leur protection par des produits chimiques agressifs par exemple des alcalis forts et faibles des acides forts des solutions contenant des ions de mercure de cuivre et d autres m taux lourds de Phydrocarbure chlor e des solutions sal es concentr es Mode d emploi succint Lors de manipulation de liquides toxiques ou marqu s sur un plan radioactif ou de germes pathog nes du groupe de risques II voir World Health Organization Laboratory Biosafety Manual les r glementations nationales correspondantes doivent tre respect es En cas d impuret s dues a des solutions agressives veuillez nettoyer imm diatement le rotor imm diatement l aide d un produit de rin age neutre Ceci est en particulier valable pour les al sages des rotors a angle fixe et les gobelets Les rotors et les godets nacelles correspondants ont une dur e de vie maximale de 7 ans la date de fabrication est grav e sous le format 10 98 qui signifie octobre 1998 A 4 44 5804 730 003 A 4 62 MTP 5810 711 002 A 2 DWP 5804 740 009 T 60 11 5804 730 003 F 34 6 38 5804 727 002 A 4 81 5810 718 007 A 4 62 5810 709 008 A 4 81 MTP 5810 725 003 Les couvercles des rotors et les capuchons des gobelets rectangulaires en PC ou en PP ont une dur e de vie de 3 ans La date de fabrication est grav e sous la forme d une horloge CS Les couvercles transparents peuvent perdre de leur solidit sous l effet de solvants organiques ph nol chloroforme
10. in blue in an alternating sequence The activated at set rom function is symbolized by the continuously illuminated upper blue triangle inthe pictogram 2 In order to leave th at set rpm function again and thus to activate the countdown of the centrifugation time directly after switching on the centrifuge press the START 7 STOP key while the centrifuge lid is open The standard setting is selected again after 4 seconds symbolized by the illumination of the lower triangle in the pictogram Pa T 15 bunje19d0 Operating 90 6 16 3 Operating 3 13 Preset program Programs can only be preselected when the device is at a standstill Press this key once gt the program no that has been set flashes 0 Data from last run 1 9 A Z Fixed programs select further program numbers Returns to program 0 or leaves the programming mode when the lid is open In order to start the selected program immediately the centrifuge lid must be closed prior to pressing the START STOP key 3 14 Programming Storing a fixed program only possible when the device is at a standstill It is possible to save a maximum of 35 fixed programs 1 9 A Z Enter the program data to be used first by pressing the parameter keys and the arrow keys or use the data from the last run The at set rpm functions and the set deceleration ramp can also be saved in a program if necessary Press this key twice gt the first free program no
11. indicated by P appears in the display and flashes The desired free program number 1 9 A Z can be selected Hold down this key for two seconds until ok appears in the display The previously set parameters of Temp Speed Time etc are now saved as a data set 8000 If parameters are modified during the run with a fixed program 0 appears in the PROG field and the user exits the program without it being modified To exit the fixed program call up program 0 or modify the parameters 3 15 Write protection In order to avoid accidental deletion of an existing fixed program the old data set must be deleted while the lid is open prior to renewed assignment of a program number Press this key once gt the program no display flashes select the program no which is to be deleted Press this key within ten seconds until cleared appears 800 O 3 Operating 3 16 Nominal value display All nominal values are displayed when the centrifuge is at a standstill During a run all nominal values can be displayed for 2 5 seconds by pressing one of the parameter keys Temp Speed Time 3 17 Automatic rotor recognition Automatic rotor recognition occurs at the beginning of each run When a nevv rotor is recognized the maximum rotational speed appears in the display for two seconds If the nominal speed set is greater than the maximum speed for the rotor used it is aligned to the maximum speed and the run is interrupt
12. is automatically switched off after 8 hours in order to protect the device The switching of standby cooling to permanent operation is carried out at own risk and can lead to overheating of the compressor Please empty and clean the tray for condensation water on the right at the bottom of the device on a regular basis AN Remove any condensation vvater and ice regularly from the rotor chamber using a soft moist cloth Defrosting is recommended for ice removal 3 Operating 3 10 Preset radius The internal conversion of rotational speed to RCF occurs as a standard with the largest radius A smaller radius can be entered for another adapter Press this key repeatedly until the radius symbol G appears to the left of the SPEED display speed The radius value flashes change the values entered The new rcf value appears three seconds or 10 s during the stillstand after the rotor radius has been entered 00 O 3 11 Centrifugation with Soft start stop If the maximum acceleration deceleration speed level 9 is not desired it is possible to set the speeds in nine different levels Press this key repeatedly until 4 the symbol for the acceleration levels appears next to the TIME display Presetting the acceleration level 9 0 Deceleration is set in the same way XY Braking level O corresponds to free deceleration For levels 0 8 the symbols appear in the display 00 Q Approximate braking times
13. symmetrically Fig 6 Not allowed because the pegs are not evenly loaded loades rotor is asymmetrically loades The rectangular buckets are sorted according to weight The weight category is printed on the side in the groove e g 86 the last two places in grammes Opposing buckets should be of the same weight category When placing follow up orders please state the weight category This also applies to titer plate buckets Ensure that the grooves are clean before inserting the buckets Unclean grooves and pivots prevent the buckets from swinging freely When working with a rotor which is not completely loaded position the tubes within the buckets in such a way that the rotor pivots are stressed evenly Fig 7 Rotor is symmetrically loaded Fig 8 Not permitted pivots are not stressed evenly rotor is not symmetrically loaded A manual swing test with empty tubes must be carried out if oversized tubes i e longer than 100 mm are used 11 bunje19d0 Operating 12 3 Operating Permitted loads for the svving bucket rotor 100 mi rectangular bucket adapter tubes adapter tubes Rotor A 4 44 A 2 DWP A 4 62 A 4 81 4 x 100 ml Deepwell 4 x 250 ml 4 x 400 ml plate rotor A 4 62 MTP A 4 81 MTP Maximum load per 310g 6
14. the lid onto the rotor The rotor lid need not be screwed tight 3 4 Loading the rotors The rotors and buckets must always be loaded symmetrically The adapters may only be loaded with the test tubes recommended Differences in the weight of the filled sample tubes should be kept as low as possible in order to prolong the life time of the drive and to minimize running noises caused by imbalance On each rotor you will find information concerning the maximum weight of a completely loaded bucket bucket including adapter tubes and liquid or titer plate buckets including titer plate and liquid see also applicational limitations Performance data of the rotors Type max capacity max rotational max 5804 5810 Aerosol tight Designation mi speed rpm Radius 5804 R 5810 R centrifugation max rcf x g cm possible Swing bucket rotors A 4 81 1 4 x 400 ml 4 000 3 250 18 0 e e A 4 81 19 96 x 2 ml 4 000 2 600 14 6 e A 4 81 MTP 7 16 x MTP Platte 4 000 2 900 15 3 e A 4 62 2 4 x 250 ml 4 000 3 200 18 0 o e A 4 62 MTP 2 16 x MTP Platte 4 000 2 750 15 4 e A 4 44 4 x 100 ml 5 000 4 500 16 1 e e e A 2 DWP 4 x Deepwell Platte 3 700 2 250 14 7 e e Fixed angle rotors F 34 6 38 6 x 85 ml 11 000 15 550 11 5 e e F 34 6 38 6x85 ml 12 000 18 500 11 5 only 5810 R F 45 30 11 30 x 2 ml 14 000 20 800 9 5 e e FA 45 30 11 30 x 2 ml 14 000 20 800 9 5 e e
15. up Observe the limiting data specified by the tube manufacturer The use of organic solvents e g chloroform may have an adverse effect on the stability of plastic test tubes Following operation in a cooling room run the centrifuge for 30 minutes in the cooling room until it is warm Alternatively allow it to warm up in a lab for at least three hours but do not plug in the centrifuge in order to prevent damage caused by condensation Rotors are high grade components which are subject to extreme mechanical strain Aluminium rotors are protected against corrosion caused by commonly used laboratory chemicals by means of an electrolytic coating although this protection cannot be fully guaranteed Please ensure that the rotor is protected from mechanical damage Even slight scratches and cracks can cause severe inner damage to the rotor materials which are difficult or impossible to detect with the eye Please avoid using aggressive chemicals with the rotors Such chemicals include concentrated and mild alkalis concentrated acids solutions containing mercury ions copper ions and other heavy metal ions chlorinated hydrocarbons and concentrated saline solutions When handling toxic or radioactive liquids or pathogenic bacteria out of Risk Group II see World Health Organization Laboratory Biosafety Manual observe national regulations In the event of contamination caused by impurities or aggressive agents the rotor must be cleaned im
16. v r ii a ba S 22 SjUJJUOJ Introduction 1 Introduction The Centrifuges 5804 5810 are non refrigerated bench top centrifuges and the Centrifuges 5804 R 5810 R are refrigerated bench top centrifuges The Centrifuges 5804 5804 R 5810 5810 R are intended exclusively for use indoors and are for separating aqueous solutions and suspensions of differing density in approved test tubes The 5804 5804 R models have a maximum capacity of 400 ml and the 5810 5810 R models have a maximum capacity of 1600 ml A This sign is found on your centrifuge and on several pages in the operating manual Texts labeled with this sign contain safety notes Read these safety precautions before using the centrifuge for the first time Before starting up the Centrifuges 5804 5810 and 5804 R 5810 R for the first time please read the instruction manual 1 1 Delivery package 1 Centrifuge 5804 5810 with fan cooling or 1 refrigerated Centrifuge 5804 R 5810 R rotor not included with centrifuges 1 Main power cable European standard plug 1 Instruction manual 1 Rotor key 1 2 Unpack When the machines are removed from the packaging they must be supported by two persons near the rubber feet at the bottom on the left and right hand side and then carried the short distance to the lab bench Please observe the weight bearing capacity of the bench If the machines are to be transported over long distances in the lab a trolley must be use
17. 30 g 780 g adapter tubes Maximum load per plate carrier 380 g vvith full plates 380 g vvith full plates 380 g vvith full plates Maximum load per 50 ml Falcon bucket 144 g for two conical tubes incl liquid and form insert Specifications on the rotor 4x0 6kg 2 x 1 010 kg 4x1 1kg 4 x 1 4 kg weight for loaded buckets A 4 81 rectangular buckets 4x 1 22 kg A 4 81 MTP The weight specifications printed on the rotor e g 4 x 0 6 kg for the 4 x 100 ml swing bucket rotor A 4 44 correspond to the overall weight of the rectangular bucket inclusive of adapter test tubes and the contents of each individual position In general commercially available deepwell plates with a 96 well plate format may only be used in the buckets of the A 4 62 MTP on condition that the maximum load does not exceed 380 g and the maximum height of the plate does not exceed 53 mm This restriction also applies to stacked titer plates or culture plates Commercially available deepwell plates or filter plates filter plate stacks in the 96 well plate format may be inserted in the buckets of the A 2 DWP only if their load does not exceed 380 g and their height does not exceed 89 mm These restrictions also apply to stacked titer plates or culture plates Commercially available deepwell plates or filter plates filter plate stacks may be inserted in the buckets of the A 4 81 MTP only if their lo
18. G field The run is then ended automatically at the arrived at nominal temperature or by pressing the Stop key A signal can be heard at regular intervals The centrifuge does not terminate the run until the rotor carrier or rectangular bucket have reached the pre selected temperature The target temperature is initially measured on the chamber and then shown in the display However the temperature run is automatically terminated when the rotor carrier or rectangular bucket have reached the target temperature When the lid is closed the rotor chamber is refrigerated to the preselected nominal temperature before or after a run as long as this value is below ambient temperature The rotor does not turn during this procedure and the temperature changes more slowly If the centrifuge is not used for longer than 8 hours or if the lid is not opened for this time the refrigeration function switches off for safety reasons Standby off appears in the display together with the nominal temperature in the rotor chamber The desired temperature can be reached rapidly via the Fast Temp function The standby cooling of the centrifuge can also be switched on permanently To do this the Temp and Prog keys must be pressed simultaneously Standby 8h appears in the display Following this the Fast temp must be pressed immediately in order to switch the standby cooling over into permanent operation Standby endless appears in the display AN The standby cooling
19. Ge Nd V fh E s 15 3 13 Pr se programs Le cues A a e Roane edd a amp SR E a RS SA d 15 3 14 Programming ir a e 16 3 15 Write protections asias aid e A Ad Pa a n ee 16 3 16 Nominalivalue display 2 42 date ad Goa d a whe BEG nj e be ea 16 317 Automatic rotor recognition s 224888 de d Wea dd do vd dr i sada te 16 3 18 Display ot elapsed spin times da dia det a A antennes de 17 3 19 Switching on off the warning signal 17 3 20 Exiting the Service program dia ate shen sd dm dt A Sey ene ld we aan 17 3 21 Control via serial interface optional 0 0 ketene 17 3 22 Opening the centrifuge in the case of a power failure 17 923 Overload cutolutswitchi FUSES dua ii ne eos ee Meee eee ee he ee we 17 4 Maintenance and cleaning lt gt 46282828 ponte die eagle ere ne one E eee eee a ene ated ene 18 4 1 DEVICE iii a ME it A A Ne DRM NE ee 18 4 2 MBSSHOLORSE sa sot iii a a as dao N ce 18 4 3 The acrosol tight rotor ii ii A o dh e 18 4 4 Rotor SteriliZation iii ii dood a A aia P 18 4 5 Glass breakages i ris cence io A A AA AO A A IA DE RR 19 4 6 Refrigerated ContrifUJES 2 nse van ir a A A A A A A e 19 4 7 Return ot devices se disi im e a h ido ee 19 5 Troubleshooting cc dd snn ue s Kri q s di d dd dae Sede d ale eee hence 20 6 Technical data scan rar a a h are due 21 7 Ordering information d k sd s 082
20. a y el adaptador para evitar da os posteriores De lo contrario los fragmentos finos de vidrio ara an la superficie del rotor y los contenedores con lo que disminuye su resistencia a las sustancias qu micas En la c mara de centrifugaci n y debido a los remolinos de aire se forma una abrasi n met lica negra muy fina que adem s de da os en la c mara de centrifugaci n el rotor los contenedores y el adaptador origina tambi n impurezas en las muestras Por favor revise regularmente los orificios del rotor en cuanto a residuos y da os Devoluci n de aparatos Por favor para devolver las centr fugas preste atenci n a que los aparatos est n completamente descontaminados con el fin de que no representen ning n riesgo para la salud de nuestro personal de servicio En www eppendorf com hallar m s informaci n y un impreso de confirmaci n de descontaminaci n Para un m todo de descontaminaci n adecuado consulte tambi n con los responsables de seguridad de su laboratorio Por favor rellene la confirmaci n de descontaminaci n y acomp ela con el aparato en caso de que ste deba ser devuelto a Eppendorf Esquema para la localizaci n de fallos Centrifugeuses 5804 5804 R y 5810 5810 R Error Pantalla Causa Eliminaci n En la pantalla no Nada No se ha enchufado a la red Controlar el cable de red aparece nada Fallo en el suministro el ctrico Comprobar el fusible del aparato y del labora
21. ad does not exceed 380 g and their height does not exceed 60 mm These restrictions also apply to stacked titer plates or culture plates If deepwell plates used with dimensions which do not correspond to the values stated above e g prototypes combination plates with a heavy lid or specially designed plates the user must consult Eppendorf to establish the suitability of using these plates in a titer plate or Deepwell plate rotor Under no circumstances use MTP MTP Flex and DWP buckets with deformed side plates 3 5 Routine centrifugation with preset time rcf display Turn on the main power switch The nominal values of the last run are displayed Load the rotor symmetrically and close the centrifuge lid The Open key lights blue speed activates the preset speed The SPEED display flashes modify the values The new nominal value appears in the display If the preset speed is in rcf please check the radius that has been entered see Section 3 10 Oo Q 3 Operating to select rcf press this key repeatedly until the rcf symbol appears to the left of the SPEED display speed The rcf value flashes and can be modified using the arrow keys activates the preset time The TIME display flashes The time can be modified using the arrow keys activates the preset temperature The nominal temperature in C can be modified using the arrow keys starts the run NE flashes and displays the rotation of the rotor 6000 Rot
22. amber should only be cleaned with a moist cloth After cleaning with detergent the rubber seals in the rotor chamber should be rinsed well with water and lubricated with glycerine in order to prevent the seals from becoming brittle If condensation water forms in the rotor chamber after freezing occurs dry with a soft absorbent cloth The user must consult the manufacturer before cleaning or decontaminating the centrifuge using methods not recommended by the manufacturer in order to ensure that the centrifuge and accessories are not damaged To ensure that the centrifuge functions correctly and safely in the long term please note that aggressive chemicals can damage the rotor buckets and boiler Please check the centrifuge regularly for damage caused by corrosion 4 2 The rotors The rotor and buckets must be cleaned regularly to prevent contamination caused by residue Check at least the rotor and housing monthly for residue and corrosion This applies in particular to the rotor bores Please clean your rotor using a neutral cleaning liquid This will protect the rotor and prolong its service life As a reminder the message clean rotor appears in the display of the centrifuge three times after every 200 runs When using the swing bucket rotor ensure that the grooves in which the buckets are fitted are free of contamination The buckets can be lubricated with the lubricant grease for pivots supplied although care must be taken to ensure th
23. and in the laboratory clean rotor 200 runs Clean rotor and drum Standby off Centrifuge not used for 8 hours Open lid and then close it again Centrifuge does not no rotor No rotor Mount rotor start up Error in the drive or rotor Switch off the device and switch it on recognition again Lid cannot be None Power failure Bring rotor to a standstill activate opened emergency lid release Lid not closed Press Open Lid latch not engaged Press Open and close lid again completely Close Lid Lid not closed correctly Press down lid Lid does not open Lift Lid Lid does not open automatically Open lid manually Centrifuge shakes IMBAL Rotor not loaded symmetrically Check rotor equipment and rotor load during acceleration ROTOR Rotor not fastened Fasten rotor correctly and switches off Device has been moved or work Place device on stable work surface surface unstable Centrifuge switches SPEED Nominal speed for rotor too high Enter new nominal speed off Error 1 Rotor is not recognized Repeat run If error recurs test with Problem with the rotational speed anotherrotor measuring system Error 2 Imbalance sensor damaged Repeat run Error 3 Problem with the rotational speed Leave device switched on for 8 min press measuring system Open then open the device Repeat run Error 4 Lid latch sensor damaged Switch device off and then switch it on again Repeat run Error 5 Unauthorized opening oflidorlid Repeat run switch damaged over
24. at the buckets can still swing freely 4 3 The aerosol tight rotor The sealing rings of the aerosol tight rotor FA 45 30 11 are subject to natural wear and tear and should be replaced regularly if damaged To protect the rotor please ensure that the sealing rings are maintained regularly When handling the rotor lid please observe the specifications regarding the chemical resistance of the materials of construction The cover of the aerosol tight rotor must not be fastened tightly during storage 4 4 Rotor sterilization All rotors are autoclavable 121 C 20 min After the rotor has been autoclaved ten times the lids of the aerosol tight buckets or rotors must be exchanged The aerosol tight Rotor FA 45 30 11 and the lid can be autoclaved at 142 C for 2 hours to destroy prions but the aerosol tight lid must be exchanged after each autoclaving 4 Maintenance and cleaning 4 5 Glass breakage When centrifuging glass tubes please observe that high speeds rcf s increase the risk of glass breakage Please follow the manufacturer s instructions concerning the maximum speed rcf of centrifuge tubes In the event of glass breakage carefully remove all splinters and all ground glass from the rotor the buckets the adapters and the rotor chamber You may need to replace the rubber mats and adapters to prevent further damage Fine splinters of glass may otherwise scratch the surface of the rotors and buckets reducing their resistance to c
25. d 1 3 Installing the device For 5804 R and 5810 R only To avoid damage to the compressor caused by incorrect transportation wait four hours after installation before switching on the device To disconnect the centrifuge from the power supply in the event of an error an emergency switch must be installed away from the centrifuge preferably outside the centrifugation room or next to the exit of this room Place the centrifuge onto a horizontal stable and resonance free work surface Ensure that the working environment is well ventilated and not exposed to direct sunlight There should be 15 cm clearance at the sides of the centrifuge and 10 cm to the rear According to the regulations of the EN 61010 2 020 standard a safety distance of 30 cm must be observed around the centrifuge during operation No objects which cause damage when destroyed must be positioned in this space Before plugging in the centrifuge compare your power supply with the electrical requirements listed on the identifica tion plate The mains cable of the centrifuge may be connected only to a socket with a protective conductor On refrigerated centrifuges 5804 R and 5810 R with a mains voltage of 120 V a distinction is made between two versions depending on requirements the 15 A variant is equipped with a conventional IEC power cable see Fig 3 so that these devices can be operated directly at the lab work station using a conventional sock
26. d The rotor must be loaded symmetrically Opposing tubes should be of the same type and should be filled equally For reasons of mechanical stabilization all positions of rotor A 4 44 and A 4 62 A 4 62 MTP must be loaded with buckets of the same type Prior to centrifugation the tubes should in any case be visually inspected for material damage Damaged tubes may not be centrifuged This is because broken tubes can in addition to sample loss result in further damage to the centrifuge and accessories Do not use buckets and rotors which show clear signs of corrosion or mechanical defects Please check accessories at regular intervals Do not operate centrifuges which have not been correctly installed or repaired Do not move or knock against the centrifuge during operation Repairs must only be performed by an Eppendorf authorized service technician Only use original rotors and spare parts recommended by Eppendorf The Centrifuges 5804 5810 and 5804 R 5810 R may only be used for special applications They must not be operated in a hazardous or flammable environment and must not be used to centrifuge explosive or highly reactive substances If such liquids are spilled in the rotor or rotor chamber the centrifuge must be cleaned with a moist cloth and a mild soap solution A liquid density of 1 2 g ccm must not be exceeded at the maximum rotational speed During longer spin times in the 5804 5810 models the sample tubes may heat
27. da la c mara del rotor se refrigera antes o despu s de una centrifugaci n a la temperatura nominal preseleccionada en tanto en cuanto esta temperatura est por debajo de la temperatura ambiente Selecci n de un programa s lo es posible cuando la centr fuga est parada AU confirmar una vez parpadea el numero de programa ajustado 0 datos del ltimo funcionamiento 1 9 A Z programas fijos Seleccionar el n mero de programa deseado con la centr fuga abierta regresa al programa 0 o sale del modo de programaci n Para iniciar el programa seleccionado debe cerrarse la tapa de la centr fuga antes de pulsar la tecla START STOP Instrucciones breves Programacion s lo es posible cuando la centr fuga est parada En primer lugar introducir los datos de programa deseados utilizando las teclas de par metros y las teclas cursoras Pulsar dos veces gt en la pantalla aparece con P el primer numero de programa libre y parpadea a y se puede seleccionar el n mero de programa libre deseado 1 9 A Z Mantener pulsada esta tecla durante 2 segundos hasta que aparezca ok en la pantalla Los par metros ajustados previamente Temp Speed Time etc est n ahora guardados como grupo de datos Interrumpe la programaci n en curso escape Si durante la centrifugaci n con programas fijos se modifican los par metros en el campo PROG aparece la indicaci n 0 y el usuario sale del programa
28. dad de 1 2 g ml en el material a centrifugar al n mero de revoluciones m ximo En caso de tiempos de marcha prolongados en las centr fugas 5804 5810 no refrigeradas se calentar n los recipientes de muestras Deben respetarse los datos limite especificados por el fabricante del recipiente en lo que se refiere a la capacidad de carga Si se emplean disolventes org nicos p ej Cloroformo la resistencia de los recipientes de pl stico puede disminuir Cuando se cambie de una sala refrigerada a una sala normal de laboratorio la centr fuga debe o bien funcionar para calentarse durante 1 2 h en la sala refrigerada o bien calentarse como m nimo durante 3 horas en la sala de laboratorio sin conectarse a la red con el fin de evitar da os debidos a la condensaci n Los rotores son componentes de alta calidad que deben resistir cargas extremas Los rotores de aluminio est n protegidos ampliamente contra la corrosi n producida por las sustancias qu micas habituales en los laboratorios mediante una capa de anodizaci n sin embargo esta protecci n no es ilimitada Por favor proteja los rotores frente al deterioro mec nico Incluso los peque os ara azos y grietas pueden producir graves da os internos al material que a simple vista son muy dif ciles o incluso imposibles de detectar Por favor evite la debilitaci n debida a sustancias qu micas agresivas entre las que se encuentran lcalis fuertes y d biles cidos fuertes solucione
29. de 5804 R 5810 R r frig r e Pictogramme des touches utilis es pour activer une fonction DD at set rpm R frig ration d attente Active la consigne vitesse le message SPEED clignote Active la commutation vers le message RZB de l acc l ration relative g ou du correcteur du rayon Q Modifie les valeurs num riques le cadran indique la nouvelle valeur de consigne Active la pr s lection du temps 1 99 min ou marche continue au cadran 00 Active la programmation des phases d acc l ration et de freinage voir tableau ZN Active la pr s lection de la temp rature pour la centrifugation et pour la r frig ration en attente Gamme de temp ratures 9 C 40 C Permet le d marrage de la centrifugation PY Clignote et indique que le rotor tourne D marre la reconnaissance manuelle du rotor Arr te la marche Interrompt la programmation en cours escape Efface le programme s lectionn D marre une r frig ration initiale rapide avec une vitesse de rotation sp cifique S arr te d s l obtention de la temp rature de consigne gt mission d un signal sonore p riodique Active le programme en m moire D marre une nouvelle programmation P1 P9 PA PZ D marre des centrifugations courtes Maintenir la touche short appuy e pour maintenir la centrifugation en marche L inscription SH s affiche l cran At set rpm entra ne un d comptage
30. de la dur e de la centrifugation lorsque le r gime pr s lectionn est atteint at set rpm gt En appuyant sur la touche START STOP pendant plus de 4 s lorsque le couvercle de la centrifugeuse est ouvert cela entra ne une commutation sur la fonction at set rpm Le couvercle du capot tant ferm l int rieur du bol du rotor sera r frig r soit avant soit apr s une centrifugation jusqu la temp rature de consigne si celle ci se situe en dessous de la temp rature ambiante S lection du programme possible uniquement l arr t de la machine a Actionner une fois gt le num ro du programme clignote 0 Donn es de la derni re marche 1 9 A Z Programme fixe S lectionner le num ro du programme souhait Avec le couvercle de la centrifugeuse ouvert l appareil revient au programme 0 et quitte le mode de programmation Pour commencer le programme s lectionn le couvercle de la centrifugeuse doit tre ferm avant d appuyer sur la touche START STOP Mode d emploi succint Programmation possible uniquement l arr t de la machine Permet d abord de composer le programme en utilisant les touches param tre et les touches fl ch es Actionner deux fois gt le premier n de programme libre et disponible se manifeste par le message P qui clignote a v Le num ro du programme libre souhait 1 9 A Z peut tre s lectionn Maintenir la touch
31. dividual deliveries please state the existing weight category 2 aerosol tight caps for 250 mL rectangular bucket 4 replacement seals for aerosol tight caps 22638602 22638637 22638661 22638670 22638700 22638718 22638726 22638742 22638769 22638921 22638785 22638904 22665201 22638696 22638653 22638807 22638840 22638866 22638882 22639005 22638980 22638998 22510070 22638009 22638025 22638033 22638017 7 Ordering information Adapters for standard test tubes blood vvithdravval symptoms and micro test tubes for 250 mL rectangular bucket Number and diameter of bores 2 adapters for 1 5 5 ml test tubes 25 x 11 22638203 2 adapters for 1 5 2 mL micro test tubes 16 x 11 22638220 2 adapters for 4 7 ml test tubes 15 x 13 22638246 2 adapters for 3 15ml test tubes 12 x 16 22638262 2 adapters for 7 17ml test tubes 12 x 17 5 22638301 2 adapters for 7 18mLtest tubes 8 x 20 22638327 2 adapters for 18 30 mL test tubes 4 x 26 22638360 2 adapters for 30 50 mL test tubes 4 x 31 22638386 2 adapters for 50 75 mL test tubes 2 x 35 22638408 2 adapters for 80 120 ml test tubes 1 x 46 22638424 2 adapters for 160 250 ml test tubes 1 x 62 22638441 4 spare rubber mats 22638483 4 spare rubber mats for adapter 22638441 22638459 2 spare clamps 22628467 Adapters for Falcon test tubes for 250 mL rectangular bucket Number and diameter of bores 2 adapters for 15 mL Falcon tes
32. dorf com Pour obtenir des renseignements propos de la m thode de d contamination appropri e veuillez vous adresser galement au responsable de la s curit de votre laboratoire Veuillez remplir Pattestation de d contamination et joignez la compl t e a l appareil lorsque celui ci doit tre renvoy la soci t Eppendorf Mode d emploi succint Tableau d identification des anomalies Centrifugeuses 5804 5804 R 5810 5810 R Anomalie Message Cause Rem de Absence message absent Probl me de raccordement V rifier raccordement secteur Alimentation d fectueuse V rifier fusibles sur appareil et sur tableau d alimentation du laboratoire Centrifugeuse ne Error 3 Absence rotor Installer un rotor d marre pas Error 23 Probl me sur entra nement ou sur le Faire une manoeuvre arr t marche dispositif de reconnaissance du rotor Capot impossible a absent Coupure secteur Attendre l arr t du rotor et ouvrir ouvrir manuellement le capot Fermeture incom Press Open Capot non verrouill Actionner touche Open puis refermer le pl te du capot capot Capot bloqu Lift Lid Le capot ne s ouvre pas de lui m me Soulever le capot Forte vibration de la IMBAL Chargement dissym trique du rotor V rifier les dispositions des tubes et leur centrifugeuse au chargement d marrage puis ROTOR Rotor desserr Serrer fermement le rotor conform ment arr t aux instruction
33. e F 45 48 PCR 6 x 8er PCR Streifen 12 000 15 350 9 5 e e Drum rotor T 60 11 60 x 2 ml 14 000 16 400 7 5 e e 3 Operating A 1 The swing bucket rotor can be delivered without buckets equipped with rectangular buckets as an A 4 81 or with MTP buckets as an A 4 81 MTP whereby the flex buckets especially MTP buckets can be purchased extra in sets of 2 or 4 user friendly change of rectangular buckets or MTP and flex buckets is guaranteed by the use of a common rotor cross 2 The swing bucket rotor can be delivered as A 4 62 loaded with buckets or as A 4 62 MTP loaded with microtest plates User friendly exchange of rectangular buckets or microtest plate buckets is guaranteed by the use of all in one rotor cross 9 A 4 81 is equipped with flex buckets and IsoRack adapters Swing bucket rotor AN Only the combinations of rotor buckets adapter which are recommended by the manufacturer may be used Please check whether all buckets have been inserted correctly and can swing freely For purposes of mechanical stabilization all positions of the rotors A 4 44 and A4 62 A 4 62 MTP must be loaded with the same buckets In contrast the rotor A 4 81 A 4 81 MTP can be equipped with a mix of rectangular buckets and titer plate buckets It is in principle not permitted to use swing bucket rotors with only two rectangular buckets or titer plate buckets inserted Fig 5 Rotor A 4 81 MTP
34. e activ e pendant 2 s jusqu l apparition de OK Les param tres r gl s pr c demment Temp temp Speed vitesse Time dur e etc sont pr sent m moris s Interrompt la programmation en cours escape Si certains param tres sont modifi s en cours de marche O appara t dans la zone programme et Putilisateur quitte le programme sans que celui ci soit modifi protection de l criture du programme Pour viter d effacer un programme par inadvertance les donn es pr c dentes devront tre annul es avant d assigner de nouvelles donn es une m moire programme avec le couvercle de la centrifugeuse ouvert Actionner une fois 3 la zone N de programme clignote a y Appeler le N de programme a effacer Maintenir en action dans les prochaines dix secondes jusqu a apparition du message cleared effac Ouverture de la centrifugeuse en cas de coupure de courant AN Mettre la touche secteur en position arr t Attendre ventuellement l arr t complet du rotor Introduire la cl du rotor dans l orifice situ au milieu de la fa ade La positionner dans la vis qui s y trouve tourner en sens anti horaire gauche Le capot sera ainsi d verrouill et pourra tre ouvert Retirer imp rativement la cl du rotor Nettoyage L appareil L ext rieur de la centrifugeuse ainsi que l int rieur du bol du rotor peuvent tre nettoy s a l aide d une solution d tergente neutre Nettoyer de tem
35. e medici n de Dejar el aparato conectado durante 8 min la velocidad de rotaci n hasta que se encienda la tecla Open y abrir la centr fuga a continuaci n Repetir la centrifugaci n Error 4 Sensor de bloqueo de la tapa Desconectar la m quina y volverla a averiado conectar Repetir la centrifugaci n Error 5 Apertura improcedente de la tapao Repetir la centrifugaci n interruptor de la tapa averiado overload Convertidor sobrecargado Freno Dejar enfriar y volver a iniciar al cabo o defectuoso de 5 min Error 6 sz f F a Tension de red demasiado baja Comprobar la tension de red Se ha aflojado el rotor Apretar el rotor Error 7 Exceso de revoluciones Repetir la centrifugacion Error 8 Rotor suelto Apretar el rotor Fallo en el accionamiento Motor defectuoso La centrifuga se overtemp Diferencia de temperatura gt 5 C Repetir la centrifugacion desconecta y se respecto al valor nominal escucha una sehal de alarma Error 9 25 Fallo del sistema electronico Clear Memory Memoria para programas llena Borrar algunos programas o iniciar una nueva centrifugaci n mediante Start o Prog Interrupt Fallo del suministro el ctrico durante la centrifugaci n Reiniciar El indicador de temperatura parpadea Diferencia de temperatura gt 3 C respecto al valor nominal Si las soluciones indicadas para la eliminaci n de la aver a no diesen resultado despu s de aplicarlas repetidamente por favo
36. e of the following two status signs will appear depending upon the rotor recognized see Chapter 3 16 Automatic rotor recognition rem max or 200 rpm The status displays mean rpm max The inserted rotor accelerates to its nominal rotational speed 200 rpm The maximum rotational speed for the short spin run can be set over the arrow keys If the Short key is pressed for longer than three seconds while the centrifuge lid is open the device will switch over to the other status and display it for two seconds The status set last will be maintained 13 bunje19dg Operating 00 Q 14 3 Operating 3 8 Time change during the run Press this key during the run not during the braking phase change the duration of the run The time elapsed is taken into account in the new actual value 3 9 Refrigeration for 5804 R 5810 R only 9 1060 Standby refrigeration The nominal temperature value can be set from 9 C to 40 C It can be modified during centrifugation Once the nominal temperature value has been reached a deviation greater than 3 C is indicated by a flashing temperature display If the temperature deviates by more than 5 C a periodic warning signal can be heard and the centrifuge switches itself off starts a temperature run at a rotor specific speed for the best possible refrigeration The new preselected temperature is quickly attained in the rotor by cooling FT appears in the PRO
37. ed SPEED appears in the display and the run must be restarted The rotational speed and the radius for the rcf value will be reset to the maximum permitted value If a run with a program has been started the program number is set to 0 3 18 Display of elapsed spin time If the Time key and the Prog key are pressed simultaneously the total running time in hours of the centrifuge appears in the display This function can only be selected when the rotor is at a standstill 3 19 Switching on off the warning signal Press the Speed and Time keys simultaneously Alarm on and Alarm off appear after 2 seconds speed alternately in the display 3 20 Exiting the Service program a y If the Service program has been selected accidentally press both arrow keys simultaneously 3 21 Control via serial interface optional All centrifuge functions can be operated via the serial interface RS 232 The appropriate conversion must be carried out by the service department Only devices which have been tested in accordance with IEC 950 may be connected via the serial interface 3 22 Opening the centrifuge in the case of a power failure If the magnetic lid latch cannot be activated because of a power failure the emergency lid release can be activated manually Turn off the main power switch Wait until the rotor has come to a standstill The rotor may continue spinning for up to eight minutes Insert the standard rotor key into the openi
38. epos avec son couvercle serr a fond Bris de verre Lors de la centrifugation de tubes en verre il faut savoir que le risque de bris de verre s accroit au fur et mesure que la vitesse de rotation ou de centrifugation augmente Veuillez respecter les indications du fabricant relatives a la charge maximale des tubes de centrifugation En cas de bris de verre veuillez retirer imm diatement tous les clats et le verre pulv ris du rotor des tubes des adaptateurs et de l espace de la centrifugeuse Eventuellement les plaques en caoutchouc et les adaptateurs doivent tre chang s pour viter davantage de d g ts Sinon les petits bris de verre rayent la surface des rotors et des b chers et leur compatibilit chimique s en trouve ainsi r duite Dans l espace de centrifugation des d chets d abrasion de m tal tr s fins noirs apparaissent qui provoquent outre des dommages sur la centrifugeuse les rotors les b chers et les adaptateurs galement une contamination des chantillons Veuillez contr ler r guli rement qu il ne se trouve pas de r sidus ou de dommages sur les alv oles des rotors Renvoi d appareils Lors du renvoi de centrifugeuses veuillez prendre garde a ce que les appareils soient enti rement d contamin s et qu ainsi ils ne pr sentent aucun risque pour la sant de notre personnel d entretien Vous trouverez d autres informations et un mod le de l attestation de d contamination sous www eppen
39. et 120 V 15 A However this causes a drop in the cooling performance of this kind of device This technical specification leads to a rise in the lowest temperature which can be reached at maximum speed and to slower cooling down to the specified value 1 Introduction Fig 3 15 A IEC power cable Fig 4 20 A standard variant On the 20 A standard variant by contrast the power cable is permanently attached to the device see Fig 4 These devices have a special 20 A plug and require the appropriate socket to guarantee the supply of current and voltage required for the centrifuges 120 V 20 A The advantage is greater more rapid cooling To switch on the centrifuge press the mains switch on the right hand side of the device The nominal values of the test run appear in the display and the control lamp in the Open key lights up The centrifuge lid can be opened by pressing the Open key Please insert the rotor before starting and tighten it with the supplied rotor key The rotors A 4 81 and A 4 81 MTP are tightened with the special rotor key included in the rotor delivery package uog npou Safety regulations and applicational limitations 2 Safety regulations and applicational limitations In the interests of your own personal safety always observe the following regulations AN The rotor and the rotor cover must alvvays be securely fastened Do not begin centrifugation before the rotor has been securely fastene
40. for different rotors for the levels 0 to 9 in seconds for 230 V centrifuges 5804 5804 R 5810 5810 R Rotor 0 1 2 3 4 5 6 7 8 9 O A 4 81 532 189 174 143 131 109 95 85 59 31 e A 4 81 MTP Flex 643 191 174 142 131 110 94 83 58 30 e A 4 62 740 190 170 140 130 110 95 85 55 26 e A 4 62 MTP 620 190 170 140 130 110 95 85 55 26 O Oo A 4 44 470 300 270 220 200 140 100 75 45 23 e e A 2 DWP 304 174 130 118 100 wa 51 44 32 14 e e T 60 11 800 280 140 95 70 55 45 40 36 36 e e F 34 6 38 880 370 280 190 170 150 125 95 75 54 e e F 45 30 11 240 140 70 45 35 30 25 22 19 18 e e FA 45 30 11 240 140 70 45 35 30 29 22 19 18 e o F 45 48 PCR 169 119 60 41 31 26 22 19 17 16 The given times are to be considered guidelines While level 9 means strongest braking level O means free deceleration whereby considerable fluctuations can occur with this uncontrolled deceleration depending upon the condition of the device and the load The deceleration times for the 230 V and 120 V devices are almost identical 3 12 At set rpm The at set rpm function triggers a counting down of the centrifugation time upon achieving the preselected rotational speed at set rpm Pressing the START STOP key for longer than 4 seconds while the centrifuge lid is open triggers a switching over to the at set rom function While the key is pressed both triangles of the pictogram are illuminated
41. hemicals The air turbulences within the rotor chamber produce a very fine black powder of abraded metal In addition to causing damage to the rotor chamber rotor buckets and adapters the powder also contaminates the samples Check the rotor bores regularly for residues and damage 4 6 Refrigerated centrifuges Clean the refrigeration mesh of the heat exchanger on the rear side of the device with a brush at least twice a year Be sure to first switch off the centrifuge and pull the plug Switch off the centrifuge after use leave the lid open and empty the tray for condensation water situated below the device on the front right hand side 4 7 Return of devices When returning centrifuges please ensure that the devices have been decontaminated and thereby do not present a health risk to our Service staff You will find additional information and a blank of the decontamination confirmation at www eppendorf com Do also consult your laboratory safety officer about a suitable decontamination method Please fill out the decontamination confirmation and place it together with the device when it is to be sent back to Eppendorf 18 Buiuesp pue 3DUBUIJUILLIA Troubleshooting 20 5 Troubleshooting Centrifuges 5804 5810 and 5804 R 5810 R Error Display Cause Solution No display None No main power connection Check power supply cable Power failure Check main power fuse on the device
42. ith rotational speed regulation up to 14 000 rpm 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Centrifuge 5804 R Refrigerated bench top centrifuge max capacity 4 x 100 mL with rotational speed regulation up to 14 000 rpm Temperature range 9 to 40 C 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Other voltages or 50 Hz frequency upon request Captain Eppi Rotor key holder 1 piece Captain Eppi Rotor key holder 10 pieces Rotor stand for all rotors for Centrifuges 5804 5804 R and 5810 5810 R 22622501 22622552 22623508 22623559 22639609 22639625 22639021 Swing bucket rotor and accessories see brochure for further details for Centrifuge 5804 5804 R Swing bucket rotor A 4 44 4 x 100 mL with 4 rectangular buckets of 100 mL 1 rectangular bucket of 100 mL with weight category for individual deliveries please state the existing weight category 2 aerosol tight caps for 100 mL rectangular bucket 4 replacement seals for aerosol tight caps of the rectangular bucket 100 mL Adapters for standard test tubes blood withdrawal systems and micro test tubes for 100 mL rectangular bucket Number and diameter of bores 2 adapters for 3 5mLtest tubes 14 x 11 2 adapters for 1 5 2 mL micro test tubes 12 x 11 2 adapters for 4 7 ml test tubes 12 x 13 3 7 2 adapters for 15 mL test tubes 7 x 16 2 adapters for 17 mL test tubes 6
43. l rotor s lo debe equiparse sim tricamente los recipientes que se encuentren unos frente a otros deben ser del mismo tipo y estar igual de llenos Por razones de estabilizaci n mec nica todas las posiciones de los rotores A 4 44 y A 4 62 A 4 62 MTP tienen que estar equipadas con dispositivos suspensores del mismo tipo Antes de proceder a la centrifugaci n deben inspeccionarse siempre visualmente los recipientes para identificar posibles da os materiales Los recipientes deteriorados no deben centrifugarse ya que su rotura adem s de la p rdida de la muestra podr a tener como consecuencia mayores da os en la centr fuga y los accesorios No deben emplearse vasos ni rotores que presenten claros signos de corrosi n o deterioros mec nicos Por favor controle los accesorios con regularidad No se deben poner en funcionamiento centr fugas no instaladas o no reparadas adecuadamente Durante el funcionamiento no debe moverse ni golpearse la centr fuga Los trabajos de reparaci n s lo debe realizarlos el personal de servicio autorizado por la empresa Eppendorf S lo deben emplearse piezas de recambio y rotores originales de la empresa Eppendorf Las centr fugas 5804 5804 R y 5810 5810 R deben emplearse nicamente para los casos de aplicaci n indicados No deben ponerse en funcionamiento en locales con riesgo de explosi n y no deben centrifugarse sustancias explosivas ni de reacci n fuerte No debe sobrepasarse la densi
44. limpieza con detergente las juntas de goma de la c mara del rotor deben enjuagarse bien con agua y frotarse con glicerina En caso de que se produzca condensaci n de agua en la c mara del rotor s quela con materiales absorbentes suaves 35 SIAIIG SIUOLDINASUL Instrucciones breves 36 Instrucciones breves Rotor AN No deben emplearse rotores deteriorados ni corroidos El anillo magn tico fijado en la parte inferior de cada rotor para la identificacion del rotor y el control del numero de revoluciones debe sustituirlo el servicio t cnico en caso de que se produzca alg n deterioro Cuando se utilicen rotores basculantes asegurarse de que las ranuras y los pivotes de los contenedores est n limpios y engrasados con la grasa para pivotes suministrada de tal forma que los contenedores puedan bascular con total libertad El rotor antiaerosol no debe guardarse con la tapa apretada Rotura de vidrios Para el centrifugado de tubos de vidrio se debe tener en cuenta que si aumenta el n mero de revoluciones o la aceleraci n centr fuga relativa crece el peligro de rotura del vidrio Por favor tenga en cuenta las instrucciones del fabricante sobre la carga m xima de tubos de centr fuga Si se produce una rotura de vidrio por favor extraiga todos los fragmentos y polvo de vidrio del rotor los contenedores los adaptadores y la c mara de centrifugaci n En caso necesario deben cambiarse por otros nuevos las placas de gom
45. load Converter overloaded Allow to cool Restart device after 5 min or Brake defective Error 6 Voltage supply too low Check voltage supply Rotor becomes loose Tighten rotor Error 7 Overspeed Repeat run Error 8 Rotor loose Tighten rotor Drive error Motor defective Centrifuge switches overtemp Temperature deviation from Repeat run off Warning signal nominal value gt 5 C Error 9 25 Electronics error Clear Memory Program memory full Delete several programs or press Start or Prog to begin new run Interrupt Power failure during the run Restart Flashing temperature display Temperature deviation from nominal value gt 3 C If the above solutions are unsuccessful please contact SERVICE 6 Technical data Centrifuges Power supply Maximum power requirement 5804 5810 5804 R 5810 R Fuse protection 5804 5810 5804 R 5810 R Max rotational speed Max centrifugal force Max kinetic energy 5804 5810 5804 R 5810 R Max load Max density of material to be centrifuged Permitted ambient temperature during operation 5804 5810 5804 R 5810 R Permitted maximum relative air humidity Degree of contamination Overvoltage category Standardized interface optional Noise level Dimensions W x D x H 5804 5810 5804 R 5810 R Weight 5804 5810 5804 R 5810 R Electrical data for 120 V model Power supply Maximum power
46. mL test tubes 1 x 29 2 adapters for 50 mL Falcon test tubes 1 x 29 5 2 adapters for 15 mL Falcon test tubes 1 x 17 Fixed angle rotor 30 x 1 5 mL type F 45 30 11 with rotor lid Spare lid for rotor F 45 30 11 Fixed angle rotor 30 x 1 5 mL type FA 45 30 11 aerosol tight with rotor lid of aluminium Spare lid for rotor FA 45 30 11 6 adapters for 0 4 mL centrifuge tubes 6 adapters for 0 5 mL micro test tubes and Microtainers 6 adapters for 0 2 mL PCR tubes PCR strip rotor F 45 48 PCR for 6 x 8 strips 6 x 5 strips 48 x 0 2 mL PCR tubes Drum rotor and accessories see brochure for the centrifuges 5804 5804 R 5810 5810 R Drum rotor T 60 11 with rotor lid for 60 x 1 5 mL 2 mL tubes 7 120 x 0 4 mL tubes with 6 adapters for 1 5 mL 2 mL tubes Adapter for 1 5 mL 2 mL tubes set of 6 Adapter for 0 4 mL tubes set of 6 Accessories for the Centrifuges 5804 5804 R Grease for pivots Standard rotor key Conversion kit for interface for 5804 Conversion kit for interface for 5804 R Tray for condensation water Important note Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such actio Rotor code Fixed angle Angle of Tube rotor adapter bore Adapter Swing bucket rotor All Ep
47. mediately using a neutral cleaning liquid This is particularly important for the bores of the fixed angle rotor and for the buckets Please clean your rotor regularly using a neutral cleaning liquid e g Extran neutral or RBS neutral This will protect the rotor and prolong its service life AN According to EN 55011 the centrifuges 5804 5804 R and 5810 5810 R are Class A products Interference with signal reception can occur in residential areas The operator should take appropriate protective measures 2 Safety regulations and applicational limitations The follovving rotors and accompanying buckets have a maximum operating life of seven years The date of production is engraved on the rotor in four digit form e g 10 98 October 1998 A 4 44 5804 730 003 A 4 62 MTP 5810 711 002 A 2 DWP 5804 740 009 T 60 11 5804 730 003 F 34 6 38 5804 727 002 A 4 81 5810 718 007 A 4 62 5810 709 008 A 4 81 MTP 5810 725 003 Transparent rotor lids made of PC and PP as well as the PC caps of the rectangular buckets have an operating life of three years The date of production is engraved in the form of a clock AKS e LF Contact with organic solvents e g phenol chloroform may have an adverse effect on the transparent polycarbonate caps of the aerosol tight rotors Please check lids of this type regularly for chemical damage or for cracks Cracked caps or caps with a milky discoloration must be replaced immediately A Do not use rotors ca
48. ng in the middle of the front part in the nut underneath and turn counter clockwise This disengages the lid allowing it to be opened Be absolutely sure to remove the rotor key aftervvards 3 23 Overload cutout switch 7 Fuses The 230 V and 120 V devices have built in thermal overload switches which function as all pole fuses When the overload protection is actuated these switches over the power switch to OFF but do not switch it on again automatically To turn on the overcurrent protector switch again please turn off the centrifuge for 10 seconds with the mains power switch When the centrifuge is subsequently turned on again the overcurrent protector switch will be automatically reactivated 17 bunje19dg Maintenance and cleaning 18 4 Maintenance and cleaning 4 1 Device AN The outside of the centrifuge and the rotor chamber should be cleaned regularly vvith neutral detergent This is for hygiene purposes as vvell as to prevent contamination caused by residual contamination The user is responsible for cleaning or decontaminating the centrifuge in the event of contamination caused by high risk substances Open the lid of the centrifuge and disconnect the main power plug Unscrew the rotor with the rotor key provided and clean separately Only neutral agents may be used for cleaning and disinfection e g diluted Extran neutral RBS neutral or 70 isopropanol water mixture or an alcohol based disinfectant The rotor ch
49. or recognition see also Chapter 3 16 occurs at 200 to 700 1 min and the device then accelerates to the nominal speed During the run the rotational speed of the rotor or the appropriate rcf value the sample temperature and the remaining spin time in minutes are displayed The last minute is counted down in seconds During the run all parameters can be modified After the end of a run or after a run has been interrupted by pressing the Stop key the rotor is braked and brought to a standstill During the braking process the time display flashes and the elapsed spin time is displayed When the lamp in the Open key lights up the lid can be unlocked by pressing the key 3 6 Continuous operation The continuous operation function is set above 99 min or below 1 min using the arrow keys In the time display oo indicates continuous operation During the run the actual rotational speed RCF and temperature values and the elapsed time in minutes are displayed If the centrifuge runs for more than 99 min 99 appears in the display The run is ended with the STOP key 3 7 Short spin centrifugation Short spin Press for as long as the run should last The text SH will appear in the display The elapsed time is displayed in seconds An already interrupted run can be continued up to two times by repeatedly pressing the key as long as the centrifuge has not yet come to a stillstand If the Short key is pressed while the lid is open on
50. pendorf rotors are designated l l l l according to a simple logical system which describes the technical specifi F A 45 e 30 1 1 A cations as a uniform series of numbers I I and letters e g 22637207 22662961 22637215 22637223 22637231 22637240 22637258 22637266 22637274 22637002 22662970 22637100 22637126 22636243 22636227 22636260 22638581 22638505 22638521 22638548 22634330 22664166 Upon request Upon request 22662678 n tube adapter bore 4 81 Aerosol tight Max no tubes Max no tubes version adapters adapters 23 uoyeu1ojulr BUL3pP10 Ordering information 24 7 Ordering information Centrifuge 5810 Bench top centrifuge max capacity 4 x 400 mL vvith rotational speed regulation up to 14 000 rpm 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Centrifuge 5810 R Refrigerated bench top centrifuge max capacity 4 x 400 mL with rotational speed regulation up to 14 000 rpm Temperature range 9 to 40 C 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Other voltages or 60 Hz frequency upon request Rotor stand for all rotors for Centrifuges 5804 5804 R and 5810 5810 R 22625004 22625055 22625501 22625551 22639021 Swing bucket rotor and accessories see brochure for further details for Centrifuges 5810 5810 R Swing bucket rotor A 4 81 4 x 400 mL with 4 rectangular bucke
51. ps a autre le rotor les nacelles et les b chers de mani re a liminer les souillures et d ventuels d p ts qui risqueraient d endommager les mat riaux Les seuls produits de nettoyage admis sont les produits nettoyants et d sinfectants neutres exemples Extran ou solution eau thanol a 70 L int rieur du bol du rotor ne devra tre nettoy qu a l aide d un tissu doux et l g rement humidifi Apr s chaque nettoyage au d tergent les joints caoutchouc de l int rieur du bol du rotor seront bien rinc s l eau ensuite trait la glyc rine En cas de d p t de condensats l int rieur du bol du rotor s cher avec un chiffon doux et absorbant 29 juLons 10 dW3 p 2poN Mode d emploi succint 30 Mode d emploi succint Rotor AN Il est interdit d utiliser des rotors ou des b chers endommag s ou ayant subi une corrosion Le rotor comporte un anneau magn tique permettant son identification et le contr le du r gime Il est fix a la base de ce rotor En cas d endommagement il devra tre remplac en atelier par un personnel qualifi Les rotors libres devront subir une attention particuli re en veillant la stricte propret des articulations recevant les nacelles et les b chers Elles devront tre nettoy es et lubrifi es l aide de la graisse pour articulation fournie avec l appareil Les nacelles doivent pouvoir osciller librement Le rotor anti a rosols ne devra jamais tre entr
52. ps or buckets which have been subjected to chemical or mechanical damage or which have exceeded their maximum operating life PC Polycarbonate PP Polypropylene suorjejtuulj jeuonjesrdde pue suoye n631 Aes Operating 10 3 Operating 3 1 Operating controls See the fold back cover at the front of this manual In the text etc signify keys Buckets include buckets and titer plate buckets 3 2 Mounting dismounting the rotors A summary of the various different rotors can be found in the brochure Clean the motor axle and the rotor bores with a cloth before attaching the rotor When fastening loosening the rotor onto from the motor axle ensure that the temperature of the rotor and the motor axle is between 10 C and 30 C Mount the rotor onto the motor axle and tighten the rotor nut by turning clockwise using the appropriate rotor key supplied To dismount the rotor turn the rotor nut counterclockwise using the rotor key When the rotors are not in the centrifuge please place them in the rotor stand Order no 5804 720 008 Do not centrifuge using rotors and buckets with visible corrosion or mechanical defects see Chapter 2 Safety regu lations and applicational limitations The Centrifuges 5804 5810 and 5804 R 5810 R have automatic rotational speed limitation 3 3 Rotor lid F 45 30 11 The non aerosol tight rotor lid of the rotor F 45 30 11 is attached by pressing
53. r p ngase en contacto con el SERVICIO T CNICO 37 SIAIIG SIUODINASUL Eppendorf Offices AUSTRALIA NEW ZEALAND Eppendorf South Pacific Pty Ltd Tel 61 2 98 89 50 00 Fax 61 2 98 89 51 11 E Mail Info eppendorf com au Internet www eppendorf com au AUSTRIA Eppendorf Austria Tel 43 1 2901756 0 Fax 43 1 2901756 20 E Mail office eppendorf at Internet www eppendorf at BRAZIL Eppendorf do Brasil Ltda Tel 55 11 3095 9344 Fax 55 11 3095 9340 E Mail eppendorf eppendorf com br Internet www eppendorf com br CANADA Eppendorf Canada Ltd Tel 1 905 826 5525 Fax 1 905 826 5424 E Mail canada eppendorf com Internet www eppendorfna com CHINA Eppendorf China Ltd Tel 86 21 68760880 Fax 86 21 50815371 E Mail market info eppendorf cn Internet www eppendorf cn CZECH REP 8 SLOVAKIA EPPENDORF Czech 8 Slovakia s r o Tel 420 323 605 454 Fax 420 323 605 454 E Mail eppendorf eppendorf cz Internet eppendorf cz Internet eppendorf sk FRANCE EPPENDORF FRANCE S A R L Tel 33 1 30 15 67 40 Fax 33 1 30 15 67 45 E Mail eppendorf eppendorf fr Internet www eppendorf fr GERMANY Eppendorf Vertrieb Deutschland GmbH Tel 49 2232 418 0 Fax 49 2232 418 155 E Mail vertrieb eppendorf de Internet www eppendorf de INDIA Eppendorf India Limited Tel 91 44 42 11 1314 Fax 91 44 42 18 74 05 E Mail info eppendorf co in Internet www eppendorf co in ITALY
54. re p t a ed da T t 5 Mode d emploi SUCCINCE 000 a a e r ear 26 32 Instrucciones DIEVES oia anne dua dues endow ei r No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2005 Eppendorf AG Hamburg Centrifuge 5804 5804 R 5810 5810 R Contents 1 Introduction s s a a A A A A A AA a r 6 1 1 Delivery package iii a IA A dls 6 1 2 Unpaid A A A et ade TA a A eee Be 6 1 3 Installingithe device ci aia a beh A dvd L 6 2 Safety regulations and applicational limitations 8 3 Gj rs a dace o ace 10 3 1 Operating CONTOS miii A A Pee wee Ree eee Hewes 10 3 2 Mounting dismounting the rotors aaa han 10 3 3 Rotor lid F 45 30 coi e E sr A Ad 10 3 4 Loading th FOLOTS an s h k d Vo o ed indie l 10 3 5 Routine centrifugation with preset time 7 rcf display 12 3 6 Continuous operation dc sis ve ts d md K e de due die ee Gi r a 13 3 7 Short spin c ntriftugation SHOK SIM sa cia nis rica a a ee ae 13 3 8 Tim sehang during th TUM c soc du j den a A ken eh oe gee 14 3 9 Refrigeration for 5804 R 5810 R only 14 3 10 Preset TACIUS ss juve nr ere nied seep Ahead akin ove hd ten ete Dna wade dde ae nan du db 15 3 11 Centrifugation with Soft start stop ee eee eee eee eee 15 3 12 ASETI JIM e e r
55. requirement 5804 5810 5804 R 5810 R 20 A version 5804 R 5810 R 15 A version Fuse projection in the device 5804 5810 5804 R 5810 R 20 A version 5804 R 5810 R 15 A version Technical specifications subject to change 5804 5804 R 230 V 50 or 60 Hz 900W 1650 W Excess current switch 12 A Excess current switch 12 A 14 000 rem 20 800 rcf 19 000 Nm 11 000 rpm 19 000 Nm 11 000 rpm 4 x 100 mi 1 2 g ml 2 C to 40 C 15 C to 35 C 75 2 Il RS 232 C lt 65 dB A 466 x 496 x 337 mm D 550 with operating section 634 x 496 x 342 mm D 550 with operating section 55 kg 80 kg 120 V 60 Hz 950 W 1650 W 1300 W Excess current switch 12 A Excess current switch 18 A Excess current switch 15 A 5810 5810 R 230 V 50 or 60 Hz 900 W 1650 W Excess current switch 12 A Excess current switch 12 A 14 000 rpm 20 800 rcf 19 000 Nm 11 000 rpm 23 000 Nm 12 000 rpm 4 x 400 ml 1 2 g ml 2 C to 40 C 15 C to 35 C 75 2 Il RS 232 C lt 65 dB A 535 x 536 x 345 mm D 608 with operating section 700 x 536 x 345 mm D 608 with operating section 68 kg 99 kg 120 V 60 Hz 950 W 1650 W 1300 W Excess current switch 12 A Excess current switch 18 A Excess current switch 15 A 21 ejep jeoiuyoal Ordering information 22 7 Ordering information Centrifuge 5804 Bench top centrifuge max capacity 4 x 100 mL w
56. s Machine bouscul e support de base Poser la centrifugeuse sur un support non stable stable Centrifugeuse SPEED R gime de consigne trop lev Programmer une vitesse compatible s arr te Error 1 D faut de reconnaissance du rotor Relancer la machine si m me message changer de rotor Error 2 D tecteur de balourd d fectueux Refaire essai Error 3 D faut dans le syst me Laisser reposer la machine sous tension compte tours pendant 8 min et attendre que le t moin Open s allume puis ouvrir Refaire essai Error 4 D tecteur de verrouillage du capot Faire une manoeuvre arr t marche d fectueux Refaire essai Error 5 Ouverture du capot non compatible Refaire essai ou commutateur de capot d fectueux overload Convertisseur en surcharge Refaire essai apr s 5 min ou frein d fectueux Error 6 Rotor d fait Resserrer rotor Error 7 Surr gime Refaire essai Error 8 Rotor d fait Resserrer rotor D faut dans entrainement Moteur d fectueux Error 9 25 Pannes d lectronique La centrifugeuse overtemp Ecart de temp rature sup rieur Refaire essai s arr te avec mission d alarme 5 C sonore Clear Memory M moire programme satur e Effacer certains programmes Temp rature cart de temp rature gt 3 C clignotante sur la consigne Si aucune des mesures pr conis es ci dessus ne devait porter de rem de aux anomalies il convient de faire appel au service apr s vente Eppendorf
57. s con iones de mercurio de cobre y de otros metales pesados hidrocarburos clorurados soluciones salinas concentradas Al manipular l quidos marcados como t xicos o radiactivos o g rmenes pat genos del grupo de riesgo Il ver Organizaci n Mundial de la Salud Laboratory Biosafety Manual Manual de bioseguridad en laboratorios deben observarse las respectivas disposiciones nacionales Instrucciones breves En caso de contaminaci n por medios agresivos por favor limpie el rotor inmediatamente con un detergente neutro Esto es especialmente v lido para los orificios de los rotores de ngulo fijo y los vasos Los siguientes rotores y los correspondientes vasos dispositivos suspensores tienen una vida til m xima de 7 a os la fecha de fabricaci n est estampada en forma 10 98 oct 1998 A 4 44 5804 730 003 A 4 62 MTP 5810 711 002 A 2 DWP 5804 740 009 T 60 11 5804 730 003 F 34 6 38 5804 727 002 A 4 81 5810 718 007 A 4 62 5810 709 008 A 4 81 MTP 5810 725 003 Las tapaderas de rotores as como las caperuzas de los vasos rectangulares de PC o PP tienen una vida til de 3 a os La fecha de fabricaci n est estampada en forma de reloj Las caperuzas transparentes pueden perder su resistencia debido al efecto de los disolventes organicos fenol cloroformo Por favor controle peri dicamente dichas caperuzas con respecto a ataques qu micos o peque as grietas Las caperuzas con grietas o con coloraciones lechosas deben s
58. sin que quede modificado protecci n contra sobreescritura Para evitar borrar por error un programa existente en caso de que la tapa de la centr fuga est abierta antes de asignar nuevos datos a un n mero de programa debe borrarse el grupo de datos antiguo Pulsar una vez gt La pantalla numero de programa parpadea a y Selecci n del n mero de programa a borrar Mantener pulsada esta tecla durante 2 segundos hasta que aparezca la palabra cleared borrado Apertura de la centrifuga en caso de fallo del suministro el ctrico apertura de emergencia AN Desconectar el interruptor de red Debido a que el rotor podria seguir funcionando esperar a que el rotor se pare Introducir la llave del rotor en la tuerca debajo del orificio situado en el centro de la parte frontal de la centrifuga y girar en el sentido contrario a las agujas del reloj La tapa se desbloquea y se puede abrir A continuacion sacar obligatoriamente la llave del rotor Limpieza Aparato La superficie exterior de la centr fuga y la c mara del rotor pueden limpiarse con un detergente neutro De vez en cuando deben limpiarse el rotor y los vasos con el fin de evitar que los restos del material centrifugado provoquen modificaciones en el material Para la limpieza y la desinfecci n deben emplearse nicamente agentes neutros por ejemplo Extran o etanol al 70 La c mara del rotor debe limpiarse nicamente con un pa o h medo Despu s de la
59. t tubes 9 x 17 5 22638289 2 adapters for 50 mL Falcon test tubes 3 x 31 22638343 2 adapters for 50 mL Falcon test tubes 4 x 31 22638351 Swing bucket rotor 4 x 100 mL type A 4 44 see 5804 5804 R and brochure Swing bucket rotor A 4 62 MTP with 4 plate carriers 22638041 4 plate carriers for swing bucket rotor A 4 62 MTP for 4 plates or 1 deepwell plate 22638068 1 plate carriers for swing bucket rotor A 4 62 MTP with weight category individual delivery only as a replacement delivery with prior agreement 22638076 Deepwell plate rotor A 2 DWP with 2 DWP buckets for deepwell plates 22638564 Deepwell plate bucket with weight category for swing bucket rotor A 2 DWP individual delivery only as a replacement delivery with prior agreement 22638572 Fixed angle rotors and accessories see 5804 5804 R and brochure Drum rotor and accessories see 5804 5804 R and brochure Accessories for the Centrifuges 5810 5810 R Grease for pivots 22634330 Standard rotor key 22664166 Conversion kit for interface for 5810 Upon request Conversion kit for interface for 5810 R Upon request Tray for condensation water 22662678 Important note Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such action N uoneuiojut BUL PIO
60. torio CLEAn ro 200 marchas Limpiar el rotor y el recipiente Standby off 8 h no utilizado Abrir la tapa y volverla a cerrar La centr fuga no no rotor No hay rotor Colocar un rotor centrifuga ee Fallo en el accionamiento o en la Desconectar la maquina y volver a identificacion del rotor iniciarla No se puede abrirla Nada Fallo en el suministro el ctrico Dejar que el rotor se detenga y activar la tapa apertura de emergencia de la tapa La tapa no se cierra Press Open La tapa no esta bloqueada Pulsar la tecla Open y a continuaci n totalmente cerrar de nuevo la tapa La tapa no se abre Lift Lid La tapa no se abre autom ticamente Abrir la tapa manualmente Instrucciones breves Error Pantalla Causa Eliminacion La centrifuga vibra IMBAL El rotor no se ha cargado Controlar el equipamiento y la carga del durante la puesta en sim tricamente rotor marcha y se ROTOR El rotor no se ha apretado bien Apretar bien el rotor desconecta La m quina ha recibido un golpe la Colocar la m quina sobre una mesa fija mesa es inestable La centr fuga se SPEED Velocidad nominal demasiado alta Controlar el equipamiento y la carga del desconecta para el rotor rotor Error 1 No se reconoce el rotor Repetir la centrifugacion y en caso de que se vuelva a producir el error comprobar con otro rotor Error 2 Sensor de desequilibrio averiado Repetir la centrifugaci n Error 3 Aver a en el sistema d
61. tre interdit tout objet susceptible de comporter un risque par sa destruction Raccorder la centrifugeuse au secteur et la mettre sous tension par le commutateur principal Actionner la touche open pour ouvrir le capot Avant de d marrer la machine mettre en place le rotor et le serrer fermement l aide de la cl du rotor Les rotors A 4 81 et A 4 81 MTP sont serr s fermement avec la cl sp ciale du rotor faisant partie des l ments livr s avec les rotors Chargement du rotor Il convient de respecter les indications fournies dans le guide des accessoires Concernant le rotor libre AN v rifier le bon positionnement des nacelles ainsi que la libert de leur mouvement d oscillation Pour garantir la stabilit de l ensemble pour les rotors A 4 44 et A 4 62 A 4 62 MTP toutes les places doivent tre occup es par des nacelles identiques Par contre pour le rotor A 4 81 A 4 81 MTP un quipement mixte de gobelets rectangulaires et de plaques en titre suspendues est possible Lorsque des tubes de hauteur sup rieure a 100 mm sont utilis s il est imp ratif de v rifier manuellement et a vide leur possibilit d oscillation 27 yuns 10 dua p apo Mode d emploi succint 28 Mode d emploi succint El ments de fonction et de commande d plier la premi re et derni re page de couverture Fig 1 Fig 2 Cadran et clavier de commande 5804 5810 non r frig r e Cadran et clavier de comman
62. trifuga se abre pulsando la tecla Open Antes del inicio por favor coloque el rotor y apri telo con la llave del rotor Los rotores A 4 81 y A 4 81 MTP se aprietan con la llave especial del rotor que se incluye en el suministro de los rotores Funcionamiento Tenha em atenc o os dados constantes do guia da centr fuga Carga de los rotores Para los rotores basculantes se aplica AN Compruebe si todos los dispositivos suspensores estan colgados completamente y pueden bascular con total libertad Por razones de estabilizaci n mec nica en el caso de los rotores A 4 44 y A 4 62 A 4 62 MTP todas las posiciones tienen que estar ocupadas por dispositivos suspensores del mismo tipo Por el contrario en el caso del rotor A 4 81 A 4 81 MTP es posible una ocupaci n mixta con vasos rectangulares y dispositivos suspensores de placas Titer Si se utilizan tubos muy largos mas de 100 mm de longitud es imprescindible realizar un test de basculaci n manual con contenedores vac os 33 SIAIIG SIUOLDINASUL Instrucciones breves 08 600 34 Instrucciones breves Elementos de funcionamiento y control Consultar la p gina desplegable de este manual Fig 1 Pantalla y panel de control del modelo 5804 5810 no refrigerada Fig 2 Pantalla y panel de control del modelo 5804 R 7 5810 R refrigerada i etc indican teclas que se utilizan para activar funciones U at set rpm Refrigeraci n en standby
63. ts of 400 mL 1 rectangular bucket of 400 mL with weight category for individual deliveries please state the existing weight category 2 aerosol tight caps for 400 mL rectangular bucket Replacement cap sealings Adapters for standard test tubes Vacutainers and Falcons for 400 rectangular bucket 2 adapters for 2 6 7 mL 25 x 13 number and diameter of bores 2 adapters for 5mL Monovette 18 x 13 2 adapters for 7 17 mL 16x 17 5 2 adapters for 5 mL Falcon 12 x 7 5 2 adapters for 50 mL Falcon 5 x 31 2 adapters for 180 250 mL tubes 1 x 62 2 adapters for 400 mL 1 x 81 2 adapters for Centriprep Centrifugal Filter Units 5 x 31 Replacement adapter rubber mats Replacement adapter clamps 2 bottles 400 mL Swing bucket rotor A 4 81 MTP with 4 MTP Flex buckets 4 MTP Flex buckets for swing bucket rotor type A 4 81 MTP or A 4 81 for use of IsoRack adapters cell culture flasks adapters and MTP DWP 2 MTP 7 Flex buckets 1 MTP Flex bucket 2 adapters for each cell culture flask for use in the MTP Flex buckets 2 adapters for each IsoRack for 0 5 ml micro test tubes 2 adapters for each IsoRack for 1 5 and 2 0 ml micro test tubes 1 IsoRack Starter Set 2 IsoRack adapters 2 IsoRacks with lid 2 x 0 C cool packs IsoRack for 0 5 ml and 1 5 ml 2 ml micro test tubes Swing bucket rotor A 4 62 4 x 250 mL with 4 rectangular buckets of 250 mL 1 rectangular bucket of 250 mL with weight category for in
64. ue et les tubes se trouvant les uns c t des autres doivent tre du m me type et identiquement remplis Pour assurer la stabilisation m canique tous les emplacements des rotors A 4 44 et A 4 62 A 62 MTP doivent tre dot s des m mes suspensions Avant d effectuer la centrifugation il convient de v rifier visuellement dans chaque cas un ventuel endommagement des r cipients Les r cipients endommag s ne doivent pas tre centrifug s puisque si ceux ci se cassent il peut y avoir outre la perte de l chantillon d autres dommages sur la centrifugeuse et les accessoires Les gobelets et les rotors qui pr sentent des traces nettes de corrosion ou des d t riorations m caniques ne doivent pas tre utilis s Veuillez contr ler r guli rement les accessoires Les centrifugeuses qui n ont pas t install es ou remises en tat par des sp cialistes ne peuvent pas tre utilis es La centrifugeuse ne peut pas tre d plac e ni secou e au cours du fonctionnement Les r parations ne peuvent tre effectu es que par le service apr s vente autoris par la soci t Eppendorf Seuls des pi ces de rechange et des rotors d origine de la soci t Eppendorf peuvent tre ins r s Les centrifugeuses 5804 5804 R ou 5810 5810 R ne peuvent tre utilis s que pour les cas indiqu s d utilisation Elles ne doivent pas tre utilis es dans des endroits soumis a un risque d explosion des mati res explosives ou hautement r
65. ustituirse inmediatamente No deben volver a utilizarse los rotores las tapas las caperuzas ni los dispositivos suspensores que est n deteriorados debido a efectos qu micos o mec nicos o que hayan superado su vida til PC Policarbonato PP Polipropileno Instalaci n del aparato S lo para el modelo 5804 R 5810 R Para evitar aver as en el compresor debido a un transporte inadecuado el aparato s lo debe conectarse cuando hayan transcurrido 4 horas desde la instalaci n La tension et la fr quence du r seau doivent correspondre aux donn es fournies sur la plaque du fabricant de l appareil La tension y frecuencia de red tienen que coincidir con los datos indicados en la placa de caracter sticas del aparato Coloque la centrifuga en una mesa de laboratorio horizontal estable y sin vibraciones las vibraciones fuertes de la mesa de laboratorio pueden dejar fuera de servicio el interruptor de desequilibrio El ambiente debe estar bien ventilado y protegido de los rayos solares Debe mantenerse una distancia de 15 cm a ambos lados y de 10 cm por detr s de la centr fuga Seg n recomienda la norma EN 61010 2 020 durante la centrifugaci n debe haber marcada una distancia de seguridad de 30 cm alrededor de la centr fuga en este espacio no deben encontrarse objetos cuya destrucci n pudiera causar da os Enchufe la centr fuga a la red y conecte el interruptor principal La tapa de la cen
66. x 17 5 2 adapters for 7 18 mL test tubes 4 x 20 2 adapters for 12 30 mL test tubes 2 x 26 2 adapters for 30 50 mL test tubes 1 x 31 2 adapters for 50 75 mL test tubes 1 x 35 2 adapters for 80 120 mL test tubes 1 x 46 4 spare rubber mats 2 spare clamps Adapters for Falcon test tubes for 100 mL rectangular bucket Number and diameter of bores 2 adapters for 15 mL Falcon test tubes 4 x 17 5 2 adapters for 50 mL Falcon test tubes 1 x 31 4 buckets for 50 mL Falcon test tubes 2 x 31 8 spare adapters for 50 ml Falcon tubes as re order Deepwell plate rotor A 2 DWP with 2 DWP buckets for deepwell plates Deepwell plate bucket with weight category for swing bucket rotor A 2 DWP individual delivery only as a replacement delivery with prior agreement 22637401 22637410 22637428 22637444 22637509 22637525 22637541 22637568 22637584 22637622 22637649 22637681 22637703 22637720 22662503 22662511 22637606 22637665 22637452 22637479 22638564 22638572 7 Ordering information Fixed angle rotors and accessories see brochure for the centrifuges 5804 5804 R 5810 5810 R Fixed angle rotor 6 x 85 mL type F 34 6 38 with rotor lid Spare lid for rotor F 34 6 38 2 adapters for micro test tubes 4 x 11 5 2 adapters for 7 15 mL test tubes 2 x 16 5 2 adapters for 15 18 mL test tubes 1 x 18 5 2 adapters for 20 30 mL test tubes 1 x 26 5 2 adapters for 50

Centrifuge 5804 / 5804 R / 5810 / 5810 R 1

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